subject:(Caspase 3)

University of Edinburgh

1. Mackay, Martha. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.

Degree: PhD, 2014, University of Edinburgh

► The design and development of fluorescent reporters focussed on highly sensitive, specific, and selective imaging of cancer targets is described. These novel optical molecular probes… (more)

Subjects/Keywords: 616.99; Caspase-3; molecular detection

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APA (6^th Edition):

Mackay, M. (2014). The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/17972

Chicago Manual of Style (16^th Edition):

Mackay, Martha. “The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed April 07, 2020. http://hdl.handle.net/1842/17972.

MLA Handbook (7^th Edition):

Mackay, Martha. “The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.” 2014. Web. 07 Apr 2020.

Vancouver:

Mackay M. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/1842/17972.

Council of Science Editors:

Mackay M. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/17972

Univerzitet u Beogradu

2. Dožić, Branko S., 1968-. Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda.

Degree: Medicinski fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get

►

Medicina / Medicine

Adenoidni cistični karcinom (ACC), je jedan od najčešćih malignih tumora pljuvačnih žlezda. Učestvuje sa oko 10-15% kod salivarnih neoplazmi. Karakteriše ga spor… (more)

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Medicina / Medicine

Adenoidni cistični karcinom (ACC), je jedan od najčešćih malignih tumora pljuvačnih žlezda. Učestvuje sa oko 10-15% kod salivarnih neoplazmi. Karakteriše ga spor rast, visoka incidenca ka invaziji nerva, retko regionalno metastaziranje, česti lokalni recidivi i spor razvoj progresivnih i relativno indolentnih udaljenih metastaza. Najznačajniji faktori za prognozu ovog tumora su klinički stadijum i histološki izgled tumora, prisustvo ili odsustvo tumorskog tkiva na linijama resekcije, invazija nerva. Apoptoza je regulatorni mehanizam tkivne homeostaze. Poremećaj regulacije apoptoze je relativno česta karakteristika maligne ćelije. U zavisnosti od stimulansa, putevi aktivacije apoptoze mogu biti: receptorski (spoljašnji) i mitohondrijski (unutrašnji) put. U unutrašnjem putu apoptoze značajnu ulogu igraju Apaf-1, inicijatorne i efektorne kaspaze. Apaf-1 je multidomenski protein, za koji se vezuje citohrom c i prokaspaza 9 što dovodi do formiranja apoptozoma. Ovaj kompleks proteolitički aktivira inicijatornu kaspazu-9. U završnoj fazi apoptoze, aktivirana kaspaza 9 dovodi do aktivacije efektorne kaspaze 3, čijim ulaskom u jedro započinje degradaciona faza apoptoze. Cilj U ovoj studiji cilj je bio ispitati imunohistohemijsku ekspresiju Apaf-1, kaspaze 9 i 3 u ACC pljuvačnih žlezda, i rezultate korelirati sa kliničko-patološkim parametrima kako bi se utvrdilo koji je od njih nezavistan prognostički parametar. Materijal i metode Istraživanje je koncipirano kao studija preseka, koja obuhvata 50 pacijenata sa ACC pljuvačnih žlezda, muškog i ženskog pola, prosečne starosti 58 godina. U ovoj studiji koristili smo tehniku tkivnog mikroniza (TMA kalupi). Preseci sa TMA kalupa, debljine 5μm, bojeni su streptavidin-biotin imunohistohemijskom tehnikom pomoću primarnih antitela specifičnih za: Apaf-1, kaspazu 9 i kaspazu 3. Obojeni tkivni preseci su analizirani svetlosnim mikroskopom (Olympus tip BH-2). Na osnovu prikupljenih podataka formirana je baza podataka u –SPSS 18.0 koja je korišćena za dalju statističku obradu. Statistička analiza podataka obuhvatala je metode deskriptivne i analitičke (inferencijalne) statistike...

Advisors/Committee Members: Boričić, Ivan, 1956-.

Subjects/Keywords: adenoid cystic carcinoma; apoptosis; Apaf-1; caspase 9; caspase 3.

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APA (6^th Edition):

Dožić, Branko S., 1. (2016). Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dožić, Branko S., 1968-. “Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda.” 2016. Thesis, Univerzitet u Beogradu. Accessed April 07, 2020. https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dožić, Branko S., 1968-. “Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda.” 2016. Web. 07 Apr 2020.

Vancouver:

Dožić, Branko S. 1. Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2020 Apr 07]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dožić, Branko S. 1. Imunohistohemijska analiza i prognostički značaj markera unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu pljuvačnih žlezda. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

North Carolina State University

3. Walters, Jad Anthony. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.

Degree: PhD, Biochemistry, 2009, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/3363

► Effector procaspase-3 plays a vital role in carrying out the final steps of programmed cell death, leading to the destruction of the cell. Because dimerization… (more)

Subjects/Keywords: Activation; Inhibition; Dimer Interface; (Pro)caspase-3

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APA (6^th Edition):

Walters, J. A. (2009). Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3363

Chicago Manual of Style (16^th Edition):

Walters, Jad Anthony. “Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.” 2009. Doctoral Dissertation, North Carolina State University. Accessed April 07, 2020. http://www.lib.ncsu.edu/resolver/1840.16/3363.

MLA Handbook (7^th Edition):

Walters, Jad Anthony. “Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.” 2009. Web. 07 Apr 2020.

Vancouver:

Walters JA. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. [Internet] [Doctoral dissertation]. North Carolina State University; 2009. [cited 2020 Apr 07]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3363.

Council of Science Editors:

Walters JA. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. [Doctoral Dissertation]. North Carolina State University; 2009. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3363

University of Otago

4. Douglas, Hollian Raphaelle. XIAP, a potential regulator of follicular atresia in the sheep ovary .

Degree: 2011, University of Otago

URL: http://hdl.handle.net/10523/1635

► X-linked inhibitor of apoptosis (XIAP) is a strong inhibitor of initiator (-9) and executer (-3 and -7) caspase activity (Eckelman and Salvesen, 2006). In female… (more)

▼ X-linked inhibitor of apoptosis (XIAP) is a strong inhibitor of initiator (-9) and executer (-3 and -7) caspase activity (Eckelman and Salvesen, 2006). In female reproductive research, XIAP has received minimal attention considering the ability to arrest caspases provides a potential mechanism for regulation of follicular atresia. This study aimed to test the hypotheses that XIAP is indicative of follicular health and regulates follicular atresia and to investigate the novel idea that prolactin (PRL) stimulates XIAP expression in the sheep ovary. Estrous cycles of adult Romney ewes (N=31) were synchronized with Estrumate®. Tissue and blood samples were subsequently collected on either days 14, 15 and 16 or at twelve hour intervals during the follicular phase. Initially analysis involved developing a plasma PRL profile by radioimmunoassay. The presence and localization patterns of XIAP and prolactin receptor (PRLR) isoforms protein and/or mRNA were determined by RT-PCR, in situ hybridization histochemistry and immunohistochemistry. This was followed by a comparative study evaluating levels of active caspase-3 immunoreactivity and XIAP mRNA (N=22) or protein (N=23) expression in adjacent sections containing the same day 14, 15 and 16 antral follicles. Triple label immunofluorescence and confocal microscopy were subsequently used to further clarify XIAP and active caspase-3 protein localization and to establish the presence of an inverse expression relationship in granulosa and thecal cells. Differing doses of Ovine FSH and PRL, as potential stimuli of XIAP upregulation, were trialed in cultured granulosa cells. Finally, a provisional attempt to develop a granulosa cell death model for determination of PRL’s ability to upregulate XIAP was undertaken. This study showed widespread XIAP expression during both luteal and follicular phases of the estrous cycle. XIAP protein was detected from the primary follicle stage onwards, whereas the mRNA was only evident in antral follicles, likely due to limited sensitivity of the in situ hybridization histochemical technique. This also proved problematic for detection of PRLR isoform mRNA, which was localized in luteal cells and three antral follicles only. In the comparative analysis, all day 14 antral follicles showed positive XIAP mRNA expression irrespective of active caspase-3 levels. Over 50% of the day 15 and 16 antral follicles scored, however, showed an inverse relationship between XIAP mRNA/protein and active caspase-3 protein levels in, as did XIAP protein in day 14 antral follicles. Interestingly, in healthy follicles XIAP expression in thecal tissue was widespread, becoming increasingly localized to interna layers as active caspase-3 immunopositive granulosa cells became prevalent, despite a lack of caspase activity in the theca itself. Confocal microscopy showed active caspase- 3 and XIAP colocalization in granulosa cells. No significant negative correlation in expression patterns was observed, possibly due to brief active caspase-3 expression at apoptosis onset… Advisors/Committee Members: Hurst, Peter (advisor).

Subjects/Keywords: Ovary; XIAP; Active caspase-3; Follicle

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APA (6^th Edition):

Douglas, H. R. (2011). XIAP, a potential regulator of follicular atresia in the sheep ovary . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1635

Chicago Manual of Style (16^th Edition):

Douglas, Hollian Raphaelle. “XIAP, a potential regulator of follicular atresia in the sheep ovary .” 2011. Doctoral Dissertation, University of Otago. Accessed April 07, 2020. http://hdl.handle.net/10523/1635.

MLA Handbook (7^th Edition):

Douglas, Hollian Raphaelle. “XIAP, a potential regulator of follicular atresia in the sheep ovary .” 2011. Web. 07 Apr 2020.

Vancouver:

Douglas HR. XIAP, a potential regulator of follicular atresia in the sheep ovary . [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/10523/1635.

Council of Science Editors:

Douglas HR. XIAP, a potential regulator of follicular atresia in the sheep ovary . [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1635

5. Coutinho, Ana Rita Sousa. Presença da Caspase-3 ativa e das proteínas de reparo de lesões do DNA em embriões suínos ativados partenogeneticamente com alta ou baixa capacidade de desenvolvimento.

Degree: PhD, Reprodução Animal, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-12122007-100619/ ;

► Apoptose é uma forma de morte celular extremamente conservada que tem papel primordial no desenvolvimento animal e na homeostasia celular, agindo como mecanismo de controle… (more)

▼ Apoptose é uma forma de morte celular extremamente conservada que tem papel primordial no desenvolvimento animal e na homeostasia celular, agindo como mecanismo de controle de qualidade com a função de remover células danificadas, em excesso ou não-funcionais. Este processo tem papel importante no desenvolvimento embrionário pré-implantacional, pois as células embrionárias estão sujeitas aos danos no DNA que podem ativar mecanismos de reparo de DNA ou induzir apoptose, que culmina com a ativação da enzima caspase-3 responsável pela clivagem de vários substratos celulares. Deste modo, o presente trabalho teve como objetivos determinar a presença da Caspase-3 ativa (+casp-3) e sua influência no desenvolvimento de embriões suínos no estágio de pré-implantação, bem como investigar a detecção de proteínas que atuam no mecanismo de lesão e reparo do DNA. Foram realizados 4 experimentos com oócitos imaturos de fêmeas pré-púberes, obtidos de ovários oriundos de abatedouro, maturados in vitro (MIV) por 44-46 horas. Oócitos em metáfase II (MII) foram ativados partenogeneticamente (AP) com ionomicina e cloreto de estrôncio e cultivados in vitro em PZM3 a 38ºC e 5% CO2 em ar e alta umidade. No primeiro experimento os embriões foram classificados em 4 grupos de acordo com o tempo de clivagem e o número de células: R4 - clivado às 24 horas e com ≥4 células às 48 horas; R2 - clivado às 24 horas e com 2 ou 3 células às 48 horas; L4 - não clivado às 24 horas e com ≥4 células às 48 horas; L2 - não clivado às 24 horas e com 2 ou 3 células às 48 horas. Os embriões foram então avaliados no D-6 do CIV para determinação do índice de desenvolvimento até o estágio de blastocisto. Os embriões do grupo R apresentaram maiores índices de blastocisto, R4 - 66,1 (174/263) e R2 - 59,6 (62/104) e maior número de núcleos por blastocisto, R4 - 40,1 e R2 - 37,8, quando comparados ao grupo L, L4 - 15,4 (6/39) e L2-16,8 (13/77); L4-18,5 e L2-18,3 (Qui-quadrado e Tukey-Kramer, respectivamente, P<0,05); entretanto, não houve diferença entre os grupos R4 e R2 e L4 e L2. Para o segundo experimento utilizou-se apenas embriões R e L, classificados às 24hs do início do CIV. Ambos os grupos (R e L) foram destinados à análise de imunocitoquímica para +casp-3 fixados nos D2, D4 e D6, sendo cada um destes dias considerado um sub-experimento e realizado em manipulações diferentes. Foi observada localização citoplasmática da +casp-3 do D2 ao D6 e nuclear a partir do D5 de cultivo. A quantificação relativa da +casp-3 nos grupos R e L de cultivo foi de 2,4 vs 1,4; 1,1 vs 1,0 e 1,1 vs 1,2 para o D2, D4 e D6, respectivamente. Para avaliar a influência da +casp-3 no desenvolvimento de embriões suínos foi realizado o terceiro experimento utilizando o inibidor de caspase z-DEVD-fmk (100uM) durante a MIV, no início (D0 ao D2) ou no final (D4 ao D6) do cultivo. Embriões produzidos sem inibidor foram usados como controle. A presença do inibidor durante a MIV e no final do cultivo não afetou os índices de blastocistos. No entanto, a presença do inibidor durante as… Advisors/Committee Members: Assumpção, Mayra Elena Ortiz D\'Avila.

Subjects/Keywords: Apoptose; Apoptosis; Ativação partenogenetica; Caspase-3; Caspase-3; Embriões; Embryos; Parthenogenetic activation; Suínos; Swine

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APA (6^th Edition):

Coutinho, A. R. S. (2007). Presença da Caspase-3 ativa e das proteínas de reparo de lesões do DNA em embriões suínos ativados partenogeneticamente com alta ou baixa capacidade de desenvolvimento. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-12122007-100619/ ;

Chicago Manual of Style (16^th Edition):

Coutinho, Ana Rita Sousa. “Presença da Caspase-3 ativa e das proteínas de reparo de lesões do DNA em embriões suínos ativados partenogeneticamente com alta ou baixa capacidade de desenvolvimento.” 2007. Doctoral Dissertation, University of São Paulo. Accessed April 07, 2020. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-12122007-100619/ ;.

MLA Handbook (7^th Edition):

Coutinho, Ana Rita Sousa. “Presença da Caspase-3 ativa e das proteínas de reparo de lesões do DNA em embriões suínos ativados partenogeneticamente com alta ou baixa capacidade de desenvolvimento.” 2007. Web. 07 Apr 2020.

Vancouver:

Coutinho ARS. Presença da Caspase-3 ativa e das proteínas de reparo de lesões do DNA em embriões suínos ativados partenogeneticamente com alta ou baixa capacidade de desenvolvimento. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2020 Apr 07]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-12122007-100619/ ;.

Council of Science Editors:

Coutinho ARS. Presença da Caspase-3 ativa e das proteínas de reparo de lesões do DNA em embriões suínos ativados partenogeneticamente com alta ou baixa capacidade de desenvolvimento. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-12122007-100619/ ;

6. Amptoulach, Sousana. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.

Degree: 2015, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/41730

► There is strong evidence that tumor growth is not only a result of uncontrolled cell proliferation but alsoof decreased apoptosis. Caspase-3 is a member of… (more)

Subjects/Keywords: Κασπάση-3; Απόπτωση; Γαστρικός καρκίνος; Caspase-3; Apoptosis; Gasric cancer

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APA (6^th Edition):

Amptoulach, S. (2015). Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/41730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amptoulach, Sousana. “Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.” 2015. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 07, 2020. http://hdl.handle.net/10442/hedi/41730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amptoulach, Sousana. “Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.” 2015. Web. 07 Apr 2020.

Vancouver:

Amptoulach S. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/10442/hedi/41730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amptoulach S. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. Available from: http://hdl.handle.net/10442/hedi/41730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. 川元, 康嗣. TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.

Degree: 博士(医学), 2016, Nagasaki University / 長崎大学

URL: http://hdl.handle.net/10069/37166

► Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp… (more)

Subjects/Keywords: Anti-TNF-α therapy; Cell death; Caspase 3; Caspase 7; Lung epithelial cells

3 more images…

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APA (6^th Edition):

川元, �. (2016). TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/37166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

川元, 康嗣. “TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed April 07, 2020. http://hdl.handle.net/10069/37166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

川元, 康嗣. “TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.” 2016. Web. 07 Apr 2020.

Vancouver:

川元 �. TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/10069/37166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

川元 �. TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/37166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McGill University

8. Shao, Wei, 1970-. Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway.

Degree: MS, Department of Biochemistry., 2007, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile100248.pdf

► Apoptosis is executed by caspase-mediated cleavage of various proteins. Elucidating the consequence of substrate cleavage provides us with insight into cell death and other biological… (more)

Subjects/Keywords: Apoptosis.; Caspase 1.; Caspase 3.; Glycolysis.

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APA (6^th Edition):

Shao, Wei, 1. (2007). Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway. (Masters Thesis). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile100248.pdf

Chicago Manual of Style (16^th Edition):

Shao, Wei, 1970-. “Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway.” 2007. Masters Thesis, McGill University. Accessed April 07, 2020. http://digitool.library.mcgill.ca/thesisfile100248.pdf.

MLA Handbook (7^th Edition):

Shao, Wei, 1970-. “Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway.” 2007. Web. 07 Apr 2020.

Vancouver:

Shao, Wei 1. Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway. [Internet] [Masters thesis]. McGill University; 2007. [cited 2020 Apr 07]. Available from: http://digitool.library.mcgill.ca/thesisfile100248.pdf.

Council of Science Editors:

Shao, Wei 1. Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway. [Masters Thesis]. McGill University; 2007. Available from: http://digitool.library.mcgill.ca/thesisfile100248.pdf

9. Terra, Adriana Cristina. Interferências do campo elétrico alternado externo em células tumorais e normais.

Degree: Mestrado, Biotecnologia, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30042010-090133/ ;

►

Os campos elétricos alternados de frequência intermediária (10 KHz a 1MHz) e baixa intensidade têm efeitos inibitórios sobre o crescimento de várias linhagens celulares de… (more)

▼

Os campos elétricos alternados de frequência intermediária (10 KHz a 1MHz) e baixa intensidade têm efeitos inibitórios sobre o crescimento de várias linhagens celulares de tumores humanos e murinos. O objetivo geral do presente trabalho é conhecer os efeitos biológicos da aplicação externa de Campos Elétricos Alternados de intensidades de 10 V e 5 V nas frequências de 200kHz, 1Mhz e 2Mhz; em culturas de células de melanoma murino (B16F10) e fibroblastos humanos normais (FN1), durante 24 horas. Foram estudados os efeitos biológicos antiproliferativos e pró-apoptóticos através das seguintes análises: Citometria de Fluxo para identificar a distribuição das populações celulares nas fases do ciclo celular, Colorimétrico MTT para avaliar a viabilidade celular e Peroxidação Lipídica para avaliar a produção de radicais livres lipídicos poliinsaturados. Os resultados mostraram que em fibroblastos humanos normais o campo elétrico alternado induziu efeito antiproliferativo, indutor de necrose e apoptose, porém em menor efeito tóxico quando comparado às células de melanoma (B16F10), principalmente na frequência de 200 kHz. Por outro lado, na frequência de 2MHz e intensidade 10V, as células de melanoma presentes no sobrenadante e aderentes expressaram diferencialmente número de células mortas por apoptose (Anexina-V) e caspase-3 fosforilada. Os diferentes efeitos produzidos entre as duas linhagens estudadas atentam para o fato de que a compreensão dos mecanismos biofísicos da regulação do reparo e da proliferação celular envolve fenômenos celulares e vias de sinalização altamente complexas.

Electric fields of alternating intermediate frequency and low intensity have inhibitory effects on the growth of various tumor cell lines. The objective of this study was to evaluate the biological effects of external application of external alternating electric field (CEAE) intensities of 10 V and 5 V in the frequency 200kHz, 1MHz and 2MHz; in cultured murine melanoma cells (B16F10) and human fibroblasts normal (FN1). We studied the antiproliferative and pro-apoptotic by flow cytometry to identify the distribution of cell populations in the phases of the cell cycle, Colorimetric MTT to evaluate cell viability and lipid peroxidation to evaluate the production of free radicals lipid acids. The results showed that in fibroblasts (FN 1) the normal CEAE induced antiproliferative effect, inducing apoptosis and necrosis, but at a lower cytotoxic potential when applied to melanoma cells (B16F10), mainly in the frequency of 200 kHz. Moreover, the frequency of 2MHz and intensity 10V, the melanoma cells in supernatant and the members expressed differentially number of dead cells by apoptosis and caspase-3 phosphorylated. The different effects between the two strains studied to undermine the fact that understanding the biophysical mechanisms of regulation of repair and cell proliferation involves cellular phenomena and signaling pathways highly specific and complex.

Advisors/Committee Members: Maria, Durvanei Augusto.

Subjects/Keywords: Alternated eletric field; Apoptose; Apoptosis; Campo elétrico alternado; Caspase-3; Caspase-3; Cell cycle; Ciclo celular; Citotoxicidade; Citotoxicity; Melanoma; Melanoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Terra, A. C. (2010). Interferências do campo elétrico alternado externo em células tumorais e normais. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30042010-090133/ ;

Chicago Manual of Style (16^th Edition):

Terra, Adriana Cristina. “Interferências do campo elétrico alternado externo em células tumorais e normais.” 2010. Masters Thesis, University of São Paulo. Accessed April 07, 2020. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30042010-090133/ ;.

MLA Handbook (7^th Edition):

Terra, Adriana Cristina. “Interferências do campo elétrico alternado externo em células tumorais e normais.” 2010. Web. 07 Apr 2020.

Vancouver:

Terra AC. Interferências do campo elétrico alternado externo em células tumorais e normais. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2020 Apr 07]. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30042010-090133/ ;.

Council of Science Editors:

Terra AC. Interferências do campo elétrico alternado externo em células tumorais e normais. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-30042010-090133/ ;

Laurentian University

10. Albalawi, Ghadah. The effect of andrographis paniculata on the growth of malignant cancer cells .

Degree: 2016, Laurentian University

URL: https://zone.biblio.laurentian.ca/handle/10219/2674

► There are a variety of plants that have been recognized and used in traditional medicine for their health benefits. Among these plants is Andrographis paniculata,… (more)

Subjects/Keywords: Andrographis paniculata; malignant cancer cells; Apoptosis; Caspase 3,; Sub-G1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albalawi, G. (2016). The effect of andrographis paniculata on the growth of malignant cancer cells . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/handle/10219/2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells .” 2016. Thesis, Laurentian University. Accessed April 07, 2020. https://zone.biblio.laurentian.ca/handle/10219/2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells .” 2016. Web. 07 Apr 2020.

Vancouver:

Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells . [Internet] [Thesis]. Laurentian University; 2016. [cited 2020 Apr 07]. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells . [Thesis]. Laurentian University; 2016. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

11. Voss, Oliver H. Regulation of Cell Fate by Caspase-3.

Degree: PhD, Molecular Genetics, 2010, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1281536983

► Monocytes and lymphocytes are crucial components of the innate and adaptive immune system. These cells are generated daily in the bone marrow and, when unchallenged,… (more)

▼ Monocytes and lymphocytes are crucial components of the innate and adaptive immune system. These cells are generated daily in the bone marrow and, when unchallenged, undergo spontaneous apoptosis while requiring the activation of caspase-3. Differentiation factors, pathogens or antigens inhibit caspase-3 thereby promoting prolonged cellular life span. Upon clearance of the inflammation, activated monocytes or lymphocytes re-enter the apoptotic program allowing the restoration of cell numbers. However, dysregulation of apoptosis has been linked to numerous inflammatory diseases and hematologic malignancies including rheumatoid arthritis, atherosclerosis, monocytic leukemia, and T-cell large granular lymphocyte (T-LGL) leukemia.In this study, we found, using primary human monocytes and monocytic THP-1 cells, that caspase-3 associates with PKCd in vitro and in vivo. We demonstrated that caspase-3 is a phosphoprotein using in vivo labeling experiments and in vitro kinase assays. We mapped the domains of these molecules involved in the interaction using purified proteins and transient over-expression expriments both in vitro and in vivo. Moreover, we identified the PKCd-dependent phosphorylation sites on caspase-3 using mass spectrometry. In addition, we found that direct binding of the caspase-3 prodomain with Hsp27 in non-apoptotic primary human monocytes regulates monocyte survival and differentiation into primary macrophages. We found that over-expression of Hsp27 promoted survival, while silencing of Hsp27 induced caspase-3-mediated cell death. We showed a differential localization of caspase-3 and its regulator Hsp27 during the life span of monocytes and during monocyte to macrophage differentiation. These results support a model in which Hsp27 acts as an anti-apoptotic regulator of caspase-3. Moreover, we showed that Hsp27 is a key regulator of lymphocyte cell fate. Unlike other Hsps and pro- and anti-apoptotic BCL-2-family members, we found that Hsp27 expression was tightly associated with cellular life span of CD8+CD57+/-. We demonstrated that Hsp27, in a concentration-dependent manner, prolonged survival of cytotoxic CD8+CD57+, whereas silencing promoted the activity and activation of caspase-3 initiated apoptosis in CD8+CD57-. Collectively, these results provide preliminary evidence on how Hsp27 and PKCd act as anti- and pro-apoptotic regulators, respectively, in caspase-3 mediated apoptosis. Furthermore, they emphasize the potential of Hsp27 as a useful marker of cellular life span in cells of the innate and acquired immune system and highlight Hsp27 a possible therapeutic target to induce cell death in cancer cells. Advisors/Committee Members: Doseff, Andrea (Advisor).

Subjects/Keywords: Molecular Biology; Caspase-3, Hsp27, PKC&948; , Apoptosis, Monocytes, Macrophages, Lymphocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Voss, O. H. (2010). Regulation of Cell Fate by Caspase-3. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1281536983

Chicago Manual of Style (16^th Edition):

Voss, Oliver H. “Regulation of Cell Fate by Caspase-3.” 2010. Doctoral Dissertation, The Ohio State University. Accessed April 07, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1281536983.

MLA Handbook (7^th Edition):

Voss, Oliver H. “Regulation of Cell Fate by Caspase-3.” 2010. Web. 07 Apr 2020.

Vancouver:

Voss OH. Regulation of Cell Fate by Caspase-3. [Internet] [Doctoral dissertation]. The Ohio State University; 2010. [cited 2020 Apr 07]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1281536983.

Council of Science Editors:

Voss OH. Regulation of Cell Fate by Caspase-3. [Doctoral Dissertation]. The Ohio State University; 2010. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1281536983

NSYSU

12. Liang, Wei-Zhe. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.

Degree: PhD, Biological Sciences, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

► The effect of the natural essential oil carvacrol or the anesthetic propofol on cell viability, cell cycle distribution, reactive oxygen species (ROS), intracellular free Ca2+… (more)

▼ The effect of the natural essential oil carvacrol or the anesthetic propofol on cell viability, cell cycle distribution, reactive oxygen species (ROS), intracellular free Ca2+ concentrations ([Ca2+]i) and caspase-3-associated apoptosis in human normal cells or non-normal cells is unclear. Human gingival fibroblasts (HGF), human oral cancer cell line (OC2) and human glioblastoma cell line (DRTBG-05MG, HGB) were used in this study. Cell viability was measured by detecting reagent water soluble tetrazolium salt-1 (WST-1). Apoptosis was detected by Annexin V/propidium iodide (PI) staining, cell cycle distribution was detected by PI staining, and ROS was detected by membrane-permeable probe dichlorofluorescein diacetate (DCFH-DA) or hydroethidine (HE) staining. Apoptosis, cell cycle distribution and ROS were analyzed by flow cytometry. The Ca2+-sensitive fluorescent dye fura-2 was applied to measure [Ca2+]i. Caspase-3 expression was detected by western blotting. Carvacrol at 200-800 Î¼M decreased the cell viability of OC2 or HGB cells in a concentration-dependent manner and 1,000 Î¼M carvacrol almost killed all OC2 or HGB cells, but in HGF cells, 200-800 Î¼M carvacrol did not significantly kill cells and 1,000 Î¼M carvacrol decreased only about 63% of cell viability. Similarly, propofol at concentrations between 300 and 600 Î¼M decreased the cell viability of OC2 or HGB cells in a concentration-dependent manner and 700 Î¼M propofol almost killed all OC2 or HGB cells, but in HGF cells, 300-600 Î¼M propofol did not significantly kill cells and 700 Î¼M propofol decreased about 62% of cell viability. In OC2 or HGB cells, carvacrol (200 Î¼M, 400 Î¼M or 600 Î¼M) or propofol (300 Î¼M, 400 Î¼M or 500 Î¼M) induced apoptosis, increased ROS production, evoked cell cycle arrest and activated caspase-3. The caspase-3 inhibitor (DEVD-CHO) partially decreased the apoptotic effect induced by carvacrol or propofol. On the other hand, in OC2 or HGB cells, carvacrol at concentrations between 400 Î¼M and 1,000 Î¼M induced a [Ca2+]i rise in a concentration-dependent manner and the signal was reduced by removal of extracellular Ca2+. In HGF cells, carvacrol at 1000 Î¼M did not induce immediate [Ca2+]i rises in Ca2+-containing or Ca2+-free medium. Similarly, propofol at concentrations between 400 Î¼M and 1,000 Î¼M induced a [Ca2+]i rise in a concentration-dependent manner in OC2 or HGB cells, but not in HGF cells. This cytotoxic effect was not reversed in carvacrol-treated groups, but was partially reversed in propofol-treated groups when cytosolic Ca2+ was chelated with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA-AM) in OC2 or HGB cells. The apoptotic effect of propofol was also partially decreased by BAPTA-AM treatment in OC2 and HGB cells. In OC2 and HGB cells, carvacrol- or propofol-induced Ca2+ signal was not altered by L-type voltage-gated Ca2+ channel blocker nifedipine, store-operated Ca2+ channel blocker econazole or SK&F96365) and protein kinase C (PKC) activator phorbol myristate acetate (PMA), but… Advisors/Committee Members: Nai-Wen Tsai (chair), Chung-Ren Jan (chair), Ko-Long Lin (chair), Cheng-Hsien Lu (committee member), Yao-Dung Hsieh (chair).

Subjects/Keywords: Apoptosis; cell cycle; ROS; Ca2+; Caspase-3; Propofol; Carvacrol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liang, W. (2012). Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

Chicago Manual of Style (16^th Edition):

Liang, Wei-Zhe. “Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.” 2012. Doctoral Dissertation, NSYSU. Accessed April 07, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617.

MLA Handbook (7^th Edition):

Liang, Wei-Zhe. “Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.” 2012. Web. 07 Apr 2020.

Vancouver:

Liang W. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. [Internet] [Doctoral dissertation]. NSYSU; 2012. [cited 2020 Apr 07]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617.

Council of Science Editors:

Liang W. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. [Doctoral Dissertation]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

Louisiana State University

13. Gill, Amy F. The role of bone marrow in SIV pathogenesis using the Rhesus macaque model.

Degree: PhD, Veterinary Pathology and Pathobiology, 2010, Louisiana State University

URL: etd-06072010-090000 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2490

► CD4+ memory T cells are depleted in mucosal tissues post human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection without restoration to pre-infection levels… (more)

▼ CD4+ memory T cells are depleted in mucosal tissues post human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection without restoration to pre-infection levels during progressive course of disease. Bone marrow (BM) as a hematopoietic organ has been investigated for hematologic and morphologic changes during HIV infection. However, BM as a primary lymphoid tissue during HIV infection has been poorly characterized. We proposed BM was also a site of CD4+ T cell depletion driven by increased apoptosis during progressive HIV disease. We chose to investigate bone marrow changes using the premier non-human primate Rhesus macaque SIV model for the study HIV infection. We observed hematologic abnormalities of anemia, thrombocytopenia, neutropenia and eosinophilia during SIV infection mimicked HIV infection as did morphologic increased BM cellularity, loss of iron marrow storage, and increased marrow fibrosis as infection advanced to AIDS. The increased BM cellularity was characterized by increased erythroid, myeloid including dendritic cells, and lymphoid lineages in the later stages of infection. In fact, numbers of BM CD3+ T lymphocytes increased in absolute numbers and proliferation percentage during progressive SIV infection mainly composed of CD8+ T cells and fewer CD4+ T cells. Naïve and memory CD8+ T cells and CD4+ T cells were maintained in BM during SIV infection. Low numbers of SIV infected BM cells were observed including CD3+ T cells, macrophages, and other hematopoietic cells with detection of both viral RNA and DNA by polymerase chain reaction. However, less than 0.2% of CD3+ BM T cells were apoptotic determined by activated caspase 3 though overall BM cells tended to have increased rates of apoptosis in later stages of SIV. Our data revealed BM T cells were not depleted but maintained to increased during SIV disease without an increase in apoptosis. Future studies into mechanisms of bone marrow lymphocyte maintenance may reveal homeostatic mechanisms potentially disrupted in mucosal tissues during HIV and SIV infection.

Subjects/Keywords: flow cytometry; immunohistochemistry; in situ hybridization; TUNEL; activated caspase 3; cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gill, A. F. (2010). The role of bone marrow in SIV pathogenesis using the Rhesus macaque model. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-06072010-090000 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2490

Chicago Manual of Style (16^th Edition):

Gill, Amy F. “The role of bone marrow in SIV pathogenesis using the Rhesus macaque model.” 2010. Doctoral Dissertation, Louisiana State University. Accessed April 07, 2020. etd-06072010-090000 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2490.

MLA Handbook (7^th Edition):

Gill, Amy F. “The role of bone marrow in SIV pathogenesis using the Rhesus macaque model.” 2010. Web. 07 Apr 2020.

Vancouver:

Gill AF. The role of bone marrow in SIV pathogenesis using the Rhesus macaque model. [Internet] [Doctoral dissertation]. Louisiana State University; 2010. [cited 2020 Apr 07]. Available from: etd-06072010-090000 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2490.

Council of Science Editors:

Gill AF. The role of bone marrow in SIV pathogenesis using the Rhesus macaque model. [Doctoral Dissertation]. Louisiana State University; 2010. Available from: etd-06072010-090000 ; https://digitalcommons.lsu.edu/gradschool_dissertations/2490

University of Miami

14. Gillespie, Megan Marie. Improving Sensing Through the Design of Recombinant Proteins.

Degree: PhD, Chemistry (Arts and Sciences), 2015, University of Miami

URL: https://scholarlyrepository.miami.edu/oa_dissertations/1460

► Genetic engineering has provided us with the ability to advance medicine and technology by creating proteins that can be used for various applications. Two such… (more)

Subjects/Keywords: protein engineering; apoptosis; cornea; caspase-3; aequorin; glucose oxidase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gillespie, M. M. (2015). Improving Sensing Through the Design of Recombinant Proteins. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/1460

Chicago Manual of Style (16^th Edition):

Gillespie, Megan Marie. “Improving Sensing Through the Design of Recombinant Proteins.” 2015. Doctoral Dissertation, University of Miami. Accessed April 07, 2020. https://scholarlyrepository.miami.edu/oa_dissertations/1460.

MLA Handbook (7^th Edition):

Gillespie, Megan Marie. “Improving Sensing Through the Design of Recombinant Proteins.” 2015. Web. 07 Apr 2020.

Vancouver:

Gillespie MM. Improving Sensing Through the Design of Recombinant Proteins. [Internet] [Doctoral dissertation]. University of Miami; 2015. [cited 2020 Apr 07]. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1460.

Council of Science Editors:

Gillespie MM. Improving Sensing Through the Design of Recombinant Proteins. [Doctoral Dissertation]. University of Miami; 2015. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1460

University of Toronto

15. Latif, Maya. Regulation of Cisplatin-induced Programmed Cell Death In Vivo.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/82403

►

Cisplatin [ cis-PtCl2 NH3)2 ] is a widely utilized chemotherapeutic agent. Its proposed mechanism of action involves DNA-platinum adduct formation that triggers DNA damage responses,… (more)

Subjects/Keywords: Apoptosis; Caspase-3; Cisplatin; DNA damage; Necroptosis; PCD; 0307

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Latif, M. (2016). Regulation of Cisplatin-induced Programmed Cell Death In Vivo. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/82403

Chicago Manual of Style (16^th Edition):

Latif, Maya. “Regulation of Cisplatin-induced Programmed Cell Death In Vivo.” 2016. Masters Thesis, University of Toronto. Accessed April 07, 2020. http://hdl.handle.net/1807/82403.

MLA Handbook (7^th Edition):

Latif, Maya. “Regulation of Cisplatin-induced Programmed Cell Death In Vivo.” 2016. Web. 07 Apr 2020.

Vancouver:

Latif M. Regulation of Cisplatin-induced Programmed Cell Death In Vivo. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/1807/82403.

Council of Science Editors:

Latif M. Regulation of Cisplatin-induced Programmed Cell Death In Vivo. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/82403

Utah State University

16. Harding, Charles P. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.

Degree: MS, Biological and Irrigation Engineering, 2019, Utah State University

URL: https://digitalcommons.usu.edu/etd/7436

► Muscle loss from lack of activity is a serious issue for immobilized patients on Earth and in human spaceflight, where the low gravity environment… (more)

Subjects/Keywords: MuRF1; MAFbx; Caspase 3; C2C12; RCCS; Biological Engineering

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APA (6^th Edition):

Harding, C. P. (2019). In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/7436

Chicago Manual of Style (16^th Edition):

Harding, Charles P. “In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.” 2019. Masters Thesis, Utah State University. Accessed April 07, 2020. https://digitalcommons.usu.edu/etd/7436.

MLA Handbook (7^th Edition):

Harding, Charles P. “In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.” 2019. Web. 07 Apr 2020.

Vancouver:

Harding CP. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. [Internet] [Masters thesis]. Utah State University; 2019. [cited 2020 Apr 07]. Available from: https://digitalcommons.usu.edu/etd/7436.

Council of Science Editors:

Harding CP. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. [Masters Thesis]. Utah State University; 2019. Available from: https://digitalcommons.usu.edu/etd/7436

Laurentian University

17. Albalawi, Ghadah. The effect of andrographis paniculata on the growth of malignant cancer cells .

Degree: 2016, Laurentian University

URL: https://zone.biblio.laurentian.ca/handle/10219/2674

► There are a variety of plants that have been recognized and used in traditional medicine for their health benefits. Among these plants is Andrographis paniculata,… (more)

Subjects/Keywords: Andrographis paniculata; malignant cancer cells; Apoptosis; Caspase 3,; Sub-G1

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albalawi, G. (2016). The effect of andrographis paniculata on the growth of malignant cancer cells . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/handle/10219/2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells .” 2016. Thesis, Laurentian University. Accessed April 07, 2020. https://zone.biblio.laurentian.ca/handle/10219/2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells .” 2016. Web. 07 Apr 2020.

Vancouver:

Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells . [Internet] [Thesis]. Laurentian University; 2016. [cited 2020 Apr 07]. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells . [Thesis]. Laurentian University; 2016. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Ζυγούρης, Παναγιώτης. Μελέτη της έκφρασης της τελομεράσης στα μελανώματα σε συσχετισμό με κλινικές παραμέτρους και δείκτες απόπτωσης.

Degree: 2009, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/24256

►

BACKGROUND: Deregulation of apoptotic pathways in malignant cutaneous melanoma seems to be correlated with chemo resistance and poor prognosis. Furthermore, telomerase (especially h TERT) expression… (more)

▼

BACKGROUND: Deregulation of apoptotic pathways in malignant cutaneous melanoma seems to be correlated with chemo resistance and poor prognosis. Furthermore, telomerase (especially h TERT) expression represents a potential target for vaccination regarding some types of malignancies. MATERIALS AND METHODS: Using tissue microarray technology, twenty-five (n=25) paraffin embedded tissue samples of histologically confirmed malignant melanomas were cored at a diameter of 2mm and re-embedded into one final recipient block. Immunohistochemistry was performed by the use of anti-bcl2, anti-caspase3, anti-caspase8 and anti-h TERT antibodies. Protein expression levels were evaluated using an image analysis system. SPSS (chi square test and interrater kappa) was performed for statistical analysis. RESULTS: High level protein overexpression was observed in 1/24 (4.1%), 1/24 (4.1%), 2/24 (8.2%), and 4/24 (17.4%) cases regarding h TERT, Caspase3, Caspase8 και bcl2. Moderate level was observed in 7/24 (29.1%), 8/24 (32.2%), 5/24 (20.2%), and 8/24(32.2%) cases, respectively, whereas reduced or absent expression demonstrated 16/24 (65%), 15/24 (60.2%), 17/24 (68.5%), and 12/24 (50%) cases, respectively. Statistical significance was assessed correlating age to Caspase3 (p=0.05), Breslow to telomerase (p=0.013), Breslow to bcl2 (p=0.053), Clark to telomerase (p=0.008), Clark to bcl2 (p=0.022), and finally ulceration to telomerase (p=0.007). Regarding the intercorrelations between hTERT, caspase-3, caspase-8 and bcl-2 expression, only two arose at a statistically significant level; hTERT expression was positively associated with bcl-2 expression (Spearman’s rho = 0.508, p=0.022) and caspase-3 was positively associated with caspase-8 expression (Spearman’s rho = 0.430, p=0.036). No other intercorrelations reached statistical significance. CONCLUSIONS: BCL2 and telomerase expression are correlated to critical parameters of malignant melanoma, affecting its biological behavior. Furthermore, down regulation of proteins such as caspase 3/8, which normally induce apoptosis, maybe is associated to resistance of applied chemotherapeutic strategies in this type of malignancy.

ΣΚΟΠΟΣ: Το κακόηθες μελάνωμα του δέρματος χαρακτηρίζεται από επιθετική βιολογική συμπεριφορά και χημειοαντοχή. Η διερεύνηση της απορρύθμισης του αποπτωτικού θανάτου και της τελομεράσης σε συσχέτιση με τη βιολογική συμπεριφορά του νεοπλάσματος αποτέλεσε στόχο της μελέτης. ΥΛΙΚΟ ΚΑΙ ΜΕΘΟΔΟΣ: Κατασκευάσθηκε με σύστημα ιστικών μικροσυστοιχιών ένας κύβος παραφίνης (διαμ. ιστικών κυλίνδρων 2mm) με εμφυτευμένο το σύνολο των περιστατικών (n=25). Εν συνεχεία, διενεργήθηκε, σε συνεχείς μικροτομηθείσες τομές, ανοσοϊστοχημικές έλεγχος για την ανάδειξη της έκφρασης των πρωτεινών caspase3, caspase8, bcl2 και h TERT. Τα αποτελέσματα της ανοσοϊστοχημείας ποσοτικοποιήθηκαν με την εφαρμογή ημιαυτόματου συστήματος ανάλυσης εικόνας, ενώ η στατιστική επεξεργασία πραγματοποιήθηκε με το λογισμικό SPSS (chi square test, interrater kappa). ΑΠΟΤΕΛΕΣΜΑΤΑ: Ανοσοϊστοχημική ισχυρή έκφραση παρατηρήθηκε…

Subjects/Keywords: Μελάνωμα; Τελομεράση; Απόπτωση; Κλινικές παράμετροι; Ιστικές μικροσυστοιχίες; Melanoma; Telomerase; Apoptosis; Caspase-3; Caspase-8; Bcl-2; Clinical parameters; Tissue microarrays

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ζυγούρης, . �. (2009). Μελέτη της έκφρασης της τελομεράσης στα μελανώματα σε συσχετισμό με κλινικές παραμέτρους και δείκτες απόπτωσης. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/24256

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ζυγούρης, Παναγιώτης. “Μελέτη της έκφρασης της τελομεράσης στα μελανώματα σε συσχετισμό με κλινικές παραμέτρους και δείκτες απόπτωσης.” 2009. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 07, 2020. http://hdl.handle.net/10442/hedi/24256.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ζυγούρης, Παναγιώτης. “Μελέτη της έκφρασης της τελομεράσης στα μελανώματα σε συσχετισμό με κλινικές παραμέτρους και δείκτες απόπτωσης.” 2009. Web. 07 Apr 2020.

Vancouver:

Ζυγούρης �. Μελέτη της έκφρασης της τελομεράσης στα μελανώματα σε συσχετισμό με κλινικές παραμέτρους και δείκτες απόπτωσης. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/10442/hedi/24256.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ζυγούρης �. Μελέτη της έκφρασης της τελομεράσης στα μελανώματα σε συσχετισμό με κλινικές παραμέτρους και δείκτες απόπτωσης. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. Available from: http://hdl.handle.net/10442/hedi/24256

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Ol, Kevser Kuşat. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması .

Degree: ESOGÜ, Tıp Fakültesi, Tıbbi Biyokimya, 2014, Eskisehir Osmangazi University

URL: http://hdl.handle.net/11684/892

► Bu çalışmada modifiye sıvı diyet aracılığıyla prenatal ve postnatal etanol maruziyeti oluşturulan rat yavrularının serebral korteksindeki nörodejenerasyonun etkisini incelemeyi ve betain ve omega-3 takviyesinin koruyucu… (more)

▼ Bu çalışmada modifiye sıvı diyet aracılığıyla prenatal ve postnatal etanol maruziyeti oluşturulan rat yavrularının serebral korteksindeki nörodejenerasyonun etkisini incelemeyi ve betain ve omega-3 takviyesinin koruyucu etkilerini göstermeyi amaçladık. Prenatal ve postnatal periyodda etanole maruz kalındığında yavrularda Fetal Alkol Sendromu (FAS) görülmektedir. Ratlar kontrol, etanol, etanol+omega-3, etanol+betain, etanol+betain+omega-3 şeklinde 5 gruba ayrıldı. Rat yavrularının serebral korteksindeki etanol indüksiyonuna bağlı değişiklerde betain ve omega-3 ün etkilerini incelemek amacıyla biyokimyasal olarak sitokrom c, kaspaz-3, kalpain, katepsin B ve L, DNA fragmantasyonu seviyeleri incelenmiş histolojik ve morfometrik bulgular elde edilmiştir. Etanol grubunda kaspaz-3, kalpain, katepsin B, sitokrom c seviyeleri kontrol grubu ile karşılaştırıldığında belirgin derecede artmıştır. Dahası, kaspaz-3 ve kalpain seviyeleri etanol+omega-3, etanol+betain, etanol+betain+omega-3 gruplarında etanol grubuyla karşılaştırıldığında azalmıştır. Aynı zamanda katepsin B düzeyleri etanol+betain grubunda kontrolden daha yüksektir. Etanol+betain+omega-3 grubunda katepsin B etanol ve etanol+betain gruplarıyla karşılaştırıldığında azalmıştır. Katepsin L ve DNA fragmantasyonunda istatistiksel olarak farklılık bulunamamıştır. Doku hasarı değerlendirmesi histolojik açıdan; kontrol, etanol+omega-3, etanol+betain, etanol+betain+omega-3 gruplarında hemoraji, PMNL, mikroglia etanol grubuyla karşılaştırıldığında istatistiksel olarak düşüktür. Aynı zamanda etanol+omega-3 ve kontrol gruplarında konjesyon ve nekroz etanol indüklü hasara göre azalış göstermiştir. Morfometrik analiz sonucu etanol alınımı etanol+omega-3 ve kontrol gruplarına göre nekrotik hücre sayısını artırmıştır. Sonuç olarak, postnatal etanol maruziyeti rat beyinlerinde nekrotik hücre ölümünü tetiklemektedir, omega-3 ve betain nörodejenerasyonu azaltmaktadır. Omega-3 ve betain özellikle FAS ın önlenmesinde, nörodejenerasyon açısından yararlı olabilir. Advisors/Committee Members: Kanbak, Güngör (advisor).

Subjects/Keywords: Betain; Fetal Alkol Sendromu (FAS); Kaspaz-3; Omega-3; Sitokrom c; Caspase-3; Betaine; Cytochrome c; Fetal Alcohol Syndrome (FAS)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ol, K. K. (2014). Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması . (Thesis). Eskisehir Osmangazi University. Retrieved from http://hdl.handle.net/11684/892

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ol, Kevser Kuşat. “Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması .” 2014. Thesis, Eskisehir Osmangazi University. Accessed April 07, 2020. http://hdl.handle.net/11684/892.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ol, Kevser Kuşat. “Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması .” 2014. Web. 07 Apr 2020.

Vancouver:

Ol KK. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması . [Internet] [Thesis]. Eskisehir Osmangazi University; 2014. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/11684/892.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ol KK. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması . [Thesis]. Eskisehir Osmangazi University; 2014. Available from: http://hdl.handle.net/11684/892

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

20. Burgess, Joshua T. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.

Degree: 2016, Queensland University of Technology

URL: http://eprints.qut.edu.au/98661/

► SASH1 (SAM and SH3 domain-containing protein 1) is a putative tumour suppressor functioning in the control of apoptosis and cellular proliferation. In summary this project… (more)

Subjects/Keywords: SASH1; Caspase 3; Chloropyramine; NF-κ; B; DNA Damage repair; hSSB1; Apoptosis; Breast Cancer; Lung Cancer; 14-3-3

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burgess, J. T. (2016). The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/98661/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burgess, Joshua T. “The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.” 2016. Thesis, Queensland University of Technology. Accessed April 07, 2020. http://eprints.qut.edu.au/98661/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burgess, Joshua T. “The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.” 2016. Web. 07 Apr 2020.

Vancouver:

Burgess JT. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. [Internet] [Thesis]. Queensland University of Technology; 2016. [cited 2020 Apr 07]. Available from: http://eprints.qut.edu.au/98661/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burgess JT. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. [Thesis]. Queensland University of Technology; 2016. Available from: http://eprints.qut.edu.au/98661/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Benetone, Maria Zilah. Apoptose e proliferação na placenta de búfalas.

Degree: Mestrado, Anatomia dos Animais Domésticos e Silvestres, 2005, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/ ;

► A apoptose é um processo fisiológico que desempenha papel crucial no desenvolvimento, remodelagem e senescência teciduais, inclusive placentários. A placenta, enquanto órgão temporário, atravessa todas… (more)

▼ A apoptose é um processo fisiológico que desempenha papel crucial no desenvolvimento, remodelagem e senescência teciduais, inclusive placentários. A placenta, enquanto órgão temporário, atravessa todas estas fases em aproximadamente 10 meses, na espécie bubalina. O crescimento da placenta e a nutrição fetal requerem altas taxas de renovação e diferenciação celulares, e a maturação placentária está relacionada à redução das células epiteliais das criptas carunculares maternas. As modificações morfológicas celulares decorrentes do processo de apoptose são fruto de eventos bioquímicos complexos promovidos por uma família de cisteína-proteases, as caspases, especialmente as caspases executoras, dentre as quais se destaca a caspase-3, capaz de degradar várias proteínas citoplasmáticas e nucleares. Durante a apoptose, ocorre a clivagem caspase-mediada da citoqueratina 18, proteína dos filamentos intermediários do citoesqueleto, e com isso a formação de um neoepítopo específico. Por meio de métodos imunoistoquímicos pode-se detectar a presença tanto deste neoepítopo, quanto da forma ativa da caspase-3, o que demonstra que a célula entrou em estágio irreversível de morte celular. Morfologicamente, algumas das principais alterações celulares observadas são condensação da cromatina, a degradação e fragmentação do DNA, a formação de ?blebs? (pregas/bolhas) na membrana plasmática, além da fragmentação celular em corpúsculos apoptóticos, os quais podem ser identificados em cortes corados pelo método de rotina hematoxilina e eosina, utilizando-se microscópio de imunofluorescência, devido à eosinofluorescência das células em apoptose. Assim como a apoptose, a proliferação celular participa no equilíbrio homeostático tissular. Neste estudo, pretende-se avaliar a ocorrência de apoptose e proliferação celular em 42 placentônios de diferentes animais em diversas fases gestacionais (2-10 meses de gestação), em tecidos fixados em 4% paraformoldeído, incluídos em paraplast e submetidos à imunoistoquímica (anticorpo monoclonal M30 CytoDeath; Caspase-3 Clivada; PCNA - antígeno de marcação nuclear), sendo também avaliada a presença de corpúsculos apoptóticos eosinofluorescentes nas amostras. Utilizando-se M30 e caspase-3 clivada pudemos constatar a ocorrência de apoptose nos epitélios uterino e trofoblástico, em células gigantes placentárias e, ocasionalmente, em células mesenquimais fetais, do estroma uterino e endoteliais. Não houve diferenças significativas (p<0.05) entre os métodos adotados, mas sim entre os estágios gestacionais. Para o M30, houve um aumento significante da apoptose do primeiro grupo (2-4.5 meses) em relação ao quarto grupo (9-10 meses); no caso da Caspase-3 Clivada houve um aumento estatísticamente significante (p<0.05) entre os três primeiros grupos (2-4.5; 5-6.5; e 7-8.5 meses de gestação, respectivamente) de animais em relação ao quarto grupo. Para o PCNA, ocorreu uma diminuição no número de células em proliferação dos dois primeiros grupos de animais em relação ao quarto grupo (p<0.05). A presença de corpúsculos… Advisors/Committee Members: Miglino, Maria Angélica.

Subjects/Keywords: Apoptose; Apoptosis; Búfalos; Buffalo; Caspase 3; Caspase-3; Placenta; Placenta; Proliferação; Proliferation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benetone, M. Z. (2005). Apoptose e proliferação na placenta de búfalas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/ ;

Chicago Manual of Style (16^th Edition):

Benetone, Maria Zilah. “Apoptose e proliferação na placenta de búfalas.” 2005. Masters Thesis, University of São Paulo. Accessed April 07, 2020. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/ ;.

MLA Handbook (7^th Edition):

Benetone, Maria Zilah. “Apoptose e proliferação na placenta de búfalas.” 2005. Web. 07 Apr 2020.

Vancouver:

Benetone MZ. Apoptose e proliferação na placenta de búfalas. [Internet] [Masters thesis]. University of São Paulo; 2005. [cited 2020 Apr 07]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/ ;.

Council of Science Editors:

Benetone MZ. Apoptose e proliferação na placenta de búfalas. [Masters Thesis]. University of São Paulo; 2005. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/ ;

22. Ressio, Rodrigo Albergaria. Avaliação da imuno-expressão de proteínas da via da apoptose mediadas pela proteína p53 no carcinoma hepatocelular.

Degree: Mestrado, Fisiopatologia Experimental, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5160/tde-03112010-165617/ ;

►

O presente estudo teve por objetivo estudar a participação da apoptose na carcinogênese hepatocelular, quantificando os corpos apoptóticos imunomarcados por caspase-3 clivada em amostras de… (more)

▼

O presente estudo teve por objetivo estudar a participação da apoptose na carcinogênese hepatocelular, quantificando os corpos apoptóticos imunomarcados por caspase-3 clivada em amostras de carcinoma hepatocelular (CHC) em pacientes com ou sem cirrose, comparando também estes achados com amostras correspondentes de fígado não tumoral. Visou também à análise semi-quantitativa da imuno-expressão da proteína p53, Bax e Citocromo-C, relacionadas à via mitocondrial da apoptose em busca de eventuais relações com as variáveis clínicopatológicas dos carcinomas hepatocelulares. A análise comparativa da distribuição das diversas proteínas aqui estudadas foi ainda efetuada, com vistas à possível demonstração de sua interação no processo de apoptose em CHC. Amostras selecionadas de 79 casos de CHC foram distribuídas em micromatriz tecidual e submetidas a pesquisa imuno-histoquímica com amplificação por polímeros curtos de dextran ligados a peroxidase. IA foi maior nos CHC que nas amostras não-neoplásicas, mostrando ainda tendência a associação com o grau histológico do CHC .A imuno-expressão de p53 foi maior nos CHC em fígado cirrótico (CHC-C), em casos com invasão vascular, e nos graus histológicos altos. Houve maior imunoexpressão de citocromo c em CHC-C, sendo importante sua associação com p53. Bax mostrou apenas tendência a associação com o tamanho do CHC. Essas evidências contribuem para a compreensão da importância da via mitocondrial da apoptose mediada pela proteína p53 no CHC, destacando também prováveis diferenças do mecanismo carcinogenético na presença ou não de cirrose

This study aimed at the assesment of aspects of the role of apoptosis in hepatocellular carcinogenesis, quantifying apoptotic bodies immunomarked by cleaved caspase-3 in samples of hepatocellular carcinoma (HCC) in patients with or without cirrhosis, further comparing these findings to those from samples in non-tumoral areas of these livers. We also aimed herein to semiquantitate the immunoexpression of p53, Bax, Cytochrome-C, participants of the mitochondrial pathway of apoptosis, searching for possible relations with clinico-pathological variables in HCC. Samples from 79 cases of HCC were arranged in tissue microarrays were and submitted to immunohistochemical reaction with signal amplification achieved by the short-polymer-peroxidase system. Apoptotic index measured by immunoexpression of cleaved-caspase 3 was higher in HCC than in samples from non-neoplastic areas. p53 immunoexpression was higher in HCC occurring in cirrhotic livers, (HCC-C), in cases with vascular invasion and in higher histological grades. Cytochrome-c immunoexpression was also higher in HCC-C and, interestingly, was directly related to p53. Bax immunoreactivity showed only a trend for a relation with the size of HCC. The evidences from the present study further demonstrate the importance of p53-mediated pathway of apoptosis in HCC, and also point for possible differences in carcinogenesis in cirrhotic versus non-cirrhotic livers

Advisors/Committee Members: Alves, Venancio Avancini Ferreira.

Subjects/Keywords: Apoptose; Apoptosis; Carcinoma hepatocelular; Caspase-3 clivada; Citocromo C; Cleaved caspase-3; Cytochrome C; Genes p53; Hepatocellular carcinoma; Immunohistochemistry; Imunoistoquímica; P53 gene

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ressio, R. A. (2010). Avaliação da imuno-expressão de proteínas da via da apoptose mediadas pela proteína p53 no carcinoma hepatocelular. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5160/tde-03112010-165617/ ;

Chicago Manual of Style (16^th Edition):

Ressio, Rodrigo Albergaria. “Avaliação da imuno-expressão de proteínas da via da apoptose mediadas pela proteína p53 no carcinoma hepatocelular.” 2010. Masters Thesis, University of São Paulo. Accessed April 07, 2020. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-03112010-165617/ ;.

MLA Handbook (7^th Edition):

Ressio, Rodrigo Albergaria. “Avaliação da imuno-expressão de proteínas da via da apoptose mediadas pela proteína p53 no carcinoma hepatocelular.” 2010. Web. 07 Apr 2020.

Vancouver:

Ressio RA. Avaliação da imuno-expressão de proteínas da via da apoptose mediadas pela proteína p53 no carcinoma hepatocelular. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2020 Apr 07]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5160/tde-03112010-165617/ ;.

Council of Science Editors:

Ressio RA. Avaliação da imuno-expressão de proteínas da via da apoptose mediadas pela proteína p53 no carcinoma hepatocelular. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/5/5160/tde-03112010-165617/ ;

23. Raquel Autran Coelho. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.

Degree: 2008, Universidade Federal de São Paulo

URL: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563

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Objetivos: Estudar a expressao imuno-histoquimica de topoisomerase II e de caspase-3 ativada, marcadores de proliferacao e de apoptose, respectivamente, a deteccao de DNA HPV e… (more)

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Objetivos: Estudar a expressao imuno-histoquimica de topoisomerase II e de caspase-3 ativada, marcadores de proliferacao e de apoptose, respectivamente, a deteccao de DNA HPV e a evolucao da lesao cervical em mulheres portadoras de lesao intra-epitelial escamosa de baixo grau (LBG). Metodos: Foram avaliadas 40 mulheres portadoras de LBG e 32 sem neoplasia cervical, diagnosticadas por exame cito-colpo-histopatologico, quanto a imunoexpressao de topoisomerase II e de caspase-3 ativada e quanto a deteccao de DNA HPV por PCR consensual (GP5+/GP6+) em material de esfregaco cervico-vaginal. Os achados foram relacionados as variaveis clinicas das pacientes e a evolucao clinica das lesoes cervicais em 12 meses. As pacientes assinaram termo de consentimento livre e esclarecido. Resultados: A media percentual de celulas imunomarcadas por topoisomerase foi de 11,71% e 4,13%, no grupo com LBG e controle, respectivamente, com diferenca estatisticamente significante. Observou-se que houve expressao de caspase-3 em 17 (42,5%) e em 5 (15,63%) pacientes com e sem LBG, respectivamente, com diferenca estatisticamente significante. Foi detectado HPV DNA em 65% das pacientes com LBG e em 59,4% das pacientes sem lesao cervical, sem relacao com a expressao de topoisomerase II ou caspase-3. Na presenca de DNA-HPV, a expressao de topoisomerase II no grupo com LBG foi significativamente maior do que em fragmentos sem lesao. Nao foi observada diferenca quanto a evolucao da lesao cervical em 12 meses de acordo com a imunoexpressao de topoisomerase II. Com relacao a caspase-3 ativada, a maioria das pacientes com imuno-histoquimica negativa teve regressao da lesao cervical. Conclusoes: A imunoexpressao de topoisomerase II e de caspase-3 ativada podem ser considerados marcadores de proliferacao e de apoptose em lesao cervical de baixo grau, sem relacao com a presenca de DNA-HPV.

Purpose: To evaluate the correlation between the expression of topoisomerase II alpha, active caspase-3 and infection with human papillomavirus in low-grade cervical intraepithelial lesion and in the normal cervix, and whether they might influence susceptibility to, or evolution of, cervical lesion. Patients and methods: Forty cervical biopsies patients with low-grade cervical intraepithelial lesion and thirty-two with normal cervix were stained by immunohistochemistry for topoisomerase IIá and active caspase-3 and were investigated for the presence of HPV on exfoliated cells by general primer GP5+/6+ PCR amplification of DNA. These findings were correlated with clinicopathological features of the patients including their clinical outcome after twelve months. Subjects provided written informed consent. Results: Low-grade CIN patients as a group had a significantly higher expression of topoisomerase II alpha compared to controls, without correlation to disease outcome at 12 months. Caspase-3 was expressed in 42.5% of CIN patients and in 15.63% without disease, and most of women without caspase-3 receded cervical lesion. HPV DNA testing was positive in 65% of the patients…

Advisors/Committee Members: Ismael Dale Cotrim Guerreiro da Silva, Neila Maria de Góis Speck, Gustavo Rubino de Azevedo Focchi, Julisa Chamorro Lascasas Ribalta, José Eleutério Junior, Roberto Zamith.

Subjects/Keywords: DNA topoisomerases tipo II; Caspase 3; Neoplasia intraepitelial cervical; Infecções por papilomavírus; GINECOLOGIA E OBSTETRICIA; DNA topoisomerases type II; Caspase 3; Cervical intraepithelial neoplasia; Papillomavirus infections

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Coelho, R. A. (2008). Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. (Thesis). Universidade Federal de São Paulo. Retrieved from http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Coelho, Raquel Autran. “Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.” 2008. Thesis, Universidade Federal de São Paulo. Accessed April 07, 2020. http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Coelho, Raquel Autran. “Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.” 2008. Web. 07 Apr 2020.

Vancouver:

Coelho RA. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. [Internet] [Thesis]. Universidade Federal de São Paulo; 2008. [cited 2020 Apr 07]. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Coelho RA. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. [Thesis]. Universidade Federal de São Paulo; 2008. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Παπαδόπουλος, Γεώργιος. Η επίδραση του αποκλεισμού των α1-αδρενεργικών υποδοχέων στην έκφραση των κασπασών στον κοιλιακό προστάτη του αρουραίου.

Degree: 2009, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/23799

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INTRODUCTION - OBJECTIVES Autonomic sympathetic innervation of prostatic smooth muscles is mediated by α-adrenergic receptors. Terazosin represents a widely-used α1-adrenergic antagonist which exerts its effect… (more)

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INTRODUCTION - OBJECTIVES Autonomic sympathetic innervation of prostatic smooth muscles is mediated by α-adrenergic receptors. Terazosin represents a widely-used α1-adrenergic antagonist which exerts its effect on prostatic stroma as well as on bladder neck. It causes smooth muscle relaxation and reduces the “dynamic” element of bladder outlet obstruction. Over the past decade it has been postulated that the above drug also induces apoptosis in prostatic cells potentially via its quinazoline-dependent activation of the transforming growth factor (TGF)-beta signal transduction pathway. In the present study we examined the effect of terazosin on the expression of caspases -1 and -3, which play a predominant role in the apoptotic cascade, in rat ventral epithelial cells. MATERIALS - METHODS Twenty-two 100 ± 5 day-old male Wistar rats were randomly allocated into two groups. One group (group A', 12 rats) received treatment with terazosin hydrochloride (Hytrin®, Abbott) (1.2 mg/Kg body weight every alternate day) through a specially modified oesophageal catheter for 120 days . Control subjects (group B', 10 animals) received the same amount of normal saline. Within 24 hours after the end of the experiment either terazosin or normal saline were discontinued and, subsequently, rats were sacrificed. Ventral prostate glands were, thereafter, dissected and weighed. Each gland was fixed in 10% buffered formalin and subsequently, fixed tissues were embedded in paraffin and assessed histologically as well as immunohistochemically using anti-caspase-1 and -3 polyclonal antibodies and a standard avidin-biotin immunoperoxidase method in sections of the ventral prostate. RESULTS All rats remained healthy throughout the course of the treatment. No terazosin-related side effects were observed. All animals showed the expected continuous increase in their body weights (p<0.001). There was no statistically significant difference in either absolute or relative ventral prostate weight between the two groups in both beginning and finishing the experiment (p>0.1). Moreover, terazosin treatment did not result in any macroscopic or microscopic alterations in prostate morphology. Caspase-1 and -3 expression were weak in both groups, however, more intense in terazosin group compared to controls (p<0.001). CONCLUSIONS Terazosin treatment enhances both caspase-1 and caspase-3 expression in rat ventral prostatic epithelial cells. It seems that these enzymes play a pivotal role in apoptotic process that is induced by quinazoline-based α1-adrenergic antagonists.

ΕΙΣΑΓΩΓΗ - ΣΚΟΠΟΣ Η αυτόνομη συμπαθητική νεύρωση των λείων μυών του προστάτη επιτυγχάνεται μέσω των α-αδρενεργικών υποδοχέων. Η Τεραζοσίνη συνιστά έναν ευρέως χρησιμοποιούμενο α1-αδρενεργικό αναστολέα ο οποίος ασκεί τη δράση του τόσο στο στρώμα του προστάτη όσο και στον αυχένα της ουροδόχου κύστεως. Προκαλεί χάλαση των λείων μυϊκών ινών στις περιοχές αυτές, με αποτέλεσμα τη μείωση του μυϊκού τόνου και την ανακούφιση από το λεγόμενο “δυναμικό” στοιχείο της υποκυστικής απόφραξης. Την…

Subjects/Keywords: α-αδρενεργικοί ανταγωνιστές; Τεραζοσίνη; Κασπάση-1; Κασπάση-3; Απόπτωση; Αρουραίοι; Προστάτης; Alpha-adrenoreceptor antagonists; Terazosin; Caspase-1; Caspase-3; Apoptosis; Rats; Prostate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Παπαδόπουλος, . �. (2009). Η επίδραση του αποκλεισμού των α1-αδρενεργικών υποδοχέων στην έκφραση των κασπασών στον κοιλιακό προστάτη του αρουραίου. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/23799

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Παπαδόπουλος, Γεώργιος. “Η επίδραση του αποκλεισμού των α1-αδρενεργικών υποδοχέων στην έκφραση των κασπασών στον κοιλιακό προστάτη του αρουραίου.” 2009. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 07, 2020. http://hdl.handle.net/10442/hedi/23799.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Παπαδόπουλος, Γεώργιος. “Η επίδραση του αποκλεισμού των α1-αδρενεργικών υποδοχέων στην έκφραση των κασπασών στον κοιλιακό προστάτη του αρουραίου.” 2009. Web. 07 Apr 2020.

Vancouver:

Παπαδόπουλος �. Η επίδραση του αποκλεισμού των α1-αδρενεργικών υποδοχέων στην έκφραση των κασπασών στον κοιλιακό προστάτη του αρουραίου. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/10442/hedi/23799.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Παπαδόπουλος �. Η επίδραση του αποκλεισμού των α1-αδρενεργικών υποδοχέων στην έκφραση των κασπασών στον κοιλιακό προστάτη του αρουραίου. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2009. Available from: http://hdl.handle.net/10442/hedi/23799

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Johannes Gutenberg Universität Mainz

25. Golbs, Antje. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.

Degree: 2008, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/

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Die Apoptose spielt eine entscheidende Rolle während der normalen Entwicklung des zentralen Nervensystems. Elektrische Aktivität und die Versorgung mit trophischen Faktoren sind ausschlaggebend für das… (more)

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Die Apoptose spielt eine entscheidende Rolle während der normalen Entwicklung des zentralen Nervensystems. Elektrische Aktivität und die Versorgung mit trophischen Faktoren sind ausschlaggebend für das Überleben von Neuronen. Um zu untersuchen, welche zellulären Prozesse die aktivitätsabhängige Apoptose in organotypischen Schnittkulturen des neugeborenen Neokortex beeinflussen, wurde in der vorliegenden Arbeit immunzytochemisch das Auftreten aktivierter Caspase-3, nach pharmakologischer Beeinflussung von Ionenkanälen und membranständigen Rezeptoren analysiert. Die Unterdrückung neuronaler Aktivität durch den Natriumionenkanalblocker TTX führte zu einem signifikanten Verlust kortikaler Neuronen. Ein ähnlicher Anstieg der Zahl apoptotischer Neurone konnte durch Applikation von Antagonisten ionotroper Glutamatrezeptoren, GABAA-Rezeptoren oder neuronaler Gap Junctions induziert werden. Jedoch konnte bei einigen Antagonisten die apoptosefördernde Wirkung erst nach längerer Einwirkung beobachtet werden. Im Weiteren wurde eine Methode etabliert, mit deren Hilfe eine Echtzeitanalyse der Apoptose kortikaler Neurone unter dem Entzug trophischer Faktoren in Gegenwart unterschiedlicher extrazellulärer Kaliumkonzentrationen ermöglicht wurde. Dazu wurden dissoziierte kortikale Kulturen mit dem pCaspase3-sensor Vektor transfiziert. Das durch dieses Plasmid codierte fluoreszente Protein wird Caspase-3 abhängig gespalten. In der vorliegenden Arbeit konnte gezeigt werden, dass der Caspase3-sensor spezifisch für die Aktivierung der Caspase-3 ist, und dass die Überlebensfähigkeit der transfizierten Neurone durch das Transfektionsprotokoll nicht beeinflusst wird.

Apoptosis is an essential process during normal development of the brain. Electrical activity and supply with survival factors play a major role in regulating neuronal survival. I aimed at correlating neuronal activity and apoptosis in the developing murine neocortex. Therefore organotypic slice cultures of newborn mouse cerebral cortex were treated with antagonists of different ligand-gated receptors and voltage-dependent ion channels. Apoptosis was assessed using an antibody specific for the activated form of caspase-3. Blocking network activity by the sodium channel blocker TTX caused a significant loss of cortical neurons. Moreover application of antagonists of ionotropic glutamate receptors, GABAA receptors or neuronal gap junctions resulted in a similar increase in the number of apoptotic neurons. However some antagonists increased neuronal loss only after prolonged exposure. In a further approach an assay was established which involves the transfection of dissociated cultures of the newborn mouse neocortex with the pCaspase3-sensor vector encoding a protein sensitive to cleavage by caspase-3. With this assay the impact of different extracellular potassium concentrations on caspase-3 dependent cortical apoptosis following trophic deprivation should be elucidated. The method was found to be specific for caspase 3 activation and did not interfere with the viability of…

Subjects/Keywords: Apoptose, elektrische Aktivität, Caspase-3, Neuronen, neuronale Entwicklung, Neokortex, Neurotrophine, Calcium, Kalium; apoptosis, electrical activity, caspase-3, neuron, neuronal development, neocortex, neurotrophins, calcium, potassium; Natural sciences and mathematics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Golbs, A. (2008). Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/

Chicago Manual of Style (16^th Edition):

Golbs, Antje. “Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.” 2008. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed April 07, 2020. http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/.

MLA Handbook (7^th Edition):

Golbs, Antje. “Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.” 2008. Web. 07 Apr 2020.

Vancouver:

Golbs A. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2008. [cited 2020 Apr 07]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/.

Council of Science Editors:

Golbs A. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2008. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/

26. Champagne, Julien. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.

Degree: Docteur es, Biologie Santé, 2018, Montpellier

URL: http://www.theses.fr/2018MONTT018

► Chez la femme, le cancer du sein est le cancer le plus fréquemment diagnostiqué. Différents traitements sont disponibles selon le sous-type tumoral. Cependant, certaines patientes… (more)

▼ Chez la femme, le cancer du sein est le cancer le plus fréquemment diagnostiqué. Différents traitements sont disponibles selon le sous-type tumoral. Cependant, certaines patientes sont réfractaires à ces thérapies et restent vulnérables lors de récidives. Le cancer a longtemps été défini par une division aberrante des cellules, mais aujourd'hui, il est évident que la résistance à la mort cellulaire programmée est un paramètre majeur dans l'étiologie de la maladie.Les cyclines de type D régulent le cycle cellulaire en permettant la transition de la phase G1 à la phase S. Pour cela, elles activent les kinases dépendantes des cyclines 4/6 (CDK4/6) qui phosphorylent les protéines du rétinoblastome ce qui libère le facteur de transcription E2F. La Cycline D1 (CycD1) nucléaire est donc centrale dans le contrôle du cycle. Son gène est amplifié dans les cancers humains et la moitié des patientes atteintes d'un cancer du sein ont une surexpression de CycD1. Par l’activation de CDK4, CycD1 est essentielle à l'apparition et à la progression tumorale. Ainsi, des inhibiteurs spécifiques de CDK4/6 ont été développés contre le cancer du sein. Malheureusement, certaines patientes restent insensibles à ce traitement. À ce titre, le ciblage spécifique de CycD1 pourrait représenter une alternative clinique. En effet, en plus de la régulation du cycle, CycD1 est également impliquée, indépendamment de CDK4, dans la survie des cellules cancéreuses. Cependant, aucun mécanisme de l'impact de CycD1 dans le maintien tumoral n'a été établi pour démontrer ce potentiel thérapeutique. En outre, CycD1 a été décrite dans les organes à l’âge adulte pour réguler le métabolisme du glucose et l'hématopoïèse. Par conséquent, pour éviter tout effet secondaire indésirable, nous avons décidé d’évaluer l’implication potentielle de CycD1 dans les organes adultes. Grâce au Tandem-HTRF, basé sur le transfert d'énergie entre deux anticorps, nous avons révélé la dynamique inattendue de CycD1 dans chaque organe adulte. De plus, nous avons montré que l’altération de l'expression de CycD1 conduit à une diminution des capacités de survie des cellules saines post-mitotiques.Au vu de ces limitations, nous avons développé une nouvelle approche d'ARN interférence spécifique des cellules cancéreuses appelée TAG-RNAi. Cette technologie permet de cibler CycD1 uniquement dans la tumeur afin d'épargner les cellules saines. Cette approche innovante consiste à cibler un tag présent uniquement sur l’ARNm de CycD1 des cellules cancéreuses. Ainsi, nous avons découvert que le ciblage spécifique de CycD1 induit une régression rapide et spontanée des tumeurs dépendantes des oncogènes RAS ou ERBB2. Par protéomique in vivo, j'ai découvert que lors de stress pro-apoptotiques, CycD1 cytoplasmique interagit avec la procaspase-3 et bloque son activation pour empêcher l'apoptose des cellules. Ces travaux démontrent la valeur clinique du ciblage spécifique de CycD1 dans les cancers afin d'améliorer l'efficacité des chimiothérapies.Par conséquent, il restait à déterminer comment appliquer le… Advisors/Committee Members: Pin, Jean-Philippe (thesis director).

Subjects/Keywords: Cancer du sein; Cycline D1; Apoptose; Caspase-3; Thérapie à la carte; ARN interférence; Breast cancer; Cyclin D1; Apoptosis; Caspase-3; Personalized medecine; RNA interference

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Champagne, J. (2018). Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2018MONTT018

Chicago Manual of Style (16^th Edition):

Champagne, Julien. “Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.” 2018. Doctoral Dissertation, Montpellier. Accessed April 07, 2020. http://www.theses.fr/2018MONTT018.

MLA Handbook (7^th Edition):

Champagne, Julien. “Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.” 2018. Web. 07 Apr 2020.

Vancouver:

Champagne J. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. [Internet] [Doctoral dissertation]. Montpellier; 2018. [cited 2020 Apr 07]. Available from: http://www.theses.fr/2018MONTT018.

Council of Science Editors:

Champagne J. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. [Doctoral Dissertation]. Montpellier; 2018. Available from: http://www.theses.fr/2018MONTT018

University of Pretoria

27. Patrick, Sean Mark. Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays.

Degree: Physiology, 2009, University of Pretoria

URL: http://hdl.handle.net/2263/26120

► Endocrine disrupting chemicals (EDCs) such as DDT have the ability to disrupt hormonally controlled processes, such as spermatogenesis, which is the maturation of germ cells… (more)

▼ Endocrine disrupting chemicals (EDCs) such as DDT have the ability to disrupt hormonally controlled processes, such as spermatogenesis, which is the maturation of germ cells into spermatozoa. During normal spermatogenesis, germ cell apoptosis can occur, but the degree of apoptosis within the testis could possibly be affected by exposure to EDCs. In 2004, a pilot study on the reproductive health of two freshwater fish species, Oreochromis mossambicus and Clarias gariepinus, from three impoundments in the Luvuvhu River, found concerning levels of DDT and its metabolites in both species from the Nandoni Dam, and in O. mossambicus from the Xikundu Weir. This was not surprising as a large part of the Luvuvhu River catchment is located within an area where ongoing DDT-spraying occurs for vector control purposes. Hence, in 2006, a larger WRC-funded project began to further investigate the findings from the pilot study. A subsidiary study, spanning two seasons, was initiated to investigate testicular apoptosis in fish from the polluted systems, the Nandoni Dam (ND) and the Xikundu Weir (XW), as well as a reference site, the Albasini Dam (AD), utilizing caspase-3 and TUNEL immunoexpression as apoptotic markers. In addition, three fixatives, Bouin’s Fluid (BF), Neutrally Buffered Formalin (NBF) and Paraformaldehyde (PFA), were used to determine which would be the optimal fixative for both histological and immunohistochemical assessments. Sampling occurred during season 1, the low-flow season (October 2007), during DDT spraying of the surrounding area, and season 2, the high–flow season (February 2008), two months after the DDT-spraying was completed. The testes of O. mossambicus (n = 19 season 1, n = 25 season 2) and C. gariepinus (n = 19 season 1, n = 20 season 2) were fixed in the above-mentioned fixatives, embedded in paraffin wax, prepared for immunohistochemistry, and exposed to caspase-3 antibodies and TUNEL antibodies individually. The results indicated that the residues of p,p´-DDT - DDD and - DDE were found in the fat samples of both O. mossambicus and C. gariepinus, in AD, ND and XW. Testicular apoptotic assessment using the caspase-3 assay clearly labeled spermatocytes in the process of cellular death in both seasons, in all three fixatives. When comparing the two assays, a significant difference is found between the caspase-3 and TUNEL positive cells. The results further show that, when comparing the three sampling sites, the highest amount of positive cells are found at the XW. The decrease observed in season two, in both the caspase-3 and TUNEL assay may possibly be linked to the stage of spermatogenesis, coinciding with hormonal changes associated with the different sampling seasons (i.e. breeding and non-breeding seasons). The levels of DDT found in the fat tissue, could not be correlated to an up-regulation in apoptotic cells. The results The results indicated that the choice of fixative, could affect the identification of the amount of positive cells. The utility of the caspase-3 and TUNEL assays, in… Advisors/Committee Members: Dr J C Van Dyk (advisor), Prof M S Bornman (advisor).

Subjects/Keywords: Apoptosis; Caspase-3 immunohistochemistry; Tunel; Oreochromis mossambicus; Clarias gariepinus; Spermatogenesis; Ddt; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Patrick, S. M. (2009). Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/26120

Chicago Manual of Style (16^th Edition):

Patrick, Sean Mark. “Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays.” 2009. Masters Thesis, University of Pretoria. Accessed April 07, 2020. http://hdl.handle.net/2263/26120.

MLA Handbook (7^th Edition):

Patrick, Sean Mark. “Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays.” 2009. Web. 07 Apr 2020.

Vancouver:

Patrick SM. Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/2263/26120.

Council of Science Editors:

Patrick SM. Testicular apoptotic activity in two bio-sentinel fish species inhabiting an aquatic ecosystem in an area where continual DDT spraying occurs : utility of immunohistochemical assays. [Masters Thesis]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/26120

Universidade do Rio Grande do Sul

28. Fabres, Rafael Bandeira. Papel da progesterona como possível neuroprotetor em modelo de hipóxia-isquemia encefálica neonatal.

Degree: 2016, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/165216

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A encefalopatia hipóxico-isquêmica neonatal, ou simplesmente hipóxia-isquemia (HI) neonatal, é uma das principais causas de morbidade e mortalidade em neonatos humanos. De 20% a 50%… (more)

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A encefalopatia hipóxico-isquêmica neonatal, ou simplesmente hipóxia-isquemia (HI) neonatal, é uma das principais causas de morbidade e mortalidade em neonatos humanos. De 20% a 50% dos recém-nascidos com HI severa morrem no período perinatal. Quando sobrevivem, 25% apresentam deficiências neuropsicológicas, como dificuldade de aprendizado, epilepsia e paralisia cerebral. Devido a isso, a eficácia de possíveis agentes neuroprotetores tem sido testada em modelos animais. Há razão para se pensar que a progesterona tem um forte potencial para o tratamento da HI neonatal, já que a sua utilização tem se mostrado benéfica em pesquisas relacionadas com lesão cerebral traumática, lesão cerebral isquêmica e outros modelos de lesão do sistema nervoso central (SNC) em adultos. Inúmeros estudos têm mostrado que o modelo animal de HI de Rice e Vannucci (1981) em animais neonatos, utilizado no presente trabalho, pode produzir lesões no sistema nervoso central relativamente previsíveis, e que estas lesões encefálicas parecem semelhantes às observadas clinicamente em humanos (SALMASO et al., 2014). Para a realização do modelo de HI foram utilizados ratos Wistar com idade de 7 dias (P7). Após a oclusão da carótida esquerda, os animais foram colocados em câmaras para exposição à atmosfera hipóxica com 8% O2/92% N2 por 90 minutos. Os animais foram divididos em cinco grupos experimentais: SHAM, HI, HI+PROG-PRÉ (PRÉ), HI+PROG-PÓS (PÓS), HI+PROG-PRÉ/PÓS (PP). Os termos PRÉ e PÓS referem-se à administração de progesterona (na dose de 5 mg/kg) antes ou após o procedimento de HI neonatal . Dependendo do grupo experimental, os animais foram tratados com progesterona imediatamente antes da isquemia e/ou 6 e 24 horas após o início da hipóxia. Foram analisados o peso corporal dos animais (imediatamente antes da isquemia e 6, 24 e 48 horas após o início da hipóxia), o volume de lesão cerebral, além da expressão das proteínas p-Akt e caspase-3 pela técnica de Western blotting.

Neonatal hypoxic-ischemic encephalopathy or simply neonatal hypoxia-ischemia (HI) is a main cause of morbidity and mortality in human neonates. Moreover, 25% of survivors show neuropsychological dysfunctions such as learning difficulties, epilepsy and cerebral palsy. Because of this, the effectiveness of potential neuroprotective agents has been tested in animal models. There is a reason to suppose that progesterone has a strong potential for the treatment of neonatal HI since its use has been shown to be beneficial in researches related to traumatic brain injury, ischemic brain injury and other central nervous system injury models (CNS) in adults. Several studies have shown that the newborn animal model of HI developed by Rice and Vannucci (1981), and used in the present study, can produce lesions in the central nervous system which are predictable and similar to those observed clinically in humans. In order to perform the HI model we used 7 days old (P7) Wistar rats. After occlusion of the left carotid, the animals were placed in hypoxic chambers and exposed to the…

Advisors/Committee Members: Ribeiro, Maria Flavia Marques.

Subjects/Keywords: Neonatal hypoxic-ischemic; Hipóxia-isquemia encefálica; Akt; Progesterona; Apoptose; Lesion volume; Caspase 3; Progesterone

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fabres, R. B. (2016). Papel da progesterona como possível neuroprotetor em modelo de hipóxia-isquemia encefálica neonatal. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/165216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fabres, Rafael Bandeira. “Papel da progesterona como possível neuroprotetor em modelo de hipóxia-isquemia encefálica neonatal.” 2016. Thesis, Universidade do Rio Grande do Sul. Accessed April 07, 2020. http://hdl.handle.net/10183/165216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fabres, Rafael Bandeira. “Papel da progesterona como possível neuroprotetor em modelo de hipóxia-isquemia encefálica neonatal.” 2016. Web. 07 Apr 2020.

Vancouver:

Fabres RB. Papel da progesterona como possível neuroprotetor em modelo de hipóxia-isquemia encefálica neonatal. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2016. [cited 2020 Apr 07]. Available from: http://hdl.handle.net/10183/165216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fabres RB. Papel da progesterona como possível neuroprotetor em modelo de hipóxia-isquemia encefálica neonatal. [Thesis]. Universidade do Rio Grande do Sul; 2016. Available from: http://hdl.handle.net/10183/165216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

29. Malavez, Yadira. Mechanisms of Caspase-3 Regulation in the Execution of Cell Death.

Degree: PhD, Molecular, Cellular and Developmental Biology, 2012, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1331142346

► Apoptosis is an evolutionarily conserved mechanism necessary for the homeostasis in multicellular organisms. The cysteine protease caspase-3 has a key role in apoptosis for… (more)

Subjects/Keywords: Molecular Biology; Apoptosis; Cell death; Caspase-3; PKCd; Proein Kinase C delta; phosphorylation; mutagenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Malavez, Y. (2012). Mechanisms of Caspase-3 Regulation in the Execution of Cell Death. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1331142346

Chicago Manual of Style (16^th Edition):

Malavez, Yadira. “Mechanisms of Caspase-3 Regulation in the Execution of Cell Death.” 2012. Doctoral Dissertation, The Ohio State University. Accessed April 07, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1331142346.

MLA Handbook (7^th Edition):

Malavez, Yadira. “Mechanisms of Caspase-3 Regulation in the Execution of Cell Death.” 2012. Web. 07 Apr 2020.

Vancouver:

Malavez Y. Mechanisms of Caspase-3 Regulation in the Execution of Cell Death. [Internet] [Doctoral dissertation]. The Ohio State University; 2012. [cited 2020 Apr 07]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1331142346.

Council of Science Editors:

Malavez Y. Mechanisms of Caspase-3 Regulation in the Execution of Cell Death. [Doctoral Dissertation]. The Ohio State University; 2012. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1331142346

Universidade de Brasília

30. Sílvia Taveira Elias. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.

Degree: 2009, Universidade de Brasília

URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575

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O câncer é um problema mundial de saúde pública. Nas últimas décadas a incidência de câncer de cabeça e pescoço vem aumentando, sendo os países… (more)

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O câncer é um problema mundial de saúde pública. Nas últimas décadas a incidência de câncer de cabeça e pescoço vem aumentando, sendo os países desenvolvidos os mais afetados. As terapias convencionais para este tipo de câncer, cirurgia e radioterapia, são agressivas e não aumentam de maneira considerável a qualidade e expectativa de vida dos pacientes. Nesse cenário, a descoberta de novas terapias é necessária. Esse trabalho tem como objetivo investigar o efeito citotóxico do extrato bruto de tabaco (EBT) e cinco frações provenientes dele (metanólica, neutra, carbônica, ácida e básica) em células de carcinoma oral humano. A exposição das células de câncer oral ao EBT induz a morte celular e diminui a viabilidade celular de maneira dose-dependente. Das frações testadas, apenas a neutra foi capaz de induzir uma significante morte celular (acima de 50%) após 48 horas de tratamento. Ademais, fragmentação do DNA e ativação da caspase-3 indicam que a morte celular induzida por EBT e pela fração neutra tem natureza apoptótica. Os resultados mostram que apesar do tabaco ser um carcinógeno conhecido, possuir inúmeros componentes indutores de tumor, ele também pode conter componentes capazes de induzir morte em células de câncer. Pelo fato da fração neutra ser capaz de também induzir morte celular, pode-se inferir que o componente apoptótico é apolar. Novos estudos são necessários para avaliar a estrutura química envolvida neste processo.

Cancer is a public health problem worldwide. Incidences of head and neck cancer are increasing in the last decades, and the developed countries are the most affected. Current therapeutic options for this type of cancer are aggressive, including surgery and radiotherapy. In addition, they have not yet translated into an improvement of life quality or expectancy to patients. In this scenario, new therapeuticals are urgently needed and actively sought after. The goal of this study was to investigate the cytotoxic effects of tobacco crude extract (TCE) and 5 fractions thereof (methanolic, neutral, carbonic, acid and basic) on a human oral squamous cell carcinoma. Exposure of oral cancer cells to TCE induced cell death and decreased cell viability in a dose-dependent manner. Out of the tested fractions, only the neutral one was able to induce significant cell death (over 50%) over the period of 48h. In addition, DNA laddering and caspase-3 activation indicate that the cell death processes induced by TCE and neutral fraction were apoptotic in nature. Our results indicate that although tobacco is a known carcinogen, possessing many tumorinitiating compounds, it could also contain compounds that are useful to induce apoptosis in cancer cells. Because the neutral fraction was also able to induce apoptosis, it is postulated that this putative compound is non-polar, although further investigation is needed to uncover its true nature and chemical structure.

Advisors/Committee Members: Eliete Neves da Silva Guerra, Marie Togashi, Andrea Barreto Motoyama, Fabio Pittella Silva.

Subjects/Keywords: apoptose; extrato bruto de tabaco; caspase-3; CIENCIAS DA SAUDE; câncer de boca

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Elias, S. T. (2009). Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elias, Sílvia Taveira. “Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.” 2009. Thesis, Universidade de Brasília. Accessed April 07, 2020. http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elias, Sílvia Taveira. “Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.” 2009. Web. 07 Apr 2020.

Vancouver:

Elias ST. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. [Internet] [Thesis]. Universidade de Brasília; 2009. [cited 2020 Apr 07]. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elias ST. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. [Thesis]. Universidade de Brasília; 2009. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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