subject:(DNA polymeraasi gamma )

Tampere University

1. Nurminen, Anssi. A Bioinformatics Approach to Analyzing the Pathogenicity of Mutations by Using Protein Structure Information : A Study on DNA Polymerase Gamma .

Degree: 2018, Tampere University

URL: https://trepo.tuni.fi/handle/10024/102657

► Geneettisen testauksen kustannusten laskiessa, pullonkaulaksi saatavilla olevan tiedon hyödyntämiseen on muodostumassa tietämyksemme eri geneettisten variaatioiden ja mutaatioden merkityksestä. Jokaisella henkilöllä on satoja, yksilöllisiä variaatioita perimässään… (more)

▼ Geneettisen testauksen kustannusten laskiessa, pullonkaulaksi saatavilla olevan tiedon hyödyntämiseen on muodostumassa tietämyksemme eri geneettisten variaatioiden ja mutaatioden merkityksestä. Jokaisella henkilöllä on satoja, yksilöllisiä variaatioita perimässään joiden vaikutuksia ei tunneta. Tilanteissa joissa perinöllisen sairauden syytä pyritään selvittämään geenitutkimuksen avulla nämä variaatiot pitää pystyä tunnistamaan joko merkityksettömiksi tai merkitysellisiksi taudin kannalta, jotta oikean diagnoosin tekeminen ja sopivien hoitometelmien valinta on mahdollista. Rakennebiologia ja bioinformatiikka tarjoavat monia keinoja mutaatioiden vaikutusten tarkasteluun. Tässä tutkimuksessa käytetään esimerkkinä DNA polymeraasi gammaa (Pol γ), joka on ainoa tunnettu entsyymi joka replikoi ja ylläpitää mitokondrionaalista DNA:ta. Pol γ tarjoaa uniikin tutkimuskohteen pistemutaatioiden vaikutuksille sen kriittisen toiminnalisuuden ja korvaavien mekanismien puuttumisen vuoksi. Pol γ:n tunnetun proteiinirakenteen, yli 700 hengen potilasaineiston ja mutaatioiden biokemiallisen karakterisoinnin avulla pystymme tuottamaan ennennäkemättömän tarkan kuvan mutaatioiden vaikutusmekanismeista ja ennustamaan sekä tunnettujen, että vielä tuntemattomien mutaatioiden vaikutusta taudin puhkeamiseen ja etenemiseen. Tämän tutkimuksen osana olemme kehittäneet uuden algoritmin proteiinien kolmiulotteisten rakenteiden tutkimukseen ja analysointiin, nimeltään StructureMapper. StructureMapper on skaalautuva, suurien aineistojen analysoitiin suunniteltu algoritmi, joka tarjoaa mahdollisuuksia analysoida esimerkikisi ennustusalgoritmien tulosten luotettavuutta, kokeellisen datan laatua, ja sitä voidaan hyödyntää myös mutaatioiden haitallisuuden ennustamisessa ja tutkimuksessa. Kaikkia tämän tutkimuksen osana tuotetuista menetelmistä ja työkaluista voidaan hyödyntää yleiskäyttöisesti proteiinien tutkimuksessa.; Genetic testing is becoming more and more prevalent in the field of medicine. The bottleneck in making a diagnosis based on genetic information has shifted from being able to acquire the required information by sequencing the genome of a patient, to understanding the effects of the detected, unique variations in the DNA. Mutations in the DNA can affect proteins and their structure on many levels. In a clinical setting, being able to separate the benign mutations from the pathogenic is of utmost importance. DNA polymerase gamma (Pol γ) offers an intriguing study subject in the world of structural biology. It is the sole enzyme responsible for replicating mitochondrial DNA. Defects in its functionality can manifest with a wide variety of symptoms that are often due to single point mutations that affect its structure in a deleterious way. Through analysis of the structure of Pol γ, combined with over 700 available patient case reports and biochemical characterization of some of the mutations, we have gained an unprecedented view to the reasons behind the mutations that lead to pathogenicity and altered biological functionality of…

Subjects/Keywords: DNA polymeraasi gamma ; POLG ; mutaatio ; proteiinirakenne ; patogeenisyys ; DNA polymerase gamma ; mutation ; protein structure ; pathogenicity

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nurminen, A. (2018). A Bioinformatics Approach to Analyzing the Pathogenicity of Mutations by Using Protein Structure Information : A Study on DNA Polymerase Gamma . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/102657

Chicago Manual of Style (16^th Edition):

Nurminen, Anssi. “A Bioinformatics Approach to Analyzing the Pathogenicity of Mutations by Using Protein Structure Information : A Study on DNA Polymerase Gamma .” 2018. Doctoral Dissertation, Tampere University. Accessed March 03, 2021. https://trepo.tuni.fi/handle/10024/102657.

MLA Handbook (7^th Edition):

Nurminen, Anssi. “A Bioinformatics Approach to Analyzing the Pathogenicity of Mutations by Using Protein Structure Information : A Study on DNA Polymerase Gamma .” 2018. Web. 03 Mar 2021.

Vancouver:

Nurminen A. A Bioinformatics Approach to Analyzing the Pathogenicity of Mutations by Using Protein Structure Information : A Study on DNA Polymerase Gamma . [Internet] [Doctoral dissertation]. Tampere University; 2018. [cited 2021 Mar 03]. Available from: https://trepo.tuni.fi/handle/10024/102657.

Council of Science Editors:

Nurminen A. A Bioinformatics Approach to Analyzing the Pathogenicity of Mutations by Using Protein Structure Information : A Study on DNA Polymerase Gamma . [Doctoral Dissertation]. Tampere University; 2018. Available from: https://trepo.tuni.fi/handle/10024/102657

Tampere University

2. Rovio, Anja. DNA Polymerase Gamma Mutations in Male Infertility and Ageing .

Degree: Lääketieteellisen teknologian instituutti - Institute of Medical Technology, 2006, Tampere University

URL: https://trepo.tuni.fi/handle/10024/67615

► Mitokondrio on solun energiaa tuottava organelli, jolla on oma genomi, mitokondriaalinen DNA (mtDNA). Mitokondrion DNA:ta monistaa yksi ainoa polymeraasi, polymeraasi gamma (POLG). Kirjallisuudessa on kuvattu… (more)

▼ Mitokondrio on solun energiaa tuottava organelli, jolla on oma genomi, mitokondriaalinen DNA (mtDNA). Mitokondrion DNA:ta monistaa yksi ainoa polymeraasi, polymeraasi gamma (POLG). Kirjallisuudessa on kuvattu jo noin 50 POLG mutaatiota eli geenivirhettä, ja jo pitkään niiden on uskottu olevan yhteydessä paitsi moniin hermosto- ja lihasrappeuma sairauksiin, myös vanhenemiseen liittyvien oireiden syntyyn. Tämän tutkimuksen ensimmäisessä osassa analysoitiin POLG:ssä olevan CAG-toistoalueen pituusvaihteluja eri väestöryhmissä, yleisimmissä mitokondriaalisissa sairauksissa ja lapsettomilla miehillä. Kaikissa tutkituissa väestöryhmissä toistoalueen pituus oli hyvin vakaa, yleisimmän alleelin sisältäessä 10 CAG-jaksoa. Myös kaikilla kädellisten ryhmillä on oma lajityypillinen, yleinen CAG-pituutensa, joka pitenee tultaessa pienistä apinoista simpanssin kautta gorillaan ja ihmiseen. Toistojakson pituus ei tavallisimmissa mitokondriaalisissa sairauksissa poikennut kontrolliväestöstä. Sen sijaan havaittiin, että noin 10%:lla lapsettomista miehistä puuttuu tämä ns. yleinen alleeli, kun taas tunnetusti fertiileillä miehillä se ei puuttunut yhdeltäkään. Näillä miehillä oli alentunut siittiöiden määrä, liikkuvuus oli huonontunut ja myös rakenteessa oli normaalia enemmän virheitä. Lapsettomilla miehillä havaittiin myös useita muita POLG mutaatioita, sekä kohonnut mtDNA:n mutaatioiden määrä. Muiden ryhmien tekemissä tutkimuksissa on havaittu, että näitä pariskuntia voidaan auttaa saamaan lapsi ICSI-tekniikalla, jossa siittiö viedään neulalla munasolun sisään. POLG toistoalueen pituutta kannattaisikin käyttää yhtenä kriteerinä hoitokeinoja mietittäessä niissä tapauksissa, joissa mitään muuta syytä lapsettomuuteen ei tiedetä. Tutkimuksen toinen osa keskittyi mtDNA:n mutaatioiden osuuteen vanhenemisessa. Vanhoilla ihmisillä on havaittu olevan enemmän mtDNA mutaatioita kuin nuorilla. Tämän uskotaan johtavan lisääntyneeseen happiradikaalien muodostumiseen solussa ja edelleen lisäävän mtDNA:n mutaatioiden määrää. Teoriaa testattiin Tukholmassa tehdyllä koemallilla, jossa POLG-entsyymissä oleva mtDNA:n korjausaktiivisuus turmeltiin mutaatiolla, ja tämä vioitettu "mutaattori" geeni vietiin hiireen. Mutaattorihiiret syntyivät normaaleina, mutta alkoivat jo puolen vuoden iässä vanheta näkyvästi. Niillä oli mm. osteoporoosia, kyttyräselkä, hiustenlähtöä, anemiaa, selkeää painonpudotusta, sydänsairauden ja hermoston rappeutumisen merkkejä. Sekä koirailla että naarailla oli enneaikaista hedelmättömyyttä. Tampereella tehdyissä geneettisissä analyyseissä todettiin, että hiirten kaikkiin kudoksiin kertyi moninkertaisesti enemmän mtDNA:n mutaatioita kuin samanikäisiin verrokkihiiriin. Tämä ilmeni soluhengityksen vajavaisuutena ja vähentyneenä energiantuottona kudoksissa. Hiirillä ei kuitenkaan ilmennyt selkeästi lisääntynyttä happiradikaalien tuotantoa. Hiirten keskimääräinen elinikä oli lyhentynyt, noin 48 viikkoa, kun se vastaavilla verrokkeilla oli noin 2,5 vuotta. Mutaattorihiiri mallina osoittaa, että suuri mtDNA:n mutaatioiden määrä johtaa…

Subjects/Keywords: polymeraasi gamma ; miehen lapsettomuus ; vanheneminen ; POLG ; male infertility ; ageing ; mtDNA mutations

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rovio, A. (2006). DNA Polymerase Gamma Mutations in Male Infertility and Ageing . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/67615

Chicago Manual of Style (16^th Edition):

Rovio, Anja. “DNA Polymerase Gamma Mutations in Male Infertility and Ageing .” 2006. Doctoral Dissertation, Tampere University. Accessed March 03, 2021. https://trepo.tuni.fi/handle/10024/67615.

MLA Handbook (7^th Edition):

Rovio, Anja. “DNA Polymerase Gamma Mutations in Male Infertility and Ageing .” 2006. Web. 03 Mar 2021.

Vancouver:

Rovio A. DNA Polymerase Gamma Mutations in Male Infertility and Ageing . [Internet] [Doctoral dissertation]. Tampere University; 2006. [cited 2021 Mar 03]. Available from: https://trepo.tuni.fi/handle/10024/67615.

Council of Science Editors:

Rovio A. DNA Polymerase Gamma Mutations in Male Infertility and Ageing . [Doctoral Dissertation]. Tampere University; 2006. Available from: https://trepo.tuni.fi/handle/10024/67615

University of New South Wales

3. Gautam, Shweta Dutta. The sequence specificity of bleomycin and gamma radiation-induced damage in systematically altered DNA sequences.

Degree: Biotechnology & Biomolecular Sciences, 2018, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/60202 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51297/SOURCE2?view=true

► Bleomycin is an anti-tumour agent that is clinically used to treat several types of cancers. Bleomycin cleaves DNA at specific DNA sequences and recent genome-wide… (more)

Subjects/Keywords: Gamma radiation; Bleomycin; DNA sequences

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gautam, S. D. (2018). The sequence specificity of bleomycin and gamma radiation-induced damage in systematically altered DNA sequences. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/60202 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51297/SOURCE2?view=true

Chicago Manual of Style (16^th Edition):

Gautam, Shweta Dutta. “The sequence specificity of bleomycin and gamma radiation-induced damage in systematically altered DNA sequences.” 2018. Doctoral Dissertation, University of New South Wales. Accessed March 03, 2021. http://handle.unsw.edu.au/1959.4/60202 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51297/SOURCE2?view=true.

MLA Handbook (7^th Edition):

Gautam, Shweta Dutta. “The sequence specificity of bleomycin and gamma radiation-induced damage in systematically altered DNA sequences.” 2018. Web. 03 Mar 2021.

Vancouver:

Gautam SD. The sequence specificity of bleomycin and gamma radiation-induced damage in systematically altered DNA sequences. [Internet] [Doctoral dissertation]. University of New South Wales; 2018. [cited 2021 Mar 03]. Available from: http://handle.unsw.edu.au/1959.4/60202 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51297/SOURCE2?view=true.

Council of Science Editors:

Gautam SD. The sequence specificity of bleomycin and gamma radiation-induced damage in systematically altered DNA sequences. [Doctoral Dissertation]. University of New South Wales; 2018. Available from: http://handle.unsw.edu.au/1959.4/60202 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51297/SOURCE2?view=true

4. Λογοθέτη, Στυλιανή. Μελέτη του ρόλου των p73 ισομορφών στον καρκίνο του πνεύμονα.

Degree: 2011, University of Crete (UOC); Πανεπιστήμιο Κρήτης

URL: http://hdl.handle.net/10442/hedi/24877

►

The p73 gene possesses an extrinsic P1 promoter and an intrinsic P2 promoter, resulting in synthesis of the full-length TAp73 and the N-terminal-truncated ΔNp73 isoforms,… (more)

▼

The p73 gene possesses an extrinsic P1 promoter and an intrinsic P2 promoter, resulting in synthesis of the full-length TAp73 and the N-terminal-truncated ΔNp73 isoforms, respectively. The ultimate effect of p73 in oncogenesis is thought to depend on the apoptotic TAp73 to the antiapoptotic ΔNp73 isoforms’ ratio. The selective activation of p73 promoters could trigger the expression of either the apoptotic or the antiapoptotic p73 isoforms, thus shifting the ΔN/ΤΑ ratio towards an oncogenic or an oncosuppression direction. Therefore, the epigenetic and transcription factors that differentially activate P1 and P2 promoters are crucial deterninants of the role of p73 in cancer, since they can alter the ΔN/ΤΑ ratio. This study was aimed at identifying novel epigenetic and transcription factors that affect both TAp73 and ΔNp73 isoform synthesis in the context of lung cancer, where both TAp73 and ΔNp73 isoforms have been previously found overexpressed. First, we investigated the DNA methylation status of both promoters as a means of epigenetic transcriptional control of their corresponding isoforms in 102 primary non-small cell lung carcinomas (NSCLCs). We demonstrated that while P1 hypermethylation-associated reduction of TAp73 mRNA levels is rare, the P2 hypomethylation-associated overexpression of ΔNp73 mRNA is a frequent event, particularly among squamous cell carcinomas. P1 promoter remained essentially unmethylated in the majority of lung cancer carcinomas. On the other hand, P2 hypomethylation strongly correlated with the hypomethylation of LINE-1 transposon, a marker of global DNA hypomethylation, indicating that ΔNp73 overexpression may be a passive consequence of global DNA hypomethylation. Since overexpression of TAp73 isoforms could not be attributed to change in the methylation status of P1 promoter, we plausibly hypothesized that it could potentially be caused by deregulated activity of specific transcription factor(s) . In the light of this notion, we then searched for novel transcription factors that could activate P1 promoter and result to TAp73 overexpression in lung cancer. With the use of bioinformatics tools, in vitro binding assays, and chromatin immunoprecipitation analysis, a region extending -233 to -204 bps upstream of the transcription start site of the human p73 P1 promoter, containing conserved Sp1 binding sites, was characterized. Treatment of cells with Sp1 RNAi and Sp1 inhibitor functionally suppresses TAp73 expression, indicating positive regulation of P1 by the Sp1 protein. Notably Sp1 inhibition or silencing also reduces ΔΝp73 protein levels. Therefore, Sp1 directly regulates TAp73 transcription and affects ΔΝp73 levels in lung cancer. TAp73γ was shown to be the only TA isoform overexpressed in several lung cancer cell lines and in 26 non-small cell lung cancers, consistent with Sp1 overexpression, thereby questioning the apoptotic role of this specific p73 isoform in lung cancer.

Το γονίδιο p73 διαθέτει έναν εξωτερικό υποκινητή (P1) και έναν εσωτερικό υποκινητή (P2), από τους οποίους…

Subjects/Keywords: Μεθυλίωση DNA; Καρκίνος πνεύμονα; ΔΝp73; p73; Sp1; DNA methylation; Lung cancer; TAp73 γ

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Λογοθέτη, . �. (2011). Μελέτη του ρόλου των p73 ισομορφών στον καρκίνο του πνεύμονα. (Thesis). University of Crete (UOC); Πανεπιστήμιο Κρήτης. Retrieved from http://hdl.handle.net/10442/hedi/24877

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Λογοθέτη, Στυλιανή. “Μελέτη του ρόλου των p73 ισομορφών στον καρκίνο του πνεύμονα.” 2011. Thesis, University of Crete (UOC); Πανεπιστήμιο Κρήτης. Accessed March 03, 2021. http://hdl.handle.net/10442/hedi/24877.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Λογοθέτη, Στυλιανή. “Μελέτη του ρόλου των p73 ισομορφών στον καρκίνο του πνεύμονα.” 2011. Web. 03 Mar 2021.

Vancouver:

Λογοθέτη �. Μελέτη του ρόλου των p73 ισομορφών στον καρκίνο του πνεύμονα. [Internet] [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10442/hedi/24877.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Λογοθέτη �. Μελέτη του ρόλου των p73 ισομορφών στον καρκίνο του πνεύμονα. [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2011. Available from: http://hdl.handle.net/10442/hedi/24877

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Meng, Qingchao, master of arts in cell and molecular biology. Approaching the crystal structure of the polymerase γ catalytic complex: Approaching the crystal structure of the polymerase [gamma] catalytic complex.

Degree: MA, Cell and Molecular Biology, 2011, University of Texas – Austin

URL: http://hdl.handle.net/2152/ETD-UT-2011-08-4330

► In this thesis, a 4.7Å crystal structure of the human mitochondria DNA polymerase γ catalytic complex is reported. Though the DNA substrate-binding site is not… (more)

Subjects/Keywords: Pol γ; Crystal structure; Catalytic complex; Polymerase γ; Pol gamma; Polymerase gamma; Human mitochondria DNA polymerase gamma; DNA; DNA binding site; Human mitochondria DNA polymerase γ

…17 viii Chapter 1: Introduction Unlike other DNA polymerases (DNAP), Pol γ… …mitochondrial DNA maintenance, repair, cell energy supply and viability. A defective Pol γ could cause… …the Pol γ-DNA structure, the distal monomer of the Pol γB subunit rotates towards the Pol γA… …that the movement of the thumb subdomain of Pol γ is a result of forming a DNA-bound complex… …Section of the electron density map. Electron density for part of the expected Pol γ-DNA…

9 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Meng, Qingchao, m. o. a. i. c. a. m. b. (2011). Approaching the crystal structure of the polymerase γ catalytic complex: Approaching the crystal structure of the polymerase [gamma] catalytic complex. (Masters Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2011-08-4330

Chicago Manual of Style (16^th Edition):

Meng, Qingchao, master of arts in cell and molecular biology. “Approaching the crystal structure of the polymerase γ catalytic complex: Approaching the crystal structure of the polymerase [gamma] catalytic complex.” 2011. Masters Thesis, University of Texas – Austin. Accessed March 03, 2021. http://hdl.handle.net/2152/ETD-UT-2011-08-4330.

MLA Handbook (7^th Edition):

Meng, Qingchao, master of arts in cell and molecular biology. “Approaching the crystal structure of the polymerase γ catalytic complex: Approaching the crystal structure of the polymerase [gamma] catalytic complex.” 2011. Web. 03 Mar 2021.

Vancouver:

Meng, Qingchao moaicamb. Approaching the crystal structure of the polymerase γ catalytic complex: Approaching the crystal structure of the polymerase [gamma] catalytic complex. [Internet] [Masters thesis]. University of Texas – Austin; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152/ETD-UT-2011-08-4330.

Council of Science Editors:

Meng, Qingchao moaicamb. Approaching the crystal structure of the polymerase γ catalytic complex: Approaching the crystal structure of the polymerase [gamma] catalytic complex. [Masters Thesis]. University of Texas – Austin; 2011. Available from: http://hdl.handle.net/2152/ETD-UT-2011-08-4330

6. Aubry, Valentin. Étude de la régulation de l'expression des gènes tardifs du virus d'Epstein-Barr : Study of the regulation of late Epstein-Barr virus genes expression.

Degree: Docteur es, Sciences de la Vie, 2016, Lyon

URL: http://www.theses.fr/2016LYSEN029

►

Le virus d’Epstein-Barr (EBV) est un Herpesvirus humain appartenant à la sous-famille des γ-Herpesvirinae. L’expression des gènes d’EBV est régulée très finement, de manière séquentielle… (more)

▼

Le virus d’Epstein-Barr (EBV) est un Herpesvirus humain appartenant à la sous-famille des γ-Herpesvirinae. L’expression des gènes d’EBV est régulée très finement, de manière séquentielle et débute par l’expression des gènes immédiats précoces dont les produits contrôlent l’expression des gènes précoces. Les produits de ces derniers permettent la réplication de l’ADN viral et la synthèse des gènes tardifs. Les mécanismes contrôlant l’expression des gènes immédiats précoces et précoces sont assez bien compris, à contrario de ceux qui contrôlent l’expression des gènes tardifs. En effet, il est connu que ces derniers ne sont exprimés qu’après réplication de l’ADN viral. Par ailleurs, les promoteurs des gènes tardifs présentent une structure différente des promoteurs de gènes immédiats précoces, précoces ou des gènes cellulaires. Ils sont essentiellement caractérisés par la présence d’une boîte TATT à la place de la TATA-box canonique. EBV a la particularité de coder pour une TBP-like, la protéine BcRF1 qui est essentielle mais pas suffisante pour l’expression des gènes viraux tardifs et qui se fixe spécifiquement sur la séquence TATT des promoteurs viraux tardifs. Notre objectif était principalement d’identifier l’ensemble des protéines virales nécessaires à l’expression des gènes tardifs d’EBV. Nous avons ainsi démontré qu’en plus de la protéine BcRF1, EBV code pour cinq autres protéines nécessaires à l’expression des gènes viraux tardifs : BFRF2, BGLF3, BVLF1, BDLF4 et BDLF3.5. Ces six protéines virales forment un complexe qui permet le recrutement de l’ARN polymérase II sur les promoteurs des gènes viraux tardifs. Nous avons nommé ce complexe vPIC (viral Pre-Initiation Complex), par analogie avec le complexe d’initiation de la transcription cellulaire formé autour de la TATA-Binding Protein (TBP). Le vPIC est conservé chez les herpesvirus des sous familles β et γ, mais est absent chez les herpesvirus de la sous famille des α. Par ailleurs, nos résultats suggèrent que le complexe vPIC interagit avec le complexe de réplication viral ce qui peut expliquer la liaison entre réplication de l’ADN viral et transcription des gènes viraux tardifs. Ces résultats permettent ainsi de mieux comprendre les mécanismes de régulation de l’expression des gènes tardifs d’EBV et serviront de base pour la recherche de nouveaux antiviraux.

The Epstein-Barr virus (EBV) is a human Herpersvirus belonging to the subfamily of γ-Herpesvirinae. EBV genes expression is tightly regulated, in a sequential manner and begins with the expression of immediate early genes whose products control the expression of early genes. Products of these allow the viral DNA synthesis and late genes expression. Mechanisms controlling immediate early and early genes expression are well documented, in contrast to those controlling late genes expression. Indeed, it is known that late genes are expressed after viral DNA replication. Furthermore, late genes promoters have a different structure of those of immediate early, early and cellular genes. They are essentially…

Advisors/Committee Members: Gruffat, Henri (thesis director).

Subjects/Keywords: Γ-Herpesvirinae; Gènes tardifs; Transcription; VPIC; Réplication de l’ADN viral; Γ-Herpesvirinae; Late genes; Transcription; VPIC; Viral DNA replication

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aubry, V. (2016). Étude de la régulation de l'expression des gènes tardifs du virus d'Epstein-Barr : Study of the regulation of late Epstein-Barr virus genes expression. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2016LYSEN029

Chicago Manual of Style (16^th Edition):

Aubry, Valentin. “Étude de la régulation de l'expression des gènes tardifs du virus d'Epstein-Barr : Study of the regulation of late Epstein-Barr virus genes expression.” 2016. Doctoral Dissertation, Lyon. Accessed March 03, 2021. http://www.theses.fr/2016LYSEN029.

MLA Handbook (7^th Edition):

Aubry, Valentin. “Étude de la régulation de l'expression des gènes tardifs du virus d'Epstein-Barr : Study of the regulation of late Epstein-Barr virus genes expression.” 2016. Web. 03 Mar 2021.

Vancouver:

Aubry V. Étude de la régulation de l'expression des gènes tardifs du virus d'Epstein-Barr : Study of the regulation of late Epstein-Barr virus genes expression. [Internet] [Doctoral dissertation]. Lyon; 2016. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2016LYSEN029.

Council of Science Editors:

Aubry V. Étude de la régulation de l'expression des gènes tardifs du virus d'Epstein-Barr : Study of the regulation of late Epstein-Barr virus genes expression. [Doctoral Dissertation]. Lyon; 2016. Available from: http://www.theses.fr/2016LYSEN029

7. vakrakou, aglaia. Μελέτη των μηχανισμών διαταραχής κάθαρσης αποπτωτικών και νευρωτικών κυττάρων στο Σύνδρομο Sjogren.

Degree: 2019, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/45772

► Primary Sjögren’s syndrome (SS) is a relatively common autoimmune disorder that is primarily characterized by chronic lymphoepithelial inflammation in the exocrine glands, mainly the salivary… (more)

▼ Primary Sjögren’s syndrome (SS) is a relatively common autoimmune disorder that is primarily characterized by chronic lymphoepithelial inflammation in the exocrine glands, mainly the salivary and lachrymal glands, leading to dryness. SS may extend from disease confined to the exocrine glands (organ-specific exocrinopathy) to various extraglandular manifestations (systemic disease) and the development of non-Hodgkin’s lymphoma (usually of mucosa-associated lymphoid tissue type; MALT-NHL) in 5% of patients. Approximately one-third of patients manifest clinico-laboratory features that represent high-risk prognostic factors for lymphoma development (type-I disease; as opposed to type-II disease, i.e. without adverse prognostic factors). Although the aetiopathogenesis of the disease remains unclear, evidence indicates the contribution of both innate and adaptive immunity in inflammatory reactions. In this PhD dissertation, we sought to investigate the innate immune responses elicited by tissue injury in SS patients.Apoptosis represents a major mechanism of programmed cell death that is essential for the regulation of tissue growth and homeostasis. Normally, cells dying by apoptosis undergo specific changes that target them for rapid clearance by professional phagocytes, such as macrophages. Typically, this process leads to the active production of anti-inflammatory mediators by phagocytes and thus facilitates the “immunologically silent” removal of apoptotic cells. The prompt elimination of apoptotic cells (also termed “efferocytosis”) is a very crucial biological process, since lingering apoptotic cells eventually proceed to the state of “late apoptosis” or “secondary necrosis” wherein they may contribute to inflammatory reactions via the release of immunogenic intracellular components, including modified autoantigens and “danger signals”. In fact, apoptosis and efferocytosis act in concert to regulate various processes, such as embryogenesis, tissue homeostasis, tolerance the elimination of damaged cells, and the resolution of inflammation.The occurrence of defective efferocytosis in certain inflammatory diseases is thought to have pathogenetic significance, based on the pro-inflammatory potential of secondary necrotic cells. Among them, systemic lupus erythematosus (SLE) is regarded as the archetypical disease model where the impaired clearance of apoptotic cells by macrophages represents a possible mechanism for the development of chronic autoimmune reactions and organ damage. Given the similarities (serological, immunogenetical and clinical) between SLE and SS, the important role of apoptosis in the pathogenesis of SS and the involvement of macrophages in the development of lymphoepithelial lesions of SS, the aim of current thesis was the investigation of the ability of SS patients’ macrophages for phagocytosis of apoptotic cells and of necrotic material. Compared to healthy blood donors (HBD), approximately half of SS and SLE patients studied (but not RA) were found to manifest significantly reduced phagocytosis of…

Subjects/Keywords: Σύνδρομο Sjogren's; Απόπτωση; Φαγοκυττάρωση; Λέμφωμα; Εξωκυττάριο DNA; Κυτταροπλασματικό DNA; Επιθηλιακά κύτταρα σιελογόνων αδένων; Υποδοχέας που ενεργοποιείται από παράγοντες που επάγουν τον πολλαπλασιασμό των υπεροξεισωμάτων τάξεως γ; Φλεγμονόσωμα; Sjogren's syndrome; Apoptosis; Phagocytosis; Lymphoma; Extracellular DNA; Cytosolic DNA; Salivary gland epithelial cells; Peroxisome proliferator - activated receptor gamma; Inflammasome

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

vakrakou, a. (2019). Μελέτη των μηχανισμών διαταραχής κάθαρσης αποπτωτικών και νευρωτικών κυττάρων στο Σύνδρομο Sjogren. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/45772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

vakrakou, aglaia. “Μελέτη των μηχανισμών διαταραχής κάθαρσης αποπτωτικών και νευρωτικών κυττάρων στο Σύνδρομο Sjogren.” 2019. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 03, 2021. http://hdl.handle.net/10442/hedi/45772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

vakrakou, aglaia. “Μελέτη των μηχανισμών διαταραχής κάθαρσης αποπτωτικών και νευρωτικών κυττάρων στο Σύνδρομο Sjogren.” 2019. Web. 03 Mar 2021.

