subject:(Calcium ions)

Columbia University

1. Brown, Genevieve Nicole. The Sustainment and Consequences of Cytosolic Calcium Signals in Osteocytes.

Degree: 2016, Columbia University

► Osteocytes are widely regarded as mechanosensors, capable of detecting changes in the mechanical environment of the bone tissue and modifying cellular responses accordingly. Indeed, an… (more)

▼ Osteocytes are widely regarded as mechanosensors, capable of detecting changes in the mechanical environment of the bone tissue and modifying cellular responses accordingly. Indeed, an intact osteocyte network is required for bone changes in response to unloading, and studies have shown that loading/unloading influences osteocyte expression of proteins that modulate bone turnover, such as sclerostin and receptor activator of nuclear factor kappa B ligand (RANKL). However, mechanisms underlying osteocyte mechanotransduction remain unclear. For instance, one of the earliest responses of bone cells to mechanical stimuli is a rise in intracellular, or cytosolic, calcium (Ca2+cyt), but the mechanisms by which osteocytes generate or utilize Ca2+ signals to direct bone adaptation are largely unknown. In this thesis, I explored the mechanisms underlying the sustainment of Ca2+cyt oscillations in osteocytes as well as downstream consequences of these patterns. I discovered that Ca2+cyt oscillations are generated in osteocytes by Ca2+ release from the endoplasmic reticulum and that the predominant expression of T-Type voltage sensitive Ca2+ channels in these cells facilitates this behavior. I also explored the role of the actin cytoskeleton – another prominent feature in osteocytes – and found that actin dynamics are important for the generation of Ca2+cyt signals. Furthermore, I confirmed that Ca2+cyt transients subsequently activate actomyosin contractions in osteocytes by monitoring interactions of osteocytes exposed to Ca2+ agonists on micropillar substrates. With this information, I sought to relate Ca2+cyt signaling and actomyosin contractility in osteocytes to their roles as coordinators of bone adaptation. Ca2+-dependent contractions have been shown to facilitate the release of extracellular vesicles, small membrane-enclosed packages of proteins that cells use for communication, in other cell types. I found that mechanical stimulation increased the production and release of extracellular vesicles in osteocytes, and this was dependent on Ca2+ signaling. These extracellular vesicles contained key bone regulatory proteins and were small enough to plausibly transport through the lacunocanalicular system. Thus, I uncovered a novel mechanotransduction pathway by which osteocytes may coordinate tissue-level adaptation. As an extension of this work, I also characterized these behaviors in new osteocyte cell lines which may better reflect native cell physiology. The work in this thesis anchors Ca2+ signaling as a critical osteocyte response to mechanical loading and adds to the body of work exploring how and why these signals are generated. The results of these studies add new information to the still limited knowledge of this important bone cell and extend Ca2+ signaling research by connecting early mechanosensation events to subsequent protein responses to mechanical loading. Understanding the mechanisms behind the robust Ca2+cyt oscillations in osteocytes and how they relate to their roles as coordinators…

Subjects/Keywords: Calcium ions; Intracellular calcium; Bone cells; Osteocytes; Biomedical engineering; Biology; Biomechanics

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APA (6^th Edition):

Brown, G. N. (2016). The Sustainment and Consequences of Cytosolic Calcium Signals in Osteocytes. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8Q52PXD

Chicago Manual of Style (16^th Edition):

Brown, Genevieve Nicole. “The Sustainment and Consequences of Cytosolic Calcium Signals in Osteocytes.” 2016. Doctoral Dissertation, Columbia University. Accessed January 25, 2021. https://doi.org/10.7916/D8Q52PXD.

MLA Handbook (7^th Edition):

Brown, Genevieve Nicole. “The Sustainment and Consequences of Cytosolic Calcium Signals in Osteocytes.” 2016. Web. 25 Jan 2021.

Vancouver:

Brown GN. The Sustainment and Consequences of Cytosolic Calcium Signals in Osteocytes. [Internet] [Doctoral dissertation]. Columbia University; 2016. [cited 2021 Jan 25]. Available from: https://doi.org/10.7916/D8Q52PXD.

Council of Science Editors:

Brown GN. The Sustainment and Consequences of Cytosolic Calcium Signals in Osteocytes. [Doctoral Dissertation]. Columbia University; 2016. Available from: https://doi.org/10.7916/D8Q52PXD

University of Oxford

2. Douglas, Sophie Georgina. Regulation of CRAC channels and agonist-induced Ca2+ signals.

Degree: PhD, 2012, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:ae94ca14-ac95-4ea6-b14a-14f9f7bafd63 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.581188

► Calcium ions (Ca2+) are extremely important intracellular messengers, activating a plethora of cellular processes. Growing evidence now points to a major role for the local… (more)

▼ Calcium ions (Ca2+) are extremely important intracellular messengers, activating a plethora of cellular processes. Growing evidence now points to a major role for the local Ca2+ signal in driving specific cellular responses. The simplest and most fundamental local Ca2+ signal is the Ca2+ microdomain, which rapidly forms when Ca2+ permeable ion channels open. In non-excitable cells the dominant Ca2+ entry channels are store-operated Ca2+ channels (SOCCs). The best characterised is the Ca2+ release activated Ca2+ (CRAC) channel. How local Ca2+ entry through CRAC channels impacts on channel function however is unclear. I have investigated the interaction between the Ca2+ binding protein calmodulin and CRAC channel activity and subsequent agonist-induced Ca2+ signals. Furthermore, I have investigated a role for mitofusin 2 (a protein that is known to tether the ER and mitochondria) on these Ca2+ signals. Using three different calmodulin mutant constructs with alterations to their Ca2+ binding sensitivities, I have shown that calmodulin facilitates CRAC channel dependent Ca2+ entry and maintains agonist-induced cytosolic Ca2+ oscillations in a lobe-specific manner. Calmodulin has four Ca2+ binding sites, two on the N-lobe and two on the C-lobe. I found a dominant negative calmodulin mutant (CAM4M, where all four binding sites had been mutated), or one where the C-lobe could not bind Ca2+ (CAM2C), impaired both Ca2+ influx through CRAC channels and maintenance of cytosolic Ca2+ oscillations. In contrast, a Ca2+-insensitive N-lobe mutant had little effect, (CAM2N). Knockdown of the mitochondrial Ca2+ uniporter regulator (MICU1) or mitochondrial membrane depolarization had similar effects to those seen with CAM4M or CAM2C, suggesting that at least in part, the action of calmodulin was through regulation of mitochondrial Ca2+ dynamics. This was confirmed by directly measuring the mitochondrial matrix Ca2+ concentration in intact RBL-1 cells using the mitochondrial targeted, fluorescent protein, pericam. Both CAM4M and disruption of mitochondrial Ca2+ buffering impaired agonist-induced mitochondrial Ca2+ uptake, suggesting that the modulation of CRAC channels occurred through Ca2+-calmodulin facilitation of mitochondrial Ca2+ uptake. Using a mutant Orai1 (A73E) that cannot bind calmodulin, I have shown that calmodulin tethered to the CRAC channel provides a major source of calmodulin for effective mitochondrial Ca2+ uptake. Physiological relevance of my proposed pathway was provided from experiments where I showed knockdown of MICU1 impaired agonist-induced CRAC channel dependent NFAT-1-driven gene expression. In addition, I establish a crucial role for mitochondrial MFN2 and presumably its ability to properly link the mitochondria and ER in the control of CRAC channels and agonist-induced Ca2+ signals.

Subjects/Keywords: 572.696; Physiology and anatomy; Calcium ions; Store-operated Calcium Channels

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APA (6^th Edition):

Douglas, S. G. (2012). Regulation of CRAC channels and agonist-induced Ca2+ signals. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:ae94ca14-ac95-4ea6-b14a-14f9f7bafd63 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.581188

Chicago Manual of Style (16^th Edition):

Douglas, Sophie Georgina. “Regulation of CRAC channels and agonist-induced Ca2+ signals.” 2012. Doctoral Dissertation, University of Oxford. Accessed January 25, 2021. http://ora.ox.ac.uk/objects/uuid:ae94ca14-ac95-4ea6-b14a-14f9f7bafd63 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.581188.

MLA Handbook (7^th Edition):

Douglas, Sophie Georgina. “Regulation of CRAC channels and agonist-induced Ca2+ signals.” 2012. Web. 25 Jan 2021.

Vancouver:

Douglas SG. Regulation of CRAC channels and agonist-induced Ca2+ signals. [Internet] [Doctoral dissertation]. University of Oxford; 2012. [cited 2021 Jan 25]. Available from: http://ora.ox.ac.uk/objects/uuid:ae94ca14-ac95-4ea6-b14a-14f9f7bafd63 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.581188.

Council of Science Editors:

Douglas SG. Regulation of CRAC channels and agonist-induced Ca2+ signals. [Doctoral Dissertation]. University of Oxford; 2012. Available from: http://ora.ox.ac.uk/objects/uuid:ae94ca14-ac95-4ea6-b14a-14f9f7bafd63 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.581188

Hong Kong University of Science and Technology

3. Leung, Ho Chi. Exploration of the contribution made by transmembrane ion fluxes to the regulation of neural induction in Xenopus spp.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-87529 ; https://doi.org/10.14711/thesis-b ; http://repository.ust.hk/ir/bitstream/1783.1-87529/1/th_redirect.html

► Transmembrane fluxes of Ca²⁺ and H⁺ are crucial for early development in vertebrates. In amphibians such as Xenopus laevis, transmembrane fluxes of Ca²⁺ and H⁺… (more)

Subjects/Keywords: Nerves ; Growth ; Regeneration ; Calcium ions ; Physiological transport ; Hydrogen ions ; Xenopus laevis ; Membranes (Biology)

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APA (6^th Edition):

Leung, H. C. (2016). Exploration of the contribution made by transmembrane ion fluxes to the regulation of neural induction in Xenopus spp. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-87529 ; https://doi.org/10.14711/thesis-b ; http://repository.ust.hk/ir/bitstream/1783.1-87529/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leung, Ho Chi. “Exploration of the contribution made by transmembrane ion fluxes to the regulation of neural induction in Xenopus spp.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed January 25, 2021. http://repository.ust.hk/ir/Record/1783.1-87529 ; https://doi.org/10.14711/thesis-b ; http://repository.ust.hk/ir/bitstream/1783.1-87529/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leung, Ho Chi. “Exploration of the contribution made by transmembrane ion fluxes to the regulation of neural induction in Xenopus spp.” 2016. Web. 25 Jan 2021.

Vancouver:

Leung HC. Exploration of the contribution made by transmembrane ion fluxes to the regulation of neural induction in Xenopus spp. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Jan 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-87529 ; https://doi.org/10.14711/thesis-b ; http://repository.ust.hk/ir/bitstream/1783.1-87529/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leung HC. Exploration of the contribution made by transmembrane ion fluxes to the regulation of neural induction in Xenopus spp. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-87529 ; https://doi.org/10.14711/thesis-b ; http://repository.ust.hk/ir/bitstream/1783.1-87529/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

4. Camiré, Olivier. Ca²+ mechanisms of synaptic integration and plasticity in inhibitory interneurons.

Degree: 2019, Université Laval

URL: http://hdl.handle.net/20.500.11794/37039

►

Tableau d'honneur de la FÉSP

La signalisation calcique dendritique joue un rôle important dans la régulation de mécanismes neuronaux, tels que la plasticité synaptique et… (more)

▼

Tableau d'honneur de la FÉSP

La signalisation calcique dendritique joue un rôle important dans la régulation de mécanismes neuronaux, tels que la plasticité synaptique et l’intégration de l’information transmise. Bien compris chez les neurones principaux, ce processus de régulation est moins étudié chez les divers types d’interneurones GABAergiques qui modulent l’acquisition et l’envoi de signaux neuronaux. Chez les interneurones à décharge rapide, un type d’interneurone commun dans les circuits corticaux, il a été démontré qu’il y a absence de rétropropagation des potentiels d’action dans les dendrites distales (Hu et al., 2010). Cette découverte a des implications fonctionnelles, car la rétropropagation des potentiels d’action est un signal important pour l’induction des formes de plasticité synaptique hebbiennes. Par contre, il a été suggéré que l’activité dendritique locale pourrait compenser pour l’absence de rétropropagation des potentiels d’action. En conséquence, ce travail porte sur l’étude des évènements calciques dans les dendrites distales des interneurones à décharge rapide. Nous avons cherché à déterminer s’il est possible de générer ces signaux calciques par stimulation dendritique locale, à étudier les mécanismes responsables de ces signaux et à déterminer si ces signaux jouent un rôle dans la régulation de la plasticité synaptique à ces synapses. Pour atteindre ces objectifs, nous avons utilisé une combinaison de méthodes électrophysiologiqes (patch-clamp en mode cellule entière), d’imagerie calcique deux-photons et de modélisation computationnelle. Nous avons pu établir qu’il est possible de générer des évènements calciques postsynaptiques supralinéaires dans les synapses excitatrices étudiées par stimulation électrique locale. Ces signaux sont médiés par l’influx calcique provenant de l’activation des récepteurs AMPA perméables au Ca2+, qui déclenche à son tour le relâchement de Ca2+ par les récepteurs ryanodine présents sur réserves calciques intracellulaires. Ces signaux comprennent aussi une contribution calcique mineure des récepteurs NMDA, et ils restent locaux (pas de propagation dans l’arbre dendritique). De plus, nous avons déterminé que ces évènements calciques supralinéaires produisent un revirement de la plasticité synaptique, car ils induisent la dépression à long-terme dans les synapses étudiées, alors que les signaux calciques de basse amplitude induisent la potentiation à long-terme. Nous avons aussi examiné si ces évènements calciques supralinéaires étaient générés de façon équivalente dans les dendrites apicales et basales, qui reçoivent des synapses de différentes sources. Nous avons observé que les signaux des dendrites apicales avaient une plus grande amplitude et étaient associés à un plus haut niveau de dépolarisation. À partir de la modélisation, nous avons pu prédire le nombre de synapses nécessaires à la génération de ces signaux et la contribution potentielle des mécanismes d’extrusion du Ca2+. Finalement, nous avons étudié la spécificité cellulaire des mécanismes…

Advisors/Committee Members: Topolnik, Lisa.

Subjects/Keywords: QU 7.5 UL 2019; Ions calcium; Plasticité neuronale; Interneurones; Synapses; Calcium – Métabolisme; Inhibition (Neurophysiologie)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Camiré, O. (2019). Ca²+ mechanisms of synaptic integration and plasticity in inhibitory interneurons. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/37039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Camiré, Olivier. “Ca²+ mechanisms of synaptic integration and plasticity in inhibitory interneurons.” 2019. Thesis, Université Laval. Accessed January 25, 2021. http://hdl.handle.net/20.500.11794/37039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Camiré, Olivier. “Ca²+ mechanisms of synaptic integration and plasticity in inhibitory interneurons.” 2019. Web. 25 Jan 2021.

Vancouver:

Camiré O. Ca²+ mechanisms of synaptic integration and plasticity in inhibitory interneurons. [Internet] [Thesis]. Université Laval; 2019. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/20.500.11794/37039.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Camiré O. Ca²+ mechanisms of synaptic integration and plasticity in inhibitory interneurons. [Thesis]. Université Laval; 2019. Available from: http://hdl.handle.net/20.500.11794/37039

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

5. Campi, Andrea Elyse. Mechanosensitive Ca2+ Signaling of Ex Vivo Osteocytes in Aging and Treatment.

Degree: 2019, Columbia University

URL: https://doi.org/10.7916/d8-v47n-py06

► Of the three major cell types in bone, osteocytes are considered the major mechanosensors, capable of detecting whole-bone mechanical forces at a cellular level and… (more)

▼ Of the three major cell types in bone, osteocytes are considered the major mechanosensors, capable of detecting whole-bone mechanical forces at a cellular level and coordinating tissue-level bone formation and resorption responses. The pathology of age-induced bone loss, a major factor in the development of osteoporosis, is attributed to impaired osteocyte mechanosensing. However, real-time evidence of immediate osteocyte responses to mechanical load to support the blunted tissue-level responses that have been demonstrated thus far is lacking. A ubiquitous cellular response upstream of many functions in all cell types, intracellular calcium (Ca2+) is an early mechanosensitive signal in osteocytes, wherein the response characteristics studied in systems of multiple scales are related to mechanical stimuli. Thus, this phenomenon can be characterized as a real-time measure of osteocyte mechanosensitivity. The objective of this thesis was to utilize an ex vivo model of osteocyte Ca2+ signaling to investigate potentially altered mechanosensitivity of the osteocyte network in two clinical contexts: aging, and a recently-approved therapy for treatment of osteoporosis. Additionally, we aimed to enhance this ex vivo model to identify a functional consequence of this robust Ca2+ signaling response to mechanical load in the context of osteocyte mechanotransduction. We first sought to characterize and compare Ca2+ signaling responses to mechanical load in osteocytes from aged and young-adult mice using an ex vivo model to visualize cell networks in viable mouse tibiae. We found that fewer osteocytes responded to whole-bone cyclic mechanical loading in aged mice tibiae compared to those from young-adult mice and did so in a delayed manner, suggesting a diminished mechanosensitivity to load. Osteocytes from aged mice also lacked the well-correlated relationship between Ca2+ signaling synchrony and cell-cell distance exhibited by young-adult osteocyte networks. Taken together, we have demonstrated, for the first time, a real-time measure of the dampened mechanosensing and lack of signal coordination in aged osteocyte networks in situ, which may contribute to blunted long-term bone formation responses to load. Next, we utilized the ex vivo Ca2+ signaling model to investigate the effect of bone formation in response to treatment with sclerostin antibody (Scl-Ab) on osteocyte mechanosensing. Previous studies have identified two phases of bone formation response to Scl-Ab treatment: an initial period of rapid bone formation with short-term dosing and a return to a steady phase of bone formation response with long-term dosing. Thus, we treated mice according to three groups: vehicle, short-term Scl-Ab, and long-term Scl-Ab. Serum P1NP assays and biweekly micro-CT scans throughout the treatment period confirmed the two phases of bone formation response to Scl-Ab. At the conclusion of treatment, under ex vivo whole-bone loading matched at 10 N, there were no significant differences in osteocyte Ca2+ signaling parameters between…

Subjects/Keywords: Biomedical engineering; Osteocytes; Calcium ions; Osteoporosis; Aging – Physiological aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Campi, A. E. (2019). Mechanosensitive Ca2+ Signaling of Ex Vivo Osteocytes in Aging and Treatment. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/d8-v47n-py06

Chicago Manual of Style (16^th Edition):

Campi, Andrea Elyse. “Mechanosensitive Ca2+ Signaling of Ex Vivo Osteocytes in Aging and Treatment.” 2019. Doctoral Dissertation, Columbia University. Accessed January 25, 2021. https://doi.org/10.7916/d8-v47n-py06.

MLA Handbook (7^th Edition):

Campi, Andrea Elyse. “Mechanosensitive Ca2+ Signaling of Ex Vivo Osteocytes in Aging and Treatment.” 2019. Web. 25 Jan 2021.

Vancouver:

Campi AE. Mechanosensitive Ca2+ Signaling of Ex Vivo Osteocytes in Aging and Treatment. [Internet] [Doctoral dissertation]. Columbia University; 2019. [cited 2021 Jan 25]. Available from: https://doi.org/10.7916/d8-v47n-py06.

Council of Science Editors:

Campi AE. Mechanosensitive Ca2+ Signaling of Ex Vivo Osteocytes in Aging and Treatment. [Doctoral Dissertation]. Columbia University; 2019. Available from: https://doi.org/10.7916/d8-v47n-py06

Hong Kong University of Science and Technology

6. Zhou, Lingyu. Development of novel tools to investigate Ca²⁺ signaling during the formation of the zebrafish heart.

Degree: 2014, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-87484 ; https://doi.org/10.14711/thesis-b1334204 ; http://repository.ust.hk/ir/bitstream/1783.1-87484/1/th_redirect.html

► Ca²⁺ signaling has been extensively studied in the mature heart. In addition to excitation-contraction coupling, Ca²⁺ also plays a major role in regulating heart development… (more)

Subjects/Keywords: Cellular signal transduction ; Calcium ions ; Heart ; Zebra danio ; Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, L. (2014). Development of novel tools to investigate Ca²⁺ signaling during the formation of the zebrafish heart. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-87484 ; https://doi.org/10.14711/thesis-b1334204 ; http://repository.ust.hk/ir/bitstream/1783.1-87484/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Lingyu. “Development of novel tools to investigate Ca²⁺ signaling during the formation of the zebrafish heart.” 2014. Thesis, Hong Kong University of Science and Technology. Accessed January 25, 2021. http://repository.ust.hk/ir/Record/1783.1-87484 ; https://doi.org/10.14711/thesis-b1334204 ; http://repository.ust.hk/ir/bitstream/1783.1-87484/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Lingyu. “Development of novel tools to investigate Ca²⁺ signaling during the formation of the zebrafish heart.” 2014. Web. 25 Jan 2021.

Vancouver:

Zhou L. Development of novel tools to investigate Ca²⁺ signaling during the formation of the zebrafish heart. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2014. [cited 2021 Jan 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-87484 ; https://doi.org/10.14711/thesis-b1334204 ; http://repository.ust.hk/ir/bitstream/1783.1-87484/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou L. Development of novel tools to investigate Ca²⁺ signaling during the formation of the zebrafish heart. [Thesis]. Hong Kong University of Science and Technology; 2014. Available from: http://repository.ust.hk/ir/Record/1783.1-87484 ; https://doi.org/10.14711/thesis-b1334204 ; http://repository.ust.hk/ir/bitstream/1783.1-87484/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

7. Chan, Yin Seng Harvey LIFS. Morphometric analysis and characterization of Ca²⁺ signaling in human embryonic stem cell-derived cardiomyocyte progenitors.

Degree: 2015, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-90274 ; https://doi.org/10.14711/thesis-b1477548 ; http://repository.ust.hk/ir/bitstream/1783.1-90274/1/th_redirect.html

► Quantitative methods were established to measure changes in the gross morphology (i.e., shape and area), cytoskeletal organization, and intercalation of mitochondria between the myofibrils in… (more)

▼ Quantitative methods were established to measure changes in the gross morphology (i.e., shape and area), cytoskeletal organization, and intercalation of mitochondria between the myofibrils in human embryonic stem cell-ventricular cardiomyocytes (hESC-vCMs) that had been treated with different metabolic stimulants (i.e., oleic acid and isoproterenol (ISO)) or electrical stimulation during early differentiation. Cells were double-immunolabelled with α-actinin and mitochondria (COX IV) antibodies, after which images were acquired via confocal microscopy. Image analysis protocols were then designed using Image J software to measure the extent of differentiation of cells within the population. My data demonstrated that treatment with oleic acid or ISO alone or with a combination of the two induced a more elongated phenotype of hESC-vCMs than the untreated controls. In addition, cells treated with oleic acid alone or oleic acid and ISO exhibited a more organized (parallel) orientation of myofibrils, and the combined treatment also resulted in enhanced intercalation of mitochondria between the myofibrils. These data suggest that when used alone, these metabolic stimulants have a variable effect on hESC-vCMs but when they are applied together their effect on inducing a more mature phenotype in these cells is enhanced. The possible role of endogenous Ca²⁺ signaling during human embryonic stem cell (hESC) differentiation and maturation into ventricular cardiomyocytes (vCMs) was investigated using hES2 cells that were transduced with the jellyfish apo-aequorin gene using a lentiviral vector. Active holo-aequorin was reconstituted in the apo-aequorin expressed cells, by incubation with f-coelenterazine, the prosthetic co-factor of the holo-aequorin complex. Holo-aequorin is a Ca²⁺-sensitive bioluminescent complex that emits light at ~470 nm on binding with Ca²⁺. The temporal nature of the Ca²⁺ signals generated by aggregates of these cells (called cardiospheres) at the earliest stages of differentiation into hESC-vCMs was then investigated using a luminometer. My data indicated that during the earliest stages of vCM differentiation, no endogenous Ca²⁺ signals could be detected using the current aequorin method. However, as vCMs matured both IP₃Rs and RyRs were shown to be expressed via Ca²⁺ transients generated after the addition of exogenous ATP and caffeine, respective agonists of these SR-located Ca²⁺ release channels.

Subjects/Keywords: Embryonic stem cells ; Heart cells ; Cellular signal transduction ; Calcium ions

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chan, Y. S. H. L. (2015). Morphometric analysis and characterization of Ca²⁺ signaling in human embryonic stem cell-derived cardiomyocyte progenitors. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-90274 ; https://doi.org/10.14711/thesis-b1477548 ; http://repository.ust.hk/ir/bitstream/1783.1-90274/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chan, Yin Seng Harvey LIFS. “Morphometric analysis and characterization of Ca²⁺ signaling in human embryonic stem cell-derived cardiomyocyte progenitors.” 2015. Thesis, Hong Kong University of Science and Technology. Accessed January 25, 2021. http://repository.ust.hk/ir/Record/1783.1-90274 ; https://doi.org/10.14711/thesis-b1477548 ; http://repository.ust.hk/ir/bitstream/1783.1-90274/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chan, Yin Seng Harvey LIFS. “Morphometric analysis and characterization of Ca²⁺ signaling in human embryonic stem cell-derived cardiomyocyte progenitors.” 2015. Web. 25 Jan 2021.

Vancouver:

Chan YSHL. Morphometric analysis and characterization of Ca²⁺ signaling in human embryonic stem cell-derived cardiomyocyte progenitors. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2015. [cited 2021 Jan 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-90274 ; https://doi.org/10.14711/thesis-b1477548 ; http://repository.ust.hk/ir/bitstream/1783.1-90274/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chan YSHL. Morphometric analysis and characterization of Ca²⁺ signaling in human embryonic stem cell-derived cardiomyocyte progenitors. [Thesis]. Hong Kong University of Science and Technology; 2015. Available from: http://repository.ust.hk/ir/Record/1783.1-90274 ; https://doi.org/10.14711/thesis-b1477548 ; http://repository.ust.hk/ir/bitstream/1783.1-90274/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

8. Rose, Allan J. Composition and gelling properties of whey protein concentrates in model gel and frankfurter systems as influenced by ionized calcium.

Degree: MS, Department of Food Science and Human Nutrition, 1993, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:24177

Subjects/Keywords: Whey; Colloids; Calcium ions

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APA (6^th Edition):

Rose, A. J. (1993). Composition and gelling properties of whey protein concentrates in model gel and frankfurter systems as influenced by ionized calcium. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:24177

Chicago Manual of Style (16^th Edition):

Rose, Allan J. “Composition and gelling properties of whey protein concentrates in model gel and frankfurter systems as influenced by ionized calcium.” 1993. Masters Thesis, Michigan State University. Accessed January 25, 2021. http://etd.lib.msu.edu/islandora/object/etd:24177.

MLA Handbook (7^th Edition):

Rose, Allan J. “Composition and gelling properties of whey protein concentrates in model gel and frankfurter systems as influenced by ionized calcium.” 1993. Web. 25 Jan 2021.

Vancouver:

Rose AJ. Composition and gelling properties of whey protein concentrates in model gel and frankfurter systems as influenced by ionized calcium. [Internet] [Masters thesis]. Michigan State University; 1993. [cited 2021 Jan 25]. Available from: http://etd.lib.msu.edu/islandora/object/etd:24177.

Council of Science Editors:

Rose AJ. Composition and gelling properties of whey protein concentrates in model gel and frankfurter systems as influenced by ionized calcium. [Masters Thesis]. Michigan State University; 1993. Available from: http://etd.lib.msu.edu/islandora/object/etd:24177

Université Paris-Sud – Paris XI

9. Jakutyte, Lina. Bacteriophage SPP1 entry into the host cell : Entrée de bactériophage SPP1 dans la cellule hôte.

Degree: Docteur es, Structure, fonction et ingénierie des protéines, 2011, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2011PA114842

► Les quatre étapes principales d'infection des bactéries par leurs virus sont (i) la reconnaissance spécifique de la cellule hôte et l'entrée du génome dans le… (more)

▼ Les quatre étapes principales d'infection des bactéries par leurs virus sont (i) la reconnaissance spécifique de la cellule hôte et l'entrée du génome dans le cytoplasme,(ii) la réplication du génome viral, (iii) l'assemblage des particules virales, et (iv) leur relâchement, menant dans la plupart des cas à la lyse de la cellule. Bien que la description des étapes individuelles du cycle viral a été relativement bien établie, les détails de comment d'ADN viral chemine du virion jusqu’au cytoplasme de la bactérie hôte et de comment l'environnement cellulaire participe au processus restent mal compris.La première étape de l’infection est la reconnaissance d’un récepteur à la surface de la bactérie hôte par la machinerie d’adsorption du phage. Les barrières que l’agent infectieux doit franchir par la suite sont la membrane externe de la bactérie Gram-negative, la paroi cellulaire et la membrane cytoplasmique. Ceci implique une dégradation localisée de la paroi et le cheminement de l’ADN à travers un pore dans la membrane. L‘ADN linéaire se circularise normalement dans le cytoplasme et il est répliqué par la suite. On a utilisé le bactériophage SPP1 qui infecte la bactérie Gram-positive Bacillus subtilis comme modèle d’étude pour disséquer ces différentes étapes clés pour le démarrage de l’infection virale. Dans ce travail de thèse les conditions d’infection et d’acquisition de données pour suivre en temps réel la dépolarisation de la membrane cellulaire de B. subtilis lors de l’infection par SPP1 ont été mis au point. Il est montré que le démarrage de l’infection déclenche une dépolarisation très rapide de la membrane cytoplasmique.Le potentiel de membrane n’est plus rétablit pendant toute la durée du cycle d'infection. Ce changement du potentiel de membrane au début de l’infection dépend de la présence du récepteur YueB. L’amplitude de la dépolarisation dépend du nombre de particules virales infectieuses présentes et de la concentration du récepteur YueB à la surface de la bactérie hôte. L’interaction du phage avec le récepteur YueB conduit à l’interaction irréversible et à l'éjection de l’ADN de SPP1. Pour établir si c’est l’interaction avec YueB ou le début de l’entrée de l’ADN qui conduit à la dépolarisation de la membrane on a utilisé des phages SPP1 éclates par EDTA qui adsorbent normalement à B. subtilis mais qui n’avaient plus leur ADN. Les résultats obtenus ont montré que la dépolarisation requiert l’interaction du virus intacte avec le récepteur YueB. Des concentrations sous-millimolaire de Ca2+ sont nécessaires et suffisantes pour SPP1 liaison réversible à l'enveloppe d'hôte et donc de déclencher la dépolarisation.La cinétique d’entrée de l’ADN du bactériophage SPP1 dans la bactérie Bacillus subtilis a été suivie en temps réel par microscopie de fluorescence. On a mis au point une méthode de microscopie pour visualiser des particules virales marquées avec des «quantum dots» ce qui permit de démontrer que ces particules se fixent préférentiellement aux pôles des bacilli. L’immuno-marquage du récepteur de… Advisors/Committee Members: Tavares, Paulo (thesis director), Daugelavicius, Rimantas (thesis director).

Subjects/Keywords: Bactériophage SPP1; Entrée du virus; Ions calcium; YueB; Bacteriophage SPP1; Virus entry; Ca2+ ions; Membrane voltage; Gram-positive

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APA (6^th Edition):

Jakutyte, L. (2011). Bacteriophage SPP1 entry into the host cell : Entrée de bactériophage SPP1 dans la cellule hôte. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2011PA114842

Chicago Manual of Style (16^th Edition):

Jakutyte, Lina. “Bacteriophage SPP1 entry into the host cell : Entrée de bactériophage SPP1 dans la cellule hôte.” 2011. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 25, 2021. http://www.theses.fr/2011PA114842.

MLA Handbook (7^th Edition):

Jakutyte, Lina. “Bacteriophage SPP1 entry into the host cell : Entrée de bactériophage SPP1 dans la cellule hôte.” 2011. Web. 25 Jan 2021.

Vancouver:

Jakutyte L. Bacteriophage SPP1 entry into the host cell : Entrée de bactériophage SPP1 dans la cellule hôte. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2011. [cited 2021 Jan 25]. Available from: http://www.theses.fr/2011PA114842.

Council of Science Editors:

Jakutyte L. Bacteriophage SPP1 entry into the host cell : Entrée de bactériophage SPP1 dans la cellule hôte. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2011. Available from: http://www.theses.fr/2011PA114842

10. NC DOCKS at Western Carolina University; Pathirannahel, Buddhika Liyana. Development of microfluidic paper based analytical devices (µPADS) for the detection of calcium and magnesium ions.

Degree: 2018, NC Docks

URL: http://libres.uncg.edu/ir/wcu/f/Pathirannahel2018.pdf

► Microfluidic paper-based analytical devices (µPADs) have been developed using several patterning technologies to reproducibly create inexpensive lab-on-chip type analytical tools. Consequently, these devices are useful… (more)

▼ Microfluidic paper-based analytical devices (µPADs) have been developed using several patterning technologies to reproducibly create inexpensive lab-on-chip type analytical tools. Consequently, these devices are useful for field deployable measurements in the areas of healthcare diagnostics, environmental monitoring, and forensic analysis. These devices are portable, cost effective and easily reproducible. One of the significant characteristics of µPADs is that they are extremely versatile. Thus, they can be modified to any application, particularly in low resourceful settings. The goal of this project was to create novel microfluidic paper-based analytical devices µPADs that were able to detect and quantify the presence of calcium and magnesium ions in water samples using a simple complexometric titration. The work involved in this project can be divided into two parts. The first part was spent on optimizing parameters that are needed to produce µPAD consistently and repeatedly using wax printing method. The parameters studied are the melting temperature, the melting time and their effect on the barrier width and the channel width. Based on the results that were obtained, µPADs were designed with ten reservoirs in the middle of channels and ten reservoirs at the end of the channels. The detection of the calcium and magnesium was performed on the µPADs using typical complexometric titrations. Two separate titration reactions were carried out on µPADs that were prepared separately. The first step was to determine the total water hardness which is the total concentration of magnesium and calcium ions in water. The pH of the µPADs for this analysis was maintained at pH 10 using CAPS buffer. The ten reaction reservoirs contained various amounts of EDTA, whereas a constant amount of EBT was added to all detection reservoirs. When the amount of total calcium and magnesium ions exceeded that of the EDTA in the reaction reservoirs, free metal ions penetrated the detection reservoirs resulting a color reaction from EBT. Therefore, the first detection reservoir with no color change determined the total concentration of calcium and magnesium ions which is equal to total hardness. Next step was to determine the individual calcium ion concentration at pH 13. The pH on the µPADs was achieved using 10 M KOH. Here the ten reaction reservoirs contained various amounts of EDTA while the detection zone contained a constant amount of calcon as the metal indicator. When a water sample containing calcium and magnesium ions are added to the sample zone, magnesium ions get precipitated as a solid while free calcium ions get penetrated to the reaction and detection zones. Here, the calcium concentration is determined based on the concentration of the first reaction zone with no color change. The effects of other metal ions that might be present in the water on the µPADs were also studied. It was found that the presence of Cu2+, Ni2+, Al3+, Mn2+ and Fe3+ ions could interfere with the results. The µPADs determined the concentration of metal…

Subjects/Keywords: Microfluidic devices – Design and construction; Detectors – Design and construction; Water – Analysis; Calcium ions; Magnesium ions; Water – Hardness

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APA (6^th Edition):

NC DOCKS at Western Carolina University; Pathirannahel, B. L. (2018). Development of microfluidic paper based analytical devices (µPADS) for the detection of calcium and magnesium ions. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/wcu/f/Pathirannahel2018.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

NC DOCKS at Western Carolina University; Pathirannahel, Buddhika Liyana. “Development of microfluidic paper based analytical devices (µPADS) for the detection of calcium and magnesium ions.” 2018. Thesis, NC Docks. Accessed January 25, 2021. http://libres.uncg.edu/ir/wcu/f/Pathirannahel2018.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

NC DOCKS at Western Carolina University; Pathirannahel, Buddhika Liyana. “Development of microfluidic paper based analytical devices (µPADS) for the detection of calcium and magnesium ions.” 2018. Web. 25 Jan 2021.

Vancouver:

NC DOCKS at Western Carolina University; Pathirannahel BL. Development of microfluidic paper based analytical devices (µPADS) for the detection of calcium and magnesium ions. [Internet] [Thesis]. NC Docks; 2018. [cited 2021 Jan 25]. Available from: http://libres.uncg.edu/ir/wcu/f/Pathirannahel2018.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

NC DOCKS at Western Carolina University; Pathirannahel BL. Development of microfluidic paper based analytical devices (µPADS) for the detection of calcium and magnesium ions. [Thesis]. NC Docks; 2018. Available from: http://libres.uncg.edu/ir/wcu/f/Pathirannahel2018.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Mehta,Anita. Influence of calcium and sodium ions on the mechanical responses to various agonists in smooth muscle preparations; -.

Degree: Pharmacy, 2015, Gujarat University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/33465

available newline

-

Advisors/Committee Members: Dave,K.C.

Subjects/Keywords: calcium and sodium ions; muscle preparations

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APA (6^th Edition):

Mehta,Anita. (2015). Influence of calcium and sodium ions on the mechanical responses to various agonists in smooth muscle preparations; -. (Thesis). Gujarat University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/33465

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mehta,Anita. “Influence of calcium and sodium ions on the mechanical responses to various agonists in smooth muscle preparations; -.” 2015. Thesis, Gujarat University. Accessed January 25, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/33465.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mehta,Anita. “Influence of calcium and sodium ions on the mechanical responses to various agonists in smooth muscle preparations; -.” 2015. Web. 25 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Mehta,Anita. Influence of calcium and sodium ions on the mechanical responses to various agonists in smooth muscle preparations; -. [Internet] [Thesis]. Gujarat University; 2015. [cited 2021 Jan 25]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33465.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mehta,Anita. Influence of calcium and sodium ions on the mechanical responses to various agonists in smooth muscle preparations; -. [Thesis]. Gujarat University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33465

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

University of Oxford

12. Dipp, Michelle. The role of calcium sensitisation in hypoxic pulmonary vasoconstriction.

Degree: PhD, 2001, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:d14ca3ef-c5b8-4a4c-b9d4-5a1ee086cb4a ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249581

► This thesis investigates the role of extracellular and intracellular calcium (Ca²⁺) in hypoxic pulmonary vasoconstriction (HPV), in rat and rabbit small pulmonary arteries and also… (more)

▼ This thesis investigates the role of extracellular and intracellular calcium (Ca²⁺) in hypoxic pulmonary vasoconstriction (HPV), in rat and rabbit small pulmonary arteries and also in the isolated ventilated perfused rat lung in situ. 1. Rat and rabbit pulmonary artery rings responded biphasically to a 30min hypoxic challenge (PO₂, 16-21Torr). An initial transient rise in tension (phase 1) was followed by a slow progressive rise (phase 2). Removal of the endothelium had no effect on phase 1 but abolished the rising component of phase 2, leaving a steady 'plateau constriction'. This supports the idea that an endothelial vasoconstrictor mediates the rising component of phase 2 but not phase 1 of HPV. This ramp component, but not phase 1 or the endothelium-independent plateau constriction was abolished by the removal of extracellular Ca²⁺ suggesting extracellular Ca²⁺ is required for the release and/or actions of an endotheliumderived vasoconstrictor. 2. Pre-incubation with ryanodine and caffeine to deplete intracellular sarcoplasmic reticulum (SR) Ca²⁺ stores, abolished the entire hypoxic response regardless of the presence of extracellular Ca²⁺ or of the endothelium. Pre-incubation with cyclopiazonic acid, which blocks the Ca²⁺ ATPase of the sarcoplasmic reticulum (SR) abolished phase 1, but not phase 2, regardless of the presence of the endothelium. This suggests a role for SR Ca²⁺ release. Omitting pretone (1ìM PGF2á) reduced, but did not otherwise modify these responses. 3. Measurements of cyclic ADP ribose (cADPR) levels using, acid extraction from rabbit pulmonary artery rings, showed that cADPR levels rise during hypoxia (16- 21Torr), but are significantly lower during severe hypoxia (5-8Torr) or normoxia (154-160Torr). In isolated arteries, the plateau constriction, but not phase 1 or the rising component of phase 2, was shown to be cADPR-sensitive. 4. When perfusate from the isolated hypoxic rat lung was applied to de-endothelialised artery rings, tension rose with a time course similar to that of phase 2 of HPV. The pharmacology of the vasoconstrictors) in the perfusate differs from that of ET-1. Constriction by the perfusate was inhibited by a Rhoassociated kinase antagonist, suggesting a role for myofilament Ca²⁺ sensitisation via a Rho-associated kinase. 5. Perfusate taken from the lung after the hypoxic challenge does not constrict the artery to the same degree as perfusate from the hypoxic lung suggesting that the vasoconstrictor is metabolised or removed during normoxia.

Subjects/Keywords: 612; Calcium ions; Physiological effect; Arteritis

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APA (6^th Edition):

Dipp, M. (2001). The role of calcium sensitisation in hypoxic pulmonary vasoconstriction. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:d14ca3ef-c5b8-4a4c-b9d4-5a1ee086cb4a ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249581

Chicago Manual of Style (16^th Edition):

Dipp, Michelle. “The role of calcium sensitisation in hypoxic pulmonary vasoconstriction.” 2001. Doctoral Dissertation, University of Oxford. Accessed January 25, 2021. http://ora.ox.ac.uk/objects/uuid:d14ca3ef-c5b8-4a4c-b9d4-5a1ee086cb4a ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249581.

MLA Handbook (7^th Edition):

Dipp, Michelle. “The role of calcium sensitisation in hypoxic pulmonary vasoconstriction.” 2001. Web. 25 Jan 2021.

Vancouver:

Dipp M. The role of calcium sensitisation in hypoxic pulmonary vasoconstriction. [Internet] [Doctoral dissertation]. University of Oxford; 2001. [cited 2021 Jan 25]. Available from: http://ora.ox.ac.uk/objects/uuid:d14ca3ef-c5b8-4a4c-b9d4-5a1ee086cb4a ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249581.

Council of Science Editors:

Dipp M. The role of calcium sensitisation in hypoxic pulmonary vasoconstriction. [Doctoral Dissertation]. University of Oxford; 2001. Available from: http://ora.ox.ac.uk/objects/uuid:d14ca3ef-c5b8-4a4c-b9d4-5a1ee086cb4a ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249581

Brock University

13. McMahon, John Christopher. A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons .

Degree: Department of Biological Sciences, 1993, Brock University

URL: http://hdl.handle.net/10464/1754

► Increasing the impulse activity of neurons in vivo over 3 or more days causes a reduction in transmitter release that persists for days or weeks… (more)

Subjects/Keywords: Neurons.; Neuroplasticity.; Crayfish – Physiology.; Calcium ions.

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APA (6^th Edition):

McMahon, J. C. (1993). A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/1754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McMahon, John Christopher. “A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons .” 1993. Thesis, Brock University. Accessed January 25, 2021. http://hdl.handle.net/10464/1754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McMahon, John Christopher. “A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons .” 1993. Web. 25 Jan 2021.

Vancouver:

McMahon JC. A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons . [Internet] [Thesis]. Brock University; 1993. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10464/1754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McMahon JC. A possible role for intrinsic calcium buffers in the long- term adaptation of crayfish neurons . [Thesis]. Brock University; 1993. Available from: http://hdl.handle.net/10464/1754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

14. Mills, John Steven. Interaction of calcium, metal ions, and calmodulin antagonist drugs and target proteins with calmodulin.

Degree: PhD, Graduate School, 1987, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu148732574071875

Subjects/Keywords: Chemistry; Calmodulin; Calcium; Metal ions; Proteins

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APA (6^th Edition):

Mills, J. S. (1987). Interaction of calcium, metal ions, and calmodulin antagonist drugs and target proteins with calmodulin. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu148732574071875

Chicago Manual of Style (16^th Edition):

Mills, John Steven. “Interaction of calcium, metal ions, and calmodulin antagonist drugs and target proteins with calmodulin.” 1987. Doctoral Dissertation, The Ohio State University. Accessed January 25, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu148732574071875.

MLA Handbook (7^th Edition):

Mills, John Steven. “Interaction of calcium, metal ions, and calmodulin antagonist drugs and target proteins with calmodulin.” 1987. Web. 25 Jan 2021.

Vancouver:

Mills JS. Interaction of calcium, metal ions, and calmodulin antagonist drugs and target proteins with calmodulin. [Internet] [Doctoral dissertation]. The Ohio State University; 1987. [cited 2021 Jan 25]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu148732574071875.

Council of Science Editors:

Mills JS. Interaction of calcium, metal ions, and calmodulin antagonist drugs and target proteins with calmodulin. [Doctoral Dissertation]. The Ohio State University; 1987. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu148732574071875

15. Xia, Fan. Décryptage des mécanismes de signalisation précoce de la costimulation dans l' activation des lymphocytes T naifs : Deciphering the mechanisms of TCR and CD28 early signaling pathway cooperation required for naïve T cell activation.

Degree: Docteur es, Immunologie, 2014, Aix Marseille Université

URL: http://www.theses.fr/2014AIXM4064

►

L'objectif de notre travail est de comprendre la contribution relative des voies de signalisation précoces du TCR et de CD28 dans l'activation des lymphocytes T… (more)

▼

L'objectif de notre travail est de comprendre la contribution relative des voies de signalisation précoces du TCR et de CD28 dans l'activation des lymphocytes T naïfs. Notre étude a d'abord montré que dans les cellules T CD4+ naïves, la stimulation du TCR augmente de manière significative la liaison en deux dimensions (2D) de CD28 avec ses ligands B7, et ceci dépend à la fois du domaine cytoplasmique de CD28 et de l'activité des src kinases. Par la suite, notre analyse biochimique a démontré que l'engagement du TCR par son ligand (CMHp) potentialise la phosphorylation de CD28 stimulée par son ligand B7. En outre, la stimulation conjointe du TCR et de CD28 augmente fortement la phosphorylation des protéines de signalisation proximales telles que les molécules Vav-1 et PLCγ-1. Nous avons également examiné la mobilisation des ions calcique (Ca++). Nous avons trouvé que l'engagement du TCR ou de CD28 seul est capable de déclencher une élévation de la concentration intracellulaire d'ions Ca++ dans des cellules T naïves. Cette élévation qui se caractérise par de fortes fluctuations de la concentration calcique impliquerait principalement 2 types de canaux calciques de la membrane plasmique. De façon attendue, une stimulation conjointe des lymphocytes par le TCR et CD28 augmente l'amplitude moyenne de la réponse calcique. Nos données ont révélé que seule une stimulation conjointe, et non individuelle, du TCR et de CD28 augmente significativement le temps de résidence du Ca++ libres fluctuants par rapport aux cellules non stimulées. Par conséquent, cette augmentation du temps de résidence caractérise spécifiquement la réponse calcique induite par TCR et CD28.

In this work, we aimed at determining the relationship between and specific contribution of TCR and CD28 early signaling pathways in naïve CD4+ T cell activation. Our data showed that in naïve CD4+ T cells, TCR stimulation significantly increased the 2D binding of CD28 to its B7 ligands and this increase depended on both cytoplasmic tail of CD28 and activity of src kinases. Our biochemical analysis then demonstrated that TCR engagement with its ligand pMHC strongly enhanced the CD28 tyrosine phosphorylation triggered by B7. Moreover, the conjoint stimulation of TCR and CD28 markedly augmented activation of proximal signaling molecules such like Vav-1 and PLCγ-1 compared to the stimulation with each receptor alone. We next went to examine the calcium ion (Ca2+) mobilization. We found that in naïve CD4+ T cells, engagement with ligand of TCR or CD28 alone was able to trigger rise of the fluctuating cytosolic-free Ca2+ level. Unexpectedly, such rises implicated predominantly the involvements of two different types of calcium channels: Cav and CRAC channels. The conjoint stimulation with both TCR and CD28 enabled the augment of average amplitude of the calcium response. Through the time series analysis, our data unveiled that the conjoint, but not separate, TCR and CD28 stimulation in naïve CD4+ T cells significantly increased the fluctuating cytosolic-free Ca2+ dwell…

Advisors/Committee Members: He, Hai Tao (thesis director).

Subjects/Keywords: Tcr; Cd28; Costimulation; La mobilisation des ions calcique; Lymphocytes T naïfs; Tcr; Cd28; Costimulation; Calcium mobilization; Naïve T cell; 571

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APA (6^th Edition):

Xia, F. (2014). Décryptage des mécanismes de signalisation précoce de la costimulation dans l' activation des lymphocytes T naifs : Deciphering the mechanisms of TCR and CD28 early signaling pathway cooperation required for naïve T cell activation. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2014AIXM4064

Chicago Manual of Style (16^th Edition):

Xia, Fan. “Décryptage des mécanismes de signalisation précoce de la costimulation dans l' activation des lymphocytes T naifs : Deciphering the mechanisms of TCR and CD28 early signaling pathway cooperation required for naïve T cell activation.” 2014. Doctoral Dissertation, Aix Marseille Université. Accessed January 25, 2021. http://www.theses.fr/2014AIXM4064.

MLA Handbook (7^th Edition):

Xia, Fan. “Décryptage des mécanismes de signalisation précoce de la costimulation dans l' activation des lymphocytes T naifs : Deciphering the mechanisms of TCR and CD28 early signaling pathway cooperation required for naïve T cell activation.” 2014. Web. 25 Jan 2021.

Vancouver:

Xia F. Décryptage des mécanismes de signalisation précoce de la costimulation dans l' activation des lymphocytes T naifs : Deciphering the mechanisms of TCR and CD28 early signaling pathway cooperation required for naïve T cell activation. [Internet] [Doctoral dissertation]. Aix Marseille Université 2014. [cited 2021 Jan 25]. Available from: http://www.theses.fr/2014AIXM4064.

Council of Science Editors:

Xia F. Décryptage des mécanismes de signalisation précoce de la costimulation dans l' activation des lymphocytes T naifs : Deciphering the mechanisms of TCR and CD28 early signaling pathway cooperation required for naïve T cell activation. [Doctoral Dissertation]. Aix Marseille Université 2014. Available from: http://www.theses.fr/2014AIXM4064

Hong Kong University of Science and Technology

16. Lee, Karen Wing-man. A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos.

Degree: 2004, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-2375 ; https://doi.org/10.14711/thesis-b843336 ; http://repository.ust.hk/ir/bitstream/1783.1-2375/1/th_redirect.html

► It has previously been shown that four Ca2+ transients, generated by release from intracellular stores, are associated with cytokinesis during the early cell division cycles… (more)

▼ It has previously been shown that four Ca2+ transients, generated by release from intracellular stores, are associated with cytokinesis during the early cell division cycles of zebrafish embryos (Webb et al., 1997; Chang and Lu, 2000; Lee et al., 2003). These have been termed the furrow positioning, propagation, deepening and apposition transients. Here, I clearly demonstrate that the furrow positioning Ca2+ transient precedes the appearance of the cleavage furrow on the surface of the blastodisc, suggesting, therefore, that it is a key component of the furrow positioning signal. Through the introduction of the Ca2+ shuttle buffer, dibromo-BAPTA, at specific times to challenge the individual transients, I demonstrate the requirement of the four Ca2+ transients in the furrow positioning, propagation, deepening and apposition processes. Pharmacological studies show that all four transients are generated by Ca2+ released via inositol 1,4,5-trisphosphate (IP3) receptors and not via ryanodine receptors or NAADP-sensitive channels. In addition, microinjection of IP3 results in the release of Ca2+ via IP3-sensitive stores. Furthermore, by labeling the endoplasmic reticulum and IP3 receptors by immunocytochemistry, I demonstrate that they are initially both localized in the blastodisc cortex at the specific position of the future cleavage furrow; i.e., they appear prior to the physical appearance of the furrow at the blastodisc surface. As the furrow ingresses, they are then localized on either side of the deepening furrow. These new data thus provide additional evidence that the ER and IP3 receptors are the intracellular Ca2+-store and release mechanisms, respectively, for generating all the cytokinetic transients. I also report evidence to suggest that during the first few cell division cycles in zebrafish embryos, a dynamic population of central-spindle microtubules serve a major function in positioning the cleavage furrow at the blastoderm surface. Originating from the midzone of the mitotic spindle, they develop into a mid-spindle 'pre-furrowing microtubule array' (pf-MTA) that expands upward and outward from the spindle midzone to the blastodisc surface. I suggest that this pf-MTA transmits positional information from the mitotic spindle to the blastodisc cortex that results in the correctly positioned assembly of the cytokinetic contractile apparatus. It is proposed that the pf-MTA is also involved in localizing the corticular ER to the cleavage plane, thus providing a mechanistic link between the mitotic spindle and the series of cytokinetic Ca2+ transients. I also suggest that the pf-MTA then develops into a furrow-ingression microtubule array that helps to direct and assemble the deepening furrow as it cuts its way through the blastodisc. It then also plays a role in transporting key components to facilitate furrow apposition. Finally, I provide evidence for some of the possible downstream targets of the cytokinetic Ca2+ transients. I show that the furrow propagation Ca2+ transient co-localizes with a region of…

Subjects/Keywords: Calcium ions – Physiological effect ; Zebra danio – Embryos ; Cytokinesis

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APA (6^th Edition):

Lee, K. W. (2004). A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-2375 ; https://doi.org/10.14711/thesis-b843336 ; http://repository.ust.hk/ir/bitstream/1783.1-2375/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Karen Wing-man. “A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos.” 2004. Thesis, Hong Kong University of Science and Technology. Accessed January 25, 2021. http://repository.ust.hk/ir/Record/1783.1-2375 ; https://doi.org/10.14711/thesis-b843336 ; http://repository.ust.hk/ir/bitstream/1783.1-2375/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Karen Wing-man. “A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos.” 2004. Web. 25 Jan 2021.

Vancouver:

Lee KW. A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2004. [cited 2021 Jan 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-2375 ; https://doi.org/10.14711/thesis-b843336 ; http://repository.ust.hk/ir/bitstream/1783.1-2375/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee KW. A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos. [Thesis]. Hong Kong University of Science and Technology; 2004. Available from: http://repository.ust.hk/ir/Record/1783.1-2375 ; https://doi.org/10.14711/thesis-b843336 ; http://repository.ust.hk/ir/bitstream/1783.1-2375/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

17. Wilson, Michael Alan. Solubility of rock phosphates as influenced by calcium ion activity in solution and surface area.

Degree: MS, Department of Crop and Soil Sciences, 1979, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:35255

Subjects/Keywords: Phosphate rock – Solubility; Phosphate rock; Calcium ions

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APA (6^th Edition):

Wilson, M. A. (1979). Solubility of rock phosphates as influenced by calcium ion activity in solution and surface area. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:35255

Chicago Manual of Style (16^th Edition):

Wilson, Michael Alan. “Solubility of rock phosphates as influenced by calcium ion activity in solution and surface area.” 1979. Masters Thesis, Michigan State University. Accessed January 25, 2021. http://etd.lib.msu.edu/islandora/object/etd:35255.

MLA Handbook (7^th Edition):

Wilson, Michael Alan. “Solubility of rock phosphates as influenced by calcium ion activity in solution and surface area.” 1979. Web. 25 Jan 2021.

Vancouver:

Wilson MA. Solubility of rock phosphates as influenced by calcium ion activity in solution and surface area. [Internet] [Masters thesis]. Michigan State University; 1979. [cited 2021 Jan 25]. Available from: http://etd.lib.msu.edu/islandora/object/etd:35255.

Council of Science Editors:

Wilson MA. Solubility of rock phosphates as influenced by calcium ion activity in solution and surface area. [Masters Thesis]. Michigan State University; 1979. Available from: http://etd.lib.msu.edu/islandora/object/etd:35255

University of Florida

18. Moloye, Titilayo Ayomi. Composite Scaffolds for Bone Tissue Engineering.

Degree: PhD, Biomedical Engineering, 2012, University of Florida

URL: https://ufdc.ufl.edu/UFE0044643

► Diseases, accidents, and aging are the leading causes of bone defects that can weaken the bone’s natural anatomy and, as a result, impair its function.… (more)

▼ Diseases, accidents, and aging are the leading causes of bone defects that can weaken the bone’s natural anatomy and, as a result, impair its function. 5-10% of the 6 million defects occurring each year require further treatment for compromised healing due to loss of bone, metabolic disturbances, and impairment of blood supply. Currently, the standard solution for fixing such defects requires multiple surgeries and the removal of healthy tissue. An effective and viable tissue-engineered bone scaffold would provide a platform for bone formation as well as an intrinsic signal for pluripotent cells to differentiate into bone forming cells. In this study, a composite scaffold of polycaprolactone (PCL) and demineralized bone matrix (DBM) is proposed, using a solvent casting and particulate leaching technique. The broad hypothesis behind the construct of this composite scaffold was simple; the combination of an osteoinductive/osteoconductive material coupled with a biocompatible polymer would yield a scaffold that 1) delivers growth factors to a targeted site, 2) encourages and enhances the formation of mineral deposits on its surface, and 3) allows the proliferation and differentiation of undifferentiated cells on its surface. Initially, composite scaffolds of three different DBM concentrations, 25, 35, and 50 wt %, were prepared to evaluate scaffold degradation and surface mineralization. Scaffolds containing 35% DBM both loss and gained mass over the 7 week study. This gain was likely due to mineral being deposited on its surface. Moreover, analysis of the X ray diffraction patterns of the DBM particles before and after immersion in SBF revealed reprecipitation on its surface after 20 day However, despite the additional ions released from the DBM into the simulated body fluid available for reprecipitation, we report no correlation in DBM concentration and surface mineralization. Lastly, analytical assays of alkaline phosphatase and total calcium content revealed high values of expression on composite scaffolds when compared to the pure PCL scaffolds The work described herein provides strong support for a composite scaffold with the potential of bonding to bone in vivo and inducing the differentiation of undifferentiated cells into an osteoblastic lineage. ( en ) Advisors/Committee Members: Batich, Christopher D (committee chair), Banks, Scott A (committee member), Keselowsky, Ben (committee member), Ghivizzani, Steven (committee member).

Subjects/Keywords: Bones; Calcium; Cells; Ions; Minerals; Osteoblasts; Polymers; Scaffolds; Tissue engineering; Tissue grafting; biomaterials – bone – engineering – scaffolds – tissue

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APA (6^th Edition):

Moloye, T. A. (2012). Composite Scaffolds for Bone Tissue Engineering. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0044643

Chicago Manual of Style (16^th Edition):

Moloye, Titilayo Ayomi. “Composite Scaffolds for Bone Tissue Engineering.” 2012. Doctoral Dissertation, University of Florida. Accessed January 25, 2021. https://ufdc.ufl.edu/UFE0044643.

MLA Handbook (7^th Edition):

Moloye, Titilayo Ayomi. “Composite Scaffolds for Bone Tissue Engineering.” 2012. Web. 25 Jan 2021.

Vancouver:

Moloye TA. Composite Scaffolds for Bone Tissue Engineering. [Internet] [Doctoral dissertation]. University of Florida; 2012. [cited 2021 Jan 25]. Available from: https://ufdc.ufl.edu/UFE0044643.

Council of Science Editors:

Moloye TA. Composite Scaffolds for Bone Tissue Engineering. [Doctoral Dissertation]. University of Florida; 2012. Available from: https://ufdc.ufl.edu/UFE0044643

University of Florida

19. Indarawis, Katrina Anne. Investigation of Combined Ion Exchange to Decrease Membrane Fouling Potential during Water Treatment.

Degree: PhD, Environmental Engineering Sciences, 2013, University of Florida

URL: https://ufdc.ufl.edu/UFE0045939

► The goal of this research was to determine if combined cation and anion exchange would reduce fouling of reverse osmosis membranes. This research is divided… (more)

▼ The goal of this research was to determine if combined cation and anion exchange would reduce fouling of reverse osmosis membranes. This research is divided into three parts. The first part provides an improved understanding of the interactions between alkaline earth metals and dissolved organic matter (DOM) under conditions that are encountered during drinking water treatment with particular focus on cation exchange. Both magnetically-enhanced and nonmagnetic cation exchange resins were converted to Na, Mg, Ca, Sr, and Ba mobile counterion forms as a novel approach to investigating the exchange behavior of the cations and the interactions between the cations and DOM. The results show that cation exchange is a robust process for removal of Ca2+and Mg2+ considering competition between the cations on the resin surface and presence of DOM. The second part of this research focuses on combined ion exchange and answers the research question, “Can the effects of combined ion exchange be predicted as the sum of anions removed during anion exchange and cations removed during cation exchange?.” Results indicated that combined ion exchange can reduce precipitation of sparingly soluble minerals, such as CaCO3, from the bulk solution by simultaneously removing calcium and bicarbonate. Results showed that it is possible to estimate sulfate, magnesium, and DOC removal during combined ion exchange by rules of superposition, as well as calcium when the initial concentration of calcium is low. The effect of combined ion exchange on separation factors is also calculated and discussed. The third part of this research compares anion exchange pretreatment, cation exchange pretreatment, and combined ion exchange pretreatment to a natural groundwater before reverse osmosis (RO). This was to determine which ion exchange pretreatment reduced fouling of RO, quantitatively determined by flux decline. It was shown that all ion exchange pretreatments yielded similar rates of flux decline, however anion exchange pretreated samples yielded overall lower flux. Therefore, removal of divalent cations could result in a decrease in fouling of RO compared to the removal of DOM. ( en ) Advisors/Committee Members: Boyer, Treavor H (committee chair), Chadik, Paul A (committee member), Mazyck, David W (committee member), Zimmerman, Andrew R (committee member).

Subjects/Keywords: Anions; Calcium; Cations; Fouling; Groundwater; Ions; Pretreatment; Resins; Water quality; Water tables; combined – exchange – ion – pretreatment

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APA (6^th Edition):

Indarawis, K. A. (2013). Investigation of Combined Ion Exchange to Decrease Membrane Fouling Potential during Water Treatment. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0045939

Chicago Manual of Style (16^th Edition):

Indarawis, Katrina Anne. “Investigation of Combined Ion Exchange to Decrease Membrane Fouling Potential during Water Treatment.” 2013. Doctoral Dissertation, University of Florida. Accessed January 25, 2021. https://ufdc.ufl.edu/UFE0045939.

MLA Handbook (7^th Edition):

Indarawis, Katrina Anne. “Investigation of Combined Ion Exchange to Decrease Membrane Fouling Potential during Water Treatment.” 2013. Web. 25 Jan 2021.

Vancouver:

Indarawis KA. Investigation of Combined Ion Exchange to Decrease Membrane Fouling Potential during Water Treatment. [Internet] [Doctoral dissertation]. University of Florida; 2013. [cited 2021 Jan 25]. Available from: https://ufdc.ufl.edu/UFE0045939.

Council of Science Editors:

Indarawis KA. Investigation of Combined Ion Exchange to Decrease Membrane Fouling Potential during Water Treatment. [Doctoral Dissertation]. University of Florida; 2013. Available from: https://ufdc.ufl.edu/UFE0045939

University of Florida

20. Smith, Shanna M. Polymeric Chelators for Pharmaceutical Applications.

Degree: PhD, Materials Science and Engineering, 2016, University of Florida

URL: https://ufdc.ufl.edu/UFE0050384

► The development of bone-seeking bisphosphonates has allowed for more targeted approaches in treating pediatric osteosarcoma such as the radiopharmaceutical 153Sm-EDTMP, but treatment is limited in… (more)

Subjects/Keywords: Calcium; Calibration; Copolymers; Ions; Kidney dialysis; pH; Phosphates; Phosphonic acids; Polymers; Samarium; chitosan – hyperphosphatemia – ligand – osteosarcoma – radiopharmaceutical

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APA (6^th Edition):

Smith, S. M. (2016). Polymeric Chelators for Pharmaceutical Applications. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0050384

Chicago Manual of Style (16^th Edition):

Smith, Shanna M. “Polymeric Chelators for Pharmaceutical Applications.” 2016. Doctoral Dissertation, University of Florida. Accessed January 25, 2021. https://ufdc.ufl.edu/UFE0050384.

MLA Handbook (7^th Edition):

Smith, Shanna M. “Polymeric Chelators for Pharmaceutical Applications.” 2016. Web. 25 Jan 2021.

Vancouver:

Smith SM. Polymeric Chelators for Pharmaceutical Applications. [Internet] [Doctoral dissertation]. University of Florida; 2016. [cited 2021 Jan 25]. Available from: https://ufdc.ufl.edu/UFE0050384.

Council of Science Editors:

Smith SM. Polymeric Chelators for Pharmaceutical Applications. [Doctoral Dissertation]. University of Florida; 2016. Available from: https://ufdc.ufl.edu/UFE0050384

21. Prusti, Rabindra Kumar. The role of calcium ions in photosensory transduction and the photodynamic action in Stentor coeruleus.

Degree: Chemistry, 1987, Texas Tech University

URL: http://hdl.handle.net/2346/10727

Subjects/Keywords: Photoreceptors; Stentor coeruleus; Calcium ions; Transduction; Stentorin

…concentration of phosphatidic acid in the presence of 0, 2.5, and 5.0 mM calcium (redrawn frora… …l followmg the priraary photoprocess(es), opening of the calcium channels and…

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APA (6^th Edition):

Prusti, R. K. (1987). The role of calcium ions in photosensory transduction and the photodynamic action in Stentor coeruleus. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/10727

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prusti, Rabindra Kumar. “The role of calcium ions in photosensory transduction and the photodynamic action in Stentor coeruleus.” 1987. Thesis, Texas Tech University. Accessed January 25, 2021. http://hdl.handle.net/2346/10727.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prusti, Rabindra Kumar. “The role of calcium ions in photosensory transduction and the photodynamic action in Stentor coeruleus.” 1987. Web. 25 Jan 2021.

Vancouver:

Prusti RK. The role of calcium ions in photosensory transduction and the photodynamic action in Stentor coeruleus. [Internet] [Thesis]. Texas Tech University; 1987. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/2346/10727.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prusti RK. The role of calcium ions in photosensory transduction and the photodynamic action in Stentor coeruleus. [Thesis]. Texas Tech University; 1987. Available from: http://hdl.handle.net/2346/10727

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

22. Jee, Sang. Development of Collagen-Hydroxyapatite Nanostructured Composites via a Calcium Phosphate Precursor Mechanism.

Degree: PhD, Materials Science and Engineering, 2008, University of Florida

URL: https://ufdc.ufl.edu/UFE0022441

► Bone is an interpenetrating inorganic/organic composite that consists of mineralized collagen fibrils, which is hierarchically organized into various structures. The structure of mineralized collagen fibril,… (more)

▼ Bone is an interpenetrating inorganic/organic composite that consists of mineralized collagen fibrils, which is hierarchically organized into various structures. The structure of mineralized collagen fibril, in which nano-crystals of hydroxyapatite are embedded within the collagen fibrils, provides remarkable mechanical and bio-resorptive properties. Therefore, there have been many attempts to produce collagen-hydroxyapatite composites having a bone-like structure. However, duplication of even the most fundamental level of bone structure has not been easily achieved by conventional nucleation and growth techniques, which are based on the most widely accepted hypothesis of bone mineralization. In nature, the collagen fibril is mineralized via intrafibrillar mineralization, which produces preferentially oriented hydroxyapatite nano-crystals occupying the interstices in collagen fibrils. Our group has demonstrated that intrafibrillar mineralization can be achieved by using a new method based on the Polymer-Induced Liquid-Precursor (PILP) mineralization process. In the PILP process, a poly-anionic additive can produce an amorphous calcium phosphate precursor which enables us to achieve intrafibrillar mineralization of collagen. It is thought that the precursor is pulled into the interstices of the collagen fibrils via capillary forces, and upon solidification and crystallization of the precursor produces an interpenetrating composite with the nanostructured architecture of bone. In this dissertation, to demonstrate the effectiveness of the PILP process on the intrafibrillar mineralization of collagen fibril, various collagen scaffolds, such as turkey tendon, bovine tendon and synthetic collagen sponge, were mineralized by the PILP process. Various poly-aspartates with different molecular weight were also used for the optimization of the PILP process for the mineralization of the collagen scaffolds. With the systematic researches, we discovered that the molecular weight of poly-aspartic acid affects the degree of intrafibrillar mineralization of collagen scaffolds. High molecular weight poly-aspartic acid could produce a stable and dispersed amorphous precursor, leading to a high degree of intrafibrillar mineralization. The mineral content of the collagen sponge mineralized using high molecular weight poly-aspartic acid was equivalent to the mineral content of bone. According to X-ray diffraction analysis of the mineralized collagen, the size and composition of the intrafibrillar hydroxyapatite produced by the PILP process were almost identical to carbonated hydroxyapatite in bone. The selective area electron diffraction patterns indicated that the [001] direction of hydroxyapatite is roughly aligned along the c-axis of collagen fibril, leading to the formation 002 arcs. Using dark field imaging, it was possible to visualize the preferentially oriented hydroxyapatite in TEM. Thermal analysis of mineralized collagen also showed a reduction in the thermal stability of collagen, which is similar to that observed in the… Advisors/Committee Members: Gower, Laurie B. (committee chair), Douglas, Elliot P. (committee member), Mecholsky, John J. (committee member), Sigmund, Wolfgang M. (committee member), Long, Joanna R. (committee member).

Subjects/Keywords: Bones; Calcium; Calcium phosphates; Cattle; Collagens; Ions; Molecular weight; Polymers; Solar fibrils; Tendons

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APA (6^th Edition):

Jee, S. (2008). Development of Collagen-Hydroxyapatite Nanostructured Composites via a Calcium Phosphate Precursor Mechanism. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0022441

Chicago Manual of Style (16^th Edition):

Jee, Sang. “Development of Collagen-Hydroxyapatite Nanostructured Composites via a Calcium Phosphate Precursor Mechanism.” 2008. Doctoral Dissertation, University of Florida. Accessed January 25, 2021. https://ufdc.ufl.edu/UFE0022441.

MLA Handbook (7^th Edition):

Jee, Sang. “Development of Collagen-Hydroxyapatite Nanostructured Composites via a Calcium Phosphate Precursor Mechanism.” 2008. Web. 25 Jan 2021.

Vancouver:

Jee S. Development of Collagen-Hydroxyapatite Nanostructured Composites via a Calcium Phosphate Precursor Mechanism. [Internet] [Doctoral dissertation]. University of Florida; 2008. [cited 2021 Jan 25]. Available from: https://ufdc.ufl.edu/UFE0022441.

Council of Science Editors:

Jee S. Development of Collagen-Hydroxyapatite Nanostructured Composites via a Calcium Phosphate Precursor Mechanism. [Doctoral Dissertation]. University of Florida; 2008. Available from: https://ufdc.ufl.edu/UFE0022441

University of British Columbia

23. Minocherhomjee, A. M. Anion regulation of Ca2+ transport ATPase of the human erythrocyte membrane.

Degree: PhD, Pharmaceutical Sciences, 1982, University of British Columbia

URL: http://hdl.handle.net/2429/23912

► The mechanism of regulation of the Ca²⁺ pump ATPase of the human erythrocyte membrane by calmodulin, cyclic AMP and the anion channel was studied using… (more)

▼ The mechanism of regulation of the Ca²⁺ pump ATPase of the human erythrocyte membrane by calmodulin, cyclic AMP and the anion channel was studied using membrane fragments, resealed "ghosts", inside-out vesicles and a Triton X-100 solubilized enzyme preparation. The (Ca²⁺ + Mg²⁺ )-ATPase activity in erythrocyte membranes or a Triton X-100 solubilized enzyme preparation showed biphasic (high and low affinity) Ca²⁺ activation kinetics. The anionic calcium binding protein, calmodulin, increased both the calcium sensitivity (Kca²⁺) and the maximum velocity (Vmax ) of the enzyme. Certain polyanionic agents (poly-L-aspartic acid, poly-L-glutamic acid), alicyclic sulfonic acids (HEPES,N-2-hydroxyethylpiperazine-N¹-2-ethanesulfonic acid, MES,2-N- (morpholinoethanesulfonic acid)), and aromatic carboxylic acids (benzoic and salicylic acids) increased the Kca²⁺ but not the Vmax of (Ca²⁺ + Mg²⁺ )-ATPase in erythrocyte membranes and Triton X-100 solubilized enzyme preparations. Trifluoperazine (30 μM) antagonized activation of the enzyme by calmodulin and poly-L-aspartic acid, but not by sodium-HEPES or sodium-MES. Limited trypsin proteolysis of (Ca²⁺ + Mg²⁺ )-ATPase in the erythrocyte membrane abolished activation by calmodulin, poly-L-aspartic acid and sodium-HEPES. These results suggest that the modulation of the Ca²⁺ sensitivity of (Ca²⁺ + Mg²⁺ )-ATPase by calmodulin may be associated with the anionic properties of this protein, and that this property can be mimicked by some other anions, probably by interacting at an anion-regulatory site on the enzyme. Cyclic AMP (5 μM) was found to inhibit the (Ca²⁺ + Mg²⁺)-ATPase activity (approx. 20%) in erythrocyte membranes, probably via endogenous cyclic AMP protein kinase, since this effect could be blocked by cyclic AMP protein kinase inhibitor (PKI) from the rabbit skeletal muscle, By contrast, bovine heart PKI stimulated (Ca²⁺ + Mg²⁺ )-ATPase activity (approx. 100%) by increasing the Kca²⁺ but not the Vmax of the enzyme in membrane or Triton X-100 solubilized preparations. At a low calcium concentration the stimulation by bovine heart PKI and saturating levels of calmodulin was additive, suggesting that the two effectors acted by distinct mechanisms. The stimulation of (Ca²⁺ + Mg²⁺ )-ATPase activity by bovine heart PKI was not solely due to its antagonism of the protein kinase because a) modification of arginine residues of bovine heart PKI abolished its inhibition of cyclic AMP protein kinase, but had no effect on the stimulation of (Ca²⁺ + Mg²⁺ )-ATPase; b) trifluoperazine (20 μM) antagonized the stimulation of (Ca²⁺ + Mg²⁺ )-ATPase by PKI, similarly to its antagonism of calmodulin stimulation, but it did not affect the inhibition of protein kinase by PKI. It is suggested that different mechanisms are involved in the inhibition of cyclic AMP protein kinase and the stimulation of (Ca²⁺ + Mg²⁺ )-ATPase by bovine heart cyclic AMP PKI. Next, the role of anion channel blockers on the (Ca²⁺ + Mg²⁺ )- ATPase was studied. The photolabeling reagent…

Subjects/Keywords: Ions; Calcium in the body; Erythrocytes; Adenosine triphosphatase; Calcium-Transporting ATPases

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APA (6^th Edition):

Minocherhomjee, A. M. (1982). Anion regulation of Ca2+ transport ATPase of the human erythrocyte membrane. (Doctoral Dissertation). University of British Columbia. Retrieved from http://hdl.handle.net/2429/23912

Chicago Manual of Style (16^th Edition):

Minocherhomjee, A M. “Anion regulation of Ca2+ transport ATPase of the human erythrocyte membrane.” 1982. Doctoral Dissertation, University of British Columbia. Accessed January 25, 2021. http://hdl.handle.net/2429/23912.

MLA Handbook (7^th Edition):

Minocherhomjee, A M. “Anion regulation of Ca2+ transport ATPase of the human erythrocyte membrane.” 1982. Web. 25 Jan 2021.

Vancouver:

Minocherhomjee AM. Anion regulation of Ca2+ transport ATPase of the human erythrocyte membrane. [Internet] [Doctoral dissertation]. University of British Columbia; 1982. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/2429/23912.

Council of Science Editors:

Minocherhomjee AM. Anion regulation of Ca2+ transport ATPase of the human erythrocyte membrane. [Doctoral Dissertation]. University of British Columbia; 1982. Available from: http://hdl.handle.net/2429/23912

Utah State University

24. Pavenayotin, Nuchanart. Effect of Added Calcium Ions on Relative Milk-Clotting Activities of Commercial Milk Clotting Enzymes.

Degree: MS, Nutrition, Dietetics, and Food Sciences, 1974, Utah State University

URL: https://digitalcommons.usu.edu/etd/5140

► Effect of added calcium ions on the relative milk clotting activities of porcine pepsin, Mucor miehei protease, Endothia parasitica protease, and Mucor pusillus protease… (more)

Subjects/Keywords: Added Calcium; Calcium Ions; Relative Milk Clotting; Milk Clotting; Clotting Enzymes; Food Chemistry

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APA (6^th Edition):

Pavenayotin, N. (1974). Effect of Added Calcium Ions on Relative Milk-Clotting Activities of Commercial Milk Clotting Enzymes. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/5140

Chicago Manual of Style (16^th Edition):

Pavenayotin, Nuchanart. “Effect of Added Calcium Ions on Relative Milk-Clotting Activities of Commercial Milk Clotting Enzymes.” 1974. Masters Thesis, Utah State University. Accessed January 25, 2021. https://digitalcommons.usu.edu/etd/5140.

MLA Handbook (7^th Edition):

Pavenayotin, Nuchanart. “Effect of Added Calcium Ions on Relative Milk-Clotting Activities of Commercial Milk Clotting Enzymes.” 1974. Web. 25 Jan 2021.

Vancouver:

Pavenayotin N. Effect of Added Calcium Ions on Relative Milk-Clotting Activities of Commercial Milk Clotting Enzymes. [Internet] [Masters thesis]. Utah State University; 1974. [cited 2021 Jan 25]. Available from: https://digitalcommons.usu.edu/etd/5140.

Council of Science Editors:

Pavenayotin N. Effect of Added Calcium Ions on Relative Milk-Clotting Activities of Commercial Milk Clotting Enzymes. [Masters Thesis]. Utah State University; 1974. Available from: https://digitalcommons.usu.edu/etd/5140

25. Winegar, Bruce D. (Bruce David). Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction.

Degree: 1986, North Texas State University

URL: https://digital.library.unt.edu/ark:/67531/metadc331287/

► The roles of Ca2 + and cAMP in olfactory transduction were explored using agents which affect calcium channels and second messenger systems. These agents were… (more)

▼ The roles of Ca2 + and cAMP in olfactory transduction were explored using agents which affect calcium channels and second messenger systems. These agents were applied at certain calculated final concentrations onto olfactory epithelia of urethane-anesthetized frogs (Sana PiPlens) by two-sec aerosol spray. During extracellular recording, saturated vapors of isoamyl acetate were delivered every 100 sec in 0.3 sec pulses to produce an electroolfactogram (EOG). Inorganic cations that block inward calcium currents inhibit EOG responses with the following rank order: (La3+) > (Zn2+, Cd2+) > (Al3+, Ca2+, Sr2+) > (Co2+). Application of 7.5 mM La3+ eradicates £0G's, while Ba2+ (which can carry more current that Ca2+) initially produces significant enhancement (F=43.04, p<0.001, df=19). Magnesium ion has no effect on EOG's at 7.5 mM, while 1.5 X 10"4M Ca2+ is significantly inhibitory (F=5.74; p=0.0355; df=12). Control aerosol sprays of distilled water depress EOG's by an average of 5%. The organic calcium channel antagonists diltiazem and verapamil inhibit EOG's by 17% and 36X, respectively, at a concentration of 1.5 X 10~*M. Verapamil produces significant inhibition (F=17.17; p=0.002; df=ll) at 1.5 X 10" 5 M, while the 1,4-dihydropyridine calcium channel antagonists, nicardipine and nifedipine, do not inhibit beyond 1% DMSO controls. Several calmodulin antagonists decrease EOG's, but without correlation to their anti-calmodulin potency. Application of 1.5 X 10"*M chlorpromazine and N-(6-aminohexyl)-5-chloro-l-naphthalenesulfonamide inhibit EOG's by 31% and 27%, respectively, while trifluoperazine inhibits by 23%. Dibutyryl cAMP, a lipophilic mimic of cAMP, produces 54% inhibition at 1.5 X 10" *M. Dibutyryl cGMP, cGMP, cAMP, and adenosine all decrease EOG's by less than 15% compared to distilled water controls. Forskolin, a reversible activator of adenylate cyclase, inhibits EOG's by 57% at 1.5 X 10"5M, which is significant beyond the 1% DMSO controls (F=17.17; p=0.002; df=ll). These data support the hypothesis that Ca2+ participates in olfactory transduction. Calcium ions could serve as charge carriers, second messengers, or both. Cyclic AMP could be involved with the primary excitatory process or sensory adaptation, or both. Advisors/Committee Members: Schafer, Rollie, Orr, Edward, Donahue, Manus J., Gross, Guenter W..

Subjects/Keywords: olfactory senses; calcium ions; intracellular signal transduction; Calcium ions – Physiological effect.; Olfactometry.; Olfactory nerve.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Winegar, B. D. (. D. (1986). Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction. (Thesis). North Texas State University. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc331287/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Winegar, Bruce D (Bruce David). “Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction.” 1986. Thesis, North Texas State University. Accessed January 25, 2021. https://digital.library.unt.edu/ark:/67531/metadc331287/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Winegar, Bruce D (Bruce David). “Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction.” 1986. Web. 25 Jan 2021.

Vancouver:

Winegar BD(D. Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction. [Internet] [Thesis]. North Texas State University; 1986. [cited 2021 Jan 25]. Available from: https://digital.library.unt.edu/ark:/67531/metadc331287/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Winegar BD(D. Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction. [Thesis]. North Texas State University; 1986. Available from: https://digital.library.unt.edu/ark:/67531/metadc331287/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

26. Lee, Michelle. The use of open-face explants to study localized Ca²⁺ signalling during neural induction in Xenopus laevis embryos.

Degree: 2001, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-3874 ; https://doi.org/10.14711/thesis-b714021 ; http://repository.ust.hk/ir/bitstream/1783.1-3874/1/th_redirect.html

► In Xenopus embryos, neural induction results from a specific tissue interaction occurring between the dorsal mesoderm (Spemann's Organizer) and the dorsal ectoderm. The Organizer region… (more)

Subjects/Keywords: Calcium – Physiological effect ; Cellular signal transduction ; Calcium ions ; Calcium-binding proteins ; Gastrulation ; Xenopus – Embryos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, M. (2001). The use of open-face explants to study localized Ca²⁺ signalling during neural induction in Xenopus laevis embryos. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-3874 ; https://doi.org/10.14711/thesis-b714021 ; http://repository.ust.hk/ir/bitstream/1783.1-3874/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Michelle. “The use of open-face explants to study localized Ca²⁺ signalling during neural induction in Xenopus laevis embryos.” 2001. Thesis, Hong Kong University of Science and Technology. Accessed January 25, 2021. http://repository.ust.hk/ir/Record/1783.1-3874 ; https://doi.org/10.14711/thesis-b714021 ; http://repository.ust.hk/ir/bitstream/1783.1-3874/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Michelle. “The use of open-face explants to study localized Ca²⁺ signalling during neural induction in Xenopus laevis embryos.” 2001. Web. 25 Jan 2021.

Vancouver:

Lee M. The use of open-face explants to study localized Ca²⁺ signalling during neural induction in Xenopus laevis embryos. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2001. [cited 2021 Jan 25]. Available from: http://repository.ust.hk/ir/Record/1783.1-3874 ; https://doi.org/10.14711/thesis-b714021 ; http://repository.ust.hk/ir/bitstream/1783.1-3874/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee M. The use of open-face explants to study localized Ca²⁺ signalling during neural induction in Xenopus laevis embryos. [Thesis]. Hong Kong University of Science and Technology; 2001. Available from: http://repository.ust.hk/ir/Record/1783.1-3874 ; https://doi.org/10.14711/thesis-b714021 ; http://repository.ust.hk/ir/bitstream/1783.1-3874/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Anonymous, Lalliansanua. Studies on roles of calcium ions and abscisic acid in modulating detoxification pathways during osmotic stress in seedlings of maize _Zea mays l_; -.

Degree: Botany, 2009, North-Eastern Hill University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/54810

Abstract available newline newline

Summary p. 86-92, Bibliography p. 93-117

Advisors/Committee Members: Chrungoo, N K.

Subjects/Keywords: Calcium Ions; Abscisic Acid; Modulating Detoxification Pathways; Smotic Stress; Seedlings; Maize

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Anonymous, L. (2009). Studies on roles of calcium ions and abscisic acid in modulating detoxification pathways during osmotic stress in seedlings of maize _Zea mays l_; -. (Thesis). North-Eastern Hill University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/54810

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anonymous, Lalliansanua. “Studies on roles of calcium ions and abscisic acid in modulating detoxification pathways during osmotic stress in seedlings of maize _Zea mays l_; -.” 2009. Thesis, North-Eastern Hill University. Accessed January 25, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/54810.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anonymous, Lalliansanua. “Studies on roles of calcium ions and abscisic acid in modulating detoxification pathways during osmotic stress in seedlings of maize _Zea mays l_; -.” 2009. Web. 25 Jan 2021.

Vancouver:

Anonymous L. Studies on roles of calcium ions and abscisic acid in modulating detoxification pathways during osmotic stress in seedlings of maize _Zea mays l_; -. [Internet] [Thesis]. North-Eastern Hill University; 2009. [cited 2021 Jan 25]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/54810.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anonymous L. Studies on roles of calcium ions and abscisic acid in modulating detoxification pathways during osmotic stress in seedlings of maize _Zea mays l_; -. [Thesis]. North-Eastern Hill University; 2009. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/54810

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Candeiro, George Taccio de Miranda. Avaliação da radiopacidade, escoamento, pH e da liberação de íons cálcio de um cimento endodôntico biocerâmico.

Degree: PhD, Endodontia, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/23/23145/tde-15012013-120551/ ;

►

O objetivo do presente trabalho foi avaliar as propriedades físico-químicas de um cimento endodôntico biocerâmico, Endosequence BC Sealer (Brasseler USA, Savannah, GA, EUA). Radiopacidade, escoamento,… (more)

▼

O objetivo do presente trabalho foi avaliar as propriedades físico-químicas de um cimento endodôntico biocerâmico, Endosequence BC Sealer (Brasseler USA, Savannah, GA, EUA). Radiopacidade, escoamento, pH e liberação de íons cálcio (Ca2+) foram analisadas e os resultados foram comparados aos do cimento AH Plus (Dentsply De Trey Gmbh, Konstanz, Alemanha). A radiopacidade e o escoamento foram avaliados de acordo com as normas ISO 6876/2001. Para o teste de radiopacidade, anéis metálicos com 10 mm de diâmetro e 1 mm de altura foram preenchidos com os cimentos. O valor da radiopacidade foi determinado de acordo com a densidade radiográfica, representada em mm Al. A análise do escoamento foi feita com 0,05 mL de cada cimento colocados sobre uma placa de vidro. Outra placa de vidro, com 20 g, foi cuidadosamente colocada sobre o cimento e sobre esta um peso de 100 g, totalizando 120 g, que permaneceram por 7 minutos. Em seguida, foram medidos os diâmetros maiores e menores dos discos formados, sendo esta feita com um paquímetro digital. A liberação de íons cálcio (Ca2+) e o pH foram medidos nos períodos de 3, 24, 72, 168 e 240 horas, com o auxílio de um espectrofotômetro e pHmetro, respectivamente. Os resultados foram analisados estatisticamente pelos testes ANOVA e Tukey, sendo considerado significante quando p<0,05. O cimento biocerâmico analisado apresentou radiopacidade (3,84 mm Al) significantemente menor do que o AH Plus (6,90 mm Al). Foi observado que o cimentos Endosequence BC Sealer e o AH Plus apresentaram escoamentos de 26,96 mm e 21,17 mm, respectivamente (p<0,05). O cimento biocerâmico apresentou maior pH e maior liberação de íons cálcio (Ca2+) do que o cimento AH Plus (p<0,05). Concluiu-se que o cimento Endosequence BC Sealer apresentou valores de radiopacidade e de escoamento em concordância com as norma ISO 6876/2001. Os valores de pH e de liberação de íons cálcio (Ca2+) foram favoráveis para um cimento endodôntico.

The aim of the present study was to evaluate the physicochemical properties of a bioceramic root canal sealer, Endosequence BC Sealer. Radiopacity, pH, release of calcium ions (Ca2+) and flow were analyzed and the results were compared with AH Plus cement. Radiopacity and flow were evaluated according to ISO 6876/2001 standards. For the radiopacity analysis, metallic rings with 10 mm in diameter and 1 mm of thickness were filled with cements. The radiopacity value was determined according to radiographic density (mm Al). The flow test was performed with 0.05 mL of cement placed on a glass plate. A 120-g weight was carefully placed over the cement. The largest and smallest diameters of the disks formed were measured using a digital caliper. The release of Ca2+ and pH were measured at periods of 3, 24, 72, 168 and 240 hours, with spectrophotometer and pHmeter, respectively. Data were analyzed by ANOVA and Tukeys test (p<0.05). The bioceramic endodontic cement showed radiopacity (3.84 mm Al) significantly lower than that of AH Plus (6.90 mm Al). The pH analysis showed that Endosequence BC Sealer…

Advisors/Committee Members: Gavini, Giulio.

Subjects/Keywords: Bioceramic Material; Calcium Ions Release; Escoamento; Flow; Liberação de íons Cálcio; Materiais Biocerâmicos; Materiais Odontológicos; pH; pH; Radiopacidade; Radiopacity; Root Canal Cements

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Candeiro, G. T. d. M. (2012). Avaliação da radiopacidade, escoamento, pH e da liberação de íons cálcio de um cimento endodôntico biocerâmico. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/23/23145/tde-15012013-120551/ ;

Chicago Manual of Style (16^th Edition):

Candeiro, George Taccio de Miranda. “Avaliação da radiopacidade, escoamento, pH e da liberação de íons cálcio de um cimento endodôntico biocerâmico.” 2012. Doctoral Dissertation, University of São Paulo. Accessed January 25, 2021. http://www.teses.usp.br/teses/disponiveis/23/23145/tde-15012013-120551/ ;.

MLA Handbook (7^th Edition):

Candeiro, George Taccio de Miranda. “Avaliação da radiopacidade, escoamento, pH e da liberação de íons cálcio de um cimento endodôntico biocerâmico.” 2012. Web. 25 Jan 2021.

Vancouver:

Candeiro GTdM. Avaliação da radiopacidade, escoamento, pH e da liberação de íons cálcio de um cimento endodôntico biocerâmico. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Jan 25]. Available from: http://www.teses.usp.br/teses/disponiveis/23/23145/tde-15012013-120551/ ;.

Council of Science Editors:

Candeiro GTdM. Avaliação da radiopacidade, escoamento, pH e da liberação de íons cálcio de um cimento endodôntico biocerâmico. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/23/23145/tde-15012013-120551/ ;

29. Schmitt, Emmanuel. Approche performantielle et microstructurale de la durabilité de bétons à base de ciments sulfoalumineux-bélitiques ferriques : Microstructural and performance-based approach of the durability of belite-ye’elimite-ferrite cements based concretes.

Degree: Docteur es, Génie civil, 2014, La Rochelle

URL: http://www.theses.fr/2014LAROS027

►

Les ciments sulfoalumineux (CSA) peuvent être une solution à l’amélioration de l’impact environnemental des bétons, grâce aux faibles consommations énergétiques et émissions de CO2 lors… (more)

▼

Les ciments sulfoalumineux (CSA) peuvent être une solution à l’amélioration de l’impact environnemental des bétons, grâce aux faibles consommations énergétiques et émissions de CO2 lors de leur fabrication. Leurs propriétés expansives, de rapidité de prise et de montée en résistance participent également à l’intérêt grandissant dans les domaines de la construction et de la préfabrication en béton pour ce type de liant. Toutefois, la durabilité des bétons sulfoalumineux reste encore méconnue ou discutée, notamment en milieux acides et face à la corrosion des armatures, en ambiance marine et par carbonatation. Les travaux présentés dans cette thèse se proposent ainsi d’étudier et de comparer la durabilité face à ces attaques, de bétons sulfoalumineux à celle de bétons de référence à base de CEM I et de CEM III/B. Ils s’appuient sur des caractérisations performantielles (indicateurs de durabilité, essais performantiels en conditions accélérées ou naturelles) et microstructurales (phases solides, porosité). Au préalable, l’application des caractérisations sus-citées aux bétons sulfoalumineux est vérifiée et discutée. La bonne résistance des bétons sulfoalumineux aux milieux acides est observée lors d’essais de lixiviation dynamique, à température et pH régulés. L’étude de la pénétration des chlorures (migration en régime permanent et transitoire, isothermes de fixation, exposition en zone de marnage) et de la carbonatation (naturelle et accélérée) de bétons à base de 8 ciments sulfoalumineux de laboratoire différents a permis d’établir des moyens d’améliorer leur durabilité potentielle. Ces moyens ont été appliqués à la fabrication d’un ciment industriel performant, dont la bonne durabilité reste toutefois à confirmer définitivement en conditions réelles.

Calcium sulfoaluminate (CSA) cements can be a solution to improve the environmental impact of concrete, thanks to the lower energy consumption and CO2 emission during their production. Their expansive, fast setting and rapid hardening properties contribute to the growing interest of engineers for concrete construction and prefabrication. However, the durability of sulfoaluminate concretes is not clearly assessed yet, especially in acidic and marine environments, as well as carbonation and chloride induced rebars corrosion. Thus, the aim of this thesis is to study and compare the durability, related to these attacks, of concretes made with CSA cements to ordinary Portland and GGBS cements based reference concretes. This study is founded on microstructural (solid phases, porosity) and performancial (durability indicators, natural and accelerated tests) characterization. Beforehand, the application of these characterizations to CSA concretes is verified and discussed. These show a good resistance to acidic environments when submitted to dynamic leaching tests at pH 3 and 5. The study of chloride ingress (steady and non-steady state migration, binding isotherms and tidal zone exposition) and carbonation (natural and accelerated) in concretes of 8 different laboratory CSA…

Advisors/Committee Members: Ait-Mokhtar, Abdelkarim (thesis director), Amiri, Ouali (thesis director).

Subjects/Keywords: Ciments sulfoalumineux; Durabilité; Approche performantielle; Microstructure; Milieux acides; Carbonatation; Pénétration des ions chlorure; Calcium sulfoaluminate cements; Durability; Performance based approach; Microstructure; Acidic environments; Carbonation; Chloride ingress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schmitt, E. (2014). Approche performantielle et microstructurale de la durabilité de bétons à base de ciments sulfoalumineux-bélitiques ferriques : Microstructural and performance-based approach of the durability of belite-ye’elimite-ferrite cements based concretes. (Doctoral Dissertation). La Rochelle. Retrieved from http://www.theses.fr/2014LAROS027

Chicago Manual of Style (16^th Edition):

Schmitt, Emmanuel. “Approche performantielle et microstructurale de la durabilité de bétons à base de ciments sulfoalumineux-bélitiques ferriques : Microstructural and performance-based approach of the durability of belite-ye’elimite-ferrite cements based concretes.” 2014. Doctoral Dissertation, La Rochelle. Accessed January 25, 2021. http://www.theses.fr/2014LAROS027.

MLA Handbook (7^th Edition):

Schmitt, Emmanuel. “Approche performantielle et microstructurale de la durabilité de bétons à base de ciments sulfoalumineux-bélitiques ferriques : Microstructural and performance-based approach of the durability of belite-ye’elimite-ferrite cements based concretes.” 2014. Web. 25 Jan 2021.

Vancouver:

Schmitt E. Approche performantielle et microstructurale de la durabilité de bétons à base de ciments sulfoalumineux-bélitiques ferriques : Microstructural and performance-based approach of the durability of belite-ye’elimite-ferrite cements based concretes. [Internet] [Doctoral dissertation]. La Rochelle; 2014. [cited 2021 Jan 25]. Available from: http://www.theses.fr/2014LAROS027.

Council of Science Editors:

Schmitt E. Approche performantielle et microstructurale de la durabilité de bétons à base de ciments sulfoalumineux-bélitiques ferriques : Microstructural and performance-based approach of the durability of belite-ye’elimite-ferrite cements based concretes. [Doctoral Dissertation]. La Rochelle; 2014. Available from: http://www.theses.fr/2014LAROS027

University of Oxford

30. Simnett, Sarah Jacqueline. Relaxation processes in cardiac muscle.

Degree: PhD, 1993, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:9b670123-f816-42be-8d74-c37917af200b ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358711

► The technique of laser flash photolysis was used in conjunction with diazo-2 to investigate the relaxation kinetics of skinned trabeculae from the guinea-pig. Diazo-2 is… (more)

▼ The technique of laser flash photolysis was used in conjunction with diazo-2 to investigate the relaxation kinetics of skinned trabeculae from the guinea-pig. Diazo-2 is a caged Ca²⁺ chelator, based on BAPTA, which undergoes a rapid photochemical conversion (> 2000s^-1), when exposed to ultraviolet light, from a Ca²⁺ chelator with low affinity (K_d=2.2μM) to one with a higher affinity (K_d=0.073μM). The resulting rapid Ca²⁺ uptake produces a rapid decline in the free Ca²⁺ concentration (within 2-3msec). Subsequent computer simulations have shown that this rate of Ca 2+ decline is independent of the Ca²⁺ off rate from the Ca²⁺ specific binding site on troponin C. The use of diazo-2 overcomes problems of diffusional delays of Ca²⁺ equilibration which were inherent in previous studies of muscle kinetics. The half time of the relaxation of skinned trabeculae following the photolysis of diazo-2 was 72.3 ± 7.5msec (mean ± SD) at 12°C, and decreased to 53.4±5.2msec at 20°C, both several times faster than the relaxation rate of electrically stimulated trabeculae at the same temperature. The relaxation transients were closely fitted to two exponentials, suggesting that relaxation is governed by two rate limiting processes. Varying the degree of relaxation from 100% relaxation to 30% relaxation had no effect on the half time of the decline in force, indicating that, unlike activation, the processes of relaxation are not cooperative. The effects of the metabolites H⁺, ADP and P_i (at concentrations similar to those reached during cardiac ischaemia and hypoxia) on the rate of relaxation were investigated. P_i increased the relaxation rate, in contrast with its effect in skeletal muscle, where a slowing of relaxation was seen; a discrepancy suggested to be due to differences in the muscle types. Both H⁺ and ADP decreased the relaxation rate; however, their effects on the relaxation transients were distinct, indicating that they altered the kinetics of relaxation by different mechanisms. EMD 57033, a thiadiazinone derivative, is a novel cardiotonic agent which acts by increasing the apparent Ca²⁺ sensitivity of the myofibrils. Maximal Ca²⁺ activated force (P_max) is increased by 10 μM EMD to 138±4.23% of control. EMD 57033 produces a slight increase in the rate of relaxation initiated by the photolysis of diazo-2, unlike the endogenous potentiator ADP. In experiments using caged Ca²⁺ (nitr-5), EMD 57033 markedly increases the rate of activation. This suggests that the Ca²⁺ sensitising effect of EMD 57033 is, at least partially, mediated by an increase in the rate at which crossbridges reach the force-generating state. In contrast, although ADP also increases P_max it decreases the rate of transition out of the force-generating state.

Subjects/Keywords: 612; Myocardium; Heart; Metabolism; Calcium ions; Physiological effect; Contractility (Biology)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Simnett, S. J. (1993). Relaxation processes in cardiac muscle. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:9b670123-f816-42be-8d74-c37917af200b ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358711

Chicago Manual of Style (16^th Edition):

Simnett, Sarah Jacqueline. “Relaxation processes in cardiac muscle.” 1993. Doctoral Dissertation, University of Oxford. Accessed January 25, 2021. http://ora.ox.ac.uk/objects/uuid:9b670123-f816-42be-8d74-c37917af200b ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358711.

MLA Handbook (7^th Edition):

Simnett, Sarah Jacqueline. “Relaxation processes in cardiac muscle.” 1993. Web. 25 Jan 2021.

Vancouver:

Simnett SJ. Relaxation processes in cardiac muscle. [Internet] [Doctoral dissertation]. University of Oxford; 1993. [cited 2021 Jan 25]. Available from: http://ora.ox.ac.uk/objects/uuid:9b670123-f816-42be-8d74-c37917af200b ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358711.

Council of Science Editors:

Simnett SJ. Relaxation processes in cardiac muscle. [Doctoral Dissertation]. University of Oxford; 1993. Available from: http://ora.ox.ac.uk/objects/uuid:9b670123-f816-42be-8d74-c37917af200b ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358711

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