subject:(DNA vaccine)

University of Pretoria

1. [No author]. Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines .

Degree: 2010, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-02172010-170526/

► Four 1H12 E. ruminantium open reading frames cloned into the pCMViUBs mammalian expression vector and used as a recombinant DNA vaccine against heartwater repeatedly provided… (more)

▼ Four 1H12 E. ruminantium open reading frames cloned into the pCMViUBs mammalian expression vector and used as a recombinant DNA vaccine against heartwater repeatedly provided complete protection in sheep (using a cocktail or the individual ORFs) against a laboratory needle challenge while 1/5 of sheep were protected after a natural tick challenge. The lack of protection under natural field conditions could be attributed to the delivery strategy used and therefore there is a need to investigate other delivery methods. Polymeric microparticles based on PLGA polymers have been used extensively to target the delivery of vaccine to antigen presenting cells, play a role in the induction of cellular immunity and can be used as a single dose vaccine mimicking prime/boost vaccination. In this study, the four 1H12 pCMViUBs_ORFs and their respective recombinant proteins were either encapsulated into or adsorbed onto microparticles using a modified double emulsion solvent evaporation technique. The particles were formulated to release DNA on day zero and day 21 and recombinant proteins on day 42 thus mimicking a two times DNA prime/recombinant protein-boost immunization strategy. Encapsulation did not have any detrimental effects on the stability of the recombinant proteins as determined by gel electrophoresis and western blotting. The in vitro incubation of microparticles in either a Float-A-Lyzer® dialyzer or an eppendorf tube showed the potential of microparticles to be used as a vaccine because of their release profiles that mimics a heterologous prime/boost immunization strategy. Microparticles formulated using polymers with low glycolide ratios released 80% of the encapsulated proteins within the first week of in vitro incubation with most of the proteins released on day 1. Microparticles formulated using polymers with 50:50 monomer ratios released the recombinant proteins during week 1 and 3 of in vitro incubation. These microparticles did not release any protein in week 2 (day 7-14). Microparticles with 0.5% cetyltrimethylammonium bromide (CTAB) on their surfaces adsorbed DNA and released more than 40% of DNA on day 1 with 100% release by day 14. RG502H microparticles formed with PVA as the internal phase viscosity enhancer released intact DNA only from day 12 to day 21. A cocktail of these microparticles could therefore be used as an autobooster vaccine thus reducing the need for repeated immunizations needed to obtain protective immunity. Potential scientific publication Tshikhudo, N.P., Pretorius, A., Putterill, J., and van Kleef, M. 2009, “Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines”, Journal of Controlled Release, (draft manuscript). Publication of results in conference proceedings / abstracts NanoAfrica 2009: Biodegradable microspheres as a single dose delivery system for Ehrlichia ruminantium vaccines: N. Tshikhudo, A. Pretorius, J. Putterill and M. van Kleef. Advisors/Committee Members: Dr M van Kleef (advisor), Dr A Pretorius (advisor).

Subjects/Keywords: Ehrlichia ruminantium; DNA vaccine; UCTD

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APA (6^th Edition):

author], [. (2010). Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-02172010-170526/

Chicago Manual of Style (16^th Edition):

author], [No. “Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines .” 2010. Masters Thesis, University of Pretoria. Accessed January 15, 2021. http://upetd.up.ac.za/thesis/available/etd-02172010-170526/.

MLA Handbook (7^th Edition):

author], [No. “Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines .” 2010. Web. 15 Jan 2021.

Vancouver:

author] [. Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines . [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2021 Jan 15]. Available from: http://upetd.up.ac.za/thesis/available/etd-02172010-170526/.

Council of Science Editors:

author] [. Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines . [Masters Thesis]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-02172010-170526/

Universidade Nova

2. Amaral, Maria Rita Rebocho Lopes do. Evaluation of a new vaccine based on pDNA and recombinant protein against Helicobacter pylori.

Degree: 2012, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8520

►

Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

Helicobacter pylori is a bacterium capable of surviving and infecting a healthy human… (more)

▼

Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

Helicobacter pylori is a bacterium capable of surviving and infecting a healthy human stomach and it is estimated that infect more than a half of world population. Despite of being almost always asymptomatic, in some cases, the infection can evolve to several gastric disease as chronic gastritis, peptic ulcers, gastric cancer and MALT lymphoma. Vaccination against H. pylori is a promising option due to emerging problems of antibiotics treatment. It is thought that oral immunization could be a good approach for a more effective protection against infections by H. pylori, creating a first line of defense in mucosal surfaces. Chitosan nanoparticles are a suitable vehicle for oral vaccines delivery due to its immunogenic and mucoadhesive properties, protecting the DNA and allowing high levels of transfected cells. Thus, this work aims to evaluate a new pDNA- and recombinant protein-based vaccine, with multi epitopes of different H. pylori antigens. Following production and purification of plasmid DNA and recombinant proteins, vaccines were formulated for oral and intramuscular administration with the antigens encapsulated with chitosan nanoparticles. The type of immune response induced and the effectiveness of protective immunity elicited were assessed by ELISA, through analysis of specific IgGs, mucosal SIgA and cytokines levels produced by immunized BALB/C mice. When give by the intramuscular route, the formulated pDNA and recombinant protein-based vaccines efficiently stimulated the production of specific IgG2a and IgG1, which is supported by cytokines levels, revealing a better and balanced systemic immune response than oral immunizations. Nevertheless as expected, oral immunizations with either pDNA vaccines or recombinant protein revealed high levels of SIgA, showing to be effective in gastric mucosal immunization for a more protective immune response, contrasting with intramuscular immunizations which did not induce SIgA. The immunization results showed that both pDNA and recombinant proteins vaccines encapsulated with chitosan nanoparticles are good candidates for the development of a future vaccine to prophylactic and therapeutic use to improve the eradication of H. pylori infections.

Fundação da Ciência e Tecnologia - (PTDC/BIO/69242/2006); FEDER - (PEst-OE/SAU/UI4013/2011)

Advisors/Committee Members: Gonçalves, Lídia, Almeida, António.

Subjects/Keywords: Helicobacter pylori; Vaccine; DNA vaccine; Immunization; Recombinant antigens

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APA (6^th Edition):

Amaral, M. R. R. L. d. (2012). Evaluation of a new vaccine based on pDNA and recombinant protein against Helicobacter pylori. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8520

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amaral, Maria Rita Rebocho Lopes do. “Evaluation of a new vaccine based on pDNA and recombinant protein against Helicobacter pylori.” 2012. Thesis, Universidade Nova. Accessed January 15, 2021. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8520.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amaral, Maria Rita Rebocho Lopes do. “Evaluation of a new vaccine based on pDNA and recombinant protein against Helicobacter pylori.” 2012. Web. 15 Jan 2021.

Vancouver:

Amaral MRRLd. Evaluation of a new vaccine based on pDNA and recombinant protein against Helicobacter pylori. [Internet] [Thesis]. Universidade Nova; 2012. [cited 2021 Jan 15]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8520.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amaral MRRLd. Evaluation of a new vaccine based on pDNA and recombinant protein against Helicobacter pylori. [Thesis]. Universidade Nova; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8520

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

3. McGee, Michael. Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines.

Degree: MS, Animal Sciences, 2017, Louisiana State University

URL: etd-04102017-085926 ; https://digitalcommons.lsu.edu/gradschool_theses/4397

► Brucella is a genus of Gram negative, facultative intracellular pathogens which cause brucellosis, one of the most wide spread zoonotic diseases. Brucellosis causes a severe… (more)

Subjects/Keywords: vaccination; vaccine; Brucellosis; Brucella abortus; Brucella; DNA vaccine

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APA (6^th Edition):

McGee, M. (2017). Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines. (Masters Thesis). Louisiana State University. Retrieved from etd-04102017-085926 ; https://digitalcommons.lsu.edu/gradschool_theses/4397

Chicago Manual of Style (16^th Edition):

McGee, Michael. “Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines.” 2017. Masters Thesis, Louisiana State University. Accessed January 15, 2021. etd-04102017-085926 ; https://digitalcommons.lsu.edu/gradschool_theses/4397.

MLA Handbook (7^th Edition):

McGee, Michael. “Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines.” 2017. Web. 15 Jan 2021.

Vancouver:

McGee M. Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines. [Internet] [Masters thesis]. Louisiana State University; 2017. [cited 2021 Jan 15]. Available from: etd-04102017-085926 ; https://digitalcommons.lsu.edu/gradschool_theses/4397.

Council of Science Editors:

McGee M. Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines. [Masters Thesis]. Louisiana State University; 2017. Available from: etd-04102017-085926 ; https://digitalcommons.lsu.edu/gradschool_theses/4397

NSYSU

4. Yang, Ya-Ting. Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice.

Degree: Master, Biological Sciences, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0910112-163807

► The immunogenicity and protective efficacy of a DNA vaccine encoding a truncated groEL heat shock gene (pcDNA3/groEL) from Burkholderia pseudomallei was evaluated in vaccinated BALB/c… (more)

Subjects/Keywords: Melioidosis; heat shock proteins; DNA vaccine; Burkholderia

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APA (6^th Edition):

Yang, Y. (2012). Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0910112-163807

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Ya-Ting. “Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice.” 2012. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0910112-163807.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Ya-Ting. “Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice.” 2012. Web. 15 Jan 2021.

Vancouver:

Yang Y. Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice. [Internet] [Thesis]. NSYSU; 2012. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0910112-163807.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang Y. Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice. [Thesis]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0910112-163807

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

5. Palumbo, Rebecca Noelle. Improving polymer-mediated DNA vaccine delivery.

Degree: PhD, 2011, University of Minnesota

URL: http://purl.umn.edu/112726

► Vaccination using antigen-encoding plasmid DNA has great potential to generate strong immune response against delivered antigen. In order to effectively generate immune response, antigen must… (more)

Subjects/Keywords: Dendritic cells; DNA vaccine; Polyplex; Biomedical Engineering

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APA (6^th Edition):

Palumbo, R. N. (2011). Improving polymer-mediated DNA vaccine delivery. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/112726

Chicago Manual of Style (16^th Edition):

Palumbo, Rebecca Noelle. “Improving polymer-mediated DNA vaccine delivery.” 2011. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://purl.umn.edu/112726.

MLA Handbook (7^th Edition):

Palumbo, Rebecca Noelle. “Improving polymer-mediated DNA vaccine delivery.” 2011. Web. 15 Jan 2021.

Vancouver:

Palumbo RN. Improving polymer-mediated DNA vaccine delivery. [Internet] [Doctoral dissertation]. University of Minnesota; 2011. [cited 2021 Jan 15]. Available from: http://purl.umn.edu/112726.

Council of Science Editors:

Palumbo RN. Improving polymer-mediated DNA vaccine delivery. [Doctoral Dissertation]. University of Minnesota; 2011. Available from: http://purl.umn.edu/112726

University of Edinburgh

6. Luo, Honglin. Experimental DNA vaccine against filariasis.

Degree: PhD, 2011, University of Edinburgh

URL: http://hdl.handle.net/1842/5547

► Filarial infections are major causes of morbidity in the tropics and sub-tropics, afflicting over 150 million people in about 80 countries, causing debilitating symptoms such… (more)

▼ Filarial infections are major causes of morbidity in the tropics and sub-tropics, afflicting over 150 million people in about 80 countries, causing debilitating symptoms such as elephantiasis (lymphatic filariasis), dermatitis and blindness (onchocerciasis or river blindness). Current control of lymphatic filariais relies on mass drug treatment with diethylcarbamazine（DEC) and albendazole while ivermectin is used against onchocerciasis. Repeat treatment is frequently required and this highlights the possibility of development of drug resistance. In addition, risk of adverse reactions following treatment excludes some patients from control programmes. Such circumstances urgently call for the development of complementary control measures such as vaccination. DNA vaccines are novel type of subunit vaccine in which production of the immunizing antigen is induced in host cells after introduction of a plasmid or recombinant viral vector containing the gene that encodes the antigen. DNA vaccines are relatively simple and cheap to produce and their stability makes them particularly suitable for use in remote regions that lack the cold-chain storage facilities required of conventional vaccines. Filarial nematodes are tissue-dwelling parasites that survive for many years in immunocompetent hosts. It is proposed that this longevity is, in part, due to the capacity of the parasites to modulate potentially lethal Th2 responses of their hosts. Consequently, the efficiency of a filarial vaccine may depend on how well it circumvents filarial-driven immunosuppression. To test this hypothesis, a series of DNA vaccines were developed and tested in the Litomosoides sigmodontis-mouse model of filarial infections. The L. sigmodontis Abundant Larval Transcript-1 (Ls-ALT) and Cysteine Protease Inhibitor (Ls-CPI) genes were cloned and genetically engineered to ablate their immunomodulatory properties by deleting the acid domain and by site mutation, respectively. In addition, the L. sigmodontis Venom Allergen Homologue (Ls-VAH) and Thioredoxin Peroxidase (Ls-TPX) were used in their native forms as vaccine targets. To improve immunisation and antigen processing by the host, these parasite genes were fused to a DNA sequence encoding an antibody that specifically binds dendritic cell surface protein (αDEC205 single chain Fv). DNA plasmids carrying mutated forms and/or anti-DEC205 were then co-administered with plasmids encoding the Th2 promoting cytokine IL4, and/or antigen-presenting cell activating MIP1α and Flt3L. Mice immunized with mutated forms (ADDALT and CPImu) of parasite antigens produced more specific antibody post-challenge and showed strongly increased lymphocyte stimulation above controls immunized with the native form. The immune response was further enhanced when plasmids encoding IL4, MIP1α, Flt3L and anti-DEC-205 forms were co-administered, resulting in production of a Th2/IgG1 phenotype. Significant reduction of worm burden (82.3%) was achieved by a cocktail vaccination which combined the ADDALT and CPImu candidates. Mice…

Subjects/Keywords: 616.9883; DNA vaccine; Filariasis; Litomosoides sigmodontis

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APA (6^th Edition):

Luo, H. (2011). Experimental DNA vaccine against filariasis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5547

Chicago Manual of Style (16^th Edition):

Luo, Honglin. “Experimental DNA vaccine against filariasis.” 2011. Doctoral Dissertation, University of Edinburgh. Accessed January 15, 2021. http://hdl.handle.net/1842/5547.

MLA Handbook (7^th Edition):

Luo, Honglin. “Experimental DNA vaccine against filariasis.” 2011. Web. 15 Jan 2021.

Vancouver:

Luo H. Experimental DNA vaccine against filariasis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2011. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1842/5547.

Council of Science Editors:

Luo H. Experimental DNA vaccine against filariasis. [Doctoral Dissertation]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/5547

7. LIMA, Elyda Gonçalves de. Imunização genética para o controle de papilomaviroses: construção de um vetor vacinal baseado no Gene L2 do papilomavírus bovino tipo 1 .

Degree: 2011, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/18481

► A bovinocultura é um dos principais destaques do agronegócio brasileiro no cenário mundial. No entanto, algumas doenças vêm causando prejuízos consideráveis entre elas está a… (more)

Subjects/Keywords: Gene L2; Papilomavírus bovino; Vacina de DNA; Bovine papillomavirus; DNA vaccine

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APA (6^th Edition):

LIMA, E. G. d. (2011). Imunização genética para o controle de papilomaviroses: construção de um vetor vacinal baseado no Gene L2 do papilomavírus bovino tipo 1 . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/18481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

LIMA, Elyda Gonçalves de. “Imunização genética para o controle de papilomaviroses: construção de um vetor vacinal baseado no Gene L2 do papilomavírus bovino tipo 1 .” 2011. Thesis, Universidade Federal de Pernambuco. Accessed January 15, 2021. http://repositorio.ufpe.br/handle/123456789/18481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

LIMA, Elyda Gonçalves de. “Imunização genética para o controle de papilomaviroses: construção de um vetor vacinal baseado no Gene L2 do papilomavírus bovino tipo 1 .” 2011. Web. 15 Jan 2021.

Vancouver:

LIMA EGd. Imunização genética para o controle de papilomaviroses: construção de um vetor vacinal baseado no Gene L2 do papilomavírus bovino tipo 1 . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2011. [cited 2021 Jan 15]. Available from: http://repositorio.ufpe.br/handle/123456789/18481.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIMA EGd. Imunização genética para o controle de papilomaviroses: construção de um vetor vacinal baseado no Gene L2 do papilomavírus bovino tipo 1 . [Thesis]. Universidade Federal de Pernambuco; 2011. Available from: http://repositorio.ufpe.br/handle/123456789/18481

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tampere University

8. Malm, Maria. Assessing the Immunogenicity of GTU®-based HIV-1 Multigene DNA Vaccines in Murine Models .

Degree: 2011, Tampere University

URL: https://trepo.tuni.fi/handle/10024/66762

► Väitöskirjatyössä arvioitiin alun perin Tampereen yliopistossa ja myöhemmin FIT Biotech Oy:ssä kehitetyn HIV-1 rokotteen tehoa ja kykyä synnyttää HIV-spesifinen suojaava immuunivaste hiirimalleissa. Terapeuttisen HIV-1 rokotteen… (more)

▼ Väitöskirjatyössä arvioitiin alun perin Tampereen yliopistossa ja myöhemmin FIT Biotech Oy:ssä kehitetyn HIV-1 rokotteen tehoa ja kykyä synnyttää HIV-spesifinen suojaava immuunivaste hiirimalleissa. Terapeuttisen HIV-1 rokotteen tarkoituksena on herättää immuunipuolustus tuhoamaan elimistön HI-viruksen infektoimia soluja. Rokotteen osoitettiin tehokkaasti aktivoivan antigeenispesifisiä tappajasoluja ja herättävän suojaavan immuunivasteen kokeellisissa hiirimalleissa. Kehitetty geneettinen rokote, GTU®-MultiHIV, muodostaa multiantigeenin eli yhdistelmäproteiinin, joka koostuu HIV-1 Rev, Nef, Tat ja Gag p17/p24 -proteiineista ja T-solujen epitooppialueista. MultiHIV-proteiini tuotetaan elimistössä GTU® -vektorista, jonka on osoitettu muodostavan antigeeniä pidempikestoisesti ja tehokkaammin kuin perinteisten rokotevektoreiden. GTU®-MultiHIV immunisoinnin osoitettiin synnyttävän rokotespesifisen solu- ja vasta-ainevälitteisen immuunivasteen jokaista vektorin tuottamaa antigeeniä kohtaan. Immuunivasteen herättämisen osoitettiin olevan immunisaatioreitistä ja käytetystä annostuksesta riippuvainen, ihonsisäinen immunisointi todettiin lihaksensisäistä immunisaatiota tehokkaammaksi. Soluvälitteistä immuunivastetta arvioitiin immunisoitujen hiirien lymfosyyttien antigeenispesifisellä gamma interferonin erityksellä. HIV-spesifisen immuunivasteen suojatehoa arvioitiin kahdessa kokeellisessa hiirimallissa, joista ensimmäisessä mallissa arvioitiin immunisoinnin kykyä estää MultiHIV-antigeeniä kantavien kasvainsolujen lisääntymistä. Toisessa mallissa saatiin viitteitä rokotteen suojatehosta eri HIV-1 kantoja vastaan käyttämällä hiirien pseudovirusaltistukseen HIV-1 kantoja, jotka poikkesivat rokotteen HIV-1 antigeenien kannoista. Lopuksi tutkittiin dendriittisolujen oleellista merkitystä immuunivasteen syntymisessä GTU®-MultiHIV DNA immunisoinnin jälkeen uutta lähestymistapaa käyttäen.; HIV-1 vaccine development has proven an extremely challenging task, largely related to the highly variable nature of the virus which generates constantly new variants able to escape the immune system surveillance and lack of correlates of protection. DNA vaccines have the potential to encode multiple viral antigens, thereby eliciting immune responses that could lead to improved containment of the HIV-1 virus in a relatively safe way. Furthermore, the endogenous synthesis of the plasmid encoded antigen mimics the viral replication and enables the antigen presentation in a natural way for immune system cells. The first aim of this study was to evaluate the immunogenicity of the HIV-1 multigene DNA plasmid vaccine, encoding for Rev, Nef, Tat, p17, p24 and selected T cell epitopes of HIV-1 pol and env in mice. GTU® vector encoding the multigene is an advanced expression vector resulting in higher expression level and longer maintenance of the plasmid in dividing cells compared to conventional DNA plasmids. In the first part of the work, we demonstrated that GTU®-MultiHIV DNA induces cellular and humoral immune responses in mice directed to all…

Subjects/Keywords: HIV-1 ; plasmidi-DNA rokote ; hiirimalli ; DNA plasmid vaccine ; murine model

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Malm, M. (2011). Assessing the Immunogenicity of GTU®-based HIV-1 Multigene DNA Vaccines in Murine Models . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/66762

Chicago Manual of Style (16^th Edition):

Malm, Maria. “Assessing the Immunogenicity of GTU®-based HIV-1 Multigene DNA Vaccines in Murine Models .” 2011. Doctoral Dissertation, Tampere University. Accessed January 15, 2021. https://trepo.tuni.fi/handle/10024/66762.

MLA Handbook (7^th Edition):

Malm, Maria. “Assessing the Immunogenicity of GTU®-based HIV-1 Multigene DNA Vaccines in Murine Models .” 2011. Web. 15 Jan 2021.

Vancouver:

Malm M. Assessing the Immunogenicity of GTU®-based HIV-1 Multigene DNA Vaccines in Murine Models . [Internet] [Doctoral dissertation]. Tampere University; 2011. [cited 2021 Jan 15]. Available from: https://trepo.tuni.fi/handle/10024/66762.

Council of Science Editors:

Malm M. Assessing the Immunogenicity of GTU®-based HIV-1 Multigene DNA Vaccines in Murine Models . [Doctoral Dissertation]. Tampere University; 2011. Available from: https://trepo.tuni.fi/handle/10024/66762

Université Catholique de Louvain

9. Lambricht, Laure. Plasmids encoding viral structural proteins to enhance cancer DNA vaccine potency.

Degree: 2017, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/184680

►

Using the power of the immune system to prevent or destroy cancer is an attractive strategy. DNA vaccines are interesting candidates for this purpose, but… (more)

Subjects/Keywords: DNA vaccine; Electroporation; Cancer; Immunotherapy; Melanoma; Adjuvant; Tumour antigen; Vaccine delivery; Viral structural protein

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APA (6^th Edition):

Lambricht, L. (2017). Plasmids encoding viral structural proteins to enhance cancer DNA vaccine potency. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/184680

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lambricht, Laure. “Plasmids encoding viral structural proteins to enhance cancer DNA vaccine potency.” 2017. Thesis, Université Catholique de Louvain. Accessed January 15, 2021. http://hdl.handle.net/2078.1/184680.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lambricht, Laure. “Plasmids encoding viral structural proteins to enhance cancer DNA vaccine potency.” 2017. Web. 15 Jan 2021.

Vancouver:

Lambricht L. Plasmids encoding viral structural proteins to enhance cancer DNA vaccine potency. [Internet] [Thesis]. Université Catholique de Louvain; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2078.1/184680.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lambricht L. Plasmids encoding viral structural proteins to enhance cancer DNA vaccine potency. [Thesis]. Université Catholique de Louvain; 2017. Available from: http://hdl.handle.net/2078.1/184680

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade de Brasília

10. Alice Melo Ribeiro. Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica.

Degree: 2008, Universidade de Brasília

URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3733

►

As proteínas de choque térmico (HSPs) são reconhecidas como importantes moléculas na modulação do sistema imunológico e são altamente conservadas entre os diferentes organismos. A… (more)

▼

As proteínas de choque térmico (HSPs) são reconhecidas como importantes moléculas na modulação do sistema imunológico e são altamente conservadas entre os diferentes organismos. A vacina DNA-hsp65 de Mycobacterium leprae demonstrou efeitos profiláticos e imunoterapêuticos contra diversas doenças como, por exemplo, tuberculose, artrite e leishmaniose. Neste trabalho, avaliamos a eficácia e o potencial imunomodulador na imunização e no tratamento de camundongos infectados experimentalmente com o fungo patogênico Paracoccidioides brasiliensis, o agente etiológico da Paracoccidioidomicose, a mais importante micose endêmica na América Latina. A vacina DNA-hsp65 conferiu proteção aos animais contra o P. brasiliensis em ensaios de imunização ou de tratamento como indicado por: redução significativa no pulmonar fúngica carga (UFCs); diminuição da perda da função pulmonar e na presença de colágeno em granulomas (revelada por análises histológicas do tecido pulmonar); reestabelecimento proliferativo das células de baço; perfil de resposta ?, TNF-a e IGg2a e baixos níveis de IL-4, IL-10 imunológica de padrão Th1 com níveis elevados de IL-12, IFN - ? e IgG1. Esses dados, em conjunto, indicam que, em BALB/c, tanto a imunização como o tratamento com a vacina DNA-hsp65 conferem proteção no curso da Paracoccidioidomicose. Nossos resultados abrem novas perspectivas sobre a prevenção e tratamento para outras micoses sistêmicas.

Heat shock proteins (HSPs) are recognized as important molecules in the modulation of the immune system and are highly conserved among different organisms. The DNA-hsp65 vaccine from Mycobacterium leprae (M. leprae) has been shown to have prophylactic and immunotherapeutic effects against various diseases, for instance, tuberculosis, arthritis and leishmaniasis. In this work, we evaluated the effectiveness and immunomodulatory potential of the DNA-hsp65 immunization and treatment in model BALB/c mice infected with Paracoccidioides brasiliensis (P. brasiliensis), the etiological agent of Paracoccidioidomycosis, the most important endemic mycosis in Latin America. The DNA-hsp65 vaccine conferred protection against this pathogen for both, the prophylactic and therapeutic assays, as indicated by: a significant reduction in pulmonary fungal burden (CFUs); a decrease in pulmonary function loss and in the presence of collagen in granulomas (revealed by histological analyses of the pulmonary tissue); the reestablishment of spleen cells proliferation; the Th1 pattern a and IGg2a and lower levels of IL-4, IL-10 and immune response with higher levels of IL-12, IFN- ?, TNF-IgG1. Together, these findings indicate that, in mice, the immunization and treatment with the DNA-hsp65 vaccine protect mice against Paracoccidioidomycosis. Our results open new perspectives on prevention and treatment of other systemic mycoses.

Advisors/Committee Members: Gustavo Adolfo Sierra Romero, Maria Sueli Soares Felipe, Fernando Araripe Gonçalves Torres, Andréa Queiroz Maranhão, Carlos Pelleschi Taborda, Anamélia Lorenzetti Bocca, Ildinete Silva Pereira.

Subjects/Keywords: DNA vaccine; vacina de DNA; DNA-hsp65; CIENCIAS DA SAUDE; Paracoccidioides brasiliensis; Paracoccidioides brasiliensis; DNA-hsp65

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ribeiro, A. M. (2008). Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3733

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ribeiro, Alice Melo. “Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica.” 2008. Thesis, Universidade de Brasília. Accessed January 15, 2021. http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3733.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ribeiro, Alice Melo. “Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica.” 2008. Web. 15 Jan 2021.

Vancouver:

Ribeiro AM. Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica. [Internet] [Thesis]. Universidade de Brasília; 2008. [cited 2021 Jan 15]. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3733.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ribeiro AM. Vacina de DNA (hsp65 M. Leprae) para Paracoccidioidomicose Experimental: atividade imunogênica e terapêutica. [Thesis]. Universidade de Brasília; 2008. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3733

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

11. Liao, Jing-fong. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.

Degree: Master, Institute of Medical Science and Technology, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

► This study developed a flexible and rapidly-dissolvable microneedle (MN) patch for transdermal delivery and stability enhancement of DNA vaccine. The MN patch is made by… (more)

Subjects/Keywords: Transcutaneous immunization; Dissolving microneedle; DNA vaccine; Flexible patches; Polyvinyl alcohol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liao, J. (2016). Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liao, Jing-fong. “Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.” 2016. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liao, Jing-fong. “Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.” 2016. Web. 15 Jan 2021.

Vancouver:

Liao J. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liao J. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

12. Rijswoud, B. van. Canine Malignant Melanoma; The effectiveness of a new DNA vaccine.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/289056

► Introduction: Canine Malignant Melanoma (CMM) is a very aggressive, potential metastatic neoplasm of melanocytes. They account for 9 to 20% of all skin tumors and… (more)

▼ Introduction: Canine Malignant Melanoma (CMM) is a very aggressive, potential metastatic neoplasm of melanocytes. They account for 9 to 20% of all skin tumors and occur in dogs that have heavily pigmented skin or oral mucosa. CMM have a high chance of metastasis (51%) even after surgical removal of the primary tumor and adjuvant therapy. The mean survival time for dogs with CMM is 5 to 8 months. Xenogeneic DNA vaccination of dogs with CMM induce an immune response and may increase the survival time significantly. Aim of study: The aim of this study was to investigate the effectiveness of the CMM vaccine. The hypothesis of the study was that the vaccine as a supplementary therapy for CMM would increase the survival of dogs with MM. Materials and methods: Eight dogs with an average age of 9.2 years old were treated with a xenogeneic human tyrosinase vaccine against CMM, after surgical removal of the tumor and radiation therapy. The vaccine was administered 4 times biweekly, and a vaccination boost was suggested to be given 6 months after the last vaccination. After therapy, the dogs visited the surgical oncology policlinic at Utrecht according to a preset protocol. Results: One dog had recurrence of the tumor, one dog had metastasis and one dog developed a recurrence and a metastasis.The mean survival time for these eight dogs was 109 days. There was no increase in survival time observed, compared to the survival times described in literature with conventional treatment. One WHO stage 3 dog had a survival time of 132 days end died at the end of the study. Discussion: Eight dogs diagnosed with CMM were treated with xenogeneic human tyrosinase after surgival removal and radiotherapy. Three dogs had recurrence and/or metastasis of the primary tumor. No increase in survival time was observed in this study and no side effects were observed among the dogs. An explanation for the outcome could be the short study period and the limited number of cases. Advisors/Committee Members: Kirpensteijn, J..

Subjects/Keywords: Canine Malignant Melanoma; poor prognosis; DNA melanoma vaccine; survival time

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rijswoud, B. v. (2014). Canine Malignant Melanoma; The effectiveness of a new DNA vaccine. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289056

Chicago Manual of Style (16^th Edition):

Rijswoud, B van. “Canine Malignant Melanoma; The effectiveness of a new DNA vaccine.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 15, 2021. http://dspace.library.uu.nl:8080/handle/1874/289056.

MLA Handbook (7^th Edition):

Rijswoud, B van. “Canine Malignant Melanoma; The effectiveness of a new DNA vaccine.” 2014. Web. 15 Jan 2021.

Vancouver:

Rijswoud Bv. Canine Malignant Melanoma; The effectiveness of a new DNA vaccine. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 15]. Available from: http://dspace.library.uu.nl:8080/handle/1874/289056.

Council of Science Editors:

Rijswoud Bv. Canine Malignant Melanoma; The effectiveness of a new DNA vaccine. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/289056

University of Pretoria

13. Tshikhudo, Ndavheleseni Phanuel. Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines.

Degree: Veterinary Tropical Diseases, 2010, University of Pretoria

URL: http://hdl.handle.net/2263/30068

► Four 1H12 E. ruminantium open reading frames cloned into the pCMViUBs mammalian expression vector and used as a recombinant DNA vaccine against heartwater repeatedly provided… (more)

▼ Four 1H12 E. ruminantium open reading frames cloned into the pCMViUBs mammalian expression vector and used as a recombinant DNA vaccine against heartwater repeatedly provided complete protection in sheep (using a cocktail or the individual ORFs) against a laboratory needle challenge while 1/5 of sheep were protected after a natural tick challenge. The lack of protection under natural field conditions could be attributed to the delivery strategy used and therefore there is a need to investigate other delivery methods. Polymeric microparticles based on PLGA polymers have been used extensively to target the delivery of vaccine to antigen presenting cells, play a role in the induction of cellular immunity and can be used as a single dose vaccine mimicking prime/boost vaccination. In this study, the four 1H12 pCMViUBs_ORFs and their respective recombinant proteins were either encapsulated into or adsorbed onto microparticles using a modified double emulsion solvent evaporation technique. The particles were formulated to release DNA on day zero and day 21 and recombinant proteins on day 42 thus mimicking a two times DNA prime/recombinant protein-boost immunization strategy. Encapsulation did not have any detrimental effects on the stability of the recombinant proteins as determined by gel electrophoresis and western blotting. The in vitro incubation of microparticles in either a Float-A-Lyzer® dialyzer or an eppendorf tube showed the potential of microparticles to be used as a vaccine because of their release profiles that mimics a heterologous prime/boost immunization strategy. Microparticles formulated using polymers with low glycolide ratios released 80% of the encapsulated proteins within the first week of in vitro incubation with most of the proteins released on day 1. Microparticles formulated using polymers with 50:50 monomer ratios released the recombinant proteins during week 1 and 3 of in vitro incubation. These microparticles did not release any protein in week 2 (day 7-14). Microparticles with 0.5% cetyltrimethylammonium bromide (CTAB) on their surfaces adsorbed DNA and released more than 40% of DNA on day 1 with 100% release by day 14. RG502H microparticles formed with PVA as the internal phase viscosity enhancer released intact DNA only from day 12 to day 21. A cocktail of these microparticles could therefore be used as an autobooster vaccine thus reducing the need for repeated immunizations needed to obtain protective immunity. Potential scientific publication Tshikhudo, N.P., Pretorius, A., Putterill, J., and van Kleef, M. 2009, “Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines”, Journal of Controlled Release, (draft manuscript). Publication of results in conference proceedings / abstracts NanoAfrica 2009: Biodegradable microspheres as a single dose delivery system for Ehrlichia ruminantium vaccines: N. Tshikhudo, A. Pretorius, J. Putterill and M. van Kleef. Advisors/Committee Members: Van Kleef, Mirinda (advisor), Pretorius, Alri (advisor).

Subjects/Keywords: Ehrlichia ruminantium; DNA vaccine; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tshikhudo, N. (2010). Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/30068

Chicago Manual of Style (16^th Edition):

Tshikhudo, Ndavheleseni. “Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines.” 2010. Masters Thesis, University of Pretoria. Accessed January 15, 2021. http://hdl.handle.net/2263/30068.

MLA Handbook (7^th Edition):

Tshikhudo, Ndavheleseni. “Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines.” 2010. Web. 15 Jan 2021.

Vancouver:

Tshikhudo N. Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines. [Internet] [Masters thesis]. University of Pretoria; 2010. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2263/30068.

Council of Science Editors:

Tshikhudo N. Biodegradable microparticles as a single dose delivery system for Ehrlichia ruminantium vaccines. [Masters Thesis]. University of Pretoria; 2010. Available from: http://hdl.handle.net/2263/30068

14. 우, 규진. The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus.

Degree: 2006, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/1447 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001017

► "Hantaan virus (HTNV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). More than 100,000 cases of HFRS are reported yearly, with a… (more)

▼ "Hantaan virus (HTNV) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). More than 100,000 cases of HFRS are reported yearly, with a mortality rate of between 2% and 10%. But, there is no effective and safe vaccine against HFRS. Even though neutralizing antibodies against the HTNV have been proven to be critical against viral infections, the cellular immune responses to HTNV are also assumed to be important for viral clearance. This study has examined the cellular and humoral immune responses against the HTNV nucleocapsid protein (NP) elicited by virus infection or DNA vaccination to investigate the immunogenicity of NP. To examine the cellular immune response against HTNV NP, C57BL/6 mice were injected with HTNV intraperitoneal. The NP-specific CD8+ T cell response was analyzed using a 51Cr-release assay, intracellular cytokine assay, enzyme-linked immunospot assay and tetramer binding assay against H-2Kb restricted CTL epitopes of NP (M6 and N1 peptide). Using these methods, it was found that HTNV infection elicited a strong NP-specific CD8+ T cell response at 8 days after infection, and several different methods to check the NP-specific CD8+ T cell response showed a perfect correlation among analyses. To examine the humoral immune response against HTNV NP, the NP-specific antibody response was analyzed using an enzyme linked immunosorbent assay (ELISA). HTNV infection elicited the NP-specific humoral immune response which was began at 4~5 days after infection. DNA vaccine has been shown to elicit both humoral and cellular immune responses, and confer protection against some viral, bacterial and parasitic pathogens. Therefore, DNA vaccine strategy was applied to HTNV in this study. Sindbis virus-based expression vector was carefully designed and constructed, in order to induce the transient high level expression of target gene. In the case of DNA vaccination by plasmid encoding nucleocapsid gene, a single dose injection of 100㎍ of plasmid DNA into quardriceps muscle of C57BL/6 mice induced a high level of humoral and cellular immune response. The NP-specific antibody response was elicited 2~4 weeks after immunization and maximized at 6~10 weeks and sustained for over 14 weeks. NP-specific CD8+ T cell response reached its peak 2~3 weeks after immunization. Even though NP-specific CD8+ T cell response after DNA vaccination was not strong as the HTNV infection, but the pattern of response was similar to that of HTNV infection. In a challenge test with the recombinant vaccinia virus expressing NP (rVV-HTNV-N), the rVV-HTNV-N titers in DNA vaccinated mice were decreased about 100 fold compared control mice. Even though challenge with rVV-HTNV-N in HTNV infected mice were perfectly protected, but DNA vaccination showed the partial protection. In conclusion, this study showed that (i) HTNV infection in C57BL/6 mice elicited the strong NP-specific CD8+ T cell response at 8 days after infection, (ii) DNA vaccination with plasmid encoding HTNV nucleocapsid gene also elicited the strong NP-specific… Advisors/Committee Members: 대학원 의학과, 200124292, 우, 규진.

Subjects/Keywords: Hantaan virus; DNA vaccine

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APA (6^th Edition):

우, �. (2006). The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/1447 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001017

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

우, 규진. “The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus.” 2006. Thesis, Ajou University. Accessed January 15, 2021. http://repository.ajou.ac.kr/handle/201003/1447 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001017.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

우, 규진. “The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus.” 2006. Web. 15 Jan 2021.

Vancouver:

우 �. The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus. [Internet] [Thesis]. Ajou University; 2006. [cited 2021 Jan 15]. Available from: http://repository.ajou.ac.kr/handle/201003/1447 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001017.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

우 �. The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus. [Thesis]. Ajou University; 2006. Available from: http://repository.ajou.ac.kr/handle/201003/1447 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001017

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

15. Dietz, Wynette. Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy.

Degree: PhD, Microbiology, Immunology and Cancer Biology, 2010, University of Minnesota

URL: http://hdl.handle.net/11299/191376

► Since cancer is the second most common cause of death in the United States, it is of great importance to pursue new and improved methods… (more)

▼ Since cancer is the second most common cause of death in the United States, it is of great importance to pursue new and improved methods for treating cancer. The goal of cancer immunotherapy is to exploit the specificity and longevity of immune responses through the use of vaccines to treat cancer. DNA vaccines have many advantages over protein and viral vaccine-based strategies including low cost, ease of production, flexibility and low toxicity. Plasmid DNA vaccines encoding tumor antigens can produce powerful anti-tumor immune responses in animal models, but clinical trials have shown only modest responses. This lack of clinical efficacy is thought to reflect the two major limitations of plasmid DNA vaccines: transient protein expression and low transfection efficiency. Transient protein expression is likely the result of gene silencing due to transcriptionally repressive chromatin within the plasmid backbone. To overcome this limitation, we removed the bacterial backbone sequences and produced a minicircle DNA consisting of the gene expression cassette with only a few bases of the bacterial backbone. This resulted in persistent protein expression, increased transfection efficiency and enhanced immunogenicity. In an effort to further enhance the transfection efficiency, we produced cationic carriers that bind plasmid DNA and protect it from degradation. The addition of these cationic carriers significantly increased transfection efficiency in vitro but has yet to show the same effect in vivo. Additionally, we administered these vaccines transdermally using a tattoo device and achieved rapid and potent immune responses. Our results suggest transdermal delivery of a minicircle DNA vaccine elicits potent antigen-specific immune responses, and as such, holds great promise for cancer therapy. Future work will include increasing transfection efficiency in vivo and increasing the immunogenicity of the vaccines through the use of adjuvants with the goal of producing feasible, efficacious DNA vaccines for cancer therapy.

Subjects/Keywords: DNA; Minicircle; Plasmid; Vaccine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dietz, W. (2010). Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191376

Chicago Manual of Style (16^th Edition):

Dietz, Wynette. “Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy.” 2010. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/191376.

MLA Handbook (7^th Edition):

Dietz, Wynette. “Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy.” 2010. Web. 15 Jan 2021.

Vancouver:

Dietz W. Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy. [Internet] [Doctoral dissertation]. University of Minnesota; 2010. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/191376.

Council of Science Editors:

Dietz W. Enhanced Efficacy of Plasmid DNA Vaccines for Cancer Therapy. [Doctoral Dissertation]. University of Minnesota; 2010. Available from: http://hdl.handle.net/11299/191376

University of Melbourne

16. Cross, Ryan N S. Investigating MYB in the context of mammary gland biology, transformation and as a therapeutic target in breast and colon cancer.

Degree: 2014, University of Melbourne

URL: http://hdl.handle.net/11343/54724

► Breast cancer is the second most common form of malignancy diagnosed in Australian women, imposing an enormous social and economic burden on society. If the… (more)

Subjects/Keywords: breast cancer; colon cancer; mammary gland stem cells; DNA vaccine; immunotherapy

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APA (6^th Edition):

Cross, R. N. S. (2014). Investigating MYB in the context of mammary gland biology, transformation and as a therapeutic target in breast and colon cancer. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/54724

Chicago Manual of Style (16^th Edition):

Cross, Ryan N S. “Investigating MYB in the context of mammary gland biology, transformation and as a therapeutic target in breast and colon cancer.” 2014. Doctoral Dissertation, University of Melbourne. Accessed January 15, 2021. http://hdl.handle.net/11343/54724.

MLA Handbook (7^th Edition):

Cross, Ryan N S. “Investigating MYB in the context of mammary gland biology, transformation and as a therapeutic target in breast and colon cancer.” 2014. Web. 15 Jan 2021.

Vancouver:

Cross RNS. Investigating MYB in the context of mammary gland biology, transformation and as a therapeutic target in breast and colon cancer. [Internet] [Doctoral dissertation]. University of Melbourne; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11343/54724.

Council of Science Editors:

Cross RNS. Investigating MYB in the context of mammary gland biology, transformation and as a therapeutic target in breast and colon cancer. [Doctoral Dissertation]. University of Melbourne; 2014. Available from: http://hdl.handle.net/11343/54724

Washington University in St. Louis

17. Kim, Sojung. Single chain MHC trimer-based DNA vaccines for pathogen protection.

Degree: PhD, Biology and Biomedical Sciences: Immunology, 2011, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/etd/598

► CD8+ T cells play a major role in controlling infection and disease progression in many infectious diseases. Upon infection, antigen-specific CD8+ T cells are generated… (more)

▼ CD8+ T cells play a major role in controlling infection and disease progression in many infectious diseases. Upon infection, antigen-specific CD8+ T cells are generated and mainly through their cytotoxic activity remove infected cells, therefore, pathogens. Ongoing research has identified antigenic epitopes in a vast number of pathogens and, using the identified epitopes, the induction of CD8+ T cell immune responses has been an important strategy for successful vaccines. However, most immunization approaches with class I binding peptides have failed to induce CD8+ T cell responses strong enough to prevent disease. This failure has been attributed to the lack of CD4+ T cell help and difficulty in maintaining a sufficient level of antigen presentation required for CD8+ T cell activation. To circumvent these limitations, we have developed fully assembled MHC molecules that can be expressed as membrane-bound proteins on the cell surface, termed single chain trimers: SCTs). SCTs are composed of an immunodominant peptide, β2m, and MHC I heavy chain covalently linked by 15-20 amino acid flexible linkers. Because SCTs are expressed as a single polypeptide chain, they do not require peptide processing, or chaperone-assisted peptide loading in the ER. Furthermore, antigen presentation by the SCT bypasses the need to compete with an extensive pool of endogenous peptides for peptide loading. SCTs are folded properly and T cells see SCTs comparably to native peptide/MHC I complexes. Various human and mouse class Ia and Ib MHC molecules have been engineered with epitope peptides into SCTs and proven as useful tools to monitor and modulate immune responses. Thus, SCT engineering offers a great potential as a platform for antigen-specific DNA vaccines. Although there have been several reports of SCT-based DNA vaccines generating antigen-specific CTL responses, there have been no reports of pathogen protection after DNA vaccine expression of SCTs. In this thesis, I examined the efficacy of SCT DNA vaccines for the first time in pathogen infection models. First, we developed a clinically relevant human HLA-A2 transgenic mouse model of West Nile virus: WNV) infection and demonstrated protective efficacy of a HLA-A2 SCT DNA vaccine against lethal viral infection. Second, I validated the potency of a SCT DNA vaccine using the BALB/c model of Listeria monocytogenes infection, which indicated that SCT DNA vaccines also provide protective immunity against bacterial infection. Lastly, I demonstrated that further engineering of SCTs: dtSCT) using a disulfide trap to better accommodate epitopes can potentiate the capacity of SCTs to stimulate CD8+ T cells, suggesting broad application of SCTs even with low immunogenic peptides. I also used disulfide trap- or chimeric SCTs to test the mechanism of antigen presentation after DNA vaccination. My studies showed that SCTs are presented to T cells as intact molecules after DNA immunization, suggesting direct presentation by transfected DCs or cross-dressing as a major mechanism of the antigen… Advisors/Committee Members: Ted Hansen.

Subjects/Keywords: Immunology; DNA vaccine; pathogen infection; single chain MHC trimer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, S. (2011). Single chain MHC trimer-based DNA vaccines for pathogen protection. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/598

Chicago Manual of Style (16^th Edition):

Kim, Sojung. “Single chain MHC trimer-based DNA vaccines for pathogen protection.” 2011. Doctoral Dissertation, Washington University in St. Louis. Accessed January 15, 2021. https://openscholarship.wustl.edu/etd/598.

MLA Handbook (7^th Edition):

Kim, Sojung. “Single chain MHC trimer-based DNA vaccines for pathogen protection.” 2011. Web. 15 Jan 2021.

Vancouver:

Kim S. Single chain MHC trimer-based DNA vaccines for pathogen protection. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2011. [cited 2021 Jan 15]. Available from: https://openscholarship.wustl.edu/etd/598.

Council of Science Editors:

Kim S. Single chain MHC trimer-based DNA vaccines for pathogen protection. [Doctoral Dissertation]. Washington University in St. Louis; 2011. Available from: https://openscholarship.wustl.edu/etd/598

University of Adelaide

18. Masavuli, Makutiro Ghislain. Novel DNA Vaccine Formulations Against Hepatitis C Virus.

Degree: 2018, University of Adelaide

URL: http://hdl.handle.net/2440/127111

► No vaccines are available for hepatitis C virus (HCV) which infects over 71 million people worldwide. Current therapeutic options are very expensive and as a… (more)

▼ No vaccines are available for hepatitis C virus (HCV) which infects over 71 million people worldwide. Current therapeutic options are very expensive and as a consequence, only around 1% of those diagnosed with Hepatitis C receive treatment each year. Induction of neutralizing antibody (NAb) by vaccination will be important for the successful prevention of HCV infection. HCV envelope glycoproteins E1 and E2 are required for virus entry into host cells making these proteins attractive targets to prevent virus infection. DNA-based vaccines are appealing candidates for novel vaccine development because they are not infectious and are cost-effective to manufacture on a global scale. However, despite being licensed for veterinary use, DNA vaccine have not been highly immunogenic in large animals. Virus-like particles (VLP), on the other hand, which resemble native viruses but are non-infectious because they lack viral genetic materials, have provided highly encouraging results in clinical trials. In this thesis the immunogenicity of VLPs and the ease of production of plasmid DNA were combined by designing DNA vaccines encoding VLPs consisting of HCV-core, E1 and E2 proteins (which can self-assemble into VLPs following expression). This vaccine also encoded a cytolytic gene perforin (PRF) to cause cell death and ensure the release of the VLPs from vaccine-targeted cells along with damage associated molecular patterns (DAMPs), which act as natural adjuvants. Bicistronic DNA vaccine constructs encoding HCV structural proteins and PRF were successfully generated and validated. Vaccination with the DNA construct encoding CoreE1E2-PRF generated higher adaptive immune responses in mice than vaccination with a construct unable to induce cell death, therefore confirming an adjuvant effect by PRF. However, these responses were weak compared to those reported in the literature. Antigen oligomerisation has been shown to improve vaccine immunogenicity. To improve the immunogenicity of a DNA vaccine encoding HCV-E1E2, a novel strategy that incorporates E1 and E2 into heptamers by fusion with the oligomerisation domain of a chimeric C4 binding protein (namely IMX3133 or IMX313P) was used. As the adjuvanticity of IMX313 or IMX313P requires efficient secretion of the oligomerised protein, the leader sequence of the tissue plasminogen activator (tPA) was introduced upstream of the E1 or E2 proteins (tE1 or tE2) from which the transmembrane domains were removed. The use of tE1 and tE2 proteins as separate immunogens or as a single tE1tE2 polyprotein when fused to IMX313 or IMX313P was assessed in vaccination studies in Balb/c mice using prime-boost intra-dermal DNA immunisations. Vaccination with the DNA construct encoding tE1/tE2 fused to IMX313 or IMX313P resulted in increased antibody and cell mediated immune (CMI) responses compared to the same dose of DNA without IMX313 or IMX313P, while fusion of tE1/tE2 to IMX313P resulted in the highest immune responses. DNA prime/E1E2 protein boost or DNA prime/HCV-VLP boost approaches were then… Advisors/Committee Members: Grubor-Bauk, Branka (advisor), Wijesundara, Danushka (advisor), Adelaide Medical School (school).

Subjects/Keywords: Hepatitis C virus; virus-like particles; DNA vaccine; oligomerisation; E1 and E2; IMX313P; VLPs; neutralising antibodies; prophylactic vaccine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Masavuli, M. G. (2018). Novel DNA Vaccine Formulations Against Hepatitis C Virus. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/127111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Masavuli, Makutiro Ghislain. “Novel DNA Vaccine Formulations Against Hepatitis C Virus.” 2018. Thesis, University of Adelaide. Accessed January 15, 2021. http://hdl.handle.net/2440/127111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Masavuli, Makutiro Ghislain. “Novel DNA Vaccine Formulations Against Hepatitis C Virus.” 2018. Web. 15 Jan 2021.

Vancouver:

Masavuli MG. Novel DNA Vaccine Formulations Against Hepatitis C Virus. [Internet] [Thesis]. University of Adelaide; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2440/127111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Masavuli MG. Novel DNA Vaccine Formulations Against Hepatitis C Virus. [Thesis]. University of Adelaide; 2018. Available from: http://hdl.handle.net/2440/127111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vrije Universiteit Amsterdam

19. Mollers, M. Monitoring the impact of HPV vaccination: pre- and early post-vaccination data .

Degree: 2014, Vrije Universiteit Amsterdam

URL: http://hdl.handle.net/1871/52709

Subjects/Keywords: HPV; vaccination; monitoring; vaccine effectiveness; serology; DNA; vaccine; VE

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mollers, M. (2014). Monitoring the impact of HPV vaccination: pre- and early post-vaccination data . (Doctoral Dissertation). Vrije Universiteit Amsterdam. Retrieved from http://hdl.handle.net/1871/52709

Chicago Manual of Style (16^th Edition):

Mollers, M. “Monitoring the impact of HPV vaccination: pre- and early post-vaccination data .” 2014. Doctoral Dissertation, Vrije Universiteit Amsterdam. Accessed January 15, 2021. http://hdl.handle.net/1871/52709.

MLA Handbook (7^th Edition):

Mollers, M. “Monitoring the impact of HPV vaccination: pre- and early post-vaccination data .” 2014. Web. 15 Jan 2021.

Vancouver:

Mollers M. Monitoring the impact of HPV vaccination: pre- and early post-vaccination data . [Internet] [Doctoral dissertation]. Vrije Universiteit Amsterdam; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1871/52709.

Council of Science Editors:

Mollers M. Monitoring the impact of HPV vaccination: pre- and early post-vaccination data . [Doctoral Dissertation]. Vrije Universiteit Amsterdam; 2014. Available from: http://hdl.handle.net/1871/52709

University of Adelaide

20. Mekonnen, Zelalem Addis. Pre-Clinical Evaluation of a Vaccination Strategy to Induce Liver Resident Memory T Cells against Hepatitis C Virus.

Degree: 2019, University of Adelaide

URL: http://hdl.handle.net/2440/119950

► Hepatitis C virus (HCV) is a significant contributor to the global burden of disease with at least 71 million individuals persistently infected with the virus.… (more)

▼ Hepatitis C virus (HCV) is a significant contributor to the global burden of disease with at least 71 million individuals persistently infected with the virus. Although effective anti-viral therapies are available, they are prohibitive in cost and at least 80% of infected individuals are undiagnosed for HCV making the development of an effective vaccine a crucial requirement to eliminate HCV infections. There is much conjecture about how to design an effective HCV vaccine, nonetheless, there is evidence to suggest that a vaccine which can elicit T cell immunity in the liver will be protective. Firstly, tissue-resident memory CD8⁺ T (TRM) cells have been reported to patrol the liver sinusoids for the lifespan of the individual and play vital roles in the elimination of malaria parasites (Plasmodium berghei) following invasion of hepatocytes. Secondly, CD4⁺ and CD8⁺ T cell-mediated immunity against non-structural (NS) proteins of HCV correlate with recovery from acute infections in patients. DNA and adeno-associated virus (AAV) have an excellent safety profile and have been effective in gene therapy and vaccinations in humans. Our laboratory has also shown that a DNA vaccine encoding HCV NS proteins and a cytolytic protein, perforin (PRF), is more effective than a canonical DNA vaccine in eliciting HCV-specific T cell responses against NS proteins of HCV. Thus, using a DNA vaccine encoding NS5B from HCV genotype 3a and PRF (pVAX-NS5B-PRF) and recombinant AAV (rAAV) encoding the vaccine antigen (rAAV-NS5B-2A-eGFP) vaccination regimens designed to elicit HCV-specific T cell mediated immunity in the liver were developed and evaluated in this thesis. The vaccination strategy involved intradermal (ID) vaccination of mice with pVAX-NS5B-PRF to prime T cells followed by intravenous (IV) vaccination with rAAV-NS5B-2A-eGFP to trap the primed cells. To achieve the aims of this thesis, an established fluorescent target array (FTA) technology was modified to identify immunodominant CD4⁺ T cell and CD8⁺ T cell epitopes of NS5B in vivo to thoroughly analyse NS5B-specific T cell responses in vaccinated mice in vivo. Based on the in vivo epitope mapping analysis, a NS5B451-459 tetramer was developed that was crucial for isolating NS5B-specific CD8⁺ T cells in vaccinated BALB/c mice and determining whether cells had differentiated into TRM (i.e. CD69⁺ CD62L⁻) cells in the liver. rAAV-NS5B-2A-eGFP capable of transducing hepatocytes in vivo was also developed and used in vaccination studies. The vaccination regimen described in this thesis elicited robust NS5B-specific killing responses by CD8⁺ T cells and helper CD4⁺ T cell responses. Importantly, the heightened immunogenicity of the vaccination regimen also correlated with the formation of NS5B-specific CD8⁺ TRM cells in the liver. Although, the protective efficacy of this regimen still needs to be evaluated following an authentic HCV challenge, this study highlights that the prime/trap regimen is a promising strategy that can be used to develop a protective vaccination regimen against… Advisors/Committee Members: Gowans, Eric James (advisor), Gruber-Bauk, Branka (advisor), Wijesundara, Danushka Kumara (advisor), Adelaide Medical School (school).

Subjects/Keywords: Hepatitis C virus vaccine; DNA vaccine; adeno-associated virus vaccine; liver immunity; cytotoxic T cells; helper T cells; tissue resident memory cell

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mekonnen, Z. A. (2019). Pre-Clinical Evaluation of a Vaccination Strategy to Induce Liver Resident Memory T Cells against Hepatitis C Virus. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/119950

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mekonnen, Zelalem Addis. “Pre-Clinical Evaluation of a Vaccination Strategy to Induce Liver Resident Memory T Cells against Hepatitis C Virus.” 2019. Thesis, University of Adelaide. Accessed January 15, 2021. http://hdl.handle.net/2440/119950.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mekonnen, Zelalem Addis. “Pre-Clinical Evaluation of a Vaccination Strategy to Induce Liver Resident Memory T Cells against Hepatitis C Virus.” 2019. Web. 15 Jan 2021.

Vancouver:

Mekonnen ZA. Pre-Clinical Evaluation of a Vaccination Strategy to Induce Liver Resident Memory T Cells against Hepatitis C Virus. [Internet] [Thesis]. University of Adelaide; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2440/119950.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mekonnen ZA. Pre-Clinical Evaluation of a Vaccination Strategy to Induce Liver Resident Memory T Cells against Hepatitis C Virus. [Thesis]. University of Adelaide; 2019. Available from: http://hdl.handle.net/2440/119950

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

21. Wang, Xuting. Biochemical and immunological characterization of the immobilization antigens of Ichthyophthirius multifiliis.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/20471

► The pathogenic ciliate Ichthyophthirius multifiliis (Fouquet) infects a wide range of freshwater fish and causes the disease Ichthyophthiriasis (Ich) or “white spot”. Fish that survive… (more)

▼ The pathogenic ciliate Ichthyophthirius multifiliis (Fouquet) infects a wide range of freshwater fish and causes the disease Ichthyophthiriasis (Ich) or “white spot”. Fish that survive infection acquire immunity to subsequent challenge and produce specific serum antibodies that immobilize the parasite in vitro. Cell surface protein antigens targeted by these antibodies are referred to as immobilization antigens (i-antigens). Using immobilizing mouse monoclonal antibodies as ligands, the i-antigens of I. multifiliis were purified by immunoaffinity chromatography. The biochemical characteristics of purified i-antigens were studied with SDS-PAGE, MALDI-TOF, Western blotting, and Edman degradation, and the immunogenicity of purified i-antigens were confirmed by injecting rabbit and channel catfish (Ictalurus punctatus) to produce immobilizing antisera. Subunit vaccines comprised of the purified i-antigens induced protective immunity in juvenile channel catfish when administered with Freund’s adjuvant or a CpG oligodeoxynucleotide. Moreover, i-antigen subunit vaccines elicited immobilizing antibodies and conferred protection only against homologous parasites, which supports a model for immunity mediated by immobilizing antibodies. The i-antigen genes were introduced into a novel expression system, the free-living non-pathogenic ciliate Tetrahymena thermophila. The recombinant i-antigens were either targeted to the host cell surface in full-length form or secreted into the culture medium in a truncated form lacking the GPI-anchor addition site. The recombinant i-antigens had the same antigenicity and immunogenicity as i-antigens produced by the parasite. Live cells of Tetrahymena over-expressing Ichthyophthirius i-antigens were effective vaccines against I. multifiliis. DNA vaccines containing the i-antigen genes elicited serum antibody response and conferred protective immunity against I. multifiliis. These results clearly indicate that the i-antigens of I. multifiliis are protective antigens and good candidates for the development of vaccines against I. multifiliis.

Subjects/Keywords: Ichthyophthirius multifiliis; channel catfish (Ictalurus punctatus); immobilization antigen; immunization; subunit vaccine; recombinant vaccine; DNA vaccine; Freund\'s adjuvant; CpG oligodeoxynucleotide; Tetrahymena thermophila.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, X. (2014). Biochemical and immunological characterization of the immobilization antigens of Ichthyophthirius multifiliis. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/20471

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Xuting. “Biochemical and immunological characterization of the immobilization antigens of Ichthyophthirius multifiliis.” 2014. Thesis, University of Georgia. Accessed January 15, 2021. http://hdl.handle.net/10724/20471.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Xuting. “Biochemical and immunological characterization of the immobilization antigens of Ichthyophthirius multifiliis.” 2014. Web. 15 Jan 2021.

Vancouver:

Wang X. Biochemical and immunological characterization of the immobilization antigens of Ichthyophthirius multifiliis. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10724/20471.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang X. Biochemical and immunological characterization of the immobilization antigens of Ichthyophthirius multifiliis. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/20471

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Vaz, Sónia Maria Rendeiro. Melhoramento de uma vacina multi-antigénica contra a Helicobacter pylori.

Degree: 2014, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/17442

►

A Helicobacter pylori é uma bactéria que coloniza o estômago de cerca de metade da população mundial, estando associada ao aumento do risco de desenvolvimento… (more)

▼

A Helicobacter pylori é uma bactéria que coloniza o estômago de cerca de metade da população mundial, estando associada ao aumento do risco de desenvolvimento de gastrites, úlceras gástricas e duodenais, linfomas associados às mucosas e adenocarcinomas gástricos. Os problemas emergentes da terapia atual, baseada em antibióticos, torna a vacina uma alternativa promissora. No entanto, a elevada variabilidade genética desta bactéria tem dificultado o desenvolvimento de vacinas eficientes. Uma vacina multi-antigénica poderá constituir uma estratégia para ultrapassar esta limitação e obter-se uma vacina que represente as estirpes existentes mundialmente. Este trabalho visa aperfeiçoar uma vacina baseada em DNA plasmídico e proteína recombinante, constituída por múltiplos epítopos de diferentes antigénios de H. pylori, e avaliar a capacidade desta superar a imuno-variabilidade existente entre diferentes estirpes. Para aperfeiçoar a vacina substituiu-se o epítopo da GroEL, devido à sua homologia com proteínas humanas, por um epítopo da NapA, devido à relevância deste fator de virulência na infecção crónica. Observou-se nos estudos de imunização em murganhos, com estas duas novas construções de vacinas (proteica e de DNA plasmídico), uma resposta imunitária. Em Western Blot observou-se que os anticorpos policlonais gerados durante estas imunizações reconheceram todos os extractos proteicos obtidos de 15 estirpes de H. pylori, provenientes de regiões geográficas muito diferentes e obtidas de pacientes com diversas patologias gástricas. Os resultados obtidos corroboram a potencialidade de uma vacina multi-antigénica para contornar a elevada imuno-variabilidade da H. pylori e por consequência este tipo de construção pode constituir a base de uma vacina futura anti-H. pylori.

Helicobacter pylori is a bacterium that colonizes the stomach about half the world’s population, being associated with increased risk of various diseases such gastritis, gastric and duodenal ulcers, mucosa-associated lymphoid tissue lymphomas and gastric adenocarcinomas. Emerging problems of the current therapy, based on antibiotics, makes the vaccine a promising alternative. However, the high genetic variability of this bacterium has hampered the development of efficient vaccines. A multi-antigenic vaccine could be a strategy to overcome this limitation and obtain a vaccine that represents globally the existing world strains. This work aims to improve an approach based on plasmid DNA and recombinant protein vaccine, consisting of multiple epitopes from different H. pylori antigens, and evaluate the ability to overcome the immune variability between different strains. To improve the vaccine was replaced the epitope of GroEL, due to its homology with human proteins, for a epitope of NapA, due to the relevance of this virulence factor in chronic infection. It was observed in the immunization studies in mice, with these two new constructions of vaccines (plasmid DNA and protein), an immune response. In Western blot we found that polyclonal antibodies…

Advisors/Committee Members: Calado, Cecília.

Subjects/Keywords: Helicobacter pylori; Seleção antigénios; Imuno-variabilidade; Vacina multi-antigénica; Vacina de DNA; Antigénios recombinantes; Antigen selection; Immune variability; Multi-antigen vaccine; DNA vaccine; Recombinant antigen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaz, S. M. R. (2014). Melhoramento de uma vacina multi-antigénica contra a Helicobacter pylori. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/17442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vaz, Sónia Maria Rendeiro. “Melhoramento de uma vacina multi-antigénica contra a Helicobacter pylori.” 2014. Thesis, RCAAP. Accessed January 15, 2021. http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/17442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vaz, Sónia Maria Rendeiro. “Melhoramento de uma vacina multi-antigénica contra a Helicobacter pylori.” 2014. Web. 15 Jan 2021.

Vancouver:

Vaz SMR. Melhoramento de uma vacina multi-antigénica contra a Helicobacter pylori. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Jan 15]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/17442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vaz SMR. Melhoramento de uma vacina multi-antigénica contra a Helicobacter pylori. [Thesis]. RCAAP; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ucp.pt:10400.14/17442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

RMIT University

23. Penumarthi, A. Utilising nanoparticles for DNA vaccine delivery.

Degree: 2017, RMIT University

URL: http://researchbank.rmit.edu.au/view/rmit:162229

► Vaccines have been vital candidate against infectious diseases, and over the past one hundred years have saved millions of lives. The main types of vaccines… (more)

▼ Vaccines have been vital candidate against infectious diseases, and over the past one hundred years have saved millions of lives. The main types of vaccines available to date are subunit, live attenuated, inactivated, conjugate, toxoid and recombinant DNA based vaccines (Tiwle 2014). While effective, each has their own inherent limitations. For example, subunit vaccines cannot induce long term immunity, whereas live, attenuated and inactivated vaccines can have a limited target range (Abhishek et al., 2016). High production costs make recombinant vector based vaccines unavailable to poor economies (Nascimento et al., 2012). Toxoid vaccines need multiple doses with adjuvants and also cause strong local reactions (Baxter 2007). Even though DNA vaccines have the disadvantage of being limited to protein antigens, they offer long term immunity inducing all arms of the immune responses (Khan 2013). Most DNA vaccines are effective in eliciting immune responses without any side effects. The main criterion for a successful DNA vaccine is to have an efficient delivery system which can deliver it safely to the target cells. There are several successful delivery systems available for DNA vaccines; however no standard system is in place. For vaccine trials and effective DNA vaccination, targeting antigen presenting cells would be important. There is an increasing demand for novel DNA vaccine delivery systems, mainly for the non-viral type as they are considered relatively safe. Therefore, in this proof of concept study two novel delivery systems 1) Solid Lipid Nanoparticles (SLNs) and 2) yeast transposon virus like particles (Ty-VLPs) were chosen to study their potential to carry DNA vaccines in vitro to dendritic cells using eGFP plasmid as the reporter plasmid. Positively charged SLNs were synthesised by modified solvent-emulsification method and conjugated with plasmid DNA to form complexes (DNA-SLN complexes). The integrity of these complexes was confirmed by various agarose gel based assays. The SLN/DNA complexes were transfected into DC2.4 cells and analysed by flow cytometry for GFP expression. It was shown that there is a 10-fold increase in the transfection rate using these complexes in DC2.4 cells over plasmid alone and is comparable to that mediated by lipofectamine. On the other hand, Ty-VLPs were purified from the recombinant yeast constructed and plasmid DNA conjugated with them. The transfection efficiency of these complexes were analysed in vitro and was shown to increase compared to plasmid alone. In comparison the SLN system was more efficient for plasmid DNA delivery than Ty-VLPs.

Subjects/Keywords: Fields of Research; DNA vaccine delivery; Non viral vector; Dendritic cell uptake; Nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Penumarthi, A. (2017). Utilising nanoparticles for DNA vaccine delivery. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:162229

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Penumarthi, A. “Utilising nanoparticles for DNA vaccine delivery.” 2017. Thesis, RMIT University. Accessed January 15, 2021. http://researchbank.rmit.edu.au/view/rmit:162229.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Penumarthi, A. “Utilising nanoparticles for DNA vaccine delivery.” 2017. Web. 15 Jan 2021.

Vancouver:

Penumarthi A. Utilising nanoparticles for DNA vaccine delivery. [Internet] [Thesis]. RMIT University; 2017. [cited 2021 Jan 15]. Available from: http://researchbank.rmit.edu.au/view/rmit:162229.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Penumarthi A. Utilising nanoparticles for DNA vaccine delivery. [Thesis]. RMIT University; 2017. Available from: http://researchbank.rmit.edu.au/view/rmit:162229

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Wayne State University

24. Cani, Andi. Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (tieg1).

Degree: MS, Biochemistry and Molecular Biology, 2012, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_theses/202

► Cancer immunotherapy has had limited clinical efficacy partly because regulatory T cells (Treg) suppress the immune response to tumor-associated antigens. Inducible regulatory T cells… (more)

Subjects/Keywords: Cancer; DNA Vaccine; Her-2; Immunotherapy; iTreg; TIEG1; Immunology and Infectious Disease; Molecular Biology; Oncology

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APA (6^th Edition):

Cani, A. (2012). Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (tieg1). (Masters Thesis). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_theses/202

Chicago Manual of Style (16^th Edition):

Cani, Andi. “Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (tieg1).” 2012. Masters Thesis, Wayne State University. Accessed January 15, 2021. https://digitalcommons.wayne.edu/oa_theses/202.

MLA Handbook (7^th Edition):

Cani, Andi. “Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (tieg1).” 2012. Web. 15 Jan 2021.

Vancouver:

Cani A. Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (tieg1). [Internet] [Masters thesis]. Wayne State University; 2012. [cited 2021 Jan 15]. Available from: https://digitalcommons.wayne.edu/oa_theses/202.

Council of Science Editors:

Cani A. Modulation Of Anti-Tumor Immune Response By Tgf-Β-Inducible Early Gene 1 (tieg1). [Masters Thesis]. Wayne State University; 2012. Available from: https://digitalcommons.wayne.edu/oa_theses/202

University of Adelaide

25. Trahair, Hugh Francis Llewellyn. Suicide genes in a novel vaccination strategy for hepatitis C.

Degree: 2012, University of Adelaide

URL: http://hdl.handle.net/2440/96472

► Hepatitis C virus (HCV) is a blood transmitted virus which causes persistent infection of the liver greatly resulting in cirrhosis and hepatocellular carcinoma. Current treatment… (more)

▼ Hepatitis C virus (HCV) is a blood transmitted virus which causes persistent infection of the liver greatly resulting in cirrhosis and hepatocellular carcinoma. Current treatment regimens for HCV, such as pegylated interferon-α and ribavirin in combination, fail to clear the virus in 45-80% of individuals. New therapies under development, including immunotherapies, face many challenges. One of which is the need to induce a broad, multifunctional T-cell response directed against NS3/4A (cell-mediated immunity (CMI)), which is associated with clearance of infection, to enhance the immune response in chronic non-responders to conventional treatment. These individuals tolerate high levels of circulating HCV antigens and fail to clear the virus normally. Dendritic cells are the only antigen presenting cells able to prime naive T-cells, the central aim of any vaccination strategy. DC detect viral infection by phagocytosis of exogenous viral antigens released from infected, often apoptotic/necrotic cells, process and present these in association with MHC II to CD4+ T-cells and to naive CD8+ T-cells in association with MHC I (cross-presentation). Cross-presentation is proposed to be required for immunity to viruses, as immunity would otherwise only occur if the virus infected the DC (if direct presentation were the only possible mechanism). Therefore, one potential way to enhance the CMI is to enhance cross-presentation through induction of apoptosis or necrosis in vaccine-targeted cells. The overall aim of this study was to immunise C57BL/6 mice with DNA vaccines encoding HCV immunogens and genes encoding toxic proteins termed “suicide genes”. Induction of apoptosis/necrosis in the vaccine targeted cells was predicted to release the vaccine-derived antigen and enhance cross-presentation. This thesis explored suicide gene function in vitro and testing in vivo to examine the effect on the immunogenicity of a DNA vaccine strategy. Initially, suicide genes from a range of bacterial and viral sources were screened in vitro by luciferase co-transfection experiments to determine cell-killing ability. A highly toxic gene, diphtheria toxin subunit A (DTA), moderately toxic gene, mouse mutant perforin (MMP) and poorly toxic gene, rotavirus non-structural protein 4 (NSP4) were selected and each was cloned into a dual-promoter vector, then used to transfect HEK293T and Cos-7 cells. Alamar blue and additional luciferase experiments confirmed the cytotoxic effects. Finally, the mode of cell death was determined as apoptosis for DTA, necrosis for MMP and apoptosis and necrosis for NSP4 by lactate dehydrogenase (LDH) assay and Annexin V/Propidium iodide flow cytometry. DTA was later confirmed to inhibit protein synthesis and thus was not pursued in vivo. The HCV NS3 and NS3/4A serine protease genes were cloned under a second promoter in each dual-promoter suicide gene construct and protein expression was detected by western blot analysis. These DNA vaccines were used to immunise C57BL/6 mice to compare the immunogenicity by IFN-γ… Advisors/Committee Members: Gowans, Eric James (advisor), Miller, Darren Scott (advisor), Grubor-Bauk, Branka (advisor), School of Molecular and Biomedical Science (school).

Subjects/Keywords: suicide genes; DNA vaccine; HCV; hepatitis C; cross presentation; CMI T-cells; necrosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Trahair, H. F. L. (2012). Suicide genes in a novel vaccination strategy for hepatitis C. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/96472

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Trahair, Hugh Francis Llewellyn. “Suicide genes in a novel vaccination strategy for hepatitis C.” 2012. Thesis, University of Adelaide. Accessed January 15, 2021. http://hdl.handle.net/2440/96472.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Trahair, Hugh Francis Llewellyn. “Suicide genes in a novel vaccination strategy for hepatitis C.” 2012. Web. 15 Jan 2021.

Vancouver:

Trahair HFL. Suicide genes in a novel vaccination strategy for hepatitis C. [Internet] [Thesis]. University of Adelaide; 2012. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2440/96472.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trahair HFL. Suicide genes in a novel vaccination strategy for hepatitis C. [Thesis]. University of Adelaide; 2012. Available from: http://hdl.handle.net/2440/96472

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Victoria

26. Pinto, Crystal Tina. Temperature sensitive Mycobacterium tuberculosis as a potential vaccine candidate.

Degree: Department of Biochemistry and Microbiology, 2015, University of Victoria

URL: http://hdl.handle.net/1828/6280

► Mycobacterium tuberculosis remains one of the most common worldwide causes of illness and death due to an infectious disease. The emergence of multiple and extreme-drug… (more)

▼ Mycobacterium tuberculosis remains one of the most common worldwide causes of illness and death due to an infectious disease. The emergence of multiple and extreme-drug resistant strains has increased the need to find an effective vaccine for tuberculosis. The goal of our research group is to engineer a temperature-sensitive (TS) M. tuberculosis strain that can be used as a tool in vaccine development. One approach to create TS M. tuberculosis involves the integration of the essential gene ligA encoding a TS NAD+ dependent DNA ligase, which was taken from the psychrophilic organism Pseudoalteromonas haloplanktis. The integration and functioning of ligA was demonstrated in the fast-growing organism Mycobacterium smegmatis. This strain had a TS phenotype with growth limited to below 37°C. The strain was found to have a stable TS phenotype and did not mutate to a temperature-resistant form at a detectable level. Following experiments with the fast growing M. smegmatis, the integration of the ligA gene was attempted in slow-growing M. tuberculosis. Merodiploids of M. tuberculosis containing both the psychrophilic and the WT ligA gene in its chromosome were obtained. The second approach used for the development of TS M. tuberculosis was the directed evolution of native M. tuberculosis essential genes. An advantage of this approach is that the gene encoding the essential protein will resemble the native M. tuberculosis gene and thus will closely match the native transcriptional and translational rates. A system to screen and select for TS essential genes engineered by directed evolution was designed, where the essential gene on the chromosome of E. coli was knocked out and this gene was supplied on a conditionally replicating plasmid. As a first step in developing this directed evolution approach, a family of conditionally replicating plasmids were created and tested in an essential gene knock-out strain of E. coli. Advisors/Committee Members: Nano, Francis E. (supervisor).

Subjects/Keywords: Mycobacterium tuberculosis; vaccine; temperature-sensitive; directed evolution; psychrophiles; Tuberculosis; NAD+ dependent DNA ligase

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APA (6^th Edition):

Pinto, C. T. (2015). Temperature sensitive Mycobacterium tuberculosis as a potential vaccine candidate. (Masters Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/6280

Chicago Manual of Style (16^th Edition):

Pinto, Crystal Tina. “Temperature sensitive Mycobacterium tuberculosis as a potential vaccine candidate.” 2015. Masters Thesis, University of Victoria. Accessed January 15, 2021. http://hdl.handle.net/1828/6280.

MLA Handbook (7^th Edition):

Pinto, Crystal Tina. “Temperature sensitive Mycobacterium tuberculosis as a potential vaccine candidate.” 2015. Web. 15 Jan 2021.

Vancouver:

Pinto CT. Temperature sensitive Mycobacterium tuberculosis as a potential vaccine candidate. [Internet] [Masters thesis]. University of Victoria; 2015. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1828/6280.

Council of Science Editors:

Pinto CT. Temperature sensitive Mycobacterium tuberculosis as a potential vaccine candidate. [Masters Thesis]. University of Victoria; 2015. Available from: http://hdl.handle.net/1828/6280

27. Skinner, Nicole Elizabeth. Strategies to improve gene expression and targeting for DNA vaccine development.

Degree: PhD, Microbiology, Immunology and Cancer Biology, 2013, University of Minnesota

URL: http://purl.umn.edu/161088

► This dissertation examines strategies for improving DNA vaccines. Despite their many advantages and the considerable promise shown in small animal models, poor immunogenicity resulting from… (more)

Subjects/Keywords: DNA; Gene delivery; Minicircle; Vaccine

…situ by a DNA vaccine when they demonstrated that pDNA encoding human growth hormone… …both unique and particularly valuable among vaccine modalities. Due to the promise of DNA… …stimulants that can be encoded in the DNA vaccine. These genetic adjuvants include cytokines… …interest. For instance, as mentioned above, DNA vaccine-encoded Ags need to be presented by APCs… …study the use of MC DNA as a vaccine. In chapter 2, a MC DNA vaccine is shown to generate…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Skinner, N. E. (2013). Strategies to improve gene expression and targeting for DNA vaccine development. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/161088

Chicago Manual of Style (16^th Edition):

Skinner, Nicole Elizabeth. “Strategies to improve gene expression and targeting for DNA vaccine development.” 2013. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://purl.umn.edu/161088.

MLA Handbook (7^th Edition):

Skinner, Nicole Elizabeth. “Strategies to improve gene expression and targeting for DNA vaccine development.” 2013. Web. 15 Jan 2021.

Vancouver:

Skinner NE. Strategies to improve gene expression and targeting for DNA vaccine development. [Internet] [Doctoral dissertation]. University of Minnesota; 2013. [cited 2021 Jan 15]. Available from: http://purl.umn.edu/161088.

Council of Science Editors:

Skinner NE. Strategies to improve gene expression and targeting for DNA vaccine development. [Doctoral Dissertation]. University of Minnesota; 2013. Available from: http://purl.umn.edu/161088

University of Melbourne

28. Subair, Muhammath Siyam. Fertility control of common brushtail possums, Trichosurus vulpecula, in free-ranging populations.

Degree: 2011, University of Melbourne

URL: http://hdl.handle.net/11343/36543

► The common brushtail possum, Trichosurus vulpecula, is a serious ecological and agricultural pest in New Zealand, where it was introduced, and is very abundant in… (more)

▼ The common brushtail possum, Trichosurus vulpecula, is a serious ecological and agricultural pest in New Zealand, where it was introduced, and is very abundant in many Australian cities. Management of overabundant native species is contentious, with wildlife managers increasingly using fertility control as a humane option. My study developed three DNA vaccines based on coat protein 4 (CP4) and vesicle associated protein 1 (VAP1), previously shown to reduce fertility by ca 40% for a mean period of 1.4 years and 1.5 years respectively. CP4 and VAP1 cDNA were amplified by PCR, separately cloned into pcDNA3.1 plasmid vector, transformed into Escherichia coli, confirmed by sequencing, purified from endotoxins, and verified by in vitro expression in mammalian cells before use. The DNA vaccines were pcDNA3.1-CP4, pcDNA3.1-VAP1, a combined vaccine of the two (pcDNA3.1-CP4 + pcDNA3.1-VAP1), and a control vaccine (pcDNA3.1 blank plasmid). The effect of vaccines on animal behaviour was tested both on captive and free-ranging possums. Compared to controls, immunization with CP4 or the combined vaccine resulted in significant behavioural changes related to social rank and number of dens used in captivity and home range areas, long-range movements and den types used in the wild. The combined vaccine, the most effective vaccine, caused the highest degree of changes, while VAP1, the weakest contraceptive produced the lowest degree of changes. The social rank increased significantly in pcDNA3.1-CP4 and combined vaccine-immunized possums compared to controls. However, the dominance behaviour was positively correlated with weight gain. The absence of weight gain in wild females in my study after treatment means that dominance patterns in free-ranging possums are unlikely to change as a result of this factor after vaccination. Similarly, while immunized females used a higher number of dens compared to controls in captivity, the number of dens used by free-ranging females did not change between groups following treatment. Both pcDNA3.1-CP4 and combined vaccine-immunized females undertook significantly higher proportion of long-range movements and had a larger home range area compared to controls. These movements were associated with the absence of dependent young. No evidence of dispersal or mortalities was found.

Subjects/Keywords: DNA vaccine; vertebrate pest management; gene cloning; life-science; environmental management; environmental pharmacology; ecology

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APA (6^th Edition):

Subair, M. S. (2011). Fertility control of common brushtail possums, Trichosurus vulpecula, in free-ranging populations. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/36543

Chicago Manual of Style (16^th Edition):

Subair, Muhammath Siyam. “Fertility control of common brushtail possums, Trichosurus vulpecula, in free-ranging populations.” 2011. Doctoral Dissertation, University of Melbourne. Accessed January 15, 2021. http://hdl.handle.net/11343/36543.

MLA Handbook (7^th Edition):

Subair, Muhammath Siyam. “Fertility control of common brushtail possums, Trichosurus vulpecula, in free-ranging populations.” 2011. Web. 15 Jan 2021.

Vancouver:

Subair MS. Fertility control of common brushtail possums, Trichosurus vulpecula, in free-ranging populations. [Internet] [Doctoral dissertation]. University of Melbourne; 2011. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11343/36543.

Council of Science Editors:

Subair MS. Fertility control of common brushtail possums, Trichosurus vulpecula, in free-ranging populations. [Doctoral Dissertation]. University of Melbourne; 2011. Available from: http://hdl.handle.net/11343/36543

Virginia Tech

29. Pasnik, David J. Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp.

Degree: MS, Veterinary Medical Sciences, 2002, Virginia Tech

URL: http://hdl.handle.net/10919/10157

► Recombinant and DNA vaccines utilizing Mycobacterium sp. antigen 85A (Ag85A) were assessed for immunostimulatory and protective effects against M. marinum. Because of their known susceptibility… (more)

▼ Recombinant and DNA vaccines utilizing Mycobacterium sp. antigen 85A (Ag85A) were assessed for immunostimulatory and protective effects against M. marinum. Because of their known susceptibility to piscine mycobacteriosis, Morone sp. were utilized as the models for these studies. The first study evaluated a recombinant vaccine with a Brucella abortus strain RB51 vector expressing the Mycobacterium bovis Ag85A. Striped bass (M. saxatilis) were inoculated at doses equivalent to 106, 107, 108, 109, and 1010 colony-forming units/fish. Vaccinated fish demonstrated significant specific humoral and cell-mediated immune responses towards the Ag85A in a dose-dependant manner. However, vaccinated fish failed to demonstrate cross-protective responses after live Mycobacterium marinum challenge 70 days post-vaccination. A DNA vaccine was constructed utilizing the Mycobacterium marinum Ag85A gene and a commercially-available eukaryotic expression vector. Hybrid striped bass (M. saxatilis x M. chrysops) were immunized by intramuscular (i.m.) and intraperitoneal (i.p.) injection at doses of 5 μg, 25 μg, or 50 μg plasmid. These fish produced significant Ag85A-specific antibody and lymphoproliferative responses over those of control fish injected with saline or empty plasmid. Non-specific macrophage phagocytic and respiratory burst functions failed to exhibit significant upregulation after vaccination. Fish receiving the DNA vaccine developed protective responses to high-dose M. marinum challenge 90 days post-vaccination, as demonstrated by increased relative percent survival and by reduced splenic bacterial counts over control fish. Furthermore specific immunostimulatory and protective effects were significantly increased using higher vaccine doses and using the i.m. injection route. Given these promising findings, the protective responses induced by the DNA vaccine were further investigated. Hybrid striped bass were injected with 25 μg or 50 μg plasmid i.m. and developed specific protective responses to high-dose M. marinum challenge 120 days post-vaccination. The 25 μg and 50 μg groups both developed more rapidly and significantly increased immune responses post-challenge over those of the control groups. The vaccination groups also demonstrated increased survival, reduced splenic bacterial counts, and reduced granuloma formation compared to the control groups. However, though the vaccination groups did not demonstrate the same acute effects post-challenge as the control groups, the vaccination groups ultimately developed increased splenic bacterial counts and granuloma formation, and eventually experienced 100% mortalities. Because piscine mycobacteriosis can affect virtually any species of fish, a vaccine against this disease could be widely beneficial to the aquaculture and ornamental fish industries. The vaccines in these studies exhibited significant immunostimulatory capabilities in Morone sp., but only the DNA vaccine showed promise for conferring protection against M. marinum challenge. Though the DNA vaccine only… Advisors/Committee Members: Smith, Stephen A. (committeechair), Schurig, Gerhardt G. (committee member), Sriranganathan, Nammalwar (committee member).

Subjects/Keywords: DNA; Morone; Vaccine; Mycobacterium; Fish; Immunity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pasnik, D. J. (2002). Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/10157

Chicago Manual of Style (16^th Edition):

Pasnik, David J. “Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp.” 2002. Masters Thesis, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/10157.

MLA Handbook (7^th Edition):

Pasnik, David J. “Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp.” 2002. Web. 15 Jan 2021.

Vancouver:

Pasnik DJ. Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp. [Internet] [Masters thesis]. Virginia Tech; 2002. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/10157.

Council of Science Editors:

Pasnik DJ. Immunologic and Protective Effects of Vaccines for Mycobacterium marinum in Morone sp. [Masters Thesis]. Virginia Tech; 2002. Available from: http://hdl.handle.net/10919/10157

Loyola University Chicago

30. Gambino, Frank, Jr. Induction of Zika Virus-Specific Cd8+ T Cells by Means of DNA Vaccine.

Degree: MS, Microbiology and Immunology, 2018, Loyola University Chicago

URL: https://ecommons.luc.edu/luc_theses/3674

► Zika virus (ZIKV) is a member of the virus family flaviviridae and is transmitted via Aedes aegypti mosquitos. Monoclonal antibodies against dengue virus have… (more)

Subjects/Keywords: DNA Vaccine; Immunity; Papilloma Pseudovirus; T cell; Zika Virus; Immunology and Infectious Disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gambino, Frank, J. (2018). Induction of Zika Virus-Specific Cd8+ T Cells by Means of DNA Vaccine. (Thesis). Loyola University Chicago. Retrieved from https://ecommons.luc.edu/luc_theses/3674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gambino, Frank, Jr. “Induction of Zika Virus-Specific Cd8+ T Cells by Means of DNA Vaccine.” 2018. Thesis, Loyola University Chicago. Accessed January 15, 2021. https://ecommons.luc.edu/luc_theses/3674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gambino, Frank, Jr. “Induction of Zika Virus-Specific Cd8+ T Cells by Means of DNA Vaccine.” 2018. Web. 15 Jan 2021.

Vancouver:

Gambino, Frank J. Induction of Zika Virus-Specific Cd8+ T Cells by Means of DNA Vaccine. [Internet] [Thesis]. Loyola University Chicago; 2018. [cited 2021 Jan 15]. Available from: https://ecommons.luc.edu/luc_theses/3674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gambino, Frank J. Induction of Zika Virus-Specific Cd8+ T Cells by Means of DNA Vaccine. [Thesis]. Loyola University Chicago; 2018. Available from: https://ecommons.luc.edu/luc_theses/3674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations