You searched for subject:(Cell differentiation).
Showing records 1 – 30 of
1077 total matches.
◁ [1] [2] [3] [4] [5] … [36] ▶
1.
Mori, Megumi.
Gene Expression Changes Associated with the Differentiation
of Mouse Embryonic Stem Cells to Primordial Germ Cell-Like
Cells.
Degree: Department of Molecular Biology, Cell Biology and
Biochemistry, 2018, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:792693/ 
► Embryonic stem cells (ESCs) are found early in the developing embryo and from this initial population, all cells in the body are eventually derived. A…
(more)
▼ Embryonic stem cells (ESCs) are found early in the
developing embryo and from this initial population, all cells in
the body are eventually derived. A hallmark characteristic of ESCs
is their pluripotent nature, which is established to a large extent
by transcriptional regulation. The core transcription factor
network commonly referred to in stem
cell pluripotency includes
Oct3/4, Sox2 and Nanog which then contribute to a vast protein
interaction network. The process by which embryonic stem cells
become differentiated into cells with novel characteristics is
tightly regulated and specific. In the past several decades, there
has been great interest regarding how mammalian ESCs might be
cultured in vitro to promote the derivation of
cell types for
research as well as medical endeavors. Primordial germ cells, the
precursors of gametes, are an especially interesting
subject for
differentiation as they are linked to ESCs via their reinstated
pluripotency upon fertilization to divide and constitute a new
organism. This thesis describes the theory and experimental
methodology necessary to derive primordial germ
cell-like cells
(PGCLCs) from mouse embryonic stem cells (ESCs). Throughout this
process, the optimal expansion and culture conditions of ESCs,
epiblast-like cells (EpiLCs) and PGCLCs were implemented. A major
initial goal was to assess the efficacy of
differentiation via
expected changes in gene expression throughout each
differentiation
step. Establishing this in vitro model system will then allow us to
answer mechanistic questions about the how subunits of the core
transcription factor TFIID complex may contribute to the
maintenance of ESC pluripotency through the transcriptional network
and how they change to regulate the pathway by which PGCLCs are
specified.
Advisors/Committee Members: Freiman, Richard (Advisor).
Subjects/Keywords: Cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mori, M. (2018). Gene Expression Changes Associated with the Differentiation
of Mouse Embryonic Stem Cells to Primordial Germ Cell-Like
Cells. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792693/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mori, Megumi. “Gene Expression Changes Associated with the Differentiation
of Mouse Embryonic Stem Cells to Primordial Germ Cell-Like
Cells.” 2018. Thesis, Brown University. Accessed April 18, 2021.
https://repository.library.brown.edu/studio/item/bdr:792693/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mori, Megumi. “Gene Expression Changes Associated with the Differentiation
of Mouse Embryonic Stem Cells to Primordial Germ Cell-Like
Cells.” 2018. Web. 18 Apr 2021.
Vancouver:
Mori M. Gene Expression Changes Associated with the Differentiation
of Mouse Embryonic Stem Cells to Primordial Germ Cell-Like
Cells. [Internet] [Thesis]. Brown University; 2018. [cited 2021 Apr 18].
Available from: https://repository.library.brown.edu/studio/item/bdr:792693/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mori M. Gene Expression Changes Associated with the Differentiation
of Mouse Embryonic Stem Cells to Primordial Germ Cell-Like
Cells. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792693/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
Irofuala, Chinedu.
Cardiac Differentiation Potential is Modulated by Genetic
Background: Implications for Personalized Medicine.
Degree: Biomedical Engineering, 2018, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:792817/
► Cardiovascular diseases are some of the most common and most lethal diseases in the world. On top of that, the only treatment option available that…
(more)
▼ Cardiovascular diseases are some of the most common
and most lethal diseases in the world. On top of that, the only
treatment option available that would allow for cardiovascular
function to be restored to healthy levels long-term is a heart
transplant. Not only are heart transplants difficult to come by –
the waiting list is almost double the number of hearts available
each year – but even this solution may require life-long
immunosuppressant and drug use. By instead using regenerative
medicine and tissue engineering, it is possible to create
innovative solutions using human induced pluripotent stem
cell
derived cardiomyocytes that may create a pathway towards restoring
functionality to damaged hearts. Furthermore, these cardiomyocytes
can be used to perform extensive cardiotoxicity testing in human
cells, providing a more accurate and appropriate medium for
assessing the implications of new drugs heading to market, as well
as how a patient’s heart may react to a prescribed drug regimen.
Currently, one of the largest barriers to these fields of research
is the large number of pure cardiomyocytes that must be produced to
meet the demands of academia and industry. This is the motivation
behind the work presented here, which aims to optimize the
cardiomyocyte
differentiation process of the GiPSC, NCRM-5, and
WTC-11 human induced pluripotent stem
cell lines by altering
seeding density and Chiron concentration. It is shown that high
seeding density (137,000 cells per well) and low Chiron (3 µM)
concentration produce the highest purity cardiomyocytes regardless
of lineage, but that the level of purity is dictated by the genetic
background of the
cell type. As tissue engineering and regenerative
medicine move closer to personalized medicine approaches, it
becomes increasingly important to understand the implications of
genetics in determining the
differentiation potential of a
patient’s own induced pluripotent stem cells for therapy and
treatment purposes.
Advisors/Committee Members: Coulombe, Kareen (Advisor), Shukla, Anita (Reader), Dawson, Michelle (Reader).
Subjects/Keywords: Cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Irofuala, C. (2018). Cardiac Differentiation Potential is Modulated by Genetic
Background: Implications for Personalized Medicine. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792817/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Irofuala, Chinedu. “Cardiac Differentiation Potential is Modulated by Genetic
Background: Implications for Personalized Medicine.” 2018. Thesis, Brown University. Accessed April 18, 2021.
https://repository.library.brown.edu/studio/item/bdr:792817/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Irofuala, Chinedu. “Cardiac Differentiation Potential is Modulated by Genetic
Background: Implications for Personalized Medicine.” 2018. Web. 18 Apr 2021.
Vancouver:
Irofuala C. Cardiac Differentiation Potential is Modulated by Genetic
Background: Implications for Personalized Medicine. [Internet] [Thesis]. Brown University; 2018. [cited 2021 Apr 18].
Available from: https://repository.library.brown.edu/studio/item/bdr:792817/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Irofuala C. Cardiac Differentiation Potential is Modulated by Genetic
Background: Implications for Personalized Medicine. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792817/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Saskatchewan
3.
Rudulier, Christopher.
CD4+ T cell interactions through CD28/B7 molecules affects their Th1/Th2 phenotype.
Degree: 2011, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2011-12-282
► The Th1/Th2 phenotype of the immune response generated against a pathogen or disease can have a profound impact upon the survival of the host. Thus,…
(more)
▼ The Th1/Th2 phenotype of the immune response generated against a pathogen or disease can have a profound impact upon the survival of the host. Thus, a complete and accurate model for the generation of Th1 versus Th2 cells is critical for the development of effective immunotherapeutic strategies aimed against diseases including cancer and tuberculosis.
Numerous factors are considered to be important in the differential development of Th1 and Th2 cells. These include the strength of stimulation through the T
cell receptor, the cytokine environment, the dose of antigen, and the types of co-stimulatory molecules expressed by the antigen-presenting cells presenting the T cell’s cognate antigen. A common thread found in the majority of studies on the generation of Th1 and Th2 cells is that naïve CD4+ T cells are thought to be passive players in this process. In contrast, our laboratory has demonstrated, in a number of in vivo systems, that the density of the responding naïve CD4+ T cells, that is to say the frequency of responding CD4+ T cells in an animal, has a significant impact on their Th1/Th2 phenotype. In particular, our laboratory has reported that in response to antigen, a low density of responding naïve CD4+ T cells give rise to Th1 cells while a high density of responding CD4+ T cells give rise to Th2 cells. Although we have made these observations, we had not, prior to the work described herein, elucidated the mechanism(s) surrounding this phenomenon. This was the impetus for the experiments detailed in this thesis.
In order to investigate the mechanism(s) mediating the effect that the density of responding CD4+ T cells has on the Th1/Th2 phenotype, we have developed an in vitro system that employs a physiological number of naïve TCR transgenic CD4+ T cells (DO11.10) specific for the OVA323-339 peptide in the context of I-Ad. My observations in this in vitro system mirror
previous in vivo observation in that a low frequency of naïve CD4+ DO11.10 T cells cultured with syngeneic APC and the OVA323-339 peptide, acquire a Th1 phenotype while naïve CD4+ DO11.10 T cells cultured at high frequencies, under identical conditions, acquire a Th2 phenotype.
By controlling the type of APC in our culture system, we have ascertained that the effect of CD4+ T
cell density on the differential generation of Th1 and Th2 cells is mediated by B cells, but not dendritic cells. We also found that the addition of an agonistic anti-CD28 antibody skews the phenotype of T cells cultured at low densities towards the Th2 pole while partially blocking of CD80 and CD86, through the addition of CTLA4-Ig to high density T
cell cultures, resulted in the development of Th1 cells. In contrast, modulating the interactions between CD40/CD40L and OX40/OX40L, two co-stimulatory molecule/ligand pairs known to affect Th1/Th2
differentiation, through the addition of agonistic and antagonistic antibodies in high or low density CD4+ T
cell cultures did not alter the Th1/Th2 phenotype of the newly activated DO11.10 T cells. Thus, it appears…
Advisors/Committee Members: Bretscher, Peter, Havele, Calliopi, Gerdts, Volker, Bull, Harold, Goldie, Hughes.
Subjects/Keywords: CD4 T cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rudulier, C. (2011). CD4+ T cell interactions through CD28/B7 molecules affects their Th1/Th2 phenotype. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2011-12-282
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rudulier, Christopher. “CD4+ T cell interactions through CD28/B7 molecules affects their Th1/Th2 phenotype.” 2011. Thesis, University of Saskatchewan. Accessed April 18, 2021.
http://hdl.handle.net/10388/ETD-2011-12-282.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rudulier, Christopher. “CD4+ T cell interactions through CD28/B7 molecules affects their Th1/Th2 phenotype.” 2011. Web. 18 Apr 2021.
Vancouver:
Rudulier C. CD4+ T cell interactions through CD28/B7 molecules affects their Th1/Th2 phenotype. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10388/ETD-2011-12-282.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rudulier C. CD4+ T cell interactions through CD28/B7 molecules affects their Th1/Th2 phenotype. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/ETD-2011-12-282
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Ottawa
4.
Kocharyan, Avetik.
Derivation and Characterization of Pax7 Positive Skeletal Muscle Precursor Cells from Control and HGPS-derived induced Pluripotent Stem Cells
.
Degree: 2018, University of Ottawa
URL: http://hdl.handle.net/10393/37517
► Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare genetic disorder associated with premature aging in various tissues and organs of the afflicted individuals, including accelerated skeletal…
(more)
▼ Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare genetic disorder associated with premature aging in various tissues and organs of the afflicted individuals, including accelerated skeletal muscle atrophy. Classical HGPS manifests due to single-base substitution in the LAMNA gene which encodes Lamin A/C proteins. As a result of the mutation, a truncated form of Lamin (known as Progerin) is produced which undergoes persistent farnesylation during post-translational modification. Accumulation of Progerin in the nucleus has been linked to various cellular abnormalities including abnormal nuclear morphologies and altered chromatin organization, among others. However, the exact molecular mechanisms leading to skeletal muscle atrophy have not yet been elucidated. In this study, the iPSC approach was implemented in order to study the skeletal muscle phenotype of HGPS by generating and characterizing a population of Pax7 positive skeletal muscle precursor cells (SMPs).
During the course of this project, we have demonstrated the need for excessive optimization of the previously developed directed differentiation protocol for successful application on induced Pluripotent Stem Cells. Furthermore, we have successfully modified the protocol to allow for a more rapid expansion of the SMPs through regular passaging of the myogenic cells starting on day 20 of differentiation. Additionally, this new method produced more uniform distribution of the myogenic cells and allowed for successful freezing/thawing of the myogenic cells.
When compared to the controls, the HGPS-derived SMPs did not appear to be defective in formation, proliferation or differentiation. Abnormal nuclear morphology and DNA damage, documented in HGPS fibroblasts and vascular smooth muscle cells, were not detected the in myogenic cells. Furthermore, we were not able to detect Progerin protein accumulation in the generated myogenic cultures, offering an explanation for the absence of these phenotypes in the skeletal muscle system.
Subjects/Keywords: Muscle;
Stem cell;
Progeria;
Differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kocharyan, A. (2018). Derivation and Characterization of Pax7 Positive Skeletal Muscle Precursor Cells from Control and HGPS-derived induced Pluripotent Stem Cells
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/37517
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kocharyan, Avetik. “Derivation and Characterization of Pax7 Positive Skeletal Muscle Precursor Cells from Control and HGPS-derived induced Pluripotent Stem Cells
.” 2018. Thesis, University of Ottawa. Accessed April 18, 2021.
http://hdl.handle.net/10393/37517.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kocharyan, Avetik. “Derivation and Characterization of Pax7 Positive Skeletal Muscle Precursor Cells from Control and HGPS-derived induced Pluripotent Stem Cells
.” 2018. Web. 18 Apr 2021.
Vancouver:
Kocharyan A. Derivation and Characterization of Pax7 Positive Skeletal Muscle Precursor Cells from Control and HGPS-derived induced Pluripotent Stem Cells
. [Internet] [Thesis]. University of Ottawa; 2018. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10393/37517.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kocharyan A. Derivation and Characterization of Pax7 Positive Skeletal Muscle Precursor Cells from Control and HGPS-derived induced Pluripotent Stem Cells
. [Thesis]. University of Ottawa; 2018. Available from: http://hdl.handle.net/10393/37517
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas Southwestern Medical Center
5.
Shields, Benjamin Baker.
Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer.
Degree: 2013, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1589
► Recent undertakings to identify the genetic lesions associated with ovarian cancer have noted the striking diversity of mutations occurring in this disease. This genetic diversity…
(more)
▼ Recent undertakings to identify the genetic lesions associated with ovarian cancer have noted the striking diversity of mutations occurring in this disease. This genetic diversity has complicated the search for novel therapies. However, recent data has suggested that one commonality of ovarian tumors might be ablation of miRNA biogenesis. Here I conducted a broad-scale gain-of-function microRNA (miRNA) screen in 16 ovarian cancer
cell lines to annotate the functional landscape present in such a chaotic genetic background. miRNAs function as multigenic perturbations allowing for interrogation of maximal gene space with few experiments. This screen identified multiple miRNAs reducing
cell viability with the majority of hits being toxic in only one or two lines screened. This surprising finding reflected the commonality of altered miRNA function in ovarian tumors while also suggesting that specifics of this alteration in function are unique to each tumor. To investigate more public vulnerabilities, I focused mechanistic studies on miRNAs displaying penetrance in greater than 5
cell lines. miR-517a reduced
cell viability in over 30% of the panel and also reduced tumor burden in vivo. Functional analysis of the predicted targets of miR-517a revealed that expression of this miRNA reduced protein levels of ARCN1, a member of the coatamer complex, and that knockdown of ARCN1 reduced
cell viability similar to miR-517a. Another penetrant miRNA, miR-124a, reduced
cell viability in 37.5% of the panel and functional analysis of this miRNA revealed it promoted a
cell differentiation program. Analysis of predicted targets revealed that expression of miR-124a reduced expression the homeodomain transcription factor SIX4, resulting in increased signaling along the tumor suppressive AMPK pathway and epithelial
differentiation. Furthermore, SIX4 displayed increased expression in ovarian tumors and depletion of SIX4 expression reduced tumor
cell viability in vitro and in vivo. Therefore, SIX4 overexpression might function to deflect
cell differentiation in tumors. Thus, the common loss of miRNA function observed in ovarian tumors might serve to maintain an undifferentiated state, and engagement of
cell fate determination programs via re-expression of miRNAs can result in catastrophic consequences for cancer
cell viability.
Advisors/Committee Members: Castrillon, Diego H., White, Michael A., Altschuler, Steven J., Brekken, Rolf A..
Subjects/Keywords: Cell Differentiation; MicroRNAs; Ovarian Neoplasms
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shields, B. B. (2013). Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1589
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shields, Benjamin Baker. “Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed April 18, 2021.
http://hdl.handle.net/2152.5/1589.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shields, Benjamin Baker. “Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer.” 2013. Web. 18 Apr 2021.
Vancouver:
Shields BB. Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/2152.5/1589.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shields BB. Mapping the Landscape of Acquired Vulnerabilities in Ovarian Cancer. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1589
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Central Connecticut State University
6.
Banda, Erin.
Identification of the first differentiation event involved in human embryonic stem cell-derived neural lineage specification.
Degree: Department of Biomolecular Sciences, 2010, Central Connecticut State University
URL: http://content.library.ccsu.edu/u?/ccsutheses,1569
► The ability of embryonic stem cells to give rise to all cells comprising the three germ layers – endoderm, mesoderm and ectoderm – highlights a…
(more)
▼ The ability of embryonic stem cells to give rise to all cells comprising the three germ layers – endoderm, mesoderm and ectoderm – highlights a powerful in vivo system for the study of early embryonic development. Human embryonic stem
cell (hESC) derived- neural stem cells (NSCs) provide a means for understanding development of the mammalian nervous system as well as neurodegenerative diseases associated with atypical development and disease pathology of the nervous system. Here I demonstrate identification of the earliest neural stem
cell gene expressed during in vitro NSC specification. In this study, Musashi1 (Msi1), a member of the Notch signaling pathway, has interestingly been identified as the earliest NSC marker expressed during monolayer
differentiation of hESCs towards NSC fates. Notch signaling is well classified in its role for maintenance of NSC populations in vivo, as well as in vitro, but this early Msi1 expression suggests that Notch signaling may have a role in specification of early neurectodermal cells. The identification of the earliest NSC gene in vivo allows the possibility of the development of a hESC-derived NSC reporter line, capable of highlighting cells as they become allocated to the neural lineage. Additionally, purification of these early NSCs allows for the study and transplantation of purified NSC populations, without contaminating ESCs or other proliferative
cell types capable of forming teratocarcinomas.
Advisors/Committee Members: Mulrooney, James P;.
Subjects/Keywords: Cell differentiation; Embryonic stem cells
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Banda, E. (2010). Identification of the first differentiation event involved in human embryonic stem cell-derived neural lineage specification. (Thesis). Central Connecticut State University. Retrieved from http://content.library.ccsu.edu/u?/ccsutheses,1569
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Banda, Erin. “Identification of the first differentiation event involved in human embryonic stem cell-derived neural lineage specification.” 2010. Thesis, Central Connecticut State University. Accessed April 18, 2021.
http://content.library.ccsu.edu/u?/ccsutheses,1569.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Banda, Erin. “Identification of the first differentiation event involved in human embryonic stem cell-derived neural lineage specification.” 2010. Web. 18 Apr 2021.
Vancouver:
Banda E. Identification of the first differentiation event involved in human embryonic stem cell-derived neural lineage specification. [Internet] [Thesis]. Central Connecticut State University; 2010. [cited 2021 Apr 18].
Available from: http://content.library.ccsu.edu/u?/ccsutheses,1569.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Banda E. Identification of the first differentiation event involved in human embryonic stem cell-derived neural lineage specification. [Thesis]. Central Connecticut State University; 2010. Available from: http://content.library.ccsu.edu/u?/ccsutheses,1569
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Harvard University
7.
Weinreb, Caleb.
Gene Expression Dynamics in Single Cells.
Degree: PhD, 2019, Harvard University
URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42013156
► Dynamic regulation of gene expression is central to fate choice and differentiation. Genes drive changes in cell state and also serve as markers of mature…
(more)
▼ Dynamic regulation of gene expression is central to fate choice and differentiation. Genes drive changes in cell state and also serve as markers of mature cell types and immature intermediates. Advances in single-cell RNA-seq (scSeq) have made it possible measure the expression of thousands of genes in tens of thousands of cells in a single experiment. Single-cell RNA sequencing (scSeq) opens up a new terrain in the study of differentiation and fate choice, but it has an important limitation: existing methods kill cells in the process of measurement, and thus prevent following a cell’s state over time. The inability to track cells prevents a full understanding of the dynamic events that accompany differentiation, or how variation in the state of progenitor cells predisposes their sub- sequent fate choices. These limitations are particularly evident in hematopoiesis – the process of steady-state blood production in bone marrow. scSeq is increasingly used to characterize the heterogeneity of hematopoietic stem and progenitor cells, but the functional consequences of this heterogeneity have been difficult to ascertain. Here, several methods are described for inferring single-cell gene expression dynamics. Chapter 1 presents a visualization method called SPRING that facilitates human inference of dynamics. A more direct approach to infer dynamics from high dimensional data is described in chapter 2 based on the principle of population balance. This analysis also highlights several properties of gene expression dynamics that cannot be inferred from a single-cell snapshot, including: whether unmeasured variables contribute significantly to fate choice; the degree to which cells in the same gene expression state also have the same velocity in their state; and where fate commitment occurs in gene expression space. Chapter 3 describes an experimental approach for tracking cell state over time that involves clonally marking cells, letting them divide, measuring one sister right away, and measuring the other sister later on. Applied to hematopoiesis, this method partially addresses the questions raised in Chapter 2, demonstrating the existence of hidden variables during differentiation and mapping fate probability across the gene expression landscape. It also opens up new directions for perturbational analysis of hematopoietic fate choice, and may pave the way for linking cell state and fate in other systems.
Systems Biology
Advisors/Committee Members: Mitchison, Timothy (advisor), Singer, Meromit (committee member), Camargo, Fernando (committee member).
Subjects/Keywords: Single-cell; dynamic inference; differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Weinreb, C. (2019). Gene Expression Dynamics in Single Cells. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:42013156
Chicago Manual of Style (16th Edition):
Weinreb, Caleb. “Gene Expression Dynamics in Single Cells.” 2019. Doctoral Dissertation, Harvard University. Accessed April 18, 2021.
http://nrs.harvard.edu/urn-3:HUL.InstRepos:42013156.
MLA Handbook (7th Edition):
Weinreb, Caleb. “Gene Expression Dynamics in Single Cells.” 2019. Web. 18 Apr 2021.
Vancouver:
Weinreb C. Gene Expression Dynamics in Single Cells. [Internet] [Doctoral dissertation]. Harvard University; 2019. [cited 2021 Apr 18].
Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42013156.
Council of Science Editors:
Weinreb C. Gene Expression Dynamics in Single Cells. [Doctoral Dissertation]. Harvard University; 2019. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42013156
California State University – Sacramento
8.
Torres, Christian I.
Development of induced pluripotent stem cell-derived self-organizing renal organoids.
Degree: MS, Biological Sciences (Stem Cell, 2019, California State University – Sacramento
URL: http://hdl.handle.net/10211.3/212754
► Human pluripotent stem cells have the potential to serve as a model of organ development and disease when grown in a three-dimensional (3D) structure. Human…
(more)
▼ Human pluripotent stem cells have the potential to serve as a model of organ development and disease when grown in a three-dimensional (3D) structure. Human kidney development results from the reciprocal inductive interactions involving the metanephric mesenchyme (future excretory component of the kidney) and the ureteric bud (future collecting system). One of the challenges in creating kidney organoids is recapitulating the complex interactions between these primordial structures. Prior studies have shown methods to achieve either metanephric mesenchyme or ureteric bud lineage commitment from human induced pluripotent stem cells (hiPSC); however, a single protocol to achieve both lineages simultaneously is in need of further assessment. The goals of these studies were to expand a well-characterized bank of hiPSC, and to test a self-organizing
differentiation protocol to determine if commitment to metanephric mesenchyme and ureteric bud precursors could be obtained in a combined culture system using either transwells or a 3D culture system. Studies focused on evaluating directed
differentiation of hiPSC toward metanephric and ureteric bud lineages and recapitulating kidney development. The collection of cells was performed at select time points for gene expression analysis using quantitative PCR (qPCR) and at study endpoint for histologic analysis and immunohistochemistry. hiPSC were expanded and cryopreserved with characterization demonstrating pluripotency (endoderm, mesoderm, and ectoderm) by qPCR. When hiPSC were directed toward renal lineage
differentiation, gene expression studies showed upregulation of the mesodermal marker brachyury, and markers of anterior and posterior intermediate mesoderm suggesting
differentiation toward ureteric bud and metanephric mesenchyme phenotypes, respectively. Differentiated hiPSC formed tubules resembling early kidney development under both culture conditions. Analysis of renal developmental markers by immunohistochemistry showed similar expression patterns. The results of these studies support a
differentiation protocol that recapitulates early stages of renal development.
Advisors/Committee Members: Mulligan, Kimberly.
Subjects/Keywords: Directed differentiation; Stem cell; Organoids
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Torres, C. I. (2019). Development of induced pluripotent stem cell-derived self-organizing renal organoids. (Masters Thesis). California State University – Sacramento. Retrieved from http://hdl.handle.net/10211.3/212754
Chicago Manual of Style (16th Edition):
Torres, Christian I. “Development of induced pluripotent stem cell-derived self-organizing renal organoids.” 2019. Masters Thesis, California State University – Sacramento. Accessed April 18, 2021.
http://hdl.handle.net/10211.3/212754.
MLA Handbook (7th Edition):
Torres, Christian I. “Development of induced pluripotent stem cell-derived self-organizing renal organoids.” 2019. Web. 18 Apr 2021.
Vancouver:
Torres CI. Development of induced pluripotent stem cell-derived self-organizing renal organoids. [Internet] [Masters thesis]. California State University – Sacramento; 2019. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10211.3/212754.
Council of Science Editors:
Torres CI. Development of induced pluripotent stem cell-derived self-organizing renal organoids. [Masters Thesis]. California State University – Sacramento; 2019. Available from: http://hdl.handle.net/10211.3/212754
Rutgers University
9.
Ambegaonkar, Abhijit Ashok, 1985-.
Regulation of planar cell polarity by the Dachsous-Fat pathway.
Degree: PhD, Cell and Developmental Biology, 2015, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49140/
► Planar cell polarity is the polarization of cells within the plane of a tissue. The Dachsous-Fat pathway plays a key role in the regulation of…
(more)
▼ Planar cell polarity is the polarization of cells within the plane of a tissue. The Dachsous-Fat pathway plays a key role in the regulation of planar cell polarity and growth during the development of an organism. The transmembrane cadherins Fat and Dachsous from neighboring cells interact heterophilically and regulate the localization of the unconventional myosin Dachs in a cell. Four-jointed, a Golgi-localized kinase modulates the binding between Dachsous and Fat. Dachsous and Four-jointed are expressed in tissue-wide gradients, which influence Fat activity and direct the polarized membrane localization of Dachs. We demonstrate that differential expression of Dachsous or Four-jointed can modulate Dachs polarization at a distance in wing discs. This indicates that Dachsous and Four-jointed gradients can be measured over long range in a tissue through propagation of polarity. We also show that Dachsous and Fat are partially polarized along the endogenous Dachsous and Four-jointed gradients, providing a mechanism for propagation of polarity. Through directed membrane targeting of Dachs, we show that membrane localization of Dachs influences both planar cell polarity and the Hippo signaling pathway. These studies help in understanding the mechanisms involved in establishment and maintenance of planar cell polarity. The Frizzled pathway is another key pathway that regulates planar cell polarity, but its relationship with the Dachsous-Fat pathway was unclear. We demonstrate that Dachs and Dachsous can independently interact with a Frizzled pathway component, Spiny-legs, and direct its localization in vivo. Thus, the Dachsous-Fat pathway provides directional input to the Frizzled pathway by influencing the localization of Spiny-legs. These studies help in understanding how planar cell polarity is regulated in various tissues through coordination between Dachsous-Fat and Frizzled pathways. These studies also reveal that Spiny-legs and its isoform Prickle can respond to distinct planar cell polarity signals and allow the cells to compare and choose between these competing signals to direct polarity robustly in one direction. Thus, our results identify a mechanism for propagation of planar cell polarity through the tissue, establish the significance of Dachs membrane localization in Dachsous-Fat signaling and identify a molecular mechanism for crosstalk between the two planar cell polarity pathways.
Advisors/Committee Members: Grant, Barth (chair), Irvine, Kenneth (internal member), Steward, Ruth (internal member), Soto, Martha (outside member).
Subjects/Keywords: Polarity (Biology); Cell differentiation; Drosophia
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ambegaonkar, Abhijit Ashok, 1. (2015). Regulation of planar cell polarity by the Dachsous-Fat pathway. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49140/
Chicago Manual of Style (16th Edition):
Ambegaonkar, Abhijit Ashok, 1985-. “Regulation of planar cell polarity by the Dachsous-Fat pathway.” 2015. Doctoral Dissertation, Rutgers University. Accessed April 18, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/49140/.
MLA Handbook (7th Edition):
Ambegaonkar, Abhijit Ashok, 1985-. “Regulation of planar cell polarity by the Dachsous-Fat pathway.” 2015. Web. 18 Apr 2021.
Vancouver:
Ambegaonkar, Abhijit Ashok 1. Regulation of planar cell polarity by the Dachsous-Fat pathway. [Internet] [Doctoral dissertation]. Rutgers University; 2015. [cited 2021 Apr 18].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49140/.
Council of Science Editors:
Ambegaonkar, Abhijit Ashok 1. Regulation of planar cell polarity by the Dachsous-Fat pathway. [Doctoral Dissertation]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49140/
King Abdullah University of Science and Technology
10.
Noutsi, Bakiza Kamal.
Correlation between membrane fluidity cellular development and stem cell differentiation.
Degree: Biological and Environmental Sciences and Engineering (BESE) Division, 2016, King Abdullah University of Science and Technology
URL: http://hdl.handle.net/10754/621954
► Cell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During…
(more)
▼ Cell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal
differentiation,
cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various
cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different
cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during
differentiation. This study sheds light on the involvement of membrane fluidity during neuronal
differentiation and development of other
cell lines.
Advisors/Committee Members: Chaieb, Saharoui (advisor), Magistretti, Pierre J. (committee member), Khashab, Niveen M. (committee member), Gratton, Enrico (committee member).
Subjects/Keywords: Neuron; development; stem cell; Differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Noutsi, B. K. (2016). Correlation between membrane fluidity cellular development and stem cell differentiation. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/621954
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Noutsi, Bakiza Kamal. “Correlation between membrane fluidity cellular development and stem cell differentiation.” 2016. Thesis, King Abdullah University of Science and Technology. Accessed April 18, 2021.
http://hdl.handle.net/10754/621954.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Noutsi, Bakiza Kamal. “Correlation between membrane fluidity cellular development and stem cell differentiation.” 2016. Web. 18 Apr 2021.
Vancouver:
Noutsi BK. Correlation between membrane fluidity cellular development and stem cell differentiation. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2016. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10754/621954.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Noutsi BK. Correlation between membrane fluidity cellular development and stem cell differentiation. [Thesis]. King Abdullah University of Science and Technology; 2016. Available from: http://hdl.handle.net/10754/621954
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
11.
Lei, Fengyang.
Development of a T cell based cancer immunotherapy by using the induced pluripotent stem cell.
Degree: 2013, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/18747
► Cancer is one of the leading health issues that has caused tremendous impacts. Conquering cancer is imminent and finding more potent treatments to cancer is…
(more)
▼ Cancer is one of the leading health issues that has caused tremendous impacts. Conquering cancer is imminent and finding more potent treatments to cancer is also urgent. In searching for novel anti-cancer regimens, recently, a new concept of cancer immunotherapy has been proposed. It is found that by using adoptive T
cell transfer (ACT)-based immunotherapy, tumor regression can be achieved. However, a major problem of this strategy is the shortage of T cells for transfer. Previous work has shown that stem cells are good candidates for generating T
cell for therapeutic purposes; however, there are many hurdles existed. The recent discovery of the induced pluripotent stem (iPS)
cell technology hints that iPS cells are potential substitutes of natural stem cells for generating T cells being used in cancer immunotherapy.
The hypothesis of my research is iPS
cell is identical to other stem cells in the context of T lineage
differentiation, and furthermore, iPS
cell can be engineered and induced into antigen specific T
cell to bolster the tumor immune surveillance.
In the first study, it is observed iPS cells are able to differentiate into conventional T cells after in vitro Notch signaling pathway ligand stimulation. In vitro developed iPS cells express general T
cell markers, respond to costimulatory signal activation and reconstitute the T
cell pools of the recipient lymphopenic mice. Following this, a second work showed antigen-specific T cells are induced from iPS cells through the T
cell receptor (TCR) signal stimulation. In short, genetically modifying iPS cells with antigen-specific TCR can promote the development of corresponding T cells in vivo and developed T cells are functional in terms of responding to antigen stimulation and managing tumor growth. Further analyses suggested that both Notch and TCR signals may be involved, in a synergistic manner, in inducing iPS cells to develop into T cells; and probably, this process is mediated by the transforming growth factor (TGF)-β signaling pathway.
These studies have initially supported our hypothesis however both extensive and intensive studies are still needed to further test our central hypothesis. In conclusion, my dissertation study served as the first series of proof-of-concept work to investigate the feasibility of an iPS
cell-based, personalized cancer immunotherapy.
Advisors/Committee Members: Jianxun Song, Dissertation Advisor/Co-Advisor, Jianxun Song, Committee Chair/Co-Chair, Neil David Christensen, Committee Member, Todd Schell, Committee Member, John Warren Wills, Committee Member, Jiyue Zhu, Special Member.
Subjects/Keywords: stem cell; T cell; differentiation; immunotherapy
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lei, F. (2013). Development of a T cell based cancer immunotherapy by using the induced pluripotent stem cell. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/18747
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lei, Fengyang. “Development of a T cell based cancer immunotherapy by using the induced pluripotent stem cell.” 2013. Thesis, Penn State University. Accessed April 18, 2021.
https://submit-etda.libraries.psu.edu/catalog/18747.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lei, Fengyang. “Development of a T cell based cancer immunotherapy by using the induced pluripotent stem cell.” 2013. Web. 18 Apr 2021.
Vancouver:
Lei F. Development of a T cell based cancer immunotherapy by using the induced pluripotent stem cell. [Internet] [Thesis]. Penn State University; 2013. [cited 2021 Apr 18].
Available from: https://submit-etda.libraries.psu.edu/catalog/18747.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lei F. Development of a T cell based cancer immunotherapy by using the induced pluripotent stem cell. [Thesis]. Penn State University; 2013. Available from: https://submit-etda.libraries.psu.edu/catalog/18747
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Oklahoma
12.
Zhou, Ningyun.
Bacteriophage-based biomaterials for manipulating derivation and differentiation of human induced pluripotent stem cells.
Degree: PhD, 2020, University of Oklahoma
URL: http://hdl.handle.net/11244/324169
► Induced pluripotent stem cells (iPSCs), which are derived from somatic cells, can differentiate into any cell type. They are promising tools in medical applications including…
(more)
▼ Induced pluripotent stem cells (iPSCs), which are derived from somatic cells, can differentiate into any
cell type. They are promising tools in medical applications including regenerative medicine, personalized
cell therapy, disease modeling, and drug discovery. The current stem
cell research faces at least the following two major challenges: how to improve the reprogramming efficiency in iPSCs derivation; and how to control the
differentiation of stem cells into certain
cell types. The works in this dissertation attempt to find solutions to tackle the above two challenges.
To enhance the reprogramming efficiency of somatic cells into iPSCs, human dermal fibroblasts (HDFs)-internalizing peptides were selected using Phage Display Peptide Library. After the selection, 3 HDF-binding peptides with high occurrences were selected for further screening. Finally, the HDF-binding peptide with the strongest affinity and high specificity was chemically conjugated to the surface of a nanoparticle plasmid carrier to improve the endocytosis efficiency and further help with the reprogramming process.
To induce directional
differentiation of iPSCs or iPSC-derived stem cells, a novel 2D virus-based substrate with unique self-assembled hierarchical nano- and micro-topographies was developed. This substrate can direct the bidirectional
differentiation of iPSC-derived neural progenitor cells (NPCs) into neurons and astrocytes without the use of costly growth factors, which also provide a new approach for studying the interaction between neurons and astrocytes.
Advisors/Committee Members: Rajan, Rakhi (advisor), Hewes, Randall (committee member), Wu, Si (committee member), Burgett, Anthony (committee member).
Subjects/Keywords: Biomaterials; Cell reprogramming; Stem cell differentiation; iPSC
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhou, N. (2020). Bacteriophage-based biomaterials for manipulating derivation and differentiation of human induced pluripotent stem cells. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/324169
Chicago Manual of Style (16th Edition):
Zhou, Ningyun. “Bacteriophage-based biomaterials for manipulating derivation and differentiation of human induced pluripotent stem cells.” 2020. Doctoral Dissertation, University of Oklahoma. Accessed April 18, 2021.
http://hdl.handle.net/11244/324169.
MLA Handbook (7th Edition):
Zhou, Ningyun. “Bacteriophage-based biomaterials for manipulating derivation and differentiation of human induced pluripotent stem cells.” 2020. Web. 18 Apr 2021.
Vancouver:
Zhou N. Bacteriophage-based biomaterials for manipulating derivation and differentiation of human induced pluripotent stem cells. [Internet] [Doctoral dissertation]. University of Oklahoma; 2020. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/11244/324169.
Council of Science Editors:
Zhou N. Bacteriophage-based biomaterials for manipulating derivation and differentiation of human induced pluripotent stem cells. [Doctoral Dissertation]. University of Oklahoma; 2020. Available from: http://hdl.handle.net/11244/324169
Swedish University of Agricultural Sciences
13.
Johnsson, Christoffer.
Elucidating the phytohormonal control of xylem development.
Degree: 2018, Swedish University of Agricultural Sciences
URL: https://pub.epsilon.slu.se/15386/
► Secondary xylem, commonly known as wood, has had a major impact on both our planet and civilization, its uses so ubiquitous that it is often…
(more)
▼ Secondary xylem, commonly known as wood, has had a major impact on both our planet and civilization, its uses so ubiquitous that it is often taken for granted in daily life.
New challenges imposed by growing population and changing climate have led academia and industries to search for ways of altering wood characteristics to better suit specific purposes. Achieving this by genetic engineering requires the ability to alter gene expression in specific cell types, while avoiding alterations in others. This calls for an in depth understanding of the factors directing the differentiation and development of the cell types constituting secondary xylem.
Studies detailed in this thesis exhibit my attempts to link the signals of the phytohormones auxin and gibberellic acid to the formation of fibres, and their deposition of thick secondary cell walls. Using high resolution RNA-sequencing data gathered across developing Populus wood, I was able to illustrate that the expression profiles of various wood-associated transcription factors are distinct, in relation to their functions. Furthermore, I investigated the response of gene expression and tissue anatomy to treatment by auxin and gibberellin and found that auxin down-regulates transcription factors previously identified as master regulators of fibre secondary cell wall formation, as well as impede secondary wall development on a tissue level. At the same time, gibberellic acid appears to have a general enhancing effect on the expression of the same genes, and is better able to rescue the tissue phenotype of decapitated Populus plants.
I also highlight a role for gibberellic acid in the differentiation of fibre cells through DELLA regulated interaction of Class I KNOX transcription factors with proteins of the NUCLEAR FACTOR Y family.
These findings suggest that an integration of auxin and gibberellin signals, acting through multiple pathways, is responsible for the correct spatiotemporal differentiation and development of the secondary xylem.
Subjects/Keywords: xylem; auxins; gibberellins; cell differentiation; cell walls; Xylem; Auxin; Gibberellin; differentiation; secondary cell wall
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Johnsson, C. (2018). Elucidating the phytohormonal control of xylem development. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from https://pub.epsilon.slu.se/15386/
Chicago Manual of Style (16th Edition):
Johnsson, Christoffer. “Elucidating the phytohormonal control of xylem development.” 2018. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 18, 2021.
https://pub.epsilon.slu.se/15386/.
MLA Handbook (7th Edition):
Johnsson, Christoffer. “Elucidating the phytohormonal control of xylem development.” 2018. Web. 18 Apr 2021.
Vancouver:
Johnsson C. Elucidating the phytohormonal control of xylem development. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2018. [cited 2021 Apr 18].
Available from: https://pub.epsilon.slu.se/15386/.
Council of Science Editors:
Johnsson C. Elucidating the phytohormonal control of xylem development. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2018. Available from: https://pub.epsilon.slu.se/15386/
University of Cambridge
14.
Geti, Imbisaat.
Production of hepatocytes from human pluripotent stem cells for cell-based therapy.
Degree: PhD, 2019, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/291327
► Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow…
(more)
▼ Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow for large-scale production of hepatocytes necessary for cell-based therapy. Importantly, cell-based therapy involving the transplantation of hepatocytes-like cells (HLCs) represent a promising alternative to liver transplantation. However, there are two major limitations that must be overcome before hPSCs derived hepatocytes can go into clinical trials. Firstly, current protocols are not compatible with Good Manufacturing Practice (GMP) as they rely on culture media which are poorly defined and contain reagents of animal origin. Secondly, HLCs derived in vitro lack the full spectrum of adult hepatocyte functionalities and expressed foetal markers indicating that they are not equivalent to primary cells.
In this dissertation, I describe a method for generating a homogenous population of hepatocytes from hPSCs in conditions compatible with clinical applications. Furthermore, I show that the resulting GMP-like protocol is equally robust as research grade protocol to produce HLCs displaying key characteristics of their in vivo counterparts. Importantly, the efficacy of this new GMP protocol was validated in five different hPSCs lines, including three GMP grade human Embryonic Stem Cell lines. Finally, we have also characterized the resulting cells in vivo and demonstrated their safety after transplantation into immune deficient mice.
In parallel, I have optimised our protocol of differentiation by establishing a 5 steps method that closely follows liver development. For the development of this protocol, I have screened a large number of culture conditions and identified optimum time points for each step especially for hepatoblast and foetal hepatocytes stage.
Overall, the work presented in this dissertation has advanced the differentiation of hPSCs for cell-based therapy in two different ways. Firstly, it shows that production of HLCs in GMP like condition is feasible, safe and that the resulting cells could be useful for cell-based therapy in the context of liver diseases. Secondly, I have developed a new protocol with an optimised hepatoblast stage that will facilitate further clinical applications.
Subjects/Keywords: Hepatocytes; Cell based therapy; regenerative medicine; liver; liver differentiation; hepatocytes differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Geti, I. (2019). Production of hepatocytes from human pluripotent stem cells for cell-based therapy. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/291327
Chicago Manual of Style (16th Edition):
Geti, Imbisaat. “Production of hepatocytes from human pluripotent stem cells for cell-based therapy.” 2019. Doctoral Dissertation, University of Cambridge. Accessed April 18, 2021.
https://www.repository.cam.ac.uk/handle/1810/291327.
MLA Handbook (7th Edition):
Geti, Imbisaat. “Production of hepatocytes from human pluripotent stem cells for cell-based therapy.” 2019. Web. 18 Apr 2021.
Vancouver:
Geti I. Production of hepatocytes from human pluripotent stem cells for cell-based therapy. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Apr 18].
Available from: https://www.repository.cam.ac.uk/handle/1810/291327.
Council of Science Editors:
Geti I. Production of hepatocytes from human pluripotent stem cells for cell-based therapy. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/291327
University of Toronto
15.
Soleas, John.
Guided Self-assembly of Human-pluripotent-Stem-cell-derived-lung-progenitors into Simplified Developmentally Relevant Architecture.
Degree: PhD, 2019, University of Toronto
URL: http://hdl.handle.net/1807/97689
► Chemical and mechanical cues are known to guide the differentiation of pluripotent stem cells (PSCs) towards specific cell-types; a process known as directed differentiation. Typically,…
(more)
▼ Chemical and mechanical cues are known to guide the
differentiation of pluripotent stem cells (PSCs) towards specific
cell-types; a process known as directed
differentiation. Typically, directed
differentiation protocols to guide PSCs to specific
cell types have focused on chemical cues. For example, lung epithelial directed
differentiation protocols mimic the chemical cues observed in lung organogenesis. The products of current directed
differentiation protocols are typically unorganized cellular aggregates that are often limited by their inability to generate specific
cell types reliably. This suggests further improvements to these protocols are necessary for reliable
cell manufacturing. Introducing developmentally relevant mechanical signals offers a potentially novel strategy to improve the predictability and reduce the heterogeneity of current directed
differentiation protocols. To assess the feasibility of this concept we use polymeric substrates to guide the self-assembly of human PSC-derived lung progenitors into developmentally relevant sized tubes and assess the impact of defined architecture on
differentiation. Specifically, day 17 ventralized lung progenitors, dual positive for proximal and distal lung markers SOX2 and SOX9, respectively, were cultured in polymeric tubes with diameters of 100µm and allowed to self-assemble into cellular tubes. Cells cultured in tubes were observed to become primarily SOX9+ as compared to cells in flat cultures that remained dual positive. Cells from tube cultures were retrieved and exposed to proximal or distal airway inducing conditions. Our data show that cells from flat culture are capable of expressing proximal and distal markers under proximal and distal conditions, respectively. However, cells retrieved from tubes were able to express distal markers under distal conditions but not proximal markers under proximal conditions. Our results suggest that modulation of tension in the presence of canonical WNT signaling by culturing the dual positive cells in the 100µm tubes resulted in loss of SOX2 expression. Overall, this thesis suggests that the use of a bioinspired mechanical microenvironment to organize and control the
differentiation of developing lung epithelial populations during directed
differentiation is a tractable strategy to control cellular
differentiation in vitro.
Advisors/Committee Members: McGuigan, Alison P, Waddell, Thomas K, Biomedical Engineering.
Subjects/Keywords: Biophysical forces; Lung differentiation; Stem cell differentiation; Tissue Engineering; 0202
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Soleas, J. (2019). Guided Self-assembly of Human-pluripotent-Stem-cell-derived-lung-progenitors into Simplified Developmentally Relevant Architecture. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/97689
Chicago Manual of Style (16th Edition):
Soleas, John. “Guided Self-assembly of Human-pluripotent-Stem-cell-derived-lung-progenitors into Simplified Developmentally Relevant Architecture.” 2019. Doctoral Dissertation, University of Toronto. Accessed April 18, 2021.
http://hdl.handle.net/1807/97689.
MLA Handbook (7th Edition):
Soleas, John. “Guided Self-assembly of Human-pluripotent-Stem-cell-derived-lung-progenitors into Simplified Developmentally Relevant Architecture.” 2019. Web. 18 Apr 2021.
Vancouver:
Soleas J. Guided Self-assembly of Human-pluripotent-Stem-cell-derived-lung-progenitors into Simplified Developmentally Relevant Architecture. [Internet] [Doctoral dissertation]. University of Toronto; 2019. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/1807/97689.
Council of Science Editors:
Soleas J. Guided Self-assembly of Human-pluripotent-Stem-cell-derived-lung-progenitors into Simplified Developmentally Relevant Architecture. [Doctoral Dissertation]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/97689
University of Cambridge
16.
Geti, Imbisaat.
Production of hepatocytes from human pluripotent stem cells for cell-based therapy.
Degree: PhD, 2019, University of Cambridge
URL: https://doi.org/10.17863/CAM.38508
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774691
► Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow…
(more)
▼ Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow for large-scale production of hepatocytes necessary for cell-based therapy. Importantly, cell-based therapy involving the transplantation of hepatocytes-like cells (HLCs) represent a promising alternative to liver transplantation. However, there are two major limitations that must be overcome before hPSCs derived hepatocytes can go into clinical trials. Firstly, current protocols are not compatible with Good Manufacturing Practice (GMP) as they rely on culture media which are poorly defined and contain reagents of animal origin. Secondly, HLCs derived in vitro lack the full spectrum of adult hepatocyte functionalities and expressed foetal markers indicating that they are not equivalent to primary cells. In this dissertation, I describe a method for generating a homogenous population of hepatocytes from hPSCs in conditions compatible with clinical applications. Furthermore, I show that the resulting GMP-like protocol is equally robust as research grade protocol to produce HLCs displaying key characteristics of their in vivo counterparts. Importantly, the efficacy of this new GMP protocol was validated in five different hPSCs lines, including three GMP grade human Embryonic Stem Cell lines. Finally, we have also characterized the resulting cells in vivo and demonstrated their safety after transplantation into immune deficient mice. In parallel, I have optimised our protocol of differentiation by establishing a 5 steps method that closely follows liver development. For the development of this protocol, I have screened a large number of culture conditions and identified optimum time points for each step especially for hepatoblast and foetal hepatocytes stage. Overall, the work presented in this dissertation has advanced the differentiation of hPSCs for cell-based therapy in two different ways. Firstly, it shows that production of HLCs in GMP like condition is feasible, safe and that the resulting cells could be useful for cell-based therapy in the context of liver diseases. Secondly, I have developed a new protocol with an optimised hepatoblast stage that will facilitate further clinical applications.
Subjects/Keywords: Hepatocytes; Cell based therapy; regenerative medicine; liver; liver differentiation; hepatocytes differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Geti, I. (2019). Production of hepatocytes from human pluripotent stem cells for cell-based therapy. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.38508 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774691
Chicago Manual of Style (16th Edition):
Geti, Imbisaat. “Production of hepatocytes from human pluripotent stem cells for cell-based therapy.” 2019. Doctoral Dissertation, University of Cambridge. Accessed April 18, 2021.
https://doi.org/10.17863/CAM.38508 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774691.
MLA Handbook (7th Edition):
Geti, Imbisaat. “Production of hepatocytes from human pluripotent stem cells for cell-based therapy.” 2019. Web. 18 Apr 2021.
Vancouver:
Geti I. Production of hepatocytes from human pluripotent stem cells for cell-based therapy. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Apr 18].
Available from: https://doi.org/10.17863/CAM.38508 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774691.
Council of Science Editors:
Geti I. Production of hepatocytes from human pluripotent stem cells for cell-based therapy. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://doi.org/10.17863/CAM.38508 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.774691
University of Sydney
17.
Wazin, Fatima.
Spred: a negative regulator of cellular processes involved in lens and eye development
.
Degree: 2020, University of Sydney
URL: http://hdl.handle.net/2123/22989
► The transparent and refractive properties of the lens are extremely dependent on the its precise cellular structure, with constant regulation of cell behaviour throughout life.…
(more)
▼ The transparent and refractive properties of the lens are extremely dependent on the its precise cellular structure, with constant regulation of cell behaviour throughout life. Cell signalling pathways play a crucial role in regulating cellular processes in both mammalian growth and development, and are in turn frequently regulated by inhibitory molecules, including the Spred family, to modulate and attenuate the impact of growth factor stimulation. Due to Spred’s strong expression in lens and its ability to negatively regulate growth factor-induced ERK/MAPK pathways that are essential for many biological events, we hypothesised that Spred proteins play a role in regulating lens development, and that lens epithelial cell proliferation and fibre differentiation are tightly managed by the Spred proteins. To test this, we characterised mice deficient for Spred proteins, systemically and specifically in the eye and/or lens, demonstrating that levels of ERK1/2 phosphorylation are increased, leading to aberrant lens cellular behaviour resulting in impaired lens and eye growth. Characterising the mutant mouse lines deficient for Spred, we demonstrated that Spred 1 and Spred 2 are not necessary for early lens development or for the initial formation of the primary fibre cells, however both proteins are required for the continual growth of the lens and subsequently eye growth. Furthermore, we have established both in vivo and in vitro that Spred proteins are able to antagonise pERK1/2 levels, key enzymes that regulate lens cellular processes, namely epithelial cell proliferation and fibre differentiation. Moreover, we observe an eloquent compensatory mechanism for Spreds with related RTK-antagonists, namely, Sprouty proteins. It is evident from this study that when the regular lens cell cycle and other cellular processes are disrupted, it leads to developmental abnormalities and/or pathology resulting in the loss of lens transparency. From this, we propose that later in life, Spred proteins may indirectly influence TGFβ−induced EMT, through the crosstalk and communication between the ERK/MAPK and TGFβ-Smad signalling pathways. The findings presented in this study implicate an important role for both Spred1 and Spred2 proteins in normal lens and eye development by negatively regulating ERK/MAPK-signalling. Taken together, Spred proteins may be a putative target for developing novel therapeutic-treatments for not only cataract, but other pathologies where cellular proliferation and differentiation is dysregulated.
Subjects/Keywords: spred;
lens development;
cell signalling;
cell proliferation;
cell differentiation;
microphthalmia
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wazin, F. (2020). Spred: a negative regulator of cellular processes involved in lens and eye development
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/22989
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wazin, Fatima. “Spred: a negative regulator of cellular processes involved in lens and eye development
.” 2020. Thesis, University of Sydney. Accessed April 18, 2021.
http://hdl.handle.net/2123/22989.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wazin, Fatima. “Spred: a negative regulator of cellular processes involved in lens and eye development
.” 2020. Web. 18 Apr 2021.
Vancouver:
Wazin F. Spred: a negative regulator of cellular processes involved in lens and eye development
. [Internet] [Thesis]. University of Sydney; 2020. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/2123/22989.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wazin F. Spred: a negative regulator of cellular processes involved in lens and eye development
. [Thesis]. University of Sydney; 2020. Available from: http://hdl.handle.net/2123/22989
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
18.
Kop, Anna.
DNA Methylation Changes at Promoters of Endothelial Cell-enriched Genes during in vitro Differentiation.
Degree: 2011, University of Toronto
URL: http://hdl.handle.net/1807/31285
► This study examined DNA methylation patterns at promoters of endothelial cell (EC)-enriched genes during differentiation of mouse ES cells towards the EC. We have previously…
(more)
▼ This study examined DNA methylation patterns at promoters of endothelial cell (EC)-enriched genes during differentiation of mouse ES cells towards the EC. We have previously shown that eNOS, CD31, VE-cadherin and vWF, which have an EC-enriched pattern of gene expression are differentially methylated between EC and vascular smooth muscle cells. Given that differential promoter DNA methylation is functionally important we asked when these distinct patterns are established. Using the hanging drop method to differentiate ES cells, followed by FACS, we isolated early (EB-day4 VEGFR2-positive) and late (EB-day7 CD31-positive) endothelial progenitor cells. Though current paradigms suggest that lineage-restricted genes are methylated in ES cells, we show heterogeneous promoter DNA methylation. We show DNA demethylation at the CD31 promoter in EB-day 7 CD31-positive cells. In contrast, the eNOS promoter is still heavily methylated in EB-day 7 CD31 positive cells compared with murine EC where there is no DNA methylation.
MAST
Advisors/Committee Members: Marsden, Philip A., Laboratory Medicine and Pathobiology.
Subjects/Keywords: endothelial cell; epigenetics; DNA methylation; differentiation; 0307
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kop, A. (2011). DNA Methylation Changes at Promoters of Endothelial Cell-enriched Genes during in vitro Differentiation. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/31285
Chicago Manual of Style (16th Edition):
Kop, Anna. “DNA Methylation Changes at Promoters of Endothelial Cell-enriched Genes during in vitro Differentiation.” 2011. Masters Thesis, University of Toronto. Accessed April 18, 2021.
http://hdl.handle.net/1807/31285.
MLA Handbook (7th Edition):
Kop, Anna. “DNA Methylation Changes at Promoters of Endothelial Cell-enriched Genes during in vitro Differentiation.” 2011. Web. 18 Apr 2021.
Vancouver:
Kop A. DNA Methylation Changes at Promoters of Endothelial Cell-enriched Genes during in vitro Differentiation. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/1807/31285.
Council of Science Editors:
Kop A. DNA Methylation Changes at Promoters of Endothelial Cell-enriched Genes during in vitro Differentiation. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/31285
19.
Fujita, Muneteru; Nakano, Keisuke; Funato, Akiyoshi; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Okafuji, Norimasa; Hasegawa, Hiromasa.
Heat Shock Protein27 Expression and Cell Differentiation in Ameloblastomas : エナメル上皮腫におけるHeat Shock Protein27の発現と細胞分化.
Degree: 博士（歯学）, 2014, Matsumoto Dental University / 松本歯科大学
URL: http://id.nii.ac.jp/1070/00002235/
► The expression of HSP27 and some CKs were examined the 40 cases of typical solid/multicystic ameloblastoma using immunohistochemical techniques. In order to examine the relevance…
(more)
▼ The expression of HSP27 and some CKs were examined the 40 cases of typical solid/multicystic ameloblastoma using immunohistochemical techniques. In order to examine the relevance of HSP in cell differentiation, we focused on the cytoskeletal expression of CK. CK19 is a marker of typical odontogenic epithelium widely observed in follicular and plexiform types of ameloblastomas. Since staining with CK14 is one of the measures of the differentiation potential of squamous cells and is extensively expressed in both follicular and plexiform types, it implies that squamous differentiation of each type can occur. CK8 was strongly detected in tumor nests in plexiform type but weakly detected in follicular type. It was considered that the expression of HSP27 in plexiform type correlated with the expression of CK8 suggesting that HSP27 might have regulated the expression of CK8.
2013
Subjects/Keywords: ameloblatoma; immunohistochemistry; HSP; CK; cell differentiation; immunohistochemistry
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fujita, Muneteru; Nakano, Keisuke; Funato, Akiyoshi; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Okafuji, Norimasa; Hasegawa, H. (2014). Heat Shock Protein27 Expression and Cell Differentiation in Ameloblastomas : エナメル上皮腫におけるHeat Shock Protein27の発現と細胞分化. (Thesis). Matsumoto Dental University / 松本歯科大学. Retrieved from http://id.nii.ac.jp/1070/00002235/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fujita, Muneteru; Nakano, Keisuke; Funato, Akiyoshi; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Okafuji, Norimasa; Hasegawa, Hiromasa. “Heat Shock Protein27 Expression and Cell Differentiation in Ameloblastomas : エナメル上皮腫におけるHeat Shock Protein27の発現と細胞分化.” 2014. Thesis, Matsumoto Dental University / 松本歯科大学. Accessed April 18, 2021.
http://id.nii.ac.jp/1070/00002235/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fujita, Muneteru; Nakano, Keisuke; Funato, Akiyoshi; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Okafuji, Norimasa; Hasegawa, Hiromasa. “Heat Shock Protein27 Expression and Cell Differentiation in Ameloblastomas : エナメル上皮腫におけるHeat Shock Protein27の発現と細胞分化.” 2014. Web. 18 Apr 2021.
Vancouver:
Fujita, Muneteru; Nakano, Keisuke; Funato, Akiyoshi; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Okafuji, Norimasa; Hasegawa H. Heat Shock Protein27 Expression and Cell Differentiation in Ameloblastomas : エナメル上皮腫におけるHeat Shock Protein27の発現と細胞分化. [Internet] [Thesis]. Matsumoto Dental University / 松本歯科大学; 2014. [cited 2021 Apr 18].
Available from: http://id.nii.ac.jp/1070/00002235/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fujita, Muneteru; Nakano, Keisuke; Funato, Akiyoshi; Sugita, Yoshihiko; Kubo, Katsutoshi; Maeda, Hatsuhiko; Okafuji, Norimasa; Hasegawa H. Heat Shock Protein27 Expression and Cell Differentiation in Ameloblastomas : エナメル上皮腫におけるHeat Shock Protein27の発現と細胞分化. [Thesis]. Matsumoto Dental University / 松本歯科大学; 2014. Available from: http://id.nii.ac.jp/1070/00002235/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Sakai, Kenzo.
Pathological Analysis of Cell Differentiation in Cholesterin Granulomas experimentally induced in Mice : 実験的に誘発したマウスのコレステリン肉芽腫における細胞分化に関する病理学的検討.
Degree: 博士（歯学）, 2016, Matsumoto Dental University / 松本歯科大学
URL: http://id.nii.ac.jp/1070/00002501/
► Cell differentiation in cholesterin granulomas was investigated using ddY mice and GFP bone marrow transplanted mice. Cholesterin was embedded in mice subcutaneously and histopathological examination…
(more)
▼ Cell differentiation in cholesterin granulomas was investigated using ddY mice and GFP bone marrow transplanted mice. Cholesterin was embedded in mice subcutaneously and histopathological examination was carried out in a period of 6 months. Results showed that at 2 weeks, cholesterin was replaced partly by granulation tissues. The majority of cells in the granulation tissues were macrophages and foreign body giant cells and the center consists of small amount of fibroblasts, collagen fibers and capillaries. At 3 months, more granulation tissue was observed compared to 2 weeks. Similar cells were observed, however, there were more fibroblasts, collagen bundles and capillaries present compared to 2 weeks. At 6 months, the cholesterin was mostly substituted by fibrous tissues consisting mainly of fibroblasts and collagen fibers with some macrophages and foreign body giant cells. Specifically, the outer part of the tissue consists of fibroblasts, collagen bundles and capillaries and the inner portion is filled with collagen bundles. Immunohistochemistry revealed that macrophages and foreign body giant cells were positive to GFP and CD68 although the fibroblasts and capillaries in the outer portion of cholesterin granulomas were GFP negative. Some spindle shape fibroblasts were also GFP positive. Immunofluorescent double staining revealed that cells lining the blood vessels were both positive to GFP and CD31 indicating that those were endothelial cells and were actually derived from the transplanted bone marrow cells. The results suggest that macrophages, foreign body giant cells as well as fibroblasts and capillary endothelial cells are bone marrow derived mesenchymal cells.
2015
Subjects/Keywords: Pathological Analysis; Cell Differentiation; Cholesterin Granulomas; Mice
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sakai, K. (2016). Pathological Analysis of Cell Differentiation in Cholesterin Granulomas experimentally induced in Mice : 実験的に誘発したマウスのコレステリン肉芽腫における細胞分化に関する病理学的検討. (Thesis). Matsumoto Dental University / 松本歯科大学. Retrieved from http://id.nii.ac.jp/1070/00002501/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sakai, Kenzo. “Pathological Analysis of Cell Differentiation in Cholesterin Granulomas experimentally induced in Mice : 実験的に誘発したマウスのコレステリン肉芽腫における細胞分化に関する病理学的検討.” 2016. Thesis, Matsumoto Dental University / 松本歯科大学. Accessed April 18, 2021.
http://id.nii.ac.jp/1070/00002501/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sakai, Kenzo. “Pathological Analysis of Cell Differentiation in Cholesterin Granulomas experimentally induced in Mice : 実験的に誘発したマウスのコレステリン肉芽腫における細胞分化に関する病理学的検討.” 2016. Web. 18 Apr 2021.
Vancouver:
Sakai K. Pathological Analysis of Cell Differentiation in Cholesterin Granulomas experimentally induced in Mice : 実験的に誘発したマウスのコレステリン肉芽腫における細胞分化に関する病理学的検討. [Internet] [Thesis]. Matsumoto Dental University / 松本歯科大学; 2016. [cited 2021 Apr 18].
Available from: http://id.nii.ac.jp/1070/00002501/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sakai K. Pathological Analysis of Cell Differentiation in Cholesterin Granulomas experimentally induced in Mice : 実験的に誘発したマウスのコレステリン肉芽腫における細胞分化に関する病理学的検討. [Thesis]. Matsumoto Dental University / 松本歯科大学; 2016. Available from: http://id.nii.ac.jp/1070/00002501/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
21.
Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Mada, Hatsuhiko; Hasegawa, Hiromasa; Kawakami, Toshiyuki.
Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas : 多形腺腫における細胞分化因子としてのNotchの可能性.
Degree: 博士（歯学）, 2016, Matsumoto Dental University / 松本歯科大学
URL: http://id.nii.ac.jp/1070/00002504/
► The expression of Notch in 30 cases of pleomorphic adenoma was examined by immunohistochemistry.Comparing the results of our study with previous literatures, from the partial…
(more)
▼ The expression of Notch in 30 cases of pleomorphic adenoma was examined by immunohistochemistry.Comparing the results of our study with previous literatures, from the partial CK7 expression and substantial Notch expression in ductal epithelial cells as well as the Notch expression in solid tumor nests, it can be inferred that Notch is involved in cell differentiation. CK13 expression was observed in cells undergoing squamous metaplasia and Notch expression was seen in the nucleus of basal and squamous cells. The intense Notch expression in basal cells and weak expression in squamous cells suggests that Notch is involved in the differentiation from basal to squamous cell. Moreover, the loss of nuclear expression on the inner layer would signify that differentiation is about to end or has been terminated. Notch was expressed in the cytoplasm of cartilage cells and in the cell membrane of mucous cells but not in the nucleus indicating that differentiation has been concluded. Notch involvement is suspected in cell differentiation in areas showing ductal structures and squamous metaplasia. In summary, Notch is involved in cell differentiation of ductal cells in PA. Nuclear expression was shown in tumor cells in solid nests and surrounding structures. Moreover, Notch is expressed by basal cells undergoing squamous metaplasia suggesting the participation of Notch in cell differentiation in PA.
2015
Subjects/Keywords: Notch1; pleomorphic adenoma; cell differentiation; immunohistochemistry
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Mada, Hatsuhiko; Hasegawa, Hiromasa; Kawakami, T. (2016). Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas : 多形腺腫における細胞分化因子としてのNotchの可能性. (Thesis). Matsumoto Dental University / 松本歯科大学. Retrieved from http://id.nii.ac.jp/1070/00002504/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Mada, Hatsuhiko; Hasegawa, Hiromasa; Kawakami, Toshiyuki. “Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas : 多形腺腫における細胞分化因子としてのNotchの可能性.” 2016. Thesis, Matsumoto Dental University / 松本歯科大学. Accessed April 18, 2021.
http://id.nii.ac.jp/1070/00002504/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Mada, Hatsuhiko; Hasegawa, Hiromasa; Kawakami, Toshiyuki. “Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas : 多形腺腫における細胞分化因子としてのNotchの可能性.” 2016. Web. 18 Apr 2021.
Vancouver:
Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Mada, Hatsuhiko; Hasegawa, Hiromasa; Kawakami T. Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas : 多形腺腫における細胞分化因子としてのNotchの可能性. [Internet] [Thesis]. Matsumoto Dental University / 松本歯科大学; 2016. [cited 2021 Apr 18].
Available from: http://id.nii.ac.jp/1070/00002504/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Takamine, Keisuke; Ueda, Yukiko; Nakano, Keisuke; Ochiai, Takanaga; Sugita, Yoshihiko; Kubo, Katsutoshi; Mada, Hatsuhiko; Hasegawa, Hiromasa; Kawakami T. Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas : 多形腺腫における細胞分化因子としてのNotchの可能性. [Thesis]. Matsumoto Dental University / 松本歯科大学; 2016. Available from: http://id.nii.ac.jp/1070/00002504/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
22.
MATSUDA, SAEKA; SHOMURA, MASAHITO; OSUGA, NAOTO; TSUJIGIWA, HIDETSUGU; NAKANO, KEISUKE; OKAFUJI, NORIMASA; OCHIAI, TAKANAGA; HASEGAWA, HIROMASA; KAWAKAMI, TOSHIYUKI.
Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice : マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化.
Degree: 博士（歯学）, 2017, Matsumoto Dental University / 松本歯科大学
URL: http://id.nii.ac.jp/1070/00002655/
► Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large,…
(more)
▼ Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large, granulation tissue would grow to form periodontal polyp. Granulation tissue consists of proliferating cells however their origin is not clear. It was shown that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow. Hence, this study utilized GFP bone marrow transplantation mouse model. The floor of the pulp chamber in maxillary first molar was perforated using ½ dental round bur. Morphological assessment was carried out by micro CT and microscopy and GFP cell mechanism was further assessed by immunohistochemistry using double fluorescent staining with GFP-S100A4; GFP-Runx2 and GFP-CD31. Results of micro CT revealed alveolar bone resorption and widening of periodontal ligament. Histopathological examination showed proliferation of fibroblasts with some round cells and blood vessels in the granulation tissue. At 2 weeks, the outermost layer of the granulation tissue was lined by squamous cells with distinct intercellular bridges. At 4 weeks, the granulation tissue became larger than the perforation and the outermost layer was lined by relatively typical stratified squamous epithelium. Double immunofluorescent staining of GFP and Runx2 revealed that both proteins were expressed in spindle-shaped cells. Double immunofluorescent staining of GFP and CD31 revealed that both proteins were expressed in vascular endothelial cells in morphologically distinct vessels. The results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury.
2016
Subjects/Keywords: Periodontal polyp; Periodontal ligament; Cell differentiation; Immunohistochemistry
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
MATSUDA, SAEKA; SHOMURA, MASAHITO; OSUGA, NAOTO; TSUJIGIWA, HIDETSUGU; NAKANO, KEISUKE; OKAFUJI, NORIMASA; OCHIAI, TAKANAGA; HASEGAWA, HIROMASA; KAWAKAMI, T. (2017). Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice : マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化. (Thesis). Matsumoto Dental University / 松本歯科大学. Retrieved from http://id.nii.ac.jp/1070/00002655/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
MATSUDA, SAEKA; SHOMURA, MASAHITO; OSUGA, NAOTO; TSUJIGIWA, HIDETSUGU; NAKANO, KEISUKE; OKAFUJI, NORIMASA; OCHIAI, TAKANAGA; HASEGAWA, HIROMASA; KAWAKAMI, TOSHIYUKI. “Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice : マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化.” 2017. Thesis, Matsumoto Dental University / 松本歯科大学. Accessed April 18, 2021.
http://id.nii.ac.jp/1070/00002655/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
MATSUDA, SAEKA; SHOMURA, MASAHITO; OSUGA, NAOTO; TSUJIGIWA, HIDETSUGU; NAKANO, KEISUKE; OKAFUJI, NORIMASA; OCHIAI, TAKANAGA; HASEGAWA, HIROMASA; KAWAKAMI, TOSHIYUKI. “Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice : マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化.” 2017. Web. 18 Apr 2021.
Vancouver:
MATSUDA, SAEKA; SHOMURA, MASAHITO; OSUGA, NAOTO; TSUJIGIWA, HIDETSUGU; NAKANO, KEISUKE; OKAFUJI, NORIMASA; OCHIAI, TAKANAGA; HASEGAWA, HIROMASA; KAWAKAMI T. Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice : マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化. [Internet] [Thesis]. Matsumoto Dental University / 松本歯科大学; 2017. [cited 2021 Apr 18].
Available from: http://id.nii.ac.jp/1070/00002655/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
MATSUDA, SAEKA; SHOMURA, MASAHITO; OSUGA, NAOTO; TSUJIGIWA, HIDETSUGU; NAKANO, KEISUKE; OKAFUJI, NORIMASA; OCHIAI, TAKANAGA; HASEGAWA, HIROMASA; KAWAKAMI T. Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice : マウスにおける実験的に発生させた歯根膜ポリープへのGFP移植骨髄由来細胞の移動と分化. [Thesis]. Matsumoto Dental University / 松本歯科大学; 2017. Available from: http://id.nii.ac.jp/1070/00002655/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
23.
Suresh, Hamsini.
Homeostatic mechanics framework for modelling interactions of cells with the microenvironment.
Degree: PhD, 2019, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/297846
► Spreading and locomotion enable the cells to explore their microenvironments during the interphase period of the cell cycle (24-48 hours after cell seeding and prior…
(more)
▼ Spreading and locomotion enable the cells to explore their microenvironments during the interphase period of the cell cycle (24-48 hours after cell seeding and prior to cell division). The morphologies adopted by cells in this period dictate orientational ordering, direction and speed of migration, lineage of differentiation and apoptosis. It is well established that cells display a fluctuating response in their microenvironments, and no two cells evolve in the same manner despite being cultured under identical conditions. However, the statistics of cell spreading (i.e. morphometrics such as cell area, aspect ratio, etc.) are remarkably reproducible when experiments are performed over a large number of cells. A mechanistic understanding of this stochastic behaviour of cells will have far-reaching implications in aiding the interpretation of a wide range of cell functionalities.
The aim of this thesis is to model the response of single cells to mechanical, chemical and geometric cues in their microenvironment. In the first part of the thesis, we study the contact guidance of myofibroblasts on adhesive stripes of different widths. Contact guidance—the widely-known phenomenon of cell alignment induced by anisotropic environmental features—plays a crucial role in the micro-architecture of tissues and dictates their biological and mechanical functioning. However, the mechanisms by which cells achieve this orientational ordering remain unclear. To examine the mechanisms underpinning contact guidance, we combine detailed morphometric analysis of cells and their subcellular components with the novel homeostatic mechanics framework that recognises the non-thermal fluctuating response of living cells. We show that similar to nematic ordering in liquid crystals, orientational ordering of cells on micropatterns is driven, rather counter-intuitively, by the tendency of cells to maximise morphological disorder. This finding reveals an alternative, entropy-mediated mechanism for explaining the response of cells to anisotropic environmental cues besides the often-invoked mechanisms that are predicated on very specific biochemical feedback pathways.
In the second part of the thesis, we extend the framework to investigate the role of substrate curvature in the orientational ordering of myofibroblasts. We propose that the microtubules are the primary cytoskeletal component involved in curvature sensing. Using detailed morphometric analysis, we confirm our hypothesis, and additionally verify that the microtubules control the patterns of stress-fibres formed in cells, in turn controlling cell polarisation and alignment. We also observe that the absence of microtubules results in reduced guidance on the curved substrates, concomitant with increased cellular traction forces.
The variability in morphometrics (such as cell shape, area, cytoskeletal protein arrangements and traction forces) also reflects in other critical cell functionality. In particular, the fluctuating response of stem cells on mechanical, chemical and geometric…
Subjects/Keywords: Statistical mechanics; contact guidance; stem cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Suresh, H. (2019). Homeostatic mechanics framework for modelling interactions of cells with the microenvironment. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/297846
Chicago Manual of Style (16th Edition):
Suresh, Hamsini. “Homeostatic mechanics framework for modelling interactions of cells with the microenvironment.” 2019. Doctoral Dissertation, University of Cambridge. Accessed April 18, 2021.
https://www.repository.cam.ac.uk/handle/1810/297846.
MLA Handbook (7th Edition):
Suresh, Hamsini. “Homeostatic mechanics framework for modelling interactions of cells with the microenvironment.” 2019. Web. 18 Apr 2021.
Vancouver:
Suresh H. Homeostatic mechanics framework for modelling interactions of cells with the microenvironment. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Apr 18].
Available from: https://www.repository.cam.ac.uk/handle/1810/297846.
Council of Science Editors:
Suresh H. Homeostatic mechanics framework for modelling interactions of cells with the microenvironment. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/297846
University of Cambridge
24.
Capatina, Nadejda.
Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation.
Degree: PhD, 2019, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/298767
► The project aimed to provide a mechanistic understanding of the effect of endoplasmic reticulum (ER) stress on the differentiation of trophoblast stem cells (TSCs), placental…
(more)
▼ The project aimed to provide a mechanistic understanding of the effect of endoplasmic reticulum (ER) stress on the differentiation of trophoblast stem cells (TSCs), placental development, and subsequent pregnancy outcome.
To address this question, I first used an in vitro mouse TSC model. Morphological observations suggest that upon ER stress, TSCs lose stemness by differentiating into specialized trophoblast cell subtypes. Second, I used an in vivo Eif2s1tm1RjK mouse model that exhibits chronic ER stress.Third, I induced ER stress using a more physiological stress inducer in pre-implantation wild-type embryos for 48h in vitro, which results in a significantly smaller TE stem cell pool, and increased expression of Grp78, Atf4 and Atf6a UPR markers. The phenotype was partially rescued by addition of a chaperone to the culture medium.
In conclusion, both the in vitro and in vivo models demonstrate that ER stress perturbs differentiation of TS cells at the blastocyst stage, with consequences for placental morphogenesis and fetal development. Pilot studies using a stress-relieving chaperone indicate that therapeutic interventions may be possible.
Subjects/Keywords: endoplasmic reticulum stress; trophoblast; stem cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Capatina, N. (2019). Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/298767
Chicago Manual of Style (16th Edition):
Capatina, Nadejda. “Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation.” 2019. Doctoral Dissertation, University of Cambridge. Accessed April 18, 2021.
https://www.repository.cam.ac.uk/handle/1810/298767.
MLA Handbook (7th Edition):
Capatina, Nadejda. “Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation.” 2019. Web. 18 Apr 2021.
Vancouver:
Capatina N. Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Apr 18].
Available from: https://www.repository.cam.ac.uk/handle/1810/298767.
Council of Science Editors:
Capatina N. Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/298767
Columbia University
25.
Yudanin, Naomi Ava.
Memory T cell compartmentalization, maintenance, and retention.
Degree: 2015, Columbia University
URL: https://doi.org/10.7916/D8C24VM2
► Pathways and mechanisms for human memory T cell differentiation and maintenance have largely been inferred from studies of peripheral blood, though the majority of T…
(more)
▼ Pathways and mechanisms for human memory T cell differentiation and maintenance have largely been inferred from studies of peripheral blood, though the majority of T cells are found in lymphoid and mucosal sites. We present here a novel, multidimensional, quantitative analysis of human T cell compartmentalization and maintenance over six decades of life in blood, lymphoid and mucosal tissues obtained from 56 individual organ donors. Our results reveal that the distribution and tissue residence of naïve, central and effector memory, and terminal effector subsets is contingent on both differentiation state and tissue localization. Moreover, T cell homeostasis driven by cytokine or TCR-mediated signals is dependent on CD4+ or CD8+ T cell lineage, subset differentiation and tissue localization, and cannot be inferred from blood. Our data provide an unprecedented spatial and temporal map of human T cell compartmentalization and maintenance, supporting new pathways for human T cell fate determination and homeostasis.
Memory T cells can remain in tissues as non-circulating, resident memory populations, which provide optimal protection against infection at barrier surfaces. Lung-resident memory T cells (TRM) mediate in situ protection to respiratory pathogens, though mechanisms for their maintenance and retention are unknown. Through whole transcriptome profiling, we identify a cohesive network of genes enriched in lung CD4+ TRM, including Itgad (CD11d), Cd69, and IFN-associated responders. We find that upregulation of CD11d enhances CD69 expression through type I IFN signaling downstream of homotypic cell adhesion, and is required for optimal T cell differentiation and lung retention. Moreover, blockade of IFNαR1 reduces CD11d expression and retention of influenza-generated lung TRM, suggesting that CD11d-dependent type I IFN signaling promotes TRM establishment. Our results implicate CD11d and type I IFN in retaining lung CD4+ TRM cells, and identify potential targets for modulating tissue immunity.
Subjects/Keywords: Cell compartmentation; Immunology; T cells – Differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yudanin, N. A. (2015). Memory T cell compartmentalization, maintenance, and retention. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8C24VM2
Chicago Manual of Style (16th Edition):
Yudanin, Naomi Ava. “Memory T cell compartmentalization, maintenance, and retention.” 2015. Doctoral Dissertation, Columbia University. Accessed April 18, 2021.
https://doi.org/10.7916/D8C24VM2.
MLA Handbook (7th Edition):
Yudanin, Naomi Ava. “Memory T cell compartmentalization, maintenance, and retention.” 2015. Web. 18 Apr 2021.
Vancouver:
Yudanin NA. Memory T cell compartmentalization, maintenance, and retention. [Internet] [Doctoral dissertation]. Columbia University; 2015. [cited 2021 Apr 18].
Available from: https://doi.org/10.7916/D8C24VM2.
Council of Science Editors:
Yudanin NA. Memory T cell compartmentalization, maintenance, and retention. [Doctoral Dissertation]. Columbia University; 2015. Available from: https://doi.org/10.7916/D8C24VM2
Columbia University
26.
Stupnikov, Maria Rose.
Genetic regulation of pulmonary progenitor cell differentiation.
Degree: 2019, Columbia University
URL: https://doi.org/10.7916/d8-kgxh-ga80
► The respiratory system represents a major interface between the body and the external environment. Its design includes a tree-like network of conducting tubules (airways) that…
(more)
▼ The respiratory system represents a major interface between the body and the external environment. Its design includes a tree-like network of conducting tubules (airways) that carries air to millions of alveoli, where gas exchange occurs. The conducting airways are characterized by their great diversity in epithelial cell types with multiple populations of secretory, multiciliated, and neuroendocrine cells. How these different cell types arise and how these populations are balanced are questions still not well understood. Aberrant patterns of airway epithelial differentiation have been described in various human pulmonary diseases, chronic bronchitis, asthma, neuroendocrine hyperplasia of infancy, and others.
The goal of this thesis is to investigate mechanisms of regulation of airway epithelial cell fate in the developing lung epithelium. More specifically, these studies focus on Notch signaling and address a long unresolved issue whether the different Notch ligands (Jagged and Delta) have distinct roles in the epithelial differentiation program of the extrapulmonary and intrapulmonary airways. Moreover, these studies investigate the ontogeny of the bHLH transcription factor Ascl1 and identify its targets in the developing airways as potential regulators of neuroepithelial body (NEB) size and maturation.
My studies provide evidence that the Notch ligand families Jag and Dll are required for the specification and formation of different cell lineages in the developing airway epithelia. Jag ligands regulate multiciliated versus secretory (club) cell fates but also controls abundance of basal cell progenitors in extrapulmonary airways. Dll ligands regulate pulmonary neuroendocrine versus club cell fates in intrapulmonary airways. Analysis of mouse mutants showed that loss of Jag ligands has minimal impact on the size or abundance of NEBs and their associated secretory cells while loss of Dll ligands results in an expansion of NEB size and associated cells. To gain additional insights into the potential mechanisms of how neuroendocrine cells develop and undergo aberrant hyperplasia, I characterized the global transcriptional profile of embryonic lungs from mice deficient in Ascl1, which lack NEBs and neuroendocrine cells and identified a number of genes associated with neuroendocrine cell development, maturation, and the NEB microenvironment. Among these genes, components of the catecholamine biosynthesis pathway, such as tyrosine hydroxylase (Th), a key enzyme for catecholamine production, were downregulated in Ascl1 null lungs. Subsequent functional analysis using a pharmacological inhibitor of this pathway in lung organ cultures showed expansion of pulmonary neuroendocrine cells and NEB size, an observation of potential relevance in human diseases in which neuroendocrine cells are aberrantly expanded.
Together these studies highlight the distinct role of Notch ligands and further implicate Ascl1 targets, as illustrated by catecholamine pathway components, in regulating epithelial cell fate. Further…
Subjects/Keywords: Genetic regulation; Stem cells; Cell differentiation; Lungs
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Stupnikov, M. R. (2019). Genetic regulation of pulmonary progenitor cell differentiation. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/d8-kgxh-ga80
Chicago Manual of Style (16th Edition):
Stupnikov, Maria Rose. “Genetic regulation of pulmonary progenitor cell differentiation.” 2019. Doctoral Dissertation, Columbia University. Accessed April 18, 2021.
https://doi.org/10.7916/d8-kgxh-ga80.
MLA Handbook (7th Edition):
Stupnikov, Maria Rose. “Genetic regulation of pulmonary progenitor cell differentiation.” 2019. Web. 18 Apr 2021.
Vancouver:
Stupnikov MR. Genetic regulation of pulmonary progenitor cell differentiation. [Internet] [Doctoral dissertation]. Columbia University; 2019. [cited 2021 Apr 18].
Available from: https://doi.org/10.7916/d8-kgxh-ga80.
Council of Science Editors:
Stupnikov MR. Genetic regulation of pulmonary progenitor cell differentiation. [Doctoral Dissertation]. Columbia University; 2019. Available from: https://doi.org/10.7916/d8-kgxh-ga80
Queen Mary, University of London
27.
Shephard, Matthew T.
Targeted differentiation of pluripotent stem cells to hepatocytes.
Degree: PhD, 2018, Queen Mary, University of London
URL: http://qmro.qmul.ac.uk/xmlui/handle/123456789/56077
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.786338
► Pluripotent stem cells (PSCs) possess the ability to differentiate to virtually any cell type whilst retaining the capacity to self-renew. There is an unmet need…
(more)
▼ Pluripotent stem cells (PSCs) possess the ability to differentiate to virtually any cell type whilst retaining the capacity to self-renew. There is an unmet need for an inexhaustible supply of hepatocytes to perform drug toxicity and metabolism screens on early stage drugs. PSCs therefore hold potential to generate mature hepatocytes for pharmaceutical testing. To realise this potential, there is a requirement to recapitulate hepatocyte specification in vitro. There are two main bottlenecks in generating metabolically relevant hepatocytes. The first is the specification of definitive endoderm (DE), where it is known that the TGFβ family member Nodal is the key driver in vivo. Currently the closely related TGFβ family member Activin A (AA) is universally used to mimic this process. However, it has been observed that AA results in off-target gene modulation that may be deleterious to the production of DE; from which the hepatic lineage arises. Preliminary data from the McKay group has shown that AA stimulates endogenous Nodal activity when applied to PSCs; leading to the hypothesis that Nodal is the true driver of DE specification in vitro. To address this avenue of investigation, tools to modulate Nodal signalling were assessed in different cell culture systems. Findings included the need to use an appropriate viral promotor to efficiently express lentiviral vectors in PSCs. The second major bottleneck concerns hepatic maturation where the signalling pathways and key regulators in vitro are not fully understood. Consequently, current derived hepatocyte-like cells (HLCs) show low functionality. Utilising Plasticell's CombiCult® allowed thousands of growth factor and small molecule combinations to be screened to define efficient differentiation protocols. Protocols were derived containing novel differentiation factors that give rise to CYP450 inducible HLCs. Lentiviral reporters were then used to determine optimum concentrations of small molecules and to test ligands for the upregulation of hepatic master regulators. Findings gained from this thesis have contributed new insights into the differentiation of PSCs to hepatocytes.
Subjects/Keywords: Pluripotent Stem Cells; hepatocytes; cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shephard, M. T. (2018). Targeted differentiation of pluripotent stem cells to hepatocytes. (Doctoral Dissertation). Queen Mary, University of London. Retrieved from http://qmro.qmul.ac.uk/xmlui/handle/123456789/56077 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.786338
Chicago Manual of Style (16th Edition):
Shephard, Matthew T. “Targeted differentiation of pluripotent stem cells to hepatocytes.” 2018. Doctoral Dissertation, Queen Mary, University of London. Accessed April 18, 2021.
http://qmro.qmul.ac.uk/xmlui/handle/123456789/56077 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.786338.
MLA Handbook (7th Edition):
Shephard, Matthew T. “Targeted differentiation of pluripotent stem cells to hepatocytes.” 2018. Web. 18 Apr 2021.
Vancouver:
Shephard MT. Targeted differentiation of pluripotent stem cells to hepatocytes. [Internet] [Doctoral dissertation]. Queen Mary, University of London; 2018. [cited 2021 Apr 18].
Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/56077 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.786338.
Council of Science Editors:
Shephard MT. Targeted differentiation of pluripotent stem cells to hepatocytes. [Doctoral Dissertation]. Queen Mary, University of London; 2018. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/56077 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.786338
Queen Mary, University of London
28.
Zhao, Hanqing.
MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG binding protein 2.
Degree: PhD, 2015, Queen Mary, University of London
URL: http://qmro.qmul.ac.uk/xmlui/handle/123456789/12967
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775178
► In recent years, microRNAs have emerged as important regulators in various biological processes, as a new class of biomarkers, and as novel therapeutic drugs for…
(more)
▼ In recent years, microRNAs have emerged as important regulators in various biological processes, as a new class of biomarkers, and as novel therapeutic drugs for many diseases, including cardiovascular diseases. Tumour suppressor microRNA-22 (miRNA-22 or miR-22) has been reported to regulate cardiac aging and to play a role in hematopoietic cell differentiation and maturation. Moreover, DNA methylation, a major modification of eukaryotic genomes, plays an essential role in mammalian development. Methyl CpG-binding protein 2 (MECP2) is capable of binding specifically to methylated DNA and is involved in gene silencing. The main objectives of this PhD project were to determine the functional impact of miRNA-22 and its target gene, MECP2, in smooth muscle cell (SMC) differentiation and to delineate the molecular mechanism involved. Mouse embryonic stem (ES) cells were seeded on collagen-coated flasks in differentiation medium to promote SMC differentiation. MiRNA-22 was significantly upregulated during SMC differentiation from ES cells. Enforced expression of miRNA-22 by its mimic or knockdown of miR-22 by its antagomiR promoted or inhibited SMC differentiation from ES cells, respectively. As expected, miRNA-22 overexpression in stem cells promoted SMC differentiation in vivo. Consistently, a similar change in miR-22 expression and a similar functional role for miRNA-22 were observed during SMC differentiation from adventitia stem/progenitor cells isolated from murine blood vessels. MECP2, the founding member of the family of methyl CpG binding domain proteins, was identified by several computational miRNA target prediction tools as one of the top targets of miR-22. Methyl CpG binding domain proteins bind specifically to methylated and unmethylated DNA and recruit distinct interacting protein partners to establish a repressive or active chromatin - 9 - environment. Interestingly, expression of the gene encoding MECP2 decreased significantly during SMC differentiation. MECP2 decreased dramatically in miRNA-22-overexpressing cells, but significantly increased after miRNA-22 knockdown in differentiating stem cells. Moreover, luciferase assays showed that miR-22 substantially inhibited wild-type, but not mutant, MECP2-3'-UTR luciferase activity. In addition, modulation of MECP2 expression levels affected the expression of multiple SMC-specific marker genes in differentiated ES cells. At the mechanistic level, our data showed that MECP2 transcriptionally repressed SMC gene expression by modulating various SMC transcription factors as well as several established SMC differentiation regulators. Additionally, enrichment of H3K9 trimethylation around the promoter regions of SMC transcription factors and other known differentiation regulator genes increased after MECP2 overexpression, suggesting that modulation of DNA methylation is another mechanism underlying MECP2-mediated gene expression during SMC differentiation from stem cells. Finally, miR-22 was upregulated by platelet-derived growth factor-BB and transforming growth…
Subjects/Keywords: Medicine; microRNAs; smooth muscle cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhao, H. (2015). MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG binding protein 2. (Doctoral Dissertation). Queen Mary, University of London. Retrieved from http://qmro.qmul.ac.uk/xmlui/handle/123456789/12967 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775178
Chicago Manual of Style (16th Edition):
Zhao, Hanqing. “MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG binding protein 2.” 2015. Doctoral Dissertation, Queen Mary, University of London. Accessed April 18, 2021.
http://qmro.qmul.ac.uk/xmlui/handle/123456789/12967 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775178.
MLA Handbook (7th Edition):
Zhao, Hanqing. “MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG binding protein 2.” 2015. Web. 18 Apr 2021.
Vancouver:
Zhao H. MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG binding protein 2. [Internet] [Doctoral dissertation]. Queen Mary, University of London; 2015. [cited 2021 Apr 18].
Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/12967 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775178.
Council of Science Editors:
Zhao H. MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG binding protein 2. [Doctoral Dissertation]. Queen Mary, University of London; 2015. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/12967 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775178
University of New Mexico
29.
Wilson, Melissa R.
ANALYSIS OF GENES REQUIRED FOR QUIESCENT CELL FORMATION IN STATIONARY PHASE CULTURES OF SACCHAROMYCES CEREVISIAE.
Degree: UNM Biology Department, 2014, University of New Mexico
URL: https://digitalrepository.unm.edu/biol_etds/114
► Yeast cells in stationary phase cultures, after several days growth in rich, glucose-based medium (YPD), are separable by density-gradient centrifugation into two fractions. The heavier,…
(more)
▼ Yeast cells in stationary phase cultures, after several days growth in rich, glucose-based medium (YPD), are separable by density-gradient centrifugation into two fractions. The heavier, quiescent cells are mostly virgin daughters whereas the less-dense, non-quiescent cells, are a typical mixture of daughters to aged cells. Quiescent cells can also be separated based on expression of specific GFP-tagged proteins, including many that are localized to the mitochodria. To ask the question, what genes are required for this
differentiation process, we used a combination of the diploid, homozygous yeast deletion set, the heterozygous deletion set (carrying one deleted 'essential' gene) and a third set designed to reduce mRNA abundance of a number of 'essential' genes. Samples from the cultures just prior to the diauxic shift (just prior to glucose exhaustion), stationary phase, and isolated quiescent (Q) and nonquiescent (NQ) cells were harvested and technical and biological replicates analyzed by microarray analysis. The results showed that deletions in more than 500 genes resulted in 2-fold or greater reduction in Q-
cell formation. Thus, almost 10% of genes in the yeast genome were important for Q-
cell formation. When mutants with a 2-fold in Q vs all other samples were compared, 411 genes were identified that were important for Q cells vs DS, NQ, and SP. These genes encoded proteins involved in mitochondrial function, protein localization, and vesicle transport. We concluded from these results that
differentiation of quiescent cells requires a major cellular commitment and that the major functions required are similar to those identified by proteomic and transcriptomic analysis of Q cells done previously in our laboratory, furthering understanding of
cell differentiation.
Advisors/Committee Members: Werner-Washburne, Maggie, Natvig, Don, Northup, Diana.
Subjects/Keywords: yeast; Saccharomyces cerevisiae; quiescence; cell differentiation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wilson, M. R. (2014). ANALYSIS OF GENES REQUIRED FOR QUIESCENT CELL FORMATION IN STATIONARY PHASE CULTURES OF SACCHAROMYCES CEREVISIAE. (Masters Thesis). University of New Mexico. Retrieved from https://digitalrepository.unm.edu/biol_etds/114
Chicago Manual of Style (16th Edition):
Wilson, Melissa R. “ANALYSIS OF GENES REQUIRED FOR QUIESCENT CELL FORMATION IN STATIONARY PHASE CULTURES OF SACCHAROMYCES CEREVISIAE.” 2014. Masters Thesis, University of New Mexico. Accessed April 18, 2021.
https://digitalrepository.unm.edu/biol_etds/114.
MLA Handbook (7th Edition):
Wilson, Melissa R. “ANALYSIS OF GENES REQUIRED FOR QUIESCENT CELL FORMATION IN STATIONARY PHASE CULTURES OF SACCHAROMYCES CEREVISIAE.” 2014. Web. 18 Apr 2021.
Vancouver:
Wilson MR. ANALYSIS OF GENES REQUIRED FOR QUIESCENT CELL FORMATION IN STATIONARY PHASE CULTURES OF SACCHAROMYCES CEREVISIAE. [Internet] [Masters thesis]. University of New Mexico; 2014. [cited 2021 Apr 18].
Available from: https://digitalrepository.unm.edu/biol_etds/114.
Council of Science Editors:
Wilson MR. ANALYSIS OF GENES REQUIRED FOR QUIESCENT CELL FORMATION IN STATIONARY PHASE CULTURES OF SACCHAROMYCES CEREVISIAE. [Masters Thesis]. University of New Mexico; 2014. Available from: https://digitalrepository.unm.edu/biol_etds/114
University of Hong Kong
30.
Chan, Sze-wing, Scarlet.
Erythroleukemic cell
differentiation factor (EDF): biochemical, cloning, molecular
structure, and functionalstudies.
Degree: 2000, University of Hong Kong
URL: http://hdl.handle.net/10722/31906
Subjects/Keywords: Cell
differentiation.;
Erythrocytes.
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chan, Sze-wing, S. (2000). Erythroleukemic cell
differentiation factor (EDF): biochemical, cloning, molecular
structure, and functionalstudies. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/31906
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chan, Sze-wing, Scarlet. “Erythroleukemic cell
differentiation factor (EDF): biochemical, cloning, molecular
structure, and functionalstudies.” 2000. Thesis, University of Hong Kong. Accessed April 18, 2021.
http://hdl.handle.net/10722/31906.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chan, Sze-wing, Scarlet. “Erythroleukemic cell
differentiation factor (EDF): biochemical, cloning, molecular
structure, and functionalstudies.” 2000. Web. 18 Apr 2021.
Vancouver:
Chan, Sze-wing S. Erythroleukemic cell
differentiation factor (EDF): biochemical, cloning, molecular
structure, and functionalstudies. [Internet] [Thesis]. University of Hong Kong; 2000. [cited 2021 Apr 18].
Available from: http://hdl.handle.net/10722/31906.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chan, Sze-wing S. Erythroleukemic cell
differentiation factor (EDF): biochemical, cloning, molecular
structure, and functionalstudies. [Thesis]. University of Hong Kong; 2000. Available from: http://hdl.handle.net/10722/31906
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
◁ [1] [2] [3] [4] [5] … [36] ▶
. 
Open Access Theses and Dissertations