subject:(Corneal confocal microscopy)

University of Manchester

1. Ferdousi, Maryam. ASSESSMENT OF CORNEAL PATHOLOGY USING CORNEAL CONFOCAL MICROSCOPY IN PERIPHERAL NEUROPATHIES.

Degree: 2016, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:303675

► The validity of corneal confocal microscopy (CCM) in assessing peripheral neuropathy has been studied extensively in several studies with a large cohort of subjects with… (more)

Subjects/Keywords: Corneal Confocal Microscopy; Peripheral neuropathy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferdousi, M. (2016). ASSESSMENT OF CORNEAL PATHOLOGY USING CORNEAL CONFOCAL MICROSCOPY IN PERIPHERAL NEUROPATHIES. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:303675

Chicago Manual of Style (16^th Edition):

Ferdousi, Maryam. “ASSESSMENT OF CORNEAL PATHOLOGY USING CORNEAL CONFOCAL MICROSCOPY IN PERIPHERAL NEUROPATHIES.” 2016. Doctoral Dissertation, University of Manchester. Accessed February 27, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:303675.

MLA Handbook (7^th Edition):

Ferdousi, Maryam. “ASSESSMENT OF CORNEAL PATHOLOGY USING CORNEAL CONFOCAL MICROSCOPY IN PERIPHERAL NEUROPATHIES.” 2016. Web. 27 Feb 2021.

Vancouver:

Ferdousi M. ASSESSMENT OF CORNEAL PATHOLOGY USING CORNEAL CONFOCAL MICROSCOPY IN PERIPHERAL NEUROPATHIES. [Internet] [Doctoral dissertation]. University of Manchester; 2016. [cited 2021 Feb 27]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:303675.

Council of Science Editors:

Ferdousi M. ASSESSMENT OF CORNEAL PATHOLOGY USING CORNEAL CONFOCAL MICROSCOPY IN PERIPHERAL NEUROPATHIES. [Doctoral Dissertation]. University of Manchester; 2016. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:303675

University of Manchester

2. Petropoulos, Ioannis. Corneal nerve pathology in diabetes.

Degree: PhD, 2013, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/corneal-nerve-pathology-in-diabetes(9912e560-1032-45cd-8f0a-0477d6605a98).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588113

► The accurate detection and quantification of human diabetic somatic polyneuropathy (DSPN) are important to define at risk patients, anticipate deterioration, and assess new therapies. Current… (more)

Subjects/Keywords: 616.4; Diabetic Neuropathy; Corneal Confocal Microscopy; Diabetes

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Petropoulos, I. (2013). Corneal nerve pathology in diabetes. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/corneal-nerve-pathology-in-diabetes(9912e560-1032-45cd-8f0a-0477d6605a98).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588113

Chicago Manual of Style (16^th Edition):

Petropoulos, Ioannis. “Corneal nerve pathology in diabetes.” 2013. Doctoral Dissertation, University of Manchester. Accessed February 27, 2021. https://www.research.manchester.ac.uk/portal/en/theses/corneal-nerve-pathology-in-diabetes(9912e560-1032-45cd-8f0a-0477d6605a98).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588113.

MLA Handbook (7^th Edition):

Petropoulos, Ioannis. “Corneal nerve pathology in diabetes.” 2013. Web. 27 Feb 2021.

Vancouver:

Petropoulos I. Corneal nerve pathology in diabetes. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Feb 27]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/corneal-nerve-pathology-in-diabetes(9912e560-1032-45cd-8f0a-0477d6605a98).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588113.

Council of Science Editors:

Petropoulos I. Corneal nerve pathology in diabetes. [Doctoral Dissertation]. University of Manchester; 2013. Available from: https://www.research.manchester.ac.uk/portal/en/theses/corneal-nerve-pathology-in-diabetes(9912e560-1032-45cd-8f0a-0477d6605a98).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588113

3. Ferdousi, Maryam. Assessment of corneal pathology using corneal confocal microscopy in peripheral neuropathies.

Degree: PhD, 2017, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/assessment-of-corneal-pathology-using-corneal-confocal-microscopy-in-peripheral-neuropathies(53cb63fb-b645-4389-9fed-f9a028eaea90).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756833

► The validity of corneal confocal microscopy (CCM) in assessing peripheral neuropathy has been studied extensively in several studies with a large cohort of subjects with… (more)

