subject:(Cell surface engineering)

Université Catholique de Louvain

1. Philippart, Catherine. Nanopatterned surfaces to control the development of bacteria and mammalian cells.

Degree: 2018, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/195701

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The development of surfaces controlling both bacterial and mammalian cell behaviors is of a great interest for applications in tissue engineering. The challenge is to… (more)

Subjects/Keywords: Nanoimprint lithography; Cell adhesion; Chemical nanopattern; Surface functionalization; Tissue engineering; Peptide; Stem cell; Cell differentiation; Cell morphology; Bacteria

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APA (6^th Edition):

Philippart, C. (2018). Nanopatterned surfaces to control the development of bacteria and mammalian cells. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/195701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Philippart, Catherine. “Nanopatterned surfaces to control the development of bacteria and mammalian cells.” 2018. Thesis, Université Catholique de Louvain. Accessed January 19, 2021. http://hdl.handle.net/2078.1/195701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Philippart, Catherine. “Nanopatterned surfaces to control the development of bacteria and mammalian cells.” 2018. Web. 19 Jan 2021.

Vancouver:

Philippart C. Nanopatterned surfaces to control the development of bacteria and mammalian cells. [Internet] [Thesis]. Université Catholique de Louvain; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2078.1/195701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Philippart C. Nanopatterned surfaces to control the development of bacteria and mammalian cells. [Thesis]. Université Catholique de Louvain; 2018. Available from: http://hdl.handle.net/2078.1/195701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Iowa

2. Ahmed, Kawther Khalid. Assembly and characterization of a cell-particle hybrid system as a potential cancer vaccine.

Degree: MS, Pharmacy, 2013, University of Iowa

URL: https://ir.uiowa.edu/etd/3034

► Cancer vaccines represent a promising treatment modality for a world-wide health problem. Whether as an adjuvant or as a stand-alone therapy, cancer vaccines represent… (more)

▼ Cancer vaccines represent a promising treatment modality for a world-wide health problem. Whether as an adjuvant or as a stand-alone therapy, cancer vaccines represent a tumor-specific and systemic treatment potentially capable of eliminating metastatic lesions without the severe side-effects often associated with chemotherapy. Specifically, whole cell tumor vaccines have shown promise in preclinical and clinical settings and the studies presented here represent the beginnings of an approach to improve the antitumor potency of these vaccines. This project demonstrates as "proof of concept" the feasibility of manufacturing tumor cell-particle hybrids. The coupled use of these two components, whole tumor cells and cargo-carrying biodegradable particles, as one entity in a cancer vaccine system is a new line of research. Stable cell-particle hybrids were assembled using avidin-biotin chemistry where cargo-carrying PLGA particles (500 nm diameter) were coated with streptavidin and allowed to bind to tumor cells that had been indirectly labeled with biotin (using an integrin-specific biotinylated antibody). That successful cell-particle hybrids were assembled was determined by multiple means, including flow cytometry, laser scanning confocal microscopy and scanning electron microscopy. Two murine tumor cell lines (representing melanoma and prostate cancer) were investigated in this study and successfully demonstrated the general applicability of the assembly method. Particles appeared to be localized on the cell surface (rather than endocytosed) as determined by microscopic imaging. The cell-particle hybrid was shown to be stable to irradiation, an important consideration since whole tumor cells need to be treated with ionizing radiation prior to being used as vaccines in order to render them nonproliferative and immunogenic. We also characterized loading and release profiles of CpG, a prospective vaccine adjuvant, into PLGA particles. We conclude that we have developed a method for manufacturing cell-particle hybrids comprising PLGA nanoparticles and irradiated tumor cells. The next step would be to use CpG-loaded particles in the assembled hybrid and test the anti-tumor immune efficiency of this cancer vaccine formulation in either a melanoma or prostate cancer model. Advisors/Committee Members: Salem, Aliasger K. (supervisor).

Subjects/Keywords: avidin-biotin; cancer vaccine; cell-particle hybrid; cell surface engineering; Pharmacy and Pharmaceutical Sciences

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APA (6^th Edition):

Ahmed, K. K. (2013). Assembly and characterization of a cell-particle hybrid system as a potential cancer vaccine. (Masters Thesis). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/3034

Chicago Manual of Style (16^th Edition):

Ahmed, Kawther Khalid. “Assembly and characterization of a cell-particle hybrid system as a potential cancer vaccine.” 2013. Masters Thesis, University of Iowa. Accessed January 19, 2021. https://ir.uiowa.edu/etd/3034.

MLA Handbook (7^th Edition):

Ahmed, Kawther Khalid. “Assembly and characterization of a cell-particle hybrid system as a potential cancer vaccine.” 2013. Web. 19 Jan 2021.

Vancouver:

Ahmed KK. Assembly and characterization of a cell-particle hybrid system as a potential cancer vaccine. [Internet] [Masters thesis]. University of Iowa; 2013. [cited 2021 Jan 19]. Available from: https://ir.uiowa.edu/etd/3034.

Council of Science Editors:

Ahmed KK. Assembly and characterization of a cell-particle hybrid system as a potential cancer vaccine. [Masters Thesis]. University of Iowa; 2013. Available from: https://ir.uiowa.edu/etd/3034

UCLA

3. Zhao, Yitong. Optimizing Biodiesel Production of a Cell-Free System by Feedback System Control Scheme.

Degree: Biomedical Engineering, 2014, UCLA

URL: http://www.escholarship.org/uc/item/2qz609m0

► Due to environmental benefits, rising crude oil prices, and limited resources of fossil oil, there has been renewed focus on vegetable oils as a source… (more)

Subjects/Keywords: Biomedical engineering; Algae; Biofuel; Biotechnology; Cell-free; FSC; Surface Respond Method

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APA (6^th Edition):

Zhao, Y. (2014). Optimizing Biodiesel Production of a Cell-Free System by Feedback System Control Scheme. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/2qz609m0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhao, Yitong. “Optimizing Biodiesel Production of a Cell-Free System by Feedback System Control Scheme.” 2014. Thesis, UCLA. Accessed January 19, 2021. http://www.escholarship.org/uc/item/2qz609m0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhao, Yitong. “Optimizing Biodiesel Production of a Cell-Free System by Feedback System Control Scheme.” 2014. Web. 19 Jan 2021.

Vancouver:

Zhao Y. Optimizing Biodiesel Production of a Cell-Free System by Feedback System Control Scheme. [Internet] [Thesis]. UCLA; 2014. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/2qz609m0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhao Y. Optimizing Biodiesel Production of a Cell-Free System by Feedback System Control Scheme. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/2qz609m0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

4. Tran, Phat L. Nanoscale Feature Composite: An Ensemble Surface for Enhancing Cardiovascular Implant Endothelialization .

Degree: 2011, University of Arizona

URL: http://hdl.handle.net/10150/203530

► The establishment and maintenance of functional endothelial cells (ECs) on an engineered surface is central to tissue engineering. As the field advances, the role of… (more)

▼ The establishment and maintenance of functional endothelial cells (ECs) on an engineered surface is central to tissue engineering. As the field advances, the role of cellular mechanisms, particularly the adhesive interaction between the surface of implantable devices and biological systems, becomes more relevant in both research and clinical practice. Knowledge of these interactions can address many fundamental biological questions and would provide key design parameters for medical implants. It has been shown that EC functionality and adhesivity, crucial for the re-endothelialization process, can be induced by nanotopographical modification. Therefore, the goal of this dissertation research was to develop an ensemble surface composing of nanoscale features for the enhancement of endothelial cell adhesion. Without adhesion, subsequent vital mechanism involved in cell alignment, elongation or spreading, proliferation, migration, and ECM proteins deposition will not occur.Experiments in support of this goal were broken down into three specific aims. The first aim was to characterize and develop a size-dependent self-assembly (SDSA) nanoarray of Octamer transcription factor 4 as a demonstration to the fabrication of nanoscale feature surface. This nanoparticle array platform was a pilot studied for the second aim, which was the development of an ensemble surface of nanoscale features for endothelial cell adhesion. The third aim was to evaluate and assess EC response to the ensemble surface.Hence, we developed an ensemble surface composed of nanoscale features and adhesive elements for EC adhesivity. By using shear stress as a detachment force, we demonstrated greater cell retention by the ensemble surface than uniform controls. Adhesive interactions and cellular migration through integrin expressions, which are critical to tissue development and wound healing process was also observed. Furthermore, cell viability was relatively sustainable, as indicated by the low expression of apoptotic signaling molecules. The findings presented within this dissertation research can be applicable to blood-contact medical implants and possess the potential for future clinical translation. Advisors/Committee Members: Yoon, Jeong-Yeol (advisor), Slepian, Marvin J. (committeemember), Riley, Mark R. (committeemember), Wong, Pak K. (committeemember), Wu, Xiaoyi (committeemember), Yoon, Jeong-Yeol (committeemember).

Subjects/Keywords: ensemble surface; nanoscale textured; Biomedical Engineering; endothelial cell; endothelialization

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APA (6^th Edition):

Tran, P. L. (2011). Nanoscale Feature Composite: An Ensemble Surface for Enhancing Cardiovascular Implant Endothelialization . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/203530

Chicago Manual of Style (16^th Edition):

Tran, Phat L. “Nanoscale Feature Composite: An Ensemble Surface for Enhancing Cardiovascular Implant Endothelialization .” 2011. Doctoral Dissertation, University of Arizona. Accessed January 19, 2021. http://hdl.handle.net/10150/203530.

MLA Handbook (7^th Edition):

Tran, Phat L. “Nanoscale Feature Composite: An Ensemble Surface for Enhancing Cardiovascular Implant Endothelialization .” 2011. Web. 19 Jan 2021.

Vancouver:

Tran PL. Nanoscale Feature Composite: An Ensemble Surface for Enhancing Cardiovascular Implant Endothelialization . [Internet] [Doctoral dissertation]. University of Arizona; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10150/203530.

Council of Science Editors:

Tran PL. Nanoscale Feature Composite: An Ensemble Surface for Enhancing Cardiovascular Implant Endothelialization . [Doctoral Dissertation]. University of Arizona; 2011. Available from: http://hdl.handle.net/10150/203530

University of Rochester

5. Tsai, Hsin-Yi (1981 - ). Thermo-responsive poly(N-isopropylacrylamide) microgel particles for biomedical applications.

Degree: PhD, 2013, University of Rochester

URL: http://hdl.handle.net/1802/27157

► Colloidal PNIPAM hydrogel particles have found potential applications in the biomedical field because of their thermo-responsiveness. In the current work, two novel applications of PNIPAM… (more)

▼ Colloidal PNIPAM hydrogel particles have found potential applications in the biomedical field because of their thermo-responsiveness. In the current work, two novel applications of PNIPAM microgels are demonstrated. PNIPAM microgels were engineered to serve as thermo-responsive protein transfer agents, which can be applied to modify the surface of 2-D photonic crystals to create ultrasensitive biosensors. The particles were functionalized with metal chelating groups to enable the reversible affinity binding of peptides or proteins, and also grafted with polymeric stabilizers to maintain the colloidal stability under physiological conditions. Two designs were demonstrated in the study. The first type of particle was synthesized by incorporating the stabilizers and the functional groups separately via a two-stage dispersion polymerization. Another type of particle was copolymerized with end-functionalized stabilizers that can be readily conjugated to the chelating groups. Both types of particles were thermo-sensitive, colloidally stable, and able to reversibly bind to the model peptides. Nonionic block copolymers were used as surfactants for the dispersion polymerization of PNIPAM microgel particles to replace the less biocompatible ionic surfactants. The surfactants stabilized PNIPAM particles through physical adsorption but not chemical grafting. The effectiveness of the surfactants was evaluated by comparing the size of the resulting particles. Nonionic surfactants were also found to successfully enhance the colloidal stability at the post-polymerization stage. This allows one to use PNIPAM microgels in physiological environment in the form of particle dispersions without altering the particle composition and polymerization process. PNIPAM microgels were also deposited in micropatterns on substrates for the cell sheet engineering application. A simple dip coating method was employed to micropattern flat substrates with PNIPAM particles in a template free manner. PNIPAM particles self-assembled into 2-D micropatterns, where stripe and spacing regions consisted of densely packed particles and sparsely distributed particles, respectively. With this versatile process the dimensions of the PNIPAM micropatterns can be controlled. Preferential adhesion of fibroblast cells was observed on the spacing regions initially, and the confluent cell sheet was obtained later on. The cell sheet successfully detached from the substrates upon cooling as the result of the thermo-responsiveness of PNIPAM.

