subject:(Chlamydomonas)

University of British Columbia

1. Butler, Gary Lee. Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae) .

Degree: 1970, University of British Columbia

► An investigation of an obligate psychrophilic green alga, Chlamydomonas sp., was undertaken to define some of its growth characteristics. It has a temperature optimum of… (more)

Subjects/Keywords: Chlamydomonas

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APA (6^th Edition):

Butler, G. L. (1970). Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae) . (Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/34567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Butler, Gary Lee. “Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae) .” 1970. Thesis, University of British Columbia. Accessed September 16, 2019. http://hdl.handle.net/2429/34567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Butler, Gary Lee. “Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae) .” 1970. Web. 16 Sep 2019.

Vancouver:

Butler GL. Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae) . [Internet] [Thesis]. University of British Columbia; 1970. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/2429/34567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Butler GL. Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae) . [Thesis]. University of British Columbia; 1970. Available from: http://hdl.handle.net/2429/34567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

2. Broderick, Lynne Sechrist. Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin.

Degree: PhD, Graduate School, 1969, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114

Subjects/Keywords: Biology; Chlamydomonas

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APA (6^th Edition):

Broderick, L. S. (1969). Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114

Chicago Manual of Style (16^th Edition):

Broderick, Lynne Sechrist. “Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin.” 1969. Doctoral Dissertation, The Ohio State University. Accessed September 16, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114.

MLA Handbook (7^th Edition):

Broderick, Lynne Sechrist. “Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin.” 1969. Web. 16 Sep 2019.

Vancouver:

Broderick LS. Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin. [Internet] [Doctoral dissertation]. The Ohio State University; 1969. [cited 2019 Sep 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114.

Council of Science Editors:

Broderick LS. Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin. [Doctoral Dissertation]. The Ohio State University; 1969. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114

Louisiana State University

3. Brumfield, Kristy Marie. Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis.

Degree: PhD, 2009, Louisiana State University

URL: etd-10022009-140919 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2296

► Ergosterol is the major sterol found in the membranes of Chlamydomonas reinhardtii. While past studies have identified some ergosterol mutants in C. reinhardtii, very little… (more)

Subjects/Keywords: lipid; sterol; Chlamydomonas reinhardtii; ergosterol

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APA (6^th Edition):

Brumfield, K. M. (2009). Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-10022009-140919 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2296

Chicago Manual of Style (16^th Edition):

Brumfield, Kristy Marie. “Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis.” 2009. Doctoral Dissertation, Louisiana State University. Accessed September 16, 2019. etd-10022009-140919 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2296.

MLA Handbook (7^th Edition):

Brumfield, Kristy Marie. “Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis.” 2009. Web. 16 Sep 2019.

Vancouver:

Brumfield KM. Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis. [Internet] [Doctoral dissertation]. Louisiana State University; 2009. [cited 2019 Sep 16]. Available from: etd-10022009-140919 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2296.

Council of Science Editors:

Brumfield KM. Investigations of Chlamydomonas reinhardtii ergosterol biosynthesis. [Doctoral Dissertation]. Louisiana State University; 2009. Available from: etd-10022009-140919 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2296

University of Iceland

4. Róbert Magnússon 1982. Greining á bakteríuhamlandi virkni hjá Chlamydomonas reinhardtii.

Degree: 2010, University of Iceland

URL: http://hdl.handle.net/1946/6343

►

Chlamydomonas reinhardtii er grænþörungur sem liggur nærri stofngreinum plantna og dýra. C. reinhardtii finnst um allan heim í ferskvatni, sjó og ýmsum jarðvegi. Hann er… (more)

▼

Chlamydomonas reinhardtii er grænþörungur sem liggur nærri stofngreinum plantna og dýra. C. reinhardtii finnst um allan heim í ferskvatni, sjó og ýmsum jarðvegi. Hann er einfruma, heilkjarna lífvera, u.þ.b. 10 µm í þvermál, syndir með tveimur svipum og hefur eitt grænukorn fyrir ljóstillífun. Chlamydomonas er notaður sem módel lífvera í rannsóknum á mörgum grundvallar spurningum í frumu- og sameindalíffræði. Þar sem hann lifir í umhverfi þar sem mikil bakteríu flóra þrífst er líklegt að hann hafi þróað með sér varnarkerfi lík þeim sem rannsökuð hafa verið hjá bæði dýrum og plöntum. Þessi varnarkerfi hafa ekki verið skilgreind en í ljós hefur komið að grænþörungurinn seytir bakteríuhamlandi efnum. Meðhöndlun með próteinösum bendir til að prótein eða peptíð standa að baki virkninni. Markmið þessa verkefnis var að hreinsa þessi bakteríuhamlandi efni og skilgreina með HPLC tækni (e. High Performance Liquid Chromatography) og massagreiningu. Tvö virk efni fundust, bæði næm fyrir próteinasa meltu með próteinasa K og pepsín. Tveir mismunandi hreinsunarferlar, samsettir af útdráttar súlum fyrir grófhreinsum og HPLC katjóna skipta-, öfugfasa- (e. reverse phase) og vatnsæknum súlum, voru hannaðir fyrir hreinsun og einangrun á þessum peptíðum/próteinum. Hreinsuð peptíð/prótein voru svo send á rannsóknarstofur erlendis til greiningar.

Chlamydomonas reinhardtii is a green algae found all over the world, in soil, fresh water and oceans. It is a unicellular, eukaryotic organism, about 10 µm in diameter and swims with two anterior flagella and contains a chloroplast for photosynthesis. Chlamydomonas is used as a model system for research on many fundamental question in cell and molecular biology, such as the function of photosynthesis and flagellar motility, organelle biogenesis, and genetics. Since Chlamydomonas lives in an environment filled with microbes, good and bad, it needs strong defense mechanisms to survive. Their defense systems have not been well defined but secreted antimicrobial activity was detected from Chlamydomonas. Goal of this project was to define this activity and analyse the molecules responsible for this activity using HPLC (high performance liquid chromatography) and mass spectrometry. Two active agents were found, both of them sensitive to protease digestion with proteinase K and pepsin. Two separate workflows were carryed out for cleaning and isolation for these peptides/proteins from secreted material from Chlamydomonas, using a combination of extraction columns and HPLC cation exchange-, reverse- phase and hydrophilic columns. Purified peptides/proteins were then send abroad for further analyzis.

Rannsóknasjóður (Rannís) Rannsóknasjóður Háskóla Íslands

Subjects/Keywords: Líffræði; Chlamydomonas reinhardtii; Grænþörungar

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APA (6^th Edition):

1982, R. M. (2010). Greining á bakteríuhamlandi virkni hjá Chlamydomonas reinhardtii. (Thesis). University of Iceland. Retrieved from http://hdl.handle.net/1946/6343

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

1982, Róbert Magnússon. “Greining á bakteríuhamlandi virkni hjá Chlamydomonas reinhardtii.” 2010. Thesis, University of Iceland. Accessed September 16, 2019. http://hdl.handle.net/1946/6343.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

1982, Róbert Magnússon. “Greining á bakteríuhamlandi virkni hjá Chlamydomonas reinhardtii.” 2010. Web. 16 Sep 2019.

Vancouver:

1982 RM. Greining á bakteríuhamlandi virkni hjá Chlamydomonas reinhardtii. [Internet] [Thesis]. University of Iceland; 2010. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1946/6343.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

1982 RM. Greining á bakteríuhamlandi virkni hjá Chlamydomonas reinhardtii. [Thesis]. University of Iceland; 2010. Available from: http://hdl.handle.net/1946/6343

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

5. Wolff, Darold Melvin, 1940-. Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii.

Degree: PhD, General Science, 1971, Oregon State University

URL: http://hdl.handle.net/1957/45431

► The primary objective of this study was to develop methods for the selection of temperature sensitive division (TSD) mutants of Chlamydomonas reinhardtii. Such methods were… (more)

▼ The primary objective of this study was to develop methods for the selection of temperature sensitive division (TSD) mutants of Chlamydomonas reinhardtii. Such methods were developed and four temperature sensitive (TS) mutants were isolated and partially characterized. Wild type (WT) cells of the plus mating type were synchronized by a 12 hr light-12 hr dark regime at 25 C in culture tubes containing a high salt minimal medium buffered with PIPES (Piperazine-N, N'- bis (2-ethane sulfonic acid) monosodium monohydrate). Cells were mutagenized by irradiation with 1725 ergs/mm² of 254 nm ultraviolet light (UV) during the first half of the light period (G₁). The irradiated cells were kept in the dark for 24 hr to prevent photoreactivation, and then treated with a 10 mM concentration of hydroxyurea (HU) for 24 hr at an elevated temperature of 33 C. It was determined that this concentration of HU and this duration of treatment effectively kills WT cells at some stage i.n the middle of the generation cycle (presumably S phase). Temperature sensitive mutants that cannot progress into the HU-sensitive phase at 33 C were expected to survive. The combined treatment with UV and FU reduced the population to approximately 1% and increased the yield of possible TSD mutants. (UV alone reduced the population to 7% and HU to about 6.9%.) The cells were then plated on agar plates made with minimal medium and returned to 33 C for 5 days. The colonies that appeared during this time were considered to have arisen from unmutated cells or non-TSD mutants that had somehow survived the selection treatment; these colonies were marked and the agar plates placed at 25 C for an additional 5 days. The colonies that now formers were considered possible TSD mutants. Of 116 such colonies, only four were found to be temperature sensitive after replating and testing them at 33 and 25 C, the permissive temperature in this study. The TS mutants isolated were partially characterized on the basis of their responses to the restrictive temperature. The mutants, along with the WT cells, were observed in microdrops of minimal or complete medium covered with paraffin oil and on minimal or complete agar plates. The percentages of cells having divided once, twice, and three or more times were recorded at various times during continuous incubation at 25 C and during and after a 24 hr exposure to 33 C. In addition, the mutants were observed for viability and morphological changes. The effects of light intensities of 0, 200, and 600 foot candles were also studied. It was concluded that no ideal TSD mutant had been isolated. An ideal TSD mutant would be one in which division could be reversibly blocked by a restrictive temperature at a specific stage of the generation cycle. Although such mutants were not isolated, three of the four TS mutants (TSLt-1, TSLt-2, and TSGL) appear to show responses at the 33 C which suggest that some specific phase of the generation cycle is impaired, but the effect is not reversible and the… Advisors/Committee Members: Nachtwey, Stuart (advisor), Hisaw, Frederick L. (committee member).

Subjects/Keywords: Chlamydomonas reinhardii

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APA (6^th Edition):

Wolff, Darold Melvin, 1. (1971). Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/45431

Chicago Manual of Style (16^th Edition):

Wolff, Darold Melvin, 1940-. “Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii.” 1971. Doctoral Dissertation, Oregon State University. Accessed September 16, 2019. http://hdl.handle.net/1957/45431.

MLA Handbook (7^th Edition):

Wolff, Darold Melvin, 1940-. “Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii.” 1971. Web. 16 Sep 2019.

Vancouver:

Wolff, Darold Melvin 1. Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Oregon State University; 1971. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1957/45431.

Council of Science Editors:

Wolff, Darold Melvin 1. Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Oregon State University; 1971. Available from: http://hdl.handle.net/1957/45431

University of Texas Southwestern Medical Center

6. Hernandez Lara, Carmen Isabel. Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1217

► The primary cilium is a signaling organelle found on most cell types in vertebrates. It is a distinct compartment for membrane and cytoplasmic proteins that… (more)

▼ The primary cilium is a signaling organelle found on most cell types in vertebrates. It is a distinct compartment for membrane and cytoplasmic proteins that mediate multiple sensory processes. Little is known about the cellular and molecular mechanisms that determine regulated ciliary localization of membrane or soluble proteins. Our laboratory uses the biflagellated green alga Chlamydomonas as a model system to study flagellar adhesion-induced signaling. During fertilization, Chlamydomonas mating type plus and mating type minus gametes undergo flagellar adhesion through the interaction of flagellar adhesion receptor activities (flagellar agglutinins) encoded by the SAG1 (plus gametes and SAD1 (minus gametes) genes, activating a cAMP-dependent signaling pathway that ultimately leads to cell fusion and zygote formation. Our lab recently developed a Chlamydomonas strain expressing a SAG1-HA transgene, making it possible to study directly the properties of the SAG1 gene product. We found that SAG1 is cleaved soon after its synthesis to generate a C-terminal 65kDa fragment (SAG1-HA-C65), which we showed is an integral membrane protein. In my studies here, I describe new findings on the properties of SAG1-HA-C65 and its trafficking into and from the flagellar membrane. I found that in resting gametes SAG1-HA-C65 was distributed over the entire surface of the cell body plasma membrane, with little in the flagellar membrane indicating that SAG1-HA-C65 was excluded from the flagellar membrane. After flagellar adhesion-induced gamete activation or after activation of gametes with di-butyryl cAMP, SAG1-HA-C65 rapidly became enriched in the flagellar membrane concomitant with becoming concentrated around the bases of the flagella at the apical end of the cell. Moreover, cytoplasmic microtubules were required for SAG1-HA-C65 apical accumulation and for its significant delivery to the flagella during signaling. Apical concentration and flagellar enrichment of SAG1-HA-C65 occurred in cells conditionally depleted of the anterograde intraflagellar transport motor protein, kinesin-2. Furthermore, studies on the fate of SAG1-HA-C65 showed that it was shed into the medium in a detergent soluble membrane fraction during flagellar adhesion. This work provides new insights into membrane protein trafficking that regulates the protein composition of the ciliary/flagellar membrane. Advisors/Committee Members: Snell, William J..

Subjects/Keywords: Protein Transport; Flagella; Chlamydomonas

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APA (6^th Edition):

Hernandez Lara, C. I. (2013). Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hernandez Lara, Carmen Isabel. “Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed September 16, 2019. http://hdl.handle.net/2152.5/1217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hernandez Lara, Carmen Isabel. “Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling.” 2013. Web. 16 Sep 2019.

Vancouver:

Hernandez Lara CI. Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/2152.5/1217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hernandez Lara CI. Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

7. Husic, Diane White. Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii.

Degree: PhD, Department of Biochemistry, 1986, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:24998

Subjects/Keywords: Chlamydomonas reinhardtii

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APA (6^th Edition):

Husic, D. W. (1986). Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:24998

Chicago Manual of Style (16^th Edition):

Husic, Diane White. “Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii.” 1986. Doctoral Dissertation, Michigan State University. Accessed September 16, 2019. http://etd.lib.msu.edu/islandora/object/etd:24998.

MLA Handbook (7^th Edition):

Husic, Diane White. “Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii.” 1986. Web. 16 Sep 2019.

Vancouver:

Husic DW. Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Michigan State University; 1986. [cited 2019 Sep 16]. Available from: http://etd.lib.msu.edu/islandora/object/etd:24998.

Council of Science Editors:

Husic DW. Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii. [Doctoral Dissertation]. Michigan State University; 1986. Available from: http://etd.lib.msu.edu/islandora/object/etd:24998

Universidad de Chile

8. Hun Arenas, Yasmín Carolina. Efecto de la concentración de la solución nutritiva sobre la biomasa de microalgas del género Chlamydomonas .

Degree: 2010, Universidad de Chile

URL: http://www.repositorio.uchile.cl/handle/2250/111421

► Las microalgas son capaces de producir diferentes compuestos de importancia nutricional, siendo utilizadas en acuicultura, alimentación animal y nutrición humana. Esta capacidad depende, entre otros… (more)

▼ Las microalgas son capaces de producir diferentes compuestos de importancia nutricional, siendo utilizadas en acuicultura, alimentación animal y nutrición humana. Esta capacidad depende, entre otros factores, de la concentración de nutrientes, lo que altera la composición bioquímica de las microalgas. Se evaluó el efecto de la concentración de la solución nutritiva de Hoagland y Arnon II modificada sobre la biomasa fresca, contenido de algunas biomoléculas de interés y sobre la capacidad antioxidante de microalgas Chlamydomonas, género que es utilizado como sistema modelo de investigación en biología celular pero que hasta el momento no ha sido estudiado desde el punto de vista nutricional ni desde sus potencialidades comerciales. Mediante la combinación de dos métodos de aislamiento: rayado en agar y dilución seriada, se logró aislar microalgas Chlamydomonas para su posterior cultivo en una cámara acondicionada para el crecimiento de las microalgas bajo condiciones controladas de temperatura, fotoperiodo y radiación. Durante la etapa de cultivo en matraces de 500 mL se aplicaron cinco tratamientos con distinta concentración de nutrientes (solución nutritiva de Hoagland y Arnon II modificada, al 25, 50, 100, 150 y 200%). Se realizaron muestreos durante 31 días (día por medio), obteniendo curvas de crecimiento poblacional típicas de microorganismos, esto permitió discutir el efecto de la solución nutritiva en cuatro días representativos (días 9, 17, 25 y 31) del crecimiento poblacional. Durante estos cuatro días no se detectaron diferencias que pudieran indicar alguna tendencia o efecto de la concentración de la solución nutritiva sobre la producción de biomasa, ni sobre el contenido de proteínas solubles, hidratos de carbonos, clorofila a, clorofila b y carotenoides, durante el periodo de cultivo. La capacidad antioxidante tampoco se vio afectada por las distintas concentraciones de la solución nutritiva. Sobre la base de los resultados anteriores, se podría sugerir que el mejor momento para cosechar microalgas con fines comerciales correspondería el día 25, el cuál se sitúa hacia el final de la fase de crecimiento exponencial. Durante dicho día el tratamiento con la menor concentración de la solución nutritiva no presentó diferencias en biomasa fresca producida, contenido de proteínas solubles y carotenoides, como tampoco en capacidad antioxidante, en comparación con los otros tratamientos. De acuerdo a los resultados se puede concluir que las cinco concentraciones de la solución nutritiva permiten un adecuado crecimiento en Chlamydomonas, satisfaciendo sus requerimientos nutricionales para producción de biomasa fresca y de biomoléculas de interés comercial.; In this study was evaluated the effect of Hoagland and Arnon II nutrient solution in the production of fresh biomass, biomolecules content and the antioxidant capacity of the microalgae Chlamydomonas, genus that is commonly used as a research model system in cellular biology, however until now this studies didn’t analyses the… Advisors/Committee Members: Tapia Figueras, María Luisa (advisor), Riquelme E., Alejandro (advisor).

Subjects/Keywords: Biomasa; Microalgas; Proteínas; Antioxidantes; Chlamydomonas

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APA (6^th Edition):

Hun Arenas, Y. C. (2010). Efecto de la concentración de la solución nutritiva sobre la biomasa de microalgas del género Chlamydomonas . (Thesis). Universidad de Chile. Retrieved from http://www.repositorio.uchile.cl/handle/2250/111421

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hun Arenas, Yasmín Carolina. “Efecto de la concentración de la solución nutritiva sobre la biomasa de microalgas del género Chlamydomonas .” 2010. Thesis, Universidad de Chile. Accessed September 16, 2019. http://www.repositorio.uchile.cl/handle/2250/111421.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hun Arenas, Yasmín Carolina. “Efecto de la concentración de la solución nutritiva sobre la biomasa de microalgas del género Chlamydomonas .” 2010. Web. 16 Sep 2019.

Vancouver:

Hun Arenas YC. Efecto de la concentración de la solución nutritiva sobre la biomasa de microalgas del género Chlamydomonas . [Internet] [Thesis]. Universidad de Chile; 2010. [cited 2019 Sep 16]. Available from: http://www.repositorio.uchile.cl/handle/2250/111421.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hun Arenas YC. Efecto de la concentración de la solución nutritiva sobre la biomasa de microalgas del género Chlamydomonas . [Thesis]. Universidad de Chile; 2010. Available from: http://www.repositorio.uchile.cl/handle/2250/111421

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Tasmania

9. Vucica, Yvonne. Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum.

Degree: 2002, University of Tasmania

URL: https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf

Subjects/Keywords: Flagella (Microbiology); Chlamydomonas; Chlamydomonas reinhardtii

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APA (6^th Edition):

Vucica, Y. (2002). Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vucica, Yvonne. “Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum.” 2002. Thesis, University of Tasmania. Accessed September 16, 2019. https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vucica, Yvonne. “Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum.” 2002. Web. 16 Sep 2019.

Vancouver:

Vucica Y. Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum. [Internet] [Thesis]. University of Tasmania; 2002. [cited 2019 Sep 16]. Available from: https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vucica Y. Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum. [Thesis]. University of Tasmania; 2002. Available from: https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

10. Malnoë, Alizée. A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii.

Degree: Docteur es, Biologie, 2011, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2011PA112359

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Le cytochrome b6f est un complexe majeur de la chaîne photosynthétique oxygénique de par son activité quinol:plastocyanine oxydoréductase, qui contribue à la formation d’ATP via… (more)

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Le cytochrome b6f est un complexe majeur de la chaîne photosynthétique oxygénique de par son activité quinol:plastocyanine oxydoréductase, qui contribue à la formation d’ATP via un transfert d’électrons couplé à un transfert de protons. La présence d’un hème c particulier lié par une seule liaison covalente, l’hème ci, au sein du site de réduction de quinone Qi du cytochrome b6f constitue une différence notable en comparaison avec son homologue de la chaîne respiratoire, le cytochrome bc1. Un cytochrome b6f dépourvu d’hème ci est dégradé, sa faible accumulation ne permet pas une croissance photosynthétique. Cette observation a donné lieu à une recherche de suppresseurs permettant une plus grande accumulation de cytochrome b6f dont la fonction même altérée, serait suffisante pour assurer une croissance photosynthétique. Cette approche génétique de recherche de suppresseur a été entreprise chez Chlamydomonas reinhardtii. Ce travail de thèse a permis l’isolation et la caractérisation d’un mutant de la protéase FtsH1 (mutation R420C qui affecterait l’activité ATPasique). Le mutant ftsh1-1 s’est révélé être un outil puissant pour l’étude fonctionnelle de complexes mutés autrement dégradés. Une approche multidisciplinaire combinant expériences de génétique, biochimie, physiologie et biophysique a démontré notamment que : (i) le mutant QiKO, dont le complexe b6f est dépourvu des hèmes bh et ci, peut pousser de manière phototrophique malgré un Q-cycle cassé, (ii) l’absence d’hème ci lié covalemment, pour le mutant Rccb2, génère une photosensibilité exacerbée en présence d’oxygène, ce qui sous-tend un rôle pour l’hème ci dans un environnement riche en oxygène, (iii) la protéase FtsH exerce un contrôle qualité global des complexes majeurs photosynthétiques.

Central in oxygenic photosynthesis, the cytochrome b6f complex, couples electron transfer to proton translocation across the thylakoid membrane via its quinol:plastocyanin oxidoreductase activity, contributing to ATP formation. Cytochrome b6f complex differs from its respiratory homolog, the bc1 complex, by the presence of an additional heme, heme ci located within the quinone reduction site Qi and attached by a unique thioether bond. Mutants lacking heme ci show low accumulation of partially functional b6f complex and, hence, cannot grow phototrophically. This grounded a screen for suppressor mutations that would restore higher accumulation of b6f complexes whose function, even if compromised, would sustain phototrophic growth.The genetic suppressor approach undertook in Chlamydomonas reinhardtii during this PhD thesis led to the isolation and characterisation of the ftsh1-1 protease mutant (mutation R420C which should affect ATP hydrolysis). The mutant ftsh1-1 proved to be a versatile tool for the functional study of otherwise degraded proteins. The combination of genetic, biochemical, physiological and biophysical experiments demonstrated notably that: (i) a QiKO mutant, whose b6f complexes are devoid of both bh and ci hemes, can grow phototrophically despite a broken…

Advisors/Committee Members: Vitry, Catherine de (thesis director).

Subjects/Keywords: Photosynthèse; Cytochrome; Protéase; Chlamydomonas; Photosynthesis; Cytochrome; Protease; Chlamydomonas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Malnoë, A. (2011). A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2011PA112359

Chicago Manual of Style (16^th Edition):

Malnoë, Alizée. “A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii.” 2011. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed September 16, 2019. http://www.theses.fr/2011PA112359.

MLA Handbook (7^th Edition):

Malnoë, Alizée. “A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii.” 2011. Web. 16 Sep 2019.

Vancouver:

Malnoë A. A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2011. [cited 2019 Sep 16]. Available from: http://www.theses.fr/2011PA112359.

Council of Science Editors:

Malnoë A. A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2011. Available from: http://www.theses.fr/2011PA112359

11. Clowez, Sophie. Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii.

Degree: Docteur es, Biophysique, 2014, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2014PA066525

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Le processus photosynthétique se traduit par un flux d’électron impliquant différents complexes de la membrane thylacoïdale. Ce flux peut adopter deux chemins différents : le… (more)

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Le processus photosynthétique se traduit par un flux d’électron impliquant différents complexes de la membrane thylacoïdale. Ce flux peut adopter deux chemins différents : le transfert d’électron linéaire (Merchant, Prochnik et al. 2007) à travers lequel les électrons sont transférés de l’eau oxydée au niveau du Photosystème II (PSII), au NADPH réduit par le PSI ; et le transfert d’électron cyclique autour du Photosystème I (PSI) et du complexe cytochrome b6f. Ces flux d’électrons sont couplés à un pompage de proton du stroma vers le lumen générant une différence de potentiel transmembranaire, permettant la synthèse d’ATP (Allen 2002). La coexistence de ces deux flux est considéré comme nécessaire à la fixation et la métabolisation des molécules de dioxyde de carbone (Seelert, Poetsch et al. 2000 ; Munekage, Hashimoto et al. 2004) dans un rapport stricte ATP / NADPH. Cette coexistence qui semble essentiel soulève la question des mécanismes qui prévalent à l’implication des mêmes acteurs photosynthétiques, dans une même membrane, dans l’un ou l’autre mode de transfert d’électron. Chez l’algue verte Chlamydomonas reinhardtii, nous avons démontré que la commutation entre les deux transferts était dépendante de l’état redox des cellules, mais contrairement à ce qui avait été suggéré dans les études précédentes (Bulté, Rebeillé et al. 1990 ; Finazzi, Rappaport et al. 2002) indépendante du phénomène de transition d’état (Takahashi, Clowez et al. 2013), qui implique la migration latérale des complexes antennaires au sein de la membrane. L’association de ces antennes au Photosystème I conduirait à la séquestration, dans une même entité biochimique, des différents acteurs du mode cyclique. Cette formation de supercomplexe dans les conditions anoxiques, à fait l’objet d’une étude fonctionnelle in vitro, laissant quelques questions ouvertes sur leurs capacités fonctionnelles. Ce travail de thèse présente aussi la caractérisation d’une limitation transitoire des accepteurs du Photosystème I, en début d’anoxie pendant laquelle il n’est pas possible d’observer d’oxydation de P700, à 705 nm. Ce phénomène dû à la recombinaison de charge est créé par un engorgement du pool de NADPH. L’oxydation spontanée du PSI au bout d’un certain temps d’anoxie implique l’induction de l’hydrogénase, acceptant les électrons du PSI. Il reste possible d’induire cette évolution de l’oxydation de P700 lorsque les cellules sont constamment sous illumination dans les conditions anoxiques, impliquant cette fois ci, la voie de l’ATP chloroplastique. L’ATP synthétisé à la lumière permettrait la consommation de NADPH via le cycle de Benson Calvin.

The photosynthetic process relies on an electron flow involving several complexes in the thylakoid membranes of photosynthetic organisms. This flux can follow two possibly competing pathways: the linear electron transfer through which electrons are transferred from water (which is oxidized) to NADP+ (which is reduced), which is coupled to the generation of a transmembrane potential difference allowing the…

Advisors/Committee Members: Rappaport, Fabrice (thesis director).

Subjects/Keywords: Photosynthèse; Électron; Transfert; Spectrophotométrie; Métabolisme; Chlamydomonas; Photosynthesis; Electron; Chlamydomonas reinhardtii; 572.46

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clowez, S. (2014). Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2014PA066525

Chicago Manual of Style (16^th Edition):

Clowez, Sophie. “Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii.” 2014. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed September 16, 2019. http://www.theses.fr/2014PA066525.

MLA Handbook (7^th Edition):

Clowez, Sophie. “Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii.” 2014. Web. 16 Sep 2019.

Vancouver:

Clowez S. Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. [cited 2019 Sep 16]. Available from: http://www.theses.fr/2014PA066525.

Council of Science Editors:

Clowez S. Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. Available from: http://www.theses.fr/2014PA066525

The Ohio State University

12. Teplitski, Maxim Igorevich. Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing.

Degree: PhD, Graduate School, 2002, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062

Subjects/Keywords: Biology; Sinorhizobium; Chlamydomonas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teplitski, M. I. (2002). Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062

Chicago Manual of Style (16^th Edition):

Teplitski, Maxim Igorevich. “Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing.” 2002. Doctoral Dissertation, The Ohio State University. Accessed September 16, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062.

MLA Handbook (7^th Edition):

Teplitski, Maxim Igorevich. “Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing.” 2002. Web. 16 Sep 2019.

Vancouver:

Teplitski MI. Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing. [Internet] [Doctoral dissertation]. The Ohio State University; 2002. [cited 2019 Sep 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062.

Council of Science Editors:

Teplitski MI. Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing. [Doctoral Dissertation]. The Ohio State University; 2002. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062

Universidade do Rio Grande do Sul

13. Pizzoli, Guilherme. Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii.

Degree: 2016, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/170066

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Considerada a mais comum das doenças lisossômicas, a doença de Gaucher é causada por mutações no gene GBA1 que resultam na síntese defeituosa da enzima… (more)

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Considerada a mais comum das doenças lisossômicas, a doença de Gaucher é causada por mutações no gene GBA1 que resultam na síntese defeituosa da enzima glicocerebrosidase (GBA), responsável pela hidrólise dos glicocerebrosídios em glicose e ceramida. A deficiência da enzima provoca o acúmulo desses glicolipídios nos macrófagos, principalmente no fígado, no baço e na medula óssea, levando a um fenótipo complexo. O tratamento da doença consiste na administração da enzima GBA humana (HsGBA) exógena e, apesar de sua eficácia, é extremamente oneroso. Assim como outras espécies de microalgas, Chlamydomonas reinhardtii apresenta alto potencial para a produção de grandes quantidades de proteínas recombinantes de forma rápida e a um custo muito inferior ao dos sistemas de expressão tradicionais. O objetivo proposto para o desenvolvimento deste trabalho foi a expressão do gene codificador da HsGBA a partir do genoma nuclear de C. reinhardtii visando à produção da proteína como possível alternativa para a terapia de reposição enzimática. O gene HsGBA artificial foi projetado com códons adaptados para a expressão nuclear em C. reinhardtii e com sítios de restrição. A sequência nucleotídica foi sintetizada pela empresa GenScript USA Inc. e, após seu recebimento em plasmídeo de clonagem, o gene HsGBA foi inserido no plasmídeo pHsp70A/RbcS2-cgLuc por restrição com endonucleases e ligação. Esse vetor foi combinado com o plasmídeo pKS-aph7``-lox para a transferência do cassete de expressão do gene de interesse por recombinação sítio específica mediada pelo sistema Cre/lox, originando o vetor pKS-aph7``-lox::HsGBA, que contém, assim, os cassetes de expressão em tandem para HsGBA e para o marcador de seleção aph7``, codificador da enzima aminoglicosídeo fosfotransferase e capaz de conferir resistência à higromicina B. A linhagem de C. reinhardtii CC-400 cw15 mt+ foi transformada por eletroporação com o plasmídeo resultante linearizado (clivado com EcoRV) e na forma circular. A integração de HsGBA no genoma de cinco linhagens de C. reinhardtii foi comprovada por PCR e sua expressão foi demonstrada em três dessas linhagens de forma qualitativa por RT-PCR. Como HsGBA é controlado por um promotor induzível, hsp70A, diversas condições foram testadas visando à sua máxima expressão. Entretanto, análises por SDS-PAGE e western blot não permitiram a detecção da proteína recombinante. De modo semelhante, a atividade enzimática da HsGBA avaliada em extratos proteicos de linhagens transformadas não foi diferente da observada para linhagens não transformadas de C. reinhardtii.

Considered the most common lysosomal disorder, Gaucher disease is caused by mutations in GBA1 gene that result in defective synthesis of the enzyme glucocerebrosidase (GBA), responsible for the hydrolysis of glucocerebrosides into glucose and ceramide. When the enzyme is defective, these glycolipids accumulate in the macrophages, mainly in the liver, spleen and bone marrow, leading to a complex phenotype. Current treatment consists of enzyme replacement therapy by the…

Advisors/Committee Members: Pasquali, Giancarlo.

Subjects/Keywords: Glicocerebrosidase; Chlamydomonas reinhardtii; Doença de Gaucher

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pizzoli, G. (2016). Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/170066

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pizzoli, Guilherme. “Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii.” 2016. Thesis, Universidade do Rio Grande do Sul. Accessed September 16, 2019. http://hdl.handle.net/10183/170066.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pizzoli, Guilherme. “Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii.” 2016. Web. 16 Sep 2019.

Vancouver:

Pizzoli G. Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2016. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10183/170066.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pizzoli G. Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii. [Thesis]. Universidade do Rio Grande do Sul; 2016. Available from: http://hdl.handle.net/10183/170066

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Mera, Roi. El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff.

Degree: 2016, Universidade da Coruña

URL: http://hdl.handle.net/2183/16919

► [Abstract] Sulphate is the main source of sulphur for microalgae and, therefore, an essential nutrient for these organisms. The knowledge of the optimal concentration of… (more)

Subjects/Keywords: Chlamydomonas moewusii; Microalgas-Crecimiento; Cadmio-Toxicología

12 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mera, R. (2016). El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff. (Thesis). Universidade da Coruña. Retrieved from http://hdl.handle.net/2183/16919

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mera, Roi. “El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff.” 2016. Thesis, Universidade da Coruña. Accessed September 16, 2019. http://hdl.handle.net/2183/16919.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mera, Roi. “El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff.” 2016. Web. 16 Sep 2019.

Vancouver:

Mera R. El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff. [Internet] [Thesis]. Universidade da Coruña; 2016. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/2183/16919.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mera R. El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff. [Thesis]. Universidade da Coruña; 2016. Available from: http://hdl.handle.net/2183/16919

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wisconsin – Oshkosh

15. Ranatunga, Don Ruwan. EXPRESSION AND PURIFICATION OF THE Chlamydomonas BLUE-COPPER PLASTOCYANIN PROTEIN.

Degree: MS-Biology Microbiology, 2009, University of Wisconsin – Oshkosh

URL: http://digital.library.wisc.edu/1793/46732

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A Thesis Submitted In Partial Fulfillment of the Requirements For the Degree of Master of Science-Biology Microbiology

Plastocyanin (PC) is a type 1 blue copper… (more)

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A Thesis Submitted In Partial Fulfillment of the Requirements For the Degree of Master of Science-Biology Microbiology

Plastocyanin (PC) is a type 1 blue copper (Cu) protein found in the thylakoid lumen of plant and algal chloroplasts and in some cyanobacteria. It carries electrons from the cytochrome bf complex to the photosystem I reaction center. The ?-barrel structure of PC provides a binding site for the bound copper atom, which undergoes oxidation and reduction during electron transfer. In the oxidized state, the Cu center has a large absorbance peak centered at 597 nm, providing a convenient marker for purification and quantification of the protein. The atomic 3D structures of the Chlamydomonas reinhardtii cytochrome bf complex and PC have been solved. An abundant source of Chlamydomonas PC would be useful for detailed structure-function studies of electron transfer and interactions between these important electron transfer partners. I have expressed in Escherichia coli the Chlamydomonas PC as a His-tagged, thioredoxin fusion protein. The fusion protein, purified by metal-chelating and anion-exchange chromatography, carries a Cu atom as shown by its absorbance spectrum and has a characteristic redox midpoint potential of +375.5 + 1.5 mV at pH 7.0. However, the molar Cu content of the purified protein was only ~10%. One hypothesis for low Cu incorporation is that differences in cis-trans isomerization of proline 36 (in mature C. reinhardti PC) near the Cu pocket might hinder Cu binding. Pro36 is in the less common cis configuration in PC structures. This has been tested by site-directed mutations F35S and AGF33-35KLS that made the PC protein of the green alga Chlamydomonas more similar to that of the cyanobacterium Synechocystis sp. PCC 6803. Unfortunately, these modifications did not significantly improve Cu incorporation, nor did attempts at in vitro Cu reconstitution into the fusion protein nor the separated PC. Other possibilities for low Cu incorporation include interfering metal ion interactions during His-bind, Ni-chelating chromatography or steric constraints imposed by the thioredoxin fusion partner. Another approach for producing Chlamydomonas PC was the expression from plasmids that encode the pelB leader sequence for export of the native PC to the E. coli periplasm. Although the PC yield from these plasmids is low, this strategy might avoid problems that appear to be associated with the thioredoxin fusion protein and immobilized metal ion affinity chromatography.

Advisors/Committee Members: Haffa, Arlene, Kostman, Todd, Kallas, Toivo.

Subjects/Keywords: Electron transport; Copper proteins; Chlamydomonas; Plastocyanin

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APA (6^th Edition):

Ranatunga, D. R. (2009). EXPRESSION AND PURIFICATION OF THE Chlamydomonas BLUE-COPPER PLASTOCYANIN PROTEIN. (Masters Thesis). University of Wisconsin – Oshkosh. Retrieved from http://digital.library.wisc.edu/1793/46732

Chicago Manual of Style (16^th Edition):

Ranatunga, Don Ruwan. “EXPRESSION AND PURIFICATION OF THE Chlamydomonas BLUE-COPPER PLASTOCYANIN PROTEIN.” 2009. Masters Thesis, University of Wisconsin – Oshkosh. Accessed September 16, 2019. http://digital.library.wisc.edu/1793/46732.

MLA Handbook (7^th Edition):

Ranatunga, Don Ruwan. “EXPRESSION AND PURIFICATION OF THE Chlamydomonas BLUE-COPPER PLASTOCYANIN PROTEIN.” 2009. Web. 16 Sep 2019.

Vancouver:

Ranatunga DR. EXPRESSION AND PURIFICATION OF THE Chlamydomonas BLUE-COPPER PLASTOCYANIN PROTEIN. [Internet] [Masters thesis]. University of Wisconsin – Oshkosh; 2009. [cited 2019 Sep 16]. Available from: http://digital.library.wisc.edu/1793/46732.

Council of Science Editors:

Ranatunga DR. EXPRESSION AND PURIFICATION OF THE Chlamydomonas BLUE-COPPER PLASTOCYANIN PROTEIN. [Masters Thesis]. University of Wisconsin – Oshkosh; 2009. Available from: http://digital.library.wisc.edu/1793/46732

Ruhr Universität Bochum

16. Lambertz, Camilla Regina. Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii.

Degree: 2010, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037

► Das Ferredoxin-kodierende Gen FDX5 aus C. reinhardtii fällt durch einen besonders starken Expressionsanstieg unter Sauerstoff- und Kupfermangel auf. Über Reportergenanalysen konnten zwei GTAC-Motive für die… (more)

Subjects/Keywords: Chlamydomonas reinhardtii; Hydrogenase; Transkriptionsfaktor; Hypoxie; Anaerobiose

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lambertz, C. R. (2010). Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lambertz, Camilla Regina. “Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii.” 2010. Thesis, Ruhr Universität Bochum. Accessed September 16, 2019. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lambertz, Camilla Regina. “Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii.” 2010. Web. 16 Sep 2019.

Vancouver:

Lambertz CR. Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii. [Internet] [Thesis]. Ruhr Universität Bochum; 2010. [cited 2019 Sep 16]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lambertz CR. Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii. [Thesis]. Ruhr Universität Bochum; 2010. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Oh, Victor Hyun. EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/37085

► Green eukaryotic microalgae are an attractive economic, and efficient, alternative to conventional recombinant protein expression systems and have the potential to revolutionize the biotechnology industry.… (more)

▼ Green eukaryotic microalgae are an attractive economic, and efficient, alternative to conventional recombinant protein expression systems and have the potential to revolutionize the biotechnology industry. Recent advancements in the genomic and proteomic techniques of microalgae have ushered tremendous efforts to optimize the “upstream” side of process design through the use of promoters, codon-optimization, and plasmid design. While the prospective for microalgae as a molecular farming platform is high, there are still a number of major obstacles, namely inconsistent transgene expression, that must be overcome before it can be implemented as a standard expression system. The supplementation of culture medium with sodium butyrate to enhance transgene expression in mammalian cells for higher protein yields has been studied for several decades. Despite the wealth of literature available on the role of sodium butyrate in inducing changes of the morphology, growth rate, and gene expression in mammalian and plant cells, there is little information documented on its effect on green microalgal cells. In this study we explore the changes in cell morphology, cell growth, and transgene expression of a recombinant anti-apoptotic Venus-Bcl-xL protein in microalgae Chlamydomonas reinhardtii (C. reinhardtii) cells induced by sodium butyrate supplementation, and investigate the prospective of combining the advantages of a genetically modified anti-apoptotic C. reinhardtii strain with the transgene enhancing effects of sodium butyrate. We observed that concentrations of sodium butyrate above 250 mM were lethal for both the wild-type C. reinhardtii wall-deficient (CW 15+) UTEX 2337 strain and C. reinhardtii wall-deficient Venus-Bcl-xL (pRelax) strain. Concentrations of 50 mM and 100 mM induced phonotypical changes in the wild-type C. reinhardtii cell morphology induced apoptosis and inhibited cell growth. The difference in final cell density for cultures induced with 50 mM and 100 mM sodium butyrate from untreated cells averaged 5.79% and 62.92%, respectively. The anti-apoptotic pRelax C. reinhardtii strain showed significant signs of tolerance to apoptosis and cell growth inhibition at 50 mM and 100 mM sodium butyrate as cultures treated with 50 mM and 100 mM sodium butyrate were 75% and 78% less apoptotic than untreated pRelax cells, respectively, and difference in final cell density from untreated pRelax cells were -13.08% and 1.675%, respectively. The transgenic pRelax strain induced with 50 mM and 100 mM sodium butyrate showed substantial reduction in apoptotic behavior compared to untreated pRelax cells, indicating the possibility of up-regulation of the recombinant anti-apoptotic Venus-Bcl-xL protein caused by sodium butyrate induction. Advisors/Committee Members: Frachette, Joelle (advisor).

Subjects/Keywords: sodium butyrate; chlamydomonas; recombinant; bcl-xL

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APA (6^th Edition):

Oh, V. H. (2014). EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37085

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oh, Victor Hyun. “EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII.” 2014. Thesis, Johns Hopkins University. Accessed September 16, 2019. http://jhir.library.jhu.edu/handle/1774.2/37085.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oh, Victor Hyun. “EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII.” 2014. Web. 16 Sep 2019.

Vancouver:

Oh VH. EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2019 Sep 16]. Available from: http://jhir.library.jhu.edu/handle/1774.2/37085.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oh VH. EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37085

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Mitchell, Madeline Claire. Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii.

Degree: PhD, 2015, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/263885 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708522

► Despite accounting for approximately half of global primary productivity, photosynthesis in aquatic environments is often limited by the availability of dissolved carbon dioxide (CO_2(aq)). To… (more)

▼ Despite accounting for approximately half of global primary productivity, photosynthesis in aquatic environments is often limited by the availability of dissolved carbon dioxide (CO_2(aq)). To overcome the slow diffusion of CO_2(aq) as well as kinetic limitations of the primary photosynthetic carboxylase, Rubisco, carbon concentrating mechanisms (CCMs) have evolved in many aquatic photosynthetic organisms to improve photosynthetic efficiency and growth in CO2- limited environments. In the model eukaryotic green alga Chlamydomonas reinhardtii, the CCM is induced under low CO2 in the light and comprises: active inorganic carbon transport systems, carbonic anhydrases and the localisation of Rubisco to a central chloroplast microcompartment called the pyrenoid. In addition to changes in gene expression, acclimation to low CO2 is accompanied by alterations to metabolism, physiology and even cellular ultrastructure. However, mechanisms governing the regulation and interaction of these molecular components to increase CCM activity remain poorly understood. The overall aim of this study was to investigate regulation of the CCM in wild-type and mutant Chlamydomonas strains at both the whole cell and molecular level. Firstly, investigation of CCM induction in synchronised cultures of wild-type cells identified changes in CCM activity that were uncoupled from accumulation of CCM-related mRNA and protein, contrasting with the coordinated response to low CO2 observed in asynchronous control cultures. Pre-dawn induction of the CCM was coincident with preferential localisation of Rubisco and a thylakoid-lumenal carbonic anhydrase (CAH3) to the pyrenoid, highlighting the possible role of endogenous signals and post-translational modifications in modulating CCM activity. Secondly, in order to probe the relationship between pyrenoid formation and CCM induction and activity, CCM expression was investigated in pyrenoid-negative mutants with substituted Rubisco small subunits (RBCS). Low CO2-adapted pyrenoid-less RBCS mutants had impaired growth and low photosynthetic affinity for inorganic carbon (Ci). These pyrenoid-negative strains also showed a specific reduction in the accumulation of several CCM mRNAs, compared to pyrenoid- positive wild-type. Two-dimensional difference in gel electrophoresis (2D-DIGE) was used to compare the soluble proteome of one low CO2-adapted pyrenoid-less RBCS mutant compared to the pyrenoid-positive wild-type. This analysis identified only a few differentially expressed proteins, none of which were directly involved in CCM activity. Two primary metabolic enzymes were more abundant in the wild- type while eight proteins associated with protein synthesis and photosynthesis were more abundant in the pyrenoid-less mutant, suggesting that pyrenoid loss is accompanied by global metabolic, as well as CCM-specific, changes. A shotgun proteomics approach (LC-MS/MS) was used to extend the analysis of the pyrenoid-less RBCS mutant proteome to the whole genome level. Approximately 10% of the total proteins…

Subjects/Keywords: Chlamydomonas reinhardtii

…1.3 Chlamydomonas is the model eukaryotic green alga with a CCM ............ 5 1.3.1… …Chlamydomonas reinhardtii ........................................................................ 8… …OF FIGURES Figure 1.1 Transmission electron micrograph of wild-type Chlamydomonas… …of the CCM in Chlamydomonas reinhardtii.............................. 6 Figure 3.1… …Chlamydomonas reinhardtii ............................................... 130 xviii LIST OF…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mitchell, M. C. (2015). Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/263885 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708522

Chicago Manual of Style (16^th Edition):

Mitchell, Madeline Claire. “Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii.” 2015. Doctoral Dissertation, University of Cambridge. Accessed September 16, 2019. https://www.repository.cam.ac.uk/handle/1810/263885 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708522.

MLA Handbook (7^th Edition):

Mitchell, Madeline Claire. “Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii.” 2015. Web. 16 Sep 2019.

Vancouver:

Mitchell MC. Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. University of Cambridge; 2015. [cited 2019 Sep 16]. Available from: https://www.repository.cam.ac.uk/handle/1810/263885 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708522.

Council of Science Editors:

Mitchell MC. Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. [Doctoral Dissertation]. University of Cambridge; 2015. Available from: https://www.repository.cam.ac.uk/handle/1810/263885 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708522

Louisiana State University

19. Mukherjee, Bratati. Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii.

Degree: PhD, 2013, Louisiana State University

URL: etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722

► Photosynthetic microalgae optimize the utilization of inorganic carbon by active uptake and concentration of inorganic carbon (Ci) around the carbon-fixing enzyme Rubisco. This process, mostly… (more)

▼ Photosynthetic microalgae optimize the utilization of inorganic carbon by active uptake and concentration of inorganic carbon (Ci) around the carbon-fixing enzyme Rubisco. This process, mostly induced under limiting carbon conditions is called the Carbon Concentrating Mechanism or CCM. The photosynthetic green alga Chlamydomonas reinhardtii serves as an excellent model organism for the study of eukaryotic CCMs. However, unlike the prokaryotic cyanobacterial CCM, which has a well-characterized Ci uptake system, the Ci uptake and transport system of the C. reinhardtii CCM is not well understood. Since CO2 is a small neutral molecule, it is believed to be able to passively diffuse into the cell with or without any assistance from membrane bound transport proteins. However, the charged HCO3- ion needs transporters to facilitate its uptake across each membrane barrier. The hydrophobic barriers posed by the plasma membrane, chloroplast envelope and thylakoid membranes in the path of the charged HCO3- ion’s ultimate destination to the thylakoid lumen has led to the proposed existence of one or more transport proteins at each of these membrane locations. The roles of the C. reinhardtii NAR gene family, showing sequence homology to the Formate/Nitrite transporter family, were investigated for any changes with respect to the induction of the CCM. NAR1.2 was found to be the most interesting NAR protein with respect to the CCM. NAR1.2 was localized to the chloroplast envelope and is believed to be part of the chloroplastic Ci uptake system. The previously identified putative Ci transporter, LCI1 was localized to the plasma membrane. The expression of this protein in a LCI1 deficient background showed a significant increase in Ci uptake and Ci affinity of cells even in the absence of a functional CCM. However, the RNAi mediated knockdown of the protein failed to show any growth deficiencies or changes in photosynthetic rates at different pH levels. LCI1 is a transporter that is part of the plasma membrane Ci uptake system. In the quest for mutations in either previously identified or novel Ci transporters, an insertional mutagenesis project was also undertaken. The sequenced wild-type strain, CC-503, was found to be a natural mutant for the periplasmic carbonic anhydrase, CAH1.

Subjects/Keywords: insertional mutagenesis; Chlamydomonas reinhardtii; inorganic carbon; CCM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mukherjee, B. (2013). Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722

Chicago Manual of Style (16^th Edition):

Mukherjee, Bratati. “Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii.” 2013. Doctoral Dissertation, Louisiana State University. Accessed September 16, 2019. etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722.

MLA Handbook (7^th Edition):

Mukherjee, Bratati. “Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii.” 2013. Web. 16 Sep 2019.

Vancouver:

Mukherjee B. Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Louisiana State University; 2013. [cited 2019 Sep 16]. Available from: etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722.

Council of Science Editors:

Mukherjee B. Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Louisiana State University; 2013. Available from: etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722

Louisiana State University

20. Tural, Baran. Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii.

Degree: MS, 2005, Louisiana State University

URL: etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179

► The regulation of the photorespiratory pathway of Chlamydomonas reinhardtii during a shift from high to low CO2 conditions was investigated. To this end, a set… (more)

Subjects/Keywords: Chlamydomonas reinhardtii; photorespiration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tural, B. (2005). Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii. (Masters Thesis). Louisiana State University. Retrieved from etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179

Chicago Manual of Style (16^th Edition):

Tural, Baran. “Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii.” 2005. Masters Thesis, Louisiana State University. Accessed September 16, 2019. etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179.

MLA Handbook (7^th Edition):

Tural, Baran. “Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii.” 2005. Web. 16 Sep 2019.

Vancouver:

Tural B. Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii. [Internet] [Masters thesis]. Louisiana State University; 2005. [cited 2019 Sep 16]. Available from: etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179.

Council of Science Editors:

Tural B. Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii. [Masters Thesis]. Louisiana State University; 2005. Available from: etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179

University of Illinois – Chicago

21. Elgass, Laura Jane. Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments.

Degree: 2014, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/19156

► Microfluidic platforms are excellent tools for studying the effects of precisely controlled oxygen and other gas environments on various biological systems, from mammalian cell culture… (more)

Subjects/Keywords: microfluidics; chlamydomonas reinhardtii; volvox carteri; oxygen control

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APA (6^th Edition):

Elgass, L. J. (2014). Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/19156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elgass, Laura Jane. “Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments.” 2014. Thesis, University of Illinois – Chicago. Accessed September 16, 2019. http://hdl.handle.net/10027/19156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elgass, Laura Jane. “Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments.” 2014. Web. 16 Sep 2019.

Vancouver:

Elgass LJ. Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments. [Internet] [Thesis]. University of Illinois – Chicago; 2014. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10027/19156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elgass LJ. Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments. [Thesis]. University of Illinois – Chicago; 2014. Available from: http://hdl.handle.net/10027/19156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Wilson, Kate. Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure.

Degree: PhD, Mechanical Engineering & Materials Science, 2015, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/eng_etds/102

► Cilia and flagella are subcellular organelles used to generate fluid flow or propel the cell. These long cylindrical structures are composed of cytoskeletal elements… (more)

Subjects/Keywords: Axoneme; Chlamydomonas; Cilia; Flagella; Modeling; Viscosity; Engineering

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APA (6^th Edition):

Wilson, K. (2015). Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/102

Chicago Manual of Style (16^th Edition):

Wilson, Kate. “Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure.” 2015. Doctoral Dissertation, Washington University in St. Louis. Accessed September 16, 2019. https://openscholarship.wustl.edu/eng_etds/102.

MLA Handbook (7^th Edition):

Wilson, Kate. “Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure.” 2015. Web. 16 Sep 2019.

Vancouver:

Wilson K. Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2015. [cited 2019 Sep 16]. Available from: https://openscholarship.wustl.edu/eng_etds/102.

Council of Science Editors:

Wilson K. Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure. [Doctoral Dissertation]. Washington University in St. Louis; 2015. Available from: https://openscholarship.wustl.edu/eng_etds/102

Cornell University

23. Zimmer, Sara. Organellar ribonucleases and poly(A) polymerases in Chlamydomonas reinhardtii .

Degree: 2008, Cornell University

URL: http://hdl.handle.net/1813/11073

► RNA catabolism occurs in multiple cellular compartments. This study used the unicellular photosynthetic eukaryote Chlamydomonas reinhardtii to identify a small suite of putatively organellar, nucleus-encoded… (more)

▼ RNA catabolism occurs in multiple cellular compartments. This study used the unicellular photosynthetic eukaryote Chlamydomonas reinhardtii to identify a small suite of putatively organellar, nucleus-encoded prokaryotic-like ribonucleases and poly(A) polymerases (PAPs) that may participate in this process. Other related genes encoded putative exosome components, but no nucleus or cytosol-specific exosome activating factors. A surprisingly large family of nine non-canonical PAPs were hypothesized to play a regulatory role in RNA degradation. The well-studied E. coli poly(A)-mediated RNA degradation pathway was the model used to predict and test the roles of candidate genes in chloroplast RNA catabolism. The pathway initiates with an endonucleolyic cleavage event, followed by polyadenylation of the resultant 3' end, which stimulates 5' to 3' exoribonucleolytic degradation. Unlike E. coli, Chlamydomonas has no RNase E and thus utilizes another enzyme, possibly RNase J1, as an endonuclease. Transient depletion of RNJ1 mRNA showed a very mild effect on chloroplast transcripts. As in E. coli, one hydrolytic (RNB2) and one phosphorolytic (Polynucleotide phosphorylase: PNPase) exoribonuclease may play a role in RNA decay. PNPase depletion caused an increase in RNB2 expression, but no profound alterations in the processing or steady state level of chloroplast RNA that would be expected if it were the sole exoribonuclease. Thus, it was concluded that there is likely to be some functional redundancy between these enzymes. PNPase depletion also revealed unique functional aspects, since it resulted in defects in acclimation to phosphate limitation, and was shown to be responsible for both polyadenylation and poly(A) tail degradation in the chloroplast. Comparative analysis of Arabidopsis and Chlamydomonas nucleotidyltransferases suggested that Chlamydomonas PAP4 but not PAP3 is likely to be mitochondrially localized. Recombinant PAP4 has polyadenylation activity, and when expressed in E. coli can partially complement a PAP1 (pcnB) mutant. Although PAP4 appeared to be mitochondrially targeted, its depletion affected neither mitochondrial poly(A) nor novel U-rich tails. Taken together, these studies have identified candidate genes encoding ribonucleases and poly(A) polymerases, elucidated some of the functions of PNPase in the chloroplast, characterized the biochemical activity of PAP4, and characterized polynucleotide tails in the mitochondria of Chlamydomonas reinhardtii.

Subjects/Keywords: Chlamydomonas; chloroplast; mitochondria; RNA degradation; ribonuclease; polyadenylation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zimmer, S. (2008). Organellar ribonucleases and poly(A) polymerases in Chlamydomonas reinhardtii . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/11073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zimmer, Sara. “Organellar ribonucleases and poly(A) polymerases in Chlamydomonas reinhardtii .” 2008. Thesis, Cornell University. Accessed September 16, 2019. http://hdl.handle.net/1813/11073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zimmer, Sara. “Organellar ribonucleases and poly(A) polymerases in Chlamydomonas reinhardtii .” 2008. Web. 16 Sep 2019.

Vancouver:

Zimmer S. Organellar ribonucleases and poly(A) polymerases in Chlamydomonas reinhardtii . [Internet] [Thesis]. Cornell University; 2008. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1813/11073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zimmer S. Organellar ribonucleases and poly(A) polymerases in Chlamydomonas reinhardtii . [Thesis]. Cornell University; 2008. Available from: http://hdl.handle.net/1813/11073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Lachapelle, Josianne Lyse. Role of chance and history during evolution in Chlamydomonas reinhardtii.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/15949

► The extent to which evolution is repeatable has important implications. If evolution is highly repeatable, the trajectories and outcomes of evolution in different lineages will… (more)

▼ The extent to which evolution is repeatable has important implications. If evolution is highly repeatable, the trajectories and outcomes of evolution in different lineages will always be the same. On the other hand, if evolution is not repeatable, then trajectories and outcomes will be diverse. Thus, the repeatability of evolution affects our understanding of the nature of biodiversity and can inform the extent to which evolutionary theory can be used to make predictions. The repeatability of evolution depends on the relative contribution of selection, chance, and history. To determine what factors affect the importance of chance and history during evolution, I propagated replicated populations of the unicellular green alga Chlamydomonas reinhardtii in controlled environments. I measured the change in fitness after a few hundred generations and determined how much variation had arisen among replicate populations and among populations with different histories. I applied a similar approach to study the importance of history in extinctions, and measured rates of extinction in populations with different histories. I found that evolution is much less repeatable in small than in large populations because history is more constraining and selection less efficient in small than in large populations. There is also a significant effect of sex and recombination on the repeatability of evolution at the fitness level, but this effect is highly dependent on the environment of selection. Sex can increase the importance of chance or history in some environments, but lower their importance in others, thereby leading to convergence or divergence depending on the environment. Thirdly, I found that the importance of history during evolution does not appear to come from the accumulation of past evolutionary selection pressures, but rather comes from only the most recent selection pressure as it determines genetic correlations for growth between different environments and the amount of genetic variance. Finally, I found that extinction risks are extremely high during continuous environmental deterioration, although a history of sexual reproduction and phenotypic plasticity play an important role in adaptation. By focusing not solely on the effect of treatments on mean trait values, but also on the variance that arises in our evolution experiments, we can gain a better understanding of the contribution that chance and history make to evolution. The repeatability of evolution can therefore inform us about the adaptive vs. stochastic nature of the diversity we see today, and about the specificity or generality of evolutionary outcomes.

Subjects/Keywords: 579.5; Chlamydomonas reinhardtii; evolution; deterministic; adaption

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APA (6^th Edition):

Lachapelle, J. L. (2016). Role of chance and history during evolution in Chlamydomonas reinhardtii. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/15949

Chicago Manual of Style (16^th Edition):

Lachapelle, Josianne Lyse. “Role of chance and history during evolution in Chlamydomonas reinhardtii.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed September 16, 2019. http://hdl.handle.net/1842/15949.

MLA Handbook (7^th Edition):

Lachapelle, Josianne Lyse. “Role of chance and history during evolution in Chlamydomonas reinhardtii.” 2016. Web. 16 Sep 2019.

Vancouver:

Lachapelle JL. Role of chance and history during evolution in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1842/15949.

Council of Science Editors:

Lachapelle JL. Role of chance and history during evolution in Chlamydomonas reinhardtii. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/15949

University of California – Berkeley

25. McSpadden, Ethan DeNardo. Structural and evolutionary studies on CaMKII oligomerization.

Degree: Molecular & Cell Biology, 2018, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/00k0r2pt

► Calcium-calmodulin-dependent protein kinase II (CaMKII) plays a critical role in animal learning and memory (Giese et al. 1998). CaMKII operates as a multi-subunit holoenzyme and… (more)

▼ Calcium-calmodulin-dependent protein kinase II (CaMKII) plays a critical role in animal learning and memory (Giese et al. 1998). CaMKII operates as a multi-subunit holoenzyme and can detect the frequency of incident Ca2+ pulse trains entering the cell (Chao et al. 2011; De Koninck and Schulman 1998). CaMKII holoenzymes become destabilized and appear to exchange subunits following catalytic activation, which results in the spread of kinase activity to inactive holoenzymes (Stratton et al. 2014; Bhattacharyya et al. 2016). This phenomenon is not fully understood and is under active investigation.This work focuses on the structural and biochemical characterization of CaMKII hub domain assemblies, which are essential to holoenzyme formation (Shen and Meyer 1998; Kolb et al. 1998). The crystal structure of a dodecameric human CaMKII-α hub domain assembly shows how the protein may be able to sense the activation state of the CaMKII kinase domain. This helps explain why kinase activation destabilizes CaMKII holoenzymes and promotes subunit exchange.The characterization of CaMKII hub domains from evolutionarily divergent organisms is also presented. Hub domains encoded by three related green algae assemble into 16-, 18-, and 20-subunit oligomers. These are the largest known CaMKII hub domain assemblies. A crystal structure of one, the Chlamydomonas reinhardtii 18-mer, revealed multiple intra-chain hydrogen bonds not present in the human isoform. When these hydrogen bonds were engineered into the human CaMKII hub domain by mutation the protein oligomerized into larger assemblies. A larger CaMKII holoenzyme is predicted to be more resistant to deactivation by protein phosphatases and thus may be useful in studying the consequences of aberrant CaMKII activity. These hydrogen bonds are also predicted to inhibit subunit exchange in human CaMKII by dampening structural fluctuations in the hub domain.Work is also described on an interfacial mutation in the hub domain that weakens the integrity of the CaMKII holoenzyme. CaMKII dimers are present in solution as a result of this mutation. Catalytic activation increases the dimer population, demonstrating that the hub domain interface weakened by the mutation is further destabilized when CaMKII is active. This suggests that dimer release is a central component of activation-triggered subunit exchange between CaMKII holoenzymes.

Subjects/Keywords: Biochemistry; CaMKII; Chlamydomonas; Human; Protein; Structure

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APA (6^th Edition):

McSpadden, E. D. (2018). Structural and evolutionary studies on CaMKII oligomerization. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/00k0r2pt

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McSpadden, Ethan DeNardo. “Structural and evolutionary studies on CaMKII oligomerization.” 2018. Thesis, University of California – Berkeley. Accessed September 16, 2019. http://www.escholarship.org/uc/item/00k0r2pt.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McSpadden, Ethan DeNardo. “Structural and evolutionary studies on CaMKII oligomerization.” 2018. Web. 16 Sep 2019.

Vancouver:

McSpadden ED. Structural and evolutionary studies on CaMKII oligomerization. [Internet] [Thesis]. University of California – Berkeley; 2018. [cited 2019 Sep 16]. Available from: http://www.escholarship.org/uc/item/00k0r2pt.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McSpadden ED. Structural and evolutionary studies on CaMKII oligomerization. [Thesis]. University of California – Berkeley; 2018. Available from: http://www.escholarship.org/uc/item/00k0r2pt

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

26. Belzile, Olivier. The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization.

Degree: 2012, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/984

► The cellular and molecular mechanisms specifying the membrane protein composition of cilia and flagella at steady state and during cilium-generated signaling are poorly understood. Our… (more)

▼ The cellular and molecular mechanisms specifying the membrane protein composition of cilia and flagella at steady state and during cilium-generated signaling are poorly understood. Our laboratory uses the biflagellated green alga Chlamydomonas as a model system to study regulated movement of the flagellar adhesion receptor SAG1 from the cell body to the flagella. Interactions between the plus flagellar receptor (agglutinin) SAG1 and its cognate receptor SAD1 on flagella of minus gametes induces flagellar adhesion and activation of a cAMP-dependent signaling pathway ultimately leading to cell-cell fusion. Although previous work from our laboratory and others suggested that pathway activation triggers mobilization of a pool of SAG1 from the cell body to the flagella, those studies depended on indirect adhesion bioassays detecting the activity of SAG1, not the protein. Here, I report use of new tools to study directly the regulation of SAG1 localization. I show that the SAG1 gene bearing a C-terminal HA tag rescues flagellar adhesion and cell fusion in the flagellar adhesion mutant, sag1-5. Immunofluorescence studies of resting SAG1-HA/sag1-5 mt+ plus gametes show that the protein is present mostly on cell bodies. Biochemical studies show that only gametes express SAG1-HA. Detection of precursor forms indicates SAG1 undergoes cleavage soon after its synthesis to yield an HA-tagged 65 kDa, C-terminal portion (SAG1-HA-C65), and that SAG1-HA-C65 is on the cell surface. Consistent with the predicted 3 transmembrane domains at the C-terminus, release of SAG1-HA-C65 in a soluble form requires detergent and is not achieved upon mechanical disruption, high salt, or high pH treatments. Cell fractionation demonstrates that in resting gametes the majority of SAG1-HA-C65 is present on cell bodies and only a small amount is on flagella. Within minutes after signaling is triggered by flagellar adhesion, however, SAG1-HA-C65 is mobilized from the cell body to the flagella and the organelles become highly enriched in the protein, where it forms large detergent-resistant complexes. I show that Chlamydomonas can regulate the amount of SAG1 in the flagella through cilium-generated signaling, and thus provides the first system for studying regulation of the membrane protein composition of the cilium/flagellum in a biochemically tractable system. Advisors/Committee Members: Snell, William J..

Subjects/Keywords: Cell Surface Extensions; Protozoan Proteins; Chlamydomonas

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APA (6^th Edition):

Belzile, O. (2012). The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/984

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Belzile, Olivier. “The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed September 16, 2019. http://hdl.handle.net/2152.5/984.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Belzile, Olivier. “The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization.” 2012. Web. 16 Sep 2019.

Vancouver:

Belzile O. The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/2152.5/984.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belzile O. The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/984

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

27. VanderWaal Mills, Kristyn E. Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii.

Degree: PhD, Molecular, Cellular, Developmental Biology and Genetics, 2011, University of Minnesota

URL: http://hdl.handle.net/11299/165777

► Cilia and flagella are microtubule-based organelles that perform critical functions in human health and development. The I1 inner arm dynein and the IC138 subunit play… (more)

▼ Cilia and flagella are microtubule-based organelles that perform critical functions in human health and development. The I1 inner arm dynein and the IC138 subunit play a key role in the regulation of flagellar motility. To understand how the IC138 protein and its associated subunits modulate I1 activity, we characterized the molecular lesions and motility phenotypes of several bop5 alleles in Chlamydomonas reinhardtii. We first characterize a mutation (bop5-2) that disrupts an IC138 protein sub-complex located at the base of the I1 inner arm dynein. We found the bop5-2 deletion also affects the Tubby-1 (TBY1) gene. To characterize TBY1's activity, tagged versions of TBY1 were transformed into bop5-2. TBY1 protein localizes to a unique ring shaped structure found between the two contractile vacuoles and within the nucleo-flagellar apparatus. The bop5-3, bop5-4 and bop5-5 strains, like other I1 mutants, swim forwards with reduced swimming velocities and display an impaired reversal response during photoshock. However, unlike mutants lacking the entire I1 complex, bop5 strains exhibit normal phototaxis. Analysis of the bop5-3 flagellar waveform reveals that loss of the IC138 sub-complex reduces shear amplitude, sliding velocities, and the speed of bend propagation. The results indicate that the IC138 sub-complex is necessary to generate an efficient waveform for optimal forwards and backwards motility, but it is not essential for phototaxis. Assembly and maintenance of eukaryotic cilia and flagella requires the conserved, bidirectional movement of protein complexes along the length of the axoneme known as intraflagellar transport (IFT). We characterize the function of various components of the IFT complex responsible for the retrograde transport of particles towards the cell body. We quantify the defects in retrograde IFT and flagella assembly observed in a series of mutants of the retrograde complex subunit LIC. We also analyze the expression and distribution of retrograde IFT components in a family of flagellar assembly mutants known as fla, and attempt to correlate these patterns with defects in IFT parameters and other behavioral phenotypes. We provide new evidence that defects in IFT motors can alter photoshock and phototaxis behaviors.

Subjects/Keywords: Chlamydomonas; Dynein; Flagella; Intraflagellar transport; Motility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

VanderWaal Mills, K. E. (2011). Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/165777

Chicago Manual of Style (16^th Edition):

VanderWaal Mills, Kristyn E. “Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii.” 2011. Doctoral Dissertation, University of Minnesota. Accessed September 16, 2019. http://hdl.handle.net/11299/165777.

MLA Handbook (7^th Edition):

VanderWaal Mills, Kristyn E. “Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii.” 2011. Web. 16 Sep 2019.

Vancouver:

VanderWaal Mills KE. Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. University of Minnesota; 2011. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/11299/165777.

Council of Science Editors:

VanderWaal Mills KE. Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii. [Doctoral Dissertation]. University of Minnesota; 2011. Available from: http://hdl.handle.net/11299/165777

University of Cambridge

28. Meyer, Moritz. Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid .

Degree: 2010, University of Cambridge

URL: http://www.dspace.cam.ac.uk/handle/1810/226160

► Aquatic photosynthesis accounts for 50% of the global annual net primary production (NPP), despite frequent low availability and limited diffusion of CO2 in the aquatic… (more)

▼ Aquatic photosynthesis accounts for 50% of the global annual net primary production (NPP), despite frequent low availability and limited diffusion of CO2 in the aquatic milieu, and low affinity for CO2 by the primary carboxylating enzyme, Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Many eukaryotic algae, and a single group of land plants, the hornworts, have an inducible carbon concentrating mechanism (CCM), to overcome these limitations. The efficiency of the CCM is improved when RuBisCO is localised to a subcellular compartment, the pyrenoid, which is hypothesised to act as a diffusion barrier for CO2 . Although the pyrenoid is a major player in global carbon balance (we estimate 10-15% of NPP), it is one of the few remaining prominent cellular features without a precise molecular or physiological definition. Under ambient CO2 , at least 90% of the cellular RuBisCO is packed into a dense matrix, together with the chaperone RuBisCO activase. Thylakoid membranes usually traverse the pyrenoid matrix, and the carboxylating substrate is thought to be delivered to the active sites of the enzyme via a carbonic anhydrase located in the lumen of these thylakoids. The mechanism of aggregation of constituents within the pyrenoid, however, still remains largely unknown. Comprehensive mutant screens have yet to reveal mutants incapable of forming pyrenoids other than those mutants with a defective RuBisCO holoenzyme, whereas DNA microarray studies uncovered little with reference to pyrenoid ultrastructure or aggregation. Taken together, this evidence raises the possibility that the basis of pyrenoid ultrastructure and aggregation lies entirely in sequence variations of RuBisCO itself. This work explored, firstly, the advantages conferred by an active CCM in hornworts and in unicellular algae, compared with the passive CO2 acquisition in most terrestrial plants. A physiological framework to CCM and pyrenoid-based photosynthesis, and isotopic discrimination, was provided by comparing the photosynthetic characteristics of selected bryophytes and algae, differing in chloroplast morphology and degrees of internalisation of gas exchanges. The results showed that on-line, carbon isotope discrimination values were a good indicator of CCM occurrence, as well as liquid-phase diffusion limitation, and biochemical limitations resulting from declining RuBisCO activity and electron transport. The methodology was used to diagnose the presence of an active CCM, and the extent of CO2 leakage. Secondly, the effect of RuBisCO sequence variations on the pyrenoid, and associated CCM, was studied using the model alga Chlamydomonas reinhardtii. The starting premise was the report by Nozaki et al. (2002) that, in some species of the family Chlamydomonaceae, a few amino acid residues within the RuBisCO large subunit (LSU) correlated strongly with pyrenoid formation. The specific roles of seven LSU residues were studied by site-directed mutagenesis. Whilst the mutations reduced the affinity of RuBisCO for CO2 and increased CO2 leakage,… Advisors/Committee Members: Griffiths, Howard (advisor).

Subjects/Keywords: Pyrenoid; Rubisco; Carbon concentrating mechanism; Chlamydomonas; RbcS

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APA (6^th Edition):

Meyer, M. (2010). Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid . (Thesis). University of Cambridge. Retrieved from http://www.dspace.cam.ac.uk/handle/1810/226160

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Meyer, Moritz. “Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid .” 2010. Thesis, University of Cambridge. Accessed September 16, 2019. http://www.dspace.cam.ac.uk/handle/1810/226160.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Meyer, Moritz. “Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid .” 2010. Web. 16 Sep 2019.

Vancouver:

Meyer M. Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid . [Internet] [Thesis]. University of Cambridge; 2010. [cited 2019 Sep 16]. Available from: http://www.dspace.cam.ac.uk/handle/1810/226160.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meyer M. Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid . [Thesis]. University of Cambridge; 2010. Available from: http://www.dspace.cam.ac.uk/handle/1810/226160

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

29. Thompson, Mark David. Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii .

Degree: 2016, University of Arizona

URL: http://hdl.handle.net/10150/620817

► The eyespot of the single-celled alga Chlamydomonas aids the cell in detecting the direction of light in the environment. The complex assembly and asymmetric placement… (more)

Subjects/Keywords: ChR1; Eyespot; Molecular & Cellular Biology; Chlamydomonas

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APA (6^th Edition):

Thompson, M. D. (2016). Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/620817

Chicago Manual of Style (16^th Edition):

Thompson, Mark David. “Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii .” 2016. Doctoral Dissertation, University of Arizona. Accessed September 16, 2019. http://hdl.handle.net/10150/620817.

MLA Handbook (7^th Edition):

Thompson, Mark David. “Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii .” 2016. Web. 16 Sep 2019.

Vancouver:

Thompson MD. Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii . [Internet] [Doctoral dissertation]. University of Arizona; 2016. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10150/620817.

Council of Science Editors:

Thompson MD. Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii . [Doctoral Dissertation]. University of Arizona; 2016. Available from: http://hdl.handle.net/10150/620817

University of Arizona

30. Boyd, Joseph Samuel. Eyespot Assembly and Positioning in Chlamydomonas reinhardtii .

Degree: 2011, University of Arizona

URL: http://hdl.handle.net/10150/145298

► The eyespot of the biflagellate unicellular green alga Chlamydomonas reinhardtii is a complex organelle that facilitates directional responses of the cell to environmental light stimuli.… (more)

▼ The eyespot of the biflagellate unicellular green alga Chlamydomonas reinhardtii is a complex organelle that facilitates directional responses of the cell to environmental light stimuli. The eyespot, which assembles de novo after every cell division and retains a distinctive association with the microtubule cytoskeleton, comprises an elliptical patch of rhodopsin photoreceptors in the plasma membrane and stacks of carotenoid-rich pigment granule arrays in the chloroplast and serves as a model for understanding how organelles are formed and placed asymmetrically in the cell. This study describes the roles of several factors in the assembly and positioning of the eyespot. Two loci, EYE2 and EYE3, define factors involved in the formation and organization of the eyespot pigment granule arrays. Whereas EYE3, a serine/threonine kinase of the ABC1 family, localizes to pigment granules, EYE2 localization corresponds to an area of the chloroplast envelope in the eyespot. These proteins play interdependent roles: EYE2 and the ChR1 photoreceptor co-position in the absence of pigment granules, and the pigment granules are required to maintain the shape and integrity of the EYE2/ChR1 patch. The miniature-eyespot locus MIN2 affects eyespot size and likely regulates the amount of material available for eyespot assembly. The MLT2 locus regulates eyespot size, number, and asymmetry. A novel locus, PEY1, modulates the position of the eyespot on the anterior-posterior axis by affecting microtubule rootlet length. A working model is developed wherein rootlet microtubule-directed photoreceptor localization establishes connections in the chloroplast envelope with EYE2, which directs the site for pigment granule array assembly, and MLT2 is proposed to negatively regulate the levels of eyespot proteins. Advisors/Committee Members: Dieckmann, Carol L (advisor), Weinert, Ted (committeemember), Tax, Frans E. (committeemember), Nagy, Lisa (committeemember), Fares, Hanna (committeemember).

Subjects/Keywords: Chlamydomonas; eyespot; microtubule rootlet; organelle biogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boyd, J. S. (2011). Eyespot Assembly and Positioning in Chlamydomonas reinhardtii . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/145298

Chicago Manual of Style (16^th Edition):

Boyd, Joseph Samuel. “Eyespot Assembly and Positioning in Chlamydomonas reinhardtii .” 2011. Doctoral Dissertation, University of Arizona. Accessed September 16, 2019. http://hdl.handle.net/10150/145298.

MLA Handbook (7^th Edition):

Boyd, Joseph Samuel. “Eyespot Assembly and Positioning in Chlamydomonas reinhardtii .” 2011. Web. 16 Sep 2019.

Vancouver:

Boyd JS. Eyespot Assembly and Positioning in Chlamydomonas reinhardtii . [Internet] [Doctoral dissertation]. University of Arizona; 2011. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10150/145298.

Council of Science Editors:

Boyd JS. Eyespot Assembly and Positioning in Chlamydomonas reinhardtii . [Doctoral Dissertation]. University of Arizona; 2011. Available from: http://hdl.handle.net/10150/145298

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Open Access Theses and Dissertations