subject:(Co t)

University of Alberta

1. Hockley, Deanna L. Co-stimulator contributions in CD8+ T cell differentiation.

Degree: PhD, Department of Medical Microbiology and Immunology, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/f4752h04p

► The adaptive immune response against intracellular pathogens is largely mediated by CD8+ T lymphocytes. The clonal expansion and expression of cytolytic and immune stimulatory proteins… (more)

▼ The adaptive immune response against intracellular pathogens is largely mediated by CD8+ T lymphocytes. The clonal expansion and expression of cytolytic and immune stimulatory proteins by CD8+ T cells is responsible for their protective immune function. Prior to exhibiting effector activity, CD8+ T cells exist in a naïve state and require three stimulatory signals for their optimal activation including the recognition of antigen, co-stimulator ligand engagement, and the presence of pro-inflammatory cytokines. When these activation requirements are met, CD8+ T cells undergo a well described series of events including clonal expansion, cellular contraction, memory generation, and memory maintenance. The memory CD8+ T cell population generated can survive for the life-time of the host, and provides more rapid and robust protection upon re-infection then their naïve precursors. While some factors that induce this sequence of events have been identified, the role of co-stimulation remains relatively undefined. This is due to the large number of co-stimulator receptors expressed by CD8+ T cells that may be ligated individually and/or in combination, to instruct the development of specific effector and memory CD8+ T cell phenotypes. Using a bead-based ligand presentation system, I investigated the role of co-stimulation in directing naïve CD8+ T cell activation, and the generation of T cell populations with distinct effector and memory fates. I identified ICAM-1 as the stimulatory molecule best able to induce naïve CD8+ T cell proliferation and expression of the effector molecule granzyme B, while co-stimulation through CD28 was required to induce IFN-γ. When provided in combination however, B7.1 and ICAM-1 co-stimulation generated CD8+ T cells which were highly cytolytic and expressed high amounts of IFN-γ. These cells also exhibited enhanced survival once activated, with sustained expression of anti-apoptotic proteins and secretion of high amounts of IL-2, resulting in these cells exhibiting a terminal effector phenotype. While other co-stimulator combinations also enhanced CD8+ T cell survival to some degree, their ability to sustain high expression of IL-2 was limited. This translated into less potent effector responses and preferential memory precursor development based on transcription factor expression.

Subjects/Keywords: Immunology; T cells; Co-stimulation

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APA (6^th Edition):

Hockley, D. L. (2012). Co-stimulator contributions in CD8+ T cell differentiation. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/f4752h04p

Chicago Manual of Style (16^th Edition):

Hockley, Deanna L. “Co-stimulator contributions in CD8+ T cell differentiation.” 2012. Doctoral Dissertation, University of Alberta. Accessed March 06, 2021. https://era.library.ualberta.ca/files/f4752h04p.

MLA Handbook (7^th Edition):

Hockley, Deanna L. “Co-stimulator contributions in CD8+ T cell differentiation.” 2012. Web. 06 Mar 2021.

Vancouver:

Hockley DL. Co-stimulator contributions in CD8+ T cell differentiation. [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2021 Mar 06]. Available from: https://era.library.ualberta.ca/files/f4752h04p.

Council of Science Editors:

Hockley DL. Co-stimulator contributions in CD8+ T cell differentiation. [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/f4752h04p

2. Messal, Nassima. Expression, régulation et caractérisation fonctionnelle des molécules de co-signalisation immunitaire, PD-L2 et CD277 dans l'activation lymphocytaire T : Expression and regulation of PD-L2, B7 family member and functional characterization on T cells.

Degree: Docteur es, Sciences de la vie et de la sante, 2011, Aix-Marseille 2

URL: http://www.theses.fr/2011AIX20654

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Programmed death-1 (PD-1) et ses ligands PD-L1 et PD-L2 appartiennent à la famille B7 : CD28 des molécules de cosignalisation immunitaire. Leur interaction délivre un… (more)

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Programmed death-1 (PD-1) et ses ligands PD-L1 et PD-L2 appartiennent à la famille B7 : CD28 des molécules de cosignalisation immunitaire. Leur interaction délivre un signal inhibiteur à l’activation lymphocytaire. Ces molécules sont impliquées non seulement dans le contrôle de la tolérance, mais aussi dans un mécanisme d’échappement tumoral et viral au système immunitaire. Les deux ligands de PD-1 : PD-L1 et PD-L2 montrent une expression tissulaire assez large, témoignant de leur rôle étendu aux différents stades de la réponse immunitaire. L’expression de PD-L1 est plus large que celle de PD-L2. PD-L1 est exprimé sur les cellules hématopoïétiques et non hématopoïétiques, PDL2 présente une expression assez restreinte aux CPAs professionnelles. L’expression de PD-L1 et PD-L2 est fortement régulée dans plusieurs cas pathologiques et notamment dans les cancers. La régulation de l’expression de PD-L2 qui fait l’objet de notre étude, représente une cible importante dans le domaine de l’immunothérapie. Notre projet porte sur l’analyse de l’expression, la régulation et la fonction de PD-L2 dans les LT, dans différents contextes physiologiques et pathologiques. Nous avons pu démontrer que PD-L2 qui avait été décrit chez la souris pour son expression restreinte aux CDs et aux macrophages, était de façon surprenante exprimé avec son homologue PD-L1 sur les LT après costimulation CD3+CD28. Nous l’avons confirmé ex vivo par activation par Staphylococcal enterotoxin E. Ce résultat montre que PD-L2 est bien induit sur des LT dans des conditions d’activation physiologique ou pathologique. Nous avons pu démontrer aussi une régulation négative de l’expression transcriptionnelle et protéique de PD-L1 et de PDL2 sous l’effet de la CyclosporineA (CsA). Cet effet est indépendant de l’action de l’IL-2. Nos données d’analyses bioinformatiques nous ont permis d’identifier les sites putatifs de fixation de facteurs de transcription au niveau du promoteur du gène pd-l2, qui peuvent être régulés par la CsA. L’analyse de la fonction de PDL2 sur les LT nous a permis de démontrer que la stimulation de PDL2 par des anticorps monoclonaux fabriqués dans le laboratoire inhibait l’activation T. Nos résultats démontrent pour la première fois une fonction de PDL2 et une signalisation « reverse siglaling » dans les LT potentiels. La caractérisation fonctionnelle et l’étude des voies de signalisation de PD-L2 que nous avons déjà étudié dans notre projet, représentent une piste importante à explorer. Cela permettrait de proposer de nouvelles stratégies permettant de lutter contre les mécanismes d’échappement tumoral.

PD-1 (programmed death-1) is a new CD28 families member, that deliver inhibitory signals that regulate the balance between T cell activation, tolerance, and immunopathology. (1) (ref octa).PD-1, is inducibly expressed on T cells. The PD-1 ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) exhibit distinct patterns of expression: PD-L1 is expressed more broadly than is PD-L2. PD-L1 is expressed on resting and up regulated on hematopoietic,…

Advisors/Committee Members: Olive, Daniel (thesis director).

Subjects/Keywords: Co-stimulation; Activation T; Pd-l2; Cd277; Co-signalisation; Co-inhibition immunitaire; CsA; Co-stimulation; Activation; Pd-l2; Cd277; Co-signalisation; Co-inhibition of immunity; CsA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Messal, N. (2011). Expression, régulation et caractérisation fonctionnelle des molécules de co-signalisation immunitaire, PD-L2 et CD277 dans l'activation lymphocytaire T : Expression and regulation of PD-L2, B7 family member and functional characterization on T cells. (Doctoral Dissertation). Aix-Marseille 2. Retrieved from http://www.theses.fr/2011AIX20654

Chicago Manual of Style (16^th Edition):

Messal, Nassima. “Expression, régulation et caractérisation fonctionnelle des molécules de co-signalisation immunitaire, PD-L2 et CD277 dans l'activation lymphocytaire T : Expression and regulation of PD-L2, B7 family member and functional characterization on T cells.” 2011. Doctoral Dissertation, Aix-Marseille 2. Accessed March 06, 2021. http://www.theses.fr/2011AIX20654.

MLA Handbook (7^th Edition):

Messal, Nassima. “Expression, régulation et caractérisation fonctionnelle des molécules de co-signalisation immunitaire, PD-L2 et CD277 dans l'activation lymphocytaire T : Expression and regulation of PD-L2, B7 family member and functional characterization on T cells.” 2011. Web. 06 Mar 2021.

Vancouver:

Messal N. Expression, régulation et caractérisation fonctionnelle des molécules de co-signalisation immunitaire, PD-L2 et CD277 dans l'activation lymphocytaire T : Expression and regulation of PD-L2, B7 family member and functional characterization on T cells. [Internet] [Doctoral dissertation]. Aix-Marseille 2; 2011. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2011AIX20654.

Council of Science Editors:

Messal N. Expression, régulation et caractérisation fonctionnelle des molécules de co-signalisation immunitaire, PD-L2 et CD277 dans l'activation lymphocytaire T : Expression and regulation of PD-L2, B7 family member and functional characterization on T cells. [Doctoral Dissertation]. Aix-Marseille 2; 2011. Available from: http://www.theses.fr/2011AIX20654

3. Ellestad, Kristofor K. Mechanisms underlying lymphopenia-driven autoimmunity in the setting of co-inhibitory molecule deficiency.

Degree: PhD, Department of Medical Microbiology and Immunology, 2016, University of Alberta

URL: https://era.library.ualberta.ca/files/ckk91fk70f

► T lymphocytes (T cells) are powerful directors and effectors of immunity. The system of pseudo-random rearrangements of the T cell receptor (TCR) loci that underlie… (more)

▼ T lymphocytes (T cells) are powerful directors and effectors of immunity. The system of pseudo-random rearrangements of the T cell receptor (TCR) loci that underlie their ability to recognize a vast universe of molecular patterns is at once useful and dangerous, because many T cells develop TCR that can recognize self, and while most developing T cells with high affinity for self are removed from the repertoire during thymic development, the process is not perfect and some dangerous clones escape. These newly generated T cells have only passed through one “filtration” process in the thymus, and therefore peripheral tolerance mechanisms are critical in order to avoid autoimmunity. Homeostasis of T cells in the periphery is tightly regulated by competition for a finite resource pool, including homeostatic cytokines and relevant peptide:MHC (pMHC) complexes with which a T cell can interact and receive at least a “tonic” or greater signal. In conditions of lymphopenia, which can arise as a consequence of viral infections, clinical interventions, and other stimuli, resources are in excess and T cells will undergo a process of lymphopenia-induced proliferation (LIP) to fill their available niche. Importantly, LIP is strongly associated with inflammatory disease. The co-inhibitory receptor programmed death-1 (PD-1) is expressed on T cells where it provides inhibitory signals that help prevent inappropriate T cell activation or keep T cells in an unresponsive state. PD-1-/- mice are predisposed to infrequent and mild autoimmunity. In contrast, reconstitution of the lymphoid compartment of lymphopenic adult Rag-/- mice with PD-1-/- hematopoietic stem cells (HSC) gives rise to a rapid and severe systemic autoimmune disease shortly after the first newly generated T cells emerge from the thymus. Thus a “three strikes” combination of newly generated T cells, deficiency of PD-1, and LIP synergizes to promote autoimmunity. Our goal herein was to explore the mechanisms and cells underlying the disease in this model. One of PD-1’s ligands, PD-L1, has been associated with the generation of peripheral FoxP3+ regulatory T cells (pTreg), which are critical mediators of immune tolerance. Also, while PD-1 is known to be up-regulated following conventional T cell activation, we hypothesized that PD-1 might control the comparatively weak “tonic” signals T cells receive in response to self pMHC, and in the setting of LIP of newly generated T cells, this lack of restraint on normally tonic signals led to a widespread self pMHC-directed polyclonal response. We examined the role of PD-1 in the control of pTreg generation and found that deficiency of PD-1 does not preclude pTreg generation or regulatory T cell function. Instead, PD-1 is important for restraining the expansion of both conventional and regulatory cells in a lymphopenic host, suggesting that clinical approaches to tumour immunotherapy mediated by PD-1 blockade are unlikely to work by inhibiting tumour-associated pTreg generation and may actually expand them. We also found that PD-1…

Subjects/Keywords: Autoimmunity; Tolerance; Co-stimulation; Co-inhibition; Lymphopenia; T cell receptor; TCR; PD-1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ellestad, K. K. (2016). Mechanisms underlying lymphopenia-driven autoimmunity in the setting of co-inhibitory molecule deficiency. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/ckk91fk70f

Chicago Manual of Style (16^th Edition):

Ellestad, Kristofor K. “Mechanisms underlying lymphopenia-driven autoimmunity in the setting of co-inhibitory molecule deficiency.” 2016. Doctoral Dissertation, University of Alberta. Accessed March 06, 2021. https://era.library.ualberta.ca/files/ckk91fk70f.

