subject:(Caspase 3)

University of Edinburgh

1. Mackay, Martha. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.

Degree: PhD, 2014, University of Edinburgh

► The design and development of fluorescent reporters focussed on highly sensitive, specific, and selective imaging of cancer targets is described. These novel optical molecular probes… (more)

Subjects/Keywords: 616.99; Caspase-3; molecular detection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mackay, M. (2014). The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/17972

Chicago Manual of Style (16^th Edition):

Mackay, Martha. “The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed April 22, 2021. http://hdl.handle.net/1842/17972.

MLA Handbook (7^th Edition):

Mackay, Martha. “The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.” 2014. Web. 22 Apr 2021.

Vancouver:

Mackay M. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/1842/17972.

Council of Science Editors:

Mackay M. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/17972

Univerzitet u Beogradu

2. Dožić, Branko S., 1968-. Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda.

Degree: Medicinski fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get

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Medicina / Medicine

Adenoidni cistični karcinom (ACC), je jedan od najčešćih malignih tumora pljuvačnih žlezda. Učestvuje sa oko 10-15% kod salivarnih neoplazmi. Karakteriše ga spor… (more)

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Medicina / Medicine

Adenoidni cistični karcinom (ACC), je jedan od najčešćih malignih tumora pljuvačnih žlezda. Učestvuje sa oko 10-15% kod salivarnih neoplazmi. Karakteriše ga spor rast, visoka incidenca ka invaziji nerva, retko regionalno metastaziranje, česti lokalni recidivi i spor razvoj progresivnih i relativno indolentnih udaljenih metastaza. Najznačajniji faktori za prognozu ovog tumora su klinički stadijum i histološki izgled tumora, prisustvo ili odsustvo tumorskog tkiva na linijama resekcije, invazija nerva. Apoptoza je regulatorni mehanizam tkivne homeostaze. Poremećaj regulacije apoptoze je relativno česta karakteristika maligne ćelije. U zavisnosti od stimulansa, putevi aktivacije apoptoze mogu biti: receptorski (spoljašnji) i mitohondrijski (unutrašnji) put. U unutrašnjem putu apoptoze značajnu ulogu igraju Apaf-1, inicijatorne i efektorne kaspaze. Apaf-1 je multidomenski protein, za koji se vezuje citohrom c i prokaspaza 9 što dovodi do formiranja apoptozoma. Ovaj kompleks proteolitički aktivira inicijatornu kaspazu-9. U završnoj fazi apoptoze, aktivirana kaspaza 9 dovodi do aktivacije efektorne kaspaze 3, čijim ulaskom u jedro započinje degradaciona faza apoptoze. Cilj U ovoj studiji cilj je bio ispitati imunohistohemijsku ekspresiju Apaf-1, kaspaze 9 i 3 u ACC pljuvačnih žlezda, i rezultate korelirati sa kliničko-patološkim parametrima kako bi se utvrdilo koji je od njih nezavistan prognostički parametar. Materijal i metode Istraživanje je koncipirano kao studija preseka, koja obuhvata 50 pacijenata sa ACC pljuvačnih žlezda, muškog i ženskog pola, prosečne starosti 58 godina. U ovoj studiji koristili smo tehniku tkivnog mikroniza (TMA kalupi). Preseci sa TMA kalupa, debljine 5μm, bojeni su streptavidin-biotin imunohistohemijskom tehnikom pomoću primarnih antitela specifičnih za: Apaf-1, kaspazu 9 i kaspazu 3. Obojeni tkivni preseci su analizirani svetlosnim mikroskopom (Olympus tip BH-2). Na osnovu prikupljenih podataka formirana je baza podataka u –SPSS 18.0 koja je korišćena za dalju statističku obradu. Statistička analiza podataka obuhvatala je metode deskriptivne i analitičke (inferencijalne) statistike...

Advisors/Committee Members: Boričić, Ivan, 1956-.

Subjects/Keywords: adenoid cystic carcinoma; apoptosis; Apaf-1; caspase 9; caspase 3.

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APA (6^th Edition):

Dožić, Branko S., 1. (2016). Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dožić, Branko S., 1968-. “Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda.” 2016. Thesis, Univerzitet u Beogradu. Accessed April 22, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dožić, Branko S., 1968-. “Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda.” 2016. Web. 22 Apr 2021.

Vancouver:

Dožić, Branko S. 1. Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Apr 22]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dožić, Branko S. 1. Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago

3. Douglas, Hollian Raphaelle. XIAP, a potential regulator of follicular atresia in the sheep ovary .

Degree: 2011, University of Otago

URL: http://hdl.handle.net/10523/1635

► X-linked inhibitor of apoptosis (XIAP) is a strong inhibitor of initiator (-9) and executer (-3 and -7) caspase activity (Eckelman and Salvesen, 2006). In female… (more)

▼ X-linked inhibitor of apoptosis (XIAP) is a strong inhibitor of initiator (-9) and executer (-3 and -7) caspase activity (Eckelman and Salvesen, 2006). In female reproductive research, XIAP has received minimal attention considering the ability to arrest caspases provides a potential mechanism for regulation of follicular atresia. This study aimed to test the hypotheses that XIAP is indicative of follicular health and regulates follicular atresia and to investigate the novel idea that prolactin (PRL) stimulates XIAP expression in the sheep ovary. Estrous cycles of adult Romney ewes (N=31) were synchronized with Estrumate®. Tissue and blood samples were subsequently collected on either days 14, 15 and 16 or at twelve hour intervals during the follicular phase. Initially analysis involved developing a plasma PRL profile by radioimmunoassay. The presence and localization patterns of XIAP and prolactin receptor (PRLR) isoforms protein and/or mRNA were determined by RT-PCR, in situ hybridization histochemistry and immunohistochemistry. This was followed by a comparative study evaluating levels of active caspase-3 immunoreactivity and XIAP mRNA (N=22) or protein (N=23) expression in adjacent sections containing the same day 14, 15 and 16 antral follicles. Triple label immunofluorescence and confocal microscopy were subsequently used to further clarify XIAP and active caspase-3 protein localization and to establish the presence of an inverse expression relationship in granulosa and thecal cells. Differing doses of Ovine FSH and PRL, as potential stimuli of XIAP upregulation, were trialed in cultured granulosa cells. Finally, a provisional attempt to develop a granulosa cell death model for determination of PRL’s ability to upregulate XIAP was undertaken. This study showed widespread XIAP expression during both luteal and follicular phases of the estrous cycle. XIAP protein was detected from the primary follicle stage onwards, whereas the mRNA was only evident in antral follicles, likely due to limited sensitivity of the in situ hybridization histochemical technique. This also proved problematic for detection of PRLR isoform mRNA, which was localized in luteal cells and three antral follicles only. In the comparative analysis, all day 14 antral follicles showed positive XIAP mRNA expression irrespective of active caspase-3 levels. Over 50% of the day 15 and 16 antral follicles scored, however, showed an inverse relationship between XIAP mRNA/protein and active caspase-3 protein levels in, as did XIAP protein in day 14 antral follicles. Interestingly, in healthy follicles XIAP expression in thecal tissue was widespread, becoming increasingly localized to interna layers as active caspase-3 immunopositive granulosa cells became prevalent, despite a lack of caspase activity in the theca itself. Confocal microscopy showed active caspase- 3 and XIAP colocalization in granulosa cells. No significant negative correlation in expression patterns was observed, possibly due to brief active caspase-3 expression at apoptosis onset… Advisors/Committee Members: Hurst, Peter (advisor).

Subjects/Keywords: Ovary; XIAP; Active caspase-3; Follicle

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APA (6^th Edition):

Douglas, H. R. (2011). XIAP, a potential regulator of follicular atresia in the sheep ovary . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1635

Chicago Manual of Style (16^th Edition):

Douglas, Hollian Raphaelle. “XIAP, a potential regulator of follicular atresia in the sheep ovary .” 2011. Doctoral Dissertation, University of Otago. Accessed April 22, 2021. http://hdl.handle.net/10523/1635.

MLA Handbook (7^th Edition):

Douglas, Hollian Raphaelle. “XIAP, a potential regulator of follicular atresia in the sheep ovary .” 2011. Web. 22 Apr 2021.

Vancouver:

Douglas HR. XIAP, a potential regulator of follicular atresia in the sheep ovary . [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/10523/1635.

Council of Science Editors:

Douglas HR. XIAP, a potential regulator of follicular atresia in the sheep ovary . [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1635

North Carolina State University

4. Walters, Jad Anthony. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.

Degree: PhD, Biochemistry, 2009, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/3363

► Effector procaspase-3 plays a vital role in carrying out the final steps of programmed cell death, leading to the destruction of the cell. Because dimerization… (more)

Subjects/Keywords: Activation; Inhibition; Dimer Interface; (Pro)caspase-3

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APA (6^th Edition):

Walters, J. A. (2009). Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3363

Chicago Manual of Style (16^th Edition):

Walters, Jad Anthony. “Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.” 2009. Doctoral Dissertation, North Carolina State University. Accessed April 22, 2021. http://www.lib.ncsu.edu/resolver/1840.16/3363.

MLA Handbook (7^th Edition):

Walters, Jad Anthony. “Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.” 2009. Web. 22 Apr 2021.

Vancouver:

Walters JA. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. [Internet] [Doctoral dissertation]. North Carolina State University; 2009. [cited 2021 Apr 22]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3363.

Council of Science Editors:

Walters JA. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. [Doctoral Dissertation]. North Carolina State University; 2009. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3363

5. Mariana Caroline Furian Pontin. Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse.

Degree: 2018, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/

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O estresse causa modificações no epitélio intestinal, tais como o aumento de células caliciformes e da taxa de apoptose. O uso de alimentos nutracêuticos tem… (more)

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O estresse causa modificações no epitélio intestinal, tais como o aumento de células caliciformes e da taxa de apoptose. O uso de alimentos nutracêuticos tem sido uma alternativa para amenizar essas modificações sobre o tecido epitelial. Desta forma, este trabalho teve como objetivo avaliar se a inclusão de colostro bovino, o qual é constituído de fatores antioxidantes, imunes e de crescimento, seria capaz de amenizar as consequências do estresse crônico sob o intestino. Para isso, juvenis de pacu (Piaractus mesopotamicus) adensados a 50 kg/m3 foram alimentados duas vezes ao dia até a saciedade com ração peletizada e semi-purificada sem (0%CBL) e com a inclusão de colostro bovino liofilizado em concentrações crescentes (10, 20 e 30%CBL), (n=4). Após 28 dias, foram coletados segmentos do intestino médio, S1 e S2, e reto. Os tecidos foram marcados com corantes histológicos para a quantificação de células caliciformes contendo mucinas neutras, ácidas (incluindo sialo e sulfomucinas) e ácidas-neutras. Também foram mensurados o volume (Vv) e a densidade da superfície (Sv) da mucosa, por análise estereológica, e a espessura da camada muscular. A razão do número de cada tipo e subtipo de célula caliciforme sobre o Vv e Sv foi calculada para estimar a densidade de células caliciformes, Dv e Ds, respectivamente. A taxa apoptótica foi analisada qualitativamente através da intensidade (alta, média e baixa) da imunomarcação da caspase-3 nas células epiteliais. As dietas não influenciaram os parâmetros zootécnicos analisados (P>0,05). No reto, os grupos que receberam 20 e 30%CBL apresentaram menor número de células caliciformes contendo sulfomucinas e menor Ds em relação a 0 e 10% (P=0,0148 e 0,0198, respectivamente). No RT, Dv total e Dv de células caliciformes contendo mucinas ácidas foi maior em 0 e 30%CBL em relação a 20%CBL (P=0,0155 e 0,225, respectivamente). No S1, 10 e 30%CBL apresentaram maior Dv em relação a 20%CBL (P=0,0540). A espessura da camada muscular, o Vv e a Sv não diferiram entre os tratamentos (P>0,05). No S2 e RT, a taxa de apoptose teve relação inversa à concentração de colostro bovino liofilizado adicionado na ração. Nos três segmentos, houve maior proporção de células caliciformes contendo mucinas ácidas do que neutras, sendo a maioria representada por sulfomucinas. Assim, a inclusão de colostro bovino liofilizado nas rações de juvenis de pacu adensados diminuiu a apoptose nos segmentos intestinais S2 e RT e também diminuiu o número de células caliciformes contendo sulfomucinas no RT, indicando que o colostro bovino liofilizado pode ser utilizado como alimento nutracêutico para pacus (Piaractus mesopotamicus) adensados, a fim de diminuir a taxa apoptótica e proteger o intestino contra enzimas bacterianas, uma das principais funções das sulfomucinas.

The stress causes changes in the intestinal epithelium, such as the increase in the number of goblet cells and on the rate of apoptosis. The use of nutraceutical foods has been an alternative to soften these modifications on the epithelial tissue.…

Advisors/Committee Members: Débora Botequio Moretti, Jose Roberto Ferreira, Raul Machado Neto, Rafael Estevan Sabioni.

Subjects/Keywords: Adensamento; Caspase-3; Célula caliciforme; Teleósteo; Caspase-3; Goblet cell; Stocking density; Teleoste

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APA (6^th Edition):

Pontin, M. C. F. (2018). Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/

Chicago Manual of Style (16^th Edition):

Pontin, Mariana Caroline Furian. “Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse.” 2018. Masters Thesis, University of São Paulo. Accessed April 22, 2021. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/.

MLA Handbook (7^th Edition):

Pontin, Mariana Caroline Furian. “Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse.” 2018. Web. 22 Apr 2021.

Vancouver:

Pontin MCF. Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse. [Internet] [Masters thesis]. University of São Paulo; 2018. [cited 2021 Apr 22]. Available from: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/.

Council of Science Editors:

Pontin MCF. Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse. [Masters Thesis]. University of São Paulo; 2018. Available from: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/

6. Amptoulach, Sousana. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.

Degree: 2015, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/41730

► There is strong evidence that tumor growth is not only a result of uncontrolled cell proliferation but alsoof decreased apoptosis. Caspase-3 is a member of… (more)

Subjects/Keywords: Κασπάση-3; Απόπτωση; Γαστρικός καρκίνος; Caspase-3; Apoptosis; Gasric cancer

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APA (6^th Edition):

Amptoulach, S. (2015). Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/41730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amptoulach, Sousana. “Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.” 2015. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 22, 2021. http://hdl.handle.net/10442/hedi/41730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amptoulach, Sousana. “Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.” 2015. Web. 22 Apr 2021.

Vancouver:

Amptoulach S. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/10442/hedi/41730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amptoulach S. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. Available from: http://hdl.handle.net/10442/hedi/41730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

7. Carvalho, Virginia Campos, 1987-. Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ .

Degree: Faculdade de Ciências Médicas; Programa de Pós-Graduação em Clínica Médica, 2019, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504

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Orientador: Ricardo de Lima Zollner

Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas

Made available in DSpace on 2019-11-22T12:49:27Z (GMT). No. of… (more)

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Orientador: Ricardo de Lima Zollner

Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas

Made available in DSpace on 2019-11-22T12:49:27Z (GMT). No. of bitstreams: 1 Carvalho_VirginiaDeCampos_M.pdf: 1050903 bytes, checksum: aaeb468aa719a6f984e07946417f4dc1 (MD5) Previous issue date: 2019

Resumo: Plantas medicinais apresentam historicamente um papel importante no desenvolvimento de medicamentos. Doenças inflamatórias e autoimunes são cada vez mais frequentes na população, como o diabetes mellitus tipo 1A (DM-1A), o que impulsiona a investigação do potencial terapêutico de compostos naturais. Modelos experimentais são utilizados para testar novos compostos com potencial efeito farmacológico e permitir melhor compreensão de seus mecanismos de ação. Os camundongos da linhagem NOD/ShiLtJ são utilizados, para estudos relacionados ao DM-1A por desenvolverem a doença espontaneamente, de maneira similar ao que ocorre nos humanos. A Farmacopeia Brasileira descreve Passiflora sp (maracujá) como fitoterápico, estando relacionado ao tratamento de diversas doenças, o maracujá possui também ação hipoglicemiante, propriedade importante no controle do diabetes. Nosso grupo investigou a atividade antioxidante do extrato aquoso das folhas do maracujá doce (Passiflora alata Curtis) e sua ação em camundongos não obesos diabéticos (NOD), modelo experimental do DM-1A, O tratamento com extrato aquoso das folhas de Passiflora alata Curtis (EAFPA), diminuiu a incidência do diabetes, possivelmente por sua ação antiproliferativa e antioxidante. Tal efeito parece estar associado com a diminuição do infiltrado de células inflamatórias, como linfócitos T CD4+ e CD8+ nas ilhotas pancreáticas (insulite). Neste contexto, investigamos o efeito in vitro do extrato aquoso liofilizado da folha de EAFPA (100, 200 e 500 µg/mL) na indução de morte celular por apoptose em linfócitos T CD4+ e CD8+ provenientes de camundongos NOD/ShiLtJ (não diabéticos). Para esta finalidade, avaliamos a frequência de células expressando caspases iniciadoras do processo apoptótico (caspase 9) e caspases efetoras (caspase-3). Adicionalmente avaliamos a liberação citoplasmática de citocromo C mitocondrial, um importante ativador da morte celular programada pela via intrínseca de apoptose. A análise das culturas tratadas na concentração de 200 ?g/mL do EAFPA, apresentou diminuição na expressão de caspase 9 ativa e maior viabilidade dos linfócitos. Por outro lado, nas analises de liberação de citocromo C, cerca de 80% das células analisadas não liberaram a proteina. Não foi observada necrose celular, indicando que outras vias de morte celular possam estar ativadas, levando-nos necessariamente a novas abordagens para caracterização destas vias

Medicinal plants historically have an important role in drug development. Inflammatory and autoimmune disease are increasing in the population, such as diabetes mellitus type 1-A (DM-1A), that stimulates the investigation of the natural compounds therapeutic potential. Experimental models are…

Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Zollner, Ricardo de Lima, 1954-, Pavin, Elizabeth João, Gonçalves, Gisele Mara Silva.

Subjects/Keywords: Passiflora alata; Diabetes Mellitus tipo 1A; Caspase 3; Caspase 9; Apoptose; Passiflora alata; Diabetes Mellitus, Type 1A; Caspase 3; Caspase 9; Apoptosis; Cytochromes c

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APA (6^th Edition):

Carvalho, Virginia Campos, 1. (2019). Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ . (Masters Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504

Chicago Manual of Style (16^th Edition):

Carvalho, Virginia Campos, 1987-. “Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ .” 2019. Masters Thesis, Universidade Estadual de Campinas. Accessed April 22, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504.

MLA Handbook (7^th Edition):

Carvalho, Virginia Campos, 1987-. “Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ .” 2019. Web. 22 Apr 2021.

Vancouver:

Carvalho, Virginia Campos 1. Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ . [Internet] [Masters thesis]. Universidade Estadual de Campinas; 2019. [cited 2021 Apr 22]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504.

Council of Science Editors:

Carvalho, Virginia Campos 1. Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ . [Masters Thesis]. Universidade Estadual de Campinas; 2019. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504

8. 川元, 康嗣. TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.

Degree: 博士(医学), 2016, Nagasaki University / 長崎大学

URL: http://hdl.handle.net/10069/37166

► Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp… (more)

Subjects/Keywords: Anti-TNF-α therapy; Cell death; Caspase 3; Caspase 7; Lung epithelial cells

3 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

川元, �. (2016). TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/37166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

川元, 康嗣. “TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed April 22, 2021. http://hdl.handle.net/10069/37166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

川元, 康嗣. “TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.” 2016. Web. 22 Apr 2021.

Vancouver:

川元 �. TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/10069/37166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

川元 �. TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/37166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

9. Krauss, Amy. Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/29095

► Anthocyanidins induce apoptosis in some cancer cell lines, but studies describing the dose-dependent effects on cell populations are limited. My objective was to evaluate the… (more)

Subjects/Keywords: Anthocyanidins; Delphinidin; Malvidin; Curcumin; Apoptosis; Caspase-3; Caspase-8; HT-29 cells

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APA (6^th Edition):

Krauss, A. (2014). Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krauss, Amy. “Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells.” 2014. Thesis, University of Georgia. Accessed April 22, 2021. http://hdl.handle.net/10724/29095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krauss, Amy. “Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells.” 2014. Web. 22 Apr 2021.

Vancouver:

Krauss A. Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/10724/29095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krauss A. Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Detraz, Morgane. Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities.

Degree: Docteur es, Chimie, 2019, Normandie

URL: http://www.theses.fr/2019NORMR138

►

La qualité du diagnostic oncologique, déterminant la thérapie et le pronostic du patient, repose sur différentes techniques d’imagerie ou modalités, associées à des molécules de… (more)

▼

La qualité du diagnostic oncologique, déterminant la thérapie et le pronostic du patient, repose sur différentes techniques d’imagerie ou modalités, associées à des molécules de contraste afin de générer une image représentative de la situation tumorale et analysable par le praticien. L’imagerie moléculaire, incluant l’imagerie optique de fluorescence et l’imagerie nucléaire, est couramment utilisée pour la prise en charge oncologique des patients. Récemment, une classe hybride de molécules de contraste appelée MOMIA (MOnomolecular Multimodal Imaging Agent) a émergé, associant plusieurs modalités au sein d’une structure moléculaire unique. Cette combinaison performante cumule les forces de chacune des modalités combinées en palliant leur limitation respective. Dans ce cadre, ces travaux de thèse portent sur le développement et l’exploitation d’une plateforme polyvalente bimodale (fluorescente et radioactive) composée d’un synthon peptidique, radiomarquable par chélation du 99mTc, couplé à fluorophore présentant des propriétés spectroscopiques d’émission dans le proche infra-rouge, gamme spectrale privilégiée pour l’imagerie in vivo. Cette plateforme est finalement clickable à toute (bio)molécule d’intérêt, générant à façon des sondes bimodales en une étape de couplage. L’exploitation de cette plateforme bimodale clickable a été explorée dans le contexte du diagnostic oncologique via deux cibles enzymatiques principales : la caspase-3 et les tyrosine-kinases. Les étapes de conception, synthèse, optimisation, développement et validation biologique préliminaire in vitro de la plateforme et des sondes dérivées bimodales sont présentés

Oncologic healthcare and remission prognosis rely on a reliable and accurate diagnosis. Molecular imaging, including optical and nuclear imaging, is currently used for the management cancer therapy. Recently, a new class of bio-imaging agent called MOMIA (MOnomolecular Multimodal Imaging Agent) emerged by combining the synergistic strengths of several modalities on the same molecular structure. We envisioned to merge optical and nuclear imaging in order to develop a molecular tool offering a non-invasive, highly resolutive and sensitive detection. Our approach relies on a universal bimodal clickable scaffold with a selected targeting ligand. Two distinct enzymatic targets have been explored in the oncologic context: caspase-3 as a key component in an apoptotic program and tyrosine kinase inhibitors involved in lung cancer therapy. These multimodal sensors have a promising potential in translational clinical applications.

Advisors/Committee Members: Bohn, Pierre (thesis director), Renard, Pierre-Yves (thesis director).

Subjects/Keywords: Imagerie moléculaire; Multimodalité; Fluorescence; SPECT; Caspase-3; Erlotinib; Molecular imaging; Multimodality; Fluorescence; SPECT; Caspase-3; Erlotinib; 616.075

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Detraz, M. (2019). Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities. (Doctoral Dissertation). Normandie. Retrieved from http://www.theses.fr/2019NORMR138

Chicago Manual of Style (16^th Edition):

Detraz, Morgane. “Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities.” 2019. Doctoral Dissertation, Normandie. Accessed April 22, 2021. http://www.theses.fr/2019NORMR138.

MLA Handbook (7^th Edition):

Detraz, Morgane. “Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities.” 2019. Web. 22 Apr 2021.

Vancouver:

Detraz M. Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities. [Internet] [Doctoral dissertation]. Normandie; 2019. [cited 2021 Apr 22]. Available from: http://www.theses.fr/2019NORMR138.

Council of Science Editors:

Detraz M. Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities. [Doctoral Dissertation]. Normandie; 2019. Available from: http://www.theses.fr/2019NORMR138

11. Rodrigues, Bruna dos Santos. Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3.

Degree: 2013, Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG)

URL: http://repositorio.bc.ufg.br/tede/handle/tede/3726

►

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Made available in DSpace on 2014-12-04T14:18:47Z (GMT). No. of bitstreams: 2 Dissertação - Bruna dos Santos Rodrigues - 2013.pdf: 1797836 bytes, checksum: b56e1b3bdd63eeb9040d2ef050f157f8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-08-30

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES

The death mechanisms induced by a new synthetic compound (4-FTC) in adenocarcinoma prostate cells (PC-3) and its toxicity were investigated in this study. PC-3 cells cytotoxity was evaluated by MTT reduction assay. The mechanisms involved in PC-3 death and cell cycle were investigated by flow cytometry and colorimetric assays. The compound toxicity was analized by cytotoxicity of mononuclear cells (MTT reduction assay) and 3T3 cells (neutral red uptake assay), myelotoxicity, haemolytic activity and acute oral toxicity. 4-FTC has concentration dependent cytotoxic activity in PC-3 cells, and 184,6 μM IC50. Investigation of death mechanisms indicated death by apoptosis, because of the significant increase in phosphatidylserine externalization (109,83%), loss of mytochondrial membrane potential (41,96%), significant increase of DNA fragmentation (284,02%) and capases 3/7 and 9 activity increase, 13,12% and 12,8%, respectively. Furthermore, the treatment of PC-3 cells wih 4-FTC did not induce the reactive oxygen species production, as well as, the induction of acid autophagic vesicles generation and did not change the cell cycle significantly. Althought 4-FTC was able to modulate the expression of some proteins that regulate cell cycle, incresead the expression of p53, p21 and p27. Thus, the results suggests that 4-FTC induced PC-3 death by apoptosis dependent by mitochondrial pathway activation. In toxicity evaluation, 4-FTC presented 52,86 μM and 19,63 μM IC50 to mononuclear and 3T3 cells, respectively; 27,35 μM IC50 to hematopoietic precursors; low acute oral toxicity, classified in GHS category 5, and not significant haemolytic activity.

Neste trabalho, investigaram-se os mecanismos de morte induzidos por um novo composto sintético, 4-fluorbenzaldeidotiossemicarbazona (4-FTC), nas células de adenocarcinoma prostático PC-3 e alguns parâmetros da toxicidade desse composto. A citotoxicidade nas células PC-3 foi avaliada pelo método de redução do MTT, método colorimétrico para avaliar a viabilidade celular. A investigação dos mecanismos de morte…

Advisors/Committee Members: Valadares, Marize Campos, Valadares, Marize Campos, Costa, Nádia do Lago, Cunha, Luiz Carlos da.

Subjects/Keywords: Tiossemicarbazona; Caspase; Apoptose; Célula PC-3; Thiosemicarbazone; Caspase; Apoptosis; PC-3 cells; CIENCIAS DA SAUDE::FARMACIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodrigues, B. d. S. (2013). Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3. (Masters Thesis). Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG). Retrieved from http://repositorio.bc.ufg.br/tede/handle/tede/3726

Chicago Manual of Style (16^th Edition):

Rodrigues, Bruna dos Santos. “Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3.” 2013. Masters Thesis, Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG). Accessed April 22, 2021. http://repositorio.bc.ufg.br/tede/handle/tede/3726.

MLA Handbook (7^th Edition):

Rodrigues, Bruna dos Santos. “Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3.” 2013. Web. 22 Apr 2021.

Vancouver:

Rodrigues BdS. Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3. [Internet] [Masters thesis]. Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG); 2013. [cited 2021 Apr 22]. Available from: http://repositorio.bc.ufg.br/tede/handle/tede/3726.

Council of Science Editors:

Rodrigues BdS. Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3. [Masters Thesis]. Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG); 2013. Available from: http://repositorio.bc.ufg.br/tede/handle/tede/3726

NSYSU

12. Liang, Wei-Zhe. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.

Degree: PhD, Biological Sciences, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

► The effect of the natural essential oil carvacrol or the anesthetic propofol on cell viability, cell cycle distribution, reactive oxygen species (ROS), intracellular free Ca2+… (more)

▼ The effect of the natural essential oil carvacrol or the anesthetic propofol on cell viability, cell cycle distribution, reactive oxygen species (ROS), intracellular free Ca2+ concentrations ([Ca2+]i) and caspase-3-associated apoptosis in human normal cells or non-normal cells is unclear. Human gingival fibroblasts (HGF), human oral cancer cell line (OC2) and human glioblastoma cell line (DRTBG-05MG, HGB) were used in this study. Cell viability was measured by detecting reagent water soluble tetrazolium salt-1 (WST-1). Apoptosis was detected by Annexin V/propidium iodide (PI) staining, cell cycle distribution was detected by PI staining, and ROS was detected by membrane-permeable probe dichlorofluorescein diacetate (DCFH-DA) or hydroethidine (HE) staining. Apoptosis, cell cycle distribution and ROS were analyzed by flow cytometry. The Ca2+-sensitive fluorescent dye fura-2 was applied to measure [Ca2+]i. Caspase-3 expression was detected by western blotting. Carvacrol at 200-800 Î¼M decreased the cell viability of OC2 or HGB cells in a concentration-dependent manner and 1,000 Î¼M carvacrol almost killed all OC2 or HGB cells, but in HGF cells, 200-800 Î¼M carvacrol did not significantly kill cells and 1,000 Î¼M carvacrol decreased only about 63% of cell viability. Similarly, propofol at concentrations between 300 and 600 Î¼M decreased the cell viability of OC2 or HGB cells in a concentration-dependent manner and 700 Î¼M propofol almost killed all OC2 or HGB cells, but in HGF cells, 300-600 Î¼M propofol did not significantly kill cells and 700 Î¼M propofol decreased about 62% of cell viability. In OC2 or HGB cells, carvacrol (200 Î¼M, 400 Î¼M or 600 Î¼M) or propofol (300 Î¼M, 400 Î¼M or 500 Î¼M) induced apoptosis, increased ROS production, evoked cell cycle arrest and activated caspase-3. The caspase-3 inhibitor (DEVD-CHO) partially decreased the apoptotic effect induced by carvacrol or propofol. On the other hand, in OC2 or HGB cells, carvacrol at concentrations between 400 Î¼M and 1,000 Î¼M induced a [Ca2+]i rise in a concentration-dependent manner and the signal was reduced by removal of extracellular Ca2+. In HGF cells, carvacrol at 1000 Î¼M did not induce immediate [Ca2+]i rises in Ca2+-containing or Ca2+-free medium. Similarly, propofol at concentrations between 400 Î¼M and 1,000 Î¼M induced a [Ca2+]i rise in a concentration-dependent manner in OC2 or HGB cells, but not in HGF cells. This cytotoxic effect was not reversed in carvacrol-treated groups, but was partially reversed in propofol-treated groups when cytosolic Ca2+ was chelated with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA-AM) in OC2 or HGB cells. The apoptotic effect of propofol was also partially decreased by BAPTA-AM treatment in OC2 and HGB cells. In OC2 and HGB cells, carvacrol- or propofol-induced Ca2+ signal was not altered by L-type voltage-gated Ca2+ channel blocker nifedipine, store-operated Ca2+ channel blocker econazole or SK&F96365) and protein kinase C (PKC) activator phorbol myristate acetate (PMA), but… Advisors/Committee Members: Nai-Wen Tsai (chair), Chung-Ren Jan (chair), Ko-Long Lin (chair), Cheng-Hsien Lu (committee member), Yao-Dung Hsieh (chair).

Subjects/Keywords: Apoptosis; cell cycle; ROS; Ca2+; Caspase-3; Propofol; Carvacrol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liang, W. (2012). Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

Chicago Manual of Style (16^th Edition):

Liang, Wei-Zhe. “Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.” 2012. Doctoral Dissertation, NSYSU. Accessed April 22, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617.

MLA Handbook (7^th Edition):

Liang, Wei-Zhe. “Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.” 2012. Web. 22 Apr 2021.

Vancouver:

Liang W. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. [Internet] [Doctoral dissertation]. NSYSU; 2012. [cited 2021 Apr 22]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617.

Council of Science Editors:

Liang W. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. [Doctoral Dissertation]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

University of Miami

13. Gillespie, Megan Marie. Improving Sensing Through the Design of Recombinant Proteins.

Degree: PhD, Chemistry (Arts and Sciences), 2015, University of Miami

URL: https://scholarlyrepository.miami.edu/oa_dissertations/1460

► Genetic engineering has provided us with the ability to advance medicine and technology by creating proteins that can be used for various applications. Two such… (more)

Subjects/Keywords: protein engineering; apoptosis; cornea; caspase-3; aequorin; glucose oxidase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gillespie, M. M. (2015). Improving Sensing Through the Design of Recombinant Proteins. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/1460

Chicago Manual of Style (16^th Edition):

Gillespie, Megan Marie. “Improving Sensing Through the Design of Recombinant Proteins.” 2015. Doctoral Dissertation, University of Miami. Accessed April 22, 2021. https://scholarlyrepository.miami.edu/oa_dissertations/1460.

MLA Handbook (7^th Edition):

Gillespie, Megan Marie. “Improving Sensing Through the Design of Recombinant Proteins.” 2015. Web. 22 Apr 2021.

Vancouver:

Gillespie MM. Improving Sensing Through the Design of Recombinant Proteins. [Internet] [Doctoral dissertation]. University of Miami; 2015. [cited 2021 Apr 22]. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1460.

Council of Science Editors:

Gillespie MM. Improving Sensing Through the Design of Recombinant Proteins. [Doctoral Dissertation]. University of Miami; 2015. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1460

University of Toronto

14. Latif, Maya. Regulation of Cisplatin-induced Programmed Cell Death In Vivo.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/82403

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Cisplatin [ cis-PtCl2 NH3)2 ] is a widely utilized chemotherapeutic agent. Its proposed mechanism of action involves DNA-platinum adduct formation that triggers DNA damage responses,… (more)

Subjects/Keywords: Apoptosis; Caspase-3; Cisplatin; DNA damage; Necroptosis; PCD; 0307

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Latif, M. (2016). Regulation of Cisplatin-induced Programmed Cell Death In Vivo. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/82403

Chicago Manual of Style (16^th Edition):

Latif, Maya. “Regulation of Cisplatin-induced Programmed Cell Death In Vivo.” 2016. Masters Thesis, University of Toronto. Accessed April 22, 2021. http://hdl.handle.net/1807/82403.

MLA Handbook (7^th Edition):

Latif, Maya. “Regulation of Cisplatin-induced Programmed Cell Death In Vivo.” 2016. Web. 22 Apr 2021.

Vancouver:

Latif M. Regulation of Cisplatin-induced Programmed Cell Death In Vivo. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/1807/82403.

Council of Science Editors:

Latif M. Regulation of Cisplatin-induced Programmed Cell Death In Vivo. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/82403

Laurentian University

15. Albalawi, Ghadah. The effect of andrographis paniculata on the growth of malignant cancer cells .

Degree: 2016, Laurentian University

URL: https://zone.biblio.laurentian.ca/handle/10219/2674

► There are a variety of plants that have been recognized and used in traditional medicine for their health benefits. Among these plants is Andrographis paniculata,… (more)

Subjects/Keywords: Andrographis paniculata; malignant cancer cells; Apoptosis; Caspase 3,; Sub-G1

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APA (6^th Edition):

Albalawi, G. (2016). The effect of andrographis paniculata on the growth of malignant cancer cells . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/handle/10219/2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells .” 2016. Thesis, Laurentian University. Accessed April 22, 2021. https://zone.biblio.laurentian.ca/handle/10219/2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells .” 2016. Web. 22 Apr 2021.

Vancouver:

Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells . [Internet] [Thesis]. Laurentian University; 2016. [cited 2021 Apr 22]. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells . [Thesis]. Laurentian University; 2016. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University

16. Harding, Charles P. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.

Degree: MS, Biological and Irrigation Engineering, 2019, Utah State University

URL: https://digitalcommons.usu.edu/etd/7436

► Muscle loss from lack of activity is a serious issue for immobilized patients on Earth and in human spaceflight, where the low gravity environment… (more)

Subjects/Keywords: MuRF1; MAFbx; Caspase 3; C2C12; RCCS; Biological Engineering

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APA (6^th Edition):

Harding, C. P. (2019). In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/7436

Chicago Manual of Style (16^th Edition):

Harding, Charles P. “In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.” 2019. Masters Thesis, Utah State University. Accessed April 22, 2021. https://digitalcommons.usu.edu/etd/7436.

MLA Handbook (7^th Edition):

Harding, Charles P. “In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.” 2019. Web. 22 Apr 2021.

Vancouver:

Harding CP. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. [Internet] [Masters thesis]. Utah State University; 2019. [cited 2021 Apr 22]. Available from: https://digitalcommons.usu.edu/etd/7436.

Council of Science Editors:

Harding CP. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. [Masters Thesis]. Utah State University; 2019. Available from: https://digitalcommons.usu.edu/etd/7436

17. Ribeiro, Roana Cecília dos Santos. Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia.

Degree: 2019, Universidade Estadual Paulista (UNESP)

URL: http://hdl.handle.net/11449/181062

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O carcinoma de células escamosas (CCE) cutâneo é uma neoplasia maligna, que representa 15% dos tumores de pele em felinos. As regiões mais acometidas nos felinos pelo CCE são o plano nasal, pálpebras e pavilhões auriculares, tornando desafiadora a excisão cirúrgica com amplas margens. Desta forma, novas abordagens terapêuticas para o CCE cutâneo estão sendo investigadas, com destaque para a eletroquimioterapia (EQT). A determinação das taxas de proliferação celular e apoptose tumoral por meio da imuno-histoquímica, permite avaliar o comportamento biológico tumoral, com otimização da evolução clínica do paciente. Este trabalho teve como objetivo analisar as expressões imuno-histoquímicas de Ki-67, COX-2 e caspase-3 e correlacioná-las com os aspectos clínico-patológicos e o tipo de resposta à EQT em CCE cutâneo de felinos; assim, determinar o potencial prognóstico e/ou preditivo destas variáveis. Para tanto, foram avaliadas 13 amostras de CCE cutâneo de felinos antes da EQT e as análises estatísticas quanto à intensidade de correlação entre as variáveis foram realizadas utilizando o coeficiente de correlação de Spearman, com nível de significância de 95%. Os resultados indicam que houve correlação positiva significativa entre o grau histopatológico e o grau de pleomorfismo celular (ρ=0,81, p<0,01), correlação negativa significativa entre o grau histopatológico e a resposta à EQT (ρ= - 0,6; p=0,03), correlação positiva significativa entre o tamanho e o estadiamento tumoral…

Advisors/Committee Members: Universidade Estadual Paulista (UNESP), De Nardi, Andrigo Barboza [UNESP].

Subjects/Keywords: Caspase-3; COX-2; Ki-67; Valor preditivo; Valor prognóstico

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APA (6^th Edition):

Ribeiro, R. C. d. S. (2019). Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia. (Masters Thesis). Universidade Estadual Paulista (UNESP). Retrieved from http://hdl.handle.net/11449/181062

Chicago Manual of Style (16^th Edition):

Ribeiro, Roana Cecília dos Santos. “Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia.” 2019. Masters Thesis, Universidade Estadual Paulista (UNESP). Accessed April 22, 2021. http://hdl.handle.net/11449/181062.

MLA Handbook (7^th Edition):

Ribeiro, Roana Cecília dos Santos. “Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia.” 2019. Web. 22 Apr 2021.

Vancouver:

Ribeiro RCdS. Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia. [Internet] [Masters thesis]. Universidade Estadual Paulista (UNESP); 2019. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/11449/181062.

Council of Science Editors:

Ribeiro RCdS. Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia. [Masters Thesis]. Universidade Estadual Paulista (UNESP); 2019. Available from: http://hdl.handle.net/11449/181062

18. Leipelt, Juliano. Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais.

Degree: 2016, Brazil

URL: http://hdl.handle.net/10737/1110&

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O câncer é apontado como a segunda maior causa de morte em todo o mundo, com previsão de em breve ser tornar a primeira. O câncer de próstata está entre os 5 tipos de câncer mais diagnosticados em homens, sendo que o câncer hepático está em segundo lugar em taxa de mortalidade entre homens e mulheres. Indícios apontam para uma ativação das vias da inflamação associados a uma inibição das vias de morte celular no processo de carcinogênese. A regulação destas vias torna-se alvo importante e complementar no controle do câncer, sendo estimulada a busca de biomoléculas com este potencial. As plantas são importante fonte de descoberta de novas biomoléculas com ampla utilização para o tratamento de diversas patologias. A família Myrtaceae possui diversas espécies que são apontadas como fortes candidatos em potencial nesta busca, incluindo as do gênero Myrciaria. A espécie Myrciaria plinioides não possui estudos referentes suas propriedades terapêuticas ou a atuação em vias de sinalização envolvidas na inflamação ou na carcinogênese. Neste contexto, este estudo teve por objetivo avaliar a atividade do extrato etanólico de M. plinioides em células de carcinoma hepatocelular (HepG2) e próstata (LNCaP) , através da análise de expressão dos marcadores p38-α, pp38-α, NF-κB e caspase-3, envolvidos na carcinogênese, e o efeito sobre a viabilidade celular através do método de MTT. A viabilidade das células foi alterada significativamente, em ambas as linhagens celulares quando tratadas com o extrato etanólico. A análise da expressão proteica demonstra significativa inibição da expressão de p38-α e caspase-3 nas células LNCaP, quando tratadas com extrato etanólico de M. plinioides seguido de LPS. Em células HepG2, somente houve alteração na expressão da caspase-3 na concentração de 200 μg/mL, com ou sem adição de LPS após tratamento com extrato. Os resultados deste estudo demonstraram redução da viabilidade celular nas duas linhagens tumorais, expressão diferenciada de proteínas envolvidas em apoptose, o que leva a indícios da ativação de mecanismos distintos pelo extrato em cada tipo celular. Estudos futuros para averiguar o mecanismo celular e a indução de morte em células tumorais de câncer de próstata e…

Subjects/Keywords: LNCaP; HepG2; p38α; pp38α; NF-Kb; Caspase-3

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leipelt, J. (2016). Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais. (Masters Thesis). Brazil. Retrieved from http://hdl.handle.net/10737/1110&

Chicago Manual of Style (16^th Edition):

Leipelt, Juliano. “Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais.” 2016. Masters Thesis, Brazil. Accessed April 22, 2021. http://hdl.handle.net/10737/1110&.

MLA Handbook (7^th Edition):

Leipelt, Juliano. “Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais.” 2016. Web. 22 Apr 2021.

Vancouver:

Leipelt J. Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais. [Internet] [Masters thesis]. Brazil; 2016. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/10737/1110&.

Council of Science Editors:

Leipelt J. Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais. [Masters Thesis]. Brazil; 2016. Available from: http://hdl.handle.net/10737/1110&

Queensland University of Technology

19. Burgess, Joshua T. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.

Degree: 2016, Queensland University of Technology

URL: http://eprints.qut.edu.au/98661/

► SASH1 (SAM and SH3 domain-containing protein 1) is a putative tumour suppressor functioning in the control of apoptosis and cellular proliferation. In summary this project… (more)

Subjects/Keywords: SASH1; Caspase 3; Chloropyramine; NF-κ; B; DNA Damage repair; hSSB1; Apoptosis; Breast Cancer; Lung Cancer; 14-3-3

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burgess, J. T. (2016). The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/98661/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burgess, Joshua T. “The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.” 2016. Thesis, Queensland University of Technology. Accessed April 22, 2021. http://eprints.qut.edu.au/98661/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burgess, Joshua T. “The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.” 2016. Web. 22 Apr 2021.

Vancouver:

Burgess JT. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. [Internet] [Thesis]. Queensland University of Technology; 2016. [cited 2021 Apr 22]. Available from: http://eprints.qut.edu.au/98661/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burgess JT. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. [Thesis]. Queensland University of Technology; 2016. Available from: http://eprints.qut.edu.au/98661/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Ol, Kevser Kuşat. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması .

Degree: ESOGÜ, Tıp Fakültesi, Tıbbi Biyokimya, 2014, Eskisehir Osmangazi University

URL: http://hdl.handle.net/11684/892

► Bu çalışmada modifiye sıvı diyet aracılığıyla prenatal ve postnatal etanol maruziyeti oluşturulan rat yavrularının serebral korteksindeki nörodejenerasyonun etkisini incelemeyi ve betain ve omega-3 takviyesinin koruyucu… (more)

▼ Bu çalışmada modifiye sıvı diyet aracılığıyla prenatal ve postnatal etanol maruziyeti oluşturulan rat yavrularının serebral korteksindeki nörodejenerasyonun etkisini incelemeyi ve betain ve omega-3 takviyesinin koruyucu etkilerini göstermeyi amaçladık. Prenatal ve postnatal periyodda etanole maruz kalındığında yavrularda Fetal Alkol Sendromu (FAS) görülmektedir. Ratlar kontrol, etanol, etanol+omega-3, etanol+betain, etanol+betain+omega-3 şeklinde 5 gruba ayrıldı. Rat yavrularının serebral korteksindeki etanol indüksiyonuna bağlı değişiklerde betain ve omega-3 ün etkilerini incelemek amacıyla biyokimyasal olarak sitokrom c, kaspaz-3, kalpain, katepsin B ve L, DNA fragmantasyonu seviyeleri incelenmiş histolojik ve morfometrik bulgular elde edilmiştir. Etanol grubunda kaspaz-3, kalpain, katepsin B, sitokrom c seviyeleri kontrol grubu ile karşılaştırıldığında belirgin derecede artmıştır. Dahası, kaspaz-3 ve kalpain seviyeleri etanol+omega-3, etanol+betain, etanol+betain+omega-3 gruplarında etanol grubuyla karşılaştırıldığında azalmıştır. Aynı zamanda katepsin B düzeyleri etanol+betain grubunda kontrolden daha yüksektir. Etanol+betain+omega-3 grubunda katepsin B etanol ve etanol+betain gruplarıyla karşılaştırıldığında azalmıştır. Katepsin L ve DNA fragmantasyonunda istatistiksel olarak farklılık bulunamamıştır. Doku hasarı değerlendirmesi histolojik açıdan; kontrol, etanol+omega-3, etanol+betain, etanol+betain+omega-3 gruplarında hemoraji, PMNL, mikroglia etanol grubuyla karşılaştırıldığında istatistiksel olarak düşüktür. Aynı zamanda etanol+omega-3 ve kontrol gruplarında konjesyon ve nekroz etanol indüklü hasara göre azalış göstermiştir. Morfometrik analiz sonucu etanol alınımı etanol+omega-3 ve kontrol gruplarına göre nekrotik hücre sayısını artırmıştır. Sonuç olarak, postnatal etanol maruziyeti rat beyinlerinde nekrotik hücre ölümünü tetiklemektedir, omega-3 ve betain nörodejenerasyonu azaltmaktadır. Omega-3 ve betain özellikle FAS ın önlenmesinde, nörodejenerasyon açısından yararlı olabilir. Advisors/Committee Members: Kanbak, Güngör (advisor).

Subjects/Keywords: Betain; Fetal Alkol Sendromu (FAS); Kaspaz-3; Omega-3; Sitokrom c; Caspase-3; Betaine; Cytochrome c; Fetal Alcohol Syndrome (FAS)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ol, K. K. (2014). Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması . (Thesis). Eskisehir Osmangazi University. Retrieved from http://hdl.handle.net/11684/892

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ol, Kevser Kuşat. “Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması .” 2014. Thesis, Eskisehir Osmangazi University. Accessed April 22, 2021. http://hdl.handle.net/11684/892.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ol, Kevser Kuşat. “Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması .” 2014. Web. 22 Apr 2021.

Vancouver:

Ol KK. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması . [Internet] [Thesis]. Eskisehir Osmangazi University; 2014. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/11684/892.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ol KK. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması . [Thesis]. Eskisehir Osmangazi University; 2014. Available from: http://hdl.handle.net/11684/892

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Johannes Gutenberg Universität Mainz

21. Golbs, Antje. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.

Degree: 2008, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/

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Die Apoptose spielt eine entscheidende Rolle während der normalen Entwicklung des zentralen Nervensystems. Elektrische Aktivität und die Versorgung mit trophischen Faktoren sind ausschlaggebend für das… (more)

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Die Apoptose spielt eine entscheidende Rolle während der normalen Entwicklung des zentralen Nervensystems. Elektrische Aktivität und die Versorgung mit trophischen Faktoren sind ausschlaggebend für das Überleben von Neuronen. Um zu untersuchen, welche zellulären Prozesse die aktivitätsabhängige Apoptose in organotypischen Schnittkulturen des neugeborenen Neokortex beeinflussen, wurde in der vorliegenden Arbeit immunzytochemisch das Auftreten aktivierter Caspase-3, nach pharmakologischer Beeinflussung von Ionenkanälen und membranständigen Rezeptoren analysiert. Die Unterdrückung neuronaler Aktivität durch den Natriumionenkanalblocker TTX führte zu einem signifikanten Verlust kortikaler Neuronen. Ein ähnlicher Anstieg der Zahl apoptotischer Neurone konnte durch Applikation von Antagonisten ionotroper Glutamatrezeptoren, GABAA-Rezeptoren oder neuronaler Gap Junctions induziert werden. Jedoch konnte bei einigen Antagonisten die apoptosefördernde Wirkung erst nach längerer Einwirkung beobachtet werden. Im Weiteren wurde eine Methode etabliert, mit deren Hilfe eine Echtzeitanalyse der Apoptose kortikaler Neurone unter dem Entzug trophischer Faktoren in Gegenwart unterschiedlicher extrazellulärer Kaliumkonzentrationen ermöglicht wurde. Dazu wurden dissoziierte kortikale Kulturen mit dem pCaspase3-sensor Vektor transfiziert. Das durch dieses Plasmid codierte fluoreszente Protein wird Caspase-3 abhängig gespalten. In der vorliegenden Arbeit konnte gezeigt werden, dass der Caspase3-sensor spezifisch für die Aktivierung der Caspase-3 ist, und dass die Überlebensfähigkeit der transfizierten Neurone durch das Transfektionsprotokoll nicht beeinflusst wird.

Apoptosis is an essential process during normal development of the brain. Electrical activity and supply with survival factors play a major role in regulating neuronal survival. I aimed at correlating neuronal activity and apoptosis in the developing murine neocortex. Therefore organotypic slice cultures of newborn mouse cerebral cortex were treated with antagonists of different ligand-gated receptors and voltage-dependent ion channels. Apoptosis was assessed using an antibody specific for the activated form of caspase-3. Blocking network activity by the sodium channel blocker TTX caused a significant loss of cortical neurons. Moreover application of antagonists of ionotropic glutamate receptors, GABAA receptors or neuronal gap junctions resulted in a similar increase in the number of apoptotic neurons. However some antagonists increased neuronal loss only after prolonged exposure. In a further approach an assay was established which involves the transfection of dissociated cultures of the newborn mouse neocortex with the pCaspase3-sensor vector encoding a protein sensitive to cleavage by caspase-3. With this assay the impact of different extracellular potassium concentrations on caspase-3 dependent cortical apoptosis following trophic deprivation should be elucidated. The method was found to be specific for caspase 3 activation and did not interfere with the viability of…

Subjects/Keywords: Apoptose, elektrische Aktivität, Caspase-3, Neuronen, neuronale Entwicklung, Neokortex, Neurotrophine, Calcium, Kalium; apoptosis, electrical activity, caspase-3, neuron, neuronal development, neocortex, neurotrophins, calcium, potassium; Natural sciences and mathematics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Golbs, A. (2008). Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/

Chicago Manual of Style (16^th Edition):

Golbs, Antje. “Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.” 2008. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed April 22, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/.

MLA Handbook (7^th Edition):

Golbs, Antje. “Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.” 2008. Web. 22 Apr 2021.

Vancouver:

Golbs A. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2008. [cited 2021 Apr 22]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/.

Council of Science Editors:

Golbs A. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2008. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/

22. Raquel Autran Coelho. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.

Degree: 2008, Universidade Federal de São Paulo

URL: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563

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Objetivos: Estudar a expressao imuno-histoquimica de topoisomerase II e de caspase-3 ativada, marcadores de proliferacao e de apoptose, respectivamente, a deteccao de DNA HPV e… (more)

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Objetivos: Estudar a expressao imuno-histoquimica de topoisomerase II e de caspase-3 ativada, marcadores de proliferacao e de apoptose, respectivamente, a deteccao de DNA HPV e a evolucao da lesao cervical em mulheres portadoras de lesao intra-epitelial escamosa de baixo grau (LBG). Metodos: Foram avaliadas 40 mulheres portadoras de LBG e 32 sem neoplasia cervical, diagnosticadas por exame cito-colpo-histopatologico, quanto a imunoexpressao de topoisomerase II e de caspase-3 ativada e quanto a deteccao de DNA HPV por PCR consensual (GP5+/GP6+) em material de esfregaco cervico-vaginal. Os achados foram relacionados as variaveis clinicas das pacientes e a evolucao clinica das lesoes cervicais em 12 meses. As pacientes assinaram termo de consentimento livre e esclarecido. Resultados: A media percentual de celulas imunomarcadas por topoisomerase foi de 11,71% e 4,13%, no grupo com LBG e controle, respectivamente, com diferenca estatisticamente significante. Observou-se que houve expressao de caspase-3 em 17 (42,5%) e em 5 (15,63%) pacientes com e sem LBG, respectivamente, com diferenca estatisticamente significante. Foi detectado HPV DNA em 65% das pacientes com LBG e em 59,4% das pacientes sem lesao cervical, sem relacao com a expressao de topoisomerase II ou caspase-3. Na presenca de DNA-HPV, a expressao de topoisomerase II no grupo com LBG foi significativamente maior do que em fragmentos sem lesao. Nao foi observada diferenca quanto a evolucao da lesao cervical em 12 meses de acordo com a imunoexpressao de topoisomerase II. Com relacao a caspase-3 ativada, a maioria das pacientes com imuno-histoquimica negativa teve regressao da lesao cervical. Conclusoes: A imunoexpressao de topoisomerase II e de caspase-3 ativada podem ser considerados marcadores de proliferacao e de apoptose em lesao cervical de baixo grau, sem relacao com a presenca de DNA-HPV.

Purpose: To evaluate the correlation between the expression of topoisomerase II alpha, active caspase-3 and infection with human papillomavirus in low-grade cervical intraepithelial lesion and in the normal cervix, and whether they might influence susceptibility to, or evolution of, cervical lesion. Patients and methods: Forty cervical biopsies patients with low-grade cervical intraepithelial lesion and thirty-two with normal cervix were stained by immunohistochemistry for topoisomerase IIá and active caspase-3 and were investigated for the presence of HPV on exfoliated cells by general primer GP5+/6+ PCR amplification of DNA. These findings were correlated with clinicopathological features of the patients including their clinical outcome after twelve months. Subjects provided written informed consent. Results: Low-grade CIN patients as a group had a significantly higher expression of topoisomerase II alpha compared to controls, without correlation to disease outcome at 12 months. Caspase-3 was expressed in 42.5% of CIN patients and in 15.63% without disease, and most of women without caspase-3 receded cervical lesion. HPV DNA testing was positive in 65% of the patients…

Advisors/Committee Members: Ismael Dale Cotrim Guerreiro da Silva, Neila Maria de Góis Speck, Gustavo Rubino de Azevedo Focchi, Julisa Chamorro Lascasas Ribalta, José Eleutério Junior, Roberto Zamith.

Subjects/Keywords: DNA topoisomerases tipo II; Caspase 3; Neoplasia intraepitelial cervical; Infecções por papilomavírus; GINECOLOGIA E OBSTETRICIA; DNA topoisomerases type II; Caspase 3; Cervical intraepithelial neoplasia; Papillomavirus infections

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Coelho, R. A. (2008). Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. (Thesis). Universidade Federal de São Paulo. Retrieved from http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coelho, Raquel Autran. “Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.” 2008. Thesis, Universidade Federal de São Paulo. Accessed April 22, 2021. http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coelho, Raquel Autran. “Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.” 2008. Web. 22 Apr 2021.

Vancouver:

Coelho RA. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. [Internet] [Thesis]. Universidade Federal de São Paulo; 2008. [cited 2021 Apr 22]. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coelho RA. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. [Thesis]. Universidade Federal de São Paulo; 2008. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Champagne, Julien. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.

Degree: Docteur es, Biologie Santé, 2018, Montpellier

URL: http://www.theses.fr/2018MONTT018

► Chez la femme, le cancer du sein est le cancer le plus fréquemment diagnostiqué. Différents traitements sont disponibles selon le sous-type tumoral. Cependant, certaines patientes… (more)

▼ Chez la femme, le cancer du sein est le cancer le plus fréquemment diagnostiqué. Différents traitements sont disponibles selon le sous-type tumoral. Cependant, certaines patientes sont réfractaires à ces thérapies et restent vulnérables lors de récidives. Le cancer a longtemps été défini par une division aberrante des cellules, mais aujourd'hui, il est évident que la résistance à la mort cellulaire programmée est un paramètre majeur dans l'étiologie de la maladie.Les cyclines de type D régulent le cycle cellulaire en permettant la transition de la phase G1 à la phase S. Pour cela, elles activent les kinases dépendantes des cyclines 4/6 (CDK4/6) qui phosphorylent les protéines du rétinoblastome ce qui libère le facteur de transcription E2F. La Cycline D1 (CycD1) nucléaire est donc centrale dans le contrôle du cycle. Son gène est amplifié dans les cancers humains et la moitié des patientes atteintes d'un cancer du sein ont une surexpression de CycD1. Par l’activation de CDK4, CycD1 est essentielle à l'apparition et à la progression tumorale. Ainsi, des inhibiteurs spécifiques de CDK4/6 ont été développés contre le cancer du sein. Malheureusement, certaines patientes restent insensibles à ce traitement. À ce titre, le ciblage spécifique de CycD1 pourrait représenter une alternative clinique. En effet, en plus de la régulation du cycle, CycD1 est également impliquée, indépendamment de CDK4, dans la survie des cellules cancéreuses. Cependant, aucun mécanisme de l'impact de CycD1 dans le maintien tumoral n'a été établi pour démontrer ce potentiel thérapeutique. En outre, CycD1 a été décrite dans les organes à l’âge adulte pour réguler le métabolisme du glucose et l'hématopoïèse. Par conséquent, pour éviter tout effet secondaire indésirable, nous avons décidé d’évaluer l’implication potentielle de CycD1 dans les organes adultes. Grâce au Tandem-HTRF, basé sur le transfert d'énergie entre deux anticorps, nous avons révélé la dynamique inattendue de CycD1 dans chaque organe adulte. De plus, nous avons montré que l’altération de l'expression de CycD1 conduit à une diminution des capacités de survie des cellules saines post-mitotiques.Au vu de ces limitations, nous avons développé une nouvelle approche d'ARN interférence spécifique des cellules cancéreuses appelée TAG-RNAi. Cette technologie permet de cibler CycD1 uniquement dans la tumeur afin d'épargner les cellules saines. Cette approche innovante consiste à cibler un tag présent uniquement sur l’ARNm de CycD1 des cellules cancéreuses. Ainsi, nous avons découvert que le ciblage spécifique de CycD1 induit une régression rapide et spontanée des tumeurs dépendantes des oncogènes RAS ou ERBB2. Par protéomique in vivo, j'ai découvert que lors de stress pro-apoptotiques, CycD1 cytoplasmique interagit avec la procaspase-3 et bloque son activation pour empêcher l'apoptose des cellules. Ces travaux démontrent la valeur clinique du ciblage spécifique de CycD1 dans les cancers afin d'améliorer l'efficacité des chimiothérapies.Par conséquent, il restait à déterminer comment appliquer le… Advisors/Committee Members: Pin, Jean-Philippe (thesis director).

Subjects/Keywords: Cancer du sein; Cycline D1; Apoptose; Caspase-3; Thérapie à la carte; ARN interférence; Breast cancer; Cyclin D1; Apoptosis; Caspase-3; Personalized medecine; RNA interference

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Champagne, J. (2018). Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2018MONTT018

Chicago Manual of Style (16^th Edition):

Champagne, Julien. “Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.” 2018. Doctoral Dissertation, Montpellier. Accessed April 22, 2021. http://www.theses.fr/2018MONTT018.

MLA Handbook (7^th Edition):

Champagne, Julien. “Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.” 2018. Web. 22 Apr 2021.

Vancouver:

Champagne J. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. [Internet] [Doctoral dissertation]. Montpellier; 2018. [cited 2021 Apr 22]. Available from: http://www.theses.fr/2018MONTT018.

Council of Science Editors:

Champagne J. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. [Doctoral Dissertation]. Montpellier; 2018. Available from: http://www.theses.fr/2018MONTT018

24. Maria Zilah Benetone. Apoptose e proliferação na placenta de búfalas.

Degree: 2005, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/

► A apoptose é um processo fisiológico que desempenha papel crucial no desenvolvimento, remodelagem e senescência teciduais, inclusive placentários. A placenta, enquanto órgão temporário, atravessa todas… (more)

▼ A apoptose é um processo fisiológico que desempenha papel crucial no desenvolvimento, remodelagem e senescência teciduais, inclusive placentários. A placenta, enquanto órgão temporário, atravessa todas estas fases em aproximadamente 10 meses, na espécie bubalina. O crescimento da placenta e a nutrição fetal requerem altas taxas de renovação e diferenciação celulares, e a maturação placentária está relacionada à redução das células epiteliais das criptas carunculares maternas. As modificações morfológicas celulares decorrentes do processo de apoptose são fruto de eventos bioquímicos complexos promovidos por uma família de cisteína-proteases, as caspases, especialmente as caspases executoras, dentre as quais se destaca a caspase-3, capaz de degradar várias proteínas citoplasmáticas e nucleares. Durante a apoptose, ocorre a clivagem caspase-mediada da citoqueratina 18, proteína dos filamentos intermediários do citoesqueleto, e com isso a formação de um neoepítopo específico. Por meio de métodos imunoistoquímicos pode-se detectar a presença tanto deste neoepítopo, quanto da forma ativa da caspase-3, o que demonstra que a célula entrou em estágio irreversível de morte celular. Morfologicamente, algumas das principais alterações celulares observadas são condensação da cromatina, a degradação e fragmentação do DNA, a formação de ?blebs? (pregas/bolhas) na membrana plasmática, além da fragmentação celular em corpúsculos apoptóticos, os quais podem ser identificados em cortes corados pelo método de rotina hematoxilina e eosina, utilizando-se microscópio de imunofluorescência, devido à eosinofluorescência das células em apoptose. Assim como a apoptose, a proliferação celular participa no equilíbrio homeostático tissular. Neste estudo, pretende-se avaliar a ocorrência de apoptose e proliferação celular em 42 placentônios de diferentes animais em diversas fases gestacionais (2-10 meses de gestação), em tecidos fixados em 4% paraformoldeído, incluídos em paraplast e submetidos à imunoistoquímica (anticorpo monoclonal M30 CytoDeath; Caspase-3 Clivada; PCNA - antígeno de marcação nuclear), sendo também avaliada a presença de corpúsculos apoptóticos eosinofluorescentes nas amostras. Utilizando-se M30 e caspase-3 clivada pudemos constatar a ocorrência de apoptose nos epitélios uterino e trofoblástico, em células gigantes placentárias e, ocasionalmente, em células mesenquimais fetais, do estroma uterino e endoteliais. Não houve diferenças significativas (p<0.05) entre os métodos adotados, mas sim entre os estágios gestacionais. Para o M30, houve um aumento significante da apoptose do primeiro grupo (2-4.5 meses) em relação ao quarto grupo (9-10 meses); no caso da Caspase-3 Clivada houve um aumento estatísticamente significante (p<0.05) entre os três primeiros grupos (2-4.5; 5-6.5; e 7-8.5 meses de gestação, respectivamente) de animais em relação ao quarto grupo. Para o PCNA, ocorreu uma diminuição no número de células em proliferação dos dois primeiros grupos de animais em relação ao quarto grupo (p<0.05). A presença de corpúsculos… Advisors/Committee Members: Maria Angélica Miglino, Hiro Goto, José Luiz Guerra.

Subjects/Keywords: Apoptose; Búfalos; Caspase-3; Placenta; Proliferação; Apoptosis; Buffalo; Caspase 3; Placenta; Proliferation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benetone, M. Z. (2005). Apoptose e proliferação na placenta de búfalas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/

Chicago Manual of Style (16^th Edition):

Benetone, Maria Zilah. “Apoptose e proliferação na placenta de búfalas.” 2005. Masters Thesis, University of São Paulo. Accessed April 22, 2021. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/.

MLA Handbook (7^th Edition):

Benetone, Maria Zilah. “Apoptose e proliferação na placenta de búfalas.” 2005. Web. 22 Apr 2021.

Vancouver:

Benetone MZ. Apoptose e proliferação na placenta de búfalas. [Internet] [Masters thesis]. University of São Paulo; 2005. [cited 2021 Apr 22]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/.

Council of Science Editors:

Benetone MZ. Apoptose e proliferação na placenta de búfalas. [Masters Thesis]. University of São Paulo; 2005. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/

NSYSU

25. Chou, Chiang-Ting. Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline.

Degree: PhD, Biological Sciences, 2008, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559

► Depression is a physiological disorder that may be treated by increasing the bodyâs amount of one or a few of the following neurotransmitters: serotonin, dopamine… (more)

▼ Depression is a physiological disorder that may be treated by increasing the bodyâs amount of one or a few of the following neurotransmitters: serotonin, dopamine and norepinephrine. Although there are seven distinct classes of antidepressants, selective serotonin reuptake inhibitors (SSRIs) and tetracyclic antidepressants are widely prescribed and generally regarded as the first-line drugs in the treatment of depression. However, many physiological roles of some SSRIs appear to be dissociated with the inhibition of serotonin reuptake. For instance, paroxetine, a member of SSRIs and maprotiline, a member of tetracyclic antidepressant, have been shown to induce apoptosis or to prevent other agents from inducing apoptosis in several cell lines. Thus the effects of these two drugs on the apoptosis are still controversial. The aim of this study is to investigate the molecular mechanisms of paroxetine and maprotiline in induction of cell death in human osteosarcoma and murine neuroblastoma cells. First, WST-1 reduction assays and propidium iodide-staining assays were used to determine cell viability and apoptosis in the presence of paroxetine and maprotiline. Then immunoblotting was used to measure the activity of apoptotic markers caspase-3 and mitogen-activated protein kinases (MAPKs) to survey the apoptotic pathways induced by these two antidepressants. The experimental results may be helpful to understand the pharmacological and toxicological effects of these two antidepressants in cells from important organs. Results showed that paroxetine caused apoptosis via the activation of caspase-3 in cultured human osteosarcoma cells (MG63). Although paroxetine could activate the phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK), only SB203580 (a p38 MAPK inhibitor) partially prevented cells from apoptosis. Paroxetine was also found to induce [Ca2+]i increases but pretreatment with BAPTA/AM, a Ca2+ chelator, prevented paroxetine-induced [Ca2+]i increases, and thus did not protect cells from death. These results suggest that paroxetine caused Ca2+-independent apoptosis via the activation of p38 MAPK-associated caspase-3 in MG63 cells. Maprotiline was also found to induce apoptosis through increased caspase-3 activation in murine neuroblastoma Neuro-2a cells. Induction of JNK phosphorylation contributed to the activation of caspase-3 resulting in maprotiline-induced Neuro-2a cell apoptosis. Thus, it appears that maprotiline induced apoptosis via JNK/caspase-3-dependent signaling pathways. Blockage of activation of ERK was found to increase the activation of caspase-3 leading to an enhancement of maprotiline-induced apoptosis. These data suggest that ERK was a survival signal to oppose maprotiline-caused apoptotic effect in Neuro-2a cells. Thus the activation of caspase-3 by maprotiline appears to depend on the activation of JNK and the inactivation of ERK. [Ca2+]i measurement in the presence of maprotiline showed that the… Advisors/Committee Members: Shiping He (committee member), Chen-Chih Kao (chair), Chung-Ren Jan (committee member), Jing-Gung Chung (chair), Chen-Fu Shaw (chair).

Subjects/Keywords: human osteosarcoma cells; paroxetine; maprotiline; Ca2+; MAPKs; caspase-3; apoptosis; murine neuroblastoma cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chou, C. (2008). Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559

Chicago Manual of Style (16^th Edition):

Chou, Chiang-Ting. “Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline.” 2008. Doctoral Dissertation, NSYSU. Accessed April 22, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559.

MLA Handbook (7^th Edition):

Chou, Chiang-Ting. “Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline.” 2008. Web. 22 Apr 2021.

Vancouver:

Chou C. Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline. [Internet] [Doctoral dissertation]. NSYSU; 2008. [cited 2021 Apr 22]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559.

Council of Science Editors:

Chou C. Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline. [Doctoral Dissertation]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559

NSYSU

26. Zeng, Jun-Yan. New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line.

Degree: Master, Biological Sciences, 2015, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758

► Antimitotic drug, combretastatin A-4 (CA-4) is a natural plant extract exhibits powerful anticancer and anti-angiogenesis activity by targeting microtubule formation. CA-4 enediyne derivatives, such as… (more)

Subjects/Keywords: Combretastatin A-4; LO-OMe; LO-NH2; PARP; Caspase-3; Apoptosis; Autophagy; p62; LC3

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zeng, J. (2015). New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zeng, Jun-Yan. “New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line.” 2015. Thesis, NSYSU. Accessed April 22, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zeng, Jun-Yan. “New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line.” 2015. Web. 22 Apr 2021.

Vancouver:

Zeng J. New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Apr 22]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zeng J. New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

27. [No author]. Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-07082009-171902/

► Endocrine disrupting chemicals (EDCs) such as DDT have the ability to disrupt hormonally controlled processes, such as spermatogenesis, which is the maturation of germ cells… (more)

▼ Endocrine disrupting chemicals (EDCs) such as DDT have the ability to disrupt hormonally controlled processes, such as spermatogenesis, which is the maturation of germ cells into spermatozoa. During normal spermatogenesis, germ cell apoptosis can occur, but the degree of apoptosis within the testis could possibly be affected by exposure to EDCs. In 2004, a pilot study on the reproductive health of two freshwater fish species, Oreochromis mossambicus and Clarias gariepinus, from three impoundments in the Luvuvhu River, found concerning levels of DDT and its metabolites in both species from the Nandoni Dam, and in O. mossambicus from the Xikundu Weir. This was not surprising as a large part of the Luvuvhu River catchment is located within an area where ongoing DDT-spraying occurs for vector control purposes. Hence, in 2006, a larger WRC-funded project began to further investigate the findings from the pilot study. A subsidiary study, spanning two seasons, was initiated to investigate testicular apoptosis in fish from the polluted systems, the Nandoni Dam (ND) and the Xikundu Weir (XW), as well as a reference site, the Albasini Dam (AD), utilizing caspase-3 and TUNEL immunoexpression as apoptotic markers. In addition, three fixatives, Bouin’s Fluid (BF), Neutrally Buffered Formalin (NBF) and Paraformaldehyde (PFA), were used to determine which would be the optimal fixative for both histological and immunohistochemical assessments. Sampling occurred during season 1, the low-flow season (October 2007), during DDT spraying of the surrounding area, and season 2, the high–flow season (February 2008), two months after the DDT-spraying was completed. The testes of O. mossambicus (n = 19 season 1, n = 25 season 2) and C. gariepinus (n = 19 season 1, n = 20 season 2) were fixed in the above-mentioned fixatives, embedded in paraffin wax, prepared for immunohistochemistry, and exposed to caspase-3 antibodies and TUNEL antibodies individually. The results indicated that the residues of p,p´-DDT - DDD and - DDE were found in the fat samples of both O. mossambicus and C. gariepinus, in AD, ND and XW. Testicular apoptotic assessment using the caspase-3 assay clearly labeled spermatocytes in the process of cellular death in both seasons, in all three fixatives. When comparing the two assays, a significant difference is found between the caspase-3 and TUNEL positive cells. The results further show that, when comparing the three sampling sites, the highest amount of positive cells are found at the XW. The decrease observed in season two, in both the caspase-3 and TUNEL assay may possibly be linked to the stage of spermatogenesis, coinciding with hormonal changes associated with the different sampling seasons (i.e. breeding and non-breeding seasons). The levels of DDT found in the fat tissue, could not be correlated to an up-regulation in apoptotic cells. The results The results indicated that the choice of fixative, could affect the identification of the amount of positive cells. The utility of the caspase-3 and TUNEL assays, in… Advisors/Committee Members: Dr J C Van Dyk (advisor), Prof M S Bornman (advisor).

Subjects/Keywords: Apoptosis; Caspase-3 immunohistochemistry; Tunel; Oreochromis mossambicus; Clarias gariepinus; Spermatogenesis; Ddt; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2009). Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07082009-171902/

Chicago Manual of Style (16^th Edition):

author], [No. “Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays .” 2009. Masters Thesis, University of Pretoria. Accessed April 22, 2021. http://upetd.up.ac.za/thesis/available/etd-07082009-171902/.

MLA Handbook (7^th Edition):

author], [No. “Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays .” 2009. Web. 22 Apr 2021.

Vancouver:

author] [. Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays . [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Apr 22]. Available from: http://upetd.up.ac.za/thesis/available/etd-07082009-171902/.

Council of Science Editors:

author] [. Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays . [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-07082009-171902/

Universidade de Brasília

28. Sílvia Taveira Elias. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.

Degree: 2009, Universidade de Brasília

URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575

►

O câncer é um problema mundial de saúde pública. Nas últimas décadas a incidência de câncer de cabeça e pescoço vem aumentando, sendo os países… (more)

▼

O câncer é um problema mundial de saúde pública. Nas últimas décadas a incidência de câncer de cabeça e pescoço vem aumentando, sendo os países desenvolvidos os mais afetados. As terapias convencionais para este tipo de câncer, cirurgia e radioterapia, são agressivas e não aumentam de maneira considerável a qualidade e expectativa de vida dos pacientes. Nesse cenário, a descoberta de novas terapias é necessária. Esse trabalho tem como objetivo investigar o efeito citotóxico do extrato bruto de tabaco (EBT) e cinco frações provenientes dele (metanólica, neutra, carbônica, ácida e básica) em células de carcinoma oral humano. A exposição das células de câncer oral ao EBT induz a morte celular e diminui a viabilidade celular de maneira dose-dependente. Das frações testadas, apenas a neutra foi capaz de induzir uma significante morte celular (acima de 50%) após 48 horas de tratamento. Ademais, fragmentação do DNA e ativação da caspase-3 indicam que a morte celular induzida por EBT e pela fração neutra tem natureza apoptótica. Os resultados mostram que apesar do tabaco ser um carcinógeno conhecido, possuir inúmeros componentes indutores de tumor, ele também pode conter componentes capazes de induzir morte em células de câncer. Pelo fato da fração neutra ser capaz de também induzir morte celular, pode-se inferir que o componente apoptótico é apolar. Novos estudos são necessários para avaliar a estrutura química envolvida neste processo.

Cancer is a public health problem worldwide. Incidences of head and neck cancer are increasing in the last decades, and the developed countries are the most affected. Current therapeutic options for this type of cancer are aggressive, including surgery and radiotherapy. In addition, they have not yet translated into an improvement of life quality or expectancy to patients. In this scenario, new therapeuticals are urgently needed and actively sought after. The goal of this study was to investigate the cytotoxic effects of tobacco crude extract (TCE) and 5 fractions thereof (methanolic, neutral, carbonic, acid and basic) on a human oral squamous cell carcinoma. Exposure of oral cancer cells to TCE induced cell death and decreased cell viability in a dose-dependent manner. Out of the tested fractions, only the neutral one was able to induce significant cell death (over 50%) over the period of 48h. In addition, DNA laddering and caspase-3 activation indicate that the cell death processes induced by TCE and neutral fraction were apoptotic in nature. Our results indicate that although tobacco is a known carcinogen, possessing many tumorinitiating compounds, it could also contain compounds that are useful to induce apoptosis in cancer cells. Because the neutral fraction was also able to induce apoptosis, it is postulated that this putative compound is non-polar, although further investigation is needed to uncover its true nature and chemical structure.

Advisors/Committee Members: Eliete Neves da Silva Guerra, Marie Togashi, Andrea Barreto Motoyama, Fabio Pittella Silva.

Subjects/Keywords: apoptose; extrato bruto de tabaco; caspase-3; CIENCIAS DA SAUDE; câncer de boca

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Elias, S. T. (2009). Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elias, Sílvia Taveira. “Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.” 2009. Thesis, Universidade de Brasília. Accessed April 22, 2021. http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elias, Sílvia Taveira. “Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.” 2009. Web. 22 Apr 2021.

Vancouver:

Elias ST. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. [Internet] [Thesis]. Universidade de Brasília; 2009. [cited 2021 Apr 22]. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elias ST. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. [Thesis]. Universidade de Brasília; 2009. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. M.V. Traversa. NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB.

Degree: 2011, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/151771

► Hair exerts a range of functions including thermoregulation, physical protection, sensory activity. Mature and actively growing hair follicles become anchored in the subcutis, and regenerate… (more)

Subjects/Keywords: HFDP cells; androgenetic alopecia; apoptosis; caspase-3; non synthetic molecules; Settore BIO/14 - Farmacologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Traversa, M. (2011). NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/151771

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Traversa, M.V.. “NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB.” 2011. Thesis, Università degli Studi di Milano. Accessed April 22, 2021. http://hdl.handle.net/2434/151771.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Traversa, M.V.. “NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB.” 2011. Web. 22 Apr 2021.

Vancouver:

Traversa M. NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB. [Internet] [Thesis]. Università degli Studi di Milano; 2011. [cited 2021 Apr 22]. Available from: http://hdl.handle.net/2434/151771.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Traversa M. NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB. [Thesis]. Università degli Studi di Milano; 2011. Available from: http://hdl.handle.net/2434/151771

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Wayne State University

30. Feng, Ke. An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field.

Degree: PhD, Biomedical Engineering, 2017, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/1700

► Blast-induced traumatic brain injury (bTBI) is a signature wound of modern warfare. The current incomplete understanding of its injury mechanism impedes the development of… (more)

▼ Blast-induced traumatic brain injury (bTBI) is a signature wound of modern warfare. The current incomplete understanding of its injury mechanism impedes the development of strategies for effective protection of bTBI. Despite a considerable amount of experimental animal studies focused on the evaluation of brain neurotrauma caused by blast exposure, there is very limited knowledge on the biomechanical responses of the gyrenecephalic brain subjected to primary free-field blast waves imposed in vivo, and the correlation analysis between the biomechanical responses and its injury outcomes. Such information is crucial to the development of injury criteria of bTBI. This study aims to evaluate the external and internal mechanical responses of the brain against different levels of blast loading with Yucatan swine in free field, and to conduct correlational studies with brain tissue damage. To better understand primary bTBI, we have implemented an open field experimental model to apply controlled shock waves on swine head. The applied pressure levels of shock waves were predicted by finite element modeling and verified with calibrated testing. Biomechanical responses of primary blasts such as intracranial pressure (ICP), head kinetics, strain rate of skull, were measured in vivo during the blasts. A positive correlation between incident overpressure (IOP) and its corresponding biomechanical responses of the brain was observed. A parallel group of non-instrumented animals were used to collect injury data 72 hours post experiment. Cellular responses governed by primary blasts, such as neuronal degeneration and apoptosis were studied via immunohistochemistry. Representative fluorescent-stained images were examined under microscope. A positive correlation was found between the amount of degenerative neurons and the blast level. Significant elevation of apoptosis was found in the high-level blast. Comparisons between brains with varies ICP readings demonstrate differences of the numbers of neuronal degeneration and apoptosis within the imaged volume. Additionally, comparisons between sections at different locations of the head did not show spatial changes for cellular responses. These metrics provide a pathway for direct connection between the cellular damage and the measured biomechanical responses of the brain within the same experimental model, and could be critical in understanding the mechanisms of bTBI. This experimental data can be used to validate computer models of bTBI. Advisors/Committee Members: John Cavanaugh.

Subjects/Keywords: Blast; Caspase-3; FJ-C; ICP; swine; TBI; Biomechanics; Nanoscience and Nanotechnology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Feng, K. (2017). An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/1700

Chicago Manual of Style (16^th Edition):

Feng, Ke. “An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field.” 2017. Doctoral Dissertation, Wayne State University. Accessed April 22, 2021. https://digitalcommons.wayne.edu/oa_dissertations/1700.

MLA Handbook (7^th Edition):

Feng, Ke. “An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field.” 2017. Web. 22 Apr 2021.

Vancouver:

Feng K. An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field. [Internet] [Doctoral dissertation]. Wayne State University; 2017. [cited 2021 Apr 22]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1700.

Council of Science Editors:

Feng K. An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field. [Doctoral Dissertation]. Wayne State University; 2017. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1700

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