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1. Watanabe, Luís Fernando Maranho [UNESP]. Habilidade competitiva entre espécies invasivas de mosca-branca, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) em plantas de tomate e pimentão.

Degree: 2017, Universidade Estadual Paulista

A espécie de mosca-branca Bemisia tabaci é considerada uma das pragas mais importantes do mundo e está amplamente distribuída. Estudos recentes demonstraram que a B. tabaci não é composta por biótipos, mas sim por um complexo de espécies crípticas, morfologicamente idênticas. No Brasil, a espécie Middle East-Asia Minor1 (MEAM1, conhecida como biótipo B) é predominante nos cultivos desde a invasão nos anos 90. Em 2014, foi relatada pela primeira vez, no estado do Rio Grande do Sul, a presença da espécie Mediterranean (MED, conhecida como biótipo Q). Com base na importância da espécie MEAM1 no Brasil e da espécie MED em vários países da Europa e Ásia, o objetivo deste estudo foi avaliar a preferência das espécies e a oviposição em diferentes hospedeiros, bem como a habilidade competitiva entre as espécies invasivas e a predominância em plantas de tomate e pimentão. Para os ensaios de preferência por hospedeiros e oviposição foram utilizados plantas de algodão, feijão, pimentão e tomate. Os ensaios foram conduzidos com a contagem do número de insetos adultos em 12, 24 e 48 horas após o início e com a contagem dos ovos após 48 horas do início do ensaio. O número de adultos em algodão foi significativamente favorável à MED nos três períodos; para feijão, apenas em 12 horas favorável à MEAM1 e tomateiro não houve diferença. Para o número de ovos, em algodão e tomate, MED ovipositou mais comparada à MEAM1, enquanto que para feijão não houve diferença estatística. Os ensaios de habilidade competitiva foram conduzidos utilizando insetos recém-emergidos, onde 10 casais de insetos de cada uma das espécies crípticas foram colocados em seus hospedeiros para avaliação. Os resultados obtidos demonstraram que a espécie MED predominou sobre a espécie MEAM1 em pimentão híbrido Magali R na 4ª amostragem (120 dias após o início do experimento), enquanto que para o tomateiro cv Santa Clara, a espécie MEAM1 predominou sobre a MED também na 4ª amostragem (100 dias após o início do experimento), mostrando a preferência da espécie MED por pimentão e da espécie MEAM1 por tomateiro, indicando que problemas associados a vírus, bem como da mosca-branca como praga podem se agravar em pimentão.

The species of whitefly Bemisia tabaci is considered one of the most important pests in the world and is widely disseminated. Recent studies have shown that B. tabaci is not composed of biotypes, but rather by a complex of cryptic species, morphologically identical. In Brazil, the species Middle East-Asia Minor1 (MEAM1, known as biotype B) has been predominant in the fields since its invasion in the 1990s. In 2014, it was reported for the first time, in the state of Rio Grande do Sul, the presence of the Mediterranean species (MED, known as the Q biotype). The objective of this study was to evaluate the preference of these species and their oviposition in cotton, common bean, pepper and tomato, as well as the competitive displacement in tomato and pepper. The assays were conducted by counting the number of adult insects at 12, 24 and 48 hours after start…

Advisors/Committee Members: Krause-Sakate, Renate [UNESP], Moura, Mônika Fecury [UNESP], Universidade Estadual Paulista (UNESP).

Subjects/Keywords: Bemisia tabaci; Preferência hospedeiro; Habilidade competitiva; MEAM1; MED; Host preference; Competitive displacement

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Watanabe, L. F. M. [. (2017). Habilidade competitiva entre espécies invasivas de mosca-branca, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) em plantas de tomate e pimentão. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/150342

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Watanabe, Luís Fernando Maranho [UNESP]. “Habilidade competitiva entre espécies invasivas de mosca-branca, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) em plantas de tomate e pimentão.” 2017. Thesis, Universidade Estadual Paulista. Accessed September 24, 2019. http://hdl.handle.net/11449/150342.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Watanabe, Luís Fernando Maranho [UNESP]. “Habilidade competitiva entre espécies invasivas de mosca-branca, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) em plantas de tomate e pimentão.” 2017. Web. 24 Sep 2019.

Vancouver:

Watanabe LFM[. Habilidade competitiva entre espécies invasivas de mosca-branca, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) em plantas de tomate e pimentão. [Internet] [Thesis]. Universidade Estadual Paulista; 2017. [cited 2019 Sep 24]. Available from: http://hdl.handle.net/11449/150342.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Watanabe LFM[. Habilidade competitiva entre espécies invasivas de mosca-branca, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) em plantas de tomate e pimentão. [Thesis]. Universidade Estadual Paulista; 2017. Available from: http://hdl.handle.net/11449/150342

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Loughborough University

2. Sutar, Tina. New formats for affinity selection of human cells.

Degree: PhD, 2015, Loughborough University

Despite recent advances in stem cell biology, immunotherapy and transplantation, substantial barriers still exist in the large-scale specific separation of a discrete population of human therapeutic cells from a cell suspension. The ideal purification technique should combine high cell purity, yield and function, with fast processing and affordability. Currently, fluorescence-activated cell sorting with flow cytometry (FACS) and magnetic activated cell sorting (MACS®) are the most used methods for cell separation and purification and have been employed extensively in molecular biology, diagnostic and cell sorting applications, because they are considered to be gentle, fast and scalable. However, these methods have several key disadvantages; they are invariably expensive, yield low log cell reduction (LCR) rates, and suffer from drawbacks when applied to niche cell populations, such as those requiring multiple tandem separation steps and/or involving combined positive and negative cell selection steps. To address this challenge, a new cell affinity selection system was developed. The selectivity is based on the reversible monomeric avidin biotin interaction and it is primary designed for positive selection. The initial studies were performed on flat, nonporous, glass coverslips and the technology was then successfully transferred on high grade smooth non-porous glass beads (with a diameter of 79.12 to 118.59 μm). The multi-step layer-by-layer deposition procedure culminating in dextran-coated supports bearing monomeric avidin was rigorously characterized and subsequently employed in packed bed chromatography experiments with human erythrocytes isolated from cord blood and B lymphocytes from cell lines. The developed affinity selection platform was highly selective, efficient and, most importantly, resulted in high yields, cell purity and viability comparable with MACS® technology. Additionally scale up is possible and could be easily transferred to another chromatographic matrix with the appropriate structure.

Subjects/Keywords: 572; Affinity separation; Human therapeutic cells; Red blood cells; B lymphocytes; T lymphocytes; Dextran; Monomeric avidin; D- biotin; Competitive displacement; Glass coverslips; Non-porous glass beads; MACS®

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Sutar, T. (2015). New formats for affinity selection of human cells. (Doctoral Dissertation). Loughborough University. Retrieved from https://dspace.lboro.ac.uk/2134/17735 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658289

Chicago Manual of Style (16th Edition):

Sutar, Tina. “New formats for affinity selection of human cells.” 2015. Doctoral Dissertation, Loughborough University. Accessed September 24, 2019. https://dspace.lboro.ac.uk/2134/17735 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658289.

MLA Handbook (7th Edition):

Sutar, Tina. “New formats for affinity selection of human cells.” 2015. Web. 24 Sep 2019.

Vancouver:

Sutar T. New formats for affinity selection of human cells. [Internet] [Doctoral dissertation]. Loughborough University; 2015. [cited 2019 Sep 24]. Available from: https://dspace.lboro.ac.uk/2134/17735 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658289.

Council of Science Editors:

Sutar T. New formats for affinity selection of human cells. [Doctoral Dissertation]. Loughborough University; 2015. Available from: https://dspace.lboro.ac.uk/2134/17735 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658289

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