subject:(Cartilage)

University of Western Australia

1. Ebert, Jay Robert. Post-operative load bearing rehabilitation following autologous chondrocyte implantation.

Degree: PhD, 2008, University of Western Australia

URL: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10386&local_base=GEN01-INS01

► [Truncated abstract] Autologous Chondrocyte Implantation (ACI) has shown early clinical success as a repair procedure to address focal articular cartilage defects in the knee, and… (more)

▼ [Truncated abstract] Autologous Chondrocyte Implantation (ACI) has shown early clinical success as a repair procedure to address focal articular cartilage defects in the knee, and involves isolating and culturing a patient's own chondrocytes in vitro and re-implantation of those cells into the cartilage defect. Over time, repair tissue can develop and remodel into hyaline-like cartilage. A progressive partial weight bearing (PWB) program becomes the critical factor in applying protection and progressive stimulation of the implanted cells, to promote best chondrocyte differentiation and development, without overloading the graft. The aim of this thesis was to investigate whether patients could replicate this theoretical load bearing model to possibly render the best quality tissue development. In addition, this proposed external load progression is only a means to loading the articular surface. Several factors, including those that may result from pathology, have the potential to influence gait patterns, and therefore, articular loading. The association between increasing external loads (ground reaction forces - GRF) and knee joint kinetics during partial and full weight bearing gait was, therefore, investigated in the ACI patient group, as was the contribution of other gait variables to these knee joint kinetics which may be modified by the clinician. Finally, current weight bearing (WB) protocols have been based on early ACI surgical techniques. With advancement in the surgical procedure and ongoing clinical experience, we employed a randomised controlled clinical trial to assess the effectiveness of an 'accelerated' load bearing program, compared with the traditionally 'conservative' post-operative protocol. ... Although similar spatio-temporal, knee kinematic and external loading parameters were observed between the traditional and accelerated rehabilitation groups, the accelerated group was 'more comparable' to the controls in their external knee adduction and flexion moments, where the traditional group had lower knee moments. Knee moments greatly affect knee articular loading, and large adduction moments have been related to poor clinical outcomes after surgery. Therefore, the return of normal levels may be ideal for graft stimulation, however, may overload the immature chondrocytes. Acceleration of the intensive rehabilitation program will enable the patient to return to normal activities earlier, whilst reducing time and expenses associated with the rehabilitative process, and may enhance long-term tissue development. However, continued follow-up is required to determine if there are any detrimental effects that may emerge as a result of the accelerated load bearing program, and assess the recovery of normal gait patterns and whether longer term graft outcomes are affected by the recovery time course of normal gait function, and/or abnormal loading mechanics in gait. Furthermore, analysis at all levels of PWB is needed to identify a more complete set of variables attributing to the magnitude of external knee…

Subjects/Keywords: Articular cartilage; Cartilage; Knee; Knee

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APA (6^th Edition):

Ebert, J. R. (2008). Post-operative load bearing rehabilitation following autologous chondrocyte implantation. (Doctoral Dissertation). University of Western Australia. Retrieved from http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10386&local_base=GEN01-INS01

Chicago Manual of Style (16^th Edition):

Ebert, Jay Robert. “Post-operative load bearing rehabilitation following autologous chondrocyte implantation.” 2008. Doctoral Dissertation, University of Western Australia. Accessed January 23, 2020. http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10386&local_base=GEN01-INS01.

MLA Handbook (7^th Edition):

Ebert, Jay Robert. “Post-operative load bearing rehabilitation following autologous chondrocyte implantation.” 2008. Web. 23 Jan 2020.

Vancouver:

Ebert JR. Post-operative load bearing rehabilitation following autologous chondrocyte implantation. [Internet] [Doctoral dissertation]. University of Western Australia; 2008. [cited 2020 Jan 23]. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10386&local_base=GEN01-INS01.

Council of Science Editors:

Ebert JR. Post-operative load bearing rehabilitation following autologous chondrocyte implantation. [Doctoral Dissertation]. University of Western Australia; 2008. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10386&local_base=GEN01-INS01

Mississippi State University

2. Gilbert, Eric Andrew. Efficacy of a novel through-thickness perfusion bioreactor to create scaffold-free tissue engineered cartilage.

Degree: MS, Agricultural and Biological Engineering, 2013, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10262013-234246/ ;

► Articular cartilage is an avascular, aneural tissue that covers the ends of diarthroidal joints. Once damaged by disease or injury, cartilage lacks the ability… (more)

Subjects/Keywords: neonatal porcine cartilage; cartilage bioreactor

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APA (6^th Edition):

Gilbert, E. A. (2013). Efficacy of a novel through-thickness perfusion bioreactor to create scaffold-free tissue engineered cartilage. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-10262013-234246/ ;

Chicago Manual of Style (16^th Edition):

Gilbert, Eric Andrew. “Efficacy of a novel through-thickness perfusion bioreactor to create scaffold-free tissue engineered cartilage.” 2013. Masters Thesis, Mississippi State University. Accessed January 23, 2020. http://sun.library.msstate.edu/ETD-db/theses/available/etd-10262013-234246/ ;.

MLA Handbook (7^th Edition):

Gilbert, Eric Andrew. “Efficacy of a novel through-thickness perfusion bioreactor to create scaffold-free tissue engineered cartilage.” 2013. Web. 23 Jan 2020.

Vancouver:

Gilbert EA. Efficacy of a novel through-thickness perfusion bioreactor to create scaffold-free tissue engineered cartilage. [Internet] [Masters thesis]. Mississippi State University; 2013. [cited 2020 Jan 23]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10262013-234246/ ;.

Council of Science Editors:

Gilbert EA. Efficacy of a novel through-thickness perfusion bioreactor to create scaffold-free tissue engineered cartilage. [Masters Thesis]. Mississippi State University; 2013. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10262013-234246/ ;

University of Western Australia

3. Lin, Zhen. Chondrocyte : a target for the treatment of osteoarthritis.

Degree: PhD, 2007, University of Western Australia

URL: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=9342&local_base=GEN01-INS01

►

[Truncated abstract] Osteoarthritis (OA) is the most common form of arthritis, characterized by progressively degeneration of articular cartilage. Chondrocyte is the only cell type in… (more)

▼

[Truncated abstract] Osteoarthritis (OA) is the most common form of arthritis, characterized by progressively degeneration of articular cartilage. Chondrocyte is the only cell type in articular cartilage tissue and responsible for cartilage matrix turnover. This thesis focuses on the biological and genetic behaviors of human chondrocyte and potential therapeutic strategies that target on chondrocyte. Chondrocytes have been used for the tissue-engineered cartilage construction, especially in articular cartilage repair. The technique of chondrocyte-base tissue engineering utilizes in vitro propagated chondrocytes combined with several manufactured biomaterials to regenerate cartilage tissue. Although these technologies have been successfully applied in clinic, the biological characteristics of chondrocyte during in vitro propagation and after implantation remain unclear. This thesis reviewed the present studies of chondrocyte biology and its potential uses in tissue engineering. Particularly, chondrocytes have been shown to de-differentiate into fibroblastic-cells when they are exposed to inflammatory conditions or cultured on monolayer in vitro. This thesis investigated the gene expression profile of chondrocytes when they are cultured and serially passaged on monolayer in vitro. Human chondrocytes obtained from OA patients were cultured up to passage 6. Twenty-eight chondrocyte associated genes were measured by Real-time PCR. The results showed that a number of genes were changed in expression levels at various stages of passage as indications of chondrocyte de-differentiation. Chondrocytes derived from OA patients or normal donors exhibited a very similar gene expression pattern. Interestingly, transcription factor Sox-9, which plays a key role in chondrogenesis remained unchanged with increasing passage number, indicating that the de-differentiation process of chondrocyte is reversible. This thesis also focused on the development of novel pharmacological approaches for OA that target on articular chondrocyte. The clinical feature, etiology, pathogenesis, diagnostic approaches, conventional and potential future treatments for OA were briefly reviewed in this thesis. ... The effects of natural compounds on chondrocyte gene expression, proteoglycan degradation and nitric oxide production were measured. The results showed that parthenolide, a NF-kB inhibitor, regulated chondrocyte function by suppressing the up-regulation of gene expression of inflammatory factors and matrix proteinases induced by lipopolysaccharide, and down-regulating COX-2 expression. Parthenolide was able to reduce proteoglycan degradation in human chondrocytes, but had no effect on nitric oxide production. These results suggest that parthenolide mediates inflammatory-activated NF-kB pathway, and subsequently reduces inflammatory response, prevents cartilage destruction and relieves pain, and hence may be useful for OA treatment.

[Truncated abstract] Osteoarthritis (OA) is the most common form of arthritis, characterized by progressively…

Subjects/Keywords: Cartilage; Cartilage; Cartilage cells; Osteoarthritis; Tissue engineering; Chondrocyte; Cartilage; Osteoarthritis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, Z. (2007). Chondrocyte : a target for the treatment of osteoarthritis. (Doctoral Dissertation). University of Western Australia. Retrieved from http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=9342&local_base=GEN01-INS01

Chicago Manual of Style (16^th Edition):

Lin, Zhen. “Chondrocyte : a target for the treatment of osteoarthritis.” 2007. Doctoral Dissertation, University of Western Australia. Accessed January 23, 2020. http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=9342&local_base=GEN01-INS01.

MLA Handbook (7^th Edition):

Lin, Zhen. “Chondrocyte : a target for the treatment of osteoarthritis.” 2007. Web. 23 Jan 2020.

Vancouver:

Lin Z. Chondrocyte : a target for the treatment of osteoarthritis. [Internet] [Doctoral dissertation]. University of Western Australia; 2007. [cited 2020 Jan 23]. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=9342&local_base=GEN01-INS01.

Council of Science Editors:

Lin Z. Chondrocyte : a target for the treatment of osteoarthritis. [Doctoral Dissertation]. University of Western Australia; 2007. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=9342&local_base=GEN01-INS01

4. Mandl, Erik. Advances in cartilage tissue engineering : in vitro.

Degree: 2004, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/39804

► textabstractWithin the body three subtypes of cartilage can be distinguished: hyaline cartilage, elastic cartilage and fibrocartilage. Hyaline cartilage is the predominant subtype and is mainly… (more)

▼ textabstractWithin the body three subtypes of cartilage can be distinguished: hyaline cartilage, elastic cartilage and fibrocartilage. Hyaline cartilage is the predominant subtype and is mainly located in articular joints and in less extent in the nasal septum and cricoid. Elastic cartilage can be found in the outer ear and in parts of the respiratory tract. The menisci and intervertebral discs are made from the third subtype: fibrocartilage. Hyaline cartilage in joints, further refered to as articular cartilage, is a highly specialized tissue that ensures low-friction movement of articulating bones, whilst transmitting load. The only cell-type present in articular cartilage is the chondrocyte, which only composes less than 5% of the total tissue volume. Water (up to 80% wet weight) is the predominant molecule in cartilage, followed by different collagens (10-20% wet weight) and the group of GAG (GlycosAminoGlycan, 4-7% wet weight) 181. From the latter two groups, collagen type II and aggrecan are the most prominent respectively. A simplified reflection of the cartilage structure and function would be as follows. The collagen molecules form a tightly woven network in which the GAG are contained (Figure I-1 next page). In solution, the GAG is negatively charged and creates an osmotic pressure via attraction of positive counter-ions. This osmotic pressure, together with repulsive forces of the negatively charged GAG, result in a swelling pressure within the matrix that accounts for the excellent capabilities of cartilage to absorb and transmit forces during joint movement and loading. Several smaller molecules also play a crucial role in the extracellular matrix of articular cartilage: decorin, anchorin, collagen type VI, IX and XI. Articular cartilage lacks blood vessels; chondrocytes receive oxygen and nutrients through diffusion from the synovium and the underlying bone. Articular cartilage can be divided in four zones with respect to the architecture 68, 181, 213 (Figure I-2, next page). The superficial zone comprises approximately 10% of the cartilage and is characterized by a high cell density, flattened chondrocytes and thin collagen fibers that are oriented tangential to the articular surface. The middle zone or intermediate zone has randomly orientated collagen fibers and chondrocytes with a more spherical configuration.