MLA Handbook (7^th Edition):

Ellestad, Kristofor K. “Mechanisms underlying lymphopenia-driven autoimmunity in the setting of co-inhibitory molecule deficiency.” 2016. Web. 06 Mar 2021.

Vancouver:

Ellestad KK. Mechanisms underlying lymphopenia-driven autoimmunity in the setting of co-inhibitory molecule deficiency. [Internet] [Doctoral dissertation]. University of Alberta; 2016. [cited 2021 Mar 06]. Available from: https://era.library.ualberta.ca/files/ckk91fk70f.

Council of Science Editors:

Ellestad KK. Mechanisms underlying lymphopenia-driven autoimmunity in the setting of co-inhibitory molecule deficiency. [Doctoral Dissertation]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/ckk91fk70f

4. Qian, Chengrui. Etude des intéractions entre les étapes précoces des voies de signalisation dépendantes du TCR et de CD28 dans l'initiation de l'activation des lymphocytes T naïfs : Study of the interaction between the early stages of signal dependant on TCR and CD28 in the initiation activation of naive T cells.

Degree: Docteur es, Immunologie, 2013, Aix Marseille Université

URL: http://www.theses.fr/2013AIXM4058

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L'activation des lymphocytes T est initié à la fois de TCR et l'engagement du co-récepteur. CD28 est le plus important sur les cellules T naïves.… (more)

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L'activation des lymphocytes T est initié à la fois de TCR et l'engagement du co-récepteur. CD28 est le plus important sur les cellules T naïves. Cette activation doit être strictement réglementé, depuis son apparition inexacte pourrait être de conséquences néfastes. Nous avons signalé que TCR et CD28 début signalisation génèrent mécanisme de détection de coïncidence dans l'initiation de l'activation des cellules T naïves. Tout d'abord, nous avons constaté que TCR déclenchement avec ligand apparenté pMHC ou anticorps augmente considérablement la liaison 2D du CD28 à ses ligands B7 et dépend à la fois la queue cytoplasmique de CD28 et l'activité de Src kinases. En outre, on a observé une interaction TCR-pMHC pour améliorer la phosphorylation sur tyrosine de CD28 induite lors de l'engagement de B7. L'analyse du récepteur déclenché par événements de signalisation dans les cellules CD4 + naïves cellules T ont montré que seul TCR ou la stimulation de CD28 est seulement capable d'induire une Ca2 faible ou minimal + réponse en dépit de la phospholipase facilement détectée C- une phosphorylation, mais la stimulation concomitante des deux voies suscité efficacement forte et soutenue + entrée Ca2 impliquant les canaux CRAC. Ainsi, notre étude a révélé apparition de la détection de coïncidence à deux étapes importantes au cours de la TCR et CD28 déclenchée par l'activation des cellules T naïves, se liant à savoir le ligand et le déclenchement des récepteurs et la mobilisation intracellulaire, qui fournit d'importantes nouvelles connaissances sur le mécanisme de l'initiation de la réponse immunitaire primaire, ainsi que sa régulation.

T cell activation is initiated by signaling pathways triggered upon ligand engagement of the TCR and co-stimulatory receptors, respectively, with CD28 being the major one among the latter class of molecules on naïve T cells. At the same time, such activation needs to be tightly regulated, since its improper occurrence might be of detrimental consequences. We report here that interactions between TCR and CD28 early signaling pathways generate coincidence detection mechanism in the initiation of naïve T cell activation. First, we found that in naïve CD4+ T cells, TCR engagement with pMHC cognate ligand or antibody significantly increases the 2D binding of CD28 to its B7 ligands and this increase depends on both the cytoplasmic tail of CD28 and activity of src kinases. Moreover, TCR-pMHC interaction was observed to enhance the tyrosine phosphorylation of CD28 induced upon B7 engagement. Analysis of the receptor-triggered signaling events in naïve CD4+ T cells showed that alone TCR or CD28 stimulation was only capable of inducing a weak or minimal Ca2+ response in spite of the readily detected phospholipase C-1 phosphorylation, but the concurrent stimulation of both pathways efficiently elicited strong and sustained Ca2+ mobilization involving the CRAC channels. Our study has thus uncovered occurrence of the coincidence detection at two major steps during the TCR- and CD28-triggered activation…

Advisors/Committee Members: He, Hai Tao (thesis director).

Subjects/Keywords: L'activation des cellules T; Co-stimulation; Signling de calcium; Adhésion cellulaire; T cell activation; Co-stimulation; Calcium signling; Cell adhesion; 571

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qian, C. (2013). Etude des intéractions entre les étapes précoces des voies de signalisation dépendantes du TCR et de CD28 dans l'initiation de l'activation des lymphocytes T naïfs : Study of the interaction between the early stages of signal dependant on TCR and CD28 in the initiation activation of naive T cells. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2013AIXM4058

Chicago Manual of Style (16^th Edition):

Qian, Chengrui. “Etude des intéractions entre les étapes précoces des voies de signalisation dépendantes du TCR et de CD28 dans l'initiation de l'activation des lymphocytes T naïfs : Study of the interaction between the early stages of signal dependant on TCR and CD28 in the initiation activation of naive T cells.” 2013. Doctoral Dissertation, Aix Marseille Université. Accessed March 06, 2021. http://www.theses.fr/2013AIXM4058.

MLA Handbook (7^th Edition):

Qian, Chengrui. “Etude des intéractions entre les étapes précoces des voies de signalisation dépendantes du TCR et de CD28 dans l'initiation de l'activation des lymphocytes T naïfs : Study of the interaction between the early stages of signal dependant on TCR and CD28 in the initiation activation of naive T cells.” 2013. Web. 06 Mar 2021.

Vancouver:

Qian C. Etude des intéractions entre les étapes précoces des voies de signalisation dépendantes du TCR et de CD28 dans l'initiation de l'activation des lymphocytes T naïfs : Study of the interaction between the early stages of signal dependant on TCR and CD28 in the initiation activation of naive T cells. [Internet] [Doctoral dissertation]. Aix Marseille Université 2013. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2013AIXM4058.

Council of Science Editors:

Qian C. Etude des intéractions entre les étapes précoces des voies de signalisation dépendantes du TCR et de CD28 dans l'initiation de l'activation des lymphocytes T naïfs : Study of the interaction between the early stages of signal dependant on TCR and CD28 in the initiation activation of naive T cells. [Doctoral Dissertation]. Aix Marseille Université 2013. Available from: http://www.theses.fr/2013AIXM4058

University of Toronto

5. Lin, Gloria Hoi Ying. Dissecting the Role of 4-1BB and its Ligand in Enhancing CD8 Effector and Memory T Cell Responses.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/32077

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The Tumor necrosis factor receptor (TNFR) family member 4-1BB and its TNF family ligand, 4-1BBL, are important in modulating multiple stages of the CD8 T… (more)

Subjects/Keywords: 4-1BB; CD8 T cells; Influenza; Tumor immunotherapy; CD8 memory T cells; Co-stimulation; 0982

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, G. H. Y. (2011). Dissecting the Role of 4-1BB and its Ligand in Enhancing CD8 Effector and Memory T Cell Responses. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/32077

Chicago Manual of Style (16^th Edition):

Lin, Gloria Hoi Ying. “Dissecting the Role of 4-1BB and its Ligand in Enhancing CD8 Effector and Memory T Cell Responses.” 2011. Doctoral Dissertation, University of Toronto. Accessed March 06, 2021. http://hdl.handle.net/1807/32077.

MLA Handbook (7^th Edition):

Lin, Gloria Hoi Ying. “Dissecting the Role of 4-1BB and its Ligand in Enhancing CD8 Effector and Memory T Cell Responses.” 2011. Web. 06 Mar 2021.

Vancouver:

Lin GHY. Dissecting the Role of 4-1BB and its Ligand in Enhancing CD8 Effector and Memory T Cell Responses. [Internet] [Doctoral dissertation]. University of Toronto; 2011. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1807/32077.

Council of Science Editors:

Lin GHY. Dissecting the Role of 4-1BB and its Ligand in Enhancing CD8 Effector and Memory T Cell Responses. [Doctoral Dissertation]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/32077

University of Toronto

6. Clouthier, Derek Leonard. The T Cell Co-stimulatory Molecule GITR in the Control and Treatment of a Persistent Viral Infection.

Degree: PhD, 2015, University of Toronto

URL: http://hdl.handle.net/1807/70827

► During persistent viral infections such as human immunodeficiency virus (HIV) in human or lymphocytic choriomeningitis virus (LCMV) in mice, the immune response must achieve a… (more)

▼ During persistent viral infections such as human immunodeficiency virus (HIV) in human or lymphocytic choriomeningitis virus (LCMV) in mice, the immune response must achieve a balance between immune control and pathology. CD4 T cell help and co-stimulatory factors remain under-investigated in this context. This thesis explores the role of the Glucocorticoid-Induced Tumour Necrosis Factor Receptor-Related Protein (GITR) and its efficacy as a target for therapy in chronic infection. Mice that lack the T cell co-stimulatory molecule GITR have impaired LCMV-specific CD8 T cell responses and control of chronic LCMV infection. The effects of GITR were lost when mice were depleted of CD4 T cells. GITR directly supports the accumulation of IL-2+ T helper type 1 (Th1) cells, thereby indirectly facilitating early LCMV-specific CD8 T cell responses, late B cell responses, and viral control. In vivo GITR-induced signals were detected at day 3 post-infection, and defects in CD4 T cell accumulation in GITR-deficient T cells were apparent starting at day 5 post-infection. GITR-Ligand (GITRL) is maximally induced on antigen presenting cells at day 2 post-infection, but is downregulated to below baseline levels by day 8 post-infection, and remains so at the chronic stage of infection (day 21 post-infection). GITR expression was highest on regulatory T cells (Tregs) but was also detected on Th1 and LCMV-specific CD8 T cells at day 8 post-infection and was maintained at low levels at day 21 post-infection. As GITRL was limiting at this late time point, we investigated the potential of therapeutic stimulation of GITR using an anti-GITR agonist monoclonal antibody. Anti-GITR treatment at day 21 post-infection increased the frequency and number of LCMV-specific CD8 T cells and improved viral control. These effects of anti-GITR were CD8 T cell-intrinsic. Taken together, this thesis demonstrates that GITR plays an important early role on CD4 T cells to support CD8 T and B cell responses to persistent LCMV infection, but at later time points, anti-GITR therapy acts directly on the CD8 T cells to improve viral control. These studies may inform the development of novel immune therapies for human persistent viral infections as well as malignancies. Advisors/Committee Members: Watts, Tania H, Immunology.

Subjects/Keywords: B cells; Co-stimulation; GITR; LCMV; T cells; TNFR; 0982

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clouthier, D. L. (2015). The T Cell Co-stimulatory Molecule GITR in the Control and Treatment of a Persistent Viral Infection. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/70827

Chicago Manual of Style (16^th Edition):

Clouthier, Derek Leonard. “The T Cell Co-stimulatory Molecule GITR in the Control and Treatment of a Persistent Viral Infection.” 2015. Doctoral Dissertation, University of Toronto. Accessed March 06, 2021. http://hdl.handle.net/1807/70827.

MLA Handbook (7^th Edition):

Clouthier, Derek Leonard. “The T Cell Co-stimulatory Molecule GITR in the Control and Treatment of a Persistent Viral Infection.” 2015. Web. 06 Mar 2021.

Vancouver:

Clouthier DL. The T Cell Co-stimulatory Molecule GITR in the Control and Treatment of a Persistent Viral Infection. [Internet] [Doctoral dissertation]. University of Toronto; 2015. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1807/70827.

Council of Science Editors:

Clouthier DL. The T Cell Co-stimulatory Molecule GITR in the Control and Treatment of a Persistent Viral Infection. [Doctoral Dissertation]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/70827

University of Toronto

7. Ng, Dennis Yu Man. The Lymphotoxin/Type I IFN axis in CD8+ T cell responses.

Degree: PhD, 2014, University of Toronto

URL: http://hdl.handle.net/1807/72606

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This thesis examines the role of the TNF family member Lymphotoxin-α1β2 (LTαβ) and its cognate receptor Lymphotoxin-β receptor (LTβR) in modulating DC immunogenic functions that… (more)

Subjects/Keywords: co-stimulation; Dendritic cell; Interferon; Lymphotoxin; T cell activation; TNF; 0982

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ng, D. Y. M. (2014). The Lymphotoxin/Type I IFN axis in CD8+ T cell responses. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/72606

Chicago Manual of Style (16^th Edition):

Ng, Dennis Yu Man. “The Lymphotoxin/Type I IFN axis in CD8+ T cell responses.” 2014. Doctoral Dissertation, University of Toronto. Accessed March 06, 2021. http://hdl.handle.net/1807/72606.

MLA Handbook (7^th Edition):

Ng, Dennis Yu Man. “The Lymphotoxin/Type I IFN axis in CD8+ T cell responses.” 2014. Web. 06 Mar 2021.

Vancouver:

Ng DYM. The Lymphotoxin/Type I IFN axis in CD8+ T cell responses. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1807/72606.

Council of Science Editors:

Ng DYM. The Lymphotoxin/Type I IFN axis in CD8+ T cell responses. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/72606

Universidade do Rio Grande do Sul

8. Moura, Ana Maria Mielniczuk de. A interação entre artigos e patentes : um estudo cientométrico da comunicação científica e tecnológica em biotecnologia.

Degree: 2009, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/18561

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As etapas do trabalho constituíram-se de busca nas bases de dados; limpeza e organização dos nomes de autores e de instituições; análises estatísticas e de… (more)

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As etapas do trabalho constituíram-se de busca nas bases de dados; limpeza e organização dos nomes de autores e de instituições; análises estatísticas e de redes sociais; correlação entre a co-invenção e co-autoria, a partir da ocorrência de autores co-ativos – que publicaram tanto patentes como artigos - nos dois tipos de documentos; correlação entre os assuntos dos artigos e das patentes a partir do mapeamento dos assuntos proposto por Glänzel e Schubert (2003) e dos códigos da Classificação Internacional de Patentes (CIP). A Biotecnologia no Brasil caracteriza-se por apresentar uma forte interação entre Ciência e Tecnologia, o que significa que os inventores transitam entre as duas esferas e produzem tanto publicações científicas como tecnológicas, principalmente no âmbito da universidade. Os resultados mostram que no ranking da produção científica, lideram as universidades públicas (federais e estaduais) e instituições de pesquisa. Observou-se que há intensa colaboração entre estes dois tipos de instituições, com a formação de clusters com laços fortes, não acontecendo colaboração destas com empresas, de forma efetiva. No ranking da produção tecnológica, as empresas depositantes não figuram entre os primeiros lugares, ficando estes ainda ocupados pelas universidades, instituições de pesquisa e de fomento. Muitos depositantes e inventores encontram-se isolados nas análises de redes sociais, confirmando a predominância de redes diádicas ou a falta de formação de redes de co-autoria de patentes na área da Biotecnologia. Encontra-se um núcleo de preferência para a co-autoria em artigos situado entre 3 e 7 autores. Em patentes, os co-ativos demonstram a preferência pela produção individual ou em pequenas equipes. Os autores e instituições coativas que mais possuem patentes são também aqueles que mais publicam artigos, apresentando uma relação entre produção de artigos e patentes. A co-autoria encontrada nas publicações de patentes se repete nas publicações científicas, demonstrando uma interação entre C&T, que alcança um percentual de 70,7% de interação entre a produção científica e tecnológica. Esta interação também foi observada a partir da co-classificação, atingindo o percentual de 83,4%. As patentes que mais possuem convergência entre co-autoria e co-classificação são aquelas que possuem menor número de co-ativos. A hipótese H1, que afirmava que os autores co-ativos mais produtivos em C&T pertencem a redes de co-autoria interpessoais mais densas, não foi comprovado. Considera-se que as técnicas da cientometria utilizadas neste estudo possibilitaram a análise da interação entre C&T na área da Biotecnologia no Brasil.

The research work analyzes the interaction between science and technology (S&T) from a scientometric approach, using co-authorship and co-classification techniques. It aims to demonstrate the movement of Biotechnology researchers and inventors from Brazil across the scientific and technological spheres by means of correlating their articles and patents. The corpus comprises 2.584 articles and 194…

Advisors/Committee Members: Caregnato, Sonia Elisa.

Subjects/Keywords: Cientometria; Scientometrics; Comunicação científica; Coactivity; Co-authorship; Patentes; Co-invention; Artigo científico; Co-autoria científica; Co-classification; Article; Patent; Interaction S&T

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APA (6^th Edition):

Moura, A. M. M. d. (2009). A interação entre artigos e patentes : um estudo cientométrico da comunicação científica e tecnológica em biotecnologia. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/18561

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moura, Ana Maria Mielniczuk de. “A interação entre artigos e patentes : um estudo cientométrico da comunicação científica e tecnológica em biotecnologia.” 2009. Thesis, Universidade do Rio Grande do Sul. Accessed March 06, 2021. http://hdl.handle.net/10183/18561.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moura, Ana Maria Mielniczuk de. “A interação entre artigos e patentes : um estudo cientométrico da comunicação científica e tecnológica em biotecnologia.” 2009. Web. 06 Mar 2021.

Vancouver:

Moura AMMd. A interação entre artigos e patentes : um estudo cientométrico da comunicação científica e tecnológica em biotecnologia. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2009. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10183/18561.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moura AMMd. A interação entre artigos e patentes : um estudo cientométrico da comunicação científica e tecnológica em biotecnologia. [Thesis]. Universidade do Rio Grande do Sul; 2009. Available from: http://hdl.handle.net/10183/18561

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brigham Young University

9. Freitas, Claudia Mercedes. Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor.

Degree: PhD, 2019, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9257&context=etd

► According to the centers for disease control and prevention (CDC) and the world healthorganization (WHO), heart disease and immune related diseases such as diabetes and… (more)

▼ According to the centers for disease control and prevention (CDC) and the world healthorganization (WHO), heart disease and immune related diseases such as diabetes and cancer areamong the leading causes of death around the world. Thus, the regulation of the function ofimmune cell plays a key role in health and disease. Calcium (Ca2+) ions play a critical role inimmune cell activation, function and in a robust immune response. Defects in Ca2+ signalinginfluences the development of cardiac disease, Alzheimer disease, immune cell metabolism,muscle dysfunction, and cancer. Each immune cell is unique in its activation and function,making it relevant to understand how activation of each type of immune cell is regulated. Herewe describe the role of the nBMP2 protein in macrophage activation and function and the role ofthe CD5 co-receptor in helper T cell activation and function.The nuclear bone morphogenetic protein 2 (nBMP2) is the nuclear variant of the bonemorphogenetic protein 2 (BMP2), a growth factor important in heart development, neurogenesis,bone, cartilage and muscle development. To better understand the function of nBMP2, transgenicnBMP2 mutant mice were generated. These mice have a slow muscle relaxation and cognitivedeficit caused in part by abnormal Ca2+ mobilization. Mutant nBMP2 mice also have an impairedsecondary immune response to systemic bacterial challenge. Here we have further characterizedmacrophage activation and function from mutant nBMP2 mice before and after bacterialinfection. We describe how nBMP2 influences the Ca2+ mobilization response and phagocytosisin macrophages, revealing a novel role of the nBMP2 protein in immune cell regulation.CD5 is a surface marker on T cells, thymocytes, and the B1 subset of B cells. CD5 isknown to play an important role during thymic development of T cells. CD5 functions as anegative regulator of T cell receptor (TCR) signaling and fine tunes the TCR signaling response.Here we describe our characterization of CD5 regulation of Ca2+ signaling in naïve helper Tcells. We also outline our findings examining how CD5-induced changes in helper T cellactivation influence other biological processes such as immune cell metabolism, the diversity ofthe gut microbiome, and cognitive function and behavior. Thus, this work elucidates theinfluence of the CD5 co-receptor on the functional outcomes in multiple systems when CD5 isaltered.

Subjects/Keywords: nBMP2; bone morphogenetic protein; CD5; co-receptor; calcium (Ca2+); metabolism; behavior; TCR; T cell receptor; microbiome; macrophage; T helper cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Freitas, C. M. (2019). Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor. (Doctoral Dissertation). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9257&context=etd

Chicago Manual of Style (16^th Edition):

Freitas, Claudia Mercedes. “Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor.” 2019. Doctoral Dissertation, Brigham Young University. Accessed March 06, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9257&context=etd.

MLA Handbook (7^th Edition):

Freitas, Claudia Mercedes. “Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor.” 2019. Web. 06 Mar 2021.

Vancouver:

Freitas CM. Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor. [Internet] [Doctoral dissertation]. Brigham Young University; 2019. [cited 2021 Mar 06]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9257&context=etd.

Council of Science Editors:

Freitas CM. Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor. [Doctoral Dissertation]. Brigham Young University; 2019. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=9257&context=etd

Georgia Tech

10. Hong, Jin Sung. Study of 2D kinetics and force regulation in T cell recognition.

Degree: PhD, Mechanical Engineering, 2014, Georgia Tech

URL: http://hdl.handle.net/1853/53430

► T cell activation and thymic selection are thought to be determined by the binding propensity (avidity or affinity) of the T cell receptor (TCR) to… (more)

Subjects/Keywords: T cell recognition; T cell activation; Thymic selection; TCR-pMHC interaction; Co-receptor cooperativity; 2D kinetics; Catch bond

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APA (6^th Edition):

Hong, J. S. (2014). Study of 2D kinetics and force regulation in T cell recognition. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/53430

Chicago Manual of Style (16^th Edition):

Hong, Jin Sung. “Study of 2D kinetics and force regulation in T cell recognition.” 2014. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021. http://hdl.handle.net/1853/53430.

MLA Handbook (7^th Edition):

Hong, Jin Sung. “Study of 2D kinetics and force regulation in T cell recognition.” 2014. Web. 06 Mar 2021.

Vancouver:

Hong JS. Study of 2D kinetics and force regulation in T cell recognition. [Internet] [Doctoral dissertation]. Georgia Tech; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1853/53430.

Council of Science Editors:

Hong JS. Study of 2D kinetics and force regulation in T cell recognition. [Doctoral Dissertation]. Georgia Tech; 2014. Available from: http://hdl.handle.net/1853/53430

11. Katiusca Wessler. Sistemas de P(3HB) e P(3HB-co-3HV) com policaprolactona-triol: comportamento de fase, reologia, propriedades mecânicas e processabilidade.

Degree: 2007, Universidade do Estado de Santa Catarina

URL: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=894 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=895 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=896

► Nas últimas duas décadas, os polihidroxialcanoatos (PHAs) foram o foco de pesquisas extensivas considerando suas potenciais aplicações como termoplásticos biocompatíveis e biodegradáveis, devido a sua… (more)

Subjects/Keywords: P(3HB); P(3HB-co-3HV); PCL-T; Comportamento de fase, Reologia e processamento; POLIMEROS; P(3HB); P(3HB-co-3HV); PCL-T, Phase behavior; Reology, melt process

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APA (6^th Edition):

Wessler, K. (2007). Sistemas de P(3HB) e P(3HB-co-3HV) com policaprolactona-triol: comportamento de fase, reologia, propriedades mecânicas e processabilidade. (Thesis). Universidade do Estado de Santa Catarina. Retrieved from http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=894 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=895 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=896

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wessler, Katiusca. “Sistemas de P(3HB) e P(3HB-co-3HV) com policaprolactona-triol: comportamento de fase, reologia, propriedades mecânicas e processabilidade.” 2007. Thesis, Universidade do Estado de Santa Catarina. Accessed March 06, 2021. http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=894 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=895 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=896.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wessler, Katiusca. “Sistemas de P(3HB) e P(3HB-co-3HV) com policaprolactona-triol: comportamento de fase, reologia, propriedades mecânicas e processabilidade.” 2007. Web. 06 Mar 2021.

Vancouver:

Wessler K. Sistemas de P(3HB) e P(3HB-co-3HV) com policaprolactona-triol: comportamento de fase, reologia, propriedades mecânicas e processabilidade. [Internet] [Thesis]. Universidade do Estado de Santa Catarina; 2007. [cited 2021 Mar 06]. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=894 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=895 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=896.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wessler K. Sistemas de P(3HB) e P(3HB-co-3HV) com policaprolactona-triol: comportamento de fase, reologia, propriedades mecânicas e processabilidade. [Thesis]. Universidade do Estado de Santa Catarina; 2007. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=894 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=895 ; http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=896

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

12. Adaila, Kawther. Synthesis and characterization of Pd(II), Co(III) and Ni(II) complexes with the condenzation derivative of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent.

Degree: Hemijski fakultet, 2018, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:15496/bdef:Content/get

►

Chemistry - Inorganic chemistry / Hemija - Neorganska hemija

Complexes of Pd(II), Co(III) and Ni(II) with the condensation derivative of 2-(diphenylphosphino)benzaldehyde and Girard’s T reagent… (more)

Subjects/Keywords: Pd(II); Co(III) i Ni(II) kompleksi; 2-(difenilfosfino)benzaldehid; Žirar T reagens

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APA (6^th Edition):

Adaila, K. (2018). Synthesis and characterization of Pd(II), Co(III) and Ni(II) complexes with the condenzation derivative of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:15496/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Adaila, Kawther. “Synthesis and characterization of Pd(II), Co(III) and Ni(II) complexes with the condenzation derivative of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent.” 2018. Thesis, Univerzitet u Beogradu. Accessed March 06, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:15496/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Adaila, Kawther. “Synthesis and characterization of Pd(II), Co(III) and Ni(II) complexes with the condenzation derivative of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent.” 2018. Web. 06 Mar 2021.

Vancouver:

Adaila K. Synthesis and characterization of Pd(II), Co(III) and Ni(II) complexes with the condenzation derivative of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Mar 06]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:15496/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adaila K. Synthesis and characterization of Pd(II), Co(III) and Ni(II) complexes with the condenzation derivative of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:15496/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

13. Meilleur, Courtney. Effect of Bacterial Superantigens on the Magnitude and Breadth of Influenza-Specific CD8+ T Cell Responses.

Degree: 2018, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/5874

► Severe cases of influenza A virus (IAV) infection are often complicated by concomitant bacterial pneumonia or sepsis. Many bacterial species are capable of producing potent… (more)

Subjects/Keywords: T cell; superantigen; influenza; co-infection; vaccination; memory; Staphylococcus aureus; enterotoxin; immunity; immunodominance; Virus Diseases

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APA (6^th Edition):

Meilleur, C. (2018). Effect of Bacterial Superantigens on the Magnitude and Breadth of Influenza-Specific CD8+ T Cell Responses. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/5874

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Meilleur, Courtney. “Effect of Bacterial Superantigens on the Magnitude and Breadth of Influenza-Specific CD8+ T Cell Responses.” 2018. Thesis, University of Western Ontario. Accessed March 06, 2021. https://ir.lib.uwo.ca/etd/5874.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Meilleur, Courtney. “Effect of Bacterial Superantigens on the Magnitude and Breadth of Influenza-Specific CD8+ T Cell Responses.” 2018. Web. 06 Mar 2021.

Vancouver:

Meilleur C. Effect of Bacterial Superantigens on the Magnitude and Breadth of Influenza-Specific CD8+ T Cell Responses. [Internet] [Thesis]. University of Western Ontario; 2018. [cited 2021 Mar 06]. Available from: https://ir.lib.uwo.ca/etd/5874.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meilleur C. Effect of Bacterial Superantigens on the Magnitude and Breadth of Influenza-Specific CD8+ T Cell Responses. [Thesis]. University of Western Ontario; 2018. Available from: https://ir.lib.uwo.ca/etd/5874

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

14. Reid, Michael John Charles. Infectious Agent Evolution in Bornean Orangutans.

Degree: PhD, 2016, University of Toronto

URL: http://hdl.handle.net/1807/76801

► In this thesis I provide among the first detailed studies of orangutan host/parasite co-evolution by bridging the disciplines of molecular virology/parasitology and biological anthropology. I… (more)

Subjects/Keywords: Co-Evolution; Evolution; Orangutans; Plasmodium; Simian Foamy Virus; Simian T-Cell Lymphotropic Virus 1; 0327

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APA (6^th Edition):

Reid, M. J. C. (2016). Infectious Agent Evolution in Bornean Orangutans. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/76801

Chicago Manual of Style (16^th Edition):

Reid, Michael John Charles. “Infectious Agent Evolution in Bornean Orangutans.” 2016. Doctoral Dissertation, University of Toronto. Accessed March 06, 2021. http://hdl.handle.net/1807/76801.

MLA Handbook (7^th Edition):

Reid, Michael John Charles. “Infectious Agent Evolution in Bornean Orangutans.” 2016. Web. 06 Mar 2021.

Vancouver:

Reid MJC. Infectious Agent Evolution in Bornean Orangutans. [Internet] [Doctoral dissertation]. University of Toronto; 2016. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1807/76801.

Council of Science Editors:

Reid MJC. Infectious Agent Evolution in Bornean Orangutans. [Doctoral Dissertation]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/76801

University of Cambridge

15. Smid, Andrei Iosif. Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment.

Degree: PhD, 2019, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/297674

► T-cell development is a complex multi-step process, driving the maturation of hematopoietic stem cells into fully functional mature T-cells that play a major role in… (more)

▼ T-cell development is a complex multi-step process, driving the maturation of hematopoietic stem cells into fully functional mature T-cells that play a major role in the adaptive immune response against pathogens. This developmental process is regulated by a series of checkpoints in which the signalling capacity of the T-cell antigen receptor (TCR) is tested. One such checkpoint is the generation of the clonotypic chains of the two TCR variants (αβ-TCR or γδ-TCR) by V(D)J recombination. Importantly, the TCRα chain is rearranged only after correct TCRβ expression, which requires the latter chain to pair with a surrogate invariant pre-Tα chain to form the pre-TCR complex. While both TCR variants activate the same signalling pathway, they commit the T cells to distinct functional lineages. Insight into how thymocytes are nonetheless able to distinguish between these two signals is crucial to our fundamental understanding of T-cell development and how it might be manipulated therapeutically. Pertinently, the pre-TCR is undetectable at the cell surface, unlike the γδ TCR which is primarily located at the plasma membrane. In this thesis, I aim to investigate whether the distinct intracellular localisation of the pre-TCR provides a mean for this signal to be distinguished from that of the γδ-TCR. Through the reconstitution of pre-TCR expression in a non-immune cell, I show that although the pre-TCR can in fact translocate to the cell surface, it is rapidly removed via clathrin-mediated endocytosis and transported to lysosomes. The use of a non-immune cell permits the study of pre-TCR trafficking in the absence of other signalling components and demonstrates that this intracellular localisation of the pre-TCR is intrinsic to the receptor. Using a panel of truncated and engineered pre-TCR, I demonstrate that the absence of a second Ig domain in the extracellular domain of the pre-Tα chain promotes the rapid endocytosis of the receptor. In a thymocyte-OP9-DL1 co-culture system, I show that recombination-deficient thymocytes expressing a pre-TCR variant lacking the TCRβ variable domain is sufficient to drive cells through the TCRβ-selection checkpoint and give rise to double positive cells. Likewise, wild type thymocytes over-expressing the pre-TCR or a receptor with a fused TCRα variable domain may push thymocytes down the γδ-lineage. Finally, I employ a biotin proximity-labelling assay to screen for binding partners of the pre-TCR that might mediate its internalisation. Using this method, I identify TMEM131, a novel protein with an unknown function and characterise some of its biochemical and cell biological properties.

Subjects/Keywords: T-cell; T-cell development; Pre-T-cell receptor; Trafficking; TCR signalling; γδ-TCR; OP9-DL1 co-culture; hematopoietic stem cells; BioID2

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APA (6^th Edition):

Smid, A. I. (2019). Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/297674

Chicago Manual of Style (16^th Edition):

Smid, Andrei Iosif. “Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment.” 2019. Doctoral Dissertation, University of Cambridge. Accessed March 06, 2021. https://www.repository.cam.ac.uk/handle/1810/297674.

MLA Handbook (7^th Edition):

Smid, Andrei Iosif. “Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment.” 2019. Web. 06 Mar 2021.

Vancouver:

Smid AI. Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Mar 06]. Available from: https://www.repository.cam.ac.uk/handle/1810/297674.

Council of Science Editors:

Smid AI. Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/297674

Georgia State University

16. Kumari, Anita. Radiation epigenetically modulates tumor cells and alters activation and function of effector T cells.

Degree: PhD, Biology, 2015, Georgia State University

URL: https://scholarworks.gsu.edu/biology_diss/161

► Radiation is a common therapeutic modality for cancer however it fails to control advanced and malignant disease. As a result, novel approaches that aim… (more)

▼ Radiation is a common therapeutic modality for cancer however it fails to control advanced and malignant disease. As a result, novel approaches that aim to stimulate immune attack of tumors are currently being investigated and approved by the US FDA. It is clear, however, that no single agent will be responsible for achieving long-term control and treatment of cancer and that combination therapies will be required. Previous studies indicate that colorectal cancer cells that survive radiation up-regulate surface expression of cytotoxic T lymphocyte (CTL) relevant proteins including death receptors, cell adhesion molecules and tumor-associated antigens (TAA). The aim of this dissertation was to investigate the effect of non-cytolytic doses of ionizing radiation (IR) on co-stimulatory molecule expression on tumor cells and evaluate the impact of their modulation on effector T-cell biology and tumoricidal activity. Here, several human tumor cell lines were exposed to various doses of radiation (0-10Gy) and TAA-specific T-cell tumoricidal activity and expression of effector CTL co-stimulatory molecules were evaluated. I found OX40L and 41BBL to be the most consistently upregulated proteins post-IR by flow cytometry. Furthermore, I saw enhanced survival and activation of human CD8+ T-cells exposed to irradiated tumor cells. Importantly, enhanced killing of irradiated tumor cells by TAA-specific CTLs in cytotoxicity assays was reported. Blocking OX40L and 41BBL reversed radiation-enhanced T-cell killing. My data also indicate that expression of 41BBL and OX40L can be epigenetically regulated, as inhibition of histone deacetylases (HDAC) and of DNA methyltransferases (DNMT) resulted in increased OX40L and 41BBL mRNA and protein expression. Furthermore, chromatin immunoprecipitation experiments revealed increased histone H3 acetylation specifically at the 41BBL promoter following irradiation. Last, I began exploring the ability of IR to reverse immune suppression by evaluating the impact of radiation on regulatory T cells (T_REGS)that can suppress the function of effector CTLs. I found that RT could reduce T_REG numbers likely by altering their phenotype. Overall, this dissertation demonstrates that radiation can be used to make human tumors more immunogenic through epigenetic modulation of genes stimulatory to effector T-cells and that it may also reverse immune suppression by phenotypically altering T_REGcells. Advisors/Committee Members: Charlie Benson.

Subjects/Keywords: radiation therapy; cancer immunotherapy; ionizing irradiation; immunogenic modulation; CTLs; epigenetic; effector co- stimulation; and regulatory T cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kumari, A. (2015). Radiation epigenetically modulates tumor cells and alters activation and function of effector T cells. (Doctoral Dissertation). Georgia State University. Retrieved from https://scholarworks.gsu.edu/biology_diss/161

Chicago Manual of Style (16^th Edition):

Kumari, Anita. “Radiation epigenetically modulates tumor cells and alters activation and function of effector T cells.” 2015. Doctoral Dissertation, Georgia State University. Accessed March 06, 2021. https://scholarworks.gsu.edu/biology_diss/161.

MLA Handbook (7^th Edition):

Kumari, Anita. “Radiation epigenetically modulates tumor cells and alters activation and function of effector T cells.” 2015. Web. 06 Mar 2021.

Vancouver:

Kumari A. Radiation epigenetically modulates tumor cells and alters activation and function of effector T cells. [Internet] [Doctoral dissertation]. Georgia State University; 2015. [cited 2021 Mar 06]. Available from: https://scholarworks.gsu.edu/biology_diss/161.

Council of Science Editors:

Kumari A. Radiation epigenetically modulates tumor cells and alters activation and function of effector T cells. [Doctoral Dissertation]. Georgia State University; 2015. Available from: https://scholarworks.gsu.edu/biology_diss/161

University of Vienna

17. Nussbaum, Nicole. In vitro development of tissue resident memory T cells from circulating precursor lymphocytes.

Degree: 2020, University of Vienna

URL: http://othes.univie.ac.at/60657/

►

Tissue resident memory T cells, zu Deutsch Gewebsresidente Gedächtnis-T-Zellen (Trm), sind eine erst kürzlich identifizierte, funktionell unterschiedliche Untergruppe von Gedächtnis-T-Lymphozyten, die in vielen Geweben und… (more)

▼

Tissue resident memory T cells, zu Deutsch Gewebsresidente Gedächtnis-T-Zellen (Trm), sind eine erst kürzlich identifizierte, funktionell unterschiedliche Untergruppe von Gedächtnis-T-Lymphozyten, die in vielen Geweben und Organen unseres Körpers zu finden ist. In den meisten Geweben lassen sich Trm-Zellen durch die Co-Expression der Zelloberflächenmarker CD69 und CD103 identifizieren. Beide Oberflächenproteine sind für die Verankerung im Gewebe zuständig. Im Gegensatz zu anderen Gedächtnis-T-Zellen, zirkulieren Trm-Zellen nämlich nicht und verbleiben jahrelang in ihren jeweiligen Nischen. Dort tragen sie eine wichtige Funktion zur Immunüberwachung und -abwehr bei. Nachdem sie bereits ein bestimmtes Antigen erkannt haben, zeigen sie eine spezifische Immunantwort und reagieren daher schnell auf virale und bakterielle Neuinfektionen. Darüber hinaus rekrutieren Trm-Zellen andere Immunzellen zur Infektionsstelle und zeigen ein anti-tumorales Verhalten bei manchen Krebsarten. All das macht Trm- Zellen interessant für neuartige Immuntherapie- und Impfansätze. Jedoch sind viele Mechanismen und molekulare Regulationen bezüglicher ihrer Entstehung und Aufrechterhaltung, nicht vollständig verstanden. In den folgenden Studien analysierten wir die Expression von CD69 und CD103 während CD8+ T-Zell-Aktivierung in dem Modellorganismus Maus. Wir konnten zeigen, dass CD103 unter den verschiedenen T-Zell Subtypen unterschiedlich stark exprimiert wird. Darüber hinaus haben wir in verschiedenen in vitro Ansätzen den Einfluss von extrinsischen Faktoren getestet, die die Zelldifferenzierung von Trm- Zellen beeinflussen. Unterschiedliche Bedingungen und Wechselwirkungen zwischen Zelltypen wurden getestet und die Expression der Trm-Zelloberflächenmarker CD103 und CD69 mittels Mehrfarben-Durchflusszytometrie analysiert. Auf diese Weise wollten wir komplexe zelluläre Wechselwirkungen auf einfachere Weise untersuchen. Wir konnten zeigen, dass die Expression von CD69 stark von T-Zell-Rezeptor-vermittelten Signalen abhängt. Darüber hinaus kann TGF-β naive CD8+ Vorläufer-T-Zellen durch Hochregulieren von CD103 dazu bringen, einen Trm-ähnlichen Phänotypen anzunehmen. Als CD8+ T-Zell-Wechselwirkungen in Co-Kultivierungsstudien mit dendritischen Zellen und regulatorischen T-Zellen (Tregs) getestet wurden, konnte ein potentieller externer Einfluss gezeigt werden.

