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You searched for subject:(D abyssinica). Showing records 1 – 2 of 2 total matches.

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Addis Ababa University

1. Belay, Tadesse. In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia .

Degree: 2014, Addis Ababa University

In vitro and in vivo anti-trypanosomal activity of dichloromethane and methanol crude leaf extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense field isolate Belay Tadesse Addis Ababa University, 2014 African Trypanosomosis is a neglected tropical disease of medical and veterinary importance. Parasite control relies on the use of few drugs whose resistance and unacceptable toxicities urged the investigation of new agents, preferably from natural sources. Though Dovyalis abyssinica has been reported to posses significant trypanocidal activity on Trypanosoma brucei in in vitro model, activity on trypanosome infected laboratory animals has not yet been worked out. The aim of the present study was, therefore, to investigate the in vitro and in vivo activity of D. abyssinica on T. congolense field isolate. To evaluate the effect on motility, 200 μl T. congolense infected blood was mixed with 50 μl of 20, 10, 2, 0.1, 0.015 mg/ml dichloromethane and methanol extracts. Reduction or cessation of motility was then microscopically monitored for 120 minutes, and the remaining in vitro mixtures were inoculated to healthy mice and monitored for development of infection for 21 days. Furthermore, fifty T. congolense infected mice were randomly grouped into ten groups of five and administered with curative doses (250, 200, 150 and 100 mg/kg) of dichloromethane and methanol and 28 mg/kg diminazene aceturate and dimethylsulfoxide. Following administration, parasitemia, packed cell volume (PCV), rectal temperature, body weight and survival time were iii monitored. Suppressive doses of the extracts (250 and 200 mg/kg) were administered 24 hours post-infection and parasitemia was monitored. Dichloromethane and methanol extracts at 20, 10, 2 mg/ml concentrations ceased parasite motility within one hour and eliminated subsequent infectivity in mice for 21 days. Administration of dichloromethane and methanol extracts at 250 and 200 mg/kg reduced (p<0.05) parasitemia and rectal temperature, and improved (p<0.05) PCV, mean body weight, and mean survival time compared to DMSO treatment. In conclusion, D. abyssinica at higher concentrations in vitro and higher curative doses in vivo in mice posses anti-trypanosomal activity. Keywords: Anti-trypanosomal, D. abyssinica, in vitro, in vivo, T. congolense, mice. Advisors/Committee Members: Getachew Abebe(Prof) (advisor).

Subjects/Keywords: Anti-trypanosomal; D. abyssinica; in vitro; in vivo; T. congolense; mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Belay, T. (2014). In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Belay, Tadesse. “In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia .” 2014. Thesis, Addis Ababa University. Accessed December 09, 2019. http://etd.aau.edu.et/dspace/handle/123456789/6072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Belay, Tadesse. “In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia .” 2014. Web. 09 Dec 2019.

Vancouver:

Belay T. In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia . [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2019 Dec 09]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belay T. In Vitro and In Vivo Anti-trypanosomal Activity of Dichloromethane and Methanol Crude Leaf Extracts of Dovyalis abyssinica (Flacourtiaceae) against Trypanosoma congolense Field Isolate Belay Tadesse A Thesis Submitted to the Department of Pharmacology and Clinical Pharmacy Presented in Partial Fulfillment of the Requirements for the Degree of Master of Science (Pharmacology) Addis Ababa University Addis Ababa, Ethiopia . [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Addis Ababa University

2. Bilal, Tessema. PHYSICOCHEMICAL CHARACTERIZATION OF ACID MODIFIED DIOSCOREA STARCH AND ITS EVALUATION AS DIRECTLY COMPRESSIBLE EXCIPIENT AND DISINTEGRANT IN TABLET FORMULATIONS .

Degree: 2014, Addis Ababa University

Starch is one of the most important and flexible food ingredients possessing value added attributes for innumerable industrial applications. Dioscorea abyssinica (Fam. Dioscoreaceae) is a potential source of starch for large scale production in Ethiopia. Starch is usually modified either chemically or physically to augment its convenience for industrial use. The choice of excipients remains a critical factor in pharmaceutical formulations. Acid modified dioscorea starch (AMDS) has been prepared by hydrolyzing with 6% (w/w) HCl solution at ambient conditions and evaluated as a multifunctional excipient i.e, its direct compressibility and disintegrant effect in tablet formulations. Some of the physicochemical properties of AMDS such as; densities, flow, swelling power, percentage solubility, moisture sorption, and compactability were determined and compared to those of native dioscorea starch (NDS) and Starch 1500®. The comparative direct compressibility and disintegrant effects of these starches in tablet formulations were also studied using paracetamol as a model drug and the resulting tablets were evaluated for their physico-mechanical properties. The results indicated that both swelling power and percent solubility of the starches were found to increase with increase in temperature; AMDS showed higher percent solubility 68.53%. Spray dried AMDS exhibited free flowing property with 21.37 ± 1.39° angle of repose and 13.24 ± 0.76 g/sec flow rate. The compaction study revealed that the tensile strength and disintegration time of the compact increases and percent friability decreases as compression force increases. The lubricant sensitivity test showed that spray dried AMDS and Starch 1500® tablets could be used with acceptable tablet characteristics up to 1% and 0.5% of magnesium stearate concentration, respectively. With regard to dilution potential, the spray dried AMDS could accommodate up to 40% paracetamol with acceptable criteria. The results also indicated that tablets prepared with AMDS and Starch 1500® exhibited comparable characteristics. An increase in concentration of AMDS and Starch 1500® as disintegrant provided tablets with increased crushing strength and decreased disintegration time and friability. The dissolution studies of the paracetamol tablets showed that the release of the drug was within acceptable range (quantity dissolved in 30 min ≥ 80%). Thus, it can be concluded that AMDS can be used as an alternative directly compressible excipient and disintegrant in tablet formulations. Keywords: Dioscorea starch; acid modified starch; spray drying; tablet; directly compressible excipient; disintegrant; lubricant sensitivity, dilution potential, D. abyssinica. Advisors/Committee Members: Tsige Gebre-Mariam(Prof.). Anteneh Belete( Dr) (advisor).

Subjects/Keywords: Dioscorea starch; acid modified starch; spray drying; tablet; directly compressible excipient; disintegrant; lubricant sensitivity, dilution potential, D. abyssinica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bilal, T. (2014). PHYSICOCHEMICAL CHARACTERIZATION OF ACID MODIFIED DIOSCOREA STARCH AND ITS EVALUATION AS DIRECTLY COMPRESSIBLE EXCIPIENT AND DISINTEGRANT IN TABLET FORMULATIONS . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/6073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bilal, Tessema. “PHYSICOCHEMICAL CHARACTERIZATION OF ACID MODIFIED DIOSCOREA STARCH AND ITS EVALUATION AS DIRECTLY COMPRESSIBLE EXCIPIENT AND DISINTEGRANT IN TABLET FORMULATIONS .” 2014. Thesis, Addis Ababa University. Accessed December 09, 2019. http://etd.aau.edu.et/dspace/handle/123456789/6073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bilal, Tessema. “PHYSICOCHEMICAL CHARACTERIZATION OF ACID MODIFIED DIOSCOREA STARCH AND ITS EVALUATION AS DIRECTLY COMPRESSIBLE EXCIPIENT AND DISINTEGRANT IN TABLET FORMULATIONS .” 2014. Web. 09 Dec 2019.

Vancouver:

Bilal T. PHYSICOCHEMICAL CHARACTERIZATION OF ACID MODIFIED DIOSCOREA STARCH AND ITS EVALUATION AS DIRECTLY COMPRESSIBLE EXCIPIENT AND DISINTEGRANT IN TABLET FORMULATIONS . [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2019 Dec 09]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bilal T. PHYSICOCHEMICAL CHARACTERIZATION OF ACID MODIFIED DIOSCOREA STARCH AND ITS EVALUATION AS DIRECTLY COMPRESSIBLE EXCIPIENT AND DISINTEGRANT IN TABLET FORMULATIONS . [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/6073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.