Subjects/Keywords: 617.7; Peripheral neuropathy; Corneal Confocal Microscopy

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferdousi, M. (2017). Assessment of corneal pathology using corneal confocal microscopy in peripheral neuropathies. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/assessment-of-corneal-pathology-using-corneal-confocal-microscopy-in-peripheral-neuropathies(53cb63fb-b645-4389-9fed-f9a028eaea90).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756833

Chicago Manual of Style (16^th Edition):

Ferdousi, Maryam. “Assessment of corneal pathology using corneal confocal microscopy in peripheral neuropathies.” 2017. Doctoral Dissertation, University of Manchester. Accessed February 27, 2021. https://www.research.manchester.ac.uk/portal/en/theses/assessment-of-corneal-pathology-using-corneal-confocal-microscopy-in-peripheral-neuropathies(53cb63fb-b645-4389-9fed-f9a028eaea90).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756833.

MLA Handbook (7^th Edition):

Ferdousi, Maryam. “Assessment of corneal pathology using corneal confocal microscopy in peripheral neuropathies.” 2017. Web. 27 Feb 2021.

Vancouver:

Ferdousi M. Assessment of corneal pathology using corneal confocal microscopy in peripheral neuropathies. [Internet] [Doctoral dissertation]. University of Manchester; 2017. [cited 2021 Feb 27]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/assessment-of-corneal-pathology-using-corneal-confocal-microscopy-in-peripheral-neuropathies(53cb63fb-b645-4389-9fed-f9a028eaea90).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756833.

Council of Science Editors:

Ferdousi M. Assessment of corneal pathology using corneal confocal microscopy in peripheral neuropathies. [Doctoral Dissertation]. University of Manchester; 2017. Available from: https://www.research.manchester.ac.uk/portal/en/theses/assessment-of-corneal-pathology-using-corneal-confocal-microscopy-in-peripheral-neuropathies(53cb63fb-b645-4389-9fed-f9a028eaea90).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756833

Queensland University of Technology

4. Dehghani, Cirous. Longitudinal assessment of corneal subbasal nerve morphology as a potential measure of diabetic peripheral neuropathy.

Degree: 2015, Queensland University of Technology

URL: http://eprints.qut.edu.au/84833/

► This thesis represents a significant step forward in developing a validated measure for diabetic peripheral neuropathy – a debilitating and prevalent complication of diabetes. The… (more)

Subjects/Keywords: Corneal confocal microscopy; Diabetic peripheral neuropathy; Subbasal nerve morphology; Natural history; Corneal nerve quantification

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dehghani, C. (2015). Longitudinal assessment of corneal subbasal nerve morphology as a potential measure of diabetic peripheral neuropathy. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/84833/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dehghani, Cirous. “Longitudinal assessment of corneal subbasal nerve morphology as a potential measure of diabetic peripheral neuropathy.” 2015. Thesis, Queensland University of Technology. Accessed February 27, 2021. http://eprints.qut.edu.au/84833/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dehghani, Cirous. “Longitudinal assessment of corneal subbasal nerve morphology as a potential measure of diabetic peripheral neuropathy.” 2015. Web. 27 Feb 2021.

Vancouver:

Dehghani C. Longitudinal assessment of corneal subbasal nerve morphology as a potential measure of diabetic peripheral neuropathy. [Internet] [Thesis]. Queensland University of Technology; 2015. [cited 2021 Feb 27]. Available from: http://eprints.qut.edu.au/84833/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dehghani C. Longitudinal assessment of corneal subbasal nerve morphology as a potential measure of diabetic peripheral neuropathy. [Thesis]. Queensland University of Technology; 2015. Available from: http://eprints.qut.edu.au/84833/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

5. Pritchard, Nicola. Corneal nerve structure and function as markers of diabetic neuropathy.

Degree: 2012, Queensland University of Technology

URL: https://eprints.qut.edu.au/52722/

► Diabetic neuropathy is a significant clinical problem that currently has no effective therapy, and in advanced cases, leads to foot ulceration and lower limb amputation.… (more)

