subject:(Cytokines)

1. Reuter, Simone. Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia.

Degree: Docteur es, Biologie Cellulaire et Nutrition, 2008, Université Henri Poincaré – Nancy I

URL: http://www.theses.fr/2008NAN10054

► La??-glutamyltransférase (GGT) est une enzyme membranaire qui catalyse l’hydrolyse du glutathion, le principal antioxydant cellulaire. Cette enzyme est surexprimée dans de nombreux cancers (p.ex. leucémies)… (more)

▼ La??-glutamyltransférase (GGT) est une enzyme membranaire qui catalyse l’hydrolyse du glutathion, le principal antioxydant cellulaire. Cette enzyme est surexprimée dans de nombreux cancers (p.ex. leucémies) et appartient à une famille multigénique d’au moins treize gènes. Le gène I, qui code pour la protéine active, est exprimé en trois ARNm (A, B et C), montrant tous la même phase de lecture ouverte mais différant dans leur région 5’ non traduite. A côté de l’implication de la GGT dans le développement des cancers, elle intervient également dans l’acquisition des résistances aux traitements anticancéreux et dans la synthèse des leukotriènes. Au cours de cette thèse, nous nous sommes intéressés aux mécanismes transcriptionnels qui régulent l’expression du gène I de la GGT. Plus particulièrement, nous avons étudié la régulation de la GGT après traitement des cellules K562 avec l’ester de phorbol, TPA, et la cytokine pro-inflammatoire, TNF?? Ces deux substances activent les facteurs de transcription AP-1 et NF-?B, qui sont connus pour réguler des gènes impliqués dans le développement des chimiorésistances. Nos résultats montrent que les deux promoteurs B et C de la GGT ainsi que les trois ARNm A, B et C, la protéine GGT ainsi que l’activité enzymatique de la GGT sont induits par le TNF?, mais pas par le TPA, dans la lignée cellulaire K562, qui est établie à partir de cellules issues d’une leucémie myéloïde chronique. Vu que l’induction du promoteur C de la GGT par le TNF? est fortement diminuée avec la curcumine, un agent chimiopréventif utilisé comme inhibiteur de la voie de signalisation NF-?B, nous avons émis une première hypothèse selon laquelle le facteur de transcription NF-?B serait impliqué dans la régulation du promoteur C de la GGT dans les cellules K562. Afin de prouver notre hypothèse nous avons utilisé des siRNA ciblant spécifiquement les deux sous-unités les plus représentées de NF-?B, p50 et p65, et effectivement l’induction du promoteur C de la GGT par le TNF? est inhibée par ces siRNA. Des expériences par co-transfection avec des protéines de la voie NF-?B, TRAF2, TRADD, I?B? et p65, confirment davantage cette hypothèse selon laquelle le promoteur C de la GGT est régulé par la voie de signalisation NF-?B dans les cellules K562. Des expériences par mutation ont ensuite permis d’identifier le site sur l’ADN qui est responsable de l’induction du promoteur C de la GGT par le TNF? dans les cellules K562. Ce site, localisé de -111 à -123 par rapport au site d’initiation de la transcription, est au moins capable de fixer le facteur de transcription p50 NF-?B. A proximité de ce site, nous avons identifié un autre site fonctionnel, localisé de - 73 à – 92 par rapport au site d’initiation de la transcription, qui lie le facteur de transcription Sp1. Des analyses par chromatine immunoprécipitation (ou ChIP) ont confirmé la liaison des facteurs de transcription NF-?B et Sp1 sur le promoteur C de la GGT et montrent de plus que l’ARN polymérase II est également recruté à cet endroit précis du promoteur. En outre,… Advisors/Committee Members: Diederich, Marc (thesis director).

Subjects/Keywords: Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reuter, S. (2008). Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia. (Doctoral Dissertation). Université Henri Poincaré – Nancy I. Retrieved from http://www.theses.fr/2008NAN10054

Chicago Manual of Style (16^th Edition):

Reuter, Simone. “Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia.” 2008. Doctoral Dissertation, Université Henri Poincaré – Nancy I. Accessed February 24, 2020. http://www.theses.fr/2008NAN10054.

MLA Handbook (7^th Edition):

Reuter, Simone. “Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia.” 2008. Web. 24 Feb 2020.

Vancouver:

Reuter S. Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia. [Internet] [Doctoral dissertation]. Université Henri Poincaré – Nancy I; 2008. [cited 2020 Feb 24]. Available from: http://www.theses.fr/2008NAN10054.

Council of Science Editors:

Reuter S. Étude de l’effet de la cytokine TNFa sur la régulation de l’expression de la ?-glutamyl-transférase humaine : Effect of TNF on the regulation of -glutamyltransferase in human leukemia. [Doctoral Dissertation]. Université Henri Poincaré – Nancy I; 2008. Available from: http://www.theses.fr/2008NAN10054

McGill University

2. Balasingam, Vijayabalan. Cytokines and Astroglial Reactivity.

Degree: PhD, Department of Neurology and Neurosurgery, 1995, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile162006.pdf

►

Note:

Astroglial reactivity is a prominent universal event following trauma and inflammation to the adult central nervous system. In contrast, injury inflicted during early postnatal… (more)

Subjects/Keywords: Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balasingam, V. (1995). Cytokines and Astroglial Reactivity. (Doctoral Dissertation). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile162006.pdf

Chicago Manual of Style (16^th Edition):

Balasingam, Vijayabalan. “Cytokines and Astroglial Reactivity.” 1995. Doctoral Dissertation, McGill University. Accessed February 24, 2020. http://digitool.library.mcgill.ca/thesisfile162006.pdf.

MLA Handbook (7^th Edition):

Balasingam, Vijayabalan. “Cytokines and Astroglial Reactivity.” 1995. Web. 24 Feb 2020.

Vancouver:

Balasingam V. Cytokines and Astroglial Reactivity. [Internet] [Doctoral dissertation]. McGill University; 1995. [cited 2020 Feb 24]. Available from: http://digitool.library.mcgill.ca/thesisfile162006.pdf.

Council of Science Editors:

Balasingam V. Cytokines and Astroglial Reactivity. [Doctoral Dissertation]. McGill University; 1995. Available from: http://digitool.library.mcgill.ca/thesisfile162006.pdf

3. Patrani, Maria. Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή.

Degree: 2016, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/38746

►

Purpose: Although soluble urokinase plasminogen activator receptor (suPAR) is avalidated as a useful prognostic marker of sepsis outcome, its usefulness has neverbeen studied in patients… (more)

▼

Purpose: Although soluble urokinase plasminogen activator receptor (suPAR) is avalidated as a useful prognostic marker of sepsis outcome, its usefulness has neverbeen studied in patients with multiple traumas.Methods: Serum suPAR was measured in 85 patients with systemic inflammatoryresponse syndrome admitted in the Intensive Care Unit (ICU) due to multiple injurieswith injury severity index ≥15 without infection. Measurements were repeated afterfour days or at sepsis onset. The primary endpoint was the validity of suPAR toprognosticate development of multiple organ failure (MOF).Results: suPAR greater than or equal to 8ng/ml had 35.3% sensitivity, 84.5%specificity, 57.1% positive predictive value and 88.2% negative predictive value toindicate the development of MOF. This was confirmed by logistic regression analysis.Any over- time increase of suPAR more than or equal to 40% from the baselineadmission levels was associated with odds ratio 9.33 (p: 0.047) for the development of severe sepsis. The specificity of this change for the diagnosis of severe sepsiswas 90.0% and the positive predictive value 80.0%.Conclusions: suPAR is an accurate and independent biomarker for the prognosis ofthe development of MOF in critically ill non-septic patients admitted with multipletraumas in the ICU.Increase more than 40% from baseline admission levels isdiagnostic of the progression into severe sepsis

Σκοπός: Αν και o διαλυτός υποδοχέας του ενεργοποιητή του πλασμινογόνου τύπουουροκινάσης (suPAR) έχει επικυρωθεί ως χρήσιμος προγνωστικός δείκτης τηςέκβασης στη σήψη, η χρησιμότητά του δεν έχει ποτέ μελετηθεί σε ασθενείς μεπολλαπλούς τραυματισμούς.Μέθοδος: Ο suPAR μετρήθηκε στον ορό 85 ασθενών με σύνδρομο συστηματικήςφλεγμονώδους αντίδρασης που έκαναν εισαγωγή στη Μ.Ε.Θ. λόγω πολλαπλώντραυματισμών με δείκτη βαρύτητας τραύματος (ISS) ≥ 15 χωρίς παρουσία λοίμωξης.Οι μετρήσεις επαναλαμβάνονταν μετά από τέσσερις ημέρες ή κατά την έναρξη τηςσήψης. Ο βασικός στόχος ήταν η τεκμηρίωση της εγκυρότητας του suPAR στηνπρόγνωση εμφάνισης πολυοργανικής ανεπάρκειας.Αποτελέσματα: Τιμή suPAR ≥ 8ng/ml είχε 35,3% ευαισθησία, 84,5% ειδικότητα,57,1% θετική προγνωστική αξία και 88,2% αρνητική προγνωστική αξία να υποδείξειτην εμφάνιση πολυοργανικής ανεπάρκειας. Αυτό επιβεβαιώθηκε με ανάλυσηλογιστικής παλινδρόμησης. Με την πάροδο του χρόνου οποιαδήποτε αύξηση τουsuPAR ≥ 40% από τα επίπεδα αναφοράς κατά την εισαγωγή συνδυάστηκε με OR9,33 (p: 0,047) για την εμφάνιση σοβαρής σήψης. Η ειδικότητα αυτής της μεταβολήςγια τη διάγνωση σοβαρής σήψης ήταν 90% και η θετική προγνωστική αξία 80%.Συμπέρασμα: Ο suPAR είναι ένας ακριβής και ανεξάρτητος βιολογικός δείκτης γιατην πρόγνωση εμφάνισης πολυοργανικής ανεπάρκειας σε βαρέως πάσχοντες μησηπτικούς ασθενείς που έκαναν εισαγωγή στη Μ.Ε.Θ. με πολλαπλούςτραυματισμούς. Αύξηση > 40% από τα επίπεδα αναφοράς κατά την εισαγωγή είναι διαγνωστική για την εξέλιξη σε σοβαρή σήψη

Subjects/Keywords: Κυτταροκίνες; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Patrani, M. (2016). Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/38746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Patrani, Maria. “Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή.” 2016. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed February 24, 2020. http://hdl.handle.net/10442/hedi/38746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Patrani, Maria. “Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή.” 2016. Web. 24 Feb 2020.

Vancouver:

Patrani M. Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/10442/hedi/38746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Patrani M. Μεταβολές της κινητικής των προ- και αντι-φλεγμονωδών κυτταροκινών του ορού κατά την εξέλιξη του πολυτραυματία σε σηπτικό ασθενή. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. Available from: http://hdl.handle.net/10442/hedi/38746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oklahoma

4. Chollangi, Srinivas. STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M.

Degree: PhD, 2009, University of Oklahoma

URL: http://hdl.handle.net/11244/319327

► Oncostatin M (OSM) is a pleiotropic cytokine that belongs to the interleukin-6 (IL-6) family of cytokines. These cytokines play crucial role in diverse biological events… (more)

▼ Oncostatin M (OSM) is a pleiotropic cytokine that belongs to the interleukin-6 (IL-6) family of cytokines. These cytokines play crucial role in diverse biological events like inflammation, neuroprotection, hematopoiesis, metabolism and development. For example, when exposed to moderate levels of oxidative stress (i.e. bright cyclic light), IL-6 family cytokines are strongly upregulated in the retina. Using inhibition studies, we show that these upregulated cytokines play an essential role in protecting the neuronal cells from succumbing to subsequent lethal doses of oxidative stress. This wide range of functions elicited by IL-6 family cytokines are mediated by the formation of multimeric receptor complexes, which include a common receptor glycoprotein 130 (gp130). OSM is unique in this family since it can signal via two different receptor complexes i.e. LIFR:gp130 (type I) and OSMR:gp130 (type II). We have identified a unique helical loop on OSM between its B and C helices, which is not found on other IL-6 family cytokines. Based on its size and location, we hypothesized that the BC loop is responsible for OSM's unique ability to bind and activate OSMR. However, our experiments with mutated OSM molecules that have truncated BC loops showed that the BC loop does not play a role in OSM's unique ability to bind OSMR, but instead presents a steric hindrance for OSM's strong interaction with OSMR and LIFR. Kinetic and equilibrium binding analyses using surface plasmon resonance (SPR) and enzyme-linked immunosorbent assay (ELISA) support the evidence for improved binding and stability between mutant OSMs and the receptor complexes. Also, as a consequence of improved binding, these structurally modified OSMs exhibit enhanced ability in suppressing the proliferation of A375 melanoma cells and also protecting the neuronal cells in retina from oxidative stress, demonstrating that improved binding has functional consequences. Finally, we have evaluated the applicability of poly(ethylene glycol) (PEG) based hydrogels for delivering these molecules in a controlled manner. Using in vitro and in vivo studies in mice, we show that PEG hydrogels fabricated with PEG-diacrylate macromers with a molecular weight of 5000 Da can be used to deliver these cytokines in a sustained manner for ~12 days. And, for transscleral transport, a release rate of at least 0.5 ug/day is required for OSM to cross the scleral barriers and reach the retina to induce STAT3 activation. Advisors/Committee Members: Nollert, Mathias U||Ash, John D (advisor).

Subjects/Keywords: Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chollangi, S. (2009). STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/319327

Chicago Manual of Style (16^th Edition):

Chollangi, Srinivas. “STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M.” 2009. Doctoral Dissertation, University of Oklahoma. Accessed February 24, 2020. http://hdl.handle.net/11244/319327.

MLA Handbook (7^th Edition):

Chollangi, Srinivas. “STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M.” 2009. Web. 24 Feb 2020.

Vancouver:

Chollangi S. STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M. [Internet] [Doctoral dissertation]. University of Oklahoma; 2009. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/11244/319327.

Council of Science Editors:

Chollangi S. STRUCTURE-FUNCTION ANALYSIS OF HUMAN ONCOSTATIN M. [Doctoral Dissertation]. University of Oklahoma; 2009. Available from: http://hdl.handle.net/11244/319327

University of Adelaide

5. Gameau, Louise J. (Louise Joy). The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau.

Degree: 1998, University of Adelaide

URL: http://hdl.handle.net/2440/19442

► The purpose of this project was to examine the cellular and molecular processes involved in expression of embryonic signals and the initial interactions that occur… (more)

Subjects/Keywords: Cytokines.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gameau, L. J. (. J. (1998). The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/19442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gameau, Louise J (Louise Joy). “The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau.” 1998. Thesis, University of Adelaide. Accessed February 24, 2020. http://hdl.handle.net/2440/19442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gameau, Louise J (Louise Joy). “The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau.” 1998. Web. 24 Feb 2020.

Vancouver:

Gameau LJ(J. The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau. [Internet] [Thesis]. University of Adelaide; 1998. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/2440/19442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gameau LJ(J. The effect of cytokines on chorionic gonadotrophin expression in the marmoset monkey embryo / Louise J. Gameau. [Thesis]. University of Adelaide; 1998. Available from: http://hdl.handle.net/2440/19442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

6. 王熙喬; Wong, Hei-kiu. Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors.

Degree: Master of Medical Sciences, 2009, University of Hong Kong

URL: Wong, H. [王熙喬]. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4260999 ; http://dx.doi.org/10.5353/th_b4260999 ; http://hdl.handle.net/10722/55959

published_or_final_version

Biochemistry

Master

Master of Medical Sciences

Subjects/Keywords: Cytokines.; Cytokines - Receptors.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

王熙喬; Wong, H. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Masters Thesis). University of Hong Kong. Retrieved from Wong, H. [王熙喬]. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4260999 ; http://dx.doi.org/10.5353/th_b4260999 ; http://hdl.handle.net/10722/55959

Chicago Manual of Style (16^th Edition):

王熙喬; Wong, Hei-kiu. “Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors.” 2009. Masters Thesis, University of Hong Kong. Accessed February 24, 2020. Wong, H. [王熙喬]. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4260999 ; http://dx.doi.org/10.5353/th_b4260999 ; http://hdl.handle.net/10722/55959.

MLA Handbook (7^th Edition):

王熙喬; Wong, Hei-kiu. “Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors.” 2009. Web. 24 Feb 2020.

Vancouver:

王熙喬; Wong H. Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. [Internet] [Masters thesis]. University of Hong Kong; 2009. [cited 2020 Feb 24]. Available from: Wong, H. [王熙喬]. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4260999 ; http://dx.doi.org/10.5353/th_b4260999 ; http://hdl.handle.net/10722/55959.

Council of Science Editors:

王熙喬; Wong H. Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. [Masters Thesis]. University of Hong Kong; 2009. Available from: Wong, H. [王熙喬]. (2009). Functional roles of rarely-used synonymous codon and sequences variation in type I cytokine receptors. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4260999 ; http://dx.doi.org/10.5353/th_b4260999 ; http://hdl.handle.net/10722/55959

University of Aberdeen

7. Liu, Yu. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.

Degree: 2008, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=24848 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493453

► Macrophages (M?) are widely distributed immune cells and can be phenotypically polarised by microenvironment to mount specific functional programs. M? polarised in vitro can be… (more)

Subjects/Keywords: 616.07995; Macrophages : Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2008). The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=24848 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493453

Chicago Manual of Style (16^th Edition):

Liu, Yu. “The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.” 2008. Doctoral Dissertation, University of Aberdeen. Accessed February 24, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=24848 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493453.

MLA Handbook (7^th Edition):

Liu, Yu. “The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.” 2008. Web. 24 Feb 2020.

Vancouver:

Liu Y. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. [Internet] [Doctoral dissertation]. University of Aberdeen; 2008. [cited 2020 Feb 24]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=24848 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493453.

Council of Science Editors:

Liu Y. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. [Doctoral Dissertation]. University of Aberdeen; 2008. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=24848 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493453

University of Hong Kong

8. Chan, Yat-yan. An investigation of the action of cytokines on human endometrial and endometriotic colony forming units.

Degree: M. Phil., 2011, University of Hong Kong

URL: Chan, Y. [陳溢恩]. (2011). An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807994 ; http://dx.doi.org/10.5353/th_b4807994 ; http://hdl.handle.net/10722/161589

► Cyclic proliferation and differentiation occur in the human endometrium in each menstrual cycle. Aside from the precise regulation of estrogen and progesterone, the physiology of… (more)

▼ Cyclic proliferation and differentiation occur in the human endometrium in each menstrual cycle. Aside from the precise regulation of estrogen and progesterone, the physiology of the human endometrium is also tightly regulated by cytokines. It is therefore not surprising that imbalance of cytokine expression could be observed when women suffer from gynecological disease such as endometriosis, a common gynecological disease associated with altered cytokine profile and chronic inflammatory response. Although the etiology of endometriosis is not yet well elucidated, a commonly accepted Sampson theory suggests reflux of endometrial tissue to other parts of the reproductive tract would be one of the causes. As recent findings demonstrate the presence of somatic stem cells residing in human endometrium and endometriotic cyst, the hypothesis of the thesis was that cytokines confer a regulatory role in the proliferation and self-renewal of the endometrial and endometriotic stem/progenitor cells. In this project the following objectives were studied: 1) To investigate the effect of cytokines interleukin (IL) - 1β, IL-8, IL-10, IL-13, tumor necrosis factor (TNF) – α and interferon (IFN) – γ on the clonogenic ability of endometrial and endometriotic colony forming units (CFUs); 2) To determine the effect of IL-1β and IL-13 on the self-renewal and proliferative potential of the CFUs; 3) To elucidate the expression patterns of IL-1 and IL-13 receptors in endometrial and endometriotic sections and CFUs. Clonogenic analysis of endometrial and endometriotic stem cells showed an increase in clonogenicity in endometrial epithelial cells when treated with IL-1β. Treatment of IL-13 led to a drop in clonogenicity in endometrial epithelial and stromal cells while IFN-γ treatment resulted in a decreased clonogenicity in endometrial epithelial, stromal and endometriotic stromal cells. Other cytokines (IL-8, IL-10, TNF-α) displayed no effect on the clonogenicity of endometrial and endometriotic cells. Functional study by replating IL-1β and IL-13 treated endometrial and endometriotic CFUs revealed that these cytokines did not affect the self-renewal ability of endometrial and endometriotic CFUs. The proliferative potential of CFUs was determined by total cell output assay. The results suggested that IL-1β up-regulated the proliferative potential of the endometriotic stromal CFUs but not the endometrial epithelial and stromal CFUs, while IL-13 did not alter the proliferative potentials of endometrial and endometriotic CFUs. Comparative analysis on the effect of IL-1β and IL-13 between endometrial and endometriotic stromal CFUs demonstrated that IL-1β would preferentially promote the proliferative potential of ectopic stromal CFUs while IL-13 selectively increases that of normal stromal CFUs. Receptor expression analysis by immunostaining demonstrated the presence of IL-1 receptor and IL-13 receptors protein in the glandular epithelium of endometrial tissue. Their expression was more diffuse in the endometriotic tissues. Using reverse… Advisors/Committee Members: Yeung, WSB.

Subjects/Keywords: Endometrium.; Endometriosis.; Cytokines.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chan, Y. (2011). An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. (Masters Thesis). University of Hong Kong. Retrieved from Chan, Y. [陳溢恩]. (2011). An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807994 ; http://dx.doi.org/10.5353/th_b4807994 ; http://hdl.handle.net/10722/161589

Chicago Manual of Style (16^th Edition):

Chan, Yat-yan. “An investigation of the action of cytokines on human endometrial and endometriotic colony forming units.” 2011. Masters Thesis, University of Hong Kong. Accessed February 24, 2020. Chan, Y. [陳溢恩]. (2011). An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807994 ; http://dx.doi.org/10.5353/th_b4807994 ; http://hdl.handle.net/10722/161589.

MLA Handbook (7^th Edition):

Chan, Yat-yan. “An investigation of the action of cytokines on human endometrial and endometriotic colony forming units.” 2011. Web. 24 Feb 2020.

Vancouver:

Chan Y. An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. [Internet] [Masters thesis]. University of Hong Kong; 2011. [cited 2020 Feb 24]. Available from: Chan, Y. [陳溢恩]. (2011). An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807994 ; http://dx.doi.org/10.5353/th_b4807994 ; http://hdl.handle.net/10722/161589.

Council of Science Editors:

Chan Y. An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. [Masters Thesis]. University of Hong Kong; 2011. Available from: Chan, Y. [陳溢恩]. (2011). An investigation of the action of cytokines on human endometrial and endometriotic colony forming units. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807994 ; http://dx.doi.org/10.5353/th_b4807994 ; http://hdl.handle.net/10722/161589

Université Laval

9. Ribeiro de Vargas, Flavia. Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines.

Degree: 2016, Université Laval

URL: http://hdl.handle.net/20.500.11794/26686

►

Le neutrophile est une cellule caractérisée par sa «plasticité» qui, dans des conditions pathologiques, lui permet d'acquérir des nouvelles fonctions qui sont impliquées dans la… (more)

▼

Le neutrophile est une cellule caractérisée par sa «plasticité» qui, dans des conditions pathologiques, lui permet d'acquérir des nouvelles fonctions qui sont impliquées dans la pathogenèse des maladies inflammatoires chroniques comme dans l’arthrite auto-immune (AAI). Les études suggèrent que le neutrophile peut être impliqué dans la résorption osseuse qui accompagne la maladie. Pour mieux analyser cette possibilité, nous avons mis des neutrophiles en présence d’un cocktail de cytokines présentes dans l’AAI afin d’analyser la modulation des gènes liés à la différenciation en cellule de type ostéoclaste. Les résultats ont démontré que le neutrophile est capable d’acquérir de nouvelles fonctions en présence de cytokines, notamment des fonctions importantes dans l’orchestration du système immunitaire et la réponse inflammatoire. Le neutrophile est également une source de molécules permettant la résolution de l’inflammation, notamment l’élafine, ce qui pourrait être un agent thérapeutique prometteur dans les maladies inflammatoires chroniques comme l’AAI.

The plasticity of neutrophils is a very useful property that in pathological conditions allow these cells to acquire new functions involved in the pathogenesis of chronic inflammatory diseases such as autoimmune arthritis (AIA). Studies suggest that neutrophils may be involved in bone resorption accompanying chronic inflammatory diseases. To better analyze this possibility, we have exposed neutrophils to a cocktail of cytokines present in AIA and studied their transcriptome to decipher the modulation of genes related to the differentiation into an osteoclast cell type. The results demonstrated the ability of neutrophils to acquire new functions when exposed to cytokines, including important functions involved in the orchestration of the immune system and inflammatory response. We also demonstrate that the neutrophil is a source of molecules involved in the resolution of inflammation, especially elafin, which seems to be a promising therapeutic agent in chronic inflammatory diseases and perhaps in AIA.

Advisors/Committee Members: Poubelle, Patrice.

Subjects/Keywords: Neutrophiles; Cytokines; Arthrite

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ribeiro de Vargas, F. (2016). Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/26686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ribeiro de Vargas, Flavia. “Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines.” 2016. Thesis, Université Laval. Accessed February 24, 2020. http://hdl.handle.net/20.500.11794/26686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ribeiro de Vargas, Flavia. “Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines.” 2016. Web. 24 Feb 2020.

Vancouver:

Ribeiro de Vargas F. Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines. [Internet] [Thesis]. Université Laval; 2016. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/20.500.11794/26686.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ribeiro de Vargas F. Reprogrammation des neutrophiles en conditions inflammatoires : étude du transcriptome des neutrophiles reprogrammés en présence de cytokines. [Thesis]. Université Laval; 2016. Available from: http://hdl.handle.net/20.500.11794/26686

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Deakin University

10. Mohd Noor, Suzita. Role of Jak2/Stat5/Cis pathway components in zebrafish development.

Degree: School of Medicine, 2011, Deakin University

URL: http://hdl.handle.net/10536/DRO/DU:30036712

► By utilizing the zebrafish as a research model, the function of specific cell signalling pathway components in development was investigated. This revealed new roles for… (more)

Subjects/Keywords: embryology; cytokines; zebrafish

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mohd Noor, S. (2011). Role of Jak2/Stat5/Cis pathway components in zebrafish development. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Thesis, Deakin University. Accessed February 24, 2020. http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Web. 24 Feb 2020.

Vancouver:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Internet] [Thesis]. Deakin University; 2011. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Thesis]. Deakin University; 2011. Available from: http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

11. Wilkinson, Amanda. Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/40599

► Maternal HIV infection during pregnancy is associated with increased risk of adverse obstetric and infant outcomes, and understanding the factors responsible for this association is… (more)

▼ Maternal HIV infection during pregnancy is associated with increased risk of adverse obstetric and infant outcomes, and understanding the factors responsible for this association is essential for designing effective interventions. Maternal malnutrition is an important determinant of maternal and infant health for both HIV-infected and - uninfected women. However, the relative contributions of food insecurity and infectioninduced changes in appetite and body weight regulation that alone, or in combination, contribute to malnutrition among HIV-infected women is largely unknown. The goal of this research was to advance the understanding of the underlying mechanisms by which maternal HIV infection influences pregnancy and early childhood outcomes. A prospective cohort of 114 pregnant women of mixed HIV-status was established in northwestern Tanzania. Pregnant women were followed through delivery and mother-infant dyads participated in follow-up visits at 1, 2, 3 and 6 months postpartum. This study had three main research aims: (1) to investigate maternal HIV and maternal anthropometric indicators as potential determinants of infant outcomes, (2) to characterize maternal and umbilical cord blood cytokine profiles and relate these to infant outcomes, and (3) to evaluate maternal cachexia characteristics during pregnancy. Maternal HIV status and maternal gestational mid-upper arm circumference (MUAC) were compared to infant anthropometric measurements from birth to six months of age. Infants born to HIV-infected mothers weighed, on average, 235 g less and were 1 cm shorter at birth compared to those born to HIV-uninfected mothers. Among mothers of mixed HIV-status, infants born to women with low MUAC had lower birth length and head circumference than infants born to women with higher MUAC. Maternal HIVinfection and low MUAC were also associated with poorer infant growth indicators through six months postpartum. Examination of cytokine profiles during pregnancy in this cohort revealed that HIV-infected pregnant women experienced elevated tumor necrosis factor (TNF)-[alpha]inflammation compared to HIV-uninfected pregnant women. On average, TNF-[alpha] concentrations were 14% higher among HIV-infected pregnant women. Among HIVinfected women, longer duration of antiretroviral therapy and more aggressive antiretroviral therapy were associated with more favorable cytokine profiles. Umbilical cord blood cytokine concentrations were used as a proxy for intrauterine exposure. Elevated umbilical cord blood cytokine concentrations were associated with lower infant birth weight and length. Pregnant women were also evaluated based on biopsychosocial characteristics related to cachexia. Based on a modified cachexia scoring system, HIV-infected and HIV-uninfected pregnant women had comparable cachexia symptoms. Evidence of increased gestational cachexia was associated with less favorable infant birth anthropometrics; and, among HIV-infected mothers, cachexia score was associated with lower infant weight-for-age and length-for-age… Advisors/Committee Members: Lujan,Marla E. (committeeMember), Booth,James (committeeMember), Brannon,Patsy Marie (committeeMember).

Subjects/Keywords: HIV; Pregnancy; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wilkinson, A. (2015). Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/40599

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wilkinson, Amanda. “Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants .” 2015. Thesis, Cornell University. Accessed February 24, 2020. http://hdl.handle.net/1813/40599.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wilkinson, Amanda. “Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants .” 2015. Web. 24 Feb 2020.

Vancouver:

Wilkinson A. Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants . [Internet] [Thesis]. Cornell University; 2015. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/1813/40599.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wilkinson A. Maternal Determinants Of Fetal Growth, Pregnancy Outcomes, And Early Childhood Growth And Development Among Hiv-Exposed And Hiv-Unexposed Infants . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40599

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

12. McGuire, Helen Marie. The role of interleukin-21 in type-1 diabetes.

Degree: Biotechnology & Biomolecular Sciences, 2010, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/50258 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9136/SOURCE02?view=true

► Cytokines are an essential component of both normal and aberrant immune responses, such as in autoimmune disease. Interleukin (IL)-21 is a member of the common… (more)

Subjects/Keywords: Immunology; Diabetes; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McGuire, H. M. (2010). The role of interleukin-21 in type-1 diabetes. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/50258 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9136/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

McGuire, Helen Marie. “The role of interleukin-21 in type-1 diabetes.” 2010. Doctoral Dissertation, University of New South Wales. Accessed February 24, 2020. http://handle.unsw.edu.au/1959.4/50258 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9136/SOURCE02?view=true.

MLA Handbook (7^th Edition):

McGuire, Helen Marie. “The role of interleukin-21 in type-1 diabetes.” 2010. Web. 24 Feb 2020.

Vancouver:

McGuire HM. The role of interleukin-21 in type-1 diabetes. [Internet] [Doctoral dissertation]. University of New South Wales; 2010. [cited 2020 Feb 24]. Available from: http://handle.unsw.edu.au/1959.4/50258 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9136/SOURCE02?view=true.

Council of Science Editors:

McGuire HM. The role of interleukin-21 in type-1 diabetes. [Doctoral Dissertation]. University of New South Wales; 2010. Available from: http://handle.unsw.edu.au/1959.4/50258 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9136/SOURCE02?view=true

University of Ontario Institute of Technology

13. Shastri, Padmaja. Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions.

Degree: 2015, University of Ontario Institute of Technology

URL: http://hdl.handle.net/10155/532

► Sex-based differences in immune parameters are well recognized, and recent evidence suggests differences in microbiota composition between sexes, necessitating assessment of effects of dietary fermentable… (more)

▼ Sex-based differences in immune parameters are well recognized, and recent evidence suggests differences in microbiota composition between sexes, necessitating assessment of effects of dietary fermentable material (DFM) in both males and females. Effects of consumption of DFM of differing composition (Wheat Bran (WB), Oat Bran (OB), Resistant Starch (RS), Fructooligosaccharides (FOS)) on the immune system were compared under resting and immunized conditions between sexes. The gut microbiota composition and short chain fatty acid profiles differed between rats fed different DFM and also differed between sexes following FOS consumption. Immune parameters were analyzed for the effect of diet, differences between sexes or interactions (diet x sex), to detect the effect of DFM on male and female rats. The kinetics of primary (Day 0, 5, 10, 15) and secondary antibody responses (Day 21) were measured following immunization with a T cell-dependent antigen, Keyhole Limpet Hemocyanin (KLH). Under resting conditions, male rats had more FoxP3+ Treg cells in the mesenteric lymph node (MLN) and spleen than did females. Levels of the regulatory cytokine TGF-??1 also increased in the MLN in a DFM-dependent manner. In contrast, percentages of MLN and splenic CD68+ macrophages and levels of gut tissue IL-10 were higher in females than males. These results suggest immune regulation at the mucosal level is controlled in a differential manner between sexes and was affected by DFM intake only in males. At the systemic level, DFM intake delayed the primary anti-KLH IgG antibody response kinetics in male (control>>WB>OB>RS>FOS) and female (control> FOS>WB>RS>OB) rats. The secondary anti-KLH IgG response in control-fed males was 10-fold higher than in control-fed females. DFM intake in males significantly decreased anti-KLH IgG levels relative to control-fed males. Collectively, these findings demonstrate DFM consumption in males potentially induces systemic immune regulation leading to a delayed response to immune challenge, an effect that was not observed in females. Advisors/Committee Members: Green-Johnson, Julia.

Subjects/Keywords: Cytokines; Immunoglobulins; Microbiota; Sex; Fibre

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shastri, P. (2015). Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions. (Thesis). University of Ontario Institute of Technology. Retrieved from http://hdl.handle.net/10155/532

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shastri, Padmaja. “Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions.” 2015. Thesis, University of Ontario Institute of Technology. Accessed February 24, 2020. http://hdl.handle.net/10155/532.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shastri, Padmaja. “Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions.” 2015. Web. 24 Feb 2020.

Vancouver:

Shastri P. Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions. [Internet] [Thesis]. University of Ontario Institute of Technology; 2015. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/10155/532.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shastri P. Effects of dietary fermentable material on innate and adaptive immune measures in male and female rats under resting and immunized conditions. [Thesis]. University of Ontario Institute of Technology; 2015. Available from: http://hdl.handle.net/10155/532

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ontario Institute of Technology

14. McCarville, Justin. Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells.

Degree: 2012, University of Ontario Institute of Technology

URL: http://hdl.handle.net/10155/275

► Probiotics and prebiotics are emerging household terms, whose claimed health benefits share commonality. Their attributed health benefits include the production or induction of short chain… (more)

▼ Probiotics and prebiotics are emerging household terms, whose claimed health benefits share commonality. Their attributed health benefits include the production or induction of short chain fatty acids, maintaining bowel function, building colonization resistance (against pathogens) and treating antibiotic-associated diarrhea as well as colitis. Although both probiotic and prebiotic effects on immune system have been studied, the mechanisms of their activity are still not clearly defined and the conclusions drawn are elusive. While probiotics can act to influence the host at the cellular level, prebiotics, by definition, exert their effects indirectly through their impact on gut microbes. One purpose of this study was to investigate effects of Lactobacillus rhamnosus R0011 on innate immune parameters at the intestinal epithelial cell level, examining effects on both human and rat IEC. A second purpose was to define the effects of a range of prebiotic dietary fibres on the immune system at the mucosal and systemic level, using Biobreeding rats. L. rhamnosus demonstrated the ability to decrease proinflammatory cytokine and Toll-like receptor agonist-induced IL-8 and CINC-1 production from human and rat IEC, respectively. The timing of L. rhamnosus R0011 addition to HT-29 IEC, relative to proinflammatory challenge, influenced its ability to decrease IL-8 production. L. rhamnosus was more effective at decreasing production of IL-8 from human IEC when they were pre-incubated with this bacterium and subsequently challenged with proinflammatory stimuli. Certain effects of L. rhamnosus R011 were also observed in the absence of proinflammatory stimuli. Viable L. rhamnosus induced TNF-?? production from rat IEC and heat-killed L. rhamnosus decreased constitutive TGF-?? production from rat IEC and induced IL-8 or CINC-1 production from human and rat IEC, respectively. In Biobreeding rats, we demonstrated that oat dietary fibre significantly alters active TGF-??, CINC-1 and IL-6 levels in the colon in comparison to AIN-93G-fed rats. Wheat dietary fibre induced changes in active TGF-??, CINC-1 and IL-4 levels in the ileum in comparison to resistant starch-fed rats. Lastly, resistant starch exerted effects in the mesenteric lymph node, where changes in active TGF-?? were observed in rats in comparison to AIN-93G-fed rats. Oat bran, wheat bran and resistant starch had no effects on cytokine levels in the serum or spleen of rats. Fructooligosaccharide-fed rats had a significant increase in active TGF-?? levels in the colon and a significant decrease in active TGF-?? levels in the spleen. Overall this suggests a FOS supplemented diet has both mucosal and systemic effects in rats, while wheat, oat and resistant starch supplemented diets had effects focused at the different locations at the mucosal level. These results illustrate differences in the ability of different dietary fibres to target immune parameters in specific mucosal tissues along the gastrointestinal tract and differential ability to exert systemic effects.… Advisors/Committee Members: Green-Johnson, Julia.

Subjects/Keywords: Probiotics; Prebiotics; Microflora; GALT; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McCarville, J. (2012). Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells. (Thesis). University of Ontario Institute of Technology. Retrieved from http://hdl.handle.net/10155/275

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCarville, Justin. “Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells.” 2012. Thesis, University of Ontario Institute of Technology. Accessed February 24, 2020. http://hdl.handle.net/10155/275.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCarville, Justin. “Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells.” 2012. Web. 24 Feb 2020.

Vancouver:

McCarville J. Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells. [Internet] [Thesis]. University of Ontario Institute of Technology; 2012. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/10155/275.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCarville J. Effects of prebiotic fibre diets on rat mucosal intestinal and systemic immunity and in vitro mechanistic analysis of anti-inflammatory effects of lactobacillus strains on rat and human intestinal epithelial cells. [Thesis]. University of Ontario Institute of Technology; 2012. Available from: http://hdl.handle.net/10155/275

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

15. Allen, Charlotte Annette. Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2124

► The replication of equine infectious anemia virus (EIAV) in macrophages not only leads to cell death, but also to the induction of a variety of… (more)

Subjects/Keywords: Cytokines; Macrophages; Horse; QPCR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Allen, C. A. (2009). Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2124

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Allen, Charlotte Annette. “Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction.” 2009. Thesis, Texas A&M University. Accessed February 24, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-2124.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Allen, Charlotte Annette. “Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction.” 2009. Web. 24 Feb 2020.

Vancouver:

Allen CA. Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2124.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Allen CA. Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2124

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

16. Gao, Ying. Role of cytokines in the regulation of cell junction dynamics in the testis.

Degree: PhD, 2013, University of Hong Kong

URL: Gao, Y. [高莹]. (2013). Role of cytokines in the regulation of cell junction dynamics in the testis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5066203 ; http://dx.doi.org/10.5353/th_b5066203 ; http://hdl.handle.net/10722/200166

► During spermatogenesis, developing germ cells must migrate across the blood-testis barrier (BTB) and enter the adluminal compartment for further development. Throughout this process, extensive junction… (more)

▼ During spermatogenesis, developing germ cells must migrate across the blood-testis barrier (BTB) and enter the adluminal compartment for further development. Throughout this process, extensive junction restructuring occurs at Sertoli-Sertoli and Sertoli-germ cell interfaces. Cytokines are known to play crucial roles in regulating testicular cell junction dynamics at different regulatory levels. However, the mechanism of cytokine-mediated regulation on newly identified junction molecules remains unclear. In this dissertation, the molecular mechanisms on how cytokines regulate the junction proteins of immunoglobulin superfamily (IgSF) including coxsackievirus and adenovirus receptor (CAR), nectin-like molecule-2 (Necl-2) and Necl-4 in testicular cells were studied. CAR is expressed on Sertoli and germ cells. It mediates both homophilic and heterophilic interaction for Sertoli-germ cell adhesion. It was found that combined treatment of interferon-γ (IFN-γ) and tumor necrosis factor α (TNFα) reduced CAR mRNA and protein levels, and caused the disappearance of CAR from germ cell interface. IFN-γ+TNFα promoted CAR protein degradation via ubiquitin-proteasome pathway. In addition, IFN-γ+TNFα reduced CAR mRNA through regulating the binding of NF-κB subunits and SP/KLF proteins to CAR promoter. Collectively, these results demonstrated for the first time the potential mechanism utilized by IFN-γ+TNFα to exert their effects during testicular inflammation. Necl-2 is exclusively expressed by spermatogenic cells in the testis. In this study, it was demonstrated that transforming growth factor-β1 (TGF-β1) down-regulated Necl-2 mRNA and protein levels, and caused the disappearance of Necl-2 from cell surface. Using inhibitors and shRNAs, it was found that TGF-β1 induced Necl-2 protein degradation through clathrin-dependent endocytosis. Endocytosis assay further confirmed that TGF-β1 accelerated the internalization of Necl-2 to cytosol. Moreover, TGF-β1 repressed Necl-2 gene transcription in Smad-dependent manner. Taken together, these results unraveled the mechanism of how TGF-β1 regulates Necl-2 expression to achieve timely junction restructuring during spermatogenesis. Necl-4 has been detected in Sertoli cells, but little is known about its regulation in the testis. It was found that TNFα down-regulated Necl-4 mRNA and protein levels. Inhibitor studies suggested that both caveolin-dependent endocytosis and ubiquitin-proteasome pathway were involved in TNFα-induced Necl-4 protein degradation. Co-immunoprecipitation indicated that Necl-4 was physically associated with Par3, Par6, aPKC and Cdc42 which are the major components of polarity complex in mouse testis. Further study was shown that TNFα reduced the expression of Par3, and altered the binding between Necl-4 and Par complex in Sertoli cells. These results suggested that Necl-4-mediated cell adhesion could be disrupted by TNFα via reducing its expression and altering its interaction with Par complex. Studies reported herein suggest that junction proteins of the IgSF…

Subjects/Keywords: Cytokines; Cell junctions; Testis - Cytology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, Y. (2013). Role of cytokines in the regulation of cell junction dynamics in the testis. (Doctoral Dissertation). University of Hong Kong. Retrieved from Gao, Y. [高莹]. (2013). Role of cytokines in the regulation of cell junction dynamics in the testis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5066203 ; http://dx.doi.org/10.5353/th_b5066203 ; http://hdl.handle.net/10722/200166

Chicago Manual of Style (16^th Edition):

Gao, Ying. “Role of cytokines in the regulation of cell junction dynamics in the testis.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed February 24, 2020. Gao, Y. [高莹]. (2013). Role of cytokines in the regulation of cell junction dynamics in the testis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5066203 ; http://dx.doi.org/10.5353/th_b5066203 ; http://hdl.handle.net/10722/200166.

MLA Handbook (7^th Edition):

Gao, Ying. “Role of cytokines in the regulation of cell junction dynamics in the testis.” 2013. Web. 24 Feb 2020.

Vancouver:

Gao Y. Role of cytokines in the regulation of cell junction dynamics in the testis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2020 Feb 24]. Available from: Gao, Y. [高莹]. (2013). Role of cytokines in the regulation of cell junction dynamics in the testis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5066203 ; http://dx.doi.org/10.5353/th_b5066203 ; http://hdl.handle.net/10722/200166.

Council of Science Editors:

Gao Y. Role of cytokines in the regulation of cell junction dynamics in the testis. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Gao, Y. [高莹]. (2013). Role of cytokines in the regulation of cell junction dynamics in the testis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5066203 ; http://dx.doi.org/10.5353/th_b5066203 ; http://hdl.handle.net/10722/200166

University of Hong Kong

17. 黃沛珊; Wong, Bauhinia. Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages.

Degree: Master of Medical Sciences, 2014, University of Hong Kong

URL: Wong, B. [黃沛珊]. (2014). Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303984 ; http://dx.doi.org/10.5353/th_b5303984 ; http://hdl.handle.net/10722/206489

►

Chronic obstructive pulmonary disease (COPD) is a progressive disease and characterized by persistent airflow limitation. Pathophysiologically it involves many components, including oxidative stress and inflammation… (more)

▼

Chronic obstructive pulmonary disease (COPD) is a progressive disease and characterized by persistent airflow limitation. Pathophysiologically it involves many components, including oxidative stress and inflammation of the airways and lungs. Although the primary cause of COPD is smoking, acute exacerbation due to infections can accelerate disease progression, which is a significant cause of morbidity, mortality and burden on healthcare costs. One-half of all acute exacerbations of COPD are associated with bacterial infection, with non-typeable Hemophilus influenzae being the most common pathogen. Staphylococcus pneumonia, one of the most common Gram-positive bacterial pathogens, is also involved in airway infections, either primary or subsequent to viral diseases. To COPD patients the common respiratory pathogens include Gram-negative and Gram-positive bacterial species. Lipopolysaccharide (LPS), a major component of the outer membrane of all Gram-negative bacteria, which is the predominant inducer of inflammatory responses, has been widely studied. On the other hand, less is known about Gram-positive bacteria, which do not contain LPS but express lipoteichoic acid (LTA) as an important proinflammatory constituent in their cell wall. Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter that plays an important role in regulating pulmonary function and pathogenesis of inflammation. In this study, we hypothesize that the serotoninergic system may be involved in LPS- and LTA-induced inflammation in COPD. Since monocyte recruitment to lung is a key step in COPD, this study aims to investigate the effects of LPS or LTA alone and in combination of 5-HT pretreatment on the release of pro-inflammatory cytokines in undifferentiated (i.e. monocytes) and differentiated THP-1 cells (i.e. macrophages). LPS, LTA or 5-HT alone induced the release of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in both monocytes and macrophages. Our findings also showed that 5-HT pretreatment suppressed the LPS-induced IL-8 and MCP-1 release, suggesting that 5-HT might act as an anti-inflammatory mediator. On the other hand, 5-HT pretreatment enhanced the LTA-induced IL-8 and MCP-1 release, indicating that 5-HT might also act as a pro-inflammatory mediator. These results demonstrate that 5-HT may be involved in the differential modulation of inflammatory processes during Gram-negative or Gram-positive infections in COPD.

published_or_final_version

Medicine

Master

Master of Medical Sciences

Subjects/Keywords: Macrophages; Cytokines; Monocytes; Serotonin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

黃沛珊; Wong, B. (2014). Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. (Masters Thesis). University of Hong Kong. Retrieved from Wong, B. [黃沛珊]. (2014). Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303984 ; http://dx.doi.org/10.5353/th_b5303984 ; http://hdl.handle.net/10722/206489

Chicago Manual of Style (16^th Edition):

黃沛珊; Wong, Bauhinia. “Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages.” 2014. Masters Thesis, University of Hong Kong. Accessed February 24, 2020. Wong, B. [黃沛珊]. (2014). Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303984 ; http://dx.doi.org/10.5353/th_b5303984 ; http://hdl.handle.net/10722/206489.

MLA Handbook (7^th Edition):

黃沛珊; Wong, Bauhinia. “Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages.” 2014. Web. 24 Feb 2020.

Vancouver:

黃沛珊; Wong B. Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. [Internet] [Masters thesis]. University of Hong Kong; 2014. [cited 2020 Feb 24]. Available from: Wong, B. [黃沛珊]. (2014). Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303984 ; http://dx.doi.org/10.5353/th_b5303984 ; http://hdl.handle.net/10722/206489.

Council of Science Editors:

黃沛珊; Wong B. Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. [Masters Thesis]. University of Hong Kong; 2014. Available from: Wong, B. [黃沛珊]. (2014). Effects of serotonin on LPS- or LTA- stimulated cytokine release in monocytes and macrophages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5303984 ; http://dx.doi.org/10.5353/th_b5303984 ; http://hdl.handle.net/10722/206489

18. Sonia Devi Lourembam. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.

Degree: Biotechnology, 2011, Tezpur University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/9014

►

Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread… (more)

▼

Malaria is a major health problem with the disease burden mostly borne by economically productive ages. Recent studies indicate the disease is becoming more widespread in south east Asia with 10 of the 11 countries endemic for malaria and with 1.2 billion people at risk of exposure to the disease. India reported the highest malaria confirmed (1563344) and death (1133) cases in 2009 from this region (WHO). The north-eastern region of India (population 28.5 million) is highly endemic for malaria and has been declared as a high risk zone by National Anti Malaria Program. Assam contributes to 64.7% of the malaria positive cases in the north-eastern region of which P. falciparum accounts for 58-75% of the cases and the remainder are due to Plasmodium vivax. Though people living in endemic area develop immunity, which can be either anti disease or anti infection immunity, the factors delineating them are not clear. It is thus felt that an in depth understanding of the immune response leading to anti infection or to anti disease responses in population genetic studies and elucidation of parasite diversity is required for success of malaria control programs. The present study was designed against this perspective with the following objectives: 1) To study the clone multiplicity of the parasite genotypes existing in the study area 2) To elucidate the protective humoral immune response. 3) To elucidate the protective cell mediated immune response. The study was conducted at Guabari village of Baksa district and Kondoli Tea Estate (KTE) of Nagaon district which are mesoendemic for malaria. The two sites differ in population demography and accessibility to health care. Plasmodium falciparum diversity was assessed by typing polymorphic block 2 region of Merozoite Surface Protein 1(MSP-1) gene using primary as well as nested PCR cycles. A high degree of polymorphism in isolates from the two study sites was seen with 33 alleles of MSP-1 seen in Guabari village and 18 alleles at KTE.

References p.143-164, Abstract given chapter wise

Advisors/Committee Members: Baruah, S.

Subjects/Keywords: Biology; Plasmodium falciparum; Malaria; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lourembam, S. D. (2011). Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. (Thesis). Tezpur University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/9014

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.” 2011. Thesis, Tezpur University. Accessed February 24, 2020. http://shodhganga.inflibnet.ac.in/handle/10603/9014.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lourembam, Sonia Devi. “Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -.” 2011. Web. 24 Feb 2020.

Vancouver:

Lourembam SD. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. [Internet] [Thesis]. Tezpur University; 2011. [cited 2020 Feb 24]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lourembam SD. Protective immune response in plasmodium falciparum malaria: a molecular immunoepidemiological study; -. [Thesis]. Tezpur University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9014

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

19. La Manna, Vincenzo. Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw.

Degree: 2008, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25497 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499626

► The study investigated healthy claw samples from cross-bred heifers aged around 20 months. Histological studies adopted a novel sectioning approach along planes parallel to the… (more)

Subjects/Keywords: 636.089; Lameness in cattle : Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

La Manna, V. (2008). Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25497 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499626

Chicago Manual of Style (16^th Edition):

La Manna, Vincenzo. “Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw.” 2008. Doctoral Dissertation, University of Aberdeen. Accessed February 24, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25497 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499626.

MLA Handbook (7^th Edition):

La Manna, Vincenzo. “Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw.” 2008. Web. 24 Feb 2020.

Vancouver:

La Manna V. Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw. [Internet] [Doctoral dissertation]. University of Aberdeen; 2008. [cited 2020 Feb 24]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25497 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499626.

Council of Science Editors:

La Manna V. Microanatomy, structural macromolecules and growth factors expression in the laminar region of the bovine claw. [Doctoral Dissertation]. University of Aberdeen; 2008. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25497 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499626

University of Aberdeen

20. Holt, Amy. Discovery and characterisation of cytokines involved in T-helper cell responsed in teleosts.

Degree: 2011, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167820 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554217

► Much of the research that has been done on the fish immune system has focussed on innate immunity. Very little is known about the adaptive… (more)

Subjects/Keywords: 616.079; Osteichthyes : Helper Cells : Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Holt, A. (2011). Discovery and characterisation of cytokines involved in T-helper cell responsed in teleosts. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167820 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554217

Chicago Manual of Style (16^th Edition):

Holt, Amy. “Discovery and characterisation of cytokines involved in T-helper cell responsed in teleosts.” 2011. Doctoral Dissertation, University of Aberdeen. Accessed February 24, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167820 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554217.

MLA Handbook (7^th Edition):

Holt, Amy. “Discovery and characterisation of cytokines involved in T-helper cell responsed in teleosts.” 2011. Web. 24 Feb 2020.

Vancouver:

Holt A. Discovery and characterisation of cytokines involved in T-helper cell responsed in teleosts. [Internet] [Doctoral dissertation]. University of Aberdeen; 2011. [cited 2020 Feb 24]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167820 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554217.

Council of Science Editors:

Holt A. Discovery and characterisation of cytokines involved in T-helper cell responsed in teleosts. [Doctoral Dissertation]. University of Aberdeen; 2011. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167820 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554217

21. Ρεγκλή - Γκούμα, Αρετή. Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα.

Degree: 2004, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/13745

Subjects/Keywords: Κυτταροκίνες; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ρεγκλή - Γκούμα, . �. (2004). Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/13745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ρεγκλή - Γκούμα, Αρετή. “Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα.” 2004. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed February 24, 2020. http://hdl.handle.net/10442/hedi/13745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ρεγκλή - Γκούμα, Αρετή. “Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα.” 2004. Web. 24 Feb 2020.

Vancouver:

Ρεγκλή - Γκούμα �. Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2004. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/10442/hedi/13745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ρεγκλή - Γκούμα �. Επίπεδα κυτταροκινών σε ασθενείς με αιματολογικά νοσήματα. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2004. Available from: http://hdl.handle.net/10442/hedi/13745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Wright State University

22. Mullins, Jeremi Stevan. Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei.

Degree: MS, Biological Sciences, 2008, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687

► Sarcoptes scabiei infests its host through burrowing into the stratum corneum layer of skin. Keratinocytes and fibroblasts responding to the burrowing mite(s) and/or mite products… (more)

Subjects/Keywords: Immunology; Cytokines; Scabies; Keratinocytes; Fibroblasts

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mullins, J. S. (2008). Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei. (Masters Thesis). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687

Chicago Manual of Style (16^th Edition):

Mullins, Jeremi Stevan. “Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei.” 2008. Masters Thesis, Wright State University. Accessed February 24, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687.

MLA Handbook (7^th Edition):

Mullins, Jeremi Stevan. “Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei.” 2008. Web. 24 Feb 2020.

Vancouver:

Mullins JS. Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei. [Internet] [Masters thesis]. Wright State University; 2008. [cited 2020 Feb 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687.

Council of Science Editors:

Mullins JS. Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei. [Masters Thesis]. Wright State University; 2008. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687

Wright State University

23. Caraway, Jessie. Sensitization of behavioral response to maternal separation: persistence of the effect and role of proinflammatory activity.

Degree: MS, Anatomy, 2010, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1279025047

► Maternal separation in guinea pigs produces a biphasic response consisting of an active behavior phase followed by a phase of passive behavior (crouched stance, piloerection,… (more)

Subjects/Keywords: Psychobiology; Cytokines; sensitization; maternal separation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Caraway, J. (2010). Sensitization of behavioral response to maternal separation: persistence of the effect and role of proinflammatory activity. (Masters Thesis). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1279025047

Chicago Manual of Style (16^th Edition):

Caraway, Jessie. “Sensitization of behavioral response to maternal separation: persistence of the effect and role of proinflammatory activity.” 2010. Masters Thesis, Wright State University. Accessed February 24, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1279025047.

MLA Handbook (7^th Edition):

Caraway, Jessie. “Sensitization of behavioral response to maternal separation: persistence of the effect and role of proinflammatory activity.” 2010. Web. 24 Feb 2020.

Vancouver:

Caraway J. Sensitization of behavioral response to maternal separation: persistence of the effect and role of proinflammatory activity. [Internet] [Masters thesis]. Wright State University; 2010. [cited 2020 Feb 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1279025047.

Council of Science Editors:

Caraway J. Sensitization of behavioral response to maternal separation: persistence of the effect and role of proinflammatory activity. [Masters Thesis]. Wright State University; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1279025047

Universiteit Utrecht

24. Vorage, M.S.C.E. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.

Degree: 2011, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/213023

► Fibromyalgia (FM) is a common disorder of unclear aetiology, characterized by chronic widespread pain and painful tender points (body pressure points). Several researchers suggest that… (more)

Subjects/Keywords: Fibromyalgia; cytokines; oxidative stress

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vorage, M. S. C. E. (2011). What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/213023

Chicago Manual of Style (16^th Edition):

Vorage, M S C E. “What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.” 2011. Masters Thesis, Universiteit Utrecht. Accessed February 24, 2020. http://dspace.library.uu.nl:8080/handle/1874/213023.

MLA Handbook (7^th Edition):

Vorage, M S C E. “What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?.” 2011. Web. 24 Feb 2020.

Vancouver:

Vorage MSCE. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. [Internet] [Masters thesis]. Universiteit Utrecht; 2011. [cited 2020 Feb 24]. Available from: http://dspace.library.uu.nl:8080/handle/1874/213023.

Council of Science Editors:

Vorage MSCE. What are the roles of oxidative stress and pro-inflammatory cytokines in fibromyalgia?. [Masters Thesis]. Universiteit Utrecht; 2011. Available from: http://dspace.library.uu.nl:8080/handle/1874/213023

University of Southern California

25. Tam, Yvonne. Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics.

Degree: MS, Cranio-Facial Biology, 2012, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1298

► Background: Some investigators have detected edema, swelling and histological changes in the soft tissue under pontics, while others have demonstrated that exceptional plaque control can… (more)

▼ Background: Some investigators have detected edema, swelling and histological changes in the soft tissue under pontics, while others have demonstrated that exceptional plaque control can lead to healthy soft tissue at pontic sites. A new pontic design by Kim et al. (2009) attempts to address the issue of esthetics and oral hygiene by creating a ridge lap pontic with circumferential pressure, and was hypothesized to be associated with favorable microbiological and inflammatory profiles. ❧ Objective: The aim of this study was to explore the microbiological and cytokine/chemokine profiles of implants as well as the “pressure pontic” site in implantsupported fixed partial dentures and contrast them to that of healthy teeth. ❧ Materials and Methods: Gingival crevicular fluid was sampled from 6 patients, each with an implant-supported fixed prosthesis and a healthy tooth. One implant, the adjacent pontic site, and the contralateral tooth from the implant sampled were sampled whenever possible with Periopaper strips and subgingival plaque was collected with paper points. Both were placed separately into the peri-implant sulcus, periodontal sulcus as well as the pontic-mucosa interface on the mesio- and disto-lingual surfaces. Microbial samples were analyzed for periodontopathic bacteria via selective anaerobic culture techniques. Proinflammatory cytokines were quantified by flow cytometry analysis of GCF with two separate kits. The first kit tested for IL-8, IL-6, IL-1β, TNF-α, and IL-10 while the second one detected the concentration of IL-8, IP-10, RANTES, MCP-1, and MIG. ❧ Results: The number of sites along with the cultivable levels of periodontopathic bacteria detected in pontic sites, implant sulci, and periodontal sulci were similar. There were no significant differences detected in cytokines and chemokines due to a wide range of values and limited number of samples. The results of the chemokine values for patients 5 and 6, who were sampled after one month, appeared higher than those of patients 1-4, who were sampled one year after implant restoration. ❧ Conclusion: The ridge lap pontic with circumferential pressure merits further evaluation. The microbiological profile of the pontic site was comparable to that of other pontic research designs observed by other researchers (Wang et al. 1998). The pontic area also provided esthetic results, a lack of clinical inflammation, as well as a decrease in immune response over time. Advisors/Committee Members: Zadeh, Homah (Committee Chair), Rich, Sandra (Committee Member), Navazesh, Mahvash (Committee Member).

Subjects/Keywords: cytokines; chemokines; implants; pontics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tam, Y. (2012). Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1298

Chicago Manual of Style (16^th Edition):

Tam, Yvonne. “Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics.” 2012. Masters Thesis, University of Southern California. Accessed February 24, 2020. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1298.

MLA Handbook (7^th Edition):

Tam, Yvonne. “Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics.” 2012. Web. 24 Feb 2020.

Vancouver:

Tam Y. Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics. [Internet] [Masters thesis]. University of Southern California; 2012. [cited 2020 Feb 24]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1298.

Council of Science Editors:

Tam Y. Characterization of cytokine/chemokine and microbiology profiles of peri-implant sulci and implant-supported ridge lap pontics. [Masters Thesis]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/93541/rec/1298

University of Iceland

26. Una Björk Jóhannsdóttir 1980. Activity of Atlantic cod trypsin towards cytokines and other proteins.

Degree: 2009, University of Iceland

URL: http://hdl.handle.net/1946/2511

►

Fyrri rannsóknir sýndu að þorskatrypsín er virkast 15 samanburðarensíma gagnvart cytokínum er gegna hlutverki í ónæmissvörun og bólgumyndun. Þrjú náttúruleg afbrigði trypsíns (trypsín I, II… (more)

Subjects/Keywords: Matvælafræði; Protein; Cytokines; Cod trypsin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

1980, U. B. J. (2009). Activity of Atlantic cod trypsin towards cytokines and other proteins. (Thesis). University of Iceland. Retrieved from http://hdl.handle.net/1946/2511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

1980, Una Björk Jóhannsdóttir. “Activity of Atlantic cod trypsin towards cytokines and other proteins.” 2009. Thesis, University of Iceland. Accessed February 24, 2020. http://hdl.handle.net/1946/2511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

1980, Una Björk Jóhannsdóttir. “Activity of Atlantic cod trypsin towards cytokines and other proteins.” 2009. Web. 24 Feb 2020.

Vancouver:

1980 UBJ. Activity of Atlantic cod trypsin towards cytokines and other proteins. [Internet] [Thesis]. University of Iceland; 2009. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/1946/2511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

1980 UBJ. Activity of Atlantic cod trypsin towards cytokines and other proteins. [Thesis]. University of Iceland; 2009. Available from: http://hdl.handle.net/1946/2511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

27. Pedley, Renée Marie. The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep.

Degree: MS, 2010, Massey University

URL: http://hdl.handle.net/10179/2682

► To examine gene expression in vivo, total RNA was extracted from articular cartilage and subchondral bone. As limited methodology existed for ovine, RNA extraction was… (more)

Subjects/Keywords: RNA extraction; Cytokines; Collagen

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pedley, R. M. (2010). The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/2682

Chicago Manual of Style (16^th Edition):

Pedley, Renée Marie. “The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep.” 2010. Masters Thesis, Massey University. Accessed February 24, 2020. http://hdl.handle.net/10179/2682.

MLA Handbook (7^th Edition):

Pedley, Renée Marie. “The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep.” 2010. Web. 24 Feb 2020.

Vancouver:

Pedley RM. The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep. [Internet] [Masters thesis]. Massey University; 2010. [cited 2020 Feb 24]. Available from: http://hdl.handle.net/10179/2682.

Council of Science Editors:

Pedley RM. The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep. [Masters Thesis]. Massey University; 2010. Available from: http://hdl.handle.net/10179/2682

28. Μπούμπαλη, Σταυρούλα. Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα.

Degree: 2009, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3419

► Το ασβέστιο αποτελεί ένα σημαντικό δεύτερο μήνυμα που ρυθμίζει την ανοσολογική απόκριση. Η αύξηση του ενδοκυττάριου ασβεστίου αυξάνει την παραγωγή της IL-10 στα Τ-λεμφοκύτταρα, αλλά… (more)

▼ Το ασβέστιο αποτελεί ένα σημαντικό δεύτερο μήνυμα που ρυθμίζει την ανοσολογική απόκριση. Η αύξηση του ενδοκυττάριου ασβεστίου αυξάνει την παραγωγή της IL-10 στα Τ-λεμφοκύτταρα, αλλά δεν είναι σαφές ποια ενδοκυττάρια μόρια εμπλέκονται σε αυτή τη ρύθμιση. Σκοπός της μελέτης είναι η αποσαφήνιση της συμμετοχής του ασβεστίου στην παραγωγή της IL-10 από Τ-λεμφοκύτταρα και η εντόπιση των πιθανών ενδοκυττάριων στόχων. Ο ρόλος των ενζύμων κλειδιών στην οδό ενεργοποίησης μέσω ασβεστίου καλσινευρίνης και CaM Kinase II μελετήθηκε με χρήση είτε ειδικών αναστολέων, κυκλοσπορίνης και ΚΝ-62 αντίστοιχα, είτε με πλασμίδια που κωδικοποιούν τις καταλυτικές υπομονάδες των ενζύμων καλσινευρίνη και CaM Kinase II. Ανθρώπινα Τ-λεμφοκύτταρα περιφερικού αίματος διεγέρθηκαν με anti-CD3/anti-CD28 (διέγερση μέσω TCR), ή Ionomycin/PMA (ενδοκυττάρια στόχευση Ca++ και PKC) παρουσία ή απουσία των ειδικών αναστολέων. Το πρωτεϊνικό προϊόν της IL-10 μετρήθηκε με ELISA ενώ η παραγωγή mRNA της IL-10 με Real Time PCR. Η ενεργότητα του υποκινητή της IL-10, IL-2, IL-4 παρουσία ή απουσία των ενεργών ενζύμων ελέγχθηκε με διαμόλυνση των κυττάρων με πλασμίδια που φέρουν τον υποκινητή του γονιδίου (1327 bp) ή τμήματα αυτού (-1010, -500, -310, -235, -135 bp Η δράση των ίδιων ενζύμων στην ενεργότητα των μεταγραφικών παραγόντων MEF2, CREB και NF-κB ελέγχθηκε με ταυτόχρονη έκφρασή τους in vivo με πειράματα διαμολύνσεως με πλασμίδια που ελέγχουν την ενεργότητα της λουσιφεράσης υπό τον έλεγχο τους και in vitro σε πυρηνικά πρωτεϊνικά εκχυλίσματα με πειράματα EMSA. Η επίδραση του μεταγραφικού παράγοντα MEF2 στον υποκινητή της IL-10 έγινε μέσω υπερέκφρασης του in vivo σε πειράματα διαμόλυνσης. Η IL-10 ανιχνεύεται 24 ώρες μετά τη διέγερση των κυττάρων, φτάνει στο μέγιστο στις 48 ώρες και μειώνεται μετά τις 72 ώρες. Παρουσία κυκλοσπορίνης η παραγωγή της IL-10 μειώθηκε κατά 80%-100%, ενώ παρουσία ΚΝ-62 η παραγωγή της IL-10 μειώθηκε κατά 70%-90%, σε όλα τα χρονικά διαστήματα στα οποία μελετήθηκε. Τα ποσοστά αναστoλής ήταν ίδια και κατά τους δύο τρόπους διέγερσης. Οταν τα κύτταρα διεγέρθηκαν μέσω TCR και του συνδιεγερτικού μορίου CD28, η αναστολή των παραπάνω ενζύμων δεν επηρεάζει την συσσώρευση του mRNA της IL-10, υποδηλώνοντας ότι η ρύθμιση γίνεται σε μεταγενέστερα της μεταγραφής στάδια, επηρεάζοντας πιθανά τη σταθερότητα του mRNA ή το ρυθμό μετάφρασής του. Όταν ο τρόπος διέγερσης των κυττάρων παρέκαμπτε τον TCR τότε η ελάττωση της παραγωγής της IL-10 αντανακλούσε και σε επίπεδο mRNA. Η ενεργοποίηση του υποκινητή της IL-10 που παρατηρείται μετά από διέγερση με ΙON/PMA και CD3/CD28 αυξήθηκε κατά 5 και 3 φορές αντίστοιχα σε Τ λεμφοκύτταρα τα οποία είχαν διαμολυνθεί με τις ενεργές μορφές των ενζύμων καλσινευρίνη και CaM Kinase II σε σχέση με τα κύτταρα τα οποία είχαν διαμολυνθεί μόνο με το όχημα pSRa. Η δράση της CaM Kinase II είναι εκλεκτική για τον υποκινητή της ΙL-10 εφόσον το ίδιο ένζυμο μειώνει την δραστικότητα του υποκινητή τόσο μιας άλλης Th2 κυτταροκίνης όπως η IL-4 όπως επίσης και της IL-2, ενώ η καλσινευρίνη έχει θετική επίδραση στους υποκινητές και των… Advisors/Committee Members: Παληογιάννη, Φωτεινή, Boumbali, Stavroula, Δημητρακόπουλος, Γεώργιος, Παπαβασιλείου, Αθανάσιος, Αναστασίου, Ευάγγελος, Χρυστοφίδου, Μυρτώ, Ζαρκάδης, Ιωάννης, Κολονίτσου, Φεβρονία.

Subjects/Keywords: Ανοσολογία; Κυτταροκίνες; 571.966; Immunology; Cytokines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Μπούμπαλη, �. (2009). Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα. (Doctoral Dissertation). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3419

Chicago Manual of Style (16^th Edition):

Μπούμπαλη, Σταυρούλα. “Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα.” 2009. Doctoral Dissertation, University of Patras. Accessed February 24, 2020. http://nemertes.lis.upatras.gr/jspui/handle/10889/3419.

MLA Handbook (7^th Edition):

Μπούμπαλη, Σταυρούλα. “Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα.” 2009. Web. 24 Feb 2020.

Vancouver:

Μπούμπαλη �. Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα. [Internet] [Doctoral dissertation]. University of Patras; 2009. [cited 2020 Feb 24]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3419.

Council of Science Editors:

Μπούμπαλη �. Ο ρόλος της ασβεστιοεξαρτώμενης οδού ενεργοποίησης στην παραγωγή ιντερλευκίνης 10 από Τ λεμφοκύτταρα. [Doctoral Dissertation]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3419

Wright State University

29. Iyer, Ashvin. Developing Proteomic and Cytokine Biomarkers for Vulvodynia.

Degree: MS, Pharmacology and Toxicology, 2015, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1435588817

► Vulvodynia is a chronic, heterogeneous, and multifactorial disease. This condition may affect up to 18 percent of the female population including Caucasians, African Americans, Africans… (more)

▼ Vulvodynia is a chronic, heterogeneous, and multifactorial disease. This condition may affect up to 18 percent of the female population including Caucasians, African Americans, Africans and Hispanics particularly those sexually active at child bearing age. The etiology of this condition is complex and multifactorial and it is frequently accompanied by physical disabilities, psychological distress and sexual dysfunction. Clinically, vulvodynia can be generalized or localized and pain can be provoked or unprovoked. Patients may also describe vulvar paresthesias or dysesthesias that may last hours. The International Society for the Study of Vulvar Disease (ISSVD) recognizes vulvar pain related to a specific disorder (infectious, inflammatory, neoplastic or neurologic) and vulvodynia (pain due to nonspecific etiology). In the case of vulvodynia, even the basic biological processes involved in the condition are unclear and further investigation of these processes is vital for constructing a substantive knowledge base. While recent research activities have created a foundation and enhanced our knowledge base, our understanding of the etiology and pathology of this condition is largely incomplete. Reports suggest a strong link that vulvodynia initiates a strong inflammatory reaction that is mediated by cytokines. In our study, we measured the levels of 27 cytokines that may be involved in this inflammatory response in the vaginal milieu of vulvodynia patients. For our study, we received 15 vaginal swabs from patients diagnosed with vulvodynia and 5 vaginal swabs from healthy patients with no signs and previous history of this disease. Also the application of 2D-Difference in Gel Electrophoresis to study the presence and expression of different proteins in the vaginal milieu of vulvodynia patients has shown promising results. Our results indicate a more than 10-fold increase in IL-1ra levels (p<0.03), a 7-fold increase in IL-12 levels (p<0.04), a 3-fold increase in PDGF-bb (p<0.04) levels, a 9-fold increase in VEGF levels (p<0.02) and a 2-fold decrease in IL-17 levels in vulvodynia patients compared to healthy controls. Our results indicate the presence of a strong inflammatory response involved in vulvodynia. Our proteomic studies indicate alterations in the normal proteomic levels in the vaginal milieu of vulvodynia patients compared to healthy controls. Advisors/Committee Members: Cool, David (Advisor).

Subjects/Keywords: Pharmacology; Vulvodynia, Inflammation, Cytokines, Proteomics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Iyer, A. (2015). Developing Proteomic and Cytokine Biomarkers for Vulvodynia. (Masters Thesis). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1435588817

Chicago Manual of Style (16^th Edition):

Iyer, Ashvin. “Developing Proteomic and Cytokine Biomarkers for Vulvodynia.” 2015. Masters Thesis, Wright State University. Accessed February 24, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1435588817.

MLA Handbook (7^th Edition):

Iyer, Ashvin. “Developing Proteomic and Cytokine Biomarkers for Vulvodynia.” 2015. Web. 24 Feb 2020.

Vancouver:

Iyer A. Developing Proteomic and Cytokine Biomarkers for Vulvodynia. [Internet] [Masters thesis]. Wright State University; 2015. [cited 2020 Feb 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1435588817.

Council of Science Editors:

Iyer A. Developing Proteomic and Cytokine Biomarkers for Vulvodynia. [Masters Thesis]. Wright State University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1435588817

University of Aberdeen

30. Liu, Yu. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.

Degree: School of Medicine and Dentistry., 2008, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer

Subjects/Keywords: Macrophages.; Cytokines.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2008). The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Liu, Yu. “The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.” 2008. Doctoral Dissertation, University of Aberdeen. Accessed February 24, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Liu, Yu. “The role of suppressors of cytokine signalling 1 and 3 in macrophage activation.” 2008. Web. 24 Feb 2020.

Vancouver:

Liu Y. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. [Internet] [Doctoral dissertation]. University of Aberdeen; 2008. [cited 2020 Feb 24]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer.

Council of Science Editors:

Liu Y. The role of suppressors of cytokine signalling 1 and 3 in macrophage activation. [Doctoral Dissertation]. University of Aberdeen; 2008. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24848 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=24848&custom_att_2=simple_viewer

Open Access Theses and Dissertations