subject:(Correlation spectroscopy)

NSYSU

1. Wang, Yen-sheng. The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution.

Degree: Master, Electro-Optical Engineering, 2008, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905

► Luminescent conjugated polymers are widely used in organic optoelectronics. The device is fabricated by spin coating the polymer solutions into thin films. It is important… (more)

Subjects/Keywords: MDMO-PPV; Fluorescence correlation spectroscopy (FCS)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Y. (2008). The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Yen-sheng. “The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution.” 2008. Thesis, NSYSU. Accessed January 16, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Yen-sheng. “The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution.” 2008. Web. 16 Jan 2021.

Vancouver:

Wang Y. The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution. [Internet] [Thesis]. NSYSU; 2008. [cited 2021 Jan 16]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang Y. The investigation of the relation between conformation and spectroscopic properties of MDMO-PPV dilute solution. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0826108-155905

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Di Sabatino, Stefano. Reduced density-matrix functional theory : correlation and spectroscopy : Théorie de la fonctionnelle de la matrice densité réduite : corrélation et spectroscopie.

Degree: Docteur es, Physique, 2015, Université Toulouse III – Paul Sabatier

URL: http://www.theses.fr/2015TOU30137

► Cette thèse traite de la description de la corrélation électronique et de la spectroscopie dans le cadre de la Théorie de la Fonctionnelle de la… (more)

▼ Cette thèse traite de la description de la corrélation électronique et de la spectroscopie dans le cadre de la Théorie de la Fonctionnelle de la Matrice Densité Réduite (RDMFT). Dans la RDMFT, les propriétés de l'état fondamental d'un système physique sont des fonctionnelles de la matrice densité à un corps. Plusieurs approximations à la corrélation électronique ont été proposées dans la littérature. Beaucoup d'entre elles peuvent être reliés au travail de Müller, qui en a proposé une similaire à l'approximation Hartree-Fock mais qui peut produire des nombres d'occupation fractionnaires. Cela n'est pas toujours suffisant, notamment dans les matériaux fortement corrélés. Par ailleurs, l'expression des observables du système en terme de la matrice densité n'est pas toujours connue. Tel est le cas, par exemple, pour la fonction spectrale, qui est liée aux spectres de photoémission. Dans ce cas, il y a des annulations d'erreur entre l'approximation à la corrélation électronique et l'approximation à l'observable, ce qui affaiblit la théorie. Dans cette thèse, nous recherchons des approximations plus précises en exploitant le lien entre les matrices densité et les fonctions de Green. Dans la première partie de la thèse, nous nous concentrons sur la fonction spectrale. En utilisant le modèle de Hubbard, qui peut être résolu exactement, nous analysons les approximations existantes à cette observable et nous soulignons leurs points faibles. Ensuite, à partir de sa définition en terme de la fonction de Green à un corps nous dérivons une expression pour la fonction spectrale qui dépend des nombres d'occupation naturels et d'une énergie efficace qui prend en compte toutes les excitations du système. Cette énergie efficace dépend de la matrice densité à un corps ainsi que des ordres supérieurs. Des approximations simples à cette énergie efficace donnent des spectres précis dans des systèmes modèles dans des régimes à la fois de faible et de forte corrélation. Pour illustrer notre méthode sur les matériaux réels, nous calculons le spectre de photoemission du NiO massif: notre méthode donne une image qualitativement correcte dans la phase antiferromagnétique et dans la phase paramagnétique, contrairement aux méthodes de champ moyen utilisés actuellement, qui donnent un métal dans le dernier cas. La deuxième partie de la thèse est plus explorative et traite des phénomènes dépendant du temps dans la RDMFT. En général, l'évolution temporelle des matrices densité est donnée par la hiérarchie des équations de Bogoliubov-Born-Green-Kirkwood-Yvon (BBGKY), dans lequel l'équation du mouvement de la matrice densité a n corps est donnée en termes de la matrice densité à n+1 corps. La première équation de la hiérarchie relie la matrice densité à un corps à la matrice densité à deux corps. La tâche difficile est de trouver des approximations à la matrice densité à deux corps. Les approximations existantes sont des extensions adiabatiques des approximations de l'état fondamental. Nous explorons cette question en examinant de nouvelles… Advisors/Committee Members: Romaniello, Pina (thesis director).

Subjects/Keywords: RDMFT; TDRDMFT; MBPT; Green's functions; Correlation; Spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Di Sabatino, S. (2015). Reduced density-matrix functional theory : correlation and spectroscopy : Théorie de la fonctionnelle de la matrice densité réduite : corrélation et spectroscopie. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2015TOU30137

Chicago Manual of Style (16^th Edition):

Di Sabatino, Stefano. “Reduced density-matrix functional theory : correlation and spectroscopy : Théorie de la fonctionnelle de la matrice densité réduite : corrélation et spectroscopie.” 2015. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed January 16, 2021. http://www.theses.fr/2015TOU30137.

MLA Handbook (7^th Edition):

Di Sabatino, Stefano. “Reduced density-matrix functional theory : correlation and spectroscopy : Théorie de la fonctionnelle de la matrice densité réduite : corrélation et spectroscopie.” 2015. Web. 16 Jan 2021.

Vancouver:

Di Sabatino S. Reduced density-matrix functional theory : correlation and spectroscopy : Théorie de la fonctionnelle de la matrice densité réduite : corrélation et spectroscopie. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2015. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2015TOU30137.

Council of Science Editors:

Di Sabatino S. Reduced density-matrix functional theory : correlation and spectroscopy : Théorie de la fonctionnelle de la matrice densité réduite : corrélation et spectroscopie. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2015. Available from: http://www.theses.fr/2015TOU30137

University of Minnesota

3. Warner, Rochelle. Molecular and mechanical manipulation of membrane domains in planar supported bilayers.

Degree: MS, Chemistry, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/191292

► Biomembranes are dynamic, two-dimensional fluids that actively participate in biological functions such as signaling, membrane trafficking, endocytosis and exocytosis. They are composed of thousands of… (more)

Subjects/Keywords: fluorescence correlation spectroscopy; membrane domains; optical trapping

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APA (6^th Edition):

Warner, R. (2015). Molecular and mechanical manipulation of membrane domains in planar supported bilayers. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191292

Chicago Manual of Style (16^th Edition):

Warner, Rochelle. “Molecular and mechanical manipulation of membrane domains in planar supported bilayers.” 2015. Masters Thesis, University of Minnesota. Accessed January 16, 2021. http://hdl.handle.net/11299/191292.

MLA Handbook (7^th Edition):

Warner, Rochelle. “Molecular and mechanical manipulation of membrane domains in planar supported bilayers.” 2015. Web. 16 Jan 2021.

Vancouver:

Warner R. Molecular and mechanical manipulation of membrane domains in planar supported bilayers. [Internet] [Masters thesis]. University of Minnesota; 2015. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/11299/191292.

Council of Science Editors:

Warner R. Molecular and mechanical manipulation of membrane domains in planar supported bilayers. [Masters Thesis]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/191292

4. Moustaoui, Hanane. Développement de nanovecteurs à base de nanoparticules d'or : Applications biomédicales : Development of nanovector based on gold nanoparticules : Biomedical applications.

Degree: Docteur es, Chimie, 2018, Sorbonne Paris Cité

URL: http://www.theses.fr/2018USPCD065

►

Le cancer est la première cause de décès en France, il est devenu un enjeu majeur de santé publique. De nombreux traitements existent déjà pour… (more)

▼

Le cancer est la première cause de décès en France, il est devenu un enjeu majeur de santé publique. De nombreux traitements existent déjà pour lutter contre cette maladie telle que la chimiothérapie. Cependant, ces techniques utilisent des produits toxiques provoquant des effets secondaires très importants. Dans ce contexte, les nanoparticules d’or (GNP) suscitent un intérêt considérable en raison de leurs propriétés physiques et chimiques. Elles sont donc considérées comme des agents prometteurs pour le traitement du cancer, notamment pour l’acheminement de médicaments jusqu’à la tumeur (nanovecteur), ou encore pour la thérapie photothermique. Le but de cette thèse repose sur le développement de différents nanovecteurs basés sur des GNP avec différentes chimies de surface et l’étude de leur interaction avec des biomolécules (protéines),pour une application dans le traitement du cancer. Dans un premier temps, deux nanovecteurs à base d’or, de polyéthylène glycol et de doxorubicine ((DOX) comme agent anticancéreux) ont été synthétisés suivant deux stratégies différentes. La première stratégie a consisté à greffer la DOX aux GNP entouré de PEG par couplage carbodiimide. La deuxième stratégie, quant à elle, a été réalisée en complexant la DOX avec des ions d’or avant de procéder à la réduction de ces ions en présence du polymère. Ces différentes synthèses ont été caractérisées par MET, absorption optique et spectroscopie Raman. Enfin, une étude de viabilité cellulaire a été réalisée permettant de comparer l’effet cytotoxique des deux nanovecteurs sur une lignée adénocarcinome pancréatique (PANC-1). Dans une seconde partie, une étude a été réalisée sur l’adsorption de trois protéines du sérum sanguin humain (l’albumine, la glycoprotéine et le lysozyme) sur des GNP citratées. Pour cela, nous avons choisi d’utiliser la spectroscopie de corrélation de diffusion comme outil permettant de quantifier l’augmentation du rayon hydrodynamique des GNP provoquée par l’adsorption des protéines. Cette mesure du rayon hydrodynamique a permis de montrer que chaque protéine présente une orientation spécifique, mais aussi une constante d’affinité et une coopérativité différentes avec la surface d’or des nanoparticules. Enfin, dans une dernière partie une étude a été réalisée sur les propriétés photothermiques des nanoparticules d’or citratées de différentes tailles et différentes formes. Dans cette étude, les résultats montrent que l’élévation de température va dépendre de la position de la résonance plasmon par rapport à la longueur d’onde d’excitation, mais aussi de la forme et de la chimie de surface. Ce travail a été réalisé afin de comprendre comment les effets thermiques libérés par les nanoparticules d’or peuvent aboutir à la destruction de cellules cancéreuses.

The ﬁght against cancer is a major challenge for public health, since cancer remains the leading cause of death in France. Conventional treatment like chemotherapy use very toxic products that induce large side eﬀects. In this context, Gold nanoparticles (GNP) are of…

Advisors/Committee Members: Spadavecchia, Jolanda (thesis director), Lamy de la Chapelle, Marc (thesis director).

Subjects/Keywords: Nanovecteur; Spectroscopie de corrélation; Nanovector; Correlation spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moustaoui, H. (2018). Développement de nanovecteurs à base de nanoparticules d'or : Applications biomédicales : Development of nanovector based on gold nanoparticules : Biomedical applications. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2018USPCD065

Chicago Manual of Style (16^th Edition):

Moustaoui, Hanane. “Développement de nanovecteurs à base de nanoparticules d'or : Applications biomédicales : Development of nanovector based on gold nanoparticules : Biomedical applications.” 2018. Doctoral Dissertation, Sorbonne Paris Cité. Accessed January 16, 2021. http://www.theses.fr/2018USPCD065.

MLA Handbook (7^th Edition):

Moustaoui, Hanane. “Développement de nanovecteurs à base de nanoparticules d'or : Applications biomédicales : Development of nanovector based on gold nanoparticules : Biomedical applications.” 2018. Web. 16 Jan 2021.

Vancouver:

Moustaoui H. Développement de nanovecteurs à base de nanoparticules d'or : Applications biomédicales : Development of nanovector based on gold nanoparticules : Biomedical applications. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2018. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2018USPCD065.

Council of Science Editors:

Moustaoui H. Développement de nanovecteurs à base de nanoparticules d'or : Applications biomédicales : Development of nanovector based on gold nanoparticules : Biomedical applications. [Doctoral Dissertation]. Sorbonne Paris Cité; 2018. Available from: http://www.theses.fr/2018USPCD065

5. Vanzini, Marco. Auxiliary systems for observables : dynamical local connector approximation for electron addition and removal spectra : Systèmes auxiliaires pour les observables : approximation du connecteur dynamique locale pour les spectres d'addition et d'émission d'électrons.

Degree: Docteur es, Physique de la matière condensée, 2018, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2018SACLX012

► Cette thèse propose une méthode théorique innovante pour l'étude des spectres d'excitation à un électron, mesurée par spectroscopie de photoémission directe et inverse.La plupart des… (more)

▼ Cette thèse propose une méthode théorique innovante pour l'étude des spectres d'excitation à un électron, mesurée par spectroscopie de photoémission directe et inverse.La plupart des calculs actuels au niveau de l’état de l’art reposent sur des fonctions de Green à plusieurs corps et des self-énergies complexes et non locales, évaluées spécifiquement pour chaque matériau. Même lorsque les spectres calculés sont en très bon accord avec les expériences, le coût de calcul est très important. La raison est que la méthode elle-même n'est pas efficace, car elle fournit beaucoup d'informations superflues qui ne sont pas nécessaires pour l'interprétation des données expérimentales.Dans cette thèse, nous proposons deux raccourcis par rapport à la méthode standard. Le premier est l'introduction d'un système auxiliaire qui cible, en principe, le spectre d'excitation du système réel. L'exemple type est la théorie de la fonctionnelle de la densité, pour lequel le système auxiliaire est le système de Kohn-Sham : elle reproduit exactement la densité du système réel par l'intermédiaire d'un potentiel réel et statique, le potentiel de Kohn-Sham. La théorie de la fonctionnelle de la densité est, cependant, une théorie de l'état fondamental, qui ne fournit que rarement des propriétés d'état excités : un exemple est le fameux problème de la sous-estimation de la bande interdite. Le potentiel que nous proposons (le potentiel spectral), local et dépendant de la fréquence, mais réelle, peut être considéré comme une généralisation dynamique du potentiel de Kohn-Sham qui donne en principe le spectre exact.Le deuxième raccourci est l'idée de calculer ce potentiel une fois pour toute dans un système modèle, le gaz d'électrons homogène, et de le tabuler. Pour étudier des matériaux réels, nous concevons un connecteur qui prescrit l'utilisation des résultats du gaz pour calculer les spectres électroniques.La première partie de la thèse traite de l'idée de systèmes auxiliaires, montrant le cadre général dans lequel ils peuvent être introduits et les équations qu'ils doivent satisfaire. Nous utilisons des modèles de Hubbard solubles exactement pour mieux comprendre le rôle du potentiel spectral ; en particulier, il est démontré que le potentiel peut être défini uniquement chaque fois que le spectre est non nul, et donne toujours les spectres attendus, même lorsque la partie imaginaire ou les contributions non locales de la self-énergie jouent un rôle de premier plan.Dans la deuxième partie de la thèse, nous nous concentrons sur les calculs pour les systèmes réels. Nous évaluons d'abord le potentiel spectral dans le gaz d'électrons homogène, puis l'importons dans le système auxiliaire pour évaluer le spectre d'excitation. Toute l’interdependence non triviale entre l'interaction électronique et l'inhomogénéité du système réel entre dans la forme du connecteur. Trouver une expression pour cela est le véritable défi de la procédure. Nous proposons une approximation raisonnable basée sur les propriétés locales du système, que nous appelons approximation… Advisors/Committee Members: Gatti, Matteo (thesis director).

Subjects/Keywords: Spectroscopie théorique; Correlation; Approaches efficaces; Theoretical spectroscopy; Correlation; Effective approaches; 530.41

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vanzini, M. (2018). Auxiliary systems for observables : dynamical local connector approximation for electron addition and removal spectra : Systèmes auxiliaires pour les observables : approximation du connecteur dynamique locale pour les spectres d'addition et d'émission d'électrons. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2018SACLX012

Chicago Manual of Style (16^th Edition):

Vanzini, Marco. “Auxiliary systems for observables : dynamical local connector approximation for electron addition and removal spectra : Systèmes auxiliaires pour les observables : approximation du connecteur dynamique locale pour les spectres d'addition et d'émission d'électrons.” 2018. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed January 16, 2021. http://www.theses.fr/2018SACLX012.

MLA Handbook (7^th Edition):

Vanzini, Marco. “Auxiliary systems for observables : dynamical local connector approximation for electron addition and removal spectra : Systèmes auxiliaires pour les observables : approximation du connecteur dynamique locale pour les spectres d'addition et d'émission d'électrons.” 2018. Web. 16 Jan 2021.

Vancouver:

Vanzini M. Auxiliary systems for observables : dynamical local connector approximation for electron addition and removal spectra : Systèmes auxiliaires pour les observables : approximation du connecteur dynamique locale pour les spectres d'addition et d'émission d'électrons. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2018. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2018SACLX012.

Council of Science Editors:

Vanzini M. Auxiliary systems for observables : dynamical local connector approximation for electron addition and removal spectra : Systèmes auxiliaires pour les observables : approximation du connecteur dynamique locale pour les spectres d'addition et d'émission d'électrons. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2018. Available from: http://www.theses.fr/2018SACLX012

University of Alberta

6. Li, Xi. Aggregation of α-synuclein monomers and engineered oligomers in solution.

Degree: MS, Department of Chemistry, 2015, University of Alberta

URL: https://era.library.ualberta.ca/files/cgm80hv603

► α-Synuclein is a protein that has been found as fibrillar aggregates in Lewy bodies in the brain of Parkinson’s disease patients. Though the cause of… (more)

Subjects/Keywords: α-synuclein; Fluorescence correlation spectroscopy (FCS); Dual-color fluorescence cross-correlation spectroscopy (dual-color FCCS)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, X. (2015). Aggregation of α-synuclein monomers and engineered oligomers in solution. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cgm80hv603

Chicago Manual of Style (16^th Edition):

Li, Xi. “Aggregation of α-synuclein monomers and engineered oligomers in solution.” 2015. Masters Thesis, University of Alberta. Accessed January 16, 2021. https://era.library.ualberta.ca/files/cgm80hv603.

MLA Handbook (7^th Edition):

Li, Xi. “Aggregation of α-synuclein monomers and engineered oligomers in solution.” 2015. Web. 16 Jan 2021.

Vancouver:

Li X. Aggregation of α-synuclein monomers and engineered oligomers in solution. [Internet] [Masters thesis]. University of Alberta; 2015. [cited 2021 Jan 16]. Available from: https://era.library.ualberta.ca/files/cgm80hv603.

Council of Science Editors:

Li X. Aggregation of α-synuclein monomers and engineered oligomers in solution. [Masters Thesis]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/cgm80hv603

Cornell University

7. Iyer, Srikant. Development Of Bright Luminescent Silica Nanopartilces.

Degree: PhD, Chemistry and Chemical Biology, 2014, Cornell University

URL: http://hdl.handle.net/1813/36173

► Silica based luminescent nanoparticles provide tremendous potential as biocompatible and robust inorganic materials in nanobiotechnology and nanomedicine. Sol-gel derived silica act as excellent host for… (more)

Subjects/Keywords: Multiplexing; Chemiluminescence; Fluorescence correlation spectroscopy; Transient Absorption Spectroscopy

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APA (6^th Edition):

Iyer, S. (2014). Development Of Bright Luminescent Silica Nanopartilces. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/36173

Chicago Manual of Style (16^th Edition):

Iyer, Srikant. “Development Of Bright Luminescent Silica Nanopartilces.” 2014. Doctoral Dissertation, Cornell University. Accessed January 16, 2021. http://hdl.handle.net/1813/36173.

MLA Handbook (7^th Edition):

Iyer, Srikant. “Development Of Bright Luminescent Silica Nanopartilces.” 2014. Web. 16 Jan 2021.

Vancouver:

Iyer S. Development Of Bright Luminescent Silica Nanopartilces. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1813/36173.

Council of Science Editors:

Iyer S. Development Of Bright Luminescent Silica Nanopartilces. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36173

University of Oxford

8. Nolan, Rory. Algorithms for the correction of photobleaching.

Degree: PhD, 2018, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417

► The measured intensity (ideally in units of photon counts) of a fluorescent sample over time constitutes a time-series called an intensity trace. The idea of… (more)

Subjects/Keywords: Fluorescence correlation spectroscopy; Fluorescence; HIV; Image Analysis; Microscopy; Fluorescence fluctuation spectroscopy; Spectroscopy

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APA (6^th Edition):

Nolan, R. (2018). Algorithms for the correction of photobleaching. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417

Chicago Manual of Style (16^th Edition):

Nolan, Rory. “Algorithms for the correction of photobleaching.” 2018. Doctoral Dissertation, University of Oxford. Accessed January 16, 2021. http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417.

MLA Handbook (7^th Edition):

Nolan, Rory. “Algorithms for the correction of photobleaching.” 2018. Web. 16 Jan 2021.

Vancouver:

Nolan R. Algorithms for the correction of photobleaching. [Internet] [Doctoral dissertation]. University of Oxford; 2018. [cited 2021 Jan 16]. Available from: http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417.

Council of Science Editors:

Nolan R. Algorithms for the correction of photobleaching. [Doctoral Dissertation]. University of Oxford; 2018. Available from: http://ora.ox.ac.uk/objects/uuid:551b46cb-a266-44c6-8f6d-66e49b7161bc ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.770417

University of Akron

9. Klufas, Megan J. Resolving Membrane Receptor Multimerization in Live Cells using Time Resolved Fluorescence Methods.

Degree: PhD, Chemistry, 2017, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron151017994353956

► The cell membrane is a complex environment made up of thousands of molecular components. The dynamic assembly of these components regulates a myriad of cellular… (more)

▼ The cell membrane is a complex environment made up of thousands of molecular components. The dynamic assembly of these components regulates a myriad of cellular functions, but it is difficult to measure in a biologically relevant context. Pulsed interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS) is a time resolved fluorescence technique that was used to obtain concentration, mobility and co-diffusion (f_c) of membrane proteins in live cells. Several lines of evidence support the hypothesis that homo-dimerization (or even oligomerization) facilitates the function of membrane proteins. The goal of this research was to elucidate the dynamic organization and relative affinity of membrane protein-protein interactions. In order to accomplish this goal, a mathematical model was developed to interpret the cross-correlation value obtained from PIE-FCCS and to quantify the dynamic interactions of membrane receptors in a more rigorous way.This thesis describes three main projects. The first project focused on determining the homo-dimerization of the neuronal membrane protein PlexinA4 before and after ligand stimulation. PIE-FCCS measurements of protein controls with varying degrees of homo-multimerization were used to determine that PlexinA4 receptors assemble into preformed dimers. In the next project, a mathematical model was developed to interpret the PIE-FCCS figure of merit, f_c. Several controls systems with varying dimerization affinity and degrees of oligomerization were measured and analyzed to verify the accuracy of the model. Lastly, the organization of two class A G protein-coupled receptors (GPCRs) was investigated using PIE-FCCS and a new labeling strategy. The organization of these membrane proteins showed a high degree of cell to cell variability. A simple monomer-dimer equilibrium model failed to describe the range of single cell data, so the mathematical model was altered to describe a cluster model that agreed with the experimental data. This thesis lays the groundwork for application of the mathematical model to quantify the organization of other membrane receptors using PIE-FCCS. Advisors/Committee Members: Smith, Adam (Advisor).