▼ Diabetic neuropathy is a significant clinical problem that currently has no effective therapy, and in advanced cases, leads to foot ulceration and lower limb amputation. The accurate detection, characterisation and quantification of this condition are important in order to define at-risk patients, anticipate deterioration, monitor progression and assess new therapies. This thesis evaluates novel corneal methods of assessing diabetic neuropathy. Over the past several years two new non-invasive corneal markers have emerged, and in cross-sectional studies have demonstrated their ability to stratify the severity of this disease. Corneal confocal microscopy (CCM) allows quantification of corneal nerve parameters and non-contact corneal aesthesiometry (NCCA), the presumed functional correlate of corneal structure, assesses the sensitivity of the cornea. Both these techniques are quick to perform, produce little or no discomfort for the patient, and with automatic analysis paradigms developed, are suitable for clinical settings. Each has advantages and disadvantages over established techniques for assessing diabetic neuropathy. New information is presented regarding measurement bias of CCM images, and a unique sampling paradigm and associated accuracy determination method of combinations is described. A novel high-speed corneal nerve mapping procedure has been developed and application of this procedure in individuals with neuropathy has revealed regions of sub-basal nerve plexus that dictate further evaluation, as they appear to show earlier signs of damage than the central region of the cornea that has to date been examined. The discriminative capacity of corneal sensitivity measured by NCCA is revealed to have reasonable potential as a marker of diabetic neuropathy. Application of these new corneal markers for longitudinal evaluation of diabetic neuropathy has the potential to reduce dependence on more invasive, costly, and time-consuming assessments, such as skin biopsy.

Subjects/Keywords: repeatability; corneal nerves; diabetes; neuropathy; non-contact corneal aesthesiometry; corneal sensitivity; neuropathy disability score; ophthalmic markers; corneal confocal microscopy; corneal markers; diabetic neuropathy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pritchard, N. (2012). Corneal nerve structure and function as markers of diabetic neuropathy. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/52722/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pritchard, Nicola. “Corneal nerve structure and function as markers of diabetic neuropathy.” 2012. Thesis, Queensland University of Technology. Accessed February 27, 2021. https://eprints.qut.edu.au/52722/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pritchard, Nicola. “Corneal nerve structure and function as markers of diabetic neuropathy.” 2012. Web. 27 Feb 2021.

Vancouver:

Pritchard N. Corneal nerve structure and function as markers of diabetic neuropathy. [Internet] [Thesis]. Queensland University of Technology; 2012. [cited 2021 Feb 27]. Available from: https://eprints.qut.edu.au/52722/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pritchard N. Corneal nerve structure and function as markers of diabetic neuropathy. [Thesis]. Queensland University of Technology; 2012. Available from: https://eprints.qut.edu.au/52722/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Bradford

6. Alzubaidi, Rania S. M. Fully automated computer system for diagnosis of corneal diseases : development of image processing technologies for the diagnosis of Acanthamoeba and Fusarium diseases in confocal microscopy images.

Degree: PhD, 2017, University of Bradford

URL: http://hdl.handle.net/10454/17142

► Confocal microscopy demonstrated its value in the diagnosis of Acanthamoeba and fungal keratitis which considered sight-threatening corneal diseases. However, it can be difficult to find… (more)

Subjects/Keywords: Image processing; Cornea; Digital diagnostic; Confocal microscopy; Infectious keratitis; Acanthamoeba; Fusarium; Corneal diseases

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alzubaidi, R. S. M. (2017). Fully automated computer system for diagnosis of corneal diseases : development of image processing technologies for the diagnosis of Acanthamoeba and Fusarium diseases in confocal microscopy images. (Doctoral Dissertation). University of Bradford. Retrieved from http://hdl.handle.net/10454/17142

Chicago Manual of Style (16^th Edition):

Alzubaidi, Rania S M. “Fully automated computer system for diagnosis of corneal diseases : development of image processing technologies for the diagnosis of Acanthamoeba and Fusarium diseases in confocal microscopy images.” 2017. Doctoral Dissertation, University of Bradford. Accessed February 27, 2021. http://hdl.handle.net/10454/17142.

MLA Handbook (7^th Edition):

Alzubaidi, Rania S M. “Fully automated computer system for diagnosis of corneal diseases : development of image processing technologies for the diagnosis of Acanthamoeba and Fusarium diseases in confocal microscopy images.” 2017. Web. 27 Feb 2021.

Vancouver:

Alzubaidi RSM. Fully automated computer system for diagnosis of corneal diseases : development of image processing technologies for the diagnosis of Acanthamoeba and Fusarium diseases in confocal microscopy images. [Internet] [Doctoral dissertation]. University of Bradford; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10454/17142.

Council of Science Editors:

Alzubaidi RSM. Fully automated computer system for diagnosis of corneal diseases : development of image processing technologies for the diagnosis of Acanthamoeba and Fusarium diseases in confocal microscopy images. [Doctoral Dissertation]. University of Bradford; 2017. Available from: http://hdl.handle.net/10454/17142

7. Hammadi, Shumoos T. H. Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images.

Degree: PhD, 2018, University of Bradford

URL: http://hdl.handle.net/10454/16924

► Diabetic Peripheral Neuropathy (DPN) is one of the most common types of diabetes that can affect the cornea. An accurate analysis of the corneal epithelium… (more)

Subjects/Keywords: Medical imaging; Diabetic peripheral neuropathy; Corneal confocal microscopy; Automatic nerve segmentation; Corneal sub-basal epithelium; Anisotropic diffusion filtering; Corneal endothelial cells; Automatic cell segmentation; Fast Fourier transform; Watershed transformation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hammadi, S. T. H. (2018). Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images. (Doctoral Dissertation). University of Bradford. Retrieved from http://hdl.handle.net/10454/16924

Chicago Manual of Style (16^th Edition):

Hammadi, Shumoos T H. “Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images.” 2018. Doctoral Dissertation, University of Bradford. Accessed February 27, 2021. http://hdl.handle.net/10454/16924.

MLA Handbook (7^th Edition):

Hammadi, Shumoos T H. “Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images.” 2018. Web. 27 Feb 2021.

Vancouver:

Hammadi STH. Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images. [Internet] [Doctoral dissertation]. University of Bradford; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10454/16924.

Council of Science Editors:

Hammadi STH. Novel medical imaging technologies for processing epithelium and endothelium layers in corneal confocal images : developing automated segmentation and quantification algorithms for processing sub-basal epithelium nerves and endothelial cells for early diagnosis of diabetic neuropathy in corneal confocal microscope images. [Doctoral Dissertation]. University of Bradford; 2018. Available from: http://hdl.handle.net/10454/16924

8. Petropoulos, Ioannis. Corneal Nerve Pathology in Diabetes.

Degree: 2013, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:194220

►

The accurate detection and quantification of human diabetic somatic polyneuropathy (DSPN) are important to define at risk patients, anticipate deterioration, and assess new therapies. Current… (more)

Subjects/Keywords: Diabetic Neuropathy; Corneal Confocal Microscopy; Diabetes

…265 8.4.7 In vivo corneal confocal microscopy… …282 9.2 Repeatability of in vivo corneal confocal microscopy to quantify corneal nerve… …corneal confocal microscopy (Chapter 4)… …quantitative morphology of nerve fibres in corneal confocal microscopy imaging (chapter 5)… …Validity of detection of diabetic somatic polyneuropathy by in vivo corneal confocal microscopy…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Petropoulos, I. (2013). Corneal Nerve Pathology in Diabetes. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:194220

Chicago Manual of Style (16^th Edition):

Petropoulos, Ioannis. “Corneal Nerve Pathology in Diabetes.” 2013. Doctoral Dissertation, University of Manchester. Accessed February 27, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:194220.

MLA Handbook (7^th Edition):

Petropoulos, Ioannis. “Corneal Nerve Pathology in Diabetes.” 2013. Web. 27 Feb 2021.

Vancouver:

Petropoulos I. Corneal Nerve Pathology in Diabetes. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Feb 27]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:194220.

Council of Science Editors:

Petropoulos I. Corneal Nerve Pathology in Diabetes. [Doctoral Dissertation]. University of Manchester; 2013. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:194220

University of New South Wales

9. Tummanapalli, Shyam Sunder. Evaluation of corneal nerves and tear neuropeptides in diabetic peripheral neuropathy.

Degree: Optometry & Vision Science, 2020, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/65060 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:63698/SOURCE02?view=true

► Background: Corneal nerve fibers express diffusible, trophic neuropeptides such as substance P and calcitonin gene-related peptide (CGRP) into tears in response to neurogenic inflammation. Impaired… (more)

▼ Background: Corneal nerve fibers express diffusible, trophic neuropeptides such as substance P and calcitonin gene-related peptide (CGRP) into tears in response to neurogenic inflammation. Impaired corneal nerve fibers have been proposed as early indicators of diabetic peripheral neuropathy (DPN). However, the changes that occur in the concentration of these neuropeptides and their relationship with peripheral neuropathy in the diabetic cohort has not been explored.Aim: To demonstrate the changes the concentrations of substance P and CGRP in tears as a result of corneal denervation in diabetes and their association with severity of DPN. Methods: The concentrations of substance P and CGRP in flush tears were measured by enzyme-linked immunosorbent assay. Corneal nerve fibers were assessed using corneal confocal microscopy. Motor nerve axonal excitability tests were conducted to assess axonal function.Results: Age was identified as a confounding factor and controlled in all subsequent studies. Corneal nerve fiber loss was associated with early markers of axonal dysfunction and severity of neuropathy in type 1 diabetes, suggesting that corneal nerve loss is a generalized neuropathic process. There was a significant reduction in the concentration of substance P in tears in people with type 1 diabetic neuropathy. The concentration of substance P in tears was associated with corneal nerve loss and with the severity of peripheral neuropathy in type 1 diabetes. In type 2 diabetes, there was no difference in neuropeptides between groups, regardless of neuropathic status. In both type 1 and type 2 diabetes, corneal nerve parameters were significantly decreased in DPN. Corneal confocal microscopy had a better diagnostic performance than the nerve excitability measures for detecting DPN in a cohort of participants with type 1 and type 2 diabetes. Tear film substance P concentration had a relatively good diagnostic efficiency in the assessment of DPN and may be used as a potential proxy marker for peripheral neuropathy in type 1 diabetes, but not in type 2 diabetes. The co-existence of renal dysfunction with diabetes does have an added detrimental effect on corneal small nerve fibers.Conclusion: The ocular surface can indeed be a useful means to detect peripheral neuropathic status in diabetes. The measurement of tear film substance P offers significant promise in the detection of DPN. Advisors/Committee Members: Markoulli, Maria, Optometry & Vision Science, Faculty of Science, UNSW, Willcox, Mark, Optometry & Vision Science, Faculty of Science, UNSW, Poynten, Ann, Department of Endocrinology, Prince of Wales Hospital, Sydney.

Subjects/Keywords: Tear neuropeptide; Diabetic peripheral neuropathy; Corneal confocal microscopy; Total neuropathy score; Type 2 diabetes; Tear film; Corneal nerves; Nerve excitability studies; Substance P; Calcitonin gene-related peptide; Chronic kidney disease; Flush tears; Inferior whorl; Fractal dimension; Type 1 diabetes

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tummanapalli, S. S. (2020). Evaluation of corneal nerves and tear neuropeptides in diabetic peripheral neuropathy. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/65060 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:63698/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Tummanapalli, Shyam Sunder. “Evaluation of corneal nerves and tear neuropeptides in diabetic peripheral neuropathy.” 2020. Doctoral Dissertation, University of New South Wales. Accessed February 27, 2021. http://handle.unsw.edu.au/1959.4/65060 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:63698/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Tummanapalli, Shyam Sunder. “Evaluation of corneal nerves and tear neuropeptides in diabetic peripheral neuropathy.” 2020. Web. 27 Feb 2021.

Vancouver:

Tummanapalli SS. Evaluation of corneal nerves and tear neuropeptides in diabetic peripheral neuropathy. [Internet] [Doctoral dissertation]. University of New South Wales; 2020. [cited 2021 Feb 27]. Available from: http://handle.unsw.edu.au/1959.4/65060 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:63698/SOURCE02?view=true.

Council of Science Editors:

Tummanapalli SS. Evaluation of corneal nerves and tear neuropeptides in diabetic peripheral neuropathy. [Doctoral Dissertation]. University of New South Wales; 2020. Available from: http://handle.unsw.edu.au/1959.4/65060 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:63698/SOURCE02?view=true

10. Ambrósio Júnior, Renato. "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial".

Degree: PhD, Oftalmologia, 2004, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;

►

Objetivo: Verificar resposta após debridamento epitelial de córneas humanas. Métodos: Córneas normais foram submetidas a debridamento antes da cirurgia de enucleação. Realizou-se histologia, TUNEL, Ki67,… (more)

Subjects/Keywords: APOPTOSE; APOPTOSIS; CORNEAL STROMA/anatomy & histology; CORNEAL STROMA/cytology; CORNEAL STROMA/physiology; EPITÉLIO DA CÓRNEA/anatomia & histologia; EPITÉLIO DA CÓRNEA/cirurgia; EPITÉLIO DA CÓRNEA/crescimento & desenvolvimento; EPITHELIUM CORNEAL/anatomy & histology; EPITHELIUM CORNEAL/growth & development; EPITHELIUM CORNEAL/surgery; ESTROMA CORNEAL/anatomia & histologia; ESTROMA CORNEAL/citologia; ESTROMA CORNEAL/fisiopatologia; IMMUNOHISTOCHEMISTRY/methods; IMUNOHISTOQUÍMICA/métodos; MICROSCOPIA CONFOCAL/métodos; MICROSCOPIA DE FLUORESCÊNCIA/métodos; MICROSCOPIA ELETRÔNICA/métodos; MICROSCOPY CONFOCAL/methods; MICROSCOPY ELECTRON/methods; MICROSCOPY FLUORESCENCE/methods; MITOSE/fisiologia; MITOSIS/physiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ambrósio Júnior, R. (2004). "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial". (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;

Chicago Manual of Style (16^th Edition):

Ambrósio Júnior, Renato. “"Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial".” 2004. Doctoral Dissertation, University of São Paulo. Accessed February 27, 2021. http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;.

MLA Handbook (7^th Edition):

Ambrósio Júnior, Renato. “"Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial".” 2004. Web. 27 Feb 2021.

Vancouver:

Ambrósio Júnior R. "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial". [Internet] [Doctoral dissertation]. University of São Paulo; 2004. [cited 2021 Feb 27]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;.

Council of Science Editors:

Ambrósio Júnior R. "Estudo laboratorial da cicatrização de córneas humanas após debridamento epitelial". [Doctoral Dissertation]. University of São Paulo; 2004. Available from: http://www.teses.usp.br/teses/disponiveis/5/5149/tde-21102005-085247/ ;

11. Xu, Manlong. Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro.

Degree: 2015, University of Waterloo

URL: http://hdl.handle.net/10012/9426

► The purpose of this work was to develop a sensitive in vitro method for ocular toxicity testing; and to use this method to assess the… (more)

▼ The purpose of this work was to develop a sensitive in vitro method for ocular toxicity testing; and to use this method to assess the individual and combined effects of ophthalmic solutions, benzalkonium chloride (BAK), and ultraviolet (UV) radiation on the eye; as well as to investigate the detoxification of BAK in vitro. Using an in vitro test battery with immortalized human corneal epithelial cells (HCEC) and fluorescent dyes, the toxicity of differently preserved ophthalmic solutions was assessed in the first chapter of the thesis. The BAK-preserved solutions demonstrated the greatest adverse effect on HCEC, followed by the solutions with other preservatives. The preservative-free ophthalmic solution had the least adverse effect. The in vitro test battery demonstrated high sensitivity and good correlation with in vivo and clinical studies. PrestoBlue is a new resazurin-based reagent for assessing cell metabolic activity and cytotoxicity. The second chapter compared PrestoBlue, alamarBlue, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazonium bromide (MTT) in assessing cell viability of HCEC. The result showed that PrestoBlue was more sensitive than MTT, but similar to alamarBlue. The plate color, reading mode and plate storage up to 7 days did not affect the performance of the PrestoBlue assay. Using the in vitro test battery with the new reagent PrestoBlue, the third chapter assessed the individual and combined toxicity of UV radiation (UV A and UVB) and BAK on HCEC. The combined effects of UV plus BAK were either greater than (synergistic) or equal to (additive) the sum of individual effects. The synergistic effects occurred between a low dose UV radiation (0.1719 J/cm²) and low concentrations (0.001%, 0.002%, 0.003% and 0.004%) of BAK. The last chapter investigated the detoxification of BAK by UVC radiation. The result showed that BAK toxicity on HCEC can be neutralized by an appropriate dose of UVC radiation. After complete neutralization, the toxicity of BAK was similar to phosphate buffered saline. While, the antimicrobial effect of BAK against Pseudomonas aeruginosa was reduced at the same time. The results of this work suggest that BAK-free, especially preservative-free ophthalmic solutions are safer alternatives to BAK-preserved ones. The combined toxic effects of BAK and solar radiation should be taken into consideration in the risk assessment of BAK-preserved ophthalmic solutions. UVC radiation can be used to neutralize BAK toxicity. This detoxification may be of great value in utilizing the antimicrobial efficacy of BAK while minimizing its potential hazards. The in vitro test battery with cultured HCEC used for this research may provide a sensitive and meaningful approach for evaluating both individual and combined ocular toxicity of UV radiation and chemicals.

Subjects/Keywords: in vitro; ophthalmic solution; benzalkonium chloride; ultraviolet radiation; human corneal epithelial cells; cell viability; alamarBlue; PrestoBlue; MTT; Toxicity; tight junction; confocal microscopy; fluorescent viability dyes; synergistic effect; additive effect; neutralization; ZO-1; antimicrobial efficacy

14 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xu, M. (2015). Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/9426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xu, Manlong. “Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro.” 2015. Thesis, University of Waterloo. Accessed February 27, 2021. http://hdl.handle.net/10012/9426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xu, Manlong. “Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro.” 2015. Web. 27 Feb 2021.

Vancouver:

Xu M. Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro. [Internet] [Thesis]. University of Waterloo; 2015. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10012/9426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xu M. Toxicity of Ophthalmic Solutions, Benzalkonium Chloride and UV Radiation on Human Corneal Epithelial Cells in vitro. [Thesis]. University of Waterloo; 2015. Available from: http://hdl.handle.net/10012/9426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Elbita, Abdulhakim Mehemed. Efficient processing of corneal confocal microscopy images : development of a computer system for the pre-processing, feature extraction, classification, enhancement and registration of a sequence of corneal images.

Degree: PhD, 2013, University of Bradford

URL: http://hdl.handle.net/10454/6463

► Corneal diseases are one of the major causes of visual impairment and blindness worldwide. Used for diagnoses, a laser confocal microscope provides a sequence of… (more)

Subjects/Keywords: 617.7; Confocal microscopy, corneal; Image processing; Image classification; Image enhancement; Image registration, texture analysis; Algorithms; Statistical features; Neural networks; Medical imaging.

5 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Elbita, A. M. (2013). Efficient processing of corneal confocal microscopy images : development of a computer system for the pre-processing, feature extraction, classification, enhancement and registration of a sequence of corneal images. (Doctoral Dissertation). University of Bradford. Retrieved from http://hdl.handle.net/10454/6463

Chicago Manual of Style (16^th Edition):

Elbita, Abdulhakim Mehemed. “Efficient processing of corneal confocal microscopy images : development of a computer system for the pre-processing, feature extraction, classification, enhancement and registration of a sequence of corneal images.” 2013. Doctoral Dissertation, University of Bradford. Accessed February 27, 2021. http://hdl.handle.net/10454/6463.

MLA Handbook (7^th Edition):

Elbita, Abdulhakim Mehemed. “Efficient processing of corneal confocal microscopy images : development of a computer system for the pre-processing, feature extraction, classification, enhancement and registration of a sequence of corneal images.” 2013. Web. 27 Feb 2021.

Vancouver:

Elbita AM. Efficient processing of corneal confocal microscopy images : development of a computer system for the pre-processing, feature extraction, classification, enhancement and registration of a sequence of corneal images. [Internet] [Doctoral dissertation]. University of Bradford; 2013. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10454/6463.

Council of Science Editors:

Elbita AM. Efficient processing of corneal confocal microscopy images : development of a computer system for the pre-processing, feature extraction, classification, enhancement and registration of a sequence of corneal images. [Doctoral Dissertation]. University of Bradford; 2013. Available from: http://hdl.handle.net/10454/6463

University of Waterloo

13. McCanna, David. Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products.

Degree: 2009, University of Waterloo

URL: http://hdl.handle.net/10012/4338

► The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing,… (more)

▼ The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty associated with dose selection for clinical trials. The first section of this thesis describes how in vitro methods can be used to improve the prediction of the toxicity of chemicals and ophthalmic products. The proper utilization of in vitro methods can accurately predict toxic threshold levels and reduce animal use in product development. Sections two, three and four describe the development of new sensitive in vitro methods for predicting ocular toxicity. Maintaining the barrier function of the cornea is critical for the prevention of the penetration of infections microorganisms and irritating chemicals into the eye. Chapter 2 describes the development of a method for assessing the effects of chemicals on tight junctions using a human corneal epithelial and canine kidney epithelial cell line. In Chapter 3 a method that uses a primary organ culture for assessing single instillation and multiple instillation toxic effects is described. The ScanTox system was shown to be an ideal system to monitor the toxic effects over time as multiple readings can be taken of treated bovine lenses using the nondestructive method of assessing for the lens optical quality. Confirmations of toxic effects were made with the utilization of the viability dye alamarBlue. Chapter 4 describes the development of sensitive in vitro assays for detecting ocular toxicity by measuring the effects of chemicals on the mitochondrial integrity of bovine cornea, bovine lens epithelium and corneal epithelial cells, using fluorescent dyes. The goal of this research was to develop an in vitro test battery that can be used to accurately predict the ocular toxicity of new chemicals and ophthalmic formulations. By comparing the toxicity seen in vivo animals and humans with the toxicity response in these new in vitro methods, it was demonstrated that these in vitro methods can be utilized in a tiered testing strategy in the development of new chemicals and ophthalmic formulations.

Subjects/Keywords: in vitro; Alternative Methods; alamarBlue; rhodamine; tight junctions; bovine lens; viability; toxicity; mitochondria; mitochondria integrity; benzalkonium chloride; sodium dodecyl sulphate; sodium lauryl sulfate; scanning electron microscopy; Draize; Draize maximal average scores; zonula occludens; cosmetic directive; PETA; humane society; animal league; people for the ethical treatment of animals; interagency coordinating committee on the validation of alternative methods; ICCVAM; ECVAM; JaCVAM; BCOP; bovine cornea; ocular irritants; ocular irritation; ocular toxicity; human corneal epithelial cells; vitro; confocal; confocal microscopy; metabolic activity; optical quality; reactive oxygen species; contact lens; contact lens care solutions; barrier function; microbial keratitis; mulitipurpose solutions; tight junction; cell damage; claudin; ZO-1; occludin; MDCK; Madin; Madin-Darby; canine kidney cells; cornea; human cornea; human corneal epithelium; epithelial cell line; human corneal epithelial cell line; sodium fluorescein; sodium fluorescein permeability; fluorescein permeability; ocular surface; cell monolayer; Araki-Sasaki; cell physiology; risk assessment; safety assessment; ScanTox; scanning laser; lens epithelium; corneal cells; in vitro model; ophthalmic formulations; multiple instillation; back vertex; animal testing; rabbit testing; alternatives to animal testing; cultured bovine lens; toxicity of chemicals; irritation; irritants; laser scanner; organ culture; delayed toxicity; toxins; hydrogen peroxide; cornea toxicity; cornea toxicity models; prediction of human toxicity; no observable adverse effect level; lowest observable adverse effect level; NOAEL; LOAEL; toxicity thresholds; safety factors; cornea; uncertainty factors; preservatives; disinfectants; ophthalmic products; preclinical; preclinical testing; epithelial barrier; drug penetration; clinical confocal microscopy; animal rights; rabbit cornea; human cornea; human clinical effects; toxic; animal rights activist; sensitive measures; toxic effect; toxicity threshold; agar overlay; agar diffusion; agar overlay method; agar diffusion method; cytochrome; cytochrome c; apoptosis; necrotic; apoptotic; necrosis; caspase; rhodamine 123; resazuran; resorufin; cell death; cell viability; metabolic dye; microsomal; microsomal enzymes; cytotoxicity; cytotoxic; cytotoxic effect; MTT; XTT; WST-1; plasma membrane; mitochondrial; mitochondrial morphology; ocular toxicity potential; ocular toxicity; human corneal epithelial; cell line; confocal analysis; corneal epithelium; cell fluorescence; alamarBlue assay; rhodamine dye; animal welfare; toxic injury; degraded mitochondria; epithelial monolayer; disinfectants; membrane integrity; eye toxicity; eye; viability dye; toxicity in humans; human toxicity; effects on the mitochondria; mitochondrial toxicity; in vitro battery; in vitro test battery; ophthalmic eye drop; direct contact; product safety; cytotoxicity potential; molecular; molecular biology; refine reduce replace; sensitivity and relevance; sensitivity; relevance; rabbit ocular irritation test; product development; cytotoxicity models; cytotoxicity alternative methods; replacements for animal testing; three r's; beagle; tiered testing; tiered testing strategy; replacements animal testing; mechanistic toxicity; cornea mitochondria; dose response; threshold

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McCanna, D. (2009). Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/4338

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCanna, David. “Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products.” 2009. Thesis, University of Waterloo. Accessed February 27, 2021. http://hdl.handle.net/10012/4338.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCanna, David. “Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products.” 2009. Web. 27 Feb 2021.

Vancouver:

McCanna D. Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products. [Internet] [Thesis]. University of Waterloo; 2009. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10012/4338.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCanna D. Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products. [Thesis]. University of Waterloo; 2009. Available from: http://hdl.handle.net/10012/4338

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations