subject:(Compartmentalization)

University of Tennessee – Knoxville

1. Aggarwal, Ravi. Supported Aqueous-Phase Catalysis for Atom Transfer Radical Polymerization.

Degree: 2010, University of Tennessee – Knoxville

URL: http://trace.tennessee.edu/utk_graddiss/771

► Atom transfer radical polymerization (ATRP) which utilizes transition metal based catalysts is a versatile methodology for the synthesis of a wide spectrum of polymers with… (more)

▼ Atom transfer radical polymerization (ATRP) which utilizes transition metal based catalysts is a versatile methodology for the synthesis of a wide spectrum of polymers with controlled architectures. However, high concentrations of soluble catalyst required in an ATRP process makes the final polymer colored and toxic. Thus, the catalyst removal/reduction/recycling remains a challenge in the field of ATRP. Supported catalysts on insoluble solids such as silica gel, polystyrene beads, etc. have been used in ATRP to facilitate the catalyst recovery and recycling. However, the ability of the supported catalysts to mediate a polymerization is substantially reduced due to their reduced mobility and leaching problems. In this thesis, we report a series of novel and recyclable physisorbed CuBr2/N, N, N’, N’’-pentamethyldiethylene-triamine supported catalytic systems operating in conjunction with hydration. Supported aqueous-phase catalysis (SAPC) for ATRP was evaluated for different inorganic (Na-clay, silica and zeolite) and organic (polysaccharides) supports. The hydrated physisorbed supported catalysts were used for the polymerization of benzyl methacrylate and methyl methacrylate using an activator generated electron transfer ATRP process. The catalyst was effectively retained on the surface of supports through hydration as was verified by UV-Vis measurements. The supported catalyst was easily removed from the polymerization by simple filtration process affording a colorless polymer solution. The polymerizations produced high conversion and colorless polymers with moderately narrow polydispersity indices (PDI). The catalyst maintained high activity during the recycling experiments. We also investigated the kinetic and mechanistic behavior of these solid supported polymerization systems. Based on split kinetics experiments and UV-Vis studies it was believed that the activation and deactivation processes took place at the diffused hydrated interface between the solid support and organic phase. The branched (stars and graft) polymers were also synthesized using Na-clay supported catalyst. The produced polymers had narrow PDI and good initiator efficiencies. The functionality of the star polymers was confirmed using 1H NMR and dilute solution properties. The synthesis of graft-copolymer was confirmed by 1H NMR and atomic force microscopy. This thesis demonstrates the successful use of SAPC for ATRP to produce contamination free linear and branched polymers with moderately narrow PDI and high recycling efficiency.

Subjects/Keywords: ATRP; supported catalyst; surface mediation; compartmentalization; zero-order dependence; Polymer Chemistry

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APA (6^th Edition):

Aggarwal, R. (2010). Supported Aqueous-Phase Catalysis for Atom Transfer Radical Polymerization. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from http://trace.tennessee.edu/utk_graddiss/771

Chicago Manual of Style (16^th Edition):

Aggarwal, Ravi. “Supported Aqueous-Phase Catalysis for Atom Transfer Radical Polymerization.” 2010. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed June 23, 2018. http://trace.tennessee.edu/utk_graddiss/771.

MLA Handbook (7^th Edition):

Aggarwal, Ravi. “Supported Aqueous-Phase Catalysis for Atom Transfer Radical Polymerization.” 2010. Web. 23 Jun 2018.

Vancouver:

Aggarwal R. Supported Aqueous-Phase Catalysis for Atom Transfer Radical Polymerization. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2010. [cited 2018 Jun 23]. Available from: http://trace.tennessee.edu/utk_graddiss/771.

Council of Science Editors:

Aggarwal R. Supported Aqueous-Phase Catalysis for Atom Transfer Radical Polymerization. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2010. Available from: http://trace.tennessee.edu/utk_graddiss/771

Wright State University

2. Wytovich, Dominick Andrew. RESERVOIR ANALYSIS OF THE CLINTON INTERVAL IN STARK AND SUMMIT COUNTIES, OHIO.

Degree: MS, Earth and Environmental Sciences, 2010, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1276625315

► The characteristics of a sandstone reservoir of Silurian age (the “Clinton interval”) were analyzed using 382 geophysical well logs from the Dominion Gas storage field… (more)

Subjects/Keywords: Energy; Geology; Geophysics; Clinton; Isopach; Isolith; Stark; Summit; Reservoir Compartmentalization

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APA (6^th Edition):

Wytovich, D. A. (2010). RESERVOIR ANALYSIS OF THE CLINTON INTERVAL IN STARK AND SUMMIT COUNTIES, OHIO. (Masters Thesis). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1276625315

Chicago Manual of Style (16^th Edition):

Wytovich, Dominick Andrew. “RESERVOIR ANALYSIS OF THE CLINTON INTERVAL IN STARK AND SUMMIT COUNTIES, OHIO.” 2010. Masters Thesis, Wright State University. Accessed June 23, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright1276625315.

MLA Handbook (7^th Edition):

Wytovich, Dominick Andrew. “RESERVOIR ANALYSIS OF THE CLINTON INTERVAL IN STARK AND SUMMIT COUNTIES, OHIO.” 2010. Web. 23 Jun 2018.

Vancouver:

Wytovich DA. RESERVOIR ANALYSIS OF THE CLINTON INTERVAL IN STARK AND SUMMIT COUNTIES, OHIO. [Internet] [Masters thesis]. Wright State University; 2010. [cited 2018 Jun 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1276625315.

Council of Science Editors:

Wytovich DA. RESERVOIR ANALYSIS OF THE CLINTON INTERVAL IN STARK AND SUMMIT COUNTIES, OHIO. [Masters Thesis]. Wright State University; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1276625315

University of Toronto

3. Radford, Devon Richard. Understanding the Encapsulins:Prediction and Characterization of Phage Capsid-like Nanocompartments in Prokaryotes.

Degree: PhD, 2014, University of Toronto

URL: http://hdl.handle.net/1807/69450

► Encapsulins are a distinct family of prokaryotic compartments, comprised of an icosahedral shell encapsulating a variety of enzymes. The encapsulin shell protein is similar in… (more)

▼ Encapsulins are a distinct family of prokaryotic compartments, comprised of an icosahedral shell encapsulating a variety of enzymes. The encapsulin shell protein is similar in sequence and structure to the capsid protein of dsDNA tailed bacteriophages. Although found across diverse phyla of Bacteria and Archaea, few encapsulins were previously characterized. My thesis work utilized a bioinformatic approach to identify and investigate the functions of all encapsulins found in bacteria and Archaea. I found 590 encapsulins showing definite sequence similarity to the previously described encapsulins, which I refer to as "classical encapsulins". I identified four new enzyme families strongly predicted to be classical encapsulin cargo enzymes: ferredoxins, rubrerythrin-like, Dps-bacterioferritin-like, and hemerythrin-like proteins. While most species encode encapsulin cargo proteins adjacent to the encapsulin gene, I discovered 113 species encoding cargo proteins in genomic regions far removed from the encapsulin gene. I also found 118 genomes encoding two or three different enzymes strongly predicted to be targeted to the same encapsulin. Genes encoding three protein families, radical SAM oxidoreductases, CutA1-like proteins and metalloproteases, are highly enriched near encapsulin genes, but without targeting motifs for direct encapsulation. In addition to classical encapsulins, I discovered 1060 novel putative encapsulins related to diverse families of phage capsids. The largest family of these novel encapsulins, with 986 proteins is larger than the classical encapsulin family, and conserves a sulphur metabolism operon encoding cysteine desulfurases, acetyltransferases, and rhodaneses. These activities are predicted to relieve sulphur restriction, and toxicity of cyanide and other oxidants. Overall, novel and classical encapsulins and their encapsulated enzymes are predicted to contribute to polyvalent cation dependent catalytic activities, and relieve heavy metal and cyanide toxicity. Phylogenies predicted capsids and encapsulins share intermingled ancestry. My work shows that encapsulins perform more diverse functions and are much more widely distributed than any other prokaryotic compartment. Advisors/Committee Members: Davidson, Alan R, Parkinson, John, Molecular and Medical Genetics.

Subjects/Keywords: bacterial physiology; bacteriophage; compartmentalization; computational biology; encapsulin; 0307

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APA (6^th Edition):

Radford, D. R. (2014). Understanding the Encapsulins:Prediction and Characterization of Phage Capsid-like Nanocompartments in Prokaryotes. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/69450

Chicago Manual of Style (16^th Edition):

Radford, Devon Richard. “Understanding the Encapsulins:Prediction and Characterization of Phage Capsid-like Nanocompartments in Prokaryotes.” 2014. Doctoral Dissertation, University of Toronto. Accessed June 23, 2018. http://hdl.handle.net/1807/69450.

MLA Handbook (7^th Edition):

Radford, Devon Richard. “Understanding the Encapsulins:Prediction and Characterization of Phage Capsid-like Nanocompartments in Prokaryotes.” 2014. Web. 23 Jun 2018.

Vancouver:

Radford DR. Understanding the Encapsulins:Prediction and Characterization of Phage Capsid-like Nanocompartments in Prokaryotes. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/1807/69450.

Council of Science Editors:

Radford DR. Understanding the Encapsulins:Prediction and Characterization of Phage Capsid-like Nanocompartments in Prokaryotes. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/69450

Queens University

4. Truong, Tammy. Compartmentalized phosphodiesterase 4D isoforms expression, targeting and localization in vascular myocytes .

Degree: Pathology & Molecular Medicine, 2014, Queens University

URL: http://hdl.handle.net/1974/8658

► During the development of atherosclerosis, contractile vascular smooth muscle cells (VSMCs) change to cells capable of migrating and proliferating to mediate repair, where the responses… (more)

▼ During the development of atherosclerosis, contractile vascular smooth muscle cells (VSMCs) change to cells capable of migrating and proliferating to mediate repair, where the responses may be adaptive or mal-adaptive in effect. Cyclic adenosine monophosphate (cAMP)-elevating agents have been shown to inhibit migration of VSMC. cAMP activity within the cell is known to be ubiquitous and dynamic, requiring control through signal termination mechanisms for cellular homeostasis. Phosphodiesterase (PDE) enzymes are central to this critical regulatory process catalyzing the hydrolysis of cAMP. A great deal of insight into the role of PDEs in defining compartmentalization of cAMP signaling has arisen predominately from recent studies on the cAMP-specific PDE4 family. Compartmentalization of PDE4 is mediated by their unique N-terminal domains, which have been proposed to provide the “postcodes/zipcodes” for cellular localization. PDE4D isoforms vary widely, yet their conservation over evolutionary time suggests important non-redundant roles in distinct cellular processes. To study the potential role of individual PDE4D isoforms we seek to utilize the unique N-terminal targeting domains that are proposed to be responsible for their protein-protein interactions and site-directed localization. Herein, we report on the expression, targeting and localization of five “long” PDE4D isoforms and the impact on cell morphology of certain amino-terminal domains of individual PDE4D constructs expressing green fluorescent protein (NT-PDE4D/GFP) in human aortic smooth muscle cells (HASMCs). Through the development of engineered NT-PDE4D/GFP expression plasmids, we were able to study the cell biological impacts associated with the overexpression of individual PDE4D amino-terminal variants in HASMCs. We show that NT-PDE4D5/GFP and NT-PDE4D7/GFP expressing cells exhibited an elongated cell morphology, where this effect was much more marked in NT-PDE4D7/GFP expressing cells, exhibiting multiple leading edge structures and highly elongated “tails”. We identify a potential role for PDE4D7 targeting in the regulation of cell polarity and migration. Our results suggest the novel idea that PDE4D7, rather than the four other long PDE4D isoforms (PDE4D3, PDE4D5, PDE4D8, or PDE4D9), represents the dominant PDE4D variant involved in controlling cAMP-mediated effects on cell tail retraction dynamics.

Subjects/Keywords: cell migration; vascular smooth muscle cell; cAMP; compartmentalization; phosphodiesterase 4D

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APA (6^th Edition):

Truong, T. (2014). Compartmentalized phosphodiesterase 4D isoforms expression, targeting and localization in vascular myocytes . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/8658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Truong, Tammy. “Compartmentalized phosphodiesterase 4D isoforms expression, targeting and localization in vascular myocytes .” 2014. Thesis, Queens University. Accessed June 23, 2018. http://hdl.handle.net/1974/8658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Truong, Tammy. “Compartmentalized phosphodiesterase 4D isoforms expression, targeting and localization in vascular myocytes .” 2014. Web. 23 Jun 2018.

Vancouver:

Truong T. Compartmentalized phosphodiesterase 4D isoforms expression, targeting and localization in vascular myocytes . [Internet] [Thesis]. Queens University; 2014. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/1974/8658.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Truong T. Compartmentalized phosphodiesterase 4D isoforms expression, targeting and localization in vascular myocytes . [Thesis]. Queens University; 2014. Available from: http://hdl.handle.net/1974/8658

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

5. Strulson, Christopher A. toward understanding RNA structure, folding, and function in cells.

Degree: PhD, Chemistry, 2014, Penn State University

URL: https://etda.libraries.psu.edu/catalog/20143

► Ever since the discovery of RNA as an integral biomolecule in cellular function, the principles governing RNA folding and function have been studied. These studies… (more)

▼ Ever since the discovery of RNA as an integral biomolecule in cellular function, the principles governing RNA folding and function have been studied. These studies have primarily focused on the thermodynamics and kinetics of RNA folding in conditions that are folding-favorable and non-physiological including dilute solution with relatively high ionic strength and Mg2+ ion concentrations. While these studies have imparted invaluable knowledge towards the understanding of RNA biophysics, there is still a great need to characterize RNA folding and function in conditions that are much closer to those of a cell; however, because of the intricacies of the cellular environment, elucidating meaningful biophysical properties from experiments directly in living cells remains difficult. In an effort to capture the effects of this complex environment on RNA folding, in vitro experiments focusing on distinct features of the cellular environment are performed in attempt to learn how these specific properties of the cellular matrix impact RNA. In this thesis, RNA structure, folding, and function are assessed from both a thermodynamic and kinetic prospective under conditions that mimic those of cells. In particular, effects of compartmentalization, macromolecular crowding, and low molecular cosolutes are examined in terms of RNA catalysis and RNA folding using an array of techniques including RNA partitioning, ribozyme kinetics, small angle X-ray scattering (SAXS), structure mapping methods, and RNA folding thermodynamics. One key aspect of cells is their ability to compartmentalize biomolecules; thus, allowing them to control local concentrations of these molecules. In Chapter Two and Three of this thesis, it is revealed that local concentrations of RNA can be controlled using an aqueous two-phase system composed of two high molecular weight polymers. Additionally, increasing the local concentration of an enzyme strand of a two-piece ribozyme within one of the aqueous phase compartments, leads to a significant enhancement in catalysis under single-turnover conditions. This work indicates that compartmentalization could be very important for RNA function in modern cells as well as in RNA regulation and activation in early-earth protocells. Another key factor of the cellular environment is the concentration of free Mg2+, which is only ~0.5 mM and ~2 mM in eukaryotic and prokaryotic cells, respectively. These values are considerably lower than the free Mg2+ (~10 mM) typically used in RNA structure and folding studies in the literature. Additionally, there are high concentrations of molecules (both high- and low-molecular weight) inside cells that can interact with RNA, exclude volume, and alter solvent conditions. These molecules can have large impacts on RNA structure, folding, and function especially when Mg2+ concentrations are physiological. It is revealed in Chapter Four of this thesis that the self-cleavage of a small catalytic RNA, the cytoplasmic polyadenylation element–binding protein 3 HDV-like ribozyme (CPEB3), is…

Subjects/Keywords: RNA; ribozyme; molecular crowding; macromolecular crowding; catalysis; compartmentalization; folding cooperativity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Strulson, C. A. (2014). toward understanding RNA structure, folding, and function in cells. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/20143

Chicago Manual of Style (16^th Edition):

Strulson, Christopher A. “toward understanding RNA structure, folding, and function in cells.” 2014. Doctoral Dissertation, Penn State University. Accessed June 23, 2018. https://etda.libraries.psu.edu/catalog/20143.

MLA Handbook (7^th Edition):

Strulson, Christopher A. “toward understanding RNA structure, folding, and function in cells.” 2014. Web. 23 Jun 2018.

Vancouver:

Strulson CA. toward understanding RNA structure, folding, and function in cells. [Internet] [Doctoral dissertation]. Penn State University; 2014. [cited 2018 Jun 23]. Available from: https://etda.libraries.psu.edu/catalog/20143.

Council of Science Editors:

Strulson CA. toward understanding RNA structure, folding, and function in cells. [Doctoral Dissertation]. Penn State University; 2014. Available from: https://etda.libraries.psu.edu/catalog/20143

Cornell University

6. Forster, Brian. Regulation Of The Metalloprotease Of Listeria Monocytogenes During Intracellular Infection .

Degree: 2011, Cornell University

URL: http://hdl.handle.net/1813/30617

► Listeria monocytogenes is a Gram-positive, facultative intracellular bacterial pathogen that multiplies in the cytosol of host cells and relies on an actin-based mechanism of motility… (more)

▼ Listeria monocytogenes is a Gram-positive, facultative intracellular bacterial pathogen that multiplies in the cytosol of host cells and relies on an actin-based mechanism of motility to spread from cell-to-cell without exiting the intracellular milieu. Virulence of L. monocytogenes is related to its ability to escape from vacuoles formed upon initial entry into a host cell and during cell-to-cell spread. One factor contributing to escape from vacuoles is a broad-range phospholipase C (PC-PLC). PC-PLC is translocated across the bacterial membrane as an inactive proenzyme and accumulates in the periplasmic space. PC-PLC maturation is dependent upon a decrease in pH and on the metalloprotease of L. monocytogenes (Mpl). Mpl, a thermolysin-like protease, is produced as a zymogen and matures via intramolecular autocatalysis. In this study, we wished to investigate the regulation of Mpl during intracellular infection. Our results show that at physiological pH, the zymogen remains bacterium-associated, presumably in the periplasm. Mpl produced in absence of its propeptide is aberrantly secreted suggesting that the propeptide regulates compartmentalization. Upon a decrease in pH, the bacterium-associated zymogen undergoes autocatalysis and mediates the proteolytic activation of PC-PLC. Mature Mpl and PC-PLC are then rapidly secreted across the cell wall. Two amino acids in the catalytic domain of Mpl, H226 and H241, influence mature Mpl secretion and its ability to process PC-PLC. The compartmentalization of the zymogen is also influenced by PrsA2, a peptidyl prolyl cis-trans isomerase that is anchored by an acyl chain to the trans side of the membrane. In absence of PrsA2, the proforms of Mpl and PC-PLC are aberrantly secreted across the cell wall and no longer undergo maturation upon a decrease in pH. Taken together, these results indicate that PrsA2 and the propeptide of Mpl serve to maintain the association of Mpl with bacteria and that this compartmentalization is required for Mpl to undergo autocatalysis at acidic pH and to mediate the proteolytic activation of PC-PLC. Advisors/Committee Members: Scidmore, Marci (committeeMember), Wilson, David B (committeeMember).

Subjects/Keywords: Listeria monocytogenes; metalloprotease; proproteins; compartmentalization; pH; peptidyl-prolyl cis/trans isomerase

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APA (6^th Edition):

Forster, B. (2011). Regulation Of The Metalloprotease Of Listeria Monocytogenes During Intracellular Infection . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/30617

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Forster, Brian. “Regulation Of The Metalloprotease Of Listeria Monocytogenes During Intracellular Infection .” 2011. Thesis, Cornell University. Accessed June 23, 2018. http://hdl.handle.net/1813/30617.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Forster, Brian. “Regulation Of The Metalloprotease Of Listeria Monocytogenes During Intracellular Infection .” 2011. Web. 23 Jun 2018.

Vancouver:

Forster B. Regulation Of The Metalloprotease Of Listeria Monocytogenes During Intracellular Infection . [Internet] [Thesis]. Cornell University; 2011. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/1813/30617.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Forster B. Regulation Of The Metalloprotease Of Listeria Monocytogenes During Intracellular Infection . [Thesis]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30617

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

7. Shin, KWANG SUP. Biomechanics of Compartmentalized Mechanical Properties of Extraocular Muscle and Tendon.

Degree: Mechanical Engineering, 2014, UCLA

URL: http://www.escholarship.org/uc/item/0fx3w530

► A controversial issue in the eye movement field involves the compartmentalization of extraocular muscle (EOM). Conventionally, it has been believed that each individual EOM is… (more)

▼ A controversial issue in the eye movement field involves the compartmentalization of extraocular muscle (EOM). Conventionally, it has been believed that each individual EOM is innervated uniformly by its motor nerve, and behaves uniformly. However, recent studies have revealed that motor nerves innervating horizontal rectus EOMs are bifurcated into two divisions that control, respectively, the superior and inferior zones of muscle fibers. This finding has motivated the proposition that each of the two compartments of these EOMs might be controlled independently, so that each EOM could behave as it were two parallel but independent actuators. The compartmental independence postulate requires a mechanically low degree of transverse force coupling among parallel EOM and extraocular tendon (EOT) fibers. This project aimed to employ biomechanical characterization to EOM and EOT to test the plausibility of this hypothesis.The first study described the biomechanical characterization of EOT, in both at the level of individual fibers and whole tendon. Nano indentation of EOT fiber bundles analyzed within the Hertzian framework effectively characterized the transverse Young's modulus for bovine EOT fiber bundles using atomic force microscopy (AFM). A precise estimate of the Poisson ratio (PR) of bovine EOTs was calculated using optical coherence tomography (OCT) during tensile loading. The second study investigated mechanical potential for independent compartmental behavior in EOM and EOT. Extensive experimental study was performed to examine the passive mechanical interaction between sets compartments using a custom fabricated dual channel micro-tensile load cell. Independent active contractile behavior of EOM compartments was also examined using calcium-induced EOM contraction. Both passive loading and active contraction findings indicate that EOM and tendon have a high degree of biomechanical independence, sufficient to support the proposed functional diversity of actions in distinct neuromuscular compartments of the horizontal rectus EOMs.Finally, biomechanical effects of Z-tenotomy and Z-myotomy, surgical techniques employing transverse incisions for EOT or EOM weakening to correct strabismus (misalignment of the eyes), were characterized using tensile loading. Both Z-tenotomy and Z-myotomy demonstrated minimal shear force coupling, which confirms and extends the findings of independent compartmental behavior in second study, and explains the surgical effects of the procedure. Additional viscoelastic characterization was performed using EOM specimens.

Subjects/Keywords: Mechanical engineering; Biomechanics; Biomedical engineering; Biomechanics; Compartmentalization; Extraocular Muscle; Extraocular Tendon

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APA (6^th Edition):

Shin, K. S. (2014). Biomechanics of Compartmentalized Mechanical Properties of Extraocular Muscle and Tendon. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/0fx3w530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shin, KWANG SUP. “Biomechanics of Compartmentalized Mechanical Properties of Extraocular Muscle and Tendon.” 2014. Thesis, UCLA. Accessed June 23, 2018. http://www.escholarship.org/uc/item/0fx3w530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shin, KWANG SUP. “Biomechanics of Compartmentalized Mechanical Properties of Extraocular Muscle and Tendon.” 2014. Web. 23 Jun 2018.

Vancouver:

Shin KS. Biomechanics of Compartmentalized Mechanical Properties of Extraocular Muscle and Tendon. [Internet] [Thesis]. UCLA; 2014. [cited 2018 Jun 23]. Available from: http://www.escholarship.org/uc/item/0fx3w530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shin KS. Biomechanics of Compartmentalized Mechanical Properties of Extraocular Muscle and Tendon. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/0fx3w530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

8. Le bars, Romain. Morphogenèse de compartiments membranaires : formation de l'autophagosome chez les plantes : Morphogenesis of membranar compartments : autophagosome formation in plants.

Degree: Docteur es, Biologie, 2013, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2013PA112349

►

L'autophagie est un processus permettant la dégradation de constituants cytosoliques dans un compartiment lytique, par leur séquestration au sein d'une vésicule à double membrane :… (more)

▼

L'autophagie est un processus permettant la dégradation de constituants cytosoliques dans un compartiment lytique, par leur séquestration au sein d'une vésicule à double membrane : l'autophagosome. L'autophagie est, avec la voie ubiquitine-protéasome, l'une des deux grandes voies de dégradation présente de manière fortement conservée chez les cellules eucaryotes. Présente à un niveau basal, elle peut être stimulée afin de permettre la remobilisation de ressources cellulaires, ou d'assurer des fonctions cytoprotectrices et de détoxification. La formation d'autophagosomes traduit alors la capacité du système endomembranaire à s'adapter aux besoins cellulaires. Cependant, la mécanique membranaire et moléculaire de ce phénomène reste mal comprise. L'objectif de ce travail de thèse était de mieux comprendre la formation de ce compartiment dans la cellule végétale. Pour cela, nous avons tout d'abord mis au point les conditions propices à l'étude de l'autophagie dans la racine d’Arabidopsis thaliana, puis nous avons entrepris l'identification de marqueurs des étapes de formation de l'autophagosome. L'étude par imagerie en temps réel et 3D de la protéine ATG5, impliquée dans l’expansion membranaire, nous a permis de mettre en évidence son recrutement transitoire sur un domaine particulier de l'autophagosome en formation, son ouverture. De plus, l'étude de différents acteurs du système endomembranaire, nous a permis de mettre en évidence et de caractériser l'implication du réticulum endoplasmique et de ATG9, pour aboutir à un modèle de la formation de l'autophagosome chez les plantes.

Autophagy is a catabolic process targeting cytosolic compounds to the lytic compartment after sequestration within a double membrane bound vesicle: the autophagosome. Along with the ubiquitin-proteasome pathway, autophagy is one of the main catabolic processes conserved among eukaryotic cells. Present at a basal level, it can be stimulated to allow: remobilization of cell resources, cytoprotective functions, and detoxification. Autophagosome formation demonstrates the capacity of the endomembrane system to adapt dynamically to the cell's environment. However, the membrane and molecular processes involved are still poorly understood. This work aimed to advance understanding of autophagosome formation in plant cells. First of all, we set up suitable conditions for the study of autophagy in the Arabidopsis root, then we identified markers of the autophagosome formation steps. Live and 3D imaging of the ATG5 protein, involved in membrane expansion, demonstrated its transient recruitment to a specific domain of the forming autophagosome, its aperture. Furthermore, studying different actors of the endomembrane system has allowed us to implicate the endoplasmic reticulum and ATG9, and to establish a model for autophagosome formation in plants.