Subjects/Keywords: Biochemistry; Biophysics; Chemistry; fluorescence correlation spectroscopy; fluorescence cross-correlation spectroscopy; pulsed interleaved excitation; homodimerization; homo-oligomerization; membrane organization; protein-protein interactions

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APA (6^th Edition):

Klufas, M. J. (2017). Resolving Membrane Receptor Multimerization in Live Cells using Time Resolved Fluorescence Methods. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron151017994353956

Chicago Manual of Style (16^th Edition):

Klufas, Megan J. “Resolving Membrane Receptor Multimerization in Live Cells using Time Resolved Fluorescence Methods.” 2017. Doctoral Dissertation, University of Akron. Accessed January 16, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron151017994353956.

MLA Handbook (7^th Edition):

Klufas, Megan J. “Resolving Membrane Receptor Multimerization in Live Cells using Time Resolved Fluorescence Methods.” 2017. Web. 16 Jan 2021.

Vancouver:

Klufas MJ. Resolving Membrane Receptor Multimerization in Live Cells using Time Resolved Fluorescence Methods. [Internet] [Doctoral dissertation]. University of Akron; 2017. [cited 2021 Jan 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron151017994353956.

Council of Science Editors:

Klufas MJ. Resolving Membrane Receptor Multimerization in Live Cells using Time Resolved Fluorescence Methods. [Doctoral Dissertation]. University of Akron; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron151017994353956

Colorado State University

10. Abdollah-Nia, Farshad. Probing molecular kinetics using higher-order fluorescence correlation spectroscopy.

Degree: PhD, Physics, 2019, Colorado State University

URL: http://hdl.handle.net/10217/195278

► Fluorescence correlation spectroscopy (FCS) is a powerful tool in the time-resolved analysis of non-reacting or reacting molecules in solution, based on fluorescence intensity fluctuations. However,… (more)

▼ Fluorescence correlation spectroscopy (FCS) is a powerful tool in the time-resolved analysis of non-reacting or reacting molecules in solution, based on fluorescence intensity fluctuations. However, conventional (second-order) FCS alone is insufficient to measure all parameters needed to describe a reaction or mixture, including concentrations, fluorescence brightnesses, and forward and reverse rate constants. For this purpose, correlations of higher powers of fluorescence intensity fluctuations can be calculated to yield additional information from the single-photon data stream collected in an FCS experiment. To describe systems of diffusing and reacting molecules, considering cumulants of fluorescence intensity results in simple expressions in which the reaction and the diffusion parts factorize. The computation of higher-order correlations in experiments is hindered by shot noise and common detector artifacts, the effects of which become worse with increasing order. We introduce a technique to calculate artifact-free higher-order correlation functions with improved time resolution, and without any need for modeling and calibration of detector artifacts. The technique is formulated for general multi-detector experiments and verified in both two-detector and single-detector configurations. Good signal-to-noise ratio is achieved down to 1 μs in correlation curves up to order (2,2). Next, we demonstrate applications of the technique to analyze systems of fast and slow reactions. As an example of slow- or non-reacting systems, the technique is applied to resolve two-component mixtures of labeled oligonucleotides. Then, the protonation reaction of fluorescein isothiocyanate (FITC) in phosphate buffer is analyzed as an example of fast reactions (relaxation time < 10 μs). By reference to an (apparent) non-reacting system, the simple factorized form of cumulant-based higher-order correlations is exploited to remove the dependence on the molecular detection function (MDF). Therefore, there is no need to model and characterize the experimental MDF, and the precision and the accuracy of the technique are enhanced. It is verified that higher-order correlation analysis enables complete and simultaneous determination of number and brightness parameters of mixing or reacting molecules, the reaction relaxation time, and forward and reverse reaction rates. Finally, we apply the technique to analyze the conformational dynamics of DNA hairpins. Previous FCS measurements of DNA hairpin folding dynamics revealed at least three conformational states of the DNA are present, distinguished by the brightness of fluorescent dye-quencher labels. Rapid fluctuations between two of the states occurred on time scales observable by FCS. A third state that was static on the FCS time scale was also observed. We show that conventional FCS alone cannot uniquely distinguish the conformational states or assign their roles in the observed mechanism. The additional information offered by higher-order FCS makes it possible (i) to uniquely identify the… Advisors/Committee Members: Gelfand, Martin P. (advisor), Van Orden, Alan (advisor), Krapf, Diego (committee member), Prasad, Ashok (committee member), Roberts, Jacob (committee member).

Subjects/Keywords: high-order fluorescence correlation spectroscopy; nucleic acids; higher-order fluorescence correlation spectroscopy; conformational dynamics; molecular kinetics in solution; DNA

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APA (6^th Edition):

Abdollah-Nia, F. (2019). Probing molecular kinetics using higher-order fluorescence correlation spectroscopy. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/195278

Chicago Manual of Style (16^th Edition):

Abdollah-Nia, Farshad. “Probing molecular kinetics using higher-order fluorescence correlation spectroscopy.” 2019. Doctoral Dissertation, Colorado State University. Accessed January 16, 2021. http://hdl.handle.net/10217/195278.

MLA Handbook (7^th Edition):

Abdollah-Nia, Farshad. “Probing molecular kinetics using higher-order fluorescence correlation spectroscopy.” 2019. Web. 16 Jan 2021.

Vancouver:

Abdollah-Nia F. Probing molecular kinetics using higher-order fluorescence correlation spectroscopy. [Internet] [Doctoral dissertation]. Colorado State University; 2019. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10217/195278.

Council of Science Editors:

Abdollah-Nia F. Probing molecular kinetics using higher-order fluorescence correlation spectroscopy. [Doctoral Dissertation]. Colorado State University; 2019. Available from: http://hdl.handle.net/10217/195278

University of Pennsylvania

11. Kim, Meeri Nam. Applications of Hybrid Diffuse Optics for Clinical Management of Adults After Brain injury.

Degree: 2013, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/768

► Information about cerebral blood flow (CBF) is valuable for clinical management of patients after severe brain injury. Unfortunately, current modalities for monitoring brain are often… (more)

▼ Information about cerebral blood flow (CBF) is valuable for clinical management of patients after severe brain injury. Unfortunately, current modalities for monitoring brain are often limited by hurdles that include high cost, low throughput, exposure to ionizing radiation, probe invasiveness, and increased risk to critically ill patients when transportation out of their room or unit is required. A further limitation of current technologies is an inability to provide continuous bedside measurements that are often desirable for unstable patients. Here we explore the clinical utility of diffuse correlation spectroscopy (DCS) as an alternative approach for bedside CBF monitoring. DCS uses the rapid intensity fluctuations of near-infrared light to derive a continuous measure of changes in blood flow without ionizing radiation or invasive probing. Concurrently, we employ another optical technique, called diffuse optical spectroscopy (DOS), to derive changes in cerebral oxyhemoglobin (HbO2) and deoxyhemoglobin (Hb) concentrations. Our clinical studies integrate DCS with DOS into a single hybrid instrument that simultaneously monitors CBF and HbO2/Hb in the injured adult brain. The first parts of this dissertation present the motivations for monitoring blood flow in injured brain, as well as the theory underlying diffuse optics technology. The next section elaborates on details of the hybrid instrumentation. The final chapters describe four human subject studies carried out with these methods. Each of these studies investigates an aspect of the potential of the hybrid monitor in clinical applications involving adult brain. The studies include: (1) validation of DCS-measured CBF against xenon-enhanced computed tomography in brain-injured adults; (2) a study of the effects of age and gender on posture-change-induced CBF variation in healthy subjects; (3) a study of the efficacy of DCS/DOS for monitoring neurocritical care patients during various medical interventions such as head-of-bed manipulation and induced hyperoxia; and (4) a first feasibility study for using DCS to study hemodynamics at high altitudes. The work presented in this dissertation thus further develops DCS/DOS technology and demonstrates its utility for monitoring the injured adult brain. It demonstrates the promise of this new clinical tool to help neurocritical care clinicians make more informed decisions and thereby improve patient outcome.

Subjects/Keywords: diffuse correlation spectroscopy; medical physics; near infrared spectroscopy; optics; physics; Physics; Radiology

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APA (6^th Edition):

Kim, M. N. (2013). Applications of Hybrid Diffuse Optics for Clinical Management of Adults After Brain injury. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/768

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kim, Meeri Nam. “Applications of Hybrid Diffuse Optics for Clinical Management of Adults After Brain injury.” 2013. Thesis, University of Pennsylvania. Accessed January 16, 2021. https://repository.upenn.edu/edissertations/768.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kim, Meeri Nam. “Applications of Hybrid Diffuse Optics for Clinical Management of Adults After Brain injury.” 2013. Web. 16 Jan 2021.

Vancouver:

Kim MN. Applications of Hybrid Diffuse Optics for Clinical Management of Adults After Brain injury. [Internet] [Thesis]. University of Pennsylvania; 2013. [cited 2021 Jan 16]. Available from: https://repository.upenn.edu/edissertations/768.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kim MN. Applications of Hybrid Diffuse Optics for Clinical Management of Adults After Brain injury. [Thesis]. University of Pennsylvania; 2013. Available from: https://repository.upenn.edu/edissertations/768

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

12. Sly, Krystal Lynne. Investigation of biomolecular interactions at the surface of lipid bilayers using second harmonic generation spectroscopy, lens-less imaging and correlation spectroscopy.

Degree: PhD, Chemistry, 2014, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3324/rec/1420

► This dissertation focuses on the study of surface biomolecular interactions using second harmonic generation (SHG) spectroscopy, surface SHG imaging (SSHGI), and SH correlation spectroscopy (SHCS).… (more)

▼ This dissertation focuses on the study of surface biomolecular interactions using second harmonic generation (SHG) spectroscopy, surface SHG imaging (SSHGI), and SH correlation spectroscopy (SHCS). The binding kinetics and energetics of four biotinboundproteins, avidin, streptavidin, neutrAvidin, and anti-biotin antibody were compared and data revealed significant differences in their apparent binding affinities andnonspecific binding. Specifically, protein-protein interactions were found to play an important role in the apparent binding affinity, making the streptavidin-biotin interaction the most energetically favorable. The details of the binding properties of these frequentlyemployed tether/linker protein-biotin complexes provide valuable information for biosensors, immunoassays, and medical diagnostics.As most biosensor platforms are designed for high throughput detection, the resolution and planar wave-front of the SSHGI system was thoroughly analyzed. It was demonstrated that the coherent plane wave generated by SHG followed Gaussian beampropagation, enabling SSHGI to image without a lens system at rather long distances. Lens-less imaging simplifies the detection method, increases photon collection efficiency, and increases the detection area. These advantages could potentially makeSSHGI a simple, label-free high throughput detection method for surface biomolecular interactions. The versatility and sensitivity of SHG were further probed by coupling SHG with correlation spectroscopy, a statistical fluctuation time-dependent method. SHCS wasestablished as a viable and valuable option for the detection of surface binding kinetics for small molecule and protein-ligand interactions at the surface of lipid bilayers. First, the simple binding kinetics of a small molecule, (s)-(+)-1,1’-bi-2-napthol (SBN),incorporating into a lipid bilayer was determined using SHCS and results were statistically similar to those obtained from a traditional binding isotherm. Next, SHCS was used to examine the binding kinetics of a more complex interaction between the multivalent proteins, cholera toxin subunit b (CTb) and peanut agglutinin (PnA), and a GM1 doped lipid bilayer. SHCS was able to obtain the binding kinetics for these surfacebiomolecular interactions with more efficiency, less analyte, and less sensitivity to mass transport effects. Cumulatively, the studies of this dissertation showcase SHG, SSHGI, and SHCS as valuable label-free detection methods with incredible sensitivity for investigation ofsurface biomolecular interactions.

