subject:(Candida albicans)

1. Scaduto, Christine Maria. Mechanisms of Sexual Reproduction in Human Fungal Pathogens.

Degree: Department of Molecular Biology, Cell Biology and Biochemistry, 2018, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:792587/

► Sexual reproduction typically involves fusion of haploid gametes to form diploid cells, which subsequently undergo meiosis to regenerate the haploid form. Despite the ubiquity of… (more)

Subjects/Keywords: Candida albicans

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APA (6^th Edition):

Scaduto, C. M. (2018). Mechanisms of Sexual Reproduction in Human Fungal Pathogens. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792587/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Scaduto, Christine Maria. “Mechanisms of Sexual Reproduction in Human Fungal Pathogens.” 2018. Thesis, Brown University. Accessed February 25, 2020. https://repository.library.brown.edu/studio/item/bdr:792587/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Scaduto, Christine Maria. “Mechanisms of Sexual Reproduction in Human Fungal Pathogens.” 2018. Web. 25 Feb 2020.

Vancouver:

Scaduto CM. Mechanisms of Sexual Reproduction in Human Fungal Pathogens. [Internet] [Thesis]. Brown University; 2018. [cited 2020 Feb 25]. Available from: https://repository.library.brown.edu/studio/item/bdr:792587/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Scaduto CM. Mechanisms of Sexual Reproduction in Human Fungal Pathogens. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792587/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Vieira, Ana Paula Coelho. Eficácia de limpadores químicos na remoção e re-colonização de biofilmes de Candida spp. formados na superfície de material reembasador .

Degree: 2010, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288379

► Resumo: Os reembasadores para base de prótese, após a exposição à cavidade bucal, apresentam alterações de superfície facilitando a adesão e a colonização por micro-organismos.… (more)

▼ Resumo: Os reembasadores para base de prótese, após a exposição à cavidade bucal, apresentam alterações de superfície facilitando a adesão e a colonização por micro-organismos. Para a limpeza de superfície desses materiais são indicados os limpadores químicos para evitar os danos mecânicos que podem ser provocados pelas cerdas das escovas dentais. Assim, o objetivo nesta pesquisa foi avaliar a eficácia de limpadores químicos na remoção de biofilme de Candida spp. desenvolvido sobre a superfície de um reembasador classificado como permanente à base de poli-metilmetacrilato e na prevenção da re-colonização dessa superfície, especialmente a Candida spp., comumente associada ao desenvolvimento da candidíase. Espécimes (10 mm diâmetro X 3 mm altura) de resina acrílica reembasada com um reembasador mais representativo disponível comercialmente teve sua rugosidade de superfície mensurada antes (baseline) e biofilme de C. albicans ATCC 90028 ou C. glabrata ATCC 2001 foi desenvolvido sobre os mesmos. Após a formação dos biofilmes os espécimes foram aleatorizados e submetidos aos tratamentos (n=16): AD - Água destilada (Controle), 15 min; POL - Polident 3 minutos, 3 min; EFF - Efferdent, 15 min; HPS - Hipoclorito de sódio a 0,5%, 10 minutos. Metade destes espécimes (n=8) foi utilizada para determinação da eficácia dos limpadores, utilizando contagem de células viáveis, enquanto os espécimes remanescentes (n=8), após os tratamentos, foram novamente colocados em meio de cultura estéril e incubados por mais 48 h a fim de determinar o efeito dos limpadores na prevenção da recolonização. Após os tratamentos os espécimes tiveram a rugosidade de superfície determinada, considerada pós-tratamento. Alguns espécimes de cada uma das espécies de Candida tiveram a superfície analisada após os tratamentos, por microscopia eletrônica de varredura (MEV). Os dados foram submetidos à análise de variância e teste de Tukey HSD em nível de significância de 5%. A rugosidade de superfície foi significantemente maior após os tratamentos (P<0,05). Quantos aos tratamentos, o HPS mostrou-se efetivo tanto para a desinfecção quanto na recolonização de ambas as espécies de Candida, pois houve ausência total de crescimento. Na avaliação da desinfecção, imediatamente após os tratamentos, quando C. albicans foi considerada, não houve diferença significativa entre os peróxidos alcalinos (p>0,05) e ambos diminuíram o número de células fúngicas (p<0,05) comparado ao tratamento com AD. Entretanto, para C. glabrata, os tratamentos com ADD e peróxidos alcalinos não se diferenciaram entre si (p>0,05). Na análise dos resultados para a recolonização foi observada que houve inversão no comportamento, pois enquanto, para C. albicans, os tratamentos com AD e peróxidos alcalinos não diferiram entre si (p>0,05), para C. glabrata os tratamentos com peróxidos alcalinos apresentaram valores similares e menores (p>0,05),quando comparados com o tratamento com AD (p<0,05). Na comparação entre as espécies de Candida observou-se que C. glabrata apresentou os maiores níveis de… Advisors/Committee Members: Del Bel Cury, Altair Antoninha, 1948- (advisor), Silva, Wander José da, 1980- (advisor), Rocha, Eduardo Passos (committee member), Cenci, Tatiana Pereira (committee member).

Subjects/Keywords: Candida albicans; Candida glabrata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vieira, A. P. C. (2010). Eficácia de limpadores químicos na remoção e re-colonização de biofilmes de Candida spp. formados na superfície de material reembasador . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288379

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vieira, Ana Paula Coelho. “Eficácia de limpadores químicos na remoção e re-colonização de biofilmes de Candida spp. formados na superfície de material reembasador .” 2010. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288379.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vieira, Ana Paula Coelho. “Eficácia de limpadores químicos na remoção e re-colonização de biofilmes de Candida spp. formados na superfície de material reembasador .” 2010. Web. 25 Feb 2020.

Vancouver:

Vieira APC. Eficácia de limpadores químicos na remoção e re-colonização de biofilmes de Candida spp. formados na superfície de material reembasador . [Internet] [Thesis]. Universidade Estadual de Campinas; 2010. [cited 2020 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288379.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vieira APC. Eficácia de limpadores químicos na remoção e re-colonização de biofilmes de Candida spp. formados na superfície de material reembasador . [Thesis]. Universidade Estadual de Campinas; 2010. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288379

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

3. Gomes, Priscila Nogueira. Bioatividade, bioestrutura e morfologia de biofilmes de Candida spp. desenvolvidos na presença de fluconazol .

Degree: 2011, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288079

► Resumo: O biofilme de Candida spp. formado na superfície de próteses removíveis, é o principal fator etiológico da candidose, sendo a C. albicans e a… (more)

▼ Resumo: O biofilme de Candida spp. formado na superfície de próteses removíveis, é o principal fator etiológico da candidose, sendo a C. albicans e a C. glabrata as espécies mais prevalentes nesta condição. O antifúngico fluconazol (FLZ) é frequentemente utilizado no tratamento da candidose, porém o sucesso tem sido limitado devido a resistência desenvolvida pela Candida a esse medicamento. Considerando a importância da estrutura e morfologia do biofilme de Candida na candidose, o objetivo neste estudo foi avaliar o efeito de FLZ na bioatividade, bioestrutura e morfologia celular de biofilmes de Candida spp. desenvolvidos na presença deste antifúngico. Espécimes (10 mm x 2 mm) foram confeccionados utilizando resina de poli(metilmetacrilato) (PMMA), polimerizada por banho de água quente. Películas de saliva foram formadas na superfície da PMMA, e biofilmes de um isolado referência e dois isolados clínicos de C. albicans (ATCC 90028, P01, P34) e C. glabrata (ATCC 2001, P11, P31) foram desenvolvidos por 48h. Dois grupos foram formados: controle e experimental. FLZ a 2,56 µg/mL, concentração biodisponivel na saliva, foi adicionado ao meio de cultura do grupo experimental. Os meios de cultura do grupo controle e experimental foram trocados a cada 24 h. As bioatividades dos biofilmes foram avaliadas utilizando análise colorimétrica de redução por XTT. A bioestrutura analisada através do Microscópio Confocal à Laser e a morfologia celular avaliada utilizando o Microscópio Eletrônico de Transmissão. Os dados foram analisados pelo Test t de Student com nível de significância de 5%. A presença do FLZ reduziu a bioatividade de todos os biofilmes de C. albicans (p<0.001), porém não alterou a estrutura e morfologia da C. albicans P34. Quanto à bioatividade e bioestrutura dos biofilmes de C. glabrata, não foram encontradas diferenças estatisticamente significantes entre os grupos controle e experimental. Pode-se concluir que as alterações da bioatividade, bioestrutura e morfologia celular, como resposta ao tratamento com FLZ, na concentração biodisponível na saliva, depende da cepa de Candida spp. avaliada.; Abstract: Candida spp. biofilm formed on removable denture surfaces is considered the main etiologic factor of candidosis, being the C. albicans and C.glabrata the species most frequently found in this condition. The antifungic fluconazol (FLZ) is commonly used in the treatment of candidosis, however its success is limited due to the resistance developed by Candida to this medicament. Considering the importance of the structure and morphology of Candida biofilms in the candidosis, the aim of this study was to evaluate the effect of FLZ on the bioactivity, biostructure and morphology of Candida spp. biofilms formed in the presence of this antifungal agent. Specimens (10 mm x 2 mm) were fabricated using water bath poly(methylmethacrylate) resin (PMMA). Salivary pellicles were formed on the PMMA surface, and biofilms of a reference strain and two clinical isolates of C. albicans (ATCC 90028, P01, P34) and C. glabrata (ATCC 2001,… Advisors/Committee Members: Del Bel Cury, Altair Antoninha, 1948- (advisor), Moyses, Marcos Ribeiro (committee member), Ribeiro, João Gustavo Rabelo (committee member), Cenci, Tatiana Pereira (committee member), Garcia, Renata Cunha Matheus Rodrigues (committee member).

Subjects/Keywords: Candida albicans; Candida glabrata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gomes, P. N. (2011). Bioatividade, bioestrutura e morfologia de biofilmes de Candida spp. desenvolvidos na presença de fluconazol . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gomes, Priscila Nogueira. “Bioatividade, bioestrutura e morfologia de biofilmes de Candida spp. desenvolvidos na presença de fluconazol .” 2011. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gomes, Priscila Nogueira. “Bioatividade, bioestrutura e morfologia de biofilmes de Candida spp. desenvolvidos na presença de fluconazol .” 2011. Web. 25 Feb 2020.

Vancouver:

Gomes PN. Bioatividade, bioestrutura e morfologia de biofilmes de Candida spp. desenvolvidos na presença de fluconazol . [Internet] [Thesis]. Universidade Estadual de Campinas; 2011. [cited 2020 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gomes PN. Bioatividade, bioestrutura e morfologia de biofilmes de Candida spp. desenvolvidos na presença de fluconazol . [Thesis]. Universidade Estadual de Campinas; 2011. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

4. Zhang, Ningxin. Sex has no detectable net benefits for Candida albicans.

Degree: PhD, Molecular Microbiology, 2012, Massey University

URL: http://hdl.handle.net/10179/3679

► Like many other important opportunistic human fungal pathogens, for more than a century Candida albicans was thought to be strictly asexual until a parasexual cycle… (more)

▼ Like many other important opportunistic human fungal pathogens, for more than a century Candida albicans was thought to be strictly asexual until a parasexual cycle was recently discovered in the laboratory. It is uncertain, however, whether sex is still a viable reproductive strategy for C. albicans. In this study I tested whether or not mating enhanced survival of parental genes in this yeast, by mating 10 clinical isolates and testing recombinants’ fitness. Clinical isolates of C.albicans usually are diploid, carrying both the MTLa and MTLα mating type alleles, each on a different copy of chromosome 5. These strains are apparently incapable of meiosis and cannot mate with each other, because the a1-α2 heterodimer suppresses mating. Through selection on sorbose-containing agar I induced loss of MTL-heterozygosity and generated 5 MTLa and 5 MTLα derivatives of clinical isolates. Existing mating techniques involve the use of auxotrophic markers, requiring time-consuming sequential disruption of two copies of biosynthetic genes if wild-type clinical isolates are to be mated. Furthermore, auxotrophy affects the virulence of a strain, and this can potentially interfere with comparing the fitness of recombinants with that of their parents. I therefore developed a method for mating clinical isolates marked with two drug resistance markers, the mycophenolic acid (MPA) resistance-conferring allele of IMH3 and the nourseothricin (NAT) resistance gene CaNAT1, allowing selection of recombinants on the basis of resistance to both agents. I marked all MTLa strains with the MPA resistance gene and all MTLα strains with the NAT resistance gene. This allowed 25 combinations for mating. Recombinants were obtained from 15 combinations of 9 strains. It was found that not all C. albicans clinical isolates could mate. Using growth rate as the criterion, I tested the fitness of clinical isolates, MTLhomozygous derivatives with and without resistance markers and recombinants during adaptation to a novel environment (YPD medium), maximizing the potential benefits of sex. After computationally correcting for the impact of experimental manipulations, I calculated the net benefit of sex as the difference in the number of offspring from two cells that become mating competent and engage in sex compared to the offspring they could have produced by continued clonal reproduction. My results indicated that, as a rule, engaging in sex reduces the chances of survival of C. albicans’ genes, in part because MTL homozygosis significantly reduced growth rates. Through fitness increase after recombination, sex may eventually confer a net benefit for some strain combinations in the laboratory, but this probably occurs too late to prevent elimination of recombinants by competition and genetic drift in nature. Sex in C. albicans therefore diminished parents’ chances to pass on their genes to future generations. These findings have a significant impact on the assessment of the role of sex in C. albicans and other …

Subjects/Keywords: Pathogenic fungi; Candida albicans; Candida albicans reproduction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, N. (2012). Sex has no detectable net benefits for Candida albicans. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/3679

Chicago Manual of Style (16^th Edition):

Zhang, Ningxin. “Sex has no detectable net benefits for Candida albicans.” 2012. Doctoral Dissertation, Massey University. Accessed February 25, 2020. http://hdl.handle.net/10179/3679.

MLA Handbook (7^th Edition):

Zhang, Ningxin. “Sex has no detectable net benefits for Candida albicans.” 2012. Web. 25 Feb 2020.

Vancouver:

Zhang N. Sex has no detectable net benefits for Candida albicans. [Internet] [Doctoral dissertation]. Massey University; 2012. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/10179/3679.

Council of Science Editors:

Zhang N. Sex has no detectable net benefits for Candida albicans. [Doctoral Dissertation]. Massey University; 2012. Available from: http://hdl.handle.net/10179/3679

Stellenbosch University

5. Stone, Wendy. An external ecological niche for Candida albicans within reducing, oxygen-limited zones of wetlands and riverbanks.

Degree: MSc, Microbiology, 2011, Stellenbosch University

URL: http://hdl.handle.net/10019.1/6892

► ENGLISH ABSTRACT: The ascomycetous yeast, Candida albicans, has been almost exclusively studied in a clinical context, due to the medical risk and costs associated with… (more)

▼ ENGLISH ABSTRACT: The ascomycetous yeast, Candida albicans, has been almost exclusively studied in a clinical context, due to the medical risk and costs associated with the yeast. Most environmental research into the external survival of this opportunistic pathogen has been concerned with short-term, severe pollution challenges. However, a study of literature indicates that the habitat characteristics of the oxygenlimited zones in wetlands and riverbanks are comparable to those of the gastrointestinal source of sewage-borne C. albicans. Interestingly, these are the external, environmental regions to which sewage-borne C. albicans is often exposed. In addition, oxygen-limitation is the predominant parameter in stimulating conjugation of C. albicans. Based on these observations, this study aimed to assess polluted river bank and wetland environments in the Western Cape of South Africa as potential habitats to accommodate a niche for C. albicans, particularly comparing the presence of this yeast in oxygen-limited, plant debris-rich zones and aerobic, clear, flowing zones. The second objective was to employ in vitro microcosm studies to investigate the survival and growth of C. albicans in various microhabitats similar to those comprising the oxygen-limited zones of wetlands. These included the rhizosphere of wetland flora, various soil and mud types and decomposing plant debris. The final objective was to establish the presence of sufficient nutrient and energy sources within this environment for the growth of C. albicans. In particular, cellulosic substrates and mono- and disaccharides released by the natural degradation of wetland plant debris were investigated as potential energy sources for this human commensal in the wetland environment. These study objectives combined to demonstrate the potential of such an oxygen-limited, plant debris-rich environment as a niche for C. albicans external to its human host. Both semi-quantitative culturing techniques and quantitative Real-Time PCR demonstrated the improved survival of C. albicans in oxygen-limited, plant debris-rich zones in wetland and river bank environments, in comparison to aerobic, clear subsurface water zones in the same environments. These zones were compared in the Plankenburg and Diep Rivers, situated in the Western Cape of South Africa. Correlations between coliform concentrations and total yeast concentrations were demonstrated in each of the different river zones, with higher pollution levels characteristic of the dry season. Candida albicans numbers in flowing water (zone W), rock-filtered (zone R) and plant-filtered water (zone P) were compared during the progress of the rainy and dry seasons. No C. albicans was observed in clear, flowing water throughout the analysis. Early in the rainy season, both rock-filtered (aerobic, poor in plant debris) and plant-filtered (oxygen-limited, rich in plant debris) water demonstrated C. albicans numbers at approximately equivalent levels of 10²-10³ cells/100 mL. However, as the rainy … Advisors/Committee Members: Botha, Alf, Wilsenach, Jac, Stellenbosch University. Faculty of Science. Dept. of Microbiology..

Subjects/Keywords: Candida albicans; Candida albicans – Survival; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stone, W. (2011). An external ecological niche for Candida albicans within reducing, oxygen-limited zones of wetlands and riverbanks. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/6892

Chicago Manual of Style (16^th Edition):

Stone, Wendy. “An external ecological niche for Candida albicans within reducing, oxygen-limited zones of wetlands and riverbanks.” 2011. Masters Thesis, Stellenbosch University. Accessed February 25, 2020. http://hdl.handle.net/10019.1/6892.

MLA Handbook (7^th Edition):

Stone, Wendy. “An external ecological niche for Candida albicans within reducing, oxygen-limited zones of wetlands and riverbanks.” 2011. Web. 25 Feb 2020.

Vancouver:

Stone W. An external ecological niche for Candida albicans within reducing, oxygen-limited zones of wetlands and riverbanks. [Internet] [Masters thesis]. Stellenbosch University; 2011. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/10019.1/6892.

Council of Science Editors:

Stone W. An external ecological niche for Candida albicans within reducing, oxygen-limited zones of wetlands and riverbanks. [Masters Thesis]. Stellenbosch University; 2011. Available from: http://hdl.handle.net/10019.1/6892

University of Aberdeen

6. Tilliman, Anna Theresa. Nitrosative and combinatorial stress responses in Candida albicans.

Degree: PhD, 2013, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192303 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569642

► Fungal nitrosative stress responses are understudied and poorly understood. Also, while the responses of the pathogen Candida albicans to individual oxidative and cationic stresses have… (more)

▼ Fungal nitrosative stress responses are understudied and poorly understood. Also, while the responses of the pathogen Candida albicans to individual oxidative and cationic stresses have been characterised relatively well, the responses of this pathogen to simultaneous combinations of these and nitrosative stresses have not been elaborated. Yet, C. albicans is exposed to these combinatorial stresses during disease progression. Therefore, the overall aim of this project was to characterise the "Nitrosative and Combinatorial Stress Responses in Candida albicans". The first objective was to elucidate nitrosative stress response mechanisms in C. albicans at a cellular, transcriptional and post-translational level. Genetic screens revealed that the Hog1 and Mkc1 signalling pathways contribute to nitrosative stress adaptation in addition to Cta4 signalling. The next objective was to characterise responses to combinatorial nitrosative, oxidative and/or cationic stresses with respect to the relevant signal transduction pathways. Unexpectedly, combinatorial stresses did not activate classical nitrosative (Cta4) and oxidative (Cap1) signalling mechanisms. Instead, Hog1 signalling was found to play a significant role in the combinatorial stress adaptation. Dramatic changes in the GSH redox potential were observed in response to both single and combinatorial stresses. Thus, GSH redox regulatory mechanisms were explored in more detail. BLAST searches of the C. albicans genome were performed, revealing genes encoding a putative NADPH-dependent glutathione reductase (Glr1) and a GSH-dependent S-nitrosoglutathione reductase (Fdh3). We confirmed that both conserved enzymes are involved in the maintenance of redox homeostasis and hence are crucial for C. albicans adaptation during host-pathogen interactions. This was achieved by showing that inactivation of Glr1 or Fdh3 disturbs GSH redox homeostasis, confers oxidative and formaldehyde stress sensitivity and attenuates virulence.

