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You searched for subject:(Compound exocytosis). Showing records 1 – 2 of 2 total matches.

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University of Lund

1. Karanauskaite, Jovita. Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA.

Degree: 2008, University of Lund

Several high resolution electrophysiological techniques such as measurements of cell membrane capacitance and amperometry are used to detect exocytotic events. A novel method was developed in this study based on the expression of P2X2 receptor channel in secreting cells. The presence of ATP sensitive channel on the cell membrane permitted the electrical detection of minute (amoles) quantities of ATP and thus created a possibility to measure ATP released from a single insulin granule. The release of peptides and low molecular weight substances was explored by combining the detection of exocytotic events by P2X2 receptor method with imaging of secretory granules. It was found that in rat insulinoma cells these substances were released independently and with different time course: nucleotides - 280 ms and granular peptide marker IAPP - 2.2 s after the membrane depolarization. In 72% of all exocytotic events nucleotide release was not followed by the discharge of peptide cargo. The chief inhibitory neurotransmitter gamma-aminobutyric acid (GABA) is also found in pancreatic β-cells though its role in coordinating processes in the pancreas is unknown. Sub-cellular localization has not been fully resolved too. Electrical measurements of ATP, GABA and 5-HT release in rat pancreatic β-cells allowed us to demonstrate that at least 75% of GABA release events were attributable to the exocytosis of large dense core vesicles in these cells and its selective release was regulated by the size of the fusion pore. The combination of the cell capacitance measurements, the electrical measurements of ATP release and amperometric detection of 5-HT release demonstrated that in rat pancreatic β-cells ATP is stored and released by large dense core vesicles. The contribution of small synaptic like vesicles is below the detection levels of methods used. The possibility of multi-vesicular exocytosis in pancreatic β-cells was explored by the cell-attached capacitance measurements, the total internal reflection microscopy and by electrical ATP release measurements. It was found that global increase in [Ca2+]i promotes the formation of complexes of interconnected granules within cell cytoplasm that subsequently undergo exocytosis as one unit. This may lead to exocytosis of up to 15 granules simultaneously (compound exocytosis).

Subjects/Keywords: Clinical Medicine; ATP; LDCV; Insulin; exocytosis; Compound Exocytosis; Calcium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Karanauskaite, J. (2008). Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/1244545 ; https://portal.research.lu.se/ws/files/3217945/1244547.pdf

Chicago Manual of Style (16th Edition):

Karanauskaite, Jovita. “Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA.” 2008. Doctoral Dissertation, University of Lund. Accessed April 14, 2021. https://lup.lub.lu.se/record/1244545 ; https://portal.research.lu.se/ws/files/3217945/1244547.pdf.

MLA Handbook (7th Edition):

Karanauskaite, Jovita. “Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA.” 2008. Web. 14 Apr 2021.

Vancouver:

Karanauskaite J. Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA. [Internet] [Doctoral dissertation]. University of Lund; 2008. [cited 2021 Apr 14]. Available from: https://lup.lub.lu.se/record/1244545 ; https://portal.research.lu.se/ws/files/3217945/1244547.pdf.

Council of Science Editors:

Karanauskaite J. Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA. [Doctoral Dissertation]. University of Lund; 2008. Available from: https://lup.lub.lu.se/record/1244545 ; https://portal.research.lu.se/ws/files/3217945/1244547.pdf


University of Queensland

2. Do, Hoang Oanh. Structural and functional changes of pancreatic beta-cells during the progression of disease in the Leprdb model of type 2 diabetes.

Degree: School of Biomedical Sciences, 2016, University of Queensland

Subjects/Keywords: Type 2 diabetes; Pancreatic islets; Beta-cells; Insulin secretion; Two photon; db/db mice; Insulin granules dynamics; Progression of diabetes; Compound exocytosis; 0601 Biochemistry and Cell Biology; 0606 Physiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Do, H. O. (2016). Structural and functional changes of pancreatic beta-cells during the progression of disease in the Leprdb model of type 2 diabetes. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:386310

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Do, Hoang Oanh. “Structural and functional changes of pancreatic beta-cells during the progression of disease in the Leprdb model of type 2 diabetes.” 2016. Thesis, University of Queensland. Accessed April 14, 2021. http://espace.library.uq.edu.au/view/UQ:386310.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Do, Hoang Oanh. “Structural and functional changes of pancreatic beta-cells during the progression of disease in the Leprdb model of type 2 diabetes.” 2016. Web. 14 Apr 2021.

Vancouver:

Do HO. Structural and functional changes of pancreatic beta-cells during the progression of disease in the Leprdb model of type 2 diabetes. [Internet] [Thesis]. University of Queensland; 2016. [cited 2021 Apr 14]. Available from: http://espace.library.uq.edu.au/view/UQ:386310.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Do HO. Structural and functional changes of pancreatic beta-cells during the progression of disease in the Leprdb model of type 2 diabetes. [Thesis]. University of Queensland; 2016. Available from: http://espace.library.uq.edu.au/view/UQ:386310

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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