Vancouver:

vakrakou a. Μελέτη των μηχανισμών διαταραχής κάθαρσης αποπτωτικών και νευρωτικών κυττάρων στο Σύνδρομο Sjogren. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10442/hedi/45772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

vakrakou a. Μελέτη των μηχανισμών διαταραχής κάθαρσης αποπτωτικών και νευρωτικών κυττάρων στο Σύνδρομο Sjogren. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. Available from: http://hdl.handle.net/10442/hedi/45772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brigham Young University

8. Brammer, Jeffrey M. Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana.

Degree: MS, 2010, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=3533&context=etd

► Plants have two organelles outside the nucleus which carry their own DNA, mitochondria and chloroplasts. These organelles are descendants of bacteria that were engulfed… (more)

▼ Plants have two organelles outside the nucleus which carry their own DNA, mitochondria and chloroplasts. These organelles are descendants of bacteria that were engulfed by their host according to the endosymbiotic theory. Over time, DNA has been exchanged between these organelles and the nucleus. Two polymerases, DNA Polymerases Gamma I and II, are encoded in the nucleus and remain under nuclear control, but are transported into the mitochondria and chloroplasts. DNA polymerases gamma I and II are two organelle polymerases which have been studied through sequence analysis and shown to localize to both mitochondria and chloroplasts. Little has been done to characterize the activities of these polymerases. Work in tobacco showed the homology of these polymerases to each other and to DNA Polymerase I in bacteria. They have been characterized as being part of the DNA Polymerase A family of polymerases. In my research I have studied the effect of T-DNA insertions within the DNA Polymerase Gamma I and II genes. Since these DNA Polymerases are targeted to the mitochondria and chloroplasts, I studied the effect of knocking out these genes. A plant heterozygous for an insert in DNA Polymerase Gamma I grows slightly slower than wild type plants with an approximately 20% reduction in mitochondrial and chloroplast DNA copy number. A plant homozygous for an insert in this same gene has a drastic phenotype with stunted plants that grow to around 1 inch tall, with floral stems, and have an approximately 50-55% reduction in mitochondrial and chloroplast DNA copy number. Wild type plants can grow to a height of 12-18 inches with floral stems as a comparison. A plant heterozygous for an insert in the DNA Polymerase Gamma II gene grows slightly slower than wild type plants and has an approximately 15% reduction in mitochondrial DNA copy number and a 50% reduction in chloroplast DNA copy number. These plants also produce much less seed than do other mutants and wild type plants.

Subjects/Keywords: DNA polymerase; Arabidopsis; Arabidopsis thaliana; gamma polymerase; mitochondrion; mitochondria; chloroplast; plastid; DNA replication; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brammer, J. M. (2010). Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=3533&context=etd

Chicago Manual of Style (16^th Edition):

Brammer, Jeffrey M. “Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana.” 2010. Masters Thesis, Brigham Young University. Accessed March 03, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=3533&context=etd.

MLA Handbook (7^th Edition):

Brammer, Jeffrey M. “Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana.” 2010. Web. 03 Mar 2021.

Vancouver:

Brammer JM. Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana. [Internet] [Masters thesis]. Brigham Young University; 2010. [cited 2021 Mar 03]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=3533&context=etd.

Council of Science Editors:

Brammer JM. Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana. [Masters Thesis]. Brigham Young University; 2010. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=3533&context=etd

University of Southern California

9. Barath, Manasi. Increased anti-cancer efficacy of a novel lipid modified analog of temozolomide in breast cancer cells.

Degree: MS, Molecular Microbiology and Immunology, 2013, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/243872/rec/3449

► Temozolomide (TMZ; an alkylating agent that is able to cross the blood brain barrier) is used for the treatment of malignant gliomas, but much less… (more)

▼ Temozolomide (TMZ; an alkylating agent that is able to cross the blood brain barrier) is used for the treatment of malignant gliomas, but much less is known about its effects in breast cancer. Brain tumors are more a result of other organ tumors metastasizing to the brain rather than tumors originating in the brain itself. 15% of brain neoplasms are a result of breast cancer metastases. Breast cancer that has metastasized to the brain becomes particularly difficult to treat as the regular breast cancer drugs do not cross the blood brain barrier. TMZ, with its different mechanism of action and ability to cross the blood brain barrier has long been viewed as a potentially useful chemotherapeutic agent for treating cancers other than brain malignancies. Its untapped potential as a chemotherapeutic agent for other cancers led to the development of its novel conjugate with Perillyl alcohol (POH) via a carbamate bond. POH is used to treat malignant gliomas via intranasal delivery. The interest for using the conjugate on breast cancers was because of two main factors, the first being that metastasis to the brain was a major problem of patients with breast cancer and the second being that there is targeted therapy for hormone receptor and her-2 over-expressing breast cancer whereas there is no targeted therapy as such for triple negative breast cancers (TNBC; negative for estrogen and progesterone receptors, and lacking overexpressed Her2/neu). Triple-negative breast cancer represents a particularly difficult to treat subtype of breast cancer that results in more aggressive tumors and bad prognosis. We compared TMZ side by side to TMZ-POH or T-P. Colony formation assays and Western blots were used to determine the DNA damaging and cytotoxic potency of the two drugs in a number of breast carcinoma cell lines. Our results show that both drugs are effective in causing DNA damage and cell death, but that T-P is significantly more potent than TMZ. Intriguingly, overexpression of the DNA damage repair protein MGMT confers resistance against TMZ as well as against T-P. Introduction of the MGMT gene into sensitive cells makes them resistant to both drugs; conversely inhibition of MGMT renders resistant cells sensitive. Neither does the Fatty Acid Transporter Protein (FATP) have a role to play in the uptake of TMZ-POH nor does the p-glycoprotein play a role in making cells less sensitive to the drug. Taken together, our results indicate that TMZ, and even more so its novel analog T-P, might hold promise for the treatment of intracranial breast cancer metastases, but that MGMT status of the tumor would have to be verified before the onset of therapy. Advisors/Committee Members: Schönthal, Axel (Committee Chair), Hofman, Florence M. (Committee Member), Ou, J.-H. James (Committee Member).

Subjects/Keywords: temozolomide; DNA damage; breast cancer; gamma H2AX; MGMT; TMZ-POH; brain metastases; DNA alkylating agents

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barath, M. (2013). Increased anti-cancer efficacy of a novel lipid modified analog of temozolomide in breast cancer cells. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/243872/rec/3449

Chicago Manual of Style (16^th Edition):

Barath, Manasi. “Increased anti-cancer efficacy of a novel lipid modified analog of temozolomide in breast cancer cells.” 2013. Masters Thesis, University of Southern California. Accessed March 03, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/243872/rec/3449.

MLA Handbook (7^th Edition):

Barath, Manasi. “Increased anti-cancer efficacy of a novel lipid modified analog of temozolomide in breast cancer cells.” 2013. Web. 03 Mar 2021.

Vancouver:

Barath M. Increased anti-cancer efficacy of a novel lipid modified analog of temozolomide in breast cancer cells. [Internet] [Masters thesis]. University of Southern California; 2013. [cited 2021 Mar 03]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/243872/rec/3449.

Council of Science Editors:

Barath M. Increased anti-cancer efficacy of a novel lipid modified analog of temozolomide in breast cancer cells. [Masters Thesis]. University of Southern California; 2013. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/243872/rec/3449

Vanderbilt University

10. Williams, Christopher Lawrence. Instability of an epigenetic mark: T-bet and STAT4 influence the symmetry and plasticity of DNA methylation at the IFN-γ promoter in effector and memory Th2 lymphocytes.

Degree: PhD, Microbiology and Immunology, 2013, Vanderbilt University

URL: http://hdl.handle.net/1803/13450

► CD4+ T cells developing toward a Th2 fate express IL-4, IL-5, and IL-13 while inhibiting production of cytokines associated with other Th types, such as… (more)

Subjects/Keywords: CD4 T cell memory; DNA methylation; T-bet; STAT4; Interferon gamma

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Williams, C. L. (2013). Instability of an epigenetic mark: T-bet and STAT4 influence the symmetry and plasticity of DNA methylation at the IFN-γ promoter in effector and memory Th2 lymphocytes. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13450

Chicago Manual of Style (16^th Edition):

Williams, Christopher Lawrence. “Instability of an epigenetic mark: T-bet and STAT4 influence the symmetry and plasticity of DNA methylation at the IFN-γ promoter in effector and memory Th2 lymphocytes.” 2013. Doctoral Dissertation, Vanderbilt University. Accessed March 03, 2021. http://hdl.handle.net/1803/13450.

MLA Handbook (7^th Edition):

Williams, Christopher Lawrence. “Instability of an epigenetic mark: T-bet and STAT4 influence the symmetry and plasticity of DNA methylation at the IFN-γ promoter in effector and memory Th2 lymphocytes.” 2013. Web. 03 Mar 2021.

Vancouver:

Williams CL. Instability of an epigenetic mark: T-bet and STAT4 influence the symmetry and plasticity of DNA methylation at the IFN-γ promoter in effector and memory Th2 lymphocytes. [Internet] [Doctoral dissertation]. Vanderbilt University; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1803/13450.

Council of Science Editors:

Williams CL. Instability of an epigenetic mark: T-bet and STAT4 influence the symmetry and plasticity of DNA methylation at the IFN-γ promoter in effector and memory Th2 lymphocytes. [Doctoral Dissertation]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/13450

Brigham Young University

11. Cupp, John D. Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated.

Degree: PhD, 2012, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4746&context=etd

► Plant mitochondrial genomes are large and complex, and the mechanisms for maintaining mitochondrial DNA (mtDNA) remain unclear. Arabidopsis thaliana has two DNA polymerase genes, polIA… (more)

Subjects/Keywords: polymerase gamma; PolIA; PolIB; TWINKLE; mitochondria; DNA replication; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cupp, J. D. (2012). Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated. (Doctoral Dissertation). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4746&context=etd

Chicago Manual of Style (16^th Edition):

Cupp, John D. “Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated.” 2012. Doctoral Dissertation, Brigham Young University. Accessed March 03, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4746&context=etd.

MLA Handbook (7^th Edition):

Cupp, John D. “Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated.” 2012. Web. 03 Mar 2021.

Vancouver:

Cupp JD. Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated. [Internet] [Doctoral dissertation]. Brigham Young University; 2012. [cited 2021 Mar 03]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4746&context=etd.

Council of Science Editors:

Cupp JD. Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated. [Doctoral Dissertation]. Brigham Young University; 2012. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4746&context=etd

Université Catholique de Louvain

12. Szczepanowska, Karolina. A cluster of pathogenic mutations in the 3'-5' exonuclease domain of DNA polymerase gamma defines a novel module coupling DNA synthesis and degradation.

Degree: 2011, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/73438

►

DNA polymerase gamma (pol g in human, Mip1 in yeast) is the unique DNA replicase found in mitochondria. Pol g plays a key role in… (more)

Subjects/Keywords: Mitochondrial DNA polymerase gamma; Mitochondrial disorders; Mutations; Yeast

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Szczepanowska, K. (2011). A cluster of pathogenic mutations in the 3'-5' exonuclease domain of DNA polymerase gamma defines a novel module coupling DNA synthesis and degradation. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/73438

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Szczepanowska, Karolina. “A cluster of pathogenic mutations in the 3'-5' exonuclease domain of DNA polymerase gamma defines a novel module coupling DNA synthesis and degradation.” 2011. Thesis, Université Catholique de Louvain. Accessed March 03, 2021. http://hdl.handle.net/2078.1/73438.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Szczepanowska, Karolina. “A cluster of pathogenic mutations in the 3'-5' exonuclease domain of DNA polymerase gamma defines a novel module coupling DNA synthesis and degradation.” 2011. Web. 03 Mar 2021.

Vancouver:

Szczepanowska K. A cluster of pathogenic mutations in the 3'-5' exonuclease domain of DNA polymerase gamma defines a novel module coupling DNA synthesis and degradation. [Internet] [Thesis]. Université Catholique de Louvain; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2078.1/73438.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Szczepanowska K. A cluster of pathogenic mutations in the 3'-5' exonuclease domain of DNA polymerase gamma defines a novel module coupling DNA synthesis and degradation. [Thesis]. Université Catholique de Louvain; 2011. Available from: http://hdl.handle.net/2078.1/73438

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

13. Ziehr, Jessica Lea. Kinetics and specificity of human mitochondrial DNA polymerase gamma and HIV-1 reverse transcriptase.

Degree: PhD, Cell and Molecular Biology, 2014, University of Texas – Austin

URL: http://hdl.handle.net/2152/31296

► The human mitochondrial DNA (mtDNA) genome must be faithfully maintained by the mitochondrial DNA replication machinery. Deficiencies in mtDNA maintenance result in the accumulation of… (more)

▼ The human mitochondrial DNA (mtDNA) genome must be faithfully maintained by the mitochondrial DNA replication machinery. Deficiencies in mtDNA maintenance result in the accumulation of mutations and deletions, which have been associated with a number of neuromuscular degenerative disorders including, mtDNA depletion syndrome, Alpers syndrome, progressive external opthalmoplegia (PEO), and sensory ataxic neuropathy, dysarthria, and opthalmoparesis (SANDO). The mtDNA replication machinery is comprised of a nuclearly-encoded DNA polymerase gamma (Pol γ), single-stranded DNA binding protein (mtSSB), and a hexameric mtDNA helicase. In this work, we employed quantitative pre-steady state kinetic techniques to establish the mechanisms responsible for the replication of the human mitochondrial DNA by Pol γ and explored the effects of point mutations that are observed in heritable diseases. With our biochemical characterization of mutants of Pol γ, we have shown unique characteristics that would lead to profound physiological consequences over time. Additionally, we have made significant progress towards reconstitution of the mitochondrial DNA replisome by monitoring DNA polymerization that is dependent on helicase unwinding of double stranded DNA. Overall, this work provides a better understanding of the mechanism of mtDNA replication and has important implications toward understanding the role of mitochondrial DNA replication in mitochondrial disease, ageing and cancer. In addition to the work on the mtDNA replisome, we have applied pre-steady state kinetic techniques to better understand the mechanism of RNA-dependent DNA polymerization by HIV reverse transcriptase (HIV-RT). This enzyme is responsible for the replication of the viral genome in HIV and is a common target for anti-HIV drugs. We have characterized the role of enzyme conformational changes in the kinetics of incorporation of correct nucleotide and the Nucleotide Reverse Transcriptase Inhibitor (NRTI) AZT by wild-type enzyme, as well as a mutant with clinical resistance to AZT. This work provides a better understanding of the complete mechanism of RNA-dependent DNA polymerization, the changes in the mechanism in the presence of inhibitor and the development of resistance to this nucleoside analog; and thereby this work contributes to the long-term goal of designing more effective drugs that can possibly deter resistance and be used successfully for treatment of HIV. Advisors/Committee Members: Johnson, Kenneth A. (Kenneth Allen) (advisor), Lambowitz, Alan M (committee member), Russell, Rick (committee member), Marcotte, Edward M (committee member), Dalby, Kevin (committee member).

Subjects/Keywords: DNA polymerase; Pre-steady state kinetics; HIV reverse transcriptase; Polymerase gamma

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ziehr, J. L. (2014). Kinetics and specificity of human mitochondrial DNA polymerase gamma and HIV-1 reverse transcriptase. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/31296

Chicago Manual of Style (16^th Edition):

Ziehr, Jessica Lea. “Kinetics and specificity of human mitochondrial DNA polymerase gamma and HIV-1 reverse transcriptase.” 2014. Doctoral Dissertation, University of Texas – Austin. Accessed March 03, 2021. http://hdl.handle.net/2152/31296.

MLA Handbook (7^th Edition):

Ziehr, Jessica Lea. “Kinetics and specificity of human mitochondrial DNA polymerase gamma and HIV-1 reverse transcriptase.” 2014. Web. 03 Mar 2021.

Vancouver:

Ziehr JL. Kinetics and specificity of human mitochondrial DNA polymerase gamma and HIV-1 reverse transcriptase. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152/31296.

Council of Science Editors:

Ziehr JL. Kinetics and specificity of human mitochondrial DNA polymerase gamma and HIV-1 reverse transcriptase. [Doctoral Dissertation]. University of Texas – Austin; 2014. Available from: http://hdl.handle.net/2152/31296

14. Ahmed, Omar Jamil. Interpreting the Rhythms of the Hippocampus and Neocortex.

Degree: PhD, Neuroscience, 2010, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:11044/

► When groups of neurons are synchronously and rhythmically active, they give rise to electrical oscillations that can be seen in the surrounding extracellular local field… (more)

▼ When groups of neurons are synchronously and rhythmically active, they give rise to electrical oscillations that can be seen in the surrounding extracellular local field potential (LFP) and in scalp electroencephalography (EEG) recordings. The frequencies of these oscillations range from slow (0.3-4 Hz) delta oscillations during sleep to very fast (300 Hz) ripple oscillations during epileptic seizures. The appearance of intermediate gamma frequency oscillations (40-80 Hz) is associated with active sensory processing, increased attention and improved reaction times. Gamma rhythms are often altered in patients diagnosed with schizophrenia, bipolar disorder, depression, ADHD, tinnitus and epilepsy, suggesting that a deeper understanding of the gamma signal may help to elucidate the differences between normal and pathological neural circuits.Using tetrode recordings from the hippocampus and neocortex of freely behaving rats, this thesis examines both the behavioral and mechanistic underpinnings of gamma oscillations. We show that increased running speed is accompanied by large, systematic increases in the frequency of hippocampal CA1 network oscillations spanning the entire gamma range and beyond. These speed-dependent changes are seen on both linear tracks and two-dimensional platforms, and are thus independent of spatial environment. We hypothesize that the faster gamma oscillations may be indicative of faster reaction times and improved perception at faster running speeds.Individual gamma cycles are less than 20 ms in duration and show large, cycle-by-cycle changes in amplitude and frequency. We also show that these rapid LFP changes are accompanied by instantaneous, cycle-by-cycle changes in spike-timing precision of cells in the neocortex. The slope of an individual LFP gamma cycle encodes the amount of spike synchrony during that gamma cycle, but provides no information about synchrony in the very next cycle. These changes in spike synchrony are preceded by corresponding changes in spike rate in the previous gamma cycle. Thus, local cortical circuits transform asynchronous increases in spike rate into fewer, but more synchronous spikes within a single gamma cycle. This sequence can be reliably predicted by the slope of individual gamma cycles, with important implications for the design of brain-machine interfaces. Advisors/Committee Members: Connors, Barry (Director), Mehta, Mayank (Director), Lipscombe, Diane (Reader), Burwell, Rebecca (Reader), Knierim, James (Reader).

Subjects/Keywords: gamma

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahmed, O. J. (2010). Interpreting the Rhythms of the Hippocampus and Neocortex. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:11044/

Chicago Manual of Style (16^th Edition):

Ahmed, Omar Jamil. “Interpreting the Rhythms of the Hippocampus and Neocortex.” 2010. Doctoral Dissertation, Brown University. Accessed March 03, 2021. https://repository.library.brown.edu/studio/item/bdr:11044/.

MLA Handbook (7^th Edition):

Ahmed, Omar Jamil. “Interpreting the Rhythms of the Hippocampus and Neocortex.” 2010. Web. 03 Mar 2021.

Vancouver:

Ahmed OJ. Interpreting the Rhythms of the Hippocampus and Neocortex. [Internet] [Doctoral dissertation]. Brown University; 2010. [cited 2021 Mar 03]. Available from: https://repository.library.brown.edu/studio/item/bdr:11044/.

Council of Science Editors:

Ahmed OJ. Interpreting the Rhythms of the Hippocampus and Neocortex. [Doctoral Dissertation]. Brown University; 2010. Available from: https://repository.library.brown.edu/studio/item/bdr:11044/

15. Khonsari, Hassan. Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts.

Degree: PhD, 2015, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/12889 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687647

► Friedreich ataxia (FRDA) is a progressive neurodegenerative disease with primary sites of pathology in the large sensory neurons of the dorsal root ganglia (DRG) and… (more)

▼ Friedreich ataxia (FRDA) is a progressive neurodegenerative disease with primary sites of pathology in the large sensory neurons of the dorsal root ganglia (DRG) and dentate nucleus of the cerebellum. FRDA is also often accompanied by severe cardiomyopathy and diabetes mellitus. FRDA is caused by loss of frataxin (FXN) expression, which is due to GAA repeat expansion in intron 1 of the FXN gene. Frataxin is a mitochondrial protein important in iron-sulphur cluster (ISC) biogenesis and in the electron transport chain (ETC). As a consequence of impaired mitochondrial energy metabolism, FRDA cells show increased levels of and sensitivity to oxidative stress, which is known to be associated with genome instability. In this study, we investigated DNA damage/repair in relation to FXN expression via immunostaining of γ-H2AX, a nuclear protein that is recruited to DNA double strand breaks (DSBs). We found FRDA patient and YG8sR FRDA mouse model fibroblasts to have inherently elevated DNA DSBs (1.8 and 0.9 foci/nucleus) compared to normal fibroblasts (0.6 and 0.2 foci/nucleus, in each case P < 0.001). By delivering the FXN gene to these cells with a lentivirus vector (LV) at a copy number of ~1/cell, FXN mRNA levels reached 48 fold (patient cells) and 42 fold (YG8sR cells) and protein levels reached 20 fold (patient cells) and 3.5 fold (YG8sR cells) that of untreated fibroblasts, without observable cytotoxicity. This resulted in a reduction in DNA DSB foci to 0.7 and 0.43 (in each case P < 0.001) in human and YG8sR fibroblasts, respectively and an increase in cell survival to that found for normal fibroblasts. We next irradiated the FRDA fibroblasts (2Gy) and measured their DSB repair profiles. Both human and mouse FRDA fibroblasts were unable to repair damaged DNA. However, repair returned to near normal levels following LV FXN gene transfer. Our data suggest frataxin may be important for genome stability and cell survival by ensuring ISC for DNA damage repair enzymes or may be required directly for DNA DSB repair.

Subjects/Keywords: 616.8; Gene therapy; DNA double strand break; Gamma-HZAX; Mitochondria; FRDA correction

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khonsari, H. (2015). Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/12889 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687647

Chicago Manual of Style (16^th Edition):

Khonsari, Hassan. “Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts.” 2015. Doctoral Dissertation, Brunel University. Accessed March 03, 2021. http://bura.brunel.ac.uk/handle/2438/12889 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687647.

MLA Handbook (7^th Edition):

Khonsari, Hassan. “Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts.” 2015. Web. 03 Mar 2021.

Vancouver:

Khonsari H. Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts. [Internet] [Doctoral dissertation]. Brunel University; 2015. [cited 2021 Mar 03]. Available from: http://bura.brunel.ac.uk/handle/2438/12889 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687647.

Council of Science Editors:

Khonsari H. Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts. [Doctoral Dissertation]. Brunel University; 2015. Available from: http://bura.brunel.ac.uk/handle/2438/12889 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687647

16. Saad, Lina. Caractérisation moléculaire de la forme résistante de la leucémie lymphocytaire chronique (LLC) : rôle fonctionnel de la nouvelle forme phosphorylée de Ku70 : Molecular characterization of resistant chronic lymphocytic leukemia (CLL) : function of a new phosphorylated form of Ku70.

Degree: Docteur es, Biologie moléculaire et cellulaire, 2013, Université Paris Descartes – Paris V

URL: http://www.theses.fr/2013PA05P613

►

Nous avons identifié une nouvelle forme de phospho-S27-S33-Ku70 constitutivement surexprimée dans des cellules issues de la leucémie lymphocytaire chronique résistante à la chimiothérapie basée sur… (more)

▼

Nous avons identifié une nouvelle forme de phospho-S27-S33-Ku70 constitutivement surexprimée dans des cellules issues de la leucémie lymphocytaire chronique résistante à la chimiothérapie basée sur des agents alkylants de l’ADN et/ou analogues nucléotidiques. La protéine Ku70 est une protéine essentielle du maintien de la stabilité génomique par son rôle dans la réparation non-homologue (système NHEJ) des cassures double brin de l’ADN (CDB) et par sa fonction télomérique. Le laboratoire d’accueil a déjà démontré, in vitro et in vivo, dans les cellules LLC résistantes une altération de la réparation par le système NHEJ et un dysfonctionnement télomérique. Le travail de thèse a porté sur la caractérisation fonctionnelle de cette nouvelle forme phospho-S27-S33-Ku70. Pour ceci, nous avons utilisé des vecteurs d’expression permettant simultanément d’inhiber l’expression du Ku70 endogène (shRNA) et d’exprimer de façon épisomale différentes formes de Ku70 exogène. Ainsi, nous avons démontré : i) une stricte colocalisation de pS27-pS33-Ku70 avec les foyers γ-H2AX; ii) des cassures double brin (DSB) induisent la phosphorylation de S27-S33-Ku70 sous forme hétérodimère avec Ku80. Cette phosphorylation a lieu quelques minutes après le stress génotoxique et implique l'activité et l'interaction physique avec pS2056-DNA-PKcs, reliant ainsi pS27-pS33-Ku70 au système NHEJ ; iii) les cellules exprimant la forme sauvage exogène S27-S33-Ku70 ou la forme phosphomimétique E27-E33-Ku70 présentent une cinétique de réparation de l’ADN plus rapide que celle des cellules exprimant la forme mutée A27-A33-Ku70. Cependant, iv) la forme sauvage de Ku70 contribue à un niveau plus élevé d'aberrations structurales chromosomiques après la première division cellulaire suite à un stress génotoxique indiquant une infidélité lors de la réparation des dommages de l’ADN. En outre, les cellules exprimant A27-A33-Ku70 possèdent un index cellulaire plus élevé qui est corrélé avec une activation de la voie β-caténine. En adéquation avec sa surexpression dans la forme résistante de la LLC, l’ensemble de ces résultats suggère un rôle oncogénique de la forme phosphorylée de Ku70. Nous avons ensuite testé l’effet des nanodiamants hydrogénés (ND-H) dans des lignées exprimant différentes formes de Ku70. Grâce à leurs propriétés physico-chimiques les ND-H sont capables de potentialiser sous irradiation la production intracellulaire des espèces réactives de l’oxygène (ROS) et ainsi augmenter le taux des cassures (simple et double brin de l’ADN) et solliciter d’avantage le système de réparation de l’ADN. Nous observons que indépendamment de la forme exprimée de Ku70, ce double traitement induisait la sénescence cellulaire ; une découverte d’un intérêt à la fois fondamental (compréhension des voies apoptotiques vs senescence) et d’utilité pharmacologique potentielle.

We have identified a new form of phospho-S27-S33-Ku70 constitutively overexpressed in a subset of chronic lymphocytic leukemia (CLL) B cells resistant to apoptosis induced by DNA double strand breaks…

Advisors/Committee Members: Delic, Jozo (thesis director).

Subjects/Keywords: LLC; Résistance au stress génotoxique; Phospho-Ku70; Réparation ADN/instabilité génomique; ShRNA; Γ-H2AX; Test de Comètes; CLL; Resistance to genotoxic stress; Phospho-Ku70; DNA repair/genome stability; ShRNA; Γ-H2AX; Comet assay; 616.994 19

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saad, L. (2013). Caractérisation moléculaire de la forme résistante de la leucémie lymphocytaire chronique (LLC) : rôle fonctionnel de la nouvelle forme phosphorylée de Ku70 : Molecular characterization of resistant chronic lymphocytic leukemia (CLL) : function of a new phosphorylated form of Ku70. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2013PA05P613

Chicago Manual of Style (16^th Edition):

Saad, Lina. “Caractérisation moléculaire de la forme résistante de la leucémie lymphocytaire chronique (LLC) : rôle fonctionnel de la nouvelle forme phosphorylée de Ku70 : Molecular characterization of resistant chronic lymphocytic leukemia (CLL) : function of a new phosphorylated form of Ku70.” 2013. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed March 03, 2021. http://www.theses.fr/2013PA05P613.

