subject:(Cultured cells)

University of Utah

1. Wallace, Lane Jackson. Adenosine 3',5'-monophosphate in highly purified and mixed primary cultures of neurons and non-neuronal cells from embryonic chick sympathetic ganglia;.

Degree: PhD, Pharmacology & Toxicology;, 1997, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/113/rec/9

► Primary cultures containing 99% neurons, 99% non-neuronal cell (glia), or both cell types prepared from the sympathetic ganglia of 12-day chick embryos. Levels of cyclic… (more)

▼ Primary cultures containing 99% neurons, 99% non-neuronal cell (glia), or both cell types prepared from the sympathetic ganglia of 12-day chick embryos. Levels of cyclic AMP in the non-neuronal cells (~ 14 pmoles per mg protein) were approximately three-fold higher than levels in the neurons (~ 4 pmoles per mg protein). Mixed cultures had concentrations of cyclic AMP which fell between the values measured for pure neuronal and pure non-neuronal cultures. The measured cyclic AMP values of mixed cultures were indistinguishable from values predicted by summing the expected contributions of the neurons and non-neuronal cells. Thus, contact between the neurons and non-neuronal cells in these mixed cultures did not appear to alter the level of cyclic AMP in either cell type. Neuronal-glial interactions, such as the specific neuronal stimulation of non-neuronal cell proliferation, occurred independently of any changes in the level of cyclic AMP in the mixed cultures. Cell density was varied in both pure and mixed cultures, and both cyclic AMP concentrations and amounts of 3H-thymidine incorporation into DNA were measured. The cyclic AMP content of the non-neuronal cells varied inversely with cell density. 3H-Thymidine incorporation was independed of cell density in both neuronal and non-neuronal cultures. Parallel density-dependent decreases in cyclic AMP concentration and 3H-thymidine incorporation were observed in mixed cultures as cell density was increased. The data suggest that there is no relationship between changes in rate of non-neuronal cell proliferation and cyclic AMP levels in these cultures. The effects of some putative neurotransmitters, analogs of transmitters, and adenosine on levels of cyclic AMP were determined in primary neuronal, non-neural, and mixed cultures of embryonic chick sympathetic ganglion cells. Adenosine increased the level of cyclic AMP in the non-neuronal cells but had no effect on levels in either neuronal or mixed cultures. Norepinephrine increased the level of cyclic AMP in neurons, had no effect on the non-neuronal levels, and decreased the cyclic nucleotide content of mixed cultures. Dopamine, isoproterenol, and pilocarpine all caused a reduction in the cyclic AMP content of mixed cultures but had no effect on levels of either neurons on non-neuronal cells. The measured cyclic AMP levels of norepinephrine- and isoproterenol-treated mixed cultures were significantly lower than the values predicted by summing the expected individual contributions of the neurons and the non-neuronal cells. Thus, cells in mixed cultures sometimes responded differently from cells in pure cultures.

Subjects/Keywords: Pharmacology; Cells; Cultured

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APA (6^th Edition):

Wallace, L. J. (1997). Adenosine 3',5'-monophosphate in highly purified and mixed primary cultures of neurons and non-neuronal cells from embryonic chick sympathetic ganglia;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/113/rec/9

Chicago Manual of Style (16^th Edition):

Wallace, Lane Jackson. “Adenosine 3',5'-monophosphate in highly purified and mixed primary cultures of neurons and non-neuronal cells from embryonic chick sympathetic ganglia;.” 1997. Doctoral Dissertation, University of Utah. Accessed January 19, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/113/rec/9.

MLA Handbook (7^th Edition):

Wallace, Lane Jackson. “Adenosine 3',5'-monophosphate in highly purified and mixed primary cultures of neurons and non-neuronal cells from embryonic chick sympathetic ganglia;.” 1997. Web. 19 Jan 2021.

Vancouver:

Wallace LJ. Adenosine 3',5'-monophosphate in highly purified and mixed primary cultures of neurons and non-neuronal cells from embryonic chick sympathetic ganglia;. [Internet] [Doctoral dissertation]. University of Utah; 1997. [cited 2021 Jan 19]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/113/rec/9.

Council of Science Editors:

Wallace LJ. Adenosine 3',5'-monophosphate in highly purified and mixed primary cultures of neurons and non-neuronal cells from embryonic chick sympathetic ganglia;. [Doctoral Dissertation]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/113/rec/9

McGill University

2. Spain, Michael J. The effects of serum low density lipoproteins on skin fibroblasts and aortic smooth muscle cells in culture.

Degree: PhD, Department of Experimental Medicine, 1979, McGill University

URL: https://escholarship.mcgill.ca/downloads/ff365762s.pdf ; https://escholarship.mcgill.ca/concern/theses/xw42nb24b

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Des facteurs majeurs dans l'athérogènes semblent être les lipoprotéines plasmatiques.L'effet des lipoprotéines sériques à faible densité (LDL) d'origine humaine fut étudiés dans des cultures de… (more)

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Des facteurs majeurs dans l'athérogènes semblent être les lipoprotéines plasmatiques.L'effet des lipoprotéines sériques à faible densité (LDL) d'origine humaine fut étudiés dans des cultures de fibroblastesde peau humaines maintenues dans un milieu additioné de sérum humain pauvre en plaquettes et dépourvu de lipoprotéines (PPLFS). Les effets produits par les LDL furent une augmentation de la vitesse de réplication cellulaire, une augmentation du contenu cellulaire en cholestérol et en protéines, une augmentation de la grosseur des cellules et un accroissement de l'accumulation des glycosaminoglycanes (GAG) dans le milieu de culture. L'effet des LDL sur la prolifération cellulaire fut aboli par l'ultrafiltration du PPLFS. Les augmentations de cholestérol et de protéine étaient proportionnelles ainsi que les augmentations en protéines et en grosseur cellulaire. Nous avons déduit de ces résultats que les augmentations en cholestérol et en protéines étaient de type structural plutôt que d'emmagasinement. Le contenu protéique élevé de cellules est dû à une diminution de leur vitesse de dégradation. [...]

Plasma lipoproteins appear to be major factors in atherogenesis. The effects of human serum low density lipoproteins (LDL) were studied in cultures of human skin fibroblasts maintained in medium supplemented with human platelet-poor, lipoprotein-free serum· (PPLFS). LDL increased the proliferation, the size, and the contents of cholesterol and protein of cells, and led to an enhanced accumulation of glycosaminoglycans (GAG) in the medium. The LDL effect on proliferation was abolished by ultrafiltration of PPLFS. Increases in cholesterol and protein were proportional as were' increases in protein and cell size. It was inferred that cholesterol and protein accumulated in structural rather than storage forms. Elevations in cellular protein content were caused by a decreased rate of protein degradation. No LDL effect on proliferation was observed in cultures of porcine aortic smooth muscle cells in PPLFS medium. No abnormalities of diabetic cell responses to LDL or of the compositions of serum lipoproteins from diabetics were found.

Advisors/Committee Members: Dr. N. Kalant.

Subjects/Keywords: Lipoproteins.; Cells, Cultured.

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APA (6^th Edition):

Spain, M. J. (1979). The effects of serum low density lipoproteins on skin fibroblasts and aortic smooth muscle cells in culture. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/ff365762s.pdf ; https://escholarship.mcgill.ca/concern/theses/xw42nb24b

Chicago Manual of Style (16^th Edition):

Spain, Michael J. “The effects of serum low density lipoproteins on skin fibroblasts and aortic smooth muscle cells in culture.” 1979. Doctoral Dissertation, McGill University. Accessed January 19, 2021. https://escholarship.mcgill.ca/downloads/ff365762s.pdf ; https://escholarship.mcgill.ca/concern/theses/xw42nb24b.

MLA Handbook (7^th Edition):

Spain, Michael J. “The effects of serum low density lipoproteins on skin fibroblasts and aortic smooth muscle cells in culture.” 1979. Web. 19 Jan 2021.

Vancouver:

Spain MJ. The effects of serum low density lipoproteins on skin fibroblasts and aortic smooth muscle cells in culture. [Internet] [Doctoral dissertation]. McGill University; 1979. [cited 2021 Jan 19]. Available from: https://escholarship.mcgill.ca/downloads/ff365762s.pdf ; https://escholarship.mcgill.ca/concern/theses/xw42nb24b.

Council of Science Editors:

Spain MJ. The effects of serum low density lipoproteins on skin fibroblasts and aortic smooth muscle cells in culture. [Doctoral Dissertation]. McGill University; 1979. Available from: https://escholarship.mcgill.ca/downloads/ff365762s.pdf ; https://escholarship.mcgill.ca/concern/theses/xw42nb24b

University of Utah

3. Pallavicini, Maria Georgina. In vivo effects of ftorafur and fluorouracil on mammary tumors and small intestine in mice.

Degree: PhD, Pharmacology & Toxicology;, 1997, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1082/rec/629

► The in vivo effects of Ftorafur (FT) and 5-fluorouracil (FU) were evaluated at several different organizational levels within the same animal model. On a molar… (more)

▼ The in vivo effects of Ftorafur (FT) and 5-fluorouracil (FU) were evaluated at several different organizational levels within the same animal model. On a molar basis, FU was found to be two to three times more potent than FT with respect to growth inhibition of murine mammary adenocarcinomas. However, administration of FT produced less host toxicity at a dose level which resulted in similar antitumor activity. Drug-induced perturbations in cell cycle phase distributions were analyzed by the flow cytometer (FCM). Although FCM analysis is limited by lack of information on the extent of dead and dying cells, data obtained with both drugs were consistent with the possibility of S phase cytotoxicity and drug-induce G1/S block or delay. Changes in the age distribution of perturbed tumor cells, is detected by FCM analysis, correlated with 3H-TdR autoradiography and with 32P incorporation into DNA but not with 3H-deoxyuridine incorporation into DNA. Both drugs depressed 3H-deosyuridine incorporation into DNA; however, incorporation in both the tumor and small intestine returned to control levels earlier with FT than with an equimolar dose of FU. However, both drugs produced similar patters of 3H-deoxyuridine incorporation when FT was administered at three times the molar equivalent dose of FU. The results of these studies also suggest that deoxyuridine incorporation primarily reflects drug effects on thymidylate synthetase and not necessarily the overall rate of DNA synthesis. This study also involved the development of techniques for the dispersal of solid tumors and intestinal lining epithelium into single cell suspensions for FCM analysis. Tetraphenylboron (TPB) was utilized as an aid to solid tumor dispersal. TPB-dispersed tumors yield DNA distributions with minimal cell clumping and low levels of cellular debris. Fluorescent enzyme histochemistry with subsequent FCM analysis was utilized to separate dispersed epithelial cells of the small intestine into crypt and villus subpopulations. When fully optimized, the epithelial cell separation should offer a rapid method by which perturbations in the age distribution of crypt cells can easily be evaluated by FCM.

Subjects/Keywords: Intestine, Small; Cells, Cultured

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APA (6^th Edition):

Pallavicini, M. G. (1997). In vivo effects of ftorafur and fluorouracil on mammary tumors and small intestine in mice. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1082/rec/629

Chicago Manual of Style (16^th Edition):

Pallavicini, Maria Georgina. “In vivo effects of ftorafur and fluorouracil on mammary tumors and small intestine in mice.” 1997. Doctoral Dissertation, University of Utah. Accessed January 19, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1082/rec/629.

MLA Handbook (7^th Edition):

Pallavicini, Maria Georgina. “In vivo effects of ftorafur and fluorouracil on mammary tumors and small intestine in mice.” 1997. Web. 19 Jan 2021.

Vancouver:

Pallavicini MG. In vivo effects of ftorafur and fluorouracil on mammary tumors and small intestine in mice. [Internet] [Doctoral dissertation]. University of Utah; 1997. [cited 2021 Jan 19]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1082/rec/629.

Council of Science Editors:

Pallavicini MG. In vivo effects of ftorafur and fluorouracil on mammary tumors and small intestine in mice. [Doctoral Dissertation]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1082/rec/629

University of Alberta

4. Secretan, Charles Coleman. The role of cultured chondrocytes and mesenchymal stem cells in the repair of acute articular cartilage injuries.

Degree: PhD, Department of Surgery, 2010, University of Alberta

URL: https://era.library.ualberta.ca/files/r494vk77z

► Osteoarthritis (OA) is a disease that has significant individual, social, and economic impact worldwide. Although many etiologies lead to the eventual development of OA, one… (more)

▼ Osteoarthritis (OA) is a disease that has significant individual, social, and economic impact worldwide. Although many etiologies lead to the eventual development of OA, one potentially treatable cause is the acute articular cartilage (AC) injury. These injuries are common and have a poor inherent healing capacity, leading to the formation of OA. In an effort to repair AC injuries several treatment strategies have been developed but none have proven completely successful. Studies examining AC tissue-engineering strategies have suggested that those with the most potential for success involve the introduction of autogenous or allogenous cells to the site of injury. These strategies are designed to encourage creation of a matrix with the appropriate characteristics of normal AC. However, development of a completely successful repair method has proven difficult because the biomechanical properties of normal AC are not easy to replicate, a cell source with the appropriate functional characteristics has not been optimized, and the problem of effective incorporation of a repair construct into the host tissue remains unresolved. In an effort to more fully understand the cartilage repair process, this work first focused on the development and utilization of an in vitro human explant model of AC to study the ability of seeded human chondrocytes to integrate into an AC defect. Further work elucidated the gene expression patterns of cultured adult human chondrocytes and human mesenchymal stem cell (MSC)-derived chondrocytes. Results from this work determined that cultured human chondrocytes were able to adhere to articular cartilage defects in a viable in vitro explant model and produce a matrix containing collagen type II. However, further work with the in vitro expanded chondrocytes revealed that these cells have increased expression of collagen type I which promotes the formation of a less durable fibrocartilagenous tissue. This unfavorable expression persisted despite placing the chondrocytes in an environment favoring a chondrocytic phenotype. Further work with MSC-derived chondrocytes demonstrated a similar and unfavorable production of collagen type I. This work represented an important first step towards a treatment for acute AC lesions but it is clear that further work to optimize the culture microenvironment is still required.

Subjects/Keywords: joint injury; articular cartilage; cultured chondrocytes; mesenchymal stem cells

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APA (6^th Edition):

Secretan, C. C. (2010). The role of cultured chondrocytes and mesenchymal stem cells in the repair of acute articular cartilage injuries. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/r494vk77z

Chicago Manual of Style (16^th Edition):

Secretan, Charles Coleman. “The role of cultured chondrocytes and mesenchymal stem cells in the repair of acute articular cartilage injuries.” 2010. Doctoral Dissertation, University of Alberta. Accessed January 19, 2021. https://era.library.ualberta.ca/files/r494vk77z.

MLA Handbook (7^th Edition):

Secretan, Charles Coleman. “The role of cultured chondrocytes and mesenchymal stem cells in the repair of acute articular cartilage injuries.” 2010. Web. 19 Jan 2021.

Vancouver:

Secretan CC. The role of cultured chondrocytes and mesenchymal stem cells in the repair of acute articular cartilage injuries. [Internet] [Doctoral dissertation]. University of Alberta; 2010. [cited 2021 Jan 19]. Available from: https://era.library.ualberta.ca/files/r494vk77z.

Council of Science Editors:

Secretan CC. The role of cultured chondrocytes and mesenchymal stem cells in the repair of acute articular cartilage injuries. [Doctoral Dissertation]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/r494vk77z

5. 笹井, 宣昌. Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes : 伸張刺激により引き起こされる筋管細胞肥大への PI3K/Akt/TOR 経路の関与.

Degree: 2018, Nagoya University / 名古屋大学

URL: http://hdl.handle.net/2237/14436

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Skeletal muscle cells are hypertrophied by mechanical stresses, but the underlying molecular mechanisms are not fully understood. Two signaling pathways, phosphatidylinositol 3-kinase (PI3K)/Akt to target… (more)

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Skeletal muscle cells are hypertrophied by mechanical stresses, but the underlying molecular mechanisms are not fully understood. Two signaling pathways, phosphatidylinositol 3-kinase (PI3K)/Akt to target of rapamycin (TOR) and extracellular signal-regulated kinase kinase (MEK) to extracellular signal-regulated kinase (ERK), have been proposed to be involved in muscle hypertrophy. In this study we examined the involvement of these pathways in primary cultures of chick skeletal myotubes subjected to passive cyclic stretching for 72 hours, a time that was sufficient to induce significant hypertrophy in our preparations. Hypertrophy was largely suppressed by wortmannin or rapamycin, inhibitors of PI3K or mTOR, respectively. Furthermore, phosphorylation of Akt was enhanced by stretching and suppressed by wortmannin. The MEK inhibitor, U0126, exerted a minimal influence on stretch-induced hypertrophy. We found that cyclic stretching of myotubes activates the PI3K/Akt/TOR pathway, resulting in muscle hypertrophy. The MEK/ERK pathway may contribute negatively to spontaneous hypert

骨格筋細胞は、機械ストレスにより肥大する。しかしながら、その分子メカニズムは十分に解明されてない。筋肥大に関与すると考えられている細胞シグナル経路として、 phosphatidylinositol 3-kinase (PI3K)/Akt/target of rapamycin (TOR) 経路や extracellularsignal-regulated kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) 経路がある。本研究では、それら経路が、筋細胞の伸張刺激による肥大に関連するかどうかという点について調べた。トリ胸筋の初代培養による成熟した筋管細胞に、その長軸方向の周期的伸張（伸張率 10%, 1/6 Hz, 72 時間）を加えたところ、伸張しない細胞に比べ太さが約 1.6 倍に肥大した (p < 0.05)。この伸張刺激による肥大は、 PI3K の阻害剤(wortrmannin) や、 TOR の阻害剤 (rapamycin) により抑制された。さらに伸張刺激を 5及び 60 分加えた細胞のリン酸化 Akt の割合は、刺激前に比べ凡そ 2.5 倍高かった(p < 0.05)。このシグナル亢進は wortrmannin により抑制された。一方、 MEK の阻害剤 (U0126) により、伸張刺激に関わらず細胞は肥大した。伸張刺激による肥大は殆ど変わらなかった。これらの結果から、伸張刺激による筋管細胞肥大に対しPI3K/Akt/TOR 経路が関連することや、 ERK の関連は僅かであることが示唆された。さらに ERK が、成熟した筋管の太さ制御に負に関わることも示唆された。

名古屋大学博士学位論文学位の種類:博士(リハビリテーション療法学) (課程) 学位授与年月日　平成22年3月25日

Subjects/Keywords: Akt; cultured cells; cyclic stretching; ERK; muscle hypertrophy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

笹井, �. (2018). Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes : 伸張刺激により引き起こされる筋管細胞肥大への PI3K/Akt/TOR 経路の関与. (Thesis). Nagoya University / 名古屋大学. Retrieved from http://hdl.handle.net/2237/14436

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

笹井, 宣昌. “Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes : 伸張刺激により引き起こされる筋管細胞肥大への PI3K/Akt/TOR 経路の関与.” 2018. Thesis, Nagoya University / 名古屋大学. Accessed January 19, 2021. http://hdl.handle.net/2237/14436.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

笹井, 宣昌. “Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes : 伸張刺激により引き起こされる筋管細胞肥大への PI3K/Akt/TOR 経路の関与.” 2018. Web. 19 Jan 2021.

Vancouver:

笹井 �. Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes : 伸張刺激により引き起こされる筋管細胞肥大への PI3K/Akt/TOR 経路の関与. [Internet] [Thesis]. Nagoya University / 名古屋大学; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2237/14436.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

笹井 �. Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy of myotubes : 伸張刺激により引き起こされる筋管細胞肥大への PI3K/Akt/TOR 経路の関与. [Thesis]. Nagoya University / 名古屋大学; 2018. Available from: http://hdl.handle.net/2237/14436

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of British Columbia

6. Hammerberg, Ole. Effects of combined treatments with an oncogenic chemical and virus on cultured cells.

Degree: MS- MSc, Zoology, 1972, University of British Columbia

URL: http://hdl.handle.net/2429/34091

► Metaphase chromosome aberrations were studied in cultured embryonic Syrian hamster cells after exposure to one of two potent mutagenic chemicals in combination with Adenovirus 12.… (more)

Subjects/Keywords: Carcinogens; Cell culture; Cells, Cultured

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hammerberg, O. (1972). Effects of combined treatments with an oncogenic chemical and virus on cultured cells. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/34091

Chicago Manual of Style (16^th Edition):

Hammerberg, Ole. “Effects of combined treatments with an oncogenic chemical and virus on cultured cells.” 1972. Masters Thesis, University of British Columbia. Accessed January 19, 2021. http://hdl.handle.net/2429/34091.

MLA Handbook (7^th Edition):

Hammerberg, Ole. “Effects of combined treatments with an oncogenic chemical and virus on cultured cells.” 1972. Web. 19 Jan 2021.

Vancouver:

Hammerberg O. Effects of combined treatments with an oncogenic chemical and virus on cultured cells. [Internet] [Masters thesis]. University of British Columbia; 1972. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2429/34091.

Council of Science Editors:

Hammerberg O. Effects of combined treatments with an oncogenic chemical and virus on cultured cells. [Masters Thesis]. University of British Columbia; 1972. Available from: http://hdl.handle.net/2429/34091

Oklahoma State University

7. Mahapatra, Saugata. Analysis of Coxiella Burnetii Mediated Modulation of Host Cells During Infection.

Degree: Department of Biochemistry and Molecular Biology, 2011, Oklahoma State University

URL: http://hdl.handle.net/11244/6655

► Coxiella burnetii is an obligate intracellular bacteria and the etiologic agent of Q fever. Although discovered over six decades ago, our understanding of the molecular… (more)

▼ Coxiella burnetii is an obligate intracellular bacteria and the etiologic agent of Q fever. Although discovered over six decades ago, our understanding of the molecular mechanisms involved in disease development remains elementary. Few host cell processes actively modulated by C. burnetii have been identified. This study analyses host-cell pathways and processes that are specifically affected by C. burnetii proteins. It also defines C. burnetii induced temporal modulation of NF-kB activation throughout the infectious cycle. Additionally, it determines C. burnetii's growth cycle in an established tick cell line. First, the global expression of host cell mRNA was characterized following infection with C. burnetii Nine Mile Phase II and transient inhibition of bacterial protein synthesis with chloramphenicol. Using comparative microarray analysis, 36 host cell genes were identified to be distinctively modulated by C. burnetii proteins. Subsequent gene ontology analysis revealed expression changes in host cell functions such as innate immune response, cell death and proliferation, vesicle trafficking and development, lipid homeostasis, and cytoskeletal organization. A subset of pro-inflammatory cytokine genes was also identified whose expression is classically mediated through the NF-kB signaling pathway. This led to the demonstration that C. burnetii infection temporally modulates the activation of the NF-kB signaling pathway. Additionally, I have shown that C. burnetii readily infects Ixodes scapularis-derived cultured IDE8 cells, followed by a prolonged lag phase, then a doubling time similar to that in eukaryotic cells. Together these studies show that C. burnetii replicates and produces infectious progeny in arthropod cells, and temporally modulates mammalian host cell NF-kB signaling pathway as well as host cell gene expression in a bacterial protein synthesis specific manner. Advisors/Committee Members: Shaw, Edward I. (advisor), Burnham, David K. (committee member), Picking, William D. (committee member), Hadwiger, Jeffrey A. (committee member), Little, Susan E. (committee member), Canaan, Patricia (committee member).

Subjects/Keywords: chloramphenicol; coxiella burnetii; ide8 cultured tick cells; microarray; nf-kb activation

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APA (6^th Edition):

Mahapatra, S. (2011). Analysis of Coxiella Burnetii Mediated Modulation of Host Cells During Infection. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/6655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mahapatra, Saugata. “Analysis of Coxiella Burnetii Mediated Modulation of Host Cells During Infection.” 2011. Thesis, Oklahoma State University. Accessed January 19, 2021. http://hdl.handle.net/11244/6655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mahapatra, Saugata. “Analysis of Coxiella Burnetii Mediated Modulation of Host Cells During Infection.” 2011. Web. 19 Jan 2021.

Vancouver:

Mahapatra S. Analysis of Coxiella Burnetii Mediated Modulation of Host Cells During Infection. [Internet] [Thesis]. Oklahoma State University; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11244/6655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mahapatra S. Analysis of Coxiella Burnetii Mediated Modulation of Host Cells During Infection. [Thesis]. Oklahoma State University; 2011. Available from: http://hdl.handle.net/11244/6655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Romagnoli, Graziela Gorete. Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral.

Degree: PhD, Imunologia, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18092012-104937/ ;

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Exossomos (Exo) originados de células dendríticas (DCs) carregam moléculas associadas à apresentação antigênica. Neste trabalho procurou-se estabelecer se Exo de DCs seriam capazes de conferir… (more)

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Exossomos (Exo) originados de células dendríticas (DCs) carregam moléculas associadas à apresentação antigênica. Neste trabalho procurou-se estabelecer se Exo de DCs seriam capazes de conferir imunogenicidade às células tumorais. Os Exo isolados de culturas de DCs expressavam as moléculas HLA-ABC, HLA-DR, CD86, CD11c, CD81, CD54 e CD18. Estes foram então adicionados às células da linhagem humana de adenocarcinoma mamário, SK-BR-3, as quais passaram a expressar as moléculas HLA-DR, CD86 e CD11c. As células tumorais modificadas pelos Exo induziram a produção de IL-6 e IL-10, detectados no sobrenadante das co-culturas destas com linfócitos T. Estas células tumorais também induziram aumento do número de linfócitos produtores de IFN-g, pré-sensibilizados contra antígenos tumorais, e aumento da expressão de SOCS3 nestes. Em conclusão, nossos resultados mostram que, Exo de DCs alteram o fenótipo de células tumorais, modificando sua interação com linfócitos T, sem induzir nas mesmas capacidade de ativar respostas proliferativas ou citotóxicas de linfócitos T in vitro.

Exosomes (Exo) originated from dendritic cells (DCs) contain molecules involved in antigen presentation. The present work sought to determine if Exo from DCs would be able to transfer immunogenicity to tumor cells. Exo isolated from DCs cultures carried HLA-ABC, HLA-DR, CD86, CD11c, CD81, CD54 and CD18. These Exo were added to cultures of the human breast adenocarcinoma cell line, SK-BR-3, which gained expression of HLA-DR, CD86 and CD11c. Tumor cells modified by Exo induced IL-6 and IL-10 production, detected in the supernatant of their co-cultures with T lymphocytes. These tumor cells also induced an increase in the frequency of IFN-g-producing T lymphocytes, pre-sensitized against tumor antigens, and an increased expression of SOCS3. In conclusion, our results show that, Exo from DCs affect the phenotype of tumor cells, modifying their interaction with T lymphocytes, without inducing the ability to activate cytotoxic or T cell proliferative responses in vitro.

Advisors/Committee Members: Barbuto, Jose Alexandre Marzagao.

Subjects/Keywords: Cells cultured tumor; Células cultivadas de tumor; Células dendríticas; Células tumorais; Dendritic cells; Exosomes; Exossomos; Immunotherapy; Imunoterapia; Tumor cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Romagnoli, G. G. (2012). Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18092012-104937/ ;

Chicago Manual of Style (16^th Edition):

Romagnoli, Graziela Gorete. “Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral.” 2012. Doctoral Dissertation, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18092012-104937/ ;.

MLA Handbook (7^th Edition):

Romagnoli, Graziela Gorete. “Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral.” 2012. Web. 19 Jan 2021.

Vancouver:

Romagnoli GG. Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18092012-104937/ ;.

Council of Science Editors:

Romagnoli GG. Exossomos derivados de células dendríticas como adjuvantes naturais na resposta antitumoral. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18092012-104937/ ;

University of Florida

9. Park, Yunjong. Identification of Regulatory Factors Involved in Salivary Gland Epithelial Cell Differentiation.

Degree: PhD, Medical Sciences - Immunology and Microbiology (IDP), 2014, University of Florida

URL: https://ufdc.ufl.edu/UFE0047386

► Patients with Sjogren's syndrome or head and neck cancer patients who have undergone radiation therapy suffer from severe dry mouth (xerostomia) due to salivary exocrine… (more)

Subjects/Keywords: Cells; Cultured cells; Epithelial cells; Gene therapy; Liver; Mesenchymal stem cells; Pancreas; Progenitor cells; Salivary glands; Stem cells; bm-msc – differentiation – factors – gland – salivary – sjogren – stem – transcription

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Park, Y. (2014). Identification of Regulatory Factors Involved in Salivary Gland Epithelial Cell Differentiation. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0047386

Chicago Manual of Style (16^th Edition):

Park, Yunjong. “Identification of Regulatory Factors Involved in Salivary Gland Epithelial Cell Differentiation.” 2014. Doctoral Dissertation, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0047386.

MLA Handbook (7^th Edition):

Park, Yunjong. “Identification of Regulatory Factors Involved in Salivary Gland Epithelial Cell Differentiation.” 2014. Web. 19 Jan 2021.

Vancouver:

Park Y. Identification of Regulatory Factors Involved in Salivary Gland Epithelial Cell Differentiation. [Internet] [Doctoral dissertation]. University of Florida; 2014. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0047386.

Council of Science Editors:

Park Y. Identification of Regulatory Factors Involved in Salivary Gland Epithelial Cell Differentiation. [Doctoral Dissertation]. University of Florida; 2014. Available from: https://ufdc.ufl.edu/UFE0047386

10. Hirata, Cristiane Lumi. Caracterização da sumoilação de maspina.

Degree: Mestrado, Biologia Celular e Tecidual, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17062013-082745/ ;

►

Maspina, proteína da família das serpinas, tem um único gene descrito, porém diversas funções biológicas foram observadas: modulação da adesão; inibição do crescimento, da angiogênese… (more)

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Maspina, proteína da família das serpinas, tem um único gene descrito, porém diversas funções biológicas foram observadas: modulação da adesão; inibição do crescimento, da angiogênese e da invasão tumoral; efeito pró-apoptótico entre outras. Está no núcleo, no citoplasma e na membrana plasmática. Tantas funções e localizações não podem ser justificadas apenas por sua estrutura primária. Maspina pode sofrer modificações pós-traducionais controlando sua atividade, localização subcelular e interações proteicas. Propôs-se assim, caracterizar a modificação de maspina pela adição de SUMO. Dois prováveis sítios de sumoilação, nas lisinas 47 e 277 e um provável motivo de interação com SUMO entre aminoácidos 156 e 159 foram encontrados pelos programas de predição SUMOplot, SUMOsp e GPS-SBM. A estrutura 3D de maspina dessas regiões mostra que são compatíveis e coerentes com sumoilação. Ensaio de imunoprecipitação sugere que maspina endógena é sumoilada na linhagem MCF-10A. Esses dados sugerem que sumoilação pode ser importante na regulação das funções biológicas de maspina.

Maspin, a protein from the serpin family, has only one gene described, but diverse biological functions observed: adhesion modulation; inhibition of growth, of angiogenesis and of tumoral invasion; pro-apoptotic effect and others. It is in the nucleus, the cytoplasm and the plasma membrane. Those many functions and localizations cant be justified only by its primary structure. Maspin could suffer posttranslational modifications controlling its activity, subcellular localization and protein interaction. So, we propose to characterize maspin modification by the addition of SUMO. Two possible sumoylation sites in lisines 47 and 277 and a possible SUMO interacting motif between amino acids 156 and 159 were found by prediction programs SUMOplot, SUMOsp and GPS-SBM. The 3D structure of maspin in those regions shows that they are compatible and coehrent with sumoylation. Immunoprecipitation assay suggests that endogenous maspin is sumoylated in MCF-10A cell line. This data suggest that sumoylation could be important in the regulation of maspin biological functions.

Advisors/Committee Members: Cella, Nathalie.

Subjects/Keywords: Breast; Células cultivadas; Cultured cells; Mama; Maspin; Maspina; Proteínas proto-oncogênicas; Proto-oncogene proteins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hirata, C. L. (2013). Caracterização da sumoilação de maspina. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17062013-082745/ ;

Chicago Manual of Style (16^th Edition):

Hirata, Cristiane Lumi. “Caracterização da sumoilação de maspina.” 2013. Masters Thesis, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17062013-082745/ ;.

MLA Handbook (7^th Edition):

Hirata, Cristiane Lumi. “Caracterização da sumoilação de maspina.” 2013. Web. 19 Jan 2021.

Vancouver:

Hirata CL. Caracterização da sumoilação de maspina. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17062013-082745/ ;.

Council of Science Editors:

Hirata CL. Caracterização da sumoilação de maspina. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-17062013-082745/ ;

11. Gutierrez, Vanessa Pacciari. Efeito antinociceptivo da crotalfina sobre a dor óssea induzida pelo tumor de Walker 256 em fêmur de ratos.

Degree: PhD, Farmacologia, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26092013-104400/ ;

►

Neste estudo padronizamos modelo de dor óssea induzida pela administração de células do tumor de Walker 256 em fêmur de ratos e avaliamos o efeito… (more)

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Neste estudo padronizamos modelo de dor óssea induzida pela administração de células do tumor de Walker 256 em fêmur de ratos e avaliamos o efeito analgésico da crotalfina, um peptídeo com atividade analgésica. Análises radiográficas, tomográficas e cintilográficas demonstraram a presença de processos osteolíticos difusos, destruição do tecido cortical ósseo e intensa atividade osteogênica. Foi detectado aumento de partes moles adjacentes ao osso, fenômeno observado em pacientes com câncer ósseo. Análises histopatológicas mostraram a presença de células tumorais no pulmão, baço e fígado dos animais, indicando disseminação das células tumorais. Foi detectada a presença de hiperalgesia, alodinia e dor manifesta, a partir do 1º dia após a inoculação das células, persistindo por pelo menos 14 dias. Crotalfina (8 mg/kg, p.o) administrada no 14º dia, bloqueou estes fenômenos. Esse efeito antinociceptivo é de longa duração (48 h) e mediado por receptores opióides do tipo k e d. Não foi observado desenvolvimento de tolerância após o tratamento prolongado com crotalfina.

The aim of the present work was to standardize a new rat model of bone pain induced by the injection of Walker 256 carcinoma cells into the femoral cavity of rats, and to evaluate the analgesic effect of crotalphine, an analgesic peptide. Radiographic, tomographic and scintigraphic analysis showed the presence of diffuse osteolytic processes, destruction of cortical bone tissue and intense osteogenic activity. Increase of soft tissue adjacent to the bone was also detected, being this phenomenon observed in patients with bone cancer. Hystopathological analysis showed the presence of tumor cells in lungs, spleen and liver, indicating the occurrence of metastasis. Tumor cell inoculation induced the presence of hyperalgesia, allodynia and spontaneous pain. Crotalphine (8 mg/kg, p.o.), administered on day 14, blocked these phenomena. This antinociceptive effect is long-lasting effect (2 days) and mediated by peripheral k and d- opioid receptors. Prolonged treatment with crotalphine (14 days) did not cause the development of tolerance to its antinociceptive effect.

Advisors/Committee Members: Cury, Yara.

Subjects/Keywords: Analgésicos; Analgesics; Células cultivadas de tumor; Cultured tumor cells; Dor; Femur; Fêmur; Neoplasias; Neoplasms; Pain

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APA (6^th Edition):

Gutierrez, V. P. (2013). Efeito antinociceptivo da crotalfina sobre a dor óssea induzida pelo tumor de Walker 256 em fêmur de ratos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26092013-104400/ ;

Chicago Manual of Style (16^th Edition):

Gutierrez, Vanessa Pacciari. “Efeito antinociceptivo da crotalfina sobre a dor óssea induzida pelo tumor de Walker 256 em fêmur de ratos.” 2013. Doctoral Dissertation, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26092013-104400/ ;.

MLA Handbook (7^th Edition):

Gutierrez, Vanessa Pacciari. “Efeito antinociceptivo da crotalfina sobre a dor óssea induzida pelo tumor de Walker 256 em fêmur de ratos.” 2013. Web. 19 Jan 2021.

Vancouver:

Gutierrez VP. Efeito antinociceptivo da crotalfina sobre a dor óssea induzida pelo tumor de Walker 256 em fêmur de ratos. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26092013-104400/ ;.

Council of Science Editors:

Gutierrez VP. Efeito antinociceptivo da crotalfina sobre a dor óssea induzida pelo tumor de Walker 256 em fêmur de ratos. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26092013-104400/ ;

McMaster University

12. Hiebert, Murray Bernard. Multiple Forms of Dihydrofolate Reductase in Cultured Mammalian Cells.

Degree: MSc, 1971, McMaster University

URL: http://hdl.handle.net/11375/17944

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Dihydrofolate reductase from a subline of the L1210 lymphoma was purified by affinity chromatography using substituted Sepharose -4B to which was coupled methotrexate, a… (more)

Subjects/Keywords: dihydrofolate; reductase; cultured; mammalian; cells; enzyme

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APA (6^th Edition):

Hiebert, M. B. (1971). Multiple Forms of Dihydrofolate Reductase in Cultured Mammalian Cells. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/17944

Chicago Manual of Style (16^th Edition):

Hiebert, Murray Bernard. “Multiple Forms of Dihydrofolate Reductase in Cultured Mammalian Cells.” 1971. Masters Thesis, McMaster University. Accessed January 19, 2021. http://hdl.handle.net/11375/17944.

MLA Handbook (7^th Edition):

Hiebert, Murray Bernard. “Multiple Forms of Dihydrofolate Reductase in Cultured Mammalian Cells.” 1971. Web. 19 Jan 2021.

Vancouver:

Hiebert MB. Multiple Forms of Dihydrofolate Reductase in Cultured Mammalian Cells. [Internet] [Masters thesis]. McMaster University; 1971. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11375/17944.

Council of Science Editors:

Hiebert MB. Multiple Forms of Dihydrofolate Reductase in Cultured Mammalian Cells. [Masters Thesis]. McMaster University; 1971. Available from: http://hdl.handle.net/11375/17944

Delft University of Technology

13. Steinebach, O.M. Metabolic Fate of Metallothionein in Copper and Zinc Interaction in Cultured Liver Cells.

Degree: 1993, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40

Subjects/Keywords: metallothionein; cultured liver cells; copper; zinc

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Steinebach, O. M. (1993). Metabolic Fate of Metallothionein in Copper and Zinc Interaction in Cultured Liver Cells. (Doctoral Dissertation). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40

Chicago Manual of Style (16^th Edition):

Steinebach, O M. “Metabolic Fate of Metallothionein in Copper and Zinc Interaction in Cultured Liver Cells.” 1993. Doctoral Dissertation, Delft University of Technology. Accessed January 19, 2021. http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40.

MLA Handbook (7^th Edition):

Steinebach, O M. “Metabolic Fate of Metallothionein in Copper and Zinc Interaction in Cultured Liver Cells.” 1993. Web. 19 Jan 2021.

Vancouver:

Steinebach OM. Metabolic Fate of Metallothionein in Copper and Zinc Interaction in Cultured Liver Cells. [Internet] [Doctoral dissertation]. Delft University of Technology; 1993. [cited 2021 Jan 19]. Available from: http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40.

Council of Science Editors:

Steinebach OM. Metabolic Fate of Metallothionein in Copper and Zinc Interaction in Cultured Liver Cells. [Doctoral Dissertation]. Delft University of Technology; 1993. Available from: http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; urn:NBN:nl:ui:24-uuid:974a4882-0e93-4a6b-9a75-da23dc00db40 ; http://resolver.tudelft.nl/uuid:974a4882-0e93-4a6b-9a75-da23dc00db40

14. Gorodeski, George Israel. Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells.

Degree: PhD, Physiology and Biophysics, 1990, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951

► The present study was predicated on the hypothesis that retinoids (analogues of vitamin A) have a direct effect on human female reproductive tract epithelia and… (more)

▼ The present study was predicated on the hypothesis that retinoids (analogues of vitamin A) have a direct effect on human female reproductive tract epithelia and that they modulate the effects of estradiol (E₂) and progesterone (P) on these tissues. To study retinoid and steroid regulation of differentiation in vitro I used cultured ectocervical epithelial cells (ECE) grown on 3T3 monolayers. Confluent ECE cells free of non-epithelial cells as judged by lack of expression of vimentin, stratified spontaneously, terminally differentiated and formed cornified envelopes. The epithelial origin of these cells was determined by their morphology and the expression of protein and mRNA involucrin and cytokeratins. Differentiation of the ECE cells could be modulated in vitro by diethylstilbestrol (DES) and P and the cells expressed specific E₂ binding sites (receptor). Four experiments were done: (1) Retinoids (Ro 13-6298, trans Retinoic Acid, Retinol) modulate differentiation of ECE cells in concentrations of 10^-11M to 10^-7M resulting in loss of stratification, suppression of envelopes (mainly Ro 13-6298) and increase in cell number (mainly Retinoic Acid). (2) Steroids, in concentrations of 10^-11M to 10^-6M also modulate differentiation: DES and Hydrocortisone (HC) increase while P and Testosterone suppress envelopes formation and HC increases cell number. Experiments using combinations of retinoids and steroids revealed that retinoids are more potent suppressors of envelopes than P, that DES and retinoids synergistically increase cell number and that DES renders the cells sensitive to P suppression of envelopes. (3) Changes in involucrin protein and mRNA are not the basis for envelopes regulation by retinoids and steroids. (4) Retinoids modulate cytokeratins expression: K13 (and mRNA) and K16 levels decrease and K19 (and mRNA) levels increase. Based on these results I conclude that in-vivo: (1) Vitamin A determines the basal degree of differentiation of ectocervical cells. (2) The sex steroid hormones regulate the state of differentiation of the ectocervical tissues around this set point. (3) Vitamin A regulates squamous metaplasia of the squamo-columnar junction by increasing the fraction of reserve cells. (4) Vitamin A maintains the upper reproductive tract epithelium in the form of simple columnar epithelium. Advisors/Committee Members: Eckert, Richard (Advisor).

Subjects/Keywords: Retinoids; steroid hormones; cultured; ectocervical cells

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APA (6^th Edition):

Gorodeski, G. I. (1990). Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951

Chicago Manual of Style (16^th Edition):

Gorodeski, George Israel. “Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells.” 1990. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951.

MLA Handbook (7^th Edition):

Gorodeski, George Israel. “Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells.” 1990. Web. 19 Jan 2021.

Vancouver:

Gorodeski GI. Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 1990. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951.

Council of Science Editors:

Gorodeski GI. Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 1990. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951

15. ⽚⼭, 暢仁. The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells : ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響.

Degree: 博士（歯学）, 2016, Osaka Dental University / 大阪歯科大学

URL: http://id.nii.ac.jp/1392/00000006/

►

Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the… (more)

Subjects/Keywords: Alkaline Phosphatase; Cell Differentiation; Cell Proliferation; Cells, Cultured; Collagen Type I; Dental Enamel; Flavonoids; Humans; Mesenchymal Stromal Cells; Oligopeptides; Osteocalcin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

⽚⼭, �. (2016). The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells : ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響. (Thesis). Osaka Dental University / 大阪歯科大学. Retrieved from http://id.nii.ac.jp/1392/00000006/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

⽚⼭, 暢仁. “The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells : ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響.” 2016. Thesis, Osaka Dental University / 大阪歯科大学. Accessed January 19, 2021. http://id.nii.ac.jp/1392/00000006/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

⽚⼭, 暢仁. “The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells : ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響.” 2016. Web. 19 Jan 2021.

Vancouver:

⽚⼭ �. The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells : ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響. [Internet] [Thesis]. Osaka Dental University / 大阪歯科大学; 2016. [cited 2021 Jan 19]. Available from: http://id.nii.ac.jp/1392/00000006/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

⽚⼭ �. The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells : ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響. [Thesis]. Osaka Dental University / 大阪歯科大学; 2016. Available from: http://id.nii.ac.jp/1392/00000006/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Carneiro, Juliana Laino do Val. Avaliação do papel dos miRNAs -221, -222 e -4728-3p em células-tronco tumorais derivadas de linhagens celulares de cancer de mama HER2+.

Degree: PhD, Biotecnologia, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06112013-104247/ ;

►

CSCs, caracterizadas pela alta atividade da ALDH1 e expressão (ou não) de marcadores de células-tronco embrionárias OCT-4, NANOG, SOX2, KLF4, LIN2, estão presentes em tumores… (more)

▼

CSCs, caracterizadas pela alta atividade da ALDH1 e expressão (ou não) de marcadores de células-tronco embrionárias OCT-4, NANOG, SOX2, KLF4, LIN2, estão presentes em tumores de mama HER2+. Mamosferas são estruturas celulares esferóides in vitro enriquecidas em CSCs. Neste trabalho, buscou-se um melhor entendimento do papel dos miRNAs -221, -222 e -4728-3p na biologia das CSCs. Foi observado alto percentual de células ALDH1+ (citometria) em mamosferas das linhagens MCF-7, SKBR3 e BT-474; alta expressão (qRT-PCR) de miR-221 e -222 em mamosferas da linhagem MCF-7 e de tumores de pacientes, além de alta expressão de HER2 em mamosferas das linhagens MCF-7 e SKBR3. A MFE de células MCF-7 mostrou-se aumentada após a indução (com lentivetores) da superexpressão dos miRNAs -221 e -222. A resistência ao quimioterápico paclitaxel estava aumentada em mamosferas que superexpressavam o miRNA-222. A superexpressão de miR-4728-3p, localizado em um intron de HER2, levou a um aumento das subpopulações ALDH1+ em duas linhagens celulares reforçando seu envolvimento com a biologia de CSCs.

CSCs, characterized by high activity of ALDH1 and high (or not) expression of embryonic stem cell markers OCT-4, NANOG, SOX2, KLF4, LIN28 are present in tumors with HER2 amplification. Mammospheres are spheroid cell structures in vitro enriched by CSCs. In this study, we aim to contribute to the better understanding the role of miRNAs -221, 222, 4728-3p in the the biology of CSCs. We observed high percentage of ALDH+ cells (cytometry) in mammospheres of MCF-7, SKBR3 and BT-474 cell lines; high expression (qRT-PCR) of miR-221 and -222 in mammospheres of MCF-7 cell line and cells derived from patients, moreover HER2 is upregulated in mammospheres from MCF-7 and SKBR3 cell lines. MFE is higher in MCF-7 cell line after induction (with lentivectors) of miR-221 and -222 expression. The resistance to paclitaxel was increased in mammopheres that were overexpressing miRNA-222. Overexpression of miR-4728-3p, located in an intron of HER2 gene, induced the increment of ALDH1+ subpopulations in two cell lines, reinforcing its role in the biology of CSCs.

Advisors/Committee Members: Dias Neto, Emmanuel, Nunes, Diana Noronha.

Subjects/Keywords: Breast neoplasms; Camundongos; Células cultivadas de tumor; Células-tronco; Cultured tumor cells; Mice; Neoplasias mamárias; Stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carneiro, J. L. d. V. (2013). Avaliação do papel dos miRNAs -221, -222 e -4728-3p em células-tronco tumorais derivadas de linhagens celulares de cancer de mama HER2+. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06112013-104247/ ;

Chicago Manual of Style (16^th Edition):

Carneiro, Juliana Laino do Val. “Avaliação do papel dos miRNAs -221, -222 e -4728-3p em células-tronco tumorais derivadas de linhagens celulares de cancer de mama HER2+.” 2013. Doctoral Dissertation, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06112013-104247/ ;.

MLA Handbook (7^th Edition):

Carneiro, Juliana Laino do Val. “Avaliação do papel dos miRNAs -221, -222 e -4728-3p em células-tronco tumorais derivadas de linhagens celulares de cancer de mama HER2+.” 2013. Web. 19 Jan 2021.

Vancouver:

Carneiro JLdV. Avaliação do papel dos miRNAs -221, -222 e -4728-3p em células-tronco tumorais derivadas de linhagens celulares de cancer de mama HER2+. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06112013-104247/ ;.

Council of Science Editors:

Carneiro JLdV. Avaliação do papel dos miRNAs -221, -222 e -4728-3p em células-tronco tumorais derivadas de linhagens celulares de cancer de mama HER2+. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06112013-104247/ ;

University of Florida

17. Sullivan, David C. Investigation of Organ Regeneration Through the Use of Perfusion Decellularized Scaffold Matrices.

Degree: PhD, Biomedical Engineering, 2015, University of Florida

URL: https://ufdc.ufl.edu/UFE0047739

► Organ transplantation is the current gold standard for severe organ failure of both kidney and liver patients. Annually, a donor shortage leaves thousands of Americans… (more)

▼ Organ transplantation is the current gold standard for severe organ failure of both kidney and liver patients. Annually, a donor shortage leaves thousands of Americans waiting for a life-saving transplant. One solution involves decellularizing a xenograft donor organ, rendering it homologous to a decellularized human donor graft, and infusing patient-specific cells to restore function for organ transplantation. Organ specific decellularization methods were optimized for porcine kidneys and livers as well as rodent livers as to remove all donor cellular material, yet maintain an intact vascular system for transplantation. Scaffolds were implanted under two different studies to display both minimal immunogenic response and stability of the intact vessels to maintain patent blood flow. Additionally, to understand the impact of the decellularization process and discover other uses of the generated matrix material, scaffolds were digested in an acid-enzyme solution and analyzed at each decellularization step. The resulting organ specific materials generated were liquid at room temperature and formed hydrogels at body temperature. The resulting hydrogel was paired with other materials to increase the mechanical properties and illustrated an alternative method for primary cell maintenance beyond standard tissue culture plastic. Furthermore, a parallel whole organ perfusion system was designed to maintain experimental conditions for regeneration studies at statistically significant numbers. Vessels of sterilized acellular organ matrices were attached to the apparatus and isolated primary cells infused throughout the vasculature to repopulate the entirety of scaffolds. Appropriate inlet pressure conditions were maintained throughout the experiments as to replicate implant conditions for optimal preconditioning. Preliminary results illustrated the functionality of the apparatus in maintaining homeostasis throughout the experiments in a clean, closed-system environment. Targeting appropriate cellular distribution throughout the acellular scaffold is an ongoing optimization process but overall cell viability and function under experimental conditions was maintained, underlining the promise of future products delivered from this tissue-engineered approach. ( en ) Advisors/Committee Members: MCFETRIDGE,PETER S (committee chair), DOBSON,JON P (committee member), BATICH,CHRISTOPHER D (committee member), TERADA,NAOHIRO (committee member).

Subjects/Keywords: Biomaterials; Bioreactors; Cells; Cultured cells; Hepatocytes; Kidneys; Liver; Perfusion; Scaffolds; Tissue engineering; acellular – bioreactor – decellularized – kidney – liver – scaffold

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sullivan, D. C. (2015). Investigation of Organ Regeneration Through the Use of Perfusion Decellularized Scaffold Matrices. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0047739

Chicago Manual of Style (16^th Edition):

Sullivan, David C. “Investigation of Organ Regeneration Through the Use of Perfusion Decellularized Scaffold Matrices.” 2015. Doctoral Dissertation, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0047739.

MLA Handbook (7^th Edition):

Sullivan, David C. “Investigation of Organ Regeneration Through the Use of Perfusion Decellularized Scaffold Matrices.” 2015. Web. 19 Jan 2021.

Vancouver:

Sullivan DC. Investigation of Organ Regeneration Through the Use of Perfusion Decellularized Scaffold Matrices. [Internet] [Doctoral dissertation]. University of Florida; 2015. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0047739.

Council of Science Editors:

Sullivan DC. Investigation of Organ Regeneration Through the Use of Perfusion Decellularized Scaffold Matrices. [Doctoral Dissertation]. University of Florida; 2015. Available from: https://ufdc.ufl.edu/UFE0047739

University of Florida

18. Frock, Adam. Utilizing Decellularized Porcine Liver Matrix as a Platform for 3-Dimensional Hepatic Cell Culture.

Degree: MS, Medical Sciences - Medicine, 2014, University of Florida

URL: https://ufdc.ufl.edu/UFE0047605

► The liver is responsible for numerous functions critical to maintaining homeostasis of the body, thus hepatic injury often results in mortality if an orthotropic transplant… (more)

Subjects/Keywords: Cells; Collagens; Cultured cells; Hepatocytes; Human organs; Liver; Perfusion; Rats; Scaffolds; Seeding; decellularization – ecm – ipsc – liver – regeneration – scaffold

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frock, A. (2014). Utilizing Decellularized Porcine Liver Matrix as a Platform for 3-Dimensional Hepatic Cell Culture. (Masters Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0047605

Chicago Manual of Style (16^th Edition):

Frock, Adam. “Utilizing Decellularized Porcine Liver Matrix as a Platform for 3-Dimensional Hepatic Cell Culture.” 2014. Masters Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0047605.

MLA Handbook (7^th Edition):

Frock, Adam. “Utilizing Decellularized Porcine Liver Matrix as a Platform for 3-Dimensional Hepatic Cell Culture.” 2014. Web. 19 Jan 2021.

Vancouver:

Frock A. Utilizing Decellularized Porcine Liver Matrix as a Platform for 3-Dimensional Hepatic Cell Culture. [Internet] [Masters thesis]. University of Florida; 2014. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0047605.

Council of Science Editors:

Frock A. Utilizing Decellularized Porcine Liver Matrix as a Platform for 3-Dimensional Hepatic Cell Culture. [Masters Thesis]. University of Florida; 2014. Available from: https://ufdc.ufl.edu/UFE0047605

University of Florida

19. JUNG, YOUNGMI ( Author, Primary ). Role of Somatostatin in Migration of Hepatic Oval Cells in Injured Liver Model of Rats.

Degree: University of Florida

URL: https://ufdc.ufl.edu/UFE0013700

Subjects/Keywords: Antibodies; Cell growth; Cells; Cultured cells; Ellipses; Hepatocytes; Liver; Rats; Receptors; Stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

JUNG, YOUNGMI ( Author, P. ). (n.d.). Role of Somatostatin in Migration of Hepatic Oval Cells in Injured Liver Model of Rats. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0013700

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

JUNG, YOUNGMI ( Author, Primary ). “Role of Somatostatin in Migration of Hepatic Oval Cells in Injured Liver Model of Rats.” Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0013700.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

JUNG, YOUNGMI ( Author, Primary ). “Role of Somatostatin in Migration of Hepatic Oval Cells in Injured Liver Model of Rats.” Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

JUNG, YOUNGMI ( Author P). Role of Somatostatin in Migration of Hepatic Oval Cells in Injured Liver Model of Rats. [Internet] [Thesis]. University of Florida; [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0013700.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

JUNG, YOUNGMI ( Author P). Role of Somatostatin in Migration of Hepatic Oval Cells in Injured Liver Model of Rats. [Thesis]. University of Florida; Available from: https://ufdc.ufl.edu/UFE0013700

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Florida

20. Silver, Daniel J. Investigations into the Biology of the Extracellular Matrix in Neurogenesis, Brain Injury, and Cancer.

Degree: PhD, Medical Sciences - Neuroscience (IDP), 2012, University of Florida

URL: https://ufdc.ufl.edu/UFE0044082

► Despite tremendous effort, glioblastoma multiforme (GBM) remains the most pervasive and lethal of all brain malignancies. One factor that contributes to this exceedingly poor clinical… (more)

▼ Despite tremendous effort, glioblastoma multiforme (GBM) remains the most pervasive and lethal of all brain malignancies. One factor that contributes to this exceedingly poor clinical prognosis is the highly invasive character of the tumor. Not a focused mass of cells, GBM is characterized by the microscopic infiltration of tumor cells throughout the otherwise healthy brain. Contrariwise, non-neural metastases to the brain, as well as select lower-grade gliomas can be relatively more treatable, specifically because they do not invade. Instead, these tumors develop as self-contained and clearly delineated lesions – in many respects, distinct and separate from the surrounding brain. With this dissertation, we first present evidence that the fundamental switch between these two distinct tumor pathologies – invasion and non-invasion – is mediated extrinsically, through the tumor extracellular matrix (ECM). Specifically, well-circumscribed, focused brain lesions are associated with a rich, heavily glycosylated, chondroitin sulfate proteoglycan (CSPG)-containing, tumor ECM, whereas no detectable CSPGs are associated with diffusely infiltrative brain tumors. Secondly, we extend our work to the intrinsic regulators of invasion and demonstrate that a subpopulation of highly invasive and chemoresistant glioblastoma cells is maintained by the transcription factor ZEB1 (zinc finger E-box binding homeobox 1). ZEB1 is preferentially expressed in invasive glioblastoma cells, and its knockdown results in a dramatic reduction of tumor invasion as well as increased sensitivity to the chemotherapeutic agent Temozolomide (Temodar®, TMZ) in vitro and in vivo. Lastly, we present findings on the cellular identities of the stem-like tumor cells themselves that compose a single GBM. We examine the concept that GBM may not be a single disease, but rather the culmination of multiple, discrete stem cell pathologies that ultimately manifest as GBM. In all, this work represents several unique attempts to better understand the complex biology of human brain cancer. ( en ) Advisors/Committee Members: Steindler, Dennis A (committee chair), Borchelt, David Ralph (committee member), Scott, Edward W (committee member).

Subjects/Keywords: Astrocytes; Brain neoplasms; Cells; Cultured cells; Glioma; In vitro fertilization; Lesions; Neuroglia; Stem cells; Tumors; astrocyte – brain – extracellular – invasion – matrix – microenvironment – microglia – proteoglycan – reactive – tumor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silver, D. J. (2012). Investigations into the Biology of the Extracellular Matrix in Neurogenesis, Brain Injury, and Cancer. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0044082

Chicago Manual of Style (16^th Edition):

Silver, Daniel J. “Investigations into the Biology of the Extracellular Matrix in Neurogenesis, Brain Injury, and Cancer.” 2012. Doctoral Dissertation, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0044082.

MLA Handbook (7^th Edition):

Silver, Daniel J. “Investigations into the Biology of the Extracellular Matrix in Neurogenesis, Brain Injury, and Cancer.” 2012. Web. 19 Jan 2021.

Vancouver:

Silver DJ. Investigations into the Biology of the Extracellular Matrix in Neurogenesis, Brain Injury, and Cancer. [Internet] [Doctoral dissertation]. University of Florida; 2012. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0044082.

Council of Science Editors:

Silver DJ. Investigations into the Biology of the Extracellular Matrix in Neurogenesis, Brain Injury, and Cancer. [Doctoral Dissertation]. University of Florida; 2012. Available from: https://ufdc.ufl.edu/UFE0044082

University of Florida

21. EKSIOGLU, ERIKA ADRIANA ( Author, Primary ). Dendritic Cells and Cytokine Immune Responses to Hematopoietic Stem Cell Growth Factors.

Degree: University of Florida

URL: https://ufdc.ufl.edu/UFE0013366

Subjects/Keywords: Antigens; Blood; Cells; Cultured cells; Cytokines; Dendritic cells; Immunology; Leukocytes; Tissue grafting; Tumors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

EKSIOGLU, ERIKA ADRIANA ( Author, P. ). (n.d.). Dendritic Cells and Cytokine Immune Responses to Hematopoietic Stem Cell Growth Factors. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0013366

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

EKSIOGLU, ERIKA ADRIANA ( Author, Primary ). “Dendritic Cells and Cytokine Immune Responses to Hematopoietic Stem Cell Growth Factors.” Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0013366.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

EKSIOGLU, ERIKA ADRIANA ( Author, Primary ). “Dendritic Cells and Cytokine Immune Responses to Hematopoietic Stem Cell Growth Factors.” Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

EKSIOGLU, ERIKA ADRIANA ( Author P). Dendritic Cells and Cytokine Immune Responses to Hematopoietic Stem Cell Growth Factors. [Internet] [Thesis]. University of Florida; [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0013366.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

EKSIOGLU, ERIKA ADRIANA ( Author P). Dendritic Cells and Cytokine Immune Responses to Hematopoietic Stem Cell Growth Factors. [Thesis]. University of Florida; Available from: https://ufdc.ufl.edu/UFE0013366

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Florida

22. Bleakley, Bruce Henry, 1956-. Morphology and physiology of Azospirilla grown on beta-hydroxybutyrate.

Degree: 1986, University of Florida

URL: https://ufdc.ufl.edu/AA00026412

Subjects/Keywords: Bacteria; Broths; Capsules; Carbon; Cells; Cultured cells; Cysts; Lawns; Nitrogen; Somatic cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bleakley, Bruce Henry, 1. (1986). Morphology and physiology of Azospirilla grown on beta-hydroxybutyrate. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00026412

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bleakley, Bruce Henry, 1956-. “Morphology and physiology of Azospirilla grown on beta-hydroxybutyrate.” 1986. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00026412.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bleakley, Bruce Henry, 1956-. “Morphology and physiology of Azospirilla grown on beta-hydroxybutyrate.” 1986. Web. 19 Jan 2021.

Vancouver:

Bleakley, Bruce Henry 1. Morphology and physiology of Azospirilla grown on beta-hydroxybutyrate. [Internet] [Thesis]. University of Florida; 1986. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00026412.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bleakley, Bruce Henry 1. Morphology and physiology of Azospirilla grown on beta-hydroxybutyrate. [Thesis]. University of Florida; 1986. Available from: https://ufdc.ufl.edu/AA00026412

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

23. MAATOUK, DANIELLE ( Author, Primary ). Regulatory Mechanisms Controlling Primordial Germ Cell Differentiation within the Murine Fetal Gonad.

Degree: University of Florida

URL: https://ufdc.ufl.edu/UFE0006650

Subjects/Keywords: Cells; Cultured cells; DNA; Embryos; Enzymes; Genes; Germ cells; Gonads; Methylation; Phosphatases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MAATOUK, DANIELLE ( Author, P. ). (n.d.). Regulatory Mechanisms Controlling Primordial Germ Cell Differentiation within the Murine Fetal Gonad. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0006650

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MAATOUK, DANIELLE ( Author, Primary ). “Regulatory Mechanisms Controlling Primordial Germ Cell Differentiation within the Murine Fetal Gonad.” Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0006650.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MAATOUK, DANIELLE ( Author, Primary ). “Regulatory Mechanisms Controlling Primordial Germ Cell Differentiation within the Murine Fetal Gonad.” Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

MAATOUK, DANIELLE ( Author P). Regulatory Mechanisms Controlling Primordial Germ Cell Differentiation within the Murine Fetal Gonad. [Internet] [Thesis]. University of Florida; [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0006650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

MAATOUK, DANIELLE ( Author P). Regulatory Mechanisms Controlling Primordial Germ Cell Differentiation within the Murine Fetal Gonad. [Thesis]. University of Florida; Available from: https://ufdc.ufl.edu/UFE0006650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

24. Soltys, Patricia Ann. The metabolism of lipoproteins in primary cultures of rabbit hepatocytes.

Degree: PhD, 1980, Oregon Health Sciences University

URL: doi:10.6083/M4J67F4P ; http://digitalcommons.ohsu.edu/etd/2310

Subjects/Keywords: Cells, Cultured; Lipoproteins – metabolism; Liver – metabolism; Rabbits

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APA (6^th Edition):

Soltys, P. A. (1980). The metabolism of lipoproteins in primary cultures of rabbit hepatocytes. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M4J67F4P ; http://digitalcommons.ohsu.edu/etd/2310

Chicago Manual of Style (16^th Edition):

Soltys, Patricia Ann. “The metabolism of lipoproteins in primary cultures of rabbit hepatocytes.” 1980. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 19, 2021. doi:10.6083/M4J67F4P ; http://digitalcommons.ohsu.edu/etd/2310.

MLA Handbook (7^th Edition):

Soltys, Patricia Ann. “The metabolism of lipoproteins in primary cultures of rabbit hepatocytes.” 1980. Web. 19 Jan 2021.

Vancouver:

Soltys PA. The metabolism of lipoproteins in primary cultures of rabbit hepatocytes. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 1980. [cited 2021 Jan 19]. Available from: doi:10.6083/M4J67F4P ; http://digitalcommons.ohsu.edu/etd/2310.

Council of Science Editors:

Soltys PA. The metabolism of lipoproteins in primary cultures of rabbit hepatocytes. [Doctoral Dissertation]. Oregon Health Sciences University; 1980. Available from: doi:10.6083/M4J67F4P ; http://digitalcommons.ohsu.edu/etd/2310

25. Root, Leslie L. Production of acidic and basic fibroblast growth factors and their receptors by normal human fibroblasts.

Degree: PhD, 1994, Oregon Health Sciences University

URL: doi:10.6083/M45B00Q7 ; http://digitalcommons.ohsu.edu/etd/2816

Subjects/Keywords: Fibroblast Growth Factors; RNA, Messenger; Skin; Cells, Cultured

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Root, L. L. (1994). Production of acidic and basic fibroblast growth factors and their receptors by normal human fibroblasts. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M45B00Q7 ; http://digitalcommons.ohsu.edu/etd/2816

Chicago Manual of Style (16^th Edition):

Root, Leslie L. “Production of acidic and basic fibroblast growth factors and their receptors by normal human fibroblasts.” 1994. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 19, 2021. doi:10.6083/M45B00Q7 ; http://digitalcommons.ohsu.edu/etd/2816.

MLA Handbook (7^th Edition):

Root, Leslie L. “Production of acidic and basic fibroblast growth factors and their receptors by normal human fibroblasts.” 1994. Web. 19 Jan 2021.

Vancouver:

Root LL. Production of acidic and basic fibroblast growth factors and their receptors by normal human fibroblasts. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 1994. [cited 2021 Jan 19]. Available from: doi:10.6083/M45B00Q7 ; http://digitalcommons.ohsu.edu/etd/2816.

Council of Science Editors:

Root LL. Production of acidic and basic fibroblast growth factors and their receptors by normal human fibroblasts. [Doctoral Dissertation]. Oregon Health Sciences University; 1994. Available from: doi:10.6083/M45B00Q7 ; http://digitalcommons.ohsu.edu/etd/2816

26. Rosenbloom, Jay S. The Role of potentially phosphorylated serines in desensitization of the endothelin-A receptor.

Degree: PhD, 1994, Oregon Health Sciences University

URL: doi:10.6083/M45H7DHW ; http://digitalcommons.ohsu.edu/etd/2785

Subjects/Keywords: Phosphoserine; Serine; Receptors, Endothelin; Tachyphylaxis; Cells, Cultured

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rosenbloom, J. S. (1994). The Role of potentially phosphorylated serines in desensitization of the endothelin-A receptor. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M45H7DHW ; http://digitalcommons.ohsu.edu/etd/2785

Chicago Manual of Style (16^th Edition):

Rosenbloom, Jay S. “The Role of potentially phosphorylated serines in desensitization of the endothelin-A receptor.” 1994. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 19, 2021. doi:10.6083/M45H7DHW ; http://digitalcommons.ohsu.edu/etd/2785.

MLA Handbook (7^th Edition):

Rosenbloom, Jay S. “The Role of potentially phosphorylated serines in desensitization of the endothelin-A receptor.” 1994. Web. 19 Jan 2021.

Vancouver:

Rosenbloom JS. The Role of potentially phosphorylated serines in desensitization of the endothelin-A receptor. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 1994. [cited 2021 Jan 19]. Available from: doi:10.6083/M45H7DHW ; http://digitalcommons.ohsu.edu/etd/2785.

Council of Science Editors:

Rosenbloom JS. The Role of potentially phosphorylated serines in desensitization of the endothelin-A receptor. [Doctoral Dissertation]. Oregon Health Sciences University; 1994. Available from: doi:10.6083/M45H7DHW ; http://digitalcommons.ohsu.edu/etd/2785

27. ⻑家, 茉耶. A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model : タバコ煙成分がラットの⻭の移動に及ぼす実験的研究.

Degree: 博士（歯学）, 2016, Osaka Dental University / 大阪歯科大学

URL: http://id.nii.ac.jp/1392/00000007/

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Tobacco smoke is a complex mixture of numerous components. Nevertheless, most experiments have examined the effects of individual chemicals in tobacco smoke. The comprehensive effects… (more)

Subjects/Keywords: Acid Phosphatase; Animals; Bone Density Conservation Agents; Bone Resorption; Cell Line; Cells, Cultured; Isoenzymes; Nicotine; Osteoclasts; Rats; Smoke; Tobacco; Tooth Movement

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

⻑家, �. (2016). A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model : タバコ煙成分がラットの⻭の移動に及ぼす実験的研究. (Thesis). Osaka Dental University / 大阪歯科大学. Retrieved from http://id.nii.ac.jp/1392/00000007/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

⻑家, 茉耶. “A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model : タバコ煙成分がラットの⻭の移動に及ぼす実験的研究.” 2016. Thesis, Osaka Dental University / 大阪歯科大学. Accessed January 19, 2021. http://id.nii.ac.jp/1392/00000007/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

⻑家, 茉耶. “A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model : タバコ煙成分がラットの⻭の移動に及ぼす実験的研究.” 2016. Web. 19 Jan 2021.

Vancouver:

⻑家 �. A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model : タバコ煙成分がラットの⻭の移動に及ぼす実験的研究. [Internet] [Thesis]. Osaka Dental University / 大阪歯科大学; 2016. [cited 2021 Jan 19]. Available from: http://id.nii.ac.jp/1392/00000007/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

⻑家 �. A comprehensive mixture of tobacco smoke components retards orthodontic tooth movement via the inhibition of osteoclastogenesis in a rat model : タバコ煙成分がラットの⻭の移動に及ぼす実験的研究. [Thesis]. Osaka Dental University / 大阪歯科大学; 2016. Available from: http://id.nii.ac.jp/1392/00000007/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Victo, Nathália Cruz de. Avaliação da morte celular induzida pela associação de paclitaxel e cisplatina em linhagens celulares derivadas de tumor de cabeça e pescoço.

Degree: Mestrado, Imunologia, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-24052014-111904/ ;

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Os tumores de cabeça e pescoço ocupam o sexto lugar no ranking de incidência mundial, e estão localizados na região da face, fossas nasais, seios… (more)

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Os tumores de cabeça e pescoço ocupam o sexto lugar no ranking de incidência mundial, e estão localizados na região da face, fossas nasais, seios paranasais, boca, faringe, laringe entre outros tecidos moles do pescoço. O tabagismo, o etilismo, a radiação solar e o vírus HPV, são fatores de risco associados a esse tipo de tumors. A terapia combinada de drogas antineoplásicas tem sido utilizada para amenizar os efeitos colaterais e potencializar o tratamento antitumoral. A combinação de paclitaxel e cisplatina tem se mostrado eficaz em tumores sólidos, como o HNSCC. A morte celular induzida pelo paclitaxel é mediada pela ruptura da dinâmica dos microtúbulos normais, já a cisplatina induz ligações cruzadas de DNA estes tratamentos induzem a célula à morte por apoptose. A apoptose é um processo de morte dividido, didaticamente, em via intrínseca (via mitocondrial) e via extrínseca (via receptor de morte). Entender por qual via essas células tumorais são induzidas a morte é fundamental para tentar evitar a resistência dessas células ao tratamento. Nosso objetivo é entender se células tumorais de HNSCC são resistentes ou sensíveis ao tratamento com paclitaxel e cisplatina sozinhos ou em associação. Para isso, realizamos o tratamento da linhagem celular FaDu por um período de 48 horas com as drogas e verificamos sua resistência a indução de morte por esses tratamentos. Os resultados mostraram que o tratamento com paclitaxel não potencializa a morte desta célula, sendo a linhagem FaDu mais sensível ao tratamento com cisplatina e a associação apresenta o mesmo perfil de quando tratada com cisplatina. A morte desta linhagem é dependente de caspases e possui uma preferência pela via extrínseca da apoptose que, consequentemente, induz a morte também pela via intrínseca. A modulação desta morte se dá pelo balanço das proteínas pró- e anti-apoptóticas da família BCL-2 que são responsáveis pela regulação da apoptose, o tratamento com cisplatina induz a expressão da proteína pró-apoptótica BAK e a diminuição das proteínas anti-apoptóticas BCL-2 e BCL-XL. Com os resultados obtidos, concluímos que a associação de paclitaxel e cisplatina não potencializa a morte da linhagem celular FaDu. Contudo, a cisplatina apresentou-se efetiva na indução de morte por apoptose através do aumento da expressão da proteína BAK e a diminuição da expressão das proteínas BCL-2 e BCL-XL.

Head and neck squamous cell carcinoma is the sixth most common cancer in the world, and are located in the region of the face, nasal fossas, sinuses, mouth, pharynx, larynx and other soft tissues of the neck. Tobacco smoke, etilism, solar radiation and HPV are risk factors associated with this type of tumors. Combination therapy of anticancer drugs has been used to alleviate the side effects and potentiate the antitumor treatment. The combination of paclitaxel and cisplatin has been shown to be effective in solid tumors such as HNSCC. The paclitaxel-induced cell death is mediated by disruption of the normal microtubule dynamics, and the cisplatin induced DNA…

Advisors/Committee Members: Jacysyn, Jacqueline de Fátima.

Subjects/Keywords: Antineoplásicos; Antineoplastic; Apoptose; Apoptosis; Carcinoma; Carcinoma; Células cultivadas de tumor; Chemotherapeutic; Cisplatin; Cisplatina; Cultured tumor cells; Quimioterápicos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Victo, N. C. d. (2013). Avaliação da morte celular induzida pela associação de paclitaxel e cisplatina em linhagens celulares derivadas de tumor de cabeça e pescoço. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42133/tde-24052014-111904/ ;

Chicago Manual of Style (16^th Edition):

Victo, Nathália Cruz de. “Avaliação da morte celular induzida pela associação de paclitaxel e cisplatina em linhagens celulares derivadas de tumor de cabeça e pescoço.” 2013. Masters Thesis, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-24052014-111904/ ;.

MLA Handbook (7^th Edition):

Victo, Nathália Cruz de. “Avaliação da morte celular induzida pela associação de paclitaxel e cisplatina em linhagens celulares derivadas de tumor de cabeça e pescoço.” 2013. Web. 19 Jan 2021.

Vancouver:

Victo NCd. Avaliação da morte celular induzida pela associação de paclitaxel e cisplatina em linhagens celulares derivadas de tumor de cabeça e pescoço. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-24052014-111904/ ;.

Council of Science Editors:

Victo NCd. Avaliação da morte celular induzida pela associação de paclitaxel e cisplatina em linhagens celulares derivadas de tumor de cabeça e pescoço. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-24052014-111904/ ;

29. Menezes, Michelle dos Santos. O papel do jaspamídeo na dinâmica do citoesqueleto de actina das células de melanoma: relação com migração e invasão.

Degree: Mestrado, Biologia Celular e Tecidual, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-19032012-145318/ ;

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No processo de metástase os movimentos de migração e invasão tem um papel essencial e em ambos a função dos microfilamentos é de grande importância.… (more)

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No processo de metástase os movimentos de migração e invasão tem um papel essencial e em ambos a função dos microfilamentos é de grande importância. Neste contexto, o presente trabalho buscoui analisar os efeitos da droga jaspamídeo e após a determinação das concentrações de IC50 para as linhagens HT144 e NGM foram estudados os efeitos da droga. Os tratamentos mostraram que o fármaco atua desorganizando o citoesqueleto de modo dependente de sua concentração. Os ensaios de ferida mostraram diminuição da taxa de fechamento da área livre após os tratamentos e os ensaios de migração com placas de transwell mostraram que os grupos tratados sofrem aumento desse parâmetro. Nos ensaios de invasão com câmara de Boyden o tratamento mostrou-se efetivo apenas para a célula NGM quando tratadas com 75 nM de jaspamídeo e 30 mM de Y-27632. Quanto à migração, a linhagem NGM não completava este processo quando tratada com as concentrações do IC50 e do IC50/2 acrescido de Y-27632 e a linhagem HT144 apresenta aumento deste parâmetro quando tratado com jaspamídeo e 200 mM de NSC23766.

Many signaling ways are involved in metastasis and the cellular migration and invasion are important in this context. The role of microfilaments is essential in mesenchymal and amoeboid migration. In this context, the present work aimed to analyze the effects of the drug jaspamide and. after the determination of the IC50 concentrations for the HT144 and NGM cell lines, the effects of the treatment with jaspamide were studied. The wound assays indicated a decrease in the free area after the treatments, and the migration assays with transwell showed that, after the inoculation with the drug, the cells increased the process of migration. In the invasion assays with Boydens chamber, the treatment with jaspamide was effective only in NGM cells, when they are treated with 75 nM of the drug plus 30 mM of Y-27632. Regarding the migration process, the NGM cell line did not show movement when treated with the IC50 concentration and the IC50/2 concentration plus Y-27632, and the HT144 cell line increases this parameter when treated with jaspamide and 200 mM of NSC23766.

Advisors/Committee Members: Santelli, Glaucia Maria Machado.

Subjects/Keywords: Actinas; Actins; Células cultivadas de tumor; Citoesqueleto; Cultured tumor cells; Cytoskeleton; Drugs; Fármacos; Melanoma; Melanoma; Metástase neoplásica; Neoplasm metastasis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Menezes, M. d. S. (2011). O papel do jaspamídeo na dinâmica do citoesqueleto de actina das células de melanoma: relação com migração e invasão. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42134/tde-19032012-145318/ ;

Chicago Manual of Style (16^th Edition):

Menezes, Michelle dos Santos. “O papel do jaspamídeo na dinâmica do citoesqueleto de actina das células de melanoma: relação com migração e invasão.” 2011. Masters Thesis, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-19032012-145318/ ;.

MLA Handbook (7^th Edition):

Menezes, Michelle dos Santos. “O papel do jaspamídeo na dinâmica do citoesqueleto de actina das células de melanoma: relação com migração e invasão.” 2011. Web. 19 Jan 2021.

Vancouver:

Menezes MdS. O papel do jaspamídeo na dinâmica do citoesqueleto de actina das células de melanoma: relação com migração e invasão. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-19032012-145318/ ;.

Council of Science Editors:

Menezes MdS. O papel do jaspamídeo na dinâmica do citoesqueleto de actina das células de melanoma: relação com migração e invasão. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-19032012-145318/ ;

30. Guzovsky, Eric Marcel. Análise de alterações histopatológicas e celulares em tecidos de camundongos C57BL/6J inoculados com células de melanoma experimental B16F10 por via subcultanea e tratados com o imunomodulador Imiquimod 5%.

Degree: Mestrado, Biotecnologia, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30052012-092333/ ;

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Imiquimod é uma imidazoquinolina, agonista do TLR-7, ativador de células dendríticas (DC) e indutor da transcrição de diversas citocinas. O objetivo deste estudo é avaliar… (more)

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Imiquimod é uma imidazoquinolina, agonista do TLR-7, ativador de células dendríticas (DC) e indutor da transcrição de diversas citocinas. O objetivo deste estudo é avaliar o crescimento tumoral, a formação de metástases em órgãos internos, a atividade do sistema imune e o tipo de morte celular induzido devido ao tratamento tópico. Camundongos foram inoculados com células de melanoma murino B16F10. O fármaco, na dose de 5mg, foi aplicado no local da inoculação uma vez por dia até o fim do experimento. Foram estudados quatro grupos experimentais, dois inoculado com células tumorais, sendo um tratado e outro não, e outros dois grupos, um recebeu somente o tratamento com o fármaco e o outro não recebeu tratamento. Os animais foram pesados e seus tumores medidos periodicamente. Exemplares dos grupos foram sacrificados em quatro tempos diferentes, imediatamente submetidos à necropsia para a análise de massas tumorais por citometria de fluxo com caspase-3 e anexina-v para investigar a indução de apoptose e/ ou necrose tumoral, e remoção do tumor, pele, Baço, linfonodo e pulmão, que foram fixados, inclusos em parafina, cortados e corados com HE, para análise histológica.

Imiquimod is a TLR-7 agonist and activator of dendritic cells (DC), and induces transcription of several cytokines. This study`s objective is to evaluate tumor growth, development of metastasis within internal organs and the immune system`s function, due to the topical treatment. Two groups of C57BL/6J mice were inoculated with B16F10 melanoma cells subcutaneously, and only one of the groups was treated, applied topically at the injection site once a day, followed by a second round of two groups, one treated and the other as a healthy group. Every two tumors and weight were measured. In order to observe the tumor progression and treatment effects, groups of animals were sacrificed on four days apart and immediately submitted to necropsy for a tumor analysis by flow cytometry, to evaluate the induction of apoptosis and necrosis, and to macroscopically observe the tumor development within internal organs, vascularization and posterior inclusion of the organs in paraffin for histological analysis. Tumor, skin, spleen, limph node and lung were colored with HE for histological analysis of migrating immune system cells and tumoral cells in the organs and describing its integrity.

Advisors/Committee Members: Vassao, Ruth Camargo.

Subjects/Keywords: Adjuvantes imunológico; Animal histopathology; Camundongos; Células cultivada de tumor; Cultured tumor cells; Histopatologia animal; Imidazoquinolina; Imidazoquinolina; Immune adjuvants; Immunomodulation; Imunomodulação; Mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guzovsky, E. M. (2011). Análise de alterações histopatológicas e celulares em tecidos de camundongos C57BL/6J inoculados com células de melanoma experimental B16F10 por via subcultanea e tratados com o imunomodulador Imiquimod 5%. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30052012-092333/ ;

Chicago Manual of Style (16^th Edition):

Guzovsky, Eric Marcel. “Análise de alterações histopatológicas e celulares em tecidos de camundongos C57BL/6J inoculados com células de melanoma experimental B16F10 por via subcultanea e tratados com o imunomodulador Imiquimod 5%.” 2011. Masters Thesis, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30052012-092333/ ;.

MLA Handbook (7^th Edition):

Guzovsky, Eric Marcel. “Análise de alterações histopatológicas e celulares em tecidos de camundongos C57BL/6J inoculados com células de melanoma experimental B16F10 por via subcultanea e tratados com o imunomodulador Imiquimod 5%.” 2011. Web. 19 Jan 2021.

Vancouver:

Guzovsky EM. Análise de alterações histopatológicas e celulares em tecidos de camundongos C57BL/6J inoculados com células de melanoma experimental B16F10 por via subcultanea e tratados com o imunomodulador Imiquimod 5%. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30052012-092333/ ;.

Council of Science Editors:

Guzovsky EM. Análise de alterações histopatológicas e celulares em tecidos de camundongos C57BL/6J inoculados com células de melanoma experimental B16F10 por via subcultanea e tratados com o imunomodulador Imiquimod 5%. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30052012-092333/ ;

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