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You searched for subject:(Cox20). Showing records 1 – 2 of 2 total matches.

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Cornell University

1. Elliott, Leah. Analysis Of The Assembly Of Saccharomyces Cerevisiae Cytochrome C Oxidase Subunit Cox2 By The Oxa1 And Cox18 Translocases.

Degree: PhD, Genetics, 2013, Cornell University

Oxa1 and Cox18 are members of a conserved protein family of integral inner membrane translocases responsible for insertion of other proteins into the membranes of chloroplasts, bacteria and mitochondria. In Saccharomyces cerevisiae both Oxa1 and Cox18 are required for the translocation of the hydrophilic domains of cytochrome c oxidase subunit Cox2 into the inner membrane space. Deletions in either oxa1 or cox18 result in yeast strains unable to respire. Over-expression of OXA1 does not allow for respiration in strains with deletions in cox18, but does allow for export of the C-terminus of Cox2. This suggests that Cox18 also has a role in the assembly of Cox2 into functional cytochrome c oxidase following the translocation of the C-terminus. I identified a physical interaction between Cox18 and the noncanonical chaperone Cox20, which is dependent on the presence of the Cox2 peptide. I isolated spontaneous respiring pseudorevertants from cox18 deletion strains carrying OXA1 on a high copy plasmid. Several of these pseudorevertants have mutations in residue 291 of the plasmid borne Oxa1. Substitution of charged residues, both positive and negative, at position 291 results in OXA1 alleles that can compensate for a deletion in cox18 when present in high copy number or when expressed from the chromosomal locus. Neutral substitutions at residue 291 of Oxa1 result in alleles that fail to compliment cox18 when present in high copy. These results show that the presence of a charged residue at position 291 allows Oxa1 to assemble Cox2 independent of Cox18 and its partner Mss2. The mutant alleles all retain Oxa1 function to various degrees. In addition, the alleles with charged residues compensate for a mss2 deletion. Cox20 is not required for assembly by these mutant proteins, although Cox20's chaperoning of Cox2 processing is required. Therefore, these mutant alleles are not simply replacing Cox18 in its normal complexes. Rather, they may interact with the Cox2 peptide in a novel way that makes it competent for assembly. Advisors/Committee Members: Fox, Thomas D. (chair), Hanson, Maureen R (committee member), Pleiss, Jeffrey A. (committee member).

Subjects/Keywords: cytochrome c oxidase; mitochondria; Cox20

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Elliott, L. (2013). Analysis Of The Assembly Of Saccharomyces Cerevisiae Cytochrome C Oxidase Subunit Cox2 By The Oxa1 And Cox18 Translocases. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/34300

Chicago Manual of Style (16th Edition):

Elliott, Leah. “Analysis Of The Assembly Of Saccharomyces Cerevisiae Cytochrome C Oxidase Subunit Cox2 By The Oxa1 And Cox18 Translocases.” 2013. Doctoral Dissertation, Cornell University. Accessed October 30, 2020. http://hdl.handle.net/1813/34300.

MLA Handbook (7th Edition):

Elliott, Leah. “Analysis Of The Assembly Of Saccharomyces Cerevisiae Cytochrome C Oxidase Subunit Cox2 By The Oxa1 And Cox18 Translocases.” 2013. Web. 30 Oct 2020.

Vancouver:

Elliott L. Analysis Of The Assembly Of Saccharomyces Cerevisiae Cytochrome C Oxidase Subunit Cox2 By The Oxa1 And Cox18 Translocases. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2020 Oct 30]. Available from: http://hdl.handle.net/1813/34300.

Council of Science Editors:

Elliott L. Analysis Of The Assembly Of Saccharomyces Cerevisiae Cytochrome C Oxidase Subunit Cox2 By The Oxa1 And Cox18 Translocases. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34300


University of Utah

2. Ferris, Elliott Carter. The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly.

Degree: MS, Biochemistry, 2011, University of Utah

Proper assembly of Cytochrome c Oxidase (CcO) is vital to mitochondrial respiration. The metallation and incorporation of CcO subunit 2 (Cox2) are important assembly steps that remain poorly understood. The Cox2 assembly factor Cox20 may provide a unique window on CcO assembly. Cox20 is known to bind Cox2 before the latter is incorporated into the larger CcO assembly in S. cerevisiae. Conservation of Cox20 in higher organisms and the lethality of its deletion in Drosophila melanogaster may suggest Cox20 plays a conserved role in CcO assembly. In an attempt to understand the nature of the hypothesized conserved role of Cox20, conserved residues within Cox20 were mutated and the resulting mutants studied. Mutation of a conserved cysteine pair impacted mitochondrial respiration, but did not block CcO assembly. The mutant Cox20 C87A forms a mixed disulfide species. Identifying the partner molecule may shed light on the role of Cox20. The present study finds Cox20, a 23 kD protein, in high molecular weight complexes that are unlikely to be homo-oligomeric. Identifying any other proteins in these complexes may shed light on the role Cox20 plays in CcO assembly. To this end, Cox20 was purified and analyzed by mass spectrometry (MS) to identify any accompanying proteins. Small quantities of the known CcO assembly factors Mss2, Coa1, and Mss51 were identified.

Subjects/Keywords: Cox2; Cox20; Cyclooxygenase 2; Cyclooxygenases; Cytochrome oxidase; C oxidase assembly; Mitochondrial respiration; Cox2 assembly factor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ferris, E. C. (2011). The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/214/rec/2651

Chicago Manual of Style (16th Edition):

Ferris, Elliott Carter. “The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly.” 2011. Masters Thesis, University of Utah. Accessed October 30, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/214/rec/2651.

MLA Handbook (7th Edition):

Ferris, Elliott Carter. “The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly.” 2011. Web. 30 Oct 2020.

Vancouver:

Ferris EC. The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly. [Internet] [Masters thesis]. University of Utah; 2011. [cited 2020 Oct 30]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/214/rec/2651.

Council of Science Editors:

Ferris EC. The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly. [Masters Thesis]. University of Utah; 2011. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/214/rec/2651

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