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You searched for subject:(Chromatin physiology 60). Showing records 1 – 2 of 2 total matches.

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1. Joo, Heui Yun. Understanding the regulatory mechanisms of UBP-M and H2A deubiquitination in chromatin and cellular functions.

Degree: PhD, 2009, University of Alabama – Birmingham

Posttranslational modifications of histones regulate important chromatin and cellular functions. Among them, ubiquitination of histone H2A is correlated to transcriptional repression, such as HOX gene silencing and X chromosome inactiviation. Little was known about the removal of ubiquitin from histones, the enzyme(s) involved and its function in chromatin dynamics. We have identified the protein Ubp-M (USP16) to be the H2A- and nucleosome-specific deubiquitinase. We also demonstrated that Ubp- M-mediated H2A deubiquitination is involved in cell cycle progression to M-phase, HOX gene expression, and posterior development in Xenopus laevis. Furthermore, we have also purified USP12 and USP46 which contain an Ubp-M independent deubiquitinase activity for both uH2A and uH2B. USP12 and USP46 each form a complex with the WD40 repeat-containing protein WDR48, which is required for the deubiquitinase activity. USP12 and USP46 regulate HOX gene expression and gastrulation during Xenopus laevis development. These studies will contribute to the understanding of the regulatory mechanisms of H2A and H2B ubiquitination and deubiquitination, and their biological functions.

1 online resource (x, 90 p.) : ill., digital, PDF file.

Biochemistry and Molecular Genetics;

Joint Health Sciences;

histone H2A ubiquitination deubiquitination Ubp-M

UNRESTRICTED

Advisors/Committee Members: Wang, Hengbin, Chang, Chenbei<br>, Chow, Louise<br>, Ruppert, J. Michael<br>, Townes, Tim M..

Subjects/Keywords: Chromatin  – physiology<; br>; Endopeptidases  – metabolism<; br>; Histones  – metabolism<; br>; Ubiquitin Thiolesterase  – metabolism<; br>; Xenopus Proteins  – metabolism<; br>; Xenopus laevis  – embryology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Joo, H. Y. (2009). Understanding the regulatory mechanisms of UBP-M and H2A deubiquitination in chromatin and cellular functions. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,1101

Chicago Manual of Style (16th Edition):

Joo, Heui Yun. “Understanding the regulatory mechanisms of UBP-M and H2A deubiquitination in chromatin and cellular functions.” 2009. Doctoral Dissertation, University of Alabama – Birmingham. Accessed April 04, 2020. http://contentdm.mhsl.uab.edu/u?/etd,1101.

MLA Handbook (7th Edition):

Joo, Heui Yun. “Understanding the regulatory mechanisms of UBP-M and H2A deubiquitination in chromatin and cellular functions.” 2009. Web. 04 Apr 2020.

Vancouver:

Joo HY. Understanding the regulatory mechanisms of UBP-M and H2A deubiquitination in chromatin and cellular functions. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2009. [cited 2020 Apr 04]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,1101.

Council of Science Editors:

Joo HY. Understanding the regulatory mechanisms of UBP-M and H2A deubiquitination in chromatin and cellular functions. [Doctoral Dissertation]. University of Alabama – Birmingham; 2009. Available from: http://contentdm.mhsl.uab.edu/u?/etd,1101

2. Kim, Junghyun. Transcriptional regulation of the human heme oxygenase-1 via chromatin looping in renal cells.

Degree: PhD, 2010, University of Alabama – Birmingham

Heme oxygenase-1 (HO-1) is a critical enzyme catalyzing the degradation of heme and generating carbon monoxide, iron, and biliverdin. In addition to heme degradation, HO-1 expression is known to protect against various cellular insults and disease states including acute kidney injury, atherosclerosis, vascular restenosis, and others. Human HO-1 gene expression is tightly regulated at the level of transcription. The main goal of this study is to explore the transcriptional regulation of the human HO-1 gene in renal epithelial cells. Chromosome Conformation Capture (3C) demonstrates that multiple regulatory regions within the HO-1 promoter and enhancer regions are physically interacting with each other by forming chromatin loops and this looping is required for the initiation of the human HO-1 gene transcription. ChIP-Loop assay reveals that these regions share several common transcription factors such as Sp1, USF-1, and JunB. Sp1 binds to the intronic enhancer which interacts with the -4.5kb promoter region by chromatin looping. Inhibition of Sp1 by siRNA abolishes this interaction and reduces HO-1 transcription. To study human HO-1 transcription in vivo, a HO-1 bacterial artificial chromosome (BAC) transgenic mouse was generated by integrating a 87 kb BAC DNA (a portion of the human chromosome 22 including HO-1 gene and its regulatory regions) into the mouse genome. Global overexpression of HO-1 mRNA and protein in tissues of HO-1 BAC transgenic mice were detected. Moreover, using this HO- 1 BAC transgenic mouse, a “humanized” HO-1 BAC transgenic mouse (hHO-1 BAC) was generated by crossing HO-1 BAC mice with HO-1 knockout (HO-1-/-) mice. In addition to the overexpression of human-specific HO-1 mRNA and protein expression, the human HO-1 gene in hHO-1 BAC mice rescued the abnormal phenotype observed in the HO-1-/- mice such as increased incidence of abortion and embryonic lethality, heightened sensitivity to acute kidney injury, iron overload, anemia, and splenomegaly. These studies provide not only functional insights for the regulation of the human HO-1 gene but also the molecular architecture of the human chromosome containing the HO-1 gene and promoter in renal epithelial cells.

1 online resource (xiv, 136 p.) : ill., digital, PDF file.

Joint Health Sciences;

Heme oxygenase-1 Chromatin looping Transgenic mice Acute kidney injury rhabdomyolysis chromosome conformation capture

UNRESTRICTED

Advisors/Committee Members: McDonald, Jay M., Agarwal, Anupam<br>, Chugh, Sumant S.<br>, Hardy, Robert W.<br>, Ponnazhagan, Selvaragan<br>, Ryan, Thomas.

Subjects/Keywords: Acute Kidney Injury<; br>; Chromatin  – chemistry<; br>; Gene Expression Regulation, Enzymologic<; br>; Heme Oxygenase-1  – biosynthesis<; br>; Kidney  – enzymology<; br>; Mice<; br>; Sp1 Transcription Factor  – physiology

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kim, J. (2010). Transcriptional regulation of the human heme oxygenase-1 via chromatin looping in renal cells. (Doctoral Dissertation). University of Alabama – Birmingham. Retrieved from http://contentdm.mhsl.uab.edu/u?/etd,1100

Chicago Manual of Style (16th Edition):

Kim, Junghyun. “Transcriptional regulation of the human heme oxygenase-1 via chromatin looping in renal cells.” 2010. Doctoral Dissertation, University of Alabama – Birmingham. Accessed April 04, 2020. http://contentdm.mhsl.uab.edu/u?/etd,1100.

MLA Handbook (7th Edition):

Kim, Junghyun. “Transcriptional regulation of the human heme oxygenase-1 via chromatin looping in renal cells.” 2010. Web. 04 Apr 2020.

Vancouver:

Kim J. Transcriptional regulation of the human heme oxygenase-1 via chromatin looping in renal cells. [Internet] [Doctoral dissertation]. University of Alabama – Birmingham; 2010. [cited 2020 Apr 04]. Available from: http://contentdm.mhsl.uab.edu/u?/etd,1100.

Council of Science Editors:

Kim J. Transcriptional regulation of the human heme oxygenase-1 via chromatin looping in renal cells. [Doctoral Dissertation]. University of Alabama – Birmingham; 2010. Available from: http://contentdm.mhsl.uab.edu/u?/etd,1100

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