Subjects/Keywords: Biosensing; Cell sheet engineering; Colloidal stability; Microgel; Poly(N-isopropylacrylamide); Surface engineering

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APA (6^th Edition):

Tsai, H. (. -. ). (2013). Thermo-responsive poly(N-isopropylacrylamide) microgel particles for biomedical applications. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/27157

Chicago Manual of Style (16^th Edition):

Tsai, Hsin-Yi (1981 - ). “Thermo-responsive poly(N-isopropylacrylamide) microgel particles for biomedical applications.” 2013. Doctoral Dissertation, University of Rochester. Accessed January 19, 2021. http://hdl.handle.net/1802/27157.

MLA Handbook (7^th Edition):

Tsai, Hsin-Yi (1981 - ). “Thermo-responsive poly(N-isopropylacrylamide) microgel particles for biomedical applications.” 2013. Web. 19 Jan 2021.

Vancouver:

Tsai H(-). Thermo-responsive poly(N-isopropylacrylamide) microgel particles for biomedical applications. [Internet] [Doctoral dissertation]. University of Rochester; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1802/27157.

Council of Science Editors:

Tsai H(-). Thermo-responsive poly(N-isopropylacrylamide) microgel particles for biomedical applications. [Doctoral Dissertation]. University of Rochester; 2013. Available from: http://hdl.handle.net/1802/27157

York University

6. O'Brien, Paul J. Bio-Orthogonal Manipulation of Cellular Membranes for Biological Applications.

Degree: PhD, Chemistry, 2018, York University

URL: http://hdl.handle.net/10315/34569

► Recent breakthroughs using novel chemistry and nanotechnology methodologies for cellular biology applications have spurred tremendous interest in the development of new technologies to manipulate cell-lines… (more)

▼ Recent breakthroughs using novel chemistry and nanotechnology methodologies for cellular biology applications have spurred tremendous interest in the development of new technologies to manipulate cell-lines or cellular products for biotechnology applications, without complicated or permanent techniques used in academia and industry. Small molecule drugs, proteins, deoxyribonucleic acids (DNA) and ribonucleic acids (RNA) have been employed to manipulate and probe cellular behaviour with the intention of elucidating healthy and diseased molecular pathways for understanding human health. This research focused on the application of liposomal delivery of bioorthogonal chemoselective chemistry to cellular membranes to control biological functions. These smart membranes were used to investigate cellular adhesion, cell spheroid aggregation kinetics and incorporation of ligands. In Chapters 2 and 3, NMR spectroscopy, oxime reaction kinetics and oxime cell surface engineered cell adhesion was correlated and probed under microfluidic conditions, while the robustness of adhesion and flexibility of cluster and tissue formation was established using live cell technique microfluidics. Using this as a base strategy, we developed a general method for the microfluidic manipulation and CSE of cells for dual labelling and flexible delivery of nucleic acids and small molecule ligands (Paul OBrien et al. 2017, in preparation). In Chapter 4, the bioorthogonal liposomal strategy originally utilized for cell adhesion and cell membrane ligand integration was modified and extended to transfect mammalian cell lines with nucleic acids, limiting the use of cationic charge as a mild and general method to tag and target cells in monocultures and co-cultures. The method was characterized using microscopy, protein production quantification and targeting in complex co-cultures for selective internalization. Finally, in Chapter 5 a new chemical moiety termed dialdehyde was designed and synthesized for the easy and mild conjugation of primary amine containing molecules. This conjugation strategy was used to deliver small molecule ligands and macromolecules to bacterial and mammalian cell membranes using a generalized liposomal formation method. The dialdehyde was characterized and modified to contain two dialdehyde moieties with a Polyethylene Glycol (PEG) linker for crosslinking proteins, inorganic surface functionalization, organic bead assembly and cell aggregation for tissue formation. Advisors/Committee Members: Yousaf, Muhammad (advisor).

Subjects/Keywords: Biology; Bio-orthogonal chemistry; Chemistry; Biology; Tissue engineering; Oxime; Liposome; Cell surface engineering

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APA (6^th Edition):

O'Brien, P. J. (2018). Bio-Orthogonal Manipulation of Cellular Membranes for Biological Applications. (Doctoral Dissertation). York University. Retrieved from http://hdl.handle.net/10315/34569

Chicago Manual of Style (16^th Edition):

O'Brien, Paul J. “Bio-Orthogonal Manipulation of Cellular Membranes for Biological Applications.” 2018. Doctoral Dissertation, York University. Accessed January 19, 2021. http://hdl.handle.net/10315/34569.

MLA Handbook (7^th Edition):

O'Brien, Paul J. “Bio-Orthogonal Manipulation of Cellular Membranes for Biological Applications.” 2018. Web. 19 Jan 2021.

Vancouver:

O'Brien PJ. Bio-Orthogonal Manipulation of Cellular Membranes for Biological Applications. [Internet] [Doctoral dissertation]. York University; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10315/34569.

Council of Science Editors:

O'Brien PJ. Bio-Orthogonal Manipulation of Cellular Membranes for Biological Applications. [Doctoral Dissertation]. York University; 2018. Available from: http://hdl.handle.net/10315/34569

University of Washington

7. Jiao, Alex. Engineering of Functional, Striated Muscle Tissues with Controllable 3D Architectures Using a Novel, Thermoresponsive, Nanofabricated Substratum.

Degree: PhD, 2017, University of Washington

URL: http://hdl.handle.net/1773/38580

► Most tissues in the human body demonstrate multiscale organization, from extracellular matrix (ECM) structure, to cell morphologies, to overall tissue architecture. Further, in the cases… (more)

▼ Most tissues in the human body demonstrate multiscale organization, from extracellular matrix (ECM) structure, to cell morphologies, to overall tissue architecture. Further, in the cases of cardiac and skeletal muscle, tissue structure is critical to appropriate tissue function. However, current tissue engineering methods lack the ability to properly recreate scaffold-free, cell dense tissues with physiological structures. A platform which could engineer 3D tissues with controllable architectures could thus enable the study of more complex biological phenomenon, such as the effect of tissue structure on skeletal muscle development and engineered cardiac tissue function. For this reason, we developed a simple, yet versatile platform combining a thermoresponsive nanofabricated substratum (TNFS) incorporating nanotopographical cues and a gel casting method for the fabrication of scaffold-free 3D tissues with controllable architectures. The developed TNFS could be engineered with a variety of nanotopographies and thus cell monolayer structures which can be spontaneously detached via a change in culture temperature. The detached, nanoengineered cell sheets can then be stacked using our gel casting method to engineer specifically structured, 3D tissues. To this end, we first used the developed TNFS to engineer organized myoblast tissues with specific tissue architectures to demonstrate proof of concept engineering of 3D tissues with layer-by-layer architectural control. We found that using the gel casting method and TNFS, individual aligned myoblast sheets can be stacked into trilayer tissues and maintain individual layer alignment and stacked layer angles without reorganization between the individual sheets, whereas unpatterned controls demonstrated reorganization and layer mixing. We then utilized our developed platform to analyze the effects of engineered myoblast tissue structures on myoblast fusion and subsequent myotube morphology and alignment. We found that parallel-aligned myoblast bilayers could differentiate into aligned myotube sheets in a single layer, however orthogonally-oriented myoblast bilayers lost structural organization during differentiation. Additionally, transferred ECM and tissue structure from the TNFS could provide sufficient alignment cues to allow for the formation of aligned muscle tissue in a 3D microenvironment similar to that of the myofiber niche. Finally, we utilized our developed platform to engineer multilayered human cardiac tissues. We first found that by incorporating a vascular cell population capable of producing ECM, aligned human induced Pluripotent Stem (iPS) cell-derived cardiac sheets could be detached and stacked together. We then engineered 4-layer, aligned and helical cardiac tissues as microscale models of physiologically-structured myocardium. Aligned and helical 3D tissues demonstrated different contractile profiles, such as linear and spiraling, and also demonstrated improved contractile function over unpatterned controls, with aligned 3D cardiac tissues demonstrating… Advisors/Committee Members: Kim, Deok-Ho (advisor).

Subjects/Keywords: Biomaterials; Cardiovascular physiology; Nanotopography; Stem cell biology; Surface modification; Tissue engineering; Biomedical engineering; Bioengineering

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APA (6^th Edition):

Jiao, A. (2017). Engineering of Functional, Striated Muscle Tissues with Controllable 3D Architectures Using a Novel, Thermoresponsive, Nanofabricated Substratum. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/38580

Chicago Manual of Style (16^th Edition):

Jiao, Alex. “Engineering of Functional, Striated Muscle Tissues with Controllable 3D Architectures Using a Novel, Thermoresponsive, Nanofabricated Substratum.” 2017. Doctoral Dissertation, University of Washington. Accessed January 19, 2021. http://hdl.handle.net/1773/38580.

MLA Handbook (7^th Edition):

Jiao, Alex. “Engineering of Functional, Striated Muscle Tissues with Controllable 3D Architectures Using a Novel, Thermoresponsive, Nanofabricated Substratum.” 2017. Web. 19 Jan 2021.

Vancouver:

Jiao A. Engineering of Functional, Striated Muscle Tissues with Controllable 3D Architectures Using a Novel, Thermoresponsive, Nanofabricated Substratum. [Internet] [Doctoral dissertation]. University of Washington; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1773/38580.

Council of Science Editors:

Jiao A. Engineering of Functional, Striated Muscle Tissues with Controllable 3D Architectures Using a Novel, Thermoresponsive, Nanofabricated Substratum. [Doctoral Dissertation]. University of Washington; 2017. Available from: http://hdl.handle.net/1773/38580

University of Tennessee – Knoxville

8. Liu, Xifeng. Phenotypic Modulation of Smooth Muscle Cells on Biodegradable Elastomeric Substrates.

Degree: 2014, University of Tennessee – Knoxville

URL: https://trace.tennessee.edu/utk_graddiss/2899

► Cardiovascular disease is the number one killer in the U.S. Cardiovascular tissue engineering holds enormous potential by providing synthetic materials as vessel replacements. This dissertation… (more)

▼ Cardiovascular disease is the number one killer in the U.S. Cardiovascular tissue engineering holds enormous potential by providing synthetic materials as vessel replacements. This dissertation focused on the development of novel biodegradable and photo-crosslinkable polymers with controlled surface chemistry, stiffness, and topographical features in regulating smooth muscle cell (SMC) adhesion, proliferation and phenotypic conversion for cardiovascular tissue engineering applications. Chapter II presents a facile synthesis route to obtain a series of photocrosslinkable poly(epsilon-caprolactone) triacrylates (PCLTA) with varied mechanical properties and further demonstrated tunable cell responses using these polymer system. Chapter III demonstrates a model polymer network from PCLTA that can gradually stiffen in 24 h through impeded crystallization at body temperature (37 ºC) and distinct SMC attachment, proliferation and spreading are found. Chapter IV presents the fabrication of a series of PCLTA networks with defined gradients in stiffness for regulation of SMCs behaviors. Chapter V fabricates cylindrical pillars with three different heights of 3.4, 7.4, and 15.1 micrometers by photo-crosslinking PCLTA in silicon molds with predesigned micropatterns. Chapter VI prepared photo-crosslinked PCLTA nanowire arrays with diameters of 20, 100 and 200 nanometers using inorganic nanoporous aluminum oxide (AAO) templates. Chapter VII reports a series of novel poly(L-lactic acid) triacrylates (PLLATAs) networks with same chemical composition but different crystallinity and surface roughness achieved by increasing the annealing time from 0 to 5, 7, 10, and 20 h at 70 ºC. Chapter VIII presents a method for tuning surface chemistry by grafting hydrophilic photocrosslinkable mPEGA chains into the hydrophobic PCLTA at various compositions and reports the smooth muscle cell responses. Chapter IX incorporates poly(L-lysine) (PLL) dangling chains into PCLTA networks at different PLL compositions of 0.5%, 1.0%, 1.5%, and 3%. The surface morphology, hydrophilicity and serum protein adsorption of all these polymer networks were characterized. Primary rat SMCs were cultured on these polymer networks and their attachment, spreading, proliferation, focal adhesions, expression of four contractile gene markers (SM-MHC, smoothlin, transgelin, and calponin) and contractile proteins were characterized systematically. Chapter X makes a summary of these separate investigations and draws general conclusions from the results obtained in these studies.

Subjects/Keywords: Biodegradable Polymer; Biomaterials; Nanofabrication; Cell-Material Interface; Tissue Engineering; Surface Chemistry; Materials Science and Engineering

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APA (6^th Edition):

Liu, X. (2014). Phenotypic Modulation of Smooth Muscle Cells on Biodegradable Elastomeric Substrates. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_graddiss/2899

Chicago Manual of Style (16^th Edition):

Liu, Xifeng. “Phenotypic Modulation of Smooth Muscle Cells on Biodegradable Elastomeric Substrates.” 2014. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed January 19, 2021. https://trace.tennessee.edu/utk_graddiss/2899.

MLA Handbook (7^th Edition):

Liu, Xifeng. “Phenotypic Modulation of Smooth Muscle Cells on Biodegradable Elastomeric Substrates.” 2014. Web. 19 Jan 2021.

Vancouver:

Liu X. Phenotypic Modulation of Smooth Muscle Cells on Biodegradable Elastomeric Substrates. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2014. [cited 2021 Jan 19]. Available from: https://trace.tennessee.edu/utk_graddiss/2899.

Council of Science Editors:

Liu X. Phenotypic Modulation of Smooth Muscle Cells on Biodegradable Elastomeric Substrates. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2014. Available from: https://trace.tennessee.edu/utk_graddiss/2899

University of Waterloo

9. Al Shakhshir, Saher. Surface Wettability Impact on Water Management in PEM Fuel Cell.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/7095

► Excessive water formation inside the polymer electrolyte membrane (PEM) fuel cell’s structures leads to the flooding of the cathode gas diffusion layer (GDL) and cathode… (more)

▼ Excessive water formation inside the polymer electrolyte membrane (PEM) fuel cell’s structures leads to the flooding of the cathode gas diffusion layer (GDL) and cathode gas flow channels. This results in a negative impact on water management and the overall cell performance. Liquid water generated in the cathode catalyst layer and the water moved from anode to cathode side due to electro-osmotic drag transport through the GDL to reach the gas flow field channels, where it is removed by air cathode gas stream. Due to high and uniform capillary force distribution effect of the pores through the GDL plane and surface tension between the water droplets and gas flow field channels surfaces, liquid water tends to block/fill the pores of the GDL and stick to the surface of the GDL and gas flow channels. Therefore, it is difficult to remove the trapped water in GDL structure which can lead to flood of the PEM fuel cell. The GDL surfaces are commonly treated uniformly with a hydrophobic material in order to overcome the flooding phenomena inside PEM fuel cell. Despite the importance impact of the surface wettability of both channel and GDL surface characteristics especially for the cathode side on the water management, few experimental studies have been conducted to investigate the effect of the two-phase flow in cathode gas flow channel and their crucial role. The work presented in this thesis covers contributions that provide insight, not only into the investigation of the effects of hydrophobic cathode GDL and cathode gas flow channels, on water removal, two phase flow inside the channel, and on PEM fuel cell performance, but also the superhydrophobic and superhydrophilic GDLs and gas flow channels effects. Further, the effects of a novel GDL designs with sandwich and gradient wettability with driving capillary force through GDL plane have been investigated. Two-phase flow especially in the cathode gas flow field channels of PEM fuel cell has a crucial role on water removal. Hence, in this research, ex-situ investigations of the effects of channels with different surface wettability; superhydrophobic, hydrophobic, slightly hydrophobic, and superhydrophilic on the two-phase flow characteristics have been tested and visualized at room temperature. Pressure drop measurements and two-phase flow visualization have been carried out using high speed camera. The effect of the various coating materials on graphite and GDL surface morphology, roughness, static contact angle (θ), and sliding contact angle (α) have been investigated using scanning electron microscopy (SEM), Profilometry, and sessile drop technique, respectively. It has been observed that the two-phase flow resistance is considerably affected by surface wettability of the channels. Further, the overall cell performance can be improved by superhydrophobic gas flow channels mainly at high current density over slightly hydrophobic and superhydrophilic cases tested. In addition, sandwich wettability GDL has been coated with a silica particle/ Polydimethylsiloxane (PDMS)…

Subjects/Keywords: Mechanical Engineering PEM Fuel Cell Water Management surface wettability; Fluid Dynamics Materials Engineering

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APA (6^th Edition):

Al Shakhshir, S. (2012). Surface Wettability Impact on Water Management in PEM Fuel Cell. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Al Shakhshir, Saher. “Surface Wettability Impact on Water Management in PEM Fuel Cell.” 2012. Thesis, University of Waterloo. Accessed January 19, 2021. http://hdl.handle.net/10012/7095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Al Shakhshir, Saher. “Surface Wettability Impact on Water Management in PEM Fuel Cell.” 2012. Web. 19 Jan 2021.

Vancouver:

Al Shakhshir S. Surface Wettability Impact on Water Management in PEM Fuel Cell. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10012/7095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Al Shakhshir S. Surface Wettability Impact on Water Management in PEM Fuel Cell. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/7095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Akron

10. Atefi, Ehsan. Aqueous Biphasic 3D Cell Culture Micro-Technology.

Degree: PhD, Biomedical Engineering, 2015, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692

► Three-dimensional (3D) cell culture technologies have gained a considerable momentum in compound screening applications to identify novel anti-cancer drugs. Increasing evidence shows substantial differences between… (more)

▼ Three-dimensional (3D) cell culture technologies have gained a considerable momentum in compound screening applications to identify novel anti-cancer drugs. Increasing evidence shows substantial differences between responses of cancer cells to drug compounds in monolayer cultures (2D) traditionally used in drug discovery and in vivo during preclinical tests. 3D cell cultures more closely resemble tumors in terms of close cell-cell and cell-extracellular matrix interactions, non-uniform distribution of soluble factors, and presence of hypoxic cells. As such, they provide a relevant tumor model to elicit more realistic responses from cells treated with drugs. Screening of libraries of compounds to identify novel drugs requires high throughput 3D culture platforms that produce consistently sized cancer cell spheroids and allow convenient drug testing and analysis of cellular responses.In this study, we introduce a novel, automated technology for 3D culture of cancer cell spheroids in a high throughput format. Aqueous two-phase systems (ATPS) are used for producing spheroids with robotic tools and standard equipment. ATPS are formed by mixing appropriate mass concentrations of two biocompatible polymers such as dextran (DEX) and polyethylene glycol (PEG). A nano-liter drop of the denser aqueous DEX phase containing cancer cells is robotically dispensed into each well of a non-adherent 96-well plate containing the immersion PEG phase solution. A round drop containing cells forms at the bottom of the well while overlaid with the aqueous PEG phase. Cells remain in the DEX drop and form a spheroid, which receives nutrients from the immersion phase through diffusion into the drop.The fidelity of the ATPS spheroid culture technology depends on favorable partition of cells to the DEX drop. We investigate partition of cancer cells in ATPS and demonstrate the effect of interfacial tension between the two aqueous phases on the distribution of cells in ATPS. To facilitate this study, we determine ultralow interfacial tensions of ATPS using an axisymmetric drop shape analysis (ADSA) methodology with sessile and pendant drops and develop mathematical criteria for reliable measurements. To develop a fundamental understanding of the role of interfacial tension of ATPS in cell partition, we develop a theoretical model to predict the energy associated with displacement of a particle (cell) in ATPS. This model, which also uses our contact angle measurements with ATPS/cell systems as an input, shows that a very small interfacial tension, i.e., on the scale of ~30 µJ/m2, results in a minimum free energy when cells locate in the bottom DEX phase, corroborating with our experimental cell partition data and spheroid formation with ATPS. Finally, the utility of this new technology for compound screening is demonstrated by high throughput testing of several anti-cancer drugs against spheroids of skin and breast cancer cells. Incorporating this robotic technology in the oncology drug discovery pipeline will expedite discovery of novel… Advisors/Committee Members: Tavana, Hossein (Committee Chair).

Subjects/Keywords: Biomedical Engineering; 3D Cell Culture, Aqueous Two Phase Systems, Anti-cancer Screening, Interfacial Tension, Surface Tension, Contact Angle, Cell Surface Property, Cancer Cell Fractionation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Atefi, E. (2015). Aqueous Biphasic 3D Cell Culture Micro-Technology. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692

Chicago Manual of Style (16^th Edition):

Atefi, Ehsan. “Aqueous Biphasic 3D Cell Culture Micro-Technology.” 2015. Doctoral Dissertation, University of Akron. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692.

MLA Handbook (7^th Edition):

Atefi, Ehsan. “Aqueous Biphasic 3D Cell Culture Micro-Technology.” 2015. Web. 19 Jan 2021.

Vancouver:

Atefi E. Aqueous Biphasic 3D Cell Culture Micro-Technology. [Internet] [Doctoral dissertation]. University of Akron; 2015. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692.

Council of Science Editors:

Atefi E. Aqueous Biphasic 3D Cell Culture Micro-Technology. [Doctoral Dissertation]. University of Akron; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692

UCLA

11. Hantuli, Mohammed Mazen. The Optimization of Intestinal Epithelial Stem Cell Growth using a Parabolic Response Surface (PRS).

Degree: Bioengineering, 2016, UCLA

URL: http://www.escholarship.org/uc/item/0t1882jq

► Culturing human intestinal stem cells from individual patient samples holds much promise within the realm of research or therapeutic applications. However, performing in-vitro experiments require… (more)

Subjects/Keywords: Biomedical engineering; Biology; cell growth optimization; intestinal stem cells; Lgr5; parabolic response surface; spheroids

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hantuli, M. M. (2016). The Optimization of Intestinal Epithelial Stem Cell Growth using a Parabolic Response Surface (PRS). (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/0t1882jq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hantuli, Mohammed Mazen. “The Optimization of Intestinal Epithelial Stem Cell Growth using a Parabolic Response Surface (PRS).” 2016. Thesis, UCLA. Accessed January 19, 2021. http://www.escholarship.org/uc/item/0t1882jq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hantuli, Mohammed Mazen. “The Optimization of Intestinal Epithelial Stem Cell Growth using a Parabolic Response Surface (PRS).” 2016. Web. 19 Jan 2021.

Vancouver:

Hantuli MM. The Optimization of Intestinal Epithelial Stem Cell Growth using a Parabolic Response Surface (PRS). [Internet] [Thesis]. UCLA; 2016. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/0t1882jq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hantuli MM. The Optimization of Intestinal Epithelial Stem Cell Growth using a Parabolic Response Surface (PRS). [Thesis]. UCLA; 2016. Available from: http://www.escholarship.org/uc/item/0t1882jq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Michigan

12. Karamchand, Leshern. Modulation of Signaling and Intracellular Trafficking Pathways by Surface-Engineered Hydrogel Nanoparticles in Tumor Cells.