Subjects/Keywords: cartilage

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APA (6^th Edition):

Mandl, E. (2004). Advances in cartilage tissue engineering : in vitro. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/39804

Chicago Manual of Style (16^th Edition):

Mandl, Erik. “Advances in cartilage tissue engineering : in vitro.” 2004. Doctoral Dissertation, Erasmus University Medical Center. Accessed January 23, 2020. http://hdl.handle.net/1765/39804.

MLA Handbook (7^th Edition):

Mandl, Erik. “Advances in cartilage tissue engineering : in vitro.” 2004. Web. 23 Jan 2020.

Vancouver:

Mandl E. Advances in cartilage tissue engineering : in vitro. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 2004. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1765/39804.

Council of Science Editors:

Mandl E. Advances in cartilage tissue engineering : in vitro. [Doctoral Dissertation]. Erasmus University Medical Center; 2004. Available from: http://hdl.handle.net/1765/39804

5. Stone, Austin V. A potential biologic role for the meniscus in the development of osteoarthritis.

Degree: 2013, Wake Forest University

URL: http://hdl.handle.net/10339/39030

► The overarching goal of this research is to identify the biologic role of the meniscus in the development of osteoarthritis. We hypothesized that inflammatory factors… (more)

Subjects/Keywords: cartilage

…monkey menisci. ....................................... 35 Table S1: Vervet knee cartilage and… …72 Figure 3: Comparison of osteoarthritic meniscus and cartilage cells in response to… …78 Table S1: Grading system used for cartilage and meniscus specimens… …120 Table S1: Grading system for cartilage and meniscus specimens… …leads to a compromise of the structural integrity of the cartilage, subchondral bone, and the…

11 more images…

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APA (6^th Edition):

Stone, A. V. (2013). A potential biologic role for the meniscus in the development of osteoarthritis. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/39030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stone, Austin V. “A potential biologic role for the meniscus in the development of osteoarthritis.” 2013. Thesis, Wake Forest University. Accessed January 23, 2020. http://hdl.handle.net/10339/39030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stone, Austin V. “A potential biologic role for the meniscus in the development of osteoarthritis.” 2013. Web. 23 Jan 2020.

Vancouver:

Stone AV. A potential biologic role for the meniscus in the development of osteoarthritis. [Internet] [Thesis]. Wake Forest University; 2013. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/10339/39030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stone AV. A potential biologic role for the meniscus in the development of osteoarthritis. [Thesis]. Wake Forest University; 2013. Available from: http://hdl.handle.net/10339/39030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

6. Biasutti, Sara. Experimental Studies in Tendinopathy and Use of a Novel Tendon Autograft for Cartilage Resurfacing: An Ovine Model .

Degree: 2018, University of Sydney

URL: http://hdl.handle.net/2123/18621

► Cartilage damage and osteoarthritis are enormous medical problems in humans and animals. The inherent poor regenerative capacity of articular cartilage after injury results in loss… (more)

Subjects/Keywords: Cartilage; tendon; tendinopathy; osteoarthritis; cartilage resurfacing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Biasutti, S. (2018). Experimental Studies in Tendinopathy and Use of a Novel Tendon Autograft for Cartilage Resurfacing: An Ovine Model . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/18621

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Biasutti, Sara. “Experimental Studies in Tendinopathy and Use of a Novel Tendon Autograft for Cartilage Resurfacing: An Ovine Model .” 2018. Thesis, University of Sydney. Accessed January 23, 2020. http://hdl.handle.net/2123/18621.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Biasutti, Sara. “Experimental Studies in Tendinopathy and Use of a Novel Tendon Autograft for Cartilage Resurfacing: An Ovine Model .” 2018. Web. 23 Jan 2020.

Vancouver:

Biasutti S. Experimental Studies in Tendinopathy and Use of a Novel Tendon Autograft for Cartilage Resurfacing: An Ovine Model . [Internet] [Thesis]. University of Sydney; 2018. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/2123/18621.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Biasutti S. Experimental Studies in Tendinopathy and Use of a Novel Tendon Autograft for Cartilage Resurfacing: An Ovine Model . [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/18621

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

7. Griffin, Darvin. Assessing The Mechanical Behavior Of Treatments For Focal Articular Cartilage Lesions .

Degree: 2016, Cornell University

URL: http://hdl.handle.net/1813/43588

► In a healthy knee joint, articular cartilage (AC) supports loads, dissipates energy, and lubricates with little to no signs of wear or damage. With injury… (more)

▼ In a healthy knee joint, articular cartilage (AC) supports loads, dissipates energy, and lubricates with little to no signs of wear or damage. With injury or degeneration related to osteoarthritis (OA), cartilage changes occur leading to significant loss of mechanical function, with the potential to cause further progressive degeneration of cartilage. While the connection between mechanical changes and arthritis progression is well known, previous work has focused primarily on bulk, tissue-scale ([ALMOST EQUAL TO]1mm) behavior. What is less clear is how mechanical behavior changes on the microscale (e.g. [ALMOST EQUAL TO]10-20[MICRO SIGN]m) during cartilage degradation. Previous work in our lab has developed techniques for measuring local strains in healthy cartilage via confocal elastography. This work focuses on applying traditional and novel techniques to understand the mechanical behavior at degraded and repaired articular cartilage. The first aim elucidates the fundamental relationships between the composition and structure of degraded cartilage and its local mechanical behavior, specifically its viscoelastic response with degeneration. This study combined state of the art techniques for analyzing cartilage structure, and high resolution mapping of mechanical properties on the microscale([ALMOST EQUAL TO]20[MICRO SIGN]m). This work provided new insight into structural and local mechanical changes that occur in cartilage during the early stages of OA. Due to its avascular nature, articular cartilage exhibits an extremely limited capacity to heal when damaged. Consequently, research dealing with cartilage repair strategies is of elevated importance. Restoring the mechanical properties of tissue at the repair site is a common problem in tissue engineering techniques aimed at repairing cartilage defects. Therefore, the second and third aims investigated the mechanical performance of repaired cartilage treated with either matrix membranes or growth factors to assist in tissue formation within a defect site and surrounding AC. The present work has developed into an innovative mechanical characterization technique. Specifically, combining the confocal elastography technique, with the unique sample populations from second and third aim to tackle a problem that has faced the orthopedic research field for more than two decades: understanding the mechanics of the interface between native and repaired cartilage. I've identified two distinct error modes of failure for this interface - sliding and peeling. As such, understanding the structure function relationship in healthy, damaged, and repaired cartilage, is critical for devising strategies to restore tissue impaired by injury or disease and can provide a template for successful implant design. Advisors/Committee Members: Cohen,Itai (committeeMember), Nixon,Alan J (committeeMember).

Subjects/Keywords: Articular Cartilage; Cartilage Repair; Confocal Elastography

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APA (6^th Edition):

Griffin, D. (2016). Assessing The Mechanical Behavior Of Treatments For Focal Articular Cartilage Lesions . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/43588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Griffin, Darvin. “Assessing The Mechanical Behavior Of Treatments For Focal Articular Cartilage Lesions .” 2016. Thesis, Cornell University. Accessed January 23, 2020. http://hdl.handle.net/1813/43588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Griffin, Darvin. “Assessing The Mechanical Behavior Of Treatments For Focal Articular Cartilage Lesions .” 2016. Web. 23 Jan 2020.

Vancouver:

Griffin D. Assessing The Mechanical Behavior Of Treatments For Focal Articular Cartilage Lesions . [Internet] [Thesis]. Cornell University; 2016. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1813/43588.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Griffin D. Assessing The Mechanical Behavior Of Treatments For Focal Articular Cartilage Lesions . [Thesis]. Cornell University; 2016. Available from: http://hdl.handle.net/1813/43588

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

8. Yao, Qing, Angela. Cellular stress pathways in cartilage biology and disease.

Degree: PhD, 2015, University of Hong Kong

URL: Yao, Q. A. [姚青]. (2015). Cellular stress pathways in cartilage biology and disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435637 ; http://dx.doi.org/10.5353/th_b5435637 ; http://hdl.handle.net/10722/223687

► Cells experience many intrinsic and extrinsic stress factors and respond by activating specific pathways for survival. In many human diseases and normal developmental processes, cross… (more)

▼ Cells experience many intrinsic and extrinsic stress factors and respond by activating specific pathways for survival. In many human diseases and normal developmental processes, cross talks between cellular stress pathways are part of the regulatory system determining cell fate. It is well established that the presence of misfolded proteins in the endoplasmic reticulum (ER) activate the unfolded protein response (UPR) as part of the ER-stress signal for cell survival. Furthermore, other stress pathways in cells utilize the UPR as part of the cascade of events for cell survival. For example, while hypoxia activates HIF proteins, components of UPR are also utilized for cell survival. However, whether UPR mediating from the ER stress has a direct relationship with the hypoxia response or the HIF pathway is not known. Here, using a mouse model for metaphyseal chondrodyspalsia type Schmid (MCDS) with activation of ER-stress in hypertrophic chondrocyte due to the expression of misfolded collagen X in the developing growth plate, we showed that the hypoxia pathway could indeed be utilized by the UPR response, with the PERK sensor and ATF4 in the stabilization of HIF proteins for cell survival. Specifically, we showed that accumulation of mutant collagen type X (13del) proteins in the ER of hypertrophic chondrocytes resulted in a stabilization of HIF proteins and nuclear localization. This event is a cell autonomous confirmed in mouse chimeras containing a mixture of both normal and 13del MCDS hypertrophic chondrocytes. While the mechanism of HIF protein stabilization is still not clear, it is likely to be a stabilization of HIF proteins already present in chondrocytes, as the growth plate cartilage is avascular. Thus, the HIF proteins are captured by hypertrophic chondrocytes under ER stress as part of the survival mechanism. That HIF proteins are needed for survival of MCDS hypertrophic chondrocyte is consistent with our finding that a conditional inactivation of the Hif-1α gene in hypertrophic chondrocytes results in a mild induction of apoptosis. However, the role of Hif2α still needs to be addressed, as it is likely to have a redundant role with Hif1α. A link to ATF4 was provided through the generation of Col10a1-ATF transgenic mice with ectopic expression of ATF4 in hypertrophic chondrocytes under the regulation of the Col10a1 promoter. The result is an expanded zone of hypertrophic chondrocytes similar to 13del-MCDS mice and more interestingly, a stabilization of HIF proteins that are localized in the nucleus. However, the mechanism and precise link in this instance require further study of the molecular links and validation of interplay. Finally, we showed that hypertrophic chondrocytes under ER stress induced a change in the metabolic process that is consistent with a hypoxia condition. It is likely that the metabolic changes require more oxygen than is available in the environment, such that the cellular changes mimic a hypoxia outcome, providing a positive signal using EF5 as an indicator. My findings…

Subjects/Keywords: Stress (Physiology); Cartilage

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APA (6^th Edition):

Yao, Qing, A. (2015). Cellular stress pathways in cartilage biology and disease. (Doctoral Dissertation). University of Hong Kong. Retrieved from Yao, Q. A. [姚青]. (2015). Cellular stress pathways in cartilage biology and disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435637 ; http://dx.doi.org/10.5353/th_b5435637 ; http://hdl.handle.net/10722/223687

Chicago Manual of Style (16^th Edition):

Yao, Qing, Angela. “Cellular stress pathways in cartilage biology and disease.” 2015. Doctoral Dissertation, University of Hong Kong. Accessed January 23, 2020. Yao, Q. A. [姚青]. (2015). Cellular stress pathways in cartilage biology and disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435637 ; http://dx.doi.org/10.5353/th_b5435637 ; http://hdl.handle.net/10722/223687.

MLA Handbook (7^th Edition):

Yao, Qing, Angela. “Cellular stress pathways in cartilage biology and disease.” 2015. Web. 23 Jan 2020.

Vancouver:

Yao, Qing A. Cellular stress pathways in cartilage biology and disease. [Internet] [Doctoral dissertation]. University of Hong Kong; 2015. [cited 2020 Jan 23]. Available from: Yao, Q. A. [姚青]. (2015). Cellular stress pathways in cartilage biology and disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435637 ; http://dx.doi.org/10.5353/th_b5435637 ; http://hdl.handle.net/10722/223687.

Council of Science Editors:

Yao, Qing A. Cellular stress pathways in cartilage biology and disease. [Doctoral Dissertation]. University of Hong Kong; 2015. Available from: Yao, Q. A. [姚青]. (2015). Cellular stress pathways in cartilage biology and disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435637 ; http://dx.doi.org/10.5353/th_b5435637 ; http://hdl.handle.net/10722/223687

Cornell University

9. Galley, Natalie. Alterations In Tribologic Behavior Of Cartilage During Tissue Degeneration And Repair .

Degree: 2012, Cornell University

URL: http://hdl.handle.net/1813/29437

► Osteoarthritis is the leading cause of disability in the United States, associated with joint pain and loss of mobility of an increasing proportion of the… (more)

Subjects/Keywords: cartilage; friction; osteoarthritis

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APA (6^th Edition):

Galley, N. (2012). Alterations In Tribologic Behavior Of Cartilage During Tissue Degeneration And Repair . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/29437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Galley, Natalie. “Alterations In Tribologic Behavior Of Cartilage During Tissue Degeneration And Repair .” 2012. Thesis, Cornell University. Accessed January 23, 2020. http://hdl.handle.net/1813/29437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Galley, Natalie. “Alterations In Tribologic Behavior Of Cartilage During Tissue Degeneration And Repair .” 2012. Web. 23 Jan 2020.

Vancouver:

Galley N. Alterations In Tribologic Behavior Of Cartilage During Tissue Degeneration And Repair . [Internet] [Thesis]. Cornell University; 2012. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1813/29437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Galley N. Alterations In Tribologic Behavior Of Cartilage During Tissue Degeneration And Repair . [Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/29437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Chicago

10. Stoia, Jonathan L. Cartilage-on-Cartilage Articulating System as a Wear Benchmark for Artificial Replacement Materials.

Degree: 2012, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/9057

► A multidisciplinary study – with applications in engineering, tribology, biomechanics, orthopedics, biochemistry and biology – was conducted to establish a wear system that could be… (more)

▼ A multidisciplinary study – with applications in engineering, tribology, biomechanics, orthopedics, biochemistry and biology – was conducted to establish a wear system that could be used to critically evaluate candidate artificial materials for clinical application in diarthrodial joint rehabilitation. More specifically, this study was conducted to examine the effects of articulating artificial materials against live cartilage by direct comparison to a model that articulated cartilage on cartilage. The goal of this study was met by addressing the following specific aims: 1) To modify the current material-on-cartilage setup to accommodate a cartilage-on-cartilage articulating system; 2) To investigate the effects of using synovial fluid as a lubricant in material-on-cartilage wear experiments as opposed to culture media; 3) To establish a cartilage-on-cartilage articulating system as a benchmark for material-on-cartilage wear experiments. Wear testing was performed in which cobalt chromium alloy (CoCrMo) balls or cartilage strips secured to polymer adapters were articulated against flat cartilage explants. All samples were cultured in constant temperature (37 ºC), humidity (95%) and CO2 level (5%) five days prior to wear testing. In order to apply load (40 N) and motion (±30° ball rotation; 5.2 mm migrating contact) a joint-motion simulator was used. A total of 16200 cycles at 0.5 Hz equally spread over three testing days was applied. A current method of articulating material on cartilage was modified to accommodate articulation of cartilage strips against flat cartilage explants. It was observed that synovial fluid performed significantly better than culture media as a lubricant with respect to maintaining the integrity of the cartilage matrix. It was also observed that cell viability of the explants significantly decreased when synovial fluid was used as the lubricant. The cartilage-on-cartilage model performed significantly better than the material-on-cartilage model with respect to maintaining matrix integrity, cell viability and surface topography characteristics during wear testing. Overall, the findings of this study have improved the current tribological methods of evaluating articular cartilage as well as candidate artificial materials for use in clinical joint rehabilitation treatments. Specifically, this study is directly applicable to clinical studies that facilitate hemiarthroplasties. The findings of this study may also lead to further development of critical evaluation methods and treatments involving articular cartilage and artificial materials. Advisors/Committee Members: Wimmer, Markus (advisor).

Subjects/Keywords: Articular cartilage; biotribology

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APA (6^th Edition):

Stoia, J. L. (2012). Cartilage-on-Cartilage Articulating System as a Wear Benchmark for Artificial Replacement Materials. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/9057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stoia, Jonathan L. “Cartilage-on-Cartilage Articulating System as a Wear Benchmark for Artificial Replacement Materials.” 2012. Thesis, University of Illinois – Chicago. Accessed January 23, 2020. http://hdl.handle.net/10027/9057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stoia, Jonathan L. “Cartilage-on-Cartilage Articulating System as a Wear Benchmark for Artificial Replacement Materials.” 2012. Web. 23 Jan 2020.

Vancouver:

Stoia JL. Cartilage-on-Cartilage Articulating System as a Wear Benchmark for Artificial Replacement Materials. [Internet] [Thesis]. University of Illinois – Chicago; 2012. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/10027/9057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stoia JL. Cartilage-on-Cartilage Articulating System as a Wear Benchmark for Artificial Replacement Materials. [Thesis]. University of Illinois – Chicago; 2012. Available from: http://hdl.handle.net/10027/9057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

IUPUI

11. Chandwadkar, Shaunak A. Indentation protocol to determine viscoelastic properties of cartilage before and after crosslinking.

Degree: 2017, IUPUI

URL: http://hdl.handle.net/1805/15092

►

Indiana University-Purdue University Indianapolis (IUPUI)

Osteoarthritis affects millions of people of different age groups around the world. With very few treatment options and the highly… (more)

Subjects/Keywords: CASPc; Cartilage crosslinking

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APA (6^th Edition):

Chandwadkar, S. A. (2017). Indentation protocol to determine viscoelastic properties of cartilage before and after crosslinking. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/15092

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chandwadkar, Shaunak A. “Indentation protocol to determine viscoelastic properties of cartilage before and after crosslinking.” 2017. Thesis, IUPUI. Accessed January 23, 2020. http://hdl.handle.net/1805/15092.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chandwadkar, Shaunak A. “Indentation protocol to determine viscoelastic properties of cartilage before and after crosslinking.” 2017. Web. 23 Jan 2020.

Vancouver:

Chandwadkar SA. Indentation protocol to determine viscoelastic properties of cartilage before and after crosslinking. [Internet] [Thesis]. IUPUI; 2017. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1805/15092.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chandwadkar SA. Indentation protocol to determine viscoelastic properties of cartilage before and after crosslinking. [Thesis]. IUPUI; 2017. Available from: http://hdl.handle.net/1805/15092

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queen Mary, University of London

12. Thomas, Bethan. WNT16 in chondrocyte biology and lineage determination.

Degree: PhD, 2015, Queen Mary, University of London

URL: http://qmro.qmul.ac.uk/xmlui/handle/123456789/9870 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775127

► Background. During development WNT16 is a specific marker of the superficial zone of the developing articular cartilage. In adult joints WNT16 is no longer expressed,… (more)

▼ Background. During development WNT16 is a specific marker of the superficial zone of the developing articular cartilage. In adult joints WNT16 is no longer expressed, but it becomes rapidly upregulated in the articular cartilage following injury and in osteoarthritis. Unpublished data from our laboratory show that WNT16 deficient mice are more susceptible to instability-induced osteoarthritis. The superficial zone of the articular cartilage contains chondrogenic progenitor cells which are essential for the long-term maintenance of cartilage homeostasis. These cells express WNT16 and also specifically express Lubricin. Lubricin, in adulthood, is essential for joint lubrication and in its absence, mice develop spontaneous osteoarthritis. Results. Exogenous WNT16 dose-dependently increased Lubricin expression in wnt16-/- superficial zone cells and primary bovine chondrocytes. WNT16 treatment also dose-dependently modulated Axin2, a transcriptional target of the canonical Wnt pathway. At low concentration Wnt16 downregulated axin2 expression, while higher concentrations caused upregulation compared to control. WNT16 also caused a dose-dependent phosphorylation of c-Jun transcription factor. In keeping with my data in chondrocytes, in xenopus laevis experiments, WNT16 had a limited capacity to induce features of axis duplication, but could efficiently inhibit axis duplication induced by WNT8. Importantly, both DKK1 and TCS (inhibitors of the canonical Wnt pathway and of the JNK pathways, respectively) prevented the WNT16-induced Lubricin upregulation, thereby demonstrating that modulation of the gene by WNT16 requires both the canonical Wnt pathway and the JNK pathways. WNT16 supported the phenotype of superficial zone cells by enhancing Lubricin expression and by preventing their full chondrocytic maturation: extracellular matrix production was increased in wnt16-/- superficial zone cells and chondrocyte specific marker were lost upon WNT16 stimulation in these cells as well as in bovine primary chondrocytes. Conclusion. WNT16 is a weak activator of the canonical Wnt pathway which supports lubricin expression and the phenotype of the cartilage superficial zone cells.

Subjects/Keywords: WNT16; Articular cartilage

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APA (6^th Edition):

Thomas, B. (2015). WNT16 in chondrocyte biology and lineage determination. (Doctoral Dissertation). Queen Mary, University of London. Retrieved from http://qmro.qmul.ac.uk/xmlui/handle/123456789/9870 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775127

Chicago Manual of Style (16^th Edition):

Thomas, Bethan. “WNT16 in chondrocyte biology and lineage determination.” 2015. Doctoral Dissertation, Queen Mary, University of London. Accessed January 23, 2020. http://qmro.qmul.ac.uk/xmlui/handle/123456789/9870 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775127.

MLA Handbook (7^th Edition):

Thomas, Bethan. “WNT16 in chondrocyte biology and lineage determination.” 2015. Web. 23 Jan 2020.

Vancouver:

Thomas B. WNT16 in chondrocyte biology and lineage determination. [Internet] [Doctoral dissertation]. Queen Mary, University of London; 2015. [cited 2020 Jan 23]. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/9870 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775127.

Council of Science Editors:

Thomas B. WNT16 in chondrocyte biology and lineage determination. [Doctoral Dissertation]. Queen Mary, University of London; 2015. Available from: http://qmro.qmul.ac.uk/xmlui/handle/123456789/9870 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.775127

University of Kansas

13. Beck, Emily Claire. Development of Chondroinductive Hydrogel Pastes from Naturally Derived Cartilage Matrix.

Degree: PhD, Bioengineering, 2015, University of Kansas

URL: http://hdl.handle.net/1808/20930

► The main focus of hydrogel technology is on hydrogels in their crosslinked form. Although hydrogels are promising materials for cartilage tissue engineering, the clinical translation… (more)

▼ The main focus of hydrogel technology is on hydrogels in their crosslinked form. Although hydrogels are promising materials for cartilage tissue engineering, the clinical translation of these materials are hindered because they lack the ability to be molded into a defect site by a surgeon due to hydrogel precursors being liquid solutions that are prone to leaking from the implantation site during placement. Therefore, the current thesis work focuses on the hydrogels in their precursor form prior to crosslinking and describes the development of creating hydrogel pastes that have the potential to be clinically translatable. The current thesis first developed a platform hydrogel paste composed of methacrylated hyaluronic acid (MeHA), which is a more traditional hydrogel material, and hyaluronic acid nanoparticles. The hyaluronic acid nanoparticles were shown to impart a yield stress on the hydrogel precursors, allowing the precursors to be molded and shaped prior to crosslinking. Furthermore, the mixtures containing hyaluronic acid nanoparticles were able to be crosslinked and further characterized as solids and they could encapsulate bone marrow-derived stem cells that remained viable. The next major focus of the thesis was tailoring the platform system for cartilage tissue specifically, by gradually replacing each of the two components of the platform system with naturally derived cartilage extracellular matrix, to create a chondroinductive material. Devitalized (DVC) and decellularized cartilage (DCC) particles were found to impart paste-like behavior in MeHA gels, where DVC significantly upregulated chondrogenic gene expression. DCC that was solubilized and methacrylated (MeSDCC) was created and crosslinked, which formed hydrogels with a compressive modulus in the range of native cartilage tissue. Finally, DVC particles mixed in with solubilized and methacrylated DVC created pastes that significantly upregulated chondrogenic gene expression compared to gels without DVC particles. The important next steps will be to further evaluate these MeSDVC and DVC particle pastes in an in vivo model, and further explore whether decellularization of the tissue is necessary. Ultimately, this thesis successfully developed a hydrogel paste that is inherently chondroinductive and promising for future cartilage tissue engineering applications. Advisors/Committee Members: Detamore, Michael S. (advisor), Berkland, Cory J. (cmtemember), Gerke, Stevin H. (cmtemember), Kieweg, Sarah L. (cmtemember), Andrews, Brian T. (cmtemember).

Subjects/Keywords: Biomedical engineering; cartilage matrix; chondroinduction; decelluarized cartilage; devitalized cartilage; hydrogel paste; tissue engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Beck, E. C. (2015). Development of Chondroinductive Hydrogel Pastes from Naturally Derived Cartilage Matrix. (Doctoral Dissertation). University of Kansas. Retrieved from http://hdl.handle.net/1808/20930

Chicago Manual of Style (16^th Edition):

Beck, Emily Claire. “Development of Chondroinductive Hydrogel Pastes from Naturally Derived Cartilage Matrix.” 2015. Doctoral Dissertation, University of Kansas. Accessed January 23, 2020. http://hdl.handle.net/1808/20930.

MLA Handbook (7^th Edition):

Beck, Emily Claire. “Development of Chondroinductive Hydrogel Pastes from Naturally Derived Cartilage Matrix.” 2015. Web. 23 Jan 2020.

Vancouver:

Beck EC. Development of Chondroinductive Hydrogel Pastes from Naturally Derived Cartilage Matrix. [Internet] [Doctoral dissertation]. University of Kansas; 2015. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1808/20930.

Council of Science Editors:

Beck EC. Development of Chondroinductive Hydrogel Pastes from Naturally Derived Cartilage Matrix. [Doctoral Dissertation]. University of Kansas; 2015. Available from: http://hdl.handle.net/1808/20930

Universiteit Utrecht

14. Rijswijk, J.W. van. Soluble Mediators in Cartilage Regeneration.

Degree: 2013, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/278456

► Osteoarthritis (OA) is an increasing problem in orthopedics. Many factors may contribute to its development, including mechanically induced impact trauma, normal wear and tear, joint… (more)

Subjects/Keywords: Articular cartilage; Osteoarthritis; Regeneration; Cartilage repair; Cartilage biology; Soluble mediators; Stem cells

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APA (6^th Edition):

Rijswijk, J. W. v. (2013). Soluble Mediators in Cartilage Regeneration. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/278456

Chicago Manual of Style (16^th Edition):

Rijswijk, J W van. “Soluble Mediators in Cartilage Regeneration.” 2013. Masters Thesis, Universiteit Utrecht. Accessed January 23, 2020. http://dspace.library.uu.nl:8080/handle/1874/278456.

MLA Handbook (7^th Edition):

Rijswijk, J W van. “Soluble Mediators in Cartilage Regeneration.” 2013. Web. 23 Jan 2020.

Vancouver:

Rijswijk JWv. Soluble Mediators in Cartilage Regeneration. [Internet] [Masters thesis]. Universiteit Utrecht; 2013. [cited 2020 Jan 23]. Available from: http://dspace.library.uu.nl:8080/handle/1874/278456.

Council of Science Editors:

Rijswijk JWv. Soluble Mediators in Cartilage Regeneration. [Masters Thesis]. Universiteit Utrecht; 2013. Available from: http://dspace.library.uu.nl:8080/handle/1874/278456

University of Alberta

15. Hahn, Joshua N. Optimization of Vitrification for Human Articular Cartilage.

Degree: MS, Department of Surgery, 2015, University of Alberta

URL: https://era.library.ualberta.ca/files/2b88qg163

► Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no… (more)

▼ Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no cure for osteoarthritis, and the current treatments are all lacking in some form or another. One treatment which attempts to prevent degeneration of cartilage injury into osteoarthritis is osteochondral allografting. This surgery involves the transplantation of healthy bone and cartilage to replace damaged and diseased areas. Osteochondral allografting application is limited primarily by the supply of fresh, healthy tissue and the lack of a long-term storage method that maintains cell viability within cartilage. Vitrification is a method of cryopreservation that preserves cells and tissues at temperatures low enough to halt all biological activity while maintaining cell health when applied properly. Previous work within this lab resulted in successful vitrification of human articular cartilage, but there is room for improvement. The current research was performed to explore the use of additive compounds as well as the use of a vitrification protocol with altered cryoprotectant exposure criteria in an attempt to improve the post-warmed health of the cryopreserved cartilage tissue. The use of chondroitin sulphate, tetramethylpyrazine, a combination of these two, ascorbic acid, and glucosamine was investigated in a set of cryoprotectant toxicity mitigation experiments. We found that when evaluating the effect of exposure to these compounds in a toxic cryoprotectant solution coupled with a two-day incubation, that all but the chondroitin sulphate alone were capable of improving tissue health, while there were no benefits seen when evaluated before the incubation period. The use of additive compounds has been shown to reduce long-term deleterious effects of CPA exposure, indicating that their use may be beneficial to a vitrification application due to the high CPA concentrations involved. This thesis also experimentally explored an altered cryoprotectant protocol proposed by another student, Nadia Shardt, who used Fick’s 1-D law of diffusion to determine the minimum time required for the diffusion of cryoprotectants into articular cartilage in concentrations that were adequate for vitrification. These modifications reduced the protocol length by one and a half hours, but did not result in viability results that were significantly improved over the standard protocol. As the experimental trials in this thesis work all produced a recovery cell viability that is much lower than the previously published results for the standard vitrification protocol, no conclusions can be made regarding which protocol to use based on these data. The experimental groups did not have an obvious deleterious effect on cell viability and, therefore, the reduction in protocol time may be beneficial. The standard vitrification protocol for articular cartilage has shown good results. The experiments performed here demonstrate that there are two potential avenues that may be exploited to enhance cell…

Subjects/Keywords: Articular Cartilage; Vitrification; Cryopreservation

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APA (6^th Edition):

Hahn, J. N. (2015). Optimization of Vitrification for Human Articular Cartilage. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2b88qg163

Chicago Manual of Style (16^th Edition):

Hahn, Joshua N. “Optimization of Vitrification for Human Articular Cartilage.” 2015. Masters Thesis, University of Alberta. Accessed January 23, 2020. https://era.library.ualberta.ca/files/2b88qg163.

MLA Handbook (7^th Edition):

Hahn, Joshua N. “Optimization of Vitrification for Human Articular Cartilage.” 2015. Web. 23 Jan 2020.

Vancouver:

Hahn JN. Optimization of Vitrification for Human Articular Cartilage. [Internet] [Masters thesis]. University of Alberta; 2015. [cited 2020 Jan 23]. Available from: https://era.library.ualberta.ca/files/2b88qg163.

Council of Science Editors:

Hahn JN. Optimization of Vitrification for Human Articular Cartilage. [Masters Thesis]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/2b88qg163

Texas A&M University

16. Lucia, Jessica Lauren. Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the Ability of Oral Glucosamine to Mitigate these Alterations in Young Horses.

Degree: 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/149284

► This project established an in vivo method to identify and manipulate expression of markers of osteoarthritis (OA). Specifically, strategies that predictably induce joint inflammation to… (more)

▼ This project established an in vivo method to identify and manipulate expression of markers of osteoarthritis (OA). Specifically, strategies that predictably induce joint inflammation to evaluate dietary methods of OA prevention in young horses have yet to be accomplished. Therefore, the 3 studies described herein were conducted to determine effectiveness of an intra-articular lipopolysaccharide (LPS) challenge on markers of inflammation and cartilage metabolism in young horses and potential of dietary glucosamine hydrochloride (HCl) to mitigate these alterations. In the first study, horses were challenged with 0.25 ng or 0.50 ng of intra-articular LPS solution or lactated ringer?s solution (control). Injection of LPS increased inflammation based on synovial prostaglandin E2 (PGE2) concentrations. Carboxypeptide of type II collagen (CPII), a maker of type II collagen synthesis, also increased in a dose-dependent manner. However, clinical parameters of health were not influenced and remained within normal ranges. Carpal circumference increased in response to repeated arthrocentesis. Lameness scores increased with LPS injection when compared to controls. This model of joint inflammation (0.5 ng LPS) was used in the second study to evaluate potential chondroprotective effects of oral glucosamine HCl supplementation in yearling horses. Specifically, the oral absorption of glucosamine HCl versus saline was determined by nasogastric dosing and incorporation of dietary glucosamine HCl into plasma and synovial fluid over time. Plasma and synovial fluid concentrations of glucosamine tended to increase over the 98-d period. In the third study, yearlings were challenged with intra-articular LPS to determine the potential of glucosamine HCl to mitigate inflammation when compared to contralateral joints. Injection of LPS increased synovial PGE2 and cartilage biomarkers CPII and collagenase cleavage neopeptide (C2C), a marker of type II collagen degradation. Oral glucosamine HCl decreased PGE2 and C2C concentrations, but increased levels of CPII. Results of these 3 studies provide a clearer understanding of joint inflammation and cartilage turnover in young horses and demonstrated a potential role of oral glucosamine to mitigate these effects and possibly prevent OA in horses. Advisors/Committee Members: Coverdale, Josie A (advisor), Welsh, Thomas H (committee member), Arnold, Carolyn E (committee member), Heird, James C (committee member).

Subjects/Keywords: lipopolysaccharide; cartilage; inflammation; horse

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APA (6^th Edition):

Lucia, J. L. (2013). Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the Ability of Oral Glucosamine to Mitigate these Alterations in Young Horses. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lucia, Jessica Lauren. “Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the Ability of Oral Glucosamine to Mitigate these Alterations in Young Horses.” 2013. Thesis, Texas A&M University. Accessed January 23, 2020. http://hdl.handle.net/1969.1/149284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lucia, Jessica Lauren. “Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the Ability of Oral Glucosamine to Mitigate these Alterations in Young Horses.” 2013. Web. 23 Jan 2020.

Vancouver:

Lucia JL. Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the Ability of Oral Glucosamine to Mitigate these Alterations in Young Horses. [Internet] [Thesis]. Texas A&M University; 2013. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1969.1/149284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lucia JL. Influence of an Intra-articular Lipopolysaccharide Challenge on Markers of Inflammation and Cartilage Metabolism and the Ability of Oral Glucosamine to Mitigate these Alterations in Young Horses. [Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

17. Kahn, Meredith K. Age-Related Effects on Markers of Inflammation and Cartilage Metabolism in Response to an Intra-Articular Lipopolysaccharide Challenge.

Degree: 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/154113

► Eighteen Quarter Horses were utilized in a randomized complete design for a 28 d experiment to evaluate age-related effects on inflammation and cartilage turnover after… (more)

▼ Eighteen Quarter Horses were utilized in a randomized complete design for a 28 d experiment to evaluate age-related effects on inflammation and cartilage turnover after induction of a single inflammatory insult using lipopolysaccharide (LPS). Horses were grouped by age, with yearlings (yearling; n = 3 males, n = 3 females), 2 to 3 yr olds (2/3; n = 2 males, n = 4 females), and mature 5 to 8 yr olds (mature; n = 2 males, n = 4 females). On d 0, all horses were housed individually and fed diets that met or exceeded NRC (2007) requirements. On d 14, horses were challenged with an intra-articular injection of LPS. Carpal joints were randomly assigned to receive 0.5 ng LPS solution obtained from E. coli O55:B5, or 0.8mL sterile lactated Ringer?s solution as a contralateral control. Synovial fluid was collected prior to LPS injection at pre-injection h 0 (PIH 0) and 6, 12, 24, 168, and 336 h post-injection. Samples were later analyzed using commercial ELISA kits for prostaglandin E2 (PGE2), collagenase cleavage neoepitope (C2C), and carboxypropeptide of type II collagen (CPII). Heart rate (HR), respiratory rate (RR), and rectal temperature (RT) were monitored over the first 24 h and carpal circumference and surface temperature were recorded with additional measurements at 168 and 336 h. Data were analyzed using PROC MIXED procedure of SAS. Values for RT, HR, and RR were within normal range. HR and RT were influenced by age (P < 0.01), while RR was unaffected by age (P ? 0.21). Joint circumference was not influenced by age (P = 0.84), but circumference and surface temperature increased (P < 0.01) over time across all age groups. Synovial PGE2 concentrations tended (P = 0.09) to be influenced by age with yearlings having lower (P = 0.03) concentrations than mature horses. Synovial C2C concentrations were affected by age with yearlings and 2/3 yr olds having lower (P < 0.01) concentrations than mature horses. Concentrations of synovial CPII were influenced by age with yearlings and 2/3 yr old having lower (P ? 0.02) concentrations than mature horses. Ratios of CPII:C2C were influenced by age with mature and 2/3 yr old horses having increased (P < 0.01) values compared to yearlings. These results indicate that inflammation and corresponding cartilage turnover in response to LPS administration vary with age. Advisors/Committee Members: Coverdale, Josie A (advisor), Lucia, Jessica L. (committee member), Welsh, Thomas H. (committee member), Wickersham, Tryon A. (committee member).

Subjects/Keywords: Age; Horse; Lipopolysaccharide; Inflammation; Cartilage

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APA (6^th Edition):

Kahn, M. K. (2014). Age-Related Effects on Markers of Inflammation and Cartilage Metabolism in Response to an Intra-Articular Lipopolysaccharide Challenge. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/154113

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kahn, Meredith K. “Age-Related Effects on Markers of Inflammation and Cartilage Metabolism in Response to an Intra-Articular Lipopolysaccharide Challenge.” 2014. Thesis, Texas A&M University. Accessed January 23, 2020. http://hdl.handle.net/1969.1/154113.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kahn, Meredith K. “Age-Related Effects on Markers of Inflammation and Cartilage Metabolism in Response to an Intra-Articular Lipopolysaccharide Challenge.” 2014. Web. 23 Jan 2020.

Vancouver:

Kahn MK. Age-Related Effects on Markers of Inflammation and Cartilage Metabolism in Response to an Intra-Articular Lipopolysaccharide Challenge. [Internet] [Thesis]. Texas A&M University; 2014. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1969.1/154113.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kahn MK. Age-Related Effects on Markers of Inflammation and Cartilage Metabolism in Response to an Intra-Articular Lipopolysaccharide Challenge. [Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/154113

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

18. 李鍵成; Lee, Kin-shing. In vivo study of asporin function in cartilage tissues.

Degree: PhD, 2014, University of Hong Kong

URL: Lee, K. [李鍵成]. (2014). In vivo study of asporin function in cartilage tissues. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5185915 ; http://dx.doi.org/10.5353/th_b5185915 ; http://hdl.handle.net/10722/207898

► Asporin (ASPN) is a risk factor for osteoarthritis and intervertebral disc degeneration. Its expression increases with aging and degeneration. D14 (14 aspartate-repeat polymorphism) is the… (more)

▼ Asporin (ASPN) is a risk factor for osteoarthritis and intervertebral disc degeneration. Its expression increases with aging and degeneration. D14 (14 aspartate-repeat polymorphism) is the risk allele and D13 is the most common allele. In vitro studies suggest that Asporin functions as a negative regulator of Tgf-β signaling, an important stimulator of matrix formation in bone and cartilage. However, the in vivo role of Asporin in development or its involvement in the pathogenesis of degenerative cartilage diseases is unclear. Here, we use mouse as a model to study the impact of Asporin in the intervertebral discs of the spine. In wide type mice, we showed that Asporin is expressed and localized in the nucleus pulposus and annulus fibrosis of intervertebral discs, and the articular cartilage in knee joints. Furthermore, Asporin expressing cells in these tissues are active in Tgf-β signaling, suggesting a relationship between Asporin and Tgf-β signaling and a role in disc and articular joint maintenance. Using natural degeneration with aging, and models for induced degeneration in the mouse-tail discs, Asporin expression was shown to be up-regulated in nucleus pulposus and annulus fibrosis cells of degenerating intervertebral discs. These cells are also active in Tgf-β signaling supporting a potential relationship with the pathogenesis of disc degeneration. Transgenic mice overexpressing Asporin in cartilage tissues were generated to study this relationship and the impact on the differentiation and function of disc cells. Interestingly, overexpression of Asporin in the nucleus pulposus leads to enhanced production and deposition of extracellular matrix such as glycosaminoglycans, with concomitant changes in cell morphology, suggesting Asporin altered the extracellular matrix niche of resident nucleus pulposus cells. However, such changes are only observed in discs in the tail region but not in lumbar discs. We propose a relationship to mechanical loading as an environmental factor. Molecular analysis of transgene expressing cells showed Tgf-β signaling is active and its downstream target genes up-regulated. Furthermore, overexpression of Asporin enhances differentiation of notochordal-like cells (NCCs) in mouse nucleus pulposus toward the more mature nucleus pulposus cells (NPCs) and chondrocyte-like cells (CLCs) that are more abundant in the human nucleus pulposus and other larger animals that prompt to intervertebral disc degeneration. This study provided new insights into the function of Asporin in the pathogenesis of intervertebral disc degeneration. We proposed a model whereby Asporin, as a genetic risk factor, alters the extracellular environment of the nucleus pulposus, that in conjunction with environmental factors such as mechanical loading, enhances Tgf-β signaling, and consequentially, promotes the maturation of NCCs towards NPCs and CLCs, a hallmark of degenerative process proposed in human and other larger animal models. These transgenic mice provide the opportunity to better understand the…

Subjects/Keywords: Cartilage - Diseases - Genetic aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

李鍵成; Lee, K. (2014). In vivo study of asporin function in cartilage tissues. (Doctoral Dissertation). University of Hong Kong. Retrieved from Lee, K. [李鍵成]. (2014). In vivo study of asporin function in cartilage tissues. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5185915 ; http://dx.doi.org/10.5353/th_b5185915 ; http://hdl.handle.net/10722/207898

Chicago Manual of Style (16^th Edition):

李鍵成; Lee, Kin-shing. “In vivo study of asporin function in cartilage tissues.” 2014. Doctoral Dissertation, University of Hong Kong. Accessed January 23, 2020. Lee, K. [李鍵成]. (2014). In vivo study of asporin function in cartilage tissues. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5185915 ; http://dx.doi.org/10.5353/th_b5185915 ; http://hdl.handle.net/10722/207898.

MLA Handbook (7^th Edition):

李鍵成; Lee, Kin-shing. “In vivo study of asporin function in cartilage tissues.” 2014. Web. 23 Jan 2020.

Vancouver:

李鍵成; Lee K. In vivo study of asporin function in cartilage tissues. [Internet] [Doctoral dissertation]. University of Hong Kong; 2014. [cited 2020 Jan 23]. Available from: Lee, K. [李鍵成]. (2014). In vivo study of asporin function in cartilage tissues. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5185915 ; http://dx.doi.org/10.5353/th_b5185915 ; http://hdl.handle.net/10722/207898.

Council of Science Editors:

李鍵成; Lee K. In vivo study of asporin function in cartilage tissues. [Doctoral Dissertation]. University of Hong Kong; 2014. Available from: Lee, K. [李鍵成]. (2014). In vivo study of asporin function in cartilage tissues. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5185915 ; http://dx.doi.org/10.5353/th_b5185915 ; http://hdl.handle.net/10722/207898

University of Hong Kong

19. Wu, Cheuk-bun, Benny. Micro/nano-mechanics of cartilage with osteoarthritis.

Degree: M. Phil., 2011, University of Hong Kong

URL: Wu, C. B. [胡卓斌]. (2011). Micro/nano-mechanics of cartilage with osteoarthritis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4732210 ; http://dx.doi.org/10.5353/th_b4732210 ; http://hdl.handle.net/10722/174379

►

This study aimed to characterize the in-situ mechanical property and morphology of individual collagen fibril in osteoarthritic (OA) cartilage using indentation-type atomic force microscopy (IT-AFM).… (more)

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This study aimed to characterize the in-situ mechanical property and morphology of individual collagen fibril in osteoarthritic (OA) cartilage using indentation-type atomic force microscopy (IT-AFM). The specimens with intact articular cartilage (AC), mild to severe degenerated OA cartilage were collected with informed consent from the postmenopausal women who underwent hip or knee arthroplasty. The fresh specimens were cryo-sectioned by layers with 50m thick for each from the articular surface to calcified cartilage, and then processed for AFM imaging and nanoindentation test. For each layer, a total of twenty collagen fibrils were randomly selected for testing. AFM tips with the nominal radius less than 10 nm were employed for probing the individual collagen fibril, and the obtained cantilever deflection signal and displacement were recorded for calculating its elastic modulus. Besides AFM nanoindentation, AFM and scanning electron microscopy (SEM) images, haematoxylin & eosin (H&E) staining and micro-indentation were performed on AC to study the changes of ultrastructure and composition between intact AC and OA cartilage. Results showed that an intact AC exhibited a gradation in elastic modulus of collagen fibrils from surface region (2.65±0.31GPa) to bottom region (3.70±0.44GPa). It was noted in the initial stage of OA cartilage that the coefficient of variation for mechanical properties of collagen fibers, ranging from 25~48%, significantly increased as compared with intact one (12%). The thickened and stiffened collagen fibrils initially occurred at either surface region (3.11±0.91GPa) or bottom region (5.64±1.10GPa) with OA progression. Besides thickens, alteration of D-periodic banding patterns of collagen fibrils was observed. It was echoed by fibrotic changes of surface region and tidemark irregularities. On the contrast, the micromechanical properties of cartilage decreased while AC suffered from OA. This result revealed the different approachs of nano and micro-mechanical properties changes in AC. In summary, the alteration of mechanical properties of collagen fibrils started from calcified cartilage as well as articular surface during OA onset, and the low compliance of thickened collagen fibrils deteriorated along disease progression. This study also reveals that the outstanding ability by AFM, in investigating the structure and mechanical properties of collagen fibrils and AC in nanometer scale, is impressive and this nanotechnological instrument is worth to be expected in further development for clinical use.

published_or_final_version

Mechanical Engineering

Master

Master of Philosophy

Advisors/Committee Members: Tang, B, Lu, WW.

Subjects/Keywords: Articular cartilage.; Osteoarthritis - Treatment.; Collagen.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, Cheuk-bun, B. (2011). Micro/nano-mechanics of cartilage with osteoarthritis. (Masters Thesis). University of Hong Kong. Retrieved from Wu, C. B. [胡卓斌]. (2011). Micro/nano-mechanics of cartilage with osteoarthritis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4732210 ; http://dx.doi.org/10.5353/th_b4732210 ; http://hdl.handle.net/10722/174379

Chicago Manual of Style (16^th Edition):

Wu, Cheuk-bun, Benny. “Micro/nano-mechanics of cartilage with osteoarthritis.” 2011. Masters Thesis, University of Hong Kong. Accessed January 23, 2020. Wu, C. B. [胡卓斌]. (2011). Micro/nano-mechanics of cartilage with osteoarthritis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4732210 ; http://dx.doi.org/10.5353/th_b4732210 ; http://hdl.handle.net/10722/174379.

MLA Handbook (7^th Edition):

Wu, Cheuk-bun, Benny. “Micro/nano-mechanics of cartilage with osteoarthritis.” 2011. Web. 23 Jan 2020.

Vancouver:

Wu, Cheuk-bun B. Micro/nano-mechanics of cartilage with osteoarthritis. [Internet] [Masters thesis]. University of Hong Kong; 2011. [cited 2020 Jan 23]. Available from: Wu, C. B. [胡卓斌]. (2011). Micro/nano-mechanics of cartilage with osteoarthritis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4732210 ; http://dx.doi.org/10.5353/th_b4732210 ; http://hdl.handle.net/10722/174379.

Council of Science Editors:

Wu, Cheuk-bun B. Micro/nano-mechanics of cartilage with osteoarthritis. [Masters Thesis]. University of Hong Kong; 2011. Available from: Wu, C. B. [胡卓斌]. (2011). Micro/nano-mechanics of cartilage with osteoarthritis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4732210 ; http://dx.doi.org/10.5353/th_b4732210 ; http://hdl.handle.net/10722/174379

University of Hong Kong

20. Fong, Man-kit. An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages.

Degree: M. Phil., 2012, University of Hong Kong

URL: Fong, M. [方文傑]. (2012). An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775314 ; http://dx.doi.org/10.5353/th_b4775314 ; http://hdl.handle.net/10722/174494

► Osteoarthritis (OA) is one of the most concerned diseases in the field of orthopaedics. During the process of this disease, articular cartilages are degenerated and… (more)

▼ Osteoarthritis (OA) is one of the most concerned diseases in the field of orthopaedics. During the process of this disease, articular cartilages are degenerated and worn out at the end stage, which create pain and disabilities to the patients. Although multiple mechanical and biochemical factors may initiate and/or enhance the progression of OA, alternation of biomechanical properties of articular cartilage is one of the products. There are several major components in articular cartilage; it is believed that each of their contribution cannot be entirely neglected. Superficial zone is mainly consisted with collagen and it was found that the biomechanical properties of this part of cartilage are also alternated significantly as a result of OA. Hence, degeneration of collagen network also occurs. Alternatively, osteoporosis (OP) is another common disease, which is associated to the decrease of bone mineral density; the effect of OP on articular cartilage is limited. In reverse, increasing bone mineral density in subchondral plate alters the loads distribution on articular cartilage and possibly leads to OA. This current study investigated the morphological and biomechanical properties of individual collagen fibrils extracted from OA, OP and healthy cartilages. A total of ten joint specimens were recruited, 3 OA joints were from 3 OA patients, 3 OP joints were from 3 OP patients, and 4 joints were from 2 healthy individuals. All cartilages were harvested from non weight-bearing zone, and average diameters were calculated from 350 fibrils’ measurements. In addition, 50 fibrils were randomly selected for nano-indentation under ambient condition. However, the representation of biomechanical properties tested at low humidity may be questionable. This current study also investigated the stiffness of hydrated fibrils. The results showed that the collagen fibrils extracted from OA cartilages were thinner than the ones extracted from OP and healthy cartilages. It was believe that the fibrillation and derangement of collagen network spread from superficial zone towards deeper zones. However, the number of thinner collagen fibrils increased in OA specimens could be the reason of the loss of larger fibrils and/or fragmentation occurred in the superficial zone, where contains thinner fibrils. The biomechanical tests showed that fibrils extracted from OA cartilages owned the highest elastic modulus, while the ones from OP had the lowest; significant differences were found between all groups when tested under ambient condition. Alternatively, the same pattern of results could also be found when hydrated fibrils were tested; however, due to the limited amount of samples, only the difference between OA and OP were considered significant. In addition, no individual difference was found; no significant difference between samples within each group could be observed. Since nano-indentations were performed at the center of each fibril, the elastic moduli measured represented the stiffness of the crosslinks and molecules within fibrils.… Advisors/Committee Members: Lu, WW, Ngan, AHW, Tang, B, Chan, D.

Subjects/Keywords: Articular cartilage - Physiology.; Collagen.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fong, M. (2012). An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. (Masters Thesis). University of Hong Kong. Retrieved from Fong, M. [方文傑]. (2012). An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775314 ; http://dx.doi.org/10.5353/th_b4775314 ; http://hdl.handle.net/10722/174494

Chicago Manual of Style (16^th Edition):

Fong, Man-kit. “An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages.” 2012. Masters Thesis, University of Hong Kong. Accessed January 23, 2020. Fong, M. [方文傑]. (2012). An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775314 ; http://dx.doi.org/10.5353/th_b4775314 ; http://hdl.handle.net/10722/174494.

MLA Handbook (7^th Edition):

Fong, Man-kit. “An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages.” 2012. Web. 23 Jan 2020.

Vancouver:

Fong M. An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. [Internet] [Masters thesis]. University of Hong Kong; 2012. [cited 2020 Jan 23]. Available from: Fong, M. [方文傑]. (2012). An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775314 ; http://dx.doi.org/10.5353/th_b4775314 ; http://hdl.handle.net/10722/174494.

Council of Science Editors:

Fong M. An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. [Masters Thesis]. University of Hong Kong; 2012. Available from: Fong, M. [方文傑]. (2012). An investigation of biomechanical properties of collagen fibrils extracted from osteoarthritic and osteoporotic cartilages. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775314 ; http://dx.doi.org/10.5353/th_b4775314 ; http://hdl.handle.net/10722/174494

University of Hong Kong

21. 赵伟玮; Zhao, Weiwei. Role of endothelin-1 in chondrocyte differentiation and cartilage homeostasis.

Degree: PhD, 2017, University of Hong Kong

URL: http://hdl.handle.net/10722/244289

►

Currently, osteoarthritis (OA) is recognized as a heterogeneous disease with a variety of causes. The initiation and progression of OA cannot be accounted for by… (more)

▼

Currently, osteoarthritis (OA) is recognized as a heterogeneous disease with a variety of causes. The initiation and progression of OA cannot be accounted for by mechanical stress alone. There is an increasing body of evidence showing that systemic conditions, such as chronic inflammation and metabolic disorders, contribute to the development of OA. Given the high frequency of OA’s co-occurrence with cardiovascular diseases (CVDs) and metabolic syndrome (MetS), special attention has been paid to studying the common mechanisms underlying those systemic diseases. Unraveling the common biological pathways would benefit our understanding of the pathogenesis of metabolic OA as well as the development of novel drugs for OA treatment. The view that endothelin-1 (ET-1) plays a critical role in the cardiovascular system arose from a number of observations that levels of plasma ET-1 were found to be significantly upregulated in patients with CVDs and MetS. More recently, it was suggested that ET-1 promoted cartilage degradation via collagenase upregulation. Thereby, we hypothesized that the alteration of systemic ET-1 would affect the vascular functions as well as the cartilage homeostasis. In this study, we aimed to investigate the role of ET-1 in chondrocyte differentiation and cartilage homeostasis. The predominant expression of ET-1 in hypertrophic chondrocytes was detected, suggesting the association of ET-1 signaling with chondrocyte hypertrophy. The overexpression of endothelial ET-1 resulted in slight dwarfism in neonatal mice, which was attributed to the activity of ET-1 in endochondral ossification initiated by the hypertrophic differentiation of chondrocytes. Age-related spontaneous cartilage degeneration was found in transgenic mice overexpressing endothelial ET-1 (TET-1 mice), suggesting that the elevation of systemic ET-1 affected the homeostasis of articular cartilage. An increased number of type Ⅹ collagen positive chondrocytes in un-calcified cartilage was identified. Furthermore, accelerated progression of traumatic OA was observed in TET-1 mice. In vitro study showed that ET-1 facilitated chondrocyte hypertrophy by increasing the expression of hypertrophic markers. These results suggested increased ET-1 promoted cartilage degeneration probably via the regulation of chondrocyte hypertrophy. Taken together, we have demonstrated the role of systemic ET-1 in chondrocyte hypertrophy, endochondral ossification, cartilage maintenance, and OA. This is the first in vivo study to investigate the effect of elevated systemic ET-1 on chondrocytes and cartilage, contributing to the knowledge about ET-1’s role in the skeletal system.

published_or_final_version

Orthopaedics and Traumatology

Doctoral

Doctor of Philosophy

Subjects/Keywords: Cartilage cells; Cell differentiation; Endothelins

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APA (6^th Edition):

赵伟玮; Zhao, W. (2017). Role of endothelin-1 in chondrocyte differentiation and cartilage homeostasis. (Doctoral Dissertation). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/244289

Chicago Manual of Style (16^th Edition):

赵伟玮; Zhao, Weiwei. “Role of endothelin-1 in chondrocyte differentiation and cartilage homeostasis.” 2017. Doctoral Dissertation, University of Hong Kong. Accessed January 23, 2020. http://hdl.handle.net/10722/244289.

MLA Handbook (7^th Edition):

赵伟玮; Zhao, Weiwei. “Role of endothelin-1 in chondrocyte differentiation and cartilage homeostasis.” 2017. Web. 23 Jan 2020.

Vancouver:

赵伟玮; Zhao W. Role of endothelin-1 in chondrocyte differentiation and cartilage homeostasis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2017. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/10722/244289.

Council of Science Editors:

赵伟玮; Zhao W. Role of endothelin-1 in chondrocyte differentiation and cartilage homeostasis. [Doctoral Dissertation]. University of Hong Kong; 2017. Available from: http://hdl.handle.net/10722/244289

University of Aberdeen

22. Jeffrey, Janet Elizabeth. The response of articular cartilage to impact loading.

Degree: 2009, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25206 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499679

► In this study an <i>in vitro</i> model was used to simulate joint trauma by subjecting explants of articular cartilage to a single impact load using… (more)

Subjects/Keywords: 612; Articular cartilage : Osteoarthritis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jeffrey, J. E. (2009). The response of articular cartilage to impact loading. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25206 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499679

Chicago Manual of Style (16^th Edition):

Jeffrey, Janet Elizabeth. “The response of articular cartilage to impact loading.” 2009. Doctoral Dissertation, University of Aberdeen. Accessed January 23, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25206 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499679.

MLA Handbook (7^th Edition):

Jeffrey, Janet Elizabeth. “The response of articular cartilage to impact loading.” 2009. Web. 23 Jan 2020.

Vancouver:

Jeffrey JE. The response of articular cartilage to impact loading. [Internet] [Doctoral dissertation]. University of Aberdeen; 2009. [cited 2020 Jan 23]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25206 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499679.

Council of Science Editors:

Jeffrey JE. The response of articular cartilage to impact loading. [Doctoral Dissertation]. University of Aberdeen; 2009. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=25206 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499679

University of Rochester

23. Shen, Run; Chen, Di (1955 - ). Regulation of Runx2 protein stability in chondrocyte development.

Degree: PhD, 2009, University of Rochester

URL: http://hdl.handle.net/1802/6536

► During endochondral bone formation, cartilages provide the template for vascular invasion, which brings in osteoblasts to deposit bone matrix on the debris of apoptotic chondrocytes.… (more)

▼ During endochondral bone formation, cartilages provide the template for vascular invasion, which brings in osteoblasts to deposit bone matrix on the debris of apoptotic chondrocytes. Chondrocytes are basic building units of cartilage. Their proliferation and differentiation direct the development of cartilage, by first generating a sufficient number of chondrocytes and then switching to many mature protein mills. PTHrP is one of the essential growth factors which coordinate the whole cartilage developmental program, so that cartilage achieves proper size and maturation state at different stages. PTHrP has been reported to promote chondrocyte proliferation by upregulating cyclin D1 gene expression and preventing chondrocyte differentiation, while the molecular mechanism of these regulations is largely unknown. To understand this, we conducted both in vitro biochemical and in vivo genetic studies. Runx2 is a critical transcriptional factor which promotes chondrocyte maturation. By protein sequence analysis and biochemical mapping for critical domains, we identified serine 472 at the C-terminal of Runx2 is a potential phosphorylation site by cyclin D1/CDK4 complex, and its phosphorylation is essential for inducing Runx2 protein turnover. Mutation of this serine to alanine abolished the phosphorylation of Runx2 and made it resistant to cyclin D1-induced protein turnover, and correlated with higher protein stability of Runx2. In vivo, we demonstrated that PTHrP treatment of chondrocytes correlates with a decrease of Runx2 protein level. The growth plate of the cyclin D1 knockout mouse showed a much smaller proliferative zone and advanced maturation at hypertrophic zone. Chondrocytes isolated from cyclin D1 null mice showed higher protein level of Runx2 and lost response to PTHrP stimulation. In summary, PTHrP prevents chondrocyte maturation by inducing Runx2 protein turnover through upregulation of cyclin D1/CDK4 activity. The proliferation and differentiation of chondrocytes are inversely correlated in cartilage development, and this study first provides a molecular mechanism of how proliferation and differentiation are coordinated through protein phosphorylation, ubiquitin-dependent degradation.

Subjects/Keywords: Ubiquitin; Runx2; Chondrocyte; PTHrP; Cartilage

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shen, Run; Chen, D. (. -. ). (2009). Regulation of Runx2 protein stability in chondrocyte development. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/6536

Chicago Manual of Style (16^th Edition):

Shen, Run; Chen, Di (1955 - ). “Regulation of Runx2 protein stability in chondrocyte development.” 2009. Doctoral Dissertation, University of Rochester. Accessed January 23, 2020. http://hdl.handle.net/1802/6536.

MLA Handbook (7^th Edition):

Shen, Run; Chen, Di (1955 - ). “Regulation of Runx2 protein stability in chondrocyte development.” 2009. Web. 23 Jan 2020.

Vancouver:

Shen, Run; Chen D(-). Regulation of Runx2 protein stability in chondrocyte development. [Internet] [Doctoral dissertation]. University of Rochester; 2009. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1802/6536.

Council of Science Editors:

Shen, Run; Chen D(-). Regulation of Runx2 protein stability in chondrocyte development. [Doctoral Dissertation]. University of Rochester; 2009. Available from: http://hdl.handle.net/1802/6536

University of Rochester

24. Gao, Lin (1976 - ). The Role of TAK1 Mediated Signaling in Cartilage Development and Disease.

Degree: PhD, 2012, University of Rochester

URL: http://hdl.handle.net/1802/25536

► Cartilage is a connective tissue required for the growth of a developing animal and serves as the cushion for joints. In longitudinal bone development, endochondral… (more)

▼ Cartilage is a connective tissue required for the growth of a developing animal and serves as the cushion for joints. In longitudinal bone development, endochondral ossification converts cartilage anlagen into bone through sequential stages from mesenchymal cell condensation to chondrocyte proliferation, hypertrophy and terminal maturation, followed by osteoblasts invasion and bone matrix deposition. Disruption of any of these developmental stages leads to cartilage and bone defects. Better understanding of the mechanisms underlying cartilage development and maintenance will make it possible for better medical care for cartilage and skeletal disease. Tgfβ and BMP are secreted ligands belonging to the Tgfβ superfamily growth factors, and have various roles in cartilage physiological and pathological processes. Tgfβ superfamily members bind to serine/threonine kinase type I and type II receptors and signal through canonical and noncanonical signaling pathways. This dissertation aims to define the roles of noncanonical Tgfβ and BMP signal transduction in postnatal cartilage development and homeostasis. To achieve this goal, BMP type I receptors were assessed, in which Alk3 shows the potential to mediate BMP and Tgfβ induced TAK1-P38-ATF2 signaling pathway and has an effect on canonical BMP and Tgfβ signaling, which argues that in addition to being a BMP type I receptor, Alk3 bridges both Tgfβ and BMP to cytoplasmic signals. In a second set of experiments, cartilage specific, inducible TAK1 loss of function mice were generated. Loss of TAK1 at one week shows severe growth retardation at one month. Analysis of the knee joint cartilage shows loss of proteoglycans, decreased Type II collagen and Aggrecan I expression and reduced chondrocyte proliferation. Additionally, expression of Sox9, Sox5 and Sox6, master regulators of chondrogenesis and chondrocyte proliferation was suppressed upon deletion of TAK1. The signaling mechanism by which TAK1 regulates Sox9 expression was also assessed. The TAK1-P38-ATF2 signaling pathway works synergistically with the canonical Smad-dependent BMP signaling pathway on the regulation of Sox9 gene expression. Overall, this dissertation sheds lights on the mechanism of TAK1 mediated signaling in postnatal cartilage development and homeostasis and may contribute to the development of novel therapeutics for cartilage diseases such as chondrodysplasisa and osteoarthritis (OA).

Subjects/Keywords: TAK1; Cartilage; Development; Disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, L. (. -. ). (2012). The Role of TAK1 Mediated Signaling in Cartilage Development and Disease. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/25536

Chicago Manual of Style (16^th Edition):

Gao, Lin (1976 - ). “The Role of TAK1 Mediated Signaling in Cartilage Development and Disease.” 2012. Doctoral Dissertation, University of Rochester. Accessed January 23, 2020. http://hdl.handle.net/1802/25536.

MLA Handbook (7^th Edition):

Gao, Lin (1976 - ). “The Role of TAK1 Mediated Signaling in Cartilage Development and Disease.” 2012. Web. 23 Jan 2020.

Vancouver:

Gao L(-). The Role of TAK1 Mediated Signaling in Cartilage Development and Disease. [Internet] [Doctoral dissertation]. University of Rochester; 2012. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1802/25536.

Council of Science Editors:

Gao L(-). The Role of TAK1 Mediated Signaling in Cartilage Development and Disease. [Doctoral Dissertation]. University of Rochester; 2012. Available from: http://hdl.handle.net/1802/25536

University of Rochester

25. Kohn, Anat. The Role of RBPjκ-Dependent Notch Signaling During Cartilage Development of the Limb Skeleton.

Degree: PhD, 2013, University of Rochester

URL: http://hdl.handle.net/1802/27308

► Cartilage development within the limb skeleton is initiated by the condensation of mesenchymal progenitor cells within the developing limb bud. These progenitors undergo chondrogenesis to… (more)

▼ Cartilage development within the limb skeleton is initiated by the condensation of mesenchymal progenitor cells within the developing limb bud. These progenitors undergo chondrogenesis to generate immature chondrocytes and the cartilage template from which individual skeletal elements form. Chondrocytes must undergo a highly organized process of proliferation, differentiation, and cell death in order to achieve normal skeletal growth and function. These processes of chondrocyte proliferation, hypertrophic and terminal hypertrophic differentiation, and cartilage matrix turnover are intricately regulated by developmental signaling pathways that include: Ihh/PTHrP, Wnt/β-catenin, BMPs and FGFs. Importantly, many of these genetic pathways are defective in congenital and age related cartilage diseases (ie. chondrodysplasias and osteoarthritis), and therefore elucidating the molecular mechanisms by which cartilage develops is crucial to our understanding of cartilage disorders, the repair response to cartilage injury, and age associated changes observed in growth plate and joint cartilages. Recently, our group and others identified the Notch signaling pathway as yet another important regulator of cartilage development. The work presented here set out to determine the molecular mechanisms by which Notch signaling regulates chondrocyte proliferation, hypertrophic differentiation, and terminal chondrocyte maturation using both in vivo and in vitro approaches. Specifically, we relied heavily upon mouse genetic approaches using the Cre/loxP system to generate various Notch-related gain- and loss-of-function mouse models for our analyses. Our studies established, for the first time, that RBPjκ-dependent Notch signaling regulates both the onset of chondrocyte hypertrophy and terminal chondrocyte maturation. Surprisingly, the RBPjκ-independent pathway was identified as a regulator of chondrocyte proliferation, and a long-range, cell non-autonomous regulator of perichondral bone formation. Further studies established Sox9, the master transcriptional regulator of chondrogeneis, as a target of RBPjκ-dependent Notch signaling important for Notch-mediated regulation of chondrocyte hypertrophy. Finally, HES1 was identified as at least one of the mediators of RBPjκ-dependent Notch signaling in the regulation of Sox9 and the initiation of hypertrophy. Taken together, this work provides a strong foundation for our molecular understanding of how Notch signaling regulates cartilage development, allowing future studies to bring even further clarification to the role of Notch signaling in both normal cartilage development and disease.

Subjects/Keywords: NOTCH; Cartilage; Endochondral; Bone; Development

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APA (6^th Edition):

Kohn, A. (2013). The Role of RBPjκ-Dependent Notch Signaling During Cartilage Development of the Limb Skeleton. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/27308

Chicago Manual of Style (16^th Edition):

Kohn, Anat. “The Role of RBPjκ-Dependent Notch Signaling During Cartilage Development of the Limb Skeleton.” 2013. Doctoral Dissertation, University of Rochester. Accessed January 23, 2020. http://hdl.handle.net/1802/27308.

MLA Handbook (7^th Edition):

Kohn, Anat. “The Role of RBPjκ-Dependent Notch Signaling During Cartilage Development of the Limb Skeleton.” 2013. Web. 23 Jan 2020.

Vancouver:

Kohn A. The Role of RBPjκ-Dependent Notch Signaling During Cartilage Development of the Limb Skeleton. [Internet] [Doctoral dissertation]. University of Rochester; 2013. [cited 2020 Jan 23]. Available from: http://hdl.handle.net/1802/27308.

Council of Science Editors:

Kohn A. The Role of RBPjκ-Dependent Notch Signaling During Cartilage Development of the Limb Skeleton. [Doctoral Dissertation]. University of Rochester; 2013. Available from: http://hdl.handle.net/1802/27308

Montana State University

26. Zignego, Donald Lee. In vitro and in vivo systems mechanobiology of osteoarthritic chondrocytes.

Degree: College of Engineering, 2015, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/10165

► All cells are subjected to and respond to mechanical forces, but the underlying processes linking the mechanical stimuli to biological responses are poorly understood. In… (more)

Subjects/Keywords: Osteoarthritis.; Loads (Mechanics).; Cartilage.

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APA (6^th Edition):

Zignego, D. L. (2015). In vitro and in vivo systems mechanobiology of osteoarthritic chondrocytes. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/10165

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zignego, Donald Lee. “In vitro and in vivo systems mechanobiology of osteoarthritic chondrocytes.” 2015. Thesis, Montana State University. Accessed January 23, 2020. https://scholarworks.montana.edu/xmlui/handle/1/10165.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zignego, Donald Lee. “In vitro and in vivo systems mechanobiology of osteoarthritic chondrocytes.” 2015. Web. 23 Jan 2020.

Vancouver:

Zignego DL. In vitro and in vivo systems mechanobiology of osteoarthritic chondrocytes. [Internet] [Thesis]. Montana State University; 2015. [cited 2020 Jan 23]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/10165.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zignego DL. In vitro and in vivo systems mechanobiology of osteoarthritic chondrocytes. [Thesis]. Montana State University; 2015. Available from: https://scholarworks.montana.edu/xmlui/handle/1/10165

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

27. Cederlund, Anna Angelica. Walking on water : mechanical and material properties of articular cartilage in relation to water content.

Degree: PhD, 2016, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230580 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.693281

► Articular cartilage is a tough and resilient tissue lining the ends of articulating bones. It provides a smooth surface for joint locomotion as well as… (more)

▼ Articular cartilage is a tough and resilient tissue lining the ends of articulating bones. It provides a smooth surface for joint locomotion as well as transmitting the force between bones. The main components of articular cartilage are collagen (20% w/w), proteoglycans (10% w/w) and water (70% w/w). The interactions between these three give the tissue its special characteristics. Water as a molecule is often forgotten when considering the mechanical properties of articular cartilage. This thesis aims to increase our knowledge of the role of water molecules in the load bearing mechanisms of the tissue. It will also investigate the material properties of cartilage as hydrogel. Different rates of loading (impact and slow compression) were used on partially dehydrated articular cartilage (bovine and human). The impact was also recorded using high-speed video cameras. Values of modulus of elasticity, Poisson's ratio, energetic coefficient of restitution were measured together with viscoelastic spectra, by Fourier transformation, and Dynamic Mechanical Analysis. Differential scanning calorimetry (DSC) was also performed on bovine and human articular cartilage, as well as transmission electron microscopy where different freeze substitution solvents were used. The stiffness of the tissue increased and the energetic coefficient of restitution decreased with decreasing water content. Cartilage explants had a smaller volume at the point of full strain than at the start of the impact and this volume loss was associated with the level of hydration of the tissue. Poisson's ratio was not associated with the water content of the tissue. The DSC showed that the water existed in the tissue in different environments, as the exothermic traces showed melting patterns with multiple peaks. Transmission electron micrographs revealed an area surrounding the collagen molecules that could be associated water. These results indicate that water might exist in a structured way in the tissue, and that it is important for the mechanical capabilities of the tissue.

Subjects/Keywords: 612.7; Articular cartilage; Water

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APA (6^th Edition):

Cederlund, A. A. (2016). Walking on water : mechanical and material properties of articular cartilage in relation to water content. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230580 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.693281

Chicago Manual of Style (16^th Edition):

Cederlund, Anna Angelica. “Walking on water : mechanical and material properties of articular cartilage in relation to water content.” 2016. Doctoral Dissertation, University of Aberdeen. Accessed January 23, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230580 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.693281.

MLA Handbook (7^th Edition):

Cederlund, Anna Angelica. “Walking on water : mechanical and material properties of articular cartilage in relation to water content.” 2016. Web. 23 Jan 2020.

Vancouver:

Cederlund AA. Walking on water : mechanical and material properties of articular cartilage in relation to water content. [Internet] [Doctoral dissertation]. University of Aberdeen; 2016. [cited 2020 Jan 23]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230580 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.693281.

Council of Science Editors:

Cederlund AA. Walking on water : mechanical and material properties of articular cartilage in relation to water content. [Doctoral Dissertation]. University of Aberdeen; 2016. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230580 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.693281

University of Western Australia

28. Willers, Craig Robert. Matrix-induced autologous chondrocyte implantation for articular cartilage injury : biology, histology and clinical outcomes.

Degree: PhD, 2008, University of Western Australia

URL: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10579&local_base=GEN01-INS01

► [Truncated abstract] Articular cartilage has no vascular, neural, or lymphatic supply, and hence no intrinsic capacity to self-repair following injury. These physiological limitations, combined with… (more)

▼ [Truncated abstract] Articular cartilage has no vascular, neural, or lymphatic supply, and hence no intrinsic capacity to self-repair following injury. These physiological limitations, combined with the inability of local chondrocytes to contribute to the repair process, translate to poor structural and functional outcomes in these troublesome defects, and osteoarthritic deterioration with time. Subsequently, many surgical therapies have been trialed to stimulate cartilage repair, but none have produced reliable outcomes. Hence, cartilage repair research has been broadened, with many investigators now focused on cell-based treatment. Smith began a revolution of autologous cell research when in 1965 she isolated chondrocytes from articular cartilage and transplanted them into fresh cartilage nodules (Smith, 1965). Since, new technologies and improved techniques have seen autologous chondrocyte implantation (ACI) widely accepted for use in clinical orthopaedics (Bentley et al., 2003; Brittberg et al., 1994; Grande et al., 1989; Peterson et al., 2002). At present, matrix-induced autologous chondrocyte implantation (MACI) is the most surgically simple form of ACI, boasting clinical outcomes comparable to any technique on the market, and far less complications compared to the first generation of ACI - periosteal ACI (Bartlett et al., 2005; Behrens et al., 2006; Gigante et al., 2006; Henderson et al., 2004; Marlovits et al., 2005; Minas, 2001; Willers et al., 2007; Zheng et al., 2007). But whilst MACI has been adopted by the orthopaedic surgeon for articular cartilage repair, many of the molecular, histological, and clinical factors governing patient outcomes are still largely understudied. Firstly we assessed the bioactivity of fibrin sealant (FS - Tisseel®), a critical component of MACI, on the migration and proliferation of human articular chondrocytes in vitro. We also looked to elucidate the associated molecular mechanisms of thrombin, a key active ingredient in FS, by examining the expression and activation of proteaseactivated receptors (PARs), established thrombin receptors. All four PAR isoforms were detected in human chondrocytes, with PAR-1 being the major isoform expressed. '...' This thesis has demonstrated biological, histological, and clinical features of the MACI technique. Our in vitro has supported the use of fibrin sealant and collagen membrane as the major material components of MACI, illustrating improved chondrocyte proliferation, migration, and chondrogenic differentiation. We have evidenced that MACI stimulates successful production of hyaline-like cartilage by 6 months, while also showing that revised and clinically failed repair tissues are predominantly hyaline-like and fibrocartilage with inferior composition. Clinically, we have documented significant improvements in patient repair structure, function, symptoms, quality of life, and satisfaction, whilst concurrently confirming sentiment within the literature regarding the importance of exercise/ rehabilitation for maximising MACI outcome. In…

Subjects/Keywords: Cartilage; Cartilage; Cartilage cells; Osteochondrosis; Matrix-induced autologous chondrocyte implantation; Cartilage repair; Biology; Histology; Clinical outcomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Willers, C. R. (2008). Matrix-induced autologous chondrocyte implantation for articular cartilage injury : biology, histology and clinical outcomes. (Doctoral Dissertation). University of Western Australia. Retrieved from http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10579&local_base=GEN01-INS01

Chicago Manual of Style (16^th Edition):

Willers, Craig Robert. “Matrix-induced autologous chondrocyte implantation for articular cartilage injury : biology, histology and clinical outcomes.” 2008. Doctoral Dissertation, University of Western Australia. Accessed January 23, 2020. http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10579&local_base=GEN01-INS01.

MLA Handbook (7^th Edition):

Willers, Craig Robert. “Matrix-induced autologous chondrocyte implantation for articular cartilage injury : biology, histology and clinical outcomes.” 2008. Web. 23 Jan 2020.

Vancouver:

Willers CR. Matrix-induced autologous chondrocyte implantation for articular cartilage injury : biology, histology and clinical outcomes. [Internet] [Doctoral dissertation]. University of Western Australia; 2008. [cited 2020 Jan 23]. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10579&local_base=GEN01-INS01.

Council of Science Editors:

Willers CR. Matrix-induced autologous chondrocyte implantation for articular cartilage injury : biology, histology and clinical outcomes. [Doctoral Dissertation]. University of Western Australia; 2008. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=10579&local_base=GEN01-INS01

Universiteit Utrecht

29. Goversen, B. Mechanics in articular cartilage regeneration.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/307805

► Articular cartilage is an avascular load-bearing tissue lining the surface of long bones where it serves for the absorbance of shocks as well as the… (more)

Subjects/Keywords: Cartilage; tissue engineering; mechanics; biofabrication

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APA (6^th Edition):

Goversen, B. (2015). Mechanics in articular cartilage regeneration. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/307805

Chicago Manual of Style (16^th Edition):

Goversen, B. “Mechanics in articular cartilage regeneration.” 2015. Masters Thesis, Universiteit Utrecht. Accessed January 23, 2020. http://dspace.library.uu.nl:8080/handle/1874/307805.

MLA Handbook (7^th Edition):

Goversen, B. “Mechanics in articular cartilage regeneration.” 2015. Web. 23 Jan 2020.

Vancouver:

Goversen B. Mechanics in articular cartilage regeneration. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2020 Jan 23]. Available from: http://dspace.library.uu.nl:8080/handle/1874/307805.

Council of Science Editors:

Goversen B. Mechanics in articular cartilage regeneration. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/307805

30. 김, 룡호. Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair.

Degree: 2009, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/1397 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009741

►

" Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair Bone marrow stimulation (BMS) has been used as a… (more)

▼

" Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair Bone marrow stimulation (BMS) has been used as a popular operating technique to repair defect of knee articular cartilage. However, the regenerated tissue from BMS is known as a fibrocartilage rather than a hyaline cartilage and thinner than native cartilage. The objective of this study was to investigate the feasibility of autologous bone marrow-derived buffy coat transplantation in the regeneration of large full-thickness cartilage defect. Rabbits were divided into four groups: the defect was remained untreated as a negative control (Group 1), performed with BMS only using a drill (diameter: 5 mm) and a 17 gauge needle (Group 2), injected with concentrated autologous buffy coat isolated from the iliac crest after BMS (Group 3) and implanted with autologous osteochondral graft (AOG) as a positive control (Group 4). The cartilage defect was finally covered with a thin membrane made of cartilage matrix material using cross suture method in groups 1, 2 and 3. Bone marrow aspirated from iliac crest was diluted with phosphate-buffered saline. Buffy coat was isolated by centrifugation through a Ficoll density gradient at 2000 rpm for 30 min. The repaired cartilages in each experimental group were evaluated by macroscopic observation, histology with Safranin-O staining, immunohistochemistry for collagen type IIand ICRS scoring at 4 and 8 weeks post-operation. In the results of gross observation and Safanin-O staining, the fibrous/hyaline cartilage was regenerated in groups 1 and 2, while the hyaline cartilage tissues with mature matrix and columnar organization of chondrocytes were observed in groups 3 and 4. In the immunohistochemical staining, expression of type II collagen was gradually increased along with time at the pericellular region in the repaired tissues of groups 2, 3 and 4. In the total ICRS, histological score increased significantly along with time in all groups. The ICRS score was higher in groups 3 and 4 than in other groups. This study demonstrated that injection of autologous bone marrow buffy coat after BMS effectively regenerated cartilage defect in rabbit model, being more effective than the BMS alone and similar to AOG. It is speculated that the buffy coat could probably provide more mesenchymal stem cells to regenerate the articular cartilage defect. In addition the extracellular matrix membrane used to cover the defect efficiently prevented leakage of injected cells and blood clots from cartilage defect into the knee joint cavity. In conclusion, the injection of autologous bone marrow-derived buffy coat in case of BMS arthroplasty could be a useful clinical protocol for cartilage repair. "

"TABLE OF CONTENTS ABSTRACT ⅰ TABLE OF CONTENTS ⅲ LIST OF FIGURES ⅳ LIST OF TABLES ⅴ LIST OF ABBREVIATIONS ⅵ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 4 A. Experimental design and surgery 4 B. Macroscopic and histological evaluation 7 C. Histological scoring (ICRS score) 7 …

Advisors/Committee Members: 200724419, 김, 룡호.

Subjects/Keywords: Cartilage; Regeneration; Bone marrow stimulation

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APA (6^th Edition):

김, �. (2009). Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/1397 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009741

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

김, 룡호. “Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair.” 2009. Thesis, Ajou University. Accessed January 23, 2020. http://repository.ajou.ac.kr/handle/201003/1397 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009741.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

김, 룡호. “Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair.” 2009. Web. 23 Jan 2020.

Vancouver:

김 �. Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair. [Internet] [Thesis]. Ajou University; 2009. [cited 2020 Jan 23]. Available from: http://repository.ajou.ac.kr/handle/201003/1397 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009741.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

김 �. Bone Marrow-Derived Buffy Coat Can Supplement the Bone Marrow Stimulation Technique for Articular Cartilage Repair. [Thesis]. Ajou University; 2009. Available from: http://repository.ajou.ac.kr/handle/201003/1397 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009741

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations