You searched for subject:(Bone morphogenetic protein 2).
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Wake Forest University
1.
Kowalczewski, Christine Jane.
Natural Polymeric Carriers for Local Delivery of Therapeutic Agents to Promote Enhanced Bone Regeneration.
Degree: 2014, Wake Forest University
URL: http://hdl.handle.net/10339/47453 
► Bone has the innate ability to heal, but on occasion defects surpass a critical size capable of spontaneously healing. These critically-sized bone defects pose significant…
(more)
▼ Bone has the innate ability to heal, but on occasion defects surpass a critical size capable of spontaneously healing. These critically-sized bone defects pose significant challenges for reforming bone continuity and often require surgical intervention to facilitate bone regeneration. Recently, products combining biomaterials with recombinant human bone morphogenetic protein 2 (rhBMP-2) have proven to be a successful clinical alternative to autografts in promoting bone regeneration. While collagen rhBMP-2 carriers have shown robust regenerative capabilities, adverse side effects such as ectopic bone growth are likely caused by a burst release of supraphysiological levels of rhBMP-2 from the carrier. We hypothesize that improved bone regeneration can be achieved through tailored rhBMP-2 release from keratin carriers or through fibrin surface-mediated delivery of siRNA targeting rhBMP-2 antagonists. For this reason, we developed an alternative natural polymer rhBMP-2 carrier from keratin. Keratin can be formed into a number of biomaterials which have advantages over collagen including controlled rates of degradation, flexible material properties, and tunable rates of rhBMP-2 delivery. In a critically-sized rat mandibular model the keratin biomaterials reduced ectopic bone growth, and achieved comparable levels of bone healing to existing collagen-based clinical alternative for craniofacial injuries. However, our keratin carriers still delivered a supraphysiological concentration of rhBMP-2. We have separately observed the ability of small interfering RNA (siRNA) delivered from fibrin carriers to knockdown myelin-associated glycoprotein (a molecular inhibitor to nerve regeneration). We therefore attempted to apply the same approach to bone regeneration. In order to lower the therapeutic rhBMP-2 dose, targeting rhBMP-2 antagonists such as noggin through substrate-mediated delivery of siRNA complexes can potentially increase local rhBMP-2 efficacy. Fibrin, a natural polymer involved in bone healing, is able to electrostatically bind siRNA complexed with polycationic transfection agents to its surface in order to locally control molecular inhibitors of bone regeneration. Sustained transfection of sequence specific siRNA targeting noggin was able to achieve dose-dependent reduction of noggin mRNA levels after administering a supraphysiological dose of rhBMP-2. In conclusion, regeneration of critically-sized bone defects can be enhanced through the delivery of therapeutic agents to control local molecular cues from natural polymers.
Subjects/Keywords: Bone Morphogenetic Protein -2
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APA (6th Edition):
Kowalczewski, C. J. (2014). Natural Polymeric Carriers for Local Delivery of Therapeutic Agents to Promote Enhanced Bone Regeneration. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/47453
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kowalczewski, Christine Jane. “Natural Polymeric Carriers for Local Delivery of Therapeutic Agents to Promote Enhanced Bone Regeneration.” 2014. Thesis, Wake Forest University. Accessed March 08, 2021.
http://hdl.handle.net/10339/47453.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kowalczewski, Christine Jane. “Natural Polymeric Carriers for Local Delivery of Therapeutic Agents to Promote Enhanced Bone Regeneration.” 2014. Web. 08 Mar 2021.
Vancouver:
Kowalczewski CJ. Natural Polymeric Carriers for Local Delivery of Therapeutic Agents to Promote Enhanced Bone Regeneration. [Internet] [Thesis]. Wake Forest University; 2014. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10339/47453.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kowalczewski CJ. Natural Polymeric Carriers for Local Delivery of Therapeutic Agents to Promote Enhanced Bone Regeneration. [Thesis]. Wake Forest University; 2014. Available from: http://hdl.handle.net/10339/47453
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Boston University
2.
Femia, Alexandra Lynn.
The comparative role of demineralized bone matrix placement on the periosteum versus in the muscle with and without bone morphogenetic protein 2.
Degree: MS, Medical Sciences, 2015, Boston University
URL: http://hdl.handle.net/2144/16185
► Demineralized bone matrix (DBM) is an allograft material used in orthopedics that promotes endochondral bone formation. While the placement of DBM on either the periosteal…
(more)
▼ Demineralized bone matrix (DBM) is an allograft material used in orthopedics that promotes endochondral bone formation. While the placement of DBM on either the periosteal surface of a bone or within a skeletal muscle promotes the recruitment of stem cells that can form skeletal tissues through the temporal progression of endochondral bone development, it remains unclear to what degree these processes are different between the two sites. In this study, we utilize a comparative in vivo model of endochondral ossification by implanting the DBM on the periosteum and in the muscle. Within the muscle we further compared the effects of DBM with and without Bone morphogenetic protein-2 (BMP-2), a primary morphogenetic factor involved in the differentiation of skeletal stem cells. The mice were harvested at various time points after DBM implantation in order to analyze the development of the bone. Analysis included X-ray imaging, microCT imaging, and mRNA expression. Plain x-ray and micro-CT imaging analysis showed mineralized bone formation in the implant on the periosteum and in the muscle with BMP-2, but no growth in the muscle when BMP-2 was not added to the DBM. The mechanisms for bone development were further analyzed by qRT-PCR to determine temporal patterns and levels of expression of various stem cell and differentiated skeletal cell associated genes. The stem cell gene expression varied between implant placement locations suggesting different mechanisms for stem cell recruitment. Interesting, while DBM implants in the muscle without BMP did not induce mineralized tissue specific mRNA expression; specific stem cell and early skeletal cell lineage commitment genes were present. These results suggest that while DBM in muscle is capable of recruiting stem cells that higher BMP-2 levels are needed to promote the progression of cartilage to mineralized bone in muscle tissues.
Subjects/Keywords: Medicine; Bone morphogenetic protein; Bone morphogenetic protein 2; Demineralized bone matrix; Orthopedic surgery
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APA (6th Edition):
Femia, A. L. (2015). The comparative role of demineralized bone matrix placement on the periosteum versus in the muscle with and without bone morphogenetic protein 2. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/16185
Chicago Manual of Style (16th Edition):
Femia, Alexandra Lynn. “The comparative role of demineralized bone matrix placement on the periosteum versus in the muscle with and without bone morphogenetic protein 2.” 2015. Masters Thesis, Boston University. Accessed March 08, 2021.
http://hdl.handle.net/2144/16185.
MLA Handbook (7th Edition):
Femia, Alexandra Lynn. “The comparative role of demineralized bone matrix placement on the periosteum versus in the muscle with and without bone morphogenetic protein 2.” 2015. Web. 08 Mar 2021.
Vancouver:
Femia AL. The comparative role of demineralized bone matrix placement on the periosteum versus in the muscle with and without bone morphogenetic protein 2. [Internet] [Masters thesis]. Boston University; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2144/16185.
Council of Science Editors:
Femia AL. The comparative role of demineralized bone matrix placement on the periosteum versus in the muscle with and without bone morphogenetic protein 2. [Masters Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/16185
Georgia Tech
3.
Priddy, Lauren B.
Biomaterial strategies for improved bone healing with bone morphogenetic protein-2 delivery.
Degree: PhD, Biomedical Engineering (Joint GT/Emory Department), 2015, Georgia Tech
URL: http://hdl.handle.net/1853/55531
► This thesis investigated hybrid biomaterial systems with controlled strategies for bone morphogenetic protein-2 (BMP-2) delivery to promote structural and functional restoration of segmental bone defects.…
(more)
▼ This thesis investigated hybrid biomaterial systems with controlled strategies for
bone morphogenetic protein-
2 (BMP-
2) delivery to promote structural and functional restoration of segmental
bone defects. Using a critically sized rat segmental
bone defect model, we (i) evaluated the effects of alginate hydrogel oxidation on BMP-
2 release and
bone regeneration, (ii) elucidated the spatiotemporal effects of high dose BMP-
2 on
bone healing and gene expression, and (iii) investigated the ability of amniotic membrane to attenuate heterotopic mineralization in critically sized
bone defects. Importantly, our rat model recapitulated adverse effects observed clinically with orthotopic high dose BMP-
2 delivery, specifically heterotopic mineralization, prolonged local inflammation, and systemic inflammatory effects. By identifying specific alterations in gene expression as a function of time and BMP-
2 dose, this thesis contributes to our understanding of the complex process of
bone healing during the early stages of large
bone defect regeneration. Although the hybrid alginate-PCL delivery system did not reduce heterotopic ossification with high dose BMP-
2, extracellular matrix-derived amniotic membrane surrounding collagen sponge scaffolds resulted in less heterotopic mineralization compared to collagen sponge alone, which motivates the accelerated translation of amniotic membrane for
bone regeneration applications. The findings here support the overall hypothesis that a biomaterial delivery vehicle that allows for localized growth factor availability and minimal heterotopic
bone formation would facilitate structural and functional restoration of segmental
bone defects. By considering these fundamental biomaterial parameters, we may more effectively harness endogenous repair mechanisms for successful
bone regeneration.
Advisors/Committee Members: Guldberg, Robert E (advisor).
Subjects/Keywords: Bone regeneration; Bone morphogenetic protein-2 (BMP-2); Heterotopic mineralization; Biomaterials
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APA ·
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Manager
APA (6th Edition):
Priddy, L. B. (2015). Biomaterial strategies for improved bone healing with bone morphogenetic protein-2 delivery. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/55531
Chicago Manual of Style (16th Edition):
Priddy, Lauren B. “Biomaterial strategies for improved bone healing with bone morphogenetic protein-2 delivery.” 2015. Doctoral Dissertation, Georgia Tech. Accessed March 08, 2021.
http://hdl.handle.net/1853/55531.
MLA Handbook (7th Edition):
Priddy, Lauren B. “Biomaterial strategies for improved bone healing with bone morphogenetic protein-2 delivery.” 2015. Web. 08 Mar 2021.
Vancouver:
Priddy LB. Biomaterial strategies for improved bone healing with bone morphogenetic protein-2 delivery. [Internet] [Doctoral dissertation]. Georgia Tech; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1853/55531.
Council of Science Editors:
Priddy LB. Biomaterial strategies for improved bone healing with bone morphogenetic protein-2 delivery. [Doctoral Dissertation]. Georgia Tech; 2015. Available from: http://hdl.handle.net/1853/55531
University of Alberta
4.
Chen, Chao.
Effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow mesenchymal stem
cells.
Degree: MS, Department of Surgery, 2011, University of Alberta
URL: https://era.library.ualberta.ca/files/w9505192t
► The osteogenic effects of bone morphogenetic protein-2 (BMP-2) on human mesenchymal stem cells (MSCs) are less profound than expected as compared with rodent cells, and…
(more)
▼ The osteogenic effects of bone morphogenetic protein-2
(BMP-2) on human mesenchymal stem cells (MSCs) are less profound
than expected as compared with rodent cells, and supraphysiological
dose of BMP-2 is required to achieve desired clinical outcome. The
mechanism for this phenomenon is unclear. In this study, we
examined the effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow MSCs in vitro. Our
data show that macrophage conditioned medium significantly
decreased the migration capacity, metabolic activity and
BMP-2-induced osteogenesis of MSCs. In addition, knocking down
noggin by small interfering RNA (siRNA) also significantly
decreased BMP-2-induced osteogenesis and proliferation of MSCs. In
summary, our studies demonstrated that macrophages and knocking
down the expression of noggin decreased BMP-2-induced osteogenesis
of human MSCs in vitro. In the future, manipulation on macrophage
activation and noggin expression may allow us to achieve higher
BMP-2-induced osteogenesis that leads to better bone
healing.
Subjects/Keywords: bone morphogenetic protein-2; mesenchymal stem cells; macrophages; noggin; bone; osteogenesis
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APA ·
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APA (6th Edition):
Chen, C. (2011). Effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow mesenchymal stem
cells. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/w9505192t
Chicago Manual of Style (16th Edition):
Chen, Chao. “Effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow mesenchymal stem
cells.” 2011. Masters Thesis, University of Alberta. Accessed March 08, 2021.
https://era.library.ualberta.ca/files/w9505192t.
MLA Handbook (7th Edition):
Chen, Chao. “Effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow mesenchymal stem
cells.” 2011. Web. 08 Mar 2021.
Vancouver:
Chen C. Effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow mesenchymal stem
cells. [Internet] [Masters thesis]. University of Alberta; 2011. [cited 2021 Mar 08].
Available from: https://era.library.ualberta.ca/files/w9505192t.
Council of Science Editors:
Chen C. Effects of macrophages and noggin suppression on the
BMP-2-induced osteogenesis of human bone marrow mesenchymal stem
cells. [Masters Thesis]. University of Alberta; 2011. Available from: https://era.library.ualberta.ca/files/w9505192t
University of Alberta
5.
Wang, guilin.
Bisphosphonate-modified nanoparticles as drug delivery
systems for bone diseases.
Degree: PhD, Department of Chemical and Materials
Engineering, 2011, University of Alberta
URL: https://era.library.ualberta.ca/files/1544bp45j
► The objective of this thesis is to design nanoparticle (NP)-based drug delivery systems suitable for treatment of bone diseases. Two types of nanocarriers, (1) polymer…
(more)
▼ The objective of this thesis is to design nanoparticle
(NP)-based drug delivery systems suitable for treatment of bone
diseases. Two types of nanocarriers, (1) polymer coated bovine
serum albumin (BSA) NPs and (2) lipid based NPs (micelles and
liposomes) were investigated. The BSA NPs were prepared by a
coacervation method and stabilized with a polymer coating approach.
For bone-specific delivery of bone morphogenetic protein-2 (BMP-2),
a copolymer polyethyleneimine-graft-poly(ethylene glycol)
conjugated with
2-(3-mercaptopropylsulfanyl)-ethyl-1,1-bisphosphonic acid
(PEI-PEG-thiolBP) was synthesized and used for coating the BSA NPs.
The particle size and ζ-potential of the NPs could be effectively
modulated by the processing parameters. All the NPs showed no or
low cytotoxicity (except for a high concentration of PEI), and the
NP encapsulated BMP-2 displayed full retention of its bioactivity.
By encapsulating 125I-labeled BMP-2, the polymer-coated NPs were
assessed for hydroxyapatite (HA) affinity; all NP-encapsulated
BMP-2 showed significant affinity to HA as compared with free BMP-2
in vitro, and the PEI-PEG-thiolBP coated NPs improved the in vivo
retention of BMP-2 compared with uncoated NPs. However, the
biodistribution of NPs after intravenous injection in a rat model
indicated no beneficial effects of thiolBP-coated NPs for bone
targeting. Alternatively, micelles and liposomes were prepared with
a conjugate of distearoylphosphoethanolamine-polyethyleneglycol
with thiolBP (DSPE-PEG-thiolBP) to create mineral-binding
nanocarriers. The thiolBP-decorated liposomes also displayed a
stronger binding affinity to HA and a collagen/HA (Col/HA) scaffold
and gave increased retention in the scaffold in a subcutaneous
implant model in rats. Taking advantage of the high HA affinity of
the BP-liposomes, a sustainable release system was developed by
sequestering the liposomal drugs in the Col/HA scaffolds. Three
different model drugs, carboxyfluorescein, doxorubicin and
lysozyme, were used to evaluate the drug release profiles from the
liposome-loaded scaffolds, and all showed a slowing effect of the
BP on the release of the liposome-encapsulated drugs from the
Col/HA scaffolds. This liposome-scaffold combination will provide a
platform for the application of various therapeutic agents for bone
regeneration. In conclusion, the BP-modified NPs showed strong
mineral-binding affinity. Although the systemic bone targeting was
limited by physiological barriers, these NPs are promising in local
delivery and controlled release of bioactive molecules for
treatment of bone diseases.
Subjects/Keywords: liposome; bone morphogenetic protein-2; nanoparticles; bone targeting; bisphosphonate; drug delivery
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APA ·
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Manager
APA (6th Edition):
Wang, g. (2011). Bisphosphonate-modified nanoparticles as drug delivery
systems for bone diseases. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/1544bp45j
Chicago Manual of Style (16th Edition):
Wang, guilin. “Bisphosphonate-modified nanoparticles as drug delivery
systems for bone diseases.” 2011. Doctoral Dissertation, University of Alberta. Accessed March 08, 2021.
https://era.library.ualberta.ca/files/1544bp45j.
MLA Handbook (7th Edition):
Wang, guilin. “Bisphosphonate-modified nanoparticles as drug delivery
systems for bone diseases.” 2011. Web. 08 Mar 2021.
Vancouver:
Wang g. Bisphosphonate-modified nanoparticles as drug delivery
systems for bone diseases. [Internet] [Doctoral dissertation]. University of Alberta; 2011. [cited 2021 Mar 08].
Available from: https://era.library.ualberta.ca/files/1544bp45j.
Council of Science Editors:
Wang g. Bisphosphonate-modified nanoparticles as drug delivery
systems for bone diseases. [Doctoral Dissertation]. University of Alberta; 2011. Available from: https://era.library.ualberta.ca/files/1544bp45j
Georgia Tech
6.
Hettiaratchi, Marian Hirushika.
Heparin microparticle-mediated delivery of BMP-2 and pluripotent stem cell morphogens for bone repair.
Degree: PhD, Biomedical Engineering (Joint GT/Emory Department), 2016, Georgia Tech
URL: http://hdl.handle.net/1853/59144
► The delivery of bone morphogenetic protein-2 (BMP-2) offers a promising means of stimulating endogenous repair mechanisms to heal severe bone injuries. However, clinical application of…
(more)
▼ The delivery of
bone morphogenetic protein-
2 (BMP-
2) offers a promising means of stimulating endogenous repair mechanisms to heal severe
bone injuries. However, clinical application of growth factor therapy is hindered by the lack of adequate biomaterials to localize BMP-
2 delivery. Glycosaminoglycans, such as heparin, have the capacity to strongly bind BMP-
2 and other growth factors implicated in
bone regeneration, and present the opportunity to locally deliver growth factors to injury sites. Moreover, pluripotent stem cells (PSCs) secrete many potent heparin-binding growth factors that have been implicated in tissue regeneration following cell transplantation and may provide cues for repair. Thus, heparin can also be used to concentrate and deliver PSC-derived morphogens to tissue injury sites, thereby overcoming challenges associated with PSC transplantation. The goal of this work was to improve growth factor delivery for
bone repair by both (1) creating an effective biomaterial for BMP-
2 delivery and (
2) investigating PSC morphogens as a novel source of therapeutic growth factors. We developed heparin-based microparticles that could bind and retain large amounts of bioactive BMP-
2 in vitro and improve BMP-
2 retention in vivo, resulting in spatially localized
bone formation in a critically sized rat femoral defect. Furthermore, heparin microparticles could also sequester and concentrate complex mixtures of bioactive PSC-secreted proteins, which may be developed into cell-free therapies in the future. Overall, this work broadens current understanding of
bone tissue engineering, biomaterial delivery strategies, and stem cell-based therapeutics, and provides valuable insight into developing affinity-based biomaterials for clinical applications.
Advisors/Committee Members: Guldberg, Robert E. (advisor), McDevitt, Todd C. (advisor), Boden, Scott D. (committee member), Botchwey, Edward A. (committee member), García, Andrés J. (committee member), Temenoff, Johnna S. (committee member).
Subjects/Keywords: Heparin microparticles; Bone morphogenetic protein-2; Embryonic stem cells; Bone repair
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APA (6th Edition):
Hettiaratchi, M. H. (2016). Heparin microparticle-mediated delivery of BMP-2 and pluripotent stem cell morphogens for bone repair. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/59144
Chicago Manual of Style (16th Edition):
Hettiaratchi, Marian Hirushika. “Heparin microparticle-mediated delivery of BMP-2 and pluripotent stem cell morphogens for bone repair.” 2016. Doctoral Dissertation, Georgia Tech. Accessed March 08, 2021.
http://hdl.handle.net/1853/59144.
MLA Handbook (7th Edition):
Hettiaratchi, Marian Hirushika. “Heparin microparticle-mediated delivery of BMP-2 and pluripotent stem cell morphogens for bone repair.” 2016. Web. 08 Mar 2021.
Vancouver:
Hettiaratchi MH. Heparin microparticle-mediated delivery of BMP-2 and pluripotent stem cell morphogens for bone repair. [Internet] [Doctoral dissertation]. Georgia Tech; 2016. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1853/59144.
Council of Science Editors:
Hettiaratchi MH. Heparin microparticle-mediated delivery of BMP-2 and pluripotent stem cell morphogens for bone repair. [Doctoral Dissertation]. Georgia Tech; 2016. Available from: http://hdl.handle.net/1853/59144
Erasmus University Rotterdam
7.
Z.D. Mumcuoglu Guvenc (Didem).
Development of an injectable slow release system for bone morphogenetic protein-2.
Degree: 2018, Erasmus University Rotterdam
URL: http://hdl.handle.net/1765/105787
► In this thesis, development of a bone regeneration therapy using biomaterials and growth factors is described. Initially, collagen I based recombinant protein (RCP) microspheres were…
(more)
▼ In this thesis, development of a bone regeneration therapy using biomaterials and growth factors is described. Initially, collagen I based recombinant protein (RCP) microspheres were developed for BMP-2 delivery. Among several parameters investigated, size and crosslinking of microspheres affected the BMP-2 release. To improve bone formation, we have combined microspheres with in-situ gelling hydrogels. Two types of alginate, high mannuronate (SLM) and high guluronate (SLG), and one type of thermosensitive hyaluronic acid were developed for this study. These hydrogels were designed not only to deliver and keep the microspheres at the site but also to act as a scaffold for the regenerating tissue and to fill a defect. The SLG alginate-RCP microspheres formulation was selected for further studies in ectopic and orthotopic bone formation models in rats. First, the effect of BMP-2 dose delivered was tested in the ectopic bone model using 10, 3, 1, 0.3 and 0 μg BMP-2 per implant. In a subsequent calvarial defect experiment, 50 μg/mL and 5 μg/mL BMP-2 containing hydrogels were tested. This study showed that the effective concentration of BMP-2 to induce both ectopic and orthotopic bone is optimally between 15-50 μg/mL.
Subjects/Keywords: Bone morphogenetic protein-2; slow release; recombinant collagen; microspheres; bone regeneration
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
(Didem), Z. M. G. (2018). Development of an injectable slow release system for bone morphogenetic protein-2. (Thesis). Erasmus University Rotterdam. Retrieved from http://hdl.handle.net/1765/105787
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
(Didem), Z.D. Mumcuoglu Guvenc. “Development of an injectable slow release system for bone morphogenetic protein-2.” 2018. Thesis, Erasmus University Rotterdam. Accessed March 08, 2021.
http://hdl.handle.net/1765/105787.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
(Didem), Z.D. Mumcuoglu Guvenc. “Development of an injectable slow release system for bone morphogenetic protein-2.” 2018. Web. 08 Mar 2021.
Vancouver:
(Didem) ZMG. Development of an injectable slow release system for bone morphogenetic protein-2. [Internet] [Thesis]. Erasmus University Rotterdam; 2018. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1765/105787.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
(Didem) ZMG. Development of an injectable slow release system for bone morphogenetic protein-2. [Thesis]. Erasmus University Rotterdam; 2018. Available from: http://hdl.handle.net/1765/105787
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
8.
Polak, Samantha J.
Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds.
Degree: MS, 0408, 2011, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/18409
► Osteoinductive agents, such as BMP-2, are known to improve bone formation when combined with scaffolds. Microporosity (<20??m) has also been shown to influence bone regeneration…
(more)
▼ Osteoinductive agents, such as BMP-
2, are known to improve
bone formation when combined with scaffolds. Microporosity (<20??m) has also been shown to influence
bone regeneration in calcium phosphate (CaP) scaffolds. However, many studies use only the term ???osteoconductive??? to describe the effects of BMP-
2 and microporosity on
bone formation, and do not assess the degree of healing that occurred. The objective of this study was to quantify the influence of BMP-
2 and microporosity on
bone regeneration and healing in biphasic calcium phosphate (BCP) scaffolds using multiple measures including
bone volume fraction (BF), radial distribution (RBD), and specific surface area (SSA). These measures were quantitatively compared by analyzing micro-computed tomography data and used to formally define and assess healing. An accurate custom image segmentation program was developed that utilizes grayscale values, the periodicity of the scaffold, and 3D spatial features to segment background,
bone, and scaffold pixels. This program was used to segment >100 samples, with 900 images each, that were implanted in porcine mandibular defects for 3, 6, 12, and 24 weeks. The assessment of healing presented in this work demonstrates the level of detail possible in evaluating scaffold-guided
bone regeneration. The analysis shows that BMP-
2 and microporosity accelerate healing up to four-fold. BMP-
2 and microporosity were shown to have different and complementary roles in
bone formation that effect the time needed for a defect to heal.
Advisors/Committee Members: Wagoner Johnson, Amy J. (advisor).
Subjects/Keywords: Hydroxyapatite; Bone scaffold; Bone morphogenetic protein 2 (BMP-2); Microporosity; Micro computed tomography (Micro-CT)
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Polak, S. J. (2011). Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/18409
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Polak, Samantha J. “Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds.” 2011. Thesis, University of Illinois – Urbana-Champaign. Accessed March 08, 2021.
http://hdl.handle.net/2142/18409.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Polak, Samantha J. “Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds.” 2011. Web. 08 Mar 2021.
Vancouver:
Polak SJ. Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2011. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2142/18409.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Polak SJ. Quantitative image analysis of in vivo microstructure and growth factor effects in hydroxyapatite bone scaffolds. [Thesis]. University of Illinois – Urbana-Champaign; 2011. Available from: http://hdl.handle.net/2142/18409
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
紅林, 奈央子.
Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts : C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御.
Degree: 博士(歯学), 2014, Hokkaido University / 北海道大学
URL: http://hdl.handle.net/2115/56295
;
http://dx.doi.org/10.14943/doctoral.k11268
► The neuropeptide Y(NPY) system is known as one of the major neural signaling pathways. NPY, produced by peripheral tissues including osteoblasts, is known to bind…
(more)
▼ The neuropeptide Y(NPY) system is known as one of the major neural signaling pathways. NPY, produced by peripheral tissues including osteoblasts, is known to bind to the Y1 receptor. Recently, osteoblast-specific Y1 receptor knockout mice were developed and were found to have a high bone mass phenotype, indicating a role for the NPY-Y1 receptor axis as a regulator of bone homeostasis. However, regulation of Y1 receptor expression during osteoblastic differentiation remains unexplored. In the present study, we examined the role of bone morphogenetic protein (BMP) 2 signaling in regulating Y1 receptor expression. In C2C12 cells, expression of Y1 receptor mRNA was induced by BMP2. This induction was also observed after co-transfection with Smad1 and Smad4, the intracellular signaling molecules of the BMP2 signaling pathway. In a transfection assay, Smad1/4 up-regulated transcriptional activity through interaction with the Y1 receptor gene promoter. Following transfection of MC3T3-E1 cells with siRNA for the Y1 receptor, the expression of alkaline phosphatase, osteocalcin, Runx2 and osterix were increased. These results show that BMP2 signaling regulates Y1 receptor gene expression, and raises the possibility that NPY acts in osteo blasts via an autocrine mechanism.
24
Hokkaido University（北海道大学）. 博士(歯学)
Subjects/Keywords: Bone morphogenetic protein 2; Neuropeptide Y; Osteoblasts; Y1 receptor
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MLA ·
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APA (6th Edition):
紅林, . (2014). Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts : C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御. (Thesis). Hokkaido University / 北海道大学. Retrieved from http://hdl.handle.net/2115/56295 ; http://dx.doi.org/10.14943/doctoral.k11268
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
紅林, 奈央子. “Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts : C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御.” 2014. Thesis, Hokkaido University / 北海道大学. Accessed March 08, 2021.
http://hdl.handle.net/2115/56295 ; http://dx.doi.org/10.14943/doctoral.k11268.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
紅林, 奈央子. “Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts : C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御.” 2014. Web. 08 Mar 2021.
Vancouver:
紅林 . Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts : C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御. [Internet] [Thesis]. Hokkaido University / 北海道大学; 2014. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2115/56295 ; http://dx.doi.org/10.14943/doctoral.k11268.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
紅林 . Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts : C2C12筋芽細胞におけるNeuropeptide Y Y1受容体のBMP2による発現制御. [Thesis]. Hokkaido University / 北海道大学; 2014. Available from: http://hdl.handle.net/2115/56295 ; http://dx.doi.org/10.14943/doctoral.k11268
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Boston University
10.
Cheng, Thomas Wen-Tao.
The role of bone morphogenetic protein 2 in SMA-directed angiogenesis during distraction osteogenesis.
Degree: MS, Medical Sciences, 2015, Boston University
URL: http://hdl.handle.net/2144/16261
► Bone is one of the few organs capable of regeneration after a substantial injury. As the bone heals itself after trauma, the coupling of angiogenesis…
(more)
▼ Bone is one of the few organs capable of regeneration after a substantial injury. As the bone heals itself after trauma, the coupling of angiogenesis to osteogenesis is crucial for the restoration of the skeletal tissue. In prior studies we have shown that Bone Morphogenetic Protein 2 (BMP2), a potent agonist for skeletal formation is expressed by vessels making it a prime candidate that links the morphogenesis of the two tissues. To investigate the role of BMP2 in the coordination of vessel and bone formation, we used a tamoxifen inducible Smooth Muscle Actin (SMA) promoter that conditionally expresses Cre recombinases crossed with a BMP2 floxed mouse in order to conditionally delete the BMP2 gene in smooth muscle actin (SMA) expressing cells. Using the mouse femur as our model for bone regeneration, we performed a surgical technique called distraction osteogenesis (DO) where an osteotomy is created followed by distraction or a gradual separation of the two pieces of bone. This primarily promotes intramembranous ossification at the osteotomy site by mechanical stimulation. Tamoxifen treatment started at day 6 and continued throughout the experiment. At post-operative days 3, 7, 12, 17, 24, and 31, we analyzed the bone and vessel formation by plain X-ray, micro-computed tomography (µCT) and vascular contrast enhanced µCT, and quantitative polymerase chain reaction (qPCR) of selective genes. We assessed both the femur and surrounding tissue to obtain qualitative and quantitative assessments for skeletal and vascular formation. Our results demonstrated that the deletion of BMP2 in vascular tissue resulted in a reduction of angiogenesis in vivo followed by a decrease in skeletal tissue development.
Subjects/Keywords: Medicine; Angiogenesis; Bone morphogenetic protein 2; Osteogenesis; SMA; Bone repair; Distraction osteogenesis
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cheng, T. W. (2015). The role of bone morphogenetic protein 2 in SMA-directed angiogenesis during distraction osteogenesis. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/16261
Chicago Manual of Style (16th Edition):
Cheng, Thomas Wen-Tao. “The role of bone morphogenetic protein 2 in SMA-directed angiogenesis during distraction osteogenesis.” 2015. Masters Thesis, Boston University. Accessed March 08, 2021.
http://hdl.handle.net/2144/16261.
MLA Handbook (7th Edition):
Cheng, Thomas Wen-Tao. “The role of bone morphogenetic protein 2 in SMA-directed angiogenesis during distraction osteogenesis.” 2015. Web. 08 Mar 2021.
Vancouver:
Cheng TW. The role of bone morphogenetic protein 2 in SMA-directed angiogenesis during distraction osteogenesis. [Internet] [Masters thesis]. Boston University; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2144/16261.
Council of Science Editors:
Cheng TW. The role of bone morphogenetic protein 2 in SMA-directed angiogenesis during distraction osteogenesis. [Masters Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/16261
University of Pretoria
11.
[No author].
Physico-chemical modification of kafirin
microstructures for application as biomaterials
.
Degree: 2013, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-11222012-135328/
► Microparticles produced from kafirin, the sorghum grain prolamin protein, by molecular selfassembly using coacervation with acetic acid solvent are vacuolated. They have shown considerable potential…
(more)
▼ Microparticles produced from kafirin, the sorghum
grain prolamin
protein, by molecular selfassembly using
coacervation with acetic acid solvent are vacuolated. They have
shown considerable potential for encapsulation of antioxidants and
for preparation of high quality free-standing bioplastic films.
However, the functional quality of these kafirin microstructures
needs to be improved to exploit their potential application,
particularly as biomaterials. Wet heat, transglutaminase and
glutaraldehyde treatments were used to modify the physical
structure and chemical properties of the kafirin microstructures.
Heat treatment (50–96°C) increased microparticle average size by up
to four-fold to ≈20 μm, probably due to disulphide cross-linking of
kafirin proteins. The vacuoles within these microparticles enlarged
up to >10-fold, probably due to greater expansion of air within
the microparticles with higher temperature, as the vacuoles are
probably footprints of air bubbles. As with heat treatment,
glutaraldehyde (10–30%) treatment resulted in oval microparticles,
up to about four-fold larger than the control, probably due to
covalent glutaraldehyde-polypeptide linkage. Transglutaminase
(0.1–0.6%) treatment had only slight effect on the size and shape
of microparticles, probably because kafirin has very low lysine
content, inhibiting transglutaminase-catalysed cross-linking
through ε-(-glutamyl)-lysine bonding. Surface morphology using
atomic force microscopy indicated that the microparticles
apparently comprised coalesced nanostructures. With heat and
transglutaminase treatments, the microparticles seemed to be
composed of round nanostructures that coalesced into random
irregular shapes, indicative of non-linear
protein aggregation. In
contrast, with glutaraldehyde treatment, the nanostructures were
spindle-shaped and had a unidirectional orientation, probably due
to linear alignment of the nanostructures controlled by
glutaraldehyde-polypeptide linkage. Thin (<50 μm) films prepared
from kafirin microparticles and conventional cast kafirin films
were compared in terms of their water stability and other related
properties. Films cast from microparticles were more water-stable
compared to conventional kafirin films, probably because the large
vacuoles within the kafirin microparticles may have enhanced
protein solubility in the casting solution, thereby improving the
film matrix cohesion. The films prepared from microparticles
treated with glutaraldehyde were more water-stable compared to the
control, despite the loss of plasticizer, probably due to formation
of the covalent glutaraldehyde-polypeptide linkages. The potential
of modified kafirin microparticles to bind
bone morphogenetic
protein-
2 (BMP-
2) was investigated. Compared to a collagen
standard, the BMP-
2 binding capacity of control, heat-treated,
transglutaminase-treated and glutaraldehyde-treated kafirin
microparticles were 7%, 18%, 34% and 22% higher, respectively,
probably mainly due to the vacuoles within the microparticles
creating greater binding surface area.…
Advisors/Committee Members: Prof J R N Taylor (advisor), Dr J Taylor (advisor), Prof V Naidoo (advisor).
Subjects/Keywords: Binding;
Bone morphogenetic protein-2 (bmp-2);
Water stability;
Cross-linking;
Kafirin;
Microparticles;
UCTD
Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2013). Physico-chemical modification of kafirin
microstructures for application as biomaterials
. (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-11222012-135328/
Chicago Manual of Style (16th Edition):
author], [No. “Physico-chemical modification of kafirin
microstructures for application as biomaterials
.” 2013. Doctoral Dissertation, University of Pretoria. Accessed March 08, 2021.
http://upetd.up.ac.za/thesis/available/etd-11222012-135328/.
MLA Handbook (7th Edition):
author], [No. “Physico-chemical modification of kafirin
microstructures for application as biomaterials
.” 2013. Web. 08 Mar 2021.
Vancouver:
author] [. Physico-chemical modification of kafirin
microstructures for application as biomaterials
. [Internet] [Doctoral dissertation]. University of Pretoria; 2013. [cited 2021 Mar 08].
Available from: http://upetd.up.ac.za/thesis/available/etd-11222012-135328/.
Council of Science Editors:
author] [. Physico-chemical modification of kafirin
microstructures for application as biomaterials
. [Doctoral Dissertation]. University of Pretoria; 2013. Available from: http://upetd.up.ac.za/thesis/available/etd-11222012-135328/
University of Pretoria
12.
Anyango, Joseph Ochieng.
Physico-chemical
modification of kafirin microstructures for application as
biomaterials.
