Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(Dendrites ultrastructure 60). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters

1. Moore, Carlene Drucilla. The role of centaurin alpha-1 in the regulation of neuronal differentiation.

Degree: PhD, 2008, University of Alabama – Birmingham

In the nervous system, PI 3-kinase has been implicated in neuronal differentiation. My studies have focused on a candidate neuronal PI 3-kinase target centaurin alpha-1, which binds PtdIns(3,4,5)P3, and is an Arf6 GAP. Centaurin alpha-1 is localized in dendrites, dendritic spines and synapses, and is required for neuronal differentiation and spine morphogenesis. In dissociated neuronal cultures, expression of centaurin alpha-1 enhances dendritic branching, and increases dendritic filopodia, lamellipodia and spine-like protrusions. Expression of centaurin alpha-1 GAP inactive mutant or knocking down centaurin alpha-1 levels using siRNA leads to inhibition of dendritic outgrowth and branching. Manipulations of centaurin alpha-1 also disrupt spine morphogenesis in organotypic brain slice cultures. The effects of centaurin alpha-1 on dendritic development are dependent on it functioning through regulation of Arf6. The constitutively GTPbound mutant Arf6, which reduces dendritic branching on its own, is able to reverse the effects of centaurin alpha-1 overexpression. Conversely, expression of the GDP-bound mutant, Arf6, which enhances branching and outgrowth on its own, can prevent the loss of dendrites induced by centaurin alpha-1 GAP inactive mutant expression or siRNA knock down. Arf6 has been shown to regulate Rac1, and both Arf6 and centaurin alpha- 1 have been proposed to regulate ERK; both Rac1 and ERK have been implicated in neuronal differentiation. Thus, I examined whether centaurin alpha-1 modulates neuronal Rac1 and ERK, to identify candidate downstream effectors of centaurin alpha-1 and Arf6 in neuronal differentiation. ERK and Rac1 activation are enhanced in centaurin alpha-1 over-expressing neurons and this activation is dependent on centaurin alpha-1 GAP activity. As a regulator of Arf6, centaurin alpha-1 has emerged as a candidate to participate in PI 3-kinase regulated Arf6 pathways that control dendritic differentiation and spine morphogenesis.

xiv, 160 p. : ill., digital, PDF file

Neurobiology

Joint Health Sciences

Centaurin alpha-1 P13 kinase Arf6 Dendrites Rac 1 ERK1/2

UNRESTRICTED

Advisors/Committee Members: Theibert, Anne, Floyd, Candice <br>, Pozzo-Miller, Lucas <br>, Roth, Kevin <br>, Wilson, Scott.

Subjects/Keywords: 1-Phosphatidylinositol 3-Kinase <; br>; Adaptor Proteins, Signal Transducing  – metabolism <; br>; Dendrites  – metabolism <; br>; Dendrites  – ultrastructure <; br>; GTPase-Activating Proteins  – metabolism <; br>; Hippocampus  – cytology <; br>; Nerve Tissue Proteins  – metabolism <; br>; Neurons  – metabolism

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Moore, C. D. (2008). The role of centaurin alpha-1 in the regulation of neuronal differentiation. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,208

Chicago Manual of Style (16th Edition):

Moore, Carlene Drucilla. “The role of centaurin alpha-1 in the regulation of neuronal differentiation.” 2008. Doctoral Dissertation, University of Alabama – Birmingham. Accessed October 20, 2019. http://contentdm.mhsl.uab.edu/u?/etd,208.

MLA Handbook (7th Edition):

Moore, Carlene Drucilla. “The role of centaurin alpha-1 in the regulation of neuronal differentiation.” 2008. Web. 20 Oct 2019.

Vancouver:

Moore CD. The role of centaurin alpha-1 in the regulation of neuronal differentiation. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2008. [cited 2019 Oct 20]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,208.

Council of Science Editors:

Moore CD. The role of centaurin alpha-1 in the regulation of neuronal differentiation. [Doctoral Dissertation]. University of Alabama – Birmingham; 2008. Available from: http://contentdm.mhsl.uab.edu/u?/etd,208

2. Larimore, Jennifer Lynn. The role of centaurins in vesicular trafficking and neuronal differentiation.

Degree: PhD, 2008, University of Alabama – Birmingham

In order to better understand the role of the phosphoinositide (PI) 3-Kinase (PI3K) pathway in neuronal differentiation and plasticity, I am studying two centaurins that are Arf GAPs and candidate targets downstream of PI3K. Arfs are small GTPases that regulate vesicular membrane trafficking within different compartments of the secretory and endosomal pathway. Centaurin alpha-1 binds PIP3 and filamentous (F)-actin, and interacts with the clathrin coat adaptor AP-3. Centaurin a-1 has a conserved GAP homology domain and has been shown to decrease Arf6 GTP levels in vivo. Other studies from our lab show that centaurin alpha-1 is required for normal dendritic branching and spine morphogenesis in developing neurons. My preliminary studies demonstrate centaurin alpha-1 has a punctate staining pattern in neurosecretory cells and neurons and colocalizes with protein markers of the regulated secretory pathway. These data support the hypothesis that centaurin alpha-1 is an Arf6 GAP involved in secretory vesicle trafficking and is required in dendritic differentiation. Centaurin gamma-2 (also called AGAP1) shows Arf1 GAP activity in vitro and in vivo. Centaurin gamma-2 binds PIP3 and AP-3, and overexpression in non-neuronal cells results in transferrin accumulation in the recycling endosome. Centaurin gamma-2 was also identified as a candidate autism susceptibility gene. My preliminary data demonstrate centaurin gamma-2 overexpression inhibits differentiation of developing hippocampal neurons. These data support the hypothesis that centaurin gamma-2 is an Arf1 GAP that regulates neuronal morphology through its control of recycling endosomal trafficking. The goal of this dissertation is to make fundamental contributions to the understanding of how centaurins are involved in vesicle trafficking. This dissertation will address two specific aims: 1) Identify the role of centaurin alpha-1 in secretory vesicle trafficking, and 2) Understand the role of centaurin gamma-2 in endosomal trafficking and characterize its function in neuronal differentiation.

1 online resource (xi, 167 p. : ill., digital, PDF file)

Neurobiology;

Joint Health Sciences;

centaurin alpha-1 centaurin gamma-2 Arf vesicle trafficking dendritic differentiation

UNRESTRICTED

Advisors/Committee Members: Theibert, Anne, Collawn, Jim<br>, Pozzo-Miller, Lucas<br>, Sztul, Elizabeth<br>, Wilson, Scott.

Subjects/Keywords: Adaptor Proteins, Signal Transducing  – metabolism<; br>; Dendrites  – metabolism<; br>; Dentrites  – ultrastructure<; br>; GTPase-Activating Proteins  – metabolism<; br>; Hippocampus  – cytology<; br>; Neurons  – metabolism

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Larimore, J. L. (2008). The role of centaurins in vesicular trafficking and neuronal differentiation. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,823

Chicago Manual of Style (16th Edition):

Larimore, Jennifer Lynn. “The role of centaurins in vesicular trafficking and neuronal differentiation.” 2008. Doctoral Dissertation, University of Alabama – Birmingham. Accessed October 20, 2019. http://contentdm.mhsl.uab.edu/u?/etd,823.

MLA Handbook (7th Edition):

Larimore, Jennifer Lynn. “The role of centaurins in vesicular trafficking and neuronal differentiation.” 2008. Web. 20 Oct 2019.

Vancouver:

Larimore JL. The role of centaurins in vesicular trafficking and neuronal differentiation. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2008. [cited 2019 Oct 20]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,823.

Council of Science Editors:

Larimore JL. The role of centaurins in vesicular trafficking and neuronal differentiation. [Doctoral Dissertation]. University of Alabama – Birmingham; 2008. Available from: http://contentdm.mhsl.uab.edu/u?/etd,823

.