Degree: PhD, Chemical Biology, 2014, University of Michigan

URL: http://hdl.handle.net/2027.42/110348

► Surface engineering of a polyacrylamide (PAA) hydrogel nanoparticle (NP) with the tumor-targeting ligand, F3 peptide (KDEPQRRSARLSAKPAPPKPEPKPKKAPAKKC), confers binding specificity toward Nucleolin overexpressing tumor cells (9L… (more)

▼ Surface engineering of a polyacrylamide (PAA) hydrogel nanoparticle (NP) with the tumor-targeting ligand, F3 peptide (KDEPQRRSARLSAKPAPPKPEPKPKKAPAKKC), confers binding specificity toward Nucleolin overexpressing tumor cells (9L rat gliosarcoma, and MDA-MB-435 human breast adenocarcinoma). In this study, the endocytic internalization, and intracellular trafficking of the non-targeted PAA-NPs (NTNPs), and F3-targeted PAA-NPs (F3NPs) in the above-mentioned cell lines, was investigated. Caveolae-mediated internalization of both types of PAA-NPs peaked at 2 hours post-delivery, although internalization of the NTNPs was ~2-fold greater than for the F3NPs. In contrast, clathrin-mediated internalization of both types of PAA-NPs was markedly faster; the NTNPs and F3NPs both reached similar peak colocalization levels with early endosome antigen-1 (EEA1, ~32%) at 30 minutes post-delivery. However, at 60 minutes post-delivery, the NTNPs exhibited faster egress from the early endosomes than the F3NPs, with a concomitant, sharp increase in trafficking to the lysosomes (acidic, degradative vesicles), whereas the F3NPs largely evaded trafficking to the lysosomes. Furthermore, the F3 peptides alone exhibited significantly higher accumulation within the lysosomes than both the NTNPs, and the F3NPs. The p38 Mitogen-Activated Protein Kinases (MAPKs), upon activation, promote (i) internalization of caveolae from the cell membrane, and (ii) rapid trafficking of early endosomes to the lysosomes by directly phosphorylating Caveolin1 and EEA1, respectively. Phospho-proteomic analyses, in MDA-MB-435 cells, revealed that the peak levels of activated p38β and p38δ MAPKs (at 2 hours post-delivery) elicited by the F3 peptides alone, and the NTNPs was ~2-fold greater than by the F3NPs. These data therefore provide compelling evidence that the intracellular trafficking behavior of the F3 peptides, NTNPs and F3NPs are attributable to their differential activation of the p38 MAPKs. Further analysis of the ERK MAPK, JNK MAPK, and Akt pathways revealed that the NTNPs elicit a pro-apoptotic signaling profile, whereas the F3 peptides, and F3NPs elicit proliferative profiles. The findings of this thesis suggest that the design of tumor-targeting nanoparticles also need to consider the MAPK signaling profiles that they elicit on the intended target cell type, due to the influence of the p38 MAPKs, in particular, on endocytic trafficking, and the survival status of the target tumor cell. Advisors/Committee Members: Kopelman, Raoul (committee member), Garcia, George A. (committee member), Glick, Gary D. (committee member), Gafni, Ari (committee member).

Subjects/Keywords: hydrogel nanoparticles; endocytosis and intracellular trafficking; cell signaling pathways; nanoparticle surface engineering; Chemistry; Science

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APA (6^th Edition):

Karamchand, L. (2014). Modulation of Signaling and Intracellular Trafficking Pathways by Surface-Engineered Hydrogel Nanoparticles in Tumor Cells. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/110348

Chicago Manual of Style (16^th Edition):

Karamchand, Leshern. “Modulation of Signaling and Intracellular Trafficking Pathways by Surface-Engineered Hydrogel Nanoparticles in Tumor Cells.” 2014. Doctoral Dissertation, University of Michigan. Accessed January 19, 2021. http://hdl.handle.net/2027.42/110348.

MLA Handbook (7^th Edition):

Karamchand, Leshern. “Modulation of Signaling and Intracellular Trafficking Pathways by Surface-Engineered Hydrogel Nanoparticles in Tumor Cells.” 2014. Web. 19 Jan 2021.

Vancouver:

Karamchand L. Modulation of Signaling and Intracellular Trafficking Pathways by Surface-Engineered Hydrogel Nanoparticles in Tumor Cells. [Internet] [Doctoral dissertation]. University of Michigan; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2027.42/110348.

Council of Science Editors:

Karamchand L. Modulation of Signaling and Intracellular Trafficking Pathways by Surface-Engineered Hydrogel Nanoparticles in Tumor Cells. [Doctoral Dissertation]. University of Michigan; 2014. Available from: http://hdl.handle.net/2027.42/110348

Syracuse University

13. Bandyopadhyay, Debjyoti. Controlling Biofouling by Surface Engineering and Molecular Inhibition.

Degree: PhD, Chemistry, 2011, Syracuse University

URL: https://surface.syr.edu/che_etd/180

► The common theme that binds together all the chapters in this dissertation is anti-biofouling chemistry. By utilizing tools of surface engineering and molecular inhibition, a… (more)

▼ The common theme that binds together all the chapters in this dissertation is anti-biofouling chemistry. By utilizing tools of surface engineering and molecular inhibition, a hypothesis driven, systematic approach of controlling the different forms of biofoulings such as protein adsorption, mammalian cell adhesion and biofilm formation is presented. Chapter 1 provides a brief introduction about topics relevant for understanding the studies presented in subsequent chapters. Chapter 2 reports the synthesis of enantiomerically pure alkanethiols that terminate with different stereoisomer's of sugar alcohols, and the effect of chirality of these polyol-terminated self-assembled monolayers (SAMs) on resisting protein adsorption. Chapter 3 and 4 presents the results on confining adhered mammalian cell (Swiss 3T3 albino fibroblasts) and bacterial (Escherichia coli) biofilm respectively, within micrometer sized cell adhesive patterns of methyl-terminated SAMs, surrounded by bioinert chiral polyol-terminated SAMs. Interestingly, SAMs presenting racemic mixture of enantiomeric alkanethiols were found to confine the adhered mammalian cells or biofilm within the micrometer-sized patterns longer than the SAMs presenting either enantiomer. Chapter 5 presents the synthesis of chiral polyol-terminated alkanephosphonic acids to form SAMs on surface of native metal oxides, particularly TiO₂ and Fe₃O₄, for evaluating the ability of these alkanephosphonate SAMs on TiO2 to resist the adhesion of mammalian cells (Swiss 3T3 albino fibroblasts) and on Fe₃O₄ to solubilize Fe₃O₄ (magnetite) nanoparticles in aqueous media. Another approach of controlling biofouling due to biofilm formation is to develop inhibitors for a class of organic molecules called autoinducers secreted by bacteria, which are responsible for regulating bacterial group behavior such as biofilm formation. Chapter 6 reports the synthesis of derivatives of a class of molecules called brominated furanones, which are known to inhibit biofilm formation in E. coli. Chapter 7 reports the synthesis of novel squarate based molecules named squarylated homoserine lactones (SHLs), which are structural mimics of the bacterial autoinducer molecules called acylated homoserine lactones (AHLs). The synthesized brominated furanone derivatives and SHLs were found to be non-toxic to E. coli and were able to inhibit the biofilm formation by the bacterium. Advisors/Committee Members: Yan-Yeung Luk.

Subjects/Keywords: Anti-biofouling chemistry; Surface engineering; Molecular inhibition; Protein adsorption; Mammalian cell adhesion; Biofilm formation; Chemistry

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APA (6^th Edition):

Bandyopadhyay, D. (2011). Controlling Biofouling by Surface Engineering and Molecular Inhibition. (Doctoral Dissertation). Syracuse University. Retrieved from https://surface.syr.edu/che_etd/180

Chicago Manual of Style (16^th Edition):

Bandyopadhyay, Debjyoti. “Controlling Biofouling by Surface Engineering and Molecular Inhibition.” 2011. Doctoral Dissertation, Syracuse University. Accessed January 19, 2021. https://surface.syr.edu/che_etd/180.

MLA Handbook (7^th Edition):

Bandyopadhyay, Debjyoti. “Controlling Biofouling by Surface Engineering and Molecular Inhibition.” 2011. Web. 19 Jan 2021.

Vancouver:

Bandyopadhyay D. Controlling Biofouling by Surface Engineering and Molecular Inhibition. [Internet] [Doctoral dissertation]. Syracuse University; 2011. [cited 2021 Jan 19]. Available from: https://surface.syr.edu/che_etd/180.

Council of Science Editors:

Bandyopadhyay D. Controlling Biofouling by Surface Engineering and Molecular Inhibition. [Doctoral Dissertation]. Syracuse University; 2011. Available from: https://surface.syr.edu/che_etd/180

University of Colorado

14. Hume, Patrick Scott. Improvement of the Immunoisolation Capacity of PEG Hydrogels through Bioactive Modifications.

Degree: PhD, Chemical & Biochemical Engineering, 2011, University of Colorado

URL: https://scholar.colorado.edu/chbe_gradetds/10

► Cell-based therapies are a promising approach for the treatment of diseases such as Type I diabetes mellitus (TIDM), where endogenous insulin production is restored… (more)

▼ Cell-based therapies are a promising approach for the treatment of diseases such as Type I diabetes mellitus (TIDM), where endogenous insulin production is restored via delivery of insulin-producing beta-cells or islet of Langerhans clusters. Tissue rejection by the host's immune system, however, is a major hurdle limiting the broad use of transplanted tissues, so beta-cell-based therapies require systemic immunosuppression. To reduce this requirement, tissues have been encapsulated within natural and synthetic barrier materials in a process known as immunoisolation. Immunoisolation materials, including poly (ethylene glycol) (PEG) hydrogels, create physical barriers between host immune cells and donor tissue while enabling the diffusion of small molecules like nutrients and oxygen. Unmodified immunoisolation barriers, however, are unable to prevent the diffusion of small cytotoxic molecules, including reactive oxygen species (ROS) (e.g., superoxide) and cytokines. This research investigated strategies to introduce immunoactive modifications to PEG hydrogels for the purpose of improving their immunoisolation capacity. Towards this, a polymerizable superoxide dismutase mimetic (SODm) was covalently tethered within beta-cell-laden hydrogels to significantly increase cell survival following challenges with superoxide, a major inflammatory mediator of the immune response. Next, photoiniferter chemistry was employed to polymerize PEG chains co-functionalized with an apoptosis inducing factor (anti-fas) and a T cell adhesion ligand (ICAM-1) to locally reduce, through apoptosis, the population of T cells, the adaptive immune responder cells implicated in islet transplant rejection. Further, conformal, immunoactive coatings were formed directly on the surfaces of cell-laden PEG hydrogels using a versatile, reactive dip-coating strategy to present a high density of immunoactive signal while maintaining encapsulated cell cytocompatibility. Finally, towards preventing the development of deleterious adaptive immunity altogether, immunosuppressive hydrogels modified with TGF-beta1 and IL-10 were introduced, and their capacity to reduce dendritic cell maturation was highlighted. The immunoactive materials developed within this thesis suggest innovative strategies for the engineering of future immunoisolation barriers to provide localized and targeted protection of transplanted cells. Advisors/Committee Members: Kristi S. Anseth, Christopher N. Bowman, Stephanie J. Bryant.

