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You searched for subject:(DENV 2). Showing records 1 – 2 of 2 total matches.

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NSYSU

1. Lin, Chun-kuang. Development of antiviral drugs from marine natural products and investigation of drug target against virus.

Degree: PhD, Doctoral Degree Program in Marine Biotechnology, 2018, NSYSU

Hepatitis C virus (HCV) infection causes chronic inflammation of liver, leading to the development of cirrhosis and hepatocellular carcinoma (HCC). Infection of dengue virus (DENV) caused diseases ranging from acute self-limiting febrile illness to life-threatening dengue hemorrhagic fever and dengue shock syndrome. The purposes of present dissertation are to discover the anti-viral agents from marine natural products and to investigate the impact of cellular factors on DENV replication. For finding the potential antivirals, we found that betulinic acid (BA) and acteoside (AM-4) extracted from Avicennia marina could reduce HCV replication. The mechanism study demonstrated that BA reduced HCV replication through decreasing the NF-κB- and ERK1/2-mediated cyclooxygenase-2 (COX-2) expression. The AM-4 suppressed HCV infection by blocking viral entry into cells and cell-to-cell spread of HCV. In addition, we identified that lobohedleolide extracted from soft coral exhibited anti-HCV activity by suppression of HCV-induced COX-2 expression. Using various COX-2 promoter deletion constructs linked to luciferase reporter gene, we first identified CCAAT/enhancer-binding protein (C/EBP) as a key transcription factor for the down-regulation of COX-2 by lobohedleolide, and then demonstrated that the HCV-induced C/EBP expression could be suppressed by lobohedleolide through inhibiting the phosphorylation of JNK and c-Jun. Notably, combination treatment of BA, AM-4 and lobohedleolide with several clinically used HCV drugs synergistically inhibited HCV RNA replication, indicating that these three natural products exhibited a high biomedical potential to be used as a supplementary agent for control of HCV infection. Besides, BA and lobohedleolide also exhibited anti-DENV activity. For finding the therapeutic targets from cellular gene against DENV, we observed an increased level of COX-2 in patients with dengue fever compared with healthy individuals. Then, an elevated level of COX-2 expression was also observed in DENV-infected ICR suckling mice. COX-2 gene silencing and catalytic inhibition sufficiently suppressed DENV-2 replication. Using ICR suckling mouse model, we identified that the COX-2 inhibitor NS398 protected mice from succumbing to life-threatening DENV-2 infection, revealing targeting COX-2 is a promising strategy to control DENV infection. In addition, we found that the expression of prostasin, a serine protease, is lower in patients with dengue fever than in healthy individuals. Exogenous expression of prostasin could protect ICR suckling mice from life-threatening DENV-2 infection and reduce DENV-2 propagation in Huh-7 cells. We further revealed that prostasin reduced DENV replication through proteolytic cleavage of epithelial growth factor receptor (EGFR). The activity of proteolytic cleavage of prostasin is dependent on the expression of matriptase and hepatocyte growth factor activator inhibitor type 2 (HAI-2). Collectively, COX-2 and prostasin exhibited highly potential to serve as therapeutic targets against… Advisors/Committee Members: Jyh-Horng Sheu (chair), Jin-Ching Lee (chair), Shih-Hsiung Wu (chair), Chih-Chuang Liaw (committee member), Yen-Hsu Chen (chair).

Subjects/Keywords: DENV; COX-2; marine natural product; EGFR; prostasin; HCV

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lin, C. (2018). Development of antiviral drugs from marine natural products and investigation of drug target against virus. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352

Chicago Manual of Style (16th Edition):

Lin, Chun-kuang. “Development of antiviral drugs from marine natural products and investigation of drug target against virus.” 2018. Doctoral Dissertation, NSYSU. Accessed November 23, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352.

MLA Handbook (7th Edition):

Lin, Chun-kuang. “Development of antiviral drugs from marine natural products and investigation of drug target against virus.” 2018. Web. 23 Nov 2020.

Vancouver:

Lin C. Development of antiviral drugs from marine natural products and investigation of drug target against virus. [Internet] [Doctoral dissertation]. NSYSU; 2018. [cited 2020 Nov 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352.

Council of Science Editors:

Lin C. Development of antiviral drugs from marine natural products and investigation of drug target against virus. [Doctoral Dissertation]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352

2. Shanks, Melissa Christine. Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway.

Degree: School of Chemistry and Molecular Biosciences, 2015, University of Queensland

Subjects/Keywords: DENV-2; Dengue fever; Flavivirus; Virus Fusion; Structural Proteins; Envelope Proteins; 030406 Proteins and Peptides; 0601 Biochemistry and Cell Biology; 060108 Protein Trafficking

…70 83 99 108 12 Abbreviations: ADE bp C C6/36 CDC CO2 CPE CS DENV-2 DF DHF dH2O DI,II… …x5B;8]. In February 2011, there were 39 cases of DENV-2 in East Innisfail, QLD [9… …x5D; (figure 11). In DENV-2, E2 contains four phenylalanine and one tyrosine… …pCI_NGCprME_opt_neo (New Guinea DENV 2, optimized, neomyocin). The vector contains the prME genes… …CHAPTER 2 MATERIALS and METHODS 2.1. Vectors 2.1.1. pCI_NGCprME_opt_neo 2.1.2. pSFV and… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Shanks, M. C. (2015). Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:363952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Shanks, Melissa Christine. “Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway.” 2015. Thesis, University of Queensland. Accessed November 23, 2020. http://espace.library.uq.edu.au/view/UQ:363952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Shanks, Melissa Christine. “Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway.” 2015. Web. 23 Nov 2020.

Vancouver:

Shanks MC. Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway. [Internet] [Thesis]. University of Queensland; 2015. [cited 2020 Nov 23]. Available from: http://espace.library.uq.edu.au/view/UQ:363952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shanks MC. Site-directed mutagenesis of the membrane-proximal (MPER) α-helical E2 region of DENV E to characterise the significance of individual residues in protein expression and migration through the secretory pathway. [Thesis]. University of Queensland; 2015. Available from: http://espace.library.uq.edu.au/view/UQ:363952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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