subject:(Carbonic Anhydrases genetics mesh )

University of Hawaii – Manoa

1. Pal, Archana. Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala.

Degree: 2016, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100737

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Ph.D. University of Hawaii at Manoa 2013.

Carbonic anhydrases (CAs) catalyze rapid interconversion of CO2 and water to bicarbonate and protons. Carbonic anhydrases (CAs) have… (more)

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Ph.D. University of Hawaii at Manoa 2013.

Carbonic anhydrases (CAs) catalyze rapid interconversion of CO2 and water to bicarbonate and protons. Carbonic anhydrases (CAs) have been extensively studied and are known to be involved in various physiological processes such as respiration, cell growth and acid/base regulation in animals, and carbon fixation and photosynthesis in plants, algae and cyanobacteria. Although the beta-type carbonic anhydrases (β-CAs) are crucial for carbon fixation in C4 plants, algae, and cyanobacteria, the role of β-CA in C3 plants is not well-studied. Therefore, the specific objective of this research is to isolate and characterize the genes encoding chloroplastic and cytoplasmic β-CAs from the C3 plant, Leucaena leucocephala (leucaena) and investigate their possible role in the plant by overexpressing the leucaena β-CA in Nicotiana tabacum and also to study the expression pattern of leucaena β-CAs under various physiological stress conditions that affect photosynthesis potential in plants. In this study, two cDNA clones, cacp and cacyt, encoding chloroplastic and cytoplasmic forms of β-CA, respectively, were isolated from a leucaena cDNA library. These clones were further extended to full-length by 5'-and 3'-rapid amplification of cDNA ends (RACE). Deduced amino acid sequences of cacp and cacyt exhibited 79 % homology with each other. To determine the possible role of these genes in leucaena under physiological stresses, quantitative PCR (qPCR) was performed from different tissues of leucaena grown under normal, drought, saline, light, bright light, and dark conditions. The expression of cacp and cacyt was found to be up regulated under drought, salt, and light conditions and down regulated under dark conditions. Bright light conditions, compared with normal light condition, did not exhibit much change in the transcript levels of cacp and cacyt. Additionally, tobacco plants were transformed with leucaena's cacp and cacyt separately and also together resulting in three types of transgenic tobacco plants (i) with cacp only (ii) with cacyt only and (iii) with both cacp and cacyt. These transgenic tobaccos were also studied under stress conditions. This study will give us a deeper insight to understand the role of chloroplastic and cytoplasmic β-CAs in leucaena and also in C3 plants in general.

Subjects/Keywords: Carbonic anhydrases; CAs

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APA (6^th Edition):

Pal, A. (2016). Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100737

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pal, Archana. “Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala.” 2016. Thesis, University of Hawaii – Manoa. Accessed January 20, 2021. http://hdl.handle.net/10125/100737.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pal, Archana. “Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala.” 2016. Web. 20 Jan 2021.

Vancouver:

Pal A. Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10125/100737.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pal A. Functional characterization of chloroplastic and cytoplasmic β-carbonic anhydrase isoforms from leucaena leucocephala. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/100737

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

2. Lograsso, Philip Victor, 1963-. Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis.

Degree: 1992, University of Florida

URL: https://ufdc.ufl.edu/AA00011159

Subjects/Keywords: Acetates; Active sites; Amino acids; Catalysis; Enzymes; Hydrogen; Hydrolysis; pH; Protons; Water tables; Carbonic Anhydrases – genetics ( mesh ); Mutagenesis, Site-Directed ( mesh )

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APA (6^th Edition):

Lograsso, Philip Victor, 1. (1992). Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00011159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lograsso, Philip Victor, 1963-. “Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis.” 1992. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00011159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lograsso, Philip Victor, 1963-. “Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis.” 1992. Web. 20 Jan 2021.

Vancouver:

Lograsso, Philip Victor 1. Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis. [Internet] [Thesis]. University of Florida; 1992. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00011159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lograsso, Philip Victor 1. Source of the catalytic differences between human carbonic anhydrases III and II as determined by site-specific mutagenesis. [Thesis]. University of Florida; 1992. Available from: https://ufdc.ufl.edu/AA00011159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

3. Hurt, Jonathan Dale, 1967-. Isolation and characterization of murine carbonic anydrase IV.

Degree: 1996, University of Florida

URL: https://ufdc.ufl.edu/AA00052899

Subjects/Keywords: Amino acids; Complementary DNA; DNA; Enzymes; Exons; Genomics; Imidazoles; Mice; Proteins; Protons; Amino Acid Sequence ( mesh ); Base Sequence ( mesh ); Carbonic Anhydrases – analysis ( mesh ); Carbonic Anhydrases – genetics ( mesh ); Carbonic Anhydrases – isolation &; purification ( mesh ); Carbonic Anhydrases – pharmacokinetics ( mesh ); Chromosome Mapping ( mesh ); Department of Biochemistry and Molecular Biology thesis Ph.D ( mesh ); Molecular Sequence Data ( mesh ); Protein Folding ( mesh ); Research ( mesh )

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APA (6^th Edition):

Hurt, Jonathan Dale, 1. (1996). Isolation and characterization of murine carbonic anydrase IV. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00052899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hurt, Jonathan Dale, 1967-. “Isolation and characterization of murine carbonic anydrase IV.” 1996. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00052899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hurt, Jonathan Dale, 1967-. “Isolation and characterization of murine carbonic anydrase IV.” 1996. Web. 20 Jan 2021.

Vancouver:

Hurt, Jonathan Dale 1. Isolation and characterization of murine carbonic anydrase IV. [Internet] [Thesis]. University of Florida; 1996. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00052899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hurt, Jonathan Dale 1. Isolation and characterization of murine carbonic anydrase IV. [Thesis]. University of Florida; 1996. Available from: https://ufdc.ufl.edu/AA00052899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

4. Feldstein, Judith Breiner, 1952-. Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat.

Degree: 1983, University of Florida

URL: https://ufdc.ufl.edu/AA00052894

Subjects/Keywords: Acinar cells; Bicarbonates; Enzymes; Erythrocytes; Gels; Kinetics; Molecular weight; pH; Rats; Saliva; Carbonic Anhydrases ( mesh ); Carbonic Anhydrases – analysis ( mesh ); Pharmacology and Therapeutics thesis Ph.D ( mesh ); Saliva – secretion ( mesh ); Salivary Glands ( mesh )

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APA (6^th Edition):

Feldstein, Judith Breiner, 1. (1983). Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00052894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Feldstein, Judith Breiner, 1952-. “Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat.” 1983. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00052894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Feldstein, Judith Breiner, 1952-. “Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat.” 1983. Web. 20 Jan 2021.

Vancouver:

Feldstein, Judith Breiner 1. Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat. [Internet] [Thesis]. University of Florida; 1983. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00052894.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Feldstein, Judith Breiner 1. Purification and characterization of carbonic anhydrase from the saliva and red cells of the rat. [Thesis]. University of Florida; 1983. Available from: https://ufdc.ufl.edu/AA00052894

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

5. Kararli, Tugrul T., 1956-. Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II.

Degree: 1984, University of Florida

URL: https://ufdc.ufl.edu/AA00004893

Subjects/Keywords: Active sites; Anions; Catalysis; Enzymes; Fluorescence; Imidazoles; Kinetics; pH; Protons; Sulfonamides; Carbonic Anhydrases ( mesh ); Carbonic anhydrase inhibitors ( mesh ); Pharmacology and Therapeutics thesis Ph.D ( mesh )

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APA (6^th Edition):

Kararli, Tugrul T., 1. (1984). Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00004893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kararli, Tugrul T., 1956-. “Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II.” 1984. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00004893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kararli, Tugrul T., 1956-. “Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II.” 1984. Web. 20 Jan 2021.

Vancouver:

Kararli, Tugrul T. 1. Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II. [Internet] [Thesis]. University of Florida; 1984. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00004893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kararli, Tugrul T. 1. Kinetics and inhibition of carbonic anhydrase III, comparison with carbonic anhydrase II. [Thesis]. University of Florida; 1984. Available from: https://ufdc.ufl.edu/AA00004893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tampere University

6. Yrjänäinen, Alma. Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi .

Degree: 2019, Tampere University

URL: https://trepo.tuni.fi/handle/10024/105797

► Background and aims of study: Carbonic anhydrase VI, CA VI, belongs to carbonic anhydrase isoenzymes that is known to secrete from serous acinar cells in… (more)

Subjects/Keywords: carbonic anhydrases; carbonic anhydrase VI; size-exclusion chromatography; static light scattering; dynamic light scattering

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APA (6^th Edition):

Yrjänäinen, A. (2019). Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi . (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/105797

Chicago Manual of Style (16^th Edition):

Yrjänäinen, Alma. “Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi .” 2019. Masters Thesis, Tampere University. Accessed January 20, 2021. https://trepo.tuni.fi/handle/10024/105797.

MLA Handbook (7^th Edition):

Yrjänäinen, Alma. “Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi .” 2019. Web. 20 Jan 2021.

Vancouver:

Yrjänäinen A. Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi . [Internet] [Masters thesis]. Tampere University; 2019. [cited 2021 Jan 20]. Available from: https://trepo.tuni.fi/handle/10024/105797.

Council of Science Editors:

Yrjänäinen A. Biochemical and biophysical characterization of saliva- and milk human carbonic anhydrase VI : maidon ja syljen hiilihappoanhydraasi VI:n biokemiallinen ja biofysikaalinen karakterisointi . [Masters Thesis]. Tampere University; 2019. Available from: https://trepo.tuni.fi/handle/10024/105797

Universidade Estadual de Campinas

7. Picco, Daniele de Cassia Rodrigues, 1981-. Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522

► Abstract: The carbonic anhydrase VI (CA VI) is the main enzyme responsible for maintaining the buffer capacity in the oral environment. There are no reports… (more)

▼ Abstract: The carbonic anhydrase VI (CA VI) is the main enzyme responsible for maintaining the buffer capacity in the oral environment. There are no reports in the literature on the relationship between the activity of this isoenzyme, pH, flow and buffering capacity and the prevalence of caries, and if the activity of CA VI can be employed as caries risk indicator. The objectives of this thesis were: 1) Conduct a systematic review of the literature 2) Determine the concentration and activity of CA VI in saliva and dental plaque of children with caries (C) and caries-free (CF) and the salivary flow, pH and buffering capacity; and 3) To investigate the relationship between these variables in saliva and dental plaque and tooth decay. For this, 74 students aged 7 to 9 years old were divided into 2 groups. It performed clinical examination, saliva collection and biofilm. The assays of the concentration of CA VI was performed by Elisa. The analysis of the CA VI activity by zymography. The salivary flow rate was expressed in ml/min. The salivary pH was determined by a portable pHmeter and the plaque pH with a selective electrode. The buffering capacity of saliva was measured eletrometrically. Data were subjected to Student's t test and Mann-Whitney, besides the Pearson and Spearman correlation analysis (? = 0.05). A bivariate analysis was performed in order to verify associations between the dependent and independent variables. In multivariate modeling, associations between the dependent and independent variables were expressed as odds ratios with 95% confidence intervals. The results showed significantly higher values of flow, salivar pH and buffering capacity in the CF group. The pH of the biofilm was also higher in the CF group. The concentration of CA VI in saliva and plaque were significantly higher in the LC group. The activity of CA VI was significantly higher in group C in saliva and plaque. There was a negative correlation between salivary buffering capacity and tooth decay. Moreover, a positive correlation was observed between the concentration and activity of CA VI in saliva and a negative correlation between the buffer capacity and isoenzyme activity in group C. In the CF group there was a negative correlation between pH and concentration of CA VI. A minor salivary flow and increased isoenzyme activity were significantly associated with caries. For dental plaque, it was observed negative correlation between activity and concentration of CA VI and between pH and activity of CA VI in the LC group. In the C group there was a negative correlation between pH and concentration of the CA VI. It concludes that the CA VI proved to be more active in saliva and dental plaque of children with active decay. Greater concentration of CA VI in saliva and dental plaque of caries-free children suggests that they are less exposed to cariogenic challenges, the system carbonate would be less active. The CA VI in saliva and dental plaque can be considered an anti-caries protein, as well as a biomarker for dental caries Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Nobre dos Santos, Marinês, 1956- (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Odontologia (nameofprogram), Rodrigues, Lidiany Karla Azevedo (committee member), Pessan, Juliano Pelim (committee member), Silva, Alan Roger dos Santos (committee member), Alarcon, Carolina Steiner Oliveira (committee member).

Subjects/Keywords: Anidrases carbônicas; Saliva; Placas dentárias; Cárie dentária; Carbonic anhydrases; Saliva; Dental plaque; Dental caries

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APA (6^th Edition):

Picco, Daniele de Cassia Rodrigues, 1. (2016). Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Picco, Daniele de Cassia Rodrigues, 1981-. “Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 20, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Picco, Daniele de Cassia Rodrigues, 1981-. “Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay.” 2016. Web. 20 Jan 2021.

Vancouver:

Picco, Daniele de Cassia Rodrigues 1. Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 20]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Picco, Daniele de Cassia Rodrigues 1. Estudo da concentração e atividade da anidrase carbônica VI na saliva e biofilme de crianças em idade escolar e sua relação com a cárie dentária = Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay: Study of concentration and activity of carbonic anhydrase VI in saliva and biofilm of children in school age and its relation to tooth decay. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/321522

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

8. Souza, Thayse Rodrigues de, 1987-. Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028

► Abstract: The carbonic anhydrase VI (CAVI) and ?-amylase (SAA) enzymes are present in saliva. AC VI is responsible for catalyzing the main reaction buffering the… (more)

▼ Abstract: The carbonic anhydrase VI (CAVI) and ?-amylase (SAA) enzymes are present in saliva. AC VI is responsible for catalyzing the main reaction buffering the oral cavity. SAA is associated with the pellicle and biofilms formation and starch metabolism. There are no reports in the literature that have longitudinally investigated the relationship between AC VI and dental caries and a cross-sectional study to investigate the SAA activity after a cariogenic challenge. The objectives of the Chapter 1 of this thesis were: Determine the stimulated salivary flow (SSFR), buffer capacity (BC) and CA VI activity in the saliva of children with caries and caries-free before and after rinsing with a sucrose solution to 20% and to investigate the relationship of these variables with dental caries in a longitudinal study of one year of follow-up. And of the Chapter 2: Investigate the SAA activity in saliva of children with caries and caries-free before and after rinsing with a sucrose solution at 20% and its relationship with SSFR, BC and dental caries in a cross-sectional study. Were allocated to the study of Chapter 1 47 children 48-78 months age, divided into three groups after calculation of caries increment after one year: caries free group (CF), caries lesion group (CL) and arrestment caries group (AC). And in Chapter 2, 38 children aging 48-77 months old, divided into two groups: caries lesion group (CL) and caries free group (CF). The activity of CA VI was quantified by zymography. The SSFR was expressed in mL/min. The BC was measured by Ericsson¿s method. The SAA activity was analyzed by the enzyme kinetic assay. Wilcoxon test and the Kruskal-Wallis test for paired comparisons of the values of CAVI before and after the rinses and comparison between groups respectively. To SSFR and BC data were employed the tests mentioned above in the two Chapters. The Student t test paired and independent were employed to the SAA data. It was also performed Spearman correlation analysis (? = 0.05). The results of chapter 1 show that CA VI activity significantly decreased after the cariogenic challenge at the CF group in baseline and follow-up and at AC group only at the follow-up (p= 0.037, p=0.028 e p=0.027, respectively). No change in CA VI activity was found at the two periods of the study in CL group. Salivary CA VI activity before rinse at the baseline shows also a negative correlation with dental caries at the baseline before and after rinse and at the follow-up before the rinse in the CL group (r=-0.609, p=0.004 e r=-0.516, p=0,020, r= -0.545, p=0.013, respectively). A negative correlation was found between dental caries at baseline as well at follow-up and BC after rinse at follow-up (r=-0.345, p=0.017 e r=-0.303, p=0.038 respectively). The results of Chapter 2 shows that the CL group exhibited a significant increase on SAA activity after rinse (p=0.001), and significantly differed from CF group (p=0.033). The results of the two Chapters show a significant increase and decrease of SSFR and BC respectively after the sucrose… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Nobre dos Santos, Marinês, 1956- (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Odontologia (nameofprogram), Delbem, Alberto Carlos Botazzo (committee member), Valarelli, Thais Marchini de Oliveira (committee member), Ferrua, Paula Midori Castelo (committee member), Leite, Michelle Franz Montan Braga (committee member).

Subjects/Keywords: Anidrases carbônicas; Alfa-amilases; Saliva; Cárie dentária; Carbonic anhydrases; Alpha-amylases; Saliva; Dental caries

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APA (6^th Edition):

Souza, Thayse Rodrigues de, 1. (2016). Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Souza, Thayse Rodrigues de, 1987-. “Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 20, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Souza, Thayse Rodrigues de, 1987-. “Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children.” 2016. Web. 20 Jan 2021.

Vancouver:

Souza, Thayse Rodrigues de 1. Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 20]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Souza, Thayse Rodrigues de 1. Relação entre a atividade da anidrase carbônica VI, alfa-amilase salivar, capacidade tampão, fluxo salivar e cárie dental em crianças = Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children: Relationship among salivary carbonic anhydrase VI activity, alpha-salivary amylase, buffering capacity, salivary flow rate and dental caries in children. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/325028

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Le Goff, Carine. Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum.

Degree: Docteur es, Sciences de la vie, 2016, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2016PA066439

►

Le processus de calcification chez Corallium rubrum conduit à la formation de deux structures squelettiques composées de CaCO3, l’axe squelettique et les sclérites, de taille… (more)

▼

Le processus de calcification chez Corallium rubrum conduit à la formation de deux structures squelettiques composées de CaCO3, l’axe squelettique et les sclérites, de taille et de forme différentes. Comme chez de nombreuses espèces calcifiantes, la calcification se fait sous contrôle biologique impliquant notamment des enzymes et des transporteurs ioniques. Une question centrale est d’identifier les mécanismes communs ou propres à chaque espèce qui sous-tendent leur convergence fonctionnelle envers ce processus. Deux approches ont été utilisées pour caractériser ces mécanismes chez C. rubrum: 1) Une approche physiologique avec le développement d’une technique de culture de microcolonies sur lamelles permettant d’observer différents stades de calcification, et de mesurer le pH aux sites de calcification par imagerie confocale ; 2) Une approche moléculaire afin de caractériser une famille d’enzymes, les anhydrases carboniques (ACs), qui jouent un rôle clef dans la calcification.Nous avons réalisé une cartographie du pH en effectuant des mesures dans différents compartiments intra- et extracellulaires. Nos résultats montrent notamment que le pH aux sites de calcification est supérieur à celui du milieu circulant dans les canaux gastrodermiques et non à celui l’eau de mer. Les mesures d’expression différentielle des ACs dans différents tissus mettent en évidence une isozyme préférentiellement exprimée dans les cellules calcifiantes.Ces résultats intégrés dans un contexte de calcification comparée pointent sur la convergence fonctionnelle des ACs et de la régulation du pH par les cellules calcifiantes, tout en soulignant des divergences évolutives.

The calcification process in Corallium rubrum leads to the formation of two skeletal structures made of calcium carbonate, the skeletal axis and sclerites, of different size and shape. As in many calcifying species, calcification occurs under a biological control that involves enzymes and ion transporters. A central issue is to determine the common and the species-specific mechanisms of calcification in order to identify functional convergences in this process. Two approaches were used to characterize these mechanisms in C. rubrum: 1) A physiological approach involving the development of a microcolony culture technique on glass coverslips, allowing the observation of the different stages of calcification, and the measurement of pH at the sites of calcification by the use of confocal microscopy; 2) A molecular approach to characterize an enzyme family, the carbonic anhydrases, which play a key role in calcification.We performed pH mapping by making measurements in different intra- and extracellular compartments. Our results show higher pH values at the sites of calcification compared with the fluid circulating in the gastrodermal canals, but not with the seawater surrounding the microcolony. Measurements of differential expression of carbonic anhydrases in different tissue fractions highlight an isozyme preferentially expressed in the calcifying cells.Within comparative…

Advisors/Committee Members: Tambutté, Sylvie (thesis director).

Subjects/Keywords: Corail; Corallium rubrum; Octocoralliaire; Calcification; Anhydrase carbonique; PH; Calcification; Corallium rubrum; Carbonic anhydrases; 571.1

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APA (6^th Edition):

Le Goff, C. (2016). Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066439

Chicago Manual of Style (16^th Edition):

Le Goff, Carine. “Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed January 20, 2021. http://www.theses.fr/2016PA066439.

MLA Handbook (7^th Edition):

Le Goff, Carine. “Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum.” 2016. Web. 20 Jan 2021.

Vancouver:

Le Goff C. Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2016PA066439.

Council of Science Editors:

Le Goff C. Approches physiologique et moléculaire de la calcification chez le corail rouge de méditerranée Corallium rubrum : Molecular and physiological approaches to study calcification in the mediterranean red coral Corallium rubrum. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066439

University of Edinburgh

10. Hughes, Sam. Investigating protein structure by top-down native protein mass spectrometry.

Degree: PhD, 2019, University of Edinburgh

URL: http://hdl.handle.net/1842/36627

► Determining protein structure is the central challenge of structural biology, owing to the integral role of proteins in biological systems along with the complexity and… (more)

▼ Determining protein structure is the central challenge of structural biology, owing to the integral role of proteins in biological systems along with the complexity and diversity of their structures. It is not only an important challenge, but a difficult one; the large size of proteins gives rise to a limitless range of potential structures, while the traditional methods of NMR and crystallography both carry specific limitations. Hence, there is a drive to develop new, orthogonal techniques that could provide additional insight into protein structure. Mass spectrometry (MS) is a ubiquitous analytical technique across all fields of chemistry, and the development of "soft" ionisation techniques has allowed noncovalent interactions to be retained in the gas phase and observed by MS. This in turn has led to the development of "native" MS in which proteins can be analysed and studied in the gas phase with their structures largely intact. Top-down MS of native proteins has been suggested as a potential avenue for studying protein structure. Here, the top-down technique of electron capture dissociation (ECD), which does not disrupt noncovalent interactions, is investigated for its potential to observe protein structure in native MS. Firstly, ECD was used to determine the sites at which a range of metal ions bind to the unstructured protein α-synuclein. These results were analysed through both a traditional top-down workflow and a novel mass defect based analysis. This mass defect analysis demonstrated how metal-containing fragments can detected without determination of their monoisotopic signal and shows potential to be applied in a range of top-down experiments. Secondly, ECD was investigated for its ability to probe the structure of native proteins. Carbonic anhydrase was used as a model protein, and it was observed that ECD can be used to monitor gas-phase unfolding pathways in much greater detail than otherwise available. However, studying multiple structural homologues showed that ECD is not exclusively directed by structure, but rather that availability of positive charge plays an equally important role in directing fragmentation. This was further investigated by producing K→A variants of carbonic anhydrase specifically designed to target ECD-sensitive regions of the protein. Our results demonstrate that minor differences to the primary sequence of a protein can significantly alter the observed ECD fragmentation patterns.

Subjects/Keywords: mass spectrometry; protein structure; carbonic anhydrases; electron capture dissociation; ECD; ECD fragmentation patterns

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APA (6^th Edition):

Hughes, S. (2019). Investigating protein structure by top-down native protein mass spectrometry. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/36627

Chicago Manual of Style (16^th Edition):

Hughes, Sam. “Investigating protein structure by top-down native protein mass spectrometry.” 2019. Doctoral Dissertation, University of Edinburgh. Accessed January 20, 2021. http://hdl.handle.net/1842/36627.

MLA Handbook (7^th Edition):

Hughes, Sam. “Investigating protein structure by top-down native protein mass spectrometry.” 2019. Web. 20 Jan 2021.

Vancouver:

Hughes S. Investigating protein structure by top-down native protein mass spectrometry. [Internet] [Doctoral dissertation]. University of Edinburgh; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1842/36627.

Council of Science Editors:

Hughes S. Investigating protein structure by top-down native protein mass spectrometry. [Doctoral Dissertation]. University of Edinburgh; 2019. Available from: http://hdl.handle.net/1842/36627

University of Oulu

11. Karhumaa, P. (Pepe). Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form.

Degree: 2002, University of Oulu

URL: http://urn.fi/urn:isbn:9514266641

► Abstract Carbonic anhydrases (CAs) are a group of zinc-containing metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate (CO2 + H2O ⇔ HCO3- +… (more)

▼ Abstract Carbonic anhydrases (CAs) are a group of zinc-containing metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate (CO2 + H2O ⇔ HCO3- + H+). They are present in almost all organs and are implicated in various biological functions, the most important of which is participation in the regulation of ion, water, and acid-base balance. Recently, some members of the CA gene family have been suggested to promote cell proliferation and to act as trophic growth factors. The present study was undertaken to examine the distribution of CA isoenzymes in the reproductive system, to attain a more detailed view on their linkage to the reproductive processes and to neonatal development. The expression of membrane-bound CA IX and CA XII was studied in the female and male reproductive tracts by immunohistochemistry and western blotting. CA XII was found to be expressed in the basolateral plasma membrane of luminal and glandular epithelia in human uterus. In human efferent ducts, it was located in the basolateral plasma membrane of luminal epithelium, where it coexpressed with Aquaporin-1. In epididymal duct, CA XII was only expressed in occasional epithelial cells. These cells coexpressed CA II, suggesting that they represent apical mitochondria-rich cells (AMRC). CA IX was also expressed in the basolateral plasma membrane of luminal epithelium in human efferent ducts, but its expression was not uniform among the tubules. These findings suggest that basolateral plasma membrane-associated CA IX and CA XII contribute, along with CA II and CA IV, to the regulation of acid-base balance and water transport in the reproductive tract. Western blotting of rat Leydig tumor cells and testis for CA II revealed an unidentified 66-kDa polypeptide band. The polypeptide was successfully purified from several rat tissues using CA inhibitor affinity chromatography. The amino acid sequence of the polypeptide showed it to be identical to NonO/p54nrb, a non-POU domain-containing octamer-binding protein previously implicated in transcriptional regulation. The recombinant NonO/p54nrb was shown to display CA activity, and the antibody to it predominantly immunostained the nuclei in lymphocytes, where CA activity was also detected histochemically. Accordingly, the nuclear Leydig cell CA immunoreactivity represents NonO/p54nrb. It is classified as a novel, nonclassical CA, and it may participate in pH-related events in the nucleus. Human and rat milk was found to contain CA VI by immunohistochemistry and western blotting. The enzyme purified from human milk by CA inhibitor affinity chromatography was confirmed by PNGase F digestion and amino acid sequence as CA VI. The CA VI concentrations in human colostral milk were approximately eight times higher than those in mature milk (34.7 mg/l vs. 4.5 mg/l). Secretion of CA VI into milk is suggested by its localization in the alveolar epithelium of the rat mammary gland. The structural and functional stability of CA VI in an acidic milieu, its suggested growth-supporting…

Subjects/Keywords: carbonic anhydrases; efferent ducts; milk; testis; uterus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Karhumaa, P. (. (2002). Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9514266641

Chicago Manual of Style (16^th Edition):

Karhumaa, P (Pepe). “Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form.” 2002. Doctoral Dissertation, University of Oulu. Accessed January 20, 2021. http://urn.fi/urn:isbn:9514266641.

MLA Handbook (7^th Edition):

Karhumaa, P (Pepe). “Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form.” 2002. Web. 20 Jan 2021.

Vancouver:

Karhumaa P(. Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form. [Internet] [Doctoral dissertation]. University of Oulu; 2002. [cited 2021 Jan 20]. Available from: http://urn.fi/urn:isbn:9514266641.

Council of Science Editors:

Karhumaa P(. Carbonic anhydrases in the reproductive system:with special emphasis on isoenzymes VI, IX, XII, and a novel nuclear nonclassical form. [Doctoral Dissertation]. University of Oulu; 2002. Available from: http://urn.fi/urn:isbn:9514266641

Wayne State University

12. Alsaab, Hashem Obaid. Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma.

Degree: PhD, Pharmaceutical Sciences, 2018, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/2002

► Renal Cell Carcinoma (RCC) contributes to more than 90% of the most common form of kidney tumor and remains one of the ten leading… (more)

▼ Renal Cell Carcinoma (RCC) contributes to more than 90% of the most common form of kidney tumor and remains one of the ten leading causes of cancer death in the United States. Although surgery remains an option for operable tumors, high metastatic index and resistance to radiation and chemotherapies prompted recent development of therapeutics that target the RCC angiogenesis and cell proliferation pathways. Tyrosine kinase inhibitors or TKIs (Cabozantinib, Axitinib, Sorafenib, and Sunitinib) and mammalian target of rapamycin (mTOR) inhibitors (Temsirolimus and Everolimus) have increased the therapeutic options for treating RCC. Although the impact towards decreasing disease progression is encouraging, a substantial proportion of patients do not respond adequately, and therapy resistance almost inevitably occurs. Eventually, new strategies with encouraging results have emerged that include immunotherapy, such as the programmed death-1 inhibitor (Nivolumab), cytokines, and a combination of chemo-immune therapy. Nevertheless, the 5-year disease free progression remains a meager <10% and these medicines produce fetal toxicity, contributing to poor overall prognosis. Thus, developing alternative strategies with effective treatment options remains an urgent unmet need for therapy-resistant RCC. In this regard, combination treatment targeting different cancer survival pathway of tumor microenvironment can be advantageous. Along these lines, we have pursued different combination drug regimens, including inhibitors that target mTOR (everolimus) and RTK or VEGFR (cabozantinib or sorafenib), as possible treatment strategies. In addition, we explored the rational design of nanoparticles to selectively deliver a variety of therapeutic payloads to target hypoxic tumor microenvironment overexpressing carbonic anhydrase-IX (CAIX). Our strategy involved establishing a library of tumor penetrating nanocarriers carrying combination drug payload of RTK-inhibitor with our own apoptosis inducer/CARP-1 protein functional mimetics (CFM-4.16). Nano-carriers were tailored to have varying composition and size that could: (i) efficiently reach the tumor core; (ii) target tumor multi-components including cancer epithelial cells and tumor-associated macrophages (TAM) for overcoming drug resistance in RCC. Specifically, the current work was focused on multimodal approaches, including (a) Optimization of hypoxia marker (CA IX) targeted polymer-lipid nano-formulation (PLNP) using copper-free ‘click’ chemistry; (b) In vitro and in vivo pre-clinical imaging and therapy of PLNP loaded with multiple drugs in inhibiting RCCs using mice bearing resistant RCCs and human cancer mimicking patient-derived xenografts (PDx). The results of antitumor efficacy and biodistribution of targeted PLNPs in animals bearing RCC xenograft and PDx models revealed selective accumulation of drugs at tumor sites resulting in greater tumor growth inhibition with reduced side effects. The highlights of the targeted formulation include specific tumor uptake,… Advisors/Committee Members: Arun K. Iyer.

Subjects/Keywords: angiogenesis; Carbonic Anhydrases IX; Hypoxia Targeting; Nanoformulation; Renal Cell Carcinoma; Tyrosine kinase inhibitors; Medicinal Chemistry and Pharmaceutics

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APA (6^th Edition):

Alsaab, H. O. (2018). Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/2002

Chicago Manual of Style (16^th Edition):

Alsaab, Hashem Obaid. “Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma.” 2018. Doctoral Dissertation, Wayne State University. Accessed January 20, 2021. https://digitalcommons.wayne.edu/oa_dissertations/2002.

MLA Handbook (7^th Edition):

Alsaab, Hashem Obaid. “Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma.” 2018. Web. 20 Jan 2021.

Vancouver:

Alsaab HO. Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma. [Internet] [Doctoral dissertation]. Wayne State University; 2018. [cited 2021 Jan 20]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/2002.

Council of Science Editors:

Alsaab HO. Tumor Multicomponent Targeting Polymer-Lipid Hybrid Nanoparticles To Overcome Drug Resistance In Renal Cell Carcinoma. [Doctoral Dissertation]. Wayne State University; 2018. Available from: https://digitalcommons.wayne.edu/oa_dissertations/2002

Tampere University

13. Isokangas, Lydia. Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases .

Degree: 2016, Tampere University

URL: https://trepo.tuni.fi/handle/10024/99277

► Background and Aims Humans are able to quickly recognize and evaluate visual patterns, thus this thesis aims to apply this feature to the analysis of… (more)

▼ Background and Aims Humans are able to quickly recognize and evaluate visual patterns, thus this thesis aims to apply this feature to the analysis of aspects of the conservation of carbonic anhydrase proteins. This was facilitated through the creation of two pipelines: * One to create a publically available specialized database to service the CA research world named CAbase, and, * One to create a visual display of the aligned exons of the cDNA transcripts contained within CAbase with indicators to show the positions of start and stop codons along with the locations of the predicted signal and mitochondrial targeting peptides. This pipeline was named Exon_Analysis. Carbonic anhydrases (CAs) are ubiquitous proteins that reversibly catalyse carbon dioxide into carbonic acid. Through the events of duplication, the CAs exist in at least 16 different isoforms and potentially up to 17 different isoforms. Methods The pipelines were created using freely available tools that included python, MySQL, various bioinformatic tools such as Clustal Omega, PRANK, BLAST and Pal2Nal. The data for CAbase was extracted from Ensembl, NCBI, UniProt, RSCB PDB, UniGene and FlyBase. Additionally, calculated data from using SignalP and TargetP was also included. CAbase is hosted on the Amazon Web Server and can be accessed using any computer that has access to the Internet and has MySQL installed. Exon_Analysis draws a scaled exon MSA schematic based on a PRANK MSA of the cDNA transcripts for a CA isoform. The exons and other indicators such as the start and stop codon, and the signal and target peptides are all drawn in different colours and in their scaled locations. Thus it is possible to see the conserved nature of the exons within the coding regions and the aligned start and stop codons and the peptides for each CA isoform. Results CAbase is now publically available for anyone to use. However, it is still somewhat user unfriendly due to the requirement that user be familiar with SQL. CAbase facilitated the use of Exon_Analysis. This pipeline has enabled the quality control of the predicted genes one exon at a time. Evolutionary events such as the conservation of short exons within CAs VI, IX, XII, XIV, X and XI has been shown in the exon MSA schematics. Both tools could be adapted for use with other proteins. Conclusion CAbase is a specialized database for CA researchers that can continue to be adapted to their needs. It allows researchers to create specific queries without having to filter for unwanted proteins. Exon_Analysis creates an exon MSA schematic to visualise the relationship between the exons and other features of interest. This facilitates the quality control of predicted genes one exon at a time.

Subjects/Keywords: carbonic anhydrases; specialised database; exon; ca; predicted genes quality check; signal peptide; mitochondrial targeting; exon analysis pipeline; visual assessment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Isokangas, L. (2016). Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases . (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/99277

Chicago Manual of Style (16^th Edition):

Isokangas, Lydia. “Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases .” 2016. Masters Thesis, Tampere University. Accessed January 20, 2021. https://trepo.tuni.fi/handle/10024/99277.

MLA Handbook (7^th Edition):

Isokangas, Lydia. “Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases .” 2016. Web. 20 Jan 2021.

Vancouver:

Isokangas L. Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases . [Internet] [Masters thesis]. Tampere University; 2016. [cited 2021 Jan 20]. Available from: https://trepo.tuni.fi/handle/10024/99277.

Council of Science Editors:

Isokangas L. Development of CAbase and an Exon Analysis Pipeline for Visual Assessment of Predicted Genes for the Carbonic Anhydrases . [Masters Thesis]. Tampere University; 2016. Available from: https://trepo.tuni.fi/handle/10024/99277

14. Canela, Heliara Maria Spina. Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus.

Degree: Mestrado, Biociências Aplicadas à Farmácia, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;

► O fungo Aspergillus fumigatus é o segundo maior causador de infecções fúngicas invasivas em pacientes imunocomprometidos e a principal espécie causadora da aspergilose invasiva, doença… (more)

▼ O fungo Aspergillus fumigatus é o segundo maior causador de infecções fúngicas invasivas em pacientes imunocomprometidos e a principal espécie causadora da aspergilose invasiva, doença de alta taxa de mortalidade que atinge principalmente os pulmões e que pode se disseminar pelo organismo. Durante o processo de infecção, o fungo precisa adaptar-se ao organismo do hospedeiro e um dos obstáculos encontrados é a mudança na concentração de dióxido de carbono (CO2), que, de 0,033% no ambiente, chega a até 6% no interior do hospedeiro. As anidrases carbônicas são enzimas envolvidas na hidratação reversível do CO2 e já foram apontadas como importantes na virulência de patógenos como Plasmodium falciparum, Mycobacterium tuberculosis, Helicobacter pylori, Cryptococcus neoformans e Candida albicans. Esse trabalho teve como objetivo avaliar o papel da enzima anidrase carbônica no desenvolvimento e virulência do fungo A. fumigatus, que apresenta quatro homólogos desta enzima (cafA, cafB, cafC e cafD). Para isso, foram utilizadas linhagens de A. fumigatus com os homólogos da enzima deletados (?cafA, ?cafB, ?cafC, ?cafD e ?cafA?cafB) e a linhagem selvagem (?akuBku80), da qual foram originadas as mutantes. Foram realizadas avaliações fenotípicas da estrutura dos conidióforos das diferentes linhagens, determinação da sensibilidade frente a diferentes agentes estressantes (antifúngicos, promotores de apoptose, estresse iônico, nitroativo, oxidativo, e de parede celular) e determinação da expressão gênica global em diferentes concentrações de CO2. Foi verificado que a deleção de cada um dos homólogos da anidrase carbônica de A. fumigatus não interfere na estrutura dos conidióforos deste fungo. Por outro lado, a deleção induziu alteração da sensibilidade do fungo frente a alguns compostos estressantes (ácido acético e peróxido de hidrogênio). Ainda, a análise da expressão gênica revelou um gene envolvido na adaptação do fungo ao aumento da concentração de CO2, o gene cipC, que não apresenta homólogos nas células de mamíferos. Este gene foi caracterizado neste trabalho por meio de sua deleção na linhagem selvagem (?akuBku80) de A. fumigatus e avaliação fenotípica microscópica e de sensibilidade a agentes estressantes (antifúngicos, promotores de apoptose, estresse iônico, nitroativo, oxidativo, e de parede celular). A deleção do gene não interferiu na estrutura do fungo, porém aumentou sua sensibilidade a alguns compostos (calcoflúor e menadiona). Foram realizados, ainda, testes de virulência em modelo animal utilizando-se o mutante ?cipC, os quais revelaram que a deleção deste gene atenua a virulência do fungo. Assim, foi possível concluir que as anidrases carbônicas não são relevantes para o desenvolvimento e virulência de A. fumigatus; porém, este fungo modifica a expressão de seus genes de modo a adaptar-se às variações na concentração atmosférica de CO2. O gene cipC está envolvido nesse processo de adaptação e é importante para o desenvolvimento do fungo e sua virulência, tornando-se um alvo para o estudo de novas terapias para o… Advisors/Committee Members: Balieiro, Marcia Eliana da Silva Ferreira.

Subjects/Keywords: Análise da expressão gênica; Análise da virulência; Anidrases carbônicas; Aspergillus fumigatus; Aspergillus fumigatus; Aspergilose invasiva; Carbonic anhydrases; cipC; cipC; Genic expression analysis; Invasive aspergillosis; Virulence analysis

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APA (6^th Edition):

Canela, H. M. S. (2013). Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;

Chicago Manual of Style (16^th Edition):

Canela, Heliara Maria Spina. “Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus.” 2013. Masters Thesis, University of São Paulo. Accessed January 20, 2021. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;.

MLA Handbook (7^th Edition):

Canela, Heliara Maria Spina. “Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus.” 2013. Web. 20 Jan 2021.

Vancouver:

Canela HMS. Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 20]. Available from: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;.

Council of Science Editors:

Canela HMS. Análise molecular da anidrase carbônica no fungo patogênico humano Aspergillus fumigatus. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/60/60135/tde-29012014-091548/ ;

Louisiana State University

15. Ynalvez, Ruby Agno. Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii.

Degree: PhD, 2007, Louisiana State University

URL: etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778

► Aquatic photosynthetic organisms such as the green alga, C. reinhardtii respond to low carbon dioxide conditions by inducing a carbon dioxide concentrating mechanism (CCM). Important… (more)

Subjects/Keywords: carbon dioxide concentrating mechanism; carbonic anhydrases; insertional mutant; Chlamydomonas reinhardtii

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APA (6^th Edition):

Ynalvez, R. A. (2007). Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778

Chicago Manual of Style (16^th Edition):

Ynalvez, Ruby Agno. “Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii.” 2007. Doctoral Dissertation, Louisiana State University. Accessed January 20, 2021. etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778.

MLA Handbook (7^th Edition):

Ynalvez, Ruby Agno. “Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii.” 2007. Web. 20 Jan 2021.

Vancouver:

Ynalvez RA. Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii. [Internet] [Doctoral dissertation]. Louisiana State University; 2007. [cited 2021 Jan 20]. Available from: etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778.

Council of Science Editors:

Ynalvez RA. Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii. [Doctoral Dissertation]. Louisiana State University; 2007. Available from: etd-07052007-000931 ; https://digitalcommons.lsu.edu/gradschool_dissertations/778

University of Oklahoma

16. Culver, Billy Jr. Impact of environmental (pH) change in a model aquatic herbivore: from genes to populations.

Degree: PhD, 2016, University of Oklahoma

URL: http://hdl.handle.net/11244/34612

► Organisms are subjected to a variety of environmental stressors in which they must respond in order to survive and reproduce. While some individuals are able… (more)

▼ Organisms are subjected to a variety of environmental stressors in which they must respond in order to survive and reproduce. While some individuals are able to adjust to these stressors and live to produce offspring and propagate their genes, others do not and are extirpated. Although it is known that organisms can respond to environmental stress, the underlying physiological and genetic mechanisms are often not well understood. Elucidating the evolutionary responses of organisms to environmental gradients is important, especially in light of increasing anthropogenic changes to our environment. In this dissertation, I looked at the acidification and alkalization of three North American lakes (Frenchman, Hill, Madison). In particular, I was interested in the underlying genetic response (evolution) of populations of the keystone aquatic zooplankter, Daphnia pulicaria, to the pH gradient found in these three lakes. In Chapter one, I used ecological genetic tools to determine local adaptation of the model organism, D. pulicaria, across a pH gradient in three North American lakes. I predicted there would be genetic differentiation and local adaptation among the three Daphnia populations. I genotyped individuals, which were used to determine genetic structure of the three populations. To test for signatures of local adaptation, a survivorship experiment across a pH gradient under common garden conditions was performed. In Chapter two, I was interested in determining candidate genes that may be involved in acid-base regulation in D. pulicaria. Previous studies have shown that carbonic anhydrases (CAs), a family of zinc metallo-enzymes, are responsible for acid-base regulation in many organisms. Through the use of phylogenetic tools, Chapter two attempted to find homologous CA isoforms in Daphnia that are implicated in acid-base regulation in closely related aquatic taxa. In Chapter three, I characterized the three isoforms of α-CAs found in Chapter two (CA1, CA2, and CA5). In addition, under common garden conditions, I investigated the differential expression of those CAs from D. pulicaria clones isolated from three North American lakes that exhibit a pH gradient. Finally, in Chapter four, I investigated the processes which affect genetic variation: neutral processes (i.e. genetic drift) versus natural selection (i.e. positive, purifying selection). I predicted that there will be evidence of selection at variants of these three CA loci and that specific CA genotypes will convey a fitness advantage via differential survivorship across a pH gradient. Populations were analyzed using population genetic tools. Further, five distinct CA genotypes were chosen for a common garden pH survival experiment to determine differential survivorship across a pH gradient. In summary, I identified three CAs that were homologous to CAs found to be implicated in acid-base regulation in other aquatic organisms. These isoforms were well-conserved across taxa and I found evidence that CA1 was differentially-expressed across a pH… Advisors/Committee Members: Weider, Lawrence (advisor), Broughton, Richard (committee member), Cichewicz, Robert (committee member), Hambright, David (committee member), Schlupp, Ingo (committee member).

Subjects/Keywords: acid-base regulation; Daphnia; ecological genetics; carbonic anhydrase

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APA (6^th Edition):

Culver, B. J. (2016). Impact of environmental (pH) change in a model aquatic herbivore: from genes to populations. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/34612

Chicago Manual of Style (16^th Edition):

Culver, Billy Jr. “Impact of environmental (pH) change in a model aquatic herbivore: from genes to populations.” 2016. Doctoral Dissertation, University of Oklahoma. Accessed January 20, 2021. http://hdl.handle.net/11244/34612.

MLA Handbook (7^th Edition):

Culver, Billy Jr. “Impact of environmental (pH) change in a model aquatic herbivore: from genes to populations.” 2016. Web. 20 Jan 2021.

Vancouver:

Culver BJ. Impact of environmental (pH) change in a model aquatic herbivore: from genes to populations. [Internet] [Doctoral dissertation]. University of Oklahoma; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11244/34612.

Council of Science Editors:

Culver BJ. Impact of environmental (pH) change in a model aquatic herbivore: from genes to populations. [Doctoral Dissertation]. University of Oklahoma; 2016. Available from: http://hdl.handle.net/11244/34612

Michigan State University

17. Yoshihara, Corinne Misae. The nucleotide sequence of the chicken carbonic anhydrase II gene.

Degree: PhD, Genetics Interdisciplinary Program, 1986, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:37104

Bibliographies throughout

Subjects/Keywords: Chickens – Genetics; Nucleotide sequence; Carbonic anhydrase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yoshihara, C. M. (1986). The nucleotide sequence of the chicken carbonic anhydrase II gene. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:37104

Chicago Manual of Style (16^th Edition):

Yoshihara, Corinne Misae. “The nucleotide sequence of the chicken carbonic anhydrase II gene.” 1986. Doctoral Dissertation, Michigan State University. Accessed January 20, 2021. http://etd.lib.msu.edu/islandora/object/etd:37104.

MLA Handbook (7^th Edition):

Yoshihara, Corinne Misae. “The nucleotide sequence of the chicken carbonic anhydrase II gene.” 1986. Web. 20 Jan 2021.

Vancouver:

Yoshihara CM. The nucleotide sequence of the chicken carbonic anhydrase II gene. [Internet] [Doctoral dissertation]. Michigan State University; 1986. [cited 2021 Jan 20]. Available from: http://etd.lib.msu.edu/islandora/object/etd:37104.

Council of Science Editors:

Yoshihara CM. The nucleotide sequence of the chicken carbonic anhydrase II gene. [Doctoral Dissertation]. Michigan State University; 1986. Available from: http://etd.lib.msu.edu/islandora/object/etd:37104

University of North Texas

18. Hoang, Chau V. Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis.

Degree: 2001, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc2893/

► Recently, plastidial carbonic anhydrase (CA, EC 4.2.1.1) cDNA clones encoding functional CA enzymes were isolated from a nonphotosynthetic cotton tissue. The role of CA in… (more)

▼ Recently, plastidial carbonic anhydrase (CA, EC 4.2.1.1) cDNA clones encoding functional CA enzymes were isolated from a nonphotosynthetic cotton tissue. The role of CA in photosynthetic tissues have been well characterized, however there is almost no information for the role of CA in nonphotosynthetic tissues. A survey of relative CA transcript abundance and enzyme activity in different cotton organs revealed that there was substantial CA expression in cotyledons of seedlings and embryos, both nonphotosynthetic tissues. To gain insight into the role(s) of CA, I examined CA expression in cotyledons of seedlings during post-germinative growth at different environmental conditions. CA expression in cotyledons of seedlings increased from 18 h to 72 h after germination in the dark. Seedlings exposed to light had about a 2-fold increase in CA activities when compared with seedlings kept in the dark, whereas relative CA transcript levels were essentially the same. Manipulation of external CO2 environments [zero, ambient (350 ppm), or high (1000 ppm)] modulated coordinately the relative transcript abundance of CA (and rbcS) in cotyledons, but did not affect enzyme activities. On the other hand, regardless of the external CO2 conditions seedlings exposed to light exhibited increase CA activity, concomitant with Rubisco activity and increased chlorophyll content. Our data revealed that steady-state levels of CA and rbcS transcripts are regulated at the transcriptional level in response to external CO2 conditions, while CA and Rubisco activities are modulated at the post-transcriptional level by light. Thus CA expression in cotyledons during post-germinative growth may be to “prime” cotyledons for the transition at the subcellular level for the transition from plastids to chloroplasts, where it provides CO2 for Rubisco during photosynthesis. Furthermore, CA expression increased during embryo maturation similar to oil accumulation. Specific sulfonamide inhibitors of CA activity significantly reduced the rate of [14C]-acetate incorporation into total lipids in cotton embryos and tobacco leaves and cell suspensions in vivo and in vitro. Similar results were obtained in chloroplasts isolated from leaves of transgenic CA antisense-suppressed tobacco plants (5% of wildtype activity). Collectively, these results support the notion that CA plays several physiological roles in nonphotosynthetic tissues. Advisors/Committee Members: Chapman, Kent D., Pirtle, Robert M., Benjamin, Robert C., Knesek, John, Root, Douglas D..

Subjects/Keywords: Carbonic anhydrase.; Cotton – Genetics.; Carbonic anhydrase enzymes; nonphotosynthetic tissue; post-germinative growth

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APA (6^th Edition):

Hoang, C. V. (2001). Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc2893/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hoang, Chau V. “Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis.” 2001. Thesis, University of North Texas. Accessed January 20, 2021. https://digital.library.unt.edu/ark:/67531/metadc2893/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hoang, Chau V. “Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis.” 2001. Web. 20 Jan 2021.

Vancouver:

Hoang CV. Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis. [Internet] [Thesis]. University of North Texas; 2001. [cited 2021 Jan 20]. Available from: https://digital.library.unt.edu/ark:/67531/metadc2893/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hoang CV. Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis. [Thesis]. University of North Texas; 2001. Available from: https://digital.library.unt.edu/ark:/67531/metadc2893/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Bertucci, Anthony. Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797).

Degree: Docteur es, Océanographie, 2010, Aix-Marseille 2

URL: http://www.theses.fr/2010AIX22112

►

La formation d’un squelette de CaCO3 par les coraux Scléractiniaires est à la base de l’édification des récifs coralliens. Nombre de ces coraux constructeurs de… (more)

▼

La formation d’un squelette de CaCO3 par les coraux Scléractiniaires est à la base de l’édification des récifs coralliens. Nombre de ces coraux constructeurs de récif vivent en symbiose avec des Dinoflagellés photosynthétiques. Ces deux processus reposent sur le transport et l’utilisation de carbone inorganique (Ci) provenant de l’eau de mer pour la photosynthèse, et du métabolisme animal pour la calcification. Cette thèse s’est intéressée à l’étude moléculaire et physiologique des mécanismes, permettant l’utilisation de ce carbone inorganique.Malgré l’importance des transports de HCO3-, aucun transporteur n’a été caractérisé à cejour et leur implication dans la physiologie des coraux n’est que suggérée par la pharmacologie. Durant cette thèse nous avons cloné un gène codant pour un transporteur deHCO3- chez le corail Acropora sp. La conversion de ce HCO3- en CO2 pour la photosynthèse est facilitée par l’acidification de l’environnement proche du Dinoflagellé dans la cellule animale. Cette acidification est causée par une H+-ATPase de type P que nous avons caractérisée. Ce gène est le premier à montrer une expression dépendante de la vie en symbiose chez le symbiote.Nous avons aussi cloné et localisé deux anhydrases carboniques (AC). L’une impliquée dans la calcification et l’autre dans la régulation du pH intracellulaire et l’équilibre entre leCO2 et HCO3-. Une étude pharmacologique de ces deux AC, a identifié des molécules inhibitrices et activatrices qui ont permis des expériences de physiologie in vivo. Celles-ci permettent une analyse plus discriminante du rôle des AC dans la calcification.

Coral reefs edification is based on the formation of a calcium carbonate skeleton byscleractinian corals. Many of these reef-building corals establish a symbiotic association with photosynthetic Dinoflagellates. Both processes involve the transport and utilization of inorganic carbon (Ci) coming from seawater for photosynthesis, and from animal metabolismfor calcification. This work focused on the molecular and physiological study of poorlyknown mechanisms that allow the utilization of Ci.Despite the importance of bicarbonate transport, no transporter has been characterized and their role in coral physiology is only suggested by pharmacological experiments. We have cloned a gene encoding a bicarbonate transporter in the coral Acropora sp. The conversion of this bicarbonate into CO2 for photosynthesis is mediated by the acidification of the are asurrounding the Dinoflagellate in the animal cell. This is performed by a P type H+-ATPasethat we characterized here. This is the first gene with a symbiosis-dependent expression in the symbiont.This work also allowed the cloning and the localization of two carbonic anhydrases (CA).The first one is involved in calcification, the second one plays a role in the intracellular pHregulation and the CO2 / HCO3- equilibrium. A pharmacological study of these two enzymes identified inhibitor and activator compounds that have been then used in physiology experiments. This last approach…

Advisors/Committee Members: Allemand, Denis (thesis director), Zoccola, Didier (thesis director).

Subjects/Keywords: Scléractiniaires; Stylophora pistillata; Carbone inorganique; Biominéralisation; Mécanismes de concentrations du CO2; Anhydrases carboniques; Scleractinian; Stylophora pistillata; Inorganic carbon; Biomineralization; CO2- concentrating-mechanism; Carbonic anhydrase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bertucci, A. (2010). Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797). (Doctoral Dissertation). Aix-Marseille 2. Retrieved from http://www.theses.fr/2010AIX22112

Chicago Manual of Style (16^th Edition):

Bertucci, Anthony. “Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797).” 2010. Doctoral Dissertation, Aix-Marseille 2. Accessed January 20, 2021. http://www.theses.fr/2010AIX22112.

MLA Handbook (7^th Edition):

Bertucci, Anthony. “Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797).” 2010. Web. 20 Jan 2021.

Vancouver:

Bertucci A. Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797). [Internet] [Doctoral dissertation]. Aix-Marseille 2; 2010. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2010AIX22112.

Council of Science Editors:

Bertucci A. Etudes moléculaire et physiologique des mécanismes permettant l'utilisation du carbone inorganique chez le corail Scléractiniaire Stylophora pistillata (Esper, 1797) : Molecular and physiological studies of inorganic carbon utilization mechanisms in the scleractinian coral Stylophora pistillata (Esper, 1797). [Doctoral Dissertation]. Aix-Marseille 2; 2010. Available from: http://www.theses.fr/2010AIX22112

University of North Texas

20. Local, Andrea. Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.).

Degree: 1998, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc279044/

► Carbonic anhydrase is a ubiquitous zinc-metalloenzyme that catalyzes the interconversion of carbon dioxide and carbonate and has been found to play a wide range of… (more)

Subjects/Keywords: cotton; nucleotide sequences; genetics; Carbonic anhydrase.; Nucleotide sequence.; Molecular cloning.; Cotton – Genetics.

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APA (6^th Edition):

Local, A. (1998). Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.). (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc279044/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Local, Andrea. “Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.).” 1998. Thesis, University of North Texas. Accessed January 20, 2021. https://digital.library.unt.edu/ark:/67531/metadc279044/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Local, Andrea. “Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.).” 1998. Web. 20 Jan 2021.

Vancouver:

Local A. Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.). [Internet] [Thesis]. University of North Texas; 1998. [cited 2021 Jan 20]. Available from: https://digital.library.unt.edu/ark:/67531/metadc279044/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Local A. Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.). [Thesis]. University of North Texas; 1998. Available from: https://digital.library.unt.edu/ark:/67531/metadc279044/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

21. Oberle, Suzan Marie, 1963-. Cloning, expression, and immune recognition of the conserved MSP4 protein of Anaplasma marginale.

Degree: 1993, University of Florida

URL: https://ufdc.ufl.edu/AA00066663

Subjects/Keywords: Research ( mesh ); Anaplasma – immunology ( mesh ); Anaplasma – genetics ( mesh ); Cloning, Molecular ( mesh ); Gene Expression ( mesh ); Genes, Bacterial ( mesh ); Cattle – immunology ( mesh ); Membrane Proteins – immunology ( mesh ); Membrane Proteins – genetics ( mesh ); Conserved Sequence ( mesh ); Immunologic Tests ( mesh ); Molecular Sequence Data ( mesh ); Base Sequence ( mesh ); Amino Acid Sequence ( mesh ); Department of Infectious Diseases thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oberle, Suzan Marie, 1. (1993). Cloning, expression, and immune recognition of the conserved MSP4 protein of Anaplasma marginale. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00066663

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oberle, Suzan Marie, 1963-. “Cloning, expression, and immune recognition of the conserved MSP4 protein of Anaplasma marginale.” 1993. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00066663.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oberle, Suzan Marie, 1963-. “Cloning, expression, and immune recognition of the conserved MSP4 protein of Anaplasma marginale.” 1993. Web. 20 Jan 2021.

Vancouver:

Oberle, Suzan Marie 1. Cloning, expression, and immune recognition of the conserved MSP4 protein of Anaplasma marginale. [Internet] [Thesis]. University of Florida; 1993. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00066663.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oberle, Suzan Marie 1. Cloning, expression, and immune recognition of the conserved MSP4 protein of Anaplasma marginale. [Thesis]. University of Florida; 1993. Available from: https://ufdc.ufl.edu/AA00066663

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

22. III Grimes, H. Leighton, 1961-. Genomic structure, expression and alternative splicing of the ski proto-oncogene.

Degree: 1991, University of Florida

URL: https://ufdc.ufl.edu/AA00065781

Subjects/Keywords: Research ( mesh ); Proto-Oncogenes – genetics ( mesh ); Alternative Splicing ( mesh ); Gene Expression Regulation, Viral ( mesh ); Gene Expression ( mesh ); Genome, Viral ( mesh ); Retroviridae – genetics ( mesh ); Retroviridae Proteins ( mesh ); Department of Pathology and Laboratory Medicine thesis Ph.D ( mesh )

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APA (6^th Edition):

III Grimes, H. Leighton, 1. (1991). Genomic structure, expression and alternative splicing of the ski proto-oncogene. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00065781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

III Grimes, H. Leighton, 1961-. “Genomic structure, expression and alternative splicing of the ski proto-oncogene.” 1991. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00065781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

III Grimes, H. Leighton, 1961-. “Genomic structure, expression and alternative splicing of the ski proto-oncogene.” 1991. Web. 20 Jan 2021.

Vancouver:

III Grimes, H. Leighton 1. Genomic structure, expression and alternative splicing of the ski proto-oncogene. [Internet] [Thesis]. University of Florida; 1991. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00065781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

III Grimes, H. Leighton 1. Genomic structure, expression and alternative splicing of the ski proto-oncogene. [Thesis]. University of Florida; 1991. Available from: https://ufdc.ufl.edu/AA00065781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

23. Viseshakul, Nareerat, 1960-. Cloning and expression of the msp1 beta multigene family of Anaplasma marginale.

Degree: 1994, University of Florida

URL: https://ufdc.ufl.edu/AA00067429

Subjects/Keywords: Research ( mesh ); Anaplasma – genetics ( mesh ); Cloning, Molecular ( mesh ); Gene Expression ( mesh ); Multigene Family – genetics ( mesh ); Molecular Sequence Data ( mesh ); Base Sequence ( mesh ); Amino Acid Sequence ( mesh ); Department of Infectious Diseases thesis Ph.D ( mesh )

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APA (6^th Edition):

Viseshakul, Nareerat, 1. (1994). Cloning and expression of the msp1 beta multigene family of Anaplasma marginale. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00067429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Viseshakul, Nareerat, 1960-. “Cloning and expression of the msp1 beta multigene family of Anaplasma marginale.” 1994. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00067429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Viseshakul, Nareerat, 1960-. “Cloning and expression of the msp1 beta multigene family of Anaplasma marginale.” 1994. Web. 20 Jan 2021.

Vancouver:

Viseshakul, Nareerat 1. Cloning and expression of the msp1 beta multigene family of Anaplasma marginale. [Internet] [Thesis]. University of Florida; 1994. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00067429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Viseshakul, Nareerat 1. Cloning and expression of the msp1 beta multigene family of Anaplasma marginale. [Thesis]. University of Florida; 1994. Available from: https://ufdc.ufl.edu/AA00067429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

24. Lepine, Guylaine, 1965-. A Molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis.

Degree: 1994, University of Florida

URL: https://ufdc.ufl.edu/AA00067568

Subjects/Keywords: Research ( mesh ); Hemagglutinins – genetics ( mesh ); Genes, Bacterial – genetics ( mesh ); Gene Expression ( mesh ); Porphyromonas gingivalis ( mesh ); Molecular Sequence Data ( mesh ); Base Sequence ( mesh ); Amino Acid Sequence ( mesh ); Department of Oral Biology thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lepine, Guylaine, 1. (1994). A Molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00067568

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lepine, Guylaine, 1965-. “A Molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis.” 1994. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00067568.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lepine, Guylaine, 1965-. “A Molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis.” 1994. Web. 20 Jan 2021.

Vancouver:

Lepine, Guylaine 1. A Molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis. [Internet] [Thesis]. University of Florida; 1994. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00067568.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lepine, Guylaine 1. A Molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis. [Thesis]. University of Florida; 1994. Available from: https://ufdc.ufl.edu/AA00067568

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

25. Cao, Xuefang, 1970-. Cleavage of Bax to Pis Bax facilitates stress-induced apoptosis.

Degree: 2003, University of Florida

URL: https://ufdc.ufl.edu/AA00067617

Subjects/Keywords: Apoptosis – genetics ( mesh ); Apoptosis – drug effects ( mesh ); Neoplasms – genetics ( mesh ); Calpain ( mesh ); Research ( mesh ); Department of Pharmacology and Therapeutics thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cao, Xuefang, 1. (2003). Cleavage of Bax to Pis Bax facilitates stress-induced apoptosis. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00067617

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cao, Xuefang, 1970-. “Cleavage of Bax to Pis Bax facilitates stress-induced apoptosis.” 2003. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00067617.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cao, Xuefang, 1970-. “Cleavage of Bax to Pis Bax facilitates stress-induced apoptosis.” 2003. Web. 20 Jan 2021.

Vancouver:

Cao, Xuefang 1. Cleavage of Bax to Pis Bax facilitates stress-induced apoptosis. [Internet] [Thesis]. University of Florida; 2003. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00067617.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cao, Xuefang 1. Cleavage of Bax to Pis Bax facilitates stress-induced apoptosis. [Thesis]. University of Florida; 2003. Available from: https://ufdc.ufl.edu/AA00067617

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

26. Van Ginkel, Paul R., 1962-. Developmental expression of bovine rod and cone photoreceptor-specific genes.

Degree: 1994, University of Florida

URL: https://ufdc.ufl.edu/AA00067428

Subjects/Keywords: Research ( mesh ); Retinal Pigments – genetics ( mesh ); Photoreceptors – genetics ( mesh ); Gene Expression Regulation ( mesh ); Gene Expression ( mesh ); Enzyme Induction ( mesh ); Transcription, Genetic ( mesh ); Opsin ( mesh ); Retina – embryology ( mesh ); Cattle ( mesh ); Molecular Sequence Data ( mesh ); Base Sequence ( mesh ); Amino Acid Sequence ( mesh ); Department of Immunology and Medical Microbiology thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Ginkel, Paul R., 1. (1994). Developmental expression of bovine rod and cone photoreceptor-specific genes. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00067428

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van Ginkel, Paul R., 1962-. “Developmental expression of bovine rod and cone photoreceptor-specific genes.” 1994. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00067428.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van Ginkel, Paul R., 1962-. “Developmental expression of bovine rod and cone photoreceptor-specific genes.” 1994. Web. 20 Jan 2021.

Vancouver:

Van Ginkel, Paul R. 1. Developmental expression of bovine rod and cone photoreceptor-specific genes. [Internet] [Thesis]. University of Florida; 1994. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00067428.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van Ginkel, Paul R. 1. Developmental expression of bovine rod and cone photoreceptor-specific genes. [Thesis]. University of Florida; 1994. Available from: https://ufdc.ufl.edu/AA00067428

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

27. Zhu, Hao, 1971-. Development of serum stable lipoplex for in vivo gene transfer and characterization of coformulation liposomes for increased gene expression.

Degree: 2004, University of Florida

URL: https://ufdc.ufl.edu/AA00067654

Subjects/Keywords: Liposomes – genetics ( mesh ); Gene Transfer Techniques ( mesh ); Brain Neoplasms ( mesh ); Rats – physiopathology ( mesh ); Department of Pharmaceutics thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhu, Hao, 1. (2004). Development of serum stable lipoplex for in vivo gene transfer and characterization of coformulation liposomes for increased gene expression. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00067654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhu, Hao, 1971-. “Development of serum stable lipoplex for in vivo gene transfer and characterization of coformulation liposomes for increased gene expression.” 2004. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00067654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhu, Hao, 1971-. “Development of serum stable lipoplex for in vivo gene transfer and characterization of coformulation liposomes for increased gene expression.” 2004. Web. 20 Jan 2021.

Vancouver:

Zhu, Hao 1. Development of serum stable lipoplex for in vivo gene transfer and characterization of coformulation liposomes for increased gene expression. [Internet] [Thesis]. University of Florida; 2004. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00067654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhu, Hao 1. Development of serum stable lipoplex for in vivo gene transfer and characterization of coformulation liposomes for increased gene expression. [Thesis]. University of Florida; 2004. Available from: https://ufdc.ufl.edu/AA00067654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

28. Bueno Trujillo, Lina Clemencia. Characterization of antibiotic resistance determinants of Prevotella intermedia.

Degree: 1997, University of Florida

URL: https://ufdc.ufl.edu/AA00063497

Subjects/Keywords: Research ( mesh ); Prevotella intermedia – pathogenicity ( mesh ); Prevotella intermedia – genetics ( mesh ); Bacteroidaceae Infections ( mesh ); Drug Resistance, Microbial – genetics ( mesh ); Penicillinase ( mesh ); Tetracycline Resistance – genetics ( mesh ); Penicillin Resistance – genetics ( mesh ); Lactobacillus ( mesh ); Bacteroides ( mesh ); Department of Oral Biology thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bueno Trujillo, L. C. (1997). Characterization of antibiotic resistance determinants of Prevotella intermedia. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00063497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bueno Trujillo, Lina Clemencia. “Characterization of antibiotic resistance determinants of Prevotella intermedia.” 1997. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00063497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bueno Trujillo, Lina Clemencia. “Characterization of antibiotic resistance determinants of Prevotella intermedia.” 1997. Web. 20 Jan 2021.

Vancouver:

Bueno Trujillo LC. Characterization of antibiotic resistance determinants of Prevotella intermedia. [Internet] [Thesis]. University of Florida; 1997. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00063497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bueno Trujillo LC. Characterization of antibiotic resistance determinants of Prevotella intermedia. [Thesis]. University of Florida; 1997. Available from: https://ufdc.ufl.edu/AA00063497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

29. Muise, Robin Carol, 1961-. Regulation of RNA levels in plant mitochondria.

Degree: 1991, University of Florida

URL: https://ufdc.ufl.edu/AA00065789

Subjects/Keywords: Research ( mesh ); Transcription, Genetic ( mesh ); Mitochondria ( mesh ); RNA ( mesh ); Zea mays – genetics ( mesh ); Gene Expression Regulation, Plant ( mesh ); Brassica – genetics ( mesh ); Department of Immunology and Medical Microbiology thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Muise, Robin Carol, 1. (1991). Regulation of RNA levels in plant mitochondria. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00065789

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Muise, Robin Carol, 1961-. “Regulation of RNA levels in plant mitochondria.” 1991. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00065789.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Muise, Robin Carol, 1961-. “Regulation of RNA levels in plant mitochondria.” 1991. Web. 20 Jan 2021.

Vancouver:

Muise, Robin Carol 1. Regulation of RNA levels in plant mitochondria. [Internet] [Thesis]. University of Florida; 1991. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00065789.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Muise, Robin Carol 1. Regulation of RNA levels in plant mitochondria. [Thesis]. University of Florida; 1991. Available from: https://ufdc.ufl.edu/AA00065789

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

30. Yaswen, Linda Ruth. Cloning and characterization of Epstein-Barr virus glycoprotein gp85.

Degree: 1993, University of Florida

URL: https://ufdc.ufl.edu/AA00066674

Subjects/Keywords: Research ( mesh ); Herpesvirus 4, Human ( mesh ); Viral Envelope Proteins – genetics ( mesh ); Viral Envelope Proteins – chemistry ( mesh ); Membrane Proteins – genetics ( mesh ); Membrane Proteins – chemistry ( mesh ); Glycoproteins – genetics ( mesh ); Glycoproteins – chemistry ( mesh ); Molecular Sequence Data ( mesh ); Amino Acid Sequence ( mesh ); Department of Pathology and Laboratory Medicine thesis Ph.D ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yaswen, L. R. (1993). Cloning and characterization of Epstein-Barr virus glycoprotein gp85. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00066674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yaswen, Linda Ruth. “Cloning and characterization of Epstein-Barr virus glycoprotein gp85.” 1993. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00066674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yaswen, Linda Ruth. “Cloning and characterization of Epstein-Barr virus glycoprotein gp85.” 1993. Web. 20 Jan 2021.

Vancouver:

Yaswen LR. Cloning and characterization of Epstein-Barr virus glycoprotein gp85. [Internet] [Thesis]. University of Florida; 1993. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00066674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yaswen LR. Cloning and characterization of Epstein-Barr virus glycoprotein gp85. [Thesis]. University of Florida; 1993. Available from: https://ufdc.ufl.edu/AA00066674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations