You searched for subject:(Cell cell interaction).
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Ryerson University
1.
Pashang, Roshanak.
Bacterial survival at solid-air interfaces: from individual response to population ecology.
Degree: 2016, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A5852 
► Acclimation and flexible response mechanisms are ancient survival modes allowing prokaryotic cells to conquer diverse habitats and maintain viability in nature. Evidently, lack of water…
(more)
▼ Acclimation and flexible response mechanisms are ancient survival modes allowing prokaryotic cells to conquer diverse habitats and maintain viability in nature. Evidently, lack of water significantly impacts cellular response making it vital for individual cells to benefit from population support and access survival means available beyond the cell boundaries. Microscopy of dried bacterial cells at solid-air interfaces suggested that cell membrane breakdown didn’t occur for months. Viable counts for Gram-positive Arthrobacter spp. and Gram-negative Pseudomonas stutzeri were significantly higher for larger population density during prolonged periods of desiccation and nutrient starvation. Presence of homogenized cells extended short-term survival of Arthrobacter spp. cells, while heat-killed cells significantly extended viability of all populations. It appeared that huddled zombielike cells allow preservation and recycling of essential survival components within a population. Understanding population-mediated survival at solid-air interfaces is key for monitoring and controlling bacteria including in deep geological repositories for nuclear waste management.
Subjects/Keywords: Bacteria; Cell interaction
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APA (6th Edition):
Pashang, R. (2016). Bacterial survival at solid-air interfaces: from individual response to population ecology. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A5852
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pashang, Roshanak. “Bacterial survival at solid-air interfaces: from individual response to population ecology.” 2016. Thesis, Ryerson University. Accessed January 21, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A5852.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pashang, Roshanak. “Bacterial survival at solid-air interfaces: from individual response to population ecology.” 2016. Web. 21 Jan 2021.
Vancouver:
Pashang R. Bacterial survival at solid-air interfaces: from individual response to population ecology. [Internet] [Thesis]. Ryerson University; 2016. [cited 2021 Jan 21].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A5852.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pashang R. Bacterial survival at solid-air interfaces: from individual response to population ecology. [Thesis]. Ryerson University; 2016. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A5852
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Florida Atlantic University
2.
Qureshi, Anila.
The novel function of sJAM-C in promoting cytoskeleton rearrangement and migration in mammary epithelial cells.
Degree: MS, 2012, Florida Atlantic University
URL: http://purl.flvc.org/fcla/dt/3362046
► Summary: Soluble form of Junctional adhesion molecule C (sJAM-C) has been identified to cause angiogenesis as well as chemotaxis in endothelial cells. However, the role…
(more)
▼ Summary: Soluble form of Junctional adhesion molecule C (sJAM-C) has been identified to cause angiogenesis as well as chemotaxis in endothelial cells. However, the role of sJAM-C in the context of cancer has not been elucidated. Our atomic force microscopy (AFM) stiffness measurements of normal mammary epithelial cells (MCF 10A) have shown a two-fold decrease in cell's stiffness in response to sJAM-C. Changes in cell stiffness are indicative of modulations in a cell's mechanical properties. Our results indicated that sJAM-C increased the MCF 10A cell migration about two-fold and also promoted a three-fold increase in chemotaxis. Additionally, sJAM-C treatment resulted in considerable filamentous-actin loss and peripheral actin ring breakage. We also found activation of Rho signaling pathway to be the main mechanism behind sJAM-C mediated alterations in MCF 10A cell cytoskeleton and motility. Our data present for the first time that sJAM-C is a pro metastatic mediator for normal mammary epithelial cells.
System requirements: Adobe Reader.
Subjects/Keywords: Tight junctions (Cell biology); Cell interaction; Cell junction; Cell adhesion; Microcirculation
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APA (6th Edition):
Qureshi, A. (2012). The novel function of sJAM-C in promoting cytoskeleton rearrangement and migration in mammary epithelial cells. (Masters Thesis). Florida Atlantic University. Retrieved from http://purl.flvc.org/fcla/dt/3362046
Chicago Manual of Style (16th Edition):
Qureshi, Anila. “The novel function of sJAM-C in promoting cytoskeleton rearrangement and migration in mammary epithelial cells.” 2012. Masters Thesis, Florida Atlantic University. Accessed January 21, 2021.
http://purl.flvc.org/fcla/dt/3362046.
MLA Handbook (7th Edition):
Qureshi, Anila. “The novel function of sJAM-C in promoting cytoskeleton rearrangement and migration in mammary epithelial cells.” 2012. Web. 21 Jan 2021.
Vancouver:
Qureshi A. The novel function of sJAM-C in promoting cytoskeleton rearrangement and migration in mammary epithelial cells. [Internet] [Masters thesis]. Florida Atlantic University; 2012. [cited 2021 Jan 21].
Available from: http://purl.flvc.org/fcla/dt/3362046.
Council of Science Editors:
Qureshi A. The novel function of sJAM-C in promoting cytoskeleton rearrangement and migration in mammary epithelial cells. [Masters Thesis]. Florida Atlantic University; 2012. Available from: http://purl.flvc.org/fcla/dt/3362046
3.
Li, Ye.
Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth.
Degree: Doctoral Thesis, SDI, 2015, Abertay University
URL: https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05
► A complex system is a collection of parts, that can be identical or different, that interact with each other and environment, and exhibit emergent behaviour.…
(more)
▼ A complex system is a collection of parts, that can be identical or different, that interact with each other and environment, and exhibit emergent behaviour. Here, I consider the formation of vascular structures in the body as a complex system consisting of an emergent pattern in interacting endothelial cells. A cancer tumour is a different but related complex system that contains various types of cells, some of which having cancer-inducing mutations. To understand the formation of a vascular structure or a cancer tumour, it is important to understand both the single cells and cell-cell interactions. To study the physical interaction among cells in vascular formation or cancer cell growth, in this thesis an agent-based model is built based on the physical properties of cells which includes the size, shape, direction, and position of cells. In this way the mathematical equations in the model can show the physical variation among modelled cells. The 3-dimensional shape of cells is modelled, and so while I start with cell interactions in petri-dish the model can be easily extended to describe motility of cells in a 3-dimensional system in the future. The physical model is implemented and then simulated with in silico experiments, and then the spatial distribution of cells in in vitro experiments is analysed and used to calibrate the model. In vitro experiments with and without a drug in normal and hypoxic conditions are carried out. Also the patterns formed by cells with different treatment are analysed to produce different parameter combinations in the model. This physical model is shown to be able to predict vessel formation and be reused to predict the spatial distribution of cancer cells in in vitro growth experiments. With biological data such as cell size, cell shape, etc. this model is able to predict behaviours of various cell types, and can also be used to predict more complex phenomena, such as mixed type of cancer cells growing in 3-dimensions with vascular structures
Subjects/Keywords: Agent-based; Cell-cell interaction; Vascular; Cancer
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APA ·
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APA (6th Edition):
Li, Y. (2015). Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth. (Thesis). Abertay University. Retrieved from https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Ye. “Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth.” 2015. Thesis, Abertay University. Accessed January 21, 2021.
https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Ye. “Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth.” 2015. Web. 21 Jan 2021.
Vancouver:
Li Y. Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth. [Internet] [Thesis]. Abertay University; 2015. [cited 2021 Jan 21].
Available from: https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li Y. Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth. [Thesis]. Abertay University; 2015. Available from: https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Clemson University
4.
Ma, Zhen.
Electrical Coupling Between Micropatterned Cardiomyocytes and Stem Cells.
Degree: PhD, Bioengineering, 2011, Clemson University
URL: https://tigerprints.clemson.edu/all_dissertations/865
► To understand how stem cells functionally couple with native cardiomyocytes is crucial for cell-based therapies to restore the loss of cardiomyocytes that occurs during heart…
(more)
▼ To understand how stem cells functionally couple with native cardiomyocytes is crucial for
cell-based therapies to restore the loss of cardiomyocytes that occurs during heart infarction and other cardiac diseases. Due to the complexity of the in vivo environment, our knowledge of
cell coupling is heavily dependent on
cell-culture models. However, conventional in vitro studies involve undefined
cell shapes and random length of
cell-
cell contacts in addition to the presence of multiple homotypic and heterotypic contacts between interacting cells. Thus, it has not been feasible to study electrical coupling corresponding to isolated specific types of
cell contact modes. To address this issue, we used microfabrication techniques to develop different geometrically-defined stem
cell-cardiomyocyte contact assays to comparatively and quantitatively study functional stem
cell-cardiomyocyte electrical coupling. Through geometric confinements, we will construct a matrix of identical microwells, and each was constructed as a specific microenvironment. Using laser-guided
cell micropatterning technique, individual stem cells or cardiomyocytes can be deposited into the microwells to form certain contact mode. In this research, we firstly constructed an in-vivo like cardiac muscle fiber microenvironment, and the electrical conductivity of stem cells was investigated by inserting stem cells as cellular bridges. Then, the electrical coupling between cardiomyocytes and stem cells was studied at single-
cell level by constructing contact-promotive/-preventive microenvironments.
Advisors/Committee Members: Gao, Bruce Zhi, Borg , Thomas Keith, Simionescu , Dan, Dean , Delphine.
Subjects/Keywords: Cell-Cell Interaction; Cell-Microenvironment Interaction; Cell Micropatterning; Laser Guidance Technique; Biomedical Engineering and Bioengineering
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APA ·
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Manager
APA (6th Edition):
Ma, Z. (2011). Electrical Coupling Between Micropatterned Cardiomyocytes and Stem Cells. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/865
Chicago Manual of Style (16th Edition):
Ma, Zhen. “Electrical Coupling Between Micropatterned Cardiomyocytes and Stem Cells.” 2011. Doctoral Dissertation, Clemson University. Accessed January 21, 2021.
https://tigerprints.clemson.edu/all_dissertations/865.
MLA Handbook (7th Edition):
Ma, Zhen. “Electrical Coupling Between Micropatterned Cardiomyocytes and Stem Cells.” 2011. Web. 21 Jan 2021.
Vancouver:
Ma Z. Electrical Coupling Between Micropatterned Cardiomyocytes and Stem Cells. [Internet] [Doctoral dissertation]. Clemson University; 2011. [cited 2021 Jan 21].
Available from: https://tigerprints.clemson.edu/all_dissertations/865.
Council of Science Editors:
Ma Z. Electrical Coupling Between Micropatterned Cardiomyocytes and Stem Cells. [Doctoral Dissertation]. Clemson University; 2011. Available from: https://tigerprints.clemson.edu/all_dissertations/865
University of North Carolina – Greensboro
5.
Covell, Alan D.
At the interface: biotic-abiotic interactions between
substrates and a model epithelium.
Degree: 2015, University of North Carolina – Greensboro
URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887
► The need for determining the fundamental mechanisms that define the interaction of biological systems with underlying materials, both natural and synthetic, is important as humanity…
(more)
▼ The need for determining the fundamental mechanisms
that define the
interaction of biological systems with underlying
materials, both natural and synthetic, is important as humanity
endeavors to improve the quality of life of individuals through
technology. Recently, much work has focused on the role of material
properties on the behavior of cells. Most of these studies have
concentrated their efforts on fibroblastic
cell lines and more
recently different kinds of stems cells. While these cells
represent an important subset of cells in complex organisms, they
do not manifest
cell-
cell interactions, a feature of epithelial
cells, the most abundant
cell type. Epithelial cells represent the
largest
cell type in the body and introduce an intrinsic complexity
when researching the
interaction of biological systems with
materials. Adherens junctions (AJ) play a significant role in many
signaling pathways, and therefore there is need to investigate how
physical interactions with underlying substrates affect
cell-
cell
interactions, such as the adhesion properties between cells, as
well as how
cell-substrate interactions influence the morphology
and growth of epithelial cells. In this work I seek to determine
the effects and identify mechanisms that epithelial cells use to
“read” their environment. To do this I examined changes in
cell
behavior (growth, morphological, adhesion) of a model epithelium on
substrates that have similar composition but significant
differences in surface organization. In such a manner, I probed the
limitations at which the nanoscale differences in substrate
topography affect cellular behavior.; Epithelial
Cell, MDCK,
Nanotopography
Advisors/Committee Members: Dennis LaJeunesse (advisor).
Subjects/Keywords: Epithelial cells; Cell interaction; Nanotechnology
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Covell, A. D. (2015). At the interface: biotic-abiotic interactions between
substrates and a model epithelium. (Doctoral Dissertation). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887
Chicago Manual of Style (16th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between
substrates and a model epithelium.” 2015. Doctoral Dissertation, University of North Carolina – Greensboro. Accessed January 21, 2021.
http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887.
MLA Handbook (7th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between
substrates and a model epithelium.” 2015. Web. 21 Jan 2021.
Vancouver:
Covell AD. At the interface: biotic-abiotic interactions between
substrates and a model epithelium. [Internet] [Doctoral dissertation]. University of North Carolina – Greensboro; 2015. [cited 2021 Jan 21].
Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887.
Council of Science Editors:
Covell AD. At the interface: biotic-abiotic interactions between
substrates and a model epithelium. [Doctoral Dissertation]. University of North Carolina – Greensboro; 2015. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=18887
Hong Kong University of Science and Technology
6.
Ji, Tiantian LIFS.
Investigation of the mechanism underlying cell competition.
Degree: 2016, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-100399
;
https://doi.org/10.14711/thesis-b1626247
;
http://repository.ust.hk/ir/bitstream/1783.1-100399/1/th_redirect.html
► Cell competition is a phenomenon in which potentially dangerous or suboptimal cells are eliminated when they are surrounded by healthy cells. Cell competition was first…
(more)
▼ Cell competition is a phenomenon in which potentially dangerous or suboptimal cells are eliminated when they are surrounded by healthy cells. Cell competition was first discovered and best-characterized in Drosophila, a genetic system in which mosaic cell clones of varying fitness can be generated to trigger cell competition. The Drosophila neoplastic tumor suppressor mutant cells are eliminated through cell competition when they are generated as clonal cells surrounded by wild type cells. Using this system, our lab performed genetic screens and identified candidate genes important for regulating cell competition. The cell competition phenomenon has recently been observed in mammala, I would like to test whether the molecular pathways regulating cell competition in Drosophila are conserved in mammalian systems. My thesis work is to establish a cell competition system using mammalian epithelial cell lines. Key words: cell competition genetic screening molecular mechanism mammalian system
Subjects/Keywords: Cell interaction
; Cells
; Molecular genetics
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APA ·
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MLA ·
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Export
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Manager
APA (6th Edition):
Ji, T. L. (2016). Investigation of the mechanism underlying cell competition. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-100399 ; https://doi.org/10.14711/thesis-b1626247 ; http://repository.ust.hk/ir/bitstream/1783.1-100399/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ji, Tiantian LIFS. “Investigation of the mechanism underlying cell competition.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed January 21, 2021.
http://repository.ust.hk/ir/Record/1783.1-100399 ; https://doi.org/10.14711/thesis-b1626247 ; http://repository.ust.hk/ir/bitstream/1783.1-100399/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ji, Tiantian LIFS. “Investigation of the mechanism underlying cell competition.” 2016. Web. 21 Jan 2021.
Vancouver:
Ji TL. Investigation of the mechanism underlying cell competition. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Jan 21].
Available from: http://repository.ust.hk/ir/Record/1783.1-100399 ; https://doi.org/10.14711/thesis-b1626247 ; http://repository.ust.hk/ir/bitstream/1783.1-100399/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ji TL. Investigation of the mechanism underlying cell competition. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-100399 ; https://doi.org/10.14711/thesis-b1626247 ; http://repository.ust.hk/ir/bitstream/1783.1-100399/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
Covell, Alan D.
At the interface: biotic-abiotic interactions between substrates and a model epithelium.
Degree: 2015, NC Docks
URL: http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf
► The need for determining the fundamental mechanisms that define the interaction of biological systems with underlying materials, both natural and synthetic, is important as humanity…
(more)
▼ The need for determining the fundamental mechanisms that define the interaction of biological systems with underlying materials, both natural and synthetic, is important as humanity endeavors to improve the quality of life of individuals through technology. Recently, much work has focused on the role of material properties on the behavior of cells. Most of these studies have concentrated their efforts on fibroblastic cell lines and more recently different kinds of stems cells. While these cells represent an important subset of cells in complex organisms, they do not manifest cell-cell interactions, a feature of epithelial cells, the most abundant cell type. Epithelial cells represent the largest cell type in the body and introduce an intrinsic complexity when researching the interaction of biological systems with materials. Adherens junctions (AJ) play a significant role in many signaling pathways, and therefore there is need to investigate how physical interactions with underlying substrates affect cell-cell interactions, such as the adhesion properties between cells, as well as how cell-substrate interactions influence the morphology and growth of epithelial cells. In this work I seek to determine the effects and identify mechanisms that epithelial cells use to “read” their environment. To do this I examined changes in cell behavior (growth, morphological, adhesion) of a model epithelium on substrates that have similar composition but significant differences in surface organization. In such a manner, I probed the limitations at which the nanoscale differences in substrate topography affect cellular behavior.
Subjects/Keywords: Epithelial cells; Cell interaction; Nanotechnology
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Covell, A. D. (2015). At the interface: biotic-abiotic interactions between substrates and a model epithelium. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between substrates and a model epithelium.” 2015. Thesis, NC Docks. Accessed January 21, 2021.
http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Covell, Alan D. “At the interface: biotic-abiotic interactions between substrates and a model epithelium.” 2015. Web. 21 Jan 2021.
Vancouver:
Covell AD. At the interface: biotic-abiotic interactions between substrates and a model epithelium. [Internet] [Thesis]. NC Docks; 2015. [cited 2021 Jan 21].
Available from: http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Covell AD. At the interface: biotic-abiotic interactions between substrates and a model epithelium. [Thesis]. NC Docks; 2015. Available from: http://libres.uncg.edu/ir/uncg/f/Covell_uncg_0154D_11788.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
8.
Guo, Feng.
Acoustic tweezers: manipulating micro-objects with the power of sound
.
Degree: 2015, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/27341
► Sound can be music to please the ear, however the waves produced can be utilized as “Acoustic Tweezers” for the manipulation of cells and particles…
(more)
▼ Sound can be music to please the ear, however the waves produced can be utilized as “Acoustic Tweezers” for the manipulation of cells and particles in a fluid medium.
The ability to dexterously and noninvasively manipulate biological specimens such as organisms, cells, proteins, and DNAs in a compact system is critical for many applications in the fields of life sciences, biomedicine and chemistry. Acoustic tweezer technology is a revolutionary way to satisfy this requirement. Firstly, this technique manipulates cells or particles using gentle mechanical vibrations. These vibrations create a pressure gradient in the medium to move suspended micro-objects yielding a contamination-free, non-contact, and label-free manipulation. Secondly, acoustic tweezers have minimal impact on
cell viability and function, which operates at a power intensity and frequency similar to the widely used medical ultrasound imaging. Thirdly, the acoustic tweezer device can operate in a single micro-device without any external moving parts or complicate setups, which offer additional advantages in ease of use, versatility and portability.
In this dissertation, we have developed a series of acoustic tweezers that can achieve manipulation of micro-objects in a liquid medium: 1) tunable acoustic wells to control
cell-
cell distance and geometry of suspended
cell assemblies; 2) 3D acoustic tweezers to dexterously transport single cells in a three-dimensional manner; 3) simple, low-cost and reusable acoustic tweezers used for various disposable devices; and 4) application of the reusable acoustic tweezer technology in precisely manipulating and patterning micrometer-sized protein crystals for X-ray crystallography.
Advisors/Committee Members: Jun Huang, Dissertation Advisor/Co-Advisor, Jun Huang, Committee Chair/Co-Chair, Bernhard R Tittmann, Committee Member, Bruce Gluckman, Committee Member, Corina Stefania Drapaca, Committee Member, Siyang Zheng, Special Member.
Subjects/Keywords: acoustic tweezers; microfluidics; cell-cell interaction; cell printing; crystallography; disposable device.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Guo, F. (2015). Acoustic tweezers: manipulating micro-objects with the power of sound
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/27341
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Guo, Feng. “Acoustic tweezers: manipulating micro-objects with the power of sound
.” 2015. Thesis, Penn State University. Accessed January 21, 2021.
https://submit-etda.libraries.psu.edu/catalog/27341.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Guo, Feng. “Acoustic tweezers: manipulating micro-objects with the power of sound
.” 2015. Web. 21 Jan 2021.
Vancouver:
Guo F. Acoustic tweezers: manipulating micro-objects with the power of sound
. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 21].
Available from: https://submit-etda.libraries.psu.edu/catalog/27341.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Guo F. Acoustic tweezers: manipulating micro-objects with the power of sound
. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/27341
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cincinnati
9.
Taghian, Toloo.
Interaction of an Electric Field with Vascular Cells.
Degree: PhD, Arts and Sciences: Physics, 2015, University of Cincinnati
URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439309071
► Development of effective treatments for impaired healing of vascular tissues is essential to address significant health care problems associated with these conditions, including chronic ulcers.…
(more)
▼ Development of effective treatments for impaired
healing of vascular tissues is essential to address significant
health care problems associated with these conditions, including
chronic ulcers. Clinical studies reveal that an electric field has
the potential as a novel vascular therapy to accelerate healing of
hard-to-heal wounds. However, widespread acceptance of electric
field therapies for wound healing has been prevented by a lack of
standardized protocols, leading to variabilities in healing
outcomes. External electric fields can affect a variety of vascular
cell responses through the manipulation of the native electric
field in the extracellular ionic environment and across the
cell
membrane. Therefore, an electric field, together with the
extracellular matrix and a milieu of cytokines, represents a
biophysical system that ultimately regulates vascular
cell
function. Therapeutic modulation of this system requires advanced
integration of knowledge and technology from both physics and
biomedical sciences. The long term goal of this research is to
create non-pharmacological, electric field-based therapies for non-
or slow-healing chronic ulcers. The studies in this research
contribute to this goal by developing a theoretical-experimental
approach to elucidate the biophysical mechanisms of
cell- electric
field interactions mediated by the extracellular matrix. The
numerical model of
cell-electric field
interaction determines the
distribution of the induced electric field in the
cell and
extracellular environment. The novelty of the model is that it
considers the
cell attached to a substrate to represent cells
within tissues. The results show a striking difference in the (1)
frequency dependence of electric field penetration and (2)
cell
response between cells suspended in an electrolyte and cells
attached to a substrate. The results demonstrate that, at low
frequency, electric field is confined in the
cell membrane and is
expected to regulate membrane-initiated responses. At high
frequency, electric field penetrates the
cell and may directly
activate intracellular responses .Importantly, the sensitive
dependence of electric field distribution in the
cell to the
physical properties of the
cell and its environment is
demonstrated. Additionally, the advantage of non- contact electric
field application for research and therapeutic purposes is
discussed.The experimental studies of cell– electric field
interaction confirm the theoretical predictions and show the
frequency- specific
cell responses and demonstrate the
intracellular pathway activation in response to high frequency
electric field when electric field is induced in the intracellular
space. The results show that extracellular matrices with different
matrix properties can differentially regulate protein expression in
response to the high frequency electric field, confirming the
importance of incorporating substrate properties to study
cell-field interactions. Importantly,
cell responses that promote
wound healing process are activated following electrical…
Advisors/Committee Members: Kogan, Andrei (Committee Chair).
Subjects/Keywords: Biophysics; electrical cell stimulation; cell transmembrane potential; frequency-dependent response; surface charge; cell-substrate interaction
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APA (6th Edition):
Taghian, T. (2015). Interaction of an Electric Field with Vascular Cells. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439309071
Chicago Manual of Style (16th Edition):
Taghian, Toloo. “Interaction of an Electric Field with Vascular Cells.” 2015. Doctoral Dissertation, University of Cincinnati. Accessed January 21, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439309071.
MLA Handbook (7th Edition):
Taghian, Toloo. “Interaction of an Electric Field with Vascular Cells.” 2015. Web. 21 Jan 2021.
Vancouver:
Taghian T. Interaction of an Electric Field with Vascular Cells. [Internet] [Doctoral dissertation]. University of Cincinnati; 2015. [cited 2021 Jan 21].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439309071.
Council of Science Editors:
Taghian T. Interaction of an Electric Field with Vascular Cells. [Doctoral Dissertation]. University of Cincinnati; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439309071
Ryerson University
10.
Hughes, Bethany R.
Development of a tunable 3D wrinkled platfom for designing cellular three-dimensional communication networks.
Degree: 2016, Ryerson University
URL: https://digital.library.ryerson.ca/islandora/object/RULA%3A6587
► The study of cell-cell communication is hindered by the absence of a platform which is capable of specifically directing cellular growth while allowing examination of…
(more)
▼ The study of cell-cell communication is hindered by the absence of a platform which is capable of specifically directing cellular growth while allowing examination of the communication between cells. In this thesis, a tuneable micro-to-nano scale wrinkled nonplanar platform was developed and optimized through the use of photolithography and a microfluidic washing method. The platform demonstrated the ability to create micro and nanowrinkled structures in a wide range of flow conditions.
The developed platform was then used as a cell culture platform to investigate the spacing dependence of bovine fibroblasts. The identification of a critical bridging distance for bovine fibroblasts provided a means to optimized the platform for culturing a cellular communication network between bovine fibroblasts. The cellular network which resulted demonstrated, via FRAP (Fluorescence-recovery-after-photobleaching), the capacity for communication between cells. Creating multilevel length scaled structures on a tunable platform which directed cellular growth while maintaining communication presents potential applications in research, industry and clinical aspects.
Subjects/Keywords: Cell adhesion; Cell interaction; Stem cells; Fibroblasts; Tissue engineering
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APA (6th Edition):
Hughes, B. R. (2016). Development of a tunable 3D wrinkled platfom for designing cellular three-dimensional communication networks. (Thesis). Ryerson University. Retrieved from https://digital.library.ryerson.ca/islandora/object/RULA%3A6587
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hughes, Bethany R. “Development of a tunable 3D wrinkled platfom for designing cellular three-dimensional communication networks.” 2016. Thesis, Ryerson University. Accessed January 21, 2021.
https://digital.library.ryerson.ca/islandora/object/RULA%3A6587.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hughes, Bethany R. “Development of a tunable 3D wrinkled platfom for designing cellular three-dimensional communication networks.” 2016. Web. 21 Jan 2021.
Vancouver:
Hughes BR. Development of a tunable 3D wrinkled platfom for designing cellular three-dimensional communication networks. [Internet] [Thesis]. Ryerson University; 2016. [cited 2021 Jan 21].
Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A6587.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hughes BR. Development of a tunable 3D wrinkled platfom for designing cellular three-dimensional communication networks. [Thesis]. Ryerson University; 2016. Available from: https://digital.library.ryerson.ca/islandora/object/RULA%3A6587
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Alberta
11.
Renaud-Young, Margaret.
Structure function study of L-CAM: insight into the role of
the first extracellular domain in the trans cell-cell
interaction.
Degree: MS, Department of Biological Sciences, 2001, University of Alberta
URL: https://era.library.ualberta.ca/files/t435gg53f
Subjects/Keywords: Cell adhesion.; Cell interaction.
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APA (6th Edition):
Renaud-Young, M. (2001). Structure function study of L-CAM: insight into the role of
the first extracellular domain in the trans cell-cell
interaction. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/t435gg53f
Chicago Manual of Style (16th Edition):
Renaud-Young, Margaret. “Structure function study of L-CAM: insight into the role of
the first extracellular domain in the trans cell-cell
interaction.” 2001. Masters Thesis, University of Alberta. Accessed January 21, 2021.
https://era.library.ualberta.ca/files/t435gg53f.
MLA Handbook (7th Edition):
Renaud-Young, Margaret. “Structure function study of L-CAM: insight into the role of
the first extracellular domain in the trans cell-cell
interaction.” 2001. Web. 21 Jan 2021.
Vancouver:
Renaud-Young M. Structure function study of L-CAM: insight into the role of
the first extracellular domain in the trans cell-cell
interaction. [Internet] [Masters thesis]. University of Alberta; 2001. [cited 2021 Jan 21].
Available from: https://era.library.ualberta.ca/files/t435gg53f.
Council of Science Editors:
Renaud-Young M. Structure function study of L-CAM: insight into the role of
the first extracellular domain in the trans cell-cell
interaction. [Masters Thesis]. University of Alberta; 2001. Available from: https://era.library.ualberta.ca/files/t435gg53f
Stellenbosch University
12.
Luyt, Natasha Alethea.
Interaction of multiple yeast species during fermentation.
Degree: MSc, Viticulture and Oenology, 2015, Stellenbosch University
URL: http://hdl.handle.net/10019.1/97013
► ENGLISH ABSTRACT: The use of non-Saccharomyces yeasts together with the yeast S. cerevisiae in multistarter wine fermentations has emerged as a useful tool to modulate…
(more)
▼ ENGLISH ABSTRACT: The use of non-Saccharomyces yeasts together with the yeast S. cerevisiae in multistarter wine fermentations has emerged as a useful tool to modulate wine aroma and/or to decrease the concentration of undesirable compounds. However, upon inoculation, these yeast species do not co-exist passively, but interact in various ways. While competition for nutrients and the excretion of killer toxins in an antagonistic relationship are obvious and well established types of interactions, some studies have suggested the existence of other forms of cellular or molecular interactions. One of these includes physical
cell-
cell contact and to our knowledge, only one previous study has confirmed its existence in wine yeasts. Yeast interactions are also influenced by other factors, such as ethanol concentration, however some studies have highlighted the role that dissolved oxygen plays on the survival of non-Saccharomyces yeasts and their ability to compete for space with S. cerevisiae and little research has focused on this.
This study aimed to investigate the occurrence of a physical
cell-
cell and/or metabolic
interaction between S. cerevisiae and L. thermotolerans in mixed culture fermentations of synthetic grape must. For this purpose, fermentations in a Double Compartment Bioreactor (DCB) which separates yeast population through the use of a membrane were compared to mixed fermentations in the absence of the membrane, using the same reactor. Furthermore, the impact of oxygen supply on yeast behaviour was also assessed.
Following mixed culture fermentations in a DCB, it was observed that the presence of S. cerevisiae led to a significant decline in viability in L. thermotolerans. This decline was significantly less prominent in mixed cultures where the cells were in indirect contact. Together, the data provided evidence for both
cell-
cell and metabolic interactions whereby S. cerevisiae had a strong negative influence on the growth of L. thermotolerans. However, it was also observed that L. thermotolerans had some negative impact on the growth of S. cerevisiae, leading to a reduction in biomass (when in indirect contact) and a reduced maximum CFU/mL compared to pure cultures. The data also suggest that direct physical contact may increase the production of glycerol and propanol, but this needs further investigation.
By decreasing the frequency at which oxygen pulses were provided, a reduction in biomass and increase in fermentation duration was observed for all fermentations. However, this effect was somewhat reduced in mixed cultures. Here, no impact on fermentation duration was observed and the decrease in biomass was less compared to pure cultures. The impact of these oxygen pulses was also greater on L. thermotolerans. In the latter yeast’s pure culture a slight increase in glycerol was observed when less oxygen was provided and in general there appeared to be no impact on acetic acid production. Furthermore, there was little or no impact on volatile production, however, more repeats might reveal different…
Advisors/Committee Members: Bauer, Florian F., Divol, Benoit, Stellenbosch University. Faculty of Agrisciences. Dept. of Viticulture and Oenology. Institute for Wine Biotechnology..
Subjects/Keywords: Double compartment bioreactor; Cell-cell interaction; Mixed culture fermentation; UCTD
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Manager
APA (6th Edition):
Luyt, N. A. (2015). Interaction of multiple yeast species during fermentation. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/97013
Chicago Manual of Style (16th Edition):
Luyt, Natasha Alethea. “Interaction of multiple yeast species during fermentation.” 2015. Masters Thesis, Stellenbosch University. Accessed January 21, 2021.
http://hdl.handle.net/10019.1/97013.
MLA Handbook (7th Edition):
Luyt, Natasha Alethea. “Interaction of multiple yeast species during fermentation.” 2015. Web. 21 Jan 2021.
Vancouver:
Luyt NA. Interaction of multiple yeast species during fermentation. [Internet] [Masters thesis]. Stellenbosch University; 2015. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10019.1/97013.
Council of Science Editors:
Luyt NA. Interaction of multiple yeast species during fermentation. [Masters Thesis]. Stellenbosch University; 2015. Available from: http://hdl.handle.net/10019.1/97013
13.
Li, Ye.
Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth.
Degree: PhD, 2015, Abertay University
URL: https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678803
► A complex system is a collection of parts, that can be identical or different, that interact with each other and environment, and exhibit emergent behaviour.…
(more)
▼ A complex system is a collection of parts, that can be identical or different, that interact with each other and environment, and exhibit emergent behaviour. Here, I consider the formation of vascular structures in the body as a complex system consisting of an emergent pattern in interacting endothelial cells. A cancer tumour is a different but related complex system that contains various types of cells, some of which having cancer-inducing mutations. To understand the formation of a vascular structure or a cancer tumour, it is important to understand both the single cells and cell-cell interactions. To study the physical interaction among cells in vascular formation or cancer cell growth, in this thesis an agent-based model is built based on the physical properties of cells which includes the size, shape, direction, and position of cells. In this way the mathematical equations in the model can show the physical variation among modelled cells. The 3-dimensional shape of cells is modelled, and so while I start with cell interactions in petri-dish the model can be easily extended to describe motility of cells in a 3-dimensional system in the future. The physical model is implemented and then simulated with in silico experiments, and then the spatial distribution of cells in in vitro experiments is analysed and used to calibrate the model. In vitro experiments with and without a drug in normal and hypoxic conditions are carried out. Also the patterns formed by cells with different treatment are analysed to produce different parameter combinations in the model. This physical model is shown to be able to predict vessel formation and be reused to predict the spatial distribution of cancer cells in in vitro growth experiments. With biological data such as cell size, cell shape, etc. this model is able to predict behaviours of various cell types, and can also be used to predict more complex phenomena, such as mixed type of cancer cells growing in 3-dimensions with vascular structures.
Subjects/Keywords: 616.99; Agent-based; Cell-cell interaction; Vascular; Cancer
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to Zotero / EndNote / Reference
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APA (6th Edition):
Li, Y. (2015). Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth. (Doctoral Dissertation). Abertay University. Retrieved from https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678803
Chicago Manual of Style (16th Edition):
Li, Ye. “Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth.” 2015. Doctoral Dissertation, Abertay University. Accessed January 21, 2021.
https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678803.
MLA Handbook (7th Edition):
Li, Ye. “Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth.” 2015. Web. 21 Jan 2021.
Vancouver:
Li Y. Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth. [Internet] [Doctoral dissertation]. Abertay University; 2015. [cited 2021 Jan 21].
Available from: https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678803.
Council of Science Editors:
Li Y. Agent-based modelling of cell-cell interactions for in vitro vascular formation and cancer cell growth. [Doctoral Dissertation]. Abertay University; 2015. Available from: https://rke.abertay.ac.uk/en/studentTheses/4e989b30-daa6-4aa9-8f06-87cf49737a05 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678803
Michigan State University
14.
Hsu, Hong.
Inhibition of intercellular communication in rat leydig cells in vitro by xenobiotic chemicals.
Degree: MS, Medical Technology Program, 1991, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:23184
Subjects/Keywords: Cell interaction; Cell junctions; Xenobiotics
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APA (6th Edition):
Hsu, H. (1991). Inhibition of intercellular communication in rat leydig cells in vitro by xenobiotic chemicals. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:23184
Chicago Manual of Style (16th Edition):
Hsu, Hong. “Inhibition of intercellular communication in rat leydig cells in vitro by xenobiotic chemicals.” 1991. Masters Thesis, Michigan State University. Accessed January 21, 2021.
http://etd.lib.msu.edu/islandora/object/etd:23184.
MLA Handbook (7th Edition):
Hsu, Hong. “Inhibition of intercellular communication in rat leydig cells in vitro by xenobiotic chemicals.” 1991. Web. 21 Jan 2021.
Vancouver:
Hsu H. Inhibition of intercellular communication in rat leydig cells in vitro by xenobiotic chemicals. [Internet] [Masters thesis]. Michigan State University; 1991. [cited 2021 Jan 21].
Available from: http://etd.lib.msu.edu/islandora/object/etd:23184.
Council of Science Editors:
Hsu H. Inhibition of intercellular communication in rat leydig cells in vitro by xenobiotic chemicals. [Masters Thesis]. Michigan State University; 1991. Available from: http://etd.lib.msu.edu/islandora/object/etd:23184
Virginia Tech
15.
Kargar, Mehdi.
Controlling Microbial Colonization and Biofilm Formation Using Topographical Cues.
Degree: PhD, Mechanical Engineering, 2015, Virginia Tech
URL: http://hdl.handle.net/10919/71741
► This dissertation introduces assembly of spherical particles as a novel topography-based anti-biofouling coating. It also provides new insights on the effects of surface topography, especially…
(more)
▼ This dissertation introduces assembly of spherical particles as a novel topography-based anti-biofouling coating. It also provides new insights on the effects of surface topography, especially local curvature, on cell–surface and cell–
cell interactions during the evolution of biofilms.
I investigated the adhesion, colonization, and biofilm formation of the opportunistic human pathogen Pseudomonas aeruginosa on a solid coated in close-packed spheres of polystyrene, using flat polystyrene sheets as a control. The results show that, whereas flat sheets are covered in large clusters after one day, a close-packed layer of 630–1550 nm monodisperse spheres prevents cluster formation. Moreover, the film of spheres reduces the density of P. aeruginosa adhered to the solid by 80%. Our data show that when P. aeruginosa adheres to the spheres, the distribution is not random. For 630 nm and larger particles, P. aeruginosa tends to position its body in the confined spaces between particles. After two days, 3D biofilm structures cover much of the flat polystyrene, whereas 3D biofilms rarely occur on a solid with a colloidal crystal coating of 1550 nm spheres. On 450 nm colloidal crystals, the bacterial growth was intermediate between the flat and 1550 nm spheres. The initial preference for P. aeruginosa to adhere to confined spaces is maintained on the second day, even when the cells form clusters: the cells remain in the confined spaces to form non-touching clusters. When the cells do touch, the contact is usually the pole, not the sides of the bacteria. The observations are rationalized based on the potential gains and costs associated with
cell-sphere and
cell-
cell contacts. I concluded that the anti-biofilm property of the colloidal crystals is correlated with the ability to arrange the individual cells.
I showed that a colloidal crystal coating delays P. aeruginosa cluster formation on a medical-grade stainless-steel needle. This suggests that a colloidal crystal approach to biofilm inhibition might be applicable to other materials and geometries. The results presented in appendix 1 suggest that colloidal crystals can also delay adhesion of Methicillin resistant staphylococcus aureus (MRSA) while it supports selective adhesion of this bacterium to the confined spaces.
Advisors/Committee Members: Ducker, William A. (committeechair), Agah, Masoud (committee member), Davalos, Rafael V. (committee member), Pruden, Amy (committee member).
Subjects/Keywords: Pseudomonas aeruginosa; Colloidal Crystals; Anti-Fouling Coating; Curvature; Cell-Cell interaction
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APA (6th Edition):
Kargar, M. (2015). Controlling Microbial Colonization and Biofilm Formation Using Topographical Cues. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/71741
Chicago Manual of Style (16th Edition):
Kargar, Mehdi. “Controlling Microbial Colonization and Biofilm Formation Using Topographical Cues.” 2015. Doctoral Dissertation, Virginia Tech. Accessed January 21, 2021.
http://hdl.handle.net/10919/71741.
MLA Handbook (7th Edition):
Kargar, Mehdi. “Controlling Microbial Colonization and Biofilm Formation Using Topographical Cues.” 2015. Web. 21 Jan 2021.
Vancouver:
Kargar M. Controlling Microbial Colonization and Biofilm Formation Using Topographical Cues. [Internet] [Doctoral dissertation]. Virginia Tech; 2015. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10919/71741.
Council of Science Editors:
Kargar M. Controlling Microbial Colonization and Biofilm Formation Using Topographical Cues. [Doctoral Dissertation]. Virginia Tech; 2015. Available from: http://hdl.handle.net/10919/71741
Rutgers University
16.
Caicedo-Carvajal, Carlos Eduardo, 1976.
In vitro and in silico findings on cell-cell and cell-ECM interactions during cellular aggregation and rearrangement:.
Degree: PhD, Biomedical Engineering, 2009, Rutgers University
URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051787
► In this dissertation, we present in silico and in vitro work on the dynamics of cellular aggregation and rearrangement as cell-cell and cell-ECM interactions are…
(more)
▼ In this dissertation, we present in silico and in vitro work on the dynamics of cellular aggregation and rearrangement as cell-cell and cell-ECM interactions are systematically varied. Computationally, we explore the contributions of homotypic and heterotypic forces between cells and the ECM affect cellular self-assembly. We find that variation of homotypic and heterotypic forces generates both expected morphologies and previously unreported patterns. Among the newly discovered patterns are segmented states of alternating cell types, and an “onion” state, in which cells form multilayer-aggregates of two cell types. Experimentally we varied cell-ECM adhesive strength through selection of α5β1-integrin receptor expression in Chinese Hamster Ovary (CHO) Cells at two soluble fibronectin (sFn) concentrations. Second, to describe dual adhesive relations, we used a CHO cell line variants coexpressing integrin and N-cadherin surface receptors. We found previously unreported complex behaviors of aggregates in these experiments. For example, we found that at constant sFn concentration, aggregate cohesion grows linearly as α5β1 receptor density is increased from low to moderate levels. However, further increase in receptor expression causes an abrupt drop in tissue cohesion. We propose that the observed biphasic property of these aggregates may be due to depletion of sFn below a critical value in the aggregate microenvironment at high α5β1 expression level. We also found that a complicated interplay emerges when cell-ECM and cell-cell interactions mediate cellular aggregation and rearrangement. Thus, we describe two nodes of cellular interaction, cell-ECM and cell-cell/cell-ECM. For weak cell-ECM interactions, cells can still rearrange, and new cellular patterns (e.g. inverted structures) emerge. For high cell-ECM strengths, cells are bound to the matrix and cannot rearrange. For weak and high cell-ECM interactions, cell-cell governs final equilibrium configurations. We propose that these results have potential implications for embryonic development, for wound healing, and for cancer therapeutic applications.
Advisors/Committee Members: Caicedo-Carvajal, Carlos Eduardo, 1976 (author), Shinbrot, Troy (chair), Foty, Ramsey (co-chair), Roth, Charlie (internal member), Corbett, Siobhan (outside member).
Subjects/Keywords: Cell aggregation; Cell interaction
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APA (6th Edition):
Caicedo-Carvajal, Carlos Eduardo, 1. (2009). In vitro and in silico findings on cell-cell and cell-ECM interactions during cellular aggregation and rearrangement:. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051787
Chicago Manual of Style (16th Edition):
Caicedo-Carvajal, Carlos Eduardo, 1976. “In vitro and in silico findings on cell-cell and cell-ECM interactions during cellular aggregation and rearrangement:.” 2009. Doctoral Dissertation, Rutgers University. Accessed January 21, 2021.
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051787.
MLA Handbook (7th Edition):
Caicedo-Carvajal, Carlos Eduardo, 1976. “In vitro and in silico findings on cell-cell and cell-ECM interactions during cellular aggregation and rearrangement:.” 2009. Web. 21 Jan 2021.
Vancouver:
Caicedo-Carvajal, Carlos Eduardo 1. In vitro and in silico findings on cell-cell and cell-ECM interactions during cellular aggregation and rearrangement:. [Internet] [Doctoral dissertation]. Rutgers University; 2009. [cited 2021 Jan 21].
Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051787.
Council of Science Editors:
Caicedo-Carvajal, Carlos Eduardo 1. In vitro and in silico findings on cell-cell and cell-ECM interactions during cellular aggregation and rearrangement:. [Doctoral Dissertation]. Rutgers University; 2009. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051787
Portland State University
17.
Myers, Janette Bernadette.
Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes.
Degree: PhD, Chemistry, 2019, Portland State University
URL: https://pdxscholar.library.pdx.edu/open_access_etds/5008
► Gap junctions are a class of membrane proteins that facilitate cell-to-cell communication by forming channels that directly couple the cytoplasm of neighboring cells. The…
(more)
▼ Gap junctions are a class of membrane proteins that facilitate
cell-to-
cell communication by forming channels that directly couple the cytoplasm of neighboring cells. The channels are composed of monomers called connexins. Humans express 21 connexin isoforms in a
cell-type specific fashion, and each isoform has distinct mechanisms of permeation and regulation. Co-assembly of multiple isoforms into a single intercellular channel can change channel properties, such as conductance and selectivity to substrates (e.g., ions, metabolites and signaling molecules). However, the mechanistic basis for this functional diversity has remained poorly understood. This lack of mechanistic insight has been due in large part to the lack of high-resolution (atomic-level) structural knowledge on this class of proteins. Prior to this work, the only high-resolution information available on gap junction structure came from a single connexin isoform, connexin-26 (cx26).
CryoEM has recently transformed from a low-resolution technique into one capable of rivaling the atomic-level resolutions achieved by x-ray crystallography – but without the necessity for crystal formation, which has hindered progress towards understanding many classes of proteins (ie, membrane proteins, intrinsically disordered
cell signaling complexes and other structurally dynamic systems). For my thesis research, I applied novel methods in single particle electron cryo-microscopy (CryoEM) to study a class of membrane proteins called gap junctions isolated from native lens tissue, as well as two signaling complexes not amenable to other structural techniques. I determined the structure of the lens gap junction, which contains connexin-46 (cx46) and connexin-50 (cx50), to a resolution of 3.4 Å and generated atomic models for both connexin isoforms. Structural analysis paired with molecular dynamics gave insight into energetic features of these channels that determine their isoform-specific conductance and selectivity to electrically charged ions. The cx46/50 gating domain was found to be stabilized by hydrophobic anchors, and also seems to adopt a more stable open state than found in cx26. Genetic mutations associated with congenital cataract formation were found to map to hot-spots of conserved structural and functional importance, rationalizing their disease-causing effects.
As part of collaborative efforts, I used methods in single particle EM to characterize two separate signaling complexes that had proven difficult to study with x-ray crystallography and NMR spectroscopy. One system, Ca
2+/Calmodulin Kinase II (CaMKII), is a signaling complex in the brain involved in memory formation. Characterization of the CaMKII complex by single particle EM revealed an extended state, which was also shown to be prevalent in cells – giving more depth to our understanding of how this signaling molecule functions. The second collaboration characterized the multimeric binding sites of the hub protein LC8, which interacts with the disordered region of a…
Advisors/Committee Members: Steve Reichow.
Subjects/Keywords: Gap junctions (Cell biology); Cell interaction; Connexins; Biochemistry; Molecular Biology
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APA (6th Edition):
Myers, J. B. (2019). Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes. (Doctoral Dissertation). Portland State University. Retrieved from https://pdxscholar.library.pdx.edu/open_access_etds/5008
Chicago Manual of Style (16th Edition):
Myers, Janette Bernadette. “Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes.” 2019. Doctoral Dissertation, Portland State University. Accessed January 21, 2021.
https://pdxscholar.library.pdx.edu/open_access_etds/5008.
MLA Handbook (7th Edition):
Myers, Janette Bernadette. “Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes.” 2019. Web. 21 Jan 2021.
Vancouver:
Myers JB. Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes. [Internet] [Doctoral dissertation]. Portland State University; 2019. [cited 2021 Jan 21].
Available from: https://pdxscholar.library.pdx.edu/open_access_etds/5008.
Council of Science Editors:
Myers JB. Application of Single Particle Electron Microscopy to Native Lens Gap Junctions and Intrinsically Disordered Signaling Complexes. [Doctoral Dissertation]. Portland State University; 2019. Available from: https://pdxscholar.library.pdx.edu/open_access_etds/5008
Columbia University
18.
Kirkling, Margaret Elizabeth.
Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells.
Degree: 2019, Columbia University
URL: https://doi.org/10.7916/d8-fks1-e369
► Dendritic cells (DCs) comprise a heterogeneous population of mononuclear phagocytes that play a critical role in both innate and adaptive immunity. DCs in mice can…
(more)
▼ Dendritic cells (DCs) comprise a heterogeneous population of mononuclear phagocytes that play a critical role in both innate and adaptive immunity. DCs in mice can be divided into two main types. Plasmacytoid DCs (pDCs) secrete type I interferons (IFN-α/β) in response to viruses. Classical or conventional dendritic cells (cDCs) are highly adept at Ag presentation. There are two main subsets of cDCs; the CD11b+ cDC subset presents exogenous Ag to CD4+ T cells on major histocompatibility complex class II (MHCII) and the CD8α+/CD103+ cDCs uniquely capable of cross-presenting exogenous Ag to CD8+ T cells on MHCI. Functional equivalents of both subsets have been identified in humans and have been designated cDC2 and cDC1, respectively. All DCs develop from progenitors found in the bone marrow (BM) by a process directed primarily by the cytokine Fms-like tyrosine kinase 3 ligand (FL). The specification of DC types is driven by several transcription factors such as IRF8, while terminal cDC differentiation is guided by tissue-specific signals mediated through signaling pathways such as Notch and lymphotoxin-β. Notch is an evolutionarily conserved pathway of cell-cell communication that plays an essential role in the development of immune cell types, including T and B lymphocytes. DC-specific gene targeting, has been used to establish the role of Notch2 receptor signaling in the differentiation of cDC2 subset in the spleen and intestine and splenic cDC1.
Because primary cDCs, particularly cDC1, are rare in vivo their study and use in translational applications require methods to generate functional cDC subsets in vitro. Commonly used cultures of primary BM with the cytokines FL or granulocyte-monocyte colony stimulating factor (GM-CSF) produce a mixture of pDC, cDC2 and cDC1-like cells, or cDC2-like cells and macrophages, respectively. Thus, new approaches are needed to yield high numbers of fully differentiated cDCs, particularly of mature cDC1. Given the critical role of Notch signaling in cDC differentiation in vivo, I hypothesized that it would facilitate cDC differentiation in vitro. Indeed, coculture of murine primary BM cells with OP9 stromal cells expressing Notch ligand Delta-like 1 (OP9-DL1) facilitated the generation of bona fide, IRF8-dependent CD8α+ CD103+ Dec205+ cDC1 with an expression profile resembling ex vivo cDC1. Critically, the resulting cDC1 showed improved Ccr7-dependent migration, superior T cell cross-priming capacity and antitumor vaccination in vivo. Further, OP9-DL1 cocultures of immortalized progenitors allowed for the de novo generation CD8α+ cDC1.
This discovery can help further our understanding of the mechanisms of DC differentiation while providing a tool to allow for the generation of unlimited numbers of cDCs for functional studies. Further, as cDC1 are essential for the cross-priming of cytotoxic T cells against tumors, they hold great promise as cellular vaccines. However, the use of DCs in clinical applications has been hampered by inadequate methods to generate large…
Subjects/Keywords: Immunology; Dendritic cells; Cell interaction; Genetics
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MLA ·
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APA (6th Edition):
Kirkling, M. E. (2019). Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/d8-fks1-e369
Chicago Manual of Style (16th Edition):
Kirkling, Margaret Elizabeth. “Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells.” 2019. Doctoral Dissertation, Columbia University. Accessed January 21, 2021.
https://doi.org/10.7916/d8-fks1-e369.
MLA Handbook (7th Edition):
Kirkling, Margaret Elizabeth. “Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells.” 2019. Web. 21 Jan 2021.
Vancouver:
Kirkling ME. Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells. [Internet] [Doctoral dissertation]. Columbia University; 2019. [cited 2021 Jan 21].
Available from: https://doi.org/10.7916/d8-fks1-e369.
Council of Science Editors:
Kirkling ME. Notch signaling facilitates in vitro generation of cross-presenting classical dendritic cells. [Doctoral Dissertation]. Columbia University; 2019. Available from: https://doi.org/10.7916/d8-fks1-e369
19.
SUN YONGJIANG.
EARLY INTERACTIONS OF DEN-2 VIRUS AND CELLS.
Degree: 1998, National University of Singapore
URL: https://scholarbank.nus.edu.sg/handle/10635/153216
Subjects/Keywords: Viruses; Cell interaction
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APA (6th Edition):
YONGJIANG, S. (1998). EARLY INTERACTIONS OF DEN-2 VIRUS AND CELLS. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/153216
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
YONGJIANG, SUN. “EARLY INTERACTIONS OF DEN-2 VIRUS AND CELLS.” 1998. Thesis, National University of Singapore. Accessed January 21, 2021.
https://scholarbank.nus.edu.sg/handle/10635/153216.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
YONGJIANG, SUN. “EARLY INTERACTIONS OF DEN-2 VIRUS AND CELLS.” 1998. Web. 21 Jan 2021.
Vancouver:
YONGJIANG S. EARLY INTERACTIONS OF DEN-2 VIRUS AND CELLS. [Internet] [Thesis]. National University of Singapore; 1998. [cited 2021 Jan 21].
Available from: https://scholarbank.nus.edu.sg/handle/10635/153216.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
YONGJIANG S. EARLY INTERACTIONS OF DEN-2 VIRUS AND CELLS. [Thesis]. National University of Singapore; 1998. Available from: https://scholarbank.nus.edu.sg/handle/10635/153216
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Minnesota
20.
Wu, Xiaojie.
Development of Biomimetic Microfluidic Platforms for Cellular Interaction Studies.
Degree: PhD, Chemistry, 2016, University of Minnesota
URL: http://hdl.handle.net/11299/182827
► Achieving a better understanding of cellular interactions with other critical components in physiological microenvironments is an urgent challenge due to the fact that critical cellular…
(more)
▼ Achieving a better understanding of cellular interactions with other critical components in physiological microenvironments is an urgent challenge due to the fact that critical cellular behaviors are delicately regulated by the complexity of the biological system. Factors influencing cellular behaviors include interactions with the surrounding cell types and biological molecules, as well as a range of biophysical factors, such as pressure, flow, and chemical gradients. With a better understanding of environmental impacts on cellular behaviors, mechanistic insights on the pathogenesis of diseases and advances in medical treatment will be provided. Because the technical difficulties of traditional cell assays have limited the systematic study of cellular interactions, novel platforms with the ability to represent cellular interactions in a quick, spatiotemporal-resolved, and biomimetic manner would be welcomed by the research community. Microfluidics, one of the novel techniques used frequently for cell biology studies, is able to introduce the cellular interactions into an in vivo-like microenvironment with high spatiotemporal resolution, also allowing the quantification of cellular behaviors at the single cell level. The aim of this thesis is to develop biomimetic microfluidic platforms to mechanistically study cellular interactions in the context of different biological processes. First, Chapter 1 of this thesis reviews the application of microfluidics in the field of cellular interactions with focus on advances of microfluidics in single cell analysis and in vivo-like microenvironment generation. The following chapters separately discuss the topics including cell-drug interactions (Chapter 2), cell migration within complex gradient patterns (Chapter 3), the interactions between iii cell migration and angiogenesis growth (Chapter 4), heterotypic cellular interactions in a biomimetic environment (Chapter 5), and the effects of shear rates on cellular adhesion behaviors (Chapter 6). In Chapter 2, we developed a microfluidic platform containing stable chemical gradients to assess the drug effects on neutrophil migration, which is the key characteristic of inflammatory diseases. By tracking the migration of single neutrophils, we achieved quantification of various parameters, including average velocity, orientation, and overall effectiveness of migration. In addition to examining neutrophil migratory behaviors, the cytotoxicity of drug candidates was also evaluated to reveal a comprehensive understanding about the drug effects on neutrophil function. In Chapter3 and 4, we continued to study neutrophil migration in more complicated in vivo-like microenvironments. To be specific, a three-dimensional endothelial cell layer was cultured in the microfluidic channel, and neutrophil transendothelial migration was monitored under various chemical gradient patterns such that the competitive and synergistic effects among different cytokine molecules were determined. Furthermore, the interactions between neutrophil migration and…
Subjects/Keywords: biomimetic; cell migration; Cellular interaction; Microfluidics
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APA ·
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Manager
APA (6th Edition):
Wu, X. (2016). Development of Biomimetic Microfluidic Platforms for Cellular Interaction Studies. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/182827
Chicago Manual of Style (16th Edition):
Wu, Xiaojie. “Development of Biomimetic Microfluidic Platforms for Cellular Interaction Studies.” 2016. Doctoral Dissertation, University of Minnesota. Accessed January 21, 2021.
http://hdl.handle.net/11299/182827.
MLA Handbook (7th Edition):
Wu, Xiaojie. “Development of Biomimetic Microfluidic Platforms for Cellular Interaction Studies.” 2016. Web. 21 Jan 2021.
Vancouver:
Wu X. Development of Biomimetic Microfluidic Platforms for Cellular Interaction Studies. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/11299/182827.
Council of Science Editors:
Wu X. Development of Biomimetic Microfluidic Platforms for Cellular Interaction Studies. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/182827
University of Hong Kong
21.
杜潔欣.
Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment.
Degree: 2015, University of Hong Kong
URL: http://hdl.handle.net/10722/211113
Subjects/Keywords: Catenins; Cell
interaction
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
杜潔欣. (2015). Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/211113
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
杜潔欣. “Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment.” 2015. Thesis, University of Hong Kong. Accessed January 21, 2021.
http://hdl.handle.net/10722/211113.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
杜潔欣. “Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment.” 2015. Web. 21 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
杜潔欣. Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment. [Internet] [Thesis]. University of Hong Kong; 2015. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10722/211113.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
杜潔欣. Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment. [Thesis]. University of Hong Kong; 2015. Available from: http://hdl.handle.net/10722/211113
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
University of Hong Kong
22.
张栩.
Regulation of testicular
cell junction dynamics.
Degree: 2014, University of Hong Kong
URL: http://hdl.handle.net/10722/216333
► Spermatogenesis is an important cellular process in which mature haploid spermatozoa are produced from diploid spermatogonia and finally released from the seminiferous epithelium into the…
(more)
▼ Spermatogenesis is an important cellular
process in which mature haploid spermatozoa are produced from
diploid spermatogonia and finally released from the seminiferous
epithelium into the tubular lumen. This process involves extensive
and timely restructuring of cell junctions at the Sertoli-Sertoli
cell interface where the blood-testis barrier (BTB) and basal
ectoplasmic specialization (ES) are located and at the Sertoli-germ
cell interface where apical ES is present. The restructuring of
cell junctions is involved in facilitating the translocation of
spermatocytes across the BTB into adluminal compartment of the
seminiferous epithelium as well as the detachment of mature
elongated spermatids from the epithelium for release.
Turnover
of the BTB and Sertoli-mature spermatid contact (apical ES) can be
interrupted by environmental toxicant such as cadmium (Cd) and is
tightly regulated by cytokines such as transforming growth factor
betas (TGF-βs). However, the molecular mechanisms involved in the
regulation of testicular cell junction dynamics have not been
completely explored. This dissertation focused on examining the
mechanisms on how Cd and TGF-β3 regulate junction proteins,
nectin-2 and JAM-B respectively.
Nectin-2 is found at the basal
and apical ES for the formation of the BTB and Sertoli-spermatid
adhesion. Results of this study have demonstrated that CdCl2
reduces nectin-2 mRNA and protein levels and causes the loss of
nectin-2 at the cell-cell interface. Inhibitor and shRNA knockdown
have shown that CdCl2 speeds up nectin-2 protein degradation
through clathrin-mediated endocytosis. Immunofluorescence staining
and endocytosis assays further confirmed that nectin-2
internalization is promoted upon Cd treatment. In addition, Cd
causes nectin-2 gene repression by reducing the bioavailability of
endogenous transcription factors as well as impeding the binding of
positive regulators to the promoter region. Collectively, these
results have unraveled a novel and direct regulatory mechanism of
Cd-mediated male reproductive dysfunction and explain why testis is
more susceptible to Cd toxicity than other tissues.
Junctional
adhesion molecule-B (JAM-B) is found between Sertoli cells at the
BTB as well as between Sertoli and germ cells at the apical ES in
the testis. It was found that TGF-β3 downregulates JAM-B mRNA and
protein levels, and reduces the bioavailability of JAM-B at the
site of cell-cell contact. TGF-β3 promotes JAM-B protein
degradation via ubiquitin-proteasome pathway with the activation of
Smad signaling cascade. Besides, TGF-β3 destabilizes JAM-B mRNA
transcript via ERK1/2 and p54 JNK activation. These results have
unraveled the mechanisms by which TGF-β3 exerts negative regulatory
effects on JAM-B expression that is crucial for the BTB integrity
and Sertoli-germ cell interaction.
Taken together, the findings
reported herein demonstrate that testicular cell junction proteins
are regulated by environmental toxicant and cytokine at the
transcriptional, post-transcriptional and…
Subjects/Keywords: Cell
interaction;
Spermatogenesis
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APA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
张栩. (2014). Regulation of testicular
cell junction dynamics. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/216333
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
张栩. “Regulation of testicular
cell junction dynamics.” 2014. Thesis, University of Hong Kong. Accessed January 21, 2021.
http://hdl.handle.net/10722/216333.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
张栩. “Regulation of testicular
cell junction dynamics.” 2014. Web. 21 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
张栩. Regulation of testicular
cell junction dynamics. [Internet] [Thesis]. University of Hong Kong; 2014. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10722/216333.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
张栩. Regulation of testicular
cell junction dynamics. [Thesis]. University of Hong Kong; 2014. Available from: http://hdl.handle.net/10722/216333
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
University of Hong Kong
23.
杜潔欣.
Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment.
Degree: 2015, University of Hong Kong
URL: http://hdl.handle.net/10722/225149
► Dynamic interactions between cancer cells and the tumor microenvironment are increasingly recognized as contributors to cancer heterogeneity and increased therapeutic resistance. Tumor hypoxia and tumor-associated…
(more)
▼ Dynamic interactions between cancer cells and
the tumor microenvironment are increasingly recognized as
contributors to cancer heterogeneity and increased therapeutic
resistance. Tumor hypoxia and tumor-associated macrophages (TAMs)
are two characteristic elements common to many cancer types, and
aberrant β-catenin signaling is frequently observed in cancers. In
this study, the roles of β-catenin signaling in cancer cell
response to tumor hypoxia and TAMs were investigated. In several
human colorectal cancer(CRC)cell lines, hypoxic conditions
simultaneously increased the expression of β-catenin and the orphan
nuclear receptor Nur77. While β-catenin enhanced Nur77 mRNA
expression through hypoxia-inducible factor-1αinstead of T-cell
factor(TCF), Nur77 reciprocally increased β-catenin stability
independent of the adenomatous polyposis coli and p53 pathways, but
through Akt signaling. Overexpressed β-catenin and Nur77
synergistically stimulated migration, invasion, and
epithelial-mesenchymal transition of hypoxic CRCs. These findings
suggest a positive feedback circuit of β-catenin/Nur77, which could
enhance the invasive growth of hypoxic CRCs. To develop a new model
of spontaneous ovarian cancer metastasis, a paired cell lines with
contrasting metastatic potentials were selected. When
orthotopically implanted inimmunodeficient mice, the highly
metastatic (HM) line spontaneously formed peritoneal metastases and
ascites, thus resembling the major dissemination route of human
ovarian cancer. The non-metastatic (NM) counterpart also formed
primary tumor in the ovaries, but no metastasis was detected. Using
label-free quantitative liquid chromatography-tandem mass
spectrometry(LC-MS/MS)and pathway analysis, a prominent enrichment
of β-catenin signaling was found in HM. Western blotting and
reporter assays confirmed a significant increase in β-catenin
expression and TCF transcription activity in HM. Moreover,
β-catenin knockdown severely impaired tumor initiation and
metastasis of HM in mice. These results together highlight the
clinical relevance of β-catenin signaling in ovarian cancer.
Previous studies on TAMs usually inferred tumor cells as
homogeneous populations. However, recent data suggested the
existence of cell-to-cell variation. Using live-cell imaging to
track individual cells, NM and HM cells were found to react
differently upon M1 macrophage co-culture. Interestingly, M1
macrophages selectively promoted survival and induced a distinct
polyploid phenotype in HMs, which resulted from failed cytokinesis.
A contact-dependent interaction between metadherin on HMs and
CEACAM1 on macrophages could be important for inducing this
phenotype. β-catenin, which was specifically activated in HM, but
not NM, was responsible for the metadherin expression in HMs. On
the other hand, TAM markers and cytokines that are highly enriched
in ovarian cancer were also enhanced in macrophages by the
co-cultured HM. These findings suggest a positive crosstalk between
HM and macrophages that reinforces their respective phenotypic
changes.…
Subjects/Keywords: Catenins; Cell
interaction
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
杜潔欣. (2015). Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/225149
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
杜潔欣. “Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment.” 2015. Thesis, University of Hong Kong. Accessed January 21, 2021.
http://hdl.handle.net/10722/225149.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
杜潔欣. “Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment.” 2015. Web. 21 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
杜潔欣. Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment. [Internet] [Thesis]. University of Hong Kong; 2015. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/10722/225149.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
杜潔欣. Beta-catenin signaling in
the interactions of tumor cells and the tumor
microenvironment. [Thesis]. University of Hong Kong; 2015. Available from: http://hdl.handle.net/10722/225149
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation
24.
Tresier, Matthew David, 1981-.
Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:.
Degree: PhD, Biomedical Engineering, 2009, Rutgers University
URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914
► This dissertation advances the field of biomaterials-based tissue engineering via the development of a single cell imaging approach to profile and predict cellular responses. The…
(more)
▼ This dissertation advances the field of biomaterials-based tissue engineering via the development of a single cell imaging approach to profile and predict cellular responses. The methodology at the core of the dissertation characterizes cellular behaviors via the extraction and quantification of cell shape, intensity, textural and spatial distribution features of molecular fluororeporters, referred to as high-content cell imaging.
First, we highlight the use of high-content imaging of cell-based fluororeporters to establish and correlate quantifiable metrics of intracellular cytoskeletal features (e.g., descriptors of actin organization) on a set of model biomaterial substrates. The high-content imaging approach is then applied to spatially graded polymer blend substrates of both continuous roughness and discrete chemical compositions in parallel with high-throughput analyses. The imaging approach allowed us to identify the "global" and “high content” structure-property relationships between cell adhesion and biomaterial properties such as polymer chemistry and topography. The approach also identifies features of the actin-based cytoskeleton that respond to minute chemical modifications of the polymer backbone of a combinatorially derived library. In combination with decision tree and artificial neural network analyses, these 24-hour descriptors are used to predict 3-week material-mediated mineralization.
The high-content imaging approach is complemented with multi-dimensional scaling (MDS) modeling efforts to project amplified variations in cytoskeletal organization that forecast human mesenchymal stem cell lineage commitment before it is detected via traditional assays. Utilizing early quantitative measures of cytoskeletal morphology (morphometrics) and MDS allows the identification of distinct subpopulations of stem cells that emerge from non-linear combinations of cell shape, texture, density and cytoskeletal organizational features. These clusters allow the prediction of long-term differentiation behaviors of stem cells that manifest days to weeks later than the time of morphometric analysis. The proposed platform represents an ideal approach to probe cell-responses to complex microenvironments as it provides: real time measures of stem cell fates and material induced cell responses, cell-by-cell based analysis that captures the heterogeneity of sample populations, and the ability to parse out lineage commitment in stem cells resulting from multiple stimuli.
Advisors/Committee Members: Tresier, Matthew David, 1981- (author), Moghe, Prabhas (chair), Kohn, Joachim (co-chair), Knight, Doyle (internal member), Androulakis, Ioannis (internal member), Goss Kinzy, Terri (outside member).
Subjects/Keywords: Cell interaction
…CELLBIOMATERIAL INTERACTIONS
21
1.5 THE CYTOSKELETON AS A REGULATOR OF CELL BEHAVIOR
24
1.6 HYPOTHESIS… …STUDYING
CELL-MATERIAL INTERACTIONS
31
2.1 INTRODUCTION
2.2 MATERIALS AND METHODS
2.2.1 POLYMER… …SYNTHESIS AND FABRICATION
2.2.2 CELL CULTURE AND TRANSFECTION
2.2.3 IMAGING
2.2.4 MORPHOMETRIC… …RESPONSIVE CELL GFP REPORTERS
2.3.2 "HEAT MAP" REPRESENTATION OF CELL DESCRIPTORS FOR… …COMBINATORIAL
BIOMATERIALS
2.3.3 MORPHOMETRIC DESCRIPTORS OF CELL FUNCTIONAL FATES ON BIOMATERIALS
2.4…
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APA (6th Edition):
Tresier, Matthew David, 1. (2009). Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914
Chicago Manual of Style (16th Edition):
Tresier, Matthew David, 1981-. “Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:.” 2009. Doctoral Dissertation, Rutgers University. Accessed January 21, 2021.
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914.
MLA Handbook (7th Edition):
Tresier, Matthew David, 1981-. “Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:.” 2009. Web. 21 Jan 2021.
Vancouver:
Tresier, Matthew David 1. Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:. [Internet] [Doctoral dissertation]. Rutgers University; 2009. [cited 2021 Jan 21].
Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914.
Council of Science Editors:
Tresier, Matthew David 1. Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:. [Doctoral Dissertation]. Rutgers University; 2009. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914
Georgia State University
25.
He, Xiuxiu.
Integrated Mathematical and Experimental Study of Cell Migration and Shape.
Degree: PhD, Mathematics and Statistics, 2018, Georgia State University
URL: https://scholarworks.gsu.edu/math_diss/53
► Cell migration plays an essential role in many of physiological and pathological processes, including morphogenesis, inflammation, wound healing, and tumor metastasis. It is a…
(more)
▼ Cell migration plays an essential role in many of physiological and pathological processes, including morphogenesis, inflammation, wound healing, and tumor metastasis. It is a complex process that involves multi-scale interactions between the
cell and the extracellular matrix (ECM). Cells migrate through stromal ECM with native and
cell-derived curvature at micron-meter scale are context-specific. How does the curvature of ECM mechanically change
cell morphology and motility? Can the diverse migration behaviors from genetically identical cells be predictively using
cell migrating data? We address these questions using an integrated computational and experimental approach: we developed three-dimensional biomechanical
cell model and measured and analyzed a large number of
cell migration images over time. Our findings suggest that 1. substrate curvature determines
cell shape through contact and regulating protrusion dynamics; 2. effective
cell migration is characterized with long cellular persistence time, low speed variation, spatial-temporally coordinated protrusion and contraction; 3. the
cell shape variation space is low dimensional; and 4. migration behavior can be determined by a single image projected in the low dimensional
cell shape variation space.
Advisors/Committee Members: Yi Jiang.
Subjects/Keywords: Cell migration; Cancer invasion; Substrate curvature; Morphological dynamics; Cell-extracellular matrix interaction; Spatial-temporal interaction; Cell motility; Persistent random walk
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APA (6th Edition):
He, X. (2018). Integrated Mathematical and Experimental Study of Cell Migration and Shape. (Doctoral Dissertation). Georgia State University. Retrieved from https://scholarworks.gsu.edu/math_diss/53
Chicago Manual of Style (16th Edition):
He, Xiuxiu. “Integrated Mathematical and Experimental Study of Cell Migration and Shape.” 2018. Doctoral Dissertation, Georgia State University. Accessed January 21, 2021.
https://scholarworks.gsu.edu/math_diss/53.
MLA Handbook (7th Edition):
He, Xiuxiu. “Integrated Mathematical and Experimental Study of Cell Migration and Shape.” 2018. Web. 21 Jan 2021.
Vancouver:
He X. Integrated Mathematical and Experimental Study of Cell Migration and Shape. [Internet] [Doctoral dissertation]. Georgia State University; 2018. [cited 2021 Jan 21].
Available from: https://scholarworks.gsu.edu/math_diss/53.
Council of Science Editors:
He X. Integrated Mathematical and Experimental Study of Cell Migration and Shape. [Doctoral Dissertation]. Georgia State University; 2018. Available from: https://scholarworks.gsu.edu/math_diss/53
26.
Benomar, Saida.
Etude d'un écosystème bactérien synthétique anaérobie producteur d'hydrogène : Impact des interactions bactérie-bactérie sur le métabolisme : The aesthetics of ruins in war photography : Beirut as an examplary case.
Degree: Docteur es, Microbiologie, 2012, Aix Marseille Université
URL: http://www.theses.fr/2012AIXM4718
► Un grand nombre d'espèces microbiennes, issues d'environnements divers et présentant une large gamme de métabolismes différents, peuvent produire de l'hydrogène par voie fermentaire. Jusqu'à présent,…
(more)
▼ Un grand nombre d'espèces microbiennes, issues d'environnements divers et présentant une large gamme de métabolismes différents, peuvent produire de l'hydrogène par voie fermentaire. Jusqu'à présent, les études ont principalement porté sur l'utilisation de cultures microbiennes pures, voire génétiquement modifiées, en vue d'optimiser la production de biohydrogène à partir de sucres simples ou peu complexes. Les efforts de recherche portent désormais sur l'utilisation de cultures microbiennes mixtes pour produire du biohydrogène à partir de sources organiques plus complexes issus par exemple du traitement de la biomasse. Toutefois, la présence de voies métaboliques alternatives tout comme l'instabilité du processus biologique constitue des verrous scientifiques et techniques qu'il conviendra de lever pour une application potentielle. Ceci nécessite entre autre une meilleur connaissance des interactions bactériennes et donc métaboliques présentent au sein du consortium.Pour cela, nous avons développé une approche innovante et pluridisciplinaire d'ingénierie écologique qui consiste en la conception, la construction et l'étude d'un consortium microbien synthétique afin d'établir les paramètres régissant les réseaux d'interactions métaboliques avec pour objectif d'optimiser la production d'hydrogène. Dans un premier temps nous avons choisit d'étudier les réseaux d'interactions métaboliques entre deux souches modèles connues comme partie prenante d'un consortium bactérien naturel, une bactérie du genre Clostridie et une du genre Desulfovibrio, la première étant productrice d'hydrogène par fermentation
Numerous microorganisms can produce hydrogen by “dark fermentation”. Isolated from various environments, they present a broad range of different metabolisms. Until now, literature reports have mainly dealt with the use of pure microbial cultures producing biohydrogen from simple sugars, such as glucose and sucrose. More recently, studies on biohydrogen production by mixed cultures from complex organic sources have been developed. Even though biohydrogen productivities and conversion yields can be interesting for industrial purposes, several scientific and technical constraints remain to be addressed. In particular, the presence of alternative metabolic ways of hydrogen consumption generally results in chronic instability of the biological processes. To increase the stability and the efficiency of dark fermentative processes, it is now necessary to acquire a better understanding of the metabolic interaction networks existing between producing and consuming microorganisms.We have developed an innovative and multidisciplinary approach to ecological engineering, which consists of the construction and study of synthetic microbial consortia to establish the metabolic networks existing between microorganisms for further optimization of biohydrogen production. First we have studied the networks of metabolic interactions between two bacterial models known as involved in a natural bacterial consortium: a bacterium from Clostridium…
Advisors/Committee Members: Giudici-Orticoni, Marie-Thérèse (thesis director).
Subjects/Keywords: Communauté microbienne; Bioénergie et hydrogène; Microbiologie; Interaction cell-cell; Anaérobie; Biofilm; Microbial community; Bioenergy and hydrogen; Microbiology; Cell-cell interaction; Anaerobic; Biofilm
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APA ·
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APA (6th Edition):
Benomar, S. (2012). Etude d'un écosystème bactérien synthétique anaérobie producteur d'hydrogène : Impact des interactions bactérie-bactérie sur le métabolisme : The aesthetics of ruins in war photography : Beirut as an examplary case. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2012AIXM4718
Chicago Manual of Style (16th Edition):
Benomar, Saida. “Etude d'un écosystème bactérien synthétique anaérobie producteur d'hydrogène : Impact des interactions bactérie-bactérie sur le métabolisme : The aesthetics of ruins in war photography : Beirut as an examplary case.” 2012. Doctoral Dissertation, Aix Marseille Université. Accessed January 21, 2021.
http://www.theses.fr/2012AIXM4718.
MLA Handbook (7th Edition):
Benomar, Saida. “Etude d'un écosystème bactérien synthétique anaérobie producteur d'hydrogène : Impact des interactions bactérie-bactérie sur le métabolisme : The aesthetics of ruins in war photography : Beirut as an examplary case.” 2012. Web. 21 Jan 2021.
Vancouver:
Benomar S. Etude d'un écosystème bactérien synthétique anaérobie producteur d'hydrogène : Impact des interactions bactérie-bactérie sur le métabolisme : The aesthetics of ruins in war photography : Beirut as an examplary case. [Internet] [Doctoral dissertation]. Aix Marseille Université 2012. [cited 2021 Jan 21].
Available from: http://www.theses.fr/2012AIXM4718.
Council of Science Editors:
Benomar S. Etude d'un écosystème bactérien synthétique anaérobie producteur d'hydrogène : Impact des interactions bactérie-bactérie sur le métabolisme : The aesthetics of ruins in war photography : Beirut as an examplary case. [Doctoral Dissertation]. Aix Marseille Université 2012. Available from: http://www.theses.fr/2012AIXM4718
27.
Higa, Guilherme Shigueto Vilar.
Possível relação entre acoplamento e ciclo celular na neurodegeneração da retina.
Degree: Mestrado, Fisiologia Humana, 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/42/42137/tde-29012013-085055/
;
► A sinalização no sistema nervoso pode ocorrer pelo acoplamento direto entre células, via canais de junção comunicantes (JCs). Estes canais permitem a passagem de moléculas…
(more)
▼ A sinalização no sistema nervoso pode ocorrer pelo acoplamento direto entre células, via canais de junção comunicantes (JCs). Estes canais permitem a passagem de moléculas de até 1 kDa, e são formados por subunidades proteicas denominadas conexinas (Cxs). A comunicação celular via JCs desempenha um importante papel durante o desenvolvimento e a sinalização visual. Além disso, o acoplamento celular provido pelas JCs tem sido relacionado a processos de sobrevivência/morte celular. Do mesmo modo, ciclinas e cinases dependentes de ciclinas (CDKs), além de seu papel clássico na regulação do ciclo e diferenciação celular, estão envolvidas em processos neurodegenerativos. Estudos recentes têm observado a reentrada no ciclo celular de células neuronais pós-mitóticas em apoptose. Neste contexto, analisamos a expressão gênica e proteica das Cxs e ciclinas em resposta às lesões no sistema visual, especificamente na retina. Estas análises foram realizadas após a indução de trauma mecânico, modelo experimental que permite a visualização do foco, penumbra e áreas adjacentes à lesão. Utilizando técnicas combinadas, como a reação em cadeia da polimerase em tempo real (PCR Real-Time), Western Blot e imuno-histoquímica, avaliamos a expressão espaço-temporal destes genes e as proteínas por eles codificadas, em diferentes tempos pós-lesão. Os resultados da PCR Real-Time revelaram uma ausência de modulação da expressão gênica de Cx36 para os diferentes tempos pós-lesão analisados. A Cx43 mostrou aumento dos transcritos, após três e sete dias pós-lesão. A ciclina D1 e B1 apresentaram modulação significativa após um, três e sete dias pós-lesão. As análises de imuno-histoquímica revelaram uma redistribuição da Cx36 em resposta à lesão em diferentes tempos pós-lesão. A Cx43, por sua vez, apresentou um aumento aparente de sua expressão no foco e zona de penumbra da lesão, nos período de um, três e sete dias pós-lesão. Em retinas, após um e três dias de lesão, a ciclina D1 encontrou-se presente em células próximas ao foco da lesão. Observamos a presença de ciclina B1 no foco da lesão após um dia de lesão. Por meio de análises de Western Blot não foi possível detectar alterações das diferentes proteínas estudadas nas retinas, em períodos variados de exposição à lesão. Os dados deste estudo sugerem que i) possivelmente, as células afetadas pela lesão se encontram acopladas; ii) expressam proteínas reguladoras do ciclo celular. Levando em consideração o conjunto de resultados, sugerimos que é possível induzir ou prevenir a reentrada do ciclo celular em células pós-mitóticas da retina, controlando o acoplamento mediado pelas proteínas formadoras das JC.
Communication in the nervous system can occur directly between the cells through structures known as gap junction (GJ) channels. These channels allow the passage of small molecules up to 1 kDa and are composed of protein subunits named connexins (Cxs). Cell communication through GJ plays an important role during the development and visual signaling. Furthermore, cell coupling provided by the GJs…
Advisors/Committee Members: Kihara, Alexandre Hiroaki.
Subjects/Keywords: Cell cycle; Cell interaction; Ciclo celular; Conexinas; Connexins; Interação celular; Retina; Retina
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APA ·
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MLA ·
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APA (6th Edition):
Higa, G. S. V. (2012). Possível relação entre acoplamento e ciclo celular na neurodegeneração da retina. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42137/tde-29012013-085055/ ;
Chicago Manual of Style (16th Edition):
Higa, Guilherme Shigueto Vilar. “Possível relação entre acoplamento e ciclo celular na neurodegeneração da retina.” 2012. Masters Thesis, University of São Paulo. Accessed January 21, 2021.
http://www.teses.usp.br/teses/disponiveis/42/42137/tde-29012013-085055/ ;.
MLA Handbook (7th Edition):
Higa, Guilherme Shigueto Vilar. “Possível relação entre acoplamento e ciclo celular na neurodegeneração da retina.” 2012. Web. 21 Jan 2021.
Vancouver:
Higa GSV. Possível relação entre acoplamento e ciclo celular na neurodegeneração da retina. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 21].
Available from: http://www.teses.usp.br/teses/disponiveis/42/42137/tde-29012013-085055/ ;.
Council of Science Editors:
Higa GSV. Possível relação entre acoplamento e ciclo celular na neurodegeneração da retina. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/42/42137/tde-29012013-085055/ ;
University of Alberta
28.
Masellis-Smith, Anna.
Interaction with CD9 regulates cellular adhesion within the
B cell lineage.
Degree: PhD, Department of Medicine, 1993, University of Alberta
URL: https://era.library.ualberta.ca/files/p2676x418
Subjects/Keywords: Cell interaction.; B cells.; Cell adhesion.
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APA (6th Edition):
Masellis-Smith, A. (1993). Interaction with CD9 regulates cellular adhesion within the
B cell lineage. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/p2676x418
Chicago Manual of Style (16th Edition):
Masellis-Smith, Anna. “Interaction with CD9 regulates cellular adhesion within the
B cell lineage.” 1993. Doctoral Dissertation, University of Alberta. Accessed January 21, 2021.
https://era.library.ualberta.ca/files/p2676x418.
MLA Handbook (7th Edition):
Masellis-Smith, Anna. “Interaction with CD9 regulates cellular adhesion within the
B cell lineage.” 1993. Web. 21 Jan 2021.
Vancouver:
Masellis-Smith A. Interaction with CD9 regulates cellular adhesion within the
B cell lineage. [Internet] [Doctoral dissertation]. University of Alberta; 1993. [cited 2021 Jan 21].
Available from: https://era.library.ualberta.ca/files/p2676x418.
Council of Science Editors:
Masellis-Smith A. Interaction with CD9 regulates cellular adhesion within the
B cell lineage. [Doctoral Dissertation]. University of Alberta; 1993. Available from: https://era.library.ualberta.ca/files/p2676x418
University of Michigan
29.
Kim, Hong Sun.
Targeting Head and Neck Cancer Stem Cells and Endothelial Cell Interaction.
Degree: PhD, Cancer Biology, 2016, University of Michigan
URL: http://hdl.handle.net/2027.42/133361
► Tumor recurrence and loco-regional metastases are the major clinical challenges in the management of patients with head and neck squamous cell carcinoma (HNSCC). A subpopulation…
(more)
▼ Tumor recurrence and loco-regional metastases are the major clinical challenges in the management of patients with head and neck squamous
cell carcinoma (HNSCC). A subpopulation of cells, called cancer stem cells, has been identified in HNSCC and characterized as cells with uniquely high tumor-forming ability and resistance to conventional chemotherapies. Multiple reports have shown that head and neck cancer stem cells drive the metastatic process. How tumorigenic cells are displaced from the tumor “island”, travel through connective tissues, and get into blood vessels is still not well understood. We hypothesized that endothelial
cell-secreted factors generate a chemotactic gradient that attracts head and neck cancer stem cells towards blood vessels. Head and neck cancer stem cells reside in perivascular niches. Close proximity between cancer stem cells and blood vessels may facilitate cancer stem cells to invade into the bloodstream and initiate metastasis. Our group previously has shown that endothelial
cell-secreted factors, specifically interleukin-6 (IL-6), potentiate the self-renewal and tumorigenicity of head and neck cancer stem cells. Here, we assessed the role of endothelial
cell-initiated IL-6 pathway activation in head and neck cancer stem
cell motility. Endothelial
cell-secreted IL-6 induced the expression of the mesenchymal marker Vimentin and epithelial-mesenchymal transition-activating transcription factor Snail in head and neck cancer stem cells. When endothelial
cell-secreted IL-6 was inhibited, we observed decreased motility in head and neck cancer stem cells. Xenograft HNSCC tumors vascularized with IL-6 knockout human endothelial cells grew slower than tumors vascularized with control endothelial cells. Notably, tumors grown with IL-6 knockout endothelial cells had smaller fraction of cancer stem cells than those with control endothelial cells. In addition, anti-IL-6 receptor (IL-6R) antibody, tocilizumab, also decreased cancer stem
cell population. Tissue microarray analysis of 80 HNSCC patient samples revealed that high IL-6R or its co-receptor gp130 expressions correlated with low patient survival. Taken together, these results highlight the contribution of endothelial
cell secreted factors on the migratory behavior of head and neck cancer stem
cell that ultimately result in dissemination of tumor cells. Further, we show the therapeutic potential of tocilizumab targeting cancer stem cells in head and neck cancer.
Advisors/Committee Members: Nor, Jacques Eduardo (committee member), Kapila, Yvonne (committee member), Duckett, Colin S (committee member), Zou, Weiping (committee member).
Subjects/Keywords: head and neck cancer stem cell and endothelial cell interaction; Science (General); Science
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APA (6th Edition):
Kim, H. S. (2016). Targeting Head and Neck Cancer Stem Cells and Endothelial Cell Interaction. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/133361
Chicago Manual of Style (16th Edition):
Kim, Hong Sun. “Targeting Head and Neck Cancer Stem Cells and Endothelial Cell Interaction.” 2016. Doctoral Dissertation, University of Michigan. Accessed January 21, 2021.
http://hdl.handle.net/2027.42/133361.
MLA Handbook (7th Edition):
Kim, Hong Sun. “Targeting Head and Neck Cancer Stem Cells and Endothelial Cell Interaction.” 2016. Web. 21 Jan 2021.
Vancouver:
Kim HS. Targeting Head and Neck Cancer Stem Cells and Endothelial Cell Interaction. [Internet] [Doctoral dissertation]. University of Michigan; 2016. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/2027.42/133361.
Council of Science Editors:
Kim HS. Targeting Head and Neck Cancer Stem Cells and Endothelial Cell Interaction. [Doctoral Dissertation]. University of Michigan; 2016. Available from: http://hdl.handle.net/2027.42/133361
University of Toronto
30.
Xu, Songyi.
N-cadherin Regulation of Vascular Smooth Muscle Cells; the Role of DDR1 and Rho GTPases.
Degree: PhD, 2020, University of Toronto
URL: http://hdl.handle.net/1807/101328
► N-cadherin mediates cell-cell contacts in vascular smooth muscle cells (VSMCs) and regulates VSMC behaviours. Discoidin domain receptor 1 (DDR1) is a collagen binding receptor also…
(more)
▼ N-cadherin mediates
cell-
cell contacts in vascular smooth muscle cells (VSMCs) and regulates VSMC behaviours. Discoidin domain receptor 1 (DDR1) is a collagen binding receptor also implicated in these processes. Previous studies showed that both N-cadherin and DDR1 are upregulated after vascular injury, but it is not known whether the two molecules are associated. The ability to establish proper N-cadherin junctions is important in regulating VSMC behaviour. In my studies, I found that N-cadherin could associate with DDR1, and that this association was increased during VSMC migration and wound closure. I found that N-cadherin was reduced in lipid rafts and mislocalized from
cell-
cell junctions in the absence of DDR1. Disruption of lipid rafts by cholesterol oxidase or methyl-β-cyclodextrin removed N-cadherin from lipid rafts of DDR1+/+ VSMCs and disrupted N-cadherin contacts, and these effects were reversed by cholesterol rescue that restored lipid rafts. Knockdown of DDR1 by siRNA resulted in mislocalized N-cadherin contacts from
cell junctions, and transfection of DDR1-/- VSMCs with full-length DDR1 rescued the formation of N-cadherin junctions. The mislocalization of N-cadherin contacts in DDR1-/- VSMCs was also accompanied by disruption of the actin cytoskeleton. N-cadherin contacts in VSMCs undergo constant remodeling, especially during disease processes. Calcium switch to disrupt and restore contacts did not affect N-cadherin triton solubility in DDR1+/+ and DDR1-/- VSMCs or the association between N-cadherin and DDR1. While collagen stimulation of DDR1 increased the association between N-cadherin and DDR1 in cells with mature, well-established
cell contacts, it did not affect N-cadherin localization, its triton solubility, or the association between N-cadherin and DDR1 during contact disruption, restoration, and wound closure. Rho GTPases are master regulators of the cytoskeleton and can mediate cytoskeletal processes downstream of
cell-
cell contacts. Using calcium switch and plating on either IgG or N-cadherin-Fc coated surfaces, active Rho GTPase pull-down assays showed a consistent decrease in Cdc42 and RhoA activity after N-cadherin contact establishment in DDR1+/+ SMCs. Overall, these data reveal that N-cadherin
cell-
cell contacts in VSMCs can be regulated through
interaction with DDR1 and localization in lipid rafts, and that the establishment of N-cadherin contacts can suppress Rho GTPase activity.
Advisors/Committee Members: Bendeck, Michelle, Laboratory Medicine and Pathobiology.
Subjects/Keywords: Cell-cell interaction; DDR1; N-cadherin; Rho GTPases; Vascular smooth muscle cells; 0307
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APA (6th Edition):
Xu, S. (2020). N-cadherin Regulation of Vascular Smooth Muscle Cells; the Role of DDR1 and Rho GTPases. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/101328
Chicago Manual of Style (16th Edition):
Xu, Songyi. “N-cadherin Regulation of Vascular Smooth Muscle Cells; the Role of DDR1 and Rho GTPases.” 2020. Doctoral Dissertation, University of Toronto. Accessed January 21, 2021.
http://hdl.handle.net/1807/101328.
MLA Handbook (7th Edition):
Xu, Songyi. “N-cadherin Regulation of Vascular Smooth Muscle Cells; the Role of DDR1 and Rho GTPases.” 2020. Web. 21 Jan 2021.
Vancouver:
Xu S. N-cadherin Regulation of Vascular Smooth Muscle Cells; the Role of DDR1 and Rho GTPases. [Internet] [Doctoral dissertation]. University of Toronto; 2020. [cited 2021 Jan 21].
Available from: http://hdl.handle.net/1807/101328.
Council of Science Editors:
Xu S. N-cadherin Regulation of Vascular Smooth Muscle Cells; the Role of DDR1 and Rho GTPases. [Doctoral Dissertation]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/101328
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Open Access Theses and Dissertations
