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You searched for subject:(C CP Fibers). Showing records 1 – 3 of 3 total matches.

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Clemson University

1. Straut, Christine. FUNDAMENTAL ASPECTS OF SMALL MOLECULES AND MACROMOLECULES ON POLY(ETHYLENE TEREPHTHALATE) CAPILLARY-CHANNELED POLYMER FIBERS.

Degree: PhD, Chemistry, 2009, Clemson University

Stationary phases and adsorbents are continually being developed to enhance separations in high performance liquid chromatography (HPLC). Polymeric stationary phases have gained popularity due to their ability to be employed over a large pH range and because they are more chemically robust when compared to silica based phases. Capillary-channeled polymer (C-CP) fibers are an alternative to traditional porous packed bed beads. These fibers have a unique geometry with increased the surface area/volume ratios when compared to cylindrical fibers. Very unique characteristics are realized in the use of the C-CP fibers, including drastically reduced backpressures and selection of solute-surface interaction through the use of different base polymers (e.g., polypropylene, polyester, and nylon). They are also non-porous, which alleviates the mass transfer limitations encountered in macromolecular separations. The C-CP fiber columns have been demonstrated to provide efficient separations of proteins under hydrophobic interaction chromatography (HIC), reversed-phase (RP), and ion-exchange chromatography (IEC) conditions. Fundamental studies of the loading characteristics provide a better understanding of how the adsorption and subsequent separation works with the C-CP fibers. These studies will also determine the overall loading or dynamic capacity of the fiber. Frontal analysis (FA) was used to evaluate of the breakthrough curves and reveal the kinetic and thermodynamic properties of the fibers. The fibers maintain kinetic stability at very high linear velocities for both the small molecules and macromolecules studied. These studies further demonstrate the applicability of the C-CP fiber as an adsorbent/stationary phase for liquid chromatography separation. Advisors/Committee Members: Marcus, R. Kenneth, Creager , Stephen, Christensen , Kenneth, Perahia , Devora.

Subjects/Keywords: Adsorption; C-CP; Fibers; Isotherm; Polyethylene terephthalate; Analytical Chemistry

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APA (6th Edition):

Straut, C. (2009). FUNDAMENTAL ASPECTS OF SMALL MOLECULES AND MACROMOLECULES ON POLY(ETHYLENE TEREPHTHALATE) CAPILLARY-CHANNELED POLYMER FIBERS. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/366

Chicago Manual of Style (16th Edition):

Straut, Christine. “FUNDAMENTAL ASPECTS OF SMALL MOLECULES AND MACROMOLECULES ON POLY(ETHYLENE TEREPHTHALATE) CAPILLARY-CHANNELED POLYMER FIBERS.” 2009. Doctoral Dissertation, Clemson University. Accessed January 19, 2021. https://tigerprints.clemson.edu/all_dissertations/366.

MLA Handbook (7th Edition):

Straut, Christine. “FUNDAMENTAL ASPECTS OF SMALL MOLECULES AND MACROMOLECULES ON POLY(ETHYLENE TEREPHTHALATE) CAPILLARY-CHANNELED POLYMER FIBERS.” 2009. Web. 19 Jan 2021.

Vancouver:

Straut C. FUNDAMENTAL ASPECTS OF SMALL MOLECULES AND MACROMOLECULES ON POLY(ETHYLENE TEREPHTHALATE) CAPILLARY-CHANNELED POLYMER FIBERS. [Internet] [Doctoral dissertation]. Clemson University; 2009. [cited 2021 Jan 19]. Available from: https://tigerprints.clemson.edu/all_dissertations/366.

Council of Science Editors:

Straut C. FUNDAMENTAL ASPECTS OF SMALL MOLECULES AND MACROMOLECULES ON POLY(ETHYLENE TEREPHTHALATE) CAPILLARY-CHANNELED POLYMER FIBERS. [Doctoral Dissertation]. Clemson University; 2009. Available from: https://tigerprints.clemson.edu/all_dissertations/366


Clemson University

2. Pittman, Jennifer. SURFACE MODIFICATIONS OF CAPILLARY-CHANNELED POLYMER (C-CP) FIBERS FOR APPLICATIONS IN HIGHLY SELECTIVE SEPARATIONS.

Degree: PhD, Chemistry, 2012, Clemson University

High Performance Liquid Chromatography (HPLC) is a key component in the purification and separation of biological samples. Stationary phases in HPLC are generally silica based porous particles or monoliths designed with high surface area and high capacities in mind. However, in the field of macromolecule separations, specifically proteins, the porous based stationary phases have inherent issues that include slow mass transfer, high operating back pressures, and analyte carryover. Recent research has looked to non-porous polymeric materials as stationary phases in HPLC to overcome these challenges. Specifically, fibrous based polymer stationary phases exhibit significant benefits for macromolecules that include improved mass transfer, decreased operating pressures, and improved chemical robustness. Capillary-Channeled Polymer (C-CP) fibers have been under investigation in the Marcus laboratory for their application as stationary phases in HPLC. C-CP fibers are extruded from standard textile polymers through a spinneret. The spinneret shape provides the unique structure of the fibers, which consists of eight channels that run the length of the fiber. These C-CP fibers have been successfully employed for macromolecule separations due to the increased surface area over cylindrical fibers, an improved mass transfer due to their non-porous nature, and reduced back pressures allowing for operation at higher linear velocities. C-CP fibers come in a variety of base polymers; polyester, polypropylene, and nylon, providing a wide array of chemical interactions (ionic, pi-pi, hydrophobic) and therefore separation mechanisms to occur. However, the ability to generate HPLC stationary phase surfaces with a high degree of analyte specificity is desired. The focus of this research is on modification of C-CP fibers, specifically to generate a high density functional group surface for analyte selective interactions. All three available base polymers of C-CP fibers were evaluated for their ability to undergo chemical modification while maintaining the structural integrity and characteristics of the fibers. Several modification approaches, including plasma grafting, covalent modification, and lipid adsorption, were utilized and their performance evaluated in order to obtain metal or protein selective HPLC stationary phases. Advisors/Committee Members: Marcus, R. Kenneth, Christensen , Kenneth A, Chumanov , George, Pennington , William T.

Subjects/Keywords: Capillary-Channeled Polymer (C-CP) Fibers; High Performance Liquid Chromatography (HPLC); Polymer Stationary Phases; Selective Separations; Solid Phase Extraction (SPE); Surface Modifications; Analytical Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pittman, J. (2012). SURFACE MODIFICATIONS OF CAPILLARY-CHANNELED POLYMER (C-CP) FIBERS FOR APPLICATIONS IN HIGHLY SELECTIVE SEPARATIONS. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/919

Chicago Manual of Style (16th Edition):

Pittman, Jennifer. “SURFACE MODIFICATIONS OF CAPILLARY-CHANNELED POLYMER (C-CP) FIBERS FOR APPLICATIONS IN HIGHLY SELECTIVE SEPARATIONS.” 2012. Doctoral Dissertation, Clemson University. Accessed January 19, 2021. https://tigerprints.clemson.edu/all_dissertations/919.

MLA Handbook (7th Edition):

Pittman, Jennifer. “SURFACE MODIFICATIONS OF CAPILLARY-CHANNELED POLYMER (C-CP) FIBERS FOR APPLICATIONS IN HIGHLY SELECTIVE SEPARATIONS.” 2012. Web. 19 Jan 2021.

Vancouver:

Pittman J. SURFACE MODIFICATIONS OF CAPILLARY-CHANNELED POLYMER (C-CP) FIBERS FOR APPLICATIONS IN HIGHLY SELECTIVE SEPARATIONS. [Internet] [Doctoral dissertation]. Clemson University; 2012. [cited 2021 Jan 19]. Available from: https://tigerprints.clemson.edu/all_dissertations/919.

Council of Science Editors:

Pittman J. SURFACE MODIFICATIONS OF CAPILLARY-CHANNELED POLYMER (C-CP) FIBERS FOR APPLICATIONS IN HIGHLY SELECTIVE SEPARATIONS. [Doctoral Dissertation]. Clemson University; 2012. Available from: https://tigerprints.clemson.edu/all_dissertations/919

3. Haupt-Renaud, Paul. Evaluation of Next Generation Capillary-Channeled Polymer Fibers and the Implementation of C-CP Fiber Modification Modalities on Non-Fiber Substrates.

Degree: MS, Chemistry, 2016, Clemson University

Developing new stationary phases for liquid chromatography is continuing to drive high performance liquid chromatography (HPLC) into the future. In this regard the Marcus’ group has been leveraging the continued advances of Capillary-Channeled Polymer (C-CP) fibers in an attempt to meet the demand of high throughput biomarcomolecule chromatography. Separation mechanisms studied include: ion-exchange (IC), reversed phase (RP), affinity, hydrophobic interaction chromatography (HIC). In this work, the next generation of C-CP fiber stationary phases was thoroughly evaluated with respect to hydrodynamic concerns relating to protein chromatography. Traditionally C-CP fibers have eight channels that run co-linearly along the length of the fiber. Packed C-CP fibers form a network of pseudo-open capillary structures through channels interdigitating. The fibers studied have a much higher surface area to volume ratio compared to circular fibers with similar diameters. The open tubular network has an added bonus of operating at low back pressures. C-CP fibers are non-porous with regards to biomarcomolecules, resulting in fast mass transfer kinetics causing no significant C-term band broadening. The next generation of C-CP fiber has been developed with three larger more ridged channels. This design allows for tighter packing densities without compromising channel integrity. This advancement allows the fibers to operate at higher linear velocities leading to a separation of a six-protein suit (ribonuclease A, cytochrome C, lysozyme, transferrin, bovine serum albumin, and α-chimotrypsinogen) under reversed phase conditions. Surface modification of the C-CP fibers has been accomplished with a variety of techniques, both through covalent and physical adsorption modification. Of particular interest to this work is the Lipid Tethered Ligand (LTL) surface modification modality, which has seen excellent success when employed on polypropylene C-CP fibers. LTLs functionalize a surface with ion-exchange or affinity ligands through hydrophobic physical adsorption to augment the available surface chemistry in a quick and simple flow-through system. In the work presented here, the LTL system was applied to the most commonly used polymer resin, polystyrene-divinylbenzene. The effectiveness of LTL loading, stability, and kinetics on PS-DVB was evaluated. Ligand availability was evaluated with both biotin-LTL for the extraction of streptavidin and iminodiacetic acid-LTL for the extraction of methylene blue. Advisors/Committee Members: Dr. R. Kenneth Marcus, Committee, Chair Dr. Jeffry Anker, Dr. Carlos Garcia.

Subjects/Keywords: C-CP Fibers; Fiber Chromatography; Lipid Tethered Ligands; Surface Modification

…protein chromatography. C-CP fibers are produces with a variety of polymers (polyester… …Clemson University26. Traditionally, C-CP fibers have eight channels that run co-linearly for… …defined by the Van Deemter equation32. This is not the case with C-CP fibers; the fibers have 3… …speed protein separations36. Protein separations have been accomplished on C-CP fibers using a… …limited by what can be extruded into C-CP fibers. Due to this, extensive efforts have been taken… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Haupt-Renaud, P. (2016). Evaluation of Next Generation Capillary-Channeled Polymer Fibers and the Implementation of C-CP Fiber Modification Modalities on Non-Fiber Substrates. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/2343

Chicago Manual of Style (16th Edition):

Haupt-Renaud, Paul. “Evaluation of Next Generation Capillary-Channeled Polymer Fibers and the Implementation of C-CP Fiber Modification Modalities on Non-Fiber Substrates.” 2016. Masters Thesis, Clemson University. Accessed January 19, 2021. https://tigerprints.clemson.edu/all_theses/2343.

MLA Handbook (7th Edition):

Haupt-Renaud, Paul. “Evaluation of Next Generation Capillary-Channeled Polymer Fibers and the Implementation of C-CP Fiber Modification Modalities on Non-Fiber Substrates.” 2016. Web. 19 Jan 2021.

Vancouver:

Haupt-Renaud P. Evaluation of Next Generation Capillary-Channeled Polymer Fibers and the Implementation of C-CP Fiber Modification Modalities on Non-Fiber Substrates. [Internet] [Masters thesis]. Clemson University; 2016. [cited 2021 Jan 19]. Available from: https://tigerprints.clemson.edu/all_theses/2343.

Council of Science Editors:

Haupt-Renaud P. Evaluation of Next Generation Capillary-Channeled Polymer Fibers and the Implementation of C-CP Fiber Modification Modalities on Non-Fiber Substrates. [Masters Thesis]. Clemson University; 2016. Available from: https://tigerprints.clemson.edu/all_theses/2343

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