Subjects/Keywords: 610; Candida Albicans

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APA (6^th Edition):

Tilliman, A. T. (2013). Nitrosative and combinatorial stress responses in Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192303 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569642

Chicago Manual of Style (16^th Edition):

Tilliman, Anna Theresa. “Nitrosative and combinatorial stress responses in Candida albicans.” 2013. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192303 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569642.

MLA Handbook (7^th Edition):

Tilliman, Anna Theresa. “Nitrosative and combinatorial stress responses in Candida albicans.” 2013. Web. 25 Feb 2020.

Vancouver:

Tilliman AT. Nitrosative and combinatorial stress responses in Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2013. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192303 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569642.

Council of Science Editors:

Tilliman AT. Nitrosative and combinatorial stress responses in Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2013. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192303 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569642

University of Aberdeen

7. Ene, Iuliana V. Impact of host carbon sources in the pathobiology of Candida albicans.

Degree: PhD, 2012, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=189402 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558630

► Candida albicans is the most common systemic fungal pathogen of humans. Virulence factors, fitness attributes and the host immunity ultimately determine the ability of C.… (more)

Subjects/Keywords: 610; Candida Albicans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ene, I. V. (2012). Impact of host carbon sources in the pathobiology of Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=189402 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558630

Chicago Manual of Style (16^th Edition):

Ene, Iuliana V. “Impact of host carbon sources in the pathobiology of Candida albicans.” 2012. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=189402 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558630.

MLA Handbook (7^th Edition):

Ene, Iuliana V. “Impact of host carbon sources in the pathobiology of Candida albicans.” 2012. Web. 25 Feb 2020.

Vancouver:

Ene IV. Impact of host carbon sources in the pathobiology of Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2012. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=189402 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558630.

Council of Science Editors:

Ene IV. Impact of host carbon sources in the pathobiology of Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2012. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=189402 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558630

University of Aberdeen

8. Leach, Michelle D. Impact of post-translational modifications during stress adaptation in Candida albicans.

Degree: 2011, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167804 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553804

► Organisms exist in complex and dynamic environments. Facing numerous challenges, microorganisms must continuously monitor environmental changes and adapt to these if they are to survive.… (more)

▼ Organisms exist in complex and dynamic environments. Facing numerous challenges, microorganisms must continuously monitor environmental changes and adapt to these if they are to survive. For example, the major fungal pathogen of humans, Candida albicans experiences diverse stresses, including temperature fluctuations, oxidative stress and enzymic processes, that cause molecular damage. Post-translational modifications such as phosphorylation play major roles in stress adaptation, for example through activation of MAP kinase pathways and transcription factors such as the heat shock transcription factor, Hsf1. However, other post-translational modifications such as ubiquitination and sumoylation have been relatively understudied. Nevertheless, they are believed to play crucial regulatory roles in many cellular processes including stress adaptation. Therefore, in this study the roles of ubiquitin and SUMO (small ubiquitin-like modifier) have been investigated in C. albicans. Proteomics was used to identify ubiquitination and sumoylation targets, and this was combined with molecular analyses of the UBI4 and SMT3 genes, which encode polyubiquitin and SUMO, respectively. Both ubiquitination and sumoylation were shown to play important roles in morphogenesis, cell division and stress adaptation in C. albicans, including adaptation to heat and oxidative stresses. In addition, the dynamics of heat shock adaptation were examined in C. albicans using a systems biology approach. Hsf1 is known to activate HSP90. In this study, Hsf1 was found to be transiently phosphorylated in response to heat shock, and Hsp90 was found to down-regulate this Hsf1 phosphorylation. This led to the identification of an autoregulatory loop that controls thermal adaptation in C. albicans. A mathematical model of heat shock regulation (constructed in collaboration with Katarzyna Tyc and Edda Klipp) provided novel insights into the regulation of this evolutionarily conserved environmental response and the significance of thermal adaptation during systemic Candida infection.

Subjects/Keywords: 616.969; Candida Albicans

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APA (6^th Edition):

Leach, M. D. (2011). Impact of post-translational modifications during stress adaptation in Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167804 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553804

Chicago Manual of Style (16^th Edition):

Leach, Michelle D. “Impact of post-translational modifications during stress adaptation in Candida albicans.” 2011. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167804 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553804.

MLA Handbook (7^th Edition):

Leach, Michelle D. “Impact of post-translational modifications during stress adaptation in Candida albicans.” 2011. Web. 25 Feb 2020.

Vancouver:

Leach MD. Impact of post-translational modifications during stress adaptation in Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2011. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167804 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553804.

Council of Science Editors:

Leach MD. Impact of post-translational modifications during stress adaptation in Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2011. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167804 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553804

University of Aberdeen

9. Nather, Kerstin. Dynamics and heterogeneity of Candida albicans cell surface proteins.

Degree: 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158587 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531890

► Candida albicans is the major fungal pathogen of humans and its cell surface mannoproteins play important roles in adhesion, interactions with the host and signal… (more)

▼ Candida albicans is the major fungal pathogen of humans and its cell surface mannoproteins play important roles in adhesion, interactions with the host and signal transduction. Most of the covalently attached mannoproteins are linked to the cell surface by glycosylphosphatidylinositol (GPI) anchors. Over 100 putative GPI-proteins have been identified in silico in the C. albicans genome, yet the majority remain uncharacterised with regards to their function and regulation. This study uses different approaches to define the role and regulation of selected GPI-protein genes. Expression analyses demonstrated transcriptional regulation of these genes in response to membrane and wall stresses including antifungal drugs. We propose that these genes encode proteins with important roles in cell wall remodelling. One protein, Pga54 was selected for further analysis. Null mutant and reintegrant strains were constructed and phenotypically analysed. The mutant phenotype was for the greatest parts inconclusive, but it showed a minor virulence defect in a mouse model of systemic infection. Using a tagged version of Pga54 the protein could be detected in the GPI fraction of the cell wall in wild type C. albicans cells and was shown to be higher expressed in cells subjected to stress. To establish whether they play a role in adhesion to host cells selected uncharacterised GPI proteins were heterologously expressed in the non-adherent yeast Saccharomyces cerevisiae. The newly generated S. cerevisiae strains did not show any differences in adhesion to human buccal epithelial cells. Further, the examination of tandem repeat variation in genes encoding GPI anchored proteins in different C. albicans strains indicates that this is could be a further means of creating diversity and heterogeneity at the cell surface. This study employs different methods to study the role and the dynamics of cell surface proteins. In future studies these approaches could be applied to all identified GPI proteins to obtain a comprehensive picture of cell surface diversity in Candida albicans.

Subjects/Keywords: 616.9; Candida Albicans

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APA (6^th Edition):

Nather, K. (2010). Dynamics and heterogeneity of Candida albicans cell surface proteins. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158587 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531890

Chicago Manual of Style (16^th Edition):

Nather, Kerstin. “Dynamics and heterogeneity of Candida albicans cell surface proteins.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158587 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531890.

MLA Handbook (7^th Edition):

Nather, Kerstin. “Dynamics and heterogeneity of Candida albicans cell surface proteins.” 2010. Web. 25 Feb 2020.

Vancouver:

Nather K. Dynamics and heterogeneity of Candida albicans cell surface proteins. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158587 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531890.

Council of Science Editors:

Nather K. Dynamics and heterogeneity of Candida albicans cell surface proteins. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158587 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531890

10. Rojas Zumaeta, Luis Alberto. Especies del genéro Candida implicadas en estomatitis subprotésica de pacientes del Departamento de Odontoestomatología del Centro Médico Naval "CMST"-2007.

Degree: 2008, National University of San Marcos

URL: http://cybertesis.unmsm.edu.pe/handle/cybertesis/2184 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F2184/1/bitstream

►

El propósito del presente fue de identificar las especies de Candida implicadas en estomatitis subprotésica en pacientes del Departamento de Odontoestomatología del Centro Médico Naval… (more)

Subjects/Keywords: Estomatitis; Candida albicans

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APA (6^th Edition):

Rojas Zumaeta, L. A. (2008). Especies del genéro Candida implicadas en estomatitis subprotésica de pacientes del Departamento de Odontoestomatología del Centro Médico Naval "CMST"-2007. (Thesis). National University of San Marcos. Retrieved from http://cybertesis.unmsm.edu.pe/handle/cybertesis/2184 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F2184/1/bitstream

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rojas Zumaeta, Luis Alberto. “Especies del genéro Candida implicadas en estomatitis subprotésica de pacientes del Departamento de Odontoestomatología del Centro Médico Naval "CMST"-2007.” 2008. Thesis, National University of San Marcos. Accessed February 25, 2020. http://cybertesis.unmsm.edu.pe/handle/cybertesis/2184 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F2184/1/bitstream.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rojas Zumaeta, Luis Alberto. “Especies del genéro Candida implicadas en estomatitis subprotésica de pacientes del Departamento de Odontoestomatología del Centro Médico Naval "CMST"-2007.” 2008. Web. 25 Feb 2020.

Vancouver:

Rojas Zumaeta LA. Especies del genéro Candida implicadas en estomatitis subprotésica de pacientes del Departamento de Odontoestomatología del Centro Médico Naval "CMST"-2007. [Internet] [Thesis]. National University of San Marcos; 2008. [cited 2020 Feb 25]. Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/2184 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F2184/1/bitstream.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rojas Zumaeta LA. Especies del genéro Candida implicadas en estomatitis subprotésica de pacientes del Departamento de Odontoestomatología del Centro Médico Naval "CMST"-2007. [Thesis]. National University of San Marcos; 2008. Available from: http://cybertesis.unmsm.edu.pe/handle/cybertesis/2184 ; http://cybertesis.unmsm.edu.pe/bitstream/cybertesis%2F2184/1/bitstream

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

11. Kiss, Ágnes. A Candida albicans környezeti stressz indukálta morfológiai változásai .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/118229

► A Candida albicans az emberi szervezetből leggyakrabban izolált opportunista patogén élesztőgomba, melynek környezeti stresszre adott morfológiai átalakulásait vizsgáltuk. Tanulmányok szerint a C. albicans a központi… (more)

Subjects/Keywords: Candida albicans; morfológia

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APA (6^th Edition):

Kiss, �. (2011). A Candida albicans környezeti stressz indukálta morfológiai változásai . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/118229

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kiss, Ágnes. “A Candida albicans környezeti stressz indukálta morfológiai változásai .” 2011. Thesis, University of Debrecen. Accessed February 25, 2020. http://hdl.handle.net/2437/118229.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kiss, Ágnes. “A Candida albicans környezeti stressz indukálta morfológiai változásai .” 2011. Web. 25 Feb 2020.

Vancouver:

Kiss �. A Candida albicans környezeti stressz indukálta morfológiai változásai . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2437/118229.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kiss �. A Candida albicans környezeti stressz indukálta morfológiai változásai . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/118229

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

12. Borák, Balázs Sándor. Candida albicans Time- lapse imaging képszekvenciáinak digitális képanalízise .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2013, University of Debrecen

URL: http://hdl.handle.net/2437/168010

► Munkánk során két Candida albicans törzs morfológiai sajátságát vizsgáltuk szérum, illetve szérummentes környezetbe. A felvételek a tanszéken dolgozó fiatalok által kifejlesztett sejttenyésztő inkubátorba elhelyezett 4… (more)

Subjects/Keywords: Candida albicans; LTS

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APA (6^th Edition):

Borák, B. S. (2013). Candida albicans Time- lapse imaging képszekvenciáinak digitális képanalízise . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/168010

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Borák, Balázs Sándor. “Candida albicans Time- lapse imaging képszekvenciáinak digitális képanalízise .” 2013. Thesis, University of Debrecen. Accessed February 25, 2020. http://hdl.handle.net/2437/168010.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Borák, Balázs Sándor. “Candida albicans Time- lapse imaging képszekvenciáinak digitális képanalízise .” 2013. Web. 25 Feb 2020.

Vancouver:

Borák BS. Candida albicans Time- lapse imaging képszekvenciáinak digitális képanalízise . [Internet] [Thesis]. University of Debrecen; 2013. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2437/168010.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Borák BS. Candida albicans Time- lapse imaging képszekvenciáinak digitális képanalízise . [Thesis]. University of Debrecen; 2013. Available from: http://hdl.handle.net/2437/168010

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

13. Sipos, Lilla. Betametazon hatása a Candida albicans virulenciájára .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2013, University of Debrecen

URL: http://hdl.handle.net/2437/168030

A gyógyászatban általánosan alkalmazott glükokortikoszteroid (betametazon) hatása az opportunista patogén Candida albicans dimorf gomba virulenciájára. Advisors/Committee Members: Pócsi, István (advisor).

Subjects/Keywords: Candida albicans; betametazon

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APA (6^th Edition):

Sipos, L. (2013). Betametazon hatása a Candida albicans virulenciájára . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/168030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sipos, Lilla. “Betametazon hatása a Candida albicans virulenciájára .” 2013. Thesis, University of Debrecen. Accessed February 25, 2020. http://hdl.handle.net/2437/168030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sipos, Lilla. “Betametazon hatása a Candida albicans virulenciájára .” 2013. Web. 25 Feb 2020.

Vancouver:

Sipos L. Betametazon hatása a Candida albicans virulenciájára . [Internet] [Thesis]. University of Debrecen; 2013. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2437/168030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sipos L. Betametazon hatása a Candida albicans virulenciájára . [Thesis]. University of Debrecen; 2013. Available from: http://hdl.handle.net/2437/168030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

14. Chaillot, Julien. Étude de l'homéostasie de la taille chez la levure opportuniste Candida albicans.

Degree: 2019, Université Laval

URL: http://hdl.handle.net/20.500.11794/35434

►

L’homéostasie de la taille est un processus important de la prolifération cellulaire mais les mécanismes moléculaires sont mal compris. Les cellules eucaryotes doivent atteindre une… (more)

▼

L’homéostasie de la taille est un processus important de la prolifération cellulaire mais les mécanismes moléculaires sont mal compris. Les cellules eucaryotes doivent atteindre une taille seuil avant la division, ce qui permet de maintenir une taille constante sur le long terme. Ce processus est régulé à un point de contrôle, à la fin de la phase G1, appelé START chez les levures. Le contrôle de la taille cellulaire a été étudié chez la levure modèle Saccharomyces cerevisiae mais n’a jamais été étudié chez les levures pathogènes. Dans cette thèse, nous avons utilisé Candida albicans comme organisme modèle pour étudier la régulation de la taille chez les levures opportunistes. Nous avons criblé des collections de mutants de délétions hétérozygotes et homozygotes de C.albicans afin d’identifier des gènes régulateurs de la taille. Nous avons analysé la distribution de taille de 279 mutants homozygotes et 4 348 mutants hétérozygotes (recouvrant 90% du génome). Nous avons comparé nos résultats à différents criblages effectués sur la levure modèle S.cerevisiae. Ces comparaisons montrent que peu de régulateurs sont conservés entre C. albicans et S. cerevisiae et que la régulation de la taille est processus très plastique. Par exemple, le mutant dot6 a un phénotype petit chez C. albicans mais n’a pas de phénotype de taille chez S. cerevisiae. Nous avons montré que Dot6 est un facteur de transcription nécessaire pour l’activation des gènes de la biogénèse des ribosomes. Dot6 est également un régulateur de START et joue un rôle dans l’adaptation de la taille suivant les sources de carbone disponibles. Nous avons également mis en évidence un nouveau rôle pour la protéine kinase Hog1/p38 dans la régulation de la taille chez C. albicans en absence de stress. Ce rôle n’a jamais été démontré chez S. cerevisiae. Nous avons montré que Hog1, ainsi que toute la voie HOG, sont des régulateurs négatifs de START. Nous avons mis en évidence que Hog1 régule à la fois la croissance cellulaire via Sfp1, un régulateur majeur de la biogénèse des ribosomes et des protéines ribosomales, et le cycle cellulaire via le complexe SBF (Swi4/Swi6), des facteurs de transcription nécessaires pour la transition G1/S. Nous avons également découvert qu’Ahr1, un facteur de transcription n’ayant pas d’orthologue chez S. cerevisiae, est nécessaire pour la régulation de la taille et aussi requis pour l’adaptation de la taille en fonction des acides aminés disponibles. Nous avons montré qu’Ahr1 agit dans la voie Tor1-Sch9 et régule négativement START. En conclusion, notre travail a permis de découvrir de nouveaux régulateurs de START, de caractériser leur fonction et de les placer dans différentes voies. Comme la dérégulation de la voie Hog1/p38 est associée à des pathologies humaines, nous proposons C.albicans comme organisme modèle pour l’étude de cette voie et son implication dans l’homéostasie de la taille chez les organismes eucaryotes.

Cell size homeostasis is an important process of cell proliferation but the molecular mechanisms are poorly understood.…

Advisors/Committee Members: Sellam, Adnane.

Subjects/Keywords: Homéostasie; Candida albicans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chaillot, J. (2019). Étude de l'homéostasie de la taille chez la levure opportuniste Candida albicans. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/35434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chaillot, Julien. “Étude de l'homéostasie de la taille chez la levure opportuniste Candida albicans.” 2019. Thesis, Université Laval. Accessed February 25, 2020. http://hdl.handle.net/20.500.11794/35434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chaillot, Julien. “Étude de l'homéostasie de la taille chez la levure opportuniste Candida albicans.” 2019. Web. 25 Feb 2020.

Vancouver:

Chaillot J. Étude de l'homéostasie de la taille chez la levure opportuniste Candida albicans. [Internet] [Thesis]. Université Laval; 2019. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/20.500.11794/35434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chaillot J. Étude de l'homéostasie de la taille chez la levure opportuniste Candida albicans. [Thesis]. Université Laval; 2019. Available from: http://hdl.handle.net/20.500.11794/35434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

15. Preechasuth, Kanya. Regulation and cell biology of chitin synthesis in Candida albicans.

Degree: PhD, 2013, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202781 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589503

► Chitin biosynthesis in the fungal cell wall is essential for cell viability. Chitin play roles in maintaining cellular integrity and up regulation of chitin synthesis… (more)

▼ Chitin biosynthesis in the fungal cell wall is essential for cell viability. Chitin play roles in maintaining cellular integrity and up regulation of chitin synthesis helps protect the cell from cell wall stresses or cell wall damaging antifungal agents such as caspofungin. In Candida albicans chitin is synthesised by four chitin synthases (Chs), Chs1, Chs2, Chs3, and Chs8. The biological function of Chs2 and Chs8 (class I chitin synthases) is less well understood. The major objective of this thesis was to understand the biological function of the class I chitin synthases. Chs2-YFP and Chs8-YFP showed a dynamic localisation at septation sites, which were first visualised as a bar which then contracted to a spot. This spot then separated into two spots, one on each sides of the septum. These two spots remained there until yeast cell separation, and remained at this location throughout several subsequent hyphal cell cycles. Chs2-YFP also localised to hyphal tips. The phenotype of a chs2Δchs8Δ double mutant was re-investigated using the propidium iodide. Intact dead germ tubes and hyphal tip lysis was observed in a chs2Δchs8Δ mutant cells. This suggested that Chs2 and Chs8 play a major role in the maintenance of hyphal tip integrity and polarised growth and perhaps a minor role in septum formation. Studies were also performed to assess whether phosphorylation regulated the localisation of Chs2-YFP. It was shown that the localisation of Chs2-YFP to septation sites was regulated by phosphorylation on S222. A version of Chs2-YFP that could not be phosphorylated (Chs2S222A-YFP) localised at the septa in lower amounts than the Chs2-YFP, and a version of Chs2-YFP that mimicked constitutive phosphorylation (Chs2S222E-YFP) localised normally. This suggested that phosphorylation of Chs2 on S222 facilitates the localisation of Chs2-YFP at septation sites, and that dephosphorylation is not required for this cellular localisation. In the presence of cell wall stresses (CaCl2/CFW) and caspofungin, more Chs2-YFP was observed and the average intensity of fluorescence of Chs2-YFP was higher in the presence of these stresses than in untreated cells.

Subjects/Keywords: 610; Chitin; Candida albicans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Preechasuth, K. (2013). Regulation and cell biology of chitin synthesis in Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202781 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589503

Chicago Manual of Style (16^th Edition):

Preechasuth, Kanya. “Regulation and cell biology of chitin synthesis in Candida albicans.” 2013. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202781 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589503.

MLA Handbook (7^th Edition):

Preechasuth, Kanya. “Regulation and cell biology of chitin synthesis in Candida albicans.” 2013. Web. 25 Feb 2020.

Vancouver:

Preechasuth K. Regulation and cell biology of chitin synthesis in Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2013. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202781 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589503.

Council of Science Editors:

Preechasuth K. Regulation and cell biology of chitin synthesis in Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2013. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202781 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589503

University of Aberdeen

16. Whibley, Natasha. The role of effector and regulatory helper T cells in a murine model of systemic Candida albicans infection.

Degree: PhD, 2013, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192231 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569637

► Diseases caused by fungi are increasing worldwide and are often associated with high mortality rates. In particular, the normally harmless commensal Candida albicans can cause… (more)

Subjects/Keywords: 610; Candida albicans; T-cells

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APA (6^th Edition):

Whibley, N. (2013). The role of effector and regulatory helper T cells in a murine model of systemic Candida albicans infection. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192231 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569637

Chicago Manual of Style (16^th Edition):

Whibley, Natasha. “The role of effector and regulatory helper T cells in a murine model of systemic Candida albicans infection.” 2013. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192231 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569637.

MLA Handbook (7^th Edition):

Whibley, Natasha. “The role of effector and regulatory helper T cells in a murine model of systemic Candida albicans infection.” 2013. Web. 25 Feb 2020.

Vancouver:

Whibley N. The role of effector and regulatory helper T cells in a murine model of systemic Candida albicans infection. [Internet] [Doctoral dissertation]. University of Aberdeen; 2013. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192231 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569637.

Council of Science Editors:

Whibley N. The role of effector and regulatory helper T cells in a murine model of systemic Candida albicans infection. [Doctoral Dissertation]. University of Aberdeen; 2013. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=192231 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.569637

University of Aberdeen

17. Okai, Blessing. The role of Rab14 in the maturation of macrophage phagosomes containing Candida albicans.

Degree: PhD, 2014, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=214847 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629398

► The virulence of the opportunistic fungal pathogen Candida albicans is in part due to its ability to switch between a yeast and hyphal form; permitting… (more)

▼ The virulence of the opportunistic fungal pathogen Candida albicans is in part due to its ability to switch between a yeast and hyphal form; permitting physical rupture and escape from macrophages after phagocytosis. This interesting feature makes C. albicans a good model organism to study in the context of phagosome maturation and in particular, the role of the small GTPase Rab14 in this process. Rab14 is recruited to bacterial phagosomes containing Chlamydia trachomatis (Capmany & Damiani, 2010) and Mycobacterium bovis BCG (Kyei et al, 2006) and aids their survival in macrophages but its role in phagosomes containing fungal pathogens is not known. Here, an important role for Rab14 in protecting macrophages against hyphal mediated lysis by C. albicans is demonstrated. Macrophages were transfected to express eGFP-Rab14, or dominant negative variants (eGFP-Rab14 S25N and eGFP-Rab14N124I); or were transfected with anti-Rab14 siRNA; then infected with live C. albicans and observed using sophisticated live cell imaging and analysis tools. Phagosomes containing live C. albicans became transiently Rab14-positive within 2 minutes following engulfment. Interestingly, a prolonged retention of Rab14 on phagosomes depended on C. albicans morphology. Phagosomes containing hyphal forms retained Rab14 for twice as long as for phagosomes containing the yeast form. Depletion of endogenous Rab14 did not affect macrophage migration towards C. albicans, the rate of engulfment or acquisition of markers of early phagosome maturation to phagosomes containing C. albicans. Furthermore, reduced Rab14 expression did not influence the kinetics of Rab5 localisation to phagosomes in macrophages that were co-transfected. Importantly, partially depleting Rab14 delayed the appearance of late phagosome maturation indicators LAMP1 and activated cathepsin in phagosomes containing live C. albicans.Rab14 knockdown was associated with a significant increase in macrophage killing by C. alibica The data presented in this thesis demonstrate the dynamic relationship between host and pathogen which can be visualised in real time at the level of individual phagosomes. Rab14 plays an important role during phagosome maturation which impacts on the later stages of phagosome maturation and is important for phagocyte survival after phagocytosis of C. albicans.

Subjects/Keywords: 610; Candida albicans; Macrophages; Phagosomes

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APA (6^th Edition):

Okai, B. (2014). The role of Rab14 in the maturation of macrophage phagosomes containing Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=214847 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629398

Chicago Manual of Style (16^th Edition):

Okai, Blessing. “The role of Rab14 in the maturation of macrophage phagosomes containing Candida albicans.” 2014. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=214847 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629398.

MLA Handbook (7^th Edition):

Okai, Blessing. “The role of Rab14 in the maturation of macrophage phagosomes containing Candida albicans.” 2014. Web. 25 Feb 2020.

Vancouver:

Okai B. The role of Rab14 in the maturation of macrophage phagosomes containing Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2014. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=214847 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629398.

Council of Science Editors:

Okai B. The role of Rab14 in the maturation of macrophage phagosomes containing Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2014. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=214847 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629398

University of Aberdeen

18. Mehrotra, Pankaj. Immunological and morphological characterization of Candida albicans and Candida haemulonii.

Degree: PhD, 2014, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210107 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606583

► During the infection process Candida albicans has to respond to various stresses imposed by host environment including oxidative and osmolarity stress generated by phagocytic cells… (more)

▼ During the infection process Candida albicans has to respond to various stresses imposed by host environment including oxidative and osmolarity stress generated by phagocytic cells such as macrophages and neutrophils. Exposure to caspofungin and other antifungal antibiotics also imposes stress on the C. albicans cell wall. These various stress responses are orchestrated through the activation of multiple stress pathways including the cAMP-PKA, several MAPK pathways and the Ca2+-calcineurin pathway which influence cell wall shape and composition. Such changes were predicted to influence recognition of C. albicans by innate immune cells. During my Ph.D. studies I primarily investigated the effect of the activation or inhibition of these pathways on the interaction with the innate immune cells by examining phagocytosis, the cytokine profile induced by mononuclear and polynuclear cells of the innate immune system. I found that the activation and inhibition of these pathways plays an important role in remodeling of cell wall and hence the immunological profile. Inactivation of cAMP, Calcium signaling pathway by the deletion of TPK1 and CNA1 resulted in marked reduction in pro-inflammatory cytokine production. Inactivation of MAPK pathway by deletion of HOG1 altered major pro-inflammatory cytokine secretion. Cytokine production was also affected by exposure of C. albicans signaling mutants to Calcofluor White, caspofungin, oxidative and osmotic inducing stresses. Cytokine stimulation was also affected by deletion of URA3, exposure of C. albicans to rifmapicin and antimycin A. These results suggest that stress signaling pathways act to regulate collateral changes in the cell wall, which in turn affects the immune reactivity. Pro and anti-inflammatory cytokine and antifungal profiles of Candida haemulonii was also found to be highly variable. Thus regulation and exposure to different microenvironments significantly modifies immunological signature of fungal cells, suggesting that responses to local stresses make the fungal cell surface a moving target for immunological surveillance.

Subjects/Keywords: 610; Candida albicans; Candidiasis

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APA (6^th Edition):

Mehrotra, P. (2014). Immunological and morphological characterization of Candida albicans and Candida haemulonii. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210107 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606583

Chicago Manual of Style (16^th Edition):

Mehrotra, Pankaj. “Immunological and morphological characterization of Candida albicans and Candida haemulonii.” 2014. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210107 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606583.

MLA Handbook (7^th Edition):

Mehrotra, Pankaj. “Immunological and morphological characterization of Candida albicans and Candida haemulonii.” 2014. Web. 25 Feb 2020.

Vancouver:

Mehrotra P. Immunological and morphological characterization of Candida albicans and Candida haemulonii. [Internet] [Doctoral dissertation]. University of Aberdeen; 2014. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210107 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606583.

Council of Science Editors:

Mehrotra P. Immunological and morphological characterization of Candida albicans and Candida haemulonii. [Doctoral Dissertation]. University of Aberdeen; 2014. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210107 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606583

University of Hong Kong

19. 杨{274757}{275534}; Yang, Haiping. Role of secreted aspartic proteases in Candida albicans pathogenesis.

Degree: PhD, 2015, University of Hong Kong

URL: Yang, H. [杨{274757}{275534}]. (2015). Role of secreted aspartic proteases in Candida albicans pathogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5576756 ; http://dx.doi.org/10.5353/th_b5576756 ; http://hdl.handle.net/10722/230616

► Candida. albicans is the key pathogen for oral candidiasis, and possesses comprehensive virulence mechanisms to cause fungal infection in a host under proper predisposing conditions.… (more)

▼ Candida. albicans is the key pathogen for oral candidiasis, and possesses comprehensive virulence mechanisms to cause fungal infection in a host under proper predisposing conditions. The secreted aspartic protease (Sap) family (which consists of 10 family members: Sap1-10) plays a critical role in the pathogenicity of Candida. Considering the increasing prevalence rate of candidiasis worldwide, understanding of Sap family’s pathogenicity mechanism becomes critical for the development of any new antifungal agents or therapies. This study mainly investigated the virulent mechanisms of Sap7 and Sap9 in Candida infection, as well as the synergistic effect of HIV protease inhibitors and fluconazole on antifungal treatment. The first part of this study was to investigate the role of C. albicans SAP7 in fungal invasion to oral epithelial cells. sap7Δ/Δ mutant strain was created using a PCR-based gene disruption method via homologous recombination. Compared the wild type strain with sap7Δ/Δ mutant strain, the results demonstrated that the deletion of SAP7 restrained C. albicans invasion to oral epithelial cells. C. albicans sap7Δ/Δ mutant strain significantly reduced the induced endocytosis by 47.8%. Approximate 25% attenuation of sap7Δ/Δ mutant strain was internalized into the oral epithelial cells, and 27% reduction of the release of lactate dehydrogenase was detected. Evaluation of reconstituted human epithelial (RHE) tissues showed a significant reduction in the number of attached and invaded fungal cells to oral epithelial tissues compared with the wild type strain. The data implied that SAP7 contributes to C. albicans invasion to oral epithelial cells. The second part of the study was to investigate the role of C. albicans SAP9 in hyphae formation and invasion ability. Hyphal development was examined by both liquid and solid hyphal inducing media. Compared the sap9Δ/Δ mutant strain with wild type strain, hyphal formation tests demonstrated that the yeast-to-hyphal morphological transition was deficient in the sap9Δ/Δ mutant strain. Besides, quantitative reverse transcription-PCR (qRT-PCR) revealed a significant down regulation of the expression of EFG1 (approximately 40%) in sap9Δ/Δ mutant strain. EFG1 is a transcription factor gene positively mediating hyphae formation in C. albicans. It was also found that approximately 70% reduced sap9Δ/Δ mutant strain was endocytosed by host cells and 40% attenuation of host cell damage caused by sap9Δ/Δ mutant strain, compared with the wild type strains. The data strongly suggested that C. albicans Sap9 is a novel hyphal associated factor via the cAMP-protein kinase A pathway, leading to invasion into the host cells and causing cell damage. The final part of the study was to investigate the synergistic antifungal effect of HIV protease inhibitors and fluconazole on C. albicans. The results showed that while the MIC of saquinavir and fluconazole required to suppress the candidal growth was 700 μM and 32.64μM respectively, the combination of saquinavir (12.5 μM) and fluconazole…

Subjects/Keywords: Aspartic proteinases; Candida albicans

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APA (6^th Edition):

杨{274757}{275534}; Yang, H. (2015). Role of secreted aspartic proteases in Candida albicans pathogenesis. (Doctoral Dissertation). University of Hong Kong. Retrieved from Yang, H. [杨{274757}{275534}]. (2015). Role of secreted aspartic proteases in Candida albicans pathogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5576756 ; http://dx.doi.org/10.5353/th_b5576756 ; http://hdl.handle.net/10722/230616

Chicago Manual of Style (16^th Edition):

杨{274757}{275534}; Yang, Haiping. “Role of secreted aspartic proteases in Candida albicans pathogenesis.” 2015. Doctoral Dissertation, University of Hong Kong. Accessed February 25, 2020. Yang, H. [杨{274757}{275534}]. (2015). Role of secreted aspartic proteases in Candida albicans pathogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5576756 ; http://dx.doi.org/10.5353/th_b5576756 ; http://hdl.handle.net/10722/230616.

MLA Handbook (7^th Edition):

杨{274757}{275534}; Yang, Haiping. “Role of secreted aspartic proteases in Candida albicans pathogenesis.” 2015. Web. 25 Feb 2020.

Vancouver:

杨{274757}{275534}; Yang H. Role of secreted aspartic proteases in Candida albicans pathogenesis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2015. [cited 2020 Feb 25]. Available from: Yang, H. [杨{274757}{275534}]. (2015). Role of secreted aspartic proteases in Candida albicans pathogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5576756 ; http://dx.doi.org/10.5353/th_b5576756 ; http://hdl.handle.net/10722/230616.

Council of Science Editors:

杨{274757}{275534}; Yang H. Role of secreted aspartic proteases in Candida albicans pathogenesis. [Doctoral Dissertation]. University of Hong Kong; 2015. Available from: Yang, H. [杨{274757}{275534}]. (2015). Role of secreted aspartic proteases in Candida albicans pathogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5576756 ; http://dx.doi.org/10.5353/th_b5576756 ; http://hdl.handle.net/10722/230616

Universidade Estadual de Campinas

20. Peron, Isabela Haddad, 1989-. Avaliação de suscetibilidade e estudo de gene de resistência de isolados clínicos de Candida albicans obtidos de espécimes clínicos no Hospital de Clínicas da UNICAMP .

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312497

► Resumo: Leveduras do gênero Candida são integrantes da microbiota do corpo humano e, quando há um desequilíbrio entre microbiota e hospedeiro, podem se tornar patógenos… (more)

▼ Resumo: Leveduras do gênero Candida são integrantes da microbiota do corpo humano e, quando há um desequilíbrio entre microbiota e hospedeiro, podem se tornar patógenos oportunistas, sendo C.albicans a espécie mais frequente. A candidíase sistêmica apresenta sintomatologia infecciosa localizada que se dissemina a outros órgãos por via hematogênica. O antifúngico fluconazol inibe a síntese de ergosterol ¿ componente essencial da membrana da célula fúngica ¿ e é amplamente utilizado para profilaxia e tratamento de infecções causadas por espécies de Candida. Enquanto algumas são geneticamente resistentes (C.krusei) ou facilmente adquirem resistência (C.glabrata) a fluconazol, não há muitos relatos de resistência em C.albicans. Porém, esta levedura pode desenvolver mecanismos de resistência a fluconazol, incluindo mutações no gene ERG11, codificador da produção da enzima que atua na biossíntese do ergosterol. O presente estudo realizou a caracterização do perfil de suscetibilidade frente a antifúngicos de isolados de C.albicans obtidos de hemoculturas, bem como a investigação da ocorrência de mutações no gene ERG11 de dois isolados de C.albicans (LIF-E10 e LIF 12560) que apresentaram resistência fenotípica in vitro a fluconazol. O estudo contou com 147 isolados de C.albicans provenientes de hemoculturas realizadas no Hospital de Clínicas/UNICAMP no período de 2006 a 2010 e também com os isolados LIF-E10 e LIF 12560, que são procedentes de outros materiais clínicos (escovado esofágico e cavidade bucal, respectivamente). Os isolados estavam previamente identificados segundo metodologia micológica manual e automação (equipamento Vitek® 2 bioMérieux). Foram reativados por meio de cultura em ágar Sabouraud dextrose e posteriormente depositados em painéis para identificação no equipamento BD Phoenix®. Os testes de suscetibilidade in vitro aos antifúngicos micafungina, anfotericina B, 5-flucitosina, fluconazol, voriconazol, itraconazol e caspofungina foram realizados por microdiluição em caldo segundo os documentos M27-A3 e M27-S4 do Clinical and Laboratory Standards Institute (CLSI). A triagem de mutações no gene ERG11 de LIF-E10 e LIF 12560 ocorreu pela amplificação de três regiões gênicas, com os iniciadores ERGSec1A/1B, ERGSec2A/2B e ERGSec3A/3B. O produto resultante foi submetido a sequenciamento e análise dos fragmentos de DNA amplificados por meio da comparação a uma cepa padrão suscetível a fluconazol (C.albicans ATCC 90028). Dos 147 isolados clínicos que passaram por reativação, 143 foram recuperados e quatro não apresentaram crescimento. Dos 143 submetidos à reidentificação no equipamento BD Phoenix®, 128 (89,5%) foram identificados como C.albicans e 15 (10,5%) foram identificados como Candida dubliniensis. Com o resultado divergente apresentado pelo BD Phoenix® em relação à identificação anterior feita pelo Vitek® 2, foi necessária a realização do sequenciamento (iniciadores ITS4/ITS5 e NL1/NL4), que resultou na confirmação dos 15 isolados como pertencentes à espécie C.albicans. Nos testes de suscetibilidade in… Advisors/Committee Members: Schreiber, Angélica Zaninelli, 1965- (advisor), Almeida, Wanda Pereira (committee member), Garlipp, Celia Regina (committee member).

Subjects/Keywords: Candida albicans; Antifúngicos; Candidemia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peron, Isabela Haddad, 1. (2016). Avaliação de suscetibilidade e estudo de gene de resistência de isolados clínicos de Candida albicans obtidos de espécimes clínicos no Hospital de Clínicas da UNICAMP . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/312497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Peron, Isabela Haddad, 1989-. “Avaliação de suscetibilidade e estudo de gene de resistência de isolados clínicos de Candida albicans obtidos de espécimes clínicos no Hospital de Clínicas da UNICAMP .” 2016. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Peron, Isabela Haddad, 1989-. “Avaliação de suscetibilidade e estudo de gene de resistência de isolados clínicos de Candida albicans obtidos de espécimes clínicos no Hospital de Clínicas da UNICAMP .” 2016. Web. 25 Feb 2020.

Vancouver:

Peron, Isabela Haddad 1. Avaliação de suscetibilidade e estudo de gene de resistência de isolados clínicos de Candida albicans obtidos de espécimes clínicos no Hospital de Clínicas da UNICAMP . [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2020 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Peron, Isabela Haddad 1. Avaliação de suscetibilidade e estudo de gene de resistência de isolados clínicos de Candida albicans obtidos de espécimes clínicos no Hospital de Clínicas da UNICAMP . [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Morgana Ramos Martins Xerez. Fator de necrose tumoral alfa (TNF-α) inibe a formaÃÃo do biofilme de C. albicans.

Degree: Master, 2016, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16602 ;

►

The aim of this study was to investigate the activity of TNF-α against Candida albicans biofilm. Therefore, week biofilm producer (49BMv and 51BMv) and strong… (more)

▼

The aim of this study was to investigate the activity of TNF-α against Candida albicans biofilm. Therefore, week biofilm producer (49BMv and 51BMv) and strong biofilm producer (ATCC 10231 and CEMM 01.05.006) strains of C. albicans were incubated in RPMI medium in 96-well plates at 35 ÂC for 48 or 96 hours in order to assess biofilm formation and mature biofilm, respectively, in the absence or presence of different concentrations of TNF-α (0.1, 0.5, 1, 10, 20 and 40 ng/ml). TNF-α was added during preparation of the biofilm to assess its effect on biofilm formation or after 48 hours of culture for evaluation in mature biofilm. After incubation, the metabolic activity was assessed using XTT reduction assay. The minimum inhibitory concentration (MIC) of TNF-α against C. albicans was evaluated by broth microdilution technique according to Document M27-A3, recommended by CLSI, using the concentrations 0.5, 1, 10 and 20 ng/mL. An analysis of the hyphae/blastoconidia ratio in biofilm was performed using optical microscopy by couting of filamentous (hyphae and pseudo-hyphae) and yeast cells and then a formula which allowed visualization of the relationship between the amount of filamentous and other structures was applied. The results indicated that TNF-α showed a significant inhibition of biofilm formation in a dose-dependent way and a discrete mature biofilm inhibition. In addition, the analysis of hyphae/blastoconidia ratio showed a statistically significant reduction in the number of filamentous forms in biofilm formation in the presence of TNF-α. However, the growth of C. albicans in planktonic form was not affected by any of the TNF-α concentrations used. These results indicate that TNF-α inhibits the formation of C. albicans biofilms, probably through the suppression of filamentation, a crucial step in biofilm formation.

O presente estudo teve como objetivo investigar a aÃÃo do TNF-α frente ao biofilme de Candida albicans. Para tanto, cepas de C. albicans fracamente produtoras de biofilme (49BMv e 51BMv) e fortemente produtoras de biofilme (ATCC 10231 e CEMM 01.05.006) foram incubadas em meio RPMI em placas de 96 poÃos, a 35 ÂC por 48 ou 96 horas para avaliar o desenvolvimento do biofilme em formaÃÃo e maduro, respectivamente, na ausÃncia ou presenÃa de diferentes concentraÃÃes de TNF-α (0,1, 0,5, 1, 10, 20 e 40 ng/mL). A adiÃÃo do TNF-α foi realizada no momento do preparo do cultivo para avaliar seu efeito no biofilme em formaÃÃo ou apÃs 48 horas de cultura para avaliaÃÃo no biofilme maduro. ApÃs incubaÃÃo, a atividade metabÃlica foi avaliada por meio do ensaio de reduÃÃo de XTT. A concentraÃÃo inibitÃria mÃnima (CIM) do TNF-α em C. albicans foi avaliada, dentre as concentraÃÃes testadas 0,5, 1, 10 e 20 ng/mL, por meio da tÃcnica de microdiluiÃÃo em caldo, segundo documento M27-A3, preconizada pelo CLSI. Foi realizada uma anÃlise da relaÃÃo hifas/blastoconÃdios do biofilme por microscopia Ãptica, por meio de uma contagem das formas filamentosas (hifas e…

Advisors/Committee Members: Francisco Airton Castro da Rocha, VirgÃnia ClÃudia Carneiro GirÃo, Marcos FÃbio Gadelha Rocha.

Subjects/Keywords: FARMACIA; Biofilmes; Candida albicans; Hifas

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APA (6^th Edition):

Xerez, M. R. M. (2016). Fator de necrose tumoral alfa (TNF-α) inibe a formaÃÃo do biofilme de C. albicans. (Masters Thesis). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16602 ;

Chicago Manual of Style (16^th Edition):

Xerez, Morgana Ramos Martins. “Fator de necrose tumoral alfa (TNF-α) inibe a formaÃÃo do biofilme de C. albicans.” 2016. Masters Thesis, Universidade Federal do Ceará. Accessed February 25, 2020. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16602 ;.

MLA Handbook (7^th Edition):

Xerez, Morgana Ramos Martins. “Fator de necrose tumoral alfa (TNF-α) inibe a formaÃÃo do biofilme de C. albicans.” 2016. Web. 25 Feb 2020.

Vancouver:

Xerez MRM. Fator de necrose tumoral alfa (TNF-α) inibe a formaÃÃo do biofilme de C. albicans. [Internet] [Masters thesis]. Universidade Federal do Ceará 2016. [cited 2020 Feb 25]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16602 ;.

Council of Science Editors:

Xerez MRM. Fator de necrose tumoral alfa (TNF-α) inibe a formaÃÃo do biofilme de C. albicans. [Masters Thesis]. Universidade Federal do Ceará 2016. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16602 ;

University of Aberdeen

22. Bohovych, Iryna M. Impact of glucose on oxidative stress resistance in Candida albicans.

Degree: 2012, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=186766 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558668

► Candida albicans, a successful human pathogen, displays the phenomenon of glucoseenhanced oxidative stress resistance (Rodaki et al., 2009), which is not observed in other yeast… (more)

▼ Candida albicans, a successful human pathogen, displays the phenomenon of glucoseenhanced oxidative stress resistance (Rodaki et al., 2009), which is not observed in other yeast species tested. The molecular bases of the phenomenon are not clear. It was suggested that glucose signalling might play a major role. Therefore, the impact of specific C. albicans mutations was tested to determine which of three known major glucose signalling pathways are required for glucose-enhanced oxidative stress resistance. Two major glucose signalling pathways were found to contribute to the phenomenon (the glucose repression pathway and the cAMP pathway), and a third pathway (the SRR pathway) is not essential for this response. The next step was to identify targets of these pathways that might contribute to the phenotype. First, it was tested whether known oxidative stress systems contribute to the GEXSR. The selected targets represented almost all main systems involved in redox control and ROS detoxification (catalase, superoxide dismutases, thiredoxins, and glutathione peroxidases) which seemed to contribute not significantly to the GEXSR. The exceptions to this were glutathione reductase (Glr1) and glutaredoxin (Grx2/Ttr1), inactivation of which affected manifestation of the phenomenon. This reinforced the view that the GSH/GSSG balance plays a key role in the GEXSR. Second, comparative analyses of transcriptomic profiles of C. albicans glucose- and lactategrown cells in response to oxidative stress and glucose treatment correspondingly revealed a small set of commonly up-regulated genes: UCF1, RNR22, MOH1, orf19.3302, and HSP21/orf19.822 (Enjalbert et al., 2006; Rodaki et al., 2009). Each potential GEXSR-specific gene appeared to be regulated in a distinct manner by the major glucose signalling pathways. The ectopic expression of potential GEXSR targets did not provide any experimental evidence to support their roles in this response. That might be related to inefficient expression from ACT1 promoter under the experimental conditions tested, and also caused by other effects.

Subjects/Keywords: 616.96901; Candida Albicans; Glucose

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APA (6^th Edition):

Bohovych, I. M. (2012). Impact of glucose on oxidative stress resistance in Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=186766 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558668

Chicago Manual of Style (16^th Edition):

Bohovych, Iryna M. “Impact of glucose on oxidative stress resistance in Candida albicans.” 2012. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=186766 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558668.

MLA Handbook (7^th Edition):

Bohovych, Iryna M. “Impact of glucose on oxidative stress resistance in Candida albicans.” 2012. Web. 25 Feb 2020.

Vancouver:

Bohovych IM. Impact of glucose on oxidative stress resistance in Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2012. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=186766 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558668.

Council of Science Editors:

Bohovych IM. Impact of glucose on oxidative stress resistance in Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2012. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=186766 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558668

University of Aberdeen

23. McKenzie, Christopher Gordon Jemison. Candida albicans recognition by and escape from macrophages.

Degree: 2011, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167784 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.542642

► Disruption of N-mannosylation and O-mannosylation on the C. albicans outer cell wall increased the rate by which C. albicans is ingested by macrophages. Conversely, disruption… (more)

Subjects/Keywords: 616.9; Candida Albicans : Macrophages

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APA (6^th Edition):

McKenzie, C. G. J. (2011). Candida albicans recognition by and escape from macrophages. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167784 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.542642

Chicago Manual of Style (16^th Edition):

McKenzie, Christopher Gordon Jemison. “Candida albicans recognition by and escape from macrophages.” 2011. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167784 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.542642.

MLA Handbook (7^th Edition):

McKenzie, Christopher Gordon Jemison. “Candida albicans recognition by and escape from macrophages.” 2011. Web. 25 Feb 2020.

Vancouver:

McKenzie CGJ. Candida albicans recognition by and escape from macrophages. [Internet] [Doctoral dissertation]. University of Aberdeen; 2011. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167784 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.542642.

Council of Science Editors:

McKenzie CGJ. Candida albicans recognition by and escape from macrophages. [Doctoral Dissertation]. University of Aberdeen; 2011. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167784 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.542642

University of Aberdeen

24. Walker, Louise. Dynamic responses of the fungal cell wall to stress and antifungal treatment.

Degree: 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=136783 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522067

► The main aim of this project was to determine the potential of increased chitin content as a mechanism of resistance to caspofungin in different fungal… (more)

Subjects/Keywords: 616.9; Chitin : Candida Albicans

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APA (6^th Edition):

Walker, L. (2010). Dynamic responses of the fungal cell wall to stress and antifungal treatment. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=136783 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522067

Chicago Manual of Style (16^th Edition):

Walker, Louise. “Dynamic responses of the fungal cell wall to stress and antifungal treatment.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=136783 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522067.

MLA Handbook (7^th Edition):

Walker, Louise. “Dynamic responses of the fungal cell wall to stress and antifungal treatment.” 2010. Web. 25 Feb 2020.

Vancouver:

Walker L. Dynamic responses of the fungal cell wall to stress and antifungal treatment. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=136783 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522067.

Council of Science Editors:

Walker L. Dynamic responses of the fungal cell wall to stress and antifungal treatment. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=136783 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522067

University of Aberdeen

25. Sandai, Doblin Anak. The regulation of carbon assimilation in Candida albicans.

Degree: 2011, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203832 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540454

► The main objective of this thesis was to study the effects of glucose on the regulation central carbon metabolic functions in the human fungal pathogen… (more)

▼ The main objective of this thesis was to study the effects of glucose on the regulation central carbon metabolic functions in the human fungal pathogen Candida albicans (C. albicans). The virulence of C. albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility (Brown, 2005). The assimilation of carbon sources is fundamentally important for growth in all organisms and essential for the establishment of infections by pathogens, such as C. albicans in their human host. The C. albicans PCK1 and ICL1 genes, which encode the gluconeogenic and glyoxylate cycle enzymes phosphoenolpyruvate carboxykinase and isocitrate lyase are required for growth on non-fermentable carbon sources such as lactate and oleic acid. This thesis examines the impact of glucose upon the assimilation of secondary carbon sources such as lactate and oleic acid by C. albicans. The addition of 2% glucose repressed the CaPCK1 and CaICL1 genes. However, the enzymes CaIcl1 and CaPck1 were not destabilised by glucose and they retained at high levels following glucose addition. As a result, C. albicans cells continued to assimilate lactate and oleic acid in the presence of glucose. In contrast, the ScPck1 and ScIcl1 proteins were degraded rapidly in S. cerevisiae, and lactate and oleic acid assimilation was repressed in response to glucose. Therefore, while C. albicans and S. cerevisiae display similar responses to glucose at the transcriptional level, their responses at post-transcriptional and metabolic level diverge significantly. As a result, C. albicans can assimilate both glucose and alternative carbon sources at the same time. Next, the molecular apparatus that triggers the destabilisation of target proteins in response to glucose in C. albicans was studied. The expression of C. albicans the ICLI ORF in S. cerevisiae suggested that CaIcl1 has lost the molecular signal that triggers destabilisation in response to glucose, as CaIcl1 was not degraded in response to glucose in S. cerevisiae. However, when ScIcl1 was expressed in C. albicans ScIcl1 was rapidly degraded in response to glucose indicating that C. albicans has retained the molecular apparatus for glucose-accelerated degradation of target proteins. ScIcl1 degradation was slowed in C. albicans ubi4/ubi4 mutants. Furthermore, the addition of a putative of S. cerevisiae ubiquitination site carboxy terminus of CaIcl1 led to glucose-accelerated degradation of this protein in C. albicans cells. Therefore, glucose triggers accelerated degradation of target proteins in C. albicans via a ubiquitin-dependent process.

Subjects/Keywords: 571.29; Candida albicans : Carbon

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sandai, D. A. (2011). The regulation of carbon assimilation in Candida albicans. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203832 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540454

Chicago Manual of Style (16^th Edition):

Sandai, Doblin Anak. “The regulation of carbon assimilation in Candida albicans.” 2011. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203832 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540454.

MLA Handbook (7^th Edition):

Sandai, Doblin Anak. “The regulation of carbon assimilation in Candida albicans.” 2011. Web. 25 Feb 2020.

Vancouver:

Sandai DA. The regulation of carbon assimilation in Candida albicans. [Internet] [Doctoral dissertation]. University of Aberdeen; 2011. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203832 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540454.

Council of Science Editors:

Sandai DA. The regulation of carbon assimilation in Candida albicans. [Doctoral Dissertation]. University of Aberdeen; 2011. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203832 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540454

Montana State University

26. Hazen, Kevin Clark. Isolation of a germination autoinhibitor produced by Candida albicans.

Degree: College of Agriculture, 1982, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/3912

Subjects/Keywords: Candida albicans.

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APA (6^th Edition):

Hazen, K. C. (1982). Isolation of a germination autoinhibitor produced by Candida albicans. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/3912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hazen, Kevin Clark. “Isolation of a germination autoinhibitor produced by Candida albicans.” 1982. Thesis, Montana State University. Accessed February 25, 2020. https://scholarworks.montana.edu/xmlui/handle/1/3912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hazen, Kevin Clark. “Isolation of a germination autoinhibitor produced by Candida albicans.” 1982. Web. 25 Feb 2020.

Vancouver:

Hazen KC. Isolation of a germination autoinhibitor produced by Candida albicans. [Internet] [Thesis]. Montana State University; 1982. [cited 2020 Feb 25]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hazen KC. Isolation of a germination autoinhibitor produced by Candida albicans. [Thesis]. Montana State University; 1982. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

27. Glee, Pati Maurene. Molecular genetics of a Candida albicans-specific DNA fragment.

Degree: College of Agriculture, 1992, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/7163

Subjects/Keywords: Candida albicans.

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APA (6^th Edition):

Glee, P. M. (1992). Molecular genetics of a Candida albicans-specific DNA fragment. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/7163

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Glee, Pati Maurene. “Molecular genetics of a Candida albicans-specific DNA fragment.” 1992. Thesis, Montana State University. Accessed February 25, 2020. https://scholarworks.montana.edu/xmlui/handle/1/7163.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Glee, Pati Maurene. “Molecular genetics of a Candida albicans-specific DNA fragment.” 1992. Web. 25 Feb 2020.

Vancouver:

Glee PM. Molecular genetics of a Candida albicans-specific DNA fragment. [Internet] [Thesis]. Montana State University; 1992. [cited 2020 Feb 25]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/7163.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Glee PM. Molecular genetics of a Candida albicans-specific DNA fragment. [Thesis]. Montana State University; 1992. Available from: https://scholarworks.montana.edu/xmlui/handle/1/7163

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

28. Li, Ren-Kai. Structural determination and function of a cell wall epitope of Candida albicans.

Degree: College of Agriculture, 1992, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/6709

Subjects/Keywords: Candida albicans.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, R. (1992). Structural determination and function of a cell wall epitope of Candida albicans. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/6709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Ren-Kai. “Structural determination and function of a cell wall epitope of Candida albicans.” 1992. Thesis, Montana State University. Accessed February 25, 2020. https://scholarworks.montana.edu/xmlui/handle/1/6709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Ren-Kai. “Structural determination and function of a cell wall epitope of Candida albicans.” 1992. Web. 25 Feb 2020.

Vancouver:

Li R. Structural determination and function of a cell wall epitope of Candida albicans. [Internet] [Thesis]. Montana State University; 1992. [cited 2020 Feb 25]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/6709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li R. Structural determination and function of a cell wall epitope of Candida albicans. [Thesis]. Montana State University; 1992. Available from: https://scholarworks.montana.edu/xmlui/handle/1/6709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

29. Garcia-Rangel, Carlos-Enrique. Anti-Candida activity of the human gut metabolome.

Degree: 2018, Université Laval

URL: http://hdl.handle.net/20.500.11794/29994

►

L’intestin humain contient une variété de microbes commensaux qui sont représentés par divers organismes appartenant aux trois domaines de la vie où les Eukarya sont… (more)

▼

L’intestin humain contient une variété de microbes commensaux qui sont représentés par divers organismes appartenant aux trois domaines de la vie où les Eukarya sont essentiellement représentés par le règne des champignons. La levure commensale et opportuniste Candida albicans a été identifiée comme étant le champignon le plus commun dans l’intestin des humains sains. Des études récentes soutiennent que malgré leur faible abondance les levures du genre Candida peuvent altérer l'équilibre du microbiote et conduire à des dysbioses ou des pathologies récurrentes comme la maladie de Crohn et les colites ulcéreuses. Il a été démontré que le microbiote commensal joue un rôle essentiel dans la protection de l’intestin contre la colonisation par des bactéries pathogènes et des pathobiontes. Cependant, jusqu'à présent, on ignore si la prolifération ou la pathogénicité de C. albicans peuvent être contrôlées par d'autres microbiotes fécaux. Dans cette étude, nous avons démontré que le métabolome microbien de l'intestin humain exerce une activité antifongique contre C. albicans et d’autres levures qui résident au niveau intestinal. Ces métabolites inhibent plusieurs traits de virulence de C. albicans incluant la filamentation et l'invasion des cellules humaines. De plus, un crible génétique chez C. albicans a suggéré que TOR est la cible moléculaire de la ou des molécules antifongiques du métabolome microbien de l'intestin humain.

The human gut contains a variety of commensal microbes which are composed of diverse organisms that belong to all three domains of life with Eukarya primarily represented by fungi. The commensal / opportunistic yeast Candida albicans has been reported as the most common fungus in the gut of healthy humans. Recent evidences support that, this small fraction can alter the microbiota equilibrium leading to dysbiosis and diseases like inflammatory bowel diseases. It has been demonstrated that commensal microbiota plays a critical role in the protection of the gut against colonization by other bacterial pathogens and pathobionts. However, so far, whether C. albicans overgrowth or pathogenicity are controlled by other fecal microbiota is not known. In this study, we showed that the human microbial gut metabolome (GM) exerts an antifungal activity against different intestinal-resident yeasts including hyphal growth and the invasion of human enterocytes of C. albicans. Furthermore, a genetic screen in C. albicans suggested that TOR is the molecular target of the antifungal molecule(s) of the GM.

Advisors/Committee Members: Sellam, Adnane.

Subjects/Keywords: Intestins – Microbiologie; Candida albicans; Métabolomique

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APA (6^th Edition):

Garcia-Rangel, C. (2018). Anti-Candida activity of the human gut metabolome. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/29994

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Garcia-Rangel, Carlos-Enrique. “Anti-Candida activity of the human gut metabolome.” 2018. Thesis, Université Laval. Accessed February 25, 2020. http://hdl.handle.net/20.500.11794/29994.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Garcia-Rangel, Carlos-Enrique. “Anti-Candida activity of the human gut metabolome.” 2018. Web. 25 Feb 2020.

Vancouver:

Garcia-Rangel C. Anti-Candida activity of the human gut metabolome. [Internet] [Thesis]. Université Laval; 2018. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/20.500.11794/29994.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Garcia-Rangel C. Anti-Candida activity of the human gut metabolome. [Thesis]. Université Laval; 2018. Available from: http://hdl.handle.net/20.500.11794/29994

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

30. Nather, Kerstin. Dynamics and heterogeneity of Candida albicans cell surface proteins.

Degree: Dept. of Molecular and Cell Biology., 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158587 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158587&custom_att_2=simple_viewer

Subjects/Keywords: Candida Albicans.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nather, K. (2010). Dynamics and heterogeneity of Candida albicans cell surface proteins. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158587 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158587&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Nather, Kerstin. “Dynamics and heterogeneity of Candida albicans cell surface proteins.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158587 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158587&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Nather, Kerstin. “Dynamics and heterogeneity of Candida albicans cell surface proteins.” 2010. Web. 25 Feb 2020.

Vancouver:

Nather K. Dynamics and heterogeneity of Candida albicans cell surface proteins. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158587 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158587&custom_att_2=simple_viewer.

Council of Science Editors:

Nather K. Dynamics and heterogeneity of Candida albicans cell surface proteins. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158587 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158587&custom_att_2=simple_viewer

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