subject:(Calcium)

1. Inglebert, Yanis. Règle de STDP en calcium physiologique : STDP rules in physiological calcium.

Degree: Docteur es, Neurosciences, 2018, Aix Marseille Université

URL: http://www.theses.fr/2018AIXM0642

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L’utilisation de calcium en concentration physiologique pourrait diminuer ou supprimer tout phénomène de plasticité induite par la règle de STDP avec des protocoles standards. Dans… (more)

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L’utilisation de calcium en concentration physiologique pourrait diminuer ou supprimer tout phénomène de plasticité induite par la règle de STDP avec des protocoles standards. Dans la région CA1 de l’hippocampe, pour une concentration de calcium de 1.8 mM, nous observons une absence de LTP après corrélation positive, mais une LTD pour l’ensemble des délais étudiés. Pour 1.3 mM, ni LTP ni LTD ne sont observées dans nos conditions expérimentales. En plus du calcium, l’activité est une composante importante dans l’induction de la plasticité par STDP. En particulier, l’augmentation de la fréquence de stimulation pendant l’appariement ou l’augmentation du nombre de potentiels d’action post-synaptiques nous a permis de récupérer la LTP par corrélation positive à 1.3 et 1.8 mM. De la même manière, l’augmentation du nombre de potentiels d’action post-synaptiques ou de la fréquence de stimulation nous a permis de récupérer la fenêtre de LTD à 1.3 mM. Parallèlement à la modulation d’activité, nous avons testé un troisième facteur qui apparaît important : la neuromodulation. La règle de STDP serait modulée, en particulier, par l’activation des récepteurs dopaminergiques et noradrénergiques. La perfusion d’isoprénaline, un agoniste des récepteurs noradrénergiques, nous a permis de récupérer la fenêtre de LTP à 1.8 mM, contrairement à l’application de dopamine. Mes résultats montrent que la règle de STDP est profondément modifiée en calcium physiologique, mais que l’utilisation d’activités spécifiques ou l’application de neuromodulateurs sont capables de restaurer un profil de courbe normal.

The use of calcium in physiological concentration may be able to reduce or eliminate any plasticity phenomenon induced by the STDP rule with standard protocols. In the CA1 region of the hippocampus, at a calcium concentration of 1.8 mM we observed an absence of LTP after positive correlation. Instead, LTD is observed for all the delays that were tested. At 1.3 mM, neither LTP nor LTD were observed under our experimental conditions. In addition to calcium, activity is an important component in the induction of plasticity by STDP. Notably, an increase in stimulation frequency during pairing or an increase in the number of postsynaptic action potential allowed us to rescue LTP induced by positive correlation at 1.3 and 1.8 mM. Similarly, an increase in the number of postsynaptic action potentials or the frequency of stimulation allowed us to rescue the LTD window at 1.3 mM. In parallel with activity modulation, we tested a third factor that showed a noticeable impact: neuromodulation. The STDP rule appeared to be predominantly modulated by the activation of dopaminergic and noradrenergic receptors. The perfusion of Isoprenaline, a noradrenergic receptor agonist, allowed us to rescue the LTP window whereas dopamine application at 1.8 mM did not rescue LTP. This study demonstrates that the STDP rule is profoundly changed under physiological calcium conditions; however, the use of specific activities or the application of neuromodulators…

Advisors/Committee Members: Debanne, Dominique (thesis director).

Subjects/Keywords: Calcium; Calcium

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APA (6^th Edition):

Inglebert, Y. (2018). Règle de STDP en calcium physiologique : STDP rules in physiological calcium. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2018AIXM0642

Chicago Manual of Style (16^th Edition):

Inglebert, Yanis. “Règle de STDP en calcium physiologique : STDP rules in physiological calcium.” 2018. Doctoral Dissertation, Aix Marseille Université. Accessed February 26, 2021. http://www.theses.fr/2018AIXM0642.

MLA Handbook (7^th Edition):

Inglebert, Yanis. “Règle de STDP en calcium physiologique : STDP rules in physiological calcium.” 2018. Web. 26 Feb 2021.

Vancouver:

Inglebert Y. Règle de STDP en calcium physiologique : STDP rules in physiological calcium. [Internet] [Doctoral dissertation]. Aix Marseille Université 2018. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2018AIXM0642.

Council of Science Editors:

Inglebert Y. Règle de STDP en calcium physiologique : STDP rules in physiological calcium. [Doctoral Dissertation]. Aix Marseille Université 2018. Available from: http://www.theses.fr/2018AIXM0642

University of Texas Southwestern Medical Center

2. Yao, Jian. Ca2+ Signaling in the Near Plasma Membrane Microdomain of Non-Excitable Cells.

Degree: 2011, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/864

► Calcium is the most versatile second messenger and plays fundamental roles in orchestrating enzyme secretion in exocrine acinar cells. Previous studies in excitable cells demonstrated… (more)

▼ Calcium is the most versatile second messenger and plays fundamental roles in orchestrating enzyme secretion in exocrine acinar cells. Previous studies in excitable cells demonstrated the existence of high Ca2+ microdomains. The major function of such microdomains is to create high local calcium concentration to activate various calcium-dependent signaling events. However, in non-excitable cells, direct evidence of such microdomains is absent. The goal of my study is to characterize the properties of high Ca2+ microdomains in acinar cells and explore its physiological relevance in the context of the secretory functions. By combining Total Internal Reflection Fluorescence Microscopy (TIRFM) technique and wide-field fluorescence imaging, I was able to quantify and compare changes in the concentration of free Ca2+ in the near membrane microdomains (?[Ca2+]PM) and in the bulk cytosol (?[Ca2+]Cyto). ?[Ca2+]PM is about 3-fold larger than ?[Ca2+]Cyto under maximal agonist stimulation, while resting [Ca2+]PM and [Ca2+]Cyto shows no difference. Near membrane microdomains also showed greater Ca2+ influx following store depletion induced either by activating surface receptor or by inhibiting SERCA pump. In response to physiological strength of stimulation, Ca2+ oscillation in the two compartments showed significantly different dynamics. The activation mechanisms of the Ca2+-induced Ca2+ release (CICR) are well established in cardiac and skeletal muscles and involves high Ca2+ microdomains. My study was the first to demonstrate the presence of CICR in the parotid acinar cell. In these cells, minimal activation of Ca2+ influx by partially depleting the stores, either by directly activating the cell surface receptor or by inhibiting SERCA, leads to an explosive release of Ca2+ from the majority of the stores, mediated presumably by RyR away from microdomains. The last part of my study is on the effects of chronic ER stress on Ca2+ signaling. The study suggests that ER stress induced by PERK mutation impeded both the efficiency and fidelity of Ca2+ signaling. My work validates the existence of near plasma membrane microdomains in non-excitable exocrine cells. The fact that [Ca2+]PM and [Ca2+]Cyto differ in many ways suggests that microdomains is the central signaling platform in these cells. Advisors/Committee Members: Bezprozvanny, Ilya.

Subjects/Keywords: Calcium; Calcium Channels; Portoid Gland

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APA (6^th Edition):

Yao, J. (2011). Ca2+ Signaling in the Near Plasma Membrane Microdomain of Non-Excitable Cells. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/864

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yao, Jian. “Ca2+ Signaling in the Near Plasma Membrane Microdomain of Non-Excitable Cells.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed February 26, 2021. http://hdl.handle.net/2152.5/864.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yao, Jian. “Ca2+ Signaling in the Near Plasma Membrane Microdomain of Non-Excitable Cells.” 2011. Web. 26 Feb 2021.

Vancouver:

Yao J. Ca2+ Signaling in the Near Plasma Membrane Microdomain of Non-Excitable Cells. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2152.5/864.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yao J. Ca2+ Signaling in the Near Plasma Membrane Microdomain of Non-Excitable Cells. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/864

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Nixon, Andrew T L. On Numerical Models of Calcium-Induced Calcium Release.

Degree: PhD, Applied Mathematics, 2015, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:419475/

► In this thesis, we examine hybrid numerical models of calcium-induced calcium release using deterministic PDEs for the diffusion of cytosolic calcium and a stochastic process… (more)

Subjects/Keywords: calcium-induced calcium release

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APA (6^th Edition):

Nixon, A. T. L. (2015). On Numerical Models of Calcium-Induced Calcium Release. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:419475/

Chicago Manual of Style (16^th Edition):

Nixon, Andrew T L. “On Numerical Models of Calcium-Induced Calcium Release.” 2015. Doctoral Dissertation, Brown University. Accessed February 26, 2021. https://repository.library.brown.edu/studio/item/bdr:419475/.

MLA Handbook (7^th Edition):

Nixon, Andrew T L. “On Numerical Models of Calcium-Induced Calcium Release.” 2015. Web. 26 Feb 2021.

Vancouver:

Nixon ATL. On Numerical Models of Calcium-Induced Calcium Release. [Internet] [Doctoral dissertation]. Brown University; 2015. [cited 2021 Feb 26]. Available from: https://repository.library.brown.edu/studio/item/bdr:419475/.

Council of Science Editors:

Nixon ATL. On Numerical Models of Calcium-Induced Calcium Release. [Doctoral Dissertation]. Brown University; 2015. Available from: https://repository.library.brown.edu/studio/item/bdr:419475/

Oregon State University

4. Redelings, Elizabeth Abbott. A determination of calcium in the diets of a cooperative house and a dormitory at Oregon State College.

Degree: MS, Foods and Nutrition, 1942, Oregon State University

URL: http://hdl.handle.net/1957/26461

Subjects/Keywords: Calcium

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APA (6^th Edition):

Redelings, E. A. (1942). A determination of calcium in the diets of a cooperative house and a dormitory at Oregon State College. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/26461

Chicago Manual of Style (16^th Edition):

Redelings, Elizabeth Abbott. “A determination of calcium in the diets of a cooperative house and a dormitory at Oregon State College.” 1942. Masters Thesis, Oregon State University. Accessed February 26, 2021. http://hdl.handle.net/1957/26461.

MLA Handbook (7^th Edition):

Redelings, Elizabeth Abbott. “A determination of calcium in the diets of a cooperative house and a dormitory at Oregon State College.” 1942. Web. 26 Feb 2021.

Vancouver:

Redelings EA. A determination of calcium in the diets of a cooperative house and a dormitory at Oregon State College. [Internet] [Masters thesis]. Oregon State University; 1942. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1957/26461.

Council of Science Editors:

Redelings EA. A determination of calcium in the diets of a cooperative house and a dormitory at Oregon State College. [Masters Thesis]. Oregon State University; 1942. Available from: http://hdl.handle.net/1957/26461

Oregon State University

5. Beals, Maple Cole. Food sources of calcium : The determination of calcium in meat cooked with acid.

Degree: MS, Foods and Nutrition, 1935, Oregon State University

URL: http://hdl.handle.net/1957/26248

Subjects/Keywords: Calcium

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APA (6^th Edition):

Beals, M. C. (1935). Food sources of calcium : The determination of calcium in meat cooked with acid. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/26248

Chicago Manual of Style (16^th Edition):

Beals, Maple Cole. “Food sources of calcium : The determination of calcium in meat cooked with acid.” 1935. Masters Thesis, Oregon State University. Accessed February 26, 2021. http://hdl.handle.net/1957/26248.

MLA Handbook (7^th Edition):

Beals, Maple Cole. “Food sources of calcium : The determination of calcium in meat cooked with acid.” 1935. Web. 26 Feb 2021.

Vancouver:

Beals MC. Food sources of calcium : The determination of calcium in meat cooked with acid. [Internet] [Masters thesis]. Oregon State University; 1935. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1957/26248.

Council of Science Editors:

Beals MC. Food sources of calcium : The determination of calcium in meat cooked with acid. [Masters Thesis]. Oregon State University; 1935. Available from: http://hdl.handle.net/1957/26248

Oregon State University

6. Olson, Eric J. Calcium in the equatorial Pacific Ocean.

Degree: MS, Oceanography, 1981, Oregon State University

URL: http://hdl.handle.net/1957/27993

Subjects/Keywords: Calcium

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APA (6^th Edition):

Olson, E. J. (1981). Calcium in the equatorial Pacific Ocean. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/27993

Chicago Manual of Style (16^th Edition):

Olson, Eric J. “Calcium in the equatorial Pacific Ocean.” 1981. Masters Thesis, Oregon State University. Accessed February 26, 2021. http://hdl.handle.net/1957/27993.

MLA Handbook (7^th Edition):

Olson, Eric J. “Calcium in the equatorial Pacific Ocean.” 1981. Web. 26 Feb 2021.

Vancouver:

Olson EJ. Calcium in the equatorial Pacific Ocean. [Internet] [Masters thesis]. Oregon State University; 1981. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1957/27993.

Council of Science Editors:

Olson EJ. Calcium in the equatorial Pacific Ocean. [Masters Thesis]. Oregon State University; 1981. Available from: http://hdl.handle.net/1957/27993

7. Blaeser, Andrew S. Optical Interrogation of the Spontaneous Dynamics of Prefrontal Cortical Networks.

Degree: PhD, Physics, 2015, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:419386/

► The prefrontal cortex (PFC) is the brain region most strongly associated with many higher cognitive functions such as working memory and decision making. While decades… (more)

▼ The prefrontal cortex (PFC) is the brain region most strongly associated with many higher cognitive functions such as working memory and decision making. While decades of study have produced a wealth of data about the anatomy and physiology of the PFC, our knowledge of the mechanisms by which the PFC stores, represents and processes information is currently incomplete. Specifically, little is known about the neuronal microcircuits within the PFC that may represent the basic units underlying its various functions. A deeper understanding of the network activity of PFC will likely be critically important for understanding cognition, and for developing therapies for PFC-related disorders such as schizophrenia. The advent of functional imaging has enabled the simultaneous measurement of the activity of large numbers of neighboring neurons, setting the stage for analysis of cortical microcircuits on a spatial scale that is inaccessible by traditional electrophysiological methods. In this thesis, we use calcium imaging in acute brain slices to characterize the dynamics of networks within deep-layer medial PFC. Taking advantage of the brain slice preparation, we use pharmacological agents to manipulate two molecular systems strongly implicated in network activity: NMDA receptors and GABAA receptors. We also present a framework for analyzing the resulting movies. Whereas the majority of neurons show only sporadic activity, a subset of neurons emit spontaneous, delta-band rhythmic activity. Looking beyond the activity of individual neurons, we examine the interactions between neurons. We find that spontaneous activity under baseline conditions is weakly correlated between pairs of neurons, and that rhythmic neurons showed little coherence in their oscillations. However, we consistently observed brief bouts of synchronous activity. Using surrogate data sets, we showed that the degree of correlation and synchrony we observed could not be explained by coincidence, but must be attributed partly to network activity. Finally, we described the effects of NMDA and picrotoxin on these metrics of network dynamics, and discuss possible implications of these results for understanding the neuronal bases of cognition and brain diseases. Advisors/Committee Members: Nurmikko, Arto (Director), Stein, Derek (Reader), Connors, Barry (Reader).

Subjects/Keywords: calcium imaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Blaeser, A. S. (2015). Optical Interrogation of the Spontaneous Dynamics of Prefrontal Cortical Networks. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:419386/

Chicago Manual of Style (16^th Edition):

Blaeser, Andrew S. “Optical Interrogation of the Spontaneous Dynamics of Prefrontal Cortical Networks.” 2015. Doctoral Dissertation, Brown University. Accessed February 26, 2021. https://repository.library.brown.edu/studio/item/bdr:419386/.

MLA Handbook (7^th Edition):

Blaeser, Andrew S. “Optical Interrogation of the Spontaneous Dynamics of Prefrontal Cortical Networks.” 2015. Web. 26 Feb 2021.

Vancouver:

Blaeser AS. Optical Interrogation of the Spontaneous Dynamics of Prefrontal Cortical Networks. [Internet] [Doctoral dissertation]. Brown University; 2015. [cited 2021 Feb 26]. Available from: https://repository.library.brown.edu/studio/item/bdr:419386/.

Council of Science Editors:

Blaeser AS. Optical Interrogation of the Spontaneous Dynamics of Prefrontal Cortical Networks. [Doctoral Dissertation]. Brown University; 2015. Available from: https://repository.library.brown.edu/studio/item/bdr:419386/

8. Taylor, Jackson Richard. Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications.

Degree: 2013, Wake Forest University

URL: http://hdl.handle.net/10339/38578

► CaVbeta subunits are traditionally considered constituents of CaV complexes (CaV1or2, CaVbeta;, and CaV alpha2/delta), where they localize at the plasma membrane and serve to regulate… (more)

Subjects/Keywords: calcium

…calcium channel ChIP �� …. ��Chromatin Immunoprecipitation DHPR… …Introduction Part 1: History of Cavβ as a Cav subunit. The β subunit of the voltage gated calcium… …x28;≈ 160 kDa) and γ (≈ 33 kDa) subunits of the voltage-sensitive calcium… …voltage gated”) into calcium influx (hence “calcium channel”). Calcium influx… …voltage-gated calcium channel (permeability, gating, pharmacological sensitivity, and…

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APA (6^th Edition):

Taylor, J. R. (2013). Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/38578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Taylor, Jackson Richard. “Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications.” 2013. Thesis, Wake Forest University. Accessed February 26, 2021. http://hdl.handle.net/10339/38578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Taylor, Jackson Richard. “Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications.” 2013. Web. 26 Feb 2021.

Vancouver:

Taylor JR. Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications. [Internet] [Thesis]. Wake Forest University; 2013. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10339/38578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Taylor JR. Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications. [Thesis]. Wake Forest University; 2013. Available from: http://hdl.handle.net/10339/38578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

9. Bandali, Elhaam, 1990-. The influence of dietary fat and intestinal pH on calcium bioaccessibility: an in vitro study.

Degree: MS, Nutritional Sciences, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51231/

► Human studies measuring true fractional calcium absorption have shown that dietary fat is a significant predictor of calcium absorption. In murine models, dietary fat also… (more)

▼ Human studies measuring true fractional calcium absorption have shown that dietary fat is a significant predictor of calcium absorption. In murine models, dietary fat also increases calcium absorption, but whether there is a differential effect with the type of fatty acid (FA) on intestinal calcium absorption is less clear. Compared to monounsaturated FAs (MUFAs), saturated FAs (SFAs) decrease lipid membrane fluidity and have a greater intestinal transit time, potentially prolonging their interaction with calcium in the gut. In addition, SFAs bind to calcium forming insoluble soaps, increasing fecal fat excretion, and reducing calcium availability for absorption. In addition, luminal factors such as a higher pH in the GI tract could influence calcium absorption such as with certain medications or achlorhydria. The TNO gastrointestinal model (TIM-1) replicates the physiological activities occurring in the lumen of the stomach, duodenum, jejunum, and ileum and is used to study biological events preceding nutrient absorption (i.e., bioaccessibility). In this study, we examined two high fat formulas (SFA or MUFA enriched) compared to low fat and controlled for calcium (500 mg) and other micronutrients during a 5-hr experiment. Calcium bioaccessibility (CaB) was greater for the high compared to low fat test meal in the jejunum (p = 0.001). In addition, CaB with the SFA alone was higher than either LFD or MUFA (p < 0.01). However, there was no interaction between diet and CaB in the ileum or ileal efflux. During high gastrointestinal pH, CaB was similar between diets in the jejunum and ileum, and there was a trend for increasing non-bioaccessible calcium over time (p = 0.058). Furthermore, CaB was 90% (p = 0.003), 91% (p = 0.036), and 94% (p = 0.001) lower in the jejunum, ileum, and ileal efflux, respectively at high pH compared to normal gastrointestinal conditions. These findings suggest a dramatically lower CaB under high pH conditions, having implications for a wide range of patients with gastrointestinal disorders. In addition, the higher Ca absorption associated with a high fat diet found previously, may be partially explained by an increased CaB. Advisors/Committee Members: Shapses, Sue (chair).

Subjects/Keywords: Calcium – Metabolism

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APA (6^th Edition):

Bandali, Elhaam, 1. (2016). The influence of dietary fat and intestinal pH on calcium bioaccessibility: an in vitro study. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51231/

Chicago Manual of Style (16^th Edition):

Bandali, Elhaam, 1990-. “The influence of dietary fat and intestinal pH on calcium bioaccessibility: an in vitro study.” 2016. Masters Thesis, Rutgers University. Accessed February 26, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51231/.

MLA Handbook (7^th Edition):

Bandali, Elhaam, 1990-. “The influence of dietary fat and intestinal pH on calcium bioaccessibility: an in vitro study.” 2016. Web. 26 Feb 2021.

Vancouver:

Bandali, Elhaam 1. The influence of dietary fat and intestinal pH on calcium bioaccessibility: an in vitro study. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Feb 26]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51231/.

Council of Science Editors:

Bandali, Elhaam 1. The influence of dietary fat and intestinal pH on calcium bioaccessibility: an in vitro study. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51231/

Rutgers University

10. Anstice, Jinnie, 1976-. A microfluidic investigation of calcium oxalate crystallization.

Degree: MS, Chemistry, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/56996/

► Calcium oxalate crystallization is widely studied due to the prevalence of this substance in various biomineralization processes, especially the formation of kidney stones. Bulk crystallization… (more)

Subjects/Keywords: Calcium oxalate

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APA (6^th Edition):

Anstice, Jinnie, 1. (2018). A microfluidic investigation of calcium oxalate crystallization. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/56996/

Chicago Manual of Style (16^th Edition):

Anstice, Jinnie, 1976-. “A microfluidic investigation of calcium oxalate crystallization.” 2018. Masters Thesis, Rutgers University. Accessed February 26, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/56996/.

MLA Handbook (7^th Edition):

Anstice, Jinnie, 1976-. “A microfluidic investigation of calcium oxalate crystallization.” 2018. Web. 26 Feb 2021.

Vancouver:

Anstice, Jinnie 1. A microfluidic investigation of calcium oxalate crystallization. [Internet] [Masters thesis]. Rutgers University; 2018. [cited 2021 Feb 26]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/56996/.

Council of Science Editors:

Anstice, Jinnie 1. A microfluidic investigation of calcium oxalate crystallization. [Masters Thesis]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/56996/

University of Manchester

11. Njegic, Alexandra Maria. Investigation of the function of PMCA1 in physiological and pathological angiogenesis.

Degree: 2019, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:322686

► Angiogenesis occurs under physiological and pathological conditions and the ability to manipulate vessel growth makes it a promising therapeutic target; however, pro-angiogenic therapies have failed… (more)

▼ Angiogenesis occurs under physiological and pathological conditions and the ability to manipulate vessel growth makes it a promising therapeutic target; however, pro-angiogenic therapies have failed to translate successfully into the clinic therefore more therapeutic options are required. One such novel target are the plasma membrane calcium ATPases (PMCAs), with PMCA4 shown to negatively regulate angiogenesis both in vitro and in vivo. The PMCAs (PMCA1-4) act to extrude Ca2+ from the cytosol into the extracellular space and can also mediate intracellular signalling. Given this role for PMCA4 in angiogenesis we hypothesised that PMCA1, which is also present in endothelial cells, may play a role in angiogenesis. In this study, the contribution of PMCA1 to physiological angiogenesis was assessed using both in vitro and in vivo methods. To determine if PMCA1 plays a role in angiogenesis in vitro, siRNA-mediated ATP2B1 knockdown was performed in human umbilical vein endothelial cells (HUVECs) which were then used to determine changes to endothelial cell biology and angiogenic characteristics. Knockdown of PMCA1 in HUVECs led to an increase in basal intracellular Ca2+, a reduction in cell viability and impaired VEGF-mediated tubule formation. Mechanistically, preliminary work identified changes to proteins involved in regulation of the eukaryotic cell cycle; loss of PMCA1 leads to a reduction in 5 out of 6 core components of the mini chromosome complex. In addition, to determine the effect of PMCA1 on angiogenesis in vivo, a novel mouse line was generated using the pan-endothelial transgene Tie2 Cre (PMCA1Tie2). These mice are viable and display no overt phenotype under basal conditions; however, the extent of PMCA1 knockdown in endothelial cells from PMCA1Tie2 mice was only 30%. Furthermore, when subject to surgical pressure-overload induced hypertrophy, PMCA1Tie2 mice show a similar extent of cardiac remodelling when compared to littermate controls but have increased levels of the pro-angiogenic protein RCAN1.4. Overall, PMCA1 is required for effective VEGF-mediated angiogenesis and endothelial cell viability in vitro and loss of PMCA1 leads to downregulation of cell cycle components. However, the understanding of the contribution of PMCA1 to physiological and pathological angiogenesis in vivo is still not conclusive; the partial endothelial knockout achieved in this study suggests that endothelial cells may require PMCA1 in order to function normally, as such, the complete ablation of endothelial PMCA1 may not be achievable in vivo. Advisors/Committee Members: SHIELS, HOLLY HA, Cartwright, Elizabeth, Shiels, Holly.

Subjects/Keywords: Angiogenesis; Plasma membrane calcium ATPase1; Hypertrophy; Calcium

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APA (6^th Edition):

Njegic, A. M. (2019). Investigation of the function of PMCA1 in physiological and pathological angiogenesis. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:322686

Chicago Manual of Style (16^th Edition):

Njegic, Alexandra Maria. “Investigation of the function of PMCA1 in physiological and pathological angiogenesis.” 2019. Doctoral Dissertation, University of Manchester. Accessed February 26, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:322686.

MLA Handbook (7^th Edition):

Njegic, Alexandra Maria. “Investigation of the function of PMCA1 in physiological and pathological angiogenesis.” 2019. Web. 26 Feb 2021.

Vancouver:

Njegic AM. Investigation of the function of PMCA1 in physiological and pathological angiogenesis. [Internet] [Doctoral dissertation]. University of Manchester; 2019. [cited 2021 Feb 26]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:322686.

Council of Science Editors:

Njegic AM. Investigation of the function of PMCA1 in physiological and pathological angiogenesis. [Doctoral Dissertation]. University of Manchester; 2019. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:322686

12. Kozaily, Jad. Structure et dynamique d'aluminosilicates de calcium fondus : Structure and dynamics of calcium aluminosilicate melts.

Degree: Docteur es, Sciences des matériaux, 2012, Université d'Orléans

URL: http://www.theses.fr/2012ORLE2003

►

L’étude des silicates fondus présente un intérêt dans divers domaines de recherche comme la géologie ou la fabrication des verres avec des applications technologiques importantes… (more)

▼

L’étude des silicates fondus présente un intérêt dans divers domaines de recherche comme la géologie ou la fabrication des verres avec des applications technologiques importantes telles que par exemple, le confinement des déchets nucléaires. Ces recherches demandent des informations fondamentales sur la structure et la dynamique de ces liquides au niveau microscopique mais l’acquisition des données est très souvent limitée par les températures de fusion élevées des composés étudiés. Notre travail s’est donc basé sur l’utilisation de techniques sans contact afin de s’affranchir de cette difficulté. Dans le cadre de cette thèse, nous nous sommes intéressés à l’étude des propriétés structurales et dynamiques de divers aluminosilicates de calcium (CAS) fondus. Pour cela, nous avons développé un dispositif utilisant la lévitation aérodynamique afin d’effectuer des expériences de diffusion quasi-élastique des neutrons. En combinant ces mesures avec la diffusion inélastique des rayons X, nous avons pu obtenir des résultats sur la dynamique microscopique des CAS à l’état liquide ainsi que dans le régime de surfusion. En particulier, nous avons pu déterminer l’évolution de la viscosité avec la température et les coefficients de diffusion cohérents. Nous avons pu aussi étudier l’évolution de la dynamique de ces verres en fonction de l’augmentation de la quantité de silice dans la composition. En parallèle de nos travaux sur la dynamique, nous avons aussi réalisé des expériences de diffraction de neutrons et de rayons X sur les mêmes compositions et températures afin d’examiner l’ordre atomique local et essayer de le corréler aux propriétés dynamiques observées.

Because of their interesting properties as glass-forming systems, molten silicates play an important role in the geology of the Earth’s crust and mantle and are also of industrial interest for nuclear waste treatment. Research in these areas requires fundamental information on the microscopic structure and dynamics of silicate melts, but such measurements are hampered by the very high melting points of these systems. By extending the technique of aerodynamic levitation to inelastic neutron scattering, and also making use of inelastic synchrotron x-ray scattering, we have obtained results on the microscopic dynamics of silicates both above the melting point and in the supercooled regime. In particular, we have determined the temperature evolution of the viscosity and diffusion coefficient of calcium aluminosilicates, and thereby quantified the decrease in fragility of this glass-forming system as a function of increasing silica content. In parallel with our dynamical studies, we have performed x-ray and neutron diffraction experiments on the same compositions and temperatures in order to examine the local chemical order pertinent to the observed dynamical properties.

Advisors/Committee Members: Hennet, Louis (thesis director).

Subjects/Keywords: Calcium aluminosilicates; Lévitation aérodynamique; Calcium aluminosilicates; Levitation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kozaily, J. (2012). Structure et dynamique d'aluminosilicates de calcium fondus : Structure and dynamics of calcium aluminosilicate melts. (Doctoral Dissertation). Université d'Orléans. Retrieved from http://www.theses.fr/2012ORLE2003

Chicago Manual of Style (16^th Edition):

Kozaily, Jad. “Structure et dynamique d'aluminosilicates de calcium fondus : Structure and dynamics of calcium aluminosilicate melts.” 2012. Doctoral Dissertation, Université d'Orléans. Accessed February 26, 2021. http://www.theses.fr/2012ORLE2003.

MLA Handbook (7^th Edition):

Kozaily, Jad. “Structure et dynamique d'aluminosilicates de calcium fondus : Structure and dynamics of calcium aluminosilicate melts.” 2012. Web. 26 Feb 2021.

Vancouver:

Kozaily J. Structure et dynamique d'aluminosilicates de calcium fondus : Structure and dynamics of calcium aluminosilicate melts. [Internet] [Doctoral dissertation]. Université d'Orléans; 2012. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2012ORLE2003.

Council of Science Editors:

Kozaily J. Structure et dynamique d'aluminosilicates de calcium fondus : Structure and dynamics of calcium aluminosilicate melts. [Doctoral Dissertation]. Université d'Orléans; 2012. Available from: http://www.theses.fr/2012ORLE2003

13. Torrente, Angelo. Rôle de la signalisation calcique dans la génération et régulation de l’activité pacemaker du cœur : Role of calcium handling in the genesis and regulation of heart pacemaker activity.

Degree: Docteur es, Physiologie, 2011, Université Montpellier I

URL: http://www.theses.fr/2011MON1T023

►

Les pathologies du nœud sino-atrial provoquent un dysfonctionnement intrinsèque de l'automaticité de ce tissu. La maladie, l'âge ou une anomalie génétique peuvent être la cause… (more)

▼

Les pathologies du nœud sino-atrial provoquent un dysfonctionnement intrinsèque de l'automaticité de ce tissu. La maladie, l'âge ou une anomalie génétique peuvent être la cause d'un dérèglement de la fréquence cardiaque associé à la bradycardie ou la dysfonction atrio-ventriculire. Sans intervention médicale cette pathologie peut conduire à l'arrêt cardiaque. Les pathologies touchant le sinus sont fréquentes, avec une occurrence accrue dans la population âgée. Ainsi, avec le vieillissement des populations occidentales il devient primordial de mieux comprendre les mécanismes générant l'activité automatique du cœur. Les nombreuses études déjà réalisées dans ce domaine ont conduit à une forte controverse entre deux modèles explicatifs des mécanismes pacemaker. Dans ce contexte, j'ai étudié au cours de cette thèse le rôle joué par les canaux calciques Cav1.3 dans la génération de l'activité pacemaker. Afin de distinguer et expliquer le rôle des différents canaux ioniques impliqués dans le mécanisme pacemaker nous avons utilisé une combinaison d'outils pharmacologiques et plusieurs souches de souris transgéniques dont les gènes impliqués dans l'activité pacemaker étaient inactivés ou leur fonction modifiée. Dans le but de constituer un nouveau cadre d'interprétation du mécanisme pacemaker, plusieurs approches expérimentales ont été utilisées. L'activité pacemaker a été étudié dans 3 systèmes au niveau de complexité croissant: des cellules sino-atriales isolées, des tissus sino-atriales entiers et des animaux vivants. Ces différentes approches ont permis d'obtenir des résultats probants et ouvrent la voie à une meilleure compréhension de l'automaticité. En conclusion de cette thèse, nous proposons un cadre intégratif des modèles préexistants, dans lequel les canaux de la membrane cytoplasmique et les libérations de calcium interagissent pour générer un mécanisme pacemaker complexe.

The “sick sinus syndrome” has been defined as an intrinsic dysfunction of the heart Sino-atrial node to perform its pacemaking function. Disease, ageing, or gene defects may cause sinus dysfunctions, ranging from rhythm disturbance to bradycardia, sinus pauses, sinus arrest, and arrhythmias. Without medical intervention these dysfunctions can result in heart block. Sick sinus syndrome is a common pathology, with an increased penetration in the elder population. In the light of the actual increase of elderly in western population, a better comprehension of the mechanism generating spontaneous automaticity appears fundamental. Several investigations have been already carried out in the field of automaticity, leading to a heated controversy between two models of the pacemaker mechanism. In the light this controversy, we investigated the role of Ca2+ channels in generating pacemaker activity. In order to discern and explain the roles of different ion channel actors already implicated in the complex pacemaker machinery we used a combination of pharmacological tools and a variety of transgenic mice. These mice present genetic inactivation or…

Advisors/Committee Members: Mangoni, Matteo (thesis director).

Subjects/Keywords: Cœur; Calcium; Pacemaker; Heart; Calcium; Pacemaker

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Torrente, A. (2011). Rôle de la signalisation calcique dans la génération et régulation de l’activité pacemaker du cœur : Role of calcium handling in the genesis and regulation of heart pacemaker activity. (Doctoral Dissertation). Université Montpellier I. Retrieved from http://www.theses.fr/2011MON1T023

Chicago Manual of Style (16^th Edition):

Torrente, Angelo. “Rôle de la signalisation calcique dans la génération et régulation de l’activité pacemaker du cœur : Role of calcium handling in the genesis and regulation of heart pacemaker activity.” 2011. Doctoral Dissertation, Université Montpellier I. Accessed February 26, 2021. http://www.theses.fr/2011MON1T023.

MLA Handbook (7^th Edition):

Torrente, Angelo. “Rôle de la signalisation calcique dans la génération et régulation de l’activité pacemaker du cœur : Role of calcium handling in the genesis and regulation of heart pacemaker activity.” 2011. Web. 26 Feb 2021.

Vancouver:

Torrente A. Rôle de la signalisation calcique dans la génération et régulation de l’activité pacemaker du cœur : Role of calcium handling in the genesis and regulation of heart pacemaker activity. [Internet] [Doctoral dissertation]. Université Montpellier I; 2011. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2011MON1T023.

Council of Science Editors:

Torrente A. Rôle de la signalisation calcique dans la génération et régulation de l’activité pacemaker du cœur : Role of calcium handling in the genesis and regulation of heart pacemaker activity. [Doctoral Dissertation]. Université Montpellier I; 2011. Available from: http://www.theses.fr/2011MON1T023

University of Adelaide

14. Ball, Christine June. Calcium channel distribution in the arterial vascular tree and its relation to function.

Degree: 2010, University of Adelaide

URL: http://hdl.handle.net/2440/63325

► Clinical evidence in microvascular disease suggests that T-type Ca⁺⁺ channel blockers (CCBs) have benefits over conventional L-type CCBs, however the basis for this remains largely… (more)

▼ Clinical evidence in microvascular disease suggests that T-type Ca⁺⁺ channel blockers (CCBs) have benefits over conventional L-type CCBs, however the basis for this remains largely unknown. The objective of this study was to examine vascular reactivity utilising both pharmacological and molecular techniques. This thesis is composed of three sections including (A) an Introduction, (B) Functional Vascular Studies and (C) Molecular Vascular Studies. Section A summarised fundamental principles of the vasculature including an outline of the vascular system, vascular physiology, vascular cell biology, regulation of cytosolic Ca⁺⁺ and vascular pathophysiology. Section B utilised isolated vessels and wire myography to determine the effect of pre-treatment with L-type CCBs (verapamil and nifedipine) and combined L- and T-type CCBs (mibefradil and efonidipine) on endothelin-1 (Et-1) and K⁺-mediated contractile responses in large (rat aorta) and small (rat mesenteric and human subcutaneous) vessels. All four CCBs inhibited both Et-1 and K⁺-mediated contractile responses to a similar extent in large rat vessels, however in rat microvessels the combined L- and T-channel blockers produced significantly greater inhibition of contraction than L-channel blockers alone. The significance of this differential T-channel effect in microvessels was further supported by: (1) demonstration of divergent CCB responses in human microvessels, (2) incremental inhibition of constrictor responses with a combined L- and T-CCB despite maximal L-channel blockade, (3) utilisation of structurally diverse CCBs with varied affinity for L- and T-channels, (6) use of pharmacodynamically and therapeutically appropriate CCB concentrations, (7) confirmation of contractile agonist independent responses, (8) consistent results even in the presence of an altered microvascular physiology in the form of chronic Et-1 activation and (9) exclusion of an endothelium-dependent mechanism. Section C utilised the molecular techniques of quantitative polymerase chain reaction (PCR) and ratiometric western blotting to examine the distribution of the pore-forming subunits Caᵥ1.2, Caᵥ3.1 and 3.2 in both large (rat aorta) and small (rat mesenteric) vessels. The PCR data was equivocal with no difference noted in the distribution of the L- and T-channels between large and small vessels. In contrast to this, quantitative western blot analysis revealed that while there is a similar distribution of the three subunits in the large vessel, there is a significantly increased expression of both T-channel pore-forming subunits in microvessels (Caᵥ3.1: 112 ± 38%*, Caᵥ3.2: 168 ± 48%* relative to L-channel expression, *p<0.05). Considered together these ‘functional’ and ‘structural’ studies indicate the important role of the Ca⁺⁺ T-channel in regulating contractile responses in the microvasculature and their therapeutic potential. Advisors/Committee Members: Beltrame, John Francis (advisor), Saint, David Albert (advisor), Wilson, David Peter Murray (advisor), School of Medicine (school).

Subjects/Keywords: calcium; vasculature; microvasculature; calcium channel blocker

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ball, C. J. (2010). Calcium channel distribution in the arterial vascular tree and its relation to function. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/63325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ball, Christine June. “Calcium channel distribution in the arterial vascular tree and its relation to function.” 2010. Thesis, University of Adelaide. Accessed February 26, 2021. http://hdl.handle.net/2440/63325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ball, Christine June. “Calcium channel distribution in the arterial vascular tree and its relation to function.” 2010. Web. 26 Feb 2021.

Vancouver:

Ball CJ. Calcium channel distribution in the arterial vascular tree and its relation to function. [Internet] [Thesis]. University of Adelaide; 2010. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2440/63325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ball CJ. Calcium channel distribution in the arterial vascular tree and its relation to function. [Thesis]. University of Adelaide; 2010. Available from: http://hdl.handle.net/2440/63325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Yin, Liheng. Impact of the catecholaminergic polymorphic ventricular tachycardia (CPVT) mutation RyR2R420Q in cell function : Impacte des mutations du RyR2 (R420Q) liées à la tachycardie ventriculaire polymorphe sur la fonciton du cardiomyocyte.

Degree: Docteur es, Physiologie, physiopathologie, 2020, université Paris-Saclay

URL: http://www.theses.fr/2020UPASS068

► La tachycardie ventriculaire polymorphe catécholergique (CPVT) est une arythmie génétique létale qui se manifeste par une syncope ou une mort subite chez les enfants et… (more)

▼ La tachycardie ventriculaire polymorphe catécholergique (CPVT) est une arythmie génétique létale qui se manifeste par une syncope ou une mort subite chez les enfants et les jeunes adultes dans des conditions de stress sans anomalie structurelle cardiaque évidente. Plusieurs mécanismes ont été proposés pour expliquer les altérations fonctionnelles sous-jacentes de la libération de Ca2+ dues aux mutations de RyR2 ou de ses protéines accessoires. Une nouvelle mutation CPVT située sur la partie N terminale de RyR2 a été identifiée dans une famille espagnole (RyR2R420Q). Ici, nous avons utilisé un modèle de souris KI exprimant le canal RyR2R420Q et des cardiomyocytes différenciés de cellules souches pluripotentes induites (hiPS-CM) générées à partir de deux patients frères (l'un avec mutation, l'autre sans mutation utilisé comme témoin). L’analyse des cardiomyocytes ventriculaires exprimant le RyR2R420Q humain et de souris étudiées par imagerie Ca2+confocale montre une augmentation des libérations de Ca2+spontanée durant la diastole (visualisé par les Sparks Ca2+), une libération fractionnelle plus élevée et une fréquence de vagues Ca2+ proarythmogènes augmentée après stimulation à l'isoprotérénol. L’analyse électrophysiologique, étudiée en enregistrant les potentiels d'action (AP) en utilisant les techniques de micro-électrodes sur les hiPSC-CM et de patch-clamp sur les cellules ventriculaires de souris KI, a montré des post-dépolarisations retardées dépendants du Ca2+ (DAD). L’amplitude des transitoires [Ca2+]i des cellules stimulées à 1 Hz étaient plus faible chez le groupe muté. La charge en Ca2+ du réticulum sarcoplasmique (SR), estimée par application rapide de caféine (10 mM), était aussi plus réduite dans les hiPS-CM exprimant RyR2R420Q, avant et après l'application ISO (1 μM). Cependant, le RyR2R420Q semble plus enclin à libérer du Ca2+, car le transitoire [Ca2+]i normalisé par la quantité de Ca2+ stockée dans le SR, la libération fractionnaire, était plus élevée dans les cellules mutées. Même si la charge Ca2+ du SR était plus petite dans les cellules RyR2R420Q, elles présentaient souvent un comportement pro-arythmogène tel que les vagues Ca2+ pendant les périodes diastoliques. Ce comportement est encore augmentée lors de la stimulation -adrénergique. Des résultats similaires ont été observés chez les souris KI, montrant ce modèle comme un outil précieux pour étudier la maladie CPVT. Nous avons ensuite étudié l'effet antiarythmique potentiel de la venlafaxine et de la prégabaline dans les cardiomyocytes de souris KI et le hiPS-CM, deux médicaments parmi d'autres prescrits à un membre porteur de la famille et qui a montré une réversion des symptômes du CPVT après le traitement. Nous avons constaté que ces deux médicaments atténuaient les événements arythmogènes de libération du Ca2+ induits par l'ISO dans les cardiomyocytes de souris KI. La venlafaxine a montré un effet antiarythmique dans hiPS-CM à la fois en traitements aigus et chroniques.Ainsi 1) le RyR2R420Q montre une augmentation de la libération… Advisors/Committee Members: Gómez García, Ana-María (thesis director), Benitah, Jean-Pierre (thesis director).

Subjects/Keywords: Arythmie; Calcium; RyR2; Arrhythmias; RyR2; Calcium

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yin, L. (2020). Impact of the catecholaminergic polymorphic ventricular tachycardia (CPVT) mutation RyR2R420Q in cell function : Impacte des mutations du RyR2 (R420Q) liées à la tachycardie ventriculaire polymorphe sur la fonciton du cardiomyocyte. (Doctoral Dissertation). université Paris-Saclay. Retrieved from http://www.theses.fr/2020UPASS068

Chicago Manual of Style (16^th Edition):

Yin, Liheng. “Impact of the catecholaminergic polymorphic ventricular tachycardia (CPVT) mutation RyR2R420Q in cell function : Impacte des mutations du RyR2 (R420Q) liées à la tachycardie ventriculaire polymorphe sur la fonciton du cardiomyocyte.” 2020. Doctoral Dissertation, université Paris-Saclay. Accessed February 26, 2021. http://www.theses.fr/2020UPASS068.

MLA Handbook (7^th Edition):

Yin, Liheng. “Impact of the catecholaminergic polymorphic ventricular tachycardia (CPVT) mutation RyR2R420Q in cell function : Impacte des mutations du RyR2 (R420Q) liées à la tachycardie ventriculaire polymorphe sur la fonciton du cardiomyocyte.” 2020. Web. 26 Feb 2021.

Vancouver:

Yin L. Impact of the catecholaminergic polymorphic ventricular tachycardia (CPVT) mutation RyR2R420Q in cell function : Impacte des mutations du RyR2 (R420Q) liées à la tachycardie ventriculaire polymorphe sur la fonciton du cardiomyocyte. [Internet] [Doctoral dissertation]. université Paris-Saclay; 2020. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2020UPASS068.

Council of Science Editors:

Yin L. Impact of the catecholaminergic polymorphic ventricular tachycardia (CPVT) mutation RyR2R420Q in cell function : Impacte des mutations du RyR2 (R420Q) liées à la tachycardie ventriculaire polymorphe sur la fonciton du cardiomyocyte. [Doctoral Dissertation]. université Paris-Saclay; 2020. Available from: http://www.theses.fr/2020UPASS068

University of Georgia

16. Sharma, Manan. Survival of salmonella in orange juice fortified with calcium.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/20135

► Outbreaks of salmonellosis in orange juice raised interest about the survival of Salmonella spp. in juice supplemented with calcium. Commercially calcium- fortified orange juice or… (more)

Subjects/Keywords: Salmonella; orange juice; calcium; fortification; calcium lactate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sharma, M. (2014). Survival of salmonella in orange juice fortified with calcium. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/20135

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sharma, Manan. “Survival of salmonella in orange juice fortified with calcium.” 2014. Thesis, University of Georgia. Accessed February 26, 2021. http://hdl.handle.net/10724/20135.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sharma, Manan. “Survival of salmonella in orange juice fortified with calcium.” 2014. Web. 26 Feb 2021.

Vancouver:

Sharma M. Survival of salmonella in orange juice fortified with calcium. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10724/20135.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sharma M. Survival of salmonella in orange juice fortified with calcium. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/20135

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

17. Njegic, Alexandra. Investigation of the function of PMCA1 in physiological and pathological angiogenesis.

Degree: PhD, 2019, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-the-function-of-pmca1-in-physiological-and-pathological-angiogenesis(815827f0-3e7a-4f85-9f84-1cd1e2e9d3d1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799352

► Angiogenesis occurs under physiological and pathological conditions and the ability to manipulate vessel growth makes it a promising therapeutic target; however, pro-angiogenic therapies have failed… (more)

▼ Angiogenesis occurs under physiological and pathological conditions and the ability to manipulate vessel growth makes it a promising therapeutic target; however, pro-angiogenic therapies have failed to translate successfully into the clinic therefore more therapeutic options are required. One such novel target are the plasma membrane calcium ATPases (PMCAs), with PMCA4 shown to negatively regulate angiogenesis both in vitro and in vivo. The PMCAs (PMCA1-4) act to extrude Ca2+ from the cytosol into the extracellular space and can also mediate intracellular signalling. Given this role for PMCA4 in angiogenesis we hypothesised that PMCA1, which is also present in endothelial cells, may play a role in angiogenesis. In this study, the contribution of PMCA1 to physiological angiogenesis was assessed using both in vitro and in vivo methods. To determine if PMCA1 plays a role in angiogenesis in vitro, siRNA-mediated ATP2B1 knockdown was performed in human umbilical vein endothelial cells (HUVECs) which were then used to determine changes to endothelial cell biology and angiogenic characteristics. Knockdown of PMCA1 in HUVECs led to an increase in basal intracellular Ca2+, a reduction in cell viability and impaired VEGF-mediated tubule formation. Mechanistically, preliminary work identified changes to proteins involved in regulation of the eukaryotic cell cycle; loss of PMCA1 leads to a reduction in 5 out of 6 core components of the mini chromosome complex. In addition, to determine the effect of PMCA1 on angiogenesis in vivo, a novel mouse line was generated using the pan-endothelial transgene Tie2 Cre (PMCA1Tie2). These mice are viable and display no overt phenotype under basal conditions; however, the extent of PMCA1 knockdown in endothelial cells from PMCA1Tie2 mice was only 30%. Furthermore, when subject to surgical pressure-overload induced hypertrophy, PMCA1Tie2 mice show a similar extent of cardiac remodelling when compared to littermate controls but have increased levels of the pro-angiogenic protein RCAN1.4. Overall, PMCA1 is required for effective VEGF-mediated angiogenesis and endothelial cell viability in vitro and loss of PMCA1 leads to downregulation of cell cycle components. However, the understanding of the contribution of PMCA1 to physiological and pathological angiogenesis in vivo is still not conclusive; the partial endothelial knockout achieved in this study suggests that endothelial cells may require PMCA1 in order to function normally, as such, the complete ablation of endothelial PMCA1 may not be achievable in vivo.

Subjects/Keywords: Hypertrophy; Calcium; Angiogenesis; Plasma membrane calcium ATPase1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Njegic, A. (2019). Investigation of the function of PMCA1 in physiological and pathological angiogenesis. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-the-function-of-pmca1-in-physiological-and-pathological-angiogenesis(815827f0-3e7a-4f85-9f84-1cd1e2e9d3d1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799352

Chicago Manual of Style (16^th Edition):

Njegic, Alexandra. “Investigation of the function of PMCA1 in physiological and pathological angiogenesis.” 2019. Doctoral Dissertation, University of Manchester. Accessed February 26, 2021. https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-the-function-of-pmca1-in-physiological-and-pathological-angiogenesis(815827f0-3e7a-4f85-9f84-1cd1e2e9d3d1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799352.

MLA Handbook (7^th Edition):

Njegic, Alexandra. “Investigation of the function of PMCA1 in physiological and pathological angiogenesis.” 2019. Web. 26 Feb 2021.

Vancouver:

Njegic A. Investigation of the function of PMCA1 in physiological and pathological angiogenesis. [Internet] [Doctoral dissertation]. University of Manchester; 2019. [cited 2021 Feb 26]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-the-function-of-pmca1-in-physiological-and-pathological-angiogenesis(815827f0-3e7a-4f85-9f84-1cd1e2e9d3d1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799352.

Council of Science Editors:

Njegic A. Investigation of the function of PMCA1 in physiological and pathological angiogenesis. [Doctoral Dissertation]. University of Manchester; 2019. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-the-function-of-pmca1-in-physiological-and-pathological-angiogenesis(815827f0-3e7a-4f85-9f84-1cd1e2e9d3d1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.799352

Macquarie University

18. Colas, Bruno. Structural constraints on the crystallisation of amorphous calcium carbonate.

Degree: 2017, Macquarie University

URL: http://hdl.handle.net/1959.14/1261526

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Empirical thesis.

"The Bragg Institute, Australian Nuclear Science and Technology Organisation ; Australian Research Council Centre of Excellence for Core to Crust Fluid Systems (CCFS)… (more)

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Empirical thesis.

"The Bragg Institute, Australian Nuclear Science and Technology Organisation ; Australian Research Council Centre of Excellence for Core to Crust Fluid Systems (CCFS) and GEMOC" – at foot of title.

Bibliography: pages 133-149.

1. Introduction – 2. Calcium Carbonate preparation and characterisation – 3. Kinetics of particle growth and crystallisation of calcium carbonate – 4. Determination of the atomic structure of ACC – 5. Conclusion – Appendices – List of symbols – Bibliograohy.

Amorphous calcium carbonate (ACC) is a biogenic precursor of calcium carbonates forming shells and skeletons of marine organisms which are key components of the whole marine environment. Understanding carbonate formation is an essential prerequisite to quantify the effect climate change and pollution have on marine population, to build biogenic climate proxy archives and to design novel functional materials by biomimetism.

Water is a critical component of the structure of ACC and a component controlling the stability of the amorphous phase. Addition of small amounts of magnesium (1–10% of the calcium content) is known to promote the stability of ACC, presumably through stabilization of the hydrogen bonding network. Understanding the hydrogen bonding network in ACC is fundamental to understand the stability of ACC.

I successfully developed a synthesis protocol to make stable Amorphous Calcium Carbonate.This study describes the sample synthesis and characterization methods. This is followed by a description of the nucleation and crystallisation of calcium carbonate, along a non-classical pathway. The results are parameterised in terms of kinetics and final product as a function of magnesium doping.

The approach used in this study to determine the atomic structure of ACC is to use Monte-Carlo simulations, using the Empirical Potential Structure Refinement program, constrained by X-ray and neutron scattering data. X-ray data are suitable for determining Ca, Mg and O correlations, and neutron data give information on the hydrogen / deuterium (as the interaction of X-rays with hydrogen is too low for us to be able to constrain hydrogen atom positions with only X-rays). The final atomic model includes water molecule positions.

This new approach is used to constrain current novel kinetic models, shed light on the role of impurities and provide a basis for understanding biogenic systems in general.

1 online resource (xx, 149 pages) illustrations (some colour)

Advisors/Committee Members: Macquarie University. Department of Earth and Planetary Sciences, Australian Nuclear Science and Technology Organisation, ARC Centre of Excellence for Core to Crust Fluid Systems (CCFS).

Subjects/Keywords: Calcium carbonate; Crystallization; amorphous calcium carbonate; crystallisation

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APA (6^th Edition):

Colas, B. (2017). Structural constraints on the crystallisation of amorphous calcium carbonate. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/1261526

Chicago Manual of Style (16^th Edition):

Colas, Bruno. “Structural constraints on the crystallisation of amorphous calcium carbonate.” 2017. Doctoral Dissertation, Macquarie University. Accessed February 26, 2021. http://hdl.handle.net/1959.14/1261526.

MLA Handbook (7^th Edition):

Colas, Bruno. “Structural constraints on the crystallisation of amorphous calcium carbonate.” 2017. Web. 26 Feb 2021.

Vancouver:

Colas B. Structural constraints on the crystallisation of amorphous calcium carbonate. [Internet] [Doctoral dissertation]. Macquarie University; 2017. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1959.14/1261526.

Council of Science Editors:

Colas B. Structural constraints on the crystallisation of amorphous calcium carbonate. [Doctoral Dissertation]. Macquarie University; 2017. Available from: http://hdl.handle.net/1959.14/1261526

19. Hamoudi, Amine. Réaction alcali-silice dans le béton : étude de la dégradation structurale comparée de composés SiO2 (silice amorphe, quartz, silex) : Alkali silica reaction in the concrete : study of the compared structural degradation of SiO2 compounds (silica glass, quartz, flint).

Degree: Docteur es, Molécules et matière condensée, 2009, Université Lille I – Sciences et Technologies

URL: http://www.theses.fr/2009LIL10113

►

Les liens qui unissent la structure et les propriétés physiques, chimiques des matériaux sont très importants. En particulier, le degré d’ordre, les défauts cristallins, l’hétérogénéité… (more)

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Les liens qui unissent la structure et les propriétés physiques, chimiques des matériaux sont très importants. En particulier, le degré d’ordre, les défauts cristallins, l’hétérogénéité structurale ou chimique, s’avèrent être des paramètres très pertinents dans la réactivité des matériaux. Ce travail a porté sur l’étude de la réactivité de plusieurs formes de la silice, et permet de montrer l’influence de l’ordre et de la cristallinité sur les processus de vieillissement par la réaction alcali-silice. Dans cette objectif, la réactivité de trois matériaux différents : granulats (silex), quartz et verre de silice a été suivie par différentes techniques d’investigations.Le quartz réagit très lentement, le verre très vite et le granulat de manière intermédiaire. Une analyse plus fine montre que la fraction mal cristallisée, voire amorphe, du granulat réagit préférentiellement.Le caractère désordonné des matériaux suggère l’utilisation de techniques sensibles à l’ordre local : nous avons employé, la spectroscopie d’absorption X (XANES et EXAFS) complétée par la RMN du solide.Les cartographies chimiques obtenues par microscopie électronique (en balayage et en transmission) ou au synchrotron montrent que le potassium K+ diffuse le premier et que le calcium Ca++ pénètre difficilement la structure cristalline de la silice. A l’échelle locale la RMN du silicium montre la dépolymérisation du réseau permet la diffusion des cations Ca++.Le micro-XANES révèle la présence de plusieurs environnements autour des atomes de silicium. L’un consiste en un silicium avec quatre oxygènes premiers voisins et l’autre avec moins de quatre atomes d’oxygènes. D’autre part, l’EXAFS montre l’absence du Ca et/ou K au niveau de la deuxième couche de voisins de l’atome de silicium absorbeur dans un environnement ordonné.

The relationships between the structure and the physical and chemical properties are very important in materials. In particular, the degree of order, the crystal defects and the structural heterogeneities or chemical parameters appear to play a key role in the reactivity of materials. This work is devoted to the study of the reactivity of silica-based materials, and shows of the influence of ordering and crystallinity on the sensitivity to the alkali silica reaction. In this perspective, the reactivity of three different materials: silica glass, aggregates (Flint) and quartz was followed by various techniques. Quartz reacts very slowly whereas the silica glass reacts very quickly, the aggregate exhibiting an intermediate behavior. A closer analysis shows that the amorphous or poorly crystalline fraction of the aggregate reacts preferentially. The role played by these poorly crystalline fractions suggests the use of techniques sensitive to short and medium range order such as: X-ray absorption spectroscopy (XANES and EXAFS) and solid state NMR. Chemical maps obtained by electron microscopy (scanning or in transmission) or at the synchrotron, show that the potassium K+ is the first to diffuse and that penetration of calcium Ca++ is…

Advisors/Committee Members: Khouchaf, Lahcen (thesis director), Cordier, Patrick (thesis director).

Subjects/Keywords: Silicates de calcium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hamoudi, A. (2009). Réaction alcali-silice dans le béton : étude de la dégradation structurale comparée de composés SiO2 (silice amorphe, quartz, silex) : Alkali silica reaction in the concrete : study of the compared structural degradation of SiO2 compounds (silica glass, quartz, flint). (Doctoral Dissertation). Université Lille I – Sciences et Technologies. Retrieved from http://www.theses.fr/2009LIL10113

Chicago Manual of Style (16^th Edition):

Hamoudi, Amine. “Réaction alcali-silice dans le béton : étude de la dégradation structurale comparée de composés SiO2 (silice amorphe, quartz, silex) : Alkali silica reaction in the concrete : study of the compared structural degradation of SiO2 compounds (silica glass, quartz, flint).” 2009. Doctoral Dissertation, Université Lille I – Sciences et Technologies. Accessed February 26, 2021. http://www.theses.fr/2009LIL10113.

MLA Handbook (7^th Edition):

Hamoudi, Amine. “Réaction alcali-silice dans le béton : étude de la dégradation structurale comparée de composés SiO2 (silice amorphe, quartz, silex) : Alkali silica reaction in the concrete : study of the compared structural degradation of SiO2 compounds (silica glass, quartz, flint).” 2009. Web. 26 Feb 2021.

Vancouver:

Hamoudi A. Réaction alcali-silice dans le béton : étude de la dégradation structurale comparée de composés SiO2 (silice amorphe, quartz, silex) : Alkali silica reaction in the concrete : study of the compared structural degradation of SiO2 compounds (silica glass, quartz, flint). [Internet] [Doctoral dissertation]. Université Lille I – Sciences et Technologies; 2009. [cited 2021 Feb 26]. Available from: http://www.theses.fr/2009LIL10113.

Council of Science Editors:

Hamoudi A. Réaction alcali-silice dans le béton : étude de la dégradation structurale comparée de composés SiO2 (silice amorphe, quartz, silex) : Alkali silica reaction in the concrete : study of the compared structural degradation of SiO2 compounds (silica glass, quartz, flint). [Doctoral Dissertation]. Université Lille I – Sciences et Technologies; 2009. Available from: http://www.theses.fr/2009LIL10113

Boston University

20. McLoed, Melissa. THE mitochondrial uniporter modulates neuronal regenerative outgrowth and calcium dynamics following axotomy in C. elegans.

Degree: 2015, Boston University

URL: http://hdl.handle.net/2144/13983

► Following neuronal injury, calcium signaling plays a critical role in promoting repair processes. Injury produces an initial cytosolic calcium elevation mediated by calcium entry from… (more)

Subjects/Keywords: Neurosciences; Calcium; Mitochondria

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APA (6^th Edition):

McLoed, M. (2015). THE mitochondrial uniporter modulates neuronal regenerative outgrowth and calcium dynamics following axotomy in C. elegans. (Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/13983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McLoed, Melissa. “THE mitochondrial uniporter modulates neuronal regenerative outgrowth and calcium dynamics following axotomy in C. elegans.” 2015. Thesis, Boston University. Accessed February 26, 2021. http://hdl.handle.net/2144/13983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McLoed, Melissa. “THE mitochondrial uniporter modulates neuronal regenerative outgrowth and calcium dynamics following axotomy in C. elegans.” 2015. Web. 26 Feb 2021.

Vancouver:

McLoed M. THE mitochondrial uniporter modulates neuronal regenerative outgrowth and calcium dynamics following axotomy in C. elegans. [Internet] [Thesis]. Boston University; 2015. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/2144/13983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McLoed M. THE mitochondrial uniporter modulates neuronal regenerative outgrowth and calcium dynamics following axotomy in C. elegans. [Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/13983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Macha, Innocent. Calcium phosphate and bioglass reinforced PLA thin film biocomposites for slow drug delivery applications .

Degree: 2016, University of Dar es Salaam

URL: http://hdl.handle.net/20.500.11810/2636

► The rise in the number of musculoskeletal disorders (MSDs) due to the increase in aging population and advancement in medical technology has led to an… (more)

▼ The rise in the number of musculoskeletal disorders (MSDs) due to the increase in aging population and advancement in medical technology has led to an increasing demand for medication to prevent and treat these diseases. The development of new drugs or formulations to allow treatment of these diseases in their very early stages is only increasing. Local direct and multidelivery of medication and key minerals to support bone repair and regeneration at the defect site, from flexible degradable devices at the rate within the therapeutic window, seems to be an effective strategy. However current drug delivery vehicles are neither flexible and degradable, nor able to deliver both medication and minerals effectively. Using a simple “solution casting” method, preparation of medical devices with such potential for slow drug delivery for biomedical applications served as the research objective. Polylactic acid (PLA) and hydroxyapatite-hydrothermally converted coral were used to develop PLA thin film composites as drug delivery systems. PLA provided flexibility and biodegradability of the systems, while coralline hydroxyapatite provided a unique architecture with its porous and bioactive nature, which is suitable for drug loading and slow drug release. Two drugs, gentamicin (antibiotic) and bisphosphonate were loaded into the device and their release profiles and activities were studied for the treatment of medical-implant related infection and osteoposis respectively. The biocompatibility study on human adipose derived stem cells (hADSC) and biofilm formation behaviour of both gram-negative (Pseudomonas aeruginosa) and gram-positive bacterial (Staphylococcus aureus) were studied on PLA thin film composites loaded with gentamicin. The mechanical properties of PLA-surface treated bioglass for tissue engineering applications was also studied. An alternative conversion method of coralline materials and other natural materials such as sea mussel and ostrich eggshells to calcium phosphate materials were also evaluated. Although nanosurface bioglass treated with 1% (3-Aminopropyl) triethoxysilane (APTES) suggested effective improvement in elongation at the break of PLA/bioglass composites, they lacked the required drug release efficiency. However, the PLA thin film composites displayed ability for potential applications in biomedical field as drug delivery systems. The flexibility they provide allows them to conform to any desired clinical shape and size. Incorporation of hydroxyapatite in the matrix, has the added advantages of controlled release, improved encapsulation efficiency, increased drug stability and maintenance of bioactivity and continuous supply of calcium Ca²⁺ and phosphate PO₄²⁻ ions, which can assist in bone regeneration and repair. Gentamicin release profiles, exhibited a steady state release rate, with significant antimicrobial activity even at high concentrations of bacteria. The systems also showed the potential for prolonged release of both antibiotic and bisphosphonate. The loading of the drug onto HAp…

Subjects/Keywords: Calcium phosphate; drugs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Macha, I. (2016). Calcium phosphate and bioglass reinforced PLA thin film biocomposites for slow drug delivery applications . (Doctoral Dissertation). University of Dar es Salaam. Retrieved from http://hdl.handle.net/20.500.11810/2636

Chicago Manual of Style (16^th Edition):

Macha, Innocent. “Calcium phosphate and bioglass reinforced PLA thin film biocomposites for slow drug delivery applications .” 2016. Doctoral Dissertation, University of Dar es Salaam. Accessed February 26, 2021. http://hdl.handle.net/20.500.11810/2636.

MLA Handbook (7^th Edition):

Macha, Innocent. “Calcium phosphate and bioglass reinforced PLA thin film biocomposites for slow drug delivery applications .” 2016. Web. 26 Feb 2021.

Vancouver:

Macha I. Calcium phosphate and bioglass reinforced PLA thin film biocomposites for slow drug delivery applications . [Internet] [Doctoral dissertation]. University of Dar es Salaam; 2016. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/20.500.11810/2636.

Council of Science Editors:

Macha I. Calcium phosphate and bioglass reinforced PLA thin film biocomposites for slow drug delivery applications . [Doctoral Dissertation]. University of Dar es Salaam; 2016. Available from: http://hdl.handle.net/20.500.11810/2636

University of Oxford

22. Ng, Siaw Wei. Calcium signalling in immune cells.

Degree: PhD, 2011, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:b3843e43-9503-4855-a280-35c0d28f65e6 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543039

► Inappropriate stimulation of mast cells can trigger allergies including asthma, allergic rhinitis and eczema which, combined, affect almost 30% of the population in western societies.… (more)

▼ Inappropriate stimulation of mast cells can trigger allergies including asthma, allergic rhinitis and eczema which, combined, affect almost 30% of the population in western societies. Mast cell activation begins with aggregation of IgE receptors in response to antigen. This then triggers a series of reactions resulting in the tyrosine phosphorylation of Syk kinase, PKC activation and ultimately both degranulation and secretion of leukotrienes and cytokines. CRAC channels are expressed on mast cells, and are essential for IgE-mediated mast cell activation. Previous work in our laboratory has shown that local Ca²⁺ influx through CRAC channels activates Ca2+-dependent phopholipase A₂, ERK and 5-lipoxygenase, resulting in LTC₄ secretion from mast cells. Therefore, I have investigated how Ca2+ microdomains through CRAC channels are detected and how they trigger cellular responses. I find that phosphorylation of Syk following antigen stimulation is enhanced by Ca²⁺ influx through CRAC channels. I also show synergy between CRAC channels and antigen in activating Syk. These findings reveal a novel positive feedback step in mast cell activation, where local Ca²⁺ entry through CRAC channels activates Syk which, in turn, supports CRAC channels. Earlier work from our group has demonstrated that in RBL cells, Ca2+ influx through CRAC channels induces expression of the gene c-fos, an important regulator of pro-inflammatory gene expression. I have discovered that local Ca²⁺ entry is sensed by the non-receptor tyrosine kinase Syk, which accumulates at the cell periphery. Syk then signals to the nucleus through recruitment of the transcription factor STAT5. The results therefore identify Syk as a new link in excitation-transcription coupling, converting local Ca²⁺ influx into expression of genes that are essential for immune cell activation. Activation of G protein-coupled cysteinyl leukotriene type I receptors by the pro-inflammatory molecule LTC₄ is tightly linked to immune cell function and the receptor is an established therapeutic target for allergies including asthma. Desensitization of cysteinyl leukotriene type I receptors arises following protein kinase C-dependent phosphorylation of three serine residues in the receptor C-terminus. Here I show that abolishing leukotriene receptor desensitization suppresses agonist-driven gene expression. Physiological concentrations of LTC₄ led to repetitive cytoplasmic Ca²⁺ oscillations, which were accompanied by the opening of store-operated CRAC channels in the plasma membrane. Ca²⁺ microdomains near the open channels were relayed to the nucleus to increase expression of the transcription factor c-fos. In the absence of receptor desensitization, agonist-driven gene expression was suppressed. Mechanistically, stimulation of non-desensitizing receptors evoked prolonged Ca²⁺ release, which led to accelerated Ca²⁺-dependent inactivation of CRAC channels and a subsequent loss of…

Subjects/Keywords: 616.079; Physiology; Calcium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ng, S. W. (2011). Calcium signalling in immune cells. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:b3843e43-9503-4855-a280-35c0d28f65e6 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543039

Chicago Manual of Style (16^th Edition):

Ng, Siaw Wei. “Calcium signalling in immune cells.” 2011. Doctoral Dissertation, University of Oxford. Accessed February 26, 2021. http://ora.ox.ac.uk/objects/uuid:b3843e43-9503-4855-a280-35c0d28f65e6 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543039.

MLA Handbook (7^th Edition):

Ng, Siaw Wei. “Calcium signalling in immune cells.” 2011. Web. 26 Feb 2021.

Vancouver:

Ng SW. Calcium signalling in immune cells. [Internet] [Doctoral dissertation]. University of Oxford; 2011. [cited 2021 Feb 26]. Available from: http://ora.ox.ac.uk/objects/uuid:b3843e43-9503-4855-a280-35c0d28f65e6 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543039.

Council of Science Editors:

Ng SW. Calcium signalling in immune cells. [Doctoral Dissertation]. University of Oxford; 2011. Available from: http://ora.ox.ac.uk/objects/uuid:b3843e43-9503-4855-a280-35c0d28f65e6 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543039

University of Manchester

23. Knight-Percival, Alexander Stephen. Low energy super-elastic scattering from laser excited calcium.

Degree: PhD, 2012, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/low-energy-superelastic-scattering-from-laser-excited-calcium(e37927e1-97d6-41eb-9a88-06109757c1a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558068

► Super-elastic scattering measurements were taken from calcium using the spectrometer described in this thesis. Calcium atoms were excited from the 41S0 ground state to the… (more)

▼ Super-elastic scattering measurements were taken from calcium using the spectrometer described in this thesis. Calcium atoms were excited from the 41S0 ground state to the 41P1 excited state using a high resolution continuous wave laser set to a wavelength of 423 nm. A beam of electrons with a well defined energy was directed at the laser excited calcium atoms. The excited state was then described by a set of atomic collision parameters P_lin, gamma and L_perp, found from measuring scattered electrons as a function of scattering angle and energy. The scattering chamber was held at a pressure of 3x10 -7 mbar. A resistively heated oven operating at 800 degrees celsius produced a well collimated calcium atomic beam containing the calcium atoms. The electron gun generated a beam of electrons of well defined momentum, whose energy could be changed from ~5 eV to over 100 eV. At energies less than 20 eV the rate of super-elastic electrons was very low, and so modifications were made to the spectrometer to automate data collection for long operating times without the need for user intervention. A new digitally controlled DC voltage supply was constructed to deliver the correct potentials to the electron-optical elements in the spectrometer. An internal microcontroller enabled supply voltages to be programmed either using the front panel or via an attached computer for automatic optimisation of spectrometer voltages using a simplex algorithm. New data was collected for the collision parameters over the full accessible angular range from 25 – 140 degrees, at energies of 8, 10 and 65 eV. The data at 8 and 10 eV was taken so as to resolve differences between theoretical models at low energies. Comparisons were made with a relativistic distorted wave calculation, an R-matrix calculation, an R-matrix calculation using B-splines and a convergent close coupling theory. A 2 eV discrepancy was identified in the measured electron energy which was thought to be due to stray fields in the chamber. With this considered, convergent close coupling predictions were found to be in excellent agreement with the experimental data.

Subjects/Keywords: 661; superelastic; calcium

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APA (6^th Edition):

Knight-Percival, A. S. (2012). Low energy super-elastic scattering from laser excited calcium. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/low-energy-superelastic-scattering-from-laser-excited-calcium(e37927e1-97d6-41eb-9a88-06109757c1a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558068

Chicago Manual of Style (16^th Edition):

Knight-Percival, Alexander Stephen. “Low energy super-elastic scattering from laser excited calcium.” 2012. Doctoral Dissertation, University of Manchester. Accessed February 26, 2021. https://www.research.manchester.ac.uk/portal/en/theses/low-energy-superelastic-scattering-from-laser-excited-calcium(e37927e1-97d6-41eb-9a88-06109757c1a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558068.

MLA Handbook (7^th Edition):

Knight-Percival, Alexander Stephen. “Low energy super-elastic scattering from laser excited calcium.” 2012. Web. 26 Feb 2021.

Vancouver:

Knight-Percival AS. Low energy super-elastic scattering from laser excited calcium. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2021 Feb 26]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/low-energy-superelastic-scattering-from-laser-excited-calcium(e37927e1-97d6-41eb-9a88-06109757c1a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558068.

Council of Science Editors:

Knight-Percival AS. Low energy super-elastic scattering from laser excited calcium. [Doctoral Dissertation]. University of Manchester; 2012. Available from: https://www.research.manchester.ac.uk/portal/en/theses/low-energy-superelastic-scattering-from-laser-excited-calcium(e37927e1-97d6-41eb-9a88-06109757c1a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558068

University of Minnesota

24. Visweswaran, Ramjay. The importance of calcium cycling and mitochondria in the local onset of alternans in the heart.

Degree: PhD, Biomedical Engineering, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/165107

► Action potential duration (APD) alternans can be accompanied by alternans in intracellular calcium, leading to electromechanical alternans. Electromechanical alternans is considered a substrate for ventricular… (more)

▼ Action potential duration (APD) alternans can be accompanied by alternans in intracellular calcium, leading to electromechanical alternans. Electromechanical alternans is considered a substrate for ventricular fibrillation, especially during pathophysiological conditions such as ischemia. The work in thesis seeks to elucidate the spatio-temporal evolution of alternans and to investigate the potential pathways through which they occur. High resolution mapping was used to simultaneously map membrane voltage and intracellular calcium in normal rabbit hearts. By mapping both parameters simultaneously in the same region of the heart, we were able to reveal that instability in calcium cycling plays a primary role in the development of EM alternans in the whole heart. Further, we were able to apply a special restitution portrait analysis to predict the onset of both calcium and APD alternans before it occurs. We also wanted to elucidate the mechanisms behind the increased incidences of arrhythmias during ischemia. By simulating ischemic and mitochondrial dysfunction in isolated rabbit hearts, we were able to show that mitochondrial stress caused by uncoupling of the mitochondria is responsible for early occurrence of both APD and calcium alternans in the heart, which in turn creates a substrate to ventricular arrhythmias. Thus, uncoupling of the mitochondrial network that occurs during ischemia might be the primary reason for increased incidences of arrhythmias in the heart during ischemia. Overall, this study improves our knowledge of alternans and their basic underlying mechanism which can be used in the development of better treatment and/or prevention strategies. Development of techniques to predict alternans before it occurs would be a valuable clinical tool, especially for use in implantable pacemakers paving the way for pre-emptive interventions. In addition, elucidating the mechanism or pathways of alternans formation would lead to targeted drug treatments to prevent alternans and thus, VF and sudden cardiac death.

Subjects/Keywords: Alternans; Calcium; Mitochondria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Visweswaran, R. (2014). The importance of calcium cycling and mitochondria in the local onset of alternans in the heart. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/165107

Chicago Manual of Style (16^th Edition):

Visweswaran, Ramjay. “The importance of calcium cycling and mitochondria in the local onset of alternans in the heart.” 2014. Doctoral Dissertation, University of Minnesota. Accessed February 26, 2021. http://hdl.handle.net/11299/165107.

MLA Handbook (7^th Edition):

Visweswaran, Ramjay. “The importance of calcium cycling and mitochondria in the local onset of alternans in the heart.” 2014. Web. 26 Feb 2021.

Vancouver:

Visweswaran R. The importance of calcium cycling and mitochondria in the local onset of alternans in the heart. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/11299/165107.

Council of Science Editors:

Visweswaran R. The importance of calcium cycling and mitochondria in the local onset of alternans in the heart. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/165107

Queens University

25. Jeziorski, Adam. Crustacean zooplankton sedimentary assemblages and the calcium concentration of softwater Ontario lakes .

Degree: Biology, 2011, Queens University

URL: http://hdl.handle.net/1974/6347

► In recent decades, many softwater lakes on the boreal shield have experienced significant reductions in aqueous calcium (Ca) concentrations. These declines are a long-term consequence… (more)

▼ In recent decades, many softwater lakes on the boreal shield have experienced significant reductions in aqueous calcium (Ca) concentrations. These declines are a long-term consequence of acid deposition due to the depletion of base cations from watershed soils. There is concern that in some lakes [Ca] may be falling to levels detrimental to the competitiveness of Ca-rich organisms. By examining the crustacean zooplankton remains preserved in lake sediments, this thesis provides field evidence of reduced [Ca] impacting a Ca-sensitive crustacean zooplankton species (Daphnia pulex). Additionally, a 770 lake data set compiled from several Ontario monitoring programs revealed that 62 % (an increase from 35% in the early 1980s) of the lakes were near or below the laboratory-determined Ca threshold (1.5 mg•L-1) for the growth and survival of D. pulex. To determine whether the 1.5 mg•L-1 Ca threshold could be observed in a spatial survey of crustacean zooplankton sedimentary remains, surface sediments from 36 softwater (Ca range 1-3 mg•L-1) Ontario lakes were analyzed. Significant differences in daphniid abundances across the Ca threshold were present; however, only for the D. longispina species complex, indicating differences in Ca tolerances within daphniid species complexes. Extending the analysis to a comparison of modern-day vs. preindustrial assemblages revealed that in the same 36 lakes there have been large declines (by up to 30%) in daphniid relative abundances since preindustrial times coincident with increases in Ca-poor species (i.e. Holopedium gibberum) irrespective of modern day pH. These findings demonstrate that in natural settings, the competitive disadvantages of Ca limitation may occur at a higher [Ca] than previously suspected. Finally, zooplankton sedimentary remains were analyzed from several “pristine” lakes in northwestern Ontario that have also experienced Ca declines in recent years. Reduced abundances of Ca-sensitive taxa and increases in Ca-insensitive fauna provided further evidence of the impacts of Ca decline independent of acid deposition. Collectively these analyses demonstrate the potential importance of Ca as an environmental stressor in softwater regions, as well as the need for further research in order to make better use of the available information preserved in the sediment record.

Subjects/Keywords: Cladocera ; Calcium decline

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APA (6^th Edition):

Jeziorski, A. (2011). Crustacean zooplankton sedimentary assemblages and the calcium concentration of softwater Ontario lakes . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jeziorski, Adam. “Crustacean zooplankton sedimentary assemblages and the calcium concentration of softwater Ontario lakes .” 2011. Thesis, Queens University. Accessed February 26, 2021. http://hdl.handle.net/1974/6347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jeziorski, Adam. “Crustacean zooplankton sedimentary assemblages and the calcium concentration of softwater Ontario lakes .” 2011. Web. 26 Feb 2021.

Vancouver:

Jeziorski A. Crustacean zooplankton sedimentary assemblages and the calcium concentration of softwater Ontario lakes . [Internet] [Thesis]. Queens University; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1974/6347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jeziorski A. Crustacean zooplankton sedimentary assemblages and the calcium concentration of softwater Ontario lakes . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

26. Thammakan, Nirawan. Synthesis and characterisation of calcium phosphate compositions by precipitation and 'biomimetic methods'.

Degree: PhD, 2016, University of Aberdeen

URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153374160005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.694692

► Calcium phosphate compounds have been widely utilised in the interdisciplinary field of biomaterials research and are used clinically in a number of medical devices. In… (more)

▼ Calcium phosphate compounds have been widely utilised in the interdisciplinary field of biomaterials research and are used clinically in a number of medical devices. In the present work, calcium phosphate compositions were prepared by two precipitation methods: a classic aqueous precipitation method and a novel biomimetic precipitation method. The aqueous precipitation method was used to produce a range of hydroxyapatite and carbonate-substituted hydroxyapatite compositions that would act as reference materials, but more importantly to allow a method of determining the Ca/P molar ratio of these compositions using X-ray diffraction data. The importance of this was that a simple technique would be required for studying the calcium phosphates produced later by biomimetic methods, as the very low yields prevents traditional methods such as XRF. It was found that Rietveld refinement of XRD data from precipitated apatites that had been heated at between 800 and 1200°C in air provided quantitative phase compositions that could then be used to calculate Ca/P molar ratios. In order to use synthesis conditions that were more similar to in vivo mineralisation, calcium phosphate compositions were directly prepared by using alkali-induced 'biomimetic' precipitation from Simulated Body Fluid (SBF) under controlled conditions. The key parameters that were studied were the effect of precipitation pH, the SBF concentration, the reaction time, the soaking time, and the magnesium and the carbonate ion concentration of the SBF solution. The key finding was that while the precipitated product obtained from 'conventional' SBF was an amorphous calcium phosphate with a Ca/P molar ratio of 1.5, irrespective of the pH, SBF concentration, reaction time or carbonate ion concentration, the gradual removal of magnesium ions from the SBF solution led to the precipitation of firstly a Ca-deficient apatite and eventually (for low Mg concentrations and a Mg-free SBF) a stoichiometric hydroxyapatite with a Ca/P molar ratio of 1.67. This method resulted in very low yields, typically (50 mg from 100 ml of SBF) but control of parameters could yield different phase compositions and could also affect the crystal size and the crystallinity of the product. These observations highlighting the important role of magnesium ions in the SBF on the composition of the calcium phosphate phase that would precipitate raised the question of what effect does SBF magnesium ion content have on the surface apatite precipitation on various biomaterials when incubated in SBF, which is the classic in vitro bioactivity test. Studies that incubated bioactive glass discs and powder (45S5 composition) and dense hydroxyapatite ceramic discs in conventional SBF and an Mg-free SBF showed that the rate of surface apatite precipitation was significantly affected by the Mg content in the SBF. For the case of bioactive glass discs, a surface apatite layer formed in 2 hours when incubated in an Mg-free SBF compared to 24 hours in conventional SBF.

Subjects/Keywords: 610.28; Calcium phosphate

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APA (6^th Edition):

Thammakan, N. (2016). Synthesis and characterisation of calcium phosphate compositions by precipitation and 'biomimetic methods'. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153374160005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.694692

Chicago Manual of Style (16^th Edition):

Thammakan, Nirawan. “Synthesis and characterisation of calcium phosphate compositions by precipitation and 'biomimetic methods'.” 2016. Doctoral Dissertation, University of Aberdeen. Accessed February 26, 2021. https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153374160005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.694692.

MLA Handbook (7^th Edition):

Thammakan, Nirawan. “Synthesis and characterisation of calcium phosphate compositions by precipitation and 'biomimetic methods'.” 2016. Web. 26 Feb 2021.

Vancouver:

Thammakan N. Synthesis and characterisation of calcium phosphate compositions by precipitation and 'biomimetic methods'. [Internet] [Doctoral dissertation]. University of Aberdeen; 2016. [cited 2021 Feb 26]. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153374160005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.694692.

Council of Science Editors:

Thammakan N. Synthesis and characterisation of calcium phosphate compositions by precipitation and 'biomimetic methods'. [Doctoral Dissertation]. University of Aberdeen; 2016. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153374160005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.694692

University of Toronto

27. Nahirny, Adrian. Elevated Intracellular Ca2+ Alters Mitochondrial Protein Import and the Accumulation of Intramitochondrial Proteins in Neurons.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29535

►

Most (99%) mitochondrial proteins are nuclear-encoded and must be imported into mitochondria. Deficits in mitochondrial protein import (MPI) affect mitochondrial function and can cause neurodegenerative… (more)

Subjects/Keywords: mitochondria; calcium; neuron; calcium; ionophore; calcium; A23187; degradation; 0719; 0317; 0307

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APA (6^th Edition):

Nahirny, A. (2011). Elevated Intracellular Ca2+ Alters Mitochondrial Protein Import and the Accumulation of Intramitochondrial Proteins in Neurons. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/29535

Chicago Manual of Style (16^th Edition):

Nahirny, Adrian. “Elevated Intracellular Ca2+ Alters Mitochondrial Protein Import and the Accumulation of Intramitochondrial Proteins in Neurons.” 2011. Masters Thesis, University of Toronto. Accessed February 26, 2021. http://hdl.handle.net/1807/29535.

MLA Handbook (7^th Edition):

Nahirny, Adrian. “Elevated Intracellular Ca2+ Alters Mitochondrial Protein Import and the Accumulation of Intramitochondrial Proteins in Neurons.” 2011. Web. 26 Feb 2021.

Vancouver:

Nahirny A. Elevated Intracellular Ca2+ Alters Mitochondrial Protein Import and the Accumulation of Intramitochondrial Proteins in Neurons. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/1807/29535.

Council of Science Editors:

Nahirny A. Elevated Intracellular Ca2+ Alters Mitochondrial Protein Import and the Accumulation of Intramitochondrial Proteins in Neurons. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29535

University of Minnesota

28. Zhang, Dan. The role of cellular calcium channels in planarian biology.

Degree: PhD, Pharmacology, 2011, University of Minnesota

URL: http://purl.umn.edu/144122

► Identification of signaling pathways and therein drugable targets, to manipulate stem cell behavior in vivo is a major focus of regenerative medicine. This dissertation focuses… (more)

▼ Identification of signaling pathways and therein drugable targets, to manipulate stem cell behavior in vivo is a major focus of regenerative medicine. This dissertation focuses on the role of Ca2+ channels in stem cell differentiation and regeneration in a simple in vivo model, the planarian flatworm. These animals maintain a totipotent population of stem cells that give rise to all the cell types in the worm. Previously, we discovered that the isoquinoline drug praziquantel (PZQ) caused a robust (100%) and complete duplication of the entire anterior-posterior (AP) axis during flatworm regeneration to yield two-headed (bipolar) organisms. My studies mechanistically dissect these observations to show that PZQ subverted regeneration via activation of a specific neuronal voltage-gated Ca2+ channel (VGCC) isoform (Cav1A). Surprisingly, another isoform Cav1B was found to play opposing roles in axis formation to promote tail regeneration, suggesting a delicate interplay between Ca2+ signals critical for nervous system regeneration. Further dissection of the downstream pathway showed that RNAi of Cav1A blocked PZQ-evoked bipolar regeneration, Ca2+ entry and decreases in Wnt levels, the output of Hedgehog signaling. Thus, these data demonstrated that calcium signaling regulated regeneration through modulating Hedgehog signaling, a pathway that has been shown to regulate neuronal stem cell behavior, patterning and growth in diverse development processes. Taken together, these findings add new insights into the mechanisms that govern planarian regeneration. Additionally, my work on intracellular Ca2+ release channels in this system led to the identification of the planarian inositol 1, 4, 5-trisphosphate receptor (IP3R). Studies designed to elucidate the biological significance of this protein by in vivo RNAi knockdown led to the discovery that sexual planarians underwent severe defects of laying eggs in the absence of IP3R, although it failed to produce an obvious phenotype in asexual worms. Thus, these data provided genetic evidence that IP3R plays an important role in regulating reproductive physiology in planarian flatworms. In summary, the data obtained in this thesis have revealed essential roles of Ca2+ signaling in regulating planarian stem cell differentiation and reproductive physiology.

Subjects/Keywords: Calcium channel; Calcium entry; Calcium signaling; Disease; Neurogenesis; Stem cell

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APA (6^th Edition):

Zhang, D. (2011). The role of cellular calcium channels in planarian biology. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/144122

Chicago Manual of Style (16^th Edition):

Zhang, Dan. “The role of cellular calcium channels in planarian biology.” 2011. Doctoral Dissertation, University of Minnesota. Accessed February 26, 2021. http://purl.umn.edu/144122.

MLA Handbook (7^th Edition):

Zhang, Dan. “The role of cellular calcium channels in planarian biology.” 2011. Web. 26 Feb 2021.

Vancouver:

Zhang D. The role of cellular calcium channels in planarian biology. [Internet] [Doctoral dissertation]. University of Minnesota; 2011. [cited 2021 Feb 26]. Available from: http://purl.umn.edu/144122.

Council of Science Editors:

Zhang D. The role of cellular calcium channels in planarian biology. [Doctoral Dissertation]. University of Minnesota; 2011. Available from: http://purl.umn.edu/144122

University of Otago

29. Roome, Christopher Joel. Cooperation of Calcium Ion Transport Mechanisms for Cerebellar Synapse Physiology .

Degree: 2011, University of Otago

URL: http://hdl.handle.net/10523/1809

► The cerebellum has often been referred to as the "neuronal machine", so called for the elegant architecture of neuronal circuitry and necessarily clockwork precision of… (more)

▼ The cerebellum has often been referred to as the "neuronal machine", so called for the elegant architecture of neuronal circuitry and necessarily clockwork precision of signal transmission therein (Eccles 1967; Ito 2006). To maintain such precision, many synapses within the cerebellar micro-circuitry are highly plastic and frequency dependant. In particular, short-term synaptic plasticity (STP) at an important excitatory synaptic pathway between cerebellar granule cells (CGCs) and Purkinje neurons (PN), called the "parallel fibre to Purkinje neuron synapse" (PF-PN), is an integral feature of the synapse and for cerebellar function as a whole (Dittman, Kreitzer et al. 2000; Boyden, Katoh et al. 2004). During transmission at PF-PN synapses, the dynamics of elevated pre-synaptic calcium (or residual calcium) dramatically impact the STP exhibited by the synapse (Zucker and Regehr 2002). Multiple cellular mechanisms function cooperatively to carefully control residual calcium dynamics and two principle pre-synaptic mechanisms expressed in CGCs include the sodium calcium exchanger (NCX) and plasma membrane calcium ATPase (PMCA) (Blaustein, Juhaszova et al. 2002; Ivannikov, Sugimori et al. 2010). Both PMCA and NCX proteins function to remove elevated intracellular calcium and their cooperative activity is thought to be critical for maintaining PF-PN synaptic behaviour (Regehr 1997; Empson, Garside et al. 2007). However, characterisation of NCX activity in pre-synaptic calcium control, its influence on synaptic transmission, and how it might interplay with PMCA activity at PF-PN synapses remains to be established. This research aimed to understand the cooperative activity of NCX and PMCA2 and its functional impact on PF-PN synaptic behaviour. To do this, calcium fluorescent imaging and electrophysiological recordings were made from the mouse cerebellum in vitro, whilst PMCA2 and NCX were sequentially removed by either pharmacological or genetic manipulation to assess their individual and combined activities. Two fluorescent calcium imaging techniques using a genetically encoded calcium indicator, GCaMP2 and a conventional calcium indicator, Calcium Green-1 Dextran, were utilised to determine the influence that cooperative NCX and PMCA2 activity has on PF pre-synaptic calcium dynamics. The impact that cooperative NCX and PMCA2 activity had on PF-PN synaptic behaviour was addressed using patch clamp electrophysiology to record PF-PN post-synaptic currents and assess STP of the synapse. To supplement experimental studies, a computational modelling approach was used throughout to aid interpretation of calcium fluorescent imaging experiments. The model simulated pre-synaptic calcium dynamics to provide a theoretical basis for how calcium efflux characteristics exhibited by NCX and PMCA2 dictate their cooperative interplay for pre-synaptic calcium control. This investigation has provided strong evidence for an important cooperative interplay of pre-synaptic NCX and PMCA2 activity, capable of influencing PF-PN synapse… Advisors/Committee Members: Empson, Ruth Mary (advisor).

Subjects/Keywords: presynaptic calcium; cerebellum; sodium calcium exchanger (NCX); plasma membrane calcium ATPase (PMCA)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roome, C. J. (2011). Cooperation of Calcium Ion Transport Mechanisms for Cerebellar Synapse Physiology . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1809

Chicago Manual of Style (16^th Edition):

Roome, Christopher Joel. “Cooperation of Calcium Ion Transport Mechanisms for Cerebellar Synapse Physiology .” 2011. Doctoral Dissertation, University of Otago. Accessed February 26, 2021. http://hdl.handle.net/10523/1809.

MLA Handbook (7^th Edition):

Roome, Christopher Joel. “Cooperation of Calcium Ion Transport Mechanisms for Cerebellar Synapse Physiology .” 2011. Web. 26 Feb 2021.

Vancouver:

Roome CJ. Cooperation of Calcium Ion Transport Mechanisms for Cerebellar Synapse Physiology . [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2021 Feb 26]. Available from: http://hdl.handle.net/10523/1809.

Council of Science Editors:

Roome CJ. Cooperation of Calcium Ion Transport Mechanisms for Cerebellar Synapse Physiology . [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1809

University of Utah

30. Pieper, Joel Robert. Development of a noninvasive calcium imaging probe.

Degree: MS, Bioengineering, 2013, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3459/rec/702

► Genetically encoded calcium indicators (GECIs) are Ca2+ sensitive fluorescent proteins that have expanded the usefulness of optical calcium imaging to longitudinal in vivo studies due… (more)

Subjects/Keywords: Calcium imaging; Calcium probe; Calmodulin; Genetically encoded calcium indicators; Infrared fluorescent protein

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APA (6^th Edition):

Pieper, J. R. (2013). Development of a noninvasive calcium imaging probe. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3459/rec/702

Chicago Manual of Style (16^th Edition):

Pieper, Joel Robert. “Development of a noninvasive calcium imaging probe.” 2013. Masters Thesis, University of Utah. Accessed February 26, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3459/rec/702.

MLA Handbook (7^th Edition):

Pieper, Joel Robert. “Development of a noninvasive calcium imaging probe.” 2013. Web. 26 Feb 2021.

Vancouver:

Pieper JR. Development of a noninvasive calcium imaging probe. [Internet] [Masters thesis]. University of Utah; 2013. [cited 2021 Feb 26]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3459/rec/702.

Council of Science Editors:

Pieper JR. Development of a noninvasive calcium imaging probe. [Masters Thesis]. University of Utah; 2013. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3459/rec/702

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