Advisors/Committee Members: Satiat-Jeunemaître, Béatrice (thesis director).

Subjects/Keywords: Autophagosome; Autophagie; Compartimentation cellulaire; Autophagosome; Autophagy; Cell compartmentalization

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APA (6^th Edition):

Le bars, R. (2013). Morphogenèse de compartiments membranaires : formation de l'autophagosome chez les plantes : Morphogenesis of membranar compartments : autophagosome formation in plants. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2013PA112349

Chicago Manual of Style (16^th Edition):

Le bars, Romain. “Morphogenèse de compartiments membranaires : formation de l'autophagosome chez les plantes : Morphogenesis of membranar compartments : autophagosome formation in plants.” 2013. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed June 23, 2018. http://www.theses.fr/2013PA112349.

MLA Handbook (7^th Edition):

Le bars, Romain. “Morphogenèse de compartiments membranaires : formation de l'autophagosome chez les plantes : Morphogenesis of membranar compartments : autophagosome formation in plants.” 2013. Web. 23 Jun 2018.

Vancouver:

Le bars R. Morphogenèse de compartiments membranaires : formation de l'autophagosome chez les plantes : Morphogenesis of membranar compartments : autophagosome formation in plants. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2013. [cited 2018 Jun 23]. Available from: http://www.theses.fr/2013PA112349.

Council of Science Editors:

Le bars R. Morphogenèse de compartiments membranaires : formation de l'autophagosome chez les plantes : Morphogenesis of membranar compartments : autophagosome formation in plants. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2013. Available from: http://www.theses.fr/2013PA112349

Kansas State University

9. Evans, Drew W. The compartmentalization and biomarker analysis of the spivey-grabs-basil field, south-central Kansas.

Degree: MS, Department of Geology, 2011, Kansas State University

URL: http://hdl.handle.net/2097/8785

► The Spivey-Grabs-Basil oil field is a highly developed field in south-central Kansas, having large variability in its production and in the Pineville Tripolite facies. The… (more)

▼ The Spivey-Grabs-Basil oil field is a highly developed field in south-central Kansas, having large variability in its production and in the Pineville Tripolite facies. The Pineville Tripolite is the primary producing formation of this field having major isopach variations, possibly influencing production. The hypothesis that the field is highly compartmentalized is from the varied production, isopach and structure of the field. This study investigated the Pineville Tripolite facies in the Spivey-Grabs-Basil Oil Field, with the Basil area the predominant focus, and its possible compartmentalization by looking at the gas chromatograms and their biomarker signatures. This field has had several studies investigating the geophysical attributes, depositional setting and large-scale compartmentalization. Post depositional sea-level changes and possibly syntectonics exposed the Reeds Spring to a sub-aerial environment where meteoric alteration created immense porosity and the Pineville Tripolite facies. Geochemical data shows evidence that this section of the field is sourced from both a marine shale and carbonate source at peak oil maturity, deposited in an anoxic environment. Biodegradation appears very slight, with most alterations transpiring in the alkane ranges only, leaving all other susceptible hydrocarbons unaltered. Compartments within the field are harder to identify when comparing geological data to oil data. Isopach data shows altered thickness of the Pineville Tripolite from well to well, as do Pineville structure values. The isopach and structural data point to possible areas for compartments, but it is from oil geochemical data that compartments become more visible. API gravities and GOR show motley values, but do indicate two significant areas of segregation. The deepest, most southern end of the study showed lighter gravity oils than the middle, suggesting possible fill and spill between the two. However, biomarker abundance indicates three possible compartments. The southern compartment has many more biomarker volumes than do the middle compartment, both divided by a reservoir pinch-out. The third most northeastern well has high biomarker abundance, but shows no geological separators from the other wells. Production from this field may be improved by investigating the biomarkers to allocate these compartments and possible barriers close to wells. Advisors/Committee Members: Matthew W. Totten.

Subjects/Keywords: Compartmentalization; Biomarkers; Geology; Geochemistry; Geochemistry (0996); Geology (0372)

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APA (6^th Edition):

Evans, D. W. (2011). The compartmentalization and biomarker analysis of the spivey-grabs-basil field, south-central Kansas. (Masters Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/8785

Chicago Manual of Style (16^th Edition):

Evans, Drew W. “The compartmentalization and biomarker analysis of the spivey-grabs-basil field, south-central Kansas.” 2011. Masters Thesis, Kansas State University. Accessed June 23, 2018. http://hdl.handle.net/2097/8785.

MLA Handbook (7^th Edition):

Evans, Drew W. “The compartmentalization and biomarker analysis of the spivey-grabs-basil field, south-central Kansas.” 2011. Web. 23 Jun 2018.

Vancouver:

Evans DW. The compartmentalization and biomarker analysis of the spivey-grabs-basil field, south-central Kansas. [Internet] [Masters thesis]. Kansas State University; 2011. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/2097/8785.

Council of Science Editors:

Evans DW. The compartmentalization and biomarker analysis of the spivey-grabs-basil field, south-central Kansas. [Masters Thesis]. Kansas State University; 2011. Available from: http://hdl.handle.net/2097/8785

Texas A&M University

10. Alfaleh, Abdulhamed A. Use of Topological Data Analysis in Reservoir Engineering: Application to Inverted 4D Seismic Data.

Degree: 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/154176

► Data analysis is one of the most important topics in any industry. In petroleum engineering, the complexity of reservoir data presents a challenge for engineers… (more)

Subjects/Keywords: Topological Data Analysis; Inverted 4D Seismic; Reservoirs connectivity; compartmentalization

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APA (6^th Edition):

Alfaleh, A. A. (2014). Use of Topological Data Analysis in Reservoir Engineering: Application to Inverted 4D Seismic Data. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/154176

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alfaleh, Abdulhamed A. “Use of Topological Data Analysis in Reservoir Engineering: Application to Inverted 4D Seismic Data.” 2014. Thesis, Texas A&M University. Accessed June 23, 2018. http://hdl.handle.net/1969.1/154176.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alfaleh, Abdulhamed A. “Use of Topological Data Analysis in Reservoir Engineering: Application to Inverted 4D Seismic Data.” 2014. Web. 23 Jun 2018.

Vancouver:

Alfaleh AA. Use of Topological Data Analysis in Reservoir Engineering: Application to Inverted 4D Seismic Data. [Internet] [Thesis]. Texas A&M University; 2014. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/1969.1/154176.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alfaleh AA. Use of Topological Data Analysis in Reservoir Engineering: Application to Inverted 4D Seismic Data. [Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/154176

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Catholique de Louvain

11. Woronoff, Gabrielle. Development of methods for the directed evolution of penicillin G acylase by in vitro compartmentalization.

Degree: 2012, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/110274

►

Enzymatic catalysts have been finely tuned for their specific function though natural evolution, by iterative mutations and selections cycles. Directed evolution mimics natural evolution at… (more)

▼

Enzymatic catalysts have been finely tuned for their specific function though natural evolution, by iterative mutations and selections cycles. Directed evolution mimics natural evolution at the laboratory scale and allows the selection, in large collections of variants, of enzymes bearing new properties. In vitro compartmentalization (IVC) is the directed evolution technique used in this work, and consists of aqueous microdroplets dispersed in oil acting as artificial cells. Single genes can be encapsulated in droplets together with the proteins encoded by this gene and substrates to test its enzymatic activity. These compartments can be easily manipulated using microfluidic techniques. Two fluorogenic enzymatic tests applied to the detection of the penicillin G acylase in droplets were developed. The retention of coumarin compounds in microdroplets was evaluated and a collection of substrates with a sulfonated coumarin as leaving group was synthesized. Libraries of mutants of penicillin acylase were created and screened for variants that would have acquired broader substrate specificity compared to the wild type enzyme. The second enzymatic test called AFET (for Activity Fed Enzyme Translation) is based on the in vitro translation of proteins. It consists of the linkage between the enzymatic activity of the enzyme of interest with its own in vitro translation together with the translation of a reporter gene, in our case the GFP. The presented work lays a solid foundation for future work in the directed evolution of penicillin acylase and other enzymes of interest.

Des catalyseurs enzymatiques aux paramètres finement ajustés pour leur fonction spécifique ont été développés au cours de l’évolution par cycles itératifs de mutations et sélections. L’évolution dirigée mime l’évolution naturelle à l’échelle du laboratoire afin de trouver au sein de larges collections de variants, des enzymes présentant des propriétés nouvelles. La compartimentalisation in vitro (IVC), technique d’évolution dirigée utilisée dans ce travail, consiste à utiliser des gouttes aqueuses dispersées dans une phase huileuse comme cellules artificielles. Il est possible d’encapsuler au sein d’une même goutte un gène, la protéine qu’il encode et d’éventuels substrats permettant de tester son activité. Ces compartiments peuvent ensuite être facilement manipulés par techniques microfluidiques. Deux tests enzymatiques fluorogéniques applicables à la détection de l’activité de la pénicilline G acylase en gouttes ont été développés. La rétention de composés coumarinés au sein des microcompartiments d’émulsion a été évaluée et une collection de substrats possédant une coumarine sulfonate comme groupe partant a été synthétisée. Des banques de mutants de la pénicilline acylase ont été créées et criblées en IVC afin de trouver des variants de l’enzyme possédant une spécificité de substrat plus large que l’enzyme sauvage. Le second test enzymatique appelé « AFET » (pour Activity Fed Enzyme Translation), et basé sur la traduction protéique in vitro, a…

Advisors/Committee Members: UCL - SST/ISV/ISV - Institut des sciences de la vie, Soumillion, Patrice, Remacle, Claude, Fastrez, Jacques, Elias, Benjamin, Janssen, Dick B., Griffiths, Andrew.

Subjects/Keywords: Directed evolution; Penicillin G acylase; Dropletb-based microfluidics; In vitro compartmentalization

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APA (6^th Edition):

Woronoff, G. (2012). Development of methods for the directed evolution of penicillin G acylase by in vitro compartmentalization. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/110274

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Woronoff, Gabrielle. “Development of methods for the directed evolution of penicillin G acylase by in vitro compartmentalization.” 2012. Thesis, Université Catholique de Louvain. Accessed June 23, 2018. http://hdl.handle.net/2078.1/110274.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Woronoff, Gabrielle. “Development of methods for the directed evolution of penicillin G acylase by in vitro compartmentalization.” 2012. Web. 23 Jun 2018.

Vancouver:

Woronoff G. Development of methods for the directed evolution of penicillin G acylase by in vitro compartmentalization. [Internet] [Thesis]. Université Catholique de Louvain; 2012. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/2078.1/110274.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Woronoff G. Development of methods for the directed evolution of penicillin G acylase by in vitro compartmentalization. [Thesis]. Université Catholique de Louvain; 2012. Available from: http://hdl.handle.net/2078.1/110274

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

12. Pepermans, R.A. Cytoskeleton induced partitioning of the plasma membrane: The picket-fence model and the compartmentalized plasma membrane theory.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/256468

► The development of single-particle tracking has allowed membrane researchers to observe individual movements of membrane molecules, thereby gaining new insights into the dynamics of the… (more)

Subjects/Keywords: single-particle tracking; membrane-skeleton fence model; anchored-protein picket model; hop diffusion; compartmentalization.

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APA (6^th Edition):

Pepermans, R. A. (2012). Cytoskeleton induced partitioning of the plasma membrane: The picket-fence model and the compartmentalized plasma membrane theory. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/256468

Chicago Manual of Style (16^th Edition):

Pepermans, R A. “Cytoskeleton induced partitioning of the plasma membrane: The picket-fence model and the compartmentalized plasma membrane theory.” 2012. Masters Thesis, Universiteit Utrecht. Accessed June 23, 2018. http://dspace.library.uu.nl:8080/handle/1874/256468.

MLA Handbook (7^th Edition):

Pepermans, R A. “Cytoskeleton induced partitioning of the plasma membrane: The picket-fence model and the compartmentalized plasma membrane theory.” 2012. Web. 23 Jun 2018.

Vancouver:

Pepermans RA. Cytoskeleton induced partitioning of the plasma membrane: The picket-fence model and the compartmentalized plasma membrane theory. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2018 Jun 23]. Available from: http://dspace.library.uu.nl:8080/handle/1874/256468.

Council of Science Editors:

Pepermans RA. Cytoskeleton induced partitioning of the plasma membrane: The picket-fence model and the compartmentalized plasma membrane theory. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/256468

Penn State University

13. Molden, Rosalynn Conniff. Enhancing RNA catalysis through compartmentalization.

Degree: MS, Chemistry, 2009, Penn State University

URL: https://etda.libraries.psu.edu/catalog/10057

► Experimental models of cellular conditions are useful because they can be used to study cell processes in a controlled environment and because they can be… (more)

Subjects/Keywords: model cell; aqueous two-phase systems; hammerhead ribozyme kinetics; RNA; cellular compartmentalization

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APA (6^th Edition):

Molden, R. C. (2009). Enhancing RNA catalysis through compartmentalization. (Masters Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/10057

Chicago Manual of Style (16^th Edition):

Molden, Rosalynn Conniff. “Enhancing RNA catalysis through compartmentalization.” 2009. Masters Thesis, Penn State University. Accessed June 23, 2018. https://etda.libraries.psu.edu/catalog/10057.

MLA Handbook (7^th Edition):

Molden, Rosalynn Conniff. “Enhancing RNA catalysis through compartmentalization.” 2009. Web. 23 Jun 2018.

Vancouver:

Molden RC. Enhancing RNA catalysis through compartmentalization. [Internet] [Masters thesis]. Penn State University; 2009. [cited 2018 Jun 23]. Available from: https://etda.libraries.psu.edu/catalog/10057.

Council of Science Editors:

Molden RC. Enhancing RNA catalysis through compartmentalization. [Masters Thesis]. Penn State University; 2009. Available from: https://etda.libraries.psu.edu/catalog/10057

14. Harris, Garrett R. Evaluation of Groundwater Quality in Paleozoic Aquifers Using GIS Techniques, Central Texas.

Degree: MS- Geology, Geology, 2016, Stephen F. Austin State University

URL: http://scholarworks.sfasu.edu/etds/45

► Lab analyses of groundwater chemistry from monitoring wells completed in the Paleozoic Aquifers surrounding the Llano Uplift of Central Texas has been recorded and… (more)

Subjects/Keywords: Hydraulic Connectivity; Nested Pairs; Piper Diagrams; Compartmentalization; Groundwater; Aquifer; Earth Sciences; Geochemistry; Geology; Hydrology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harris, G. R. (2016). Evaluation of Groundwater Quality in Paleozoic Aquifers Using GIS Techniques, Central Texas. (Masters Thesis). Stephen F. Austin State University. Retrieved from http://scholarworks.sfasu.edu/etds/45

Chicago Manual of Style (16^th Edition):

Harris, Garrett R. “Evaluation of Groundwater Quality in Paleozoic Aquifers Using GIS Techniques, Central Texas.” 2016. Masters Thesis, Stephen F. Austin State University. Accessed June 23, 2018. http://scholarworks.sfasu.edu/etds/45.

MLA Handbook (7^th Edition):

Harris, Garrett R. “Evaluation of Groundwater Quality in Paleozoic Aquifers Using GIS Techniques, Central Texas.” 2016. Web. 23 Jun 2018.

Vancouver:

Harris GR. Evaluation of Groundwater Quality in Paleozoic Aquifers Using GIS Techniques, Central Texas. [Internet] [Masters thesis]. Stephen F. Austin State University; 2016. [cited 2018 Jun 23]. Available from: http://scholarworks.sfasu.edu/etds/45.

Council of Science Editors:

Harris GR. Evaluation of Groundwater Quality in Paleozoic Aquifers Using GIS Techniques, Central Texas. [Masters Thesis]. Stephen F. Austin State University; 2016. Available from: http://scholarworks.sfasu.edu/etds/45

15. Gabelini, Beatriz Marques [UNESP]. Suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP): Análise fisiográfica e quantitativa.

Degree: 2017, Universidade Estadual Paulista

URL: http://hdl.handle.net/11449/151503

►

Os escorregamentos são movimentos de massa recorrentes na Serra do Mar. Quando ocorrem de forma generalizada podem causar prejuízos e perdas de vidas, como no… (more)

▼

Os escorregamentos são movimentos de massa recorrentes na Serra do Mar. Quando ocorrem de forma generalizada podem causar prejuízos e perdas de vidas, como no acidente ocorrido no município de Caraguatatuba (SP) em 1967. Com o intuito de evitar estes danos, na literatura, são propostos vários métodos para analisar e prever a ocorrência destes processos. Diante do exposto, o objetivo principal desta pesquisa consiste em analisar a suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP), utilizando a compartimentação fisiográfica e a análise quantitativa. Para alcançar estes objetivos foi realizada a análise e compartimentação do terreno e a análise quantitativa dos fatores condicionantes (declividade, hipsometria e geologia) e da compartimentação fisiográfica. Foram testados, em ambiente SIG, diversos cenários a partir de dois métodos distintos. No Método 1, os fatores condicionantes foram analisados através de pesos de influência. No Método 2, os fatores condicionantes mantiveram os mesmos pesos do Método 1 e foram somados pesos para as classes de cada fator. A compartimentação fisiográfica definiu 16 unidades. Os resultados demonstraram que a maioria dos escorregamentos se concentraram na unidade fisiográfica IV e que há um controle topográfico na distribuição dos escorregamentos entre 100 e 400 m de altitude, em encostas com declividade entre 15º e 30º, compostas por gnaisse migmatítico e granito-gnaisse. Os mapas elaborados a partir do Método 2 apresentaram expressiva concordância com o mapeamento de cicatrizes de Fúlfaro et al. (1976). O cenário escolhido como mais coerente foi o Cenário O, em que houve 60% de influência da declividade, 30% de hipsometria e 10% de geologia. Portanto, a partir do estudo da suscetibilidade, por meio da análise fisiográfica e quantitativa, é possível afirmar que o fator que tem mais influência na ocorrência de escorregamentos, na área de estudo, é a declividade, seguida da hipsometria e geologia.

Landslides are recurrent mass movements in the Serra do Mar. When they occur in a generalized way, as in the event occurred in Caraguatatuba city (SP) in 1967, they can cause a lot of environmental damage and deaths. In order to avoid this damage, several methods were proposed to analyze and predict the occurrence of this process. Considering the above, the main objective of this research is to analyze the susceptibility to landslides in the region between Caraguatatuba and São Sebastião (SP), using physiographic compartmentalization and quantitative analysis. In order to achieve these objectives, a physiographic analysis of the study area was carried out by the method of physiographic compartmentalization. Topographic and geological factors, as well as a physiographic comparison were analyzed using percentages of Class Frequency, Scars Concentration and Disproportionality Factor. We tested several scenarios from two different methods in a GIS environment. Method 1 by which the conditioning factors were analyzed through influence weights and Method 2, which…

Advisors/Committee Members: Reis, Fábio Augusto Gomes Vieira [UNESP], Universidade Estadual Paulista (UNESP).

Subjects/Keywords: escorregamentos; suscetibilidade; compartimentação fisiográfica; análise quantitativa; SIG; Landslides; Susceptibility analysis; Quantitative analysis; Physiographic compartmentalization; GIS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gabelini, B. M. [. (2017). Suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP): Análise fisiográfica e quantitativa. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/151503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gabelini, Beatriz Marques [UNESP]. “Suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP): Análise fisiográfica e quantitativa.” 2017. Thesis, Universidade Estadual Paulista. Accessed June 23, 2018. http://hdl.handle.net/11449/151503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gabelini, Beatriz Marques [UNESP]. “Suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP): Análise fisiográfica e quantitativa.” 2017. Web. 23 Jun 2018.

Vancouver:

Gabelini BM[. Suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP): Análise fisiográfica e quantitativa. [Internet] [Thesis]. Universidade Estadual Paulista; 2017. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/11449/151503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gabelini BM[. Suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP): Análise fisiográfica e quantitativa. [Thesis]. Universidade Estadual Paulista; 2017. Available from: http://hdl.handle.net/11449/151503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Rasid, Orhan. NK cells and systemic inflammation : compartmentalization and memory responses : Cellules NK et inflammation systémique : compartimentalisation et réponse mémoire.

Degree: Docteur es, Immunologie, 2016, Sorbonne Paris Cité

URL: http://www.theses.fr/2016USPCB078

►

L'inflammation systémique est une réaction qui implique l’ensemble de l’organisme suite une agression sévère, potentiellement mortelle, illustrée par le syndrome de réponse inflammatoire systémique (SIRS).… (more)

▼

L'inflammation systémique est une réaction qui implique l’ensemble de l’organisme suite une agression sévère, potentiellement mortelle, illustrée par le syndrome de réponse inflammatoire systémique (SIRS). De nombreux acteurs cellulaires et moléculaires contribuent au développement de cette cascade inflammatoire parmi lesquels les cellules NK jouent un rôle clé. Malgré l'accumulation de preuves sur l’existence de propriétés spécifiques à chaque organe en réponse à l'inflammation systémique, en termes de cellules NK, on sait peu de choses sur la dynamique compartimentalisée de l’activation des cellules NK pendant un SIRS. En outre, le statut immunitaire des cellules NK après la résolution d’un SIRS est également mal connu. Dans le présent travail, nous avons étudié les réponses des cellules NK provenant de différents organes en utilisant un modèle d’endotoxinémie murine. Nous avons caractérisé la réponse des cellules NK au sein de la rate, du poumon, de la moelle osseuse, de la cavité péritonéale, et dans la circulation. Nous avons trouvé que, malgré une dynamique similaire de la réponse dans les différents organes, les réponses des cellules NK sont compartimentalisées avec des seuils différent et spécifiques. A l’aide de transferts adoptifs, nous avons constaté que la réactivité des cellules NK spécifiques d'organes peut refléter le compartiment d’origine lors des phases initiales de l'inflammation. Cependant, les cellules NK ont la capacité de s’adapter rapidement à leur nouvel environnement et d'ajuster leurs niveaux de réponse à ceux des cellules NK résidentes. Ainsi, cette étude fournit une preuve de concept qui confirme la compartimentalisation de la réponse des cellules NK lors de l'inflammation systémique. Dans une deuxième partie, nous avons analysé le statut des cellules NK à différents moments après une endotoxinémie. Les réponses des cellules NK au sein d’une préparation de cellules de la rate sont fortement supprimées en réponse à une restimulation in vitro, 14 jours après l'endotoxinémie. Cependant, nous avons montré que la réactivité intrinsèque des cellules NK est en fait augmentée après l'injection d’endotoxine, aboutissant à des cellules NK présentant des caractéristiques de cellules NK mémoires. Des expériences de transfert adoptif ont confirmé les propriétés de mémoire des cellules NK après endotoxinémie. Nos résultats accroissent la connaissance concernant le rôle des cellules NK dans un contexte d'inflammation systémique, révélant des réponses compartimentalisés et l’induction d’une mémoire suite à l’endotoxinémie. L'observation selon laquelle les cellules NK développent des propriétés de mémoire après une inflammation systémique dans le contexte d'un environnement suppressif est d’une grande nouveauté et ce phénomène est rapporté pour la première fois.

Systemic inflammation is whole-body reaction to a triggering insult that often results in life threatening illness like systemic inflammatory response syndrome (SIRS). Contributing to the development of this inflammatory cascade are numerous…

Advisors/Committee Members: Cavaillon, Jean-Marc (thesis director).

Subjects/Keywords: Cellules NK; Compartimentalisation; Inflammation systémique; Mémoire innée; NK cells; Compartmentalization; Systemic inflammation; Innate memory; 571.6

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APA (6^th Edition):

Rasid, O. (2016). NK cells and systemic inflammation : compartmentalization and memory responses : Cellules NK et inflammation systémique : compartimentalisation et réponse mémoire. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2016USPCB078

Chicago Manual of Style (16^th Edition):

Rasid, Orhan. “NK cells and systemic inflammation : compartmentalization and memory responses : Cellules NK et inflammation systémique : compartimentalisation et réponse mémoire.” 2016. Doctoral Dissertation, Sorbonne Paris Cité. Accessed June 23, 2018. http://www.theses.fr/2016USPCB078.

MLA Handbook (7^th Edition):

Rasid, Orhan. “NK cells and systemic inflammation : compartmentalization and memory responses : Cellules NK et inflammation systémique : compartimentalisation et réponse mémoire.” 2016. Web. 23 Jun 2018.

Vancouver:

Rasid O. NK cells and systemic inflammation : compartmentalization and memory responses : Cellules NK et inflammation systémique : compartimentalisation et réponse mémoire. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2016. [cited 2018 Jun 23]. Available from: http://www.theses.fr/2016USPCB078.

Council of Science Editors:

Rasid O. NK cells and systemic inflammation : compartmentalization and memory responses : Cellules NK et inflammation systémique : compartimentalisation et réponse mémoire. [Doctoral Dissertation]. Sorbonne Paris Cité; 2016. Available from: http://www.theses.fr/2016USPCB078

17. Basagiannis, Dimitrios. Ο ρόλος της διαμερισματοποίησης του υποδοχέα VEGFR2 στη μεταγωγή του σήματος του VEGF.

Degree: 2013, University of Ioannina; Πανεπιστήμιο Ιωαννίνων

URL: http://hdl.handle.net/10442/hedi/37012

► The vascular system represents one of the primitive systems formed in a developing organism,embracing all tissues and organs. It consists of a complex tubular network… (more)

▼ The vascular system represents one of the primitive systems formed in a developing organism,embracing all tissues and organs. It consists of a complex tubular network of blood vessels comprisedof endothelial cells (ECs), the main cellular unit that orchestrates the formation of the vascular plexus.The homeostasis of endothelium and thus, the transition from quiescence to angiogenesis, and viceversa, is balanced by the response of ECs to angiogenic stimuli. Aberration of this tightly controlledprogram is linked to a variety of disorders, with abnormal angiogenesis being a hallmark of cancerprogression and metastasis. Despite their diversity and complexity, ECs functions are predominantlydirected by the interplay between vascular endothelial growth factor receptors (VEGFRs) and theirligand, VEGF.VEGF is the most potent pro-angiogenic cytokine and a key molecular player in thepathophysiology of vascular system. Binding of VEGF to the extracellular domain of VEGFR2 promotesthe activation of downstream molecules and subsequent initiation of signalling cascades that propagateECs survival, proliferation, migration, differentiation, inflammation, vascular permeability and stem cellmobilization. Given the broad spectrum of action and the pivotal role in the regulation of ECs biology, unraveling of the underlying mechanisms that govern the VEGF-VEGFR2 system is imperative for thecomprehension of vascular pathophysiology and for targeted therapy application.At the cellular level, the response to extracellular stimulants is fine-tuned through thecoordinated regulation of the cellular compartmentalization and the intracellular trafficking of activatedreceptors. Unambiguously, the significance of membrane localization is set by the intrinsic traffickingand signalling of VEGFR2. Here, we employed a comprehensive screening to map all possibleinternalization routes of VEGFR2 and tested their functional significance in vitro and in vivo. Our datarevealed that, in the absence of ligand, VEGFR2 follows solely dynamin-dependent endocytosis, viawhich the receptor escapes from plasma membrane shedding and preserves its functional state untilligand activation. The presence of VEGF introduces macropinocytosis as an alternative internalizationroute for VEGFR2. Experimental approaches that uncouple signalling from trafficking revealed thatVEGFR2 internalization via macropinocytosis is critical for ERK1/2 activation by VEGF, while, despitethe current view, dynamin-mediated endocytosis of VEGFR2 is not essential. However, dynamindependenttrafficking of downstream components of the VEGFR2 cascade, such as PLCγ, is aprerequisite for macropinocytosis-mediated signal propagation, both in vitro and in vivo. These datareveal that propagation of growth factor receptor signalling requires coordinated trafficking of both thereceptor and downstream signalling molecules.

Subjects/Keywords: Υποδοχέας του ενδοθηλιακού αυξητικού παράγοντα; Σηματοδότηση; Διαμερισματοποίηση; Ενδοκυττάρωση; Κυτταρική διακίνηση; VEGFR2; Signalling; Compartmentalization; Endocytosis; Intracellular trafficking

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Basagiannis, D. (2013). Ο ρόλος της διαμερισματοποίησης του υποδοχέα VEGFR2 στη μεταγωγή του σήματος του VEGF. (Thesis). University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Retrieved from http://hdl.handle.net/10442/hedi/37012

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Basagiannis, Dimitrios. “Ο ρόλος της διαμερισματοποίησης του υποδοχέα VEGFR2 στη μεταγωγή του σήματος του VEGF.” 2013. Thesis, University of Ioannina; Πανεπιστήμιο Ιωαννίνων. Accessed June 23, 2018. http://hdl.handle.net/10442/hedi/37012.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Basagiannis, Dimitrios. “Ο ρόλος της διαμερισματοποίησης του υποδοχέα VEGFR2 στη μεταγωγή του σήματος του VEGF.” 2013. Web. 23 Jun 2018.

Vancouver:

Basagiannis D. Ο ρόλος της διαμερισματοποίησης του υποδοχέα VEGFR2 στη μεταγωγή του σήματος του VEGF. [Internet] [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2013. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/10442/hedi/37012.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Basagiannis D. Ο ρόλος της διαμερισματοποίησης του υποδοχέα VEGFR2 στη μεταγωγή του σήματος του VEGF. [Thesis]. University of Ioannina; Πανεπιστήμιο Ιωαννίνων; 2013. Available from: http://hdl.handle.net/10442/hedi/37012

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Mitchell, Michele D. The Color Line and Georgia History Textbooks: A Content Analysis.

Degree: MA, Sociology, 2013, Georgia State University

URL: https://scholarworks.gsu.edu/sociology_theses/38

► The purpose of this research is to define racialized textbook bias, conduct a content analysis of Georgia history textbooks, and answer the following question:… (more)

Subjects/Keywords: Racialized; Textbook; Bias; Omission; Compartmentalization; Marginalization

…operationalized as the compartmentalization, marginalization, and omission of information regarding… …Georgia’s public schools and divulge any marginalization, compartmentalization, and omission of… …marginalization, compartmentalization, and omission of African-Americans in Georgia history textbooks… …students in Georgia’s public schools. The marginalization, compartmentalization, and omission of… …Through compartmentalization students are told that history of Africans and African-Americans is…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mitchell, M. D. (2013). The Color Line and Georgia History Textbooks: A Content Analysis. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/sociology_theses/38

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mitchell, Michele D. “The Color Line and Georgia History Textbooks: A Content Analysis.” 2013. Thesis, Georgia State University. Accessed June 23, 2018. https://scholarworks.gsu.edu/sociology_theses/38.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mitchell, Michele D. “The Color Line and Georgia History Textbooks: A Content Analysis.” 2013. Web. 23 Jun 2018.

Vancouver:

Mitchell MD. The Color Line and Georgia History Textbooks: A Content Analysis. [Internet] [Thesis]. Georgia State University; 2013. [cited 2018 Jun 23]. Available from: https://scholarworks.gsu.edu/sociology_theses/38.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mitchell MD. The Color Line and Georgia History Textbooks: A Content Analysis. [Thesis]. Georgia State University; 2013. Available from: https://scholarworks.gsu.edu/sociology_theses/38

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brigham Young University

19. Law, Yuet Ching. Specific Compartmentalization of IgA ASCs in Mouse Salivary Glands via Differential Expression of Chemokines and Chemokine Receptors.

Degree: MS, 2008, Brigham Young University

URL: https://scholarsarchive.byu.edu/etd/1952

► The mucosal system, which forms a barrier between internal organ systems and the external environment, is frequently exposed to pathogenic microorganisms. Immunoglobulin A (IgA) antibody… (more)

▼ The mucosal system, which forms a barrier between internal organ systems and the external environment, is frequently exposed to pathogenic microorganisms. Immunoglobulin A (IgA) antibody secreting cells (ASCs) localize in the lamina propria, and produce IgA antibodies which help protect mucosal tissues. The concept of a common mucosal immune system in which IgA ASCs have the ability to populate any mucosal site has been proposed (1, 2). However, recent research has suggested that IgA ASCs primed in different mucosal sites might possess different sets of chemokine receptors, and therefore migrate specifically to particular mucosal locations (3). In this study, the specific compartmentalization of IgA ASCs in two mouse salivary glands: sublingual gland (SLG), and submandibular gland (SMG) was studied. It was observed that SLG had 12 times more IgA ASCs per gram of gland than that of SMG (p<0.01). This suggested that IgA ASCs migrated to the two salivary glands with different efficiencies. Since the migration of lymphocytes is mediated by interactions between tissue specific chemokines and chemokine receptors, I hypothesized that the specific compartmentalization of IgA ASCs in the SLG and SMG was mediated by the differential expression of IgA ASC attracting chemokines. Quantitative PCR was used and showed that SLG expressed high levels of CCL28 and its receptor CCR10, which correlated to the distribution of IgA ASCs in the two salivary glands. In agreement with QPCR data, reduced levels of IgA ASCs were found in the SLG of CCR10 deficient mice when compared to wild type (WT) mice. Adoptive transfer of CCR10 deficient mice with WT spleen cells reconstituted the WT phenotype. It was therefore concluded that the interaction between CCL28 and CCR10 play an important role in mediating the migration of IgA ASCs into SLG. These results suggested that the accumulation of IgA ASC to distinct salivary glands is a highly selective process. These data also suggested that homing within mucosal sites is not common but rather a highly regulated process with specific subsets of cells homing to different tissues within the mucosal immune system.

Subjects/Keywords: mucosal system; IgA ASCs; mouse salivary glands; chemokines; CCL28; CCR10; receptors; IgA; compartmentalization; common mucosal immune system; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Law, Y. C. (2008). Specific Compartmentalization of IgA ASCs in Mouse Salivary Glands via Differential Expression of Chemokines and Chemokine Receptors. (Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/etd/1952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Law, Yuet Ching. “Specific Compartmentalization of IgA ASCs in Mouse Salivary Glands via Differential Expression of Chemokines and Chemokine Receptors.” 2008. Thesis, Brigham Young University. Accessed June 23, 2018. https://scholarsarchive.byu.edu/etd/1952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Law, Yuet Ching. “Specific Compartmentalization of IgA ASCs in Mouse Salivary Glands via Differential Expression of Chemokines and Chemokine Receptors.” 2008. Web. 23 Jun 2018.

Vancouver:

Law YC. Specific Compartmentalization of IgA ASCs in Mouse Salivary Glands via Differential Expression of Chemokines and Chemokine Receptors. [Internet] [Thesis]. Brigham Young University; 2008. [cited 2018 Jun 23]. Available from: https://scholarsarchive.byu.edu/etd/1952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Law YC. Specific Compartmentalization of IgA ASCs in Mouse Salivary Glands via Differential Expression of Chemokines and Chemokine Receptors. [Thesis]. Brigham Young University; 2008. Available from: https://scholarsarchive.byu.edu/etd/1952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Matochko, Wadim L. In vitro compartmentalization and deep sequencing enable discovery of functional cell-binding ligands by phage display.

Degree: PhD, Department of Chemistry, 2015, University of Alberta

URL: https://era.library.ualberta.ca/files/cb5644r57s

► Phage display is a technique that accelerated the discovery of peptide and protein-based ligands to numerous targets in academia and industry. Many FDA-approved antibodies and… (more)

▼ Phage display is a technique that accelerated the discovery of peptide and protein-based ligands to numerous targets in academia and industry. Many FDA-approved antibodies and peptides on the market have originated from phage display experiments. However, one of the main drawbacks to this technique is that there are two independent steps in the selection process, an enrichment step for ligands with binding affinity towards a target and an amplification step to amplify the enriched phage clones. The amplification step introduces a bias towards enriching phage clones with a different phenotype based on growth rate. The implication of this bias is that target-binding ligands are enriched from a small subset, with low diversity, of the phage library that contains fast growing phage clones. Furthermore, selection from the fast growing phage population gives rise to a large number of target unrelated ligands. This undesired amplification bias is especially detrimental when selecting for ligands against multi-site binding target, such as cell or tissues. In this thesis, we examine whether the collapse of diversity can be prevented in phage libraries by amplifying these libraries in emulsions. We show that preventing the diversity collapse, we can identify more ligands than the standard selection method and speed up the discovery of ligands to targets with multiple binding sites. This thesis first describes the development of the emulsion amplification technique. We describe the manufacturing of the microfluidic devices and synthesis of the perfluoro-surfactant needed to maintain stable emulsions throughout the amplification process. To analyze the amplification process, we develop a method for deep-sequencing of phage display libraries using Illumina and Ion Torrent platforms, as well as MATLAB scripts, to analyze deep-sequencing data. We applied deep sequencing to examine how diversity of peptides in phage display libraries changes as a result of amplification of libraries in bacteria. Using a Ph.D.-12 library as our model library, we observed that amplification enriches ~150 clones, which dominate ~20% of the library. Deep sequencing, for the first time, characterized the collapse of diversity in phage libraries. We extend the use of next-generation sequencing to characterize the Ph.D. 7 library. Using Illumina and Ion Torrent sequencing and multiple biological replicates of amplification of the Ph.D.-7 library, we identified a focused population of 770 sequences that grow quickly, we term these sequences ‘parasites’. In all, 197 sequences from this population have been identified in literature reports that used Ph.D.-7 library. Many of these enriched sequences have confirmed function (e.g., target binding capacity). The bias in the literature, thus, can be viewed as a selection with two different selection pressures: i) target-binding selection, and ii) amplification-induced selection. Enrichment of parasitic sequences could be minimized if amplification bias is removed. Here, we demonstrate that emulsion amplification…

Subjects/Keywords: Phage display; Deep-sequencing; In vitro compartmentalization

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matochko, W. L. (2015). In vitro compartmentalization and deep sequencing enable discovery of functional cell-binding ligands by phage display. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cb5644r57s

Chicago Manual of Style (16^th Edition):

Matochko, Wadim L. “In vitro compartmentalization and deep sequencing enable discovery of functional cell-binding ligands by phage display.” 2015. Doctoral Dissertation, University of Alberta. Accessed June 23, 2018. https://era.library.ualberta.ca/files/cb5644r57s.

MLA Handbook (7^th Edition):

Matochko, Wadim L. “In vitro compartmentalization and deep sequencing enable discovery of functional cell-binding ligands by phage display.” 2015. Web. 23 Jun 2018.

Vancouver:

Matochko WL. In vitro compartmentalization and deep sequencing enable discovery of functional cell-binding ligands by phage display. [Internet] [Doctoral dissertation]. University of Alberta; 2015. [cited 2018 Jun 23]. Available from: https://era.library.ualberta.ca/files/cb5644r57s.

Council of Science Editors:

Matochko WL. In vitro compartmentalization and deep sequencing enable discovery of functional cell-binding ligands by phage display. [Doctoral Dissertation]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/cb5644r57s

Université Paris-Sud – Paris XI

21. Banach-Latapy, Agata. Monitoring dynamic changes of glutathione redox state in subcellular compartments of human cells : a novel approach based on rxYFP biosensors : Etude des changements dynamiques des états Redox de glutathion dans différents compartiments cellulaires de cellules humaines : Nouvelle approche expérimentale basée sur des biosensuers rxYFP.

Degree: Docteur es, Biologie, 2013, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2013PA112346

►

La biologie des réactions redox est particulièrement difficile à étudier de par la compartimentation spatiale mais aussi cinétique des différents systèmes redox cellulaires. Les biosenseurs… (more)

▼

La biologie des réactions redox est particulièrement difficile à étudier de par la compartimentation spatiale mais aussi cinétique des différents systèmes redox cellulaires. Les biosenseurs codés génétiquement, incluant la «redox-sensitive Yellow Fluorescent Protein» (rxYFP) sont une manière de contourner les limitations des méthodes conventionnelles de mesure du couple glutathion/glutathion disulfure (GSH/GSSG). Cette étude présente l’utilisation des biosenseurs rxYFP pour analyser les états redox dans des différents compartiments cellulaires, et leur dynamique en réponse au stress dans les cellules humaines. La rxYFP exprimée soit dans le cytosol, le noyau ou la matrice mitochondriale de cellules HeLa s’est révélée sensible aux changements de l’état redox intracellulaire provoqué par des traitements aussi bien réducteurs qu’oxydants. La rxYFP est capable de détecter des différences de l’état redox, entre les compartiments, mais aussi entre différentes lignées cellulaires. Les senseurs exprimés dans des kératinocytes humain de l’épiderme HEK001 ont réagi au stress induit par les UVA, de façon dose-dépendante, mais pas au stress induit par les UVB. De plus, ces senseurs ont pu détecter les changements redox induits par des faibles doses (30 µM) ainsi que par des doses modérées (100 µM) de peroxyde d’hydrogène (H2O2), de façon dynamique et spécifique des compartiments cellulaires. La rxYFP exprimé dans la matrice mitochondriale a montré une vitesse d’oxydation plus élevée que les senseurs rxYFP exprimés dans le cytosol ou le noyau, ce qui est attribuable à un pH local plus basique. De plus, la déplétion en GSH provoquée par un traitement au buthionine sulphoximine (BSO) a affecté spécifiquement le potentiel redox mitochondrial mais pas cytosolique ni nucléaire. Ces observations soutiennent l’idée que l’état redox du GSH mitochondrial est maintenu et régulé de façon indépendante par rapport à celui du cytosol ou du noyau. Nous avons également montré que dans les cellules humaines, les sondes rxYFP réagissent de façon prédominante avec le GSH/GSSG, puisque la déplétion en GSH ralentit la vitesse d’oxydation de la rxYFP en réponse à un traitement par H2O2. De plus, grâce à l’utilisation des sondes rxYFP et à l’analyse de l’état redox des antioxydants cellulaires, nous démontrons que l’oxydation des thiols se produit après l’activation des caspases au cours de l’apoptose induite par TRAIL. L’ensemble de nos données montrent la robustesse des senseurs rxYFP pour la mesure des changements d’état redox dans les cellules humaines. En complément d’autres senseurs redox ainsi que des méthodes conventionnelles de mesure des états redox, les senseurs rxYFP ciblés aux différents compartiments cellulaires sont un nouvel outil pour étudier l’homéostasie redox dans les cellules de mammifères, et permettent l’étude de l’état redox du glutathion et de la dynamique des changements redox avec une grande précision.

The kinetic and spatial separation of redox systems renders redox biology studies a particularly challenging field.…

Advisors/Committee Members: Huang, Meng-Er (thesis director).

Subjects/Keywords: Environnement redox; Biosenseur sensible à l’état redox; Glutathione; Séparation spatiale de réactions redox; Redox environment; , redox-sensitive biosensor; Glutathion; Redox compartmentalization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Banach-Latapy, A. (2013). Monitoring dynamic changes of glutathione redox state in subcellular compartments of human cells : a novel approach based on rxYFP biosensors : Etude des changements dynamiques des états Redox de glutathion dans différents compartiments cellulaires de cellules humaines : Nouvelle approche expérimentale basée sur des biosensuers rxYFP. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2013PA112346

Chicago Manual of Style (16^th Edition):

Banach-Latapy, Agata. “Monitoring dynamic changes of glutathione redox state in subcellular compartments of human cells : a novel approach based on rxYFP biosensors : Etude des changements dynamiques des états Redox de glutathion dans différents compartiments cellulaires de cellules humaines : Nouvelle approche expérimentale basée sur des biosensuers rxYFP.” 2013. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed June 23, 2018. http://www.theses.fr/2013PA112346.

MLA Handbook (7^th Edition):

Banach-Latapy, Agata. “Monitoring dynamic changes of glutathione redox state in subcellular compartments of human cells : a novel approach based on rxYFP biosensors : Etude des changements dynamiques des états Redox de glutathion dans différents compartiments cellulaires de cellules humaines : Nouvelle approche expérimentale basée sur des biosensuers rxYFP.” 2013. Web. 23 Jun 2018.

Vancouver:

Banach-Latapy A. Monitoring dynamic changes of glutathione redox state in subcellular compartments of human cells : a novel approach based on rxYFP biosensors : Etude des changements dynamiques des états Redox de glutathion dans différents compartiments cellulaires de cellules humaines : Nouvelle approche expérimentale basée sur des biosensuers rxYFP. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2013. [cited 2018 Jun 23]. Available from: http://www.theses.fr/2013PA112346.

Council of Science Editors:

Banach-Latapy A. Monitoring dynamic changes of glutathione redox state in subcellular compartments of human cells : a novel approach based on rxYFP biosensors : Etude des changements dynamiques des états Redox de glutathion dans différents compartiments cellulaires de cellules humaines : Nouvelle approche expérimentale basée sur des biosensuers rxYFP. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2013. Available from: http://www.theses.fr/2013PA112346

22. Machaieie , João Simião. Aparato para medição de forças e momentos de navios soçobrados.

Degree: 2015, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/12357

►

Com a presente dissertação pretende-se desenvolver um aparato que permita efetuar medições, o mais precisas possível, de forças e momentos para os navios que se… (more)

▼

Com a presente dissertação pretende-se desenvolver um aparato que permita efetuar medições, o mais precisas possível, de forças e momentos para os navios que se encontrem soçobrados. Assim este aparato será projetado para ser simulado no Tanque do Laboratório de Arquitetura Naval, para podermos ter condições que ajudem a estudar os diversos modos de calcular forças e momentos de um navio soçobrado. Esta dissertação divide-se em duas partes, uma teórica e outra prática. Numa primeira parte, na seção do enquadramento teórico, foram abordados conceitos que serviram de base, para o estudo do comportamento da plataforma durante o processo de alagamento, com base em princípios da Arquitetura Naval, nomeadamente da Estabilidade. Com isto, foram realizados cálculos em Excel por forma a conseguir obter os gráficos de carenas direitas e inclinadas. Depois de ter obtido o gráfico de carenas inclinadas, foi mais acessível chegar ao cálculo dos momentos. Com esta base, a parte teórica fica concluída restando apenas a parte prática que irá ser comparada com esta e de seguida conseguiremos tirar algumas conclusões da viabilidade do aparato. Para realizarmos a parte prática, foi necessário fazer algumas alterações ao batelão, como poderemos ver nos capítulos seguintes. Com a ajuda da oficina da EN e da SA, foi possível contruir o aparato. Após construído o aparato, calcular-se-á os respetivos momentos e forças necessários para fazer com que o navio retome a sua posição inicial, tendo em conta as caraterísticas do tanque e adotando determinados pressupostos. Depois de efetuadas a provas no tanque e calibrado os componentes conclui que o sistema funcionava como pretendido inicialmente, pois devido aos pressupostos assumidos, o erro que resultou até foi relativamente baixo como irei demonstrar ao longo da dissertação.

The aim of the present dissertation is to develop a device which allows to measure accurately the forces and moments of shipwrecked vessels. Thus, this device will be designed to be simulated in the tank of the Naval Architecture Laboratory so that there will be the right conditions to help us study the different ways to calculate the forces and moments of shipwrecked vessels. For it is the only place in EN which has conditions that could help in studying the calculations of forces and moments of a capsized ship. This dissertation is divided in two parts- a theoretical and a practical one. In the first part, the theoretical framework section, there’s an approach to the concepts that were the support for the study of the performance of the platform during the overflowing process, taking into account the principles of Naval Architecture, namely the principle of stability. With these fundamentals, calculations were performed in Excel have been done in order to obtain graphs of cross curves and hydrostatic curves. After having obtained the cross curves graphic it was easier to calculate the moments. On this basis, the theoretical part is concluded. The practical part will be compared with the theoretical one in…

Advisors/Committee Members: Rentróia, José Pita Rodrigues.

Subjects/Keywords: Compartimentação, estabilidade, centro de gravidade, metacentro, momento endireitante, movimentação de pesos. 4; Compartmentalization, stability, buoyancy, metacenter, straighten moment, movements of weights.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Machaieie , J. S. (2015). Aparato para medição de forças e momentos de navios soçobrados. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/12357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Machaieie , João Simião. “Aparato para medição de forças e momentos de navios soçobrados.” 2015. Thesis, RCAAP. Accessed June 23, 2018. https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/12357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Machaieie , João Simião. “Aparato para medição de forças e momentos de navios soçobrados.” 2015. Web. 23 Jun 2018.

Vancouver:

Machaieie JS. Aparato para medição de forças e momentos de navios soçobrados. [Internet] [Thesis]. RCAAP; 2015. [cited 2018 Jun 23]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/12357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Machaieie JS. Aparato para medição de forças e momentos de navios soçobrados. [Thesis]. RCAAP; 2015. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/12357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Foureau, Emilien. Elucidation de la voie de biosynthèse des alcaloïdes de Catharanthus roseus et ingénierie métabolique dans la levure : Elucidation of the Catharanthus roseus alkaloid pathway and metabolic engineering in yeast.

Degree: Docteur es, Sciences de la Vie et de la Santé, 2016, Université François-Rabelais de Tours

URL: http://www.theses.fr/2016TOUR3805

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Catharanthus roseus est une plante médicinale produisant divers types d’alcaloïdes indoliques monoterpéniques (AIM) d’intérêt en santé humaine. Ainsi, les AIM dimères comme la vinblastine et… (more)

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Catharanthus roseus est une plante médicinale produisant divers types d’alcaloïdes indoliques monoterpéniques (AIM) d’intérêt en santé humaine. Ainsi, les AIM dimères comme la vinblastine et la vincristine sont utilisés en chimiothérapie anticancéreuse et les alcaloïdes monomères de type hétéroyohimbine présentent diverses activités pharmacologiques. La fabrication de ces molécules dans la plante est fort complexe. Elle requiert un haut niveau de compartimentation tissulaire et subcellulaire et met en jeu plus d’une trentaine d’étapes enzymatiques, dont certaines sont encore très mal connues. Dans ce contexte, l’objectif de la thèse a consisté à élucider plusieurs étapes enzymatiques de la voie de biosynthèse des AIM. Nos travaux ont permis de caractériser de nouvelles isoformes enzymatiques de la famille des cytochromes P450 ainsi que les réductases qui leur sont associées. Ils ont abouti à l’identification de nouvelles déshydrogénases et mis en évidence, in planta, leurs interactions avec la strictosidine synthase suggérant une biosynthèse orientée vers les divers alcaloïdes de type hétéroyohimbine. Enfin, en ayant recours à l’ingénierie métabolique, un segment de la voie de biosynthèse a été transféré dans la levure Saccharomyces cerevisiae, lui conférant la capacité de bio-transformer la tabersonine en vindoline, l’un des deux précurseurs finaux des alcaloïdes dimères.

Catharanthus roseus is a medicinal plant producing various types of monoterpene indole alkaloids (MIA) with a great interest in human health. Dimeric alkaloids such as vinblastine and vincristine are used in cancer chemotherapy and monomeric heteroyohimbine alkaloids exhibit various pharmacological activities. The production of these molecules in the plant is very complex. It requires a high level of tissular and subcellular compartmentalization and involves more than thirty enzymatic steps, some of which are largely unknown. In this context, the aim of this thesis was to elucidate several enzymatic steps of the MIA biosynthetic pathway. Our work allowed us to characterize new enzyme isoforms of cytochrome P450 and their associated reductases. They also resulted in the identification of new dehydrogenases and highlighted their interactions with the strictosidine synthase suggesting a directed biosynthesis towards various heteroyohimbine type of alkaloids. Finally, engineered yeast containing a segment of the MIA biosynthetic pathway was able to convert tabersonine into vindoline, one of the two final precursors of the dimeric alkaloids.

Advisors/Committee Members: Clastre, Marc (thesis director), Courdavault, Vincent (thesis director).

Subjects/Keywords: Catharanthus roseus; Alcaloïdes indoliques monoterpéniques; Biosynthèse; Compartimentation subcellulaire; Ingénierie métabolique; Catharanthus roseus; Monoterpene indole alkaloid; Biosynthesis; Subcellular compartmentalization; Metabolic engineering

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APA (6^th Edition):

Foureau, E. (2016). Elucidation de la voie de biosynthèse des alcaloïdes de Catharanthus roseus et ingénierie métabolique dans la levure : Elucidation of the Catharanthus roseus alkaloid pathway and metabolic engineering in yeast. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2016TOUR3805

Chicago Manual of Style (16^th Edition):

Foureau, Emilien. “Elucidation de la voie de biosynthèse des alcaloïdes de Catharanthus roseus et ingénierie métabolique dans la levure : Elucidation of the Catharanthus roseus alkaloid pathway and metabolic engineering in yeast.” 2016. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed June 23, 2018. http://www.theses.fr/2016TOUR3805.

MLA Handbook (7^th Edition):

Foureau, Emilien. “Elucidation de la voie de biosynthèse des alcaloïdes de Catharanthus roseus et ingénierie métabolique dans la levure : Elucidation of the Catharanthus roseus alkaloid pathway and metabolic engineering in yeast.” 2016. Web. 23 Jun 2018.

Vancouver:

Foureau E. Elucidation de la voie de biosynthèse des alcaloïdes de Catharanthus roseus et ingénierie métabolique dans la levure : Elucidation of the Catharanthus roseus alkaloid pathway and metabolic engineering in yeast. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2016. [cited 2018 Jun 23]. Available from: http://www.theses.fr/2016TOUR3805.

Council of Science Editors:

Foureau E. Elucidation de la voie de biosynthèse des alcaloïdes de Catharanthus roseus et ingénierie métabolique dans la levure : Elucidation of the Catharanthus roseus alkaloid pathway and metabolic engineering in yeast. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2016. Available from: http://www.theses.fr/2016TOUR3805

24. 木下, 貴明. Elucidation of the mechanism underlying the trafficking specifically to the proximal region of an axon in Drosophila neuron.

Degree: 博士（工学）, 2017, Soka University / 創価大学

URL: http://hdl.handle.net/10911/4966

Subjects/Keywords: Drosophila; T antigen; axonal compartmentalization; ASEM; spectraplakin

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APA (6^th Edition):

木下, �. (2017). Elucidation of the mechanism underlying the trafficking specifically to the proximal region of an axon in Drosophila neuron. (Thesis). Soka University / 創価大学. Retrieved from http://hdl.handle.net/10911/4966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

木下, 貴明. “Elucidation of the mechanism underlying the trafficking specifically to the proximal region of an axon in Drosophila neuron.” 2017. Thesis, Soka University / 創価大学. Accessed June 23, 2018. http://hdl.handle.net/10911/4966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

木下, 貴明. “Elucidation of the mechanism underlying the trafficking specifically to the proximal region of an axon in Drosophila neuron.” 2017. Web. 23 Jun 2018.

Vancouver:

木下 �. Elucidation of the mechanism underlying the trafficking specifically to the proximal region of an axon in Drosophila neuron. [Internet] [Thesis]. Soka University / 創価大学; 2017. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/10911/4966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

木下 �. Elucidation of the mechanism underlying the trafficking specifically to the proximal region of an axon in Drosophila neuron. [Thesis]. Soka University / 創価大学; 2017. Available from: http://hdl.handle.net/10911/4966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Colorado State University

25. Higgins, Jenny. Compartmentalization of membrane proteins by the actin cytoskeleton.

Degree: MS(M.S.), Biomedical Engineering, 2007, Colorado State University

URL: http://hdl.handle.net/10217/81011

► Acting as the point of contact for the outside world, the plasma membrane is crucial for cellular signaling events. Proper organization of membrane components is… (more)

Subjects/Keywords: membrane protein; superresolution; plasma membrane compartmentalization; actin; cytoskeleton; PALM

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APA (6^th Edition):

Higgins, J. (2007). Compartmentalization of membrane proteins by the actin cytoskeleton. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/81011

Chicago Manual of Style (16^th Edition):

Higgins, Jenny. “Compartmentalization of membrane proteins by the actin cytoskeleton.” 2007. Masters Thesis, Colorado State University. Accessed June 23, 2018. http://hdl.handle.net/10217/81011.

MLA Handbook (7^th Edition):

Higgins, Jenny. “Compartmentalization of membrane proteins by the actin cytoskeleton.” 2007. Web. 23 Jun 2018.

Vancouver:

Higgins J. Compartmentalization of membrane proteins by the actin cytoskeleton. [Internet] [Masters thesis]. Colorado State University; 2007. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/10217/81011.

Council of Science Editors:

Higgins J. Compartmentalization of membrane proteins by the actin cytoskeleton. [Masters Thesis]. Colorado State University; 2007. Available from: http://hdl.handle.net/10217/81011

University of Michigan

26. Levitt, Erica Sawyer. Factors Influencing Opioid Receptor Signaling to Adenylyl Cyclase.

Degree: PhD, Pharmacology, 2010, University of Michigan

URL: http://hdl.handle.net/2027.42/77937

► Opioids, such as morphine, signal through G protein-coupled receptors that are linked to adenylyl cyclase (AC)-inhibitory (Gi/o) heterotrimeric GTP-binding proteins. The transduction of signal from… (more)

▼ Opioids, such as morphine, signal through G protein-coupled receptors that are linked to adenylyl cyclase (AC)-inhibitory (Gi/o) heterotrimeric GTP-binding proteins. The transduction of signal from opioid receptor to G protein is dependent on the organization of proteins in the membrane and can be modulated by alterations in membrane lipids. This thesis provides evidence that mu-opioid receptors (MOR) and delta-opioid receptors (DOR) are differentially regulated by cholesterol. I have determined that cholesterol is required to stabilize MOR in a high-affinity conformation that couples to G proteins. Thus, cholesterol depletion attenuated the efficiency of MOR signaling to G proteins or AC. In contrast, DOR signaling was unaffected by removal of membrane cholesterol. Cholesterol depletion also attenuated the ability of MOR agonists to produce cellular adaptations leading to cAMP overshoot. This was true of heterologously expressed MOR in HEK293 cells, and endogenously expressed MOR in human neuroblastoma SH-SY5Y cells. Cholesterol may modulate MOR signaling by a direct interaction with the receptor or by formation of cholesterol-enriched membrane microdomains. Indeed, a portion of cell surface MOR was associated with markers of cholesterol-enriched membrane microdomains; however, these studies do not confirm that this association is responsible for the effect of cholesterol depletion on MOR signaling. In contrast, DOR was not associated with membrane microdomain markers, even after agonist treatment. MOR and DOR are thus likely not compartmentalized together within cholesterol-enriched membrane microdomains. Regardless, MOR and DOR in SH-SY5Y cells were observed to share a common pool of G proteins and AC, which resulted in occlusion of DOR agonist responses in the presence of maximally effective concentrations of MOR agonist. Moreover, this was not limited to MOR and DOR, as all Gi/o-coupled receptors endogenously expressed in SH-SY5Y cells were able to access a shared pool of AC. After chronic administration of MOR agonist, the addition of agonists to DOR, alpha2-adrenergic receptors (alpha2AR) and nociceptin/OFQ peptide receptors (NOPr) reduced the expression of MOR-mediated cAMP overshoot. Given the co-expression of alpha2AR and NOPr on MOR-containing neurons, the ability of agonists to these receptors to reduce MOR-mediated cAMP overshoot has implications in the treatment of opioid withdrawal symptoms. Advisors/Committee Members: Traynor, John R. (committee member), Martens, Jeffrey (committee member), Mosberg, Henry I. (committee member), Sunahara, Roger K. (committee member).

Subjects/Keywords: Opioid Receptors; Adenylyl Cyclase; Membrane Cholesterol; Compartmentalization; GPCR; Lipid Rafts; Pharmacy and Pharmacology; Health Sciences; Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Levitt, E. S. (2010). Factors Influencing Opioid Receptor Signaling to Adenylyl Cyclase. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/77937

Chicago Manual of Style (16^th Edition):

Levitt, Erica Sawyer. “Factors Influencing Opioid Receptor Signaling to Adenylyl Cyclase.” 2010. Doctoral Dissertation, University of Michigan. Accessed June 23, 2018. http://hdl.handle.net/2027.42/77937.

MLA Handbook (7^th Edition):

Levitt, Erica Sawyer. “Factors Influencing Opioid Receptor Signaling to Adenylyl Cyclase.” 2010. Web. 23 Jun 2018.

Vancouver:

Levitt ES. Factors Influencing Opioid Receptor Signaling to Adenylyl Cyclase. [Internet] [Doctoral dissertation]. University of Michigan; 2010. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/2027.42/77937.

Council of Science Editors:

Levitt ES. Factors Influencing Opioid Receptor Signaling to Adenylyl Cyclase. [Doctoral Dissertation]. University of Michigan; 2010. Available from: http://hdl.handle.net/2027.42/77937

University of Michigan

27. Kojima, Taisuke. Development Of Microcompartmentalization Using Macromolecules And Liquid-Liquid Interfaces.

Degree: PhD, Macromolecular Science and Engineering, 2016, University of Michigan

URL: http://hdl.handle.net/2027.42/120885

► Compartmentalization combined with regulated exchange and release of biomaterials is the key biological function. At the subcellular level, organelles sequester enzymes and substrates for storage… (more)

▼ Compartmentalization combined with regulated exchange and release of biomaterials is the key biological function. At the subcellular level, organelles sequester enzymes and substrates for storage while processing and releasing on demand. At the organism level, appropriate cellular compartmentalization gives rise to tissue organization and higher order function. Man-made bioreaction compartmentalization typically relies on micro-reactors constructed with glass, silicon, hard plastic, or amiphiphilic molecular walls. Reactors with such physical barriers, in return for excellent compartmentalization, lack amenability to molecular and cellular release and exchange as seen in the body. This thesis describes the engineering of multi-compartment bioreactors that utilize stable reagent partitioning within immiscible aqueous solutions. The all aqueous compartmentalization scheme, when combined with a micropatterned surface that pins the liquid-liquid interfaces, enable versatile direct printing of arbitrary multi-compartment bioreactor networks. Additionally, the lack of physical barriers at the liquid-liquid interface between the different aqueous compartments allows use of phase-altering chemicals to trigger rapid and selective mixing of materials between compartments in ways not possible with conventional miroreactors. Specific examples demonstrated include a compartmentalized cascade reaction that utilizes glucose and oxygen as nutrients to catalzye the production of different colored dyes within microcompartments and the localization-enhanced degradation of the microcompartments with a polysaccharide-degrading enzyme. In a separate strategy, a shrinking microreactor platform was created using oil dehydration. These shrinking bioreactors were utilized for micro-scale ATPS phase diagram determination and also adopted for micro-scale self-assembly of CdTe nanoparticles. Finally, surface-templated hydrogel micropatterning was used to compartmentalize cancer cells as well as limit diffusion of chemoattractants. This platform revealed the importance of extracellular matrix-mediated capture and localization of chemoattractants in triggering cancer cell migration that lead to a breakdown of cellular compartmentalization, tissue dis-organization and cancer metastasis. In addition to the specific demonstrations and biological insights obtained, the described macromolecular phase-separation microreactor platforms are versatile in configuration possibilities, particularly when combined with microfabrication technologies, and amenable to further explore materials science and biomedical engineering applications. Advisors/Committee Members: Takayama, Shuichi (committee member), Kotov, Nicholas (committee member), Lahann, Joerg (committee member), Robertson, Richard E (committee member), Tuteja, Anish (committee member).

Subjects/Keywords: Compartmentalization; Microreactor; Macromolecule; Liquid-liquid Interface; Biomedical Engineering; Engineering (General); Materials Science and Engineering; Chemistry; Science (General); Engineering; Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kojima, T. (2016). Development Of Microcompartmentalization Using Macromolecules And Liquid-Liquid Interfaces. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/120885

Chicago Manual of Style (16^th Edition):

Kojima, Taisuke. “Development Of Microcompartmentalization Using Macromolecules And Liquid-Liquid Interfaces.” 2016. Doctoral Dissertation, University of Michigan. Accessed June 23, 2018. http://hdl.handle.net/2027.42/120885.

MLA Handbook (7^th Edition):

Kojima, Taisuke. “Development Of Microcompartmentalization Using Macromolecules And Liquid-Liquid Interfaces.” 2016. Web. 23 Jun 2018.

Vancouver:

Kojima T. Development Of Microcompartmentalization Using Macromolecules And Liquid-Liquid Interfaces. [Internet] [Doctoral dissertation]. University of Michigan; 2016. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/2027.42/120885.

Council of Science Editors:

Kojima T. Development Of Microcompartmentalization Using Macromolecules And Liquid-Liquid Interfaces. [Doctoral Dissertation]. University of Michigan; 2016. Available from: http://hdl.handle.net/2027.42/120885

University of Texas – Austin

28. -9135-1620. Sequence stratigraphy and depositional systems in the Upper Cretaceous (Cenomanian) Woodbine Group, Anderson and Cherokee Counties, Texas.

Degree: Geological Sciences, 2016, University of Texas – Austin

URL: http://hdl.handle.net/2152/44420

► The Woodbine Group of the East Texas Basin has attracted considerable interest because of its remaining petroleum resource in the deeper Woodbine pay. Recent estimate… (more)

▼ The Woodbine Group of the East Texas Basin has attracted considerable interest because of its remaining petroleum resource in the deeper Woodbine pay. Recent estimate of the remaining petroleum resources in the East Texas field is approximately 1.58 billion stock tank barrels (BSTB) (Wang et al., 2008). However, expected ultimate recovery is limited by reservoir compartmentalization controlled by a complex stratigraphic framework. The purpose of this study is to define depositional systems and construct the stratigraphic framework of the Woodbine Group in Anderson and Cherokee Counties to provide the geologic context for characterizing remaining reserves. This study integrates core data and log data from closely spaced wireline logs (~1000 wells), using a chronostratigraphic method, to define sequence stratigraphic units. The stratigraphic framework of the Woodbine succession in the study area is composed of a maximum of 14 cycles in the basin axis, decreasing to a minimum of 3 cycles eastward to the Sabine Uplift and a minimum of 6 cycles westward to the out crop belt. The Woodbine succession is overlain by impermeable deposits of the Eagle Ford Shale and the Austin chalk as hydrocarbon seals. The complexity and heterogeneity of sandstone bodies in the Woodbine Group are largely controlled by depositional origin. Woodbine highstand and lowstand sequences display great variations in the depositional systems. The highstand deposits are composed mostly of fluvial dominated delta deposits that consist of distributary-channel, crevasse-splay, and delta-front deposits. Gamma-ray and spontaneous potential responses for these highstand deposits are upward-coarsening and serrate. In contrast, Woodbine lowstand deposits are mainly composed of coarse-grained sandstones of incised valley fills, reflecting relative base-level fall. These lowstand deposits, truncate older highstand deposits and are inferred from planar-based and blocky serrate log responses. Furthermore, highstand and lowstand deposits are overlain by transgressive deposits. These transgressive deposits are characterized by upward-fining log response, reflecting relative base-level rise. Correlation of sequence stratigraphic surfaces, sandstone-body stacking patterns and reservoir complexity inferred from gross-sandstone maps can lead to new exploration targets in the Woodbine Group in the southern part of the East Texas Basin. Advisors/Committee Members: Fisher, W. L. (William Lawrence), 1932- (advisor), Ambrose, William A. (advisor), Mohrig, David (committee member).

Subjects/Keywords: Petroleum reservoirs; Stratigraphic traps; Woodbine Group; Texas; Reservoir compartmentalization; Sandstone; Depositional environments; Lithology; Facies distribution; Anderson County; Cherokee County

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

-9135-1620. (2016). Sequence stratigraphy and depositional systems in the Upper Cretaceous (Cenomanian) Woodbine Group, Anderson and Cherokee Counties, Texas. (Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/44420

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

-9135-1620. “Sequence stratigraphy and depositional systems in the Upper Cretaceous (Cenomanian) Woodbine Group, Anderson and Cherokee Counties, Texas.” 2016. Thesis, University of Texas – Austin. Accessed June 23, 2018. http://hdl.handle.net/2152/44420.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

-9135-1620. “Sequence stratigraphy and depositional systems in the Upper Cretaceous (Cenomanian) Woodbine Group, Anderson and Cherokee Counties, Texas.” 2016. Web. 23 Jun 2018.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-9135-1620. Sequence stratigraphy and depositional systems in the Upper Cretaceous (Cenomanian) Woodbine Group, Anderson and Cherokee Counties, Texas. [Internet] [Thesis]. University of Texas – Austin; 2016. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/2152/44420.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

-9135-1620. Sequence stratigraphy and depositional systems in the Upper Cretaceous (Cenomanian) Woodbine Group, Anderson and Cherokee Counties, Texas. [Thesis]. University of Texas – Austin; 2016. Available from: http://hdl.handle.net/2152/44420

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

29. Faure-Della Corte, Muriel. Cytomégalovirus Humain : variabilité, Recombinaison et Protéine pUL40 : Human Cytomegalovirus : variability, Recombination and Protein pUL40.

Degree: Docteur es, Sciences, technologie, santé. Microbiologie, 2010, Université de Bordeaux Segalen

URL: http://www.theses.fr/2010BOR21772

►

Le cytomégalovirus humain (CMV) appartient à la sous-famille des Betaherpès virus. L’homme est son seul réservoir et il infecte 40 à 90% de la population… (more)

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Le cytomégalovirus humain (CMV) appartient à la sous-famille des Betaherpès virus. L’homme est son seul réservoir et il infecte 40 à 90% de la population mondiale. Ce virus, rarement dangereux pour le sujet immunocompétent, représente une réelle menace chez le patient immunodéprimé comme le transplanté d’organe. Après la primo-infection, le CMV diffuse dans tout l’organisme et alterne des phases de latence et de réactivation. Il consacre 20% de son génome à diverses stratégies d’échappement immunitaire.Les objectifs de notre travail sont de décrire dans une première partie 1- la variabilité de six gènes, codant quatre protéines immunomodulatrices (UL18, UL40, UL111a et US3) et deux protéines immunodominantes (IE1 et pp65), dans des souches de CMV directement séquencées à partir de sang total ou du LBA de patients infectés et immunodéprimés, 2- le mécanisme conduisant à cette variabilité 3- la répartition des souches virales dans le sang total et le LBA (compartimentation).La deuxième partie est consacrée à l’expression de la protéine immunomodulatrice pUL40.Nos travaux ont permis de confirmer l’existence d’un polymorphisme notable, supérieur à ce qui est déjà décrit. Des conséquences fonctionnelles pourraient en découler dans les protéines correspondantes. L’étude in silico de la variabilité du CMV met en lumière le rôle clé de la recombinaison comme mécanisme évolutif majeur. Nous n’avons pas mis en évidence de compartimentation des souches virales dans les échantillons analysés.Nos résultats préliminaires indiquent la faisabilité de la sur-expression de la protéine pUL40, permettant ultérieurement de mieux comprendre ses fonctions.

Human Cytomegalovirus (CMV) belongs to the Betaherpesviruses sub-family. Human beings are its sole reservoir and it infects 40 to 90% of the world population. This virus is rarely dangerous for the immunocompetent host, but represents a problematic opportunistic agent in immunocompromised patients, such as transplant recipients. After primary infection, CMV spreads widely in the organism and alternates latency and reactivation phases. CMV dedicates 20% of its genome to various immune escape strategies.Our aims were to describe, in a first part, 1- the variability of six CMV genes, which encode 4 immunomodulatory proteins (UL18, UL40, UL111a and US3) and two immunodominant proteins (IE1 and pp65), after direct amplification and sequencing from whole blood and bronchoalveolar lavages (BAL) taken from infected immune compromised patients, 2- the mechanism responsible for this variability 3- the distribution of CMV strains in whole blood and BAL (compartmentalization).In a second part, we attempted pUL40 expression.Our results confirmed the existence of a noticeable polymorphism, superior to what was previously described. Functional consequences could arise for the corresponding proteins. Our in silico study of CMV variation indicated a key role for recombination as a major evolutionary mechanism. We have observed little CMV compartmentalization among the investigated samples. Our…

Advisors/Committee Members: Lafon, Marie-Edith (thesis director).

Subjects/Keywords: CMV; Variabilité; Recombinaison; Souches cliniques de CMV; Compartimentation; Outils bioinformatiques; Expression pUL40; CMV; Variability; Recombination; CMV clinical strains; Compartmentalization; Sequence analysis; PUL40 expression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Faure-Della Corte, M. (2010). Cytomégalovirus Humain : variabilité, Recombinaison et Protéine pUL40 : Human Cytomegalovirus : variability, Recombination and Protein pUL40. (Doctoral Dissertation). Université de Bordeaux Segalen. Retrieved from http://www.theses.fr/2010BOR21772

Chicago Manual of Style (16^th Edition):

Faure-Della Corte, Muriel. “Cytomégalovirus Humain : variabilité, Recombinaison et Protéine pUL40 : Human Cytomegalovirus : variability, Recombination and Protein pUL40.” 2010. Doctoral Dissertation, Université de Bordeaux Segalen. Accessed June 23, 2018. http://www.theses.fr/2010BOR21772.

MLA Handbook (7^th Edition):

Faure-Della Corte, Muriel. “Cytomégalovirus Humain : variabilité, Recombinaison et Protéine pUL40 : Human Cytomegalovirus : variability, Recombination and Protein pUL40.” 2010. Web. 23 Jun 2018.

Vancouver:

Faure-Della Corte M. Cytomégalovirus Humain : variabilité, Recombinaison et Protéine pUL40 : Human Cytomegalovirus : variability, Recombination and Protein pUL40. [Internet] [Doctoral dissertation]. Université de Bordeaux Segalen; 2010. [cited 2018 Jun 23]. Available from: http://www.theses.fr/2010BOR21772.

Council of Science Editors:

Faure-Della Corte M. Cytomégalovirus Humain : variabilité, Recombinaison et Protéine pUL40 : Human Cytomegalovirus : variability, Recombination and Protein pUL40. [Doctoral Dissertation]. Université de Bordeaux Segalen; 2010. Available from: http://www.theses.fr/2010BOR21772

30. Παπαδάκης, Βασίλειος. Πολυανθεκτική pseudomonas aeruginosa: μηχανισμός πειραματικής σήψης: επίδραση στη συσταλτικότητα του μυοκαρδίου.

Degree: 2013, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/29755

►

Recent evidence suggests for a link between excess lipid peroxidation andspecific organ failures in sepsis. No study has ever been performed in sepsisby multidrug-resistant (MDR)… (more)

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Recent evidence suggests for a link between excess lipid peroxidation andspecific organ failures in sepsis. No study has ever been performed in sepsisby multidrug-resistant (MDR) Gram-negative bacteria. Lethal sepsis wasinduced in rats by the intraperitoneal injection of one MDR isolate ofPseudomonas aeruginosa. Produced malondialdehyde (MDA) was measuredin tissues five hours after bacterial challenge with the thiobarbiturate assayfollowed by HPLC analysis. MDA of septic rats was greater in the liver, spleenand aortic wall and it was lower in the right kidney compared to shamoperatedanimals. Intravenous administration of low doses of hydrocortisone30 minutes after bacterial challenge reduced significantly and specificallyaorta MDA levels. Animal findings suggest for a compartmentalization of lipidperoxidation in systemic infections by MDR Gram-negative bacteria. Theyalso indicate a selective mechanism of action of low dose of hydrocortisoneexplaining, at least in part, the clinical efficacy of this therapeutic regimen forthe achievement of hemodynamic stability in the event of septic shock.

Πρόσφατα στοιχεία υποδεικνύουν διασύνδεση μεταξύ υπερβολικήςυπεροξείδωσης των λιπιδίων και ανεπάρκειες ορισμένων οργάνων πουοφείλονται στη σήψη. Καμία μελέτη όμως δεν έχει ποτέ πραγματοποιηθεί στησήψη που οφείλεται από πολυανθεκτικά (MDR) Gram-αρνητικά βακτήρια. Γιανα μελετήσουμε αυτό, προκαλέσαμε σήψη σε αρουραίους μέσωενδοπεριτοναϊκής έγχυσης ενός MDR μικροβίου της Pseudomonasaeruginosa. Η εκλυόμενη μάλονοδιαλδεϋδη (MDA) μετρήθηκε στους ιστούςμετά ομογενοποίηση και ανάλυση από σύστημα HPLC. Η παραπάνω μελέτησήψης σε αρουραίους ήταν θανατηφόρα. Η ιστική MDA ήταν μεγαλύτερη στοτοίχωμα του ήπατος, του σπλήνα και της αορτής, αλλά όχι στην καρδιά καιστους πνεύμονες σε σύγκριση με εικονικά χειρουργημένα ζώα. Επίσης ήτανμειωμενα στους νεφρούς συγκριτικά με τους υγιείς μάρτυρες. Η χορήγησηχαμηλής δόσης υδροκορτιζόνης 30 λεπτά μετά τη βακτηριακή πρόκλησημείωσε σημαντικά τις συγκεντρώσεις της MDA εκλεκτικά στο τοίχωμα τηςαορτής. Τα ευρήματα προτείνουν τη διαμερισματοποίηση της υπεροξείδωσηςτων λιπιδίων σε συστηματικές λοιμώξεις από MDR Gram-αρνητικά βακτήρια.Παράλληλα υποδεικνύουν ένα εκλεκτικό μηχανισμό δράσης της χαμηλήςδόσης υδροκορτιζόνης ερμηνεύοντας, εν μέρει, την κλινικήαποτελεσματικότητά της στην επίτευξη αιμοδυναμικής αστάθειας στο σηπτικόασθενή.

Subjects/Keywords: Υπεροξείδωση λιπιδίων; Σήψη; Ψευδομονάδα; Μαλονοδιαλδεΰδη; Υδροκορτιζόνη ( HC); Διαμερισματοποίηση υπεροξείδωση λιπιδίων; Gram αρνητικά βακτήρια; Αιμοδυναμική αστάθεια; Lipid peroxidation; Sepsis; Pseudomonas aeruginosa; Malondialdehyde; Hydrocortizone; Compartmentalization lipid peroxidation; Gram negative bacteria; Hemodynamic stability

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APA (6^th Edition):

Παπαδάκης, . �. (2013). Πολυανθεκτική pseudomonas aeruginosa: μηχανισμός πειραματικής σήψης: επίδραση στη συσταλτικότητα του μυοκαρδίου. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/29755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Παπαδάκης, Βασίλειος. “Πολυανθεκτική pseudomonas aeruginosa: μηχανισμός πειραματικής σήψης: επίδραση στη συσταλτικότητα του μυοκαρδίου.” 2013. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed June 23, 2018. http://hdl.handle.net/10442/hedi/29755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Παπαδάκης, Βασίλειος. “Πολυανθεκτική pseudomonas aeruginosa: μηχανισμός πειραματικής σήψης: επίδραση στη συσταλτικότητα του μυοκαρδίου.” 2013. Web. 23 Jun 2018.

Vancouver:

Παπαδάκης �. Πολυανθεκτική pseudomonas aeruginosa: μηχανισμός πειραματικής σήψης: επίδραση στη συσταλτικότητα του μυοκαρδίου. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. [cited 2018 Jun 23]. Available from: http://hdl.handle.net/10442/hedi/29755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Παπαδάκης �. Πολυανθεκτική pseudomonas aeruginosa: μηχανισμός πειραματικής σήψης: επίδραση στη συσταλτικότητα του μυοκαρδίου. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. Available from: http://hdl.handle.net/10442/hedi/29755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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