subject:(CD44)

1. 深水, 弥生. 接着分子CD44とFERMドメインの相互作用解析 : Interaction of CD44 cytoplasmic domain and radixin/merlin FERM domain; セッチャクブンシ CD44 ト FERM ドメインノソウゴサヨウカイセキ.

Degree: Nara Institute of Science and Technology / 奈良先端科学技術大学院大学

URL: http://hdl.handle.net/10061/1570

Subjects/Keywords: CD44

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APA (6^th Edition):

深水, �. (n.d.). 接着分子CD44とFERMドメインの相互作用解析 : Interaction of CD44 cytoplasmic domain and radixin/merlin FERM domain; セッチャクブンシ CD44 ト FERM ドメインノソウゴサヨウカイセキ. (Thesis). Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10061/1570

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

深水, 弥生. “接着分子CD44とFERMドメインの相互作用解析 : Interaction of CD44 cytoplasmic domain and radixin/merlin FERM domain; セッチャクブンシ CD44 ト FERM ドメインノソウゴサヨウカイセキ.” Thesis, Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Accessed January 26, 2021. http://hdl.handle.net/10061/1570.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

深水, 弥生. “接着分子CD44とFERMドメインの相互作用解析 : Interaction of CD44 cytoplasmic domain and radixin/merlin FERM domain; セッチャクブンシ CD44 ト FERM ドメインノソウゴサヨウカイセキ.” Web. 26 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

深水 �. 接着分子CD44とFERMドメインの相互作用解析 : Interaction of CD44 cytoplasmic domain and radixin/merlin FERM domain; セッチャクブンシ CD44 ト FERM ドメインノソウゴサヨウカイセキ. [Internet] [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10061/1570.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

深水 �. 接着分子CD44とFERMドメインの相互作用解析 : Interaction of CD44 cytoplasmic domain and radixin/merlin FERM domain; セッチャクブンシ CD44 ト FERM ドメインノソウゴサヨウカイセキ. [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; Available from: http://hdl.handle.net/10061/1570

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

2. Guilbert, Matthieu. Impact de la transactivation des récepteurs membranaires par le (pro)NGF dans les cancers : Impact of (pro)NGF-dependent transactivation of cell membrane receptors in cancers.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2015, Université Lille I – Sciences et Technologies

URL: http://www.theses.fr/2015LIL10097

►

Le laboratoire INSERM U908 a montré que le Nerve Growth Factor (NGF) et son précurseur, le proNGF, sont impliqués dans l’agressivité des tumeurs mammaires via… (more)

▼

Le laboratoire INSERM U908 a montré que le Nerve Growth Factor (NGF) et son précurseur, le proNGF, sont impliqués dans l’agressivité des tumeurs mammaires via des effets sur la croissance, l’angiogenèse ou encore la migration/invasion. Pour autant, aucunes thérapies ciblées n’a à ce jour été approuvée suite à des essais cliniques de traitements visant à inhiber les effets du (pro)NGF et de leurs récepteurs dans les cancers. Ces résultats indiquent que la phosphorylation de TrkA est nécessaire mais pas suffisante pour expliquer le(s) mécanisme(s) par lequel(s) le NGF ou son précurseur participe(nt) au développement tumoral. L’obtention de lignées tumorales résistantes au lestaurtinib est un phénomène rapide, nous avons donc émis l’hypothèse que la signalisation de TrkA indépendante de sa phosphorylation existe de façon « innée » dans les cellules tumorales. Par l’analyse protéomique, nous avons ainsi découvert que le NGF induit le recrutement de CD44 et d’une cascade de signalisation p115RhoGEF/RhoA/ROCK1 (Aubert L*, Guilbert M* et al, Oncotarget, 2015 ; *co-premier auteur). Nous avons ainsi montré que le NGF via le complexe TrkA/CD44 induit la migration et l’invasion des cellules cancéreuses de sein in vivo, et augmente la tumorigénicité in vivo. Quant au proNGF, j’ai pu observer qu’il induit l’internalisation de l’EGFR par la phosphorylation du résidu Y1068. Il en résulte une diminution des effets prolifératif et pro-invasif de l’EGF dans les cellules tumorales. Ces résultats fondamentaux sont tout à fait intéressants même s’ils nécessitent leurs consolidations et doivent permettre de démontrer le caractère pronostic de la détection de TrkA et ses corécepteurs dans le cancer. Ainsi nos études permettront le développement de thérapie ciblée par des firmes pharmaceutiques.

The INSERM U908 unit has showed that Nerve Growth factor (NGF) and its precursor (ProNGF) are implicated in tumor agressivness via their effects on growth, angiogenesis or migration/invasion and metastasis. Nevertheless, (pro)NGF and their receptors targeted therapies failed to demonstrate efficiency and clinical trial are disappointing. These results indicate that TrkA phosphorylation is not sufficient to explain molecular mechanisms of (pro)NGF effects on tumors. Indeed, we obtained lestaurtinib resistant cell lines within 3 weeks of treatment which indicated that resistant mechanisms are innate. So, by functional proteomics analyses, we described that NGF induced the formation of TrkA/CD44 complex and then the recruitment of p115RhoGEF/RhoA/ROCK1 signalling cascade (Aubert L*, Guilbert M* et al, Oncotarget, 2015 ; *equally contributed to this work). We showed that CD44 mediated effects participate to invasive effects of NGF in vitro, and in vivo, we demontrated that CD44 increases NGF induced tumoriginicity. In a second part, I observed that the proNGF regulated EGFR turn over through its phosphorylation on Y1068. This effect on EGFR decreased proliferative and pro-invasive effects of EGF in cancer cells. These fundamental results are…

Advisors/Committee Members: Toillon, Robert-Alain (thesis director), Magné, Nicolas Charles (thesis director).

Subjects/Keywords: ProNGF; Cd44; 571.978

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guilbert, M. (2015). Impact de la transactivation des récepteurs membranaires par le (pro)NGF dans les cancers : Impact of (pro)NGF-dependent transactivation of cell membrane receptors in cancers. (Doctoral Dissertation). Université Lille I – Sciences et Technologies. Retrieved from http://www.theses.fr/2015LIL10097

Chicago Manual of Style (16^th Edition):

Guilbert, Matthieu. “Impact de la transactivation des récepteurs membranaires par le (pro)NGF dans les cancers : Impact of (pro)NGF-dependent transactivation of cell membrane receptors in cancers.” 2015. Doctoral Dissertation, Université Lille I – Sciences et Technologies. Accessed January 26, 2021. http://www.theses.fr/2015LIL10097.

MLA Handbook (7^th Edition):

Guilbert, Matthieu. “Impact de la transactivation des récepteurs membranaires par le (pro)NGF dans les cancers : Impact of (pro)NGF-dependent transactivation of cell membrane receptors in cancers.” 2015. Web. 26 Jan 2021.

Vancouver:

Guilbert M. Impact de la transactivation des récepteurs membranaires par le (pro)NGF dans les cancers : Impact of (pro)NGF-dependent transactivation of cell membrane receptors in cancers. [Internet] [Doctoral dissertation]. Université Lille I – Sciences et Technologies; 2015. [cited 2021 Jan 26]. Available from: http://www.theses.fr/2015LIL10097.

Council of Science Editors:

Guilbert M. Impact de la transactivation des récepteurs membranaires par le (pro)NGF dans les cancers : Impact of (pro)NGF-dependent transactivation of cell membrane receptors in cancers. [Doctoral Dissertation]. Université Lille I – Sciences et Technologies; 2015. Available from: http://www.theses.fr/2015LIL10097

University of Illinois – Chicago

3. Sacks, Joelle Devon. Adhesion and Beyond: CD44 in Ovarian Cancer Spheroids.

Degree: 2018, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/22576

► Epithelial ovarian cancer is a leading cause of death from gynecological malignancies. Challenges in effectively treating patients with metastatic disease and preventing chemo-resistance can be… (more)

▼ Epithelial ovarian cancer is a leading cause of death from gynecological malignancies. Challenges in effectively treating patients with metastatic disease and preventing chemo-resistance can be attributed to the insufficient understanding of the biology and the mechanisms involved in ovarian cancer metastasis. Epithelial ovarian carcinoma (EOC) metastasis is characterized by the shedding of malignant cells from the surface of the primary tumor and their implantation onto the peritoneal surface lining the abdominal cavity in addition to more distant sites. This shedding of malignant cells from the primary site results as either single cells or free-floating multicellular aggregates, known as spheroids, in the ascites. Although single cells and spheroids may potentially seed metastases, considerable evidence now suggests that the aggregation of cells is important for anchorage-independent cell survival and growth, and spheroid formation may represent an important intermediate survival mechanism to facilitate EOC dissemination. This project, thus, aimed to characterize CD44s in EOC spheroids and its functional significance in epithelial ovarian carcinoma metastasis. Recent studies have uncovered human transmembrane cell adhesion molecule CD44 standard (CD44s) isoform expression correlating with high grade and advance-stage ovarian carcinoma. Moreover, it has also been suggested that CD44s may be an important mediator of ovarian cancer cell implantation in the peritoneal cavity enhancing the metastatic potential of ovarian cancer cells. Therefore, we investigated the functional significance of CD44 standard by means of in vitro culture experiments with ovarian cancer spheroids. After characterizing 8 EOC cell lines, a spectrum of CD44 expression was observed and we chose two cell lines with high CD44s (ES-2 and SKOV-3) and two cell lines low in CD44s (OVCAR-4 and OVSAHO) for further testing. Interestingly, the high CD44s-expressing cell lines formed larger spheroids that adhered significantly faster to a monolayer of primary mesothelial cells. Silencing of CD44 using CRISPR/Cas9 in these 2 cell lines reduced spheroid formation suggesting that differential expression of CD44s plays a role in cell-cell adhesion. When we i.p. injected ES-2 CD44-/- cells into athymic nude mice, decreased ascites production and mesentery tumor burden were observed as well as increased overall survival. However, CD44 knockout also significantly increased metastasis to the lung suggesting a suppressive role for CD44 in EOC distant metastasis, as well. This data implies that CD44 enhances the metastatic potential in ovarian cancer spheroids and regulates organ-specific dissemination of ovarian cancer cells, favoring peritoneal dissemination. However, high CD44 expression prevents metastatic dissemination to distant sites, including lungs, thereby limiting its use as a therapeutic target for disease management. Advisors/Committee Members: Barbolina, Maria V (advisor), Tonetti, Debra A (committee member), Jeong, Hyun-Young (committee member), Burdette, Joanna E (committee member), Kajdacsy-Balla, Andre A (committee member), Barbolina, Maria V (chair).

Subjects/Keywords: CD44; ovarian cancer; metastasis; spheroid

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sacks, J. D. (2018). Adhesion and Beyond: CD44 in Ovarian Cancer Spheroids. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/22576

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sacks, Joelle Devon. “Adhesion and Beyond: CD44 in Ovarian Cancer Spheroids.” 2018. Thesis, University of Illinois – Chicago. Accessed January 26, 2021. http://hdl.handle.net/10027/22576.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sacks, Joelle Devon. “Adhesion and Beyond: CD44 in Ovarian Cancer Spheroids.” 2018. Web. 26 Jan 2021.

Vancouver:

Sacks JD. Adhesion and Beyond: CD44 in Ovarian Cancer Spheroids. [Internet] [Thesis]. University of Illinois – Chicago; 2018. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10027/22576.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sacks JD. Adhesion and Beyond: CD44 in Ovarian Cancer Spheroids. [Thesis]. University of Illinois – Chicago; 2018. Available from: http://hdl.handle.net/10027/22576

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

4. Decker, Stacy Ann. A spontaneous murine model for the study of CD44 In glioma progression.

Degree: PhD, Neuroscience, 2012, University of Minnesota

URL: http://purl.umn.edu/136786

► Mouse models of malignant glioma that accurately recapitulate the genetic and phenotypic heterogeneity are essential for advancing brain tumor therapeutics. We have developed a novel… (more)

Subjects/Keywords: CD44; Glioma; Invasion; Tumor development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Decker, S. A. (2012). A spontaneous murine model for the study of CD44 In glioma progression. (Doctoral Dissertation). University of Minnesota. Retrieved from http://purl.umn.edu/136786

Chicago Manual of Style (16^th Edition):

Decker, Stacy Ann. “A spontaneous murine model for the study of CD44 In glioma progression.” 2012. Doctoral Dissertation, University of Minnesota. Accessed January 26, 2021. http://purl.umn.edu/136786.

MLA Handbook (7^th Edition):

Decker, Stacy Ann. “A spontaneous murine model for the study of CD44 In glioma progression.” 2012. Web. 26 Jan 2021.

Vancouver:

Decker SA. A spontaneous murine model for the study of CD44 In glioma progression. [Internet] [Doctoral dissertation]. University of Minnesota; 2012. [cited 2021 Jan 26]. Available from: http://purl.umn.edu/136786.

Council of Science Editors:

Decker SA. A spontaneous murine model for the study of CD44 In glioma progression. [Doctoral Dissertation]. University of Minnesota; 2012. Available from: http://purl.umn.edu/136786

University of Minnesota

5. Klank, Rebecca. Physical Determinants of Glioma Cell Migration and Disease Progression.

Degree: PhD, Biomedical Engineering, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/191324

► Glioblastoma (GBM) is a highly aggressive brain cancer (generally, “glioma”) with poor patient prognosis, even with current standard treatments. In order to rationally develop novel… (more)

▼ Glioblastoma (GBM) is a highly aggressive brain cancer (generally, “glioma”) with poor patient prognosis, even with current standard treatments. In order to rationally develop novel treatments that can significantly extend patient survival, we must first understand at a basic scientific level how the disease progresses. GBM is thought to be fatal due to highly invasive cells that migrate beyond the visible bulk tumor and lead to tumor recurrence after therapeutic intervention. Therefore, we sought to investigate what makes GBM cells invasive at the single-cell level (Chapter 1). Using a genetically induced mouse glioma model and confocal imaging of intact tumor-containing brain slices, we found that, consistent with previous biophysical models, glioma cell migration is biphasic with respect to the concentration of the transmembrane cell adhesion molecule CD44. By contrast, cell proliferation is independent of CD44 level. Additionally, mouse model and human patient survival are also biphasic with respect to CD44 level, with poorest prognosis occurring at intermediate CD44 levels. Thus, migration and survival are both biphasic and are anti-correlated to each other, suggesting that CD44-dependent migration directly affects survival outcomes. We next investigated how these single-cell behaviors impact overall tumor growth and progression (Chapter 2). Noticing that previous models of GBM migration use parameter values for migration rate (defined by a diffusion coefficient, also known as a random motility coefficeint) that are much higher than our measurements of single-cell migration behavior in Chapter 1, a Brownian dynamics (BD) approach was used to simulate single-cell growth, proliferation, and migration, and compare model assumptions. These studies showed that employing the physically-based assumption that tumor cells occupy volume, an assumption not captured in current reaction-diffusion (RD) simulations, resulted in increased tumor spreading behavior with the same input parameters. Specifically, non-overlapping cells can enter a jammed regime where interior cells are subdiffusive, and peripheral cells become biased outward and superdiffusive in a quasi-ballistic expansion. Thus, we show that, when we account for volume conservation, the relatively low values of diffusion coefficient, such as what was measured in Chapter 1, can generate fast progressing tumors that are similar to RD simulations which use diffusion coefficients much greater than what is observed experimentally for single migrating cells. Therefore, we suggest that cellular jamming behavior contributes to the fast spreading of GBM tumors, and that subsequent simulations of GBM growth should incorporate this assumption so that models are physically grounded and achieve consistency between single-cell behavior and bulk tumor progression. Overall, these studies demonstrate the potential importance of fundamental physical effects in driving tumor progression generally, and glioblastoma specifically.

Subjects/Keywords: CD44; Glioblastoma; Glioma; Tumor Modeling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klank, R. (2015). Physical Determinants of Glioma Cell Migration and Disease Progression. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191324

Chicago Manual of Style (16^th Edition):

Klank, Rebecca. “Physical Determinants of Glioma Cell Migration and Disease Progression.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 26, 2021. http://hdl.handle.net/11299/191324.

MLA Handbook (7^th Edition):

Klank, Rebecca. “Physical Determinants of Glioma Cell Migration and Disease Progression.” 2015. Web. 26 Jan 2021.

Vancouver:

Klank R. Physical Determinants of Glioma Cell Migration and Disease Progression. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/11299/191324.

Council of Science Editors:

Klank R. Physical Determinants of Glioma Cell Migration and Disease Progression. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/191324

University of New South Wales

6. Hao, Jingli. Investigating the roles of CD44 and CD147 in prostate cancer metastasis and drug-resistance.

Degree: Clinical School - St George Hospital, 2012, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52038 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10708/SOURCE01?view=true

► Prostate cancer (CaP) is a major health problem in males in western countries. Although early-stage CaP can be controlled using conventional therapies, almost all CaP… (more)

▼ Prostate cancer (CaP) is a major health problem in males in western countries. Although early-stage CaP can be controlled using conventional therapies, almost all CaP patients invariably progress to recurrent castration resistant CaP and eventually die from secondary disease. Targeting tumour-associated antigens is fast emerging as an area of promise for late stage CaP. This thesis provides an overview of literature regarding CaP-related issues, with emphasis on the putative roles of the tumour microenvironment and selected tumour-associated antigens, i.e. CD44 and CD147 in CaP. The aims of the study were to: 1) investigate the expression of CD44 and CD147 in primary CaP tissues and various metastatic CaP cell lines, and identify whether CD44 and CD147 are related with CaP progression, whether they are related with multidrug-resistance proteins, and whether these two proteins are inter-related; 2) develop CaP cell lines with down-regulated CD44 and CD147 expression, study the in vitro functions of these molecules in CaP, and the potential mechanisms involved; and 3) develop CaP animal models with CD44/CD147-knock-down (KD) xenografts, and look at the roles of CD44 and CD147 in vivo in respect of tumour growth, metastasis, and drug resistance.The results indicated that CD44 and CD147 are both associated with CaP progression. CD44 and CD147 are co-expressed and interact in CaP cells. KD of CD44 or CD147 enhanced docetaxel sensitivity, reduced CaP invasive potential, and down-regulated main signal modulators associated with cell growth and survival. In vivo, CD44 or CD147-KD PC-3M-luc subcutaneous xenografts showed suppressed tumour growth with increased docetaxel responsiveness compared to control xenografts. Preliminary studies of an intracardiac model showed metastatic spread of PC-3M-luc cells imaged using bioluminescence, indicating this is an appropriate model for future studies assessing the in vivo roles of CD44 and CD147 in tumour spread. These findings suggest that CD44 and CD147 are both closely related with CaP metastasis and drug resistance. Selective targeting of CD44/CD147 alone or combined with docetaxel may limit CaP metastasis and increase chemosensitivity, indicating promise for future CaP treatment. To date, these studies appear to be the first to show that CD44 and CD147 are collaborators in CaP. Advisors/Committee Members: Li, Yong, Clinical School - St George Hospital, Faculty of Medicine, UNSW, Madigan, Michele, Clinical School - St George Hospital, Faculty of Medicine, UNSW, Khatri, Aparajita, CeramiSphere Pty Ltd.

Subjects/Keywords: CD147; Prostate cancer; CD44

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APA (6^th Edition):

Hao, J. (2012). Investigating the roles of CD44 and CD147 in prostate cancer metastasis and drug-resistance. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52038 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10708/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Hao, Jingli. “Investigating the roles of CD44 and CD147 in prostate cancer metastasis and drug-resistance.” 2012. Doctoral Dissertation, University of New South Wales. Accessed January 26, 2021. http://handle.unsw.edu.au/1959.4/52038 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10708/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Hao, Jingli. “Investigating the roles of CD44 and CD147 in prostate cancer metastasis and drug-resistance.” 2012. Web. 26 Jan 2021.

Vancouver:

Hao J. Investigating the roles of CD44 and CD147 in prostate cancer metastasis and drug-resistance. [Internet] [Doctoral dissertation]. University of New South Wales; 2012. [cited 2021 Jan 26]. Available from: http://handle.unsw.edu.au/1959.4/52038 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10708/SOURCE01?view=true.

Council of Science Editors:

Hao J. Investigating the roles of CD44 and CD147 in prostate cancer metastasis and drug-resistance. [Doctoral Dissertation]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/52038 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10708/SOURCE01?view=true

Ruhr Universität Bochum

7. Notscheid, Nadine Katharina. Befall von Lymphknoten durch Endometriose : Genexpressionsanalyse.

Degree: 2014, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41146

► Problem: Über molekulare Mechanismen der Ausbreitung von Endometriose auf Lymphknoten ist wenig bekannt. Mittels Genexpressionsanalyse sollen daran beteiligte Gene identifiziert werden. Methode: RNA-Extrakiton aus Gewebeproben… (more)

Subjects/Keywords: Endometriose; Lymphknoten; Genexpression; Antigen CD44; Maligne Transformation

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APA (6^th Edition):

Notscheid, N. K. (2014). Befall von Lymphknoten durch Endometriose : Genexpressionsanalyse. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41146

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Notscheid, Nadine Katharina. “Befall von Lymphknoten durch Endometriose : Genexpressionsanalyse.” 2014. Thesis, Ruhr Universität Bochum. Accessed January 26, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41146.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Notscheid, Nadine Katharina. “Befall von Lymphknoten durch Endometriose : Genexpressionsanalyse.” 2014. Web. 26 Jan 2021.

Vancouver:

Notscheid NK. Befall von Lymphknoten durch Endometriose : Genexpressionsanalyse. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Jan 26]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41146.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Notscheid NK. Befall von Lymphknoten durch Endometriose : Genexpressionsanalyse. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-41146

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

8. Mott, Patrick Joseph. Anti-CD44 and Anti-platelet Antibodies have Similar but Distinct Effects in the Treatment of a Mouse Model of Arthritis.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33465

►

Rheumatoid Arthritis (RA) is an autoimmune disease characterized by inflammation and eventual destruction of the synovial joints. The role of platelets in the pathophysiology of… (more)

Subjects/Keywords: Rheumatoid Arthritis; CD44; Platelets; Thrombocytopenia; 0982

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APA (6^th Edition):

Mott, P. J. (2012). Anti-CD44 and Anti-platelet Antibodies have Similar but Distinct Effects in the Treatment of a Mouse Model of Arthritis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33465

Chicago Manual of Style (16^th Edition):

Mott, Patrick Joseph. “Anti-CD44 and Anti-platelet Antibodies have Similar but Distinct Effects in the Treatment of a Mouse Model of Arthritis.” 2012. Masters Thesis, University of Toronto. Accessed January 26, 2021. http://hdl.handle.net/1807/33465.

MLA Handbook (7^th Edition):

Mott, Patrick Joseph. “Anti-CD44 and Anti-platelet Antibodies have Similar but Distinct Effects in the Treatment of a Mouse Model of Arthritis.” 2012. Web. 26 Jan 2021.

Vancouver:

Mott PJ. Anti-CD44 and Anti-platelet Antibodies have Similar but Distinct Effects in the Treatment of a Mouse Model of Arthritis. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/1807/33465.

Council of Science Editors:

Mott PJ. Anti-CD44 and Anti-platelet Antibodies have Similar but Distinct Effects in the Treatment of a Mouse Model of Arthritis. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33465

9. Κωνσταντόπουλος, Κωνσταντίνος. Έκφραση και ρόλος των πρωτεογλυκανών CD44 και versican κατά την ανάπτυξη του πρώιμου εμβρύου.

Degree: 2011, University of Patras

URL: http://hdl.handle.net/10889/5641

► Οι πρωτεογλυκάνες και οι αλυσίδες γλυκοζαμινογλυκανών τους αλληλεπιδρούν με αυξητικούς παράγοντες, διαμεμβρανικούς υποδοχείς όπως οι ιντεγκρίνες, ένζυμα, αναστολείς πρωτεασών και με άλλα μόρια της εξωκυττάριας… (more)

▼ Οι πρωτεογλυκάνες και οι αλυσίδες γλυκοζαμινογλυκανών τους αλληλεπιδρούν με αυξητικούς παράγοντες, διαμεμβρανικούς υποδοχείς όπως οι ιντεγκρίνες, ένζυμα, αναστολείς πρωτεασών και με άλλα μόρια της εξωκυττάριας ύλης όπως η ινονεκτίνη, η λαμινίνη και η tenascin. Στην παρούσα διατριβή μελετήσαμε τη χωροχρονική κατανομή των πρωτεογλυκανών versican και CD44 με τη μέθοδο της RT-PCR και του ανοσοφθορισμού από το στάδιο ΧΙ-ΧΙΙ (μορίδιο) έως το στάδιο ΗΗ16+ (28-29 ζεύγη σωμιτών) και τον ρόλο της versican και της CD44 με τη χρήση μονοκλωνικών αντισωμάτων έναντι αυτών κατά την ανάπτυξη του πρώιμου εμβρύου. Η versican είναι πρωτεογλυκάνη θειικής χονδροϊτίνης και αλληλεπιδρά με αυξητικούς παράγοντες, με διάφορες πρωτεΐνες της εξωκυττάριας ύλης και με διαμεμβρανικούς υποδοχείς όπως η CD44. Τα αποτελέσματα της RT-PCR έδειξαν ότι το mRNA της versican εκφράζεται σε όλα τα αναπτυξιακά στάδια που μελετήσαμε. Ενδιαφέρον παρουσιάζουν τα προϊόντα εναλλακτικής ωρίμανσης της versican που ανιχνεύσαμε ακόμα και στο στάδιο του μοριδίου και που η έκφρασή τους ρυθμίζεται αναπτυξιακά. Η παρουσία του mRNA της versican σε υψηλά επίπεδα στο στάδιο του μοριδίου (ΧΙ-ΧΙΙ) υποδεικνύει ότι το mRNA της versican είναι ωογενετικής προέλευσης στο στάδιο αυτό. Τα πειράματα μας του ανοσοφθορισμού έδειξαν ότι η versican πρωτεΐνη ανιχνεύεται στο στάδιο του μοριδίου και εκφράζεται έντονα στην επιβλάστη και στην υποβλάστη στο στάδιο του προχωρημένου βλαστιδίου (στάδιο ΧΙΙΙ). Στο στάδιο ΗΗ3+ (ενδιάμεσο γαστρίδιο / intermediate streak) παρατηρήσαμε μεγάλη ένταση φθορισμού στα κύτταρα που μεταναστεύουν μέσα από την πρωτογενή αύλακα και στα μεσεγχυματικά κύτταρα που θα σχηματίσουν το μεσόδερμα και το ενδόδερμα. Στο στάδιο ΗΗ4 (προχωρημένο γαστρίδιο / definitive streak) ένταση φθορισμού της versican ανιχνεύθηκε κύτταρα που μεταναστεύουν μέσα από την πρωτογενή αύλακα καθώς και στα κύτταρα που έχουν αρχίσει να σχηματίζουν το μεσόδερμα και το ενδόδερμα. Στο στάδιο που το έμβρυο έχει σχηματίσει 4 ζεύγη σωμιτών (στάδιο ΗΗ8), ανιχνεύσαμε υψηλή ένταση φθορισμού της versican στη νευρική πλάκα και στις νευρικές πτυχές καθώς ανασηκώνονται να σχηματίσουν το νευρικό σωλήνα. Στο στάδιο ΗΗ12 (16 ζεύγη σωμιτών), ισχυρή ένταση φθορισμού της versican ανιχνεύσαμε στο νευρικό σωλήνα, στο γειτονικό του εξώδερμα, στα κύτταρα της νευρικής ακρολοφίας (neural crest), στα κύτταρα του σωμίτη, στο μεσονέφρο και στο γειτονικό του πλάγιο μεσόδερμα που θα σχηματίσει τους μεσονεφρικούς σωληνίσκους. Αργότερα στην ανάπτυξη, ισχυρή ένταση φθορισμού της versican ανιχνεύσαμε επίσης στο διεγκέφαλο, στον οπτικό μίσχο, στο μεσεγκέφαλο, στο μυελεγκέφαλο, στα τοιχώματα του φάρυγγα και της ραχιαίας αορτής, στο ραχιαίο μεσοκάρδιο, στο μυοκάρδιο και στο ενδοκάρδιο, στο μυοτόμο και σκληροτόμο στους σωμίτες, στα τοιχώματα του εντέρου, καθώς και στην εξωκυττάρια ύλη των εμβρυϊκών κοιλοτήτων. Πειράματα σε έμβρυα που εκτέθηκαν στο αντίσωμα έναντι της versican σε διαφορετικά στάδια ανάπτυξης από το μορίδιο ως το προχωρημένο γαστρίδιο έδειξαν ότι η versican πιθανόν να συμμετέχει στα μονοπάτια σηματοδότησης που… Advisors/Committee Members: Ζάγκρης, Νικόλας, Konstantopoulos, Konstantinos, Γιομπρές, Παναγιώτης, Κίλιας, Γεώργιος, Δερμών, Αικατερίνη, Δημητριάδης, Γεώργιος, Συνετός, Διονύσιος, Φλωρδέλλης, Χριστόδουλος.

Subjects/Keywords: Πρωτεογλυκάνες; Υαλουρονικό; 571.86; Proteoglycans; Hyaluronan; Versican; CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Κωνσταντόπουλος, �. (2011). Έκφραση και ρόλος των πρωτεογλυκανών CD44 και versican κατά την ανάπτυξη του πρώιμου εμβρύου. (Doctoral Dissertation). University of Patras. Retrieved from http://hdl.handle.net/10889/5641

Chicago Manual of Style (16^th Edition):

Κωνσταντόπουλος, Κωνσταντίνος. “Έκφραση και ρόλος των πρωτεογλυκανών CD44 και versican κατά την ανάπτυξη του πρώιμου εμβρύου.” 2011. Doctoral Dissertation, University of Patras. Accessed January 26, 2021. http://hdl.handle.net/10889/5641.

MLA Handbook (7^th Edition):

Κωνσταντόπουλος, Κωνσταντίνος. “Έκφραση και ρόλος των πρωτεογλυκανών CD44 και versican κατά την ανάπτυξη του πρώιμου εμβρύου.” 2011. Web. 26 Jan 2021.

Vancouver:

Κωνσταντόπουλος �. Έκφραση και ρόλος των πρωτεογλυκανών CD44 και versican κατά την ανάπτυξη του πρώιμου εμβρύου. [Internet] [Doctoral dissertation]. University of Patras; 2011. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10889/5641.

Council of Science Editors:

Κωνσταντόπουλος �. Έκφραση και ρόλος των πρωτεογλυκανών CD44 και versican κατά την ανάπτυξη του πρώιμου εμβρύου. [Doctoral Dissertation]. University of Patras; 2011. Available from: http://hdl.handle.net/10889/5641

10. Perrot, Gwen. Identification d’un nouveau partenaire de LRP1 : CD44, le récepteur de l’acide hyaluronique : Identification of a new partner for LRP1 : CD44, the hyaluronan receptor.

Degree: Docteur es, Biochimie et Biologie Cellulaire, 2012, Reims

URL: http://www.theses.fr/2012REIMS006

►

LRP1 est un récepteur d’endocytose multifonctionnel capable non seulement d’interagir avec de nombreux ligands et de réguler leur endocytose, mais également de moduler certaines voies… (more)

▼

LRP1 est un récepteur d’endocytose multifonctionnel capable non seulement d’interagir avec de nombreux ligands et de réguler leur endocytose, mais également de moduler certaines voies de signalisation intracellulaire. Ce récepteur se révèle implique dans de nombreux mécanismes physiologiques et pathologiques, comme l’athérosclérose, la maladie d’Alzheimer ou encore le cancer. La multiplicité de ses partenaires fait émerger le concept selon lequel LRP1 est capable de réguler le protéome membranaire et ainsi d’influencer le comportement migratoire de nombreuses cellules. De plus, les résultats récents obtenus au sein de notre laboratoire indiquent que ce récepteur d’endocytose est capable de contrôler la dynamique d’adhérence des cellules tumorales. Nous avons donc cherche à identifier un nouveau partenaire membranaire de LRP1, capable de participer à la régulation de l’adhérence tumorale. Notre étude s’est portée sur le récepteur d’adhérence CD44. L’utilisation de RAP, un antagoniste de LRP1 révèle que ce récepteur d’endocytose module la présence de CD44 à la membrane plasmique. Nous avons également découvert que ces deux récepteurs colocalisent fortement dans la cellule tumorale, que ce soit à la surface cellulaire ou au niveau intracellulaire. De plus LRP1 et CD44 co-immunoprécipitent à partir d’extraits totaux et membranaires, indiquant que ces deux récepteurs sont fortement associés au sein d’un même complexe moléculaire. L’étude de la répartition membranaire du complexe LRP1/CD44 a montré qu’il était en grande partie situé dans les radeaux lipidiques et particulièrement les cavéoles. Toutefois ces structures n’apparaissent pas nécessaires à l’établissement du complexe et ne sont pas impliquées dans les processus d’endocytose relatifs à ces récepteurs. En effet, des expérimentations visant à quantifier l’endocytose de CD44 couplées à différents traitements (β-MCD, conditions hyperosmotiques) révèlent que l’endocytose de ce complexe implique principalement la voie des vésicules tapissées de clathrine. Nous avons également démontré que ce mécanisme dynamique permettait de réguler l’adhérence tumorale. Enfin, des travaux sur le shedding de CD44 ont permis d’identifier les protéases impliquées (MT1MMP, ADAMs 10 et 17) et mettent en évidence un effet protecteur de LRP1 sur le clivage de l’ectodomaine de CD44.

The low-density lipoprotein receptor-related protein-1(LRP-1) is a large endocytic recept or mediating the clearance of various molecules from the extracellular matrix. In the field of cancer, LRP1-metiated endocytosis was first associated to anti-properties. However, recent results suggested that LRP-1 may coordinate the adhesion-deadhesion balance in malignant cells to support tumor progression. Here, we observed that LRP-1-silencing or RAP (receptor-associated protein) treatment led to tumor cell surface accumulation of CD44. Moreover, we evidenced a tight interaction between CD44 and LRP-1, not exclusively localized in lipid rafts. Overexpression of LRP-1-derived mini-receptors indicated that the fourth…

Advisors/Committee Members: Martiny, Laurent (thesis director).

Subjects/Keywords: LRP1; CD44; Endocytose; Adhérence; Cander; Radeaux lipidiques

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Perrot, G. (2012). Identification d’un nouveau partenaire de LRP1 : CD44, le récepteur de l’acide hyaluronique : Identification of a new partner for LRP1 : CD44, the hyaluronan receptor. (Doctoral Dissertation). Reims. Retrieved from http://www.theses.fr/2012REIMS006

Chicago Manual of Style (16^th Edition):

Perrot, Gwen. “Identification d’un nouveau partenaire de LRP1 : CD44, le récepteur de l’acide hyaluronique : Identification of a new partner for LRP1 : CD44, the hyaluronan receptor.” 2012. Doctoral Dissertation, Reims. Accessed January 26, 2021. http://www.theses.fr/2012REIMS006.

MLA Handbook (7^th Edition):

Perrot, Gwen. “Identification d’un nouveau partenaire de LRP1 : CD44, le récepteur de l’acide hyaluronique : Identification of a new partner for LRP1 : CD44, the hyaluronan receptor.” 2012. Web. 26 Jan 2021.

Vancouver:

Perrot G. Identification d’un nouveau partenaire de LRP1 : CD44, le récepteur de l’acide hyaluronique : Identification of a new partner for LRP1 : CD44, the hyaluronan receptor. [Internet] [Doctoral dissertation]. Reims; 2012. [cited 2021 Jan 26]. Available from: http://www.theses.fr/2012REIMS006.

Council of Science Editors:

Perrot G. Identification d’un nouveau partenaire de LRP1 : CD44, le récepteur de l’acide hyaluronique : Identification of a new partner for LRP1 : CD44, the hyaluronan receptor. [Doctoral Dissertation]. Reims; 2012. Available from: http://www.theses.fr/2012REIMS006

King Abdullah University of Science and Technology

11. Alsharif, Nouf. Towards The Generation of Functionalized Magnetic Nanowires to Target Leukemic Cells.

Degree: 2016, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/607655

► In recent years, magnetic nanowires (NWs) have been widely used for their therapeutic potential in biomedical applications. The use of iron (Fe) NWs combines two… (more)

▼ In recent years, magnetic nanowires (NWs) have been widely used for their therapeutic potential in biomedical applications. The use of iron (Fe) NWs combines two important properties, biocompatibility and remote manipulation by magnetic fields. In addition the NWs can be coated and functionalized to target cells of interest and, upon exposure to an alternating magnetic field, have been shown to induce cell death on several types of adherent cells, including several cancer cell types. For suspension cells, however, using these NWs has been much less effective primarily due to the free-floating nature of the cells minimizing the interaction between them and the NWs. Leukemic cells express higher levels of the cell surface marker CD44 (Braumüller, Gansauge, Ramadani, & Gansauge, 2000), compared to normal blood cells. The goal of this study was to functionalize Fe NWs with a specific monoclonal antibody towards CD44 in order to target leukemic cells (HL-60 cells). This approach is expected to increase the probability of a specific binding to occur between HL-60 cells and Fe NWs. Fe NWs were fabricated with an average diameter of 30-40 nm and a length around 3-4 μm. Then, they were coated with both 3-Aminopropyl-triethoxysilane and bovine serum albumin (BSA) in order to conjugate them with an anti-CD44 antibody (i.e. anti-CD44-iron NWs). The antibody interacts with the amine group in the BSA via the 1-Ethyl-3-3-dimethylaminopropyl-carbodiimide and N-Hydroxysuccinimide coupling. The NWs functionalization was confirmed using a number of approaches including: infrared spectroscopy, Nanodrop to measure the concentration of CD44 antibody, as well as fluorescent-labeled secondary antibody staining to detect the primary CD44 antibody. To confirm that the anti-CD44-iron NWs and bare Fe NWs, in the absence of a magnetic field, were not toxic to HL-60 cells, cytotoxicity assays using XTT (2,3-Bis-2-Methoxy-4-Nitro-5-Sulfophenyl-2H-Tetrazolium-5-Carboxanilide) were performed and resulted in a high level of biocompatibility. In addition, the internalization of the coated NWs have been studied by coating them with a pH dependent dye (pHrodoTM Red) that showed a signal once the NWs were internalized by the cell.

Subjects/Keywords: Iron; Nanowires; Biofunctionalization; CD44; Leukemia; HL60

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alsharif, N. (2016). Towards The Generation of Functionalized Magnetic Nanowires to Target Leukemic Cells. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/607655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alsharif, Nouf. “Towards The Generation of Functionalized Magnetic Nanowires to Target Leukemic Cells.” 2016. Thesis, King Abdullah University of Science and Technology. Accessed January 26, 2021. http://hdl.handle.net/10754/607655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alsharif, Nouf. “Towards The Generation of Functionalized Magnetic Nanowires to Target Leukemic Cells.” 2016. Web. 26 Jan 2021.

Vancouver:

Alsharif N. Towards The Generation of Functionalized Magnetic Nanowires to Target Leukemic Cells. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2016. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10754/607655.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alsharif N. Towards The Generation of Functionalized Magnetic Nanowires to Target Leukemic Cells. [Thesis]. King Abdullah University of Science and Technology; 2016. Available from: http://hdl.handle.net/10754/607655

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. 永井, 伯弥. Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.

Degree: 博士(歯学), 歯学, 2019, Hokkaido University

URL: http://hdl.handle.net/2115/77060

► Osteoblastic differentiation into osteocytes is regulated in a temporospatial manner. It is difficult to identify which of osteoblasts are at the verge of differentiating into… (more)

▼ Osteoblastic differentiation into osteocytes is regulated in a temporospatial manner. It is difficult to identify which of osteoblasts are at the verge of differentiating into osteocytes; however, podoplanin reportedly localizes to osteoblasts/osteocytes at the verge of differentiating or right after embedding in the bone matrix. In this study, therefore, we attempted to examine the biological function of podoplanin for osteoblastic differentiation into osteocytes. Podoplanin-reactive osteoblasts show dynamic rearrangement of actin filament distribution and show their assembly along the cell membranes and the basal region of the cytoplasmic processes, whereas osteoblasts with little podoplanin immunoreactivity revealed well-developed stress fibers inside the cells. In the metaphysis (the bone remodeling area), CD44-positive osteoclasts were close to or in contact with podoplanin-reactive osteoblasts showing immunoreactivity of phosphorylated ezrin, an ERM family member associated with actin filament arrangement. Therefore, CD44-positive osteoclasts may participate in the differentiation of podoplanin-positive/phosphorylated ezrin-reactive osteoblasts into osteocytes. Immunoblotting analysis using calvariae from neonatal mice demonstrated increased phosphorylated ezrin when exogenous CD44 was administered. On the endosteal surfaces of cortical bones (a modeling area), bone podoplanin-positive osteoblasts were uniformly localized at certain intervals. Although there was no contact with CD44-positive cells, several osteoblasts showed the immunoreactivities of podoplanin and phosphorylated ezrin. Therefore, unlike femoral trabecules, molecules other than CD44 may transduce the podoplanin/EMR family signaling in preparation for osteocytic differentiation in the cortical bone. Taken together, the interplay between podoplanin and CD44 appears to be, at least in part, associated with ezrin phosphorylation and the subsequent actin rearrangement as well as the facilitation of osteoblastic differentiation into osteocytes in metaphyseal trabecules; however, it does not seem to be associated with the cortical bone. Advisors/Committee Members: 横山, 敦郎, 網塚, 憲生, 土門, 卓文, 長谷川, 智香.

Subjects/Keywords: osteoblast; osteocyte; podoplanin; EMR family; CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

永井, �. (2019). Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone. (Doctoral Dissertation). Hokkaido University. Retrieved from http://hdl.handle.net/2115/77060

Chicago Manual of Style (16^th Edition):

永井, 伯弥. “Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.” 2019. Doctoral Dissertation, Hokkaido University. Accessed January 26, 2021. http://hdl.handle.net/2115/77060.

MLA Handbook (7^th Edition):

永井, 伯弥. “Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone.” 2019. Web. 26 Jan 2021.

Vancouver:

永井 �. Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone. [Internet] [Doctoral dissertation]. Hokkaido University; 2019. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/2115/77060.

Council of Science Editors:

永井 �. Immunocytochemical assessment of cell differentiation of podoplanin-positive osteoblasts into osteocytes in murine bone. [Doctoral Dissertation]. Hokkaido University; 2019. Available from: http://hdl.handle.net/2115/77060

University of South Florida

13. Gebhard, Anthony. CD44 containing complexes as a therapeutic target in Multiple Myeloma.

Degree: 2013, University of South Florida

URL: https://scholarcommons.usf.edu/etd/4815

► Our laboratory recently reported that treatment with the d-amino acid containing peptide HYD1 induces necrotic cell death in multiple myeloma (MM) cell lines. Due to… (more)

▼ Our laboratory recently reported that treatment with the d-amino acid containing peptide HYD1 induces necrotic cell death in multiple myeloma (MM) cell lines. Due to the intriguing biological activity and promising in vivo activity of HYD1, we pursued strategies for increasing the therapeutic efficacy of the parent linear peptide. These efforts led to the development of a cyclized peptidomimetic, MTI-101, with increased in vitro activity and robust in vivo activity as a single agent using two myeloma models that consider the bone marrow microenvironment. MTI-101 treatment resulted in mechanistically similar hallmarks of HYD1 induced cell death, namely the generation of ROS, depletion of ATP levels, and failure to activate caspase-3. Moreover, MTI-101 was shown to be cross-resistant in the HYD1 acquired resistant H929-60 cell line that was previously developed in our laboratory. In the present study, we pursued an unbiased chemical biology approach using biotinylated peptide affinity purification and LC-MS/MS analysis to identify binding partners of MTI-101. Using this approach, CD44 was identified as a predominant binding partner. Using an ELISA based assay, we showed that biotinylated peptide bound to full length recombinant human CD44 in a concentration dependent manner. Reducing the cell surface expression of CD44 was accompanied by the activation of caspase-3 and cell death was observed in the NCI-H929 and U266 MM cell lines, indicating that MM cells require CD44 expression for survival. Ectopic expression of CD44s correlated with increased binding of the FAM-conjugated peptide in the 8226 MM cell line, and this was further corroborated using CD44 knockout mice which also showed less peptide binding compared to wild-type. However, ectopic expression of CD44s was not sufficient to increase the sensitivity to MTI-101 induced cell death. Mechanistically, we show that MTI-101 induced a pro-survival signal through the activation of Erk1/2 and that CD44 formed a complex with Pyk2. These data corroborate with that of which was previously observed with the parental peptide being a partial agonist and inducing an autophagic survival signal. With respect to cell death, we showed that CD44 forms a complex with known death inducing proteins caspase-8, caspase-10, Rip1, Rip3, Drp1, TNFAIP8, and PGAM5. Furthermore, we demonstrated that MTI-101 induced mitochondrial fission which may be modulated by a Rip1, Rip3 or Drp1 dependent and independent pathway. Finally, we show that MTI-101 has robust activity as a single agent in the SCID-Hu bone implant and 5TGM1 in vivo model of multiple myeloma. Together these data continue to support the further development of this class of compounds as well as identify CD44 as a therapeutic target for the treatment of MM.

Subjects/Keywords: CD44; HYD1; MTI-101; Necroptosis; Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gebhard, A. (2013). CD44 containing complexes as a therapeutic target in Multiple Myeloma. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/4815

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gebhard, Anthony. “CD44 containing complexes as a therapeutic target in Multiple Myeloma.” 2013. Thesis, University of South Florida. Accessed January 26, 2021. https://scholarcommons.usf.edu/etd/4815.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gebhard, Anthony. “CD44 containing complexes as a therapeutic target in Multiple Myeloma.” 2013. Web. 26 Jan 2021.

Vancouver:

Gebhard A. CD44 containing complexes as a therapeutic target in Multiple Myeloma. [Internet] [Thesis]. University of South Florida; 2013. [cited 2021 Jan 26]. Available from: https://scholarcommons.usf.edu/etd/4815.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gebhard A. CD44 containing complexes as a therapeutic target in Multiple Myeloma. [Thesis]. University of South Florida; 2013. Available from: https://scholarcommons.usf.edu/etd/4815

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

King Abdullah University of Science and Technology

14. Darwish, Manar M. Role of the Phosphorylation of mTOR in the Differentiation of AML Cells Triggered with CD44 Antigen.

Degree: 2013, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/293659

► Acute myeloid leukemia (AML) is a hematological disorder characterized by blockage of differentiation of myeloblasts. To date, the main therapy for AML is chemotherapy. Yet,… (more)

Subjects/Keywords: mTOR; Phosphorylation; Differentiation; AML; CD44; Antigen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Darwish, M. M. (2013). Role of the Phosphorylation of mTOR in the Differentiation of AML Cells Triggered with CD44 Antigen. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/293659

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Darwish, Manar M. “Role of the Phosphorylation of mTOR in the Differentiation of AML Cells Triggered with CD44 Antigen.” 2013. Thesis, King Abdullah University of Science and Technology. Accessed January 26, 2021. http://hdl.handle.net/10754/293659.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Darwish, Manar M. “Role of the Phosphorylation of mTOR in the Differentiation of AML Cells Triggered with CD44 Antigen.” 2013. Web. 26 Jan 2021.

Vancouver:

Darwish MM. Role of the Phosphorylation of mTOR in the Differentiation of AML Cells Triggered with CD44 Antigen. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2013. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10754/293659.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Darwish MM. Role of the Phosphorylation of mTOR in the Differentiation of AML Cells Triggered with CD44 Antigen. [Thesis]. King Abdullah University of Science and Technology; 2013. Available from: http://hdl.handle.net/10754/293659

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

King Abdullah University of Science and Technology

15. Alghuneim, Arwa. Evaluating the regulation of signaling pathways downstream of CD44 antibody treatment in AML.

Degree: 2019, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/656305

► Acute myeloid leukemia (AML) is a subset of leukemia that is characterized by the clonal expansion of cytogenetically and molecularly abnormal myeloid blasts. These blasts… (more)

Subjects/Keywords: Acute Myeloid Leukemia; Epigenetic; Differentiation; Anti-CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alghuneim, A. (2019). Evaluating the regulation of signaling pathways downstream of CD44 antibody treatment in AML. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/656305

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alghuneim, Arwa. “Evaluating the regulation of signaling pathways downstream of CD44 antibody treatment in AML.” 2019. Thesis, King Abdullah University of Science and Technology. Accessed January 26, 2021. http://hdl.handle.net/10754/656305.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alghuneim, Arwa. “Evaluating the regulation of signaling pathways downstream of CD44 antibody treatment in AML.” 2019. Web. 26 Jan 2021.

Vancouver:

Alghuneim A. Evaluating the regulation of signaling pathways downstream of CD44 antibody treatment in AML. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2019. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10754/656305.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alghuneim A. Evaluating the regulation of signaling pathways downstream of CD44 antibody treatment in AML. [Thesis]. King Abdullah University of Science and Technology; 2019. Available from: http://hdl.handle.net/10754/656305

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

16. Leite Nascimento, Thais. Lipoplexes recouverts d’acide hyaluronique pour le ciblage d’ARN interférant à des cellules tumorales surexprimant le récepteur CD44 : Hyaluronic acid-coated lipoplexes for siRNA targeting to tumor cells overexpressing CD44 receptors.

Degree: Docteur es, Pharmacotechnie et biopharmacie, 2015, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2015PA114834

► Des progrès récents dans l’utilisation préclinique et clinique des petits ARN interférents (siRNA) ont montré leur potentiel en tant qu’inhibiteur de la synthèse protéique dans… (more)

▼ Des progrès récents dans l’utilisation préclinique et clinique des petits ARN interférents (siRNA) ont montré leur potentiel en tant qu’inhibiteur de la synthèse protéique dans de nombreuses pathologies comme le cancer. L’administration des siRNA rencontre un certain nombre de problèmes liés à leur dégradation rapide dans les milieux biologiques, ainsi qu’à leur difficulté à pénétrer au sein des cellules cibles en raison de leur hydrophilie et de leur charge négative. Une des clés de l’amélioration de l’efficacité thérapeutique de ces molécules repose sur l’emploi de vecteurs. Au cours de cette thèse, des lipoplexes capables de protéger les siRNA contre la dégradation et de favoriser leur transport jusqu’aux cellules cibles ont été développés et optimisés. Pour ce faire, des lipoplexes recouverts d’HA ont été formulés pour la vectorisation active de siRNA vers des cellules tumorales surexprimant le récepteur CD44. Dans la première partie de cette thèse, la formation des lipoplexes a été étudiée, ainsi que les paramètres influençant leur organisation supramoléculaire. L'insertion de l'HA dans la structure du liposome au moment de la formation des vésicules a entraîné une augmentation de la taille des liposomes en fonction de la concentration d’HA. Leur complexation avec les siRNA a encore augmenté la taille des particules obtenues. L’ajout des acides nucléiques lors de la formation des lipoplexes a provoqué un déplacement d'une partie du conjugué HA-DOPE de la structure des lipoplexes, comme montré par électrophorèse capillaire. La titration calorimétrique isotherme et les études de diffraction des rayons X ont démontré que, sous l’effet des interactions électrostatiques avec les siRNA, un réarrangement des bicouches lipidiques a lieu, conduisant à la formation de vésicules oligolamellaires, confirmé visuellement par cryo-microscopie. Enfin, le positionnement convenable de l’HA sur la surface des lipoplexes et sa capacité de se lier spécifiquement aux récepteurs de CD44 ont été démontrés par la technique de résonance plasmonique de surface. Dans la deuxième partie de cette thèse, la capture cellulaire et localisation intracellulaire des lipoplexes-HA ont été évalués par cytométrie en flux et microscopie de fluorescence, et ont montré que les lipoplexes modifies par l’HA sont internalisés plus rapidement que les lipoplexes non modifiés, et une fois dans des cellules, ils sont localisés principalement à l’intérieur des endosomes. La capacité des lipoplexes à transporter des molécules de siRNA intactes au cytoplasme a été confirmée par 81 % d'inhibition d'expression de luciferase in vitro sur la lignée cellulaire de cancer du poumon A549-luc. In vivo, le traitement avec les lipoplexes-HA transportant un siRNA anti-luciferase a mené à une diminution statistiquement significative de l’expression de luciferase, ce qui a été confirmé par la réduction de l'expression d’ARNm de la luciferase dans le poumon des animaux traités avec les lipoplexes-HA. L'analyse de la distribution des lipoplexes dans les poumons a montré que les… Advisors/Committee Members: Fattal, Elias (thesis director).

Subjects/Keywords: Lipoplexes; ARN interférents; CD44; A549; Vectorisation; Lipoplexes; SiRNA; Hyaluronic acid; CD44; A549; Targeting

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leite Nascimento, T. (2015). Lipoplexes recouverts d’acide hyaluronique pour le ciblage d’ARN interférant à des cellules tumorales surexprimant le récepteur CD44 : Hyaluronic acid-coated lipoplexes for siRNA targeting to tumor cells overexpressing CD44 receptors. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2015PA114834

Chicago Manual of Style (16^th Edition):

Leite Nascimento, Thais. “Lipoplexes recouverts d’acide hyaluronique pour le ciblage d’ARN interférant à des cellules tumorales surexprimant le récepteur CD44 : Hyaluronic acid-coated lipoplexes for siRNA targeting to tumor cells overexpressing CD44 receptors.” 2015. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 26, 2021. http://www.theses.fr/2015PA114834.

MLA Handbook (7^th Edition):

Leite Nascimento, Thais. “Lipoplexes recouverts d’acide hyaluronique pour le ciblage d’ARN interférant à des cellules tumorales surexprimant le récepteur CD44 : Hyaluronic acid-coated lipoplexes for siRNA targeting to tumor cells overexpressing CD44 receptors.” 2015. Web. 26 Jan 2021.

Vancouver:

Leite Nascimento T. Lipoplexes recouverts d’acide hyaluronique pour le ciblage d’ARN interférant à des cellules tumorales surexprimant le récepteur CD44 : Hyaluronic acid-coated lipoplexes for siRNA targeting to tumor cells overexpressing CD44 receptors. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2015. [cited 2021 Jan 26]. Available from: http://www.theses.fr/2015PA114834.

Council of Science Editors:

Leite Nascimento T. Lipoplexes recouverts d’acide hyaluronique pour le ciblage d’ARN interférant à des cellules tumorales surexprimant le récepteur CD44 : Hyaluronic acid-coated lipoplexes for siRNA targeting to tumor cells overexpressing CD44 receptors. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2015. Available from: http://www.theses.fr/2015PA114834

17. Bessede, Emilie. Etude des mécanismes de la carcinogénèse gastrique induite par Helicobacter pylori impliquant la transition épithélio-mésenchymateuse : Study of gastric carcinogenesis induced by helicobacter pylori and implicating the epithelial to mesenchymal transition.

Degree: Docteur es, Sciences, technologie, santé. Microbiologie-Immunologie, 2012, Université de Bordeaux Segalen

URL: http://www.theses.fr/2012BOR21955

►

L’infection par Helicobacter pylori touche environ la moitié de la population mondiale et est responsable de plusieurs pathologies gastro-intestinales incluant l’adénocarcinome gastrique. Les mécanismes de… (more)

▼

L’infection par Helicobacter pylori touche environ la moitié de la population mondiale et est responsable de plusieurs pathologies gastro-intestinales incluant l’adénocarcinome gastrique. Les mécanismes de la carcinogénèse induite par H. pylori ne sont pas clairement élucidés. Mais, l’oncoprotéine CagA que possèdent certaines souches est très impliquée dans la carcinogénèse gastrique ; elle induit l’apparition d’un phénotype particulier, dit colibri, qui mime une transition épithélio-mésenchymateuse (EMT). De plus, CagA déstabilise les jonctions cellulaires en perturbant la E-cadhérine. Les objectifs de ces travaux ont été de déterminer si H. pylori induit une véritable EMT et si cette EMT est à l’origine de l’émergence de cellules souches cancéreuses (CSC). De plus, nous avons étudié le rôle joué par la protéine IQGAP1, protéine assurant le maintien des jonctions cellulaires, dans la carcinogénèse gastrique induite par H. pylori. Ces travaux ont montré que H. pylori induit une EMT in vitro. Cette EMT est à l’origine de l’émergence de cellules CD44high présentant les caractéristiques de CSC. L’étude du rôle de IQGAP1 au cours de la carcinogénèse gastrique liée à H. pylori a permis de déterminer son implication dans l’apparition de lésions néoplasiques dans un modèle de souris transgéniques hétérozygotes pour IQGAP1. En outre, IQGAP1 apparaît comme une protéine dont l’expression est modifiée par l’infection à H. pylori et par l’EMT induite par cette bactérie in vitro. Nos résultats permettent de mieux comprendre le mécanisme physiopathologique de l’adénocarcinome gastrique et seront potentiellement utiles au développement de nouvelles thérapeutiques anti-cancéreuses.

Helicobacter pylori infection is found in about half of the world population and is responsible for several gastrointestinal pathologies, including gastric adenocarcinoma. The mechanisms of the carcinogenesis due to H. pylori remain unclear. However, the link with gastric adenocarcinoma is partly due to the H. pylori CagA oncoprotein. CagA is responsible for a particular cell phenotype in vitro, the “hummingbird” phenotype which corresponds to an elongation of the cells, mimicking an epithelial to mesenchymal transition (EMT). EMT participates to carcinogenesis, and is involved in the generation of cancer stem cells (CSC). Moreover, CagA destabilize the cell junctions. This study aimed to determine wether H. pylori induces a true EMT, and if so, wether this EMT can generate CSCs. The role of IQGAP1, which is a scaffold protein involved in cell adhesion, was also studied in cases of gastric carcinogenesis due to H. pylori. We demonstrated that H. pylori induces an EMT in vitro. Moreover, we showed that this EMT is responsible for the emergence of CD44high cells which have the same characteristics as the CSCs. IQGAP1 has been identified as a protein implicated in neoplastic lesion development in a transgenic mouse model heterozygous for IQGAP1. Moreover, in vitro, the expression of IQGAP1 was modified by H. pylori infection and more specifically by the…

Advisors/Committee Members: Mégraud, Francis (thesis director).

Subjects/Keywords: Adénocarcinome gastrique; Cellules souches cancéreuses; CD44; IQGAP1; Gastric adenocarcinoma; Cancer stem cells; CD44; IQGAP1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bessede, E. (2012). Etude des mécanismes de la carcinogénèse gastrique induite par Helicobacter pylori impliquant la transition épithélio-mésenchymateuse : Study of gastric carcinogenesis induced by helicobacter pylori and implicating the epithelial to mesenchymal transition. (Doctoral Dissertation). Université de Bordeaux Segalen. Retrieved from http://www.theses.fr/2012BOR21955

Chicago Manual of Style (16^th Edition):

Bessede, Emilie. “Etude des mécanismes de la carcinogénèse gastrique induite par Helicobacter pylori impliquant la transition épithélio-mésenchymateuse : Study of gastric carcinogenesis induced by helicobacter pylori and implicating the epithelial to mesenchymal transition.” 2012. Doctoral Dissertation, Université de Bordeaux Segalen. Accessed January 26, 2021. http://www.theses.fr/2012BOR21955.

MLA Handbook (7^th Edition):

Bessede, Emilie. “Etude des mécanismes de la carcinogénèse gastrique induite par Helicobacter pylori impliquant la transition épithélio-mésenchymateuse : Study of gastric carcinogenesis induced by helicobacter pylori and implicating the epithelial to mesenchymal transition.” 2012. Web. 26 Jan 2021.

Vancouver:

Bessede E. Etude des mécanismes de la carcinogénèse gastrique induite par Helicobacter pylori impliquant la transition épithélio-mésenchymateuse : Study of gastric carcinogenesis induced by helicobacter pylori and implicating the epithelial to mesenchymal transition. [Internet] [Doctoral dissertation]. Université de Bordeaux Segalen; 2012. [cited 2021 Jan 26]. Available from: http://www.theses.fr/2012BOR21955.

Council of Science Editors:

Bessede E. Etude des mécanismes de la carcinogénèse gastrique induite par Helicobacter pylori impliquant la transition épithélio-mésenchymateuse : Study of gastric carcinogenesis induced by helicobacter pylori and implicating the epithelial to mesenchymal transition. [Doctoral Dissertation]. Université de Bordeaux Segalen; 2012. Available from: http://www.theses.fr/2012BOR21955

University of Vienna

18. Mateos, Borja. The hidden order of intrinsically disordered proteins and its role in molecular recognition.

Degree: 2019, University of Vienna

URL: http://othes.univie.ac.at/60692/

►

Intrinsisch ungeordnete Proteine (IDP; englisch: intrinsically disordered proteins) liegen als Ensemble von schnell ineinander überführbaren Strukturen vor. Sie nehmen keine stabile tertiäre Struktur an und… (more)

▼

Intrinsisch ungeordnete Proteine (IDP; englisch: intrinsically disordered proteins) liegen als Ensemble von schnell ineinander überführbaren Strukturen vor. Sie nehmen keine stabile tertiäre Struktur an und können einen großen Konformationsraum abdecken. IDPs unterscheiden sich jedoch insofern von denaturierten Proteinen, als dass sie bestimmte kompakte energetisch bevorzugte Zustände einnehmen können. Der Grad an Unordnung bzw. Verdichtung ist in ihrer Primärstruktur codiert, welche evolutionär auf gewisse molekulare Eigenschaften und deren spezielle Funktionen optimiert wurde. Insbesondere das Fehlen einer stabilen Struktur verleiht IDPs ein Explorationsverhalten, um an einer Vielzahl von Wechselwirkungen teilzunehmen. Aufgrund ihrer Fähigkeit zu posttranslationalen Modifikationen und/oder Spleißvarianten bieten IDPs Modularität und Anpassungsfähigkeit an zahlreiche biologische Prozesse. Deshalb inszenieren intrinsisch ungeordnete Bereiche oft die Signalketten in eukaryotischen Zellen. Tatsächlich stehen das abnormale Verhalten von IDPs unter anderem auch mit Krebserkrankungen und neurodegenerativen Erkrankungen in Zusammenhang. In dieser Arbeit lege ich ein besonderes Augenmerk auf das mit Metastasen assoziierte Protein Osteopontin (OPN). OPN dient dabei als ein typisches IDP; es liegt größtenteils ungeordnet vor mit einem zentralen verdichteten Zustand, der für die richtige Anordnung der wechselwirkenden Motive des Proteins verantwortlich ist. OPN ist ein extrazelluläres Protein, das mit verschiedenen Komponenten der extrazellulären Matrix (EZM) und mit zwei Familien an Membran-Rezeptoren wechselwirkt, Integrine und CD44. Das Ziel ist die Charakterisierung (i) der für die Verdichtung des IDPs entscheidenden Sequenzen, mit besonderem Augenmerk auf die Relevanz von Prolinen; (ii) des Evolutionsdrucks, bestimmte Eigenschaften von OPN-Homologen zu konservieren; (iii) der strukturellen Neuanordnung bei der Bindung an EZM-Partner sowie auch deren Membran-Rezeptoren; (iv) der Auswirkung von Phosphorylierung auf Strukturdynamik und Bindung; und (v) der ungeordneten Bereiche von CD44 und deren Bindung an OPN. Schnelle lokale Bewegungen der Polypeptidketten machen IDPs zu hervorragend geeigneten Systemen, die mit Kernspinresonanz-Spektroskopie (NMR; englisch: nuclear magnetic resonance) in Lösung untersucht werden können. Verschiedene Methoden wurden entwickelt um die Problemstellungen anzugehen. Diese reichen von maßgeschneiderten 13C-detektierten Experimenten und ein neues Protokoll, um die Synergie zwischen NMR und Zellbiologie zu nutzen und die Bindung von OPN an lebende Zellen zu charakterisieren.

Intrinsically disordered proteins (IDPs) exist as an ensemble of rapidly interconverting structures. They do not adopt a stable tertiary structure and explore a large conformational space. However, IDPs differ from denatured proteins in the sense that they probe some energetically favourable compact states. The degree of ‘disorder’ or compaction is encoded in their primary sequence, which has evolved to provide certain…

Subjects/Keywords: 35.25 Spektrochemische Analyse; 42.13 Molekularbiologie; IDP / NMR / Osteopontin / CD44; IDP / NMR / Osteopontin / CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mateos, B. (2019). The hidden order of intrinsically disordered proteins and its role in molecular recognition. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/60692/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mateos, Borja. “The hidden order of intrinsically disordered proteins and its role in molecular recognition.” 2019. Thesis, University of Vienna. Accessed January 26, 2021. http://othes.univie.ac.at/60692/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mateos, Borja. “The hidden order of intrinsically disordered proteins and its role in molecular recognition.” 2019. Web. 26 Jan 2021.

Vancouver:

Mateos B. The hidden order of intrinsically disordered proteins and its role in molecular recognition. [Internet] [Thesis]. University of Vienna; 2019. [cited 2021 Jan 26]. Available from: http://othes.univie.ac.at/60692/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mateos B. The hidden order of intrinsically disordered proteins and its role in molecular recognition. [Thesis]. University of Vienna; 2019. Available from: http://othes.univie.ac.at/60692/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Molina-Castro, Silvia. Study of the Hippo/YAP1 signaling pathway in gastric carcinogenesis induced by Helicobacter pylori : Etude de la voie de signalisation HIPPO/YAP dans la carcinogenèse gastrique induite par l'infection à Helicobacter pylori.

Degree: Docteur es, Microbiologie Immunologie, 2017, Bordeaux

URL: http://www.theses.fr/2017BORD0623

► Le cancer gastrique (CG) est une maladie multifactorielle, fréquemment associée à l’infection chronique par des souches CagA+ d’Helicobacter pylori. La transition épithélio-mésenchymateuse (EMT) est un… (more)

▼ Le cancer gastrique (CG) est une maladie multifactorielle, fréquemment associée à l’infection chronique par des souches CagA+ d’Helicobacter pylori. La transition épithélio-mésenchymateuse (EMT) est un processus réversible dans lequel une cellule épithéliale polarisée acquiert un phénotype mésenchymateux. L’EMT est à l’émergence de cellules souches cancéreuses (CSC) qui expriment CD44 et présentent une activité ALDH élevée. L’infection des cellules épithéliales gastriques humaines (CEGs) par CagA+ H. pylori induit des cellules CD44+ avec des propriétés des CSCs via une EMT. La voie Hippo est composée par les kinases MST et LATS, et leurs cibles, les YAP1 et TAZ. Suite à la phosphorylation, YAP1 et TAZ sont inhibés. YAP1 et TAZ activés lient les facteurs TEAD pour promouvoir la croissance cellulaire et l’inhibition de l’apoptose.Notre premier objectif était de rechercher si H. pylori change l’état d’activation de la voie Hippo et l'effet sur l’EMT et les CSC in vitro et in vivo. Le deuxième but est la caractérisation du rôle de YAP1/TEAD dans les propriétés de CSCs gastriques in vitro et les conséquences de son inhibition dans la croissance tumorale in vivo.Pour étudier la régulation de la voie Hippo pendant l’infection par H. pylori, LATS2, YAP1 et CD44 ont été évalués dans la muqueuse gastrique de sujets non-infectés et infectés par H. pylori, qui ont été augmentés avec l’infection et leur surexpression a été associée avec la gastrite et la métaplasie intestinale. Dans les CEGs l’expression de gènes de la voie Hippo a été altérée par l’infection. La régulation de la voie Hippo par H. pylori a une cinétique diphasique et dépendante de CagA. Dans l’infection précoce, H. pylori déclenche l’activité transcriptionelle de YAP1. Cette période d’inactivité de la voie Hippo est suivi de son activation progressive, soutenue par l’accumulation de LATS2 et la phosphorylation inhibitrice de YAP1. La répression de LATS2 avec siRNAs a accéléré l’acquisition du phénotype mésenchymateux après l’infection, l’augmentation de marqueurs de l’EMT (Zeb1 et Snail1), et la diminution des miR-200 épithéliaux. Les CSC induites par H. pylori ont été potentialisées par l’inhibition de LATS2, ce qui suggère que LATS2 limite l’EMT et le phénotype de CSC acquis pendant l’infection. L’inhibition de LATS2 ou YAP1 diminue l’expression de ces deux protéines, révélant ainsi une boucle de régulation positive. Dans des coupes de tissu de CG, l’expression de LATS2 et YAP1 est hétérogène et positivement corrélée, fait qui a été confirmé dans 38 CEGs de la CCLE. L’expression LATS2 est fortement corrélée à celle de CTGF et CYR61, ce qui suggère que LATS2 peut aussi être un gène cible de YAP1/TEAD.La verteporfine (VP) est capable d’interrompre l’interaction YAP1/TEAD, et donc d’inhiber son activité transcriptionelle. In vitro, utilisant CEGs et des cellules de tumeurs de patients amplifiées chez la souris (patient-derived xenograft PDX), le traitement à la VP a diminué la croissance cellulaire, l’expression de gènes cible de YAP1/TAZ/TEAD, l’activité du… Advisors/Committee Members: Varon, Christine (thesis director).

Subjects/Keywords: Cellules souches gastriques; Transition épithélio-mésenchymateuse; CD44; Cancer stem cells; Epithelial to mesenchymal transition; CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Molina-Castro, S. (2017). Study of the Hippo/YAP1 signaling pathway in gastric carcinogenesis induced by Helicobacter pylori : Etude de la voie de signalisation HIPPO/YAP dans la carcinogenèse gastrique induite par l'infection à Helicobacter pylori. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2017BORD0623

Chicago Manual of Style (16^th Edition):

Molina-Castro, Silvia. “Study of the Hippo/YAP1 signaling pathway in gastric carcinogenesis induced by Helicobacter pylori : Etude de la voie de signalisation HIPPO/YAP dans la carcinogenèse gastrique induite par l'infection à Helicobacter pylori.” 2017. Doctoral Dissertation, Bordeaux. Accessed January 26, 2021. http://www.theses.fr/2017BORD0623.

MLA Handbook (7^th Edition):

Molina-Castro, Silvia. “Study of the Hippo/YAP1 signaling pathway in gastric carcinogenesis induced by Helicobacter pylori : Etude de la voie de signalisation HIPPO/YAP dans la carcinogenèse gastrique induite par l'infection à Helicobacter pylori.” 2017. Web. 26 Jan 2021.

Vancouver:

Molina-Castro S. Study of the Hippo/YAP1 signaling pathway in gastric carcinogenesis induced by Helicobacter pylori : Etude de la voie de signalisation HIPPO/YAP dans la carcinogenèse gastrique induite par l'infection à Helicobacter pylori. [Internet] [Doctoral dissertation]. Bordeaux; 2017. [cited 2021 Jan 26]. Available from: http://www.theses.fr/2017BORD0623.

Council of Science Editors:

Molina-Castro S. Study of the Hippo/YAP1 signaling pathway in gastric carcinogenesis induced by Helicobacter pylori : Etude de la voie de signalisation HIPPO/YAP dans la carcinogenèse gastrique induite par l'infection à Helicobacter pylori. [Doctoral Dissertation]. Bordeaux; 2017. Available from: http://www.theses.fr/2017BORD0623

20. MarkÃnia KÃlia Santos Alves. Identifying of molecular alterations associated to expression of CD133, CXCR4, CD44 and OLIG2 and CDKN2A methylation in promoter in astrocytic tumors.

Degree: PhD, 2014, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14421 ;

► Tumores sÃo populaÃÃes celulares heterogÃneas hierarquicamente organizadas, cujas cÃlulas-tronco possuem importÃncia relevante desde que sÃo cÃlulas com a capacidade de se renovarem e de gerarem… (more)

▼ Tumores sÃo populaÃÃes celulares heterogÃneas hierarquicamente organizadas, cujas cÃlulas-tronco possuem importÃncia relevante desde que sÃo cÃlulas com a capacidade de se renovarem e de gerarem linhagens em fases diferentes. Dada a sua importÃncia, a identificaÃÃo de componentes de cÃlulas-tronco Ã essencial para o entendimento da tumorigÃnese. Apesar de marcadores de linhagem neural terem sido identificados, a associaÃÃo destes marcadores com os tumores cerebrais ainda Ã escassa e nos astrocitomas sÃo relacionados principalmente aos glioblastomas. Entre esses marcadores de cÃlulas-tronco,CD133, CXCR4 e CD44 sÃo relacionados Ã formaÃÃo do glioma, migraÃÃo e crescimento; por outro lado, OLIG2 Ã envolvido no destino celular. NÃo existem estudos, atÃ essa data, que avaliam todos esses marcadores juntos e sua relaÃÃo com grau tumoral. Adicionalmente, alteraÃÃes epigenÃticas especÃficas, especialmente a metilaÃÃo em promotor, tem sido identificadas nestes tumores, levando a inativaÃÃo de genes, com destaque o CDKN2A (proteÃna p16INK4A), um supressor tumoral. Apesar de esse mecanismo ser apontado como o principal inativador desse gene, em astrocitomas ainda existem questÃes controversas. Para avaliar essas questÃes, este estudo objetivou determinar a expressÃo e padrÃo de metilaÃÃo em promotor de CDKN2A e sua associaÃÃo com parÃmetros clinico-patolÃgicos e se a presenÃa de cÃlulas-tronco/progenitoras, considerando a expressÃo de CD133, CXCR4, CD44 e OLIG2 poderia definir subpopulaÃÃes de cÃlulas que podem ser usadas como marcadores prognÃsticos. Para isso, em uma sÃrie de 93 astrocitomas de diferentes graus de malignidade, foram estudadas a expressÃo dos marcadores CD133, CXCR4, CD44, OLIG2 e p16INK4A, detectada pela tÃcnica de imunohistoquÃmica, e o padrÃo de metilaÃÃo em promotor de CDKN2A, por PCR especÃfico para metilaÃÃo (PCR-MS). Os dados foram entÃo associados com grau tumoral, localizaÃÃo e outros parÃmetros clinico-patolÃgicos. As anÃlises estatÃsticas foram realizadas usando o teste do X2, teste exato de Fisher, correlaÃÃo de Spearman, agrupamento de k-means e anÃlise de componentes principais, com diferenÃas consideradas significantes com p<0.05. A imunomarcaÃÃo de OLIG2 mostrou a frequÃncia maior de positividade (73,1%), seguido por CXCR4 (60,2%), CD44 (55,9%) e CD133 (45,2%). AnÃlises de correlaÃÃo e agrupamento definiram dois subtipos de populaÃÃo de acordo com os marcadores estudados, um subtipo CXCR4(+)CD133(+)CD44(+) e outro OLIG2(+). Tumores CD133, CXCR4 e CD44 positivos aumentaram de acordo com malignidade. No grau IV, este subtipo de tumores [CD133(+)CXCR4(+)CD44(+)] foi significantemente mais frequente (p=0,008) e tambÃm nos tumores difusos. Adicionalmente, tumores com CXCR4(+) e CD133(+) foram preferencialmente localizados nos hemisfÃrios cerebrais e nos ventrÃculos, e a maioria nos pacientes com idade ≥ 30 anos. Por outro lado, tumores OLIG2(+) foram associados com o cerebelo, que Ã a localizaÃÃo preferencial do astrocitoma pilocÃtico. Uma forte correlaÃÃo negativa entre imunomarcaÃÃo nuclear… Advisors/Committee Members: Geanne Matos de Andrade, JosÃ Arnaldo Motta de Arruda, Rommel Mario RodrÃguez Burba, Alba Fabiola Costa Torres, Silvia Helena Barem Rabenhorst.

Subjects/Keywords: CANCEROLOGIA; Astrocitomas; MetilaÃÃo; p16; CÃlulas-tronco; CD133, CXCR4; CD44; OLIG2; Astrocytoma; Methylation; p16; Stem-cell; CD133, CXCR4; CD44; OLIG2; Tumores

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alves, M. K. S. (2014). Identifying of molecular alterations associated to expression of CD133, CXCR4, CD44 and OLIG2 and CDKN2A methylation in promoter in astrocytic tumors. (Doctoral Dissertation). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14421 ;

Chicago Manual of Style (16^th Edition):

Alves, MarkÃnia KÃlia Santos. “Identifying of molecular alterations associated to expression of CD133, CXCR4, CD44 and OLIG2 and CDKN2A methylation in promoter in astrocytic tumors.” 2014. Doctoral Dissertation, Universidade Federal do Ceará. Accessed January 26, 2021. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14421 ;.

MLA Handbook (7^th Edition):

Alves, MarkÃnia KÃlia Santos. “Identifying of molecular alterations associated to expression of CD133, CXCR4, CD44 and OLIG2 and CDKN2A methylation in promoter in astrocytic tumors.” 2014. Web. 26 Jan 2021.

Vancouver:

Alves MKS. Identifying of molecular alterations associated to expression of CD133, CXCR4, CD44 and OLIG2 and CDKN2A methylation in promoter in astrocytic tumors. [Internet] [Doctoral dissertation]. Universidade Federal do Ceará 2014. [cited 2021 Jan 26]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14421 ;.

Council of Science Editors:

Alves MKS. Identifying of molecular alterations associated to expression of CD133, CXCR4, CD44 and OLIG2 and CDKN2A methylation in promoter in astrocytic tumors. [Doctoral Dissertation]. Universidade Federal do Ceará 2014. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14421 ;

21. Valejo, Fernando Antonio Mourão. Transição epitélio-mesenquimal e presença de células CD44+/CD24- como fatores de predição de metástase axilar no câncer de mama inicial.

Degree: Mestrado, Ginecologia e Obstetrícia, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-18102010-151920/ ;

►

Sabemos hoje que os tumores sólidos apresentam uma composição celular heterogênia e que apenas uma pequena parcela dessas células apresenta capacidade de se proliferar e… (more)

▼

Sabemos hoje que os tumores sólidos apresentam uma composição celular heterogênia e que apenas uma pequena parcela dessas células apresenta capacidade de se proliferar e gerar novos tumores. Estudos prévios sobre a formação do câncer de mama têm sido realizados com base na combinação dos marcadores de superfície celular CD44 e CD24. Já foi demonstrado que uma subpopulação de células do câncer de mama com alta expressão de CD44 e baixa expressão de CD24 (CD44+/CD24-) tem maior capacidade de gerar tumores, quando comparadas com a subpopulação de células CD44-/CD24+. O objetivo do estudo foi identificar a taxa de células com fenótipo CD44+/CD24- presentes nos tumores mamários e relaciona-la com a taxa de comprometimento dos linfonodos axilares ipsilaterais por neoplasia, além de avaliar também sua relação com outros fatores sabidamente relacionados com mal prognóstico da paciente. Pacientes e métodos: avaliamos prospectivamente 53 amostras cirúrgicas provenientes de 42 pacientes com diagnóstico histopatológico de carcinoma de mama, quantificando as células CD44+/CD24- por citometria de fluxo. Relacionamos a porcentagem destas células encontrada em cada amostra com o comprometimento axilar, os receptores hormonais e Her-2, a idade da paciente, o grau histológico do tumor, o diâmetro patológico do tumor e o tipo histológico. Resultados: verificamos um significante aumento da população de células CD44+/CD24- no grupo de carcinomas ductais invasivos em pacientes que apresentavam metástase axilar [mediana 8,53% (3,6 71,2%)] em relação ao grupo de pacientes sem linfonodos comprometidos pela neoplasia [mediana 1,49% (0,3 17,1%)] (p=0,0002). Conclusão: concluímos então que quando estudamos vários tumores mamários invasivos de mesma classificação histológica, podemos notar que existe uma variação na quantidade de células CD44+/CD24- entre eles. Nosso estudo mostrou que essa variação está relacionada à agressividade tumoral e à sua capacidade de gerar metástases já que, tumores com maior quantidade de células CD44+/CD24- apresentam maior taxa de comprometimento dos linfonodos axilares.

It is known that solid tumors are composed by a heterogeneous combination of cells and only a small portion of these cells has the capacity to proliferate and generate new tumors. Previous studies about the breast cancer initiation have been based on a combination of CD44 and CD24cell surface markers. It has been shown that this subpopulation of breast cancer cells with high expression of CD44 and low expression of CD24 (CD44+/CD24-) has a greater capacity to generate tumors when compared with the subpopulation of cells CD44- /CD24+. The study objective was to identify whether the rate of cells with CD44+/CD24- phenotype present in breast tumors is related with the rate of ipsilateral lymph node metastasis, in addition to evaluate its relationship with other risk factors known to be related with worst prognosis. Patients and methods: we prospectively evaluated 53 surgical specimens from 42 patients with histological diagnosis of breast cancer,…

Advisors/Committee Members: Tiézzi, Daniel Guimarães.

Subjects/Keywords: Axillary metastasis; Breast cancer; Câncer de mama; CD44+/CD24- cells; Células CD44+/CD24-; Epithelial-mesenchymal transition; Metástase axilar; Transição epitélio-mesenquimal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Valejo, F. A. M. (2010). Transição epitélio-mesenquimal e presença de células CD44+/CD24- como fatores de predição de metástase axilar no câncer de mama inicial. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17145/tde-18102010-151920/ ;

Chicago Manual of Style (16^th Edition):

Valejo, Fernando Antonio Mourão. “Transição epitélio-mesenquimal e presença de células CD44+/CD24- como fatores de predição de metástase axilar no câncer de mama inicial.” 2010. Masters Thesis, University of São Paulo. Accessed January 26, 2021. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-18102010-151920/ ;.

MLA Handbook (7^th Edition):

Valejo, Fernando Antonio Mourão. “Transição epitélio-mesenquimal e presença de células CD44+/CD24- como fatores de predição de metástase axilar no câncer de mama inicial.” 2010. Web. 26 Jan 2021.

Vancouver:

Valejo FAM. Transição epitélio-mesenquimal e presença de células CD44+/CD24- como fatores de predição de metástase axilar no câncer de mama inicial. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2021 Jan 26]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-18102010-151920/ ;.

Council of Science Editors:

Valejo FAM. Transição epitélio-mesenquimal e presença de células CD44+/CD24- como fatores de predição de metástase axilar no câncer de mama inicial. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-18102010-151920/ ;

22. Bouga, Eleni. Η συμμετοχή του υποδοχέα CD44 και των βιοσυνθετικών και καταβολικών ενζύμων του υαλουρονικού οξέος στον καρκίνο του παχέος εντέρου.

Degree: 2013, University of Patras; Πανεπιστήμιο Πατρών

URL: http://hdl.handle.net/10442/hedi/37904

► Colon cancer comes third worldwide in men and fourth in women. During progression to colon cancer, the first disorder observed is the increase of the… (more)

▼ Colon cancer comes third worldwide in men and fourth in women. During progression to colon cancer, the first disorder observed is the increase of the cells number (hyperplasia) at the intestinal epithelial surface. As a result, adenomas formed increase in size and number, without invading in neighboring structures. Some of the adenomas can become in situ cancer and finally invasive cancer. Extracellular matrix surrounds the cells and supports their growth into the tissues. Particularly, the molecules of the extracellular matrix interact with the cells, signal them and affect important cellular functions, such as recognition between cells, cell adhesion, migration, proliferation and differentiation. In cancer, structural changes take place in the synthesis and degradation of the extracellular matrix, leading to increased deposition of molecules in the matrix. As normal cells become cancerous, the extracellular matrix is modified in such a way that promotes the secretion of molecules, which further promote the growth of cancer cells and promote processes such as filtration and metastasis. The extracellular matrix, therefore, is one that provides in tumor cells survival signals, such as proliferation, motility, infiltration and metastasis. Hyaluronan is a main component of the extracellular matrix, which participates in a variety of molecular functions, which contribute both to structural features of the tissue and on the cell behavior during the formation or reorganization of tissues. Most cancers are characterized by an increased accumulation of hyaluronan. In most normal biological processes, large molecules of hyaluronan are usually found, whereas in cancerous conditions small molecules of hyaluronan are mostly found, which are considered to facilitate the mobility of cancer cells and infiltration. In the production of molecules of hyaluronan, its biosynthetic enzymes play catalytic role, hyaluronan synthases (HAS1, HAS2, HAS3), and its catabolic enzymes, hyaluronidases (Hyal-1, -2, -3, PH-20). The three synthases, while they have high homology in their sequence, they differ in the sizes of synthesized molecules. Hyaluronidases have also high degree of homology, they are transcriptionally active and their expression depends on the type of tissue in which they are expressed. During tumor development differential expression of synthases is observed and the transcriptional regulation of genes allows the cells to modulate the extracellular environment so as to favor tumor growth and metastasis. Additionally, numerous studies show differential hyaluronidase expression in cancer, which suggests that cancer cells use hyaluronidase to cleave hyaluronan in tissues and thereby facilitate tumor invasion. Furthermore, loss of hyaluronolytic activity leads to accumulation of hyaluronan that may be one of the steps required by the cells during carcinogenesis. All these molecules are involved in hyaluronan metabolism and in the reorganization of the extracellular matrix, in cell-extracellular matrix interactions and are likely…

Subjects/Keywords: Υαλουρονικό οξύ; Καρκίνος παχέος εντέρου; Συνθάσες του υαλουρονικού; Υαλουρονιδάσες; Υποδοχέας CD44; Hyaluronan; Colon cancer; Hyaluronan synthases; Hyaluronidases; Receptor CD44

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bouga, E. (2013). Η συμμετοχή του υποδοχέα CD44 και των βιοσυνθετικών και καταβολικών ενζύμων του υαλουρονικού οξέος στον καρκίνο του παχέος εντέρου. (Thesis). University of Patras; Πανεπιστήμιο Πατρών. Retrieved from http://hdl.handle.net/10442/hedi/37904

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bouga, Eleni. “Η συμμετοχή του υποδοχέα CD44 και των βιοσυνθετικών και καταβολικών ενζύμων του υαλουρονικού οξέος στον καρκίνο του παχέος εντέρου.” 2013. Thesis, University of Patras; Πανεπιστήμιο Πατρών. Accessed January 26, 2021. http://hdl.handle.net/10442/hedi/37904.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bouga, Eleni. “Η συμμετοχή του υποδοχέα CD44 και των βιοσυνθετικών και καταβολικών ενζύμων του υαλουρονικού οξέος στον καρκίνο του παχέος εντέρου.” 2013. Web. 26 Jan 2021.

Vancouver:

Bouga E. Η συμμετοχή του υποδοχέα CD44 και των βιοσυνθετικών και καταβολικών ενζύμων του υαλουρονικού οξέος στον καρκίνο του παχέος εντέρου. [Internet] [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2013. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10442/hedi/37904.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bouga E. Η συμμετοχή του υποδοχέα CD44 και των βιοσυνθετικών και καταβολικών ενζύμων του υαλουρονικού οξέος στον καρκίνο του παχέος εντέρου. [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2013. Available from: http://hdl.handle.net/10442/hedi/37904

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Aldehaiman, Mansour M. Unveiling the role of PAK2 in CD44 mediated inhibition of proliferation, differentiation and apoptosis in AML cells.

Degree: 2018, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/627947

► Acute myeloid leukemia (AML) is a heterogeneous disease characterized by the accumulation of immature nonfunctional highly proliferative hematopoietic cells in the blood, due to a… (more)

▼ Acute myeloid leukemia (AML) is a heterogeneous disease characterized by the accumulation of immature nonfunctional highly proliferative hematopoietic cells in the blood, due to a blockage in myeloid differentiation at various stages. Since the success of the differentiation agent, All-trans retinoic acid (ATRA), in the treatment of acute promyelocytic leukemia (APL), much effort has gone into trying to find agents that are able to differentiate AML cells and specifically the leukemic stem cell (LSC). CD44 is a cell surface receptor that is over-expressed on AML cells. When bound to anti-CD44 monoclonal antibodies (mAbs), this differentiation block is relieved in AML cells and their proliferation is reduced. The molecular mechanisms that AML cells undergo to achieve this reversal of their apparent phenotype is not fully understood. To this end, we designed a study using quantitative phosphoproteomics approaches aimed at identifying differences in phosphorylation found on proteins involved in signaling pathways post-treatment with CD44-mAbs. The Rho family of GTPases emerged as one of the most transformed pathways following the treatment with CD44-mAbs. The P21 activated kinase 2(PAK2), a target of the Rho family of GTPases, was found to be differentially phosphorylated in AML cells post-treatment with CD44-mAbs. This protein has been found to possess a role similar to that of a switch that determines whether the cell survives or undergoes apoptosis. Beyond confirming these results by various biochemical approaches, our study aimed to determine the effect of knock down of PAK2 on AML cell proliferation and differentiation. In addition, over-expression of PAK2 mutants using plasmid cloning was also explored to fully understand how levels of PAK2 as well as the alteration of specific phospohorylation sites could alter AML cell responses to CD44-mAbs. Results from this study will be important in determining whether PAK2 could be used as a potential therapeutic target for AML once its levels are altered.

Subjects/Keywords: Leukemia; AML; Anti-CD44; PAK2; CD44; HL60

…18 Figure 4. CD44 protein… …21 Figure 5. IPA analysis of phosphoproteomic study performed on CD44-mAb treated HL60… …25 Figure 8. Anti-CD44 mAb (A3D8) treatment inhibits the proliferation of HL60… …cells. ........... 40 Figure 9. Anti-CD44 mAb (A3D8) treatment inhibits the… …proliferation of KG1a cells............. 41 Figure 10. Anti-CD44 mAb (A3D8) treatment…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aldehaiman, M. M. (2018). Unveiling the role of PAK2 in CD44 mediated inhibition of proliferation, differentiation and apoptosis in AML cells. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/627947

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aldehaiman, Mansour M. “Unveiling the role of PAK2 in CD44 mediated inhibition of proliferation, differentiation and apoptosis in AML cells.” 2018. Thesis, King Abdullah University of Science and Technology. Accessed January 26, 2021. http://hdl.handle.net/10754/627947.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aldehaiman, Mansour M. “Unveiling the role of PAK2 in CD44 mediated inhibition of proliferation, differentiation and apoptosis in AML cells.” 2018. Web. 26 Jan 2021.

Vancouver:

Aldehaiman MM. Unveiling the role of PAK2 in CD44 mediated inhibition of proliferation, differentiation and apoptosis in AML cells. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2018. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10754/627947.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aldehaiman MM. Unveiling the role of PAK2 in CD44 mediated inhibition of proliferation, differentiation and apoptosis in AML cells. [Thesis]. King Abdullah University of Science and Technology; 2018. Available from: http://hdl.handle.net/10754/627947

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

24. Li, Wen-yuan. Immunohistochemical study for DLK-1 expression in ovarian cancers.

Degree: Master, Biological Sciences, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806116-110828

► Ovarian carcinoma has poor prognosis with a high mortality rate in gynecologic malignancy. The epithelial ovarian cancers account for 90% of ovarian malignancy. They have… (more)

▼ Ovarian carcinoma has poor prognosis with a high mortality rate in gynecologic malignancy. The epithelial ovarian cancers account for 90% of ovarian malignancy. They have been considered arising from the surface epithelium covering the ovary. The major types of epithelial ovarian cancers include serous, endometrioid, mucinous and clear cell carcinomas. DLK-1(Delta-Like 1 Homolog) is a transmembranous and secreted protein belonging to the epidermal growth factor-like homeotic family. Although expressed widely during embryonic development, only few types of tissue retain DLK-1 expression in adults. DLK-1 plays important roles in regulating cell differentiation, such as adipogenesis and osteogenesis. Aberrant expression of DLK1 has been found in various types of human cancers, including neuroblastoma, hepatocellular carcinoma, glioma and prostate cancer, but its expression in ovarian cancer has not yet been studied.CD44 is known as a stem cell marker. Previous studies showed that CD44 expressed higher level in ovarian cancer tissues and has been identified as a biomarker of ovarian carcinoma. Vimentin is a type III intermediate filament protein that is widely expressed in mesenchymal tissue, and is considered an important marker for epithelial-mesenchymal transition (EMT). E-cadherin is widely expressed in epithelial cells and acts as a pivotal tumor suppressor. Loss of E-cadherin expression has been known to be involved in epithelial-mesenchymal transition. This study was aimed to discover the significance of DLK-1 expression and its correlation with tumor stemness and EMT in various types of epithelial ovarian cancer. The expression of DLK-1, CD44, vimentin and E-cadherin were analyzed in 283 ovarian carcinomas by immunohistochemistry on tissue microarray. The result showed positive correlation among DLK-1, CD44 and Vimentin expressions (P ï¼ 0.01). Although there was no statistical significance (P ï¼ 0.237), a reversed correlation was noted between the expressions of DLK-1 and E-cadherin in ovarian carcinomas. Our study revealed that DLK-1 is overexpressed in epithelial ovarian cancers, especially in endometrioid carcinoma. In summary, DLK-1 may correlate with EMT and tumor stemness of ovarian carcinoma. Further study is needed to assess the potential use of DLK-1 for therapeutic strategy and prognosis evaluation. Advisors/Committee Members: Ming-Hong Tai (chair), Chen-Hsuan Wu (chair), Chao-Cheng Huang (committee member), Jiin-Tsuey Cheng (committee member).

Subjects/Keywords: E-cadherin; Vimentin; CD44; DLK-1; Ovarian carcinoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, W. (2016). Immunohistochemical study for DLK-1 expression in ovarian cancers. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806116-110828

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Wen-yuan. “Immunohistochemical study for DLK-1 expression in ovarian cancers.” 2016. Thesis, NSYSU. Accessed January 26, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806116-110828.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Wen-yuan. “Immunohistochemical study for DLK-1 expression in ovarian cancers.” 2016. Web. 26 Jan 2021.

Vancouver:

Li W. Immunohistochemical study for DLK-1 expression in ovarian cancers. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 26]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806116-110828.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li W. Immunohistochemical study for DLK-1 expression in ovarian cancers. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0806116-110828

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Norte

25. Moura, Jamile Marinho Bezerra de Oliveira. Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores .

Degree: 2016, Universidade do Rio Grande do Norte

URL: http://repositorio.ufrn.br/handle/123456789/21273

► Salivary gland neoplasms exhibit a wide variety of biological behavior and a high morphological diversity raises the interest in researching these lesions. The stem cells… (more)

▼ Salivary gland neoplasms exhibit a wide variety of biological behavior and a high morphological diversity raises the interest in researching these lesions. The stem cells are the main source for the generation and maintenance of cell diversity, disorders in the regulation of these cells can lead to the production of altered stem cells, termed cancer stem cells capable of generate the tumor. Researches on cancer stem cells and associated proteins have been developed in some oral cancers; however, their role in salivary gland neoplasms is not well established. Thus, the aim of this study was to identify the tumor parenchyma cells exhibiting stem cell characteristics, by evaluating the immunoreactivity of OCT4 and CD44, in a number of cases of salivary gland neoplasms. The sample consisted of 20 pleomorphic adenomas, 20 mucoepidermoid carcinomas and 20 adenoid cystic carcinoma located in minor and major salivary glands. The expression of OCT4 and CD44 was evaluated by the percentage of positive cells (PP) and the intensity of expression (IE), it is realized the sum of the scores, resulting in the total score immunostaining (PIT) ranging 0-7. All studied cases showed positive expression of OCT4 and CD44 and higher values than the control groups. It was observed that for OCT4 luminal cells and non-luminal were immunostained in the case of pleomorphic adenomas and adenoid cystic carcinoma. Already the immunoreactivity of CD44 was particularly evident in the non-luminal cells of these lesions. In mucoepidermoid carcinomas for both markers, there was immunoreactivity in squamous and intermediate cells and absence of staining mucous cells. For both markers, a statistically significant higher immunostaining was verified in neoplasms located in the major salivary glands compared with lesions in the minor salivary (p<0.001). At the total sample and in the group of minor salivary glands, malignant neoplasms exhibited higher immunoreactivity for OCT4 than pleomorphic adenoma. However, there was no statistically significant difference between the lesions and between their classifications histomorphologic. Analyzing the correlation between OCT4 and CD44 immunoexpressions, a statistically significant moderate positive correlation (r = 0.444) was observed. The high expression of OCT4 and CD44 may indicate that these proteins play an important role in identifying cancer stem cells, allowing a prediction of biological behavior of salivary gland neoplasms. Advisors/Committee Members: Souza, Lelia Batista de (advisor), 08588724472 (advisor), http://lattes.cnpq.br/6671914892609743 (advisor).

Subjects/Keywords: Células tronco-tumorais; OCT4; CD44; Imuno-histoquímica; Glândulas salivares

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moura, J. M. B. d. O. (2016). Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores . (Doctoral Dissertation). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/21273

Chicago Manual of Style (16^th Edition):

Moura, Jamile Marinho Bezerra de Oliveira. “Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores .” 2016. Doctoral Dissertation, Universidade do Rio Grande do Norte. Accessed January 26, 2021. http://repositorio.ufrn.br/handle/123456789/21273.

MLA Handbook (7^th Edition):

Moura, Jamile Marinho Bezerra de Oliveira. “Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores .” 2016. Web. 26 Jan 2021.

Vancouver:

Moura JMBdO. Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores . [Internet] [Doctoral dissertation]. Universidade do Rio Grande do Norte; 2016. [cited 2021 Jan 26]. Available from: http://repositorio.ufrn.br/handle/123456789/21273.

Council of Science Editors:

Moura JMBdO. Análise da imunoexpressão de OCT4 e CD44 em neoplasias de glândulas salivares menores e maiores . [Doctoral Dissertation]. Universidade do Rio Grande do Norte; 2016. Available from: http://repositorio.ufrn.br/handle/123456789/21273

26. Θεοδωροπούλου, Ανδριάνα. Μελέτη της έκφρασης σηματοδοτικών μορίων που εμπλέκονται στη βιολογική συμπεριφορά των μηνιγγιωμάτων του ανθρώπου.

Degree: 2012, University of Patras

URL: http://hdl.handle.net/10889/5448

►

Τα μηνιγγιώματα αποτελούν ιδιαίτερα συχνούς όγκους του Kεντρικού Νευρικού Συστήματος, όχι όμως επαρκώς μελετημένοι. Στην πλειοψηφία τους είναι καλοήθεις όγκοι (WHO grade I), όμως το… (more)

▼

Τα μηνιγγιώματα αποτελούν ιδιαίτερα συχνούς όγκους του Kεντρικού Νευρικού Συστήματος, όχι όμως επαρκώς μελετημένοι. Στην πλειοψηφία τους είναι καλοήθεις όγκοι (WHO grade I), όμως το 10% όλων των μηνιγγιωμάτων εμφανίζουν κακοήθη χαρακτηριστικά, όπως διήθηση των παρακείμενων ιστών, αυξημένα ποσοστά υποτροπής μετά από εξαίρεση, κ.α. Το σηματοδοτικό μονοπάτι Hippo είναι ένα διατηρημένο εξελικτικά μονοπάτι που εμπλέκεται σε διαδικασίες ρύθμισης του μεγέθους των οργάνων, κυτταρικού πολλαπλασιασμού, διαφοροποίησης και ανάπτυξης καρκίνου. Λίγα είναι γνωστά όσον αφορά το ρόλο του Hippo μονοπατιού στα μηνιγγιώματα. Σκοπός της παρούσας εργασίας είναι η μελέτη του Hippo μονοπατιού στα χαμηλής και υψηλής κακοήθειας μηνιγγιώματα. Χρησιμοποιήθηκαν 53 δείγματα από εξαιρεθέντα μηνιγγιώματα, 34 χαμηλής κακοήθειας και 19 υψηλής κακοήθειας, τα οποία μελετήθηκαν ανοσοϊστοχημικά ως προς την έκφραση των παραγόντων του Hippo CD44, YAP και TAZ. Παρατηρήσαμε στατιστικά σημαντική συσχέτιση μεταξύ της έκφρασης των CD44, ΥΑΡ και ΤΑΖ. Υπήρχε στατιστικά σημαντική συσχέτιση μεταξύ του βαθμού κακοήθειας και της έκφρασης των παραπάνω μορίων. Παρατηρήθηκε στατιστικά σημαντική διαφορά στην πυρηνική εντόπιση των ΥΑΡ και ΤΑΖ μεταξύ των υψηλής και χαμηλής κακοήθειας μηνιγγιωμάτων. Βάσει των παραπάνω αποτελεσμάτων, συμπεραίνουμε ότι το σηματοδοτικό μονοπάτι Hippo είναι απενεργοποιημένο στα υψηλής κακοήθειας μηνιγγιώματα με αποτέλεσμα οι μεταγραφικοί συνενεργοποιητές ΥΑΡ και ΤΑΖ να δρουν στον πυρήνα προάγοντας τον κυτταρικό πολλαπλασιασμό και διαδικασίες αντιαπόπτωσης. Αυτό, είναι ίσως εφικτό μέσω της δράσης του CD44 που αλληλεπιδρά με την μερλίνη, upstream ρυθμιστής του μονοπατιού. Απαιτούνται περαιτέρω μελέτες για τη διευκρίνηση του ακριβούς μηχανισμού.

Meningiomas are among the most common primary tumors of the Central Nervous System, but relatively understudied. The majority of meningiomas are benign tumors (WHO grade I), but almost 10% of all diagnosed meningiomas exhibit malignant features, such as invasiveness to the surrounding brain tissue, high recurrence rate, etc. Hippo signaling pathway is an evolutionary highly conserved protein kinase cascade involved in organ size control, cell proliferation and apoptosis, differentiation and cancer development. Very few are known about the role of Hippo pathway and meningiomas. In this study we attempt to find out whether Hippo path functions differently between high and low grade meningiomas. For this purpose paraffin embedded tissues obtained from 53 patients who underwent surgical removal of meningiomas were examined histoimmunologicaly. 34 out of 53 cases were low grade meningiomas and 19 out of 53 were high grade meningiomas. The paraffin sections were immunostained with CD44, YAP and TAZ antibodies, components of the Hippo signaling pathway. We observed statistically significant association between the expression of CD44, YAP and TAZ. There was a significant correlation between high grade meningiomas and expression of the examined factors. Moreover there was a significant difference in…

Advisors/Committee Members: Αγγελάτου, Φεβρωνία, Theodoropoulou, Andriana, Πέτρου, Ελένη, Μητσάκου, Αδαμαντία.

Subjects/Keywords: Μηνιγγιώματα; 616.994 81; Meningiomas; Hippo pathway; CD44; YAP; TAZ

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Θεοδωροπούλου, �. (2012). Μελέτη της έκφρασης σηματοδοτικών μορίων που εμπλέκονται στη βιολογική συμπεριφορά των μηνιγγιωμάτων του ανθρώπου. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/5448

Chicago Manual of Style (16^th Edition):

Θεοδωροπούλου, Ανδριάνα. “Μελέτη της έκφρασης σηματοδοτικών μορίων που εμπλέκονται στη βιολογική συμπεριφορά των μηνιγγιωμάτων του ανθρώπου.” 2012. Masters Thesis, University of Patras. Accessed January 26, 2021. http://hdl.handle.net/10889/5448.

MLA Handbook (7^th Edition):

Θεοδωροπούλου, Ανδριάνα. “Μελέτη της έκφρασης σηματοδοτικών μορίων που εμπλέκονται στη βιολογική συμπεριφορά των μηνιγγιωμάτων του ανθρώπου.” 2012. Web. 26 Jan 2021.

Vancouver:

Θεοδωροπούλου �. Μελέτη της έκφρασης σηματοδοτικών μορίων που εμπλέκονται στη βιολογική συμπεριφορά των μηνιγγιωμάτων του ανθρώπου. [Internet] [Masters thesis]. University of Patras; 2012. [cited 2021 Jan 26]. Available from: http://hdl.handle.net/10889/5448.

Council of Science Editors:

Θεοδωροπούλου �. Μελέτη της έκφρασης σηματοδοτικών μορίων που εμπλέκονται στη βιολογική συμπεριφορά των μηνιγγιωμάτων του ανθρώπου. [Masters Thesis]. University of Patras; 2012. Available from: http://hdl.handle.net/10889/5448

UNIVERSIDADE FEDERAL DE OURO PRETO

27. Alessandra de Paula Carli. CORRELAÇÃO DA ATIVIDADE DO PROTEASSOMA, EXPRESSÃO DE CD44 E ENZIMAS PROTEOLÍTICAS EM EXTRATOS INTESTINAIS DE CAMUNDONGOS TRATADOS COM 1,2 DIMETILHIDRAZINA E INIBIDORES BOWMAN-BIRK.

Degree: 2011, UNIVERSIDADE FEDERAL DE OURO PRETO

URL: http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=651

►

The Bowman-Birk inhibitors (BBI) are protein molecules containing two distinct inhibitory domains for trypsin and chymotrypsin-like enzymes. The interest for this class of inhibitors is… (more)

▼

The Bowman-Birk inhibitors (BBI) are protein molecules containing two distinct inhibitory domains for trypsin and chymotrypsin-like enzymes. The interest for this class of inhibitors is based on studies which demonstrated a protective effect promoted by BBI, particularly for chemically-induced cancers. In the present work, we evaluated the effects of BBI isolated from Macrotyloma axillare and Glycine max in the prevention of colon cancers induced by intraperitoneal injections of dimethylhydrazine (DMH) during 12 weeks. Control and DMH-treated animals were fed a diet containing BBI at a dose of 0.1% (p/p) associated (DMHV) or not with the vitamins A, C and E, during 24 weeks. DMH-treated animals exhibited hystopathological alterations compatible with the formation of neoplastic polyps. In agreement with the hystological examinations, analysis of the tumor marker CD44, by Western blotting, revealed a significant increase in its expression for the DMH-treated group. Protein extracts from these tissues also contained increased proteasome-dependent proteolytic activity as judged by peptidase assays for the trypsin and chymotrypsin-like activities. In contrast, for DMH + BBI-treated animals proteasomal activity was comparable to that observed for the control group. In fact, animals given BBI alone, as a diet supplement, exhibited decreased proteasomal activity when compared to that from animals fed a normal diet. In addition, by using a sepharose-BBI affinity column, it was observed retention of enzymes displaying trypsin and chymotrypsin-like activities obtained from the lysosomal and soluble fractions. These proteolytic activities were also shown to be increased for DMH-treated animals. These results suggest that the inhibition of distinct proteolytic activities could account for the protective mechanisms produced by BBI. Collectively our analyses allowed us to conclude that BBI treatment was able to prevent intestine polyp formation, decrease any associated inflammatory processes and keep at basal levels the classic tumor marker CD44.

Os inibidores do tipo Bowman-Birk (BBI) são moléculas protéicas contendo dois domínios inibitórios distintos para enzimas semelhantes à tripsina e quimotripsina. O interesse por essa classe de inibidores decorre de estudos que demonstraram o efeito protetor dos BBI no câncer induzido quimicamente. No presente trabalho avaliou-se o efeito dos BBI isolados de Macrotyloma axillare e Glycine max na prevenção de neoplasias colo-retal induzidas por injeções intraperitoneais de dimetilhidrazina (DMH) durante 12 semanas. Os grupos de camundongos controle e testes (tratados com DMH) receberam uma dieta contendo BBI, na dose de 0,1% (p/p) associada (DMHV) ou não às vitaminas A, C e E, durante 24 semanas. Camundongos tratados com DMH apresentaram alterações histopatológicas compatíveis com a formação de pólipos neoplásicos. Em concordância com a histologia, a análise do marcador tumoral CD44, por Western blotting, revelou aumento significativo de expressão nos animais tratados com DMH e DMHV. Os…

Advisors/Committee Members: Milton Hércules Guerra de Andrade, André Talvani, Maria do Carmo Peluzio, William de Catro Borges, Rodolfo Cordeiro Giunchetti, Roseli Aparecida da Silva Gomes.

Subjects/Keywords: Expressão de CD44; Inibidores Bowman-Birk; BIOQUIMICA; Proteassoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carli, A. d. P. (2011). CORRELAÇÃO DA ATIVIDADE DO PROTEASSOMA, EXPRESSÃO DE CD44 E ENZIMAS PROTEOLÍTICAS EM EXTRATOS INTESTINAIS DE CAMUNDONGOS TRATADOS COM 1,2 DIMETILHIDRAZINA E INIBIDORES BOWMAN-BIRK. (Thesis). UNIVERSIDADE FEDERAL DE OURO PRETO. Retrieved from http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=651

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carli, Alessandra de Paula. “CORRELAÇÃO DA ATIVIDADE DO PROTEASSOMA, EXPRESSÃO DE CD44 E ENZIMAS PROTEOLÍTICAS EM EXTRATOS INTESTINAIS DE CAMUNDONGOS TRATADOS COM 1,2 DIMETILHIDRAZINA E INIBIDORES BOWMAN-BIRK.” 2011. Thesis, UNIVERSIDADE FEDERAL DE OURO PRETO. Accessed January 26, 2021. http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=651.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carli, Alessandra de Paula. “CORRELAÇÃO DA ATIVIDADE DO PROTEASSOMA, EXPRESSÃO DE CD44 E ENZIMAS PROTEOLÍTICAS EM EXTRATOS INTESTINAIS DE CAMUNDONGOS TRATADOS COM 1,2 DIMETILHIDRAZINA E INIBIDORES BOWMAN-BIRK.” 2011. Web. 26 Jan 2021.

Vancouver:

Carli AdP. CORRELAÇÃO DA ATIVIDADE DO PROTEASSOMA, EXPRESSÃO DE CD44 E ENZIMAS PROTEOLÍTICAS EM EXTRATOS INTESTINAIS DE CAMUNDONGOS TRATADOS COM 1,2 DIMETILHIDRAZINA E INIBIDORES BOWMAN-BIRK. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE OURO PRETO; 2011. [cited 2021 Jan 26]. Available from: http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=651.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carli AdP. CORRELAÇÃO DA ATIVIDADE DO PROTEASSOMA, EXPRESSÃO DE CD44 E ENZIMAS PROTEOLÍTICAS EM EXTRATOS INTESTINAIS DE CAMUNDONGOS TRATADOS COM 1,2 DIMETILHIDRAZINA E INIBIDORES BOWMAN-BIRK. [Thesis]. UNIVERSIDADE FEDERAL DE OURO PRETO; 2011. Available from: http://www.tede.ufop.br/tde_busca/arquivo.php?codArquivo=651

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

28. Haakenson, Jeremy Kenneth. The Role of Ceramide in Metastatic Processes in Breast Cancer.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/24221

► Ceramide is a bioactive sphingolipid that is capable of inducing apoptosis in mammalian cells. However, the role of ceramide in cancer metastasis remains largely unexplored.… (more)

Subjects/Keywords: ceramide; anoikis; metastasis; breast cancer; CD44; extravasation; carcinoma; epithelial-mesenchymal transition

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haakenson, J. K. (2015). The Role of Ceramide in Metastatic Processes in Breast Cancer. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/24221

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Haakenson, Jeremy Kenneth. “The Role of Ceramide in Metastatic Processes in Breast Cancer.” 2015. Thesis, Penn State University. Accessed January 26, 2021. https://submit-etda.libraries.psu.edu/catalog/24221.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Haakenson, Jeremy Kenneth. “The Role of Ceramide in Metastatic Processes in Breast Cancer.” 2015. Web. 26 Jan 2021.

Vancouver:

Haakenson JK. The Role of Ceramide in Metastatic Processes in Breast Cancer. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 26]. Available from: https://submit-etda.libraries.psu.edu/catalog/24221.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Haakenson JK. The Role of Ceramide in Metastatic Processes in Breast Cancer. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/24221

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Rodrigues, Raquel Sofia Ladeira, 1985-. Estudo da maturação eritróide com base no CD35 e no CD44 em síndromes mielodisplásicas.

Degree: 2013, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14528

► As Síndromes Mielodisplásicas (SMD) representam um grupo de doenças clonais da célula pluripotencial hematopoiética caracterizadas por hematopoiese ineficaz e aumento do risco de progressão para… (more)

Subjects/Keywords: Síndromes mielodisplásicas; Citometria de fluxo; Diferenciação eritróide; CD35; CD44; Ciências Médicas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodrigues, Raquel Sofia Ladeira, 1. (2013). Estudo da maturação eritróide com base no CD35 e no CD44 em síndromes mielodisplásicas. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodrigues, Raquel Sofia Ladeira, 1985-. “Estudo da maturação eritróide com base no CD35 e no CD44 em síndromes mielodisplásicas.” 2013. Thesis, RCAAP. Accessed January 26, 2021. https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodrigues, Raquel Sofia Ladeira, 1985-. “Estudo da maturação eritróide com base no CD35 e no CD44 em síndromes mielodisplásicas.” 2013. Web. 26 Jan 2021.

Vancouver:

Rodrigues, Raquel Sofia Ladeira 1. Estudo da maturação eritróide com base no CD35 e no CD44 em síndromes mielodisplásicas. [Internet] [Thesis]. RCAAP; 2013. [cited 2021 Jan 26]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodrigues, Raquel Sofia Ladeira 1. Estudo da maturação eritróide com base no CD35 e no CD44 em síndromes mielodisplásicas. [Thesis]. RCAAP; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

30. Ouasti, Sihem. Hyaluronic acid biomaterials for perspective peripheral nerve regeneration.

Degree: 2012, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:154812

► This project focused on the design of a cellular scaffold applicable for the promotion of peripheral nerve regeneration. Firstly, we established a correlation between the… (more)

▼ This project focused on the design of a cellular scaffold applicable for the promotion of peripheral nerve regeneration. Firstly, we established a correlation between the organization of HA/PEG co-polymeric networks to their mechanical and degradability properties; cell adhesion was conferred to all gels by the incorporation of RGD peptides. Three families of hydrogels were produced using different procedures to permit an increasing physical incorporation of HA into a PEGDA-based network. From a comparative study of rheological properties and enzymatic degradability, co-networks obtained using thiolated HA as chain transfer agent during PEGDA photo-polymerization were selected for further biological investigations, aiming to link the cellular response of L929 murine fibroblasts (phenotype, proliferation rate, metabolic activity) to the composition and the consistency of selected hydrogels. Our findings showed that there is a clear relation between increasing hardness and increasing cell spreading/proliferation rate. This study illustrated the possibility to fine tune cell/material interactions with appropriate reactive processing techniques.As a spin-off of this study, we become interested in the interplay of cellular interactions in the use of materials that contain both HA and RGD peptides, which can bind at the same time to HA receptors such as CD44 and av integrins. We focused on soluble HA derivatives, with or without dandling RGD peptides. The kinetics and the mechanistic details of both HA and HA-RGD internalization were studied in a phagocytic model (J774.2 murine macrophages). HA-RGD showed a form of synergic binding to integrins and CD44 (HA receptor), whereas its uptake remained solely regulated by CD44 dwell-time on the cell membrane. This study demonstrated that the knowledge of the rate-determining steps of the uptake of a carrier is necessary for assessing its efficiency. In this case, the presence of multiple ligands on a carrier was beneficial in some respect, but may not be optimal to overcome internalization limitations that arise from the slow turnover of the determining receptor.Finally, we studied the relation between the regulation of the expression of CD44 / RHAMM (HA receptor mediated motility) and the motility of Schwann cells (peripheral glial cells) and stem cells differentiated into a glial phenotype. Rt-PCR and immuno-assay experiments suggested that RHAMM up-regulation is associated with glial differentiation and we speculate that in the future this HA receptor could be considered as a differentiation marker. We also illustrated the importance of HA / RHAMM interaction for the motility of glial cells. These results indicate the importance of HA in mediating glial cell function during peripheral nerve regeneration and have implications for therapeutic repair strategies. Advisors/Committee Members: TERENGHI, GIORGIO G, Terenghi, Giorgio, Tirelli, Nicola.

Subjects/Keywords: Hyaluronic acid; Biomaterials; Peripheral nerve regeneration; CD44; RHAMM; Stem cells

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ouasti, S. (2012). Hyaluronic acid biomaterials for perspective peripheral nerve regeneration. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:154812

Chicago Manual of Style (16^th Edition):

Ouasti, Sihem. “Hyaluronic acid biomaterials for perspective peripheral nerve regeneration.” 2012. Doctoral Dissertation, University of Manchester. Accessed January 26, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:154812.

MLA Handbook (7^th Edition):

Ouasti, Sihem. “Hyaluronic acid biomaterials for perspective peripheral nerve regeneration.” 2012. Web. 26 Jan 2021.

Vancouver:

Ouasti S. Hyaluronic acid biomaterials for perspective peripheral nerve regeneration. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2021 Jan 26]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:154812.

Council of Science Editors:

Ouasti S. Hyaluronic acid biomaterials for perspective peripheral nerve regeneration. [Doctoral Dissertation]. University of Manchester; 2012. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:154812

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