MLA Handbook (7^th Edition):

Saad, Lina. “Caractérisation moléculaire de la forme résistante de la leucémie lymphocytaire chronique (LLC) : rôle fonctionnel de la nouvelle forme phosphorylée de Ku70 : Molecular characterization of resistant chronic lymphocytic leukemia (CLL) : function of a new phosphorylated form of Ku70.” 2013. Web. 03 Mar 2021.

Vancouver:

Saad L. Caractérisation moléculaire de la forme résistante de la leucémie lymphocytaire chronique (LLC) : rôle fonctionnel de la nouvelle forme phosphorylée de Ku70 : Molecular characterization of resistant chronic lymphocytic leukemia (CLL) : function of a new phosphorylated form of Ku70. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2013. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2013PA05P613.

Council of Science Editors:

Saad L. Caractérisation moléculaire de la forme résistante de la leucémie lymphocytaire chronique (LLC) : rôle fonctionnel de la nouvelle forme phosphorylée de Ku70 : Molecular characterization of resistant chronic lymphocytic leukemia (CLL) : function of a new phosphorylated form of Ku70. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2013. Available from: http://www.theses.fr/2013PA05P613

17. Giraud, Rémi. Influence de l'histoire thermique sur les propriétés mécaniques à haute et très haute température du superalliage monocristallin CMSX-4® :.

Degree: Docteur es, Mécanique des solides, des matériaux, des structures et des surfaces, 2014, Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique

URL: http://www.theses.fr/2014ESMA0005

►

Ce mémoire de thèse est consacré à une étude du fluage isotherme et anisotherme à diversniveaux de température et à l’analyse fine des évolutions microstructurales… (more)

▼

Ce mémoire de thèse est consacré à une étude du fluage isotherme et anisotherme à diversniveaux de température et à l’analyse fine des évolutions microstructurales associées à diverschargements thermomécaniques imposés du superalliage monocristallin, CMSX-4®. Cetravail est complété par la validation d’un modèle de comportement mécanique permettantd’intégrer ces évolutions microstructurales (fraction volumique des phases en présence,largeur des couloirs de matrice gamma, ...) pour déterminer la durée de vie du matériau.Parallèlement, le rôle de la microstructure initiale du matériau associée aux traitementsthermiques imposés dans la phase d’élaboration a été étudié. Il a été montré un effet marquéde cette microstructure initiale lors des essais de fluage à basse température (< 900°C), ainsique lors des essais de fluage anisotherme. Une microstructure de précipitation en radeaux detype N a été montrée comme particulièrement néfaste pour la résistance en fluageanisotherme. Enfin, le comportement du CMSX-4® est systématiquement comparé à celuiprécédemment établi dans des conditions similaires sur d’autres superalliages monocristallinspour pales haute pression.

This thesis is dedicated to the study of the isothermal and non-isothermal creep behavior in awide temperature range, and to the analysis of the microstructural evolution during differentthermomechanical paths on the superalloy single crystal, CMSX-4®. Moreover, the validationof a mechanical behavior model validation completes this work. This model takes intoaccount the microstructural evolutions (e.g. phase volume fraction, gamma-matrix channelwidth…) to predict the creep life. Additionally, the effect of the initial microstructure fromthe heat treatment has been investigated. It has been shown a strong impact of the as-receivedmicrostructure on the low temperature isothermal creep properties, and during non-isothermalcreep. A N-type rafted microstructure has been shown to be particularly detrimental to thenon-isothermal creep properties. Finally, the behavior of the CMSX-4® has been comparedwith the previous studies realized in the same conditions on other single crystal superalloysfor blades applications.

Advisors/Committee Members: Méndez, José (thesis director), Cormier, Jonathan (thesis director), Milhet, Xavier (thesis director), Hervier, Zéline (thesis director).

Subjects/Keywords: Superalliage base nickel; Anisothermie; Microstructure γ/γ’

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giraud, R. (2014). Influence de l'histoire thermique sur les propriétés mécaniques à haute et très haute température du superalliage monocristallin CMSX-4® :. (Doctoral Dissertation). Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique. Retrieved from http://www.theses.fr/2014ESMA0005

Chicago Manual of Style (16^th Edition):

Giraud, Rémi. “Influence de l'histoire thermique sur les propriétés mécaniques à haute et très haute température du superalliage monocristallin CMSX-4® :.” 2014. Doctoral Dissertation, Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique. Accessed March 03, 2021. http://www.theses.fr/2014ESMA0005.

MLA Handbook (7^th Edition):

Giraud, Rémi. “Influence de l'histoire thermique sur les propriétés mécaniques à haute et très haute température du superalliage monocristallin CMSX-4® :.” 2014. Web. 03 Mar 2021.

Vancouver:

Giraud R. Influence de l'histoire thermique sur les propriétés mécaniques à haute et très haute température du superalliage monocristallin CMSX-4® :. [Internet] [Doctoral dissertation]. Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique; 2014. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2014ESMA0005.

Council of Science Editors:

Giraud R. Influence de l'histoire thermique sur les propriétés mécaniques à haute et très haute température du superalliage monocristallin CMSX-4® :. [Doctoral Dissertation]. Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique; 2014. Available from: http://www.theses.fr/2014ESMA0005

18. Delattre, Marie-Coralie. Étude de la structure à basse énergie de ⁷⁹Zn par décroissance β et β-n de ⁷⁹Cu et ⁸⁰Cu : Study of the low energy structure of ⁷⁹Zn through β and β-n decay of ⁷⁹Cu and ⁸⁰Cu.

Degree: Docteur es, Structure et réactions nucléaires, 2016, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2016SACLS369

►

Afin de sonder la persistance de la fermeture de couches à N=50 pour les isotopes de nickel, une expérience a été réalisée au RIBF (RIKEN,… (more)

▼

Afin de sonder la persistance de la fermeture de couches à N=50 pour les isotopes de nickel, une expérience a été réalisée au RIBF (RIKEN, Japon) pour produire des noyaux autour du ⁷⁸Ni par fission en vol d'un faisceau de ²³⁸U. Les spectromètres BigRIPS et ZeroDegree ont été utilisés pour la purification et la sélection des noyaux d'intérêt. La décroissance de ces noyaux a été observée avec le dispositif EURICA pour la détection des rayonnements gamma, couplé avec le détecteur à particules chargées WAS3aBi.L'analyse des corrélations entre l'implantation des ions ⁷⁹′⁸⁰Cu et leur décroissance, ainsi que les spectres de décroissance gamma en simple et double coïncidence, nous ont permis de proposer un schéma de niveaux excités pour le ⁷⁹Zn, avec assignation de spin et parité pour la plupart des états. Nous avons aussi mis une contrainte entre 3hbar et 5hbar sur la valeur du spin de l'état fondamental du ⁸⁰Cu. La comparaison avec un modèle schématique de couplage cœur-particule nous a permis d’interpréter le schéma de niveau et de proposer des hypothèses sur la nature des états observés. Les énergies des états de particule individuelle extraites du calcul théorique indiquent la persistance du gap N=50 à Z=28.

In order to investigate the persistence of the N=50 gap for the Nickel isotopes, an experiment has been performed at RIBF (RIKEN, Japan) to produce exotic nuclei around ⁷⁸Ni by in-flight fission of a ²³⁸U beam. The BigRIPS and ZeroDegree spectrometers were used to purify and select the nuclei of interest. Their decay was detected with the EURICA detector for γ-ray detection, coupled with the charged particle WAS3aBi detector. The analysis of the correlations between the ion implantation and their decay, as well as the γ decay spectra in simple and double coincidence allowed us to propose a level scheme of the excited states for the ⁷⁹Zn, and we assigned the spin and parity for most of the states. We also constrained the spin value for the fundamental state of the ⁸⁰Cu, between 3hbar and 5hbar. Comparison with a core-particle coupling model allowed us to interpret the level scheme and propose hypothesis about the nature of the observed states. The single particle state energies extracted from the theoretical calculation indicate the persistence of the N=50 gap at Z=28.

Advisors/Committee Members: Verney, David (thesis director).

Subjects/Keywords: Nombres magiques; Noyaux exotiques; ⁷⁸Ni; 78Ni; Spectroscopie γ; Spectroscopie gamma; Magic numbers; Exotic nuclei; ⁷⁸Ni; 78Ni; . γ spectroscopy; Gamma spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Delattre, M. (2016). Étude de la structure à basse énergie de ⁷⁹Zn par décroissance β et β-n de ⁷⁹Cu et ⁸⁰Cu : Study of the low energy structure of ⁷⁹Zn through β and β-n decay of ⁷⁹Cu and ⁸⁰Cu. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLS369

Chicago Manual of Style (16^th Edition):

Delattre, Marie-Coralie. “Étude de la structure à basse énergie de ⁷⁹Zn par décroissance β et β-n de ⁷⁹Cu et ⁸⁰Cu : Study of the low energy structure of ⁷⁹Zn through β and β-n decay of ⁷⁹Cu and ⁸⁰Cu.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 03, 2021. http://www.theses.fr/2016SACLS369.

MLA Handbook (7^th Edition):

Delattre, Marie-Coralie. “Étude de la structure à basse énergie de ⁷⁹Zn par décroissance β et β-n de ⁷⁹Cu et ⁸⁰Cu : Study of the low energy structure of ⁷⁹Zn through β and β-n decay of ⁷⁹Cu and ⁸⁰Cu.” 2016. Web. 03 Mar 2021.

Vancouver:

Delattre M. Étude de la structure à basse énergie de ⁷⁹Zn par décroissance β et β-n de ⁷⁹Cu et ⁸⁰Cu : Study of the low energy structure of ⁷⁹Zn through β and β-n decay of ⁷⁹Cu and ⁸⁰Cu. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2016SACLS369.

Council of Science Editors:

Delattre M. Étude de la structure à basse énergie de ⁷⁹Zn par décroissance β et β-n de ⁷⁹Cu et ⁸⁰Cu : Study of the low energy structure of ⁷⁹Zn through β and β-n decay of ⁷⁹Cu and ⁸⁰Cu. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLS369

19. Vaurijoux, Aurélie. Étude des conséquences génétiques et épigénétiques consécutives à la signalisation persistante des dommages radio-induits de l'ADN : Study of genetic and epigenetic consequences consecutive to the persistent signaling of radiation-induced DNA damage.

Degree: Docteur es, Sciences de la vie et de la santé, 2016, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2016SACLS515

► Les cassures double-brin de l’ADN (CDB) sont des événements clés dans la réponse aux rayonnements ionisants qui, avec le profil génétique et épigénétique individuel, peuvent… (more)

▼ Les cassures double-brin de l’ADN (CDB) sont des événements clés dans la réponse aux rayonnements ionisants qui, avec le profil génétique et épigénétique individuel, peuvent conditionner le devenir des tissus sains d’un individu exposé. À la suite des cassures de la molécule d’ADN et de la déstabilisation de la chromatine, une série de modifications post-traductionnelles des histones se produit, notamment la phosphorylation de la serine 139 de l'histone H2A.X (gamma-H2A.X), conduisant à la formation de foyers radio-induits. La réparation des CDB, et donc la disparition de ces foyers, a lieu dans les heures suivant l’exposition. Toutefois, une certaine proportion de ces foyers gamma-H2A.X persiste 24 heures après l’irradiation. La nature et le rôle de ces foyers persistants sont encore peu clairs. L’objectif de ce travail est d'explorer les caractéristiques de ces foyers persistants et leurs conséquences sur le devenir des cellules. Pour étudier la dynamique des foyers radio-induits, nous avons exposé des HUVEC synchronisées en phase G0/G1 à des doses de 1 et 5 Gy de rayons X. Les foyers radio-induits ont été étudiés à partir de 10 minutes et jusqu'à 7 jours après l'exposition par l’analyse de gamma-H2A.X et de l’association temporelle de la protéine 53BP1 et des CN-PML (corps nucléaires PML). L’impact des foyers persistants sur la prolifération cellulaire a également été exploré. Nous avons analysé en microscopie à fluorescence une moyenne de 4 000 cellules pour chaque condition à l'aide d'une analyse d’image permettant la détection automatique des noyaux et des foyers. L'analyse d'un grand nombre d‘évènements nous a permis de discriminer des sous-populations de cellules ou de foyers sur la base de différentes caractéristiques, telles que leur aire ou la phase du cycle cellulaire, et de mesurer leur représentativité dans l'ensemble de la population de cellules exposées. Ainsi, nous avons déterminé que les foyers gamma-H2A.X persistant ont une aire supérieure à 0,72 ± 0,11 µm² et qu’ils sont toujours colocalisés avec 53BP1. Plus de 70% des cellules exposées à 5 Gy ont au moins un foyer persistant 24 heures après l'exposition. De plus, ces foyers persistants sont observables au moins jusqu'à 7 jours après l’irradiation. Une association spatiale significative entre les CN-PML et les foyers gamma-H2A.X a été observée à partir de 10 minutes après l'exposition et 24 heures après l’exposition, environ 90% des foyers persistants sont associés à un CN-PML. De plus, la présence de foyers persistants ne bloque pas définitivement la prolifération des cellules. Cependant, la fréquence des foyers persistants est plus faible dans les cellules filles que dans les cellules irradiées, probablement en raison d'une certaine proportion de distribution asymétrique des foyers persistants entre les cellules filles. Nous avons également mesuré une corrélation positive entre la présence d'un foyer persistant et la probabilité de mauvaise ségrégation de l'ADN par l'observation de phénomènes de catastrophes mitotiques. Il semble donc que la… Advisors/Committee Members: Barquinero, Joan Francesc (thesis director).

Subjects/Keywords: Rayonnements ionisants; Foyers gamma-H2A.X; Cassures double-Brins; Corps nucléaires PML; Division cellulaire; Ionizing radiation; Gamma-H2A.X foci; DNA double strand break; Promyelocytic leukemia nuclear bodies; Cell division

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaurijoux, A. (2016). Étude des conséquences génétiques et épigénétiques consécutives à la signalisation persistante des dommages radio-induits de l'ADN : Study of genetic and epigenetic consequences consecutive to the persistent signaling of radiation-induced DNA damage. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLS515

Chicago Manual of Style (16^th Edition):

Vaurijoux, Aurélie. “Étude des conséquences génétiques et épigénétiques consécutives à la signalisation persistante des dommages radio-induits de l'ADN : Study of genetic and epigenetic consequences consecutive to the persistent signaling of radiation-induced DNA damage.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed March 03, 2021. http://www.theses.fr/2016SACLS515.

MLA Handbook (7^th Edition):

Vaurijoux, Aurélie. “Étude des conséquences génétiques et épigénétiques consécutives à la signalisation persistante des dommages radio-induits de l'ADN : Study of genetic and epigenetic consequences consecutive to the persistent signaling of radiation-induced DNA damage.” 2016. Web. 03 Mar 2021.

Vancouver:

Vaurijoux A. Étude des conséquences génétiques et épigénétiques consécutives à la signalisation persistante des dommages radio-induits de l'ADN : Study of genetic and epigenetic consequences consecutive to the persistent signaling of radiation-induced DNA damage. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2016SACLS515.

Council of Science Editors:

Vaurijoux A. Étude des conséquences génétiques et épigénétiques consécutives à la signalisation persistante des dommages radio-induits de l'ADN : Study of genetic and epigenetic consequences consecutive to the persistent signaling of radiation-induced DNA damage. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLS515

20. Trijau, Marie. Approche moléculaire et mécaniste de la réponse transgénérationnelle lors d'une irradiation gamma chronique chez le cladocère Daphnia magna : Molecular and mechanistic studies of the transgenerational response during a chronic gamma irradiation in the cladoceran Daphnia magna.

Degree: Docteur es, Océanographie, 2018, Aix Marseille Université

URL: http://www.theses.fr/2018AIXM0467

►

Afin de protéger durablement les écosystèmes face aux rejets planifiés ou accidentels de radionucléides dans l’environnement, il est essentiel d’évaluer l’impact de l’exposition des organismes… (more)

▼

Afin de protéger durablement les écosystèmes face aux rejets planifiés ou accidentels de radionucléides dans l’environnement, il est essentiel d’évaluer l’impact de l’exposition des organismes aux radiations ionisantes sur le long terme, à l’échelle de plusieurs générations. Dans ce contexte, ce travail de doctorat vise à améliorer la caractérisation des processus moléculaires et la prédiction des effets transgénérationnels lors d’une exposition aux radiations gamma. Une approche expérimentale concerne l’étude des modifications épigénétiques radio-induites, c’est-à-dire des modifications des mécanismes régulant l’activité des gènes sans modification de la séquence d’ADN elle-même et de leur transmission au fil des générations. Cette étude a permis de mettre en évidence que certaines modifications de la méthylation de l’ADN, l’un des mécanismes épigénétiques les plus étudiés, peuvent être transmises par la lignée germinale aux les générations non-exposées (génération F3) suite à une exposition parentale (génération F0) externe aux radiations gamma (6,5 µGy.h-1 et 41,3 mGy.h-1) pendant 25 jours. Dans une seconde approche, un modèle mécaniste DEBtox (Budget Energétique Dynamique appliqué à la toxicologie) est modifié pour permettre l’analyse des effets des radiations gamma sur la croissance et la reproduction de D. magna à l’échelle de plusieurs générations. Pour ce faire, on utilise des compartiments de dommage, dont le niveau peut être hérité d’une génération à la suivante. Le modèle est ajusté aux données avec des méthodes d’inférence bayésienne afin d’estimer les paramètres tout en tenant compte des incertitudes qui leur sont associées.

In order to durably protect ecosystems facing planned or accidental releases of radionuclides, the long-term impact of organism exposure to ionizing radiation must be studied on a multigenerational scale. The aim of this PhD is to improve the characterization of molecular processes and the prediction of transgenerational effects during a gamma irradiation. First, an experimental approach investigated on radio-induced modifications of epigenetic processes, i.e. changes in mechanisms that regulate gene expression without changing DNA sequence itself and on the transmission of these modifications to subsequent generations. Significant changes in DNA methylation, a well-studied epigenetic mechanism, detected in generation F3 clearly showed that epigenetic modifications could be transmitted to unexposed generations, in response to the exposure of a parental generation (F0) to external gamma radiation (6.5 µGy.h-1 et 41.3 mGy.h-1) for 25 days. Second, a mechanistic modelling approach used a modified version of the DEBtox model (Dynamic Energy Budget model applied to toxicology) in order to analyze effects of gamma radiation on D. magna growth and reproduction over several generations. To that end, damage compartments, with damage levels that were transmitted from one generation to the next, were included. The model was fitted to data using Bayesian inference methods, in order to…

Advisors/Committee Members: Poggiale, Jean-Christophe (thesis director), Alonzo, Frédéric (thesis director).

Subjects/Keywords: Irradiation gamma; Effets transgénérationnels; Modifications épigénétiques; Méthylation de l’ADN; DEBtox; Daphnia magna; Gamma irradiation; Transgenerational effects; Epigenetic modification; DNA methylation; DEBtox; Daphnia magna; 550

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Trijau, M. (2018). Approche moléculaire et mécaniste de la réponse transgénérationnelle lors d'une irradiation gamma chronique chez le cladocère Daphnia magna : Molecular and mechanistic studies of the transgenerational response during a chronic gamma irradiation in the cladoceran Daphnia magna. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2018AIXM0467

Chicago Manual of Style (16^th Edition):

Trijau, Marie. “Approche moléculaire et mécaniste de la réponse transgénérationnelle lors d'une irradiation gamma chronique chez le cladocère Daphnia magna : Molecular and mechanistic studies of the transgenerational response during a chronic gamma irradiation in the cladoceran Daphnia magna.” 2018. Doctoral Dissertation, Aix Marseille Université. Accessed March 03, 2021. http://www.theses.fr/2018AIXM0467.

MLA Handbook (7^th Edition):

Trijau, Marie. “Approche moléculaire et mécaniste de la réponse transgénérationnelle lors d'une irradiation gamma chronique chez le cladocère Daphnia magna : Molecular and mechanistic studies of the transgenerational response during a chronic gamma irradiation in the cladoceran Daphnia magna.” 2018. Web. 03 Mar 2021.

Vancouver:

Trijau M. Approche moléculaire et mécaniste de la réponse transgénérationnelle lors d'une irradiation gamma chronique chez le cladocère Daphnia magna : Molecular and mechanistic studies of the transgenerational response during a chronic gamma irradiation in the cladoceran Daphnia magna. [Internet] [Doctoral dissertation]. Aix Marseille Université 2018. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2018AIXM0467.

Council of Science Editors:

Trijau M. Approche moléculaire et mécaniste de la réponse transgénérationnelle lors d'une irradiation gamma chronique chez le cladocère Daphnia magna : Molecular and mechanistic studies of the transgenerational response during a chronic gamma irradiation in the cladoceran Daphnia magna. [Doctoral Dissertation]. Aix Marseille Université 2018. Available from: http://www.theses.fr/2018AIXM0467

21. Parisot, Florian. Étude mécaniste des effets transgénérationnels des radiations ionisantes alpha et gamma chez Daphnia magna : Mechanistic study of transgenerational effects of alpha and gamma ionizing radiations in Daphnia magna.

Degree: Docteur es, Océanographie, 2015, Aix Marseille Université

URL: http://www.theses.fr/2015AIXM4104

►

Les activités anthropiques liées à l'industrie nucléaire contribuent à des rejets continus de radionucléides dans les écosystèmes terrestres et aquatiques. Les travaux réalisés au cours… (more)

▼

Les activités anthropiques liées à l'industrie nucléaire contribuent à des rejets continus de radionucléides dans les écosystèmes terrestres et aquatiques. Les travaux réalisés au cours de ce doctorat visent à apporter de nouvelles connaissances relatives aux effets des radiations ionisantes au cours d’une exposition multigénérationnelle de l’invertébré aquatique, Daphnia magna. Une irradiation gamma externe à des débits de doses pertinents du point de vue environnemental a été réalisée sur D. magna pendant trois générations successives. Les résultats mettent en évidence une accumulation et une transmission d’altérations de l’ADN au fil des générations, en parallèle d’une augmentation de la sensibilité des daphnies. Les données d’irradiation gamma et celles d’une étude antérieure de contamination alpha ont été analysées à l’aide du modèle mathématique DEBtox. Le modèle montre que les deux types de rayonnements agissent différemment sur les daphnies au cours des générations. Ce projet de recherche indique clairement qu’à l’avenir il est important d’étudier et de comprendre les effets transgénérationnels des radiations ionisantes à faibles doses.

Anthropogenic activities related to the nuclear industry contribute to continuous discharges of radionuclides into terrestrial and aquatic ecosystems. The aim of this PhD was to bring new knowledge on the effects of ionizing radiation during a multigenerational expose of the aquatic invertebrate, Daphnia magna. An external gamma radiation at environmentally relevant dose rates was performed on D. magna over three successive generations. Results show an accumulation and a transmission of DNA alterations over generations, in parallel of an increase in sensitivity of organisms. Gamma radiation data and those of a previous study of alpha contamination were analyzed using the mathematical model DEBtox. The model shows that the two types of radiation act differently on daphnia over generations. This research clearly indicates the importance of further studying and understanding transgenerational effects induced by low doses radiation in the future.

Advisors/Committee Members: Poggiale, Jean-Christophe (thesis director).

Subjects/Keywords: Daphnia magna; Irradiation gamma; Contamination alpha; Altérations de l'ADN; Effets transgénérationnels; DEBtox; Daphnia magna; Gamma radiation; Alpha contamination; DNA alterations; Transgenerational effects; DEBtox; 550

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parisot, F. (2015). Étude mécaniste des effets transgénérationnels des radiations ionisantes alpha et gamma chez Daphnia magna : Mechanistic study of transgenerational effects of alpha and gamma ionizing radiations in Daphnia magna. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2015AIXM4104

Chicago Manual of Style (16^th Edition):

Parisot, Florian. “Étude mécaniste des effets transgénérationnels des radiations ionisantes alpha et gamma chez Daphnia magna : Mechanistic study of transgenerational effects of alpha and gamma ionizing radiations in Daphnia magna.” 2015. Doctoral Dissertation, Aix Marseille Université. Accessed March 03, 2021. http://www.theses.fr/2015AIXM4104.

MLA Handbook (7^th Edition):

Parisot, Florian. “Étude mécaniste des effets transgénérationnels des radiations ionisantes alpha et gamma chez Daphnia magna : Mechanistic study of transgenerational effects of alpha and gamma ionizing radiations in Daphnia magna.” 2015. Web. 03 Mar 2021.

Vancouver:

Parisot F. Étude mécaniste des effets transgénérationnels des radiations ionisantes alpha et gamma chez Daphnia magna : Mechanistic study of transgenerational effects of alpha and gamma ionizing radiations in Daphnia magna. [Internet] [Doctoral dissertation]. Aix Marseille Université 2015. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2015AIXM4104.

Council of Science Editors:

Parisot F. Étude mécaniste des effets transgénérationnels des radiations ionisantes alpha et gamma chez Daphnia magna : Mechanistic study of transgenerational effects of alpha and gamma ionizing radiations in Daphnia magna. [Doctoral Dissertation]. Aix Marseille Université 2015. Available from: http://www.theses.fr/2015AIXM4104

Universitat Pompeu Fabra

22. Núñez Ollé, Marc, 1984-. The Role of cyclin O in the DNA damage response.

Degree: Departament de Ciències Experimentals i de la Salut, 2014, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/459302

► La Ciclina O és una nova ciclina que interacciona amb CDK1 i CDK2, i que s’ha demonstrat ser necessària per l’apoptòsi induïda per radiació gamma… (more)

Subjects/Keywords: Apoptosis; Càncer; Cicle cel·lular; Reparació de l'ADN; Dany a l'ADN; Senyal·lització cel·lular; Tumor; Hidrocefàlia; Radiació gamma; Resposta de dany a l'ADN; Recombinacuió homòloga; Fibroblast; Inestabilitat genòmica; Cancer; Cell cycle; DNA repair; DNA damage; Cell signaling; Tumour; Hydrocephalus; Gamma radiation; DNA damage response; Homologus recombination; Genomic instability; 577

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Núñez Ollé, Marc, 1. (2014). The Role of cyclin O in the DNA damage response. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/459302

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Núñez Ollé, Marc, 1984-. “The Role of cyclin O in the DNA damage response.” 2014. Thesis, Universitat Pompeu Fabra. Accessed March 03, 2021. http://hdl.handle.net/10803/459302.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Núñez Ollé, Marc, 1984-. “The Role of cyclin O in the DNA damage response.” 2014. Web. 03 Mar 2021.

Vancouver:

Núñez Ollé, Marc 1. The Role of cyclin O in the DNA damage response. [Internet] [Thesis]. Universitat Pompeu Fabra; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10803/459302.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Núñez Ollé, Marc 1. The Role of cyclin O in the DNA damage response. [Thesis]. Universitat Pompeu Fabra; 2014. Available from: http://hdl.handle.net/10803/459302

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Savva, Marilia. Ανάπτυξη και εφαρμογή τεχνικών προσδιορισμού πολύ χαμηλών συγκεντρώσεων ραδιενεργών ιχνοστοιχείων σε δείγματα περιβαλλοντικής σημασίας.

Degree: 2017, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ)

URL: http://hdl.handle.net/10442/hedi/42279

► The aim of this PhD Thesis was the determination of very low concentrations of natural and artificial radioactive isotopes in environmental samples by means of… (more)

▼ The aim of this PhD Thesis was the determination of very low concentrations of natural and artificial radioactive isotopes in environmental samples by means of gamma-spectroscopy techniques. Natural and artificial radioactive isotopes of low or higher specific activity are contained and can be detected in any environmental sample. Even when their specific activity is low, leading to negligible radiobiological significance, their detection is of interest in various applications, such as their use as tracers for environmental processes. In addition, improving the levels of detection of an isotope using a γ-spectroscopic apparatus, may allow the detection of the isotope in smaller volume samples without significant compromise on the accuracy of the measurement, which has obvious consequences in the design of the sampling. Since the background of a γ-spectroscopic apparatus is a factor determining the ability to detect low levels of radioactivity in a sample, its reduction is always highly desirable.In the framework of the Thesis the installation, study and use of the new Compton Suppression system (CSS), which was installed on the XtRa detector of the N.E.D. - N.T.U.A was carried out, aiming the reduction the continuous background of the detector and the improvement of the detection limits of the XtRa - CSS apparatus. The electronic set-up of the apparatus allows for the simultaneous collection of two spectra with a suppressed or not (unsuppressed) continuous background. In the framework of the study, a series of response factors were used, as referred in literature, through which the effect of various phenomena observed in the detection of photons by the Compton Suppression system was identified, and should be taken into account both during the calibration of the device, and during routine analyzes. As a result of this study, the optimal conditions for the detection of each isotope are proposed. The device was then calibrated experimentally but also using Monte Carlo simulation techniques for a series of source-to-detector geometries. For this purpose, it was necessary to modify the PENELOPE simulation code used in the N.E.D. - N.T.U.A in order to take into account both the true coincidence phenomenon during spectrum collection and to simulate a Compton Suppression system consisting of two independent detectors (primary detector and secondary detector or active shield) that communicate with each other.One of the main objectives of the Thesis was the detection of radioactive isotopes found at very low concentrations in environmental samples. For this purpose appropriate sampling techniques for atmospheric aerosols and liquid atmospheric precipitations and sample preparation were developed and a large number of samples were analyzed, mainly (a) contaminated samples due to the nuclear accidents in Chernobyl and Fukushima, (b) atmospheric aerosol and precipitation samples. From these analyzes it was possible to detect 241Am from the Chernobyl accident and a strong correlation with the 137Cs concentration was found. In…

Subjects/Keywords: γ-Φασματοσκοπία; Σύστημα Compton Suppression; Gamma-spectrometry; Compton Suppression System

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Savva, M. (2017). Ανάπτυξη και εφαρμογή τεχνικών προσδιορισμού πολύ χαμηλών συγκεντρώσεων ραδιενεργών ιχνοστοιχείων σε δείγματα περιβαλλοντικής σημασίας. (Thesis). National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Retrieved from http://hdl.handle.net/10442/hedi/42279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Savva, Marilia. “Ανάπτυξη και εφαρμογή τεχνικών προσδιορισμού πολύ χαμηλών συγκεντρώσεων ραδιενεργών ιχνοστοιχείων σε δείγματα περιβαλλοντικής σημασίας.” 2017. Thesis, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Accessed March 03, 2021. http://hdl.handle.net/10442/hedi/42279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Savva, Marilia. “Ανάπτυξη και εφαρμογή τεχνικών προσδιορισμού πολύ χαμηλών συγκεντρώσεων ραδιενεργών ιχνοστοιχείων σε δείγματα περιβαλλοντικής σημασίας.” 2017. Web. 03 Mar 2021.

Vancouver:

Savva M. Ανάπτυξη και εφαρμογή τεχνικών προσδιορισμού πολύ χαμηλών συγκεντρώσεων ραδιενεργών ιχνοστοιχείων σε δείγματα περιβαλλοντικής σημασίας. [Internet] [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10442/hedi/42279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Savva M. Ανάπτυξη και εφαρμογή τεχνικών προσδιορισμού πολύ χαμηλών συγκεντρώσεων ραδιενεργών ιχνοστοιχείων σε δείγματα περιβαλλοντικής σημασίας. [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2017. Available from: http://hdl.handle.net/10442/hedi/42279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Androulakaki, Effrosyni. Ανάπτυξη και υλοποίηση τεχνικών επιτόπιας φασματοσκοπίας-γ για εφαρμογές στο θαλάσσιο περιβάλλον.

Degree: 2016, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ)

URL: http://hdl.handle.net/10442/hedi/39397

► Gamma-Ray spectrometry is a method used to detect and quantify the concentrations of multiple radioactive elements in a single measurement, by collecting and discriminating in… (more)

▼ Gamma-Ray spectrometry is a method used to detect and quantify the concentrations of multiple radioactive elements in a single measurement, by collecting and discriminating in energy the emitted radiation, using suitable nuclear detectors. Radioactive elements (radionuclides) occur naturally on the earth's crust (primordial or terrestrial) or are produced in the upper atmosphere due to the interaction with cosmic rays (cosmogenic). Enhanced levels of radioactivity have been observed in several regions attributed to natural causes or to anthropogenic activities which are also responsible for the presence of a large variety of artificially produced radioactive elements in the environment. Radiation poses risks for living organisms by damaging tissues and the DNA and therefore the human population protection from radiation is one of the most significant applications of Gamma-Ray Spectrometry. Moreover, numerous radioactive elements constitute valuable tracers for a great variety of dynamic physical, chemical, geological and biological processes in soils, air, freshwater and oceans and therefore the knowledge of their concentration is essential. In situ gamma-ray measurements are nowadays increasingly substituting laboratory ones as they offer real-time continuous measurements and more representative results. My dissertation’s main contribution regarding in situ measurements on the aquatic environment was the development of a reliable and time-efficient method for the concentrations determination, using the Full Spectrum Analysis (FSA) technique. The methodology steps involved the implementation of Monte Carlo codes (MCNP-CP/FLUKA) to perform a theoretical calibration of the detector, the development of numerical algorithms for the radionuclide concentration estimations and the experimental validation of the theoretical results. This technique offers the advantage of a rapid determination of concentrations, which is crucial for handling large scale data or in cases where immediate decisions need to be taken (nuclear accident releases). It was applied in several different test cases by performing in situ measurements in different marine environments (Cyprus, Lavrion, and Peloponnesus). Concerning measurements on the seabed, the main objective of this thesis was to develop a methodology able to provide accurate concentration results. A substantial part of the analytical work is focused on understanding and exploiting a wide range of problems associated with in situ measurements on the seabed, where the available data are sparse. The parameters (morphology and sediment physical properties, geometry setups, inhomogeneous depth profiles) affecting the detection efficiency were determined, and their effects on the detector response were systematically studied, via MC simulations (MCNP5/FLUKA). As a result, a calibration approach for in situ gamma-ray measurements on the seabed was developed allowing the detection and determination of multiple radionuclide concentrations (in full spectral range). In addition,…

Subjects/Keywords: Φασματοσκοπία-γ; Monte Carlo simulations; In situ gamma-ray specroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Androulakaki, E. (2016). Ανάπτυξη και υλοποίηση τεχνικών επιτόπιας φασματοσκοπίας-γ για εφαρμογές στο θαλάσσιο περιβάλλον. (Thesis). National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Retrieved from http://hdl.handle.net/10442/hedi/39397

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Androulakaki, Effrosyni. “Ανάπτυξη και υλοποίηση τεχνικών επιτόπιας φασματοσκοπίας-γ για εφαρμογές στο θαλάσσιο περιβάλλον.” 2016. Thesis, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Accessed March 03, 2021. http://hdl.handle.net/10442/hedi/39397.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Androulakaki, Effrosyni. “Ανάπτυξη και υλοποίηση τεχνικών επιτόπιας φασματοσκοπίας-γ για εφαρμογές στο θαλάσσιο περιβάλλον.” 2016. Web. 03 Mar 2021.

Vancouver:

Androulakaki E. Ανάπτυξη και υλοποίηση τεχνικών επιτόπιας φασματοσκοπίας-γ για εφαρμογές στο θαλάσσιο περιβάλλον. [Internet] [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10442/hedi/39397.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Androulakaki E. Ανάπτυξη και υλοποίηση τεχνικών επιτόπιας φασματοσκοπίας-γ για εφαρμογές στο θαλάσσιο περιβάλλον. [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2016. Available from: http://hdl.handle.net/10442/hedi/39397

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Wayne State University

25. Astapova, Olga. Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes.

Degree: PhD, Pathology, 2012, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/631

► PPAR-gamma is a nuclear receptor that plays a central role in metabolic regulation by regulating extensive gene expression networks in adipose, liver, skeletal muscle… (more)

▼ PPAR-gamma is a nuclear receptor that plays a central role in metabolic regulation by regulating extensive gene expression networks in adipose, liver, skeletal muscle and many other tissues. Human PPAR-gamma mutations are rare and cause a monogenetic form of severe type II diabetes with metabolic syndrome, known as familiar partial lypodystrophy. The E157D PPAR-gamma mutant causes atypical lipodystrophy in a large Canadian kindred, presenting with multiple musculoskeletal, neurological and hematological abnormalities in addition to the classic lipodystrophy features of insulin-resistant diabetes, hypertension and dyslipidemia. This mutation is localized to the p-box of PPAR-gamma, a small region that interacts directly with the DNA molecule and is required for DNA binding site specificity. Mechanistic analysis revealed that E157D PPAR-gamma binds PPAR-gamma response elements (PPREs), but is mildly, moderately or severely defective at inducing transcription from most promoters, without dominant negative activity. This suppression of transcriptional activity may be mediated by an increased effect of nuclear receptor corepressors on E157D PPAR-gamma. In addition, the mutant binds atypical PPREs in the regulatory regions of a small set of genes outside of the PPAR-gamma-regulated network, and induces transcription of these genes. The loss of transcriptional activity on PPAR-gamma-regulated promoters leads to the metabolic disease in the E157D PPAR-gamma cohort, while the gain of activity on non- PPAR-gamma target promoters may explain the atypical clinical presentation associated with this mutation. The misregulation of target genes by this DNA-contacting mutant highlights a previously under-appreciated importance of the DNA molecule as an allosteric regulator of the transcriptional activity of nuclear receptors. In summary, this dissertation describes a human PPAR-gamma mutation that works through a novel mechanism to cause atypical lipodystrophy, and provides support for a more integrated view of the nuclear receptor-DNA interaction and transcription activation. Advisors/Committee Members: Todd A. Leff.

Subjects/Keywords: DNA binding; gene regulation; metabolic syndrome; nuclear receptors; PPAR-gamma; transcription coregulators; Bioinformatics; Genetics; Molecular Biology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Astapova, O. (2012). Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/631

Chicago Manual of Style (16^th Edition):

Astapova, Olga. “Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes.” 2012. Doctoral Dissertation, Wayne State University. Accessed March 03, 2021. https://digitalcommons.wayne.edu/oa_dissertations/631.

MLA Handbook (7^th Edition):

Astapova, Olga. “Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes.” 2012. Web. 03 Mar 2021.

Vancouver:

Astapova O. Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes. [Internet] [Doctoral dissertation]. Wayne State University; 2012. [cited 2021 Mar 03]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/631.

Council of Science Editors:

Astapova O. Mechanistic Studies Of A Novel Ppar-Gamma Mutant That Causes Lipodystrophy And Diabetes. [Doctoral Dissertation]. Wayne State University; 2012. Available from: https://digitalcommons.wayne.edu/oa_dissertations/631

University of Southern California

26. Fouch, Brenda L. Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions.

Degree: MS, Geological Sciences, 2008, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/60237/rec/5830

► In understanding DNA as a biomarker over geologic time scales, it is important to determine the longevity of the signal under common environmental stress. Shewanella… (more)

Subjects/Keywords: DNA signal longevity; rock record; biomarker; gamma; UVC; cyrogenic

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fouch, B. L. (2008). Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/60237/rec/5830

Chicago Manual of Style (16^th Edition):

Fouch, Brenda L. “Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions.” 2008. Masters Thesis, University of Southern California. Accessed March 03, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/60237/rec/5830.

MLA Handbook (7^th Edition):

Fouch, Brenda L. “Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions.” 2008. Web. 03 Mar 2021.

Vancouver:

Fouch BL. Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions. [Internet] [Masters thesis]. University of Southern California; 2008. [cited 2021 Mar 03]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/60237/rec/5830.

Council of Science Editors:

Fouch BL. Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions. [Masters Thesis]. University of Southern California; 2008. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/60237/rec/5830

University of Texas – Austin

27. Estep, Patricia Ann. The role of residue Y955 of mitochondrial DNA polymerase [gamma] in nucleotide binding and discrimination.

Degree: MA, Biochemistry, 2010, University of Texas – Austin

URL: http://hdl.handle.net/2152/ETD-UT-2010-12-2395

► The human mitochondrial polymerase (pol γ) is a nuclearly-encoded polymerase that is solely responsible for the faithful replication and repair of the mitochondrial genome. The… (more)

Subjects/Keywords: Pre-steady state kinetics; DNA polymerase gamma

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Estep, P. A. (2010). The role of residue Y955 of mitochondrial DNA polymerase [gamma] in nucleotide binding and discrimination. (Masters Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2010-12-2395

Chicago Manual of Style (16^th Edition):

Estep, Patricia Ann. “The role of residue Y955 of mitochondrial DNA polymerase [gamma] in nucleotide binding and discrimination.” 2010. Masters Thesis, University of Texas – Austin. Accessed March 03, 2021. http://hdl.handle.net/2152/ETD-UT-2010-12-2395.

MLA Handbook (7^th Edition):

Estep, Patricia Ann. “The role of residue Y955 of mitochondrial DNA polymerase [gamma] in nucleotide binding and discrimination.” 2010. Web. 03 Mar 2021.

Vancouver:

Estep PA. The role of residue Y955 of mitochondrial DNA polymerase [gamma] in nucleotide binding and discrimination. [Internet] [Masters thesis]. University of Texas – Austin; 2010. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152/ETD-UT-2010-12-2395.

Council of Science Editors:

Estep PA. The role of residue Y955 of mitochondrial DNA polymerase [gamma] in nucleotide binding and discrimination. [Masters Thesis]. University of Texas – Austin; 2010. Available from: http://hdl.handle.net/2152/ETD-UT-2010-12-2395

28. ΑΘΑΝΑΣΙΟΥ, ΚΥΡΙΑΚΟΣ. ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΩΝ ΕΠΙΔΙΟΡΘΩΤΙΚΩΝ ΜΗΧΑΝΙΣΜΩΝ ΤΟΥ DNA ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΑΤΟΜΩΝΜΕ ΣΥΝΔΡΟΜΟ DOWN.

Degree: 1981, University of Patras; Πανεπιστήμιο Πατρών

URL: http://hdl.handle.net/10442/hedi/0814

►

THE CAPABILITY OF CELLS FROM CHILDREN WITH DOWN'S SYNDROME TO REPAIR X-RAY INDUCED DAMAGE IN DNA WAS STUDIED BY MEANS OF THREE DIFFERENT METHODS, NAMELY:… (more)

▼

THE CAPABILITY OF CELLS FROM CHILDREN WITH DOWN'S SYNDROME TO REPAIR X-RAY INDUCED DAMAGE IN DNA WAS STUDIED BY MEANS OF THREE DIFFERENT METHODS, NAMELY: 1) BY COUNTING SISTER CHROMATID EXCHANGES (SCE) IN NORMAL AND DOWN CELLS WHICH WEREFOUND OF SIMILAR LEVELS. IN THIS RESPECT DS RESEMBLES XERODERMA PIGMENTOSUM. 2) BY STUDYING THE DNA SEDIMENTATION PROFILES IN ALKALINE SUCROSE GRADIENTS WHICH SHOWED A LESS EFFICIENT CAPACITY OF DS LYMPHOCYTES TO REPAIR SINGLE STRAND BREAKS INDUCED BY X- RAYS. 3) BY COMPARING THE FREQUENCY OF ASYMMETRIC TO SYMMETRIC CHROMATID INTERCHANGES. THE INCREASED FREQUENCY OF INDUCED ASYMMETRIC INTERCHANGES DETECTED IN DS WAS ATTRIBUTED TO AN ALTERED WAY OF ACTION OF THE REPAIR MECHANISM OF THESE CELLS COMPARED TO THAT OF THE NORMALS.

Η ΔΙΑΤΡΙΒΗ ΑΝΑΦΕΡΕΤΑΙ ΣΤΟ ΣΥΝΔΡΟΜΟ DOWN ΠΟΥ ΟΦΕΙΛΕΤΑΙ ΣΕ ΜΙΑ ΧΡΩΜΟΣΩΜΙΚΗ ΑΝΩΜΑΛΙΑ Η ΣΥΧΝΟΤΗΤΑ ΤΗΣ ΟΠΟΙΑΣ ΑΥΞΑΝΕΙ ΜΕ ΤΗΝ ΗΛΙΚΙΑ ΤΗΣ ΜΗΤΕΡΑΣ. Η ΔΙΑΠΙΣΤΩΣΗ ΠΩΣ ΤΑ ΛΕΜΦΟΚΥΤΤΑΡΑ ΤΩΝ ΠΑΣΧΟΝΤΩΝ ΠΑΡΟΥΣΙΑΖΟΥΝ ΔΙΠΛΑΣΙΑ ΕΥΑΙΣΘΗΣΙΑ ΟΣΟΝ ΑΦΟΡΑ ΤΗ ΔΗΜΙΟΥΡΓΙΑ ΧΡΩΜΑΤΟΣΩΜΙΚΩΝ ΜΕΤΑΛΛΑΞΕΩΝ, ΜΕΤΑ ΑΠΟ ΕΚΘΕΣΗ ΣΕ ΑΚΤΙΝΕΣ Χ ΟΔΗΓΗΣΕ ΣΤΗΝ ΥΠΟΘΕΣΗ ΠΩΣ ΣΤΑ ΑΤΟΜΑ ΑΥΤΑ ΕΙΝΑΙ ΔΥΝΑΤΟ ΝΑ ΥΠΑΡΧΕΙ ΚΑΠΟΙΑ ΒΛΑΒΗ ΣΤΟ ΣΥΣΤΗΜΑ ΠΟΥ ΕΠΙΔΙΟΡΘΩΝΕΙ ΤΙΣ ΓΕΝΕΤΙΚΕΣ ΒΛΑΒΕΣ. Η ΥΠΟΘΕΣΗ ΕΞΕΤΑΣΘΗΚΕ ΜΕ ΤΡΕΙΣ ΔΙΑΦΟΡΕΤΙΚΟΥΣ ΤΡΟΠΟΥΣ. 1) ΜΕ ΣΥΓΚΡΙΣΗ ΤΩΝ ΣΥΧΝΟΤΗΤΩΝ ΤΩΝ ΤΥΧΑΙΩΝ ΑΝΤΑΛΛΑΓΩΝ ΣΤΑ ΑΔΕΛΦΑ ΧΡΩΜΑΤΙΔΙΑ ΚΑΝΟΝΙΚΩΝ ΚΑΙ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΝΔΡΟΜΟ DOWN (SCE) ΧΩΡΙΣ ΝΑ ΒΡΕΘΕΙ ΣΗΜΑΝΤΙΚΗ ΔΙΑΦΟΡΑ. ΑΠΟ ΤΗΝ ΑΠΟΨΗ ΑΥΤΗ ΤΟ ΣΥΝΔΡΟΜΟ DOWN ΜΟΙΑΖΕΙ ΜΕ ΤΟ ΣΥΝΔΡΟΜΟ ΤΗΣ ΜΕΛΑΓΧΡΩΜΑΤΙΚΗΣ ΞΗΡΟΔΕΡΜΙΑΣ. 2) ΜΕΛΕΤΗΘΗΚΕ Η ΙΚΑΝΟΤΗΤΑ ΤΩΝ ΚΥΤΤΑΡΩΝ DOWN ΝΑ ΕΠΙΔΙΟΡΘΩΝΟΥΝ ΒΛΑΒΕΣ ΠΡΟΚΑΛΟΥΜΕΝΕ ΜΕ ΑΚΤΙΝΟΒΟΛΙΑ Γ ΔΗΜΙΟΥΡΓΩΝΤΑΣ ΚΛΙΜΑΚΕΣ ΚΑΤΑΝΟΜΗΣ ΣΕ ΒΑΘΜΙΔΩΣΕΙΣ ΑΛΚΑΛΙΚΗΣ ΣΑΚΧΑΡΟΖΗΣ. ΣΤΟΝ ΚΥΚΛΟ ΑΥΤΟ ΤΩΝ ΠΕΙΡΑΜΑΤΩΝ ΔΙΑΦΑΝΗΚΕ ΜΙΑ ΕΛΛΑΤΩΜΕΝΗ ΙΚΑΝΟΤΗΤΑ ΣΤΑ ΚΥΤΤΑΡΑ DOWN ΝΑ ΕΠΙΔΙΟΡΘΩΝΟΥΝ ΜΟΝΑ ΡΗΓΜΑΤΑ ΠΟΥ ΕΠΑΓΟΝΤΑΙ ΣΤΟ DNA ΜΕ ΑΚΤΙΝΟΒΟΛΙΑ Γ. 3) ΕΓΙΝΕ ΣΥΓΚΡΙΣΗ ΤΗΣ ΣΥΧΝΟΤΗΤΑΣ ΤΩΝ ΑΣΥΜΜΕΤΡΙΚΩΝ ΩΣ ΠΡΟΣ ΤΙΣ ΣΥΜΜΕΤΡΙΚΕΣ ΧΡΩΜΑΤΙΔΙΑΚΕΣ ΑΝΤΑΛΛΑΓΕΣ ΠΟΥ ΟΦΕΙΛΟΝΤΑΙ ΣΕ ΑΚΤΙΝΟΒΟΛΙΑ Γ. Η ΥΠΕΡΟΧΗ ΤΩΝ ΑΣΥΜΜΕΤΡΙΚΩΝ ΣΤΟ ΣΥΝΔΡΟΜΟ DOWN ΑΠΟΔΟΘΗΚΕ ΣΕ ΕΝΑ ΤΡΟΠΟ ΔΡΑΣΗΣ ΤΟΥ ΕΠΙΔΙΟΡΘΩΤΙΚΟΥ ΜΗΧΑΝΙΣΜΟΥ ΔΙΑΦΟΡΕΤΙΚΟΥ ΑΠΟ ΕΚΕΙΝΟΥ ΠΟΥ ΑΠΑΝΤΑ ΣΤΑ ΚΑΝΟΝΙΚΑ ΚΥΤΤΑΡΑ.

Subjects/Keywords: Ακτινοβολία - γ; ΑΝΤΑΛΛΑΓΕΣ ΑΔΕΛΦΙΚΩΝ ΧΡΩΜΑΤΙΔΙΩΝ; Βλάβες DNA; Επιδιόρθωση; ΣΥΜΜΕΤΡΙΚΕΣ-ΑΣΥΜΜΕΤΡΙΚΕΣ ΕΞΩΑΝΤΑΛΛΑΓΕΣ; Σύνδρομο Down; Χρωμοσώματα; Χρωμοσωματικές μεταλλάξεις; Chromosome aberrations; Chromosomes; DNA damage; DNA-REPAIR; Down syndrome; Sister chromatid exchanges; SYMMETRICAL-ASSYMETRICAL INTERCHANGES; Γ-RADIATION

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ΑΘΑΝΑΣΙΟΥ, . �. (1981). ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΩΝ ΕΠΙΔΙΟΡΘΩΤΙΚΩΝ ΜΗΧΑΝΙΣΜΩΝ ΤΟΥ DNA ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΑΤΟΜΩΝΜΕ ΣΥΝΔΡΟΜΟ DOWN. (Thesis). University of Patras; Πανεπιστήμιο Πατρών. Retrieved from http://hdl.handle.net/10442/hedi/0814

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ΑΘΑΝΑΣΙΟΥ, ΚΥΡΙΑΚΟΣ. “ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΩΝ ΕΠΙΔΙΟΡΘΩΤΙΚΩΝ ΜΗΧΑΝΙΣΜΩΝ ΤΟΥ DNA ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΑΤΟΜΩΝΜΕ ΣΥΝΔΡΟΜΟ DOWN.” 1981. Thesis, University of Patras; Πανεπιστήμιο Πατρών. Accessed March 03, 2021. http://hdl.handle.net/10442/hedi/0814.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ΑΘΑΝΑΣΙΟΥ, ΚΥΡΙΑΚΟΣ. “ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΩΝ ΕΠΙΔΙΟΡΘΩΤΙΚΩΝ ΜΗΧΑΝΙΣΜΩΝ ΤΟΥ DNA ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΑΤΟΜΩΝΜΕ ΣΥΝΔΡΟΜΟ DOWN.” 1981. Web. 03 Mar 2021.

Vancouver:

ΑΘΑΝΑΣΙΟΥ �. ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΩΝ ΕΠΙΔΙΟΡΘΩΤΙΚΩΝ ΜΗΧΑΝΙΣΜΩΝ ΤΟΥ DNA ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΑΤΟΜΩΝΜΕ ΣΥΝΔΡΟΜΟ DOWN. [Internet] [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 1981. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10442/hedi/0814.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ΑΘΑΝΑΣΙΟΥ �. ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΩΝ ΕΠΙΔΙΟΡΘΩΤΙΚΩΝ ΜΗΧΑΝΙΣΜΩΝ ΤΟΥ DNA ΣΕ ΛΕΜΦΟΚΥΤΤΑΡΑ ΑΤΟΜΩΝΜΕ ΣΥΝΔΡΟΜΟ DOWN. [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 1981. Available from: http://hdl.handle.net/10442/hedi/0814

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Moron, Michelle Mendes. Efeito da ação combinada de radiação gama e campo elétrico estático em células humanas.

Degree: Mestrado, Biotecnologia, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21012009-095743/ ;

►

Neste trabalho estudamos o efeito da exposição de células humanas à radiação ionizante e em associação a campos elétricos exógenos estáticos. A linhagem T47D de… (more)

▼

Neste trabalho estudamos o efeito da exposição de células humanas à radiação ionizante e em associação a campos elétricos exógenos estáticos. A linhagem T47D de células de carcinoma ductal mamário foi irradiada com gamas no intervalo 0 8 Gy. A viabilidade celular da linhagem T47D exposta à radiação gama e campo elétrico estático (CEE) de 1.250 V/cm foi cerca de 12% inferior à viabilidade observada apenas com irradiação. Quando aplicado isoladamente por 24 e 72 horas o CEE não induziu toxicidade. A imunofluorescência realizada na linhagem normal MRC5 (fibroblasto de pulmão humano normal) quantificou a expressão da histona -H2AX. A quantidade de histonas fosforiladas foi cerca de 40% maior após irradiação com 2 Gy mais CEE aplicado por 1h, indicando que o campo elétrico interferiu negativamente no processo de reparo das quebras duplas de DNA. A análise de citometria de fluxo (FACS) mostrou que em células T47D tratadas com 1 e 2 Gy por 24 horas o CEE também interferiu negativamente no processo de reparo do DNA, notadamente pelo maior acúmulo de células na fase S.

Our goal is the study in human cells of the effect resulting from the association of irradiation with exposure to exogenous static electric fields. The T47D cell line of breast cancer cells was irradiated with gammas in the 0 8 Gy doses range. The viability of this T47D cells exposed to both gamma radiation and 1.250 V/cm static electric field (SEF) was about 12% lower than when only irradiated. The sole exposure of the cells to SEF by 24 and 72 hours didnt induce toxicity. Immunofluorescence runs carried out in irradiated normal MRC5 cell line of human lung fibroblast have quantified the expression of the g-H2AX histone. The amount of phosphorylated histones was approximately 40% higher after irradiation with 2 Gy plus exposure to a SEF by 1 hour, showing that the electric field negatively interfered in the repairing process of the DNA double strand breaks. The flow cytometry analysis with FACS showed that in T47D cells treated with 1 and 2 Gy by 24 hours the SEF also negatively interfered in the DNA repairing process, as evidenced by the higher accumulation of cells in the S phase.

Advisors/Committee Members: Arruda Neto, Joao Dias de Toledo, Segreto, Helena Regina Comodo.

Subjects/Keywords: Cell cycle; Ciclo celular; Curvas de sobrevivência; DNA repair; Gamma radiation; Radiação gama; Radiobiology; Radiologia; Radioterapia; Radiotherapy; Reparo de DNA; Survival curve

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moron, M. M. (2008). Efeito da ação combinada de radiação gama e campo elétrico estático em células humanas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21012009-095743/ ;

Chicago Manual of Style (16^th Edition):

Moron, Michelle Mendes. “Efeito da ação combinada de radiação gama e campo elétrico estático em células humanas.” 2008. Masters Thesis, University of São Paulo. Accessed March 03, 2021. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21012009-095743/ ;.

MLA Handbook (7^th Edition):

Moron, Michelle Mendes. “Efeito da ação combinada de radiação gama e campo elétrico estático em células humanas.” 2008. Web. 03 Mar 2021.

Vancouver:

Moron MM. Efeito da ação combinada de radiação gama e campo elétrico estático em células humanas. [Internet] [Masters thesis]. University of São Paulo; 2008. [cited 2021 Mar 03]. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21012009-095743/ ;.

Council of Science Editors:

Moron MM. Efeito da ação combinada de radiação gama e campo elétrico estático em células humanas. [Masters Thesis]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-21012009-095743/ ;

University of Helsinki

30. Kukkonen, Sami. The RNA polymerase of Tula virus.

Degree: 1996, University of Helsinki

URL: http://hdl.handle.net/10138/157264

Subjects/Keywords: Bunyaviridae; hantavirus; Tula virus; L RNA; RNA-polymeraasi; Biokemia; Bunyaviridae; hantavirus; Tula virus; L RNA; RNA-polymeraasi

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kukkonen, S. (1996). The RNA polymerase of Tula virus. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/157264

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kukkonen, Sami. “The RNA polymerase of Tula virus.” 1996. Thesis, University of Helsinki. Accessed March 03, 2021. http://hdl.handle.net/10138/157264.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kukkonen, Sami. “The RNA polymerase of Tula virus.” 1996. Web. 03 Mar 2021.

Vancouver:

Kukkonen S. The RNA polymerase of Tula virus. [Internet] [Thesis]. University of Helsinki; 1996. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10138/157264.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kukkonen S. The RNA polymerase of Tula virus. [Thesis]. University of Helsinki; 1996. Available from: http://hdl.handle.net/10138/157264

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations