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University of Edinburgh
1.
Mackay, Martha.
The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.
Degree: PhD, 2014, University of Edinburgh
URL: http://hdl.handle.net/1842/17972
► The design and development of fluorescent reporters focussed on highly sensitive, specific, and selective imaging of cancer targets is described. These novel optical molecular probes…
(more)
▼ The design and development of fluorescent reporters focussed on highly sensitive, specific, and selective imaging of cancer targets is described. These novel optical molecular probes were synthesised with the aim of creating bio-imaging breakthroughs that will aid the clinical analysis of cancer. A specific target of the project was to develop fluorescent reporters for Caspases; intracellular endopeptidases that play an essential role in apoptosis. Lack of activation of the ‘Caspase Cascade’ causes uncontrolled proliferation of cells and has been deemed a ‘Hallmark of Cancer’. In particular, low Caspases-3/7 activities have been associated with a range of cancers, thus molecular detection of Caspases-3/7 activities could therefore lead to advances in oncology. A 14-member FRET library, based upon Caspases-3/7 specific peptide sequences, was initially developed. The cleavage rates and KM values were evaluated for Caspases-3/7, along with the cleavage rates for Cathepsin B, to determine the peptide with the greatest affinity and specificity for Caspase-3. Also developed was a set of internally quenched activity based molecular reporters constructed by attaching fluorophores to a tribranched dendron through the Caspase specific peptide, developed from the FRET Library. The KM values of the dendron probes with Caspase-3 were also evaluated. Furthermore, the dendron reporters were attached to cell penetrating peptides to enable delivery to intracellular Caspase and allow in situ detection of activated Caspase-3 within live cells. In addition, a new labelling moiety was developed enabling dual detection of reporters through fluorescence and MRI imaging. To achieve this, a perfluoro tag (C8F17) was tethered to a Cy5 dye to enable dual detection. The dual 19F-MRI/Cy5 dye was conjugated onto to a cell penetrating peptide to enable in vivo detection of the probe by 19F-MRI and fluorescent imaging.
Subjects/Keywords: 616.99; Caspase-3; molecular detection
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
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APA (6th Edition):
Mackay, M. (2014). The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/17972
Chicago Manual of Style (16th Edition):
Mackay, Martha. “The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed April 22, 2021.
http://hdl.handle.net/1842/17972.
MLA Handbook (7th Edition):
Mackay, Martha. “The development of fluorescent probes targeting Caspase-3 for detecting apoptosis.” 2014. Web. 22 Apr 2021.
Vancouver:
Mackay M. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/1842/17972.
Council of Science Editors:
Mackay M. The development of fluorescent probes targeting Caspase-3 for detecting apoptosis. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/17972

Univerzitet u Beogradu
2.
Dožić, Branko S., 1968-.
Imunohistohemijska analiza i prognostički značaj markera
unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu
pljuvačnih žlezda.
Degree: Medicinski fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get
► Medicina / Medicine
Adenoidni cistični karcinom (ACC), je jedan od najčešćih malignih tumora pljuvačnih žlezda. Učestvuje sa oko 10-15% kod salivarnih neoplazmi. Karakteriše ga spor…
(more)
▼ Medicina / Medicine
Adenoidni cistični karcinom (ACC), je jedan od
najčešćih malignih tumora pljuvačnih žlezda. Učestvuje sa oko
10-15% kod salivarnih neoplazmi. Karakteriše ga spor rast, visoka
incidenca ka invaziji nerva, retko regionalno metastaziranje, česti
lokalni recidivi i spor razvoj progresivnih i relativno indolentnih
udaljenih metastaza. Najznačajniji faktori za prognozu ovog tumora
su klinički stadijum i histološki izgled tumora, prisustvo ili
odsustvo tumorskog tkiva na linijama resekcije, invazija nerva.
Apoptoza je regulatorni mehanizam tkivne homeostaze. Poremećaj
regulacije apoptoze je relativno česta karakteristika maligne
ćelije. U zavisnosti od stimulansa, putevi aktivacije apoptoze mogu
biti: receptorski (spoljašnji) i mitohondrijski (unutrašnji) put. U
unutrašnjem putu apoptoze značajnu ulogu igraju Apaf-1,
inicijatorne i efektorne kaspaze. Apaf-1 je multidomenski protein,
za koji se vezuje citohrom c i prokaspaza 9 što dovodi do
formiranja apoptozoma. Ovaj kompleks proteolitički aktivira
inicijatornu kaspazu-9. U završnoj fazi apoptoze, aktivirana
kaspaza 9 dovodi do aktivacije efektorne kaspaze 3, čijim ulaskom u
jedro započinje degradaciona faza apoptoze. Cilj U ovoj studiji
cilj je bio ispitati imunohistohemijsku ekspresiju Apaf-1, kaspaze
9 i 3 u ACC pljuvačnih žlezda, i rezultate korelirati sa
kliničko-patološkim parametrima kako bi se utvrdilo koji je od njih
nezavistan prognostički parametar. Materijal i metode Istraživanje
je koncipirano kao studija preseka, koja obuhvata 50 pacijenata sa
ACC pljuvačnih žlezda, muškog i ženskog pola, prosečne starosti 58
godina. U ovoj studiji koristili smo tehniku tkivnog mikroniza (TMA
kalupi). Preseci sa TMA kalupa, debljine 5μm, bojeni su
streptavidin-biotin imunohistohemijskom tehnikom pomoću primarnih
antitela specifičnih za: Apaf-1, kaspazu 9 i kaspazu 3. Obojeni
tkivni preseci su analizirani svetlosnim mikroskopom (Olympus tip
BH-2). Na osnovu prikupljenih podataka formirana je baza podataka u
–SPSS 18.0 koja je korišćena za dalju statističku obradu.
Statistička analiza podataka obuhvatala je metode deskriptivne i
analitičke (inferencijalne) statistike...
Advisors/Committee Members: Boričić, Ivan, 1956-.
Subjects/Keywords: adenoid cystic carcinoma; apoptosis; Apaf-1; caspase 9;
caspase 3.
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dožić, Branko S., 1. (2016). Imunohistohemijska analiza i prognostički značaj markera
unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu
pljuvačnih žlezda. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dožić, Branko S., 1968-. “Imunohistohemijska analiza i prognostički značaj markera
unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu
pljuvačnih žlezda.” 2016. Thesis, Univerzitet u Beogradu. Accessed April 22, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dožić, Branko S., 1968-. “Imunohistohemijska analiza i prognostički značaj markera
unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu
pljuvačnih žlezda.” 2016. Web. 22 Apr 2021.
Vancouver:
Dožić, Branko S. 1. Imunohistohemijska analiza i prognostički značaj markera
unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu
pljuvačnih žlezda. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Apr 22].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dožić, Branko S. 1. Imunohistohemijska analiza i prognostički značaj markera
unutrašnjeg puta apoptoze u adenoidnom cističnom karcinomu
pljuvačnih žlezda. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11642/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago
3.
Douglas, Hollian Raphaelle.
XIAP, a potential regulator of follicular atresia in the sheep ovary
.
Degree: 2011, University of Otago
URL: http://hdl.handle.net/10523/1635
► X-linked inhibitor of apoptosis (XIAP) is a strong inhibitor of initiator (-9) and executer (-3 and -7) caspase activity (Eckelman and Salvesen, 2006). In female…
(more)
▼ X-linked inhibitor of apoptosis (XIAP) is a strong inhibitor of initiator (-9) and executer (-
3 and -7)
caspase activity (Eckelman and Salvesen, 2006). In female reproductive research, XIAP has received minimal attention considering the ability to arrest caspases provides a potential mechanism for regulation of follicular atresia. This study aimed to test the hypotheses that XIAP is indicative of follicular health and regulates follicular atresia and to investigate the novel idea that prolactin (PRL) stimulates XIAP expression in the sheep ovary.
Estrous cycles of adult Romney ewes (N=31) were synchronized with Estrumate®. Tissue and blood samples were subsequently collected on either days 14, 15 and 16 or at twelve hour intervals during the follicular phase. Initially analysis involved developing a plasma PRL profile by radioimmunoassay. The presence and localization patterns of XIAP and prolactin receptor (PRLR) isoforms protein and/or mRNA were determined by RT-PCR, in situ hybridization histochemistry and immunohistochemistry. This was followed by a comparative study evaluating levels of active
caspase-
3 immunoreactivity and XIAP mRNA (N=22) or protein (N=23) expression in adjacent sections containing the same day 14, 15 and 16 antral follicles. Triple label immunofluorescence and confocal microscopy were subsequently used to further clarify XIAP and active
caspase-
3 protein localization and to establish the presence of an inverse expression relationship in granulosa and thecal cells. Differing doses of Ovine FSH and PRL, as potential stimuli of XIAP upregulation, were trialed in cultured granulosa cells. Finally, a provisional attempt to develop a granulosa cell death model for determination of PRL’s ability to upregulate XIAP was undertaken.
This study showed widespread XIAP expression during both luteal and follicular phases of the estrous cycle. XIAP protein was detected from the primary follicle stage onwards, whereas the mRNA was only evident in antral follicles, likely due to limited sensitivity of the in situ hybridization histochemical technique. This also proved problematic for detection of PRLR isoform mRNA, which was localized in luteal cells and three antral follicles only. In the comparative analysis, all day 14 antral follicles showed positive XIAP mRNA expression irrespective of active
caspase-
3 levels. Over 50% of the day 15 and 16 antral follicles scored, however, showed an inverse relationship between XIAP mRNA/protein and active
caspase-
3 protein levels in, as did XIAP protein in day 14 antral follicles. Interestingly, in healthy follicles XIAP expression in thecal tissue was widespread, becoming increasingly localized to interna layers as active
caspase-
3 immunopositive granulosa cells became prevalent, despite a lack of
caspase activity in the theca itself. Confocal microscopy showed active
caspase-
3 and XIAP colocalization in granulosa cells. No significant negative correlation in expression patterns was observed, possibly due to brief active
caspase-
3 expression at apoptosis onset…
Advisors/Committee Members: Hurst, Peter (advisor).
Subjects/Keywords: Ovary;
XIAP;
Active caspase-3;
Follicle
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Douglas, H. R. (2011). XIAP, a potential regulator of follicular atresia in the sheep ovary
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1635
Chicago Manual of Style (16th Edition):
Douglas, Hollian Raphaelle. “XIAP, a potential regulator of follicular atresia in the sheep ovary
.” 2011. Doctoral Dissertation, University of Otago. Accessed April 22, 2021.
http://hdl.handle.net/10523/1635.
MLA Handbook (7th Edition):
Douglas, Hollian Raphaelle. “XIAP, a potential regulator of follicular atresia in the sheep ovary
.” 2011. Web. 22 Apr 2021.
Vancouver:
Douglas HR. XIAP, a potential regulator of follicular atresia in the sheep ovary
. [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/10523/1635.
Council of Science Editors:
Douglas HR. XIAP, a potential regulator of follicular atresia in the sheep ovary
. [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1635

North Carolina State University
4.
Walters, Jad Anthony.
Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.
Degree: PhD, Biochemistry, 2009, North Carolina State University
URL: http://www.lib.ncsu.edu/resolver/1840.16/3363
► Effector procaspase-3 plays a vital role in carrying out the final steps of programmed cell death, leading to the destruction of the cell. Because dimerization…
(more)
▼ Effector procaspase-
3 plays a vital role in carrying out the final steps of programmed cell death, leading to the destruction of the cell. Because dimerization of (pro)
caspase-
3 is essential for enzyme stability and activity, it is important to understand the structural details of the interactions at the dimer interface.
We show a single site in the dimer interface of (pro)
caspase-
3 can be used to activate or inhibit the enzyme. The results presented here demonstrate activation of procaspase-
3, in the absence of intersubunit linker cleavage, which generates a constitutively active, uninhibitable enzyme that is very efficient in killing both healthy and diseased mammalian cells. Moreover, inhibition may also be achieved utilizing the same site and the structural studies reveal two novel pathways of inhibition. Overall, these studies show how the interactions at the dimer interface in procaspase-
3 are essential in formation of a competent active site.
In addition to the procaspase-
3 interface studies, several crystallographic studies are presented which are aimed at elucidating a structure of procaspase-
3. Finally, a comprehensive protocol for carrying out equilibrium folding studies and determining conformational stabilities of macromolecules is provided. All together, this work has lead to exciting and novel discoveries in the field of apoptosis, including new mechanisms to selectively manipulate cell death.
Advisors/Committee Members: A. Clay Clark, Committee Chair (advisor), Carl Hall, Committee Member (advisor), Paul Wollenzien, Committee Member (advisor), Bob Rose, Committee Member (advisor).
Subjects/Keywords: Activation; Inhibition; Dimer Interface; (Pro)caspase-3
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Walters, J. A. (2009). Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3363
Chicago Manual of Style (16th Edition):
Walters, Jad Anthony. “Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.” 2009. Doctoral Dissertation, North Carolina State University. Accessed April 22, 2021.
http://www.lib.ncsu.edu/resolver/1840.16/3363.
MLA Handbook (7th Edition):
Walters, Jad Anthony. “Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation.” 2009. Web. 22 Apr 2021.
Vancouver:
Walters JA. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. [Internet] [Doctoral dissertation]. North Carolina State University; 2009. [cited 2021 Apr 22].
Available from: http://www.lib.ncsu.edu/resolver/1840.16/3363.
Council of Science Editors:
Walters JA. Structure, Folding, and Assembly of (Pro)caspase-3. The Role of the Dimer Interface in Active Site Formation. [Doctoral Dissertation]. North Carolina State University; 2009. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3363
5.
Mariana Caroline Furian Pontin.
Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse.
Degree: 2018, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/
► O estresse causa modificações no epitélio intestinal, tais como o aumento de células caliciformes e da taxa de apoptose. O uso de alimentos nutracêuticos tem…
(more)
▼ O estresse causa modificações no epitélio intestinal, tais como o aumento de células caliciformes e da taxa de apoptose. O uso de alimentos nutracêuticos tem sido uma alternativa para amenizar essas modificações sobre o tecido epitelial. Desta forma, este trabalho teve como objetivo avaliar se a inclusão de colostro bovino, o qual é constituído de fatores antioxidantes, imunes e de crescimento, seria capaz de amenizar as consequências do estresse crônico sob o intestino. Para isso, juvenis de pacu (Piaractus mesopotamicus) adensados a 50 kg/m3 foram alimentados duas vezes ao dia até a saciedade com ração peletizada e semi-purificada sem (0%CBL) e com a inclusão de colostro bovino liofilizado em concentrações crescentes (10, 20 e 30%CBL), (n=4). Após 28 dias, foram coletados segmentos do intestino médio, S1 e S2, e reto. Os tecidos foram marcados com corantes histológicos para a quantificação de células caliciformes contendo mucinas neutras, ácidas
(incluindo sialo e sulfomucinas) e ácidas-neutras. Também foram mensurados o volume (Vv) e a densidade da superfície (Sv) da mucosa, por análise estereológica, e a espessura da camada muscular. A razão do número de cada tipo e subtipo de célula caliciforme sobre o Vv e Sv foi calculada para estimar a densidade de células caliciformes, Dv e Ds, respectivamente. A taxa apoptótica foi analisada qualitativamente através da intensidade (alta, média e baixa) da imunomarcação da caspase-3 nas células epiteliais. As dietas não influenciaram os parâmetros zootécnicos analisados (P>0,05). No reto, os grupos que receberam 20 e 30%CBL apresentaram menor número de células caliciformes contendo sulfomucinas e menor Ds em relação a 0 e 10% (P=0,0148 e 0,0198, respectivamente). No RT, Dv total e Dv de células caliciformes contendo mucinas ácidas foi maior em 0 e 30%CBL em relação a 20%CBL (P=0,0155 e 0,225, respectivamente). No S1, 10 e 30%CBL apresentaram maior Dv em relação a 20%CBL
(P=0,0540). A espessura da camada muscular, o Vv e a Sv não diferiram entre os tratamentos (P>0,05). No S2 e RT, a taxa de apoptose teve relação inversa à concentração de colostro bovino liofilizado adicionado na ração. Nos três segmentos, houve maior proporção de células caliciformes contendo mucinas ácidas do que neutras, sendo a maioria representada por sulfomucinas. Assim, a inclusão de colostro bovino liofilizado nas rações de juvenis de pacu adensados diminuiu a apoptose nos segmentos intestinais S2 e RT e também diminuiu o número de células caliciformes contendo sulfomucinas no RT, indicando que o colostro bovino liofilizado pode ser utilizado como alimento nutracêutico para pacus (Piaractus mesopotamicus) adensados, a fim de diminuir a taxa apoptótica e proteger o intestino contra enzimas bacterianas, uma das principais funções das sulfomucinas.
The stress causes changes in the intestinal epithelium, such as the increase in the number of goblet cells and on the
rate of apoptosis. The use of nutraceutical foods has been an alternative to soften these modifications on the epithelial tissue.…
Advisors/Committee Members: Débora Botequio Moretti, Jose Roberto Ferreira, Raul Machado Neto, Rafael Estevan Sabioni.
Subjects/Keywords: Adensamento; Caspase-3; Célula caliciforme; Teleósteo; Caspase-3; Goblet cell; Stocking density; Teleoste
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pontin, M. C. F. (2018). Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/
Chicago Manual of Style (16th Edition):
Pontin, Mariana Caroline Furian. “Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse.” 2018. Masters Thesis, University of São Paulo. Accessed April 22, 2021.
http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/.
MLA Handbook (7th Edition):
Pontin, Mariana Caroline Furian. “Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse.” 2018. Web. 22 Apr 2021.
Vancouver:
Pontin MCF. Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse. [Internet] [Masters thesis]. University of São Paulo; 2018. [cited 2021 Apr 22].
Available from: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/.
Council of Science Editors:
Pontin MCF. Proteção antioxidante do colostro bovino em células intestinais de juvenis de pacu (Piaractus mesopotamicus) submetidos a estresse. [Masters Thesis]. University of São Paulo; 2018. Available from: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-14082018-102616/
6.
Amptoulach, Sousana.
Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.
Degree: 2015, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)
URL: http://hdl.handle.net/10442/hedi/41730
► There is strong evidence that tumor growth is not only a result of uncontrolled cell proliferation but alsoof decreased apoptosis. Caspase-3 is a member of…
(more)
▼ There is strong evidence that tumor growth is not only a result of uncontrolled cell proliferation but alsoof decreased apoptosis. Caspase-3 is a member of interleukin- 1 beta-converting enzyme which is involved in theinduction of apoptosis. Data on the expression of caspase-3 in patients with gastric cancer and its association with patient outcome are somewhat contradictory. We aimed to investigate the potential relation of the expression of caspase-3 protein with response to therapy and overall survival in patients with advanced noncardia gastric cancer. Tumor tissue samples collected from 359 consecutive patients with gastric cancer stage IV were retrospectively analyzed for the expression of caspase-3 in the primary tumor. The DNA apoptotic index assessed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling method. All patients were followed up until death. Caspase-3 was expressed in 43.5 % of tumors. Caspase-3 expression compared to no expression was related with a higher DNA apoptotic index (p\0.05). In multivariate analysis, tumor expression of caspase-3 was found to be an independent predictor of poor treatment response and survival (p\0.05). Expression of caspase-3 in advanced gastric cancer is a predictor of poor response to treatment and survival. Further studies are needed to fully elucidate the prognostic value of caspase-3 expression in these patients.
Subjects/Keywords: Κασπάση-3; Απόπτωση; Γαστρικός καρκίνος; Caspase-3; Apoptosis; Gasric cancer
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Amptoulach, S. (2015). Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/41730
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Amptoulach, Sousana. “Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.” 2015. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 22, 2021.
http://hdl.handle.net/10442/hedi/41730.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Amptoulach, Sousana. “Μελέτη της απόπτωσης σε καρκίνωμα στομάχου.” 2015. Web. 22 Apr 2021.
Vancouver:
Amptoulach S. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/10442/hedi/41730.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Amptoulach S. Μελέτη της απόπτωσης σε καρκίνωμα στομάχου. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. Available from: http://hdl.handle.net/10442/hedi/41730
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas
7.
Carvalho, Virginia Campos, 1987-.
Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ .
Degree: Faculdade de Ciências Médicas; Programa de Pós-Graduação em Clínica Médica, 2019, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504
► Orientador: Ricardo de Lima Zollner
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2019-11-22T12:49:27Z (GMT). No. of…
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▼ Orientador: Ricardo de Lima Zollner
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2019-11-22T12:49:27Z (GMT). No. of bitstreams: 1 Carvalho_VirginiaDeCampos_M.pdf: 1050903 bytes, checksum: aaeb468aa719a6f984e07946417f4dc1 (MD5) Previous issue date: 2019
Resumo: Plantas medicinais apresentam historicamente um papel importante no desenvolvimento de medicamentos. Doenças inflamatórias e autoimunes são cada vez mais frequentes na população, como o diabetes mellitus tipo 1A (DM-1A), o que impulsiona a investigação do potencial terapêutico de compostos naturais. Modelos experimentais são utilizados para testar novos compostos com potencial efeito farmacológico e permitir melhor compreensão de seus mecanismos de ação. Os camundongos da linhagem NOD/ShiLtJ são utilizados, para estudos relacionados ao DM-1A por desenvolverem a doença espontaneamente, de maneira similar ao que
ocorre nos humanos. A Farmacopeia Brasileira descreve Passiflora sp (maracujá) como fitoterápico, estando relacionado ao tratamento de diversas doenças, o maracujá possui também ação hipoglicemiante, propriedade importante no controle do diabetes. Nosso grupo investigou a atividade antioxidante do extrato aquoso das folhas do maracujá doce (Passiflora alata Curtis) e sua ação em camundongos não obesos diabéticos (NOD), modelo experimental do DM-1A, O tratamento com extrato aquoso das folhas de Passiflora alata Curtis (EAFPA), diminuiu a incidência do diabetes, possivelmente por sua ação antiproliferativa e antioxidante. Tal efeito parece estar associado com a diminuição do infiltrado de células inflamatórias, como linfócitos T CD4+ e CD8+ nas ilhotas pancreáticas (insulite). Neste contexto, investigamos o efeito in vitro do extrato aquoso liofilizado da folha de EAFPA (100, 200 e 500 µg/mL) na indução de morte celular por apoptose em linfócitos T CD4+ e CD8+ provenientes de
camundongos NOD/ShiLtJ (não diabéticos). Para esta finalidade, avaliamos a frequência de células expressando caspases iniciadoras do processo apoptótico (caspase 9) e caspases efetoras (caspase-3). Adicionalmente avaliamos a liberação citoplasmática de citocromo C mitocondrial, um importante ativador da morte celular programada pela via intrínseca de apoptose. A análise das culturas tratadas na concentração de 200 ?g/mL do EAFPA, apresentou diminuição na expressão de caspase 9 ativa e maior viabilidade dos linfócitos. Por outro lado, nas analises de liberação de citocromo C, cerca de 80% das células analisadas não liberaram a proteina. Não foi observada necrose celular, indicando que outras vias de morte celular possam estar ativadas, levando-nos necessariamente a novas abordagens para caracterização destas vias
Medicinal plants historically have an important role in drug development. Inflammatory and autoimmune disease are increasing in the population, such as diabetes mellitus
type 1-A (DM-1A), that stimulates the investigation of the natural compounds therapeutic potential. Experimental models are…
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS, Zollner, Ricardo de Lima, 1954-, Pavin, Elizabeth João, Gonçalves, Gisele Mara Silva.
Subjects/Keywords: Passiflora alata; Diabetes Mellitus tipo 1A; Caspase 3; Caspase 9; Apoptose; Passiflora alata; Diabetes Mellitus, Type 1A; Caspase 3; Caspase 9; Apoptosis; Cytochromes c
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to Zotero / EndNote / Reference
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APA (6th Edition):
Carvalho, Virginia Campos, 1. (2019). Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ . (Masters Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504
Chicago Manual of Style (16th Edition):
Carvalho, Virginia Campos, 1987-. “Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ .” 2019. Masters Thesis, Universidade Estadual de Campinas. Accessed April 22, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504.
MLA Handbook (7th Edition):
Carvalho, Virginia Campos, 1987-. “Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ .” 2019. Web. 22 Apr 2021.
Vancouver:
Carvalho, Virginia Campos 1. Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ . [Internet] [Masters thesis]. Universidade Estadual de Campinas; 2019. [cited 2021 Apr 22].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504.
Council of Science Editors:
Carvalho, Virginia Campos 1. Efeito do extrato aquoso de Passiflora alata curtis na expressão de caspase 3, 9 e liberação citoplasmática de citocromo c mitocondrial em linfócitos t de camundongos Nod/ShiltJ . [Masters Thesis]. Universidade Estadual de Campinas; 2019. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335504
8.
川元, 康嗣.
TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.
Degree: 博士(医学), 2016, Nagasaki University / 長崎大学
URL: http://hdl.handle.net/10069/37166
► Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp…
(more)
▼ Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp infection. Lp infects both phagocytic and non-phagocytic cells such as airway epithelial cells; however, the role of TNF-α in airway epithelial cells is unknown. Methods: Human airway epithelial cell line NCI-H292 was infected with Lp NUL1 strain. After infection, both intracellular growth of Lp and cell death were evaluated after treating the cells with or without TNF-α. Apoptosis was examined by performing activated caspase-3/7 staining and by using a pan-caspase inhibitor. Results: Lp infected and replicated in NCI-H292 cells in a time-dependent manner, and TNF-α treatment of Lp-infected NCI-H292 cells inhibited Lp replication. Inhibitory effects of TNF-α on Lp replication were suppressed after treatment with a TNF-α-neutralizing antibody. Lp infection increased extracellular lactate dehydrogenase levels and decreased the number of living cells. Increased number of Lp-infected NCI-H292 cells showed caspase-3/7 activation, indicating they underwent apoptosis. TNF-α treatment inhibited Lp replication by increasing the apoptosis of NCI-H292 cells. Conclusions: Thus, our results suggested that airway epithelial cells were involved in the pathogenesis of Lp infection and that TNF-α played a protective role by inhibiting the intracellular replication of Lp and by increasing the apoptosis of Lp-infected airway epithelial cells. However, Lp infection should be investigated further in patients undergoing anti-TNF-α therapy who develop pneumonia.
Subjects/Keywords: Anti-TNF-α therapy; Cell death; Caspase 3; Caspase 7; Lung epithelial cells
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APA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
川元, . (2016). TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/37166
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
川元, 康嗣. “TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed April 22, 2021.
http://hdl.handle.net/10069/37166.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
川元, 康嗣. “TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する.” 2016. Web. 22 Apr 2021.
Vancouver:
川元 . TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/10069/37166.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
川元 . TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis : TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/37166
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia
9.
Krauss, Amy.
Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/29095
► Anthocyanidins induce apoptosis in some cancer cell lines, but studies describing the dose-dependent effects on cell populations are limited. My objective was to evaluate the…
(more)
▼ Anthocyanidins induce apoptosis in some cancer cell lines, but studies describing the dose-dependent effects on cell populations are limited. My objective was to evaluate the dose-dependent response of malvidin and delphinidin,
anthocyanidins with different chemical structures, on cell viability and apoptosis in HT-29 cells compared to curcumin, a known inducer of apoptosis. Lower concentrations of anthocyanidins increased cell viability by 12-16% compared to control. Higher
concentrations of ≥80 umol/L of curcumin, malvidin, and delphinidin decreased cell viability by 16-44%. Apoptosis was assessed by measuring caspase-3 and caspase-8 activities, as well as mitochondrial permeability. Only high concentrations (80 umol/L) of
anthocyanidins and curcumin increased caspase-3 and caspase-8 activity. Concentrations of 25, 50 and 80 umol/L malvidin and delphinidin significantly disrupted mitochondrial permeability, showing anthocyanidins may be more effective at inducing apoptosis
intrinsically. There was a significant interaction between anthocyanidin concentration and anthocyanidin type on cell viability, caspase-8 activity, and mitochondria permeability.
Subjects/Keywords: Anthocyanidins; Delphinidin; Malvidin; Curcumin; Apoptosis; Caspase-3; Caspase-8; HT-29 cells
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APA ·
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MLA ·
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CSE |
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to Zotero / EndNote / Reference
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APA (6th Edition):
Krauss, A. (2014). Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29095
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Krauss, Amy. “Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells.” 2014. Thesis, University of Georgia. Accessed April 22, 2021.
http://hdl.handle.net/10724/29095.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Krauss, Amy. “Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells.” 2014. Web. 22 Apr 2021.
Vancouver:
Krauss A. Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/10724/29095.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Krauss A. Malvidin and delphinidin exhibit a dose-dependent effect on cell viability and apoptosis in HT-29 cells. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29095
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Detraz, Morgane.
Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities.
Degree: Docteur es, Chimie, 2019, Normandie
URL: http://www.theses.fr/2019NORMR138
► La qualité du diagnostic oncologique, déterminant la thérapie et le pronostic du patient, repose sur différentes techniques d’imagerie ou modalités, associées à des molécules de…
(more)
▼ La qualité du diagnostic oncologique, déterminant la thérapie et le pronostic du patient, repose sur différentes techniques d’imagerie ou modalités, associées à des molécules de contraste afin de générer une image représentative de la situation tumorale et analysable par le praticien. L’imagerie moléculaire, incluant l’imagerie optique de fluorescence et l’imagerie nucléaire, est couramment utilisée pour la prise en charge oncologique des patients. Récemment, une classe hybride de molécules de contraste appelée MOMIA (MOnomolecular Multimodal Imaging Agent) a émergé, associant plusieurs modalités au sein d’une structure moléculaire unique. Cette combinaison performante cumule les forces de chacune des modalités combinées en palliant leur limitation respective. Dans ce cadre, ces travaux de thèse portent sur le développement et l’exploitation d’une plateforme polyvalente bimodale (fluorescente et radioactive) composée d’un synthon peptidique, radiomarquable par chélation du 99mTc, couplé à fluorophore présentant des propriétés spectroscopiques d’émission dans le proche infra-rouge, gamme spectrale privilégiée pour l’imagerie in vivo. Cette plateforme est finalement clickable à toute (bio)molécule d’intérêt, générant à façon des sondes bimodales en une étape de couplage. L’exploitation de cette plateforme bimodale clickable a été explorée dans le contexte du diagnostic oncologique via deux cibles enzymatiques principales : la caspase-3 et les tyrosine-kinases. Les étapes de conception, synthèse, optimisation, développement et validation biologique préliminaire in vitro de la plateforme et des sondes dérivées bimodales sont présentés
Oncologic healthcare and remission prognosis rely on a reliable and accurate diagnosis. Molecular imaging, including optical and nuclear imaging, is currently used for the management cancer therapy. Recently, a new class of bio-imaging agent called MOMIA (MOnomolecular Multimodal Imaging Agent) emerged by combining the synergistic strengths of several modalities on the same molecular structure. We envisioned to merge optical and nuclear imaging in order to develop a molecular tool offering a non-invasive, highly resolutive and sensitive detection. Our approach relies on a universal bimodal clickable scaffold with a selected targeting ligand. Two distinct enzymatic targets have been explored in the oncologic context: caspase-3 as a key component in an apoptotic program and tyrosine kinase inhibitors involved in lung cancer therapy. These multimodal sensors have a promising potential in translational clinical applications.
Advisors/Committee Members: Bohn, Pierre (thesis director), Renard, Pierre-Yves (thesis director).
Subjects/Keywords: Imagerie moléculaire; Multimodalité; Fluorescence; SPECT; Caspase-3; Erlotinib; Molecular imaging; Multimodality; Fluorescence; SPECT; Caspase-3; Erlotinib; 616.075
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Detraz, M. (2019). Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities. (Doctoral Dissertation). Normandie. Retrieved from http://www.theses.fr/2019NORMR138
Chicago Manual of Style (16th Edition):
Detraz, Morgane. “Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities.” 2019. Doctoral Dissertation, Normandie. Accessed April 22, 2021.
http://www.theses.fr/2019NORMR138.
MLA Handbook (7th Edition):
Detraz, Morgane. “Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities.” 2019. Web. 22 Apr 2021.
Vancouver:
Detraz M. Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities. [Internet] [Doctoral dissertation]. Normandie; 2019. [cited 2021 Apr 22].
Available from: http://www.theses.fr/2019NORMR138.
Council of Science Editors:
Detraz M. Développement d'outils bimodaux pour l'imagerie oncologique in vivo exploitant les modalités optique et nucléaire. : Development of bimodal in vivo oncologic imaging agent relying on optical and nuclear modalities. [Doctoral Dissertation]. Normandie; 2019. Available from: http://www.theses.fr/2019NORMR138
11.
Rodrigues, Bruna dos Santos.
Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3.
Degree: 2013, Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG)
URL: http://repositorio.bc.ufg.br/tede/handle/tede/3726
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Coordenação de Aperfeiçoamento de Pessoal de
Nível Superior - CAPES
The death mechanisms induced by a new synthetic compound (4-FTC) in adenocarcinoma prostate cells (PC-3) and its toxicity were investigated in this study. PC-3 cells cytotoxity was evaluated by MTT reduction assay. The mechanisms involved in PC-3 death and cell cycle were investigated by flow cytometry and colorimetric assays. The compound toxicity was analized by cytotoxicity of mononuclear cells (MTT reduction assay) and 3T3 cells (neutral red uptake assay), myelotoxicity, haemolytic activity and acute oral toxicity. 4-FTC has concentration dependent cytotoxic activity in PC-3 cells, and 184,6 μM IC50. Investigation of death mechanisms indicated death by apoptosis, because of the significant increase in phosphatidylserine externalization (109,83%), loss of mytochondrial membrane potential (41,96%), significant increase of DNA fragmentation (284,02%) and capases 3/7 and 9 activity increase, 13,12% and 12,8%, respectively. Furthermore, the treatment of PC-3
cells wih 4-FTC did not induce the reactive oxygen species production, as well as, the induction of acid autophagic vesicles generation and did not change the cell cycle significantly. Althought 4-FTC was able to modulate the expression of some proteins that regulate cell cycle, incresead the expression of p53, p21 and p27. Thus, the results suggests that 4-FTC induced PC-3 death by apoptosis dependent by mitochondrial pathway activation. In toxicity evaluation, 4-FTC presented 52,86 μM and 19,63 μM IC50 to mononuclear and 3T3 cells, respectively; 27,35 μM IC50 to hematopoietic precursors; low acute oral toxicity, classified in GHS category 5, and not significant haemolytic activity.
Neste trabalho, investigaram-se os mecanismos de morte induzidos por um novo composto sintético, 4-fluorbenzaldeidotiossemicarbazona (4-FTC), nas células de adenocarcinoma prostático PC-3 e alguns parâmetros da toxicidade desse composto. A citotoxicidade nas células PC-3 foi avaliada pelo método de
redução do MTT, método colorimétrico para avaliar a viabilidade celular. A investigação dos mecanismos de morte…
Advisors/Committee Members: Valadares, Marize Campos, Valadares, Marize Campos, Costa, Nádia do Lago, Cunha, Luiz Carlos da.
Subjects/Keywords: Tiossemicarbazona; Caspase; Apoptose; Célula PC-3; Thiosemicarbazone; Caspase; Apoptosis; PC-3 cells; CIENCIAS DA SAUDE::FARMACIA
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APA (6th Edition):
Rodrigues, B. d. S. (2013). Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3. (Masters Thesis). Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG). Retrieved from http://repositorio.bc.ufg.br/tede/handle/tede/3726
Chicago Manual of Style (16th Edition):
Rodrigues, Bruna dos Santos. “Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3.” 2013. Masters Thesis, Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG). Accessed April 22, 2021.
http://repositorio.bc.ufg.br/tede/handle/tede/3726.
MLA Handbook (7th Edition):
Rodrigues, Bruna dos Santos. “Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3.” 2013. Web. 22 Apr 2021.
Vancouver:
Rodrigues BdS. Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3. [Internet] [Masters thesis]. Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG); 2013. [cited 2021 Apr 22].
Available from: http://repositorio.bc.ufg.br/tede/handle/tede/3726.
Council of Science Editors:
Rodrigues BdS. Avaliação de toxicidade e potencial indutor de morte celular do 4-fluorbenzaldeidotiossemicarbazona contra células de adenocarcinoma de próstata PC-3. [Masters Thesis]. Universidade Federal de Goiás; Programa de Pós-graduação em Ciências Farmacêuticas (FF); UFG; Brasil; Faculdade Farmácia – FF (RG); 2013. Available from: http://repositorio.bc.ufg.br/tede/handle/tede/3726

NSYSU
12.
Liang, Wei-Zhe.
Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.
Degree: PhD, Biological Sciences, 2012, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617
► The effect of the natural essential oil carvacrol or the anesthetic propofol on cell viability, cell cycle distribution, reactive oxygen species (ROS), intracellular free Ca2+…
(more)
▼ The effect of the natural essential oil carvacrol or the anesthetic propofol on cell viability, cell cycle distribution, reactive oxygen species (ROS), intracellular free Ca2+ concentrations ([Ca2+]i) and
caspase-
3-associated apoptosis in human normal cells or non-normal cells is unclear. Human gingival fibroblasts (HGF), human oral cancer cell line (OC2) and human glioblastoma cell line (DRTBG-05MG, HGB) were used in this study. Cell viability was measured by detecting reagent water soluble tetrazolium salt-1 (WST-1). Apoptosis was detected by Annexin V/propidium iodide (PI) staining, cell cycle distribution was detected by PI staining, and ROS was detected by membrane-permeable probe dichlorofluorescein diacetate (DCFH-DA) or hydroethidine (HE) staining. Apoptosis, cell cycle distribution and ROS were analyzed by flow cytometry. The Ca2+-sensitive fluorescent dye fura-2 was applied to measure [Ca2+]i.
Caspase-
3 expression was detected by western blotting.
Carvacrol at 200-800 μM decreased the cell viability of OC2 or HGB cells in a concentration-dependent manner and 1,000 μM carvacrol almost killed all OC2 or HGB cells, but in HGF cells, 200-800 μM carvacrol did not significantly kill cells and 1,000 μM carvacrol decreased only about 63% of cell viability. Similarly, propofol at concentrations between 300 and 600 μM decreased the cell viability of OC2 or HGB cells in a concentration-dependent manner and 700 μM propofol almost killed all OC2 or HGB cells, but in HGF cells, 300-600 μM propofol did not significantly kill cells and 700 μM propofol decreased about 62% of cell viability. In OC2 or HGB cells, carvacrol (200 μM, 400 μM or 600 μM) or propofol (300 μM, 400 μM or 500 μM) induced apoptosis, increased ROS production, evoked cell cycle arrest and activated
caspase-
3. The
caspase-
3 inhibitor (DEVD-CHO) partially decreased the apoptotic effect induced by carvacrol or propofol.
On the other hand, in OC2 or HGB cells, carvacrol at concentrations between 400 μM and 1,000 μM induced a [Ca2+]i rise in a concentration-dependent manner and the signal was reduced by removal of extracellular Ca2+. In HGF cells, carvacrol at 1000 μM did not induce immediate [Ca2+]i rises in Ca2+-containing or Ca2+-free medium. Similarly, propofol at concentrations between 400 μM and 1,000 μM induced a [Ca2+]i rise in a concentration-dependent manner in OC2 or HGB cells, but not in HGF cells. This cytotoxic effect was not reversed in carvacrol-treated groups, but was partially reversed in propofol-treated groups when cytosolic Ca2+ was chelated with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl (BAPTA-AM) in OC2 or HGB cells. The apoptotic effect of propofol was also partially decreased by BAPTA-AM treatment in OC2 and HGB cells.
In OC2 and HGB cells, carvacrol- or propofol-induced Ca2+ signal was not altered by L-type voltage-gated Ca2+ channel blocker nifedipine, store-operated Ca2+ channel blocker econazole or SK&F96365) and protein kinase C (PKC) activator phorbol myristate acetate (PMA), but…
Advisors/Committee Members: Nai-Wen Tsai (chair), Chung-Ren Jan (chair), Ko-Long Lin (chair), Cheng-Hsien Lu (committee member), Yao-Dung Hsieh (chair).
Subjects/Keywords: Apoptosis; cell cycle; ROS; Ca2+; Caspase-3; Propofol; Carvacrol
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APA (6th Edition):
Liang, W. (2012). Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617
Chicago Manual of Style (16th Edition):
Liang, Wei-Zhe. “Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.” 2012. Doctoral Dissertation, NSYSU. Accessed April 22, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617.
MLA Handbook (7th Edition):
Liang, Wei-Zhe. “Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells.” 2012. Web. 22 Apr 2021.
Vancouver:
Liang W. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. [Internet] [Doctoral dissertation]. NSYSU; 2012. [cited 2021 Apr 22].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617.
Council of Science Editors:
Liang W. Effects of carvacrol and 2,6-diisopropylphenol (propofol) on reactive oxygen species (ROS)-, calcium (Ca2+)- and caspase-3-associated apoptosis in human normal cells and non-normal cells. [Doctoral Dissertation]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902112-105617

University of Miami
13.
Gillespie, Megan Marie.
Improving Sensing Through the Design of Recombinant Proteins.
Degree: PhD, Chemistry (Arts and Sciences), 2015, University of Miami
URL: https://scholarlyrepository.miami.edu/oa_dissertations/1460
► Genetic engineering has provided us with the ability to advance medicine and technology by creating proteins that can be used for various applications. Two such…
(more)
▼ Genetic engineering has provided us with the ability to advance medicine and technology by creating proteins that can be used for various applications. Two such applications are analyte detection and energy production. As more knowledge about protein structure and function becomes available and modeling programs are improved, researchers become better equipped to design and create mutant proteins to fit desired needs. In this research, we have genetically engineered proteins to create mutants that can be used for detection of analytes of interests and/or in biofuel cell development. The bioluminescent protein aequorin (AEQ) was mutated in a way that allows the protein to detect the protease
caspase-
3, a marker for apoptosis, by creating a measurable change in bioluminescence in the presence of the protease. We characterized the mutant and compared it to AEQ that had not been engineered to detect
caspase-
3. Then, we used this mutant AEQ to detect apoptosis both in vitro and in vivo. This dissertation also describes a mutated redox enzyme, glucose oxidase (GOx). Glucose oxidase has both the ability to detect glucose and to utilize glucose for energy at the anode of a biofuel cell. We engineered GOx to create a mutant that has the ability to transfer electrons more efficiently to an electrode, than the wild type enzyme. This was accomplished by mutating the amino acid that is the potential site of electron transfer from the enzyme's active center to oxygen. Finally, we expressed and purified two thermophilic proteins, phenol hydroxylase and laccase, that are both desired because of their stability and potential use at the cathode of biofuel cells.
Advisors/Committee Members: Leonidas Bachas, Roger Leblanc, James Wilson, Sapna Deo.
Subjects/Keywords: protein engineering; apoptosis; cornea; caspase-3; aequorin; glucose oxidase
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gillespie, M. M. (2015). Improving Sensing Through the Design of Recombinant Proteins. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/1460
Chicago Manual of Style (16th Edition):
Gillespie, Megan Marie. “Improving Sensing Through the Design of Recombinant Proteins.” 2015. Doctoral Dissertation, University of Miami. Accessed April 22, 2021.
https://scholarlyrepository.miami.edu/oa_dissertations/1460.
MLA Handbook (7th Edition):
Gillespie, Megan Marie. “Improving Sensing Through the Design of Recombinant Proteins.” 2015. Web. 22 Apr 2021.
Vancouver:
Gillespie MM. Improving Sensing Through the Design of Recombinant Proteins. [Internet] [Doctoral dissertation]. University of Miami; 2015. [cited 2021 Apr 22].
Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1460.
Council of Science Editors:
Gillespie MM. Improving Sensing Through the Design of Recombinant Proteins. [Doctoral Dissertation]. University of Miami; 2015. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/1460

University of Toronto
14.
Latif, Maya.
Regulation of Cisplatin-induced Programmed Cell Death In Vivo.
Degree: 2016, University of Toronto
URL: http://hdl.handle.net/1807/82403
► Cisplatin [ cis-PtCl2 NH3)2 ] is a widely utilized chemotherapeutic agent. Its proposed mechanism of action involves DNA-platinum adduct formation that triggers DNA damage responses,…
(more)
▼ Cisplatin [ cis-PtCl2 NH3)2 ] is a widely utilized chemotherapeutic agent. Its proposed mechanism of action involves DNA-platinum adduct formation that triggers DNA damage responses, cell cycle arrest and p53-mediated cell death. In vivo treatment of murine embryos with cisplatin caused widespread cellular injury consistent with induction of apoptosis. Caspase-3 knockout embryos exhibit complete inhibition of cell death 24 hours following cisplatin treatment in a variety of tissues. Examination at later time points revealed an alternate form of programmed cell death in the absence of caspase-3 exhibiting features of necroptosis, suggesting coordinated regulation of these disparate pathways is critical to control PCD signaling in vivo. These results demonstrate caspase-3 is necessary and sufficient to regulate cisplatin- mediated PCD in numerous embryonic tissues and could hold implications towards the maintenance of normal and malignant cells in a variety of solid tissues in the presence of cisplatin.
M.Sc.
Advisors/Committee Members: Henderson, Jeffrey T, Pharmaceutical Sciences.
Subjects/Keywords: Apoptosis; Caspase-3; Cisplatin; DNA damage; Necroptosis; PCD; 0307
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Latif, M. (2016). Regulation of Cisplatin-induced Programmed Cell Death In Vivo. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/82403
Chicago Manual of Style (16th Edition):
Latif, Maya. “Regulation of Cisplatin-induced Programmed Cell Death In Vivo.” 2016. Masters Thesis, University of Toronto. Accessed April 22, 2021.
http://hdl.handle.net/1807/82403.
MLA Handbook (7th Edition):
Latif, Maya. “Regulation of Cisplatin-induced Programmed Cell Death In Vivo.” 2016. Web. 22 Apr 2021.
Vancouver:
Latif M. Regulation of Cisplatin-induced Programmed Cell Death In Vivo. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/1807/82403.
Council of Science Editors:
Latif M. Regulation of Cisplatin-induced Programmed Cell Death In Vivo. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/82403

Laurentian University
15.
Albalawi, Ghadah.
The effect of andrographis paniculata on the growth of malignant cancer cells
.
Degree: 2016, Laurentian University
URL: https://zone.biblio.laurentian.ca/handle/10219/2674
► There are a variety of plants that have been recognized and used in traditional medicine for their health benefits. Among these plants is Andrographis paniculata,…
(more)
▼ There are a variety of plants that have been recognized and used in traditional medicine for their health benefits. Among these plants is Andrographis paniculata, commonly known as the king of bitters because of its bitter taste. In the past, Andrographis paniculata has been used to treat various ailments, including respiratory infections, the common cold, snake bites, inflammation and diarrhea. We have tested the effect of Andrographis paniculata extracts on the growth of malignant cancer cells and its potential benefits towards cancer treatment. Cancer cells, including B16- BL6, MCF-7, MDA-MB-231, 4T1, and ASPC-1 cells, were treated with 70% ethanol extracts or aqueous extracts of Andrographis paniculata with a range in concentration from 0.1 to 1%. Treatment with the ethanol extract of Andrographis paniculata inhibited cell growth when added at 0.25% to 1%. The aqueous extract was less potent and inhibited cell growth at only 1%. Treatment of B16- BL6 cells with 1% of the aqueous extract of Andrographis paniculata for 48 h induced apoptosis as detected using the acridine orange/ ethidium bromide cell staining assay. On the other hand, treatment with the ethanol extract had a much stronger effect and cell staining showed late stage apoptosis and loss of membrane integrity. We have shown that treatment of B16- BL6 cells with Andrographis paniculata extracts altered cell survival pathways and promoted apoptosis by using western blot analysis for ERK ½, phosphorylated-ERK and Caspase 3. Similarly, the treatment of B16-BL6 with the ethanol extract for 48 h increased the number of cells in the Sub-G1 phase, in comparison to treatment with the aqueous extract, by using flow cytometry of propidium iodide-stained cells. Overall, this study demonstrated that Andrographis paniculata had successfully suppressed the growth of malignant cancer cells.
Subjects/Keywords: Andrographis paniculata;
malignant cancer cells;
Apoptosis;
Caspase 3,;
Sub-G1
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Albalawi, G. (2016). The effect of andrographis paniculata on the growth of malignant cancer cells
. (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/handle/10219/2674
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells
.” 2016. Thesis, Laurentian University. Accessed April 22, 2021.
https://zone.biblio.laurentian.ca/handle/10219/2674.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Albalawi, Ghadah. “The effect of andrographis paniculata on the growth of malignant cancer cells
.” 2016. Web. 22 Apr 2021.
Vancouver:
Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells
. [Internet] [Thesis]. Laurentian University; 2016. [cited 2021 Apr 22].
Available from: https://zone.biblio.laurentian.ca/handle/10219/2674.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Albalawi G. The effect of andrographis paniculata on the growth of malignant cancer cells
. [Thesis]. Laurentian University; 2016. Available from: https://zone.biblio.laurentian.ca/handle/10219/2674
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University
16.
Harding, Charles P.
In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.
Degree: MS, Biological and Irrigation Engineering, 2019, Utah State University
URL: https://digitalcommons.usu.edu/etd/7436
► Muscle loss from lack of activity is a serious issue for immobilized patients on Earth and in human spaceflight, where the low gravity environment…
(more)
▼ Muscle loss from lack of activity is a serious issue for immobilized patients on Earth and in human spaceflight, where the low gravity environment prevents normal muscle activity. Simulating muscle loss in cultured cells is an important step in understanding how this condition occurs. This work evaluates different means of simulating muscle loss and selects the one that most closely mimics the cellular responses seen in animals and humans.
To simulate the microgravity environment of spaceflight, mouse skeletal muscle cells were grown in a rotary cell culture system (RCCS). Growing the cells within a natural gelled substrate was compared against growing them on the surface of small plastic beads. Changes after culture under simulated microgravity were characterized by assessing proteins and genes known to change during muscle loss. The structure of the cells was also evaluated by microscopy.
The mouse skeletal muscle cells grown on plastic beads in the RCCS had significant changes in multiple key genes associated with muscle loss and demonstrated physical characteristics expected of mature tissue in live animals. This model is a valuable platform for exploring muscle loss mechanisms and testing new drugs.
Advisors/Committee Members: Elizabeth Vargis, Timothy Taylor, Jon Takemoto, ;.
Subjects/Keywords: MuRF1; MAFbx; Caspase 3; C2C12; RCCS; Biological Engineering
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Harding, C. P. (2019). In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/7436
Chicago Manual of Style (16th Edition):
Harding, Charles P. “In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.” 2019. Masters Thesis, Utah State University. Accessed April 22, 2021.
https://digitalcommons.usu.edu/etd/7436.
MLA Handbook (7th Edition):
Harding, Charles P. “In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers.” 2019. Web. 22 Apr 2021.
Vancouver:
Harding CP. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. [Internet] [Masters thesis]. Utah State University; 2019. [cited 2021 Apr 22].
Available from: https://digitalcommons.usu.edu/etd/7436.
Council of Science Editors:
Harding CP. In Vitro Simulation of Microgravity Induced Muscle Loss Successfully Increases Expression of Key In Vivo Atrophy Markers. [Masters Thesis]. Utah State University; 2019. Available from: https://digitalcommons.usu.edu/etd/7436
17.
Ribeiro, Roana Cecília dos Santos.
Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia.
Degree: 2019, Universidade Estadual Paulista (UNESP)
URL: http://hdl.handle.net/11449/181062
► Submitted by Roana Cecília dos Santos Ribeiro ([email protected]) on 2019-03-18T14:30:20Z No. of bitstreams: 1 Dissertação Roana Ribeiro 2019.pdf: 3311637 bytes, checksum: 4d5e8b57c2b82d351fc8e0f885876b6f (MD5)
Rejected by…
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▼ Submitted by Roana Cecília dos Santos Ribeiro ([email protected]) on 2019-03-18T14:30:20Z No. of bitstreams: 1 Dissertação Roana Ribeiro 2019.pdf: 3311637 bytes, checksum: 4d5e8b57c2b82d351fc8e0f885876b6f (MD5)
Rejected by Tatiana Camila Gricio ([email protected]), reason: Solicitamos que realize a correção na submissão seguindo as orientações abaixo: 1 - Segundo a CAPES (Portaria nº 206, de 4 de setembro de 2018), o agradecimento deve conter exatamente a seguinte redação: O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Código de Financiamento 001. 2- Conforme orientado no tutorial, na submissão online é obrigatório adicionar também o resumo em inglês (abstract). Agradecemos a compreensão. on 2019-03-18T15:15:38Z (GMT)
Submitted by Roana Cecília dos Santos Ribeiro ([email protected]) on 2019-03-18T15:32:13Z No. of bitstreams: 1 Dissertação
Roana Ribeiro 2019.pdf: 3220627 bytes, checksum: 59658c515a3f47e40d16e90d1a3db7a4 (MD5)
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Made available in DSpace on 2019-03-18T15:43:48Z (GMT). No. of bitstreams: 1 ribeiro_rcs_me_jabo.pdf: 3220627 bytes, checksum: 59658c515a3f47e40d16e90d1a3db7a4 (MD5) Previous issue date: 2019-02-25
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O carcinoma de células escamosas (CCE) cutâneo é uma neoplasia maligna, que representa 15% dos tumores de pele em felinos. As regiões mais acometidas nos felinos pelo CCE são o plano nasal, pálpebras e pavilhões auriculares, tornando desafiadora a excisão cirúrgica com amplas margens. Desta forma, novas abordagens terapêuticas para o CCE cutâneo estão sendo investigadas, com destaque para a
eletroquimioterapia (EQT). A determinação das taxas de proliferação celular e apoptose tumoral por meio da imuno-histoquímica, permite avaliar o comportamento biológico tumoral, com otimização da evolução clínica do paciente. Este trabalho teve como objetivo analisar as expressões imuno-histoquímicas de Ki-67, COX-2 e caspase-3 e correlacioná-las com os aspectos clínico-patológicos e o tipo de resposta à EQT em CCE cutâneo de felinos; assim, determinar o potencial prognóstico e/ou preditivo destas variáveis. Para tanto, foram avaliadas 13 amostras de CCE cutâneo de felinos antes da EQT e as análises estatísticas quanto à intensidade de correlação entre as variáveis foram realizadas utilizando o coeficiente de correlação de Spearman, com nível de significância de 95%. Os resultados indicam que houve correlação positiva significativa entre o grau histopatológico e o grau de pleomorfismo celular (ρ=0,81, p<0,01), correlação negativa significativa entre o grau histopatológico e a
resposta à EQT (ρ= - 0,6; p=0,03), correlação positiva significativa entre o tamanho e o estadiamento tumoral…
Advisors/Committee Members: Universidade Estadual Paulista (UNESP), De Nardi, Andrigo Barboza [UNESP].
Subjects/Keywords: Caspase-3; COX-2; Ki-67; Valor preditivo; Valor prognóstico
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APA ·
Chicago ·
MLA ·
Vancouver ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ribeiro, R. C. d. S. (2019). Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia. (Masters Thesis). Universidade Estadual Paulista (UNESP). Retrieved from http://hdl.handle.net/11449/181062
Chicago Manual of Style (16th Edition):
Ribeiro, Roana Cecília dos Santos. “Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia.” 2019. Masters Thesis, Universidade Estadual Paulista (UNESP). Accessed April 22, 2021.
http://hdl.handle.net/11449/181062.
MLA Handbook (7th Edition):
Ribeiro, Roana Cecília dos Santos. “Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia.” 2019. Web. 22 Apr 2021.
Vancouver:
Ribeiro RCdS. Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia. [Internet] [Masters thesis]. Universidade Estadual Paulista (UNESP); 2019. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/11449/181062.
Council of Science Editors:
Ribeiro RCdS. Reatividade de marcadores de proliferação celular e de apoptose em carcinoma de células escamosas cutâneo de gatos como fatores preditivos à resposta ao tratamento com eletroquimioterapia. [Masters Thesis]. Universidade Estadual Paulista (UNESP); 2019. Available from: http://hdl.handle.net/11449/181062
18.
Leipelt, Juliano.
Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais.
Degree: 2016, Brazil
URL: http://hdl.handle.net/10737/1110&
► Submitted by FERNANDA DA SILVA VON PORSTER ([email protected]) on 2016-09-22T19:02:03Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) 2016JulianoLeipelt.pdf: 2508061 bytes, checksum: 100e43c73eddacc8441a11de473e260c…
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Made available in DSpace on 2016-09-29T19:20:08Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) 2016JulianoLeipelt.pdf: 2508061 bytes, checksum: 100e43c73eddacc8441a11de473e260c (MD5) Previous issue date: 2016-09
O câncer é apontado como a segunda maior causa de morte em todo o mundo, com previsão de em breve ser tornar a primeira.
O câncer de próstata está entre os 5 tipos de câncer mais diagnosticados em homens, sendo que o câncer hepático está em segundo lugar em taxa de mortalidade entre homens e mulheres. Indícios apontam para uma ativação das vias da inflamação associados a uma inibição das vias de morte celular no processo de carcinogênese. A regulação destas vias torna-se alvo importante e complementar no controle do câncer, sendo estimulada a busca de biomoléculas com este potencial. As plantas são importante fonte de descoberta de novas biomoléculas com ampla utilização para o tratamento de diversas patologias. A família Myrtaceae possui diversas espécies que são apontadas como fortes candidatos em potencial nesta busca, incluindo as do gênero Myrciaria. A espécie Myrciaria plinioides não possui estudos referentes suas propriedades terapêuticas ou a atuação em vias de sinalização envolvidas na inflamação ou na carcinogênese. Neste contexto, este estudo teve por objetivo avaliar a atividade do extrato
etanólico de M. plinioides em células de carcinoma hepatocelular (HepG2) e próstata (LNCaP) , através da análise de expressão dos marcadores p38-α, pp38-α, NF-κB e caspase-3, envolvidos na carcinogênese, e o efeito sobre a viabilidade celular através do método de MTT. A viabilidade das células foi alterada significativamente, em ambas as linhagens celulares quando tratadas com o extrato etanólico. A análise da expressão proteica demonstra significativa inibição da expressão de p38-α e caspase-3 nas células LNCaP, quando tratadas com extrato etanólico de M. plinioides seguido de LPS. Em células HepG2, somente houve alteração na expressão da caspase-3 na concentração de 200 μg/mL, com ou sem adição de LPS após tratamento com extrato. Os resultados deste estudo demonstraram redução da viabilidade celular nas duas linhagens tumorais, expressão diferenciada de proteínas envolvidas em apoptose, o que leva a indícios da ativação de mecanismos distintos pelo extrato em cada tipo celular.
Estudos futuros para averiguar o mecanismo celular e a indução de morte em células tumorais de câncer de próstata e…
Subjects/Keywords: LNCaP; HepG2; p38α; pp38α; NF-Kb; Caspase-3
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Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Leipelt, J. (2016). Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais. (Masters Thesis). Brazil. Retrieved from http://hdl.handle.net/10737/1110&
Chicago Manual of Style (16th Edition):
Leipelt, Juliano. “Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais.” 2016. Masters Thesis, Brazil. Accessed April 22, 2021.
http://hdl.handle.net/10737/1110&.
MLA Handbook (7th Edition):
Leipelt, Juliano. “Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais.” 2016. Web. 22 Apr 2021.
Vancouver:
Leipelt J. Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais. [Internet] [Masters thesis]. Brazil; 2016. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/10737/1110&.
Council of Science Editors:
Leipelt J. Avaliação in vitro do potencial biológico de Myrciaria plinioides (D. Legrand) em células tumorais. [Masters Thesis]. Brazil; 2016. Available from: http://hdl.handle.net/10737/1110&

Queensland University of Technology
19.
Burgess, Joshua T.
The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.
Degree: 2016, Queensland University of Technology
URL: http://eprints.qut.edu.au/98661/
► SASH1 (SAM and SH3 domain-containing protein 1) is a putative tumour suppressor functioning in the control of apoptosis and cellular proliferation. In summary this project…
(more)
▼ SASH1 (SAM and SH3 domain-containing protein 1) is a putative tumour suppressor functioning in the control of apoptosis and cellular proliferation. In summary this project has further characterised the role of SASH1 in genome maintenance, including DNA repair and apoptosis, which has implications on its links with tumourigenesis. In addition we have also identified that targeting SASH1 may be a novel cancer treatment target and shown that Chloropyramine may act as an effective anti-cancer agent through a SASH1-dependent pathway.
Subjects/Keywords: SASH1; Caspase 3; Chloropyramine; NF-κ; B; DNA Damage repair; hSSB1; Apoptosis; Breast Cancer; Lung Cancer; 14-3-3
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Burgess, J. T. (2016). The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/98661/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Burgess, Joshua T. “The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.” 2016. Thesis, Queensland University of Technology. Accessed April 22, 2021.
http://eprints.qut.edu.au/98661/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Burgess, Joshua T. “The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis.” 2016. Web. 22 Apr 2021.
Vancouver:
Burgess JT. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. [Internet] [Thesis]. Queensland University of Technology; 2016. [cited 2021 Apr 22].
Available from: http://eprints.qut.edu.au/98661/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Burgess JT. The role of SAM and SH3 domain-containing protein 1 (SASH1) in DNA damage repair and tumourigenesis. [Thesis]. Queensland University of Technology; 2016. Available from: http://eprints.qut.edu.au/98661/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Ol, Kevser Kuşat.
Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması
.
Degree: ESOGÜ, Tıp Fakültesi, Tıbbi Biyokimya, 2014, Eskisehir Osmangazi University
URL: http://hdl.handle.net/11684/892
► Bu çalışmada modifiye sıvı diyet aracılığıyla prenatal ve postnatal etanol maruziyeti oluşturulan rat yavrularının serebral korteksindeki nörodejenerasyonun etkisini incelemeyi ve betain ve omega-3 takviyesinin koruyucu…
(more)
▼ Bu çalışmada modifiye sıvı diyet aracılığıyla prenatal ve postnatal etanol
maruziyeti oluşturulan rat yavrularının serebral korteksindeki nörodejenerasyonun
etkisini incelemeyi ve betain ve omega-
3 takviyesinin koruyucu etkilerini göstermeyi
amaçladık. Prenatal ve postnatal periyodda etanole maruz kalındığında yavrularda Fetal
Alkol Sendromu (FAS) görülmektedir. Ratlar kontrol, etanol, etanol+omega-
3,
etanol+betain, etanol+betain+omega-
3 şeklinde 5 gruba ayrıldı. Rat yavrularının
serebral korteksindeki etanol indüksiyonuna bağlı değişiklerde betain ve omega-
3 ün
etkilerini incelemek amacıyla biyokimyasal olarak sitokrom c, kaspaz-
3, kalpain,
katepsin B ve L, DNA fragmantasyonu seviyeleri incelenmiş histolojik ve morfometrik
bulgular elde edilmiştir.
Etanol grubunda kaspaz-
3, kalpain, katepsin B, sitokrom c seviyeleri kontrol
grubu ile karşılaştırıldığında belirgin derecede artmıştır. Dahası, kaspaz-
3 ve kalpain
seviyeleri etanol+omega-
3, etanol+betain, etanol+betain+omega-
3 gruplarında etanol
grubuyla karşılaştırıldığında azalmıştır. Aynı zamanda katepsin B düzeyleri
etanol+betain grubunda kontrolden daha yüksektir. Etanol+betain+omega-
3 grubunda
katepsin B etanol ve etanol+betain gruplarıyla karşılaştırıldığında azalmıştır. Katepsin L
ve DNA fragmantasyonunda istatistiksel olarak farklılık bulunamamıştır. Doku hasarı
değerlendirmesi histolojik açıdan; kontrol, etanol+omega-
3, etanol+betain,
etanol+betain+omega-
3 gruplarında hemoraji, PMNL, mikroglia etanol grubuyla
karşılaştırıldığında istatistiksel olarak düşüktür. Aynı zamanda etanol+omega-
3 ve
kontrol gruplarında konjesyon ve nekroz etanol indüklü hasara göre azalış göstermiştir.
Morfometrik analiz sonucu etanol alınımı etanol+omega-
3 ve kontrol gruplarına göre
nekrotik hücre sayısını artırmıştır.
Sonuç olarak, postnatal etanol maruziyeti rat beyinlerinde nekrotik hücre ölümünü
tetiklemektedir, omega-
3 ve betain nörodejenerasyonu azaltmaktadır. Omega-
3 ve
betain özellikle FAS ın önlenmesinde, nörodejenerasyon açısından yararlı olabilir.
Advisors/Committee Members: Kanbak, Güngör (advisor).
Subjects/Keywords: Betain;
Fetal Alkol Sendromu (FAS);
Kaspaz-3;
Omega-3;
Sitokrom c;
Caspase-3;
Betaine;
Cytochrome c;
Fetal Alcohol Syndrome (FAS)
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ol, K. K. (2014). Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması
. (Thesis). Eskisehir Osmangazi University. Retrieved from http://hdl.handle.net/11684/892
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ol, Kevser Kuşat. “Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması
.” 2014. Thesis, Eskisehir Osmangazi University. Accessed April 22, 2021.
http://hdl.handle.net/11684/892.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ol, Kevser Kuşat. “Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması
.” 2014. Web. 22 Apr 2021.
Vancouver:
Ol KK. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması
. [Internet] [Thesis]. Eskisehir Osmangazi University; 2014. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/11684/892.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ol KK. Prenatal ve postnatal alkol kullanımı sonucunda rat beyinlerinde oluşan nörodejenerasyonun betain ve/veya omega-3 uygulanmasıyla önlenmesinin araştırılması
. [Thesis]. Eskisehir Osmangazi University; 2014. Available from: http://hdl.handle.net/11684/892
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Johannes Gutenberg Universität Mainz
21.
Golbs, Antje.
Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.
Degree: 2008, Johannes Gutenberg Universität Mainz
URL: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/
► Die Apoptose spielt eine entscheidende Rolle während der normalen Entwicklung des zentralen Nervensystems. Elektrische Aktivität und die Versorgung mit trophischen Faktoren sind ausschlaggebend für das…
(more)
▼ Die Apoptose spielt eine entscheidende Rolle während der normalen Entwicklung des zentralen Nervensystems. Elektrische Aktivität und die Versorgung mit trophischen Faktoren sind ausschlaggebend für das Überleben von Neuronen. Um zu untersuchen, welche zellulären Prozesse die aktivitätsabhängige Apoptose in organotypischen Schnittkulturen des neugeborenen Neokortex beeinflussen, wurde in der vorliegenden Arbeit immunzytochemisch das Auftreten aktivierter Caspase-3, nach pharmakologischer Beeinflussung von Ionenkanälen und membranständigen Rezeptoren analysiert. Die Unterdrückung neuronaler Aktivität durch den Natriumionenkanalblocker TTX führte zu einem signifikanten Verlust kortikaler Neuronen. Ein ähnlicher Anstieg der Zahl apoptotischer Neurone konnte durch Applikation von Antagonisten ionotroper Glutamatrezeptoren, GABAA-Rezeptoren oder neuronaler Gap Junctions induziert werden. Jedoch konnte bei einigen Antagonisten die apoptosefördernde Wirkung erst nach längerer Einwirkung beobachtet werden. Im Weiteren wurde eine Methode etabliert, mit deren Hilfe eine Echtzeitanalyse der Apoptose kortikaler Neurone unter dem Entzug trophischer Faktoren in Gegenwart unterschiedlicher extrazellulärer Kaliumkonzentrationen ermöglicht wurde. Dazu wurden dissoziierte kortikale Kulturen mit dem pCaspase3-sensor Vektor transfiziert. Das durch dieses Plasmid codierte fluoreszente Protein wird Caspase-3 abhängig gespalten. In der vorliegenden Arbeit konnte gezeigt werden, dass der Caspase3-sensor spezifisch für die Aktivierung der Caspase-3 ist, und dass die Überlebensfähigkeit der transfizierten Neurone durch das Transfektionsprotokoll nicht beeinflusst wird.
Apoptosis is an essential process during normal development of the brain. Electrical activity and supply with survival factors play a major role in regulating neuronal survival. I aimed at correlating neuronal activity and apoptosis in the developing murine neocortex. Therefore organotypic slice cultures of newborn mouse cerebral cortex were treated with antagonists of different ligand-gated receptors and voltage-dependent ion channels. Apoptosis was assessed using an antibody specific for the activated form of caspase-3. Blocking network activity by the sodium channel blocker TTX caused a significant loss of cortical neurons. Moreover application of antagonists of ionotropic glutamate receptors, GABAA receptors or neuronal gap junctions resulted in a similar increase in the number of apoptotic neurons. However some antagonists increased neuronal loss only after prolonged exposure. In a further approach an assay was established which involves the transfection of dissociated cultures of the newborn mouse neocortex with the pCaspase3-sensor vector encoding a protein sensitive to cleavage by caspase-3. With this assay the impact of different extracellular potassium concentrations on caspase-3 dependent cortical apoptosis following trophic deprivation should be elucidated. The method was found to be specific for caspase 3 activation and did not interfere with the viability of…
Subjects/Keywords: Apoptose, elektrische Aktivität, Caspase-3, Neuronen, neuronale Entwicklung, Neokortex, Neurotrophine, Calcium, Kalium; apoptosis, electrical activity, caspase-3, neuron, neuronal development, neocortex, neurotrophins, calcium, potassium; Natural sciences and mathematics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Golbs, A. (2008). Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/
Chicago Manual of Style (16th Edition):
Golbs, Antje. “Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.” 2008. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed April 22, 2021.
http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/.
MLA Handbook (7th Edition):
Golbs, Antje. “Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung.” 2008. Web. 22 Apr 2021.
Vancouver:
Golbs A. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2008. [cited 2021 Apr 22].
Available from: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/.
Council of Science Editors:
Golbs A. Aktivitätsabhängige Kontrolle der Apoptose in neonatalen kortikalen Neuronen und Etablierung eines Biosensors zur Echtzeitanalyse der Caspase-3-Aktivierung. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2008. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2008/1617/
22.
Raquel Autran Coelho.
Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.
Degree: 2008, Universidade Federal de São Paulo
URL: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563
► Objetivos: Estudar a expressao imuno-histoquimica de topoisomerase II e de caspase-3 ativada, marcadores de proliferacao e de apoptose, respectivamente, a deteccao de DNA HPV e…
(more)
▼ Objetivos: Estudar a expressao imuno-histoquimica de topoisomerase II e de caspase-3 ativada, marcadores de proliferacao e de apoptose, respectivamente, a deteccao de DNA HPV e a evolucao da lesao cervical em mulheres portadoras de lesao intra-epitelial escamosa de baixo grau (LBG). Metodos: Foram avaliadas 40 mulheres portadoras de LBG e 32 sem neoplasia cervical, diagnosticadas por exame cito-colpo-histopatologico, quanto a imunoexpressao de topoisomerase II e de caspase-3 ativada e quanto a deteccao de DNA HPV por PCR consensual (GP5+/GP6+) em material de esfregaco cervico-vaginal. Os achados foram relacionados as variaveis clinicas das pacientes e a evolucao clinica das lesoes cervicais em 12 meses. As pacientes assinaram termo de consentimento livre e esclarecido. Resultados: A media percentual de celulas imunomarcadas por topoisomerase foi de 11,71% e 4,13%, no grupo com LBG e controle, respectivamente, com diferenca estatisticamente significante. Observou-se que houve expressao de caspase-3 em 17 (42,5%) e em 5 (15,63%) pacientes com e sem LBG, respectivamente, com diferenca estatisticamente significante. Foi detectado HPV DNA em 65% das pacientes com LBG e em 59,4% das pacientes sem lesao cervical, sem relacao com a expressao de topoisomerase II ou caspase-3. Na presenca de DNA-HPV, a expressao de topoisomerase II no grupo com LBG foi significativamente maior do que em fragmentos sem lesao. Nao foi observada diferenca quanto a evolucao da lesao cervical em 12 meses de acordo com a imunoexpressao de topoisomerase II. Com relacao a caspase-3 ativada, a maioria das pacientes com imuno-histoquimica negativa teve regressao da lesao cervical. Conclusoes: A imunoexpressao de topoisomerase II e de caspase-3 ativada podem ser considerados marcadores de proliferacao e de apoptose em lesao cervical de baixo grau, sem relacao com a presenca de DNA-HPV.
Purpose: To evaluate the correlation between the expression of topoisomerase II alpha, active caspase-3 and infection with human papillomavirus in low-grade cervical intraepithelial lesion and in the normal cervix, and whether they might influence susceptibility to, or evolution of, cervical lesion. Patients and methods: Forty cervical biopsies patients with low-grade cervical intraepithelial lesion and thirty-two with normal cervix were stained by immunohistochemistry for topoisomerase IIá and active caspase-3 and were investigated for the presence of HPV on exfoliated cells by general primer GP5+/6+ PCR amplification of DNA. These findings were correlated with clinicopathological features of the patients including their clinical outcome after twelve months. Subjects provided written informed consent. Results: Low-grade CIN patients as a group had a significantly higher expression of topoisomerase II alpha compared to controls, without correlation to disease outcome at 12 months. Caspase-3 was expressed in 42.5% of CIN patients and in 15.63% without disease, and most of women without caspase-3 receded cervical lesion. HPV DNA testing was positive in 65% of the patients…
Advisors/Committee Members: Ismael Dale Cotrim Guerreiro da Silva, Neila Maria de Góis Speck, Gustavo Rubino de Azevedo Focchi, Julisa Chamorro Lascasas Ribalta, José Eleutério Junior, Roberto Zamith.
Subjects/Keywords: DNA topoisomerases tipo II; Caspase 3; Neoplasia intraepitelial cervical; Infecções por papilomavírus; GINECOLOGIA E OBSTETRICIA; DNA topoisomerases type II; Caspase 3; Cervical intraepithelial neoplasia; Papillomavirus infections
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Coelho, R. A. (2008). Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. (Thesis). Universidade Federal de São Paulo. Retrieved from http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Coelho, Raquel Autran. “Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.” 2008. Thesis, Universidade Federal de São Paulo. Accessed April 22, 2021.
http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Coelho, Raquel Autran. “Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau.” 2008. Web. 22 Apr 2021.
Vancouver:
Coelho RA. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. [Internet] [Thesis]. Universidade Federal de São Paulo; 2008. [cited 2021 Apr 22].
Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Coelho RA. Expressao de topoisomerase II alfa e de caspase-3 ativada em lesao intra-epitelial cervical escamosa de baixo grau. [Thesis]. Universidade Federal de São Paulo; 2008. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=2563
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Champagne, Julien.
Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.
Degree: Docteur es, Biologie Santé, 2018, Montpellier
URL: http://www.theses.fr/2018MONTT018
► Chez la femme, le cancer du sein est le cancer le plus fréquemment diagnostiqué. Différents traitements sont disponibles selon le sous-type tumoral. Cependant, certaines patientes…
(more)
▼ Chez la femme, le cancer du sein est le cancer le plus fréquemment diagnostiqué. Différents traitements sont disponibles selon le sous-type tumoral. Cependant, certaines patientes sont réfractaires à ces thérapies et restent vulnérables lors de récidives. Le cancer a longtemps été défini par une division aberrante des cellules, mais aujourd'hui, il est évident que la résistance à la mort cellulaire programmée est un paramètre majeur dans l'étiologie de la maladie.Les cyclines de type D régulent le cycle cellulaire en permettant la transition de la phase G1 à la phase S. Pour cela, elles activent les kinases dépendantes des cyclines 4/6 (CDK4/6) qui phosphorylent les protéines du rétinoblastome ce qui libère le facteur de transcription E2F. La Cycline D1 (CycD1) nucléaire est donc centrale dans le contrôle du cycle. Son gène est amplifié dans les cancers humains et la moitié des patientes atteintes d'un cancer du sein ont une surexpression de CycD1. Par l’activation de CDK4, CycD1 est essentielle à l'apparition et à la progression tumorale. Ainsi, des inhibiteurs spécifiques de CDK4/6 ont été développés contre le cancer du sein. Malheureusement, certaines patientes restent insensibles à ce traitement. À ce titre, le ciblage spécifique de CycD1 pourrait représenter une alternative clinique. En effet, en plus de la régulation du cycle, CycD1 est également impliquée, indépendamment de CDK4, dans la survie des cellules cancéreuses. Cependant, aucun mécanisme de l'impact de CycD1 dans le maintien tumoral n'a été établi pour démontrer ce potentiel thérapeutique. En outre, CycD1 a été décrite dans les organes à l’âge adulte pour réguler le métabolisme du glucose et l'hématopoïèse. Par conséquent, pour éviter tout effet secondaire indésirable, nous avons décidé d’évaluer l’implication potentielle de CycD1 dans les organes adultes. Grâce au Tandem-HTRF, basé sur le transfert d'énergie entre deux anticorps, nous avons révélé la dynamique inattendue de CycD1 dans chaque organe adulte. De plus, nous avons montré que l’altération de l'expression de CycD1 conduit à une diminution des capacités de survie des cellules saines post-mitotiques.Au vu de ces limitations, nous avons développé une nouvelle approche d'ARN interférence spécifique des cellules cancéreuses appelée TAG-RNAi. Cette technologie permet de cibler CycD1 uniquement dans la tumeur afin d'épargner les cellules saines. Cette approche innovante consiste à cibler un tag présent uniquement sur l’ARNm de CycD1 des cellules cancéreuses. Ainsi, nous avons découvert que le ciblage spécifique de CycD1 induit une régression rapide et spontanée des tumeurs dépendantes des oncogènes RAS ou ERBB2. Par protéomique in vivo, j'ai découvert que lors de stress pro-apoptotiques, CycD1 cytoplasmique interagit avec la procaspase-
3 et bloque son activation pour empêcher l'apoptose des cellules. Ces travaux démontrent la valeur clinique du ciblage spécifique de CycD1 dans les cancers afin d'améliorer l'efficacité des chimiothérapies.Par conséquent, il restait à déterminer comment appliquer le…
Advisors/Committee Members: Pin, Jean-Philippe (thesis director).
Subjects/Keywords: Cancer du sein; Cycline D1; Apoptose; Caspase-3; Thérapie à la carte; ARN interférence; Breast cancer; Cyclin D1; Apoptosis; Caspase-3; Personalized medecine; RNA interference
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Champagne, J. (2018). Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2018MONTT018
Chicago Manual of Style (16th Edition):
Champagne, Julien. “Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.” 2018. Doctoral Dissertation, Montpellier. Accessed April 22, 2021.
http://www.theses.fr/2018MONTT018.
MLA Handbook (7th Edition):
Champagne, Julien. “Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival.” 2018. Web. 22 Apr 2021.
Vancouver:
Champagne J. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. [Internet] [Doctoral dissertation]. Montpellier; 2018. [cited 2021 Apr 22].
Available from: http://www.theses.fr/2018MONTT018.
Council of Science Editors:
Champagne J. Etude du rôle de la cycline D1 dans la survie cellulaire : Cyclin D1 involvment in cell survival. [Doctoral Dissertation]. Montpellier; 2018. Available from: http://www.theses.fr/2018MONTT018
24.
Maria Zilah Benetone.
Apoptose e proliferação na placenta de búfalas.
Degree: 2005, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/
► A apoptose é um processo fisiológico que desempenha papel crucial no desenvolvimento, remodelagem e senescência teciduais, inclusive placentários. A placenta, enquanto órgão temporário, atravessa todas…
(more)
▼ A apoptose é um processo fisiológico que desempenha papel crucial no desenvolvimento, remodelagem e senescência teciduais, inclusive placentários. A placenta, enquanto órgão temporário, atravessa todas estas fases em aproximadamente 10 meses, na espécie bubalina. O crescimento da placenta e a nutrição fetal requerem altas taxas de renovação e diferenciação celulares, e a maturação placentária está relacionada à redução das células epiteliais das criptas carunculares maternas. As modificações morfológicas celulares decorrentes do processo de apoptose são fruto de eventos bioquímicos complexos promovidos por uma família de cisteína-proteases, as caspases, especialmente as caspases executoras, dentre as quais se destaca a
caspase-
3, capaz de degradar várias proteínas citoplasmáticas e nucleares. Durante a apoptose, ocorre a clivagem
caspase-mediada da citoqueratina 18, proteína dos filamentos intermediários do citoesqueleto, e com isso a formação de
um neoepítopo específico. Por meio de métodos imunoistoquímicos pode-se detectar a presença tanto deste neoepítopo, quanto da forma ativa da
caspase-
3, o que demonstra que a célula entrou em estágio irreversível de morte celular. Morfologicamente, algumas das principais alterações celulares observadas são condensação da cromatina, a degradação e fragmentação do DNA, a formação de ?blebs? (pregas/bolhas) na membrana plasmática, além da fragmentação celular em corpúsculos apoptóticos, os quais podem ser identificados em cortes corados pelo método de rotina hematoxilina e eosina, utilizando-se microscópio de imunofluorescência, devido à eosinofluorescência das células em apoptose. Assim como a apoptose, a proliferação celular participa no equilíbrio homeostático tissular. Neste estudo, pretende-se avaliar a ocorrência de apoptose e proliferação celular em 42 placentônios de diferentes animais em diversas fases gestacionais (2-10 meses de gestação), em tecidos fixados em 4%
paraformoldeído, incluídos em paraplast e submetidos à imunoistoquímica (anticorpo monoclonal M30 CytoDeath;
Caspase-
3 Clivada; PCNA - antígeno de marcação nuclear), sendo também avaliada a presença de corpúsculos apoptóticos eosinofluorescentes nas amostras. Utilizando-se M30 e
caspase-
3 clivada pudemos constatar a ocorrência de apoptose nos epitélios uterino e trofoblástico, em células gigantes placentárias e, ocasionalmente, em células mesenquimais fetais, do estroma uterino e endoteliais. Não houve diferenças significativas (p<0.05) entre os métodos adotados, mas sim entre os estágios gestacionais. Para o M30, houve um aumento significante da apoptose do primeiro grupo (2-4.5 meses) em relação ao quarto grupo (9-10 meses); no caso da
Caspase-
3 Clivada houve um aumento estatísticamente significante (p<0.05) entre os três primeiros grupos (2-4.5; 5-6.5; e 7-8.5 meses de gestação, respectivamente) de animais em relação ao quarto grupo. Para o PCNA, ocorreu uma
diminuição no número de células em proliferação dos dois primeiros grupos de animais em relação ao quarto grupo (p<0.05). A presença de corpúsculos…
Advisors/Committee Members: Maria Angélica Miglino, Hiro Goto, José Luiz Guerra.
Subjects/Keywords: Apoptose; Búfalos; Caspase-3; Placenta; Proliferação; Apoptosis; Buffalo; Caspase 3; Placenta; Proliferation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Benetone, M. Z. (2005). Apoptose e proliferação na placenta de búfalas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/
Chicago Manual of Style (16th Edition):
Benetone, Maria Zilah. “Apoptose e proliferação na placenta de búfalas.” 2005. Masters Thesis, University of São Paulo. Accessed April 22, 2021.
http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/.
MLA Handbook (7th Edition):
Benetone, Maria Zilah. “Apoptose e proliferação na placenta de búfalas.” 2005. Web. 22 Apr 2021.
Vancouver:
Benetone MZ. Apoptose e proliferação na placenta de búfalas. [Internet] [Masters thesis]. University of São Paulo; 2005. [cited 2021 Apr 22].
Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/.
Council of Science Editors:
Benetone MZ. Apoptose e proliferação na placenta de búfalas. [Masters Thesis]. University of São Paulo; 2005. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-23062006-182309/

NSYSU
25.
Chou, Chiang-Ting.
Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline.
Degree: PhD, Biological Sciences, 2008, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559
► Depression is a physiological disorder that may be treated by increasing the bodyâs amount of one or a few of the following neurotransmitters: serotonin, dopamine…
(more)
▼ Depression is a physiological disorder that may be treated by increasing the bodyâs amount of one or a few of the following neurotransmitters: serotonin, dopamine and norepinephrine. Although there are seven distinct classes of antidepressants, selective serotonin reuptake inhibitors (SSRIs) and tetracyclic antidepressants are widely prescribed and generally regarded as the first-line drugs in the treatment of depression. However, many physiological roles of some SSRIs appear to be dissociated with the inhibition of serotonin reuptake. For instance, paroxetine, a member of SSRIs and maprotiline, a member of tetracyclic antidepressant, have been shown to induce apoptosis or to prevent other agents from inducing apoptosis in several cell lines. Thus the effects of these two drugs on the apoptosis are still controversial.
The aim of this study is to investigate the molecular mechanisms of paroxetine and maprotiline in induction of cell death in human osteosarcoma and murine neuroblastoma cells. First, WST-1 reduction assays and propidium iodide-staining assays were used to determine cell viability and apoptosis in the presence of paroxetine and maprotiline. Then immunoblotting was used to measure the activity of apoptotic markers
caspase-
3 and mitogen-activated protein kinases (MAPKs) to survey the apoptotic pathways induced by these two antidepressants. The experimental results may be helpful to understand the pharmacological and toxicological effects of these two antidepressants in cells from important organs.
Results showed that paroxetine caused apoptosis via the activation of
caspase-
3 in cultured human osteosarcoma cells (MG63). Although paroxetine could activate the phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK), only SB203580 (a p38 MAPK inhibitor) partially prevented cells from apoptosis. Paroxetine was also found to induce [Ca2+]i increases but pretreatment with BAPTA/AM, a Ca2+ chelator, prevented paroxetine-induced [Ca2+]i increases, and thus did not protect cells from death. These results suggest that paroxetine caused Ca2+-independent apoptosis via the activation of p38 MAPK-associated
caspase-
3 in MG63 cells.
Maprotiline was also found to induce apoptosis through increased
caspase-
3 activation in murine neuroblastoma Neuro-2a cells. Induction of JNK phosphorylation contributed to the activation of
caspase-
3 resulting in maprotiline-induced Neuro-2a cell apoptosis. Thus, it appears that maprotiline induced apoptosis via JNK/
caspase-
3-dependent signaling pathways. Blockage of activation of ERK was found to increase the activation of
caspase-
3 leading to an enhancement of maprotiline-induced apoptosis. These data suggest that ERK was a survival signal to oppose maprotiline-caused apoptotic effect in Neuro-2a cells. Thus the activation of
caspase-
3 by maprotiline appears to depend on the activation of JNK and the inactivation of ERK. [Ca2+]i measurement in the presence of maprotiline showed that the…
Advisors/Committee Members: Shiping He (committee member), Chen-Chih Kao (chair), Chung-Ren Jan (committee member), Jing-Gung Chung (chair), Chen-Fu Shaw (chair).
Subjects/Keywords: human osteosarcoma cells; paroxetine; maprotiline; Ca2+; MAPKs; caspase-3; apoptosis; murine neuroblastoma cells
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chou, C. (2008). Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559
Chicago Manual of Style (16th Edition):
Chou, Chiang-Ting. “Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline.” 2008. Doctoral Dissertation, NSYSU. Accessed April 22, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559.
MLA Handbook (7th Edition):
Chou, Chiang-Ting. “Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline.” 2008. Web. 22 Apr 2021.
Vancouver:
Chou C. Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline. [Internet] [Doctoral dissertation]. NSYSU; 2008. [cited 2021 Apr 22].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559.
Council of Science Editors:
Chou C. Mechanisms of caspase-3 activation in the apoptosis of human osteosarcoma and murine neuroblastoma cells induced by paroxetine and maprotiline. [Doctoral Dissertation]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0627108-233559

NSYSU
26.
Zeng, Jun-Yan.
New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line.
Degree: Master, Biological Sciences, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758
► Antimitotic drug, combretastatin A-4 (CA-4) is a natural plant extract exhibits powerful anticancer and anti-angiogenesis activity by targeting microtubule formation. CA-4 enediyne derivatives, such as…
(more)
▼ Antimitotic drug, combretastatin A-4 (CA-4) is a natural plant extract exhibits powerful anticancer and anti-angiogenesis activity by targeting microtubule formation. CA-4 enediyne derivatives, such as LO-OMe and LO-NH2 were shown to possess potent cytotoxicity in various cancer cell lines except human neuroblastoma cell. Here, we further investigate the cytotoxic effects of LO-OMe and LO-NH2 on neuroblastoma SH-SY5Y cells. Our viability assay demonstrated that LO-OMe and LO-NH2 mediated 50 percent killing of SH-SY5Y cells at a concentration of
3.2 and 2.1 µM, respectively. Cell lysates of drug treated cells were collected for analyzing markers of cellular autophagy and apoptosis using antibodies against LC3, p62,
caspase-
3, cleaved PARP. The results indicated a dose-dependent increase of LC3-II/LC3-I ratio and p62 protein upon drug treatments. In addition, consistent effects were observed in GFP-LC3 reporter H4 cell line. Both compounds elicited the increase in the number and the size of GFP-LC3 puncta, indicating an accumulation of autophagosomes in reporter cells. Our previous data showed LO-OMe and LO-NH2 treatment led to cell cycle arrest at G2/M. In addition, LO-OMe and LO-NH2 also significantly unregulated the level of active
caspase-
3, which resolved in the increase of cleaved-PARP. In summary, our results showed that LO-OMe and LO-NH2 induced the increase of autophagosomes. The accumulation of p62 indicated an inhibition in the fusion between autophagosome and lysosome, which might be due to the interference of microtubule polymerization exerted by these compounds. Unresolved cellular stress then resulted in programmed cell death of SH-SY5Y neuroblastoma cells. However, the molecular details awaits further investigation.
Advisors/Committee Members: Ming-Jung Wu (chair), Jiin-Tsuey Cheng (committee member), Hong Yi-Ren (chair).
Subjects/Keywords: Combretastatin A-4; LO-OMe; LO-NH2; PARP; Caspase-3; Apoptosis; Autophagy; p62; LC3
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zeng, J. (2015). New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zeng, Jun-Yan. “New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line.” 2015. Thesis, NSYSU. Accessed April 22, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zeng, Jun-Yan. “New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line.” 2015. Web. 22 Apr 2021.
Vancouver:
Zeng J. New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Apr 22].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zeng J. New Combretastatin A-4 enediyne derivatives induce Program Cell Death in Neuroblastoma SH-SY5Y cell line. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019115-165758
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria
27.
[No author].
Testicular apoptotic activity in two bio-sentinel fish
species inhabiting an aquatic ecosystem in an area where continual
DDT spraying occurs : utility of immunohistochemical
assays
.
Degree: 2009, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-07082009-171902/
► Endocrine disrupting chemicals (EDCs) such as DDT have the ability to disrupt hormonally controlled processes, such as spermatogenesis, which is the maturation of germ cells…
(more)
▼ Endocrine disrupting chemicals (EDCs) such as DDT
have the ability to disrupt hormonally controlled processes, such
as spermatogenesis, which is the maturation of germ cells into
spermatozoa. During normal spermatogenesis, germ cell apoptosis can
occur, but the degree of apoptosis within the testis could possibly
be affected by exposure to EDCs. In 2004, a pilot study on the
reproductive health of two freshwater fish species, Oreochromis
mossambicus and Clarias gariepinus, from three impoundments in the
Luvuvhu River, found concerning levels of DDT and its metabolites
in both species from the Nandoni Dam, and in O. mossambicus from
the Xikundu Weir. This was not surprising as a large part of the
Luvuvhu River catchment is located within an area where ongoing
DDT-spraying occurs for vector control purposes. Hence, in 2006, a
larger WRC-funded project began to further investigate the findings
from the pilot study. A subsidiary study, spanning two seasons, was
initiated to investigate testicular apoptosis in fish from the
polluted systems, the Nandoni Dam (ND) and the Xikundu Weir (XW),
as well as a reference site, the Albasini Dam (AD), utilizing
caspase-
3 and TUNEL immunoexpression as apoptotic markers. In
addition, three fixatives, Bouin’s Fluid (BF), Neutrally Buffered
Formalin (NBF) and Paraformaldehyde (PFA), were used to determine
which would be the optimal fixative for both histological and
immunohistochemical assessments. Sampling occurred during season 1,
the low-flow season (October 2007), during DDT spraying of the
surrounding area, and season 2, the high–flow season (February
2008), two months after the DDT-spraying was completed. The testes
of O. mossambicus (n = 19 season 1, n = 25 season 2) and C.
gariepinus (n = 19 season 1, n = 20 season 2) were fixed in the
above-mentioned fixatives, embedded in paraffin wax, prepared for
immunohistochemistry, and exposed to
caspase-
3 antibodies and TUNEL
antibodies individually. The results indicated that the residues of
p,p´-DDT - DDD and - DDE were found in the fat samples of both O.
mossambicus and C. gariepinus, in AD, ND and XW. Testicular
apoptotic assessment using the
caspase-
3 assay clearly labeled
spermatocytes in the process of cellular death in both seasons, in
all three fixatives. When comparing the two assays, a significant
difference is found between the
caspase-
3 and TUNEL positive cells.
The results further show that, when comparing the three sampling
sites, the highest amount of positive cells are found at the XW.
The decrease observed in season two, in both the
caspase-
3 and
TUNEL assay may possibly be linked to the stage of spermatogenesis,
coinciding with hormonal changes associated with the different
sampling seasons (i.e. breeding and non-breeding seasons). The
levels of DDT found in the fat tissue, could not be correlated to
an up-regulation in apoptotic cells. The results The results
indicated that the choice of fixative, could affect the
identification of the amount of positive cells. The utility of the
caspase-
3 and TUNEL assays, in…
Advisors/Committee Members: Dr J C Van Dyk (advisor), Prof M S Bornman (advisor).
Subjects/Keywords: Apoptosis;
Caspase-3 immunohistochemistry;
Tunel;
Oreochromis mossambicus;
Clarias gariepinus;
Spermatogenesis;
Ddt;
UCTD
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2009). Testicular apoptotic activity in two bio-sentinel fish
species inhabiting an aquatic ecosystem in an area where continual
DDT spraying occurs : utility of immunohistochemical
assays
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07082009-171902/
Chicago Manual of Style (16th Edition):
author], [No. “Testicular apoptotic activity in two bio-sentinel fish
species inhabiting an aquatic ecosystem in an area where continual
DDT spraying occurs : utility of immunohistochemical
assays
.” 2009. Masters Thesis, University of Pretoria. Accessed April 22, 2021.
http://upetd.up.ac.za/thesis/available/etd-07082009-171902/.
MLA Handbook (7th Edition):
author], [No. “Testicular apoptotic activity in two bio-sentinel fish
species inhabiting an aquatic ecosystem in an area where continual
DDT spraying occurs : utility of immunohistochemical
assays
.” 2009. Web. 22 Apr 2021.
Vancouver:
author] [. Testicular apoptotic activity in two bio-sentinel fish
species inhabiting an aquatic ecosystem in an area where continual
DDT spraying occurs : utility of immunohistochemical
assays
. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Apr 22].
Available from: http://upetd.up.ac.za/thesis/available/etd-07082009-171902/.
Council of Science Editors:
author] [. Testicular apoptotic activity in two bio-sentinel fish
species inhabiting an aquatic ecosystem in an area where continual
DDT spraying occurs : utility of immunohistochemical
assays
. [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-07082009-171902/

Universidade de Brasília
28.
Sílvia Taveira Elias.
Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.
Degree: 2009, Universidade de Brasília
URL: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575
► O câncer é um problema mundial de saúde pública. Nas últimas décadas a incidência de câncer de cabeça e pescoço vem aumentando, sendo os países…
(more)
▼ O câncer é um problema mundial de saúde pública. Nas últimas décadas a incidência de câncer de cabeça e pescoço vem aumentando, sendo os países desenvolvidos os mais afetados. As terapias convencionais para este tipo de câncer, cirurgia e radioterapia, são agressivas e não aumentam de maneira considerável a qualidade e expectativa de vida dos pacientes. Nesse cenário, a descoberta de novas terapias é necessária. Esse trabalho tem como objetivo investigar o efeito citotóxico do extrato bruto de tabaco (EBT) e cinco frações provenientes dele (metanólica, neutra, carbônica, ácida e básica) em células de carcinoma oral humano. A exposição das células de câncer oral ao EBT induz a morte celular e diminui a viabilidade celular de maneira dose-dependente. Das frações testadas, apenas a neutra foi capaz de induzir uma significante morte celular (acima de 50%) após 48 horas de tratamento. Ademais, fragmentação do DNA e ativação da caspase-3 indicam que a morte celular induzida por EBT e pela fração neutra tem natureza apoptótica. Os resultados mostram que apesar do tabaco ser um carcinógeno conhecido, possuir inúmeros componentes indutores de tumor, ele também pode conter componentes capazes de induzir morte em células de câncer. Pelo fato da fração neutra ser capaz de também induzir morte celular, pode-se inferir que o componente apoptótico é apolar. Novos estudos são necessários para avaliar a estrutura química envolvida neste processo.
Cancer is a public health problem worldwide. Incidences of head and neck cancer are increasing in the last decades, and the developed countries are the most affected. Current therapeutic options for this type of cancer are aggressive, including surgery and radiotherapy. In addition, they have not yet translated into an improvement of life quality or expectancy to patients. In this scenario, new therapeuticals are urgently needed and actively sought after. The goal of this study was to investigate the cytotoxic effects of tobacco crude extract (TCE) and 5 fractions thereof (methanolic, neutral, carbonic, acid and basic) on a human oral squamous cell carcinoma. Exposure of oral cancer cells to TCE induced cell death and decreased cell viability in a dose-dependent manner. Out of the tested fractions, only the neutral one was able to induce significant cell death (over 50%) over the period of 48h. In addition, DNA laddering and caspase-3 activation indicate that the cell death processes induced by TCE and neutral fraction were apoptotic in nature. Our results indicate that although tobacco is a known carcinogen, possessing many tumorinitiating compounds, it could also contain compounds that are useful to induce apoptosis in cancer cells. Because the neutral fraction was also able to induce apoptosis, it is postulated that this putative compound is non-polar, although further investigation is needed to uncover its true nature and chemical structure.
Advisors/Committee Members: Eliete Neves da Silva Guerra, Marie Togashi, Andrea Barreto Motoyama, Fabio Pittella Silva.
Subjects/Keywords: apoptose; extrato bruto de tabaco; caspase-3; CIENCIAS DA SAUDE; câncer de boca
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Elias, S. T. (2009). Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. (Thesis). Universidade de Brasília. Retrieved from http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Elias, Sílvia Taveira. “Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.” 2009. Thesis, Universidade de Brasília. Accessed April 22, 2021.
http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Elias, Sílvia Taveira. “Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano.” 2009. Web. 22 Apr 2021.
Vancouver:
Elias ST. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. [Internet] [Thesis]. Universidade de Brasília; 2009. [cited 2021 Apr 22].
Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Elias ST. Efeito apoptótico do extrato bruto de tabaco de carcinoma oral humano. [Thesis]. Universidade de Brasília; 2009. Available from: http://bdtd.bce.unb.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5575
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
29.
M.V. Traversa.
NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB.
Degree: 2011, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/151771
► Hair exerts a range of functions including thermoregulation, physical protection, sensory activity. Mature and actively growing hair follicles become anchored in the subcutis, and regenerate…
(more)
▼ Hair exerts a range of functions including thermoregulation, physical protection,
sensory activity. Mature and actively growing hair follicles become anchored in
the subcutis, and regenerate by spontaneous repetitive cycles of growth
(anagen), apoptosis-driven regression (catagen), and quiescence (telogen). We
focused our study on investigating the effect of new therapeutic molecules
capable of counteracting the regression phase. We have tested our working
hypothesis on HFDPC cells, grown in culture in Follicle Dermal Papilla Growth
Medium, and apoptosis was induced by 24 hours incubation with 1 μM
staurosporin. This treatment resulted in a marked activation of
Caspase-
3
accompanied by cytoskeletal degradation, nuclear blebbing, and cellular
fragmentation. The addition of spermidin or rutine in the micromolar range
concentration reduced staurosporin-induced
caspase activity by over 50%,
when the two agents were added simultaneously equal levels of
caspase-
3
inhibition was achieved with concentration 10 fold lower. Zeaxantine alone was
ineffective, however when added to the combined spermidin and rutine
treatment, the staurosporin-induced
caspase activity was almost totally
counteracted and the enzymatic activity was significantly reduced. These
combined treatment was also effective in preventing staurosporin-mediated
cellular damage. The extent of cell loss was greatly reduced and there was a
total preservation and normal distribution of actin-tubulin cytoskeleton with
normal cellular shape. Interestingly, our combined treatment also counteracted
caspase-
3 over-expression induced by staurosporin. In conclusion, this
sperimental molecules can counteract HFDPC cells apoptosis and may
represent an effective preventing treatment for the catagen phase of the hair
bulb life cycle.
Advisors/Committee Members: docente guida: A. Gorio, coordinatore: A. M. Di Giulio, GORIO, ALFREDO, DI GIULIO, ANNA MARIA.
Subjects/Keywords: HFDP cells; androgenetic alopecia; apoptosis; caspase-3; non synthetic molecules; Settore BIO/14 - Farmacologia
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Traversa, M. (2011). NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/151771
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Traversa, M.V.. “NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB.” 2011. Thesis, Università degli Studi di Milano. Accessed April 22, 2021.
http://hdl.handle.net/2434/151771.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Traversa, M.V.. “NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB.” 2011. Web. 22 Apr 2021.
Vancouver:
Traversa M. NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB. [Internet] [Thesis]. Università degli Studi di Milano; 2011. [cited 2021 Apr 22].
Available from: http://hdl.handle.net/2434/151771.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Traversa M. NEW NON SYNTHETIC MOLECULES ARE ABLE TO INHIBIT APOPTOSIS IN DERMAL PAPILLAR CELLS OF HAIR BULB. [Thesis]. Università degli Studi di Milano; 2011. Available from: http://hdl.handle.net/2434/151771
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Wayne State University
30.
Feng, Ke.
An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field.
Degree: PhD, Biomedical Engineering, 2017, Wayne State University
URL: https://digitalcommons.wayne.edu/oa_dissertations/1700
► Blast-induced traumatic brain injury (bTBI) is a signature wound of modern warfare. The current incomplete understanding of its injury mechanism impedes the development of…
(more)
▼ Blast-induced traumatic brain injury (bTBI) is a signature wound of modern warfare. The current incomplete understanding of its injury mechanism impedes the development of strategies for effective protection of bTBI. Despite a considerable amount of experimental animal studies focused on the evaluation of brain neurotrauma caused by blast exposure, there is very limited knowledge on the biomechanical responses of the gyrenecephalic brain subjected to primary free-field blast waves imposed in vivo, and the correlation analysis between the biomechanical responses and its injury outcomes. Such information is crucial to the development of injury criteria of bTBI.
This study aims to evaluate the external and internal mechanical responses of the brain against different levels of blast loading with Yucatan swine in free field, and to conduct correlational studies with brain tissue damage. To better understand primary bTBI, we have implemented an open field experimental model to apply controlled shock waves on swine head. The applied pressure levels of shock waves were predicted by finite element modeling and verified with calibrated testing. Biomechanical responses of primary blasts such as intracranial pressure (ICP), head kinetics, strain rate of skull, were measured in vivo during the blasts. A positive correlation between incident overpressure (IOP) and its corresponding biomechanical responses of the brain was observed. A parallel group of non-instrumented animals were used to collect injury data 72 hours post experiment. Cellular responses governed by primary blasts, such as neuronal degeneration and apoptosis were studied via immunohistochemistry. Representative fluorescent-stained images were examined under microscope. A positive correlation was found between the amount of degenerative neurons and the blast level. Significant elevation of apoptosis was found in the high-level blast. Comparisons between brains with varies ICP readings demonstrate differences of the numbers of neuronal degeneration and apoptosis within the imaged volume. Additionally, comparisons between sections at different locations of the head did not show spatial changes for cellular responses. These metrics provide a pathway for direct connection between the cellular damage and the measured biomechanical responses of the brain within the same experimental model, and could be critical in understanding the mechanisms of bTBI. This experimental data can be used to validate computer models of bTBI.
Advisors/Committee Members: John Cavanaugh.
Subjects/Keywords: Blast; Caspase-3; FJ-C; ICP; swine; TBI; Biomechanics; Nanoscience and Nanotechnology
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Feng, K. (2017). An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/1700
Chicago Manual of Style (16th Edition):
Feng, Ke. “An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field.” 2017. Doctoral Dissertation, Wayne State University. Accessed April 22, 2021.
https://digitalcommons.wayne.edu/oa_dissertations/1700.
MLA Handbook (7th Edition):
Feng, Ke. “An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field.” 2017. Web. 22 Apr 2021.
Vancouver:
Feng K. An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field. [Internet] [Doctoral dissertation]. Wayne State University; 2017. [cited 2021 Apr 22].
Available from: https://digitalcommons.wayne.edu/oa_dissertations/1700.
Council of Science Editors:
Feng K. An Investigation Of The Mechanism Of Traumatic Brain Injury Caused By Blast In The Open Field. [Doctoral Dissertation]. Wayne State University; 2017. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1700
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