Subjects/Keywords: Lens-less imaging; Protein-ligand binding; Second harmonic correlation spectroscopy; SHG

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APA (6^th Edition):

Sly, K. L. (2014). Investigation of biomolecular interactions at the surface of lipid bilayers using second harmonic generation spectroscopy, lens-less imaging and correlation spectroscopy. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3324/rec/1420

Chicago Manual of Style (16^th Edition):

Sly, Krystal Lynne. “Investigation of biomolecular interactions at the surface of lipid bilayers using second harmonic generation spectroscopy, lens-less imaging and correlation spectroscopy.” 2014. Doctoral Dissertation, University of Utah. Accessed January 16, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3324/rec/1420.

MLA Handbook (7^th Edition):

Sly, Krystal Lynne. “Investigation of biomolecular interactions at the surface of lipid bilayers using second harmonic generation spectroscopy, lens-less imaging and correlation spectroscopy.” 2014. Web. 16 Jan 2021.

Vancouver:

Sly KL. Investigation of biomolecular interactions at the surface of lipid bilayers using second harmonic generation spectroscopy, lens-less imaging and correlation spectroscopy. [Internet] [Doctoral dissertation]. University of Utah; 2014. [cited 2021 Jan 16]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3324/rec/1420.

Council of Science Editors:

Sly KL. Investigation of biomolecular interactions at the surface of lipid bilayers using second harmonic generation spectroscopy, lens-less imaging and correlation spectroscopy. [Doctoral Dissertation]. University of Utah; 2014. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3324/rec/1420

13. Ghaderi, Tuana. X-ray intensity correlation spectroscopy from fluid surfaces.

Degree: 2006, Technische Universität Dortmund

URL: http://hdl.handle.net/2003/22293

► X-ray intensity correlation spectroscopy (XICS) is a coherent X-ray scattering technique, which enables the investigation of dynamic properties of matter by analyzing the temporal correlations… (more)

▼ X-ray intensity correlation spectroscopy (XICS) is a coherent X-ray scattering technique, which enables the investigation of dynamic properties of matter by analyzing the temporal correlations among intensities scattered by the studied material. This novel technique has been intensively applied in the last decade in order to examine the temporal and lateral correlation properties of fluid surfaces. Although, intensity correlation experiments are qualitatively well understood, present theoretical interpretations fail to explain XICS data from some well known fluid surfaces, such as water and glycerol. We believe that the discrepancies, between the theoretical predictions for the intensity correlation function and the experimental results, are due to some idealized assumptions with regard to the coherence of the X-ray beam, as well as the instrumental resolution. This thesis is mainly concerned with the derivation of the intensity correlation function for surface sensitive X-ray intensity correlation experiments including the effects of partial coherence and instrumental resolution. In order to derive the intensity correlation function the theoretical approach is based in this work on the statistical properties of the fluid surface and the scattered electric field. A scalar wave equation for the electric X-ray field is derived to determine the field expressions from time fluctuating and inhomogeneous media. The therefrom obtained field formulas are used to derive systematically field correlation functions and intensity correlation functions. The accuracy of the field expressions and the deduced correlation functions are restricted to the first Born approximation. Within this accuracy, we have provided intensity correlation functions that are applicable to charge scattering from fluid surfaces under the conditions of arbitrary spatial coherence and instrumental resolution. In addition, far and near field scattering conditions, i.e. Fraunhofer and Fresnel conditions, are rigorously incorporated in the theoretical intensity correlation functions. The experimental part in thesis is dedicated to the analysis of XICS measurements from hexane and water surfaces. The data analysis is based in parts on the intensity correlation function which is derived in the theoretical part of this work. We have convincingly illustrated that the conventionally used intensity correlation function is generally not suitable to analyze XICS experiments. In contrast to the conventionally used intensity correlation formula, we have obtained very good agreement between our theoretical intensity correlation function and the experimental results from hexane. This work may therefore be of general interest to scientists who make use of XICS or other scattering techniques using partially coherent X-ray beams. Advisors/Committee Members: Tolan, Metin.

Subjects/Keywords: Coherent x-ray scattering; Correlation spectroscopy; XICS; XPCS; X-ray; 530

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APA (6^th Edition):

Ghaderi, T. (2006). X-ray intensity correlation spectroscopy from fluid surfaces. (Thesis). Technische Universität Dortmund. Retrieved from http://hdl.handle.net/2003/22293

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ghaderi, Tuana. “X-ray intensity correlation spectroscopy from fluid surfaces.” 2006. Thesis, Technische Universität Dortmund. Accessed January 16, 2021. http://hdl.handle.net/2003/22293.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ghaderi, Tuana. “X-ray intensity correlation spectroscopy from fluid surfaces.” 2006. Web. 16 Jan 2021.

Vancouver:

Ghaderi T. X-ray intensity correlation spectroscopy from fluid surfaces. [Internet] [Thesis]. Technische Universität Dortmund; 2006. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2003/22293.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ghaderi T. X-ray intensity correlation spectroscopy from fluid surfaces. [Thesis]. Technische Universität Dortmund; 2006. Available from: http://hdl.handle.net/2003/22293

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

14. Reid, Kemar Ricardo. Unraveling the Interplay between Structure and Photophysics in Colloidal Quantum Dot Nanostructures.

Degree: PhD, Interdisciplinary Materials Science, 2019, Vanderbilt University

URL: http://hdl.handle.net/1803/10698

► Colloidal quantum dots (QDs) make up a class of nanomaterials that provide a remarkable platform for engineering photophysical properties tailored to a range of applications… (more)

▼ Colloidal quantum dots (QDs) make up a class of nanomaterials that provide a remarkable platform for engineering photophysical properties tailored to a range of applications of considerable technological value, including energy-efficient LEDs, robust biological probes, low-threshold solution-processed lasers, advanced photocatalysts and single-photon sources for quantum technologies. Underpinning these uses of QDs lay more fundamental investigations into the role of structure on absorption, energy relaxation, charge separation and charge recombination in order to achieve complete control over their photophysical behavior. However, with complex interfaces and diverging morphologies in synthetic products, QDs are remarkably complex nanostructured materials. It is therefore challenging to develop detailed structure-function relationships at the ensemble scale—where the properties of an array of structures are simultaneously determined. To decode this information, it is necessary to study QD nanostructures one at a time. In this work, single QD investigations were applied to three different QD systems to better understand the interplay between structure and photophysics in these materials. From revealing the role of strain-induced structural changes on the color purity of CdSe-CdS core-shell QDs, to a thick-shell in suppressing random intermittent emission in cadmium-free InP QD nanostructures, these investigations continually point to the negative impact of structural heterogeneity on the properties of the ensemble. The implementation of a correlative technique to measure the structure and photophysics of the same QD facilitated a detailed examination of this heterogeneity in semiconductor nanorods—revealing the detrimental effects of surface roughness on the quantum efficiency of the material. The advanced characterization of QDs afforded by this technique also has far-reaching implications for QD development and could be used to inform methods to orchestrate the assembly of QD nanostructures with precisely tuned photophysical properties. Advisors/Committee Members: Jason G. Valentine (committee member), Janet E. Macdonald (committee member), Richard F. Haglund (committee member), Rizia Bardhan (committee member), Sandra J. Rosenthal (Committee Chair).

Subjects/Keywords: photophysics; atomic structure; electron microscopy; optical spectroscopy; correlation; quantum dot

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APA (6^th Edition):

Reid, K. R. (2019). Unraveling the Interplay between Structure and Photophysics in Colloidal Quantum Dot Nanostructures. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10698

Chicago Manual of Style (16^th Edition):

Reid, Kemar Ricardo. “Unraveling the Interplay between Structure and Photophysics in Colloidal Quantum Dot Nanostructures.” 2019. Doctoral Dissertation, Vanderbilt University. Accessed January 16, 2021. http://hdl.handle.net/1803/10698.

MLA Handbook (7^th Edition):

Reid, Kemar Ricardo. “Unraveling the Interplay between Structure and Photophysics in Colloidal Quantum Dot Nanostructures.” 2019. Web. 16 Jan 2021.

Vancouver:

Reid KR. Unraveling the Interplay between Structure and Photophysics in Colloidal Quantum Dot Nanostructures. [Internet] [Doctoral dissertation]. Vanderbilt University; 2019. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1803/10698.

Council of Science Editors:

Reid KR. Unraveling the Interplay between Structure and Photophysics in Colloidal Quantum Dot Nanostructures. [Doctoral Dissertation]. Vanderbilt University; 2019. Available from: http://hdl.handle.net/1803/10698

Texas A&M University

15. Hong, Sung Min. Microfluidics for Single Molecule Detection and Material Processing.

Degree: PhD, Electrical Engineering, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11726

► In the cancer research, it is important to understand protein dynamics which are involved in cell signaling. Therefore, particular protein detection and analysis of target… (more)

▼ In the cancer research, it is important to understand protein dynamics which are involved in cell signaling. Therefore, particular protein detection and analysis of target protein behavior are indispensable for current basic cancer research. However, it usually performed by conventional biochemical approaches, which require long process time and a large amount of samples. We have been developed the new applications based on microfluidics and Raster image Correlation spectroscopy (RICS) techniques. A simple microfluidic 3D hydrodynamic flow focusing device has been developed for quantitative determinations of target protein concentrations. The analyte stream was pinched not only horizontally, but also vertically by two sheath streams by introducing step depth cross junction structure. As a result, a triangular cross-sectional flow profile was formed and the laser was focused on the top of the triangular shaped analyte stream. Through this approach, the target protein concentration was successfully determined in cell lysate samples. The RICS technique has been applied to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. P53 tagged with Green Fluores-cent Protein (GFP) were incubated with and without DNA damaging agents, cisplatin or eptoposide. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. This result demonstrates that RICS is able to detect protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening. As another application of microfluidics, an integrated microfluidic platform was developed for generating collagen microspheres with encapsulation of viable cells. The platform integrated four automated functions on a microfluidic chip, (1) collagen solution cooling system, (2) cell-in-collagen microdroplet generation, (3) collagen microdroplet polymerization, and (4) incubation and extraction of the microspheres. This platform provided a high throughput and easy way to generate uniform dimensions of collagen microspheres encapsulating viable cells that were able to proliferate for more than 1 week. Advisors/Committee Members: Jun, Kameoka (advisor), Su, Chin B. (committee member), Righetti, Raffaella (committee member), Chang, Kuang-An (committee member).

Subjects/Keywords: Microfluidics; Single Molecule Detection; Raster Image Correlation Spectroscopy; Collagen Microspheres

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APA (6^th Edition):

Hong, S. M. (2012). Microfluidics for Single Molecule Detection and Material Processing. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11726

Chicago Manual of Style (16^th Edition):

Hong, Sung Min. “Microfluidics for Single Molecule Detection and Material Processing.” 2012. Doctoral Dissertation, Texas A&M University. Accessed January 16, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11726.

MLA Handbook (7^th Edition):

Hong, Sung Min. “Microfluidics for Single Molecule Detection and Material Processing.” 2012. Web. 16 Jan 2021.

