subject:(Cytokines)

1. Reuter, Simone. Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia.

Degree: Docteur es, Biologie Cellulaire et Nutrition, 2008, Université Henri Poincaré – Nancy I

URL: http://www.theses.fr/2008NAN10054

► La??-glutamyltransférase (GGT) est une enzyme membranaire qui catalyse l’hydrolyse du glutathion, le principal antioxydant cellulaire. Cette enzyme est surexprimée dans de nombreux cancers (p.ex. leucémies)… (more)

▼ La??-glutamyltransférase (GGT) est une enzyme membranaire qui catalyse l’hydrolyse du glutathion, le principal antioxydant cellulaire. Cette enzyme est surexprimée dans de nombreux cancers (p.ex. leucémies) et appartient à une famille multigénique d’au moins treize gènes. Le gène I, qui code pour la protéine active, est exprimé en trois ARNm (A, B et C), montrant tous la même phase de lecture ouverte mais différant dans leur région 5’ non traduite. A côté de l’implication de la GGT dans le développement des cancers, elle intervient également dans l’acquisition des résistances aux traitements anticancéreux et dans la synthèse des leukotriènes. Au cours de cette thèse, nous nous sommes intéressés aux mécanismes transcriptionnels qui régulent l’expression du gène I de la GGT. Plus particulièrement, nous avons étudié la régulation de la GGT après traitement des cellules K562 avec l’ester de phorbol, TPA, et la cytokine pro-inflammatoire, TNF?? Ces deux substances activent les facteurs de transcription AP-1 et NF-?B, qui sont connus pour réguler des gènes impliqués dans le développement des chimiorésistances. Nos résultats montrent que les deux promoteurs B et C de la GGT ainsi que les trois ARNm A, B et C, la protéine GGT ainsi que l’activité enzymatique de la GGT sont induits par le TNF?, mais pas par le TPA, dans la lignée cellulaire K562, qui est établie à partir de cellules issues d’une leucémie myéloïde chronique. Vu que l’induction du promoteur C de la GGT par le TNF? est fortement diminuée avec la curcumine, un agent chimiopréventif utilisé comme inhibiteur de la voie de signalisation NF-?B, nous avons émis une première hypothèse selon laquelle le facteur de transcription NF-?B serait impliqué dans la régulation du promoteur C de la GGT dans les cellules K562. Afin de prouver notre hypothèse nous avons utilisé des siRNA ciblant spécifiquement les deux sous-unités les plus représentées de NF-?B, p50 et p65, et effectivement l’induction du promoteur C de la GGT par le TNF? est inhibée par ces siRNA. Des expériences par co-transfection avec des protéines de la voie NF-?B, TRAF2, TRADD, I?B? et p65, confirment davantage cette hypothèse selon laquelle le promoteur C de la GGT est régulé par la voie de signalisation NF-?B dans les cellules K562. Des expériences par mutation ont ensuite permis d’identifier le site sur l’ADN qui est responsable de l’induction du promoteur C de la GGT par le TNF? dans les cellules K562. Ce site, localisé de -111 à -123 par rapport au site d’initiation de la transcription, est au moins capable de fixer le facteur de transcription p50 NF-?B. A proximité de ce site, nous avons identifié un autre site fonctionnel, localisé de - 73 à – 92 par rapport au site d’initiation de la transcription, qui lie le facteur de transcription Sp1. Des analyses par chromatine immunoprécipitation (ou ChIP) ont confirmé la liaison des facteurs de transcription NF-?B et Sp1 sur le promoteur C de la GGT et montrent de plus que l’ARN polymérase II est également recruté à cet endroit précis du promoteur. En outre,… Advisors/Committee Members: Diederich, Marc (thesis director).

Subjects/Keywords: Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reuter, S. (2008). Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia. (Doctoral Dissertation). Université Henri Poincaré – Nancy I. Retrieved from http://www.theses.fr/2008NAN10054

Chicago Manual of Style (16^th Edition):

Reuter, Simone. “Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia.” 2008. Doctoral Dissertation, Université Henri Poincaré – Nancy I. Accessed February 26, 2021. http://www.theses.fr/2008NAN10054.

MLA Handbook (7^th Edition):

Reuter, Simone. “Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia.” 2008. Web. 26 Feb 2021.

Vancouver:

Reuter S. Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia. [Internet] [Doctoral dissertation]. Université Henri Poincaré – Nancy I; 2008. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2008NAN10054.

Council of Science Editors:

Reuter S. Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia. [Doctoral Dissertation]. Université Henri Poincaré – Nancy I; 2008. Available from: http://www.theses.fr/2008NAN10054

University of Adelaide

2. Gameau, Louise J. (Louise Joy). The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau.

Degree: 1998, University of Adelaide

URL: http://hdl.handle.net/2440/19442

► The purpose of this project was to examine the cellular and molecular processes involved in expression of embryonic signals and the initial interactions that occur… (more)

Subjects/Keywords: Cytokines.

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APA (6^th Edition):

Gameau, L. J. (. J. (1998). The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gameau, Louise J (Louise Joy). “The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau.” 1998. Thesis, University of Adelaide. Accessed February 26, 2021. http://hdl.handle.net/2440/19442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gameau, Louise J (Louise Joy). “The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau.” 1998. Web. 26 Feb 2021.

Vancouver:

Gameau LJ(J. The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau. [Internet] [Thesis]. University of Adelaide; 1998. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2440/19442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gameau LJ(J. The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau. [Thesis]. University of Adelaide; 1998. Available from: http://hdl.handle.net/2440/19442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McGill University

3. Balasingam, Vijayabalan. Cytokines and Astroglial Reactivity.

Degree: PhD, Department of Neurology and Neurosurgery, 1995, McGill University

URL: https://escholarship.mcgill.ca/downloads/w6634599q.pdf ; https://escholarship.mcgill.ca/concern/theses/f1881p34k

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Astroglial reactivity is a prominent universal event following trauma and inflammation to the adult central nervous system. In contrast, injury inflicted during early postnatal life… (more)

Subjects/Keywords: Cytokines

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APA (6^th Edition):

Balasingam, V. (1995). Cytokines and Astroglial Reactivity. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/w6634599q.pdf ; https://escholarship.mcgill.ca/concern/theses/f1881p34k

Chicago Manual of Style (16^th Edition):

Balasingam, Vijayabalan. “Cytokines and Astroglial Reactivity.” 1995. Doctoral Dissertation, McGill University. Accessed February 26, 2021. https://escholarship.mcgill.ca/downloads/w6634599q.pdf ; https://escholarship.mcgill.ca/concern/theses/f1881p34k.

MLA Handbook (7^th Edition):

Balasingam, Vijayabalan. “Cytokines and Astroglial Reactivity.” 1995. Web. 26 Feb 2021.

Vancouver:

Balasingam V. Cytokines and Astroglial Reactivity. [Internet] [Doctoral dissertation]. McGill University; 1995. [cited 2021 Feb 26]. Available from: https://escholarship.mcgill.ca/downloads/w6634599q.pdf ; https://escholarship.mcgill.ca/concern/theses/f1881p34k.

Council of Science Editors:

Balasingam V. Cytokines and Astroglial Reactivity. [Doctoral Dissertation]. McGill University; 1995. Available from: https://escholarship.mcgill.ca/downloads/w6634599q.pdf ; https://escholarship.mcgill.ca/concern/theses/f1881p34k

4. Patrani, Maria. Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή.

Degree: 2016, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/38746

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Purpose: Although soluble urokinase plasminogen activator receptor (suPAR) is avalidated as a useful prognostic marker of sepsis outcome, its usefulness has neverbeen studied in patients… (more)

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Purpose: Although soluble urokinase plasminogen activator receptor (suPAR) is avalidated as a useful prognostic marker of sepsis outcome, its usefulness has neverbeen studied in patients with multiple traumas.Methods: Serum suPAR was measured in 85 patients with systemic inflammatoryresponse syndrome admitted in the Intensive Care Unit (ICU) due to multiple injurieswith injury severity index ≥15 without infection. Measurements were repeated afterfour days or at sepsis onset. The primary endpoint was the validity of suPAR toprognosticate development of multiple organ failure (MOF).Results: suPAR greater than or equal to 8ng/ml had 35.3% sensitivity, 84.5%specificity, 57.1% positive predictive value and 88.2% negative predictive value toindicate the development of MOF. This was confirmed by logistic regression analysis.Any over- time increase of suPAR more than or equal to 40% from the baselineadmission levels was associated with odds ratio 9.33 (p: 0.047) for the development of severe sepsis. The specificity of this change for the diagnosis of severe sepsiswas 90.0% and the positive predictive value 80.0%.Conclusions: suPAR is an accurate and independent biomarker for the prognosis ofthe development of MOF in critically ill non-septic patients admitted with multipletraumas in the ICU.Increase more than 40% from baseline admission levels isdiagnostic of the progression into severe sepsis

Σκοπός: Αν και o διαλυτός υποδοχέας του ενεργοποιητή του πλασμινογόνου τύπουουροκινάσης (suPAR) έχει επικυρωθεί ως χρήσιμος προγνωστικός δείκτης τηςέκβασης στη σήψη, η χρησιμότητά του δεν έχει ποτέ μελετηθεί σε ασθενείς μεπολλαπλούς τραυματισμούς.Μέθοδος: Ο suPAR μετρήθηκε στον ορό 85 ασθενών με σύνδρομο συστηματικήςφλεγμονώδους αντίδρασης που έκαναν εισαγωγή στη Μ.Ε.Θ. λόγω πολλαπλώντραυματισμών με δείκτη βαρύτητας τραύματος (ISS) ≥ 15 χωρίς παρουσία λοίμωξης.Οι μετρήσεις επαναλαμβάνονταν μετά από τέσσερις ημέρες ή κατά την έναρξη τηςσήψης. Ο βασικός στόχος ήταν η τεκμηρίωση της εγκυρότητας του suPAR στηνπρόγνωση εμφάνισης πολυοργανικής ανεπάρκειας.Αποτελέσματα: Τιμή suPAR ≥ 8ng/ml είχε 35,3% ευαισθησία, 84,5% ειδικότητα,57,1% θετική προγνωστική αξία και 88,2% αρνητική προγνωστική αξία να υποδείξειτην εμφάνιση πολυοργανικής ανεπάρκειας. Αυτό επιβεβαιώθηκε με ανάλυσηλογιστικής παλινδρόμησης. Με την πάροδο του χρόνου οποιαδήποτε αύξηση τουsuPAR ≥ 40% από τα επίπεδα αναφοράς κατά την εισαγωγή συνδυάστηκε με OR9,33 (p: 0,047) για την εμφάνιση σοβαρής σήψης. Η ειδικότητα αυτής της μεταβολήςγια τη διάγνωση σοβαρής σήψης ήταν 90% και η θετική προγνωστική αξία 80%.Συμπέρασμα: Ο suPAR είναι ένας ακριβής και ανεξάρτητος βιολογικός δείκτης γιατην πρόγνωση εμφάνισης πολυοργανικής ανεπάρκειας σε βαρέως πάσχοντες μησηπτικούς ασθενείς που έκαναν εισαγωγή στη Μ.Ε.Θ. με πολλαπλούςτραυματισμούς. Αύξηση > 40% από τα επίπεδα αναφοράς κατά την εισαγωγή είναι διαγνωστική για την εξέλιξη σε σοβαρή σήψη

Subjects/Keywords: Κυτταροκίνες; Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Patrani, M. (2016). Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/38746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Patrani, Maria. “Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή.” 2016. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed February 26, 2021. http://hdl.handle.net/10442/hedi/38746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Patrani, Maria. “Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή.” 2016. Web. 26 Feb 2021.

Vancouver:

Patrani M. Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10442/hedi/38746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Patrani M. Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. Available from: http://hdl.handle.net/10442/hedi/38746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oklahoma

5. Chollangi, Srinivas. STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M.

Degree: PhD, 2009, University of Oklahoma

URL: http://hdl.handle.net/11244/319327

► Oncostatin M (OSM) is a pleiotropic cytokine that belongs to the interleukin-6 (IL-6) family of cytokines. These cytokines play crucial role in diverse biological events… (more)

▼ Oncostatin M (OSM) is a pleiotropic cytokine that belongs to the interleukin-6 (IL-6) family of cytokines. These cytokines play crucial role in diverse biological events like inflammation, neuroprotection, hematopoiesis, metabolism and development. For example, when exposed to moderate levels of oxidative stress (i.e. bright cyclic light), IL-6 family cytokines are strongly upregulated in the retina. Using inhibition studies, we show that these upregulated cytokines play an essential role in protecting the neuronal cells from succumbing to subsequent lethal doses of oxidative stress. This wide range of functions elicited by IL-6 family cytokines are mediated by the formation of multimeric receptor complexes, which include a common receptor glycoprotein 130 (gp130). OSM is unique in this family since it can signal via two different receptor complexes i.e. LIFR:gp130 (type I) and OSMR:gp130 (type II). We have identified a unique helical loop on OSM between its B and C helices, which is not found on other IL-6 family cytokines. Based on its size and location, we hypothesized that the BC loop is responsible for OSM's unique ability to bind and activate OSMR. However, our experiments with mutated OSM molecules that have truncated BC loops showed that the BC loop does not play a role in OSM's unique ability to bind OSMR, but instead presents a steric hindrance for OSM's strong interaction with OSMR and LIFR. Kinetic and equilibrium binding analyses using surface plasmon resonance (SPR) and enzyme-linked immunosorbent assay (ELISA) support the evidence for improved binding and stability between mutant OSMs and the receptor complexes. Also, as a consequence of improved binding, these structurally modified OSMs exhibit enhanced ability in suppressing the proliferation of A375 melanoma cells and also protecting the neuronal cells in retina from oxidative stress, demonstrating that improved binding has functional consequences. Finally, we have evaluated the applicability of poly(ethylene glycol) (PEG) based hydrogels for delivering these molecules in a controlled manner. Using in vitro and in vivo studies in mice, we show that PEG hydrogels fabricated with PEG-diacrylate macromers with a molecular weight of 5000 Da can be used to deliver these cytokines in a sustained manner for ~12 days. And, for transscleral transport, a release rate of at least 0.5 ug/day is required for OSM to cross the scleral barriers and reach the retina to induce STAT3 activation. Advisors/Committee Members: Nollert, Mathias U||Ash, John D (advisor).

Subjects/Keywords: Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chollangi, S. (2009). STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/319327

Chicago Manual of Style (16^th Edition):

Chollangi, Srinivas. “STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M.” 2009. Doctoral Dissertation, University of Oklahoma. Accessed February 26, 2021. http://hdl.handle.net/11244/319327.

MLA Handbook (7^th Edition):

Chollangi, Srinivas. “STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M.” 2009. Web. 26 Feb 2021.

Vancouver:

Chollangi S. STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M. [Internet] [Doctoral dissertation]. University of Oklahoma; 2009. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/11244/319327.

Council of Science Editors:

Chollangi S. STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M. [Doctoral Dissertation]. University of Oklahoma; 2009. Available from: http://hdl.handle.net/11244/319327

University of Hong Kong

6. Wong, Hei-kiu. Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors.

Degree: 2009, University of Hong Kong

URL: http://hdl.handle.net/10722/55959

Subjects/Keywords: Cytokines.; Cytokines - Receptors.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, H. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/55959

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wong, Hei-kiu. “Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors.” 2009. Thesis, University of Hong Kong. Accessed February 26, 2021. http://hdl.handle.net/10722/55959.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wong, Hei-kiu. “Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors.” 2009. Web. 26 Feb 2021.

Vancouver:

Wong H. Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. [Internet] [Thesis]. University of Hong Kong; 2009. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10722/55959.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wong H. Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. [Thesis]. University of Hong Kong; 2009. Available from: http://hdl.handle.net/10722/55959

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

7. Wilkinson, Amanda. Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants.

Degree: PhD, Nutrition, 2015, Cornell University

URL: http://hdl.handle.net/1813/40599

► Maternal HIV infection during pregnancy is associated with increased risk of adverse obstetric and infant outcomes, and understanding the factors responsible for this association is… (more)

▼ Maternal HIV infection during pregnancy is associated with increased risk of adverse obstetric and infant outcomes, and understanding the factors responsible for this association is essential for designing effective interventions. Maternal malnutrition is an important determinant of maternal and infant health for both HIV-infected and - uninfected women. However, the relative contributions of food insecurity and infectioninduced changes in appetite and body weight regulation that alone, or in combination, contribute to malnutrition among HIV-infected women is largely unknown. The goal of this research was to advance the understanding of the underlying mechanisms by which maternal HIV infection influences pregnancy and early childhood outcomes. A prospective cohort of 114 pregnant women of mixed HIV-status was established in northwestern Tanzania. Pregnant women were followed through delivery and mother-infant dyads participated in follow-up visits at 1, 2, 3 and 6 months postpartum. This study had three main research aims: (1) to investigate maternal HIV and maternal anthropometric indicators as potential determinants of infant outcomes, (2) to characterize maternal and umbilical cord blood cytokine profiles and relate these to infant outcomes, and (3) to evaluate maternal cachexia characteristics during pregnancy. Maternal HIV status and maternal gestational mid-upper arm circumference (MUAC) were compared to infant anthropometric measurements from birth to six months of age. Infants born to HIV-infected mothers weighed, on average, 235 g less and were 1 cm shorter at birth compared to those born to HIV-uninfected mothers. Among mothers of mixed HIV-status, infants born to women with low MUAC had lower birth length and head circumference than infants born to women with higher MUAC. Maternal HIVinfection and low MUAC were also associated with poorer infant growth indicators through six months postpartum. Examination of cytokine profiles during pregnancy in this cohort revealed that HIV-infected pregnant women experienced elevated tumor necrosis factor (TNF)-[alpha]inflammation compared to HIV-uninfected pregnant women. On average, TNF-[alpha] concentrations were 14% higher among HIV-infected pregnant women. Among HIVinfected women, longer duration of antiretroviral therapy and more aggressive antiretroviral therapy were associated with more favorable cytokine profiles. Umbilical cord blood cytokine concentrations were used as a proxy for intrauterine exposure. Elevated umbilical cord blood cytokine concentrations were associated with lower infant birth weight and length. Pregnant women were also evaluated based on biopsychosocial characteristics related to cachexia. Based on a modified cachexia scoring system, HIV-infected and HIV-uninfected pregnant women had comparable cachexia symptoms. Evidence of increased gestational cachexia was associated with less favorable infant birth anthropometrics; and, among HIV-infected mothers, cachexia score was associated with lower infant weight-for-age and length-for-age… Advisors/Committee Members: McDermid,Joann M. (chair), Lujan,Marla E. (committee member), Booth,James (committee member), Brannon,Patsy Marie (committee member).

Subjects/Keywords: HIV; Pregnancy; Cytokines

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APA (6^th Edition):

Wilkinson, A. (2015). Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/40599

Chicago Manual of Style (16^th Edition):

Wilkinson, Amanda. “Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants.” 2015. Doctoral Dissertation, Cornell University. Accessed February 26, 2021. http://hdl.handle.net/1813/40599.

MLA Handbook (7^th Edition):

Wilkinson, Amanda. “Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants.” 2015. Web. 26 Feb 2021.

Vancouver:

Wilkinson A. Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1813/40599.

Council of Science Editors:

Wilkinson A. Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40599

Université Laval

8. Ribeiro de Vargas, Flavia. Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines.

Degree: 2016, Université Laval

URL: http://hdl.handle.net/20.500.11794/26686

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Le neutrophile est une cellule caractérisée par sa «plasticité» qui, dans des conditions pathologiques, lui permet d'acquérir des nouvelles fonctions qui sont impliquées dans la… (more)

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Le neutrophile est une cellule caractérisée par sa «plasticité» qui, dans des conditions pathologiques, lui permet d'acquérir des nouvelles fonctions qui sont impliquées dans la pathogenèse des maladies inflammatoires chroniques comme dans l’arthrite auto-immune (AAI). Les études suggèrent que le neutrophile peut être impliqué dans la résorption osseuse qui accompagne la maladie. Pour mieux analyser cette possibilité, nous avons mis des neutrophiles en présence d’un cocktail de cytokines présentes dans l’AAI afin d’analyser la modulation des gènes liés à la différenciation en cellule de type ostéoclaste. Les résultats ont démontré que le neutrophile est capable d’acquérir de nouvelles fonctions en présence de cytokines, notamment des fonctions importantes dans l’orchestration du système immunitaire et la réponse inflammatoire. Le neutrophile est également une source de molécules permettant la résolution de l’inflammation, notamment l’élafine, ce qui pourrait être un agent thérapeutique prometteur dans les maladies inflammatoires chroniques comme l’AAI.

The plasticity of neutrophils is a very useful property that in pathological conditions allow these cells to acquire new functions involved in the pathogenesis of chronic inflammatory diseases such as autoimmune arthritis (AIA). Studies suggest that neutrophils may be involved in bone resorption accompanying chronic inflammatory diseases. To better analyze this possibility, we have exposed neutrophils to a cocktail of cytokines present in AIA and studied their transcriptome to decipher the modulation of genes related to the differentiation into an osteoclast cell type. The results demonstrated the ability of neutrophils to acquire new functions when exposed to cytokines, including important functions involved in the orchestration of the immune system and inflammatory response. We also demonstrate that the neutrophil is a source of molecules involved in the resolution of inflammation, especially elafin, which seems to be a promising therapeutic agent in chronic inflammatory diseases and perhaps in AIA.

Advisors/Committee Members: Poubelle, Patrice.

Subjects/Keywords: Neutrophiles; Cytokines; Arthrite

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APA (6^th Edition):

Ribeiro de Vargas, F. (2016). Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/26686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ribeiro de Vargas, Flavia. “Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines.” 2016. Thesis, Université Laval. Accessed February 26, 2021. http://hdl.handle.net/20.500.11794/26686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ribeiro de Vargas, Flavia. “Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines.” 2016. Web. 26 Feb 2021.

Vancouver:

Ribeiro de Vargas F. Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines. [Internet] [Thesis]. Université Laval; 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/20.500.11794/26686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ribeiro de Vargas F. Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines. [Thesis]. Université Laval; 2016. Available from: http://hdl.handle.net/20.500.11794/26686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Deakin University

9. Mohd Noor, Suzita. Role of Jak2/Stat5/Cis pathway components in zebrafish development.

Degree: School of Medicine, 2011, Deakin University

URL: http://hdl.handle.net/10536/DRO/DU:30036712

► By utilizing the zebrafish as a research model, the function of specific cell signalling pathway components in development was investigated. This revealed new roles for… (more)

Subjects/Keywords: embryology; cytokines; zebrafish

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APA (6^th Edition):

Mohd Noor, S. (2011). Role of Jak2/Stat5/Cis pathway components in zebrafish development. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Thesis, Deakin University. Accessed February 26, 2021. http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Web. 26 Feb 2021.

Vancouver:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Internet] [Thesis]. Deakin University; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Thesis]. Deakin University; 2011. Available from: http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

10. Hedderson, William C. The Effects of Propranolol on Acute Inflammatory Cytokines and Muscle Function following an Exercise-Induced Muscle Injury to the Shoulder in Individuals with a High Pain Sensitivity COMT Polymorphism.

Degree: PhD, Health and Human Performance - Applied Physiology and Kinesiology, 2019, University of Florida

URL: https://ufdc.ufl.edu/UFE0051842

► The current pharmacological treatment options for pain are generally unsuccessful and not recommended for long term use. Treatment of chronic pain is also a huge… (more)

▼ The current pharmacological treatment options for pain are generally unsuccessful and not recommended for long term use. Treatment of chronic pain is also a huge economic burden on society. Current pharmacological interventions for pain include the use of opioids and nonsteroidal anti inflammatory medication, these treatments either have a low success rate or put the patient at an increased risk for complications following long term use. Exploring alternative pharmacological interventions to treat pain in an improve care and outcome measurements for pain. One avenue of exploration is the use of personalized medicine. Previous studies have identified the COMT gene in conjunction with pain catastrophizing to be able to predict pain intensity in the shoulder after an exercise induced muscle injury. Current literature suggests that the non specific beta blocker, propranolol, may effectively mediate pain in these individuals but there is much to understand how propranolol can mediate pain, and whether propranolol works as an anti inflammatory or as an opiate. This study identified if propranolol has any anti inflammatory properties in blood plasma following an exercise induced muscle injury. 54 participants that were positive for the COMT genotype and pain catastrophizing were enrolled in the study. Participants were randomly assigned to the propranolol or the placebo group. The protocol required participants to complete baseline testing, which included a blood draw and then perform a fatigue protocol intended to cause an exercise induced muscle injury to the shoulder. Participants returned at 24 hour intervals for follow up measurements. Levels of inflammatory cytokines were measured in the blood plasma, these include TNFalpha, IL1beta, IL6, IL8, IL10 and CRP. There were no between group differences observed, indicating that propranolol does not alter blood plasma levels of inflammatory cytokines after an acute exercise induced muscle injury. ( en ) Advisors/Committee Members: Borsa,Paul A (committee chair), Coombes,Stephen A (committee member), Fillingim,Roger Benton (committee member), George,Steven (committee member).

Subjects/Keywords: cytokines – inflammation – propranolol

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APA (6^th Edition):

Hedderson, W. C. (2019). The Effects of Propranolol on Acute Inflammatory Cytokines and Muscle Function following an Exercise-Induced Muscle Injury to the Shoulder in Individuals with a High Pain Sensitivity COMT Polymorphism. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0051842

Chicago Manual of Style (16^th Edition):

Hedderson, William C. “The Effects of Propranolol on Acute Inflammatory Cytokines and Muscle Function following an Exercise-Induced Muscle Injury to the Shoulder in Individuals with a High Pain Sensitivity COMT Polymorphism.” 2019. Doctoral Dissertation, University of Florida. Accessed February 26, 2021. https://ufdc.ufl.edu/UFE0051842.

MLA Handbook (7^th Edition):

Hedderson, William C. “The Effects of Propranolol on Acute Inflammatory Cytokines and Muscle Function following an Exercise-Induced Muscle Injury to the Shoulder in Individuals with a High Pain Sensitivity COMT Polymorphism.” 2019. Web. 26 Feb 2021.

Vancouver:

Hedderson WC. The Effects of Propranolol on Acute Inflammatory Cytokines and Muscle Function following an Exercise-Induced Muscle Injury to the Shoulder in Individuals with a High Pain Sensitivity COMT Polymorphism. [Internet] [Doctoral dissertation]. University of Florida; 2019. [cited 2021 Feb 26]. Available from: https://ufdc.ufl.edu/UFE0051842.

Council of Science Editors:

Hedderson WC. The Effects of Propranolol on Acute Inflammatory Cytokines and Muscle Function following an Exercise-Induced Muscle Injury to the Shoulder in Individuals with a High Pain Sensitivity COMT Polymorphism. [Doctoral Dissertation]. University of Florida; 2019. Available from: https://ufdc.ufl.edu/UFE0051842

University of Waterloo

11. Nagaarudkumaran, Nijani. In Vitro Release of Inflammatory Cytokines from Contact Lens Materials.

Degree: 2020, University of Waterloo

URL: http://hdl.handle.net/10012/16595

► The upregulation of inflammatory mediators in the tear film during contact lens wear indicates that lens wear may induce an inflammatory response on the ocular… (more)

Subjects/Keywords: contact lens; cytokines

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APA (6^th Edition):

Nagaarudkumaran, N. (2020). In Vitro Release of Inflammatory Cytokines from Contact Lens Materials. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/16595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nagaarudkumaran, Nijani. “In Vitro Release of Inflammatory Cytokines from Contact Lens Materials.” 2020. Thesis, University of Waterloo. Accessed February 26, 2021. http://hdl.handle.net/10012/16595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nagaarudkumaran, Nijani. “In Vitro Release of Inflammatory Cytokines from Contact Lens Materials.” 2020. Web. 26 Feb 2021.

Vancouver:

Nagaarudkumaran N. In Vitro Release of Inflammatory Cytokines from Contact Lens Materials. [Internet] [Thesis]. University of Waterloo; 2020. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10012/16595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nagaarudkumaran N. In Vitro Release of Inflammatory Cytokines from Contact Lens Materials. [Thesis]. University of Waterloo; 2020. Available from: http://hdl.handle.net/10012/16595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

12. Vorage, M.S.C.E. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.

Degree: 2011, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/213023

► Fibromyalgia (FM) is a common disorder of unclear aetiology, characterized by chronic widespread pain and painful tender points (body pressure points). Several researchers suggest that… (more)

Subjects/Keywords: Fibromyalgia; cytokines; oxidative stress

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APA (6^th Edition):

Vorage, M. S. C. E. (2011). What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/213023

Chicago Manual of Style (16^th Edition):

Vorage, M S C E. “What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.” 2011. Masters Thesis, Universiteit Utrecht. Accessed February 26, 2021. http://dspace.library.uu.nl:8080/handle/1874/213023.

MLA Handbook (7^th Edition):

Vorage, M S C E. “What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.” 2011. Web. 26 Feb 2021.

Vancouver:

Vorage MSCE. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. [Internet] [Masters thesis]. Universiteit Utrecht; 2011. [cited 2021 Feb 26]. Available from: http://dspace.library.uu.nl:8080/handle/1874/213023.

Council of Science Editors:

Vorage MSCE. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. [Masters Thesis]. Universiteit Utrecht; 2011. Available from: http://dspace.library.uu.nl:8080/handle/1874/213023

13. Sonia Devi Lourembam. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.

Degree: Biotechnology, 2011, Tezpur University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/9014

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Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread… (more)

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Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread in south east Asia with 10 of the 11 countries endemic for malaria and with 1.2 billion people at risk of exposure to the disease. India reported the highest malaria confirmed (1563344) and death (1133) cases in 2009 from this region (WHO). The north-eastern region of India (population 28.5 million) is highly endemic for malaria and has been declared as a high risk zone by National Anti Malaria Program. Assam contributes to 64.7% of the malaria positive cases in the north-eastern region of which P. falciparum accounts for 58-75% of the cases and the remainder are due to Plasmodium vivax. Though people living in endemic area develop immunity, which can be either anti disease or anti infection immunity, the factors delineating them are not clear. It is thus felt that an in depth understanding of the immune response leading to anti infection or to anti disease responses in population genetic studies and elucidation of parasite diversity is required for success of malaria control programs. The present study was designed against this perspective with the following objectives: 1) To study the clone multiplicity of the parasite genotypes existing in the study area 2) To elucidate the protective humoral immune response. 3) To elucidate the protective cell mediated immune response. The study was conducted at Guabari village of Baksa district and Kondoli Tea Estate (KTE) of Nagaon district which are mesoendemic for malaria. The two sites differ in population demography and accessibility to health care. Plasmodium falciparum diversity was assessed by typing polymorphic block 2 region of Merozoite Surface Protein 1(MSP-1) gene using primary as well as nested PCR cycles. A high degree of polymorphism in isolates from the two study sites was seen with 33 alleles of MSP-1 seen in Guabari village and 18 alleles at KTE.

References p.143-164, Abstract given chapter wise

Advisors/Committee Members: Baruah, S.

Subjects/Keywords: Biology; Plasmodium falciparum; Malaria; Cytokines

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APA (6^th Edition):

Lourembam, S. D. (2011). Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. (Thesis). Tezpur University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/9014

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.” 2011. Thesis, Tezpur University. Accessed February 26, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/9014.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.” 2011. Web. 26 Feb 2021.

Vancouver:

Lourembam SD. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. [Internet] [Thesis]. Tezpur University; 2011. [cited 2021 Feb 26]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lourembam SD. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. [Thesis]. Tezpur University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

14. Steyn, Paul. Cytokine super-families affect adult stem cells : IL-6 and the skeletal muscle niche.

Degree: Physiological Sciences, 2011, Stellenbosch University

URL: http://hdl.handle.net/10019.1/6550

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Thesis (MSc (Physiological Sciences)) – University of Stellenbosch, 2011.

ENGLISH ABSTRACT: Background: IL-6 belongs to a cytokine super-family known to affect cell proliferation, although other family… (more)

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Thesis (MSc (Physiological Sciences)) – University of Stellenbosch, 2011.

ENGLISH ABSTRACT: Background: IL-6 belongs to a cytokine super-family known to affect cell proliferation, although other family members are better characterized. Proliferation promoting factors (IL-6) compete with differentiation promoting factors (myogenic regulatory factors: MyoD and myogenin) to affect cell cycle. Cell cycle progression is assessed by determining the proportion of cells shifting from arrest to chromatin synthesis and mitosis phases (G0/G1 and S and G2/M respectively). Methods: This study assessed the effects of IL-6 on cell cycle progression and proliferation vs. differentiation of C2C12 skeletal myoblasts. Physiological doses (10 or 100 pg/ml) were compared to a high dose (10 ng/ml), with exposure lasting 48 hours (addition of IL-6 dose to proliferation medium at 0 and 24 hours). Acute signaling downstream of the IL-6 gp130 receptor was assessed after the first exposure. Results: Propidium iodide analysis of nuclear material using flow cytometry indicated shifts in forward scatter. Both Low and Medium doses shifted a greater proportion (p<0.05) of cells from G0/G1 to S and G2M phases at 24 hours and all doses resulted in the same shift (p<0.05) at the 48 hour time point. However, the High dose significantly (p<0.05) increased myogenin expression at the 48 hour time point. Microscopy indicated that confluence was prevented by low seeding density and did not influence the result. Cells harvested at 5 minutes post stimulation indicated that all doses significantly increased STAT3 phosphorylation. 10 minutes post stimulation the High dose group sustained elevated levels of STAT3 phosphorylation. Conclusions: Low and medium doses of IL-6 increase proliferation in a muscle satellite cell line by activating cell division and allowing myoblasts to remain in the active cell cycle. High doses of IL-6 increase differentiation by mediating upregulation of myogenic regulatory factors and this is thought to be due to prolonged STAT3 activation. Physiological control of myoblast behaviour by cytokines is evident and such control would be influenced by the severity of the endogenous cytokine response to various stimuli.

AFRIKAANSE OPSOMMING: Agtergrond: IL-6 behoort aan n sitokien super-familie bekend vir die affektering van sel verspreiding, alhoewel ander familie lede beter gekenmerk is. Bevordering van verspreiding faktore (IL-6) kompeteer met bevordering van differensiasie fatore (myogenic regulatory factors: MyoD en myogenin) om die sel siklus te affekteer. Sel siklus progressie word geassesseer deur die bepaling van die proporsie selle wat verskuif van arrestasie na chromatien sintese en mitose fases (G0/G1 en S en G2/M onderskeidelik). Metodes: Hierdie studie het die effekte van IL-6 op die progressie van die sel siklus geassesseer asook die proliferasie vs. differensiasie van C2C12 skelet spier satelliet selle. Fisiologiese dosisse (10 en 100 pg/ml) was vergelyk tot n hoog dose (10…

Advisors/Committee Members: Myburgh, Kathryn H., Smith, Robert M., University of Stellenbosch. Faculty of Science. Dept. of Physiological Sciences..

Subjects/Keywords: Physiological sciences; Stem cells; Cytokines

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APA (6^th Edition):

Steyn, P. (2011). Cytokine super-families affect adult stem cells : IL-6 and the skeletal muscle niche. (Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/6550

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Steyn, Paul. “Cytokine super-families affect adult stem cells : IL-6 and the skeletal muscle niche.” 2011. Thesis, Stellenbosch University. Accessed February 26, 2021. http://hdl.handle.net/10019.1/6550.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Steyn, Paul. “Cytokine super-families affect adult stem cells : IL-6 and the skeletal muscle niche.” 2011. Web. 26 Feb 2021.

Vancouver:

Steyn P. Cytokine super-families affect adult stem cells : IL-6 and the skeletal muscle niche. [Internet] [Thesis]. Stellenbosch University; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10019.1/6550.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Steyn P. Cytokine super-families affect adult stem cells : IL-6 and the skeletal muscle niche. [Thesis]. Stellenbosch University; 2011. Available from: http://hdl.handle.net/10019.1/6550

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Nelson Mandela Metropolitan University

15. [No author]. The effects of terpenoids on the expression and function of cytokines and adipokines in pre-adipocytes and differentiated adipocytes.

Degree: Faculty of Science, 2017, Nelson Mandela Metropolitan University

URL: http://hdl.handle.net/10948/9208

► CURRENTLY UNDER EMBARGO UNTIL THE 26/4/2019: Type 2 diabetes is a metabolic disorder characterised by inflammation, insulin resistance and the inability of pancreatic β-cells to… (more)

▼ CURRENTLY UNDER EMBARGO UNTIL THE 26/4/2019: Type 2 diabetes is a metabolic disorder characterised by inflammation, insulin resistance and the inability of pancreatic β-cells to secrete enough insulin to produce a physiological effect. Obesity and high levels of triacylglycerol’s are associated with the development of Type 2 diabetes. Adipose tissue is an active endocrine organ that secretes various protein and peptide hormones, known as adipokines, which mediate important metabolic functions. In an insulin resistant and hyperglycaemic state, levels of anti-inflammatory adipokines, adiponectin, are reduced, whereas levels of pro-inflammatory cytokines, interleukin-6 and interleukin-1β, are elevated; this results in a shift from an anti- to a pro-inflammatory state that is accompanied by dysfunction and apoptosis of the pancreatic β-cells. Cannabis sativa L. has been traditionally used as an anti-inflammatory agent in Southern Africa, specifically treating snakebites, fever and malaria. Δ9-tetrahydrocannabinol is the main psychoactive compound derived from C. sativa, whereas the other major cannabinoids, cannabinol and cannabidiol, have shown anti-inflammatory and sedative properties respectively. Marrubiin is a compound derived from the plant Leonotis leonurus L. and has been traditionally used as an anti-inflammatory and anti-diabetic agent. To determine the effects of these compounds in a hyperglycaemic state, pre- and differentiated mouse adipocytes (3T3-L1 cells) were exposed for seven and fourteen days to the following treatments: Δ9-tetrahydrocannabinol, cannabidiol, cannabinol, marrubiin, anandamide (an endogenous endocannabinoid) and cannabis extract, individually and in combination, under normal glucose and hyperglycaemic conditions. Levels of adiponectin, interleukin-6, leptin, tumour-necrosis factor-α and interleukin-1β were quantified using mouse enzyme-linked immunosorbent assay kits and Oil Red O staining was carried out to determine lipid distribution and lipid droplet characteristics. Results indicate that various cannabinoids, in combination, mediate an anti-inflammatory effect by decreasing the expression of various pro-inflammatory cytokines, which may have allowed for a shift from a pro- to an anti-inflammatory state by these compounds, and may also contribute to the reduction of lipid, which may be used as a supplementary option to current diabetic treatment regimes.

Subjects/Keywords: Terpenes; Cytokines; Fat cells

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APA (6^th Edition):

author], [. (2017). The effects of terpenoids on the expression and function of cytokines and adipokines in pre-adipocytes and differentiated adipocytes. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/9208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “The effects of terpenoids on the expression and function of cytokines and adipokines in pre-adipocytes and differentiated adipocytes.” 2017. Thesis, Nelson Mandela Metropolitan University. Accessed February 26, 2021. http://hdl.handle.net/10948/9208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “The effects of terpenoids on the expression and function of cytokines and adipokines in pre-adipocytes and differentiated adipocytes.” 2017. Web. 26 Feb 2021.

Vancouver:

author] [. The effects of terpenoids on the expression and function of cytokines and adipokines in pre-adipocytes and differentiated adipocytes. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2017. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10948/9208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. The effects of terpenoids on the expression and function of cytokines and adipokines in pre-adipocytes and differentiated adipocytes. [Thesis]. Nelson Mandela Metropolitan University; 2017. Available from: http://hdl.handle.net/10948/9208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Μπούμπαλη, Σταυρούλα. Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα.

Degree: 2009, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3419

► Το ασβέστιο αποτελεί ένα σημαντικό δεύτερο μήνυμα που ρυθμίζει την ανοσολογική απόκριση. Η αύξηση του ενδοκυττάριου ασβεστίου αυξάνει την παραγωγή της IL-10 στα Τ-λεμφοκύτταρα, αλλά… (more)

▼ Το ασβέστιο αποτελεί ένα σημαντικό δεύτερο μήνυμα που ρυθμίζει την ανοσολογική απόκριση. Η αύξηση του ενδοκυττάριου ασβεστίου αυξάνει την παραγωγή της IL-10 στα Τ-λεμφοκύτταρα, αλλά δεν είναι σαφές ποια ενδοκυττάρια μόρια εμπλέκονται σε αυτή τη ρύθμιση. Σκοπός της μελέτης είναι η αποσαφήνιση της συμμετοχής του ασβεστίου στην παραγωγή της IL-10 από Τ-λεμφοκύτταρα και η εντόπιση των πιθανών ενδοκυττάριων στόχων. Ο ρόλος των ενζύμων κλειδιών στην οδό ενεργοποίησης μέσω ασβεστίου καλσινευρίνης και CaM Kinase II μελετήθηκε με χρήση είτε ειδικών αναστολέων, κυκλοσπορίνης και ΚΝ-62 αντίστοιχα, είτε με πλασμίδια που κωδικοποιούν τις καταλυτικές υπομονάδες των ενζύμων καλσινευρίνη και CaM Kinase II. Ανθρώπινα Τ-λεμφοκύτταρα περιφερικού αίματος διεγέρθηκαν με anti-CD3/anti-CD28 (διέγερση μέσω TCR), ή Ionomycin/PMA (ενδοκυττάρια στόχευση Ca++ και PKC) παρουσία ή απουσία των ειδικών αναστολέων. Το πρωτεϊνικό προϊόν της IL-10 μετρήθηκε με ELISA ενώ η παραγωγή mRNA της IL-10 με Real Time PCR. Η ενεργότητα του υποκινητή της IL-10, IL-2, IL-4 παρουσία ή απουσία των ενεργών ενζύμων ελέγχθηκε με διαμόλυνση των κυττάρων με πλασμίδια που φέρουν τον υποκινητή του γονιδίου (1327 bp) ή τμήματα αυτού (-1010, -500, -310, -235, -135 bp Η δράση των ίδιων ενζύμων στην ενεργότητα των μεταγραφικών παραγόντων MEF2, CREB και NF-κB ελέγχθηκε με ταυτόχρονη έκφρασή τους in vivo με πειράματα διαμολύνσεως με πλασμίδια που ελέγχουν την ενεργότητα της λουσιφεράσης υπό τον έλεγχο τους και in vitro σε πυρηνικά πρωτεϊνικά εκχυλίσματα με πειράματα EMSA. Η επίδραση του μεταγραφικού παράγοντα MEF2 στον υποκινητή της IL-10 έγινε μέσω υπερέκφρασης του in vivo σε πειράματα διαμόλυνσης. Η IL-10 ανιχνεύεται 24 ώρες μετά τη διέγερση των κυττάρων, φτάνει στο μέγιστο στις 48 ώρες και μειώνεται μετά τις 72 ώρες. Παρουσία κυκλοσπορίνης η παραγωγή της IL-10 μειώθηκε κατά 80%-100%, ενώ παρουσία ΚΝ-62 η παραγωγή της IL-10 μειώθηκε κατά 70%-90%, σε όλα τα χρονικά διαστήματα στα οποία μελετήθηκε. Τα ποσοστά αναστoλής ήταν ίδια και κατά τους δύο τρόπους διέγερσης. Οταν τα κύτταρα διεγέρθηκαν μέσω TCR και του συνδιεγερτικού μορίου CD28, η αναστολή των παραπάνω ενζύμων δεν επηρεάζει την συσσώρευση του mRNA της IL-10, υποδηλώνοντας ότι η ρύθμιση γίνεται σε μεταγενέστερα της μεταγραφής στάδια, επηρεάζοντας πιθανά τη σταθερότητα του mRNA ή το ρυθμό μετάφρασής του. Όταν ο τρόπος διέγερσης των κυττάρων παρέκαμπτε τον TCR τότε η ελάττωση της παραγωγής της IL-10 αντανακλούσε και σε επίπεδο mRNA. Η ενεργοποίηση του υποκινητή της IL-10 που παρατηρείται μετά από διέγερση με ΙON/PMA και CD3/CD28 αυξήθηκε κατά 5 και 3 φορές αντίστοιχα σε Τ λεμφοκύτταρα τα οποία είχαν διαμολυνθεί με τις ενεργές μορφές των ενζύμων καλσινευρίνη και CaM Kinase II σε σχέση με τα κύτταρα τα οποία είχαν διαμολυνθεί μόνο με το όχημα pSRa. Η δράση της CaM Kinase II είναι εκλεκτική για τον υποκινητή της ΙL-10 εφόσον το ίδιο ένζυμο μειώνει την δραστικότητα του υποκινητή τόσο μιας άλλης Th2 κυτταροκίνης όπως η IL-4 όπως επίσης και της IL-2, ενώ η καλσινευρίνη έχει θετική επίδραση στους υποκινητές και των… Advisors/Committee Members: Παληογιάννη, Φωτεινή, Boumbali, Stavroula, Δημητρακόπουλος, Γεώργιος, Παπαβασιλείου, Αθανάσιος, Αναστασίου, Ευάγγελος, Χρυστοφίδου, Μυρτώ, Ζαρκάδης, Ιωάννης, Κολονίτσου, Φεβρονία.

Subjects/Keywords: Ανοσολογία; Κυτταροκίνες; 571.966; Immunology; Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Μπούμπαλη, �. (2009). Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα. (Doctoral Dissertation). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3419

Chicago Manual of Style (16^th Edition):

Μπούμπαλη, Σταυρούλα. “Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα.” 2009. Doctoral Dissertation, University of Patras. Accessed February 26, 2021. http://nemertes.lis.upatras.gr/jspui/handle/10889/3419.

MLA Handbook (7^th Edition):

Μπούμπαλη, Σταυρούλα. “Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα.” 2009. Web. 26 Feb 2021.

Vancouver:

Μπούμπαλη �. Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα. [Internet] [Doctoral dissertation]. University of Patras; 2009. [cited 2021 Feb 26]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3419.

Council of Science Editors:

Μπούμπαλη �. Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα. [Doctoral Dissertation]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3419

University of Manchester

17. Schiro, Andrew. The use of microparticles and inflammatory cytokines as potential biomarkers for plaque instability in patients with carotid disease.

Degree: 2015, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:280242

► AbstractAim: Endothelial microparticles (EMPs) are released from dysfunctional endothe-lial cells. We hypothesised that patients with unstable carotid plaque have higher levels of circulating microparticles compared… (more)

▼ AbstractAim: Endothelial microparticles (EMPs) are released from dysfunctional endothe-lial cells. We hypothesised that patients with unstable carotid plaque have higher levels of circulating microparticles compared to patients with stable plaques, and this may correlate with serum markers of plaque instability and inflammation.Method: Circulating EMPs and inflammatory markers were measured in twenty healthy controls and seventy patients undergoing carotid endarterectomy. EMPs were quantified using flow cytometry. Bioplex assays profiled systemic inflamma-tory and bone-related proteins. Immunohistological analysis detailed the contribu-tion of differentially-regulated systemic markers to plaque pathology. Alizarin red staining showed calcification. Results: EMPs were significantly higher in patients with carotid stenosis (≥70%) compared to controls, with no differences between asymptomatic vs symptomatic patients. Asymptomatic patients with unstable plaques exhibited higher levels of EMPs compared to those with stable plaques, with a similar trend observed in symptomatic patients. CXCL9 and SCGF-β were significantly elevated in asymptomatic patients with unstable plaques, with IL-16 and macrophage inhibitory factor significantly elevated in the stable plaque group. CXCL9, CTACK and SCGF-β were detected within all plaques, suggesting a contribution to both localised and systemic inflammation. Osteopontin and osteoprotegerin were significantly elevated in the symptomatic vs asymptomatic group, while osteocalcin was higher in asymptomatic patients with stable plaque. All plaques exhibited calcification, which was significantly greater in asymptomatic patients. This may impact on plaque stability. Conclusions: Circulatory EMP, CXCL9 and SCGF-β levels are raised in asymp-tomatic patients with unstable plaques, which could be important in identifying patients at most benefit from intervention. Advisors/Committee Members: ALEXANDER, YVONNE Y, SERRACINO-INGLOTT, FERDINAND FF, Alexander, Yvonne, Serracino-Inglott, Ferdinand, Boulton, Andrew.

Subjects/Keywords: Stroke; endothelial microparticles; cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schiro, A. (2015). The use of microparticles and inflammatory cytokines as potential biomarkers for plaque instability in patients with carotid disease. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:280242

Chicago Manual of Style (16^th Edition):

Schiro, Andrew. “The use of microparticles and inflammatory cytokines as potential biomarkers for plaque instability in patients with carotid disease.” 2015. Doctoral Dissertation, University of Manchester. Accessed February 26, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:280242.

MLA Handbook (7^th Edition):

Schiro, Andrew. “The use of microparticles and inflammatory cytokines as potential biomarkers for plaque instability in patients with carotid disease.” 2015. Web. 26 Feb 2021.

Vancouver:

Schiro A. The use of microparticles and inflammatory cytokines as potential biomarkers for plaque instability in patients with carotid disease. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Feb 26]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:280242.

Council of Science Editors:

Schiro A. The use of microparticles and inflammatory cytokines as potential biomarkers for plaque instability in patients with carotid disease. [Doctoral Dissertation]. University of Manchester; 2015. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:280242

University of Adelaide

18. Hiwase, Devendra. Evaluation of anti-proliferative and pro-apoptotic effects of tyrosine kinase inhibitors on CML-CD34+ cells.

Degree: 2010, University of Adelaide

URL: http://hdl.handle.net/2440/65253

► Although imatinib (IM) has revolutionalised CML management, 30 to 40% patients fail IM therapy. Many of these patients can be rescued with second generation tyrosine… (more)

▼ Although imatinib (IM) has revolutionalised CML management, 30 to 40% patients fail IM therapy. Many of these patients can be rescued with second generation tyrosine kinase inhibitors (TKI), dasatinib, nilotinib and bosutinib. This research elucidates the dasatinib cellular transport pathways and its role in mediating dasatinib resistance. It also assesses dynamics of Bcr-Abl kinase inhibition and apoptosis in CML lines and CML-CD34+ progenitors. Lastly it addresses the role of cytokines in mediating TKI resistance and possible combination therapy to circumvent cytokine mediated TKI resistance. The organic cation transporter (OCT-1) mediates IM influx and low OCT-1 activity is a major contributor to suboptimal response in CML patients treated with IM. In the current study the relevance of OCT-1 activity and efflux pumps in determining intracellular concentration (IUR) of dasatinib were assessed. In contrast to IM, dasatinib cellular uptake is not significantly affected by OCT-1 activity, so that expression and function of OCT-1 is unlikely to affect response to dasatinib. Dasatinib is a substrate of efflux proteins, ABCB1 and ABCG2. Overexpression of these proteins can mediate dasatinib resistance. There is increasing evidence that nilotinib is an ABCB1 inhibitor. These different interactions of dasatinib and nilotinib with ABCB1 were exploited for combination therapy. Nilotinib increased 14CDasatinib IUR and had synergistic effect in inducing cell death in ABCB1 overexpressing cells. These data suggest that combinations of these two TKI can overcome ABCB1 mediated dasatinib resistance and may allow the use of lower concentrations of each drug. In contrast to IM, dasatinib cellular influx is predominantly passive and maximum intracellular concentration is achieved within a few minutes. This was further confirmed by the observation of maximum Bcr-Abl kinase inhibition within 30 minutes of culture with dasatinib. Despite reactivation of Bcr-Abl kinase within 30 minutes of drug washout, short-term (30 minutes) intense (>90%) Bcr-Abl kinase inhibition with dasatinib triggers apoptosis in CML cell lines. This is in contrast to the previously established paradigm that continuous kinase inhibition is required for optimal response to IM. These results were further supported by a recently published dasatinib dose optimisation study. Further work in this thesis demonstrated that although Bcr-Abl kinase reactivates within 30 minutes of drug washout, the prosurvival proteins Erk, AKT and STAT5 dephosphorylated rapidly while the apoptotic proteins remained phosphorylated. This differential degradation of prosurvival and apoptotic proteins might be responsible for a state of “oncogenic-shock”, as described by Sharma et al. Subsequent studies demonstrated that in the absence of cytokines, short-term intense Bcr-Abl kinase inhibition with therapeutically achievable concentration of dasatinib (100 nM dasatinib) eliminated 70 to 80% of CML-CD34+ progenitors. However, in the presence of cytokines despite >90% Bcr-Abl kinase … Advisors/Committee Members: Hughes, Timothy Peter (advisor), Kumar, Sharad (advisor), Vaz de Melo, Junia (advisor), White, Deborah (advisor), School of Medicine (school).

Subjects/Keywords: chronic myeloid leukemia; dasatinib; cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hiwase, D. (2010). Evaluation of anti-proliferative and pro-apoptotic effects of tyrosine kinase inhibitors on CML-CD34+ cells. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/65253

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hiwase, Devendra. “Evaluation of anti-proliferative and pro-apoptotic effects of tyrosine kinase inhibitors on CML-CD34+ cells.” 2010. Thesis, University of Adelaide. Accessed February 26, 2021. http://hdl.handle.net/2440/65253.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hiwase, Devendra. “Evaluation of anti-proliferative and pro-apoptotic effects of tyrosine kinase inhibitors on CML-CD34+ cells.” 2010. Web. 26 Feb 2021.

Vancouver:

Hiwase D. Evaluation of anti-proliferative and pro-apoptotic effects of tyrosine kinase inhibitors on CML-CD34+ cells. [Internet] [Thesis]. University of Adelaide; 2010. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2440/65253.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hiwase D. Evaluation of anti-proliferative and pro-apoptotic effects of tyrosine kinase inhibitors on CML-CD34+ cells. [Thesis]. University of Adelaide; 2010. Available from: http://hdl.handle.net/2440/65253

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

19. Chin, Peck Yin. Cytokines and programming the pre-implantation embryo.

Degree: 2014, University of Adelaide

URL: http://hdl.handle.net/2440/92044

► As the pre-implantation embryo traverses the female reproductive tract, it experiences fluctuations in the composition of the surrounding maternal environment, including the availability of nutrients,… (more)

▼ As the pre-implantation embryo traverses the female reproductive tract, it experiences fluctuations in the composition of the surrounding maternal environment, including the availability of nutrients, growth factors and cytokines. In particular, the cytokine milieu surrounding the early embryo is pivotal in programming optimal embryo development. The pre-implantation embryo is sensitive to a range of perturbations such as maternal diet or in vitro culture. These and other insults influencing the maternal environment including infection, stress and environmental toxins may in part act via impact on oviduct and uterine cytokine synthesis. However the effect of maternal perturbation to inflammation or infection, on the embryo and the role of cytokines in mediating this is not fully elucidated. The studies described in this thesis employed an in vivo mouse model of maternal systemic inflammation with the proinflammatory bacterial lipopolysaccharide (LPS), where a pro-inflammatory cytokine response was elicited on days 2.5 and 3.5 post coitum (pc), prior to implantation. This model was studied in wildtype C57Bl/6 (Il10 ⁺ʹ⁺) mice and mice with a null mutation in the Il10 gene (Il10 ⁻ʹ⁻) were studied to investigate the effects of maternal deficiency in the anti-inflammatory cytokine IL-10 during LPS treatment. We demonstrated that the altered cytokine signals resulting from a low level pro inflammatory LPS challenge (0.5 μg/mouse) in the pre-implantation period elicit changes in the embryo developmental trajectory that in turn alter fetal growth and delay postnatal growth in the male progeny from LPS-treated mothers. As LPS did not directly impact development of the embryo at low and moderate doses, this result appears to reflect indirect effects of LPS mediated via the maternal tract. This is consistent with data from day 3.5 pc oviduct and uterus tissues which revealed increased mRNA expression of proinflammatory cytokines including Il6, Tnfa and Il12b following maternal LPS treatment. Peri-conceptional low dose LPS treatment in Il10 ⁺ʹ⁺ and Il10 ⁻ʹ⁻ mice revealed that the number of viable fetuses and fetal weight were both significantly reduced after LPS treatment, particularly in the Il10 ⁻ʹ⁻ mice. Embryo transfer was then utilised to investigate the mechanism by which LPS acts on the embryo, where day 3.5 pc embryos from donors treated with 0.5 μg LPS or PBS on days 2.5 and 3.5pc were transferred into day 2.5 pc pseudopregnant Swiss female recipients. The effect of maternal LPS treatment on fetal and placental development was seen to be maintained even after embryo transfer, suggesting that any effects of altered cytokine expression in embryos are exerted during cleavage stages before embryo recovery from donors. In addition, postnatal investigation of male and female progeny derived from control PBS and LPStreated Il10 ⁺ʹ⁺ and Il10 ⁻ʹ⁻ females from birth until 19 weeks of age showed that maternal LPS treatment constrains postnatal growth in male progeny regardless of maternal Il10 genotype, compared to male progeny… Advisors/Committee Members: Robertson, Sarah Anne (advisor), Thompson, Jeremy Gilbert E. (advisor), School of Paediatrics and Reproductive Health (school).

Subjects/Keywords: cytokines; pre-implantation embryo; inflammation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chin, P. Y. (2014). Cytokines and programming the pre-implantation embryo. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/92044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chin, Peck Yin. “Cytokines and programming the pre-implantation embryo.” 2014. Thesis, University of Adelaide. Accessed February 26, 2021. http://hdl.handle.net/2440/92044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chin, Peck Yin. “Cytokines and programming the pre-implantation embryo.” 2014. Web. 26 Feb 2021.

Vancouver:

Chin PY. Cytokines and programming the pre-implantation embryo. [Internet] [Thesis]. University of Adelaide; 2014. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2440/92044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chin PY. Cytokines and programming the pre-implantation embryo. [Thesis]. University of Adelaide; 2014. Available from: http://hdl.handle.net/2440/92044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brigham Young University

20. Clark, Daniel N. Promoter Polymorphisms in Interferon Regulatory Factor 5.

Degree: PhD, 2013, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5057&context=etd

► The promoter region of interferon regulatory factor 5 (IRF5) contains the rs2004640 T or G single nucleotide polymorphism (SNP) and a CGGGG indel. Both… (more)

▼ The promoter region of interferon regulatory factor 5 (IRF5) contains the rs2004640 T or G single nucleotide polymorphism (SNP) and a CGGGG indel. Both of these polymorphisms have been implicated as genetic risk factors for several autoimmune diseases, including systemic lupus erythematosus, whose pathology involves altered apoptosis and cytokine signaling. The polymorphisms' overall effect is to increase IRF5 levels. IRF5 is a transcription factor of several cytokines, including interferon, and is pro-apoptotic. Thus an alteration of cytokine levels and apoptosis signaling due to high IRF5 levels is the proposed source of autoimmune risk. Each of IRF5's four first exons (1A, 1B, 1C, 1D) has its own promoter and responds to specific stimuli. rs2004640 is a T or G polymorphism; T is the risk allele. The SNP creates a sequence-specific recognition site for the spliceosome, making exon 1B spliceable. Analysis of the 1B promoter showed putative p53 binding site. IRF5 and p53 are pro-apoptotic transcription factors, and the p53 site may provide a positive feedback loop. Apoptosis levels were altered in cells with the rs2004640 risk T/T allele when treated with DNA damaging agents (extrinsic apoptosis), but not when activating death receptors (intrinsic apoptosis). The 1B promoter was the only one to activate expression after inducing DNA damage in a luciferase reporter assay, and this activation was abolished after mutating the p53 site. The exon 1A promoter contains either three or four copies (4X) of CGGGG; the 4X variant is the risk allele. The 1A promoter is constitutively active and is responsive to the Toll-like receptor 7 agonist imiquimod. RNA folding analysis revealed a hairpin encompassing exon 1B. Mutational analysis showed that the hairpin shape decreased translation five-fold in a luciferase reporter assay. Cells with the CGGGG or rs2004640 risk allele exhibited higher levels of IRF5 mRNA and protein, but demonstrated no change in mRNA stability. Quantitative PCR in cell lines with either risk polymorphism demonstrated decreased usage of exons 1C or 1D, although no other correlated splicing events were observed. Also, several mRNA splice variants of IRF5 were sequenced. The risk polymorphisms altered cytokine signaling as well. Expression of interferon, Toll-like receptor, and B cell receptor pathways were affected by a risk haplotype which includes the rs2004640 SNP. The CGGGG polymorphism decreased the levels of CC-chemokine receptor 7. Specific transcription factor binding sites define promoter activity and thus first exon usage and transcription levels. Translation levels are affected by mRNA folding. Overall, the rs2004640 SNP and the CGGGG indel cause high levels of IRF5. High IRF5 expression causes altered cytokine and apoptosis signaling, and may bias the immune system toward autoimmunity.

Subjects/Keywords: autoimmunity; cytokines; apoptosis; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clark, D. N. (2013). Promoter Polymorphisms in Interferon Regulatory Factor 5. (Doctoral Dissertation). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5057&context=etd

Chicago Manual of Style (16^th Edition):

Clark, Daniel N. “Promoter Polymorphisms in Interferon Regulatory Factor 5.” 2013. Doctoral Dissertation, Brigham Young University. Accessed February 26, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5057&context=etd.

MLA Handbook (7^th Edition):

Clark, Daniel N. “Promoter Polymorphisms in Interferon Regulatory Factor 5.” 2013. Web. 26 Feb 2021.

Vancouver:

Clark DN. Promoter Polymorphisms in Interferon Regulatory Factor 5. [Internet] [Doctoral dissertation]. Brigham Young University; 2013. [cited 2021 Feb 26]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5057&context=etd.

Council of Science Editors:

Clark DN. Promoter Polymorphisms in Interferon Regulatory Factor 5. [Doctoral Dissertation]. Brigham Young University; 2013. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=5057&context=etd

Université Catholique de Louvain

21. Defour, Jean-Philippe. Thrombopoietin receptor : regulation, dimerization, oncogenic activation and bimodal signaling.

Degree: 2016, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/171096

►

Philadelphia negative myeloproliferative neoplasms include polycythemia vera, essential thrombocythemia and primary myelofibrosis. Those MPNs are clonal diseases for which the phenotype is almost always driven… (more)

Subjects/Keywords: TpoR; Mpl; Thrombopoietin; Dimerization; Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Defour, J. (2016). Thrombopoietin receptor : regulation, dimerization, oncogenic activation and bimodal signaling. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/171096

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Defour, Jean-Philippe. “Thrombopoietin receptor : regulation, dimerization, oncogenic activation and bimodal signaling.” 2016. Thesis, Université Catholique de Louvain. Accessed February 26, 2021. http://hdl.handle.net/2078.1/171096.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Defour, Jean-Philippe. “Thrombopoietin receptor : regulation, dimerization, oncogenic activation and bimodal signaling.” 2016. Web. 26 Feb 2021.

Vancouver:

Defour J. Thrombopoietin receptor : regulation, dimerization, oncogenic activation and bimodal signaling. [Internet] [Thesis]. Université Catholique de Louvain; 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2078.1/171096.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Defour J. Thrombopoietin receptor : regulation, dimerization, oncogenic activation and bimodal signaling. [Thesis]. Université Catholique de Louvain; 2016. Available from: http://hdl.handle.net/2078.1/171096

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queen Mary, University of London

22. Mittal, Gayatri Arvind. Development of a latent IL-17 antagonist for targeted therapy of rheumatoid arthritis.

Degree: PhD, 2012, Queen Mary, University of London

URL: http://qmro.qmul.ac.uk/xmlui/handle/123456789/8520 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667056

► Cytokine based therapies can be targeted to the sites of active inflammation by modifying a given cytokine as a LAP-cytokine. IL-17A has been shown to… (more)

▼ Cytokine based therapies can be targeted to the sites of active inflammation by modifying a given cytokine as a LAP-cytokine. IL-17A has been shown to directly contribute to pathogenesis of rheumatoid arthritis (RA). IL-17F, another member of the IL-17 cytokines family shares structural homology, receptor binding and biological properties with IL-17A but is 30-100 times less potent than IL-17A. (H161R) IL-17F mutant, a natural variant of IL-17F was shown to be protective against asthma in Japanese population. In vitro, IL-17F mutant competitively inhibited wild-type IL-17F and lacked the ability to activate downstream signaling pathways. I hypothesized that (H161R) IL-17F mutant is an additional inhibitor of IL-17A and if modified as LAP-IL-17F mutant, would be an effective targeted therapy for RA. (H161R) IL-17F mutant was created by substituting nucleotide A at position 485 in the wild type IL-17F by G. In vitro assays showed that the IL-17F mutant could bind to IL-17RC but lacked the ability to stimulate IL-6 secretion in HFFF2, 3T3 and HeLa cells and phosphorylate ERK1/2 in HeLa cells. IL-17F mutant also inhibited IL-17A induced secretion of IL-6 in all these cell lines. In order to assess in vivo therapeutic efficacy of LAP-IL-17F mutant in collagen induced arthritis mice, three mouse analogues of human IL-17F mutant were developed. Of these, (Q158R) IL-17F mutant displayed IL-17 agonistic properties, (H157R) IL-17F mutant could not be expressed in vitro and the truncated IL-17F mutant could not bind to mouse IL-17RC. Investigation of in vivo expression and pharmacokinetics of intravenous hydrodynamically delivered human full-length and LAP-IL-17 plasmid DNAs in naïve SCID and C57BL/6 mice showed that human IL-17 transgene expression was detectable in mouse serum at 48 hours post-delivery. The transgene expression however declined rapidly over the next two weeks. The local expression of transgene in C57BL/6 airpouch lavage fluid was less than 5% of its systemic levels. Taken together, the findings of the study warrant an investigation of in vivo therapeutic efficacy of human (H161F) IL-17F mutant in a suitable preclinical RA model, such as RA synovium/SCID mice.

Subjects/Keywords: 616.7; Medicine; Joints; Inflammation; Cytokines

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APA (6^th Edition):

Mittal, G. A. (2012). Development of a latent IL-17 antagonist for targeted therapy of rheumatoid arthritis. (Doctoral Dissertation). Queen Mary, University of London. Retrieved from http://qmro.qmul.ac.uk/xmlui/handle/123456789/8520 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667056

Chicago Manual of Style (16^th Edition):

Mittal, Gayatri Arvind. “Development of a latent IL-17 antagonist for targeted therapy of rheumatoid arthritis.” 2012. Doctoral Dissertation, Queen Mary, University of London. Accessed February 26, 2021. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8520 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667056.

MLA Handbook (7^th Edition):

Mittal, Gayatri Arvind. “Development of a latent IL-17 antagonist for targeted therapy of rheumatoid arthritis.” 2012. Web. 26 Feb 2021.

Vancouver:

Mittal GA. Development of a latent IL-17 antagonist for targeted therapy of rheumatoid arthritis. [Internet] [Doctoral dissertation]. Queen Mary, University of London; 2012. [cited 2021 Feb 26]. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/8520 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667056.

Council of Science Editors:

Mittal GA. Development of a latent IL-17 antagonist for targeted therapy of rheumatoid arthritis. [Doctoral Dissertation]. Queen Mary, University of London; 2012. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/8520 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667056

University of Technology, Sydney

23. Lawandi, J. Resistance of Melligen cells to pro-inflammatory cytokines involved in beta cell death.

Degree: 2010, University of Technology, Sydney

URL: http://hdl.handle.net/10453/20380

► It has been shown that stable transfection of insulin cDNA into the human liver cell line Huh7 resulted in synthesis, storage and regulated release of… (more)

▼ It has been shown that stable transfection of insulin cDNA into the human liver cell line Huh7 resulted in synthesis, storage and regulated release of insulin to a glucose stimulus (Huh7ins cells). However, Huh7ins cells responded to glucose at subphysiological levels (2.5mM) and in order to maintain normoglycaemia, insulin secretion in response to physiological levels of glucose is required. Consequently, the Huh7ins cells were further transfected with the human glucokinase gene to correct the skewed insulin secretory profile. The resulting cell line (Melligen) responds to glucose in the 4-5mM physiological range. If Melligen cells are to be used clinically to correct patient blood glucose concentrations, they need to be resistant to the toxic effects of pro-inflammatory cytokines responsible for the immune-mediated destruction of beta cells. Proinflammatory cytokines such as interferon-gamma (IFN-y), tumour necrosis factoralpha (TNF-a) and interleukin-1 beta (IL-1ß) play a major role in beta cell elimination during diabetes development. The aim of the present study was to determine if Melligen cells were resistant to the toxic effects of these cytokines. Cells were exposed to IFN-y (384ng/mL), TNF-a (10ng/mL) and IL-1ß (2ng/mL) for up to 10 days. Cell viability was measured using the Annexin V/propidium iodide (PI), PI and MTT cell viability assays. Insulin storage and chronic secretion were measured by radioimmunoassay. Acute insulin secretion was also determined by static incubations with increasing concentrations of 0, 1, 2, 2.5, 3, 3.5, 4, 4.5, 5 and 20mM glucose. Nitric oxide levels were assayed by the Griess reaction. The glucoseresponsive beta cell line, MIN-6, was used as a positive control throughout. Expression of the cytokine receptors IFNR1, IFNR2, IL1R1, IL1R2, TNFR1 and TNFR2 was determined in human pancreatic islet cells and the liver cell lines cultured in the absence of the cytokine treatment by RT-PCR. The quantitative analysis of the inhibitors of NF-kB (IkB-a, IkB-ß IkB-e) and NF-kB downstream effectors, iNOS, MCP-1 and Fas, was determined by real time RT-PCR in cytokine treated Huh7ins and Melligen cells with islet cells used as a positive control. Microarray analysis was used to determine which gene networks were being induced after 1h or 24h cytokine treatment of Melligen cells. Melligen cells were also tested for suitability for implantation. They were encapsulated (AustriaNova, Singapore) and insulin secretion and glucose responsiveness were determined. The cells were also treated with a single or double dose of 100µM or 20mM STZ to determine susceptibility to this beta cell toxin. The viability of MIN-6 cells was reduced after 2 days of culture with cytokines (P< 0.05). In contrast, the viability of Huh7, Huh7ins and Melligen cells was unaffected by cytokine treatment over 10 days. In addition to this, flow cytometry results corroborated the fact that there was no apoptotic cell death in these cell lines. The triple cytokines did not diminish chronic insulin secretion, storage or the…

Subjects/Keywords: Diabetes.; Cytokines.

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APA (6^th Edition):

Lawandi, J. (2010). Resistance of Melligen cells to pro-inflammatory cytokines involved in beta cell death. (Thesis). University of Technology, Sydney. Retrieved from http://hdl.handle.net/10453/20380

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lawandi, J. “Resistance of Melligen cells to pro-inflammatory cytokines involved in beta cell death.” 2010. Thesis, University of Technology, Sydney. Accessed February 26, 2021. http://hdl.handle.net/10453/20380.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lawandi, J. “Resistance of Melligen cells to pro-inflammatory cytokines involved in beta cell death.” 2010. Web. 26 Feb 2021.

Vancouver:

Lawandi J. Resistance of Melligen cells to pro-inflammatory cytokines involved in beta cell death. [Internet] [Thesis]. University of Technology, Sydney; 2010. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10453/20380.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lawandi J. Resistance of Melligen cells to pro-inflammatory cytokines involved in beta cell death. [Thesis]. University of Technology, Sydney; 2010. Available from: http://hdl.handle.net/10453/20380

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ontario Institute of Technology

24. McCarville, Justin. Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells.

Degree: 2012, University of Ontario Institute of Technology

URL: http://hdl.handle.net/10155/275

► Probiotics and prebiotics are emerging household terms, whose claimed health benefits share commonality. Their attributed health benefits include the production or induction of short chain… (more)

▼ Probiotics and prebiotics are emerging household terms, whose claimed health benefits share commonality. Their attributed health benefits include the production or induction of short chain fatty acids, maintaining bowel function, building colonization resistance (against pathogens) and treating antibiotic-associated diarrhea as well as colitis. Although both probiotic and prebiotic effects on immune system have been studied, the mechanisms of their activity are still not clearly defined and the conclusions drawn are elusive. While probiotics can act to influence the host at the cellular level, prebiotics, by definition, exert their effects indirectly through their impact on gut microbes. One purpose of this study was to investigate effects of Lactobacillus rhamnosus R0011 on innate immune parameters at the intestinal epithelial cell level, examining effects on both human and rat IEC. A second purpose was to define the effects of a range of prebiotic dietary fibres on the immune system at the mucosal and systemic level, using Biobreeding rats. L. rhamnosus demonstrated the ability to decrease proinflammatory cytokine and Toll-like receptor agonist-induced IL-8 and CINC-1 production from human and rat IEC, respectively. The timing of L. rhamnosus R0011 addition to HT-29 IEC, relative to proinflammatory challenge, influenced its ability to decrease IL-8 production. L. rhamnosus was more effective at decreasing production of IL-8 from human IEC when they were pre-incubated with this bacterium and subsequently challenged with proinflammatory stimuli. Certain effects of L. rhamnosus R011 were also observed in the absence of proinflammatory stimuli. Viable L. rhamnosus induced TNF-?? production from rat IEC and heat-killed L. rhamnosus decreased constitutive TGF-?? production from rat IEC and induced IL-8 or CINC-1 production from human and rat IEC, respectively. In Biobreeding rats, we demonstrated that oat dietary fibre significantly alters active TGF-??, CINC-1 and IL-6 levels in the colon in comparison to AIN-93G-fed rats. Wheat dietary fibre induced changes in active TGF-??, CINC-1 and IL-4 levels in the ileum in comparison to resistant starch-fed rats. Lastly, resistant starch exerted effects in the mesenteric lymph node, where changes in active TGF-?? were observed in rats in comparison to AIN-93G-fed rats. Oat bran, wheat bran and resistant starch had no effects on cytokine levels in the serum or spleen of rats. Fructooligosaccharide-fed rats had a significant increase in active TGF-?? levels in the colon and a significant decrease in active TGF-?? levels in the spleen. Overall this suggests a FOS supplemented diet has both mucosal and systemic effects in rats, while wheat, oat and resistant starch supplemented diets had effects focused at the different locations at the mucosal level. These results illustrate differences in the ability of different dietary fibres to target immune parameters in specific mucosal tissues along the gastrointestinal tract and differential ability to exert systemic effects.… Advisors/Committee Members: Green-Johnson, Julia.

Subjects/Keywords: Probiotics; Prebiotics; Microflora; GALT; Cytokines

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APA (6^th Edition):

McCarville, J. (2012). Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells. (Thesis). University of Ontario Institute of Technology. Retrieved from http://hdl.handle.net/10155/275

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCarville, Justin. “Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells.” 2012. Thesis, University of Ontario Institute of Technology. Accessed February 26, 2021. http://hdl.handle.net/10155/275.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCarville, Justin. “Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells.” 2012. Web. 26 Feb 2021.

Vancouver:

McCarville J. Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells. [Internet] [Thesis]. University of Ontario Institute of Technology; 2012. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10155/275.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCarville J. Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells. [Thesis]. University of Ontario Institute of Technology; 2012. Available from: http://hdl.handle.net/10155/275

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ontario Institute of Technology

25. Shastri, Padmaja. Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions.

Degree: 2015, University of Ontario Institute of Technology

URL: http://hdl.handle.net/10155/532

► Sex-based differences in immune parameters are well recognized, and recent evidence suggests differences in microbiota composition between sexes, necessitating assessment of effects of dietary fermentable… (more)

▼ Sex-based differences in immune parameters are well recognized, and recent evidence suggests differences in microbiota composition between sexes, necessitating assessment of effects of dietary fermentable material (DFM) in both males and females. Effects of consumption of DFM of differing composition (Wheat Bran (WB), Oat Bran (OB), Resistant Starch (RS), Fructooligosaccharides (FOS)) on the immune system were compared under resting and immunized conditions between sexes. The gut microbiota composition and short chain fatty acid profiles differed between rats fed different DFM and also differed between sexes following FOS consumption. Immune parameters were analyzed for the effect of diet, differences between sexes or interactions (diet x sex), to detect the effect of DFM on male and female rats. The kinetics of primary (Day 0, 5, 10, 15) and secondary antibody responses (Day 21) were measured following immunization with a T cell-dependent antigen, Keyhole Limpet Hemocyanin (KLH). Under resting conditions, male rats had more FoxP3+ Treg cells in the mesenteric lymph node (MLN) and spleen than did females. Levels of the regulatory cytokine TGF-??1 also increased in the MLN in a DFM-dependent manner. In contrast, percentages of MLN and splenic CD68+ macrophages and levels of gut tissue IL-10 were higher in females than males. These results suggest immune regulation at the mucosal level is controlled in a differential manner between sexes and was affected by DFM intake only in males. At the systemic level, DFM intake delayed the primary anti-KLH IgG antibody response kinetics in male (control>>WB>OB>RS>FOS) and female (control> FOS>WB>RS>OB) rats. The secondary anti-KLH IgG response in control-fed males was 10-fold higher than in control-fed females. DFM intake in males significantly decreased anti-KLH IgG levels relative to control-fed males. Collectively, these findings demonstrate DFM consumption in males potentially induces systemic immune regulation leading to a delayed response to immune challenge, an effect that was not observed in females. Advisors/Committee Members: Green-Johnson, Julia.

Subjects/Keywords: Cytokines; Immunoglobulins; Microbiota; Sex; Fibre

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APA (6^th Edition):

Shastri, P. (2015). Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions. (Thesis). University of Ontario Institute of Technology. Retrieved from http://hdl.handle.net/10155/532

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shastri, Padmaja. “Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions.” 2015. Thesis, University of Ontario Institute of Technology. Accessed February 26, 2021. http://hdl.handle.net/10155/532.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shastri, Padmaja. “Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions.” 2015. Web. 26 Feb 2021.

Vancouver:

Shastri P. Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions. [Internet] [Thesis]. University of Ontario Institute of Technology; 2015. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10155/532.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shastri P. Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions. [Thesis]. University of Ontario Institute of Technology; 2015. Available from: http://hdl.handle.net/10155/532

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

26. Liu, Yu. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.

Degree: School of Medicine and Dentistry., 2008, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer

Subjects/Keywords: Macrophages.; Cytokines.

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APA (6^th Edition):

Liu, Y. (2008). The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Liu, Yu. “The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.” 2008. Doctoral Dissertation, University of Aberdeen. Accessed February 26, 2021. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Liu, Yu. “The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.” 2008. Web. 26 Feb 2021.

Vancouver:

Liu Y. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. [Internet] [Doctoral dissertation]. University of Aberdeen; 2008. [cited 2021 Feb 26]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer.

Council of Science Editors:

Liu Y. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. [Doctoral Dissertation]. University of Aberdeen; 2008. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer

University of Southern California

27. Tam, Yvonne. Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics.

Degree: MS, Cranio-Facial Biology, 2012, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1300

► Background: Some investigators have detected edema, swelling and histological changes in the soft tissue under pontics, while others have demonstrated that exceptional plaque control can… (more)

▼ Background: Some investigators have detected edema, swelling and histological changes in the soft tissue under pontics, while others have demonstrated that exceptional plaque control can lead to healthy soft tissue at pontic sites. A new pontic design by Kim et al. (2009) attempts to address the issue of esthetics and oral hygiene by creating a ridge lap pontic with circumferential pressure, and was hypothesized to be associated with favorable microbiological and inflammatory profiles. ❧ Objective: The aim of this study was to explore the microbiological and cytokine/chemokine profiles of implants as well as the “pressure pontic” site in implantsupported fixed partial dentures and contrast them to that of healthy teeth. ❧ Materials and Methods: Gingival crevicular fluid was sampled from 6 patients, each with an implant-supported fixed prosthesis and a healthy tooth. One implant, the adjacent pontic site, and the contralateral tooth from the implant sampled were sampled whenever possible with Periopaper strips and subgingival plaque was collected with paper points. Both were placed separately into the peri-implant sulcus, periodontal sulcus as well as the pontic-mucosa interface on the mesio- and disto-lingual surfaces. Microbial samples were analyzed for periodontopathic bacteria via selective anaerobic culture techniques. Proinflammatory cytokines were quantified by flow cytometry analysis of GCF with two separate kits. The first kit tested for IL-8, IL-6, IL-1β, TNF-α, and IL-10 while the second one detected the concentration of IL-8, IP-10, RANTES, MCP-1, and MIG. ❧ Results: The number of sites along with the cultivable levels of periodontopathic bacteria detected in pontic sites, implant sulci, and periodontal sulci were similar. There were no significant differences detected in cytokines and chemokines due to a wide range of values and limited number of samples. The results of the chemokine values for patients 5 and 6, who were sampled after one month, appeared higher than those of patients 1-4, who were sampled one year after implant restoration. ❧ Conclusion: The ridge lap pontic with circumferential pressure merits further evaluation. The microbiological profile of the pontic site was comparable to that of other pontic research designs observed by other researchers (Wang et al. 1998). The pontic area also provided esthetic results, a lack of clinical inflammation, as well as a decrease in immune response over time. Advisors/Committee Members: Zadeh, Homah (Committee Chair), Rich, Sandra (Committee Member), Navazesh, Mahvash (Committee Member).

Subjects/Keywords: cytokines; chemokines; implants; pontics

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APA (6^th Edition):

Tam, Y. (2012). Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1300

Chicago Manual of Style (16^th Edition):

Tam, Yvonne. “Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics.” 2012. Masters Thesis, University of Southern California. Accessed February 26, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1300.

MLA Handbook (7^th Edition):

Tam, Yvonne. “Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics.” 2012. Web. 26 Feb 2021.

Vancouver:

Tam Y. Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics. [Internet] [Masters thesis]. University of Southern California; 2012. [cited 2021 Feb 26]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1300.

Council of Science Editors:

Tam Y. Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics. [Masters Thesis]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1300

28. Prens, Errol. The immunopathophysiology of psoriasis : studies on accessory cells, cytokines and their receptors.

Degree: 1992, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/39776

► textabstractThe above data show that prodigious alterations occur in the expression of cytokines and their receptors in psoriasis. Although a crucial role lor cytokines in… (more)

Subjects/Keywords: cytokines; psoriasis

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APA (6^th Edition):

Prens, E. (1992). The immunopathophysiology of psoriasis : studies on accessory cells, cytokines and their receptors. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/39776

Chicago Manual of Style (16^th Edition):

Prens, Errol. “The immunopathophysiology of psoriasis : studies on accessory cells, cytokines and their receptors.” 1992. Doctoral Dissertation, Erasmus University Medical Center. Accessed February 26, 2021. http://hdl.handle.net/1765/39776.

MLA Handbook (7^th Edition):

Prens, Errol. “The immunopathophysiology of psoriasis : studies on accessory cells, cytokines and their receptors.” 1992. Web. 26 Feb 2021.

Vancouver:

Prens E. The immunopathophysiology of psoriasis : studies on accessory cells, cytokines and their receptors. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1992. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1765/39776.

Council of Science Editors:

Prens E. The immunopathophysiology of psoriasis : studies on accessory cells, cytokines and their receptors. [Doctoral Dissertation]. Erasmus University Medical Center; 1992. Available from: http://hdl.handle.net/1765/39776

University of Waterloo

29. Pervaiz Munir, Nabeel. Effects of acute exercise and voluntary freewheel exercise in mice on pro-inflammatory cytokines and markers of apoptosis in the hippocampus.

Degree: 2011, University of Waterloo

URL: http://hdl.handle.net/10012/6231

► Introduction: Alzheimer’s disease (AD) and dementias constitute a significant public health burden and it is estimated that one in 85 people may be living with… (more)

▼ Introduction: Alzheimer’s disease (AD) and dementias constitute a significant public health burden and it is estimated that one in 85 people may be living with AD by 2050. Dementias are a spectrum of diseases with common traits including amyloid protein growth, neurodegradation, neurofibrillary plaque and tangle formation, and which may be influenced by pro- and anti- inflammatory immune mechanisms. Even a modest delay in onset could result in significant reductions in the social and economic burdens of dementias. An important lifestyle factor identified in risk reduction is physical activity (PA). Although the association between dementia risk and PA has been established, the exact physiological mechanisms through which protection occurs are not known. This research consists of two experiments that were designed to explore the effects of physical activity on pro- and anti-inflammatory cytokines and apoptosis in the mouse hippocampus, a brain region implicated in learning, memory, and cognition. Methods: Study1: Female C57BL/6 mice, 4-5 months of age, were divided into three groups: sedentary controls (NOTREAD) (n = 22), treadmill exercise with immediate sacrifice (TREAD-Imm) (n = 21), or treadmill exercise with sacrifice after 2 hours (TREAD-2h) (n = 20). TNF-α, IL-6, and IL-1β expression in the hippocampus and intestinal lymphocytes were measured by Western blot analysis. Percentages of hippocampal cells undergoing apoptosis (Annexin+) or necrosis (Propidium Iodide+) were determined through flow cytometry. Plasma levels of 8-isoprostane and corticosterone were measured using commercially available EIA kits. Study # 2: Female C57BL/6 mice, 3-4 weeks of age, were assigned to wheel running (WR; n = 20) or a control condition (No WR; n = 22) and sacrificed after the 16 weeks. Data collected included measures of training status (running volume, body weight, run-to-exhaustion time, and skeletal muscle cytochrome c oxidase activity), flow cytometric analysis of hippocampal cell phenotypes and apoptosis (CD45+, CD11b+, Annexin+, Annexin+/PI+, PI+), and cytokine concentrations (TNF-α, IL-1β, IL-12, IL-6, IL-1ra, and IL-10) in cell lysates. Results: Study #1: Acute treadmill exercise lead to significant decreases in TNF-α (p<0.05) and increases in IL-6 (p<0.05) expression in the hippocampus of healthy mice. No effects of acute exercise on the apoptotic status of hippocampal cells were observed. In intestinal lymphocytes, the exercise bout lead to significant increases in TNF-α (p<0.05), IL-6 (p<0.05), and IL-1β (p<0.05). Acute exercise was associated with a significant increase in both plasma 8-isoprostane (p<0.05) and corticosterone (p<0.05) levels. Study #2: WR mice had measurable training effects and significantly lower TNF-α (p<0.05) and higher IL-6 (p<0.05), IL-1ra (p<0.05) and IL-12 (p<0.05) expression in the hippocampus compared to controls. IL-1β, IL-10, and the percent of apoptotic, dead cells, and cell phenotypes did not change due to training. Conclusion: Exercise chronicity (acute vs. chronic), stress…

Subjects/Keywords: exercise; hippocampus; cytokines; training

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APA (6^th Edition):

Pervaiz Munir, N. (2011). Effects of acute exercise and voluntary freewheel exercise in mice on pro-inflammatory cytokines and markers of apoptosis in the hippocampus. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/6231

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pervaiz Munir, Nabeel. “Effects of acute exercise and voluntary freewheel exercise in mice on pro-inflammatory cytokines and markers of apoptosis in the hippocampus.” 2011. Thesis, University of Waterloo. Accessed February 26, 2021. http://hdl.handle.net/10012/6231.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pervaiz Munir, Nabeel. “Effects of acute exercise and voluntary freewheel exercise in mice on pro-inflammatory cytokines and markers of apoptosis in the hippocampus.” 2011. Web. 26 Feb 2021.

Vancouver:

Pervaiz Munir N. Effects of acute exercise and voluntary freewheel exercise in mice on pro-inflammatory cytokines and markers of apoptosis in the hippocampus. [Internet] [Thesis]. University of Waterloo; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10012/6231.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pervaiz Munir N. Effects of acute exercise and voluntary freewheel exercise in mice on pro-inflammatory cytokines and markers of apoptosis in the hippocampus. [Thesis]. University of Waterloo; 2011. Available from: http://hdl.handle.net/10012/6231

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Ρεγκλή - Γκούμα, Αρετή. Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα.

Degree: 2004, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/13745

Subjects/Keywords: Κυτταροκίνες; Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ρεγκλή - Γκούμα, . �. (2004). Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/13745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ρεγκλή - Γκούμα, Αρετή. “Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα.” 2004. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed February 26, 2021. http://hdl.handle.net/10442/hedi/13745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ρεγκλή - Γκούμα, Αρετή. “Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα.” 2004. Web. 26 Feb 2021.

Vancouver:

Ρεγκλή - Γκούμα �. Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2004. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10442/hedi/13745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ρεγκλή - Γκούμα �. Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2004. Available from: http://hdl.handle.net/10442/hedi/13745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations