subject:(Canine pyoderma)

University of Pretoria

1. [No author]. Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa .

Degree: 2012, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-05242012-171645/

► Successful treatment of canine pyoderma has become increasingly difficult due to the development of antimicrobial resistance and recurrence of infection. The development and spread of… (more)

▼ Successful treatment of canine pyoderma has become increasingly difficult due to the development of antimicrobial resistance and recurrence of infection. The development and spread of antimicrobial resistance has major implications because treatment failures have been associated with increased mortality, morbidity and costs related to disease. All canine skin samples submitted to Vetdiagnostix Veterinary Pathology Services for microbiological culture and sensitivity between January 2007 and June 2010, from which Staphylococcus intermedius was isolated, were selected for this investigation. A total of 319 samples from dogs of various ages, sexes and breeds from various locations throughout South Africa were obtained. In addition to the antimicrobial susceptibility data, data relating to dog signalment (age, gender, breed), case history and any other aspects of the history provided e.g. diagnosis, sample type and geographical location of the patient were collected. The number of skin samples yielding Staphylococcus intermedius was high in dogs up to the age of 6 years and, then decreased with only a few cases in dogs aged 11 years or older. The distribution of samples collected in 2010 was unusual in that there were two peaks, one in dogs two years old or less and one in 6 to 9-year-old dogs. With the exception of skin samples taken in 2008, a high percentage of dogs were under the age of one year old. 2008 had a lower percentage of affected dogs less than one year of age compared to the other years. Staphylococcal pyoderma is more common in dogs below the age of five years. Dogs above this age are less likely to contract this condition. The genders tended to be equally distributed throughout the years, with almost equal proportions of affected males and females present. The Bull Terrier types and Shepherd types were grouped separately as they were over-represented and are known to be prone to pyoderma. Large short haired dogs were consistently worse affected throughout the years sampled, followed by the Bull Terrier types. In 2010, small short haired breeds were worse affected compared to the numbers between 2007 and 2009. The monthly distribution, with the exception of 2009, tended to be consistent throughout the years. The unusual temporal distribution in 2009 could be associated with a general distribution in the number of samples compared to the other years sampled. Samples tended to be mainly from practices located in the KwaZulu-Natal province of South Africa. This is most likely due to the fact that the laboratory is located in this province, with a courier network that arranges collection from these practices. Practices in other regions send their samples to the laboratory via private courier companies and the South African Post Office. Antimicrobial resistance of S. intermedius was greatest to ampicillin followed by tetracycline and then potentiated sulphonamides. The results also showed that, in general, antimicrobial resistance was low. Very few methicillin resistant isolates were detected. Temporal trends… Advisors/Committee Members: Prof M van Vuuren (advisor).

Subjects/Keywords: Staphylococcus intermedius; Canine pyoderma; South Africa; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2012). Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05242012-171645/

Chicago Manual of Style (16^th Edition):

author], [No. “Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa .” 2012. Masters Thesis, University of Pretoria. Accessed January 18, 2021. http://upetd.up.ac.za/thesis/available/etd-05242012-171645/.

MLA Handbook (7^th Edition):

author], [No. “Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa .” 2012. Web. 18 Jan 2021.

Vancouver:

author] [. Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa . [Internet] [Masters thesis]. University of Pretoria; 2012. [cited 2021 Jan 18]. Available from: http://upetd.up.ac.za/thesis/available/etd-05242012-171645/.

Council of Science Editors:

author] [. Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa . [Masters Thesis]. University of Pretoria; 2012. Available from: http://upetd.up.ac.za/thesis/available/etd-05242012-171645/

University of Pretoria

2. Blunt, Catherine Ann. Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa.

Degree: Veterinary Tropical Diseases, 2012, University of Pretoria

URL: http://hdl.handle.net/2263/24953

► Successful treatment of canine pyoderma has become increasingly difficult due to the development of antimicrobial resistance and recurrence of infection. The development and spread of… (more)

▼ Successful treatment of canine pyoderma has become increasingly difficult due to the development of antimicrobial resistance and recurrence of infection. The development and spread of antimicrobial resistance has major implications because treatment failures have been associated with increased mortality, morbidity and costs related to disease. All canine skin samples submitted to Vetdiagnostix Veterinary Pathology Services for microbiological culture and sensitivity between January 2007 and June 2010, from which Staphylococcus intermedius was isolated, were selected for this investigation. A total of 319 samples from dogs of various ages, sexes and breeds from various locations throughout South Africa were obtained. In addition to the antimicrobial susceptibility data, data relating to dog signalment (age, gender, breed), case history and any other aspects of the history provided e.g. diagnosis, sample type and geographical location of the patient were collected. The number of skin samples yielding Staphylococcus intermedius was high in dogs up to the age of 6 years and, then decreased with only a few cases in dogs aged 11 years or older. The distribution of samples collected in 2010 was unusual in that there were two peaks, one in dogs two years old or less and one in 6 to 9-year-old dogs. With the exception of skin samples taken in 2008, a high percentage of dogs were under the age of one year old. 2008 had a lower percentage of affected dogs less than one year of age compared to the other years. Staphylococcal pyoderma is more common in dogs below the age of five years. Dogs above this age are less likely to contract this condition. The genders tended to be equally distributed throughout the years, with almost equal proportions of affected males and females present. The Bull Terrier types and Shepherd types were grouped separately as they were over-represented and are known to be prone to pyoderma. Large short haired dogs were consistently worse affected throughout the years sampled, followed by the Bull Terrier types. In 2010, small short haired breeds were worse affected compared to the numbers between 2007 and 2009. The monthly distribution, with the exception of 2009, tended to be consistent throughout the years. The unusual temporal distribution in 2009 could be associated with a general distribution in the number of samples compared to the other years sampled. Samples tended to be mainly from practices located in the KwaZulu-Natal province of South Africa. This is most likely due to the fact that the laboratory is located in this province, with a courier network that arranges collection from these practices. Practices in other regions send their samples to the laboratory via private courier companies and the South African Post Office. Antimicrobial resistance of S. intermedius was greatest to ampicillin followed by tetracycline and then potentiated sulphonamides. The results also showed that, in general, antimicrobial resistance was low. Very few methicillin resistant isolates were detected. Temporal trends… Advisors/Committee Members: Van Vuuren, Moritz (advisor).

Subjects/Keywords: Staphylococcus intermedius; Canine pyoderma; South Africa; UCTD; Dogs – Diseases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Blunt, C. A. (2012). Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24953

Chicago Manual of Style (16^th Edition):

Blunt, Catherine Ann. “Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa.” 2012. Masters Thesis, University of Pretoria. Accessed January 18, 2021. http://hdl.handle.net/2263/24953.

MLA Handbook (7^th Edition):

Blunt, Catherine Ann. “Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa.” 2012. Web. 18 Jan 2021.

Vancouver:

Blunt CA. Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa. [Internet] [Masters thesis]. University of Pretoria; 2012. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/2263/24953.

Council of Science Editors:

Blunt CA. Antimicrobial susceptibility profiles of Staphylococcus intermedius isolates from clinical cases of canine pyoderma in South Africa. [Masters Thesis]. University of Pretoria; 2012. Available from: http://hdl.handle.net/2263/24953

University of Edinburgh

3. Bannoehr, Jeanette. Molecular population and colonisation factor analysis of the Staphylococcus intermedius group.

Degree: PhD, 2010, University of Edinburgh

URL: http://hdl.handle.net/1842/5716

► The Gram-positive bacterium Staphylococcus intermedius is regarded as the major cause of canine pyoderma, a common skin infection of dogs. However, despite its clinical importance,… (more)

▼ The Gram-positive bacterium Staphylococcus intermedius is regarded as the major cause of canine pyoderma, a common skin infection of dogs. However, despite its clinical importance, the population genetic structure of S. intermedius is poorly understood. The current study examined the population genetic structure of S. intermedius using a multilocus DNA sequencing approach. A collection of 99 isolates phenotypically identified as S. intermedius and originating from a broad array of animal hosts in several different countries was investigated. Phylogenetic analysis indicated that the isolates belonged to three distinct species including S. intermedius, staphylococcus pseudintermedius, and Staphylococcus delphini, together referred to as the S. intermedius group (SIG). Importantly, it was discovered that all canine isolates investigated belonged to the S. pseudintermedius phylotype and it was concluded that S. pseudintermedius, not S. intermedius, is the common cause of canine pyoderma. Further, it was revealed that S. delphini is more clinically important than was previously thought. The allelic variation of agrD, which encodes the autoinducing peptide (AIP) of the agr quorum sensing system in staphylococci, was determined for all isolates. Four AIP variants were identified, including three which were present in all three species, suggesting that a common quorum sensing capacity has been conserved despite species differentiation in very different niches. Considerable clonal diversity was revealed within the S. pseudintermedius species, including several methicillin-resistant clones which have evolved by recent acquisition of the mecA gene. Using the sequence diversity identified, a simple diagnostic test was developed based on a PCR-RFLP approach to discriminate S. pseudintermedius from S. intermedius and S. delphini. Having established that S. pseudintermedius is the common canine pyoderma pathogen, this study aimed to investugate key host-pathogen interactions involved in colonisation of its canine host. Bioinformatic analysis of the whole genome sequence of a clinical isolate of S. pseudintermedius (strain ED99) revealed 17 genes encoding predicted LPXTG-containing cell wall-anchored (CWA) surface proteins. A diverse collection of S. pseudintermedius isolates and closely related staphylococcal species was screened for the presence of the genes encodng the novel CWA proteins. The majority of genes were widely distributed among the isolates examined, with nine genes being exclusive to S. pseudintermedius and eight being also present in other members of the SIG. In Gram-positive bacteria, a family of CWA proteins called microbial surgace components recognising adhesive matrix molecules (MSCRAMMs)mediates bacterial adherence to extracellular matrix proteins of the host. Three of the 17 predicted novel CWA proteins, designated SpsD, SpsL and SpsO, were selected for further characterisation of their role in host-pathogen interactions and were cloned and expressed on the surface of the surrogate host Lactococcus lactis.…

Subjects/Keywords: 636.089; staphylococcus pseudintermedius; canine pyoderma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bannoehr, J. (2010). Molecular population and colonisation factor analysis of the Staphylococcus intermedius group. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5716

Chicago Manual of Style (16^th Edition):

Bannoehr, Jeanette. “Molecular population and colonisation factor analysis of the Staphylococcus intermedius group.” 2010. Doctoral Dissertation, University of Edinburgh. Accessed January 18, 2021. http://hdl.handle.net/1842/5716.

MLA Handbook (7^th Edition):

Bannoehr, Jeanette. “Molecular population and colonisation factor analysis of the Staphylococcus intermedius group.” 2010. Web. 18 Jan 2021.

Vancouver:

Bannoehr J. Molecular population and colonisation factor analysis of the Staphylococcus intermedius group. [Internet] [Doctoral dissertation]. University of Edinburgh; 2010. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1842/5716.

Council of Science Editors:

Bannoehr J. Molecular population and colonisation factor analysis of the Staphylococcus intermedius group. [Doctoral Dissertation]. University of Edinburgh; 2010. Available from: http://hdl.handle.net/1842/5716

Technical University of Lisbon

4. Mascarenhas, Diana Marisa Cardoso. Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar.

Degree: 2014, Technical University of Lisbon

URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236

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Dissertação de Mestrado Integrado em Medicina Veterinária

Nos cães com dermatite atópica, as infeções cutâneas recorrentes causadas pelo Staphylococcus pseudintermedius são extremamente comuns. Estudos prévios… (more)

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Dissertação de Mestrado Integrado em Medicina Veterinária

Nos cães com dermatite atópica, as infeções cutâneas recorrentes causadas pelo Staphylococcus pseudintermedius são extremamente comuns. Estudos prévios demonstraram que cães atópicos com piodermite recorrente têm títulos mais elevados de imunoglobulina E anti-Staphylococcus no soro. Os principais objetivos deste estudo foram definir qual a concentração ideal de extrato de alergénios de S. pseudintermedius a utilizar nos testes intradérmicos de cães atópicos e posteriormente determinar a frequência de hipersensibilidade cutânea ao S. pseudintermedius em cães atópicos com história de infeção cutânea recorrente. Para isso, realizaram-se testes intradérmicos, onde se testaram diferentes concentrações de extrato de um S. pseudintermedius resistente à meticilina (MRSP), estirpe 5819/10. Esta estirpe foi isolada de um cão com piodermite profunda e apresentava os seguintes genes de virulência: luk-I, se-int, siet, speta, ebpS e spsL. As concentrações utilizadas foram 2 μg/ml, 20 μg/ml e 200 μg/ml, assim como um controlo positivo (fosfato de histamina 0.001%) e um controlo negativo (PBS 0.9%). Foram incluídos neste estudo, vinte e três cães saudáveis e vinte e quatro cães atópicos com infeção cutânea recorrente por S. pseudintermedius, diagnosticados por citologia cutânea e/ou cultura bacteriana. Dos 23 cães saudáveis presentes neste estudo, 14 (61%) apresentaram reação positiva na concentração de 200 μg/ml. Assim sendo, é bastante provável que a concentração de 200 μg/ml seja irritante e por isso resulte em reações falso-positivas. A concentração de 20 μg/ml foi a concentração mais indicada para ser usada no diagnóstico de hipersensibilidade ao S. pseudintermedius em cães atópicos. Posteriormente, foram realizados testes intradérmicos em 24 canídeos atópicos com piodermite e/ou otite bacteriana recorrente com a concentração de 20 μg/ml; quatro destes animais apresentaram reações positivas nesta concentração, o que parecer indicar a existência de reações de hipersensibilidade imediata a componentes do S. pseudintermedius. No entanto, limitações que envolviam o extrato impediram a obtenção de resultados reprodutíveis e uniformes. Acreditamos que a imunoterapia específica para esta bactéria pode ser benéfica para o tratamento destes pacientes, o que torna fundamental o diagnóstico de hipersensibilidade ao S. pseudintermedius. Nesse sentido, deverão ser realizados mais estudos, de modo a obter um extrato fidedigno que permite alcançar consistência e reprodutibilidade em ambiente clínico e assim melhorar a qualidade dos resultados obtidos nos testes intradérmicos.

ABSTRACT - THE USE OF STAPHYLOCOCCUS PSEUDINTERMEDIUS EXTRACT IN SKIN ALLERGY TESTS ON THE DOG – A PRELIMINARY STUDY - In dogs with atopic dermatitis, recurrent skin infections caused by Staphylococcus pseudintermedius are extremely common. Previous studies demonstrated that atopic dogs with recurrent pyoderma have higher titters of anti-Staphylococcus immunoglobulin E. The main objectives of this study…

Advisors/Committee Members: Lourenço, Ana Mafalda Gonçalves Xavier Félix, Pomba, Maria Constança Matias Ferreira.

Subjects/Keywords: S. pseudintermedius; Extrato de alergénios; Infeção cutânea recorrente; Dermatite atópica canina; Allergens extracts; Recurrent pyoderma; Canine atopic dermatitis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mascarenhas, D. M. C. (2014). Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mascarenhas, Diana Marisa Cardoso. “Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar.” 2014. Thesis, Technical University of Lisbon. Accessed January 18, 2021. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mascarenhas, Diana Marisa Cardoso. “Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar.” 2014. Web. 18 Jan 2021.

Vancouver:

Mascarenhas DMC. Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar. [Internet] [Thesis]. Technical University of Lisbon; 2014. [cited 2021 Jan 18]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mascarenhas DMC. Utilização de extracto de Staphylococcus pseudintermedius em testes alergológicos cutâneos no cão : um estudo preliminar. [Thesis]. Technical University of Lisbon; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/7236

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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