subject:(Cysteine protease)

NSYSU

1. Lin, Chia-hung. Ectopic expression of sweet potato cysteine protease SPCP2 promotes earlier flowering and enhances drought stress tolerance.

Degree: Master, Biological Sciences, 2010, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0617110-084745

► Sweet potato SPCP2 is a full-length cDNA isolated from senescent leaves and encodes a putative papain-like cysteine protease. The SPCP2 contained 1101 nucleotides (366 amino… (more)

Subjects/Keywords: cysteine protease; sweet potato

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APA (6^th Edition):

Lin, C. (2010). Ectopic expression of sweet potato cysteine protease SPCP2 promotes earlier flowering and enhances drought stress tolerance. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0617110-084745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Chia-hung. “Ectopic expression of sweet potato cysteine protease SPCP2 promotes earlier flowering and enhances drought stress tolerance.” 2010. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0617110-084745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Chia-hung. “Ectopic expression of sweet potato cysteine protease SPCP2 promotes earlier flowering and enhances drought stress tolerance.” 2010. Web. 23 Jan 2021.

Vancouver:

Lin C. Ectopic expression of sweet potato cysteine protease SPCP2 promotes earlier flowering and enhances drought stress tolerance. [Internet] [Thesis]. NSYSU; 2010. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0617110-084745.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin C. Ectopic expression of sweet potato cysteine protease SPCP2 promotes earlier flowering and enhances drought stress tolerance. [Thesis]. NSYSU; 2010. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0617110-084745

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

2. Schumann, Nicholas. Inhibition of serine and cysteine proteases by peptidomimetic inhibitors.

Degree: 2017, University of Adelaide

URL: http://hdl.handle.net/2440/126464

► Proteases are responsible for the hydrolysis of proteins and peptides and have been implicated in the development of various diseases. Herein describes the design and… (more)

▼ Proteases are responsible for the hydrolysis of proteins and peptides and have been implicated in the development of various diseases. Herein describes the design and synthesis of reversible peptidomimetic inhibitors of serine and cysteine protease for the control of protease activity. Proteases recognise substrates’ secondary peptide structure which conforms to a saw-tooth arrangement of amino acids, known as an extended β-strand geometry. This property has led to the development of potent inhibitors which mimic this conformation. Chapter two discusses defining β-strand geometry in inhibitors, while maintaining key substituents necessary for recognition by protease hosts, by the replacement of N-terminal residues with heterocyclic constraints. The inclusion of a heterocycle both enforces backbone β-strand conformation while increasing inhibitor bioavailability and metabolic stability. A series of peptidomimetic inhibitors containing heterocycles, including pyrrole, furan, thiophene and pyridine were synthesised, and the associated inhibitory activities against a model serine protease, α-Chymotrypsin, determined in proteolytic assays. Of the series of heterocycles, pyrrole was determined to be the optimum heterocycle for inclusion in inhibitors of this class. Extension of this concept of constraint was investigated in the approach towards heterocycle-containing macrocycles constructed by ring-closing metathesis for the inhibition of cysteine Cathepsin proteases, where the introduction of a macrocyclic tether couples with the heterocyclic constraint to define the desired backbone β-strand geometry. Pyrrole-containing macrocycle 2.47 was constructed by ring-closing metathesis, but was found to be unstable to hydrogenation conditions. A similar pyridine-containing macrocycle 2.61 was successfully synthesised and hydrogenated, but was unable to be functionalised with appropriate C-terminal residues due to its poor solubility profile. Chapter three details the design and synthesis of a series of peptidomimetic inhibitors of the serine protease Hip1 which has been implicated with a host innate immune response pathway of Mycobacterium tuberculosis. Described is the synthesis of a library of tripeptides containing an electrophilic boronic ester at the C-terminus for reversible covalent attachment to the Hip1 active site and the associated inhibitory assay data. A lead compound, 3.23, which has exceptional potency of inhibition (low nM activity) was discovered, from which highly potent derivatives featuring a C-terminal aldehyde 3.44, α-keto heterocycle 3.49, and α-keto ester 3.58 were established. Further refinement of these inhibitors presents an opportunity for the development of therapeutics for the treatment of tuberculosis. Advisors/Committee Members: Abell, Andrew (advisor), George, Jonathan (advisor), School of Physical Sciences (school).

Subjects/Keywords: Protease; inhibitors; peptidomimetic; serine; cysteine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schumann, N. (2017). Inhibition of serine and cysteine proteases by peptidomimetic inhibitors. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/126464

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schumann, Nicholas. “Inhibition of serine and cysteine proteases by peptidomimetic inhibitors.” 2017. Thesis, University of Adelaide. Accessed January 23, 2021. http://hdl.handle.net/2440/126464.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schumann, Nicholas. “Inhibition of serine and cysteine proteases by peptidomimetic inhibitors.” 2017. Web. 23 Jan 2021.

Vancouver:

Schumann N. Inhibition of serine and cysteine proteases by peptidomimetic inhibitors. [Internet] [Thesis]. University of Adelaide; 2017. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2440/126464.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schumann N. Inhibition of serine and cysteine proteases by peptidomimetic inhibitors. [Thesis]. University of Adelaide; 2017. Available from: http://hdl.handle.net/2440/126464

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. 麥田, 菜穂. Comparison of the use of tablets containing a cysteine protease actinidin and tongue brushes for reduction of the bacterial load on the tongue : システインプロテアーゼであるアクチニジンを含有したタブレットと舌ブラシを比較したときの舌背上細菌数減少について.

Degree: 博士（歯学）, 2017, Osaka Dental University / 大阪歯科大学

URL: http://id.nii.ac.jp/1392/00000146/

►

Tongue coating as an oral biofilm is responsible for 60% of all halitosis cases. It also in terferes with gustatory perception, and is associated with… (more)

▼

Tongue coating as an oral biofilm is responsible for 60% of all halitosis cases. It also in terferes with gustatory perception, and is associated with periodontal disease and pneu monia. As it is difficult to remove, the development of new cleaning methods is being promoted. We studied tablets containing actinidin, a cysteine protease, which has been reported to effectively remove this coating on the tongue. As tablets placed in the oral cavity are usually abraded by the tongue and palate to promote their dissolution, their ef fect in the elderly is likely to be reduced because of decreased motor function of the tongue. However, there is no literature discussing this effect in the elderly or comparing tablets with tongue brushes. To evaluate the efficacy of tablets containing actinidin on reducing the bacterial load on the tongue, we compared the effect of tablets, tongue brushes, and a combination of both on reduction of the bacterial load on the tongue. Tongue cleaning was performed for elderly individuals requiring assistance or care (the elderly group) and healthy young adults (the young group) using three methods : two tablets containing actinidin (the tablet method), brushing with a tongue brush (the brush ing method), and the combined use of both (the combined method). The bacterial load on the tongue dorsum was measured before treatment, immediately after treatment, and at 1 and 2 hours after treatment. With the brushing method, neither the elderly nor young groups showed significant changes in the bacterial load until 2 hours after treatment. In contrast, a marked decrease in the bacterial load was observed in both groups immedi ately after treatment using the tablets, and immediately after and at 2 hours after treat ment using the combined method. The tablets were particularly effective for the young group, and decreases in the bacterial load were observed immediately and at 1 hour after treatment when they were administered alone or in combination with the tongue brush. Furthermore, in the young group, the bacterial load immediately after and at 1 hour after treatment varied between the tablet and brushing methods. In contrast, in the elderly group, although the bacterial load was significantly reduced with the tablet method, this decrease was not observed when treatment was combined with the tongue brush. In both groups, the decrease in the bacterial load was more marked even at 2 hours after treatment when the combined use was compared with the brushing method alone. These results demonstrated that tablets containing cysteine pro tease are more effective than tongue brushes for reduction of the bacterial load, and that the effect lasts longer when the two methods are combined.

2016年度

Subjects/Keywords: Actinidin｜Cysteine protease｜Tablet｜Bacterial load｜Tongue

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

麥田, �. (2017). Comparison of the use of tablets containing a cysteine protease actinidin and tongue brushes for reduction of the bacterial load on the tongue : システインプロテアーゼであるアクチニジンを含有したタブレットと舌ブラシを比較したときの舌背上細菌数減少について. (Thesis). Osaka Dental University / 大阪歯科大学. Retrieved from http://id.nii.ac.jp/1392/00000146/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

麥田, 菜穂. “Comparison of the use of tablets containing a cysteine protease actinidin and tongue brushes for reduction of the bacterial load on the tongue : システインプロテアーゼであるアクチニジンを含有したタブレットと舌ブラシを比較したときの舌背上細菌数減少について.” 2017. Thesis, Osaka Dental University / 大阪歯科大学. Accessed January 23, 2021. http://id.nii.ac.jp/1392/00000146/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

麥田, 菜穂. “Comparison of the use of tablets containing a cysteine protease actinidin and tongue brushes for reduction of the bacterial load on the tongue : システインプロテアーゼであるアクチニジンを含有したタブレットと舌ブラシを比較したときの舌背上細菌数減少について.” 2017. Web. 23 Jan 2021.

Vancouver:

麥田 �. Comparison of the use of tablets containing a cysteine protease actinidin and tongue brushes for reduction of the bacterial load on the tongue : システインプロテアーゼであるアクチニジンを含有したタブレットと舌ブラシを比較したときの舌背上細菌数減少について. [Internet] [Thesis]. Osaka Dental University / 大阪歯科大学; 2017. [cited 2021 Jan 23]. Available from: http://id.nii.ac.jp/1392/00000146/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

麥田 �. Comparison of the use of tablets containing a cysteine protease actinidin and tongue brushes for reduction of the bacterial load on the tongue : システインプロテアーゼであるアクチニジンを含有したタブレットと舌ブラシを比較したときの舌背上細菌数減少について. [Thesis]. Osaka Dental University / 大阪歯科大学; 2017. Available from: http://id.nii.ac.jp/1392/00000146/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Chicago

4. Siklos, Marton I. Covalent Enzyme Inhibitors in Drug Design.

Degree: 2016, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/20226

► Epoxysuccinates have shown promising activity as irreversible inhibitors of calpain and cathepsin in indications from cancer to Alzheimer’s disease (AD). Their poor pharmacokinetic properties have… (more)

▼ Epoxysuccinates have shown promising activity as irreversible inhibitors of calpain and cathepsin in indications from cancer to Alzheimer’s disease (AD). Their poor pharmacokinetic properties have been addressed by use of simple epoxysuccinate ester prodrugs in preclinical and clinical trials. A prodrug strategy requires the prodrug to be converted efficiently to the drug molecule and for the prodrug itself not to engage off-target biomolecules. We observe that the epoxysuccinate esters are both more reactive and provide an alternative reaction pathway for covalent modification of biomolecules. Reactivity was studied towards GSH, glutathione-S-transferase (GST-P1), and in plasma. The parent epoxysuccinates are stable, whereas ester prodrugs undergo ready base-catalyzed reaction. The enhanced thiophilic reactivity of the neutral epoxide forces a dominant reaction pathway that does not yield the parent drug. This unappreciated reactivity of epoxysuccinyl prodrugs creates challenges in drug design and must be taken into account in interpretation of preclinical and clinical results, since these prodrugs will engage targets other than the cysteine proteases that the parent drug is designed selectively to inhibit. These findings make it obvious that epoxysuccinate esters are unsuitable as clinical candidates due to off-target reactivity. To obtain clinical leads maintaining the desirable pro-cognitive effects of the compounds but lacking the general chemical reactivity, several routes can be taken. Firstly, the electrophilic warhead can be modified in order to solve the problem of high off-target reactivity. A tight-binding reversible inhibitor would be superior in many respects to an irreversible inhibitor. Since a peptidomimetic recognition group is available that confers potent binding to the target enzymes, an alternative electrophile has the potential of solving the problems associated with the epoxysuccinate reactivity. A library of molecular probes was synthesized with the goal of maintaining potent inhibition of target cysteine proteases with attenuated reactivity towards general nucleophiles. An increased membrane permeability compared to the epoxysuccinates through the absence of negative charge was also desired. Several novel electrophilic warheads as well as groups known to react with active cysteines were explored with the intent to identify inhibitors of the target enzymes, calpain 1 and cathepsin B. Advisors/Committee Members: Thatcher, Gregory R. (advisor), Bruzik, Karol S. (committee member), Petukhov, Pavel A. (committee member), Thomas, Douglas D. (committee member), Driver, Tom (committee member).

Subjects/Keywords: cysteine protease inhibitors; calpain; cathepsin B; epoxysuccinate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Siklos, M. I. (2016). Covalent Enzyme Inhibitors in Drug Design. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/20226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Siklos, Marton I. “Covalent Enzyme Inhibitors in Drug Design.” 2016. Thesis, University of Illinois – Chicago. Accessed January 23, 2021. http://hdl.handle.net/10027/20226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Siklos, Marton I. “Covalent Enzyme Inhibitors in Drug Design.” 2016. Web. 23 Jan 2021.

Vancouver:

Siklos MI. Covalent Enzyme Inhibitors in Drug Design. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10027/20226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Siklos MI. Covalent Enzyme Inhibitors in Drug Design. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/20226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Technology, Sydney

5. Dowdell, SN. Modulation of macrophage function and immune response by a helminth-derived cysteine protease.

Degree: 2013, University of Technology, Sydney

URL: http://hdl.handle.net/10453/23473

► Helminth-derived excretory/secretory (ES) products have been demonstrated to mediate the anti-inflammatory/regulatory environment associated with helminth infection (for a review see Allen et al. 2011). The… (more)

▼ Helminth-derived excretory/secretory (ES) products have been demonstrated to mediate the anti-inflammatory/regulatory environment associated with helminth infection (for a review see Allen et al. 2011). The ES products of helminths have been exploited for therapeutic benefit in both murine and human models of autoimmune diseases (Zaccone et al. 2003; Zheng et al. 2008; Motomura et al. 2009; Ruyssers et al. 2009; Johnston et al. 2010; Cancado et al. 2011; Carranza et al. 2012; Kuijk et al. 2012). In our laboratory, the ES products of the liver fluke trematode, Fasciola hepatica, have been shown to prevent autoimmune type 1 diabetes in a murine model (Lund et al. in preparation). Disease prevention was associated with the initiation and perpetuation of anti-inflammatory/regulatory immune responses, including the generation of alternatively activated macrophages, regulatory T cells and regulatory B cell populations (Lund et al. in preparation). Nevertheless, the individual molecular components within the ES responsible for these phenomenon are unknown. Therefore, HPLC fractionation of the ES products of Fasciola hepatica was undertaken. This revealed a number of components with immune-modulatory effects. One of these Fasciola hepatica products is a cysteine protease, cathepsin L1 (FhCL1), and in fact it comprises a large proportion of the total ES products. In mice, FhCL1 suppresses pro-inflammatory immune responses through cleavage of toll-like receptor (TLR)-3, resulting in modulation of cell signalling in peritoneal macrophages (Donnelly et al. 2010). This thesis therefore examines the effect of FhCL1 in human monocyte-derived macrophages. FhCL1 was shown to enhance expression of pro-inflammatory cytokines IL-6 and IL-8 in response to lipopolysaccharide. This was associated with the up-regulation of surface CD14, and the activation of TLR4 cell signalling via both the myeloid differentiation primary response gene 88 (MyD88)-dependent and toll-interleukin-1 receptor-domain-containing adaptor-inducing interferon-β (TRIF)-dependent signalling pathways. Furthermore, expression of IL-10 and co-stimulatory molecule CD86 was down-regulated in FhCL1-treated human monocyte-derived macrophages, and this was attributed to suppression of late endosomal TRIF-dependent signalling, with down-regulation of TRAF3. Although, FhCL1 modulated TLR signalling in human and murine macrophages, and suppressed TRIF-dependent signalling in both human and mouse macrophages, FhCL1 enhanced pro-inflammatory cytokine expression in human monocyte-derived macrophages. Therefore, FhCL1 modulates immune responses in human monocyte-derived macrophages, albeit differently from murine peritoneal macrophages. Furthermore, while FhCL1 degraded TLR3 in murine peritoneal macrophages, FhCL1 had no effect on TLR3 or TLR4 expression or localisation in human monocyte-derived macrophages. However, treatment with FhCL1 was shown to suppress the uptake of lipopolysaccharide (LPS) by human macrophages, which appeared to correlate with altered α-tubulin…

Subjects/Keywords: Helminths.; Macrophages.; Cysteine protease.; Immune response.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dowdell, S. (2013). Modulation of macrophage function and immune response by a helminth-derived cysteine protease. (Thesis). University of Technology, Sydney. Retrieved from http://hdl.handle.net/10453/23473

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dowdell, SN. “Modulation of macrophage function and immune response by a helminth-derived cysteine protease.” 2013. Thesis, University of Technology, Sydney. Accessed January 23, 2021. http://hdl.handle.net/10453/23473.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dowdell, SN. “Modulation of macrophage function and immune response by a helminth-derived cysteine protease.” 2013. Web. 23 Jan 2021.

Vancouver:

Dowdell S. Modulation of macrophage function and immune response by a helminth-derived cysteine protease. [Internet] [Thesis]. University of Technology, Sydney; 2013. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10453/23473.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dowdell S. Modulation of macrophage function and immune response by a helminth-derived cysteine protease. [Thesis]. University of Technology, Sydney; 2013. Available from: http://hdl.handle.net/10453/23473

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Missouri – Columbia

6. Lichtenauer, Anna Marie. The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes.

Degree: 2013, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/38540

► [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The cell permeable cysteine peptidase inhibitor, E-64, has shown utility as a potent and irreversible… (more)

▼ [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The cell permeable cysteine peptidase inhibitor, E-64, has shown utility as a potent and irreversible inhibitor for many cysteine proteases. Consequently, it has been studied in several model systems, including ones involving oocyte and embryo culture. In cattle, administration of E-64 during oocyte IVM or embryo culture resulted in improvements in both oocyte maturation and embryo development. In this study the effects of E-64 were investigated in a porcine IVM/IVF system. Significantly increased rates of oocyte maturation were recorded for COCs treated with E-64. In addition, an increase in cleavage rates was found. However, blastocyst development was not altered. Furthermore, in vitro maturation of porcine COCs using E-64 was performed with COCs segregated into morphologically distinct developmental groups. Those COCs defined as 'good' matured in significantly higher proportions when E-64 was added to the in vitro maturation medium compared to 'good' COCs in the absence of E-64. In addition, 'good' COCs treated with E-64 produced blastocysts with numerically higher total cell counts. In vitro matured COC and oocyte mRNA was extracted and compared to in vivo derived oocyte mRNA. There was a significant difference in cathepsin K mRNA abundance between in vitro matured oocytes and in vivo produced oocytes. However, there was no significant difference in cathepsin B mRNA between the samples, despite that such a difference had been expected based on previous reports in bovine oocytes. In vitro maturation with cathepsin B and cathepsin K specific inhibitors produced oocytes that reached MII at percentages consistent with those of untreated oocytes. Protein expression was used to determine if cathepsin B was being affected at the protein level. Western blots revealed cathepsin B in COCs, but not in denuded oocytes. Additionally, immunocytochemistry verified these results by showing cathepsin B localization in cumulus cells but not in the denuded oocyte. Advisors/Committee Members: Green, Jonathan A., 1969- (advisor).

Subjects/Keywords: oocyte maturation; embryo development; cathepsin; cysteine protease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lichtenauer, A. M. (2013). The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/38540

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lichtenauer, Anna Marie. “The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes.” 2013. Thesis, University of Missouri – Columbia. Accessed January 23, 2021. http://hdl.handle.net/10355/38540.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lichtenauer, Anna Marie. “The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes.” 2013. Web. 23 Jan 2021.

Vancouver:

Lichtenauer AM. The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes. [Internet] [Thesis]. University of Missouri – Columbia; 2013. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10355/38540.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lichtenauer AM. The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes. [Thesis]. University of Missouri – Columbia; 2013. Available from: http://hdl.handle.net/10355/38540

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Faro, Aline Regis. Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa.

Degree: Mestrado, Física Aplicada, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-15122008-150833/ ;

►

A Xylella fastidiosa é uma bactéria gram-negativa que infecta o xilema das plantas causando muitas vezes a maturação precoce e a diminuição dos frutos. Ela… (more)

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A Xylella fastidiosa é uma bactéria gram-negativa que infecta o xilema das plantas causando muitas vezes a maturação precoce e a diminuição dos frutos. Ela é responsável por importantes perdas na economia, no Brasil é a causadora de doenças de Citrus Variegated Chlorosis (CVC) e a da doença de Pierce nos Estados Unidos. As proteases desempenham funções vitais no ciclo de vida de muitos parasitas, muitas estão envolvidas em processos infecciosos, a Xylellaína é uma cisteíno protease que é diferentemente expressa em cepas patogênicas a não patogênica. A compreensão de seu mecanismo catalítico, através do estudo da sua estrutura e função, pode ajudar no planejamento de inibidores seletivos, potenciais agentes contra as doenças fitopatológicas ocasionadas pela X. fastidiosa. Sua estrutura molecular foi elucidada no Laboratório de Cristalografia de Proteínas e Biologia Estrutural do Instituto de Física de São Carlos (USP), estudos estruturais mostraram que a proteína se apresenta na forma de uma pró-proteína, pois está inativa devido a uma pró-região que bloqueia o sítio catalítico. Também foi verificada a presença de um nucleotídeo na estrutura da Xylellaína próximo a pró-região, como hipótese foi considerada a relação entre o nucleotídeo e o mecanismo de ativação da proteína. A influência do nucleotídeo na atividade funcional da enzima foi constatada através da comparação de ensaios enzimáticos entre a enzima nativa e mutantes. As mutações foram planejadas com a intenção de ocasionar a desestabilização do nucleotídeo, por isso foram mutados os resíduos da pró-região que interagem diretamente com o ele. As mutações foram Fenilalanina 45 (F45), Arginina 43 (R43) e F45/R43, todos os resíduos foram mutados para Alaninas (A). Os resultados mostraram que os valores de Km obtidos para a proteína nativa e suas mutantes apresentaram consideráveis alterações quando comparado entre eles, esse efeito não foi percebido para a eficiência catalítica. Conclui-se que as mutações pouco alteraram a capacidade da enzima converter o subsrato em produto, mas houve significantes alterações no reconhecimento do substrato. Esse resultado corrobora com a hipótese de que a existência do nucleotídeo está relacionada com o mecanismo de ativação da proteína.

Xylella fastidiosa is a Gram-negative bacterium which infects the plant xylem system causing in many cases precocious maturation and diminution of fruits. It is responsible for economically important plant diseases, such as the Citrus Variegated Chlorosis (CVC). Proteases might be involved in the infection process by disrupting plant tissue. Xylellain is a cysteine protease which is differently expressed in strain pathogen and non-pathogen of X. fastidiosa. The 3D structure of xylellain was solved by our group and structural studies show that this protein has a proenzyme form and a ribonucleotideo close to the amine terminal region. Our hypothesis is that protein-nucleotide interactions are related to xylellains activation mechanism. To evaluate the influence of the nucleotide in the…

Advisors/Committee Members: Oliva, Glaucius.

Subjects/Keywords: Cisteíno protease; Cysteine protease; Pró-enzima; Proenzyme; Xylella fastidiosa; Xyllela fastidiosa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Faro, A. R. (2008). Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/76/76132/tde-15122008-150833/ ;

Chicago Manual of Style (16^th Edition):

Faro, Aline Regis. “Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa.” 2008. Masters Thesis, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-15122008-150833/ ;.

MLA Handbook (7^th Edition):

Faro, Aline Regis. “Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa.” 2008. Web. 23 Jan 2021.

Vancouver:

Faro AR. Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa. [Internet] [Masters thesis]. University of São Paulo; 2008. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-15122008-150833/ ;.

Council of Science Editors:

Faro AR. Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa. [Masters Thesis]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-15122008-150833/ ;

NSYSU

8. Lai, Jia-fu. A cloned group II phytocystatin SPCPI differentially modulates ethephon and NaCl-induced leaf senescence in sweet potato (Ipomoea batatas (L.) Lam.).

Degree: Master, Biological Sciences, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0725114-135906

► Plant phytocystatins are cysteine protease inhibitors and generally classified into three groups: group I ( ca. 12-16 kDa ), group 2 ( ca. 23 kDa… (more)

▼ Plant phytocystatins are cysteine protease inhibitors and generally classified into three groups: group I ( ca. 12-16 kDa ), group 2 ( ca. 23 kDa ) and group 3 ( ca. 80 kDa ). Phytocystatins play diverse physiological roles and function in cope with plant developmental cues and environmental stress responses. In this research, a full-length cDNA, SPCPI, has been cloned from sweet potato senescent leaves with PCR-based subtractive hybridization and RACE PCR. Sweet potato SPCPI contains 765 nucleotides ( 254 amino acids ) in its open reading frame ( ORF ), and exhibits high amino acid sequence identities ( ca. 57% to 69% ) with different plant phytocystatins, including Amaranthus hypochondriacus, Brassica oleraceae, Solanum lycopersicum, Petunia hybrid, Sesamum indicum, and Solanum tuberosum. Gene and protein structural analysis showed that the SPCPI-encoded protein contains the unique conserved amino acid sequence motif âLARFAVEEHNKâ of phytocystatin, potentially involved in inhibiting active sites of papain-like ( GG, QVVAG, PW ) and legumain-like ( SNSL ) cysteine proteases. Gene expression patterns showed that phytocystatin SPCPI was significantly enhanced in sprouting storage roots, L3 mature and L4 early senescent leaves, and highly inducible by ethephon, an ethylene-releasing compound, and NaCl. Ethephon and NaCl treatment significantly promoted leaf senescence, H2O2 and MDA elevation. Exogenous purified phytocystatin SPCPI fusion protein significantly mitigated ethephon-mediated leaf senescence, however, remarkably accelerated NaCl-induced leaf senescence. The SPCPI fusion protein-mediated effects could be remarkably antagonized by pre-treatment with anti-SPCPI antibody at room temperature for 2 h or by boiling at 95â for 5 min before its application, and reversed them to similar levels of leaf senescence, H2O2 and MDA elevation caused by ethephon or NaCl alone. In addition, exogenous phytocystatin SPCPI fusion protein alone did not significantly alter dark-induced leaf senescence, H2O2 and MDA elevation. These results conclude that sweet potato group II phytocystatin SPCPI is an ethephon and NaCl-inducible gene, and likely participates in the modulation of ethephon and NaCl-mediated leaf senescence, H2O2 and MDA elevation, which require ethephon or NaCl-inducible interacting components for effective interactions. Advisors/Committee Members: Fu, Hong-Yong (chair), Lin Rong-Fang (chair), Huang Guan-Jhong (chair), Chen, Hsien-Jung (committee member).

Subjects/Keywords: Cysteine protease inhibitor; NaCl; Ethephon; Leaf senescence; sweet potato

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lai, J. (2014). A cloned group II phytocystatin SPCPI differentially modulates ethephon and NaCl-induced leaf senescence in sweet potato (Ipomoea batatas (L.) Lam.). (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0725114-135906

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lai, Jia-fu. “A cloned group II phytocystatin SPCPI differentially modulates ethephon and NaCl-induced leaf senescence in sweet potato (Ipomoea batatas (L.) Lam.).” 2014. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0725114-135906.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lai, Jia-fu. “A cloned group II phytocystatin SPCPI differentially modulates ethephon and NaCl-induced leaf senescence in sweet potato (Ipomoea batatas (L.) Lam.).” 2014. Web. 23 Jan 2021.

Vancouver:

Lai J. A cloned group II phytocystatin SPCPI differentially modulates ethephon and NaCl-induced leaf senescence in sweet potato (Ipomoea batatas (L.) Lam.). [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0725114-135906.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lai J. A cloned group II phytocystatin SPCPI differentially modulates ethephon and NaCl-induced leaf senescence in sweet potato (Ipomoea batatas (L.) Lam.). [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0725114-135906

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

9. Liang, Shu-Hao. Cysteine proteases SPAE and SPCP2 are associated with the degradation of trypsin inhibitor in sweet potato storage root.

Degree: Master, Biological Sciences, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0129113-093123

► ããSweet potato cysteine proteases SPAE and SPCP2 exhibited high amino acid sequence identities with seed vacuolar processing enzyme (VPE) and papain-like cysteine protease, respectively, which… (more)

Subjects/Keywords: Cysteine protease; Globulin; Sprouting; Sweet potato; Storage root; Trypsin inhibitor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liang, S. (2013). Cysteine proteases SPAE and SPCP2 are associated with the degradation of trypsin inhibitor in sweet potato storage root. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0129113-093123

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liang, Shu-Hao. “Cysteine proteases SPAE and SPCP2 are associated with the degradation of trypsin inhibitor in sweet potato storage root.” 2013. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0129113-093123.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liang, Shu-Hao. “Cysteine proteases SPAE and SPCP2 are associated with the degradation of trypsin inhibitor in sweet potato storage root.” 2013. Web. 23 Jan 2021.

Vancouver:

Liang S. Cysteine proteases SPAE and SPCP2 are associated with the degradation of trypsin inhibitor in sweet potato storage root. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0129113-093123.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liang S. Cysteine proteases SPAE and SPCP2 are associated with the degradation of trypsin inhibitor in sweet potato storage root. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0129113-093123

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

10. Krauser, Joel Anderson. Design, synthesis and evaluation of novel inhibitors and fluorogenic substrates for cysteine proteases and metallo proteases.

Degree: PhD, Chemistry, 2001, Georgia Tech

URL: http://hdl.handle.net/1853/30003

Subjects/Keywords: Protease inhibitors; Cysteine proteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krauser, J. A. (2001). Design, synthesis and evaluation of novel inhibitors and fluorogenic substrates for cysteine proteases and metallo proteases. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30003

Chicago Manual of Style (16^th Edition):

Krauser, Joel Anderson. “Design, synthesis and evaluation of novel inhibitors and fluorogenic substrates for cysteine proteases and metallo proteases.” 2001. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/30003.

MLA Handbook (7^th Edition):

Krauser, Joel Anderson. “Design, synthesis and evaluation of novel inhibitors and fluorogenic substrates for cysteine proteases and metallo proteases.” 2001. Web. 23 Jan 2021.

Vancouver:

Krauser JA. Design, synthesis and evaluation of novel inhibitors and fluorogenic substrates for cysteine proteases and metallo proteases. [Internet] [Doctoral dissertation]. Georgia Tech; 2001. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/30003.

Council of Science Editors:

Krauser JA. Design, synthesis and evaluation of novel inhibitors and fluorogenic substrates for cysteine proteases and metallo proteases. [Doctoral Dissertation]. Georgia Tech; 2001. Available from: http://hdl.handle.net/1853/30003

Queensland University of Technology

11. Ranjit, Najju. Characterisation of proteases involved in proteolytic degradation of haemoglobin in the human hookworm Necator americanus.

Degree: 2008, Queensland University of Technology

URL: https://eprints.qut.edu.au/20651/

► With over a billion people infected world wide, hookworms are considered as important human pathogens, particularly in developing countries which have the highest rates of… (more)

▼ With over a billion people infected world wide, hookworms are considered as important human pathogens, particularly in developing countries which have the highest rates of infections. Hookworms reside in the gastrointestinal tract of the host where they continuously feed on blood, leading to conditions such as chronic irondeficiency anaemia. The majority of blood-feeding parasites rely on proteins found in blood to provide many of their nutritional requirements for growth, reproduction and survival. Of the numerous proteins found in blood, haemoglobin (Hb) is one of the most abundant. In order to acquire amino acids for protein synthesis, it is thought that haematophagous parasites degrade Hb using various classes of endo- and exoproteases, in a manner similar to that which occurs in catabolism of proteins in mammalian cellular lysosomes. This study identified and characterised proteases involved in the Hb degradation process in the human hookworm, Necator americanus, in order to identify potential candidate antigens for a vaccine that interrupts blood-feeding. Red blood cells ingested by hookworms are lysed to release Hb, which is cleaved by various proteases into dipeptides or free amino acids and these are taken up through the gut membrane by amino acid transporters. Proteases expressed in the intestinal tract of hookworms are thought to play a major role in this process and would therefore make good targets for vaccine candidates aimed at interrupting blood-feeding. To identify these proteases, adult hookworms (both N. americanus and Ancylostoma caninum) were sectioned and intestinal tissue was dissected via laser microdissection microscopy. RNA extracted from the dissected tissue was used to generate gut-specific cDNA, which then was used to create plasmid libraries. Each library was subjected to shotgun sequencing, and of the 480 expressed sequence tags (ESTs) sequenced from each species, 268 and 276 contigs were assembled from the N. americanus and A. caninum libraries, respectively. Nine percent of N. americanus and 6.5% of A. caninum contigs were considered novel as no homologues were identified in any published/accessible database. The gene ontology (GO) classification system was used to categorise the contigs to predicted biological functions. Only 17% and 38% of N. americanus and A. caninum contigs, respectively, were assigned GO categories, while the rest were classified as being of unknown function. The most highly represented GO categories were molecular functions such as protein binding and catalytic activity. The most abundant transcripts encoded fatty acid binding proteins, C-type lectins and activation associated secreted proteins, indicative of the diversity of functions that occur in this complex organ. Of particular interest to this study were the contigs that encoded for cysteine and metalloproteases, expanding the list of potential N. americanus haemoglobinases. In the N. americanus cDNA library, four contigs encoding for cathepsin B cysteine proteases were identified. Three contigs from the…

Subjects/Keywords: Necator americanus; hookworm; laser microscopy microdissection; haemoglobin degradation; haemoglobinases; cysteine protease; aspartic protease; metalloprotease; intestinal proteases; vaccine candidates

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ranjit, N. (2008). Characterisation of proteases involved in proteolytic degradation of haemoglobin in the human hookworm Necator americanus. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/20651/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ranjit, Najju. “Characterisation of proteases involved in proteolytic degradation of haemoglobin in the human hookworm Necator americanus.” 2008. Thesis, Queensland University of Technology. Accessed January 23, 2021. https://eprints.qut.edu.au/20651/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ranjit, Najju. “Characterisation of proteases involved in proteolytic degradation of haemoglobin in the human hookworm Necator americanus.” 2008. Web. 23 Jan 2021.

Vancouver:

Ranjit N. Characterisation of proteases involved in proteolytic degradation of haemoglobin in the human hookworm Necator americanus. [Internet] [Thesis]. Queensland University of Technology; 2008. [cited 2021 Jan 23]. Available from: https://eprints.qut.edu.au/20651/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ranjit N. Characterisation of proteases involved in proteolytic degradation of haemoglobin in the human hookworm Necator americanus. [Thesis]. Queensland University of Technology; 2008. Available from: https://eprints.qut.edu.au/20651/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

12. Rukamp, Brian John. Design, synthesis, and evaluation of novel thiobenzyl ester substrates and aza-peptide inhibitors for serine and cysteine proteases.

Degree: PhD, Chemistry and Biochemistry, 2003, Georgia Tech

URL: http://hdl.handle.net/1853/5371

Subjects/Keywords: Protease inhibitors; Serine proteinases; Cysteine proteinases; Serine proteinases; Protease inhibitors; Cysteine proteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rukamp, B. J. (2003). Design, synthesis, and evaluation of novel thiobenzyl ester substrates and aza-peptide inhibitors for serine and cysteine proteases. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/5371

Chicago Manual of Style (16^th Edition):

Rukamp, Brian John. “Design, synthesis, and evaluation of novel thiobenzyl ester substrates and aza-peptide inhibitors for serine and cysteine proteases.” 2003. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/5371.

MLA Handbook (7^th Edition):

Rukamp, Brian John. “Design, synthesis, and evaluation of novel thiobenzyl ester substrates and aza-peptide inhibitors for serine and cysteine proteases.” 2003. Web. 23 Jan 2021.

Vancouver:

Rukamp BJ. Design, synthesis, and evaluation of novel thiobenzyl ester substrates and aza-peptide inhibitors for serine and cysteine proteases. [Internet] [Doctoral dissertation]. Georgia Tech; 2003. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/5371.

Council of Science Editors:

Rukamp BJ. Design, synthesis, and evaluation of novel thiobenzyl ester substrates and aza-peptide inhibitors for serine and cysteine proteases. [Doctoral Dissertation]. Georgia Tech; 2003. Available from: http://hdl.handle.net/1853/5371

Georgia Tech

13. Rukamp, Karrie Eileen Adlington. Design and synthesis of inhibitors for serine and cysteine proteases.

Degree: PhD, Chemistry and Biochemistry, 2003, Georgia Tech

URL: http://hdl.handle.net/1853/5426

Subjects/Keywords: Protease inhibitors; Serine proteinases; Cysteine proteinases; Serine proteinases; Protease inhibitors; Cysteine proteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rukamp, K. E. A. (2003). Design and synthesis of inhibitors for serine and cysteine proteases. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/5426

Chicago Manual of Style (16^th Edition):

Rukamp, Karrie Eileen Adlington. “Design and synthesis of inhibitors for serine and cysteine proteases.” 2003. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/5426.

MLA Handbook (7^th Edition):

Rukamp, Karrie Eileen Adlington. “Design and synthesis of inhibitors for serine and cysteine proteases.” 2003. Web. 23 Jan 2021.

Vancouver:

Rukamp KEA. Design and synthesis of inhibitors for serine and cysteine proteases. [Internet] [Doctoral dissertation]. Georgia Tech; 2003. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/5426.

Council of Science Editors:

Rukamp KEA. Design and synthesis of inhibitors for serine and cysteine proteases. [Doctoral Dissertation]. Georgia Tech; 2003. Available from: http://hdl.handle.net/1853/5426

University of Pretoria

14. Van Wyk, Stefan George. Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis .

Degree: 2011, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-09192011-174830/

► Novel conserved amino acid variations of papaya cystatin (PC) were investigated by amino acid substitutions using oryzacystatin-I (OCI) as a model plant cystatin for comparison.… (more)

▼ Novel conserved amino acid variations of papaya cystatin (PC) were investigated by amino acid substitutions using oryzacystatin-I (OCI) as a model plant cystatin for comparison. These amino acid residues in the conserved motifs are involved in binding with cysteine proteases, these include the GG (Gly-Gly) in the N-terminal region for both OCI and PC, the (Q)QVVAG (Gln-Val-Val-Ala-Gly) motif for OCI and (Q)AVVEG (Ala-Val-Val-Glu-Gly) motif for PC in the first inhibitory loop, and the PW (Pro-Trp) motif for OCI and LW (Leu-Trp) motif for PC in the second inhibitory loop. Recombinant OCI and PC mutant proteins were expressed in Escherichia coli and were tested for altered inhibitory activity against commercial cysteine proteases (papain and cathepsin L) and extracts from Colorado potato beetle (Leptinotarsa decemlineata) larvae, from banana weevil larvae (Cosmopolites sordidus) and tobacco leaf extracts (Nicotiana benthamiana). In all tests higher amounts of PC had to be used to obtain similar inhibition levels as OCI. Changing the amino acid Q at position 52 to E in OCI in the first inhibitory loop, had lowered the Ki value of the mutant against the commercial proteases. Concurrently the same amino acid string (EQ) in PC had resulted in a significantly decreased Ki value compared to PC wild-type and other mutants. All other OCI mutants were less efficient than the wild-type OCI, whereas all PC first inhibitory loop mutants had improved inhibitory activity against protease activity with the highest improvement against the protease extracts was found for the substitution of E with A at position 55. This study has shown the importance of the three conserved motifs and that it is possible to improve the binding capacity of a plant cystatins to cysteine protease activity by amino acid substitution using site-directed mutagenesis. By mutating individual amino acid residues in the first binding loop of the relatively “weak” papaya cystatin to amino acid residues found in OCI caused a significant improvement in inhibitory potency of PC. Copyright Advisors/Committee Members: Kunert, Karl J (advisor), Vorster, Barend Juan (advisor).

Subjects/Keywords: Oryzacystatin; Papaya cystatin; Site-directed mutagenesis; Cysteine protease inhibitor; Inhibitor engineering; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Wyk, S. G. (2011). Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-09192011-174830/

Chicago Manual of Style (16^th Edition):

Van Wyk, Stefan George. “Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis .” 2011. Masters Thesis, University of Pretoria. Accessed January 23, 2021. http://upetd.up.ac.za/thesis/available/etd-09192011-174830/.

MLA Handbook (7^th Edition):

Van Wyk, Stefan George. “Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis .” 2011. Web. 23 Jan 2021.

Vancouver:

Van Wyk SG. Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis . [Internet] [Masters thesis]. University of Pretoria; 2011. [cited 2021 Jan 23]. Available from: http://upetd.up.ac.za/thesis/available/etd-09192011-174830/.

Council of Science Editors:

Van Wyk SG. Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis . [Masters Thesis]. University of Pretoria; 2011. Available from: http://upetd.up.ac.za/thesis/available/etd-09192011-174830/

Universidade Federal de Viçosa

15. Eduardo Gomes de Mendonça. Influência de inibidores proteicos e não-proteicos de proteases digestivas e caracterização bioquímico-cinética de cisteíno-proteases parcialmente purificadas de Anticarsia gemmatalis.

Degree: 2012, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4607

► Os inibidores de proteases têm efeito protetor de plantas contra danos causados por vários tipos de pragas agrícolas, inibindo, significativamente, a atividade proteolítica de enzimas… (more)

▼ Os inibidores de proteases têm efeito protetor de plantas contra danos causados por vários tipos de pragas agrícolas, inibindo, significativamente, a atividade proteolítica de enzimas presentes no intestino dos insetos. A ingestão de inibidores de proteases leva os insetos a utilizarem aminoácidos livres na síntese de mais proteases digestivas, com a finalidade de compensar as formas dessas enzimas indisponíveis à catálise, justamente por estas estarem na forma do complexo enzima-inibidor (EI). Consequentemente, ao invés de utilizarem os aminoácidos biodisponíveis para a síntese de proteínas necessárias ao seu crescimento, desenvolvimento, manutenção e reprodução os insetos usam sua reserva aminoacídica para a biossíntese de novas proteases para realizar a hidrólise de sua dieta proteica. A caracterização bioquímica e cinética de enzimas hidrolíticas presentes no intestino de insetos tem sido realizada para se entender o complexo sistema de proteases dos insetos sob o ponto de vista da relação estrutura/função. Dessa forma, os centros ativos de tais enzimas poderão ser mapeados através de cinética enzimática e química de proteínas, com o objetivo de produzir inibidores específicos para cada protease. Neste trabalho foram selecionados os inibidores de proteases SBBI, SKTI e E-64, a fim de avaliar seus efeitos sobre o desenvolvimento larval de Anticarsia gemmatalis e sobre a atividade de algumas enzimas digestivas, tais como proteases totais, serino-proteases tripsinas-like e cisteíno-proteases. Esses inibidores foram adicionados individualmente na dieta artificial na concentração de 0,1% (p/v) e lagartas de 1 ínstar foram alimentadas até completarem seu ciclo larval. Os três inibidores utilizados causaram prejuízo no desenvolvimento de larvas de A. gemmatalis, aumentando o ciclo larval e afetando o ganho de peso dessas larvas até a fase pupal. A concentração de IPs utilizada foi insuficiente para causar efeito inibitório das proteases presentes no intestino de A. gemmatalis. Porém o perfil de proteases coincidiu com o perfil das proteases específicas tripsina-like e cisteíno-proteases, sendo que as serino-proteases apresentaram maior atividade do que as cisteíno-proteases. Foi realizada também a purificação parcial de cisteíno-proteases do intestino médio de Anticarsia gemmatalis utilizando-se precipitação diferencial com 30 60% de (NH4)2SO4 e cromatografia de afinidade com coluna de aprotinina-agarose. As proteínas não ligadas à coluna, ou seja, todas as proteínas presentes no extrato bruto com exceção das serinoproteases ligadas à aprotinina-agarose, que apresentaram atividade proteolítica, foram reunidas num pool enzimático e caracterizadas utilizando o substrato sintético L-BApNA na presença do inibidor de serino-proteases benzamidina. Observaram-se picos de atividade nos valores de pH 4,0, 5,5 e 8,0, sendo o pH 8,0 o de melhor atividade. Com relação à influência da temperatura, observaram-se dois picos mais discretos de atividade a 20C e entre 35 45C, e um pico de atividade pronunciada na faixa entre 55 60C. O KM… Advisors/Committee Members: Camila Rocha da Silva, Raul Narciso Carvalho Guedes, Maria Goreti de Almeida Oliveira, Marcelo Matos Santoro, Thiago Rennó dos Mares Guia, Joel Antônio de Oliveira, Wellington Garcia Campos, Liliane Evangelista Visôtto.

Subjects/Keywords: ENZIMOLOGIA; Inibidores de proteases; Cisteíno-proteases; Anticarsia gemmatalis; Protease inhibitors; Cysteine-proteases; Anticarsia gemmatalis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendonça, E. G. d. (2012). Influência de inibidores proteicos e não-proteicos de proteases digestivas e caracterização bioquímico-cinética de cisteíno-proteases parcialmente purificadas de Anticarsia gemmatalis. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4607

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mendonça, Eduardo Gomes de. “Influência de inibidores proteicos e não-proteicos de proteases digestivas e caracterização bioquímico-cinética de cisteíno-proteases parcialmente purificadas de Anticarsia gemmatalis.” 2012. Thesis, Universidade Federal de Viçosa. Accessed January 23, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4607.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mendonça, Eduardo Gomes de. “Influência de inibidores proteicos e não-proteicos de proteases digestivas e caracterização bioquímico-cinética de cisteíno-proteases parcialmente purificadas de Anticarsia gemmatalis.” 2012. Web. 23 Jan 2021.

Vancouver:

Mendonça EGd. Influência de inibidores proteicos e não-proteicos de proteases digestivas e caracterização bioquímico-cinética de cisteíno-proteases parcialmente purificadas de Anticarsia gemmatalis. [Internet] [Thesis]. Universidade Federal de Viçosa; 2012. [cited 2021 Jan 23]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4607.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mendonça EGd. Influência de inibidores proteicos e não-proteicos de proteases digestivas e caracterização bioquímico-cinética de cisteíno-proteases parcialmente purificadas de Anticarsia gemmatalis. [Thesis]. Universidade Federal de Viçosa; 2012. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4607

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

16. Bester, Christell. Detection of a papaya cysteine proteinase inhibitor under different environmental conditions.

Degree: 2012, University of Johannesburg

URL: http://hdl.handle.net/10210/6145

►

M.Sc.

Proteinases are involved in many cellular reactions involving protein degradation, such as degradation of storage proteins and protein degradation during senescence processes. Their action… (more)

▼

M.Sc.

Proteinases are involved in many cellular reactions involving protein degradation, such as degradation of storage proteins and protein degradation during senescence processes. Their action can be inhibited by proteinase inhibitors. Information is still limited about the regulation of these inhibitors in plants and their possible interaction with proteinases under stress conditions. To obtain a better understanding of the physiological role of a proteinase inhibitor in plants under stress, the expression of a papaya cysteine proteinase inhibitor (cystatin) and its relation to proteinase expression was investigated in more detail. For this purpose, expression of the inhibitor was studied in papaya plants exposed to different physiological stress conditions, such as high/low temperature, and treatment with selected chemicals, such as glutathione, OTC (L-2- Oxothiazolidine-4-carboxylate), bestatin ([(2S, 3R)-3-amino-2-hydroxy-4-phenyl butanoylj-L-leu) and 2.4-D (2,4-dichiorophenoxyacetic acid). Using detection tools like activity gel electrophoresis, immunoblotting and enzymatic assays, the production of the cystatin under stress was monitored in different papaya explants, such as roots, leaves and embryos. Inhibitor production increased under different stress conditions when compared to untreated controls. However, this increase was not dramatic in any of the stresses applied. Exact quantification of the increase by using immunoblotting as the only specific tool to determine cystatin expression, was difficult. Neither activity gel electrophoresis nor enzymatic assays were successful to further quantify the exact cystatin levels. Higher cystatin expression was accompanied with a decrease in proteinase activity. Transgenic tobacco plants carrying the gene for a rice cystatin had a significantly lower cysteine proteinase activity when compared to non-transgenic tobacco plants after prolonged cold stress. Furthermore, protein degradation and leaf yellowing as a consequence of cold treatment were prevented in transgenic plants. An attempt to obtain a transformed papaya plant to study silencing of cystatin expression under stress was unsuccessful. In this study, the protective role of a cystatin in cold stress was described for the first time.

Subjects/Keywords: Cysteine proteinases – Research; Protease inhibitors – Research; Proteinase – Inhibitors – Research; Proteolytic enzymes – Inhibitors – Research.; Papaya – Research

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bester, C. (2012). Detection of a papaya cysteine proteinase inhibitor under different environmental conditions. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/6145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bester, Christell. “Detection of a papaya cysteine proteinase inhibitor under different environmental conditions.” 2012. Thesis, University of Johannesburg. Accessed January 23, 2021. http://hdl.handle.net/10210/6145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bester, Christell. “Detection of a papaya cysteine proteinase inhibitor under different environmental conditions.” 2012. Web. 23 Jan 2021.

Vancouver:

Bester C. Detection of a papaya cysteine proteinase inhibitor under different environmental conditions. [Internet] [Thesis]. University of Johannesburg; 2012. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10210/6145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bester C. Detection of a papaya cysteine proteinase inhibitor under different environmental conditions. [Thesis]. University of Johannesburg; 2012. Available from: http://hdl.handle.net/10210/6145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

17. Van Wyk, Stefan George. Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis.

Degree: Plant Science, 2011, University of Pretoria

URL: http://hdl.handle.net/2263/28047

► Novel conserved amino acid variations of papaya cystatin (PC) were investigated by amino acid substitutions using oryzacystatin-I (OCI) as a model plant cystatin for comparison.… (more)

▼ Novel conserved amino acid variations of papaya cystatin (PC) were investigated by amino acid substitutions using oryzacystatin-I (OCI) as a model plant cystatin for comparison. These amino acid residues in the conserved motifs are involved in binding with cysteine proteases, these include the GG (Gly-Gly) in the N-terminal region for both OCI and PC, the (Q)QVVAG (Gln-Val-Val-Ala-Gly) motif for OCI and (Q)AVVEG (Ala-Val-Val-Glu-Gly) motif for PC in the first inhibitory loop, and the PW (Pro-Trp) motif for OCI and LW (Leu-Trp) motif for PC in the second inhibitory loop. Recombinant OCI and PC mutant proteins were expressed in Escherichia coli and were tested for altered inhibitory activity against commercial cysteine proteases (papain and cathepsin L) and extracts from Colorado potato beetle (Leptinotarsa decemlineata) larvae, from banana weevil larvae (Cosmopolites sordidus) and tobacco leaf extracts (Nicotiana benthamiana). In all tests higher amounts of PC had to be used to obtain similar inhibition levels as OCI. Changing the amino acid Q at position 52 to E in OCI in the first inhibitory loop, had lowered the Ki value of the mutant against the commercial proteases. Concurrently the same amino acid string (EQ) in PC had resulted in a significantly decreased Ki value compared to PC wild-type and other mutants. All other OCI mutants were less efficient than the wild-type OCI, whereas all PC first inhibitory loop mutants had improved inhibitory activity against protease activity with the highest improvement against the protease extracts was found for the substitution of E with A at position 55. This study has shown the importance of the three conserved motifs and that it is possible to improve the binding capacity of a plant cystatins to cysteine protease activity by amino acid substitution using site-directed mutagenesis. By mutating individual amino acid residues in the first binding loop of the relatively “weak” papaya cystatin to amino acid residues found in OCI caused a significant improvement in inhibitory potency of PC. Copyright Advisors/Committee Members: Kunert, Karl J. (advisor), Vorster, Barend Juan (coadvisor).

Subjects/Keywords: Oryzacystatin; Papaya cystatin; Site-directed mutagenesis; Cysteine protease inhibitor; Inhibitor engineering; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Wyk, S. G. (2011). Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/28047

Chicago Manual of Style (16^th Edition):

Van Wyk, Stefan George. “Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis.” 2011. Masters Thesis, University of Pretoria. Accessed January 23, 2021. http://hdl.handle.net/2263/28047.

MLA Handbook (7^th Edition):

Van Wyk, Stefan George. “Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis.” 2011. Web. 23 Jan 2021.

Vancouver:

Van Wyk SG. Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis. [Internet] [Masters thesis]. University of Pretoria; 2011. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2263/28047.

Council of Science Editors:

Van Wyk SG. Improving the inhibitory potency of papaya cystatin, using site-directed mutagenesis. [Masters Thesis]. University of Pretoria; 2011. Available from: http://hdl.handle.net/2263/28047

18. ADLER, NIKOLETTA. Development of Novel Anti-Trypanosomal Compounds via Computational, In Vitro and In Vivo Methods.

Degree: School of Biochemistry & Immunology. Discipline of Biochemistry, 2018, Trinity College Dublin

URL: http://hdl.handle.net/2262/82830

► Human African Trypanosomiasis is a neglected parasitic disease caused by Trypanosoma brucei, which if left untreated eventually leads to coma and death. With no successful… (more)

▼ Human African Trypanosomiasis is a neglected parasitic disease caused by Trypanosoma brucei, which if left untreated eventually leads to coma and death. With no successful vaccine to date, treatment relies on chemotherapy. The currently used 5 parenteral drugs (melarsoprol, pentamidine, suramin, eflornithine, nifurtimox) all have non-negligible toxicity and are complex to use, requiring close monitoring. Additionally, there is emergence of parasite resistance to these drugs, in particular to melarsoprol, the only effective drug for the late, CNS invasive stage of both human infective T. brucei subspecies. Thus, there is a need to develop more tolerable and efficacious treatments. This study investigated the efficacy of various new therapeutic leads as anti-trypanosomal agents, including some repurposed and already FDA-approved. First, a series of vinyl sulfones (40 compounds) from traditional drug discovery pipelines were evaluated for their ability to disrupt the life cycle of trypanosomes in a whole-cell screen of T. b. brucei via a standard Alamar Blue assay. Out of the series tested, 13 compounds were identified with nanomolar activity, making them more potent than the original K777 vinyl sulfone, a promising therapeutic lead against the similar protozoan disease American Trypanosomiais. Additionally, through fluorescence imaging, this study showed that the cysteine protease inhibitor subcellular localisation was in the lysozyme as expected, but surprisingly it was present in nucleus and kinetoplast as well. Further new leads were identified through drug repurposing methods including: a) similarity searches between databases of anti-trypanocidal compounds vs drugs with blood-brain barrier passing capabilities, and b) identifying compounds that modulate the activity of a conserved parasite target, in this case ergosterol, between parasites of similar biology and genomic sequences (T. brucei, T. cruzi, Leishmania). For the first time, the study demonstrated that the antifungals amphotericin B (AmpB) and SPK-843 as well as, fingolimod (FTY720), the first FDA-approved oral agent for the management of relapsing forms of multiple sclerosis, its phosphorylated derivative FTY720P, and endogenous structural mimics sphingosine and sphingosine-1-phosphateare (S1P) were able to kill T. b. brucei in vitro at low and submicromolar concentrations. Specifically, SPK-843, which was tested because of its structural similarity to AmpB, induced parasite death more rapidly and with a greater efficacy than AmpB. The mode of action following AmpB treatment involved membrane permeabilisation as demonstrated by the entry of fluorescent propidium iodide into the cells, possibly via formation of pore-inducing AmpB-ergosterol complexes. Channel formation upon AmpB binding ergosterol was expected as it has been shown in Leishmania and fungal studies. In contrast, there was no membrane disruption detected following SPK-843 treatment. It is proposed here that SPK-843 was able to induce trypansome cell death by simply binding ergosterol, a… Advisors/Committee Members: Knox, Andrew, Nolan, Derek.

Subjects/Keywords: anti-trypnosomal; cysteine protease inhibitor; drug repurposing; Fingolimod; S1P; SPK-843; Amphotericin B

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ADLER, N. (2018). Development of Novel Anti-Trypanosomal Compounds via Computational, In Vitro and In Vivo Methods. (Thesis). Trinity College Dublin. Retrieved from http://hdl.handle.net/2262/82830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ADLER, NIKOLETTA. “Development of Novel Anti-Trypanosomal Compounds via Computational, In Vitro and In Vivo Methods.” 2018. Thesis, Trinity College Dublin. Accessed January 23, 2021. http://hdl.handle.net/2262/82830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ADLER, NIKOLETTA. “Development of Novel Anti-Trypanosomal Compounds via Computational, In Vitro and In Vivo Methods.” 2018. Web. 23 Jan 2021.

Vancouver:

ADLER N. Development of Novel Anti-Trypanosomal Compounds via Computational, In Vitro and In Vivo Methods. [Internet] [Thesis]. Trinity College Dublin; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2262/82830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ADLER N. Development of Novel Anti-Trypanosomal Compounds via Computational, In Vitro and In Vivo Methods. [Thesis]. Trinity College Dublin; 2018. Available from: http://hdl.handle.net/2262/82830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

19. Gheura, Iuliana L. Design, synthesis and evaluation of AZA-peptide epoxides as inhibitors of cysteine proteases.

Degree: PhD, Chemistry, 2002, Georgia Tech

URL: http://hdl.handle.net/1853/30571

Subjects/Keywords: Protease inhibitors; Epoxy compounds; Cysteine proteinases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gheura, I. L. (2002). Design, synthesis and evaluation of AZA-peptide epoxides as inhibitors of cysteine proteases. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30571

Chicago Manual of Style (16^th Edition):

Gheura, Iuliana L. “Design, synthesis and evaluation of AZA-peptide epoxides as inhibitors of cysteine proteases.” 2002. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/30571.

MLA Handbook (7^th Edition):

Gheura, Iuliana L. “Design, synthesis and evaluation of AZA-peptide epoxides as inhibitors of cysteine proteases.” 2002. Web. 23 Jan 2021.

Vancouver:

Gheura IL. Design, synthesis and evaluation of AZA-peptide epoxides as inhibitors of cysteine proteases. [Internet] [Doctoral dissertation]. Georgia Tech; 2002. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/30571.

Council of Science Editors:

Gheura IL. Design, synthesis and evaluation of AZA-peptide epoxides as inhibitors of cysteine proteases. [Doctoral Dissertation]. Georgia Tech; 2002. Available from: http://hdl.handle.net/1853/30571

RMIT University

20. Alsharif, A. Molecular and immunological characterisation of proteins from Anisakis pegreffii and their immune stimulatory effect on the human health system.

Degree: 2015, RMIT University

URL: http://researchbank.rmit.edu.au/view/rmit:161426

► With the fast growth of seafood trading worldwide, the potential health risks of eating contaminated seafood have greatly increased. Biological effects of contaminated seafood are… (more)

▼ With the fast growth of seafood trading worldwide, the potential health risks of eating contaminated seafood have greatly increased. Biological effects of contaminated seafood are associated and caused by a variety of bacteria, viruses, and parasites; this various groups of pathogens results in an extensive diversity of clinical syndromes, each with its own epidemiological characteristics. According to a World Health Organization investigation, more than 1 billion people worldwide are infected with different species of the Ascaris lumbricoides worm parasite (282), which causes serious conditions ranging from mild to lethal. Anisakis parasites in marine fish have imposed a signiﬁcant economic burden, reducing productivity and requiring elaborate and expensive control methods. Nematodes of the family Anisakidae is a major group parasitise fish, mammals, birds and reptiles, with the larval stages of some species. Several species of Anisakis have been found to be parasitic in marine mammals such as whales and dolphins in their adult stages, and their larvae are found in a variety of fish species. Anisakid nematodes have complex life cycles that include invertebrate and vertebrate hosts at various developmental stages during their life. Most reports of anisakiasis infections are associated with eating raw or undercooked ﬁsh that contain larval Anisakis. Humans can also be accidental hosts for larval Anisakis, however they cannot progress their life cycles, but they can frequently cause hypersensitivity IgE-mediated reactions with or without several gastrointestinal manifestations ranging from urticaria to angioedema, called anisakiasis diseases. The development of an allergic reaction mediated by IgE may be against ES and somatic allergens of larval Anisakis. Nuclear ribosomal DNA (rDNA) provides suitable genetic markers for the identification of larval Anisakis. Therefore, the sequences of internal transcribed spacers (ITS-1 and ITS-2) are a powerful approach to identify and distinguish anisakid nematodes (at any developmental stage) for diagnostic or taxonomic purposes, for exploring the genetic composition of larval anisakid populations and for investigating their ecology. Although several allergens have been identified in Anisakis simplex, little information is available for other parasite species infecting fish. Therefore, this project aims to investigate the allergenicity of tropomyosin (Ani p 3) and a cysteine protease inhibitor (Ani p 4) from the sibling species, Anisakis pegreffii, using biochemical, genetic and immunological approaches. Larvae (L3) were harvested manually from tiger flathead fish and identified morphologically using a light microscope. For molecular characterisation and genetic diversity, total genomic DNA was extracted from individual samples and verified by species-specific PCR amplification using the sequences of the first and/or second internal transcribed spacers (ITS-1 and/or ITS-2) of ribosomal DNA (rDNA) as the species-specific genetic markers. For molecular characterisation of…

Subjects/Keywords: Fields of Research; allergenicity of the nematode tropomyosin and cysteine protease inhibitor was evaluated; identification of Tropomyosin in Anisakis pegreffii; Identification of cysteine protease inhibitor in Anisakis pegreffii; Coning of Tropomyosin in Anisakis pegreffii; Cloning of cysteine protease inhibitor in Anisakis pegreffii

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alsharif, A. (2015). Molecular and immunological characterisation of proteins from Anisakis pegreffii and their immune stimulatory effect on the human health system. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:161426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alsharif, A. “Molecular and immunological characterisation of proteins from Anisakis pegreffii and their immune stimulatory effect on the human health system.” 2015. Thesis, RMIT University. Accessed January 23, 2021. http://researchbank.rmit.edu.au/view/rmit:161426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alsharif, A. “Molecular and immunological characterisation of proteins from Anisakis pegreffii and their immune stimulatory effect on the human health system.” 2015. Web. 23 Jan 2021.

Vancouver:

Alsharif A. Molecular and immunological characterisation of proteins from Anisakis pegreffii and their immune stimulatory effect on the human health system. [Internet] [Thesis]. RMIT University; 2015. [cited 2021 Jan 23]. Available from: http://researchbank.rmit.edu.au/view/rmit:161426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alsharif A. Molecular and immunological characterisation of proteins from Anisakis pegreffii and their immune stimulatory effect on the human health system. [Thesis]. RMIT University; 2015. Available from: http://researchbank.rmit.edu.au/view/rmit:161426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. SHENOY RAJESH TULSIDAS. Structural Studies of Cysteine and Serine Protease Inhibitors Towards Therapeutic Applications.

Degree: 2009, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/29516

Subjects/Keywords: cysteine protease; serine protease; subtilisin; cathepsin; crystal structure; serine protease inhibitor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

TULSIDAS, S. R. (2009). Structural Studies of Cysteine and Serine Protease Inhibitors Towards Therapeutic Applications. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/29516

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

TULSIDAS, SHENOY RAJESH. “Structural Studies of Cysteine and Serine Protease Inhibitors Towards Therapeutic Applications.” 2009. Thesis, National University of Singapore. Accessed January 23, 2021. http://scholarbank.nus.edu.sg/handle/10635/29516.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

TULSIDAS, SHENOY RAJESH. “Structural Studies of Cysteine and Serine Protease Inhibitors Towards Therapeutic Applications.” 2009. Web. 23 Jan 2021.

Vancouver:

TULSIDAS SR. Structural Studies of Cysteine and Serine Protease Inhibitors Towards Therapeutic Applications. [Internet] [Thesis]. National University of Singapore; 2009. [cited 2021 Jan 23]. Available from: http://scholarbank.nus.edu.sg/handle/10635/29516.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

TULSIDAS SR. Structural Studies of Cysteine and Serine Protease Inhibitors Towards Therapeutic Applications. [Thesis]. National University of Singapore; 2009. Available from: http://scholarbank.nus.edu.sg/handle/10635/29516

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Ovat, Asli. Design, synthesis and evaluation of cysteine protease inhibitors.

Degree: PhD, Chemistry and Biochemistry, 2009, Georgia Tech

URL: http://hdl.handle.net/1853/33822

► Cysteine proteases are important drug targets due to their involvement in many biological processes such as protein turnover, digestion, blood coagulation, apoptosis, cell differentiation, cell… (more)

▼ Cysteine proteases are important drug targets due to their involvement in many biological processes such as protein turnover, digestion, blood coagulation, apoptosis, cell differentiation, cell signaling, and the immune response. In this thesis, we have reported the design, synthesis and evaluation of clan CA and clan CD cysteine protease inhibitors. Aza-peptidyl Michael acceptor and epoxide inhibitors for asparaginyl endopeptidases (legumains) from the bloodfluke, Schistosoma mansoni (SmAE) and the hard tick, Ixodes ricinus (IrAE) were designed and synthesized. SARs were similar, but with some notable exceptions. Both enzymes prefer disubstituted amides to monosubstituted amides in the P1' position and potency increased as we increased the hydrophobicity of the inhibitor in this position. Extending the inhibitor to P5 resulted in increased inhibitory potency, especially against IrAE, and both enzymes prefer small over large hydrophobic residues in the P2 position. Aza-peptide Michael acceptor inhibitors are more potent than aza-peptide epoxide inhibitors and, for some of these compounds, second order inhibition rate constants are the fastest yet discovered. We have also synthesized aza-peptidyl Michael acceptor and epoxide inhibitors for the parasitic cysteine proteases; cruzain, rhodesain. We have found that monosubstituted amides were favored over disubstituted amides indicating the involvement of the amide hydrogen in a H-bond network. We have shown that aza-peptide epoxides were as potent as Michael acceptors and we have obtained compounds with IC50 values as low as 20 nM. We have worked on the synthesis of heterocyclic peptidyl α-ketoamides, peptidyl ketones and aza-peptidyl ketones as calpain inhibitors. We have synthesized peptidyl α-ketoamides with nucleotide bases in the primed region to create compounds that can cross the blood-brain barrier. We have improved the potency by introducing a hydrophobic group on the adenine ring. We have obtained compounds with Ki values in the nanomolar range. We have designed peptidyl aminoketones as a new class of inhibitors for calpain. Peptidyl aminoketones were less potent than peptidyl α-ketoamides but still reasonable inhibitors of calpain that have the potential to cross the BBB. Advisors/Committee Members: Powers, James C. (Committee Chair), Christoph J. Fahrni (Committee Member), Jonathan D. Glass (Committee Member), Nicholas V. Hud (Committee Member), Sheldon W. May (Committee Member).

Subjects/Keywords: Legumain; Calpain; Protease; Cysteine; Cysteine proteinases; Cysteine proteinases Inhibitors; Protease inhibitors; Biosynthesis; Epoxy compounds

…specific substrate binding sites of the protease. CYSTEINE PROTEASES Cysteine proteases have a… …structures to papain. Papain is a cysteine protease extracted from the tropical papaya fruit. It is… …the first cysteine protease to be discovered and has been the subject of mechanism and… …lysosomal cysteine protease with both endopeptidase and dipeptidyl carboxypeptidase activities… …diseases.13 Cruzain is the only parasitic cysteine protease with a crystal structure.14-16…

6 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ovat, A. (2009). Design, synthesis and evaluation of cysteine protease inhibitors. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/33822

Chicago Manual of Style (16^th Edition):

Ovat, Asli. “Design, synthesis and evaluation of cysteine protease inhibitors.” 2009. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/33822.

MLA Handbook (7^th Edition):

Ovat, Asli. “Design, synthesis and evaluation of cysteine protease inhibitors.” 2009. Web. 23 Jan 2021.

Vancouver:

Ovat A. Design, synthesis and evaluation of cysteine protease inhibitors. [Internet] [Doctoral dissertation]. Georgia Tech; 2009. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/33822.

Council of Science Editors:

Ovat A. Design, synthesis and evaluation of cysteine protease inhibitors. [Doctoral Dissertation]. Georgia Tech; 2009. Available from: http://hdl.handle.net/1853/33822

23. Leite, Ney Ribeiro. Estudos moleculares de duas triptofanil tRNA sintetases do parasita Leishmania major e de uma cisteíno protease da bactéria Xylella fastidiosa.

Degree: PhD, Física Aplicada, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-08092007-163023/ ;

►

As aminoacil tRNA sintetases (AaRSs) são enzimas essenciais na síntese de proteínas assegurando a correta relação entre os aminoácidos e seus tRNA cognatos. O genoma… (more)

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As aminoacil tRNA sintetases (AaRSs) são enzimas essenciais na síntese de proteínas assegurando a correta relação entre os aminoácidos e seus tRNA cognatos. O genoma mitocondrial dos tripanossomatídeos perdeu os genes codificantes dos tRNAs, assim os tRNA mitocondriais são codificados no núcleo e importados do citoplasma. O código genético do kinetoplasto desvia do código genético pela utilização do códon de terminação UGA para a decodificação do códon do triptofano. Um único gene codificando o tRNATrp(CCA) observado no genoma de Leismania é responsável pela incorporação do aminoácido triptofano durante a síntese proteíca na mitocôndria. Para decodificar os dois códons do Trp (UGA e UGG) a base na posição 34 do tRNATrp(CCA) passa por um evento de editoração, convertendo o ribunuclotídeo C34 em U34, produzindo o tRNATrp(UCA) capaz de decodificar o códon UGA. Nesse trabalho foram caracterizadas duas triptofanil tRNA sintetases de Leishmania major. De acordo com experimentos de ?western blotting? e análises ?in silico? das seqüências de aminoácidos, uma enzima tem localização citoplasmática (LmTrpRS1) enquanto a outra mitocondrial (LmTrpRS2). Os mRNAs dos dois genes foram definidos por experimentos de 5? e 3? RT-PCR. As duas enzimas foram clonadas em diversos vetores de expressão procariotos e eucariotos. A LmTrpRS1 foi obtida somente na fração insolúvel, já a LmTrpRS2 foi obtida na fração solúvel quando clonada no vetor de expressão pET28a. Esta porém mostrou-se instável precipitando rapidamente após sua purificação. Os ensaios enzimáticos realizados com a mesma mostraram que ela é capaz de reconhecer os tRNAsTrp editado e não editado. Modelagem molecular por homologia com as duas proteínas foi realizada usando a proteína citoplasmática humana como molde, para estudar a interação entre a proteína e o tRNATrp. Xylella fastidiosa é um bactéria gram negativa limitada ao xilema, responsável por um grande número de doenças economicamente importantes, como a doença de Pierces em videiras, Clorose variegata do Citrus (CVC) e a doença da requeima das folhas em outras plantas incluindo, amendoeira, ameixeira, louro, amoreira e café. Em todos os casos a X. fastidiosa afeta o xylema da planta causando redução na produção de frutos. Nesse trabalho nós mostramos a estrutura da Xylellaína, uma cisteíno protease desse patógeno. A estrutura foi resolvida por dispersão anômala a um único comprimento de onda, utilizando cristais de xylellaína selenometionina substituídos. A estrutura da Xylellaína foi refinada até 1,65 Å de resolução, mostrando enovelamento similar às proteínas da família da papaína, porém algumas características interessantes como uma região N-terminal composta por 38 aminoácidos cobrindo o sulco ativo da enzima, um intrigante ribonucleotídeo encontrado fora do sítio ativo da enzima e um ?loop? semelhante ao ?loop? de oclusão presente na catepsina B.

The aminoacyl tRNA synthetases (aaRSs) are essential enzymes in protein synthesis that ensure the correct match between amino acids and their cognate tRNAs. The…

Advisors/Committee Members: Thiemann, Otavio Henrique.

Subjects/Keywords: Amino acil tRNA synthetase; Aminoacil tRNA sintetases; Cisteíno proteases; crystal structure; cysteine protease; Estrutura tridimensional; protein; Proteínas; Xylella fastidiosa; Xylella fastidiosa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leite, N. R. (2007). Estudos moleculares de duas triptofanil tRNA sintetases do parasita Leishmania major e de uma cisteíno protease da bactéria Xylella fastidiosa. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/76/76132/tde-08092007-163023/ ;

Chicago Manual of Style (16^th Edition):

Leite, Ney Ribeiro. “Estudos moleculares de duas triptofanil tRNA sintetases do parasita Leishmania major e de uma cisteíno protease da bactéria Xylella fastidiosa.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 23, 2021. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-08092007-163023/ ;.

MLA Handbook (7^th Edition):

Leite, Ney Ribeiro. “Estudos moleculares de duas triptofanil tRNA sintetases do parasita Leishmania major e de uma cisteíno protease da bactéria Xylella fastidiosa.” 2007. Web. 23 Jan 2021.

Vancouver:

Leite NR. Estudos moleculares de duas triptofanil tRNA sintetases do parasita Leishmania major e de uma cisteíno protease da bactéria Xylella fastidiosa. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 23]. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-08092007-163023/ ;.

Council of Science Editors:

Leite NR. Estudos moleculares de duas triptofanil tRNA sintetases do parasita Leishmania major e de uma cisteíno protease da bactéria Xylella fastidiosa. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/76/76132/tde-08092007-163023/ ;

24. Júnia de Oliveira Costa. Caracterização funcional e estrutural de novas proteases isoladas da peçonha de Bothrops alternatus e do látex de Euphorbia milii var. hislopii.

Degree: 2010, Federal University of Uberlândia

URL: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3128

► CAPÍTULO II: Uma serinoprotease da peçonha de Bothrops alternatus foi isolada por meio de três etapas cromatográficas: DEAE Sephacel, Sephadex G-75 e Benzamidina-Sepharose. A enzima… (more)

▼ CAPÍTULO II: Uma serinoprotease da peçonha de Bothrops alternatus foi isolada por meio de três etapas cromatográficas: DEAE Sephacel, Sephadex G-75 e Benzamidina-Sepharose. A enzima purificada, denominada balternina, apresentou uma única banda protéica quando analisada em SDS-PAGE, eletroforese em gel de poliacrilamida sob condições desnaturantes, apresentando peso molecular de aproximadamente 31.500 e 27.000 na presença e na ausência de agente redutor, respectivamente. O cDNA completo foi obtido por RT-PCR e a proteína madura de 236 resíduos de aminoácidos foi codificada por 708 pares de bases. O alinhamento múltiplo das sequências de aminoácidos deduzidas desta enzima mostrou similaridade estrutural com outras serinoproteases de peçonhas de serpentes. A balternina apresentou atividades fibrinogenolítica e albuminolítica. Quando a protease e o fibrinogênio bovino foram incubados a 37 C, na proporção de 1:10 (m/m), a enzima clivou preferencialmente a cadeia Aα e, aparentemente, não degradou as cadeias Bβ e do substrato. Testes de estabilidade mostraram que o intervalo ótimo de temperatura e pH para a atividade proteolítica sobre o fibrinogênio foram de 30 a 40 C e 7,0 a 8,0, respectivamente. O efeito de inibidores enzimáticos mostrou que a benzamidina inibiu a atividade fibrinogenolítica, indicando que a balternina é uma serinoprotease. A enzima isolada causou, ainda, alterações morfológicas no coração, fígado, pulmão e músculo de camundongos, desencadeando também coagulação in vitro e desfibrinogenação in vivo, quando administrada intraperitonealmente em camundongos, sugerindo ser uma enzima thrombin-like. Desta forma, a balternina pode ser de interesse como agente terapêutico na prevenção e tratamento de desordens trombóticas. CAPÍTULO III: A protease, designada de eumilina, foi isolada do látex de Euphorbia milii var. hislopii pela combinação de duas etapas cromatográficas: troca iônica (DEAE-Sephacel) e filtração em gel (Sephadex G-75). A eumilina é uma proteína monomérica com massa molecular aparente de 30 kDa quando analisada em SDS-PAGE sob condições redutoras e apresentou um pico principal de 29,814 kDa em espectrometria de massa MALDI-TOF/TOF. A eumilina apresentou atividades caseinolítica e fibrinogenolítica, mas não desencadeou atividade hemorrágica ou desfibrinogenante. Esta protease hidrolisa preferencialmente a cadeia A do fibrinogênio e, mais lentamente, a cadeia B. A atividade fibrinogenolítica foi inibida por b-mercaptoetanol e leupeptina. Em contraste, o EDTA e a benzamidina não afetaram a referida atividade. A ação proteolítica da eumilina sobre a caseína apresentou pH ótimo igual a 8,0 e foi estável em solução até 40 C; perdendo completamente a atividade em temperatura 80 C. A injeção Intraplantar de eumilina (125 μg/pata) causou hiperalgesia dose e tempodependente, com pico entre 1 a 5 h após injeção de enzima. Da mesma forma, causou resposta edematogênica máxima após 1 h. A análise morfológica indicou que a eumilina induziu mionecrose intensa, com infiltrado leucocitário e danos… Advisors/Committee Members: Nilson Penha Silva, Andreimar Martins Soares, Luiz Fernando Moreira Izidoro, Fábio de Oliveira, Guilherme Rocha Lino de Souza, Márcia Helena Borges, Silvana Marcussi.

Subjects/Keywords: GENETICA; Cobra venenosa - Veneno; Bothrops; Peçonha de serpente; Bothrops alternatus; Euphorbiaceae; Crown-of-Thorns; Desfibrinogenação; Fibrinogenase; Euphorbia milii; Cysteine protease; Eumiliin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Costa, J. d. O. (2010). Caracterização funcional e estrutural de novas proteases isoladas da peçonha de Bothrops alternatus e do látex de Euphorbia milii var. hislopii. (Thesis). Federal University of Uberlândia. Retrieved from http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3128

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Costa, Júnia de Oliveira. “Caracterização funcional e estrutural de novas proteases isoladas da peçonha de Bothrops alternatus e do látex de Euphorbia milii var. hislopii.” 2010. Thesis, Federal University of Uberlândia. Accessed January 23, 2021. http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3128.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Costa, Júnia de Oliveira. “Caracterização funcional e estrutural de novas proteases isoladas da peçonha de Bothrops alternatus e do látex de Euphorbia milii var. hislopii.” 2010. Web. 23 Jan 2021.

Vancouver:

Costa JdO. Caracterização funcional e estrutural de novas proteases isoladas da peçonha de Bothrops alternatus e do látex de Euphorbia milii var. hislopii. [Internet] [Thesis]. Federal University of Uberlândia; 2010. [cited 2021 Jan 23]. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3128.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Costa JdO. Caracterização funcional e estrutural de novas proteases isoladas da peçonha de Bothrops alternatus e do látex de Euphorbia milii var. hislopii. [Thesis]. Federal University of Uberlândia; 2010. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3128

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

25. Chapeton Montes, Diana Joanne. Influence de l'environnement sur le protéome de surface de Clostridium difficile : analyse globale et caractérisation de la cystéine protéase Cwp84 : Influence of environment on the surface proteome of Clostridium difficile : global analysis and characterization of the cysteine protease Cwp84.

Degree: Docteur es, Microbiologie et thérapeutiques anti-infectieuses, 2012, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2012PA114809

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Clostridium difficile est une bactérie pathogène responsable de diarrhées nosocomiales et de la plupart des colites pseudomembraneuses. Le principal facteur de risque est la prise… (more)

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Clostridium difficile est une bactérie pathogène responsable de diarrhées nosocomiales et de la plupart des colites pseudomembraneuses. Le principal facteur de risque est la prise d’antibiotiques qui altère la composition du microbiote intestinal, et favorise ainsi l’implantation de la bactérie au niveau colique. Après une étape de colonisation, la bactérie produit ses principaux facteurs de virulence, les toxines A et B. La colonisation est un processus multifactoriel, qui met en jeu différentes protéines de surface dont des adhésines et une cystéine protéase Cwp84.Dans une première partie, nous avons analysé le processus de maturation de la protéase Cwp84, ainsi que sa localisation dans la bactérie, afin de mieux comprendre son rôle dans la virulence de C. difficile. La protéase recombinante, purifiée sous forme de zymogène, présente un processus de maturation particulier comprenant des clivages successifs, qui aboutissent à la forme mature de 47 KDa. La protéase ainsi activée présente une activité protéolytique sur la fibronectine. Dans la bactérie, Cwp84 existe sous deux formes majoritaires, associées à la surface de la bactérie : une première forme, d’environ 80 KDa, associée aux protéines de la couche S, dont le rôle serait de cliver le précurseur des protéines de la couche S en deux protéines matures ; une deuxième forme, d’environ 50 KDa correspondant vraisemblablement à la forme mature de la protéase recombinante de 47 KDa, est retrouvée à la fois dans la fraction extracellulaire et associée à la surface de la bactérie. Nous avons montré que la protéase rélarguée est capable de se ré-associer sous sa forme mature de manière spécifique à la surface de C. difficile. Dans une deuxième partie, nous avons analysé l’impact de conditions environnementales mimant celles rencontrées par la bactérie au cours de son transit dans le tractus digestif de l’hôte, sur la modulation de facteurs de colonisation, dont la protéase. Nous avons montré qu’un pH acide favorise à la fois l’expression et le processus de maturation de la protéase vers sa forme mature de 47 KDa. Des analyses protéomiques et transcriptomiques ont montré que d’autres protéines impliquées dans colonisation sont surexprimées dans un milieu avec glucose, cette régulation étant vraisemblablement liée à la diminution du pH résultant de la fermentation du glucose plutôt qu’à un effet direct de ce sucre. Cette régulation des facteurs de virulence par le pH acide est probablement un élément favorable au processus de colonisation de l’hôte. Ces différentes analyses ont également permis l’identification de facteurs de virulence potentiels, qui devront être caractérisés par la suite.

Clostridium difficile, a gram-positive spore-forming, anaerobic bacterium, is the etiological agent of pseudomembranous colitis and of many cases of nosocomial diarrhea. The main risk factor is the use of antibiotics that alters the intestinal microbiota, predisposing to C. difficile intestinal colonization. C. difficile pathogenicity is mediated mainly by its A and B toxins,…

Advisors/Committee Members: Janoir, Claire (thesis director).

Subjects/Keywords: Cystéine protéase Cwp84; Processus de maturation; Régulation; Facteurs de virulence; Clostridium difficile; Cysteine protease Cwp84; Maturation process; PH; Regulation; Virulence factors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chapeton Montes, D. J. (2012). Influence de l'environnement sur le protéome de surface de Clostridium difficile : analyse globale et caractérisation de la cystéine protéase Cwp84 : Influence of environment on the surface proteome of Clostridium difficile : global analysis and characterization of the cysteine protease Cwp84. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA114809

Chicago Manual of Style (16^th Edition):

Chapeton Montes, Diana Joanne. “Influence de l'environnement sur le protéome de surface de Clostridium difficile : analyse globale et caractérisation de la cystéine protéase Cwp84 : Influence of environment on the surface proteome of Clostridium difficile : global analysis and characterization of the cysteine protease Cwp84.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 23, 2021. http://www.theses.fr/2012PA114809.

MLA Handbook (7^th Edition):

Chapeton Montes, Diana Joanne. “Influence de l'environnement sur le protéome de surface de Clostridium difficile : analyse globale et caractérisation de la cystéine protéase Cwp84 : Influence of environment on the surface proteome of Clostridium difficile : global analysis and characterization of the cysteine protease Cwp84.” 2012. Web. 23 Jan 2021.

Vancouver:

Chapeton Montes DJ. Influence de l'environnement sur le protéome de surface de Clostridium difficile : analyse globale et caractérisation de la cystéine protéase Cwp84 : Influence of environment on the surface proteome of Clostridium difficile : global analysis and characterization of the cysteine protease Cwp84. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2012PA114809.

Council of Science Editors:

Chapeton Montes DJ. Influence de l'environnement sur le protéome de surface de Clostridium difficile : analyse globale et caractérisation de la cystéine protéase Cwp84 : Influence of environment on the surface proteome of Clostridium difficile : global analysis and characterization of the cysteine protease Cwp84. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA114809

University of California – San Francisco

26. O'Brien, Theresa Cunningham. Cysteine Protease Function in Trypanosoma brucei.

Degree: Biochemistry and Molecular Biology, 2007, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/2m09z6f0

► Treatment of Trypanosoma brucei with cysteine protease inhibitors demonstrated that clan CA cysteine protease activity was essential for parasite viability in culture and infection in… (more)

▼ Treatment of Trypanosoma brucei with cysteine protease inhibitors demonstrated that clan CA cysteine protease activity was essential for parasite viability in culture and infection in mice. However, the identity and biological function of the essential protease or proteases targeted by these inhibitors was not known. Biochemical, molecular biology, and proteomics techniques were used to identify potential targets of inhibition and to investigate the biological function of cysteine proteases in T. brucei. A search of the T. brucei genome revealed two cysteine proteases: the previously characterized cathepsin L-like cysteine protease, rhodesain, and a newly-identified cathepsin B-like cysteine protease, tbcatB. Rhodesain is expressed at high levels in procyclic and bloodstream form parasites. TbcatB was found to be expressed at low levels relative to rhodesain, but was more abundant in bloodstream form parasites. RNA interference (RNAi) of rhodesain in cultured parasites did not produce a phenotype. In contrast, a modest decrease in tbcatB mRNA and protein caused by RNAi produced a marked phenotype, with swollen endosomes, accumulation of fluorescein isothiocyanate-transferrin, and a block in cytokinesis, resulting in death. Induction of RNAi against tbcatB in a mouse model of infection confirmed that tbcatB is essential for parasite survival in vivo. The in vivo induction of RNAi against rhodesain did not cure infection, although it extended the survival of five out of ten mice beyond 60 days post-infection. TbcatB-deficiency produced by either TBCATB heterozygosity or RNAi caused morphological abnormalities similar to those seen with cysteine protease inhibitors. TbcatB-deficient parasites exhibited swollen flagellar pockets and accumulated unprocessed or undegraded host and parasite proteins, including transferrin, p67, and rhodesain. Expression and biochemical purification of active, recombinant tbcatB from Pichia pastoris allowed for the determination of the P1-P4 substrate specificity profile. Both results of transferrin cleavage assays and predictions of host substrates based on substrate specificity support the hypothesis that a key function for tbcatB is the degradation of host transferrin. Transferrin receptor expression is specifically upregulated in tbcatB-deficient parasites, consistent with iron starvation. TbcatB is therefore critical to the survival of T. brucei and a logical drug target for the development of new anti-trypanosomal chemotherapy.

Subjects/Keywords: Chemistry, Biochemistry; Biology, Parasitology; Trypanosoma brucei; cysteine protease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

O'Brien, T. C. (2007). Cysteine Protease Function in Trypanosoma brucei. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/2m09z6f0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

O'Brien, Theresa Cunningham. “Cysteine Protease Function in Trypanosoma brucei.” 2007. Thesis, University of California – San Francisco. Accessed January 23, 2021. http://www.escholarship.org/uc/item/2m09z6f0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

O'Brien, Theresa Cunningham. “Cysteine Protease Function in Trypanosoma brucei.” 2007. Web. 23 Jan 2021.

Vancouver:

O'Brien TC. Cysteine Protease Function in Trypanosoma brucei. [Internet] [Thesis]. University of California – San Francisco; 2007. [cited 2021 Jan 23]. Available from: http://www.escholarship.org/uc/item/2m09z6f0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

O'Brien TC. Cysteine Protease Function in Trypanosoma brucei. [Thesis]. University of California – San Francisco; 2007. Available from: http://www.escholarship.org/uc/item/2m09z6f0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. LIM JUN JI. IDENTIFICATION AND CHARACTERIZATION OF A PLASMODIUM VIVAX INHIBITOR OF CYSTEINE PROTEASES.

Degree: 2013, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/49264

Subjects/Keywords: Plasmodium vivax; cysteine protease inhibitors; vivapains haemoglobinase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

JI, L. J. (2013). IDENTIFICATION AND CHARACTERIZATION OF A PLASMODIUM VIVAX INHIBITOR OF CYSTEINE PROTEASES. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/49264

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

JI, LIM JUN. “IDENTIFICATION AND CHARACTERIZATION OF A PLASMODIUM VIVAX INHIBITOR OF CYSTEINE PROTEASES.” 2013. Thesis, National University of Singapore. Accessed January 23, 2021. http://scholarbank.nus.edu.sg/handle/10635/49264.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

JI, LIM JUN. “IDENTIFICATION AND CHARACTERIZATION OF A PLASMODIUM VIVAX INHIBITOR OF CYSTEINE PROTEASES.” 2013. Web. 23 Jan 2021.

Vancouver:

JI LJ. IDENTIFICATION AND CHARACTERIZATION OF A PLASMODIUM VIVAX INHIBITOR OF CYSTEINE PROTEASES. [Internet] [Thesis]. National University of Singapore; 2013. [cited 2021 Jan 23]. Available from: http://scholarbank.nus.edu.sg/handle/10635/49264.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

JI LJ. IDENTIFICATION AND CHARACTERIZATION OF A PLASMODIUM VIVAX INHIBITOR OF CYSTEINE PROTEASES. [Thesis]. National University of Singapore; 2013. Available from: http://scholarbank.nus.edu.sg/handle/10635/49264

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Shah, Falgun. Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection.

Degree: PhD, Pharmaceutical Sciences, 2011, University of Mississippi

URL: https://egrove.olemiss.edu/etd/260

► A wide array of molecular modeling tools were utilized to design and develop inhibitors against cysteine protease of P. Falciparum Malaria and Severe Acute Respiratory… (more)

▼ A wide array of molecular modeling tools were utilized to design and develop inhibitors against cysteine protease of P. Falciparum Malaria and Severe Acute Respiratory Syndrome (SARS). A number of potent inhibitors were developed against cysteine protease and hemoglobinase of P. falciparum , referred as Falcipains (FPs), by the structure-based virtual screening of the focused libraries enriched in soft-electrophiles containing compounds. Twenty one diverse, non-peptidic, low micromolar hits were identified. A combined data mining and combinatorial library synthesis approach was performed to discover analogs of virtual screening hits and establish the structure-activity relationships (SAR). However, the resulting SAR of the identified hits was unusually steep in some cases and could not be explained by a traditional analysis of the interactions (electrostatics, van der Waals or H-bond). To gain insights, a statistical thermodynamic analysis of explicit solvent in the ligand binding domain of FP-2 and FP-3 was performed that explained some of the complex trends in the SAR. Furthermore, the moderate potency of a subset of FP-2 hits was elucidated using quantum mechanics calculations that showed reduced reactivity of the electrophilic center of these hits. In addition, solvent thermodynamics and reactivity analysis also helped to elucidate the complex trends in SAR of peptidomimetic inhibitors of FP-2 and FP-3 synthesized in our laboratory. Multi nanosecond explicit solvent molecular dynamics simulations were carried out using the docking poses of the known inhibitors in the binding site of SARS-3CLpro, a cysteine protease important for replication of SARS virus, to study the overall stability of the binding site interactions as well as identify important changes in the interaction profile that were not apparent from the docking study. Analysis of the simulation studies led to the identification of certain protein-ligand interaction patterns which would be useful in further structure based design efforts against cysteine protease (3CLpro) of SARS. Advisors/Committee Members: Stephen J. Cutler, Robert J. Doerksen, Babu L. Tekwani.

Subjects/Keywords: Chemical Reactivity; Cysteine Protease; Falcipain; Solvent Thermodynamics; Structure-Based Drug Design; Virtual Screening; Pharmacy and Pharmaceutical Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shah, F. (2011). Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection. (Doctoral Dissertation). University of Mississippi. Retrieved from https://egrove.olemiss.edu/etd/260

Chicago Manual of Style (16^th Edition):

Shah, Falgun. “Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection.” 2011. Doctoral Dissertation, University of Mississippi. Accessed January 23, 2021. https://egrove.olemiss.edu/etd/260.

MLA Handbook (7^th Edition):

Shah, Falgun. “Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection.” 2011. Web. 23 Jan 2021.

Vancouver:

Shah F. Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection. [Internet] [Doctoral dissertation]. University of Mississippi; 2011. [cited 2021 Jan 23]. Available from: https://egrove.olemiss.edu/etd/260.

Council of Science Editors:

Shah F. Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection. [Doctoral Dissertation]. University of Mississippi; 2011. Available from: https://egrove.olemiss.edu/etd/260

29. Groeme, Rachel. Production et caractérisation structurale et fonctionnelle d’un nouvel allergène majeur du pollen d’Ambroisie : la protéase à cystéine Amb a 11 : Production and structural and functionnal characterization of a new major allergen from short ragweed pollen : the cysteine protease Amb a 11.

Degree: Docteur es, Sciences de la vie et de la santé, 2015, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2015SACLA006

►

Le projet de thèse à pour but de produire et caractériser un nouvel allergène de pollen d'ambroisie. Le projet est décliné en cinq axes: production… (more)

Subjects/Keywords: Allergène; Protéase à cystéine; Ambroisie; Structure-Fonction; Immunothérapie; Allergen; Cysteine protease; Short Ragweed; Structure-Function; Immunotherapy; 616.202

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Groeme, R. (2015). Production et caractérisation structurale et fonctionnelle d’un nouvel allergène majeur du pollen d’Ambroisie : la protéase à cystéine Amb a 11 : Production and structural and functionnal characterization of a new major allergen from short ragweed pollen : the cysteine protease Amb a 11. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2015SACLA006

Chicago Manual of Style (16^th Edition):

Groeme, Rachel. “Production et caractérisation structurale et fonctionnelle d’un nouvel allergène majeur du pollen d’Ambroisie : la protéase à cystéine Amb a 11 : Production and structural and functionnal characterization of a new major allergen from short ragweed pollen : the cysteine protease Amb a 11.” 2015. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed January 23, 2021. http://www.theses.fr/2015SACLA006.

MLA Handbook (7^th Edition):

Groeme, Rachel. “Production et caractérisation structurale et fonctionnelle d’un nouvel allergène majeur du pollen d’Ambroisie : la protéase à cystéine Amb a 11 : Production and structural and functionnal characterization of a new major allergen from short ragweed pollen : the cysteine protease Amb a 11.” 2015. Web. 23 Jan 2021.

Vancouver:

Groeme R. Production et caractérisation structurale et fonctionnelle d’un nouvel allergène majeur du pollen d’Ambroisie : la protéase à cystéine Amb a 11 : Production and structural and functionnal characterization of a new major allergen from short ragweed pollen : the cysteine protease Amb a 11. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2015. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2015SACLA006.

Council of Science Editors:

Groeme R. Production et caractérisation structurale et fonctionnelle d’un nouvel allergène majeur du pollen d’Ambroisie : la protéase à cystéine Amb a 11 : Production and structural and functionnal characterization of a new major allergen from short ragweed pollen : the cysteine protease Amb a 11. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2015. Available from: http://www.theses.fr/2015SACLA006

Virginia Tech

30. Petzold, Herman Earl III. Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana.

Degree: MS, Horticulture, 2007, Virginia Tech

URL: http://hdl.handle.net/10919/32582

► The process of xylem tracheary element differentiation involves the coordination of vascular cambium activity, cell fate determination, cell expansion/elongation, secondary wall synthesis, programmed cell death,… (more)

Subjects/Keywords: tracheary element; Arabidopsis thaliana; cysteine protease; promoter deletion; xylem

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Petzold, H. E. I. (2007). Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/32582

Chicago Manual of Style (16^th Edition):

Petzold, Herman Earl III. “Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana.” 2007. Masters Thesis, Virginia Tech. Accessed January 23, 2021. http://hdl.handle.net/10919/32582.

MLA Handbook (7^th Edition):

Petzold, Herman Earl III. “Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana.” 2007. Web. 23 Jan 2021.

Vancouver:

Petzold HEI. Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana. [Internet] [Masters thesis]. Virginia Tech; 2007. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10919/32582.

Council of Science Editors:

Petzold HEI. Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana. [Masters Thesis]. Virginia Tech; 2007. Available from: http://hdl.handle.net/10919/32582

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Open Access Theses and Dissertations