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You searched for subject:(CARD15). Showing records 1 – 3 of 3 total matches.

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Texas A&M University

1. Taylor, Kristen Hawkins. Genetic analyses of bovine CARD15, a putative disease resistance gene.

Degree: PhD, Genetics, 2004, Texas A&M University

Through a binding partner the CARD15 gene activates NF-kB, a molecule with a role in the initiation of the inflammatory immune response. The gene is highly conserved in both structure and function in human and mouse and has recently been implicated as a disease resistance gene in Crohn's disease and Blau Syndrome in human. The gene's relationship to disease and its conservation between species suggests that it may also have a conserved role in bovine disease resistance. To elucidate the potential role of bovine CARD15 in disease resistance, the gene was characterized in cattle. Bovine CARD15 is located 4.2 cR5000 telomeric to ADCY7 on chromosome 18. It spans ~30 kb and is comprised of 12 exons, 11 of which are coding. Bovine CARD15 is expressed in many tissues, but is most abundant in peripheral blood leukocytes. An extensive comparative analysis between the bovine, mouse and human CARD15 genes revealed high levels of inter-species conservation in sequence, genomic structure and protein domains. Conserved putative regulatory motifs were identified in the three species comparison of the 5'UTR, 3'UTR and the intronic sequences flanking exons. Additionally, diverse regulatory motifs were identified in each of the species indicating an evolutionary divergence in the mechanisms of regulation of gene expression. To assess the extent of genetic diversity within bovine CARD15, 41 individuals from nine breeds representing two subspecies were sequenced and screened for polymorphisms. Thirty-six single nucleotide polymorphisms (SNPs) were identified including 26 within the gene transcript. Haplotypes were estimated for each individual and parsimonious SNP sets were identified with which the multi-locus Bos taurus and Bos indicus haplotypes may be reconstructed. There was a significantly higher rate of substitutions within Bos indicus than in Bos taurus. A significantly higher rate of nonsynonymous to synonymous substitutions was found in Bos taurus indicating that positive Darwinian selection is acting on the gene within this subspecies. Association analyses were performed between these SNP loci and haplotypes with Johne's disease. No overwhelming evidence for a simple causal relationship was detected. Assays are provided to screen populations of cattle for variation in the CARD15 gene. Advisors/Committee Members: Womack, James E. (advisor), Derr, James N. (committee member), Adams, L. Garry (committee member), Roussel, Allen J. (committee member).

Subjects/Keywords: CARD15; NOD2; bovine; disease resistance; Crohn's; Johne's

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APA (6th Edition):

Taylor, K. H. (2004). Genetic analyses of bovine CARD15, a putative disease resistance gene. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/219

Chicago Manual of Style (16th Edition):

Taylor, Kristen Hawkins. “Genetic analyses of bovine CARD15, a putative disease resistance gene.” 2004. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/219.

MLA Handbook (7th Edition):

Taylor, Kristen Hawkins. “Genetic analyses of bovine CARD15, a putative disease resistance gene.” 2004. Web. 28 Feb 2021.

Vancouver:

Taylor KH. Genetic analyses of bovine CARD15, a putative disease resistance gene. [Internet] [Doctoral dissertation]. Texas A&M University; 2004. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/219.

Council of Science Editors:

Taylor KH. Genetic analyses of bovine CARD15, a putative disease resistance gene. [Doctoral Dissertation]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/219


University of Florida

2. Pinedo, Pablo. Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection.

Degree: PhD, Veterinary Medical Sciences - Veterinary Medicine, 2008, University of Florida

Our first objective (Studies 1-2) was to analyze the association among results of five different diagnostic tests for detection of paratuberculosis infection in cattle. Our second objective (Studies 3-4) was to characterize the distribution of polymorphisms in four immune related genes and test their association with susceptibility to paratuberculosis infection in cattle. In Study 1, results of a serum ELISA, fecal culture, and nested PCR tests on milk, blood, and feces for Mycobacterium paratuberculosis (MAP) detection were analyzed to determine associations and levels of agreement between pairs of tests. The agreement between results was poor, slight and fair in two, five and three of the ten possible combinations. Fecal culture and fecal PCR resulted in the highest kappa coefficient (fair agreement). Combined use of ELISA and fecal PCR has the potential to increase the overall sensitivity for the diagnosis of paratuberculosis. In Study 2, the association between ELISA seroreactivity and MAP presence in milk, as detected by nested PCR was analyzed. An irregular pattern of detection was observed for PCR outcomes along with fluctuations in serial ELISA results. Kappa coefficient indicated a slight agreement between both tests, suggesting that the ability of serum ELISA, as indicator of the likelihood of milk shedding of MAP in dairy cows, is questionable. In Studies 3 and 4, polymorphisms in four candidate genes related to the immune function; caspase recruitment domain 15 gene (CARD15), interferon gamma (BoIFNG), toll-like receptor 4 (TLR4), and solute carrier family 11 member 1 (SLC11A1), were analyzed. Significant differences were found in allelic frequencies between cases and controls for CARD15 SNP2197/C733R, BoIFNG-SNP2781 and SLC11A1 microsatellites. In the analysis of genotypes, a significant association was found between infection status and CARD15 SNP2197/C733R, BoIFNG SNP2781 and SLC11A1-275-279-281 microsatellites. When variables breed and age were included in the multivariate logistic regression analysis, the only statistically significant effect was for CARD15 SNP2197/C733R polymorphism. The estimated odds of infection for heterozygous cows were 3.35 times the odds of infection of cows homozygous for the major genotype. Results suggest a role for CARD15 gene in the susceptibility of cattle to paratuberculosis infection. ( en ) Advisors/Committee Members: Rae, Darrell O. (committee chair), Melendez, Pedro (committee member), Buergelt, Claus-Diet (committee member), Donovan, Gerald A. (committee member), Morel, Laurence (committee member).

Subjects/Keywords: Alleles; Cattle; Diseases; Herds; Infections; Macrophages; Milk; Mycobacterium avium; Paratuberculosis; Polymerase chain reaction; card15, cattle, genetic, ifng, mycobacterium, paratuberculosis, slc11a1, susceptibility, tlr4

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APA (6th Edition):

Pinedo, P. (2008). Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0022315

Chicago Manual of Style (16th Edition):

Pinedo, Pablo. “Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection.” 2008. Doctoral Dissertation, University of Florida. Accessed February 28, 2021. https://ufdc.ufl.edu/UFE0022315.

MLA Handbook (7th Edition):

Pinedo, Pablo. “Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection.” 2008. Web. 28 Feb 2021.

Vancouver:

Pinedo P. Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection. [Internet] [Doctoral dissertation]. University of Florida; 2008. [cited 2021 Feb 28]. Available from: https://ufdc.ufl.edu/UFE0022315.

Council of Science Editors:

Pinedo P. Diagnostic Tools and Genetic Susceptibility Factors Associated with Bovine Paratuberculosis Infection. [Doctoral Dissertation]. University of Florida; 2008. Available from: https://ufdc.ufl.edu/UFE0022315


Kaunas University of Medicine

3. Žvirblienė, Aida. Ligonių, sergančių uždegiminėmis žarnų ligomis, genotipo, fenotipo ir mitybos ypatybės.

Degree: Dissertation, Medicine, 2007, Kaunas University of Medicine

Inflammatory bowel disease (IBD) are multifactorial disorders resulting from the complex interplay between polygenic defects, immunological changes and hitherto unknown enviromental risk factors. The genetic studies are very intensive in the entire world, because the genetically differences between populations decline due to rising migration and racial mixturing. Aim of the study was to identify and evaluate phenotypic, genotypic peculiarities, and nutrition habits of patients with inflammatory bowel disease. 1. To analyze peculiarities of clinical course of patients with inflammatory bowel disease. 2. To identify frequency of genes polymorphisms (for CARD15 gene ARG702Trp (SNP8), Gly908Arg (SNP12), and Leu1007finsC (SNP13) and for two TNF-alfa gene promoter polymorphisms –857C>T (rs1799724) and –308G>A (rs1800629) in patients with inflammatory bowel disease and healthy controls. 3. To evaluate genotype – phenotype association in patients with inflammatory bowel disease. 4. To analyze nutrition habits of patients with inflammatory bowel disease. MATERIALS AND METHODS I. For retrospective analysis of peculiarities of clinical course of patients with ulcerative colitis and Crohn’s disease data of case histories of 273 IBD patients (218 – UC, 55 – CD) referred to the Department of Gastroenterology of Kaunas University of Medicine hospital during 1995–2001 were evaluated. II. For genetic part of the study, a case-control study was performed in the Department of Gastroenterology,... [to full text] Advisors/Committee Members: Kiudelis, Mindaugas (Doctoral dissertation committee member), Kupčinskas, Limas (Doctoral dissertation supervisor), Barauskas, Giedrius (Doctoral dissertation opponent), Sederavičius, Antanas (Doctoral dissertation committee member), Petkevičienė, Janina (Doctoral dissertation committee member), Dadonienė, Jolanta (Doctoral dissertation committee member), Jonaitis, Laimas (Doctoral dissertation committee chair), Petkevičius, Saulius (Doctoral dissertation opponent).

Subjects/Keywords: Case-control study; Lietuvių populiacija. Crohn’s disease; Uždegiminės žarnų ligos; Opinis kolitas; Mityba; TNF-alfa; Diet; TNF-alpha; Inflammatory bowel disease; Lithuanian population; CARD15; Krono liga; Atvejo-kontrolės tyrimas; Ulcerative colitis

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APA (6th Edition):

Žvirblienė, A. (2007). Ligonių, sergančių uždegiminėmis žarnų ligomis, genotipo, fenotipo ir mitybos ypatybės. (Doctoral Dissertation). Kaunas University of Medicine. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2007~D_20070305_145231-14341 ;

Chicago Manual of Style (16th Edition):

Žvirblienė, Aida. “Ligonių, sergančių uždegiminėmis žarnų ligomis, genotipo, fenotipo ir mitybos ypatybės.” 2007. Doctoral Dissertation, Kaunas University of Medicine. Accessed February 28, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2007~D_20070305_145231-14341 ;.

MLA Handbook (7th Edition):

Žvirblienė, Aida. “Ligonių, sergančių uždegiminėmis žarnų ligomis, genotipo, fenotipo ir mitybos ypatybės.” 2007. Web. 28 Feb 2021.

Vancouver:

Žvirblienė A. Ligonių, sergančių uždegiminėmis žarnų ligomis, genotipo, fenotipo ir mitybos ypatybės. [Internet] [Doctoral dissertation]. Kaunas University of Medicine; 2007. [cited 2021 Feb 28]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2007~D_20070305_145231-14341 ;.

Council of Science Editors:

Žvirblienė A. Ligonių, sergančių uždegiminėmis žarnų ligomis, genotipo, fenotipo ir mitybos ypatybės. [Doctoral Dissertation]. Kaunas University of Medicine; 2007. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2007~D_20070305_145231-14341 ;

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