subject:(Chlamydomonas)

University of British Columbia

1. Butler, Gary Lee. Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae).

Degree: MS- MSc, Botany, 1970, University of British Columbia

► An investigation of an obligate psychrophilic green alga, Chlamydomonas sp., was undertaken to define some of its growth characteristics. It has a temperature optimum of… (more)

Subjects/Keywords: Chlamydomonas

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APA (6^th Edition):

Butler, G. L. (1970). Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae). (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/34567

Chicago Manual of Style (16^th Edition):

Butler, Gary Lee. “Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae).” 1970. Masters Thesis, University of British Columbia. Accessed March 03, 2021. http://hdl.handle.net/2429/34567.

MLA Handbook (7^th Edition):

Butler, Gary Lee. “Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae).” 1970. Web. 03 Mar 2021.

Vancouver:

Butler GL. Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae). [Internet] [Masters thesis]. University of British Columbia; 1970. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2429/34567.

Council of Science Editors:

Butler GL. Preliminary studies on the physiology of a psychrophilic Chlamydomonas (Chlorophyceae). [Masters Thesis]. University of British Columbia; 1970. Available from: http://hdl.handle.net/2429/34567

University of Texas Southwestern Medical Center

2. Hernandez Lara, Carmen Isabel. Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1217

► The primary cilium is a signaling organelle found on most cell types in vertebrates. It is a distinct compartment for membrane and cytoplasmic proteins that… (more)

▼ The primary cilium is a signaling organelle found on most cell types in vertebrates. It is a distinct compartment for membrane and cytoplasmic proteins that mediate multiple sensory processes. Little is known about the cellular and molecular mechanisms that determine regulated ciliary localization of membrane or soluble proteins. Our laboratory uses the biflagellated green alga Chlamydomonas as a model system to study flagellar adhesion-induced signaling. During fertilization, Chlamydomonas mating type plus and mating type minus gametes undergo flagellar adhesion through the interaction of flagellar adhesion receptor activities (flagellar agglutinins) encoded by the SAG1 (plus gametes and SAD1 (minus gametes) genes, activating a cAMP-dependent signaling pathway that ultimately leads to cell fusion and zygote formation. Our lab recently developed a Chlamydomonas strain expressing a SAG1-HA transgene, making it possible to study directly the properties of the SAG1 gene product. We found that SAG1 is cleaved soon after its synthesis to generate a C-terminal 65kDa fragment (SAG1-HA-C65), which we showed is an integral membrane protein. In my studies here, I describe new findings on the properties of SAG1-HA-C65 and its trafficking into and from the flagellar membrane. I found that in resting gametes SAG1-HA-C65 was distributed over the entire surface of the cell body plasma membrane, with little in the flagellar membrane indicating that SAG1-HA-C65 was excluded from the flagellar membrane. After flagellar adhesion-induced gamete activation or after activation of gametes with di-butyryl cAMP, SAG1-HA-C65 rapidly became enriched in the flagellar membrane concomitant with becoming concentrated around the bases of the flagella at the apical end of the cell. Moreover, cytoplasmic microtubules were required for SAG1-HA-C65 apical accumulation and for its significant delivery to the flagella during signaling. Apical concentration and flagellar enrichment of SAG1-HA-C65 occurred in cells conditionally depleted of the anterograde intraflagellar transport motor protein, kinesin-2. Furthermore, studies on the fate of SAG1-HA-C65 showed that it was shed into the medium in a detergent soluble membrane fraction during flagellar adhesion. This work provides new insights into membrane protein trafficking that regulates the protein composition of the ciliary/flagellar membrane. Advisors/Committee Members: Snell, William J..

Subjects/Keywords: Protein Transport; Flagella; Chlamydomonas

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APA (6^th Edition):

Hernandez Lara, C. I. (2013). Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hernandez Lara, Carmen Isabel. “Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 03, 2021. http://hdl.handle.net/2152.5/1217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hernandez Lara, Carmen Isabel. “Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling.” 2013. Web. 03 Mar 2021.

Vancouver:

Hernandez Lara CI. Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152.5/1217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hernandez Lara CI. Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

3. Wolff, Darold Melvin, 1940-. Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii.

Degree: PhD, General Science, 1971, Oregon State University

URL: http://hdl.handle.net/1957/45431

► The primary objective of this study was to develop methods for the selection of temperature sensitive division (TSD) mutants of Chlamydomonas reinhardtii. Such methods were… (more)

▼ The primary objective of this study was to develop methods for the selection of temperature sensitive division (TSD) mutants of Chlamydomonas reinhardtii. Such methods were developed and four temperature sensitive (TS) mutants were isolated and partially characterized. Wild type (WT) cells of the plus mating type were synchronized by a 12 hr light-12 hr dark regime at 25 C in culture tubes containing a high salt minimal medium buffered with PIPES (Piperazine-N, N'- bis (2-ethane sulfonic acid) monosodium monohydrate). Cells were mutagenized by irradiation with 1725 ergs/mm² of 254 nm ultraviolet light (UV) during the first half of the light period (G₁). The irradiated cells were kept in the dark for 24 hr to prevent photoreactivation, and then treated with a 10 mM concentration of hydroxyurea (HU) for 24 hr at an elevated temperature of 33 C. It was determined that this concentration of HU and this duration of treatment effectively kills WT cells at some stage i.n the middle of the generation cycle (presumably S phase). Temperature sensitive mutants that cannot progress into the HU-sensitive phase at 33 C were expected to survive. The combined treatment with UV and FU reduced the population to approximately 1% and increased the yield of possible TSD mutants. (UV alone reduced the population to 7% and HU to about 6.9%.) The cells were then plated on agar plates made with minimal medium and returned to 33 C for 5 days. The colonies that appeared during this time were considered to have arisen from unmutated cells or non-TSD mutants that had somehow survived the selection treatment; these colonies were marked and the agar plates placed at 25 C for an additional 5 days. The colonies that now formers were considered possible TSD mutants. Of 116 such colonies, only four were found to be temperature sensitive after replating and testing them at 33 and 25 C, the permissive temperature in this study. The TS mutants isolated were partially characterized on the basis of their responses to the restrictive temperature. The mutants, along with the WT cells, were observed in microdrops of minimal or complete medium covered with paraffin oil and on minimal or complete agar plates. The percentages of cells having divided once, twice, and three or more times were recorded at various times during continuous incubation at 25 C and during and after a 24 hr exposure to 33 C. In addition, the mutants were observed for viability and morphological changes. The effects of light intensities of 0, 200, and 600 foot candles were also studied. It was concluded that no ideal TSD mutant had been isolated. An ideal TSD mutant would be one in which division could be reversibly blocked by a restrictive temperature at a specific stage of the generation cycle. Although such mutants were not isolated, three of the four TS mutants (TSLt-1, TSLt-2, and TSGL) appear to show responses at the 33 C which suggest that some specific phase of the generation cycle is impaired, but the effect is not reversible and the… Advisors/Committee Members: Nachtwey, Stuart (advisor), Hisaw, Frederick L. (committee member).

Subjects/Keywords: Chlamydomonas reinhardii

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APA (6^th Edition):

Wolff, Darold Melvin, 1. (1971). Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/45431

Chicago Manual of Style (16^th Edition):

Wolff, Darold Melvin, 1940-. “Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii.” 1971. Doctoral Dissertation, Oregon State University. Accessed March 03, 2021. http://hdl.handle.net/1957/45431.

MLA Handbook (7^th Edition):

Wolff, Darold Melvin, 1940-. “Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii.” 1971. Web. 03 Mar 2021.

Vancouver:

Wolff, Darold Melvin 1. Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Oregon State University; 1971. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1957/45431.

Council of Science Editors:

Wolff, Darold Melvin 1. Isolation and partial characterization of temperature sensitive division mutants of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Oregon State University; 1971. Available from: http://hdl.handle.net/1957/45431

Michigan State University

4. Husic, Diane White. Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii.

Degree: PhD, Department of Biochemistry, 1986, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:24998

Subjects/Keywords: Chlamydomonas reinhardtii

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Husic, D. W. (1986). Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:24998

Chicago Manual of Style (16^th Edition):

Husic, Diane White. “Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii.” 1986. Doctoral Dissertation, Michigan State University. Accessed March 03, 2021. http://etd.lib.msu.edu/islandora/object/etd:24998.

MLA Handbook (7^th Edition):

Husic, Diane White. “Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii.” 1986. Web. 03 Mar 2021.

Vancouver:

Husic DW. Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Michigan State University; 1986. [cited 2021 Mar 03]. Available from: http://etd.lib.msu.edu/islandora/object/etd:24998.

Council of Science Editors:

Husic DW. Metabolism of D-lactate and structurally related organic acids in Chlamydomonas reinhardtii. [Doctoral Dissertation]. Michigan State University; 1986. Available from: http://etd.lib.msu.edu/islandora/object/etd:24998

The Ohio State University

5. Broderick, Lynne Sechrist. Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin.

Degree: PhD, Graduate School, 1969, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114

Subjects/Keywords: Biology; Chlamydomonas

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APA (6^th Edition):

Broderick, L. S. (1969). Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114

Chicago Manual of Style (16^th Edition):

Broderick, Lynne Sechrist. “Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin.” 1969. Doctoral Dissertation, The Ohio State University. Accessed March 03, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114.

MLA Handbook (7^th Edition):

Broderick, Lynne Sechrist. “Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin.” 1969. Web. 03 Mar 2021.

Vancouver:

Broderick LS. Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin. [Internet] [Doctoral dissertation]. The Ohio State University; 1969. [cited 2021 Mar 03]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114.

Council of Science Editors:

Broderick LS. Light and electron microscopy of vegetative cells and developing zygotes of isolated strains of chlamydomonas mexicana lewin. [Doctoral Dissertation]. The Ohio State University; 1969. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486652767114114

University of Tasmania

6. Vucica, Yvonne. Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum.

Degree: 2002, University of Tasmania

URL: https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf

Subjects/Keywords: Flagella (Microbiology); Chlamydomonas; Chlamydomonas reinhardtii

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vucica, Y. (2002). Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vucica, Yvonne. “Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum.” 2002. Thesis, University of Tasmania. Accessed March 03, 2021. https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vucica, Yvonne. “Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum.” 2002. Web. 03 Mar 2021.

Vancouver:

Vucica Y. Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum. [Internet] [Thesis]. University of Tasmania; 2002. [cited 2021 Mar 03]. Available from: https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vucica Y. Insertional mutants of Chlamydomonas affecting the central pair apparatus of the flagellum. [Thesis]. University of Tasmania; 2002. Available from: https://eprints.utas.edu.au/22090/1/whole_VucicaYvonne2002_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

7. Malnoë, Alizée. A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii.

Degree: Docteur es, Biologie, 2011, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2011PA112359

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Le cytochrome b6f est un complexe majeur de la chaîne photosynthétique oxygénique de par son activité quinol:plastocyanine oxydoréductase, qui contribue à la formation d’ATP via… (more)

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Le cytochrome b6f est un complexe majeur de la chaîne photosynthétique oxygénique de par son activité quinol:plastocyanine oxydoréductase, qui contribue à la formation d’ATP via un transfert d’électrons couplé à un transfert de protons. La présence d’un hème c particulier lié par une seule liaison covalente, l’hème ci, au sein du site de réduction de quinone Qi du cytochrome b6f constitue une différence notable en comparaison avec son homologue de la chaîne respiratoire, le cytochrome bc1. Un cytochrome b6f dépourvu d’hème ci est dégradé, sa faible accumulation ne permet pas une croissance photosynthétique. Cette observation a donné lieu à une recherche de suppresseurs permettant une plus grande accumulation de cytochrome b6f dont la fonction même altérée, serait suffisante pour assurer une croissance photosynthétique. Cette approche génétique de recherche de suppresseur a été entreprise chez Chlamydomonas reinhardtii. Ce travail de thèse a permis l’isolation et la caractérisation d’un mutant de la protéase FtsH1 (mutation R420C qui affecterait l’activité ATPasique). Le mutant ftsh1-1 s’est révélé être un outil puissant pour l’étude fonctionnelle de complexes mutés autrement dégradés. Une approche multidisciplinaire combinant expériences de génétique, biochimie, physiologie et biophysique a démontré notamment que : (i) le mutant QiKO, dont le complexe b6f est dépourvu des hèmes bh et ci, peut pousser de manière phototrophique malgré un Q-cycle cassé, (ii) l’absence d’hème ci lié covalemment, pour le mutant Rccb2, génère une photosensibilité exacerbée en présence d’oxygène, ce qui sous-tend un rôle pour l’hème ci dans un environnement riche en oxygène, (iii) la protéase FtsH exerce un contrôle qualité global des complexes majeurs photosynthétiques.

Central in oxygenic photosynthesis, the cytochrome b6f complex, couples electron transfer to proton translocation across the thylakoid membrane via its quinol:plastocyanin oxidoreductase activity, contributing to ATP formation. Cytochrome b6f complex differs from its respiratory homolog, the bc1 complex, by the presence of an additional heme, heme ci located within the quinone reduction site Qi and attached by a unique thioether bond. Mutants lacking heme ci show low accumulation of partially functional b6f complex and, hence, cannot grow phototrophically. This grounded a screen for suppressor mutations that would restore higher accumulation of b6f complexes whose function, even if compromised, would sustain phototrophic growth.The genetic suppressor approach undertook in Chlamydomonas reinhardtii during this PhD thesis led to the isolation and characterisation of the ftsh1-1 protease mutant (mutation R420C which should affect ATP hydrolysis). The mutant ftsh1-1 proved to be a versatile tool for the functional study of otherwise degraded proteins. The combination of genetic, biochemical, physiological and biophysical experiments demonstrated notably that: (i) a QiKO mutant, whose b6f complexes are devoid of both bh and ci hemes, can grow phototrophically despite a broken…

Advisors/Committee Members: Vitry, Catherine de (thesis director).

Subjects/Keywords: Photosynthèse; Cytochrome; Protéase; Chlamydomonas; Photosynthesis; Cytochrome; Protease; Chlamydomonas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Malnoë, A. (2011). A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2011PA112359

Chicago Manual of Style (16^th Edition):

Malnoë, Alizée. “A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii.” 2011. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 03, 2021. http://www.theses.fr/2011PA112359.

MLA Handbook (7^th Edition):

Malnoë, Alizée. “A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii.” 2011. Web. 03 Mar 2021.

Vancouver:

Malnoë A. A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2011. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2011PA112359.

Council of Science Editors:

Malnoë A. A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii : Une approche génétique de recherche de suppresseur pour l’étude de la biogenèse, du contrôle qualité et de la fonction des complexes photosynthétiques chez Chlamydomonas reinhardtii. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2011. Available from: http://www.theses.fr/2011PA112359

8. Clowez, Sophie. Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii.

Degree: Docteur es, Biophysique, 2014, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2014PA066525

►

Le processus photosynthétique se traduit par un flux d’électron impliquant différents complexes de la membrane thylacoïdale. Ce flux peut adopter deux chemins différents : le… (more)

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Le processus photosynthétique se traduit par un flux d’électron impliquant différents complexes de la membrane thylacoïdale. Ce flux peut adopter deux chemins différents : le transfert d’électron linéaire (Merchant, Prochnik et al. 2007) à travers lequel les électrons sont transférés de l’eau oxydée au niveau du Photosystème II (PSII), au NADPH réduit par le PSI ; et le transfert d’électron cyclique autour du Photosystème I (PSI) et du complexe cytochrome b6f. Ces flux d’électrons sont couplés à un pompage de proton du stroma vers le lumen générant une différence de potentiel transmembranaire, permettant la synthèse d’ATP (Allen 2002). La coexistence de ces deux flux est considéré comme nécessaire à la fixation et la métabolisation des molécules de dioxyde de carbone (Seelert, Poetsch et al. 2000 ; Munekage, Hashimoto et al. 2004) dans un rapport stricte ATP / NADPH. Cette coexistence qui semble essentiel soulève la question des mécanismes qui prévalent à l’implication des mêmes acteurs photosynthétiques, dans une même membrane, dans l’un ou l’autre mode de transfert d’électron. Chez l’algue verte Chlamydomonas reinhardtii, nous avons démontré que la commutation entre les deux transferts était dépendante de l’état redox des cellules, mais contrairement à ce qui avait été suggéré dans les études précédentes (Bulté, Rebeillé et al. 1990 ; Finazzi, Rappaport et al. 2002) indépendante du phénomène de transition d’état (Takahashi, Clowez et al. 2013), qui implique la migration latérale des complexes antennaires au sein de la membrane. L’association de ces antennes au Photosystème I conduirait à la séquestration, dans une même entité biochimique, des différents acteurs du mode cyclique. Cette formation de supercomplexe dans les conditions anoxiques, à fait l’objet d’une étude fonctionnelle in vitro, laissant quelques questions ouvertes sur leurs capacités fonctionnelles. Ce travail de thèse présente aussi la caractérisation d’une limitation transitoire des accepteurs du Photosystème I, en début d’anoxie pendant laquelle il n’est pas possible d’observer d’oxydation de P700, à 705 nm. Ce phénomène dû à la recombinaison de charge est créé par un engorgement du pool de NADPH. L’oxydation spontanée du PSI au bout d’un certain temps d’anoxie implique l’induction de l’hydrogénase, acceptant les électrons du PSI. Il reste possible d’induire cette évolution de l’oxydation de P700 lorsque les cellules sont constamment sous illumination dans les conditions anoxiques, impliquant cette fois ci, la voie de l’ATP chloroplastique. L’ATP synthétisé à la lumière permettrait la consommation de NADPH via le cycle de Benson Calvin.

The photosynthetic process relies on an electron flow involving several complexes in the thylakoid membranes of photosynthetic organisms. This flux can follow two possibly competing pathways: the linear electron transfer through which electrons are transferred from water (which is oxidized) to NADP+ (which is reduced), which is coupled to the generation of a transmembrane potential difference allowing the…

Advisors/Committee Members: Rappaport, Fabrice (thesis director).

Subjects/Keywords: Photosynthèse; Électron; Transfert; Spectrophotométrie; Métabolisme; Chlamydomonas; Photosynthesis; Electron; Chlamydomonas reinhardtii; 572.46

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clowez, S. (2014). Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2014PA066525

Chicago Manual of Style (16^th Edition):

Clowez, Sophie. “Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii.” 2014. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed March 03, 2021. http://www.theses.fr/2014PA066525.

MLA Handbook (7^th Edition):

Clowez, Sophie. “Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii.” 2014. Web. 03 Mar 2021.

Vancouver:

Clowez S. Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2014PA066525.

Council of Science Editors:

Clowez S. Conséquences Fonctionnelles de l’Organisation Supramoléculaire de la Chaîne Photosynthétique et Commutation Entre Transferts d’Electrons Cyclique et Linéaire : Functional consequences of thylakoid membranes reorganization in photosynthetic chain and switching between cyclic and linear electron transfer in green algae Chlamydomonas reinhardtii. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. Available from: http://www.theses.fr/2014PA066525

Ruhr Universität Bochum

9. Lambertz, Camilla Regina. Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii.

Degree: 2010, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037

► Das Ferredoxin-kodierende Gen FDX5 aus C. reinhardtii fällt durch einen besonders starken Expressionsanstieg unter Sauerstoff- und Kupfermangel auf. Über Reportergenanalysen konnten zwei GTAC-Motive für die… (more)

Subjects/Keywords: Chlamydomonas reinhardtii; Hydrogenase; Transkriptionsfaktor; Hypoxie; Anaerobiose

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lambertz, C. R. (2010). Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lambertz, Camilla Regina. “Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii.” 2010. Thesis, Ruhr Universität Bochum. Accessed March 03, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lambertz, Camilla Regina. “Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii.” 2010. Web. 03 Mar 2021.

Vancouver:

Lambertz CR. Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii. [Internet] [Thesis]. Ruhr Universität Bochum; 2010. [cited 2021 Mar 03]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lambertz CR. Untersuchungen zur Regulation anaerob exprimierter Gene aus Chlamydomonas reinhardtii. [Thesis]. Ruhr Universität Bochum; 2010. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-29037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

10. Pizzoli, Guilherme. Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii.

Degree: 2016, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/170066

►

Considerada a mais comum das doenças lisossômicas, a doença de Gaucher é causada por mutações no gene GBA1 que resultam na síntese defeituosa da enzima… (more)

▼

Considerada a mais comum das doenças lisossômicas, a doença de Gaucher é causada por mutações no gene GBA1 que resultam na síntese defeituosa da enzima glicocerebrosidase (GBA), responsável pela hidrólise dos glicocerebrosídios em glicose e ceramida. A deficiência da enzima provoca o acúmulo desses glicolipídios nos macrófagos, principalmente no fígado, no baço e na medula óssea, levando a um fenótipo complexo. O tratamento da doença consiste na administração da enzima GBA humana (HsGBA) exógena e, apesar de sua eficácia, é extremamente oneroso. Assim como outras espécies de microalgas, Chlamydomonas reinhardtii apresenta alto potencial para a produção de grandes quantidades de proteínas recombinantes de forma rápida e a um custo muito inferior ao dos sistemas de expressão tradicionais. O objetivo proposto para o desenvolvimento deste trabalho foi a expressão do gene codificador da HsGBA a partir do genoma nuclear de C. reinhardtii visando à produção da proteína como possível alternativa para a terapia de reposição enzimática. O gene HsGBA artificial foi projetado com códons adaptados para a expressão nuclear em C. reinhardtii e com sítios de restrição. A sequência nucleotídica foi sintetizada pela empresa GenScript USA Inc. e, após seu recebimento em plasmídeo de clonagem, o gene HsGBA foi inserido no plasmídeo pHsp70A/RbcS2-cgLuc por restrição com endonucleases e ligação. Esse vetor foi combinado com o plasmídeo pKS-aph7``-lox para a transferência do cassete de expressão do gene de interesse por recombinação sítio específica mediada pelo sistema Cre/lox, originando o vetor pKS-aph7``-lox::HsGBA, que contém, assim, os cassetes de expressão em tandem para HsGBA e para o marcador de seleção aph7``, codificador da enzima aminoglicosídeo fosfotransferase e capaz de conferir resistência à higromicina B. A linhagem de C. reinhardtii CC-400 cw15 mt+ foi transformada por eletroporação com o plasmídeo resultante linearizado (clivado com EcoRV) e na forma circular. A integração de HsGBA no genoma de cinco linhagens de C. reinhardtii foi comprovada por PCR e sua expressão foi demonstrada em três dessas linhagens de forma qualitativa por RT-PCR. Como HsGBA é controlado por um promotor induzível, hsp70A, diversas condições foram testadas visando à sua máxima expressão. Entretanto, análises por SDS-PAGE e western blot não permitiram a detecção da proteína recombinante. De modo semelhante, a atividade enzimática da HsGBA avaliada em extratos proteicos de linhagens transformadas não foi diferente da observada para linhagens não transformadas de C. reinhardtii.

Considered the most common lysosomal disorder, Gaucher disease is caused by mutations in GBA1 gene that result in defective synthesis of the enzyme glucocerebrosidase (GBA), responsible for the hydrolysis of glucocerebrosides into glucose and ceramide. When the enzyme is defective, these glycolipids accumulate in the macrophages, mainly in the liver, spleen and bone marrow, leading to a complex phenotype. Current treatment consists of enzyme replacement therapy by the…

Advisors/Committee Members: Pasquali, Giancarlo.

Subjects/Keywords: Glicocerebrosidase; Chlamydomonas reinhardtii; Doença de Gaucher

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pizzoli, G. (2016). Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/170066

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pizzoli, Guilherme. “Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii.” 2016. Thesis, Universidade do Rio Grande do Sul. Accessed March 03, 2021. http://hdl.handle.net/10183/170066.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pizzoli, Guilherme. “Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii.” 2016. Web. 03 Mar 2021.

Vancouver:

Pizzoli G. Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10183/170066.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pizzoli G. Expressão do gene da glicocerebrosidase humana em Chlamydomonas reinhardtii. [Thesis]. Universidade do Rio Grande do Sul; 2016. Available from: http://hdl.handle.net/10183/170066

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Oh, Victor Hyun. EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/37085

► Green eukaryotic microalgae are an attractive economic, and efficient, alternative to conventional recombinant protein expression systems and have the potential to revolutionize the biotechnology industry.… (more)

▼ Green eukaryotic microalgae are an attractive economic, and efficient, alternative to conventional recombinant protein expression systems and have the potential to revolutionize the biotechnology industry. Recent advancements in the genomic and proteomic techniques of microalgae have ushered tremendous efforts to optimize the “upstream” side of process design through the use of promoters, codon-optimization, and plasmid design. While the prospective for microalgae as a molecular farming platform is high, there are still a number of major obstacles, namely inconsistent transgene expression, that must be overcome before it can be implemented as a standard expression system. The supplementation of culture medium with sodium butyrate to enhance transgene expression in mammalian cells for higher protein yields has been studied for several decades. Despite the wealth of literature available on the role of sodium butyrate in inducing changes of the morphology, growth rate, and gene expression in mammalian and plant cells, there is little information documented on its effect on green microalgal cells. In this study we explore the changes in cell morphology, cell growth, and transgene expression of a recombinant anti-apoptotic Venus-Bcl-xL protein in microalgae Chlamydomonas reinhardtii (C. reinhardtii) cells induced by sodium butyrate supplementation, and investigate the prospective of combining the advantages of a genetically modified anti-apoptotic C. reinhardtii strain with the transgene enhancing effects of sodium butyrate. We observed that concentrations of sodium butyrate above 250 mM were lethal for both the wild-type C. reinhardtii wall-deficient (CW 15+) UTEX 2337 strain and C. reinhardtii wall-deficient Venus-Bcl-xL (pRelax) strain. Concentrations of 50 mM and 100 mM induced phonotypical changes in the wild-type C. reinhardtii cell morphology induced apoptosis and inhibited cell growth. The difference in final cell density for cultures induced with 50 mM and 100 mM sodium butyrate from untreated cells averaged 5.79% and 62.92%, respectively. The anti-apoptotic pRelax C. reinhardtii strain showed significant signs of tolerance to apoptosis and cell growth inhibition at 50 mM and 100 mM sodium butyrate as cultures treated with 50 mM and 100 mM sodium butyrate were 75% and 78% less apoptotic than untreated pRelax cells, respectively, and difference in final cell density from untreated pRelax cells were -13.08% and 1.675%, respectively. The transgenic pRelax strain induced with 50 mM and 100 mM sodium butyrate showed substantial reduction in apoptotic behavior compared to untreated pRelax cells, indicating the possibility of up-regulation of the recombinant anti-apoptotic Venus-Bcl-xL protein caused by sodium butyrate induction. Advisors/Committee Members: Frachette, Joelle (advisor).

Subjects/Keywords: sodium butyrate; chlamydomonas; recombinant; bcl-xL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oh, V. H. (2014). EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37085

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oh, Victor Hyun. “EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII.” 2014. Thesis, Johns Hopkins University. Accessed March 03, 2021. http://jhir.library.jhu.edu/handle/1774.2/37085.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oh, Victor Hyun. “EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII.” 2014. Web. 03 Mar 2021.

Vancouver:

Oh VH. EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Mar 03]. Available from: http://jhir.library.jhu.edu/handle/1774.2/37085.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oh VH. EXPLORING THE EFFECTS OF SODIUM BUTYRATE ON CELL MORPHOLOGY, CELL GROWTH, AND EXPRESSION OF A RECOMBINANT ANTI-APOPTOTIC VENUS–BCL-XL FUSION PROTEIN IN GREEN MICROALGAE CHLAMYDOMONAS REINHARDTII. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37085

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

12. McSpadden, Ethan DeNardo. Structural and evolutionary studies on CaMKII oligomerization.

Degree: Molecular & Cell Biology, 2018, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/00k0r2pt

► Calcium-calmodulin-dependent protein kinase II (CaMKII) plays a critical role in animal learning and memory (Giese et al. 1998). CaMKII operates as a multi-subunit holoenzyme and… (more)

▼ Calcium-calmodulin-dependent protein kinase II (CaMKII) plays a critical role in animal learning and memory (Giese et al. 1998). CaMKII operates as a multi-subunit holoenzyme and can detect the frequency of incident Ca2+ pulse trains entering the cell (Chao et al. 2011; De Koninck and Schulman 1998). CaMKII holoenzymes become destabilized and appear to exchange subunits following catalytic activation, which results in the spread of kinase activity to inactive holoenzymes (Stratton et al. 2014; Bhattacharyya et al. 2016). This phenomenon is not fully understood and is under active investigation.This work focuses on the structural and biochemical characterization of CaMKII hub domain assemblies, which are essential to holoenzyme formation (Shen and Meyer 1998; Kolb et al. 1998). The crystal structure of a dodecameric human CaMKII-α hub domain assembly shows how the protein may be able to sense the activation state of the CaMKII kinase domain. This helps explain why kinase activation destabilizes CaMKII holoenzymes and promotes subunit exchange.The characterization of CaMKII hub domains from evolutionarily divergent organisms is also presented. Hub domains encoded by three related green algae assemble into 16-, 18-, and 20-subunit oligomers. These are the largest known CaMKII hub domain assemblies. A crystal structure of one, the Chlamydomonas reinhardtii 18-mer, revealed multiple intra-chain hydrogen bonds not present in the human isoform. When these hydrogen bonds were engineered into the human CaMKII hub domain by mutation the protein oligomerized into larger assemblies. A larger CaMKII holoenzyme is predicted to be more resistant to deactivation by protein phosphatases and thus may be useful in studying the consequences of aberrant CaMKII activity. These hydrogen bonds are also predicted to inhibit subunit exchange in human CaMKII by dampening structural fluctuations in the hub domain.Work is also described on an interfacial mutation in the hub domain that weakens the integrity of the CaMKII holoenzyme. CaMKII dimers are present in solution as a result of this mutation. Catalytic activation increases the dimer population, demonstrating that the hub domain interface weakened by the mutation is further destabilized when CaMKII is active. This suggests that dimer release is a central component of activation-triggered subunit exchange between CaMKII holoenzymes.

Subjects/Keywords: Biochemistry; CaMKII; Chlamydomonas; Human; Protein; Structure

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McSpadden, E. D. (2018). Structural and evolutionary studies on CaMKII oligomerization. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/00k0r2pt

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McSpadden, Ethan DeNardo. “Structural and evolutionary studies on CaMKII oligomerization.” 2018. Thesis, University of California – Berkeley. Accessed March 03, 2021. http://www.escholarship.org/uc/item/00k0r2pt.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McSpadden, Ethan DeNardo. “Structural and evolutionary studies on CaMKII oligomerization.” 2018. Web. 03 Mar 2021.

Vancouver:

McSpadden ED. Structural and evolutionary studies on CaMKII oligomerization. [Internet] [Thesis]. University of California – Berkeley; 2018. [cited 2021 Mar 03]. Available from: http://www.escholarship.org/uc/item/00k0r2pt.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McSpadden ED. Structural and evolutionary studies on CaMKII oligomerization. [Thesis]. University of California – Berkeley; 2018. Available from: http://www.escholarship.org/uc/item/00k0r2pt

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

13. Belzile, Olivier. The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization.

Degree: 2012, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/984

► The cellular and molecular mechanisms specifying the membrane protein composition of cilia and flagella at steady state and during cilium-generated signaling are poorly understood. Our… (more)

▼ The cellular and molecular mechanisms specifying the membrane protein composition of cilia and flagella at steady state and during cilium-generated signaling are poorly understood. Our laboratory uses the biflagellated green alga Chlamydomonas as a model system to study regulated movement of the flagellar adhesion receptor SAG1 from the cell body to the flagella. Interactions between the plus flagellar receptor (agglutinin) SAG1 and its cognate receptor SAD1 on flagella of minus gametes induces flagellar adhesion and activation of a cAMP-dependent signaling pathway ultimately leading to cell-cell fusion. Although previous work from our laboratory and others suggested that pathway activation triggers mobilization of a pool of SAG1 from the cell body to the flagella, those studies depended on indirect adhesion bioassays detecting the activity of SAG1, not the protein. Here, I report use of new tools to study directly the regulation of SAG1 localization. I show that the SAG1 gene bearing a C-terminal HA tag rescues flagellar adhesion and cell fusion in the flagellar adhesion mutant, sag1-5. Immunofluorescence studies of resting SAG1-HA/sag1-5 mt+ plus gametes show that the protein is present mostly on cell bodies. Biochemical studies show that only gametes express SAG1-HA. Detection of precursor forms indicates SAG1 undergoes cleavage soon after its synthesis to yield an HA-tagged 65 kDa, C-terminal portion (SAG1-HA-C65), and that SAG1-HA-C65 is on the cell surface. Consistent with the predicted 3 transmembrane domains at the C-terminus, release of SAG1-HA-C65 in a soluble form requires detergent and is not achieved upon mechanical disruption, high salt, or high pH treatments. Cell fractionation demonstrates that in resting gametes the majority of SAG1-HA-C65 is present on cell bodies and only a small amount is on flagella. Within minutes after signaling is triggered by flagellar adhesion, however, SAG1-HA-C65 is mobilized from the cell body to the flagella and the organelles become highly enriched in the protein, where it forms large detergent-resistant complexes. I show that Chlamydomonas can regulate the amount of SAG1 in the flagella through cilium-generated signaling, and thus provides the first system for studying regulation of the membrane protein composition of the cilium/flagellum in a biochemically tractable system. Advisors/Committee Members: Snell, William J..

Subjects/Keywords: Cell Surface Extensions; Protozoan Proteins; Chlamydomonas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Belzile, O. (2012). The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/984

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Belzile, Olivier. “The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 03, 2021. http://hdl.handle.net/2152.5/984.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Belzile, Olivier. “The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization.” 2012. Web. 03 Mar 2021.

Vancouver:

Belzile O. The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152.5/984.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belzile O. The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/984

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Chicago

14. Elgass, Laura Jane. Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments.

Degree: 2014, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/19156

► Microfluidic platforms are excellent tools for studying the effects of precisely controlled oxygen and other gas environments on various biological systems, from mammalian cell culture… (more)

Subjects/Keywords: microfluidics; chlamydomonas reinhardtii; volvox carteri; oxygen control

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Elgass, L. J. (2014). Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/19156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elgass, Laura Jane. “Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments.” 2014. Thesis, University of Illinois – Chicago. Accessed March 03, 2021. http://hdl.handle.net/10027/19156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elgass, Laura Jane. “Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments.” 2014. Web. 03 Mar 2021.

Vancouver:

Elgass LJ. Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments. [Internet] [Thesis]. University of Illinois – Chicago; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10027/19156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elgass LJ. Microfluidic Approaches for Algae Culture in Controlled Oxygen Environments. [Thesis]. University of Illinois – Chicago; 2014. Available from: http://hdl.handle.net/10027/19156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University College London (University of London)

15. Lown, Felicity Jane. Respiratory mutants of Chlamydomonas.

Degree: PhD, 2001, University College London (University of London)

URL: https://discovery.ucl.ac.uk/id/eprint/10100488/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271247

► The aim of the work described in this thesis was to investigate the role the nuclear genome plays in the biogenesis of the respiratory chain… (more)

Subjects/Keywords: 580; Chlamydomonas reinhardtii

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APA (6^th Edition):

Lown, F. J. (2001). Respiratory mutants of Chlamydomonas. (Doctoral Dissertation). University College London (University of London). Retrieved from https://discovery.ucl.ac.uk/id/eprint/10100488/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271247

Chicago Manual of Style (16^th Edition):

Lown, Felicity Jane. “Respiratory mutants of Chlamydomonas.” 2001. Doctoral Dissertation, University College London (University of London). Accessed March 03, 2021. https://discovery.ucl.ac.uk/id/eprint/10100488/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271247.

MLA Handbook (7^th Edition):

Lown, Felicity Jane. “Respiratory mutants of Chlamydomonas.” 2001. Web. 03 Mar 2021.

Vancouver:

Lown FJ. Respiratory mutants of Chlamydomonas. [Internet] [Doctoral dissertation]. University College London (University of London); 2001. [cited 2021 Mar 03]. Available from: https://discovery.ucl.ac.uk/id/eprint/10100488/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271247.

Council of Science Editors:

Lown FJ. Respiratory mutants of Chlamydomonas. [Doctoral Dissertation]. University College London (University of London); 2001. Available from: https://discovery.ucl.ac.uk/id/eprint/10100488/ ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271247

Colorado State University

16. Link, Alicia. Alternative splicing and its regulatory mechanisms in photosynthetic eukaryotes.

Degree: MS(M.S.), Biology, 2011, Colorado State University

URL: http://hdl.handle.net/10217/70809

► In recent years, alternative splicing (AS) of pre-mRNAs, which generates multiple transcripts from a single gene, has emerged as an important process in general proteome… (more)

▼ In recent years, alternative splicing (AS) of pre-mRNAs, which generates multiple transcripts from a single gene, has emerged as an important process in general proteome diversity and in regulatory gene expression in multicellular eukaryotes. In Arabidopsis over 40% of intron-containing genes are alternatively spliced. However, mechanisms by which AS is regulated in plants are not fully understood, primarily due to the lack of an in vitro splicing system derived from plants. Furthermore, the extent of AS in simple unicellular photosynthetic eukaryotes from which plants have evolved is also not known. My research addresses these two attributes of splicing in plants. In Part 1 of my thesis, I have investigated an aspect of AS regulation in plants. We have previously shown that an SR-related splicing regulator called SR45 regulates AS of pre-mRNAs in Arabidopsis by altering splice site selection (Ali et al. 2007). In this work using bimolecular fluorescent complements, I have demonstrated that SR45 interacts with U2AF35, an important spliceosomal protein involved in 3' splice site selection in plant cells. This interaction takes place in the nucleus, specifically in the subnuclear domains called speckles, which are known to contain splicing regulators and other proteins involved in transcription. My work has shown that SR45 interacts with both paralogs of U2AF35 and I mapped the domains in SR45 that are involved in its interaction with U2AF35. In addition, my studies have revealed interaction of the paralogs as hetero- and homodimers. Interestingly, U2AF35 was found to interact with U1-70K, a key protein involved in 5' splice site selection. Based on this work and previous work in our laboratory, a model is proposed that explains the role of SR45 in splice site selection. In the second part of my work I studied the extent of alternative splicing (AS) in the unicellular green alga Chlamydomonas, that shares a common ancestor with land plants. In collaboration with Dr. Asa Ben Hur's lab, we have performed a comprehensive analysis of AS in Chlamydomonas reinhardtii using both computational and experimental methods. Our results show that AS is common in Chlamydomonas, but its extent is less than what is observed in land plants. However, the relative frequency of different splicing events in Chlamydomonas is very similar to higher plants. We have found that a large number of genes undergo alternative splicing, and together with the simplicity of the system and the use of available molecular and genetic tools. This organism is an experimental system to investigate the mechanisms involved in alternative splicing. To further validate predicted splice variants, we performed extensive analysis of AS for two genes, which not only confirmed predictions but also revealed novel splice variants, suggesting that the extent of AS is higher than we predicted. AS can also play a role in the regulation of gene expression through processes such as regulated unproductive splicing and translation (RUST) that involves nonsense-mediated decay… Advisors/Committee Members: Reddy, A. S. N. (advisor), Stack, Stephen (committee member), Lapitan, Nora (committee member).

Subjects/Keywords: algae; alternative splicing; NMD; SR45; U2AFs; Chlamydomonas

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APA (6^th Edition):

Link, A. (2011). Alternative splicing and its regulatory mechanisms in photosynthetic eukaryotes. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/70809

Chicago Manual of Style (16^th Edition):

Link, Alicia. “Alternative splicing and its regulatory mechanisms in photosynthetic eukaryotes.” 2011. Masters Thesis, Colorado State University. Accessed March 03, 2021. http://hdl.handle.net/10217/70809.

MLA Handbook (7^th Edition):

Link, Alicia. “Alternative splicing and its regulatory mechanisms in photosynthetic eukaryotes.” 2011. Web. 03 Mar 2021.

Vancouver:

Link A. Alternative splicing and its regulatory mechanisms in photosynthetic eukaryotes. [Internet] [Masters thesis]. Colorado State University; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10217/70809.

Council of Science Editors:

Link A. Alternative splicing and its regulatory mechanisms in photosynthetic eukaryotes. [Masters Thesis]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/70809

University of Cambridge

17. Meyer, Moritz. Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid.

Degree: PhD, 2010, University of Cambridge

URL: http://www.dspace.cam.ac.uk/handle/1810/226160https://www.repository.cam.ac.uk/bitstream/1810/226160/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/5/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/3/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/6/thesis.pdf.jpg

► Aquatic photosynthesis accounts for 50% of the global annual net primary production (NPP), despite frequent low availability and limited diffusion of CO2 in the aquatic… (more)

▼ Aquatic photosynthesis accounts for 50% of the global annual net primary production (NPP), despite frequent low availability and limited diffusion of CO2 in the aquatic milieu, and low affinity for CO2 by the primary carboxylating enzyme, Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Many eukaryotic algae, and a single group of land plants, the hornworts, have an inducible carbon concentrating mechanism (CCM), to overcome these limitations. The efficiency of the CCM is improved when RuBisCO is localised to a subcellular compartment, the pyrenoid, which is hypothesised to act as a diffusion barrier for CO2 . Although the pyrenoid is a major player in global carbon balance (we estimate 10-15% of NPP), it is one of the few remaining prominent cellular features without a precise molecular or physiological definition. Under ambient CO2 , at least 90% of the cellular RuBisCO is packed into a dense matrix, together with the chaperone RuBisCO activase. Thylakoid membranes usually traverse the pyrenoid matrix, and the carboxylating substrate is thought to be delivered to the active sites of the enzyme via a carbonic anhydrase located in the lumen of these thylakoids. The mechanism of aggregation of constituents within the pyrenoid, however, still remains largely unknown. Comprehensive mutant screens have yet to reveal mutants incapable of forming pyrenoids other than those mutants with a defective RuBisCO holoenzyme, whereas DNA microarray studies uncovered little with reference to pyrenoid ultrastructure or aggregation. Taken together, this evidence raises the possibility that the basis of pyrenoid ultrastructure and aggregation lies entirely in sequence variations of RuBisCO itself. This work explored, firstly, the advantages conferred by an active CCM in hornworts and in unicellular algae, compared with the passive CO2 acquisition in most terrestrial plants. A physiological framework to CCM and pyrenoid-based photosynthesis, and isotopic discrimination, was provided by comparing the photosynthetic characteristics of selected bryophytes and algae, differing in chloroplast morphology and degrees of internalisation of gas exchanges. The results showed that on-line, carbon isotope discrimination values were a good indicator of CCM occurrence, as well as liquid-phase diffusion limitation, and biochemical limitations resulting from declining RuBisCO activity and electron transport. The methodology was used to diagnose the presence of an active CCM, and the extent of CO2 leakage. Secondly, the effect of RuBisCO sequence variations on the pyrenoid, and associated CCM, was studied using the model alga Chlamydomonas reinhardtii. The starting premise was the report by Nozaki et al. (2002) that, in some species of the family Chlamydomonaceae, a few amino acid residues within the RuBisCO large subunit (LSU) correlated strongly with pyrenoid formation. The specific roles of seven LSU residues were studied by site-directed mutagenesis. Whilst the mutations reduced the affinity of RuBisCO for CO2 and increased CO2 leakage,…

Subjects/Keywords: Pyrenoid; Rubisco; Carbon concentrating mechanism; Chlamydomonas; RbcS

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APA (6^th Edition):

Meyer, M. (2010). Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid. (Doctoral Dissertation). University of Cambridge. Retrieved from http://www.dspace.cam.ac.uk/handle/1810/226160https://www.repository.cam.ac.uk/bitstream/1810/226160/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/5/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/3/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/6/thesis.pdf.jpg

Chicago Manual of Style (16^th Edition):

Meyer, Moritz. “Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid.” 2010. Doctoral Dissertation, University of Cambridge. Accessed March 03, 2021. http://www.dspace.cam.ac.uk/handle/1810/226160https://www.repository.cam.ac.uk/bitstream/1810/226160/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/5/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/3/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/6/thesis.pdf.jpg.

MLA Handbook (7^th Edition):

Meyer, Moritz. “Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid.” 2010. Web. 03 Mar 2021.

Vancouver:

Meyer M. Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid. [Internet] [Doctoral dissertation]. University of Cambridge; 2010. [cited 2021 Mar 03]. Available from: http://www.dspace.cam.ac.uk/handle/1810/226160https://www.repository.cam.ac.uk/bitstream/1810/226160/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/5/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/3/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/6/thesis.pdf.jpg.

Council of Science Editors:

Meyer M. Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid. [Doctoral Dissertation]. University of Cambridge; 2010. Available from: http://www.dspace.cam.ac.uk/handle/1810/226160https://www.repository.cam.ac.uk/bitstream/1810/226160/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/5/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/3/thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226160/6/thesis.pdf.jpg

18. Mera, Roi. El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff .

Degree: 2016, Universidad da Coruña

URL: http://hdl.handle.net/2183/16919

► [Resumen] El sulfato es la principal fuente de azufre para las microalgas y, por lo tanto, un nutriente esencial para estos microorganismos. El conocimiento de… (more)

Subjects/Keywords: Chlamydomonas moewusii; Microalgas-Crecimiento; Cadmio-Toxicología

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APA (6^th Edition):

Mera, R. (2016). El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff . (Doctoral Dissertation). Universidad da Coruña. Retrieved from http://hdl.handle.net/2183/16919

Chicago Manual of Style (16^th Edition):

Mera, Roi. “El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff .” 2016. Doctoral Dissertation, Universidad da Coruña. Accessed March 03, 2021. http://hdl.handle.net/2183/16919.

MLA Handbook (7^th Edition):

Mera, Roi. “El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff .” 2016. Web. 03 Mar 2021.

Vancouver:

Mera R. El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff . [Internet] [Doctoral dissertation]. Universidad da Coruña; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2183/16919.

Council of Science Editors:

Mera R. El sulfato como nutriente esencial en la protección contra los efectos citotóxicos del cadmio en la microalga dulceacuícula "Chlamydomonas moewussii" Gerloff . [Doctoral Dissertation]. Universidad da Coruña; 2016. Available from: http://hdl.handle.net/2183/16919

University of Minnesota

19. VanderWaal Mills, Kristyn E. Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii.

Degree: PhD, Molecular, Cellular, Developmental Biology and Genetics, 2011, University of Minnesota

URL: http://hdl.handle.net/11299/165777

► Cilia and flagella are microtubule-based organelles that perform critical functions in human health and development. The I1 inner arm dynein and the IC138 subunit play… (more)

▼ Cilia and flagella are microtubule-based organelles that perform critical functions in human health and development. The I1 inner arm dynein and the IC138 subunit play a key role in the regulation of flagellar motility. To understand how the IC138 protein and its associated subunits modulate I1 activity, we characterized the molecular lesions and motility phenotypes of several bop5 alleles in Chlamydomonas reinhardtii. We first characterize a mutation (bop5-2) that disrupts an IC138 protein sub-complex located at the base of the I1 inner arm dynein. We found the bop5-2 deletion also affects the Tubby-1 (TBY1) gene. To characterize TBY1's activity, tagged versions of TBY1 were transformed into bop5-2. TBY1 protein localizes to a unique ring shaped structure found between the two contractile vacuoles and within the nucleo-flagellar apparatus. The bop5-3, bop5-4 and bop5-5 strains, like other I1 mutants, swim forwards with reduced swimming velocities and display an impaired reversal response during photoshock. However, unlike mutants lacking the entire I1 complex, bop5 strains exhibit normal phototaxis. Analysis of the bop5-3 flagellar waveform reveals that loss of the IC138 sub-complex reduces shear amplitude, sliding velocities, and the speed of bend propagation. The results indicate that the IC138 sub-complex is necessary to generate an efficient waveform for optimal forwards and backwards motility, but it is not essential for phototaxis. Assembly and maintenance of eukaryotic cilia and flagella requires the conserved, bidirectional movement of protein complexes along the length of the axoneme known as intraflagellar transport (IFT). We characterize the function of various components of the IFT complex responsible for the retrograde transport of particles towards the cell body. We quantify the defects in retrograde IFT and flagella assembly observed in a series of mutants of the retrograde complex subunit LIC. We also analyze the expression and distribution of retrograde IFT components in a family of flagellar assembly mutants known as fla, and attempt to correlate these patterns with defects in IFT parameters and other behavioral phenotypes. We provide new evidence that defects in IFT motors can alter photoshock and phototaxis behaviors.

Subjects/Keywords: Chlamydomonas; Dynein; Flagella; Intraflagellar transport; Motility

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APA (6^th Edition):

VanderWaal Mills, K. E. (2011). Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/165777

Chicago Manual of Style (16^th Edition):

VanderWaal Mills, Kristyn E. “Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii.” 2011. Doctoral Dissertation, University of Minnesota. Accessed March 03, 2021. http://hdl.handle.net/11299/165777.

MLA Handbook (7^th Edition):

VanderWaal Mills, Kristyn E. “Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii.” 2011. Web. 03 Mar 2021.

Vancouver:

VanderWaal Mills KE. Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. University of Minnesota; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/11299/165777.

Council of Science Editors:

VanderWaal Mills KE. Flagellar proteins regulating motility, assembly and photobehavior in Chlamydomonas reinhardtii. [Doctoral Dissertation]. University of Minnesota; 2011. Available from: http://hdl.handle.net/11299/165777

20. Wilson, Kate. Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure.

Degree: PhD, Mechanical Engineering & Materials Science, 2015, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/eng_etds/102

► Cilia and flagella are subcellular organelles used to generate fluid flow or propel the cell. These long cylindrical structures are composed of cytoskeletal elements… (more)

Subjects/Keywords: Axoneme; Chlamydomonas; Cilia; Flagella; Modeling; Viscosity; Engineering

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APA (6^th Edition):

Wilson, K. (2015). Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/102

Chicago Manual of Style (16^th Edition):

Wilson, Kate. “Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure.” 2015. Doctoral Dissertation, Washington University in St. Louis. Accessed March 03, 2021. https://openscholarship.wustl.edu/eng_etds/102.

MLA Handbook (7^th Edition):

Wilson, Kate. “Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure.” 2015. Web. 03 Mar 2021.

Vancouver:

Wilson K. Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2015. [cited 2021 Mar 03]. Available from: https://openscholarship.wustl.edu/eng_etds/102.

Council of Science Editors:

Wilson K. Probing Mechanical Forces in Flagella by Manipulation of Media Viscosity and Axonemal Structure. [Doctoral Dissertation]. Washington University in St. Louis; 2015. Available from: https://openscholarship.wustl.edu/eng_etds/102

University of Arizona

21. Boyd, Joseph Samuel. Eyespot Assembly and Positioning in Chlamydomonas reinhardtii .

Degree: 2011, University of Arizona

URL: http://hdl.handle.net/10150/145298

► The eyespot of the biflagellate unicellular green alga Chlamydomonas reinhardtii is a complex organelle that facilitates directional responses of the cell to environmental light stimuli.… (more)

▼ The eyespot of the biflagellate unicellular green alga Chlamydomonas reinhardtii is a complex organelle that facilitates directional responses of the cell to environmental light stimuli. The eyespot, which assembles de novo after every cell division and retains a distinctive association with the microtubule cytoskeleton, comprises an elliptical patch of rhodopsin photoreceptors in the plasma membrane and stacks of carotenoid-rich pigment granule arrays in the chloroplast and serves as a model for understanding how organelles are formed and placed asymmetrically in the cell. This study describes the roles of several factors in the assembly and positioning of the eyespot. Two loci, EYE2 and EYE3, define factors involved in the formation and organization of the eyespot pigment granule arrays. Whereas EYE3, a serine/threonine kinase of the ABC1 family, localizes to pigment granules, EYE2 localization corresponds to an area of the chloroplast envelope in the eyespot. These proteins play interdependent roles: EYE2 and the ChR1 photoreceptor co-position in the absence of pigment granules, and the pigment granules are required to maintain the shape and integrity of the EYE2/ChR1 patch. The miniature-eyespot locus MIN2 affects eyespot size and likely regulates the amount of material available for eyespot assembly. The MLT2 locus regulates eyespot size, number, and asymmetry. A novel locus, PEY1, modulates the position of the eyespot on the anterior-posterior axis by affecting microtubule rootlet length. A working model is developed wherein rootlet microtubule-directed photoreceptor localization establishes connections in the chloroplast envelope with EYE2, which directs the site for pigment granule array assembly, and MLT2 is proposed to negatively regulate the levels of eyespot proteins. Advisors/Committee Members: Dieckmann, Carol L (advisor), Weinert, Ted (committeemember), Tax, Frans E. (committeemember), Nagy, Lisa (committeemember), Fares, Hanna (committeemember).

Subjects/Keywords: Chlamydomonas; eyespot; microtubule rootlet; organelle biogenesis

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APA (6^th Edition):

Boyd, J. S. (2011). Eyespot Assembly and Positioning in Chlamydomonas reinhardtii . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/145298

Chicago Manual of Style (16^th Edition):

Boyd, Joseph Samuel. “Eyespot Assembly and Positioning in Chlamydomonas reinhardtii .” 2011. Doctoral Dissertation, University of Arizona. Accessed March 03, 2021. http://hdl.handle.net/10150/145298.

MLA Handbook (7^th Edition):

Boyd, Joseph Samuel. “Eyespot Assembly and Positioning in Chlamydomonas reinhardtii .” 2011. Web. 03 Mar 2021.

Vancouver:

Boyd JS. Eyespot Assembly and Positioning in Chlamydomonas reinhardtii . [Internet] [Doctoral dissertation]. University of Arizona; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10150/145298.

Council of Science Editors:

Boyd JS. Eyespot Assembly and Positioning in Chlamydomonas reinhardtii . [Doctoral Dissertation]. University of Arizona; 2011. Available from: http://hdl.handle.net/10150/145298

University of Arizona

22. Thompson, Mark David. Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii .

Degree: 2016, University of Arizona

URL: http://hdl.handle.net/10150/620817

► The eyespot of the single-celled alga Chlamydomonas aids the cell in detecting the direction of light in the environment. The complex assembly and asymmetric placement… (more)

Subjects/Keywords: ChR1; Eyespot; Molecular & Cellular Biology; Chlamydomonas

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APA (6^th Edition):

Thompson, M. D. (2016). Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/620817

Chicago Manual of Style (16^th Edition):

Thompson, Mark David. “Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii .” 2016. Doctoral Dissertation, University of Arizona. Accessed March 03, 2021. http://hdl.handle.net/10150/620817.

MLA Handbook (7^th Edition):

Thompson, Mark David. “Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii .” 2016. Web. 03 Mar 2021.

Vancouver:

Thompson MD. Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii . [Internet] [Doctoral dissertation]. University of Arizona; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10150/620817.

Council of Science Editors:

Thompson MD. Channelrhodopsin-1: Cellular Localization and Role in Eyespot Assembly and Placement in Chlamydomonas reinhardtii . [Doctoral Dissertation]. University of Arizona; 2016. Available from: http://hdl.handle.net/10150/620817

23. Lachapelle, Josianne Lyse. Role of chance and history during evolution in Chlamydomonas reinhardtii.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/15949

► The extent to which evolution is repeatable has important implications. If evolution is highly repeatable, the trajectories and outcomes of evolution in different lineages will… (more)

▼ The extent to which evolution is repeatable has important implications. If evolution is highly repeatable, the trajectories and outcomes of evolution in different lineages will always be the same. On the other hand, if evolution is not repeatable, then trajectories and outcomes will be diverse. Thus, the repeatability of evolution affects our understanding of the nature of biodiversity and can inform the extent to which evolutionary theory can be used to make predictions. The repeatability of evolution depends on the relative contribution of selection, chance, and history. To determine what factors affect the importance of chance and history during evolution, I propagated replicated populations of the unicellular green alga Chlamydomonas reinhardtii in controlled environments. I measured the change in fitness after a few hundred generations and determined how much variation had arisen among replicate populations and among populations with different histories. I applied a similar approach to study the importance of history in extinctions, and measured rates of extinction in populations with different histories. I found that evolution is much less repeatable in small than in large populations because history is more constraining and selection less efficient in small than in large populations. There is also a significant effect of sex and recombination on the repeatability of evolution at the fitness level, but this effect is highly dependent on the environment of selection. Sex can increase the importance of chance or history in some environments, but lower their importance in others, thereby leading to convergence or divergence depending on the environment. Thirdly, I found that the importance of history during evolution does not appear to come from the accumulation of past evolutionary selection pressures, but rather comes from only the most recent selection pressure as it determines genetic correlations for growth between different environments and the amount of genetic variance. Finally, I found that extinction risks are extremely high during continuous environmental deterioration, although a history of sexual reproduction and phenotypic plasticity play an important role in adaptation. By focusing not solely on the effect of treatments on mean trait values, but also on the variance that arises in our evolution experiments, we can gain a better understanding of the contribution that chance and history make to evolution. The repeatability of evolution can therefore inform us about the adaptive vs. stochastic nature of the diversity we see today, and about the specificity or generality of evolutionary outcomes.

Subjects/Keywords: 579.5; Chlamydomonas reinhardtii; evolution; deterministic; adaption

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APA (6^th Edition):

Lachapelle, J. L. (2016). Role of chance and history during evolution in Chlamydomonas reinhardtii. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/15949

Chicago Manual of Style (16^th Edition):

Lachapelle, Josianne Lyse. “Role of chance and history during evolution in Chlamydomonas reinhardtii.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed March 03, 2021. http://hdl.handle.net/1842/15949.

MLA Handbook (7^th Edition):

Lachapelle, Josianne Lyse. “Role of chance and history during evolution in Chlamydomonas reinhardtii.” 2016. Web. 03 Mar 2021.

Vancouver:

Lachapelle JL. Role of chance and history during evolution in Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1842/15949.

Council of Science Editors:

Lachapelle JL. Role of chance and history during evolution in Chlamydomonas reinhardtii. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/15949

Louisiana State University

24. Mukherjee, Bratati. Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii.

Degree: PhD, 2013, Louisiana State University

URL: etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722

► Photosynthetic microalgae optimize the utilization of inorganic carbon by active uptake and concentration of inorganic carbon (Ci) around the carbon-fixing enzyme Rubisco. This process, mostly… (more)

▼ Photosynthetic microalgae optimize the utilization of inorganic carbon by active uptake and concentration of inorganic carbon (Ci) around the carbon-fixing enzyme Rubisco. This process, mostly induced under limiting carbon conditions is called the Carbon Concentrating Mechanism or CCM. The photosynthetic green alga Chlamydomonas reinhardtii serves as an excellent model organism for the study of eukaryotic CCMs. However, unlike the prokaryotic cyanobacterial CCM, which has a well-characterized Ci uptake system, the Ci uptake and transport system of the C. reinhardtii CCM is not well understood. Since CO2 is a small neutral molecule, it is believed to be able to passively diffuse into the cell with or without any assistance from membrane bound transport proteins. However, the charged HCO3- ion needs transporters to facilitate its uptake across each membrane barrier. The hydrophobic barriers posed by the plasma membrane, chloroplast envelope and thylakoid membranes in the path of the charged HCO3- ion’s ultimate destination to the thylakoid lumen has led to the proposed existence of one or more transport proteins at each of these membrane locations. The roles of the C. reinhardtii NAR gene family, showing sequence homology to the Formate/Nitrite transporter family, were investigated for any changes with respect to the induction of the CCM. NAR1.2 was found to be the most interesting NAR protein with respect to the CCM. NAR1.2 was localized to the chloroplast envelope and is believed to be part of the chloroplastic Ci uptake system. The previously identified putative Ci transporter, LCI1 was localized to the plasma membrane. The expression of this protein in a LCI1 deficient background showed a significant increase in Ci uptake and Ci affinity of cells even in the absence of a functional CCM. However, the RNAi mediated knockdown of the protein failed to show any growth deficiencies or changes in photosynthetic rates at different pH levels. LCI1 is a transporter that is part of the plasma membrane Ci uptake system. In the quest for mutations in either previously identified or novel Ci transporters, an insertional mutagenesis project was also undertaken. The sequenced wild-type strain, CC-503, was found to be a natural mutant for the periplasmic carbonic anhydrase, CAH1.

Subjects/Keywords: insertional mutagenesis; Chlamydomonas reinhardtii; inorganic carbon; CCM

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APA (6^th Edition):

Mukherjee, B. (2013). Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722

Chicago Manual of Style (16^th Edition):

Mukherjee, Bratati. “Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii.” 2013. Doctoral Dissertation, Louisiana State University. Accessed March 03, 2021. etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722.

MLA Handbook (7^th Edition):

Mukherjee, Bratati. “Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii.” 2013. Web. 03 Mar 2021.

Vancouver:

Mukherjee B. Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Louisiana State University; 2013. [cited 2021 Mar 03]. Available from: etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722.

Council of Science Editors:

Mukherjee B. Investigation of the role of putative inorganic carbon transporters in the carbon dioxide concentrating mechanisms of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Louisiana State University; 2013. Available from: etd-07062013-211518 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2722

Louisiana State University

25. Mukherjee, Ananya. Identification and Characterization of Novel Transporters Involved in the CO2 Concentrating Mechanism of Chlamydomonas reinhardtii.

Degree: PhD, Biochemistry, 2019, Louisiana State University

URL: https://digitalcommons.lsu.edu/gradschool_dissertations/4886

► The green alga Chlamydomonas reinhardtii possesses a CO₂ concentrating mechanism (CCM) which helps in successful acclimation to low CO₂ conditions. One of the main… (more)

▼ The green alga Chlamydomonas reinhardtii possesses a CO₂ concentrating mechanism (CCM) which helps in successful acclimation to low CO₂ conditions. One of the main aspects of the CCM is bringing in inorganic carbon (Ci) into the cell as bicarbonate using Ci transporters. Current models of the CCM postulate that a series of ion transporters bring HCO₃^- from outside the cell to the thylakoid lumen where the carbonic anhydrase, CAH3, dehydrates accumulated HCO₃^- to CO₂, raising the CO₂ concentration for Rubisco. Previously, HCO₃^- transporters have been identified at both the plasma membrane (HLA3 and LCI1) and the chloroplast envelope (LCIA/NAR1.2), but the transporter thought to be on the thylakoid membrane has not been identified. The goal of this thesis has been to find the putative thylakoid transporter using a bioinformatics approach to identify candidate proteins followed by characterization of the interesting transporters using RNAi. Nine candidate proteins were identified from the Chlamydomonas proteome which had transporter like domains and were upregulated in low CO_2.Three of the candidates are the paralogous genes (BST1, BST2, BST3) belonging to the bestrophin family, which are not only upregulated in low CO₂ conditions but their expression is controlled by CIA5, a transcription factor known to control Ci transporters of the CCM. YFP fusions demonstrate that all three proteins are located on the thylakoid membrane and interactome studies indicate that they might associate with other chloroplast CCM components. A single mutant defective in BST3, still grows normally on low CO₂, indicating that the three bestrophin-like proteins may have redundant functions. Therefore, an RNAi approach was adopted to reduce the expression of all three genes at once. RNAi mutants with reduced expression of BST1-3, were unable to grow at low CO₂ concentrations, exhibited a reduced affinity for inorganic carbon compared to the wild type cells, and exhibited reduced inorganic carbon uptake. We propose that these bestrophin-like proteins are essential components of the CCM and deliver HCO₃^- accumulated in the chloroplast stroma to CAH3 inside the thylakoid lumen Another mutant , A144 was discovered to be a part of a large-scale mutagenesis project to select for mutants that have been transformed with a paromomycin cassette and grow slowly under low CO₂ growth conditions. Using a modified form of the adapter PCR method, the location of the insert in A144 was found to be in a gene that codes for a protein with homology of fungal translational elongation factor (eEF3). This mutant cannot adapt to light after prolonged exposure to darkness and has a significantly lower rate of respiration than wild type. Thus we propose that EF3 putatively aids in the synthesis of stress proteins for dark adaptation.

Subjects/Keywords: Chlamydomonas; CO2 concentrating mechanism; bestrophin; carbonic anhydrase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mukherjee, A. (2019). Identification and Characterization of Novel Transporters Involved in the CO2 Concentrating Mechanism of Chlamydomonas reinhardtii. (Doctoral Dissertation). Louisiana State University. Retrieved from https://digitalcommons.lsu.edu/gradschool_dissertations/4886

Chicago Manual of Style (16^th Edition):

Mukherjee, Ananya. “Identification and Characterization of Novel Transporters Involved in the CO2 Concentrating Mechanism of Chlamydomonas reinhardtii.” 2019. Doctoral Dissertation, Louisiana State University. Accessed March 03, 2021. https://digitalcommons.lsu.edu/gradschool_dissertations/4886.

MLA Handbook (7^th Edition):

Mukherjee, Ananya. “Identification and Characterization of Novel Transporters Involved in the CO2 Concentrating Mechanism of Chlamydomonas reinhardtii.” 2019. Web. 03 Mar 2021.

Vancouver:

Mukherjee A. Identification and Characterization of Novel Transporters Involved in the CO2 Concentrating Mechanism of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Louisiana State University; 2019. [cited 2021 Mar 03]. Available from: https://digitalcommons.lsu.edu/gradschool_dissertations/4886.

Council of Science Editors:

Mukherjee A. Identification and Characterization of Novel Transporters Involved in the CO2 Concentrating Mechanism of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Louisiana State University; 2019. Available from: https://digitalcommons.lsu.edu/gradschool_dissertations/4886

Louisiana State University

26. Tural, Baran. Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii.

Degree: MS, 2005, Louisiana State University

URL: etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179

► The regulation of the photorespiratory pathway of Chlamydomonas reinhardtii during a shift from high to low CO2 conditions was investigated. To this end, a set… (more)

Subjects/Keywords: Chlamydomonas reinhardtii; photorespiration

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APA (6^th Edition):

Tural, B. (2005). Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii. (Masters Thesis). Louisiana State University. Retrieved from etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179

Chicago Manual of Style (16^th Edition):

Tural, Baran. “Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii.” 2005. Masters Thesis, Louisiana State University. Accessed March 03, 2021. etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179.

MLA Handbook (7^th Edition):

Tural, Baran. “Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii.” 2005. Web. 03 Mar 2021.

Vancouver:

Tural B. Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii. [Internet] [Masters thesis]. Louisiana State University; 2005. [cited 2021 Mar 03]. Available from: etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179.

Council of Science Editors:

Tural B. Regulation of the expression of photorespiratory genes of Chlamydomonas reinhardtii. [Masters Thesis]. Louisiana State University; 2005. Available from: etd-04082005-141028 ; https://digitalcommons.lsu.edu/gradschool_theses/1179

Louisiana State University

27. Roberts, Benjamin Seth. Development of Microfluidic Devices to Study Algal Chemotaxis and Long-Term Growth Dynamics.

Degree: MSChE, Chemical Engineering, 2016, Louisiana State University

URL: etd-11112016-101845 ; https://digitalcommons.lsu.edu/gradschool_theses/4496

► Harmful algal blooms pose a threat to human health and the environment. Many complex factors influence their formation and development, and much is still unknown.… (more)

Subjects/Keywords: chlamydomonas reinhardtii; chemotaxis; allelopathy; algae; microfluidics

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APA (6^th Edition):

Roberts, B. S. (2016). Development of Microfluidic Devices to Study Algal Chemotaxis and Long-Term Growth Dynamics. (Masters Thesis). Louisiana State University. Retrieved from etd-11112016-101845 ; https://digitalcommons.lsu.edu/gradschool_theses/4496

Chicago Manual of Style (16^th Edition):

Roberts, Benjamin Seth. “Development of Microfluidic Devices to Study Algal Chemotaxis and Long-Term Growth Dynamics.” 2016. Masters Thesis, Louisiana State University. Accessed March 03, 2021. etd-11112016-101845 ; https://digitalcommons.lsu.edu/gradschool_theses/4496.

MLA Handbook (7^th Edition):

Roberts, Benjamin Seth. “Development of Microfluidic Devices to Study Algal Chemotaxis and Long-Term Growth Dynamics.” 2016. Web. 03 Mar 2021.

Vancouver:

Roberts BS. Development of Microfluidic Devices to Study Algal Chemotaxis and Long-Term Growth Dynamics. [Internet] [Masters thesis]. Louisiana State University; 2016. [cited 2021 Mar 03]. Available from: etd-11112016-101845 ; https://digitalcommons.lsu.edu/gradschool_theses/4496.

Council of Science Editors:

Roberts BS. Development of Microfluidic Devices to Study Algal Chemotaxis and Long-Term Growth Dynamics. [Masters Thesis]. Louisiana State University; 2016. Available from: etd-11112016-101845 ; https://digitalcommons.lsu.edu/gradschool_theses/4496

28. Wills, Brandon M. Optimizing Tools for the Investigation of Flagella Protein Trafficking in Chlamydomonas Reinhardtii.

Degree: 2019, Marquette University

URL: https://epublications.marquette.edu/theses_open/621

► Cilia and flagella are synonymous organelles that protrude above the cell surface to propel fluid and monitor signals in the environment. These functions are central… (more)

Subjects/Keywords: Chlamydomonas; Cilia; Flagella; IFT; RSP3; Trafficking; Biology

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APA (6^th Edition):

Wills, B. M. (2019). Optimizing Tools for the Investigation of Flagella Protein Trafficking in Chlamydomonas Reinhardtii. (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/theses_open/621

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wills, Brandon M. “Optimizing Tools for the Investigation of Flagella Protein Trafficking in Chlamydomonas Reinhardtii.” 2019. Thesis, Marquette University. Accessed March 03, 2021. https://epublications.marquette.edu/theses_open/621.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wills, Brandon M. “Optimizing Tools for the Investigation of Flagella Protein Trafficking in Chlamydomonas Reinhardtii.” 2019. Web. 03 Mar 2021.

Vancouver:

Wills BM. Optimizing Tools for the Investigation of Flagella Protein Trafficking in Chlamydomonas Reinhardtii. [Internet] [Thesis]. Marquette University; 2019. [cited 2021 Mar 03]. Available from: https://epublications.marquette.edu/theses_open/621.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wills BM. Optimizing Tools for the Investigation of Flagella Protein Trafficking in Chlamydomonas Reinhardtii. [Thesis]. Marquette University; 2019. Available from: https://epublications.marquette.edu/theses_open/621

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

29. Teplitski, Maxim Igorevich. Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing.

Degree: PhD, Graduate School, 2002, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062

Subjects/Keywords: Biology; Sinorhizobium; Chlamydomonas

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APA (6^th Edition):

Teplitski, M. I. (2002). Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062

Chicago Manual of Style (16^th Edition):

Teplitski, Maxim Igorevich. “Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing.” 2002. Doctoral Dissertation, The Ohio State University. Accessed March 03, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062.

MLA Handbook (7^th Edition):

Teplitski, Maxim Igorevich. “Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing.” 2002. Web. 03 Mar 2021.

Vancouver:

Teplitski MI. Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing. [Internet] [Doctoral dissertation]. The Ohio State University; 2002. [cited 2021 Mar 03]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062.

Council of Science Editors:

Teplitski MI. Quorum sensing in Sinorhizobium meliloti and effect of plant signals on bacterial quorum sensing. [Doctoral Dissertation]. The Ohio State University; 2002. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463803600062

30. Nguyen, Thi hoa mai. Etude du métabolisme lipidique chez Clamydomonas reinhardtii : Approches de protéomique et de génétique : Study of lipid metabolism in Chlamydomonas reinhardtii by proteomic and genetic approaches.

Degree: Docteur es, Biologie végétale, 2013, Aix Marseille Université

URL: http://www.theses.fr/2013AIXM4006

►

La capacité des microalgues à accumuler des quantités importantes de lipides de réserve font de ces organismes de bons candidats pour envisager une production durable… (more)

▼

La capacité des microalgues à accumuler des quantités importantes de lipides de réserve font de ces organismes de bons candidats pour envisager une production durable de biocarburants (biodiesel). Cependant, des verrous d’ordre technologique et biologique persistent avant d’atteindre une production économiquement viable. Dans le but de mieux comprendre les mécanismes et biosynthèse et d’accumulation des lipides chez les microalgues et de proposer des voies d’amélioration biotechnologiques, nous avons développé deux approches expérimentales complémentaires en utilisant la microalgue Chlamydomonas reinhardtii comme modèle. La première a été de caractériser par des techniques de protéomique et de lipidomique la composition des gouttelettes lipidiques s’accumulant en réponse à une carence en azote. Les données de protéomique nous ont permis de montrer que les gouttelettes lipidiques étaient des structures cellulaires dynamiques impliquées non seulement dans le stockage, mais aussi dans la biosynthèse, la remobilisation et le « trafficking » des lipides. Les protéines identifiées au cours de cette étude nous fournissent des gènes cibles d’intérêt pour mieux comprendre les voies de biosynthèse des triacylglycérols et accroître l’accumulation d’huile. La seconde approche, de génétique formelle, a consisté à rechercher puis à caractériser des mutants isolés à partir d’une banque de mutants d’insertion de C. reinhardtii. Deux mutants d’intérêt, l’un affecté dans la composition en acides gras (crfad7) et l’autre capable d’accumuler des lipides en l’absence de stress (coa1, pour constitutive oil accumulator 1), ont été isolés.

The ability of microalgae to accumulate high amounts of reserve lipids makes these organisms good candidates for the production of sustainable biofuel (biodiesel). However, both technological and biological bottlenecks remain to be overcome before profitable production is reached. With the aim to better understand lipid metabolic pathways in microalgae and further propose new strategies for biotechnological improvement, we have developed two complementary experimental approaches in the model microalga Chlamydomonas reinhardtii. As a first approach, we performed a proteomic and lipidomic characterization of oil bodies isolated from nitrogen-deprived cells. Based on proteomic data, we have concluded that oil bodies are dynamic structures involved not only in the storage, but also in oil biosynthesis, degradation and lipid homeostasis. The proteins identified in this study should provide useful targets for genetic studies aiming at increasing our understanding of triacylglycerol synthesis and further improve intracellular oil accumulation. The second approach, based on the development of a forward genetic screen, aimed at searching and further characterizing mutants isolated from a C. reinhardtii insertion library. Two mutants of interest, one affected in the fatty acid composition (crfad7), the other (coa1, for constitutive oil accumulator 1) able to accumulate reserve lipids in the absence of stress,…

Advisors/Committee Members: Peltier, Gilles (thesis director).

Subjects/Keywords: Acides gras; Lipides; Triacylglycérol; Désaturase; Chlamydomonas; Fatty acid; Desaturase; Triacylglycerols; Oil bodies; Chlamydomonas; 581

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APA (6^th Edition):

Nguyen, T. h. m. (2013). Etude du métabolisme lipidique chez Clamydomonas reinhardtii : Approches de protéomique et de génétique : Study of lipid metabolism in Chlamydomonas reinhardtii by proteomic and genetic approaches. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2013AIXM4006

Chicago Manual of Style (16^th Edition):

Nguyen, Thi hoa mai. “Etude du métabolisme lipidique chez Clamydomonas reinhardtii : Approches de protéomique et de génétique : Study of lipid metabolism in Chlamydomonas reinhardtii by proteomic and genetic approaches.” 2013. Doctoral Dissertation, Aix Marseille Université. Accessed March 03, 2021. http://www.theses.fr/2013AIXM4006.

MLA Handbook (7^th Edition):

Nguyen, Thi hoa mai. “Etude du métabolisme lipidique chez Clamydomonas reinhardtii : Approches de protéomique et de génétique : Study of lipid metabolism in Chlamydomonas reinhardtii by proteomic and genetic approaches.” 2013. Web. 03 Mar 2021.

Vancouver:

Nguyen Thm. Etude du métabolisme lipidique chez Clamydomonas reinhardtii : Approches de protéomique et de génétique : Study of lipid metabolism in Chlamydomonas reinhardtii by proteomic and genetic approaches. [Internet] [Doctoral dissertation]. Aix Marseille Université 2013. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2013AIXM4006.

Council of Science Editors:

Nguyen Thm. Etude du métabolisme lipidique chez Clamydomonas reinhardtii : Approches de protéomique et de génétique : Study of lipid metabolism in Chlamydomonas reinhardtii by proteomic and genetic approaches. [Doctoral Dissertation]. Aix Marseille Université 2013. Available from: http://www.theses.fr/2013AIXM4006

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Open Access Theses and Dissertations