Vancouver:

Hong SM. Microfluidics for Single Molecule Detection and Material Processing. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11726.

Council of Science Editors:

Hong SM. Microfluidics for Single Molecule Detection and Material Processing. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11726

Rice University

16. Daniels, Charlisa. Monitoring Transport at Interfaces of Tunable Soft Surfaces.

Degree: PhD, Natural Sciences, 2012, Rice University

URL: http://hdl.handle.net/1911/64638

► The present work utilizes single molecule methods and analysis to investigate soft and hard substrates. First, the effect of charged hard surfaces on charged probes… (more)

Subjects/Keywords: Diffusion; Fluorescence correlation spectroscopy; Polymerized Surfaces; Physical chemistry

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APA (6^th Edition):

Daniels, C. (2012). Monitoring Transport at Interfaces of Tunable Soft Surfaces. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/64638

Chicago Manual of Style (16^th Edition):

Daniels, Charlisa. “Monitoring Transport at Interfaces of Tunable Soft Surfaces.” 2012. Doctoral Dissertation, Rice University. Accessed January 16, 2021. http://hdl.handle.net/1911/64638.

MLA Handbook (7^th Edition):

Daniels, Charlisa. “Monitoring Transport at Interfaces of Tunable Soft Surfaces.” 2012. Web. 16 Jan 2021.

Vancouver:

Daniels C. Monitoring Transport at Interfaces of Tunable Soft Surfaces. [Internet] [Doctoral dissertation]. Rice University; 2012. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1911/64638.

Council of Science Editors:

Daniels C. Monitoring Transport at Interfaces of Tunable Soft Surfaces. [Doctoral Dissertation]. Rice University; 2012. Available from: http://hdl.handle.net/1911/64638

Rice University

17. Tcherniak, Alexei. Development and Application of Methods to Study Nanoparticle Diffusion Using Intensity Correlation Spectroscopy.

Degree: PhD, Natural Sciences, 2011, Rice University

URL: http://hdl.handle.net/1911/70472

► The practical application of nanoparticles requires transitioning from well controlled experimental settings to highly variable "real-life" conditions. Understanding the resulting changes in the behavior and… (more)

Subjects/Keywords: Pure sciences; Nanoparticle diffusion; Correlation spectroscopy; Physical chemistry

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APA (6^th Edition):

Tcherniak, A. (2011). Development and Application of Methods to Study Nanoparticle Diffusion Using Intensity Correlation Spectroscopy. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/70472

Chicago Manual of Style (16^th Edition):

Tcherniak, Alexei. “Development and Application of Methods to Study Nanoparticle Diffusion Using Intensity Correlation Spectroscopy.” 2011. Doctoral Dissertation, Rice University. Accessed January 16, 2021. http://hdl.handle.net/1911/70472.

MLA Handbook (7^th Edition):

Tcherniak, Alexei. “Development and Application of Methods to Study Nanoparticle Diffusion Using Intensity Correlation Spectroscopy.” 2011. Web. 16 Jan 2021.

Vancouver:

Tcherniak A. Development and Application of Methods to Study Nanoparticle Diffusion Using Intensity Correlation Spectroscopy. [Internet] [Doctoral dissertation]. Rice University; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1911/70472.

Council of Science Editors:

Tcherniak A. Development and Application of Methods to Study Nanoparticle Diffusion Using Intensity Correlation Spectroscopy. [Doctoral Dissertation]. Rice University; 2011. Available from: http://hdl.handle.net/1911/70472

University of Illinois – Urbana-Champaign

18. Jacob, Lisa. Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy.

Degree: MS, Chemistry, 2015, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/89156

► When a nanoparticle enters a biological environment, molecules are known to adsorb on the surface forming a corona. Systematically studying the formation of a corona… (more)

Subjects/Keywords: nano-bio interactions; fluorescence correlation spectroscopy; lipid corona

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APA (6^th Edition):

Jacob, L. (2015). Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/89156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jacob, Lisa. “Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy.” 2015. Thesis, University of Illinois – Urbana-Champaign. Accessed January 16, 2021. http://hdl.handle.net/2142/89156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jacob, Lisa. “Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy.” 2015. Web. 16 Jan 2021.

Vancouver:

Jacob L. Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2142/89156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jacob L. Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy. [Thesis]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/89156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Fenta, Abel Eduardo Silva. Locally probing adatoms on graphene using perturbed angular correlation spectroscopy .

Degree: 2019, Universidade de Aveiro

URL: http://hdl.handle.net/10773/28060

► The interest in understanding the interaction between graphene and atoms and molecules that are adsorbed on its surface (adatoms and admolecules) spans a wide range… (more)

▼ The interest in understanding the interaction between graphene and atoms and molecules that are adsorbed on its surface (adatoms and admolecules) spans a wide range of research ﬁelds and applications, for example, to controllably change the properties of graphene in electronic devices or to detect those changes in graphene-based sensors. Since the properties of graphene+adsorbent systems are strongly dependent on the adsorption conﬁguration (e.g. isolated versus clusters, their geometry and coordination), it is important to not only understand these eﬀects from a theoretical point of view, but also to be able to probe them experimentally. This thesis focuses on the interaction between graphene and adatoms (model cases: Hg, Cd, In and Ag) and admolecules (model case: HgO2), in terms of structural and electronic properties. The theoretical part of the research was based on density functional theory (DFT) calculations for diﬀerent heavy elements adsorbed on graphene (Hg, Ag, Cd and In). The binding energy and the electronic structure were calculated for various high-symmetry atomic conﬁgurations, from isolated adatoms to a continuous monolayer. Detailed studies were carried out for Hg-graphene, as a model system, i.e. testing various functionals and covering a wide range of nominal concentration. For Ag, Cd, In, we carried out more targeted studies, based on the extensive calculations for Hg. It was demonstrate that the binding energy depends on adatom concentration and as well on adsorption site (H, T, or B). All studied elements are predicted to be more stable as isolated adatoms than as a continuous monolayer. For each element, we calculated the electric ﬁeld gradient (EFG) parameters (Vzz and asymmetry parameter η). The EFG exhibits some variation when comparing diﬀerent high-symmetry sites, and also depends on the nominal concentration of the adatom relative to C atoms. Furthermore, the EFG is found to be sensitive to the local atomic structure, distinguishing isolated from monolayer conﬁgurations, and for some cases, varying signiﬁcantly with small variations in adatom position (at the sub-Å scale). Based on these calculations, we propose that the electric ﬁeld gradient, which can be measured using hyperﬁne techniques, can be used as an experimental observable providing insight on the local atomic conﬁguration and bonding stability of adatoms and admolecules on graphene. In particular, our calculations indicate that the level of detail that can be addressed via the EFG parameters (e.g. positional precision) increases with the stability (binding energy). In other words, the more stable the graphene-adatom system (i.e. more relevant in a application scenario), the more it lends itself to be studied using hyperﬁne techniques. Finally, adsorption of Hg on graphene in ambient conditions was experimentally studied using perturbed angular correlation (PAC) spectroscopy and 199mHg as probe nuclei. The combination of PAC measurements and DFT calculations allowed us to conclude that the majority of the Hg probes were… Advisors/Committee Members: Amaral, Vítor Brás de Sequeira (advisor), Pereira, Lino da Costa (advisor), Correia, João Guilherme Martins (advisor).

Subjects/Keywords: Graphene; Adatom; Admolecule; Perturbed angular correlation spectroscopy; Density functional theory

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APA (6^th Edition):

Fenta, A. E. S. (2019). Locally probing adatoms on graphene using perturbed angular correlation spectroscopy . (Thesis). Universidade de Aveiro. Retrieved from http://hdl.handle.net/10773/28060

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fenta, Abel Eduardo Silva. “Locally probing adatoms on graphene using perturbed angular correlation spectroscopy .” 2019. Thesis, Universidade de Aveiro. Accessed January 16, 2021. http://hdl.handle.net/10773/28060.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fenta, Abel Eduardo Silva. “Locally probing adatoms on graphene using perturbed angular correlation spectroscopy .” 2019. Web. 16 Jan 2021.

Vancouver:

Fenta AES. Locally probing adatoms on graphene using perturbed angular correlation spectroscopy . [Internet] [Thesis]. Universidade de Aveiro; 2019. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10773/28060.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fenta AES. Locally probing adatoms on graphene using perturbed angular correlation spectroscopy . [Thesis]. Universidade de Aveiro; 2019. Available from: http://hdl.handle.net/10773/28060

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Miami University

20. Zanfardino, Sara Marie. SENSITIVITY OF DIFFUSE CORRELATION SPECTROSCOPY TO FLOW RATES IN TISSUE-SIMULATING OPTICAL PHANTOMS.

Degree: MS, Physics, 2018, Miami University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=miami1533076561913823

► This thesis explored diffuse correlation spectroscopy (DCS) as a flow-monitoring technique along with its theoretical formalism and experimental applications. Experimental data as presented here has… (more)

Subjects/Keywords: Physics; diffuse correlation spectroscopy, tissue-simulating phantoms, diffuse optics

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APA (6^th Edition):

Zanfardino, S. M. (2018). SENSITIVITY OF DIFFUSE CORRELATION SPECTROSCOPY TO FLOW RATES IN TISSUE-SIMULATING OPTICAL PHANTOMS. (Masters Thesis). Miami University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=miami1533076561913823

Chicago Manual of Style (16^th Edition):

Zanfardino, Sara Marie. “SENSITIVITY OF DIFFUSE CORRELATION SPECTROSCOPY TO FLOW RATES IN TISSUE-SIMULATING OPTICAL PHANTOMS.” 2018. Masters Thesis, Miami University. Accessed January 16, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=miami1533076561913823.

MLA Handbook (7^th Edition):

Zanfardino, Sara Marie. “SENSITIVITY OF DIFFUSE CORRELATION SPECTROSCOPY TO FLOW RATES IN TISSUE-SIMULATING OPTICAL PHANTOMS.” 2018. Web. 16 Jan 2021.

Vancouver:

Zanfardino SM. SENSITIVITY OF DIFFUSE CORRELATION SPECTROSCOPY TO FLOW RATES IN TISSUE-SIMULATING OPTICAL PHANTOMS. [Internet] [Masters thesis]. Miami University; 2018. [cited 2021 Jan 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1533076561913823.

Council of Science Editors:

Zanfardino SM. SENSITIVITY OF DIFFUSE CORRELATION SPECTROSCOPY TO FLOW RATES IN TISSUE-SIMULATING OPTICAL PHANTOMS. [Masters Thesis]. Miami University; 2018. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1533076561913823

University of Kentucky

21. Zhao, Mingjun. NONINVASIVE MULTIMODAL DIFFUSE OPTICAL IMAGING OF VULNERABLE TISSUE HEMODYNAMICS.

Degree: 2019, University of Kentucky

URL: https://uknowledge.uky.edu/cbme_etds/58

► Measurement of tissue hemodynamics provides vital information for the assessment of tissue viability. This thesis reports three noninvasive near-infrared diffuse optical systems for spectroscopic measurements… (more)

▼ Measurement of tissue hemodynamics provides vital information for the assessment of tissue viability. This thesis reports three noninvasive near-infrared diffuse optical systems for spectroscopic measurements and tomographic imaging of tissue hemodynamics in vulnerable tissues with the goal of disease diagnosis and treatment monitoring. A hybrid near-infrared spectroscopy/diffuse correlation spectroscopy (NIRS/DCS) instrument with a contact fiber-optic probe was developed and utilized for simultaneous and continuous monitoring of blood flow (BF), blood oxygenation, and oxidative metabolism in exercising gastrocnemius. Results measured by the hybrid NIRS/DCS instrument in 37 subjects (mean age: 67 ± 6) indicated that vitamin D supplement plus aerobic training improved muscle metabolic function in older population. To reduce the interference and potential infection risk on vulnerable tissues caused by the contact measurement, a noncontact diffuse correlation spectroscopy/tomography (ncDCS/ncDCT) system was then developed. The ncDCS/ncDCT system employed optical lenses to project limited numbers of sources and detectors on the tissue surface. A motor-driven noncontact probe scanned over a region of interest to collect boundary data for three dimensional (3D) tomographic imaging of blood flow distribution. The ncDCS was tested for BF measurements in mastectomy skin flaps. Nineteen (19) patients underwent mastectomy and implant-based breast reconstruction were measured before and immediately after mastectomy. The BF index after mastectomy in each patient was normalized to its baseline value before surgery to get relative BF (rBF). Since rBF values in the patients with necrosis (n = 4) were significantly lower than those without necrosis (n = 15), rBF levels can be used to predict mastectomy skin flap necrosis. The ncDCT was tested for 3D imaging of BF distributions in chronic wounds of 5 patients. Spatial variations in BF contrasts over the wounded tissues were observed, indicating the capability of ncDCT in detecting tissue hemodynamic heterogeneities. To improve temporal/spatial resolution and avoid motion artifacts due to a long mechanical scanning of ncDCT, an electron-multiplying charge-coupled device based noncontact speckle contrast diffuse correlation tomography (scDCT) was developed. Validation of scDCT was done by imaging both high and low BF contrasts in tissue-like phantoms and human forearms. In a wound imaging study using scDCT, significant lower BF values were observed in the burned areas/volumes compared to surrounding normal tissues in two patients with burn. One limitation in this study was the potential influence of other unknown tissue optical properties such as tissue absorption coefficient (µa) on BF measurements. A new algorithm was then developed to extract both µa and BF using light intensities and speckle contrasts measured by scDCT at multiple source-detector distances. The new algorithm was validated using tissue-like liquid phantoms with varied values of µa and BF index. In-vivo validation and…

Subjects/Keywords: Hybrid Near Infrared Spectroscopy/ Diffuse Correlation Spectroscopy (hybrid NIRS/DCS); Speckle Contrast Diffuse Correlation Tomography (scDCT); Noncontact Diffuse Correlation Tomography (ncDCT); Muscle; Flap; Wound; Bioimaging and Biomedical Optics

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APA (6^th Edition):

Zhao, M. (2019). NONINVASIVE MULTIMODAL DIFFUSE OPTICAL IMAGING OF VULNERABLE TISSUE HEMODYNAMICS. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/cbme_etds/58

Chicago Manual of Style (16^th Edition):

Zhao, Mingjun. “NONINVASIVE MULTIMODAL DIFFUSE OPTICAL IMAGING OF VULNERABLE TISSUE HEMODYNAMICS.” 2019. Doctoral Dissertation, University of Kentucky. Accessed January 16, 2021. https://uknowledge.uky.edu/cbme_etds/58.

MLA Handbook (7^th Edition):

Zhao, Mingjun. “NONINVASIVE MULTIMODAL DIFFUSE OPTICAL IMAGING OF VULNERABLE TISSUE HEMODYNAMICS.” 2019. Web. 16 Jan 2021.

Vancouver:

Zhao M. NONINVASIVE MULTIMODAL DIFFUSE OPTICAL IMAGING OF VULNERABLE TISSUE HEMODYNAMICS. [Internet] [Doctoral dissertation]. University of Kentucky; 2019. [cited 2021 Jan 16]. Available from: https://uknowledge.uky.edu/cbme_etds/58.

Council of Science Editors:

Zhao M. NONINVASIVE MULTIMODAL DIFFUSE OPTICAL IMAGING OF VULNERABLE TISSUE HEMODYNAMICS. [Doctoral Dissertation]. University of Kentucky; 2019. Available from: https://uknowledge.uky.edu/cbme_etds/58

RMIT University

22. Ing, D. On the application of theoretical multi-dimensional coherence spectroscopy techniques to discrete quantum systems.

Degree: 2018, RMIT University

URL: http://researchbank.rmit.edu.au/view/rmit:162395

► Multi-dimensional coherence spectroscopy (MDCS) is a powerful technique that allows insight about the energy dynamics of quantum systems, on the timescale in which they occur.… (more)

Subjects/Keywords: Fields of Research; multi-dimensional coherence spectroscopy; coherence spectroscopy; two-dimensional electronic spectroscopy; 2DES; MDCS; nitrogen-vacancy; decoherence probe; spatial correlation

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APA (6^th Edition):

Ing, D. (2018). On the application of theoretical multi-dimensional coherence spectroscopy techniques to discrete quantum systems. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:162395

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ing, D. “On the application of theoretical multi-dimensional coherence spectroscopy techniques to discrete quantum systems.” 2018. Thesis, RMIT University. Accessed January 16, 2021. http://researchbank.rmit.edu.au/view/rmit:162395.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ing, D. “On the application of theoretical multi-dimensional coherence spectroscopy techniques to discrete quantum systems.” 2018. Web. 16 Jan 2021.

Vancouver:

Ing D. On the application of theoretical multi-dimensional coherence spectroscopy techniques to discrete quantum systems. [Internet] [Thesis]. RMIT University; 2018. [cited 2021 Jan 16]. Available from: http://researchbank.rmit.edu.au/view/rmit:162395.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ing D. On the application of theoretical multi-dimensional coherence spectroscopy techniques to discrete quantum systems. [Thesis]. RMIT University; 2018. Available from: http://researchbank.rmit.edu.au/view/rmit:162395

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

23. Yirdaw, Robel Birru. Conformational dynamics of proteins by fluorescence fluctuation spectroscopy.

Degree: PhD, Physics, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/12826

► Proteins, as intrinsically flexible molecules, exhibit internal motions at equilibrium. In general, the internal motions consist of changes in the three dimensional coordinates of the… (more)

▼ Proteins, as intrinsically flexible molecules, exhibit internal motions at equilibrium. In general, the internal motions consist of changes in the three dimensional coordinates of the constituent atoms. These motions are collectively referred to as conformational dynamics and span multiple orders of magnitude in timescale. Protein conformational dynamics is central to biological processes. In this work, fluorescence fluctuation spectroscopy (FFS), a single molecule technique, was utilized to study the conformational dynamics of Bacteriophage T4 lysozyme (T4L). Bacteriophage T4 is a virus that infects E. coli and T4L is used to break down the E. coli cell wall in the late stages of the infection cycle. The structure of T4L consists of two domains joined by a long helix with the active site situated in between the two domains. The presence of equilibrium conformational dynamics in T4L consisting of the motion of one domain relative to the other has long been postulated. In the work presented here, fluorescence correlation spectroscopy (FCS) and brightness (cumulant) analysis, two complementary variations of FFS, were used to investigate T4 lysozyme conformational dynamics. FCS results give direct evidence for conformational dynamics in T4L consisting of changes in relative distance and orientation of the two domains with a relaxation time of approximately 15 µs. The amplitude of this motion diminishes upon covalent substrate trapping. FCS results indicate the presence of dynamics along the long helix that persists upon substrate binding which may be necessary to facilitate the opening and closing of the active site. Furthermore, in contrast to a motion that involves discrete open and closed conformational states, results point to the presence of multiple conformations. Moreover, FFS was applied on Ca²⁺/calmodulin-dependent protein kinase II (CAMKII) and the membrane transporter MsbA. Relative to T4L, these two serve as examples of complex protein systems. The particular challenges in applying FFS to such systems are presented. The use of brightness analysis, a relatively new approach compared to FCS, for the study of protein conformational dynamics is discussed. As part of this work, data analysis and simulation software tools were developed using MATLAB. Advisors/Committee Members: David W. Piston (committee member), Thomas J. Weiler (committee member), Kalman Varga (committee member), M. Shane Hutson (committee member), Hassane S. Mchaourab (Committee Chair).

Subjects/Keywords: Single Molecule Fluorescence Spectroscopy; T4 Lysozyme; Fluorescence Fluctuation Spectroscopy; Conformational Dynamics; Protein; Fluorescence Correlation Spectroscopy; Brightness Analysis; Cumulant Analysis

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APA (6^th Edition):

Yirdaw, R. B. (2012). Conformational dynamics of proteins by fluorescence fluctuation spectroscopy. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12826

Chicago Manual of Style (16^th Edition):

Yirdaw, Robel Birru. “Conformational dynamics of proteins by fluorescence fluctuation spectroscopy.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed January 16, 2021. http://hdl.handle.net/1803/12826.

MLA Handbook (7^th Edition):

Yirdaw, Robel Birru. “Conformational dynamics of proteins by fluorescence fluctuation spectroscopy.” 2012. Web. 16 Jan 2021.

Vancouver:

Yirdaw RB. Conformational dynamics of proteins by fluorescence fluctuation spectroscopy. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1803/12826.

Council of Science Editors:

Yirdaw RB. Conformational dynamics of proteins by fluorescence fluctuation spectroscopy. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/12826

University of Pennsylvania

24. Lynch, Jennifer Marie. Investigations of Cerebral Hemodynamics in Infants with Critical Congenital Heart Disease Using Diffuse Optics.

Degree: 2014, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1353

► Congenital heart defects (CHD) are the most common type of birth defect, affecting approximately 30,000 children each year, one third of whom require cardiac surgery… (more)

▼ Congenital heart defects (CHD) are the most common type of birth defect, affecting approximately 30,000 children each year, one third of whom require cardiac surgery in their first year of life. Surgical advances have improved the cardiac outcomes for these children, and since the majority of these patients now reach school age, the research focus has shifted to address neurodevelopmental difficulties of survivors. A key physiological factor appears to be the high prevalence of hypoxic-ischemic white mater brain injury observed in these children. The exact timing of the injury occurrence, however, is difficult to ascertain due to limitations of the imaging modalities employed for this fragile, infant population. This thesis develops and explores the use of diffuse optical spectroscopy techniques for investigation of the risk factors for hypoxic-ischemic brain injury in these infants. The optical techniques utilize near-infrared (NIR) light and the diffusion approximation to model light transport in order to probe the static and dynamic properties of tissue. Frequency-domain diffuse optical spectroscopy (FD-DOS) is a technology, similar to widely used near-infrared spectroscopy (NIRS), that permits quantification of tissue oxygen saturation and total hemoglobin concentration. Diffuse correlation spectroscopy (DCS) is a relatively newer technique, centered on an idea similar to dynamic light scattering, which enables quantification of blood flow. Both FD-DOS and DCS are used in this research. The experiments presented in this thesis explore a variety of biophysics and biomedical questions. Arguably, the most important clinical findings to emerge from this dissertation are new risk factors associated with brain injury in infants with a certain form of CHD called hypoplastic left heart syndrome (HLHS). Using the aforementioned optical techniques, we found that longer time-to-surgery, lower cerebral oxygen saturation, and higher cerebral blood flow measured on the morning of surgery were associated with the risk of acquiring post-operative brain injury in this cohort. The results are novel for the community and shift our understanding of when these neonates are most at risk for acquiring brain injury. Most importantly, these results and the technology developed should improve current clinical care of this patient population.

Subjects/Keywords: biomedical optics; congenital heart disease; diffuse correlation spectroscopy; diffuse optical spectroscopy; near-infrared spectroscopy; periventricular leukomalacia; Physics

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APA (6^th Edition):

Lynch, J. M. (2014). Investigations of Cerebral Hemodynamics in Infants with Critical Congenital Heart Disease Using Diffuse Optics. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lynch, Jennifer Marie. “Investigations of Cerebral Hemodynamics in Infants with Critical Congenital Heart Disease Using Diffuse Optics.” 2014. Thesis, University of Pennsylvania. Accessed January 16, 2021. https://repository.upenn.edu/edissertations/1353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lynch, Jennifer Marie. “Investigations of Cerebral Hemodynamics in Infants with Critical Congenital Heart Disease Using Diffuse Optics.” 2014. Web. 16 Jan 2021.

Vancouver:

Lynch JM. Investigations of Cerebral Hemodynamics in Infants with Critical Congenital Heart Disease Using Diffuse Optics. [Internet] [Thesis]. University of Pennsylvania; 2014. [cited 2021 Jan 16]. Available from: https://repository.upenn.edu/edissertations/1353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lynch JM. Investigations of Cerebral Hemodynamics in Infants with Critical Congenital Heart Disease Using Diffuse Optics. [Thesis]. University of Pennsylvania; 2014. Available from: https://repository.upenn.edu/edissertations/1353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kentucky

25. Irwin, Daniel. INFLUENCE OF TISSUE ABSORPTION AND SCATTERING ON DIFFUSE CORRELATION SPECTROSCOPY BLOOD FLOW MEASUREMENTS.

Degree: 2011, University of Kentucky

URL: https://uknowledge.uky.edu/gradschool_theses/136

► This investigation evaluates the influences of optical property assumptions on nearinfrared diffuse correlation spectroscopy (DCS) flow index measurements. Independent variation is induced in optical properties,… (more)

Subjects/Keywords: Diffuse Correlation Spectroscopy; Diffusing Wave Spectroscopy; Near Infrared Spectroscopy; Tissue Optical Properties; Blood Flow; Biological Engineering

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APA (6^th Edition):

Irwin, D. (2011). INFLUENCE OF TISSUE ABSORPTION AND SCATTERING ON DIFFUSE CORRELATION SPECTROSCOPY BLOOD FLOW MEASUREMENTS. (Masters Thesis). University of Kentucky. Retrieved from https://uknowledge.uky.edu/gradschool_theses/136

Chicago Manual of Style (16^th Edition):

Irwin, Daniel. “INFLUENCE OF TISSUE ABSORPTION AND SCATTERING ON DIFFUSE CORRELATION SPECTROSCOPY BLOOD FLOW MEASUREMENTS.” 2011. Masters Thesis, University of Kentucky. Accessed January 16, 2021. https://uknowledge.uky.edu/gradschool_theses/136.

MLA Handbook (7^th Edition):

Irwin, Daniel. “INFLUENCE OF TISSUE ABSORPTION AND SCATTERING ON DIFFUSE CORRELATION SPECTROSCOPY BLOOD FLOW MEASUREMENTS.” 2011. Web. 16 Jan 2021.

Vancouver:

Irwin D. INFLUENCE OF TISSUE ABSORPTION AND SCATTERING ON DIFFUSE CORRELATION SPECTROSCOPY BLOOD FLOW MEASUREMENTS. [Internet] [Masters thesis]. University of Kentucky; 2011. [cited 2021 Jan 16]. Available from: https://uknowledge.uky.edu/gradschool_theses/136.

Council of Science Editors:

Irwin D. INFLUENCE OF TISSUE ABSORPTION AND SCATTERING ON DIFFUSE CORRELATION SPECTROSCOPY BLOOD FLOW MEASUREMENTS. [Masters Thesis]. University of Kentucky; 2011. Available from: https://uknowledge.uky.edu/gradschool_theses/136

Indian Institute of Science

26. Chandran, Sriram R. Ultrasound-Assisted Diffuse Correlation Spectroscopy : Recovery of Local Dynamics and Mechanical Properties in Soft Condensed Matter Materials.

Degree: PhD, faculty of Engineering, 2017, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2753

► This thesis describes the development and applications of an extension of DWS which enables the recovery of ‘localized’ mechanical properties, in a specified region of… (more)

▼ This thesis describes the development and applications of an extension of DWS which enables the recovery of ‘localized’ mechanical properties, in a specified region of a complex jelly-like object which is inhomogeneous, marked out by the focal volume of an ultrasound transducer, also called the region-of-interest (ROI). Introduction of the sinusoidal forcing creates a sinusoidal phase variation in the detected light in a DWS experiment which modulates the measured intensity autocorrelation, g2 (τ ). Decay in the modulation depth with τ is used to recover the visco-elastic spectrum of the material in the ROI. En route to this, growth of the mean-squared dis- placement (MSD) with time is extracted from the modulation depth decay, which was verified first by the usual DWS experimental data from an homogeneous object with properties matching those in the ROI of the inhomogeneous object and then those obtained by solving the generalized Langevin equation (GLE) modelling the dynamics of a typical scattering centre in the ROI. A region-specific visco-elastic spectral map was obtained by scanning the inhomogeneous object by the ultrasound focal volume. Further, the resonant modes of the vibrating ROI were measured by locating the peaks of the modulation depth variation in g2(τ ) with respect to the ultrasound frequency. These resonant modes were made use of to recover elasticity of the material of the object in the ROI. Using a similar strategy, it was also shown that flow in pipe can be detected and flow rate computed by ‘tagging’ the photons passing through the pipe with a focussed ultrasound beam. It is demonstrated, both through experiments and simulations that the ultrasound-assisted technique devel- oped is better suited to both detect and quantitatively assess flow in a background of Brownian dynamics than the usual DWS. In particular, the MSD of particles in the flow, which shows forth a super-diffusive dynamics with MSD growing following τ α with α < 2, is captured over larger intervals of τ than was possible using existing methods. On the theoretical front, the main contribution is the derivation of the GLE, with multiplicative noise modulating the interaction ‘spring constant’. The noise is derived as an average effect of the micropolar rotations suffered by the ‘bath’ particles on the ‘system’ particle modelled. It has been shown that the ‘local’ dynamics of the system particle is nontrivially influenced by the dynamics, both translation and rotation, of ‘nonlocal’ bath particles. Advisors/Committee Members: Vasu, R M (advisor).

Subjects/Keywords: Diffuse Correlation Spectroscopy; Soft Condensed Matter; Visco-Elastic Spectrum; Diffusing Wave Spectroscopy (DWS); Ultrasound Transducers; Condensed Matter; Ultrasound Diffuse Correlation Spectrosocpy; Elasticity; Viscoelasticity; Ultrasound Modulated Optical Tomography; Diffusing-wave Spectroscopy; Ultrasound-assisted Diffuse Correlation Spectroscopy; Applied Physics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chandran, S. R. (2017). Ultrasound-Assisted Diffuse Correlation Spectroscopy : Recovery of Local Dynamics and Mechanical Properties in Soft Condensed Matter Materials. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2753

Chicago Manual of Style (16^th Edition):

Chandran, Sriram R. “Ultrasound-Assisted Diffuse Correlation Spectroscopy : Recovery of Local Dynamics and Mechanical Properties in Soft Condensed Matter Materials.” 2017. Doctoral Dissertation, Indian Institute of Science. Accessed January 16, 2021. http://etd.iisc.ac.in/handle/2005/2753.

MLA Handbook (7^th Edition):

Chandran, Sriram R. “Ultrasound-Assisted Diffuse Correlation Spectroscopy : Recovery of Local Dynamics and Mechanical Properties in Soft Condensed Matter Materials.” 2017. Web. 16 Jan 2021.

Vancouver:

Chandran SR. Ultrasound-Assisted Diffuse Correlation Spectroscopy : Recovery of Local Dynamics and Mechanical Properties in Soft Condensed Matter Materials. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2017. [cited 2021 Jan 16]. Available from: http://etd.iisc.ac.in/handle/2005/2753.

Council of Science Editors:

Chandran SR. Ultrasound-Assisted Diffuse Correlation Spectroscopy : Recovery of Local Dynamics and Mechanical Properties in Soft Condensed Matter Materials. [Doctoral Dissertation]. Indian Institute of Science; 2017. Available from: http://etd.iisc.ac.in/handle/2005/2753

27. WANG XI. THE STUDY OF NODAL PROTEIN DYNAMICS AND INTERACTIONS IN LIVING ZEBRAFISH EMBRYOS BY FLUORESCENCE CORRELATION AND CROSS-CORRELATION SPECTROSCOPY.

Degree: 2014, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/107384

Subjects/Keywords: Fluorescence correlation spectroscopy; Fluorescence cross-correlation spectroscopy; Nodal; Molecular interactions

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

XI, W. (2014). THE STUDY OF NODAL PROTEIN DYNAMICS AND INTERACTIONS IN LIVING ZEBRAFISH EMBRYOS BY FLUORESCENCE CORRELATION AND CROSS-CORRELATION SPECTROSCOPY. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/107384

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

XI, WANG. “THE STUDY OF NODAL PROTEIN DYNAMICS AND INTERACTIONS IN LIVING ZEBRAFISH EMBRYOS BY FLUORESCENCE CORRELATION AND CROSS-CORRELATION SPECTROSCOPY.” 2014. Thesis, National University of Singapore. Accessed January 16, 2021. http://scholarbank.nus.edu.sg/handle/10635/107384.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

XI, WANG. “THE STUDY OF NODAL PROTEIN DYNAMICS AND INTERACTIONS IN LIVING ZEBRAFISH EMBRYOS BY FLUORESCENCE CORRELATION AND CROSS-CORRELATION SPECTROSCOPY.” 2014. Web. 16 Jan 2021.

Vancouver:

XI W. THE STUDY OF NODAL PROTEIN DYNAMICS AND INTERACTIONS IN LIVING ZEBRAFISH EMBRYOS BY FLUORESCENCE CORRELATION AND CROSS-CORRELATION SPECTROSCOPY. [Internet] [Thesis]. National University of Singapore; 2014. [cited 2021 Jan 16]. Available from: http://scholarbank.nus.edu.sg/handle/10635/107384.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

XI W. THE STUDY OF NODAL PROTEIN DYNAMICS AND INTERACTIONS IN LIVING ZEBRAFISH EMBRYOS BY FLUORESCENCE CORRELATION AND CROSS-CORRELATION SPECTROSCOPY. [Thesis]. National University of Singapore; 2014. Available from: http://scholarbank.nus.edu.sg/handle/10635/107384

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Delaware

28. Sobieski, Brian Joseph. Characterization of a bio-based, biodegradable class of copolymers, poly[(r)-3-hydroxybutyrate-co-(r)-3- hydroxyhexanoate], and application development.

Degree: PhD, University of Delaware. ǂb Department of Materials Science and Engineering, 2017, University of Delaware

URL: http://udspace.udel.edu/handle/19716/21738

► As modern society begins to focus on sustainability and renewable resources there is a growing need for the polymer industry to develop more environmentally friendly… (more)

▼ As modern society begins to focus on sustainability and renewable resources there is a growing need for the polymer industry to develop more environmentally friendly materials and practices. Part of this movement can be seen in the use of recycled materials in new products and in the development of bio-based, biodegradable polymers. Bio-based, biodegradable polymers are produced from renewable carbon sources, such as vegetable oils, typically polymerized using fermentation reactions via bacteria, and are able to be consumed by bacteria in landfills to completely convert the polymers to water and CO2. One class of such polymers are poly(hydroxyalkanoate)'s (PHAs), which are chiral, aliphatic polyesters. Within this class of polyesters are poly(hydroxybutyrate) (PHB) and the copolymer poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] (PHBHx), which have received extensive study due to their material properties as thermoplastics. Although the properties of PHB have been widely explored, much still remains to be understood about these promising biodegradable polymers. Specifically, PHB and its copolymers exhibit physical gelation in most solvents, yet the origin and mechanism of gelation and the properties of the resulting gel state are unknown. ☐ This research effort was primarily focused on investigating the physical gel state of PHBHx. Five goals were laid out and completed: determining the origin of gelation, the mechanism of gelation, the structure of the gel state, the properties of the gel state, and the effects of gelation on electrospun fibers of PHBHx. These goals were achieved through material characterization of the gel state utilizing infrared spectroscopy/two-dimensional correlation spectroscopy, differential scanning calorimetry, X-ray diffraction, scanning electron microscopy, and many other analysis methods. Crystallization of the polymer in solution was found to cause gelation in PHBHx solutions, where the polymer crystals act as tie points forming an interconnected network. The process of crystallization in solution was determined to follow the same method as crystallization in the bulk, neat polymer as it is cooled from a molten state. Morphological studies revealed that the polymer forms sub-micron fibrils and ribbons in the gel system forming an interconnected polymer network. The utility of this morphology combined with the bio-compatibility of PHBHx were demonstrated through growth of stem cells on the gel samples. Surprisingly, the stem cells did not differentiate and thrived on the freeze-dried PHBHx gels. These results indicate that the gel state of PHBHx could be used as a tissue engineering scaffold whose material properties can be tuned to the desired application without the concern of the stem cells differentiating into an unwanted cell type. Combined with the ease of generation of the PHBHx gels, these results show promising potential for industrial production of excellent three-dimensional culturing scaffolds. It was also found that the gels do not show signs of aging after gelation… Advisors/Committee Members: Rabolt, John F.Chase, D. BruceNoda, Isao.

Subjects/Keywords: Applied sciences; Infrared spectroscopy; P(hb-co-hhx) copolymers; Polymer characterization; Polymer gels; Thermal analysis; Two-dimensional (2D) correlation spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sobieski, B. J. (2017). Characterization of a bio-based, biodegradable class of copolymers, poly[(r)-3-hydroxybutyrate-co-(r)-3- hydroxyhexanoate], and application development. (Doctoral Dissertation). University of Delaware. Retrieved from http://udspace.udel.edu/handle/19716/21738

Chicago Manual of Style (16^th Edition):

Sobieski, Brian Joseph. “Characterization of a bio-based, biodegradable class of copolymers, poly[(r)-3-hydroxybutyrate-co-(r)-3- hydroxyhexanoate], and application development.” 2017. Doctoral Dissertation, University of Delaware. Accessed January 16, 2021. http://udspace.udel.edu/handle/19716/21738.

MLA Handbook (7^th Edition):

Sobieski, Brian Joseph. “Characterization of a bio-based, biodegradable class of copolymers, poly[(r)-3-hydroxybutyrate-co-(r)-3- hydroxyhexanoate], and application development.” 2017. Web. 16 Jan 2021.

Vancouver:

Sobieski BJ. Characterization of a bio-based, biodegradable class of copolymers, poly[(r)-3-hydroxybutyrate-co-(r)-3- hydroxyhexanoate], and application development. [Internet] [Doctoral dissertation]. University of Delaware; 2017. [cited 2021 Jan 16]. Available from: http://udspace.udel.edu/handle/19716/21738.

Council of Science Editors:

Sobieski BJ. Characterization of a bio-based, biodegradable class of copolymers, poly[(r)-3-hydroxybutyrate-co-(r)-3- hydroxyhexanoate], and application development. [Doctoral Dissertation]. University of Delaware; 2017. Available from: http://udspace.udel.edu/handle/19716/21738

Boston University

29. Sutin, Jason. Development of a novel diffuse correlation spectroscopy platform for monitoring cerebral blood flow and oxygen metabolism: from novel concepts and devices to preclinical live animal studies.

Degree: PhD, Pathology, 2017, Boston University

URL: http://hdl.handle.net/2144/20823

► New optical technologies were developed to continuously measure cerebral blood flow (CBF) and oxygen metabolism (CMRO2) non-invasively through the skull. Methods and devices were created… (more)

▼ New optical technologies were developed to continuously measure cerebral blood flow (CBF) and oxygen metabolism (CMRO2) non-invasively through the skull. Methods and devices were created to improve the performance of near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS) for use in experimental animals and humans. These were employed to investigate cerebral metabolism and cerebrovascular reactivity under different states of anesthesia and during models of pathological states. Burst suppression is a brain state arising naturally in pathological conditions or under deep general anesthesia, but its mechanism and consequences are not well understood. Electroencephalography (EEG) and cortical hemodynamics were simultaneously measured in rats to evaluate the coupling between cerebral oxygen metabolism and neuronal activity in the burst suppressed state. EEG bursts were used to deconvolve NIRS and DCS signals into the hemodynamic and metabolic response function for an individual burst. This response was found to be similar to the stereotypical functional hyperemia evoked by normal brain activation. Thus, spontaneous burst activity does not cause metabolic or hemodynamic dysfunction in the cortex. Furthermore, cortical metabolic activity was not associated with the initiation or termination of a burst. A novel technique, time-domain DCS (TD-DCS), was introduced to significantly increase the sensitivity of transcranial CBF measurements to the brain. A new time-correlated single photon counting (TCSPC) instrument with a custom high coherence pulsed laser source was engineered for the first-ever simultaneous measurement of photon time of flight and DCS autocorrelation decays. In this new approach, photon time tags are exploited to determine path-length-dependent autocorrelation functions. By correlating photons according to time of flight, CBF is distinguished from superficial blood flow. Experiments in phantoms and animals demonstrate TD-DCS has significantly greater sensitivity to the brain than existing transcranial techniques. Intracranial pressure (ICP) modulates both steady-state and pulsatile CBF, making CBF a potential marker for ICP. In particular, the critical closing pressure (CrCP) has been proposed as a surrogate measure of ICP. A new DCS device was developed to measure pulsatile CBF non-invasively. A novel method for estimating CrCP and ICP from DCS measurement of pulsatile microvascular blood flow in the cerebral cortex was demonstrated in rats.

Subjects/Keywords: Pathology; Burst suppression; Cerebral blood flow; Cerebral metabolic rate of oxygen; Critical closing pressure; Diffuse correlation spectroscopy; Near-infrared spectroscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sutin, J. (2017). Development of a novel diffuse correlation spectroscopy platform for monitoring cerebral blood flow and oxygen metabolism: from novel concepts and devices to preclinical live animal studies. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/20823

Chicago Manual of Style (16^th Edition):

Sutin, Jason. “Development of a novel diffuse correlation spectroscopy platform for monitoring cerebral blood flow and oxygen metabolism: from novel concepts and devices to preclinical live animal studies.” 2017. Doctoral Dissertation, Boston University. Accessed January 16, 2021. http://hdl.handle.net/2144/20823.

MLA Handbook (7^th Edition):

Sutin, Jason. “Development of a novel diffuse correlation spectroscopy platform for monitoring cerebral blood flow and oxygen metabolism: from novel concepts and devices to preclinical live animal studies.” 2017. Web. 16 Jan 2021.

Vancouver:

Sutin J. Development of a novel diffuse correlation spectroscopy platform for monitoring cerebral blood flow and oxygen metabolism: from novel concepts and devices to preclinical live animal studies. [Internet] [Doctoral dissertation]. Boston University; 2017. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2144/20823.

Council of Science Editors:

Sutin J. Development of a novel diffuse correlation spectroscopy platform for monitoring cerebral blood flow and oxygen metabolism: from novel concepts and devices to preclinical live animal studies. [Doctoral Dissertation]. Boston University; 2017. Available from: http://hdl.handle.net/2144/20823

University of Western Ontario

30. Verdecchia, Kyle J. Digging Deeper with Diffuse Correlation Spectroscopy.

Degree: 2016, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/3930

► Patients with neurological diseases are vulnerable to cerebral ischemia, which can lead to brain injury. In the intensive care unit (ICU), neuromonitoring techniques that can… (more)

▼ Patients with neurological diseases are vulnerable to cerebral ischemia, which can lead to brain injury. In the intensive care unit (ICU), neuromonitoring techniques that can detect flow reductions would enable timely administration of therapies aimed at restoring adequate cerebral perfusion, thereby avoiding damage to the brain. However, suitable bedside neuromonitoring methods sensitive to changes of blood flow and/or oxygen metabolism have yet to be established. Near-infrared spectroscopy (NIRS) is a promising technique capable of non-invasively monitoring flow and oxygenation. Specifically, diffuse correlation spectroscopy (DCS) and time-resolved (TR) NIRS can be used to monitor blood flow and tissue oxygenation, respectively, and combined to measuring oxidative metabolism. The work presented in this thesis focused on advancing a DCS/TR-NIRS hybrid system for acquiring these physiological measurements at the bedside. The application of NIRS for neuromonitoring is favourable in the neonatal ICU since the relatively thin scalp and skull of infants has minimal effect on the detected optical signal. Considering this application, the validation of a combined DCS/NIRS method for measuring the cerebral metabolic rate of oxygen (CMRO2) was investigated in Chapter 2. Although perfusion changes measured by DCS have been confirmed by various flow modalities, characterization of photon scattering in the brain is not clearly understood. Chapter 3 presents the first DCS study conducted directly on exposed cortex to confirm that the Brownian motion model is the best flow model for characterizing the DCS signal. Furthermore, a primary limitation of DCS is signal contamination from extracerebral tissues in the adult head, causing CBF to be underestimated. In Chapter 4, a multi-layered model was implemented to separate signal contributions from scalp and brain; derived CBF changes were compared to computed tomography perfusion. Overall, this thesis advances DCS techniques by (i) quantifying cerebral oxygen metabolism, (ii) confirming the more appropriate flow model for analyzing DCS data and (iii) demonstrating the ability of DCS to measure CBF accurately despite the presence of a thick (1-cm) extracerebral layer. Ultimately, the work completed in this thesis should help with the development of a hybrid DCS/NIRS system suitable for monitoring cerebral hemodynamics and energy metabolism in critical-ill patients.

Subjects/Keywords: Near-Infrared Spectroscopy; Diffuse Correlation Spectroscopy; Delayed Cerebral Ischemia; Cerebral Blood Flow; Cerebral Oxidative Metabolism; Medical Biophysics; Optics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Verdecchia, K. J. (2016). Digging Deeper with Diffuse Correlation Spectroscopy. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/3930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Verdecchia, Kyle J. “Digging Deeper with Diffuse Correlation Spectroscopy.” 2016. Thesis, University of Western Ontario. Accessed January 16, 2021. https://ir.lib.uwo.ca/etd/3930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Verdecchia, Kyle J. “Digging Deeper with Diffuse Correlation Spectroscopy.” 2016. Web. 16 Jan 2021.

Vancouver:

Verdecchia KJ. Digging Deeper with Diffuse Correlation Spectroscopy. [Internet] [Thesis]. University of Western Ontario; 2016. [cited 2021 Jan 16]. Available from: https://ir.lib.uwo.ca/etd/3930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Verdecchia KJ. Digging Deeper with Diffuse Correlation Spectroscopy. [Thesis]. University of Western Ontario; 2016. Available from: https://ir.lib.uwo.ca/etd/3930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations