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You searched for subject:(Cry1F). Showing records 1 – 3 of 3 total matches.

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Louisiana State University

1. Dangal, Vikash. Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida.

Degree: MS, Entomology, 2014, Louisiana State University

The fall armyworm (Spodoptera frugiperda (J. E. Smith)) is a major pest targeted by transgenic corn expressing Bacillus thuringiensis (Bt) proteins in both North and South America. The objective of this study was to characterize the inheritance and fitness costs of the Cry1F resistance in two fall armyworm populations collected from Puerto Rico (RR-PR) and Florida (RR-FL). To determine the inheritance of the resistance, besides RR-PR, RR-FL, and a Cry1F-susceptible population (Bt-SS), 14 other populations were developed by reciprocal crosses, F1 by F1 crosses, backcrosses, and crosses between RR-PR and RR-FL. Diet-incorporated bioassays were conducted to determine the Cry1F susceptibility for all 17 populations. To assess the fitness costs of the resistance, seven insect populations were assayed on a non-toxic diet as well as on a combined rearing of non-Bt corn leaf tissue and non-Bt diet. The seven populations were RR-PR, RR-FL, Bt-SS, and four F1 populations that were developed from the reciprocal crosses between Bt-SS and the two resistant populations. Biological parameters measured in the fitness tests were neonate-to-adult survivorship, neonate-to-adult development time, 10-day larval mass on non-Bt corn leaf tissue, pupal mass, and sex ratios. The results showed that there might be a different genetic basis for the Cry1F resistance between the Puerto Rico and Florida populations. The Cry1F resistance in RR-PR was likely inherited in >1 recessive or incompletely recessive genes and the genes associated the resistance were sex-linked to the males of the insect. In contrast, the resistance in RR-FL was dominant and more likely controlled by autosomal genes. Cry1F resistance in both resistant populations was associated with considerable fitness costs, especially for the Florida population. The fitness costs in the Cry1F-resistant fall armyworm were revealed in reduced growth, increased mortality, and delayed development. Data generated from this study should be valuable in understanding the mechanisms of Cry1F resistance in fall armyworm and developing effective strategies for resistance management.

Subjects/Keywords: Inheritance; fitness costs; Cry1F; fall armyworm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Dangal, V. (2014). Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida. (Masters Thesis). Louisiana State University. Retrieved from etd-11022014-153444 ; https://digitalcommons.lsu.edu/gradschool_theses/3144

Chicago Manual of Style (16th Edition):

Dangal, Vikash. “Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida.” 2014. Masters Thesis, Louisiana State University. Accessed January 29, 2020. etd-11022014-153444 ; https://digitalcommons.lsu.edu/gradschool_theses/3144.

MLA Handbook (7th Edition):

Dangal, Vikash. “Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida.” 2014. Web. 29 Jan 2020.

Vancouver:

Dangal V. Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2020 Jan 29]. Available from: etd-11022014-153444 ; https://digitalcommons.lsu.edu/gradschool_theses/3144.

Council of Science Editors:

Dangal V. Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-11022014-153444 ; https://digitalcommons.lsu.edu/gradschool_theses/3144

2. Souza, Dariane Sagaseta de Oliveira. Suscetibilidade de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) à proteína inseticida Cry1F de Bacillus thuringiensis Berliner no Brasil.

Degree: Mestrado, Entomologia, 2013, University of São Paulo

A broca-da-cana Diatraeasaccharalis(Fabricius) é uma das pragas-alvo do milho geneticamente modificado que expressagene(s) que codifica(m) proteína(s) de BacillusthuringiensisBerliner(milho Bt) no Brasil. Para estabelecer estratégias proativas de manejo de resistência, os objetivos do presente trabalho foram (1)estabelecer linhas-básicas de suscetibilidade à proteína Cry1F de B. thuringiensisem populações de D. saccharalis coletadas nas principais regiões produtoras de milho no Brasil na safra de 2010/2011, (2) realizar monitoramentoda suscetibilidade de populações de D. saccharalis na safra 2011/2012 e (3) avaliar a atividade biológica do milho Bt (evento TC1507) em diferentes estádios fenológicos no controle de D. saccharalis em condições laboratoriais. Para a caracterização das linhas-básicas de suscetibilidade foram realizados bioensaios com a proteína purificada Cry1F aplicada superficialmente em dieta artificial.As CL50 de Cry1F estimadas variaram de 0,47 a 7,02 ng de Cry1F/ cm2 dieta artificial para as populações avaliadas (variação de ?15 vezes). Foi definida e validada a concentração diagnóstica de 112 ng de Cry1F/cm²,baseada na estimativa da CL99 das populações testadas para o monitoramento da suscetibilidade. A avaliação da atividade biológica de milho Bt (evento TC1507) foi realizada com dois híbridos (Pioneer 30F35 e Dow 2B688) nos estádios fenológicos V3, V6 e V9 e em duas épocas de plantio. Alta atividade de controle de D. saccharalis foi obtida para os dois híbridos de milho Bt nos diferentes estádios fenológicos e épocas de plantio. Portanto, verificou-se que a espécie D. saccharalis é altamente suscetível à proteína Cry1F. Contudo, a implementação de estratégias de manejo da resistência é de fundamental importância para preservar a vida útil da tecnologia de milho Bt como importante ferramenta em programas de MIP.

The sugarcane borer Diatraeasaccharalis (Fabricius) is one of the target pests of genetically modified maizeexpressinggenes that code for insecticidal proteins from Bacillus thuringiensis Berliner (Bt maize) in Brazil. To implement proactive resistance management strategies, we leaded studies (1) to establish baseline susceptibility to Cry1F protein from B. thuringiensis in populations of D. saccharalis collected from major maize production regions in Brazil during 2010/2011 growing season, (2) to leadsusceptibility monitoring in populations of D. saccharalis collected in 2011/2012 growing season, and (3) to assess the biological activity of Bt maize (event TC1507) at different growth stages forD. saccharaliscontrol under laboratory conditions. The baseline susceptibility wascharacterizedwith purified protein Cry1F applied superficially on artificial diet. The estimatesof LC50 of Cry1F ranged from 0.47 to 7.02 ng Cry1F/cm2of artificial diet for the populations evaluated (? 15-fold variation).A diagnostic concentration of 112 ng Cry1F/cm ² was defined and validated for monitoring the susceptibility based on estimation of CL99 of all tested populations. The biological activity of Bt maize…

Advisors/Committee Members: Omoto, Celso.

Subjects/Keywords: Bacillaceae; Bactérias gram-positivas; Brocas - Insetos nocivos; Bt maize; Cry1F; Diatraeasaccharalis; Manejo integrado; Milho; Plantas transgênicas; Proteínas de plantas; Resistance management

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Souza, D. S. d. O. (2013). Suscetibilidade de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) à proteína inseticida Cry1F de Bacillus thuringiensis Berliner no Brasil. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11146/tde-25062013-165208/ ;

Chicago Manual of Style (16th Edition):

Souza, Dariane Sagaseta de Oliveira. “Suscetibilidade de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) à proteína inseticida Cry1F de Bacillus thuringiensis Berliner no Brasil.” 2013. Masters Thesis, University of São Paulo. Accessed January 29, 2020. http://www.teses.usp.br/teses/disponiveis/11/11146/tde-25062013-165208/ ;.

MLA Handbook (7th Edition):

Souza, Dariane Sagaseta de Oliveira. “Suscetibilidade de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) à proteína inseticida Cry1F de Bacillus thuringiensis Berliner no Brasil.” 2013. Web. 29 Jan 2020.

Vancouver:

Souza DSdO. Suscetibilidade de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) à proteína inseticida Cry1F de Bacillus thuringiensis Berliner no Brasil. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2020 Jan 29]. Available from: http://www.teses.usp.br/teses/disponiveis/11/11146/tde-25062013-165208/ ;.

Council of Science Editors:

Souza DSdO. Suscetibilidade de Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) à proteína inseticida Cry1F de Bacillus thuringiensis Berliner no Brasil. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/11/11146/tde-25062013-165208/ ;


University of Florida

3. Luciani, Gabriela F. Genetic Engineering to Improve Nutritional Quality and Pest Resistance in Bahiagrass (Paspalum notatum var. Flugge).

Degree: PhD, Agronomy, 2007, University of Florida

Bahiagrass (Paspalum notatum var. Flugge) is the predominant grass in forage pastures in tropical and subtropical regions worldwide. It supports the beef and dairy cattle industries in the state of Florida. To improve forage production and animal performance, the low nutritional quality and the insect pest susceptibility of this subtropical grass should be addressed. To improve nutritional quality, the vspB gene encoding a soybean VSP &946; that is a lysine-enriched and rumen proteolysis-resistant protein seems to be a promising candidate. To improve insect pest resistance, the cry1F gene encoding a & #948;-endotoxin from Bacillus thuringiensis that provides protection against Lepidopteran pests in commercial crops such as cotton and corn seems to be a suitable candidate. Our objectives were: to overexpress the vspB gene for improving nutritional in bahiagrass cv. & #8220;Argentine & #8221;, and to express a synthetic cry1F gene for enhancing resistance against fall armyworm in bahiagrass cvs. & #8220;Argentine & #8221; and & #8220;Tifton 9. & #8221; To overexpress the vspB gene, we cloned an expression vector containing a constitutive and strongly expressed promoter for monocots; a KDEL signal for ER-retention and a c-myc tag for antibody detection. We cobombarded mature seed-derived calli from bahiagrass cv. & #8220;Argentine & #8221; with minimal constructs containing the vspB gene from pGL4 and pRSVP1 vectors and the nptII gene as selectable marker from pJFNPTII vector. Following previous reports, we generated 91 nptII(+) plants from 110 analyzed plants. Based on the results from nptII expression detected by ELISA and VSP & #946; expression detected by western blots, 89% of the plants coexpressed the nptII and the vspB genes. Western blot analyses indicated that VSP & #946; expression levels varied among plants, tillers within plants and leaves within tillers. This is the first report on expression of the vspB gene in bahiagrass cv. & #8220;Argentine. & #8221; The VSP & #946; expression levels observed in transgenic bahiagrass plants were low and similar to those previously reported in transgenic corn plants. Potentially, these VSP & #946; expression levels could be enhanced by coexpressing recombinant VSP & #946; and VSP & #945; subunits, and/or by targeting the subunit to different cell compartments in transgenic bahiagrass plants. To express the synthetic cry1F gene, we co-bombarded mature seed-derived calli from bahiagrass with minimal constructs containing the synthetic cry1F gene from pHZCRY vector and the nptII gene as selectable marker from pHZ35SNPTII vector. Based on previous reports, we regenerated three paromomycin-resistant plants from bahiagrass cvs. & #8220;Argentine & #8221; and & #8220;Tifton 9. & #8221; PCR and southern blots analyses indicated independent transgene integration, and RT-PCR analyses confirmed cry1F expression in all transgenic bahiagrass lines. Two immunoassays for cry1F gene expression indicated detectable cry1F levels in two bahiagrass lines from cv. & #8220;Tifton 9. & #8221;… Advisors/Committee Members: Wofford, David S. (committee chair), Boote, Kenneth J. (committee member), Smith, Rex L. (committee member), Lyrene, Paul M. (committee member).

Subjects/Keywords: Centrifugation; Corn; Crops; Gels; Insects; Leaves; Polymerase chain reaction; Soybeans; Transgenes; Transgenic plants; bahiagrass, cry1f, notatum, nutritional, paspalum, pest, quality, resistance, vspb

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Luciani, G. F. (2007). Genetic Engineering to Improve Nutritional Quality and Pest Resistance in Bahiagrass (Paspalum notatum var. Flugge). (Doctoral Dissertation). University of Florida. Retrieved from http://ufdc.ufl.edu/UFE0019820

Chicago Manual of Style (16th Edition):

Luciani, Gabriela F. “Genetic Engineering to Improve Nutritional Quality and Pest Resistance in Bahiagrass (Paspalum notatum var. Flugge).” 2007. Doctoral Dissertation, University of Florida. Accessed January 29, 2020. http://ufdc.ufl.edu/UFE0019820.

MLA Handbook (7th Edition):

Luciani, Gabriela F. “Genetic Engineering to Improve Nutritional Quality and Pest Resistance in Bahiagrass (Paspalum notatum var. Flugge).” 2007. Web. 29 Jan 2020.

Vancouver:

Luciani GF. Genetic Engineering to Improve Nutritional Quality and Pest Resistance in Bahiagrass (Paspalum notatum var. Flugge). [Internet] [Doctoral dissertation]. University of Florida; 2007. [cited 2020 Jan 29]. Available from: http://ufdc.ufl.edu/UFE0019820.

Council of Science Editors:

Luciani GF. Genetic Engineering to Improve Nutritional Quality and Pest Resistance in Bahiagrass (Paspalum notatum var. Flugge). [Doctoral Dissertation]. University of Florida; 2007. Available from: http://ufdc.ufl.edu/UFE0019820

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