Subjects/Keywords: Beta cells; Cell Encapsulation; Hydrogel; Immunoisolation; Surface modification; T Cells; Molecular, Cellular, and Tissue Engineering

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APA (6^th Edition):

Hume, P. S. (2011). Improvement of the Immunoisolation Capacity of PEG Hydrogels through Bioactive Modifications. (Doctoral Dissertation). University of Colorado. Retrieved from https://scholar.colorado.edu/chbe_gradetds/10

Chicago Manual of Style (16^th Edition):

Hume, Patrick Scott. “Improvement of the Immunoisolation Capacity of PEG Hydrogels through Bioactive Modifications.” 2011. Doctoral Dissertation, University of Colorado. Accessed January 19, 2021. https://scholar.colorado.edu/chbe_gradetds/10.

MLA Handbook (7^th Edition):

Hume, Patrick Scott. “Improvement of the Immunoisolation Capacity of PEG Hydrogels through Bioactive Modifications.” 2011. Web. 19 Jan 2021.

Vancouver:

Hume PS. Improvement of the Immunoisolation Capacity of PEG Hydrogels through Bioactive Modifications. [Internet] [Doctoral dissertation]. University of Colorado; 2011. [cited 2021 Jan 19]. Available from: https://scholar.colorado.edu/chbe_gradetds/10.

Council of Science Editors:

Hume PS. Improvement of the Immunoisolation Capacity of PEG Hydrogels through Bioactive Modifications. [Doctoral Dissertation]. University of Colorado; 2011. Available from: https://scholar.colorado.edu/chbe_gradetds/10

Université Catholique de Louvain

15. Cheng, Zhe Annie. Biological multi-functionalization and surface nanopatterning of biomaterials.

Degree: 2013, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/137166

►

The aim of biomaterials design is to create an artificial environment that mimics the in vivo extracellular matrix for optimized cell interactions. A precise synergy… (more)

Subjects/Keywords: nanoimprint lithography; surface functionalization; bioactivity; mesenchymal stem cell; focal adhesion; differentiation; tissue engineering

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APA (6^th Edition):

Cheng, Z. A. (2013). Biological multi-functionalization and surface nanopatterning of biomaterials. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/137166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cheng, Zhe Annie. “Biological multi-functionalization and surface nanopatterning of biomaterials.” 2013. Thesis, Université Catholique de Louvain. Accessed January 19, 2021. http://hdl.handle.net/2078.1/137166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cheng, Zhe Annie. “Biological multi-functionalization and surface nanopatterning of biomaterials.” 2013. Web. 19 Jan 2021.

Vancouver:

Cheng ZA. Biological multi-functionalization and surface nanopatterning of biomaterials. [Internet] [Thesis]. Université Catholique de Louvain; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2078.1/137166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cheng ZA. Biological multi-functionalization and surface nanopatterning of biomaterials. [Thesis]. Université Catholique de Louvain; 2013. Available from: http://hdl.handle.net/2078.1/137166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kentucky

16. Cahall, Calvin Frank. SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION.

Degree: 2018, University of Kentucky

URL: https://uknowledge.uky.edu/cme_etds/93

► Surface functionalization of living cells for cell therapeutics has gained substantial momentum in the last two decades. From encapsulating islets of Langerhans, to cell laden… (more)

▼ Surface functionalization of living cells for cell therapeutics has gained substantial momentum in the last two decades. From encapsulating islets of Langerhans, to cell laden gels for tissue scaffolds, to individual cell encapsulation in thin hydrogels, to surface adhesives and inert surface camouflage, modification of living cell surfaces has a wide array of important applications. Here we use hydrogel encapsulation of individual cells as a mode of protection from mechanical forces for high throughput cell printing, and chemical stimuli for the isolation of rare cells in blood. In the first study, we review methods of surface functionalization and establish a metric of potential target biomarkers for circulating tumor cell (CTC) isolation. For extended applications in cancer detection through a fluid biopsy, common surface antigen densities were quantitatively assessed in relation to peripheral blood mononuclear cells (PBMCs) for potential targets of cell specific encapsulation. We then look to commercialization of our process after considering biopsy volumes and cell therapy dose sizes. Undesired batch-to-batch variation in our in-house synthesized photo-initiator could be eliminated by the use of fluorescein, a commercial fluorochrome of similar initiating power to our current eosin initiating system. Fluorescence and hydrogel generation were compared indicating a fluorescein conjugate has comparable power to that of our in-house conjugated eosin. Parameters involving the number of cells and fluid volumes processed were then analyzed systematically. Key parameters were studied to determine optimal equipment and protocol for clinically relevant batch sizes. The final study looks at the mechanical protection provided by thin hydrogel encapsulation. With growing interests in 3D bioprinting and goals of viable whole organ printing for transplant, high resolution and high throughput printing is a growing need. 3D bioprinting presents intense mechanical stimuli in the process that cells must endure. Here we analyze how hydrogel encapsulation reinforces the cellular membrane allowing cells to withstand the damaging forces associated with bioprinting.

Subjects/Keywords: cell encapsulation; bioprinting; photopolymerization; surface polymerization; isolation; Molecular, Cellular, and Tissue Engineering; Polymer Science

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APA (6^th Edition):

Cahall, C. F. (2018). SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/cme_etds/93

Chicago Manual of Style (16^th Edition):

Cahall, Calvin Frank. “SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION.” 2018. Doctoral Dissertation, University of Kentucky. Accessed January 19, 2021. https://uknowledge.uky.edu/cme_etds/93.

MLA Handbook (7^th Edition):

Cahall, Calvin Frank. “SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION.” 2018. Web. 19 Jan 2021.

Vancouver:

Cahall CF. SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION. [Internet] [Doctoral dissertation]. University of Kentucky; 2018. [cited 2021 Jan 19]. Available from: https://uknowledge.uky.edu/cme_etds/93.

Council of Science Editors:

Cahall CF. SURFACE FUNCTIONALIZATION VIA PHOTOINITIATED RADICAL POLYMERIZATION FOR RARE CELL ISOLATION AND MECHANICAL PROTECTION. [Doctoral Dissertation]. University of Kentucky; 2018. Available from: https://uknowledge.uky.edu/cme_etds/93

Cleveland State University

17. Vabbilisetty, Pratima. Functional Anchoring Lipids for Drug Delivery Carrier Fabrication and Cell Surface Re-Engineering Applications.

Degree: PhD, College of Sciences and Health Professions, 2014, Cleveland State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=csu1424175323

► For decades, lipid vesicular bodies such as liposomes have been widely used and explored as biomimetic models of cell membranes and as drug/gene delivery carrier… (more)

Subjects/Keywords: Chemistry; Liposomes, Nanoparticles, Cell Surface Re-engineering, Lipids, Click Chemistry, Staudinger Ligation

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APA (6^th Edition):

Vabbilisetty, P. (2014). Functional Anchoring Lipids for Drug Delivery Carrier Fabrication and Cell Surface Re-Engineering Applications. (Doctoral Dissertation). Cleveland State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=csu1424175323

Chicago Manual of Style (16^th Edition):

Vabbilisetty, Pratima. “Functional Anchoring Lipids for Drug Delivery Carrier Fabrication and Cell Surface Re-Engineering Applications.” 2014. Doctoral Dissertation, Cleveland State University. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=csu1424175323.

MLA Handbook (7^th Edition):

Vabbilisetty, Pratima. “Functional Anchoring Lipids for Drug Delivery Carrier Fabrication and Cell Surface Re-Engineering Applications.” 2014. Web. 19 Jan 2021.

Vancouver:

Vabbilisetty P. Functional Anchoring Lipids for Drug Delivery Carrier Fabrication and Cell Surface Re-Engineering Applications. [Internet] [Doctoral dissertation]. Cleveland State University; 2014. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=csu1424175323.

Council of Science Editors:

Vabbilisetty P. Functional Anchoring Lipids for Drug Delivery Carrier Fabrication and Cell Surface Re-Engineering Applications. [Doctoral Dissertation]. Cleveland State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=csu1424175323

Wright State University

18. Spradlin, Thomas Joshua. Process Sequencing for Fatigue Life Extension of Large Scale Laser Peened Components.

Degree: PhD, Engineering PhD, 2011, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1316292716

► Mechanical surface enhancement techniques have been used to successfully increase the fatigue life of metallic components. These techniques impart compressive surface residual stresses that reduce… (more)

▼ Mechanical surface enhancement techniques have been used to successfully increase the fatigue life of metallic components. These techniques impart compressive surface residual stresses that reduce the tensile stresses experienced during service loading. Laser Peening (LP) is a surface enhancement technique that uses high intensity, short duration laser pulses to create plastic shockwaves in metallic components. Experimental investigations and limited simulation studies have been conducted to determine the effects on the fatigue life of simple coupons when key LP parameters are varied. Used primarily in the aerospace and biomedical engineering fields, LP has been limited to industries that can afford its currently high development and processing costs. Reducing these costs requires that LP simulations, which currently require tremendous amounts of computational power and time due to their complexity, become more time effective and user accessible. This work seeks to address these needs by reducing the overall simulation time of large surface LP processing. The first objective of this work is to reduce the simulation time of an individual LP shot by furthering the development of an extended duration explicit Finite Element Analysis method. Error between the new method and the accepted analysis method is calculated to demonstrate the viability of the new approach. The second objective is to reduce the simulation time of large LP patterns. A symmetry cell approach is developed and used to create a base unit of an LP shot pattern, which is used to reproduce the pattern over a large surface. The last objective is to investigate the effects that the sequencing of LP shots within a pattern can have upon the fatigue life of the component. Due to interactions between adjacent LP shots, the most recent shot in an LP pattern will have the largest compressive stresses. By sequencing the shots in a particular order, the fatigue life of a component can be increased over that of a generic pattern. Advisors/Committee Members: Grandhi, Ramana (Advisor).

Subjects/Keywords: Mechanical Engineering; Laser Peening; Finite Element Analysis; Symmetry Cell; Surface Enhancement; Residual Stress

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APA (6^th Edition):

Spradlin, T. J. (2011). Process Sequencing for Fatigue Life Extension of Large Scale Laser Peened Components. (Doctoral Dissertation). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1316292716

Chicago Manual of Style (16^th Edition):

Spradlin, Thomas Joshua. “Process Sequencing for Fatigue Life Extension of Large Scale Laser Peened Components.” 2011. Doctoral Dissertation, Wright State University. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wright1316292716.

MLA Handbook (7^th Edition):

Spradlin, Thomas Joshua. “Process Sequencing for Fatigue Life Extension of Large Scale Laser Peened Components.” 2011. Web. 19 Jan 2021.

Vancouver:

Spradlin TJ. Process Sequencing for Fatigue Life Extension of Large Scale Laser Peened Components. [Internet] [Doctoral dissertation]. Wright State University; 2011. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1316292716.

Council of Science Editors:

Spradlin TJ. Process Sequencing for Fatigue Life Extension of Large Scale Laser Peened Components. [Doctoral Dissertation]. Wright State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1316292716

19. Gerber, Matthew. The Effect of Anode Geometry on Power Output in Microbial Fuel Cells.

Degree: MS, Mechanical Engineering, 2014, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1406120948

► Microbial fuel cells (MFCs) are bio-electrochemical devices that use micro-organisms, predominately bacteria, to directly convert the chemical energy of substrates contained within wastewater to electricity.… (more)

Subjects/Keywords: Engineering; Fluid Dynamics; Microbiology; microbial fuel cell; microstructure; surface shear rate; geometric form

10 more images…

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APA (6^th Edition):

Gerber, M. (2014). The Effect of Anode Geometry on Power Output in Microbial Fuel Cells. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1406120948

Chicago Manual of Style (16^th Edition):

Gerber, Matthew. “The Effect of Anode Geometry on Power Output in Microbial Fuel Cells.” 2014. Masters Thesis, The Ohio State University. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406120948.

MLA Handbook (7^th Edition):

Gerber, Matthew. “The Effect of Anode Geometry on Power Output in Microbial Fuel Cells.” 2014. Web. 19 Jan 2021.

Vancouver:

Gerber M. The Effect of Anode Geometry on Power Output in Microbial Fuel Cells. [Internet] [Masters thesis]. The Ohio State University; 2014. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1406120948.

Council of Science Editors:

Gerber M. The Effect of Anode Geometry on Power Output in Microbial Fuel Cells. [Masters Thesis]. The Ohio State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1406120948

University of Akron

20. Motta, Cecilia Margarida Mendes. Effect of surface functional groups on chondrocyte behavior using molecular gradients.

Degree: MS, Polymer Science, 2016, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1460392374

► Osteoarthritis (OA) is the most common articular disease and the most prevalent condition resulting in disability among the United States adult population. According to the… (more)

▼ Osteoarthritis (OA) is the most common articular disease and the most prevalent condition resulting in disability among the United States adult population. According to the U.S. Department of Health and Human Services, from 2010-2012, 52.5 million (22.7%) of adults aged > 18 years had self-reported doctor-diagnosed arthritis, and 22.7 million (9.8%) reported arthritis-attributable activity limitation, which indicates not only an ethical, but also economic importance of this disease. OA is characterized by progressive loss of articular cartilage and leads to chronic pain and functional restrictions in the affected joint. Although current treatments are successful in some aspects to provide short-term pain relief and recovered joint mobility, their long term benefits remain elusive and there is still no cure for the disease. The limited capacity for treatment is mainly due to the cartilage`s inability to repair itself. Regenerative medicine using tissue-engineered cartilage has the potential to address this issue, but a remaining challenge is the development of a feasible large scale cell expansion process, since during the expansion in monolayer cultures, chondrocytes undergo the process of dedifferentiation. Several surface-engineering approaches with bioactive factors and surface chemistry have been previously studied to look at increasing the interfacial interaction between the materials and cells. This project aimed to study the effects of various concentrations of surface functional groups on chondrocyte behavior. The cell proliferation and phenotype maintenance within continuously variable one-dimensional concentration gradients were examined. This method included fabrication of functionalized gradients by a vapor deposition technique that provided a fast, efficient, and reliable strategy by incorporating a series of concentrations in single substrates. Finally, human primary chondrocytes density and cellular survival were studied as response of amine and hydroxyl terminal groups` concentrations. Advisors/Committee Members: Becker , Matthew (Advisor).

Subjects/Keywords: Polymers; Biomedical Research; Cartilage; tissue engineering; cell expansion; surface functionalization; gradient concentrations

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APA (6^th Edition):

Motta, C. M. M. (2016). Effect of surface functional groups on chondrocyte behavior using molecular gradients. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1460392374

Chicago Manual of Style (16^th Edition):

Motta, Cecilia Margarida Mendes. “Effect of surface functional groups on chondrocyte behavior using molecular gradients.” 2016. Masters Thesis, University of Akron. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1460392374.

MLA Handbook (7^th Edition):

Motta, Cecilia Margarida Mendes. “Effect of surface functional groups on chondrocyte behavior using molecular gradients.” 2016. Web. 19 Jan 2021.

Vancouver:

Motta CMM. Effect of surface functional groups on chondrocyte behavior using molecular gradients. [Internet] [Masters thesis]. University of Akron; 2016. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1460392374.

Council of Science Editors:

Motta CMM. Effect of surface functional groups on chondrocyte behavior using molecular gradients. [Masters Thesis]. University of Akron; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1460392374

Virginia Commonwealth University

21. Wang, Congzhou. INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES.

Degree: PhD, Engineering, 2015, Virginia Commonwealth University

URL: https://doi.org/10.25772/GM7D-GJ08 ; https://scholarscompass.vcu.edu/etd/4067

► Biomolecules on cell surfaces play critical roles in diverse biological and physiological processes. However, conventional bulk scale techniques are unable to clarify the density… (more)

▼ Biomolecules on cell surfaces play critical roles in diverse biological and physiological processes. However, conventional bulk scale techniques are unable to clarify the density and distribution of specific biomolecules in situ on single, living cell surfaces at the micro or nanoscale. In this work, a single cell analysis technique based on Atomic Force Microscopy (AFM) is developed to spatially identify biomolecules and characterize nanomechanical properties on single cell surfaces. The unique advantage of these AFM-based techniques lies in the ability to operate in situ (in a non-destructive fashion) and in real time, under physiological conditions or controlled micro-environments. First, AFM-based force spectroscopy was developed to study the fundamental biophysics of the heparin/thrombin interaction at the molecular level. Based on force spectroscopy, a force recognition mapping strategy was developed and optimized to spatially detect single protein targets on non-biological surfaces. This platform was then translated to the study of complex living cell surfaces. Specific carbohydrate compositions and changes in their distribution, as well as elasticity change were obtained by monitoring Bacillus cells sporulation process. The AFM-based force mapping technique was applied to different cellular systems to develop a cell surface biomolecule library. Nanoscale imaging combined with carbohydrate mapping was used to evaluate inactivation methods and growth temperatures effects on Yersinia pestis surface. A strategy to image cells in real time was coupled with hydrophobicity mapping technique to monitor the effect of antimicrobials (antimicrobial polymer and copper) on Escherichia coli and study their killing mechanisms. The single spore hydrophobicity mapping was used to localize the exosporium structure and potentially reconstruct culture media. The descriptions of cell surface DNA on single human epithelial cells potentially form a novel tool for forensic identification. Overall, these nanoscale tools to detect and assess changes in cell behavior and function over time, either as a result of natural state changes or when perturbed, will further our understanding of fundamental biological processes and lead to novel, robust methods for the analysis of individual cells. Real time analysis of cells can be used for the development of lab-on-chip type assays for drug design and testing or to test the efficacy of antimicrobials. Advisors/Committee Members: Vamsi K. Yadavalli.

Subjects/Keywords: cell surface biomolecules; atomic force microscopy; single cell analysis; bacteria; nanoscale; Bacteriology; Biochemical and Biomolecular Engineering; Biotechnology; Nanoscience and Nanotechnology; Pharmacology

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APA (6^th Edition):

Wang, C. (2015). INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/GM7D-GJ08 ; https://scholarscompass.vcu.edu/etd/4067

Chicago Manual of Style (16^th Edition):

Wang, Congzhou. “INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES.” 2015. Doctoral Dissertation, Virginia Commonwealth University. Accessed January 19, 2021. https://doi.org/10.25772/GM7D-GJ08 ; https://scholarscompass.vcu.edu/etd/4067.

MLA Handbook (7^th Edition):

Wang, Congzhou. “INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES.” 2015. Web. 19 Jan 2021.

Vancouver:

Wang C. INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2015. [cited 2021 Jan 19]. Available from: https://doi.org/10.25772/GM7D-GJ08 ; https://scholarscompass.vcu.edu/etd/4067.

Council of Science Editors:

Wang C. INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES. [Doctoral Dissertation]. Virginia Commonwealth University; 2015. Available from: https://doi.org/10.25772/GM7D-GJ08 ; https://scholarscompass.vcu.edu/etd/4067

University of Kentucky

22. Wu, Pei-Jung. CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY.

Degree: 2019, University of Kentucky

URL: https://uknowledge.uky.edu/cme_etds/104

► The cell plasma membrane is an interactive interface playing an important role in regulating cell-to-cell, cell-to-tissue contact, and cell-to-environment responses. This environment-responsive phospholipid layer consisting… (more)

▼ The cell plasma membrane is an interactive interface playing an important role in regulating cell-to-cell, cell-to-tissue contact, and cell-to-environment responses. This environment-responsive phospholipid layer consisting of multiple dynamically balanced macromolecules, such as membrane proteins, carbohydrate and lipids, is regarded as a promising platform for various surface engineering strategies. Through different chemical modification routes, we are able to incorporate various artificial materials into the cell surface for biomedical applications in small molecule and cellular therapeutics. In this dissertation, we establish two different cell coating techniques for applications of cell-mediated drug delivery and the localization of cell-based therapies to specific tissues. The first part of this dissertation establishes a membrane-associated hydrogel patch for drug delivery. The crosslinking of a grafted polymeric patch from a mammalian cell membrane is achieved through surface-mediated photolithographic polymerization. With the use of photomask, the formation of nanoparticle-loaded PEGDA hydrogel is controlled to deposit various geometric features on photoinitiator-immobilized surfaces. Through microarray patch patterning, we analyzed the influence of processing parameters on the accuracy of polymer patterning on a microarray. We then optimized the patterning approach for the formation of PEGDA patches on live A549 cells. In the second part of this dissertation, we study the use of tissue-adhesive coatings to improve the retention of therapeutic mesenchymal stem cells (MSCs) in the heart following intramyocardial or intravenous injection. MSCs were coated with antibodies against ICAM1 to adhere to CAM-overexpressed endothelium present in the heart following MI. Through intramyocardial or intravenous delivery, we observe higher number of coated cells retained in the heart over uncoated ones, supporting enhanced affinity for the inflamed endothelium near the infarct. We correlate the detachment force of antigen-interacted MSCs by a parallel laminar flow assay with the density of ICAM on the substrate and the density of anti-ICAM on the MSC surface. MSC retention on CAMmodified surfaces or activated HUVECs was significantly increased on antibody-coated groups (~90%) under physiologically hemodynamic forces (< 30dyne/cm2), compared to uncoated MSCs (~20%). Moreover, a dramatic reduction of immune cell quantity was observed after intravenous injection, indicating the enhanced immunoregulatory efficacy by systemically delivering ICAM-adhesive MSCs to the site of inflammation.

Subjects/Keywords: cell surface coating; hydrogel patch; surface-mediated polymerization; antibody incorporation; endothelium targeting; biotin streptavidin affinity; Biochemical and Biomolecular Engineering; Biomaterials; Biomedical Engineering and Bioengineering; Molecular, Cellular, and Tissue Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, P. (2019). CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/cme_etds/104

Chicago Manual of Style (16^th Edition):

Wu, Pei-Jung. “CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY.” 2019. Doctoral Dissertation, University of Kentucky. Accessed January 19, 2021. https://uknowledge.uky.edu/cme_etds/104.

MLA Handbook (7^th Edition):

Wu, Pei-Jung. “CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY.” 2019. Web. 19 Jan 2021.

Vancouver:

Wu P. CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY. [Internet] [Doctoral dissertation]. University of Kentucky; 2019. [cited 2021 Jan 19]. Available from: https://uknowledge.uky.edu/cme_etds/104.

Council of Science Editors:

Wu P. CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY. [Doctoral Dissertation]. University of Kentucky; 2019. Available from: https://uknowledge.uky.edu/cme_etds/104

University of Akron

23. Ni, Liwei. Microfluidic Device for Noninvasive Cell Electrical Stimulation, Extracellular Field Potential Analysis and Surface Charge Detection.

Degree: PhD, Mechanical Engineering, 2020, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1586518282134534

► Electrical properties of cells have been studied to understand the functions and mechanisms of various types of cells. The cell’s overall electricity results from the… (more)

Subjects/Keywords: Biomedical Engineering; Mechanical Engineering; Microfluidic device, Cell analysis, Electrical stimulation, Extracellular field potential, Surface Charge, Resistive Pulse Sensing

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APA (6^th Edition):

Ni, L. (2020). Microfluidic Device for Noninvasive Cell Electrical Stimulation, Extracellular Field Potential Analysis and Surface Charge Detection. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1586518282134534

Chicago Manual of Style (16^th Edition):

Ni, Liwei. “Microfluidic Device for Noninvasive Cell Electrical Stimulation, Extracellular Field Potential Analysis and Surface Charge Detection.” 2020. Doctoral Dissertation, University of Akron. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1586518282134534.

MLA Handbook (7^th Edition):

Ni, Liwei. “Microfluidic Device for Noninvasive Cell Electrical Stimulation, Extracellular Field Potential Analysis and Surface Charge Detection.” 2020. Web. 19 Jan 2021.

Vancouver:

Ni L. Microfluidic Device for Noninvasive Cell Electrical Stimulation, Extracellular Field Potential Analysis and Surface Charge Detection. [Internet] [Doctoral dissertation]. University of Akron; 2020. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1586518282134534.

Council of Science Editors:

Ni L. Microfluidic Device for Noninvasive Cell Electrical Stimulation, Extracellular Field Potential Analysis and Surface Charge Detection. [Doctoral Dissertation]. University of Akron; 2020. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1586518282134534

University of California – Berkeley

24. Fan, Houfu. A multiscale moving contact line theory and its applications on the simulations of dynamic droplet wetting and cell motility on soft substrates.

Degree: Civil and Environmental Engineering, 2014, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/3q10w887

► In this dissertation, a multiscale moving contact line (MMCL) theory is proposed, to simulate liquid droplet spreading, capillary motion and in particular, to study cell… (more)

▼ In this dissertation, a multiscale moving contact line (MMCL) theory is proposed, to simulate liquid droplet spreading, capillary motion and in particular, to study cell motility on the extracellular matrix. The proposed multiscale moving contact line theory combines a coarse-grained contact model (CGCM) with a generalized Gurtin-Murdoch surface elasticity theory, so that it can couple the molecular scale adhesive interaction with the macroscale motion. The intermolecular adhesive force (van der Waals force) separates and levitates the liquid droplet from the supporting solid substrate, such that the proposed MMCL theory can avoid the singularity problem caused by the no-slip condition in the conventional hydrodynamics of moving contact line theory.The proposed MMCL theory is formulated as a variational principle and implemented within a Lagrangian finite element method. Two different implementations with different ways of calculating the contact/adhesion forces, regarding the computational efficiency, are proposed. Numerical examples are presented to illustrate the applicability of the MMCL theory. Several simulations of complete three-dimensional water droplet spreadings upon various elastic substrates are performed. The numerical results are in good agreement with those of molecular dynamics simulations and experiments reported in literature. In addition, the capillary motion around a spherical cap is captured using the MMCL theory. The contact model (CGCM) used in the MMCL theory is initially designed to simulate contact and adhesion at nano or submicro scale. By using a second level coarse graining technique, it can be employed to simulate problems at meso or even macro scales and thus make the MMCL theory available to simulations of cell motility, which is usually at a scale ranging from ten micrometer to several millimeter. At last, the MMCL theory is used to study the interactions between cells and their extracellular matrices. In our framework, a cell is modeled as Nematic liquid crystal or liquid crystal elatomer and the extracellular matrix is treated as an elastic substrate. Cell spreading upon various extracellular substrates are successfully simulated, aiming to improve the understanding of the mechanotransduction mechanism that is in charge of mechanical information exchange between a cell and its surrounding environment. Through the numerical simulations, it is demonstrated that the cell can sense the substrate elasticity in many different ways. In fact, together with a proposed scheme that resembles a linkage between the traction forces and cell substrate elasticity based on experimental observations, self-propelled movement of a cell on the gradient of substrate elasticity (called durotaxis) is successfully captured.

Subjects/Keywords: Mechanics; Computer science; Computer engineering; cell motility; coarse-grained contact; contact angle; moving contact line; multiscale; surface tension

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APA (6^th Edition):

Fan, H. (2014). A multiscale moving contact line theory and its applications on the simulations of dynamic droplet wetting and cell motility on soft substrates. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/3q10w887

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fan, Houfu. “A multiscale moving contact line theory and its applications on the simulations of dynamic droplet wetting and cell motility on soft substrates.” 2014. Thesis, University of California – Berkeley. Accessed January 19, 2021. http://www.escholarship.org/uc/item/3q10w887.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fan, Houfu. “A multiscale moving contact line theory and its applications on the simulations of dynamic droplet wetting and cell motility on soft substrates.” 2014. Web. 19 Jan 2021.

Vancouver:

Fan H. A multiscale moving contact line theory and its applications on the simulations of dynamic droplet wetting and cell motility on soft substrates. [Internet] [Thesis]. University of California – Berkeley; 2014. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/3q10w887.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fan H. A multiscale moving contact line theory and its applications on the simulations of dynamic droplet wetting and cell motility on soft substrates. [Thesis]. University of California – Berkeley; 2014. Available from: http://www.escholarship.org/uc/item/3q10w887

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

25. Buehler, Michelle Kay. Laser texturing of drug eluting stents to improve drug adhesion.

Degree: 2011, Iowa State University

URL: https://lib.dr.iastate.edu/etd/12225

► Drug eluting stents have made significant advances in reducing restenosis over bare metal stents; however, further research is required to decrease the likelihood of late… (more)

Subjects/Keywords: Cardiovascular devices; Drug/cell deliver systems; Drug Eluting Stent; Laser; Medical device manufacturing; Surface Texture; Mechanical Engineering

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APA (6^th Edition):

Buehler, M. K. (2011). Laser texturing of drug eluting stents to improve drug adhesion. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/12225

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Buehler, Michelle Kay. “Laser texturing of drug eluting stents to improve drug adhesion.” 2011. Thesis, Iowa State University. Accessed January 19, 2021. https://lib.dr.iastate.edu/etd/12225.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Buehler, Michelle Kay. “Laser texturing of drug eluting stents to improve drug adhesion.” 2011. Web. 19 Jan 2021.

Vancouver:

Buehler MK. Laser texturing of drug eluting stents to improve drug adhesion. [Internet] [Thesis]. Iowa State University; 2011. [cited 2021 Jan 19]. Available from: https://lib.dr.iastate.edu/etd/12225.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Buehler MK. Laser texturing of drug eluting stents to improve drug adhesion. [Thesis]. Iowa State University; 2011. Available from: https://lib.dr.iastate.edu/etd/12225

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Bordeaux I

26. Cheng, Zhe Annie. Biological multi-functionalization and surface nanopatterning of biomaterials : Multi-fonctionnalisation et micro-, nanostructuration de la surface de biomatériaux.

Degree: Docteur es, Polymères, 2013, Université de Bordeaux I

URL: http://www.theses.fr/2013BOR15202

►

Le but de la conception d’un biomatériau est de mimer les modèles qui puissent être représentatifs de la matrice extracellulaire (MEC) existant in vivo. Cet… (more)

▼

Le but de la conception d’un biomatériau est de mimer les modèles qui puissent être représentatifs de la matrice extracellulaire (MEC) existant in vivo. Cet objectif peut être atteint en associant une combinaison de cellules et des facteurs biologiques à un biomatériau sur lequel ces cellules peuvent se développer pour reconstruire le tissu natif. Dans cet étude, nous avons crée des surfaces bioactives nanostructurées en combinant la nanolithographie et la fonctionnalisation de surface, en greffant un peptide RGD ou BMP-2 (bone morphogenetic protein 2). Nous avons étudié l’effet de cette nanodistribution sur le comportement des cellules souches mésenchymateuses en analysant leur adhésion et différentiation. Nous notons que la nanodistribution des peptides induit une bioactivité qui a un impact sur l’organisation du cytosquelette, la conformation des fibres de stresse de l’actin, la maturation des adhésions focales (AFs), et le commitment des cellules souches. En particulier, l’aire, la distribution, et la conformation des AFs sont affectes par la présence des nanopatterns. En plus, le RGD et le BMP-2 changent le comportement cellulaire par des voies et des mécanismes différents en variant l’organisation des cellules souches et la maturation de leurs AFs. La nanodistribution influence de façon évidente les cellules souches en modifiant leur comportement (adhésion et différenciation) ce qui a contribué et ce qui contribuera à améliorer la compréhension des interactions des cellules avec la MEC.

The aim of biomaterials design is to create an artificial environment that mimics the in vivo extracellular matrix for optimized cell interactions. A precise synergy between the scaffolding material, bioactivity, and cell type must be maintained in an effective biomaterial. In this work, we present a technique of nanofabrication that creates chemically nanopatterned bioactive silicon surfaces for cell studies. Using nanoimprint lithography, RGD and mimetic BMP-2 peptides were covalently grafted onto silicon as nanodots of various dimensions, resulting in a nanodistribution of bioactivity. To study the effects of spatially distributed bioactivity on cell behavior, mesenchymal stem cells (MSCs) were cultured on these chemically modified surfaces, and their adhesion and differentiation were studied. MSCs are used in regenerative medicine due to their multipotent properties, and well-controlled biomaterial surface chemistries can be used to influence their fate. We observe that peptide nanodots induce differences in MSC behavior in terms of cytoskeletal organization, actin stress fiber arrangement, focal adhesion (FA) maturation, and MSC commitment in comparison with homogeneous control surfaces. In particular, FA area, distribution, and conformation were highly affected by the presence of peptide nanopatterns. Additionally, RGD and mimetic BMP-2 peptides influenced cellular behavior through different mechanisms that resulted in changes in cell spreading and FA maturation. These findings have remarkable implications that…

Advisors/Committee Members: Durrieu, Marie-Christine (thesis director), Jonas, Alain M. (thesis director).

Subjects/Keywords: Lithographie par nanoimpression; Fonctionnalisation de surface; Bioactivité; Cellule souche mésenchymateuse; Adhésion focale; Différenciation; Ingénierie tissulaire; Nanoimprint lithography; Surface functionalization; Bioactivity; Mesenchymal stem cell; Focal adhesion; Differentiation; Tissue engineering

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APA (6^th Edition):

Cheng, Z. A. (2013). Biological multi-functionalization and surface nanopatterning of biomaterials : Multi-fonctionnalisation et micro-, nanostructuration de la surface de biomatériaux. (Doctoral Dissertation). Université de Bordeaux I. Retrieved from http://www.theses.fr/2013BOR15202

Chicago Manual of Style (16^th Edition):

Cheng, Zhe Annie. “Biological multi-functionalization and surface nanopatterning of biomaterials : Multi-fonctionnalisation et micro-, nanostructuration de la surface de biomatériaux.” 2013. Doctoral Dissertation, Université de Bordeaux I. Accessed January 19, 2021. http://www.theses.fr/2013BOR15202.

MLA Handbook (7^th Edition):

Cheng, Zhe Annie. “Biological multi-functionalization and surface nanopatterning of biomaterials : Multi-fonctionnalisation et micro-, nanostructuration de la surface de biomatériaux.” 2013. Web. 19 Jan 2021.

Vancouver:

Cheng ZA. Biological multi-functionalization and surface nanopatterning of biomaterials : Multi-fonctionnalisation et micro-, nanostructuration de la surface de biomatériaux. [Internet] [Doctoral dissertation]. Université de Bordeaux I; 2013. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2013BOR15202.

Council of Science Editors:

Cheng ZA. Biological multi-functionalization and surface nanopatterning of biomaterials : Multi-fonctionnalisation et micro-, nanostructuration de la surface de biomatériaux. [Doctoral Dissertation]. Université de Bordeaux I; 2013. Available from: http://www.theses.fr/2013BOR15202

27. HE LIJUAN. Engineering aggregates with chemical linkers for tissue engineering application.

Degree: 2006, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/15335

Subjects/Keywords: cell surface modification; chemical linker; cell aggregates; suspension culture; fluorescence; tissue engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

LIJUAN, H. (2006). Engineering aggregates with chemical linkers for tissue engineering application. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/15335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

LIJUAN, HE. “Engineering aggregates with chemical linkers for tissue engineering application.” 2006. Thesis, National University of Singapore. Accessed January 19, 2021. http://scholarbank.nus.edu.sg/handle/10635/15335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

LIJUAN, HE. “Engineering aggregates with chemical linkers for tissue engineering application.” 2006. Web. 19 Jan 2021.

Vancouver:

LIJUAN H. Engineering aggregates with chemical linkers for tissue engineering application. [Internet] [Thesis]. National University of Singapore; 2006. [cited 2021 Jan 19]. Available from: http://scholarbank.nus.edu.sg/handle/10635/15335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIJUAN H. Engineering aggregates with chemical linkers for tissue engineering application. [Thesis]. National University of Singapore; 2006. Available from: http://scholarbank.nus.edu.sg/handle/10635/15335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New Mexico

28. Workman, Michael J. A Study of Iron-Nitrogen-Carbon Fuel Cell Catalysts: Chemistry – Nanostructure – Performance.

Degree: Nanoscience and Microsystems, 2017, University of New Mexico

URL: https://digitalrepository.unm.edu/nsms_etds/36

► Fuel cells have the potential to be a pollution-free, low-cost, and energy efficient alternative to the internal combustion engine for transportation and small-scale stationary… (more)

▼ Fuel cells have the potential to be a pollution-free, low-cost, and energy efficient alternative to the internal combustion engine for transportation and small-scale stationary power applications. The current state of fuel cell technology has already achieved two of these three lofty goals. The remaining barrier to wide-scale deployment is the high cost, which is primarily caused by dependence on large amounts of platinum to catalyze the energy conversion reactions. To overcome this barrier and facilitate the integration of fuel cells into mainstream applications, research into a new class of catalyst materials that do not require platinum is needed. There has been a significant amount of research effort directed toward the development of platinum-group metal free (PGM-free) catalysts, yet there is a lack of consensus on both the engineering parameters necessary to improve the technology and the fundamental science that would facilitate rational design. I have engaged in research on PGM-free catalysts based on inexpensive and abundant reagents, specifically: nicarbazin and iron. Catalysts made from these precursors have previously proven to be among the best PGM-free catalysts, but their continued advancement suffered from the same lack of understanding that besets all catalysts in this class. The work I have performed address both engineering concerns and fundamental underlying principles. I present results demonstrating correlations between physical structure, chemical speciation, and synthesis parameters, as well as addressing active site chemistry and likely locations. My research presented herein introduces new morphology analysis techniques and elucidates several key structure-to-property characteristics of catalysts derived from iron and nicarbazin. I discuss the development and application of a new length-scale specific surface analysis technique that allows for analysis of well-defined size ranges from a few nm to several microns. The existing technique of focused ion beam tomography is modified and optimized for platinum-group metal free catalyst layers, facilitating direct observation of catalyst integration into catalyst layers. I present evidence supporting the hypothesis that atomically dispersed iron coordinated with nitrogen are the dominant active sites in these catalysts. Further, that the concentration of surface oxides in the carbon structure, which can be directly influenced by synthesis parameters, correlates with both the concentration of active sites in the material and with fuel cell performance. Catalyst performance is hindered by the addition of carbon nanotubes and by the presence of metallic iron. Evidence consistent with the catalytic active sites residing in the graphitic plane is also presented. Advisors/Committee Members: Plamen Atanassov, Kateryna Artyushkova, Fernando Garzon, Scott Calabrese-Barton.

Subjects/Keywords: Fuel cell; catalysis; PGM-free; structure-to-property; energy; surface analysis; Catalysis and Reaction Engineering; Materials Science and Engineering; Nanoscience and Nanotechnology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Workman, M. J. (2017). A Study of Iron-Nitrogen-Carbon Fuel Cell Catalysts: Chemistry – Nanostructure – Performance. (Doctoral Dissertation). University of New Mexico. Retrieved from https://digitalrepository.unm.edu/nsms_etds/36

Chicago Manual of Style (16^th Edition):

Workman, Michael J. “A Study of Iron-Nitrogen-Carbon Fuel Cell Catalysts: Chemistry – Nanostructure – Performance.” 2017. Doctoral Dissertation, University of New Mexico. Accessed January 19, 2021. https://digitalrepository.unm.edu/nsms_etds/36.

MLA Handbook (7^th Edition):

Workman, Michael J. “A Study of Iron-Nitrogen-Carbon Fuel Cell Catalysts: Chemistry – Nanostructure – Performance.” 2017. Web. 19 Jan 2021.

Vancouver:

Workman MJ. A Study of Iron-Nitrogen-Carbon Fuel Cell Catalysts: Chemistry – Nanostructure – Performance. [Internet] [Doctoral dissertation]. University of New Mexico; 2017. [cited 2021 Jan 19]. Available from: https://digitalrepository.unm.edu/nsms_etds/36.

Council of Science Editors:

Workman MJ. A Study of Iron-Nitrogen-Carbon Fuel Cell Catalysts: Chemistry – Nanostructure – Performance. [Doctoral Dissertation]. University of New Mexico; 2017. Available from: https://digitalrepository.unm.edu/nsms_etds/36

29. Ali, Maryam. Dynamic micro-3D-printed substrates for characterizing cellular responses to topography.

Degree: PhD, Biomedical Engineering, 2014, University of Texas – Austin

URL: http://hdl.handle.net/2152/26070

► Cell cultures provide researchers the opportunity to observe cell behavior in response to specific, well-defined environmental cues, leading to insights that enable better engineering design… (more)

▼ Cell cultures provide researchers the opportunity to observe cell behavior in response to specific, well-defined environmental cues, leading to insights that enable better engineering design for tissue culture and other biomedical applications. Chemical and electrical stimuli have been successfully applied to cultured cells to approximate aspects of the dynamic conditions experienced in vivo. However, in vitro topographical cues have mostly been limited to static substrates that do not subject cells to the dynamic conditions they experience in vivo when tissue remodels during development and wound healing. Delivering dynamic topographical cues to cultured cells can answer long-standing questions about mechanisms of cell morphology changes. Such capabilities could also facilitate engineering of wound-healing matrices and nerve guidance conduits by promoting migration of cells and providing directional guidance to cellular processes. This dissertation describes the development of approaches for introducing in situ topographical cues to cell cultures and inducing responses such as neurite guidance and cell alignment. Both strategies undertaken in this work make use of multiphoton-promoted photochemistry to print and manipulate three-dimensional microscopic protein hydrogel structures. In one approach, a technique referred to as micro-3D printing, topographical guidance cues are printed in the proximity of cultured cells to guide the growth of cellular processes. By translating a tightly-focused pulsed laser beam through a printing reagent solution flooding cultured cells, features are printed that provide physical guidance to extending neurites from NG108-15 cells, a neuronal model cell type. In another approach, an innovative technique known as micro-3D imprinting is developed for producing micrometer-scale depressions on the surfaces of photoresponsive protein hydrogels. The impact of various experimental parameters on topographical feature dimensions is characterized. Micro-3D imprinting is used to introduce dynamic topographical changes on a cell culture substrate, demonstrating that NIH-3T3 cells, a fibroblast cell model, alter their morphology and alignment in response to the introduction of a grooved surface topography. This set of approaches introduces new tools to the repertoire of cell biologists for exploring the behavior of cells growing in a spatio-temporally dynamic environment, opening possibilities for studies of cellular behavior in conditions that may better reflect environments cells experience in vivo. Advisors/Committee Members: Shear, Jason B. (advisor).

Subjects/Keywords: Biomedical engineering; Biomaterials; Cell biology; Multiphoton; 3D printing; Cell environment; Cell-surface interaction; Substrate topography

…of cell morphology changes. Such capabilities could also facilitate engineering of wound… …142 Figure 4.6: A montage of a cell migrating over a grooved surface… …disposable polystyrene as the preferred cell culture surface (Cooper 1961; Ryan 2008)… …treatment produce a surface that supports a wide variety of cell types [12]. Coating the… …surface with polypeptides or glycoproteins can also improve cell adhesion [13]. 2…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ali, M. (2014). Dynamic micro-3D-printed substrates for characterizing cellular responses to topography. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/26070

Chicago Manual of Style (16^th Edition):

Ali, Maryam. “Dynamic micro-3D-printed substrates for characterizing cellular responses to topography.” 2014. Doctoral Dissertation, University of Texas – Austin. Accessed January 19, 2021. http://hdl.handle.net/2152/26070.

MLA Handbook (7^th Edition):

Ali, Maryam. “Dynamic micro-3D-printed substrates for characterizing cellular responses to topography.” 2014. Web. 19 Jan 2021.

Vancouver:

Ali M. Dynamic micro-3D-printed substrates for characterizing cellular responses to topography. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152/26070.

Council of Science Editors:

Ali M. Dynamic micro-3D-printed substrates for characterizing cellular responses to topography. [Doctoral Dissertation]. University of Texas – Austin; 2014. Available from: http://hdl.handle.net/2152/26070

Cleveland State University

30. Jensen, Rebecca Leah. Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model.

Degree: MSin Chemical Engineering, Fenn College of Engineering, 2013, Cleveland State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419

► Under this research, live cell imaging of osteoblast-like marrow stromal cells has been carried out on polished and nanotextured (NaOH-etched) medical-grade titanium alloy (Ti-6Al-4V) surfaces… (more)

▼ Under this research, live cell imaging of osteoblast-like marrow stromal cells has been carried out on polished and nanotextured (NaOH-etched) medical-grade titanium alloy (Ti-6Al-4V) surfaces to examine cellular adhesion and migration. The purpose of this research was to:1)Build and assemble suitable hardware and software to conduct live cell imaging in a micro-incubator over an extended period of time.2)Monitor and record live osteoblast-like marrow stromal cells on polished and NaOH-etched titanium alloy surfaces from cell inoculation to about one week of culture. 3)Measure location, area and perimeter of individual cells as a function of time, and examine if, as compared / contrasted with the polished titanium surface, that the NaOH-etched titanium surface promotes adhesion and migration of cells.This was achieved by describing the mobility, morphology and overall behavior of osteoblast-like marrow stromal cells. During the cell growth cycles, data generated from image analysis included the cells’ center of mass (X,Y), their area, perimeter and shape as a function of incubation time. From the change in center of mass after each 15-minute interval, the real time speed of the cells was obtained. Major observations to support comparison studies between the surfaces determined that compared with polished titanium, NaOH-etched titanium promotes cellular filopodia growth, thus, promotes attachment. Filopodia provide cellular anchoring support and when prevalent, make cells more angular in shape. The median aspect ratio (length / width) of cells was found to be 1.38 on polished and 2.36 on NaOH-etched titanium. This, in addition to lower mean circularity shape factor values of 0.26 ± 0.03 on polished and 0.11 ± 0.01 on NaOH-etched titanium imply that the nanotextured surface promotes growth of cells more anchored to the substrate. This is also confirmed by increased perimeters of cells found on the NaOH-etched surface (950.92 ± 84.88 μm) compared with perimeter lengths of cells on the polished surface (668.55 ± 22.55 μm). Lastly, lower real time cell migration speed of 9 ± 1.2 μm / hour also suggests improved attachment of cells on NaOH-etched titanium with values speeds on the polished surface being 65 ± 19 μm / hour. Advisors/Committee Members: Tewari, Surendra (Committee Chair).

Subjects/Keywords: Biomedical Engineering; marrow stromal cells; titanium implant; nano-texture; NaOH-etched surface; cellular attachment; shape factor; live cell imaging; micro-incubator; osteoblast cells; cell migration; cell morphology; aspect ratio; in vitro; green fluorescent protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jensen, R. L. (2013). Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model. (Masters Thesis). Cleveland State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419

Chicago Manual of Style (16^th Edition):

Jensen, Rebecca Leah. “Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model.” 2013. Masters Thesis, Cleveland State University. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419.

MLA Handbook (7^th Edition):

Jensen, Rebecca Leah. “Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model.” 2013. Web. 19 Jan 2021.

Vancouver:

Jensen RL. Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model. [Internet] [Masters thesis]. Cleveland State University; 2013. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419.

Council of Science Editors:

Jensen RL. Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model. [Masters Thesis]. Cleveland State University; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419

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Open Access Theses and Dissertations