subject:(Carbonic Anhydrases isolation amp)

University of Hawaii – Manoa

1. Pal, Archana. Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala.

Degree: 2016, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100737

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Ph.D. University of Hawaii at Manoa 2013.

Carbonic anhydrases (CAs) catalyze rapid interconversion of CO2 and water to bicarbonate and protons. Carbonic anhydrases (CAs) have… (more)

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Ph.D. University of Hawaii at Manoa 2013.

Carbonic anhydrases (CAs) catalyze rapid interconversion of CO2 and water to bicarbonate and protons. Carbonic anhydrases (CAs) have been extensively studied and are known to be involved in various physiological processes such as respiration, cell growth and acid/base regulation in animals, and carbon fixation and photosynthesis in plants, algae and cyanobacteria. Although the beta-type carbonic anhydrases (β-CAs) are crucial for carbon fixation in C4 plants, algae, and cyanobacteria, the role of β-CA in C3 plants is not well-studied. Therefore, the specific objective of this research is to isolate and characterize the genes encoding chloroplastic and cytoplasmic β-CAs from the C3 plant, Leucaena leucocephala (leucaena) and investigate their possible role in the plant by overexpressing the leucaena β-CA in Nicotiana tabacum and also to study the expression pattern of leucaena β-CAs under various physiological stress conditions that affect photosynthesis potential in plants. In this study, two cDNA clones, cacp and cacyt, encoding chloroplastic and cytoplasmic forms of β-CA, respectively, were isolated from a leucaena cDNA library. These clones were further extended to full-length by 5'-and 3'-rapid amplification of cDNA ends (RACE). Deduced amino acid sequences of cacp and cacyt exhibited 79 % homology with each other. To determine the possible role of these genes in leucaena under physiological stresses, quantitative PCR (qPCR) was performed from different tissues of leucaena grown under normal, drought, saline, light, bright light, and dark conditions. The expression of cacp and cacyt was found to be up regulated under drought, salt, and light conditions and down regulated under dark conditions. Bright light conditions, compared with normal light condition, did not exhibit much change in the transcript levels of cacp and cacyt. Additionally, tobacco plants were transformed with leucaena's cacp and cacyt separately and also together resulting in three types of transgenic tobacco plants (i) with cacp only (ii) with cacyt only and (iii) with both cacp and cacyt. These transgenic tobaccos were also studied under stress conditions. This study will give us a deeper insight to understand the role of chloroplastic and cytoplasmic β-CAs in leucaena and also in C3 plants in general.

Subjects/Keywords: Carbonic anhydrases; CAs

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APA (6^th Edition):

Pal, A. (2016). Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100737

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pal, Archana. “Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala.” 2016. Thesis, University of Hawaii – Manoa. Accessed January 19, 2021. http://hdl.handle.net/10125/100737.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pal, Archana. “Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala.” 2016. Web. 19 Jan 2021.

Vancouver:

Pal A. Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10125/100737.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pal A. Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/100737

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

2. Hurt, Jonathan Dale, 1967-. Isolation and characterization of murine carbonic anydrase IV.

Degree: 1996, University of Florida

URL: https://ufdc.ufl.edu/AA00052899

Subjects/Keywords: Amino acids; Complementary DNA; DNA; Enzymes; Exons; Genomics; Imidazoles; Mice; Proteins; Protons; Amino Acid Sequence ( mesh ); Base Sequence ( mesh ); Carbonic Anhydrases – analysis ( mesh ); Carbonic Anhydrases – genetics ( mesh ); Carbonic Anhydrases – isolation &; purification ( mesh ); Carbonic Anhydrases – pharmacokinetics ( mesh ); Chromosome Mapping ( mesh ); Department of Biochemistry and Molecular Biology thesis Ph.D ( mesh ); Molecular Sequence Data ( mesh ); Protein Folding ( mesh ); Research ( mesh )

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APA (6^th Edition):

Hurt, Jonathan Dale, 1. (1996). Isolation and characterization of murine carbonic anydrase IV. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00052899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hurt, Jonathan Dale, 1967-. “Isolation and characterization of murine carbonic anydrase IV.” 1996. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00052899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hurt, Jonathan Dale, 1967-. “Isolation and characterization of murine carbonic anydrase IV.” 1996. Web. 19 Jan 2021.

Vancouver:

Hurt, Jonathan Dale 1. Isolation and characterization of murine carbonic anydrase IV. [Internet] [Thesis]. University of Florida; 1996. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00052899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hurt, Jonathan Dale 1. Isolation and characterization of murine carbonic anydrase IV. [Thesis]. University of Florida; 1996. Available from: https://ufdc.ufl.edu/AA00052899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tampere University

3. Yrjänäinen, Alma. Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi .

Degree: 2019, Tampere University

URL: https://trepo.tuni.fi/handle/10024/105797

► Background and aims of study: Carbonic anhydrase VI, CA VI, belongs to carbonic anhydrase isoenzymes that is known to secrete from serous acinar cells in… (more)

Subjects/Keywords: carbonic anhydrases; carbonic anhydrase VI; size-exclusion chromatography; static light scattering; dynamic light scattering

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APA (6^th Edition):

Yrjänäinen, A. (2019). Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi . (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/105797

Chicago Manual of Style (16^th Edition):

Yrjänäinen, Alma. “Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi .” 2019. Masters Thesis, Tampere University. Accessed January 19, 2021. https://trepo.tuni.fi/handle/10024/105797.

MLA Handbook (7^th Edition):

Yrjänäinen, Alma. “Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi .” 2019. Web. 19 Jan 2021.

Vancouver:

Yrjänäinen A. Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi . [Internet] [Masters thesis]. Tampere University; 2019. [cited 2021 Jan 19]. Available from: https://trepo.tuni.fi/handle/10024/105797.

Council of Science Editors:

Yrjänäinen A. Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi . [Masters Thesis]. Tampere University; 2019. Available from: https://trepo.tuni.fi/handle/10024/105797

Universidade Estadual de Campinas

4. Picco, Daniele de Cassia Rodrigues, 1981-. Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522

► Abstract: The carbonic anhydrase VI (CA VI) is the main enzyme responsible for maintaining the buffer capacity in the oral environment. There are no reports… (more)

▼ Abstract: The carbonic anhydrase VI (CA VI) is the main enzyme responsible for maintaining the buffer capacity in the oral environment. There are no reports in the literature on the relationship between the activity of this isoenzyme, pH, flow and buffering capacity and the prevalence of caries, and if the activity of CA VI can be employed as caries risk indicator. The objectives of this thesis were: 1) Conduct a systematic review of the literature 2) Determine the concentration and activity of CA VI in saliva and dental plaque of children with caries (C) and caries-free (CF) and the salivary flow, pH and buffering capacity; and 3) To investigate the relationship between these variables in saliva and dental plaque and tooth decay. For this, 74 students aged 7 to 9 years old were divided into 2 groups. It performed clinical examination, saliva collection and biofilm. The assays of the concentration of CA VI was performed by Elisa. The analysis of the CA VI activity by zymography. The salivary flow rate was expressed in ml/min. The salivary pH was determined by a portable pHmeter and the plaque pH with a selective electrode. The buffering capacity of saliva was measured eletrometrically. Data were subjected to Student's t test and Mann-Whitney, besides the Pearson and Spearman correlation analysis (? = 0.05). A bivariate analysis was performed in order to verify associations between the dependent and independent variables. In multivariate modeling, associations between the dependent and independent variables were expressed as odds ratios with 95% confidence intervals. The results showed significantly higher values of flow, salivar pH and buffering capacity in the CF group. The pH of the biofilm was also higher in the CF group. The concentration of CA VI in saliva and plaque were significantly higher in the LC group. The activity of CA VI was significantly higher in group C in saliva and plaque. There was a negative correlation between salivary buffering capacity and tooth decay. Moreover, a positive correlation was observed between the concentration and activity of CA VI in saliva and a negative correlation between the buffer capacity and isoenzyme activity in group C. In the CF group there was a negative correlation between pH and concentration of CA VI. A minor salivary flow and increased isoenzyme activity were significantly associated with caries. For dental plaque, it was observed negative correlation between activity and concentration of CA VI and between pH and activity of CA VI in the LC group. In the C group there was a negative correlation between pH and concentration of the CA VI. It concludes that the CA VI proved to be more active in saliva and dental plaque of children with active decay. Greater concentration of CA VI in saliva and dental plaque of caries-free children suggests that they are less exposed to cariogenic challenges, the system carbonate would be less active. The CA VI in saliva and dental plaque can be considered an anti-caries protein, as well as a biomarker for dental caries Advisors/Committee Members: amp;pagesize-30">UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), amp;pagesize-30">Nobre dos Santos, Marinês, 1956- (advisor), amp;pagesize-30">Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), amp;pagesize-30">Programa de Pós-Graduação em Odontologia (nameofprogram), amp;pagesize-30">Rodrigues, Lidiany Karla Azevedo (committee member), amp;pagesize-30">Pessan, Juliano Pelim (committee member), amp;pagesize-30">Silva, Alan Roger dos Santos (committee member), amp;pagesize-30">Alarcon, Carolina Steiner Oliveira (committee member).

Subjects/Keywords: Anidrases carbônicas; Saliva; Placas dentárias; Cárie dentária; Carbonic anhydrases; Saliva; Dental plaque; Dental caries

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APA (6^th Edition):

Picco, Daniele de Cassia Rodrigues, 1. (2016). Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Picco, Daniele de Cassia Rodrigues, 1981-. “Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 19, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Picco, Daniele de Cassia Rodrigues, 1981-. “Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay.” 2016. Web. 19 Jan 2021.

Vancouver:

Picco, Daniele de Cassia Rodrigues 1. Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 19]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Picco, Daniele de Cassia Rodrigues 1. Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

5. Souza, Thayse Rodrigues de, 1987-. Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028

► Abstract: The carbonic anhydrase VI (CAVI) and ?-amylase (SAA) enzymes are present in saliva. AC VI is responsible for catalyzing the main reaction buffering the… (more)

▼ Abstract: The carbonic anhydrase VI (CAVI) and ?-amylase (SAA) enzymes are present in saliva. AC VI is responsible for catalyzing the main reaction buffering the oral cavity. SAA is associated with the pellicle and biofilms formation and starch metabolism. There are no reports in the literature that have longitudinally investigated the relationship between AC VI and dental caries and a cross-sectional study to investigate the SAA activity after a cariogenic challenge. The objectives of the Chapter 1 of this thesis were: Determine the stimulated salivary flow (SSFR), buffer capacity (BC) and CA VI activity in the saliva of children with caries and caries-free before and after rinsing with a sucrose solution to 20% and to investigate the relationship of these variables with dental caries in a longitudinal study of one year of follow-up. And of the Chapter 2: Investigate the SAA activity in saliva of children with caries and caries-free before and after rinsing with a sucrose solution at 20% and its relationship with SSFR, BC and dental caries in a cross-sectional study. Were allocated to the study of Chapter 1 47 children 48-78 months age, divided into three groups after calculation of caries increment after one year: caries free group (CF), caries lesion group (CL) and arrestment caries group (AC). And in Chapter 2, 38 children aging 48-77 months old, divided into two groups: caries lesion group (CL) and caries free group (CF). The activity of CA VI was quantified by zymography. The SSFR was expressed in mL/min. The BC was measured by Ericsson¿s method. The SAA activity was analyzed by the enzyme kinetic assay. Wilcoxon test and the Kruskal-Wallis test for paired comparisons of the values of CAVI before and after the rinses and comparison between groups respectively. To SSFR and BC data were employed the tests mentioned above in the two Chapters. The Student t test paired and independent were employed to the SAA data. It was also performed Spearman correlation analysis (? = 0.05). The results of chapter 1 show that CA VI activity significantly decreased after the cariogenic challenge at the CF group in baseline and follow-up and at AC group only at the follow-up (p= 0.037, p=0.028 e p=0.027, respectively). No change in CA VI activity was found at the two periods of the study in CL group. Salivary CA VI activity before rinse at the baseline shows also a negative correlation with dental caries at the baseline before and after rinse and at the follow-up before the rinse in the CL group (r=-0.609, p=0.004 e r=-0.516, p=0,020, r= -0.545, p=0.013, respectively). A negative correlation was found between dental caries at baseline as well at follow-up and BC after rinse at follow-up (r=-0.345, p=0.017 e r=-0.303, p=0.038 respectively). The results of Chapter 2 shows that the CL group exhibited a significant increase on SAA activity after rinse (p=0.001), and significantly differed from CF group (p=0.033). The results of the two Chapters show a significant increase and decrease of SSFR and BC respectively after the sucrose… Advisors/Committee Members: amp;pagesize-30">UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), amp;pagesize-30">Nobre dos Santos, Marinês, 1956- (advisor), amp;pagesize-30">Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), amp;pagesize-30">Programa de Pós-Graduação em Odontologia (nameofprogram), amp;pagesize-30">Delbem, Alberto Carlos Botazzo (committee member), amp;pagesize-30">Valarelli, Thais Marchini de Oliveira (committee member), amp;pagesize-30">Ferrua, Paula Midori Castelo (committee member), amp;pagesize-30">Leite, Michelle Franz Montan Braga (committee member).

Subjects/Keywords: Anidrases carbônicas; Alfa-amilases; Saliva; Cárie dentária; Carbonic anhydrases; Alpha-amylases; Saliva; Dental caries

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APA (6^th Edition):

Souza, Thayse Rodrigues de, 1. (2016). Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Souza, Thayse Rodrigues de, 1987-. “Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 19, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Souza, Thayse Rodrigues de, 1987-. “Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children.” 2016. Web. 19 Jan 2021.

Vancouver:

Souza, Thayse Rodrigues de 1. Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 19]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Souza, Thayse Rodrigues de 1. Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Le Goff, Carine. Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum.

Degree: Docteur es, Sciences de la vie, 2016, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2016PA066439

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Le processus de calcification chez Corallium rubrum conduit à la formation de deux structures squelettiques composées de CaCO3, l’axe squelettique et les sclérites, de taille… (more)

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Le processus de calcification chez Corallium rubrum conduit à la formation de deux structures squelettiques composées de CaCO3, l’axe squelettique et les sclérites, de taille et de forme différentes. Comme chez de nombreuses espèces calcifiantes, la calcification se fait sous contrôle biologique impliquant notamment des enzymes et des transporteurs ioniques. Une question centrale est d’identifier les mécanismes communs ou propres à chaque espèce qui sous-tendent leur convergence fonctionnelle envers ce processus. Deux approches ont été utilisées pour caractériser ces mécanismes chez C. rubrum: 1) Une approche physiologique avec le développement d’une technique de culture de microcolonies sur lamelles permettant d’observer différents stades de calcification, et de mesurer le pH aux sites de calcification par imagerie confocale ; 2) Une approche moléculaire afin de caractériser une famille d’enzymes, les anhydrases carboniques (ACs), qui jouent un rôle clef dans la calcification.Nous avons réalisé une cartographie du pH en effectuant des mesures dans différents compartiments intra- et extracellulaires. Nos résultats montrent notamment que le pH aux sites de calcification est supérieur à celui du milieu circulant dans les canaux gastrodermiques et non à celui l’eau de mer. Les mesures d’expression différentielle des ACs dans différents tissus mettent en évidence une isozyme préférentiellement exprimée dans les cellules calcifiantes.Ces résultats intégrés dans un contexte de calcification comparée pointent sur la convergence fonctionnelle des ACs et de la régulation du pH par les cellules calcifiantes, tout en soulignant des divergences évolutives.

The calcification process in Corallium rubrum leads to the formation of two skeletal structures made of calcium carbonate, the skeletal axis and sclerites, of different size and shape. As in many calcifying species, calcification occurs under a biological control that involves enzymes and ion transporters. A central issue is to determine the common and the species-specific mechanisms of calcification in order to identify functional convergences in this process. Two approaches were used to characterize these mechanisms in C. rubrum: 1) A physiological approach involving the development of a microcolony culture technique on glass coverslips, allowing the observation of the different stages of calcification, and the measurement of pH at the sites of calcification by the use of confocal microscopy; 2) A molecular approach to characterize an enzyme family, the carbonic anhydrases, which play a key role in calcification.We performed pH mapping by making measurements in different intra- and extracellular compartments. Our results show higher pH values at the sites of calcification compared with the fluid circulating in the gastrodermal canals, but not with the seawater surrounding the microcolony. Measurements of differential expression of carbonic anhydrases in different tissue fractions highlight an isozyme preferentially expressed in the calcifying cells.Within comparative…

Advisors/Committee Members: amp;pagesize-30">Tambutté, Sylvie (thesis director).

Subjects/Keywords: Corail; Corallium rubrum; Octocoralliaire; Calcification; Anhydrase carbonique; PH; Calcification; Corallium rubrum; Carbonic anhydrases; 571.1

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APA (6^th Edition):

Le Goff, C. (2016). Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066439

Chicago Manual of Style (16^th Edition):

Le Goff, Carine. “Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed January 19, 2021. http://www.theses.fr/2016PA066439.

MLA Handbook (7^th Edition):

Le Goff, Carine. “Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum.” 2016. Web. 19 Jan 2021.

Vancouver:

Le Goff C. Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2016PA066439.

Council of Science Editors:

Le Goff C. Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066439

University of Edinburgh

7. Hughes, Sam. Investigating protein structure by top-down native protein mass spectrometry.

Degree: PhD, 2019, University of Edinburgh

URL: http://hdl.handle.net/1842/36627

► Determining protein structure is the central challenge of structural biology, owing to the integral role of proteins in biological systems along with the complexity and… (more)

▼ Determining protein structure is the central challenge of structural biology, owing to the integral role of proteins in biological systems along with the complexity and diversity of their structures. It is not only an important challenge, but a difficult one; the large size of proteins gives rise to a limitless range of potential structures, while the traditional methods of NMR and crystallography both carry specific limitations. Hence, there is a drive to develop new, orthogonal techniques that could provide additional insight into protein structure. Mass spectrometry (MS) is a ubiquitous analytical technique across all fields of chemistry, and the development of "soft" ionisation techniques has allowed noncovalent interactions to be retained in the gas phase and observed by MS. This in turn has led to the development of "native" MS in which proteins can be analysed and studied in the gas phase with their structures largely intact. Top-down MS of native proteins has been suggested as a potential avenue for studying protein structure. Here, the top-down technique of electron capture dissociation (ECD), which does not disrupt noncovalent interactions, is investigated for its potential to observe protein structure in native MS. Firstly, ECD was used to determine the sites at which a range of metal ions bind to the unstructured protein α-synuclein. These results were analysed through both a traditional top-down workflow and a novel mass defect based analysis. This mass defect analysis demonstrated how metal-containing fragments can detected without determination of their monoisotopic signal and shows potential to be applied in a range of top-down experiments. Secondly, ECD was investigated for its ability to probe the structure of native proteins. Carbonic anhydrase was used as a model protein, and it was observed that ECD can be used to monitor gas-phase unfolding pathways in much greater detail than otherwise available. However, studying multiple structural homologues showed that ECD is not exclusively directed by structure, but rather that availability of positive charge plays an equally important role in directing fragmentation. This was further investigated by producing K→A variants of carbonic anhydrase specifically designed to target ECD-sensitive regions of the protein. Our results demonstrate that minor differences to the primary sequence of a protein can significantly alter the observed ECD fragmentation patterns.

Subjects/Keywords: mass spectrometry; protein structure; carbonic anhydrases; electron capture dissociation; ECD; ECD fragmentation patterns

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hughes, S. (2019). Investigating protein structure by top-down native protein mass spectrometry. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/36627

Chicago Manual of Style (16^th Edition):

Hughes, Sam. “Investigating protein structure by top-down native protein mass spectrometry.” 2019. Doctoral Dissertation, University of Edinburgh. Accessed January 19, 2021. http://hdl.handle.net/1842/36627.

MLA Handbook (7^th Edition):

Hughes, Sam. “Investigating protein structure by top-down native protein mass spectrometry.” 2019. Web. 19 Jan 2021.

Vancouver:

Hughes S. Investigating protein structure by top-down native protein mass spectrometry. [Internet] [Doctoral dissertation]. University of Edinburgh; 2019. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1842/36627.

Council of Science Editors:

Hughes S. Investigating protein structure by top-down native protein mass spectrometry. [Doctoral Dissertation]. University of Edinburgh; 2019. Available from: http://hdl.handle.net/1842/36627

University of Oulu

8. Karhumaa, P. (Pepe). Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form.

Degree: 2002, University of Oulu

URL: http://urn.fi/urn:isbn:9514266641

► Abstract Carbonic anhydrases (CAs) are a group of zinc-containing metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate (CO2 + H2O ⇔ HCO3- +… (more)

▼ Abstract Carbonic anhydrases (CAs) are a group of zinc-containing metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate (CO2 + H2O ⇔ HCO3- + H+). They are present in almost all organs and are implicated in various biological functions, the most important of which is participation in the regulation of ion, water, and acid-base balance. Recently, some members of the CA gene family have been suggested to promote cell proliferation and to act as trophic growth factors. The present study was undertaken to examine the distribution of CA isoenzymes in the reproductive system, to attain a more detailed view on their linkage to the reproductive processes and to neonatal development. The expression of membrane-bound CA IX and CA XII was studied in the female and male reproductive tracts by immunohistochemistry and western blotting. CA XII was found to be expressed in the basolateral plasma membrane of luminal and glandular epithelia in human uterus. In human efferent ducts, it was located in the basolateral plasma membrane of luminal epithelium, where it coexpressed with Aquaporin-1. In epididymal duct, CA XII was only expressed in occasional epithelial cells. These cells coexpressed CA II, suggesting that they represent apical mitochondria-rich cells (AMRC). CA IX was also expressed in the basolateral plasma membrane of luminal epithelium in human efferent ducts, but its expression was not uniform among the tubules. These findings suggest that basolateral plasma membrane-associated CA IX and CA XII contribute, along with CA II and CA IV, to the regulation of acid-base balance and water transport in the reproductive tract. Western blotting of rat Leydig tumor cells and testis for CA II revealed an unidentified 66-kDa polypeptide band. The polypeptide was successfully purified from several rat tissues using CA inhibitor affinity chromatography. The amino acid sequence of the polypeptide showed it to be identical to NonO/p54nrb, a non-POU domain-containing octamer-binding protein previously implicated in transcriptional regulation. The recombinant NonO/p54nrb was shown to display CA activity, and the antibody to it predominantly immunostained the nuclei in lymphocytes, where CA activity was also detected histochemically. Accordingly, the nuclear Leydig cell CA immunoreactivity represents NonO/p54nrb. It is classified as a novel, nonclassical CA, and it may participate in pH-related events in the nucleus. Human and rat milk was found to contain CA VI by immunohistochemistry and western blotting. The enzyme purified from human milk by CA inhibitor affinity chromatography was confirmed by PNGase F digestion and amino acid sequence as CA VI. The CA VI concentrations in human colostral milk were approximately eight times higher than those in mature milk (34.7 mg/l vs. 4.5 mg/l). Secretion of CA VI into milk is suggested by its localization in the alveolar epithelium of the rat mammary gland. The structural and functional stability of CA VI in an acidic milieu, its suggested growth-supporting…

Subjects/Keywords: carbonic anhydrases; efferent ducts; milk; testis; uterus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Karhumaa, P. (. (2002). Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9514266641

Chicago Manual of Style (16^th Edition):

Karhumaa, P (Pepe). “Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form.” 2002. Doctoral Dissertation, University of Oulu. Accessed January 19, 2021. http://urn.fi/urn:isbn:9514266641.

MLA Handbook (7^th Edition):

Karhumaa, P (Pepe). “Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form.” 2002. Web. 19 Jan 2021.

Vancouver:

Karhumaa P(. Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form. [Internet] [Doctoral dissertation]. University of Oulu; 2002. [cited 2021 Jan 19]. Available from: http://urn.fi/urn:isbn:9514266641.

Council of Science Editors:

Karhumaa P(. Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form. [Doctoral Dissertation]. University of Oulu; 2002. Available from: http://urn.fi/urn:isbn:9514266641

Wayne State University

9. Alsaab, Hashem Obaid. Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma.

Degree: PhD, Pharmaceutical Sciences, 2018, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/2002

► Renal Cell Carcinoma (RCC) contributes to more than 90% of the most common form of kidney tumor and remains one of the ten leading… (more)

▼ Renal Cell Carcinoma (RCC) contributes to more than 90% of the most common form of kidney tumor and remains one of the ten leading causes of cancer death in the United States. Although surgery remains an option for operable tumors, high metastatic index and resistance to radiation and chemotherapies prompted recent development of therapeutics that target the RCC angiogenesis and cell proliferation pathways. Tyrosine kinase inhibitors or TKIs (Cabozantinib, Axitinib, Sorafenib, and Sunitinib) and mammalian target of rapamycin (mTOR) inhibitors (Temsirolimus and Everolimus) have increased the therapeutic options for treating RCC. Although the impact towards decreasing disease progression is encouraging, a substantial proportion of patients do not respond adequately, and therapy resistance almost inevitably occurs. Eventually, new strategies with encouraging results have emerged that include immunotherapy, such as the programmed death-1 inhibitor (Nivolumab), cytokines, and a combination of chemo-immune therapy. Nevertheless, the 5-year disease free progression remains a meager <10% and these medicines produce fetal toxicity, contributing to poor overall prognosis. Thus, developing alternative strategies with effective treatment options remains an urgent unmet need for therapy-resistant RCC. In this regard, combination treatment targeting different cancer survival pathway of tumor microenvironment can be advantageous. Along these lines, we have pursued different combination drug regimens, including inhibitors that target mTOR (everolimus) and RTK or VEGFR (cabozantinib or sorafenib), as possible treatment strategies. In addition, we explored the rational design of nanoparticles to selectively deliver a variety of therapeutic payloads to target hypoxic tumor microenvironment overexpressing carbonic anhydrase-IX (CAIX). Our strategy involved establishing a library of tumor penetrating nanocarriers carrying combination drug payload of RTK-inhibitor with our own apoptosis inducer/CARP-1 protein functional mimetics (CFM-4.16). Nano-carriers were tailored to have varying composition and size that could: (i) efficiently reach the tumor core; (ii) target tumor multi-components including cancer epithelial cells and tumor-associated macrophages (TAM) for overcoming drug resistance in RCC. Specifically, the current work was focused on multimodal approaches, including (a) Optimization of hypoxia marker (CA IX) targeted polymer-lipid nano-formulation (PLNP) using copper-free ‘click’ chemistry; (b) In vitro and in vivo pre-clinical imaging and therapy of PLNP loaded with multiple drugs in inhibiting RCCs using mice bearing resistant RCCs and human cancer mimicking patient-derived xenografts (PDx). The results of antitumor efficacy and biodistribution of targeted PLNPs in animals bearing RCC xenograft and PDx models revealed selective accumulation of drugs at tumor sites resulting in greater tumor growth inhibition with reduced side effects. The highlights of the targeted formulation include specific tumor uptake,… Advisors/Committee Members: amp;pagesize-30">Arun K. Iyer.

Subjects/Keywords: angiogenesis; Carbonic Anhydrases IX; Hypoxia Targeting; Nanoformulation; Renal Cell Carcinoma; Tyrosine kinase inhibitors; Medicinal Chemistry and Pharmaceutics

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APA (6^th Edition):

Alsaab, H. O. (2018). Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/2002

Chicago Manual of Style (16^th Edition):

Alsaab, Hashem Obaid. “Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma.” 2018. Doctoral Dissertation, Wayne State University. Accessed January 19, 2021. https://digitalcommons.wayne.edu/oa_dissertations/2002.

MLA Handbook (7^th Edition):

Alsaab, Hashem Obaid. “Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma.” 2018. Web. 19 Jan 2021.

Vancouver:

Alsaab HO. Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma. [Internet] [Doctoral dissertation]. Wayne State University; 2018. [cited 2021 Jan 19]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/2002.

Council of Science Editors:

Alsaab HO. Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma. [Doctoral Dissertation]. Wayne State University; 2018. Available from: https://digitalcommons.wayne.edu/oa_dissertations/2002

Tampere University

10. Isokangas, Lydia. Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases .

Degree: 2016, Tampere University

URL: https://trepo.tuni.fi/handle/10024/99277

► Background and Aims Humans are able to quickly recognize and evaluate visual patterns, thus this thesis aims to apply this feature to the analysis of… (more)

▼ Background and Aims Humans are able to quickly recognize and evaluate visual patterns, thus this thesis aims to apply this feature to the analysis of aspects of the conservation of carbonic anhydrase proteins. This was facilitated through the creation of two pipelines: * One to create a publically available specialized database to service the CA research world named CAbase, and, * One to create a visual display of the aligned exons of the cDNA transcripts contained within CAbase with indicators to show the positions of start and stop codons along with the locations of the predicted signal and mitochondrial targeting peptides. This pipeline was named Exon_Analysis. Carbonic anhydrases (CAs) are ubiquitous proteins that reversibly catalyse carbon dioxide into carbonic acid. Through the events of duplication, the CAs exist in at least 16 different isoforms and potentially up to 17 different isoforms. Methods The pipelines were created using freely available tools that included python, MySQL, various bioinformatic tools such as Clustal Omega, PRANK, BLAST and Pal2Nal. The data for CAbase was extracted from Ensembl, NCBI, UniProt, RSCB PDB, UniGene and FlyBase. Additionally, calculated data from using SignalP and TargetP was also included. CAbase is hosted on the Amazon Web Server and can be accessed using any computer that has access to the Internet and has MySQL installed. Exon_Analysis draws a scaled exon MSA schematic based on a PRANK MSA of the cDNA transcripts for a CA isoform. The exons and other indicators such as the start and stop codon, and the signal and target peptides are all drawn in different colours and in their scaled locations. Thus it is possible to see the conserved nature of the exons within the coding regions and the aligned start and stop codons and the peptides for each CA isoform. Results CAbase is now publically available for anyone to use. However, it is still somewhat user unfriendly due to the requirement that user be familiar with SQL. CAbase facilitated the use of Exon_Analysis. This pipeline has enabled the quality control of the predicted genes one exon at a time. Evolutionary events such as the conservation of short exons within CAs VI, IX, XII, XIV, X and XI has been shown in the exon MSA schematics. Both tools could be adapted for use with other proteins. Conclusion CAbase is a specialized database for CA researchers that can continue to be adapted to their needs. It allows researchers to create specific queries without having to filter for unwanted proteins. Exon_Analysis creates an exon MSA schematic to visualise the relationship between the exons and other features of interest. This facilitates the quality control of predicted genes one exon at a time.

Subjects/Keywords: carbonic anhydrases; specialised database; exon; ca; predicted genes quality check; signal peptide; mitochondrial targeting; exon analysis pipeline; visual assessment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Isokangas, L. (2016). Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases . (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/99277

Chicago Manual of Style (16^th Edition):

Isokangas, Lydia. “Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases .” 2016. Masters Thesis, Tampere University. Accessed January 19, 2021. https://trepo.tuni.fi/handle/10024/99277.

MLA Handbook (7^th Edition):

Isokangas, Lydia. “Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases .” 2016. Web. 19 Jan 2021.

Vancouver:

Isokangas L. Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases . [Internet] [Masters thesis]. Tampere University; 2016. [cited 2021 Jan 19]. Available from: https://trepo.tuni.fi/handle/10024/99277.

Council of Science Editors:

Isokangas L. Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases . [Masters Thesis]. Tampere University; 2016. Available from: https://trepo.tuni.fi/handle/10024/99277

11. Canela, Heliara Maria Spina. Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus.

Degree: Mestrado, Biociências Aplicadas à Farmácia, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;

► O fungo Aspergillus fumigatus é o segundo maior causador de infecções fúngicas invasivas em pacientes imunocomprometidos e a principal espécie causadora da aspergilose invasiva, doença… (more)

▼ O fungo Aspergillus fumigatus é o segundo maior causador de infecções fúngicas invasivas em pacientes imunocomprometidos e a principal espécie causadora da aspergilose invasiva, doença de alta taxa de mortalidade que atinge principalmente os pulmões e que pode se disseminar pelo organismo. Durante o processo de infecção, o fungo precisa adaptar-se ao organismo do hospedeiro e um dos obstáculos encontrados é a mudança na concentração de dióxido de carbono (CO2), que, de 0,033% no ambiente, chega a até 6% no interior do hospedeiro. As anidrases carbônicas são enzimas envolvidas na hidratação reversível do CO2 e já foram apontadas como importantes na virulência de patógenos como Plasmodium falciparum, Mycobacterium tuberculosis, Helicobacter pylori, Cryptococcus neoformans e Candida albicans. Esse trabalho teve como objetivo avaliar o papel da enzima anidrase carbônica no desenvolvimento e virulência do fungo A. fumigatus, que apresenta quatro homólogos desta enzima (cafA, cafB, cafC e cafD). Para isso, foram utilizadas linhagens de A. fumigatus com os homólogos da enzima deletados (?cafA, ?cafB, ?cafC, ?cafD e ?cafA?cafB) e a linhagem selvagem (?akuBku80), da qual foram originadas as mutantes. Foram realizadas avaliações fenotípicas da estrutura dos conidióforos das diferentes linhagens, determinação da sensibilidade frente a diferentes agentes estressantes (antifúngicos, promotores de apoptose, estresse iônico, nitroativo, oxidativo, e de parede celular) e determinação da expressão gênica global em diferentes concentrações de CO2. Foi verificado que a deleção de cada um dos homólogos da anidrase carbônica de A. fumigatus não interfere na estrutura dos conidióforos deste fungo. Por outro lado, a deleção induziu alteração da sensibilidade do fungo frente a alguns compostos estressantes (ácido acético e peróxido de hidrogênio). Ainda, a análise da expressão gênica revelou um gene envolvido na adaptação do fungo ao aumento da concentração de CO2, o gene cipC, que não apresenta homólogos nas células de mamíferos. Este gene foi caracterizado neste trabalho por meio de sua deleção na linhagem selvagem (?akuBku80) de A. fumigatus e avaliação fenotípica microscópica e de sensibilidade a agentes estressantes (antifúngicos, promotores de apoptose, estresse iônico, nitroativo, oxidativo, e de parede celular). A deleção do gene não interferiu na estrutura do fungo, porém aumentou sua sensibilidade a alguns compostos (calcoflúor e menadiona). Foram realizados, ainda, testes de virulência em modelo animal utilizando-se o mutante ?cipC, os quais revelaram que a deleção deste gene atenua a virulência do fungo. Assim, foi possível concluir que as anidrases carbônicas não são relevantes para o desenvolvimento e virulência de A. fumigatus; porém, este fungo modifica a expressão de seus genes de modo a adaptar-se às variações na concentração atmosférica de CO2. O gene cipC está envolvido nesse processo de adaptação e é importante para o desenvolvimento do fungo e sua virulência, tornando-se um alvo para o estudo de novas terapias para o… Advisors/Committee Members: amp;pagesize-30">Balieiro, Marcia Eliana da Silva Ferreira.

Subjects/Keywords: Análise da expressão gênica; Análise da virulência; Anidrases carbônicas; Aspergillus fumigatus; Aspergillus fumigatus; Aspergilose invasiva; Carbonic anhydrases; cipC; cipC; Genic expression analysis; Invasive aspergillosis; Virulence analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Canela, H. M. S. (2013). Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;

Chicago Manual of Style (16^th Edition):

Canela, Heliara Maria Spina. “Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus.” 2013. Masters Thesis, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;.

MLA Handbook (7^th Edition):

Canela, Heliara Maria Spina. “Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus.” 2013. Web. 19 Jan 2021.

Vancouver:

Canela HMS. Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;.

Council of Science Editors:

Canela HMS. Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;

Louisiana State University

12. Ynalvez, Ruby Agno. Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii.

Degree: PhD, 2007, Louisiana State University

URL: etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778

► Aquatic photosynthetic organisms such as the green alga, C. reinhardtii respond to low carbon dioxide conditions by inducing a carbon dioxide concentrating mechanism (CCM). Important… (more)

Subjects/Keywords: carbon dioxide concentrating mechanism; carbonic anhydrases; insertional mutant; Chlamydomonas reinhardtii

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APA (6^th Edition):

Ynalvez, R. A. (2007). Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778

Chicago Manual of Style (16^th Edition):

Ynalvez, Ruby Agno. “Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii.” 2007. Doctoral Dissertation, Louisiana State University. Accessed January 19, 2021. etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778.

MLA Handbook (7^th Edition):

Ynalvez, Ruby Agno. “Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii.” 2007. Web. 19 Jan 2021.

Vancouver:

Ynalvez RA. Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Louisiana State University; 2007. [cited 2021 Jan 19]. Available from: etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778.

Council of Science Editors:

Ynalvez RA. Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Louisiana State University; 2007. Available from: etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778

University of Florida

13. Feldstein, Judith Breiner, 1952-. Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat.

Degree: 1983, University of Florida

URL: https://ufdc.ufl.edu/AA00052894

Subjects/Keywords: Acinar cells; Bicarbonates; Enzymes; Erythrocytes; Gels; Kinetics; Molecular weight; pH; Rats; Saliva; Carbonic Anhydrases ( mesh ); Carbonic Anhydrases – analysis ( mesh ); Pharmacology and Therapeutics thesis Ph.D ( mesh ); Saliva – secretion ( mesh ); Salivary Glands ( mesh )

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APA (6^th Edition):

Feldstein, Judith Breiner, 1. (1983). Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00052894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Feldstein, Judith Breiner, 1952-. “Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat.” 1983. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00052894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Feldstein, Judith Breiner, 1952-. “Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat.” 1983. Web. 19 Jan 2021.

Vancouver:

Feldstein, Judith Breiner 1. Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat. [Internet] [Thesis]. University of Florida; 1983. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00052894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Feldstein, Judith Breiner 1. Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat. [Thesis]. University of Florida; 1983. Available from: https://ufdc.ufl.edu/AA00052894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

14. Wallace, David Brian. Genomic characterisation of Southern African isolates of capripox and avipox viruses.

Degree: Image, Medicine, 1994, University of Cape Town

URL: http://hdl.handle.net/11427/25832

Subjects/Keywords: Avipoxvirus - isolation &; purification; Capripoxvirus - isolation &; purification; Genome, Viral - Africa, Southern

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APA (6^th Edition):

Wallace, D. B. (1994). Genomic characterisation of Southern African isolates of capripox and avipox viruses. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/25832

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wallace, David Brian. “Genomic characterisation of Southern African isolates of capripox and avipox viruses.” 1994. Thesis, University of Cape Town. Accessed January 19, 2021. http://hdl.handle.net/11427/25832.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wallace, David Brian. “Genomic characterisation of Southern African isolates of capripox and avipox viruses.” 1994. Web. 19 Jan 2021.

Vancouver:

Wallace DB. Genomic characterisation of Southern African isolates of capripox and avipox viruses. [Internet] [Thesis]. University of Cape Town; 1994. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11427/25832.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wallace DB. Genomic characterisation of Southern African isolates of capripox and avipox viruses. [Thesis]. University of Cape Town; 1994. Available from: http://hdl.handle.net/11427/25832

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

15. Kararli, Tugrul T., 1956-. Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II.

Degree: 1984, University of Florida

URL: https://ufdc.ufl.edu/AA00004893

Subjects/Keywords: Active sites; Anions; Catalysis; Enzymes; Fluorescence; Imidazoles; Kinetics; pH; Protons; Sulfonamides; Carbonic Anhydrases ( mesh ); Carbonic anhydrase inhibitors ( mesh ); Pharmacology and Therapeutics thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kararli, Tugrul T., 1. (1984). Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00004893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kararli, Tugrul T., 1956-. “Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II.” 1984. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00004893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kararli, Tugrul T., 1956-. “Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II.” 1984. Web. 19 Jan 2021.

Vancouver:

Kararli, Tugrul T. 1. Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II. [Internet] [Thesis]. University of Florida; 1984. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00004893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kararli, Tugrul T. 1. Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II. [Thesis]. University of Florida; 1984. Available from: https://ufdc.ufl.edu/AA00004893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

16. Lograsso, Philip Victor, 1963-. Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis.

Degree: 1992, University of Florida

URL: https://ufdc.ufl.edu/AA00011159

Subjects/Keywords: Acetates; Active sites; Amino acids; Catalysis; Enzymes; Hydrogen; Hydrolysis; pH; Protons; Water tables; Carbonic Anhydrases – genetics ( mesh ); Mutagenesis, Site-Directed ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lograsso, Philip Victor, 1. (1992). Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00011159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lograsso, Philip Victor, 1963-. “Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis.” 1992. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00011159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lograsso, Philip Victor, 1963-. “Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis.” 1992. Web. 19 Jan 2021.

Vancouver:

Lograsso, Philip Victor 1. Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis. [Internet] [Thesis]. University of Florida; 1992. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00011159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lograsso, Philip Victor 1. Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis. [Thesis]. University of Florida; 1992. Available from: https://ufdc.ufl.edu/AA00011159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

17. Douglass, John Wingfield. A ribosomal gene mutation in streptomycin resistant mycobacterium tuberculosis isolates.

Degree: Image, Division of Anatomical Pathology, 1994, University of Cape Town

URL: http://hdl.handle.net/11427/26343

Subjects/Keywords: Mycobacterium Tuberculosis - isolation &; purification; RNA, Ribosomal; Streptomycin - antagonists &; inhibitors; Tuberculosis - Microbiology

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APA (6^th Edition):

Douglass, J. W. (1994). A ribosomal gene mutation in streptomycin resistant mycobacterium tuberculosis isolates. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/26343

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Douglass, John Wingfield. “A ribosomal gene mutation in streptomycin resistant mycobacterium tuberculosis isolates.” 1994. Thesis, University of Cape Town. Accessed January 19, 2021. http://hdl.handle.net/11427/26343.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Douglass, John Wingfield. “A ribosomal gene mutation in streptomycin resistant mycobacterium tuberculosis isolates.” 1994. Web. 19 Jan 2021.

Vancouver:

Douglass JW. A ribosomal gene mutation in streptomycin resistant mycobacterium tuberculosis isolates. [Internet] [Thesis]. University of Cape Town; 1994. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11427/26343.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Douglass JW. A ribosomal gene mutation in streptomycin resistant mycobacterium tuberculosis isolates. [Thesis]. University of Cape Town; 1994. Available from: http://hdl.handle.net/11427/26343

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

18. Allan, Bruce Rider. The evaluation and standardisation of a PCR protocol for the identification of M. tuberculosis in clinical specimens.

Degree: Image, Medicine, 1994, University of Cape Town

URL: http://hdl.handle.net/11427/26179

Subjects/Keywords: Mycobacterium Tuberculosis - isolation &; purification; Polymerase chain reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Allan, B. R. (1994). The evaluation and standardisation of a PCR protocol for the identification of M. tuberculosis in clinical specimens. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/26179

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Allan, Bruce Rider. “The evaluation and standardisation of a PCR protocol for the identification of M. tuberculosis in clinical specimens.” 1994. Thesis, University of Cape Town. Accessed January 19, 2021. http://hdl.handle.net/11427/26179.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Allan, Bruce Rider. “The evaluation and standardisation of a PCR protocol for the identification of M. tuberculosis in clinical specimens.” 1994. Web. 19 Jan 2021.

Vancouver:

Allan BR. The evaluation and standardisation of a PCR protocol for the identification of M. tuberculosis in clinical specimens. [Internet] [Thesis]. University of Cape Town; 1994. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11427/26179.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Allan BR. The evaluation and standardisation of a PCR protocol for the identification of M. tuberculosis in clinical specimens. [Thesis]. University of Cape Town; 1994. Available from: http://hdl.handle.net/11427/26179

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Bertucci, Anthony. Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797).

Degree: Docteur es, Océanographie, 2010, Aix-Marseille 2

URL: http://www.theses.fr/2010AIX22112

►

La formation d’un squelette de CaCO3 par les coraux Scléractiniaires est à la base de l’édification des récifs coralliens. Nombre de ces coraux constructeurs de… (more)

▼

La formation d’un squelette de CaCO3 par les coraux Scléractiniaires est à la base de l’édification des récifs coralliens. Nombre de ces coraux constructeurs de récif vivent en symbiose avec des Dinoflagellés photosynthétiques. Ces deux processus reposent sur le transport et l’utilisation de carbone inorganique (Ci) provenant de l’eau de mer pour la photosynthèse, et du métabolisme animal pour la calcification. Cette thèse s’est intéressée à l’étude moléculaire et physiologique des mécanismes, permettant l’utilisation de ce carbone inorganique.Malgré l’importance des transports de HCO3-, aucun transporteur n’a été caractérisé à cejour et leur implication dans la physiologie des coraux n’est que suggérée par la pharmacologie. Durant cette thèse nous avons cloné un gène codant pour un transporteur deHCO3- chez le corail Acropora sp. La conversion de ce HCO3- en CO2 pour la photosynthèse est facilitée par l’acidification de l’environnement proche du Dinoflagellé dans la cellule animale. Cette acidification est causée par une H+-ATPase de type P que nous avons caractérisée. Ce gène est le premier à montrer une expression dépendante de la vie en symbiose chez le symbiote.Nous avons aussi cloné et localisé deux anhydrases carboniques (AC). L’une impliquée dans la calcification et l’autre dans la régulation du pH intracellulaire et l’équilibre entre leCO2 et HCO3-. Une étude pharmacologique de ces deux AC, a identifié des molécules inhibitrices et activatrices qui ont permis des expériences de physiologie in vivo. Celles-ci permettent une analyse plus discriminante du rôle des AC dans la calcification.

Coral reefs edification is based on the formation of a calcium carbonate skeleton byscleractinian corals. Many of these reef-building corals establish a symbiotic association with photosynthetic Dinoflagellates. Both processes involve the transport and utilization of inorganic carbon (Ci) coming from seawater for photosynthesis, and from animal metabolismfor calcification. This work focused on the molecular and physiological study of poorlyknown mechanisms that allow the utilization of Ci.Despite the importance of bicarbonate transport, no transporter has been characterized and their role in coral physiology is only suggested by pharmacological experiments. We have cloned a gene encoding a bicarbonate transporter in the coral Acropora sp. The conversion of this bicarbonate into CO2 for photosynthesis is mediated by the acidification of the are asurrounding the Dinoflagellate in the animal cell. This is performed by a P type H+-ATPasethat we characterized here. This is the first gene with a symbiosis-dependent expression in the symbiont.This work also allowed the cloning and the localization of two carbonic anhydrases (CA).The first one is involved in calcification, the second one plays a role in the intracellular pHregulation and the CO2 / HCO3- equilibrium. A pharmacological study of these two enzymes identified inhibitor and activator compounds that have been then used in physiology experiments. This last approach…

Advisors/Committee Members: amp;pagesize-30">Allemand, Denis (thesis director), amp;pagesize-30">Zoccola, Didier (thesis director).

Subjects/Keywords: Scléractiniaires; Stylophora pistillata; Carbone inorganique; Biominéralisation; Mécanismes de concentrations du CO2; Anhydrases carboniques; Scleractinian; Stylophora pistillata; Inorganic carbon; Biomineralization; CO2- concentrating-mechanism; Carbonic anhydrase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bertucci, A. (2010). Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797). (Doctoral Dissertation). Aix-Marseille 2. Retrieved from http://www.theses.fr/2010AIX22112

Chicago Manual of Style (16^th Edition):

Bertucci, Anthony. “Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797).” 2010. Doctoral Dissertation, Aix-Marseille 2. Accessed January 19, 2021. http://www.theses.fr/2010AIX22112.

MLA Handbook (7^th Edition):

Bertucci, Anthony. “Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797).” 2010. Web. 19 Jan 2021.

Vancouver:

Bertucci A. Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797). [Internet] [Doctoral dissertation]. Aix-Marseille 2; 2010. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2010AIX22112.

Council of Science Editors:

Bertucci A. Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797). [Doctoral Dissertation]. Aix-Marseille 2; 2010. Available from: http://www.theses.fr/2010AIX22112

20. Ozak Munoz, Omer. Essays on Geographical, Technological, and Cultural Determinants of Comparative Economic Development.

Degree: PhD, Economics, 2011, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:11249/

► This dissertation studies the geographical, technological, and cultural determinants of comparative economic development. The first chapter constructs new measures of distance and transportation costs for… (more)

▼ This dissertation studies the geographical, technological, and cultural determinants of comparative economic development. The first chapter constructs new measures of distance and transportation costs for the pre-industrial era, by finding the cost-minimizing paths between regions, where the cost of travel is measured in time and determined by geographical, technological, and biological conditions. I compare the new measures of the total travel time along the optimal path among various cities with historical data on trade and pilgrimage routes, the speed of news diffusion to Venice during the 16th-18th century, and genetic, linguistic, and religious distances among countries. Performing various tests I show that the new measures outperform commonly used great circle distances. These results show that these measures, and the indices on which they are based, capture well the levels of interaction and the technology of movement in the pre-industrial era, allowing for a wide range of possible applications. Chapter 2 exploits cross-country variation in the degree of geographical isolation, prior to the advent of sea-faring and airborne transportation technologies, to examine its impact on the course of economic development across the globe. The empirical investigation establishes that prehistoric geographical isolation has generated a persistent beneficial effect on the process of development and contributed to the contemporary variation in the standard of living across countries. In chapter 3 I propose that the distance from the pre-industrial technological frontier has a non-monotonic and persistent effect on development. While remoteness from this frontier diminished imitation, it fostered the emergence of a culture conducive to innovation and knowledge creation, which has persisted into the modern era, once barriers to movement dissipated. Using the new measures I show that the distance to the technological frontier in the past has a robust and persistent U-shaped relation with measures of economic development both in the pre-industrial and modern eras. Advisors/Committee Members: amp;pagesize-30">Galor, Oded (Director), amp;pagesize-30">Howitt, Peter (Reader), amp;pagesize-30">Levine, Ross (Reader), amp;pagesize-30">Weil, David (Reader).

Subjects/Keywords: isolation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ozak Munoz, O. (2011). Essays on Geographical, Technological, and Cultural Determinants of Comparative Economic Development. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:11249/

Chicago Manual of Style (16^th Edition):

Ozak Munoz, Omer. “Essays on Geographical, Technological, and Cultural Determinants of Comparative Economic Development.” 2011. Doctoral Dissertation, Brown University. Accessed January 19, 2021. https://repository.library.brown.edu/studio/item/bdr:11249/.

MLA Handbook (7^th Edition):

Ozak Munoz, Omer. “Essays on Geographical, Technological, and Cultural Determinants of Comparative Economic Development.” 2011. Web. 19 Jan 2021.

Vancouver:

Ozak Munoz O. Essays on Geographical, Technological, and Cultural Determinants of Comparative Economic Development. [Internet] [Doctoral dissertation]. Brown University; 2011. [cited 2021 Jan 19]. Available from: https://repository.library.brown.edu/studio/item/bdr:11249/.

Council of Science Editors:

Ozak Munoz O. Essays on Geographical, Technological, and Cultural Determinants of Comparative Economic Development. [Doctoral Dissertation]. Brown University; 2011. Available from: https://repository.library.brown.edu/studio/item/bdr:11249/

University of Hawaii – Manoa

21. Tomita, Dean K. The use and perception of social network sites in telecommuting work environments.

Degree: 2015, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100361

►

Ph.D. University of Hawaii at Manoa 2014.

For the past forty years, communications technologies available to the telecommuter have remained basically the same, mainly offering… (more)

Subjects/Keywords: isolation

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APA (6^th Edition):

Tomita, D. K. (2015). The use and perception of social network sites in telecommuting work environments. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100361

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tomita, Dean K. “The use and perception of social network sites in telecommuting work environments.” 2015. Thesis, University of Hawaii – Manoa. Accessed January 19, 2021. http://hdl.handle.net/10125/100361.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tomita, Dean K. “The use and perception of social network sites in telecommuting work environments.” 2015. Web. 19 Jan 2021.

Vancouver:

Tomita DK. The use and perception of social network sites in telecommuting work environments. [Internet] [Thesis]. University of Hawaii – Manoa; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10125/100361.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomita DK. The use and perception of social network sites in telecommuting work environments. [Thesis]. University of Hawaii – Manoa; 2015. Available from: http://hdl.handle.net/10125/100361

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

22. Baxter, Ross McPherson, 1918-. A study of some poisonous mushrooms.

Degree: 1951, University of Florida

URL: https://ufdc.ufl.edu/AA00059073

Subjects/Keywords: Alkaloids; Bottles; Crystals; Ergot alkaloids; Mushrooms; Mycelium; Oxytocics; pH; Solvents; Uterus; Basidiomycota ( mesh ); Mannitol – isolation &; purification ( mesh ); Oxytocics – isolation &; purification ( mesh ); Pharmacy thesis Ph. D

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baxter, Ross McPherson, 1. (1951). A study of some poisonous mushrooms. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00059073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baxter, Ross McPherson, 1918-. “A study of some poisonous mushrooms.” 1951. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00059073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baxter, Ross McPherson, 1918-. “A study of some poisonous mushrooms.” 1951. Web. 19 Jan 2021.

Vancouver:

Baxter, Ross McPherson 1. A study of some poisonous mushrooms. [Internet] [Thesis]. University of Florida; 1951. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00059073.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baxter, Ross McPherson 1. A study of some poisonous mushrooms. [Thesis]. University of Florida; 1951. Available from: https://ufdc.ufl.edu/AA00059073

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Thébault, Simon Romain. Contribution à l'évaluation in situ des performances d'isolation thermique de l'enveloppe des bâtiments : In situ assessment of the thermal insulation performance of building envelopes.

Degree: Docteur es, Thermique - Énergétique, 2017, Lyon

URL: http://www.theses.fr/2017LYSEI008

►

Dans un contexte d’économie d’énergie et de réduction des émissions de gaz à effet de serre, de nombreux efforts ont été réalisés en France pour… (more)

▼

Dans un contexte d’économie d’énergie et de réduction des émissions de gaz à effet de serre, de nombreux efforts ont été réalisés en France pour renforcer l’isolation de l’enveloppe des bâtiments afin de contribuer à réduire les consommations de chauffage. Toutefois, il arrive souvent que la performance thermique calculée avant construction ou rénovation ne soit pas atteinte sur le terrain (erreur de calcul, défauts de mise en œuvre, etc.). Or, pour pouvoir généraliser la construction de bâtiments à basse consommation et la rénovation, il faut pouvoir garantir aux maîtres d'ouvrage une performance réelle de leur bâtiment après travaux. Le fait de mesurer in situ la performance intrinsèque d'isolation thermique de l'enveloppe permet de contribuer à cette garantie. Il existe à l’échelle internationale de nombreuses méthodes basées sur le suivi des consommations et des conditions thermiques intérieures et extérieures. Certaines ont déjà fait leurs preuves sur le terrain, mais sont souvent soit contraignantes, soit peu précises. Et surtout, les calculs d’incertitude associés sont souvent rudimentaires. L’objectif de ce travail financé par le CSTB est de consolider scientifiquement une nouvelle méthode de mesure de la qualité d’isolation globale d’un bâtiment à réception des travaux (méthode ISABELE). Dans le premier chapitre, un état de l'art sur les méthodes existantes a été réalisé afin de dégager des pistes d'amélioration sur la base d'une synthèse comparative. La piste prioritaire identifiée porte sur le calcul d'incertitude (un point central du problème). La propagation des erreurs aléatoires par un approche bayésienne ainsi que des erreurs systématiques par une approche plus classique feront l'objet de la méthodologie globale proposée dans le second chapitre. L'une des importantes sources d'incertitude porte sur l'évaluation du débit d'infiltration. La caractérisation de cette incertitude et de l'impact sur le résultat de mesure fera l'objet du troisième chapitre, avec un comparatif de différentes approches expérimentales (règle du pouce, modèles aérauliques, gaz traceur). Enfin, une amélioration de la prise en compte de la dynamique thermique du bâtiment au cours du test sera proposée dans le dernier chapitre. Son fondement repose sur l'adaptation du modèle thermique inverse en fonction du bâtiment et des conditions du test. Pour cela, une sélection parmi une banque de modèles simplifiés est réalisée sur la base de critères statistiques et du principe de parcimonie. Ces différentes dispositions ont été testés sur une large série de mesures menées sur un même bâtiment à ossature bois (chalet OPTIMOB). La robustesse et la précision du résultat de mesure ont ainsi pu être légèrement améliorées. La méthode de calcul du débit d'infiltration, ni trop simple ni trop complexe, a pu également être validée. Enfin, le temps de mesure minimal nécessaire a pu être déterminé en fonction de la classe d'inertie du bâtiment.

The global context of energy savings and greenhouse gases emissions control led to significant efforts…

Advisors/Committee Members: amp;pagesize-30">Kuznik, Frédéric (thesis director), amp;pagesize-30">Roux, Jean-Jacques (thesis director).

Subjects/Keywords: Energie; Energétique; Gaz carbonique; Performance thermique; Bâtiment basse consommation; Calcul d incertitude; Isolation thermique; Débit; Dynamique thermique; Classe inertie; Energy; Energetic; Carbonic gases; Thermal performance; Low-Energy building; Unvertainly calculation; Thermal insulation; Flow; Thermal dynamic; Inertia class; 536.072

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thébault, S. R. (2017). Contribution à l'évaluation in situ des performances d'isolation thermique de l'enveloppe des bâtiments : In situ assessment of the thermal insulation performance of building envelopes. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2017LYSEI008

Chicago Manual of Style (16^th Edition):

Thébault, Simon Romain. “Contribution à l'évaluation in situ des performances d'isolation thermique de l'enveloppe des bâtiments : In situ assessment of the thermal insulation performance of building envelopes.” 2017. Doctoral Dissertation, Lyon. Accessed January 19, 2021. http://www.theses.fr/2017LYSEI008.

MLA Handbook (7^th Edition):

Thébault, Simon Romain. “Contribution à l'évaluation in situ des performances d'isolation thermique de l'enveloppe des bâtiments : In situ assessment of the thermal insulation performance of building envelopes.” 2017. Web. 19 Jan 2021.

Vancouver:

Thébault SR. Contribution à l'évaluation in situ des performances d'isolation thermique de l'enveloppe des bâtiments : In situ assessment of the thermal insulation performance of building envelopes. [Internet] [Doctoral dissertation]. Lyon; 2017. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2017LYSEI008.

Council of Science Editors:

Thébault SR. Contribution à l'évaluation in situ des performances d'isolation thermique de l'enveloppe des bâtiments : In situ assessment of the thermal insulation performance of building envelopes. [Doctoral Dissertation]. Lyon; 2017. Available from: http://www.theses.fr/2017LYSEI008

University of Florida

24. Seifert, Kyle Nikoli, 1975-. Characterization of group B streptococcal surface antigens delta, epsilon, and glyceraldehyde-3-phosphate dehydrogenase.

Degree: 2004, University of Florida

URL: https://ufdc.ufl.edu/AA00011189

Subjects/Keywords: Antibodies; Antigens; Antiserum; Diseases; DNA; Open reading frames; Polymerase chain reaction; Polysaccharides; Proteins; Reactivity; Antigens – genetics ( mesh ); Antigens – isolation &; purification ( mesh ); Glyceraldehyde-3-Phosphate Dehydrogenases – genetics ( mesh ); Glyceraldehyde-3-Phosphate Dehydrogenases – isolation &; purification ( mesh ); Streptococcus – genetics ( mesh ); Streptococcus – isolation &; purification ( mesh ); Streptococcus – pathogenicity ( mesh )

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APA (6^th Edition):

Seifert, Kyle Nikoli, 1. (2004). Characterization of group B streptococcal surface antigens delta, epsilon, and glyceraldehyde-3-phosphate dehydrogenase. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00011189

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Seifert, Kyle Nikoli, 1975-. “Characterization of group B streptococcal surface antigens delta, epsilon, and glyceraldehyde-3-phosphate dehydrogenase.” 2004. Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/AA00011189.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Seifert, Kyle Nikoli, 1975-. “Characterization of group B streptococcal surface antigens delta, epsilon, and glyceraldehyde-3-phosphate dehydrogenase.” 2004. Web. 19 Jan 2021.

Vancouver:

Seifert, Kyle Nikoli 1. Characterization of group B streptococcal surface antigens delta, epsilon, and glyceraldehyde-3-phosphate dehydrogenase. [Internet] [Thesis]. University of Florida; 2004. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/AA00011189.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Seifert, Kyle Nikoli 1. Characterization of group B streptococcal surface antigens delta, epsilon, and glyceraldehyde-3-phosphate dehydrogenase. [Thesis]. University of Florida; 2004. Available from: https://ufdc.ufl.edu/AA00011189

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

25. Galib,Mirza. Insights into the reactivity of H2CO3 and its derivatives in bulk water, water clusters, and at the air/water interface: Ab initio molecular dynamics and metadynamics studies.

Degree: PhD, Department of Chemistry, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/c8p58pd05n

► H2CO3 can decompose into CO2 in water either via the water route (H2CO3 ↔ CO2+H2O ) or the hydroxide route (H2CO3 + H2O ↔ CO2+OH−+H3O+).… (more)

▼ H2CO3 can decompose into CO2 in water either via the water route (H2CO3 ↔ CO2+H2O ) or the hydroxide route (H2CO3 + H2O ↔ CO2+OH−+H3O+). The water route reactions play a fundamental role in the global carbon cycle, and the hydroxide route reactions play an important role in the regulation of blood pH and the transport of CO2 during respiration. Despite the plethora of experimental work on these reactions in water, a microscopic understanding of the underlying mechanisms was lacking due to a shortage of theoretical work. In this thesis, we employed Car-Parrinello molecular dynamics and metadynamics to ascertain the microscopic mechanisms and compute the free energy changes and barriers of a series of reactions involving H2CO3, HCO3- , and CO2 in bulk water, water clusters, and at the air/water interface in order to shed much needed light on these reactions. Before delving into the reactivity of H2CO3 in water, we investigated the energetics and mechanisms of the conformational changes of the cis-cis (CC), cis-trans (CT), and trans-trans (TT) conformers of H2CO3 in water. The free energy barriers/changes for the various conformational changes via the change in one of the two dihedral angles were calculated and contrasted with the previously calculated values for the gas phase. We then investigated the energetics and mechanisms of the dissociation (H2CO3 ↔ HCO3− + H+) and hydroxide-route decomposition (HCO3- → CO2 + OH−) of all three conformers of H2CO3 in water. The CT and TT conformers were found to undergo decomposition in water via a two-step process: dissociation followed by decomposition. This is in contrast with the concerted mechanism proposed for the gas phase, which involves a dehydroxylation of one of the OH groups and a simultaneous deprotonation of the other OH group to yield CO2 and H2O. Our calculated pKa values and decomposition free energy barriers for the CT and TT conformers are consistent with the experimental values. The decomposition of H2CO3 in diﬀerent-sized water clusters was investigated to determine whether the concerted or step-wise mechanism predominates in bulk water. We found that in the small clusters (containing 6 and 9 water molecules), the decomposition occurs via a concerted proton shuttle mechanism involving a cyclic transition state, whereas in the larger clusters (containing 20 and 45 water molecules), the decomposition follows a two-step mechanism involving a solvent-separated HCO3- /H3O+ ion pair intermediate. The larger clusters contain a suﬃcient number of water molecules to fully solvate the H3O+ intermediate, a prerequisite for the step-wise reaction. Our results demonstrated that the decomposition of H2CO3 predominantly occurs via the step-wise mechanism in bulk water. The dissociation of H2CO3 at the air/water interface was then studied. Our results indicated that H2CO3 dissociates faster at the water surface than in bulk water, in contrast to recent experiments and simulations which have shown that HNO3 has a lower propensity to dissociate at the water surface than in bulk…

Subjects/Keywords: metadynamics; carbonic acid; cpmd

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APA (6^th Edition):

Galib,Mirza. (2014). Insights into the reactivity of H2CO3 and its derivatives in bulk water, water clusters, and at the air/water interface: Ab initio molecular dynamics and metadynamics studies. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/c8p58pd05n

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Galib,Mirza. “Insights into the reactivity of H2CO3 and its derivatives in bulk water, water clusters, and at the air/water interface: Ab initio molecular dynamics and metadynamics studies.” 2014. Doctoral Dissertation, University of Alberta. Accessed January 19, 2021. https://era.library.ualberta.ca/files/c8p58pd05n.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Galib,Mirza. “Insights into the reactivity of H2CO3 and its derivatives in bulk water, water clusters, and at the air/water interface: Ab initio molecular dynamics and metadynamics studies.” 2014. Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Galib,Mirza. Insights into the reactivity of H2CO3 and its derivatives in bulk water, water clusters, and at the air/water interface: Ab initio molecular dynamics and metadynamics studies. [Internet] [Doctoral dissertation]. University of Alberta; 2014. [cited 2021 Jan 19]. Available from: https://era.library.ualberta.ca/files/c8p58pd05n.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Galib,Mirza. Insights into the reactivity of H2CO3 and its derivatives in bulk water, water clusters, and at the air/water interface: Ab initio molecular dynamics and metadynamics studies. [Doctoral Dissertation]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/c8p58pd05n

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

University of Hawaii – Manoa

26. Uchikawa, Joji. Effect of seawater pH, Mg2+ and carbonic anhydrase on marine biogenic carbonates and their δ18O values : future and paleo applications.

Degree: 2016, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/101037

►

Ph.D. University of Hawaii at Manoa 2012.

Ocean pH affects marine CaCO3 cycling and the oxygen isotope (δ18O) values of biogenic CaCO3. Based on a… (more)

▼

Ph.D. University of Hawaii at Manoa 2012.

Ocean pH affects marine CaCO3 cycling and the oxygen isotope (δ18O) values of biogenic CaCO3. Based on a carbon – cycle model and laboratory experiments, this study explores future perspectives of ocean acidification and characterizes the mechanism and paleoclimatic implications of the pH effect on δ18O values of biogenic CaCO3. For a release of 5,000 Pg of carbon in 500 years, the surface ocean pH and atmospheric CO2 will rise to 7.4 and 1,900 ppmv. Eventually anthropogenic CO2 will be sequestered via continental weathering followed by CaCO3 burial in sediments. But these processes will not effectively mitigate the predicted carbon – cycle perturbations on centennial timescales. Studying the impact of CO2 – forcing on global temperatures from past climate events is crucial for predicting future global warming. But paleotemperature reconstructions using δ18O values of biogenic CaCO3 can be hampered by past changes in seawater pH. For example, planktonic foraminiferal δ18O values could have been biased by +0.4‰ at maximum (up to 2 °C of underestimated temperature) due to pH decline during the Paleocene – Eocene Thermal Maximum (~56 Ma). A proposed explanation for the pH effect on δ18O values of biogenic CaCO3 [Zeebe, 1999, 2007] assumes 18O equilibrium in the CO2 – H2O system in the calcification microenvironments and that equilibrium fractionation between dissolved CO2 species and H2O by Beck et al. [2005] holds in seawater. These assumptions were evaluated by quantitative BaCO3 precipitation experiments focusing on the effect of carbonic anhydrase (CA) and Mg2+ on the kinetics and equilibrium of 18O partitioning in the CO2 – H2O system. CA accelerates 18O equilibration in the CO2 – H2O system by catalyzing CO2 hydration. Calculations suggest that 18O equilibration within the timescales of calcification is possible with 10 – 8 to 10 – 7 M of CA. Mg2+ is the most important cation for complex formation with CO32 – in seawater. Although the MgCO30 abundance in the total dissolved CO2 was varied up to 30% in the experiments, the results revealed no discernible influence on equilibrium 18O fractionation in the CO2 – H2O system. These outcomes contribute to fundamental understanding of vital effects on δ18O values of biogenic CaCO3.

Subjects/Keywords: ocean pH; Mg2+; carbonic anhydrase

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APA (6^th Edition):

Uchikawa, J. (2016). Effect of seawater pH, Mg2+ and carbonic anhydrase on marine biogenic carbonates and their δ18O values : future and paleo applications. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/101037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Uchikawa, Joji. “Effect of seawater pH, Mg2+ and carbonic anhydrase on marine biogenic carbonates and their δ18O values : future and paleo applications.” 2016. Thesis, University of Hawaii – Manoa. Accessed January 19, 2021. http://hdl.handle.net/10125/101037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Uchikawa, Joji. “Effect of seawater pH, Mg2+ and carbonic anhydrase on marine biogenic carbonates and their δ18O values : future and paleo applications.” 2016. Web. 19 Jan 2021.

Vancouver:

Uchikawa J. Effect of seawater pH, Mg2+ and carbonic anhydrase on marine biogenic carbonates and their δ18O values : future and paleo applications. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10125/101037.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Uchikawa J. Effect of seawater pH, Mg2+ and carbonic anhydrase on marine biogenic carbonates and their δ18O values : future and paleo applications. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/101037

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

27. DiMario, Robert John. The Characterization of Alpha- and Beta-Carbonic Anhydrases of Arabidopsis thaliana.

Degree: PhD, 2016, Louisiana State University

URL: etd-03302016-075902 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3758

► Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO2 and HCO3-. In Arabidopsis thaliana, there are eight Alpha-CA genes, six Beta-CA… (more)

Subjects/Keywords: carbonic anhydrase; photosynthesis; plant biology

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APA (6^th Edition):

DiMario, R. J. (2016). The Characterization of Alpha- and Beta-Carbonic Anhydrases of Arabidopsis thaliana. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-03302016-075902 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3758

Chicago Manual of Style (16^th Edition):

DiMario, Robert John. “The Characterization of Alpha- and Beta-Carbonic Anhydrases of Arabidopsis thaliana.” 2016. Doctoral Dissertation, Louisiana State University. Accessed January 19, 2021. etd-03302016-075902 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3758.

MLA Handbook (7^th Edition):

DiMario, Robert John. “The Characterization of Alpha- and Beta-Carbonic Anhydrases of Arabidopsis thaliana.” 2016. Web. 19 Jan 2021.

Vancouver:

DiMario RJ. The Characterization of Alpha- and Beta-Carbonic Anhydrases of Arabidopsis thaliana. [Internet] [Doctoral dissertation]. Louisiana State University; 2016. [cited 2021 Jan 19]. Available from: etd-03302016-075902 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3758.

Council of Science Editors:

DiMario RJ. The Characterization of Alpha- and Beta-Carbonic Anhydrases of Arabidopsis thaliana. [Doctoral Dissertation]. Louisiana State University; 2016. Available from: etd-03302016-075902 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3758

Tampere University

28. Bootorabi, (Ahmad) Fatemeh. Production and Characterization of Carbonic Anhydrase VII. Acetaldehyde-derived modifications and comparison to the other cytosolic isozymes .

Degree: 2011, Tampere University

URL: https://trepo.tuni.fi/handle/10024/66751

► Väitöskirjatutkimuksessa tuotettiin bakteerisoluissa ihmisen hiilihappoanhydraasi VII isoentsyymi (CA VII), jota karakterisoitiin tutkimuksen aikana. Paikantamistutkimuksissa todettiin, että CA VII isoentsyymiä esiintyy runsaasti erityisesti maksassa ja aivokasvaimissa.… (more)

▼ Väitöskirjatutkimuksessa tuotettiin bakteerisoluissa ihmisen hiilihappoanhydraasi VII isoentsyymi (CA VII), jota karakterisoitiin tutkimuksen aikana. Paikantamistutkimuksissa todettiin, että CA VII isoentsyymiä esiintyy runsaasti erityisesti maksassa ja aivokasvaimissa. Tulosten perusteella CA VII on lupaava merkkiproteiini aivokasvainten diagnostiikassa. Tutkimuksen erityisenä tavoitteena oli selvittää muutoksia, joita alkoholin aineenvaihduntatuote, asetaldehydi, aiheuttaa hiilihappoanhydraasien proteiinirakenteessa. Kohteeksi valittiin kaikki solulimassa ilmentyvät hiilihappoanhydraasit, CA I, CA II, CA III, CA VII ja CA XIII. Tulosten perusteella asetaldehydi sitoutui voimakkaimmin CA II isoentsyymiin. Vaikka yhteen entsyymimolekyyliin sitoutui jopa 19 asetaldehydimolekyyliä, tämä rakennemuutos laski odotettua vähemmän hiilihappoanhydraasin entsymaattista aktiivisuutta. Väitöskirjatutkimuksesta saadut tiedot auttavat ymmärtämään paremmin hiilihappoanhydraasien toimintaa sekä asetaldehydin sitoutumista ja vaikutuksia entsyymeissä.; Carbonic anhydrase (CA) enzymes are ubiquitous metalloenzymes which are able to maintain acid-base homeostasis by catalyzing the reversible conversion of carbon dioxide to bicarbonate ions and protons. This family of enzymes plays an important role for the survival of the cell and whole organism by contributing to various physiological functions. The ?-CA family consists of fifteen isozymes of which thirteen are expressed and have catalytically active forms in mammals. The CA isozymes have shown unique expression patterns, kinetic and inhibitory properties, as well as several different subcellular localizations: five isozymes are cytosolic, five are membrane-associated, two are mitochondrial, and one is a secretory form. One major aim of our study was to produce and characterize human CA VII, a cytosolic isozyme. Two potential variants of CA VII were discovered based on GeneBank data. The investigations on the CA VII expression in the human cerebrum and hippocampus showed positive mRNA signals for both variants. The shorter variant turned out to be unstable and was not detectable in any murine tissues at the protein level. The full-length CA VII was expressed in the liver, colon, and muscle. In addition to certain normal tissues, CA VII was detected in gliomas. Our results indicated that CA VII expression became higher in high-grade tumors, suggesting an important role for this enzyme in tumor metabolism. This result led us to conclude that CA VII might serve as a novel potential biomarker of poor prognosis in diffuse astrocytomas. The other main goal was to investigate and compare acetaldehyde-derived modifications in five cytosolic CAs, including CA I, II, III, VII, and XIII. Acetaldehyde, the first metabolite of ethanol, has been shown to be a toxic compound in the body, because of its capablity to form stable and unstable adducts with various proteins and cellular components. The acetaldehyde binding can result in functional and structural changes in proteins, which can further…

Subjects/Keywords: Carbonic Anhydrase VII ; Acetaldehyde ; Characterization

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APA (6^th Edition):

Bootorabi, (. F. (2011). Production and Characterization of Carbonic Anhydrase VII. Acetaldehyde-derived modifications and comparison to the other cytosolic isozymes . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/66751

Chicago Manual of Style (16^th Edition):

Bootorabi, (Ahmad) Fatemeh. “Production and Characterization of Carbonic Anhydrase VII. Acetaldehyde-derived modifications and comparison to the other cytosolic isozymes .” 2011. Doctoral Dissertation, Tampere University. Accessed January 19, 2021. https://trepo.tuni.fi/handle/10024/66751.

MLA Handbook (7^th Edition):

Bootorabi, (Ahmad) Fatemeh. “Production and Characterization of Carbonic Anhydrase VII. Acetaldehyde-derived modifications and comparison to the other cytosolic isozymes .” 2011. Web. 19 Jan 2021.

Vancouver:

Bootorabi (F. Production and Characterization of Carbonic Anhydrase VII. Acetaldehyde-derived modifications and comparison to the other cytosolic isozymes . [Internet] [Doctoral dissertation]. Tampere University; 2011. [cited 2021 Jan 19]. Available from: https://trepo.tuni.fi/handle/10024/66751.

Council of Science Editors:

Bootorabi (F. Production and Characterization of Carbonic Anhydrase VII. Acetaldehyde-derived modifications and comparison to the other cytosolic isozymes . [Doctoral Dissertation]. Tampere University; 2011. Available from: https://trepo.tuni.fi/handle/10024/66751

King Abdullah University of Science and Technology

29. Vancea, Alexandra. Activity Assessment of a Halophilic γ-carbonic Anhydrase from the Red Sea Brine Pool Discovery Deep.

Degree: 2019, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/652899

► Carbonic anhydrases catalyze a central reaction in life – the inter-conversion between carbon dioxide and water. Consequently, there is an increasing interest in research in… (more)

Subjects/Keywords: Carbonic Anhydrase; Halophilic; Gamma Class

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APA (6^th Edition):

Vancea, A. (2019). Activity Assessment of a Halophilic γ-carbonic Anhydrase from the Red Sea Brine Pool Discovery Deep. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/652899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vancea, Alexandra. “Activity Assessment of a Halophilic γ-carbonic Anhydrase from the Red Sea Brine Pool Discovery Deep.” 2019. Thesis, King Abdullah University of Science and Technology. Accessed January 19, 2021. http://hdl.handle.net/10754/652899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancea, Alexandra. “Activity Assessment of a Halophilic γ-carbonic Anhydrase from the Red Sea Brine Pool Discovery Deep.” 2019. Web. 19 Jan 2021.

Vancouver:

Vancea A. Activity Assessment of a Halophilic γ-carbonic Anhydrase from the Red Sea Brine Pool Discovery Deep. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2019. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10754/652899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vancea A. Activity Assessment of a Halophilic γ-carbonic Anhydrase from the Red Sea Brine Pool Discovery Deep. [Thesis]. King Abdullah University of Science and Technology; 2019. Available from: http://hdl.handle.net/10754/652899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Phillips, Tamsin Kate. Understanding women's engagement in HIV care after initiating antiretroviral therapy during pregnancy in South Africa.

Degree: PhD, Public Health and Family Medicine, 2019, University of Cape Town

URL: http://hdl.handle.net/11427/30428

► Background: Sustained engagement in HIV care, including adherence to antiretroviral therapy (ART) and retention in HIV services, is essential to optimize maternal health and prevent… (more)

▼ Background: Sustained engagement in HIV care, including adherence to antiretroviral therapy (ART) and retention in HIV services, is essential to optimize maternal health and prevent perinatal, postnatal and sexual HIV transmission. However, engagement in care remains a substantial challenge for pregnant and postpartum women. Women’s experience of and response to barriers to engagement in care, including ART side effects, transfer of care and mobility, may be altered by the transitions experienced in pregnancy and motherhood, and there have been few quantitative analyses of these risk factors in maternal ART cohorts. The way engagement in HIV care is measured also varies widely and no gold standard measures of ART adherence or retention exist. Composite assessments of adherence and retention, including drug concentrations, longitudinal self-reported adherence, and interlinked routine electronic health data, have not been thoroughly evaluated among African women living with HIV. To address these gaps in knowledge, this thesis investigates novel measures of ART adherence, and evaluates interlinked routine electronic health data to measure retention in a South African maternal ART cohort. It describes maternal engagement in HIV care, and examines barriers to engagement that require consideration specific to maternal ART. Methods: This research included women who initiated ART during pregnancy in a large integrated antenatal care and ART clinic in Gugulethu, South Africa (2013-2014). Until July 2013, only women who met certain clinical criteria (CD4 cell count <350 cells/µL or disease stage III or IV) were eligible for lifelong ART, thereafter guidelines changed to recommend lifelong ART for all pregnant women living with HIV. In this setting, all women receive ART and antenatal care in an integrated clinic during pregnancy and are required to transfer to a general ART clinic postpartum. Data were obtained from questionnaires (including demographics, self-reported ART adherence and self-reported side effects) and blood specimens (for HIV viral load) collected at study visits approximately every three months from pregnancy through 18 months postpartum. One additional visit took place 3-4 years postpartum where blood specimens for drug concentrations were also collected. In parallel, routine electronic data, linked across clinics and data sources including HIV clinical visits, laboratory testing and pharmacy dispensing data, were obtained through 30 months on ART. Findings: Substantial disengagement from care, both non-adherence and non-retention, was observed in all analyses. At least one ART side effect was reported by 97% of women during pregnancy and high overall side effect burden was associated with reported missed ART doses. Retention worsened over the first two years on ART and 21% of women were lost immediately after transfer from the integrated clinic. Women who linked to care spread to multiple different ART clinics after transfer; 21% moved clinics two or more times. Using combined routine medical records, only… Advisors/Committee Members: amp;pagesize-30">Myer, Landon (advisor), amp;pagesize-30">Orrell, Catherine (advisor).

Subjects/Keywords: Epidemiology &; Biostatistics

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APA (6^th Edition):

Phillips, T. K. (2019). Understanding women's engagement in HIV care after initiating antiretroviral therapy during pregnancy in South Africa. (Doctoral Dissertation). University of Cape Town. Retrieved from http://hdl.handle.net/11427/30428

Chicago Manual of Style (16^th Edition):

Phillips, Tamsin Kate. “Understanding women's engagement in HIV care after initiating antiretroviral therapy during pregnancy in South Africa.” 2019. Doctoral Dissertation, University of Cape Town. Accessed January 19, 2021. http://hdl.handle.net/11427/30428.

MLA Handbook (7^th Edition):

Phillips, Tamsin Kate. “Understanding women's engagement in HIV care after initiating antiretroviral therapy during pregnancy in South Africa.” 2019. Web. 19 Jan 2021.

Vancouver:

Phillips TK. Understanding women's engagement in HIV care after initiating antiretroviral therapy during pregnancy in South Africa. [Internet] [Doctoral dissertation]. University of Cape Town; 2019. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11427/30428.

Council of Science Editors:

Phillips TK. Understanding women's engagement in HIV care after initiating antiretroviral therapy during pregnancy in South Africa. [Doctoral Dissertation]. University of Cape Town; 2019. Available from: http://hdl.handle.net/11427/30428

Open Access Theses and Dissertations