Tissue resident memory T cells (Trm) are a recently identified, functionally distinct subset of memory T lymphocytes that reside in various tissues of the body. Unlike other memory T cell populations, Trm cells are non-circulating and persist in their respective niches for years. While heterogeneity is reported in different niches, the co-expression of the cell surface markers CD69 and CD103, which are associated with tissue-retention, are reliable markers for CD8+ Trm cells at epithelial sites. Over the last years, their key role in immune surveillance and defence became widely acknowledged. Being antigen-specific, they show specific immune-responses and act quick upon viral and…

Subjects/Keywords: 42.13 Molekularbiologie; T-Zelle / Gedächtniszellen / gewebsresident / CD8+ / Tregs / Dendritische Zelle / Immunologie / Zellkultur / in vitro; CD8+ T cells / Trm / Tregs / dendritic cells / co-culture / cell culture / in vitro / Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nussbaum, N. (2020). In vitro development of tissue resident memory T cells from circulating precursor lymphocytes. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/60657/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nussbaum, Nicole. “In vitro development of tissue resident memory T cells from circulating precursor lymphocytes.” 2020. Thesis, University of Vienna. Accessed March 06, 2021. http://othes.univie.ac.at/60657/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nussbaum, Nicole. “In vitro development of tissue resident memory T cells from circulating precursor lymphocytes.” 2020. Web. 06 Mar 2021.

Vancouver:

Nussbaum N. In vitro development of tissue resident memory T cells from circulating precursor lymphocytes. [Internet] [Thesis]. University of Vienna; 2020. [cited 2021 Mar 06]. Available from: http://othes.univie.ac.at/60657/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nussbaum N. In vitro development of tissue resident memory T cells from circulating precursor lymphocytes. [Thesis]. University of Vienna; 2020. Available from: http://othes.univie.ac.at/60657/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Zhai, Yunhao. Quantitative interactomics of PD-1 and BTLA in primary T cells provides a rationale for concomitant coinhibitor blockade in cancer immunotherapy : L'interactomique quantitative des molécules co-inhibitrices PD-1 et de BTLA dans les cellules T primaires justifie leur blocage concomitant dans l'immunothérapie du cancer.

Degree: Docteur es, Immunologie, 2019, Aix Marseille Université

URL: http://www.theses.fr/2019AIXM0250

►

Parmi les récepteurs co-inhibiteurs des cellules T, PD-1 et BTLA sont liés de manière évolutive. Afin de déterminer si PD-1 et BTLA exerçent des fonctions… (more)

▼

Parmi les récepteurs co-inhibiteurs des cellules T, PD-1 et BTLA sont liés de manière évolutive. Afin de déterminer si PD-1 et BTLA exerçent des fonctions redondantes, nous avons développé et validé deux modèles de souris génétiquement modifiées dans lesquelles les molécules PD-1 et BTLA expriment une étiquette génétique à leur extrémité carboxy-terminale. Ceci permet de pouvoir étudier par AP-MS les signalosomes s’assemblant autour de PD-1 et BTLA dans des cellules T primaires. Nous avons en parallèle établi un modèle ‘in vitro’ basé sur des cellules présentatrices d'antigènes et des cellules T afin d’étudier le signalosome de PD-1 dans des conditions de stimulation plus physiologiques.Sur la base de ces deux approches complémentaires, nous avons résolu la controverse existante concernant le rôle des protéines tyrosine phosphatases SHP-1 et SHP-2 dans la médiation de la co-inhibition PD-1. PD-1 recrute principalement SHP-2, mais lorsqu'il est absent, il recrute SHP-1 et reste fonctionnel. En revanche, BTLA recrute principalement SHP-1 et, dans une moindre mesure, SHP-2. En analysant séparément les complexes PD-1-SHP-1 et PD-1-SHP-2, nous avons montré que chacun d’eux est capable d’atténuer la fonction des voies de signalisation du TCR et du CD28. En conclusion, notre travail de thèse montre comment la comparaison des mécanismes de signalisation des récepteurs co-inhibiteurs via une approche d’interactomique quantitative dans des cellules T primaires révèle l'ampleur de leur redondance et fournit une justification pour la conception de combinaisons d'anticorps bloquants anti-PD-1 et anti-BTLA dans l'immunothérapie des cancers.

Among T cell coinhibitory receptors, PD-1 and BTLA are evolutionary related and coexpressed on tumor-antigen specific CD8+ T cells. Accordingly, BTLA can likely substitute for PD-1 in conditions where immune-checkpoint inhibitors target PD-1. To investigate whether PD-1 and BTLA exert redundant functions during T cell responses, we developed and validated two gene targeted mouse models in which PD-1 and BTLA molecules expressed a genetic tag at their carboxyl terminus. These mice permitted us to perform AP-MS analysis of PD-1 and BTLA molecules and defined at an unprecedented level of resolution the composition and dynamics of the PD-1 and BTLA coinhibitory signalosomes in primary effector T cells. We also established a T cell- antigen presenting cell system to study the PD-1 signalosome in more physiological stimulation condition.Based on these two complementary approaches, we solved the existing controversy regarding the role of the SHP-1 and SHP-2 protein-tyrosine phosphatases in mediating PD-1 coinhibition. PD-1 predominantly recruits SHP-2, but when absent, it recruits SHP-1 and remains functional. In contrast, BTLA predominantly recruits SHP-1 and to a lower extent SHP-2. By separately analysing the PD-1-SHP-1 and PD-1-SHP-2 complexes, we show that both dampen the TCR and CD28 signalling pathways equally. Therefore, our study illustrates how comparison of coinhibitory receptor…

Advisors/Committee Members: Malissen, Bernard (thesis director).

Subjects/Keywords: Co-Inhibiteur des cellules T; Interactomique quantitative; Immunothérapie du cancer; Pd-1; Btla; Tcr; Cd28; T cell coinhibitor; Quantitative interactomics; Cancer immunotherapy; Pd-1; Btla; Tcr; Cd28; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhai, Y. (2019). Quantitative interactomics of PD-1 and BTLA in primary T cells provides a rationale for concomitant coinhibitor blockade in cancer immunotherapy : L'interactomique quantitative des molécules co-inhibitrices PD-1 et de BTLA dans les cellules T primaires justifie leur blocage concomitant dans l'immunothérapie du cancer. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2019AIXM0250

Chicago Manual of Style (16^th Edition):

Zhai, Yunhao. “Quantitative interactomics of PD-1 and BTLA in primary T cells provides a rationale for concomitant coinhibitor blockade in cancer immunotherapy : L'interactomique quantitative des molécules co-inhibitrices PD-1 et de BTLA dans les cellules T primaires justifie leur blocage concomitant dans l'immunothérapie du cancer.” 2019. Doctoral Dissertation, Aix Marseille Université. Accessed March 06, 2021. http://www.theses.fr/2019AIXM0250.

MLA Handbook (7^th Edition):

Zhai, Yunhao. “Quantitative interactomics of PD-1 and BTLA in primary T cells provides a rationale for concomitant coinhibitor blockade in cancer immunotherapy : L'interactomique quantitative des molécules co-inhibitrices PD-1 et de BTLA dans les cellules T primaires justifie leur blocage concomitant dans l'immunothérapie du cancer.” 2019. Web. 06 Mar 2021.

Vancouver:

Zhai Y. Quantitative interactomics of PD-1 and BTLA in primary T cells provides a rationale for concomitant coinhibitor blockade in cancer immunotherapy : L'interactomique quantitative des molécules co-inhibitrices PD-1 et de BTLA dans les cellules T primaires justifie leur blocage concomitant dans l'immunothérapie du cancer. [Internet] [Doctoral dissertation]. Aix Marseille Université 2019. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2019AIXM0250.

Council of Science Editors:

Zhai Y. Quantitative interactomics of PD-1 and BTLA in primary T cells provides a rationale for concomitant coinhibitor blockade in cancer immunotherapy : L'interactomique quantitative des molécules co-inhibitrices PD-1 et de BTLA dans les cellules T primaires justifie leur blocage concomitant dans l'immunothérapie du cancer. [Doctoral Dissertation]. Aix Marseille Université 2019. Available from: http://www.theses.fr/2019AIXM0250

19. Δούμπα, Πολυξένη. Αλληλεπίδραση της συγκαλλιέργειας καρκινικών ηπατοκυττάρων με ομόλογα περιφερικά λεμφοκύτταρα in vitro.

Degree: 2013, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/40335

►

Background: Many studies have suggested that the immune response may play a crucial role in the progression of hepatocellular carcinoma (HCC). Therefore, our aim was… (more)

▼

Background: Many studies have suggested that the immune response may play a crucial role in the progression of hepatocellular carcinoma (HCC). Therefore, our aim was to establish a (i) functional culture of primary human tumor hepatocytes and non-tumor from patients with hepatocellular carcinoma (HCC) and (ii) a co-culture system of HCC and non-HCC hepatocytes with autologous peripheral blood mononuclear cells (PBMCs) in order to study in vitro cell-to-cell interactions.Methods: Tumor (HCC) and non-tumor (non-HCC) hepatocytes were isolated from the liver resection specimens of 11 patients operated for HCC, while PBMCs were retrieved immediately prior to surgery. Four biopsies were obtained from patients with no liver disease who had surgery for non malignant tumor (normal hepatocytes). Hepatocytes were either cultured alone (monoculture) or co-cultured with PBMCs. Flow cytometry measurements for MHC class II expression, apoptosis, necrosis and viability (7AAD) were performed 24 h, 48 h and 72 h in co-culture and monocultures.Results: HCC and non-HCC hepatocytes exhibited increased MHC-II expression at 48h and 72h in co-culture with PBMCs as compared to monoculture, with MHC II-expressing HCC hepatocytes showing increased viability at 72 h. PBMCs showed increased MHC-II expression (activation) in co-culture with HCC as compared to non-HCC hepatocytes at all time points. Moreover, CD8+ T cells had significantly increased apoptosis and necrosis at 48h in co-culture with HCC hepatocytes as compared to monocultures. Interestingly, MHC-II expression on both HCC and non-HCC hepatocytes in co-culture was positively correlated with the respective activated CD8+ T cells.Conclusions: We have established an in vitro co-culture model to study interactions between autologous PBMCs and primary HCC and non-HCC hepatocytes. This direct interaction leads to increased antigen presenting ability of HCC hepatocytes, activation of PBMCs with a concomitant apoptosis of activated CD8+ T cells. Although, a partially effective immune response against HCC exists, still tumor hepatocytes manage to escape.

Σκοπός/Εισαγωγή.Πολλές μελέτες έχουν προτείνει ότι η ανοσιακή απόκριση παίζει σημαντικό και καθοριστικό ρόλο στην δημιουργία καθώς και στην εξέλιξη του ηπατοκυτταρικού καρκίνου (ΗΚΚ), αλλά υπάρχει έλλειψη κατάλληλων πειραματικών μοντέλων βασισμένων στα ανθρώπινα ηπατοκύτταρα έτσι ώστε να μελετηθούν με επιτυχία οι υποκείμενοι μηχανισμοί. Επομένως, ο στόχος μας ήταν να δημιουργήσουμε (α) ένα λειτουργικό σύστημα καλλιέργειας πρωτοταγών ανθρώπινων ηπατοκυττάρων από ασθενείς με ΗΚΚ και (β) ένα σύστημα συγκαλλιέργειας ανθρώπινων καρκινικών και μη-καρκινικών ηπατοκυττάρων με αυτόλογα μονοπύρηνα κύτταρα του περιφερικού αίματος με σκοπό να μελετήσουμε τις in vitro κυτταρικές αλληλεπιδράσεις τους.Μεθοδολογία.Καρκινικά (όγκος) και μη-καρκινικά (πέριξ του όγκου) ηπατοκύτταρα απομονώθηκαν από ιστοτεμάχια ήπατος τα οποία προέρχονταν από 11 ασθενείς με ΗΚΚ που υποβλήθηκαν σε μερική ηπατεκτομή, με υποκείμενη νόσο, ένω η λήψη του αυτόλογου περιφερικού…

Subjects/Keywords: Συγκαλλιέργεια καρκινικά μονοκύτταρα - περιφερικά μονοπύρανα κύτταρα; Συγκαλλιέργεια μη-καρκινικά μονοκύτταρα - περιφερικά μονοπύρανα κύταρα; Έκφραση COB+ T λεμφοκυττάρων; Απόπτωση; Έκφραση των MHC τάξης II μορίων; HCC hepatocute - PBMCS co-cultures; Non-HCC hepatocute - PBMCS co-cultures; COB+ T lymphocyte; Apoptosis; MHC-ΙΙ exression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Δούμπα, . �. (2013). Αλληλεπίδραση της συγκαλλιέργειας καρκινικών ηπατοκυττάρων με ομόλογα περιφερικά λεμφοκύτταρα in vitro. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/40335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Δούμπα, Πολυξένη. “Αλληλεπίδραση της συγκαλλιέργειας καρκινικών ηπατοκυττάρων με ομόλογα περιφερικά λεμφοκύτταρα in vitro.” 2013. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 06, 2021. http://hdl.handle.net/10442/hedi/40335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Δούμπα, Πολυξένη. “Αλληλεπίδραση της συγκαλλιέργειας καρκινικών ηπατοκυττάρων με ομόλογα περιφερικά λεμφοκύτταρα in vitro.” 2013. Web. 06 Mar 2021.

Vancouver:

Δούμπα �. Αλληλεπίδραση της συγκαλλιέργειας καρκινικών ηπατοκυττάρων με ομόλογα περιφερικά λεμφοκύτταρα in vitro. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10442/hedi/40335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Δούμπα �. Αλληλεπίδραση της συγκαλλιέργειας καρκινικών ηπατοκυττάρων με ομόλογα περιφερικά λεμφοκύτταρα in vitro. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. Available from: http://hdl.handle.net/10442/hedi/40335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. DU SHOUHUI. CO-EXPANSION OF GAMMA DELTA T CELLS AND CYTOKINE INDUCED KILLER CELLS FOR ADOPTIVE IMMUNE CELL THERAPY.

Degree: 2015, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/122772

Subjects/Keywords: gamma delta T; CIK; CAR-T; adoptive T cell therapy; co-expansion; feeder layer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SHOUHUI, D. (2015). CO-EXPANSION OF GAMMA DELTA T CELLS AND CYTOKINE INDUCED KILLER CELLS FOR ADOPTIVE IMMUNE CELL THERAPY. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/122772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SHOUHUI, DU. “CO-EXPANSION OF GAMMA DELTA T CELLS AND CYTOKINE INDUCED KILLER CELLS FOR ADOPTIVE IMMUNE CELL THERAPY.” 2015. Thesis, National University of Singapore. Accessed March 06, 2021. http://scholarbank.nus.edu.sg/handle/10635/122772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SHOUHUI, DU. “CO-EXPANSION OF GAMMA DELTA T CELLS AND CYTOKINE INDUCED KILLER CELLS FOR ADOPTIVE IMMUNE CELL THERAPY.” 2015. Web. 06 Mar 2021.

Vancouver:

SHOUHUI D. CO-EXPANSION OF GAMMA DELTA T CELLS AND CYTOKINE INDUCED KILLER CELLS FOR ADOPTIVE IMMUNE CELL THERAPY. [Internet] [Thesis]. National University of Singapore; 2015. [cited 2021 Mar 06]. Available from: http://scholarbank.nus.edu.sg/handle/10635/122772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SHOUHUI D. CO-EXPANSION OF GAMMA DELTA T CELLS AND CYTOKINE INDUCED KILLER CELLS FOR ADOPTIVE IMMUNE CELL THERAPY. [Thesis]. National University of Singapore; 2015. Available from: http://scholarbank.nus.edu.sg/handle/10635/122772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

21. Lin, Kung-hsuan. Performance Analysis of Dual-hop Selective Relaying Scheme with Co-Channel Interferences in Asymmetric Channels.

Degree: Master, Communications Engineering, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0810114-134143

► In this paper, we consider a two-hop multiple variable AF relaying scheme. The source node is equipped with multiple antennas and precoded by orthogonal space-time… (more)

Subjects/Keywords: relay selection; transmit beamforming(T-BF); orthogonal space-time block code(OSTBC); outage probability; amplify-and-forward(AF); cooperative communication network; co-channel interference(CCI)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, K. (2014). Performance Analysis of Dual-hop Selective Relaying Scheme with Co-Channel Interferences in Asymmetric Channels. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0810114-134143

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Kung-hsuan. “Performance Analysis of Dual-hop Selective Relaying Scheme with Co-Channel Interferences in Asymmetric Channels.” 2014. Thesis, NSYSU. Accessed March 06, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0810114-134143.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Kung-hsuan. “Performance Analysis of Dual-hop Selective Relaying Scheme with Co-Channel Interferences in Asymmetric Channels.” 2014. Web. 06 Mar 2021.

Vancouver:

Lin K. Performance Analysis of Dual-hop Selective Relaying Scheme with Co-Channel Interferences in Asymmetric Channels. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Mar 06]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0810114-134143.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin K. Performance Analysis of Dual-hop Selective Relaying Scheme with Co-Channel Interferences in Asymmetric Channels. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0810114-134143

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

22. Qawasmeh, Yasmeen. Zweidimensionale potentielle Energieoberflächen der Bindung von CO / NO mit Münzmetallen.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-27249

► Two-dimensional potential energy surfaces of charged and neutral MXO (X: C, N and M: Au, Ag, Cu) and neutral M2XO complexes were calculated. Our results… (more)

Subjects/Keywords: Coupled Cluster singles doubles perturbative triples CCSD(T); coinage metals; carbon monoxide CO; nitrogen monoxide NO; Potential energy surface PES; ddc:541

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qawasmeh, Y. (2020). Zweidimensionale potentielle Energieoberflächen der Bindung von CO / NO mit Münzmetallen. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27249

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Qawasmeh, Yasmeen. “Zweidimensionale potentielle Energieoberflächen der Bindung von CO / NO mit Münzmetallen.” 2020. Thesis, Freie Universität Berlin. Accessed March 06, 2021. http://dx.doi.org/10.17169/refubium-27249.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Qawasmeh, Yasmeen. “Zweidimensionale potentielle Energieoberflächen der Bindung von CO / NO mit Münzmetallen.” 2020. Web. 06 Mar 2021.

Vancouver:

Qawasmeh Y. Zweidimensionale potentielle Energieoberflächen der Bindung von CO / NO mit Münzmetallen. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Mar 06]. Available from: http://dx.doi.org/10.17169/refubium-27249.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Qawasmeh Y. Zweidimensionale potentielle Energieoberflächen der Bindung von CO / NO mit Münzmetallen. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27249

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

23. Weber, Jan Phillip. Generierung, Aufrechterhaltung und Schicksal Follikulärer T-Helferzellen.

Degree: 2013, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-4311

► Humorale Immunreaktionen werden durch Antikörper-sezernierende B-Zellen vermittelt, die die Grundlage heutiger Impfstrategien sind. Aktivierte B-Zellen reifen in Keimzentren und abnormale Keimzentren werden mit vielen Autoimmunerkrankungen… (more)

▼ Humorale Immunreaktionen werden durch Antikörper-sezernierende B-Zellen vermittelt, die die Grundlage heutiger Impfstrategien sind. Aktivierte B-Zellen reifen in Keimzentren und abnormale Keimzentren werden mit vielen Autoimmunerkrankungen in Verbindung gebracht. Follikuläre T-Helferzellen (TFH) sind die CD4+ T-Helferzellen, die B-Zellen in der Keimzentrumsreaktion unterstützen. Um bessere Impfstoffe und therapeutische Ansätze gegen Autoimmunerkrankungen entwickeln zu können, ist es notwendig, die Differenzierung und Aufrechterhaltung von TFH-Zellen besser zu verstehen. Zugrundeliegende molekulare Mechanismen wurden in dieser Arbeit in einem adoptiven Transfersystem analysiert. Die Kostimulatoren ICOS und CD28 sind wichtig für TFH-Zellen, allerdings ist ihre spezielle Rolle für diese Zellen bisher nicht bekannt. Die hier präsentierten Daten weisen jedem der Moleküle eine exklusive Rolle in verschiedenen Stadien der humoralen Immunreaktion zu: CD28 regulierte die frühe Expression des TFH-Mastertranskriptionsfaktors Bcl-6, wohingegen ICOS-Kostimulation für die Aufrechterhaltung des TFH- Phänotyps und daher auch der Keimzentrumsreaktionen nötig war. Es war lange offen, ob TFH-Zellen nach Beendigung der Keimzentrumsreaktion zu CD4+ Gedächtniszellen werden. Um diese Frage zu beantworten, wurde ein Retransfersystem für TFH-Zellen entwickelt, in dem antigenspezifische TFH- und nicht-TFH-Effektorzellen aus Keimzentrumsreaktionen isoliert und sortiert wurden. Anschließend wurden die Zellen in naive Mäuse transferiert, wo sie unabhängig voneinander analysiert werden konnten. Die retransferierten Zellpopulationen kontrahierten schnell ohne Antigen, jedoch blieb eine kleine Population TFH- und nicht-TFH-Gedächtniszellen für mindestens vier Wochen in der Maus vorhanden. TFH-Zellen regulierten für ihre Identifikation und Funktion typische Moleküle herunter, behielten jedoch einen anderen Phänotyp als nicht-TFH-Gedächtniszellen. Nach Antigenstimulation in vivo adaptierten ehemalige TFH-Zellen präferentiell den TFH-Phänotyp. Ein erhöhtes Potential IL-21 zu sezernieren, gepaart mit erhöhter Expression von CXCR5 und geringerer Expression von CCR7, sollte diese Zellen zu effektiven B-Zell-Helfern in sekundären Immunreaktionen machen. Meine Arbeit zeigt funktionelle Unterschiede der strukturell verwandten Moleküle CD28 und ICOS auf und könnte daher ein Mosaikstein für die Suche nach besseren therapeutischen Ansätzen zur Bekämpfung von Autoimmunkrankheiten sein. Die Daten zeigen weiterhin, dass TFH-Zellen zu Gedächtniszellen werden können, welche ein interessanter Schwerpunkt zukünftiger Impfstrategien sein können. Advisors/Committee Members: [email protected] (contact), m (gender), Dr. Andreas Hutloff (firstReferee), Prof. Dr. Rupert Mutzel (furtherReferee).

Subjects/Keywords: immunology; co-stimulation; ICOS; CD28; T Follicular Helper Cells; TFH; GC; Germinal Center; memory; CTLA4-Ig; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weber, J. P. (2013). Generierung, Aufrechterhaltung und Schicksal Follikulärer T-Helferzellen. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-4311

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Weber, Jan Phillip. “Generierung, Aufrechterhaltung und Schicksal Follikulärer T-Helferzellen.” 2013. Thesis, Freie Universität Berlin. Accessed March 06, 2021. http://dx.doi.org/10.17169/refubium-4311.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Weber, Jan Phillip. “Generierung, Aufrechterhaltung und Schicksal Follikulärer T-Helferzellen.” 2013. Web. 06 Mar 2021.

Vancouver:

Weber JP. Generierung, Aufrechterhaltung und Schicksal Follikulärer T-Helferzellen. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2021 Mar 06]. Available from: http://dx.doi.org/10.17169/refubium-4311.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Weber JP. Generierung, Aufrechterhaltung und Schicksal Follikulärer T-Helferzellen. [Thesis]. Freie Universität Berlin; 2013. Available from: http://dx.doi.org/10.17169/refubium-4311

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

24. Bock, Cristin Nadine. Identifizierung und Charakterisierung muriner und humaner Th2/1-Hybridzellen in Th2-gesteuerten Erkrankungen.

Degree: 2019, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-25660

► CD4+ T-Zellen lassen sich in verschiedene Subpopulationen unterteilen, die aufgrund ihrer produzierten Zytokine unterschiedliche Effektorfunktionen erfüllen. Frühere Studien haben gezeigt, dass einige Populationen von T-Helferzellen… (more)

▼ CD4+ T-Zellen lassen sich in verschiedene Subpopulationen unterteilen, die aufgrund ihrer produzierten Zytokine unterschiedliche Effektorfunktionen erfüllen. Frühere Studien haben gezeigt, dass einige Populationen von T-Helferzellen (Th) Eigenschaften zweier gegensätzlicher Differenzierungsprogramme aufweisen und damit einen gemischten, so genannten „Hybrid“-Phänotyp darstellen. Mäuse entwickelten als Teil der Immunantwort auf Th2-polarisierende Wurminfektionen Th2/1-Hybridzellen, welche die Th2- und Th1-Linien-spezifischen Transkriptionsfaktoren GATA-3 sowie T-bet co-exprimieren. Dies ermöglicht den Th2/1-Zellen die Co-Produktion von Th2-(IL-4/-5/-13) und Th1-Zytokinen (IFN-γ). Dieser Th2/1-Phänotyp blieb über Monate hinweg stabil und löste im Vergleich zu konventionellen Th2- und Th1-Zellen eine geringere Immunpathologie in murinen Typ-1 und Typ-2-Entzündungsmodellen aus. Basierend auf diesen Daten war das erste Ziel dieser Arbeit Th2- und Th2/1- Zellen am Beispiel von Zwergfadenwurminfektionen (Strongyloidiasis) zu detektieren und phänotypisch zu charakterisieren. Dafür wurden T-Zellen aus Organen von Mäusen und periphere Blutproben indischer Patienten, die mit Zwergfadenwürmern infiziert waren, mittels durchflußzytometrischer Analysen untersucht. Das zweite Ziel war die Existenz des hybriden Phänotyps bei einer nicht-parasitären Th2-vermittelten entzündlichen Hauterkrankung, der atopischen Dermatitis (AD), nachzuweisen. Hierfür wurden Blutproben europäischer AD-Patienten hinsichtlich Th2/1-Zellen untersucht und überprüft, ob deren Zellfrequenzen mit der Schwere der Erkrankung assoziiert sind. Zusätzlich wurden die pro-inflammatorischen Th2- sowie Th22-Populationen und regulatorische Foxp3+ T-Zellen untersucht. In einem weiteren Teil dieser Arbeit wurden die Effektorfunktionen humaner Th2/1-Zellen in einem 3D-Hautmodell für AD analysiert. Die vorliegende Arbeit zeigt, dass die Bildung von Th2/1-Zellen in Mäusen ebenfalls durch Fadenwürmer induziert wird. Dabei hatten weder der Untersuchungszeitpunkt noch Unterschiede in der Parasitenlast einen Einfluss auf die Th2/1-Zellfrequenzen. Die untersuchten Organe unterschieden sich jedoch deutlich im Th2/1-Hybridanteil. Darüber hinaus konnten in dieser Arbeit erstmalig humane Th2/1-Zellen nachgewiesen werden, die ähnlich wie murine Hybride Th2- und Th1-Zytokine co-exprimieren. Eine vergleichende Analyse des murinen und humanen Th2/1-Phänotyps zeigte Ähnlichkeiten, wie die gegenüber Th2-Zellen verminderte Th2-Zytokinproduktion. Es wurden aber auch artspezifische Unterschiede, wie die stärkere IFN-γ Produktion durch humane Th2/1-Zellen, festgestellt. Die Analyse von Blutproben atopischer Patienten ergab, dass der Mehrheit der zirkulierenden Th2/1-Zellen der Rezeptor CLA fehlt, der für das Einwandern in die Haut benötigt wird. Somit stellt der Großteil der Th2/1-Zellen in AD-Patienten vermutlich keine hautinfiltrierende Zellpopulation dar. Im Gegensatz dazu waren pro-inflammatorische Th2- und Th22-Zellen vor allem in Patienten mit stark ausgeprägter AD in… Advisors/Committee Members: female (gender), Hartmann, Susanne (firstReferee), Rolff, Jens (furtherReferee).

Subjects/Keywords: Th2; GATA-3/T-bet; co-expression; cytokines; nematode infection; strongyloides; atopic dermatitis; skin equivalent; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bock, C. N. (2019). Identifizierung und Charakterisierung muriner und humaner Th2/1-Hybridzellen in Th2-gesteuerten Erkrankungen. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-25660

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bock, Cristin Nadine. “Identifizierung und Charakterisierung muriner und humaner Th2/1-Hybridzellen in Th2-gesteuerten Erkrankungen.” 2019. Thesis, Freie Universität Berlin. Accessed March 06, 2021. http://dx.doi.org/10.17169/refubium-25660.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bock, Cristin Nadine. “Identifizierung und Charakterisierung muriner und humaner Th2/1-Hybridzellen in Th2-gesteuerten Erkrankungen.” 2019. Web. 06 Mar 2021.

Vancouver:

Bock CN. Identifizierung und Charakterisierung muriner und humaner Th2/1-Hybridzellen in Th2-gesteuerten Erkrankungen. [Internet] [Thesis]. Freie Universität Berlin; 2019. [cited 2021 Mar 06]. Available from: http://dx.doi.org/10.17169/refubium-25660.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bock CN. Identifizierung und Charakterisierung muriner und humaner Th2/1-Hybridzellen in Th2-gesteuerten Erkrankungen. [Thesis]. Freie Universität Berlin; 2019. Available from: http://dx.doi.org/10.17169/refubium-25660

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. HARIS. FUNCTIONAL CHARACTERIZATION AND MECHANISTIC STUDIES OF A FUNGAL IMMUNOMODULATORY PROTEIN-FVE ON MURINE T CELLS.

Degree: 2015, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/120523

Subjects/Keywords: FIP-fve; Immunomodulation; Adjuvant; CD8+ T cell; Co-stimulatory molecules; CD27

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

HARIS. (2015). FUNCTIONAL CHARACTERIZATION AND MECHANISTIC STUDIES OF A FUNGAL IMMUNOMODULATORY PROTEIN-FVE ON MURINE T CELLS. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/120523

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

HARIS. “FUNCTIONAL CHARACTERIZATION AND MECHANISTIC STUDIES OF A FUNGAL IMMUNOMODULATORY PROTEIN-FVE ON MURINE T CELLS.” 2015. Thesis, National University of Singapore. Accessed March 06, 2021. http://scholarbank.nus.edu.sg/handle/10635/120523.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

HARIS. “FUNCTIONAL CHARACTERIZATION AND MECHANISTIC STUDIES OF A FUNGAL IMMUNOMODULATORY PROTEIN-FVE ON MURINE T CELLS.” 2015. Web. 06 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

HARIS. FUNCTIONAL CHARACTERIZATION AND MECHANISTIC STUDIES OF A FUNGAL IMMUNOMODULATORY PROTEIN-FVE ON MURINE T CELLS. [Internet] [Thesis]. National University of Singapore; 2015. [cited 2021 Mar 06]. Available from: http://scholarbank.nus.edu.sg/handle/10635/120523.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

HARIS. FUNCTIONAL CHARACTERIZATION AND MECHANISTIC STUDIES OF A FUNGAL IMMUNOMODULATORY PROTEIN-FVE ON MURINE T CELLS. [Thesis]. National University of Singapore; 2015. Available from: http://scholarbank.nus.edu.sg/handle/10635/120523

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

26. CECCARELLO ERICA. ENGINEERING T LYMPHOCYTES WITH KNOWN SPECIFICITY AND MODIFIED FUNCTION FOR PERSONALIZED IMMUNE THERAPY.

Degree: 2019, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/156923

Subjects/Keywords: T lymphocytes; Antisense Oligonucleotides; co-electroporation; soluble PD-1; tumor microenvironment; functional modification

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ERICA, C. (2019). ENGINEERING T LYMPHOCYTES WITH KNOWN SPECIFICITY AND MODIFIED FUNCTION FOR PERSONALIZED IMMUNE THERAPY. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/156923

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ERICA, CECCARELLO. “ENGINEERING T LYMPHOCYTES WITH KNOWN SPECIFICITY AND MODIFIED FUNCTION FOR PERSONALIZED IMMUNE THERAPY.” 2019. Thesis, National University of Singapore. Accessed March 06, 2021. https://scholarbank.nus.edu.sg/handle/10635/156923.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ERICA, CECCARELLO. “ENGINEERING T LYMPHOCYTES WITH KNOWN SPECIFICITY AND MODIFIED FUNCTION FOR PERSONALIZED IMMUNE THERAPY.” 2019. Web. 06 Mar 2021.

Vancouver:

ERICA C. ENGINEERING T LYMPHOCYTES WITH KNOWN SPECIFICITY AND MODIFIED FUNCTION FOR PERSONALIZED IMMUNE THERAPY. [Internet] [Thesis]. National University of Singapore; 2019. [cited 2021 Mar 06]. Available from: https://scholarbank.nus.edu.sg/handle/10635/156923.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ERICA C. ENGINEERING T LYMPHOCYTES WITH KNOWN SPECIFICITY AND MODIFIED FUNCTION FOR PERSONALIZED IMMUNE THERAPY. [Thesis]. National University of Singapore; 2019. Available from: https://scholarbank.nus.edu.sg/handle/10635/156923

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Swedish University of Agricultural Sciences

27. Liu, Tong. Biogas production from lignocellulosic agricultural residues.

Degree: 2019, Swedish University of Agricultural Sciences

URL: https://pub.epsilon.slu.se/15820/

► Methane, produced through microbial anaerobic digestion of various organic materials, is seen as a promising sustainable bioenergy source with the potential to reduce the current… (more)

▼ Methane, produced through microbial anaerobic digestion of various organic materials, is seen as a promising sustainable bioenergy source with the potential to reduce the current dependence on fossil fuels. Among organic materials, lignocellulosic materials, especially agriculture residues, are highly interesting due to high abundance and potential for methane production. However, low nutrient content and highly recalcitrant structure often limit process efficiency. This thesis presents the results of in-depth studies conducted in order to obtain new information about lignocellulose-degrading bacteria in biogas processes and to identify ways to enable more efficient biogas production. Different biogas processes were investigated in terms of their overall microbial community (bacteria and archaea) and potential lignocellulose degraders. The results showed that the biogas processes differed with regard to overall microbial community and chemical composition, but also composition of the cellulose-degrading bacterial community. These differences significantly influenced the degradation efficiency of both cellulose and wheat straw in batch digestion systems and also performance during start-up of semi-continuous stirred tank reactor (CSTR) processes. A positive correlation was found between lignocellulose degradation efficiency and relative abundance of Clostridium cellulolyticum. Ammonia level in the inoculum was identified as the most significant factor potentially affecting microbial community structure and methane production from lignocellulosic materials. Microbial and chemical composition of the original inoculum sources also influenced long-term degradation of lignocellulose in CSTR and appeared to influence residual methane potential. Different molecular methods for microbial community analysis were explored, with the aim of building an appropriate pipeline for in-depth studies of lignocellulose degraders in anaerobic reactors. This thesis provides novel information about the microbial communities involved in degradation of lignocellulosic materials and possible connections to process parameters. This information could potentially enable biogas production to be steered towards a more efficient and controllable process for degradation and biogas production from agriculture residues and plant-based materials.

Subjects/Keywords: anaerobic digestion; lignocellulose; hydrolase families 5 and 48; biomethane potential; continuous stirred-tank reactor; co-digestion; residual methane potential; next-generation amplicon sequencing; terminal restriction fragment length polymorphism (T-RFLP)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, T. (2019). Biogas production from lignocellulosic agricultural residues. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from https://pub.epsilon.slu.se/15820/

Chicago Manual of Style (16^th Edition):

Liu, Tong. “Biogas production from lignocellulosic agricultural residues.” 2019. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed March 06, 2021. https://pub.epsilon.slu.se/15820/.

MLA Handbook (7^th Edition):

Liu, Tong. “Biogas production from lignocellulosic agricultural residues.” 2019. Web. 06 Mar 2021.

Vancouver:

Liu T. Biogas production from lignocellulosic agricultural residues. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2019. [cited 2021 Mar 06]. Available from: https://pub.epsilon.slu.se/15820/.

Council of Science Editors:

Liu T. Biogas production from lignocellulosic agricultural residues. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2019. Available from: https://pub.epsilon.slu.se/15820/

The Ohio State University

28. Li, Ou. CD24 in T lymphocyte homeostatic proliferation and autoimmune disease.

Degree: PhD, Pathology, 2005, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1124255377

► In this thesis, we show that CD24 can regulate T cell homeostatic proliferation and autoimmune disease. The activation of T lymphocyte requires two signals from… (more)

▼ In this thesis, we show that CD24 can regulate T cell homeostatic proliferation and autoimmune disease. The activation of T lymphocyte requires two signals from antigen presenting cells, peptide/self-MHC complex to TCR and co-stimulatory signals. CD24, a glycosyl-phosphatidylinositol (GPI) arched glycoprotein, is an important co-stimulatory molecule. Although it is not essential for induction of T cell response, CD24 is required to express on T cells and for the induction of experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS). Here, we show that CD24 is essential for local clonal expansion and persistence of T cells after their migration into the CNS, and that expression of CD24 on either hematopoietic cells or non-hematopoietic antigen-presenting cells in the recipient is sufficient to confer susceptibility to EAE. At the same time, it is well established that T lymphocytes undergo homeostatic proliferation in lymphopenic environment. Recent studies have demonstrated that co-stimulation, mediated by CD28, 4-1BB, and CD40, is not required for T cell homeostatic proliferation. Here, we report that T cells from mice with a targeted mutation of CD24 have a remarkably reduced rate of proliferation when adoptively transferred into syngeneic lymphopenic hosts. The reduced proliferation cannot be attributed to abnormal survival and homing properties of the CD24-deficient T cells. Thus, although distinct co-stimulatory molecules are involved in antigen-driven proliferation and homeostatic proliferation, both processes can be modulated by co-stimulatory molecules. Surprisingly, in the lymphopenic CD24-deficient mice, T cells launch an uncontrollable proliferation that results in rapid death of the recipient mice. The dividing T cells have the phenotypes similar to those activated by cognate antigens. In addition, the CD24-deficient DC have superior capacity to drive homeostatic proliferation of the syngeneic cells. Thus, our data demonstrate that CD24 expressed on the host antigen-presenting cells limits T cell response to homeostatic cue and prevents fatal damage associated with the uncontrolled homeostatic proliferation. Taken together, in this thesis, we demonstrate that although CD24 is not essential for the induction of T cell response, it can regulate T cell homeostatic proliferation and its function in autoimmune disease. Advisors/Committee Members: Liu, Yang (Advisor).

Subjects/Keywords: CD24; co-stimulatory molecule; T cell Homeostatic proliferation; EAE; autoimmune disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, O. (2005). CD24 in T lymphocyte homeostatic proliferation and autoimmune disease. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1124255377

Chicago Manual of Style (16^th Edition):

Li, Ou. “CD24 in T lymphocyte homeostatic proliferation and autoimmune disease.” 2005. Doctoral Dissertation, The Ohio State University. Accessed March 06, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1124255377.

MLA Handbook (7^th Edition):

Li, Ou. “CD24 in T lymphocyte homeostatic proliferation and autoimmune disease.” 2005. Web. 06 Mar 2021.

Vancouver:

Li O. CD24 in T lymphocyte homeostatic proliferation and autoimmune disease. [Internet] [Doctoral dissertation]. The Ohio State University; 2005. [cited 2021 Mar 06]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1124255377.

Council of Science Editors:

Li O. CD24 in T lymphocyte homeostatic proliferation and autoimmune disease. [Doctoral Dissertation]. The Ohio State University; 2005. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1124255377

29. Cacere, Camila Rodrigues. Aspectos moleculares envolvidos na apoptose de células mononucleares em pacientes com paracoccidioidomicose.

Degree: PhD, Microbiologia, 2008, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19092008-095609/ ;

► A hiporreatividade das células T observada na resposta imune a antígenos de P. brasiliensis de pacientes com paracoccidioidomicose ativa deve contribuir para o não controle… (more)

▼ A hiporreatividade das células T observada na resposta imune a antígenos de P. brasiliensis de pacientes com paracoccidioidomicose ativa deve contribuir para o não controle da doença, levando à disseminação do fungo. É, na maioria das vezes, reversível com tratamento antifúngico. Os mecanismos que levam a esta hiporreatividade não são bem conhecidos. No entanto, foram demonstrados em resultados prévios em nosso laboratório que células mononucleares de pacientes frente a gp43 apresentam níveis elevados de apoptose. Para tentarmos explicar esse mecanismo, nossa primeira hipótese foi de avaliar se a ativação celular desses pacientes estavam sendo prejudicada por uma ativação inadequada induzida pela expressão alterada de moléculas co-estimulatórias como CD80, CD86, CD28, CD152, ICOS e PD-1. A expressão dessas moléculas foi avaliada em células T e monócitos de pacientes com doença ativa (n = 7...) e controles curados (n = 2...) de um episódio prévio de PCM, mantidas em cultura com antígeno metabólico de Candida albicans (CMA), gp43 ou sem estímulo após 4 dias em cultura. Os resultados obtidos demonstraram que a expressão do CD28 foi comparável entre doentes e controles, e que a expressão de CD152, PD-1 e ICOS, que preferencialmente exercem um papel negativo na sinalização celular, foi maior em células T de pacientes, estimuladas ou não, quando comparadas com células de indivíduos controles. Em paralelo, foram realizados experimentos com a adição dos respectivos anticorpos bloqueadores, que, no entanto, não restabeleceu a proliferação celular dos pacientes. A expressão das moléculas CD80 e CD86 na superfície dos monócitos foi similar em pacientes e controles. Já a expressão dessas moléculas na superfície de linfócitos foi maior nos pacientes tanto em células estimuladas como não estimuladas. O bloqueio com os respectivos anticorpos no dia 0 inibiu a resposta tanto com gp43 como com CMA, porém de forma diferenciada. Nos pacientes e controles a inibição da molécula CD86 diminuiu a resposta tanto para gp43 como para CMA e a inibição da molécula CD80 diminuiu a resposta proliferativa apenas para gp43, e somente no grupo controle, sugerindo que os diferentes antígenos exigem diferentes moléculas durante o processo de apresentação antigênica. A adição desses anticorpos no 4o dia da cultura não modificou a resposta linfoproliferantiva dos pacientes e controles. Nossos dados favorecem a hipótese, derivada de outros modelos de exposição crônica a antígenos exógenos, de que a exposição repetida a antígenos de P. brasiliensis por um longo período in vivo, verificada nos pacientes com paracoccidioidomicose, levam as células T a um estado de tolerância adaptativa, que dificilmente é revertida in vitro. A partir desses resultados analisamos a participação da apoptose de células T nesse provável estado de tolerância nas células dos pacientes. Observamos que a expressão da molécula anti-apoptótica Bcl-2 está diminuída nas células T de pacientes previamente estimuladas comparadas com as células dos controles, e mesmo após o reestímulo… Advisors/Committee Members: Benard, Gil, Romano, Carla Cristina.

Subjects/Keywords: Paracoccidioides brasiliensis; Paracoccidioides brasiliensis; Adaptively tolerant T-cell; Antiapoptotic molecule; Costimulatory molecules; Expressão de caspase 8 e caspase 9; Expression of caspase 8 and caspase 9; Moléculas anti-apoptóticas; Moléculas co-stimulatórias; Paracoccidioidomicose; Paracoccidioidomycosis; Tolerância adaptativa de células T

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cacere, C. R. (2008). Aspectos moleculares envolvidos na apoptose de células mononucleares em pacientes com paracoccidioidomicose. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19092008-095609/ ;

Chicago Manual of Style (16^th Edition):

Cacere, Camila Rodrigues. “Aspectos moleculares envolvidos na apoptose de células mononucleares em pacientes com paracoccidioidomicose.” 2008. Doctoral Dissertation, University of São Paulo. Accessed March 06, 2021. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19092008-095609/ ;.

MLA Handbook (7^th Edition):

Cacere, Camila Rodrigues. “Aspectos moleculares envolvidos na apoptose de células mononucleares em pacientes com paracoccidioidomicose.” 2008. Web. 06 Mar 2021.

Vancouver:

Cacere CR. Aspectos moleculares envolvidos na apoptose de células mononucleares em pacientes com paracoccidioidomicose. [Internet] [Doctoral dissertation]. University of São Paulo; 2008. [cited 2021 Mar 06]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19092008-095609/ ;.

Council of Science Editors:

Cacere CR. Aspectos moleculares envolvidos na apoptose de células mononucleares em pacientes com paracoccidioidomicose. [Doctoral Dissertation]. University of São Paulo; 2008. Available from: http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19092008-095609/ ;

Universidade de Lisboa

30. Santos, Andreia Sofia Ramalho dos. Role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.

Degree: 2016, Universidade de Lisboa

URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/25115

►

Tese de mestrado em Biologia Molecular e Genética, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2016

Os progenitores de células T sofrem… (more)

▼

Tese de mestrado em Biologia Molecular e Genética, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2016

Os progenitores de células T sofrem uma série de transformações durante o seu desenvolvimento incluindo seleção, comprometimento para uma determinada linhagem (CD8 ou CD4) e maturação no timo de modo a poderem diferenciar-se em células T funcionais CD4 auxiliares ou CD8 citotóxicas. A decisão de se diferenciar numa das duas linhagens envolve o recetor de células T (TCR), sinalização por parte de interleucinas (IL) e fatores de transcrição (TF) específicos para cada linhagem, crendo-se, atualmente, ser um processo vitualmente irreversível. Compreender as bases que fundamentam a decisão de uma célula se diferenciar numa determinada linhagem é de extrema importância para a compreensão dos mecanismos envolvidos no desenvolvimento dos timócitos humanos. Deste modo, este modelo é dos mais debatidos em imunologia apesar de se recorrer maioritariamente ao modelo animal do ratinho que, apesar de ter proporcionado um vasto leque de conhecimentos, nem sempre se aplicam ao modelo humano, raramente estudado. O modelo de sinalização cinética definido em ratinho para explicar o desenvolvimento dos timócitos aponta para a IL-7 como força motriz na especificação e diferenciação em células CD8, sendo a sua ausência durante o desenvolvimento no timo responsável pela manutenção do fenótipo CD4 definido nos estadios mais precoces de maturação. A IL-4 tinha já sido testada no modelo do ratinho tendo tido fraco desempenho no sentido de conduzir à diferenciação dos timócitos em células T CD8 quando comparada com a IL-7. A IL-4, membro da família de recetores com cadeia γ, foi originalmente descrita em 1982 como sendo uma citocina pleiotrópica de tipo I produzida maioritariamente por linfócitos T auxiliares do tipo 2 (Th2), basófilos, eosinófilos, mastócitos, células natural killer (NK), células T foliculares (Tfh) e células T γ/δ. Esta citocina tem a função de induzir a polarização do fenótipo Th2 e suprimir a diferenciação das células Th1. Adicionalmente, a IL-4 promove o crescimento e diferenciação dos linfócitos B, controlando a especificidade das alterações de classe de imunoglobulinas tal como o desenvolvimento de células B de memória. As funções fisiológicas desta citocina são mediadas pelo recetor de IL-4, composto por uma cadeia α de 140kDa e uma cadeia γ partilhada com os recetores das citocinas IL-2, IL-7, IL-9 e IL-15. Este dímero pode ser encontrado em células hematopoiéticas ativadas exclusivamente após ligação da IL-4. Após ligação da IL-4 ao seu recetor é promovida ativação do ativador de transcrição e transdutor de sinal 6 (STAT6), que por sua vez promove a regulação de genes responsáveis pela diferenciação de células T CD4 ativadas em células Th2 e Th9, expressão de GATA binding protein 3 (GATA3), produção de quimiocinas e pelo aumento da expressão da imunoglubina IgE responsável pelo desenvolvimento de uma resposta imunológica alérgica. Neste projeto sugerimos um papel para a IL-4 na…

Advisors/Committee Members: Cabaço, Helena Isabel Marques Nunes,1979-, Telhada, Maria Margarida Blasques,1951-.

Subjects/Keywords: Desenvolvimento e diferenciação das células T humanas; Comprometimento das células T para uma determinada linhagem; IL-4; Plasticidade dos timócitos CD4SP; Expressão dos co-recetores CD8a e CD8B; Teses de mestrado - 2016; Domínio/Área Científica::Ciências Naturais::Ciências Biológicas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santos, A. S. R. d. (2016). Role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes. (Thesis). Universidade de Lisboa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/25115

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santos, Andreia Sofia Ramalho dos. “Role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.” 2016. Thesis, Universidade de Lisboa. Accessed March 06, 2021. http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/25115.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santos, Andreia Sofia Ramalho dos. “Role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes.” 2016. Web. 06 Mar 2021.

Vancouver:

Santos ASRd. Role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes. [Internet] [Thesis]. Universidade de Lisboa; 2016. [cited 2021 Mar 06]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/25115.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santos ASRd. Role of IL-4 in the lineage plasticity of human CD4 single positive thymocytes. [Thesis]. Universidade de Lisboa; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/25115

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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