Degree: Food Science, 2012, University of Pretoria
URL: http://hdl.handle.net/2263/29708
► Microparticles produced from kafirin, the sorghum grain prolamin protein, by molecular selfassembly using coacervation with acetic acid solvent are vacuolated. They have shown considerable potential…
(more)
▼ Microparticles produced from kafirin, the sorghum grain
prolamin
protein, by molecular selfassembly using coacervation with
acetic acid solvent are vacuolated. They have shown considerable
potential for encapsulation of antioxidants and for preparation of
high quality free-standing bioplastic films. However, the
functional quality of these kafirin microstructures needs to be
improved to exploit their potential application, particularly as
biomaterials. Wet heat, transglutaminase and glutaraldehyde
treatments were used to modify the physical structure and chemical
properties of the kafirin microstructures. Heat treatment (50–96°C)
increased microparticle average size by up to four-fold to ≈20 μm,
probably due to disulphide cross-linking of kafirin proteins. The
vacuoles within these microparticles enlarged up to >10-fold,
probably due to greater expansion of air within the microparticles
with higher temperature, as the vacuoles are probably footprints of
air bubbles. As with heat treatment, glutaraldehyde (10–30%)
treatment resulted in oval microparticles, up to about four-fold
larger than the control, probably due to covalent
glutaraldehyde-polypeptide linkage. Transglutaminase (0.1–0.6%)
treatment had only slight effect on the size and shape of
microparticles, probably because kafirin has very low lysine
content, inhibiting transglutaminase-catalysed cross-linking
through ε-(-glutamyl)-lysine bonding. Surface morphology using
atomic force microscopy indicated that the microparticles
apparently comprised coalesced nanostructures. With heat and
transglutaminase treatments, the microparticles seemed to be
composed of round nanostructures that coalesced into random
irregular shapes, indicative of non-linear
protein aggregation. In
contrast, with glutaraldehyde treatment, the nanostructures were
spindle-shaped and had a unidirectional orientation, probably due
to linear alignment of the nanostructures controlled by
glutaraldehyde-polypeptide linkage. Thin (<50 μm) films prepared
from kafirin microparticles and conventional cast kafirin films
were compared in terms of their water stability and other related
properties. Films cast from microparticles were more water-stable
compared to conventional kafirin films, probably because the large
vacuoles within the kafirin microparticles may have enhanced
protein solubility in the casting solution, thereby improving the
film matrix cohesion. The films prepared from microparticles
treated with glutaraldehyde were more water-stable compared to the
control, despite the loss of plasticizer, probably due to formation
of the covalent glutaraldehyde-polypeptide linkages. The potential
of modified kafirin microparticles to bind
bone morphogenetic
protein-
2 (BMP-
2) was investigated. Compared to a collagen
standard, the BMP-
2 binding capacity of control, heat-treated,
transglutaminase-treated and glutaraldehyde-treated kafirin
microparticles were 7%, 18%, 34% and 22% higher, respectively,
probably mainly due to the vacuoles within the microparticles
creating greater binding surface area.…
Advisors/Committee Members: Taylor, J.R.N. (John Reginald Nuttall) (advisor), Taylor, Janet (advisor), Naidoo, Vinny (advisor).
Subjects/Keywords: Binding; Bone
morphogenetic protein-2 (bmp-2); Water
stability;
Cross-linking;
Kafirin;
Microparticles;
UCTD
Record Details
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Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Anyango, J. O. (2012). Physico-chemical
modification of kafirin microstructures for application as
biomaterials. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/29708
Chicago Manual of Style (16th Edition):
Anyango, Joseph Ochieng. “Physico-chemical
modification of kafirin microstructures for application as
biomaterials.” 2012. Doctoral Dissertation, University of Pretoria. Accessed March 08, 2021.
http://hdl.handle.net/2263/29708.
MLA Handbook (7th Edition):
Anyango, Joseph Ochieng. “Physico-chemical
modification of kafirin microstructures for application as
biomaterials.” 2012. Web. 08 Mar 2021.
Vancouver:
Anyango JO. Physico-chemical
modification of kafirin microstructures for application as
biomaterials. [Internet] [Doctoral dissertation]. University of Pretoria; 2012. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2263/29708.
Council of Science Editors:
Anyango JO. Physico-chemical
modification of kafirin microstructures for application as
biomaterials. [Doctoral Dissertation]. University of Pretoria; 2012. Available from: http://hdl.handle.net/2263/29708
Texas A&M University
13.
Wang, Shuo.
The Potential Role of Erk1/2 Map Kinases in the Regulation of Neuromuscular Junction Integrity and Bone Morphogenetic Protein Signaling.
Degree: PhD, Biomedical Sciences, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/173504
► It is known that extracellular signal-regulated kinases 1 and 2 (ERK1/2) play important roles in development and maintenance of skeletal muscles in vitro and in…
(more)
▼ It is known that extracellular signal-regulated kinases 1 and
2 (ERK1/
2) play important roles in development and maintenance of skeletal muscles in vitro and in vivo. However, it is not clear whether muscle ERK1/
2 can regulate neuromuscular junction (NMJ) structure and function and (or) have unique substrates and downstream pathways in skeletal muscle. Previously, we generated an ERK 1/
2 double knockout (DKO) mouse line using germ line Erk1 mutation and Cre-loxP deletion of Erk2. Those DKO animals were viable after birth but displayed stunted postnatal growth, muscle weakness, and shorter lifespan. Next, two typical fast-twitch muscles, the sternomastoid (STN) and the tibialis anterior (TA), were examined. A mixture of fiber loss and mild muscle atrophy was found, and STN but not TA underwent partial denervation. To explore the role of ERK1/
2 in slow-twitch, type 1 muscle fibers, we studied the mutant soleus muscle (SOL) in the aspect of morphology, expression of denervation and synaptic markers, and mitochondrial function. Next, we examined the status of
bone morphogenetic (BMP) signaling in mutant SOL and myotubes by measuring mRNA and phosphorylation level of several BMP components.
It is shown that young adult mutant SOL was drastically wasted, with highly atrophied type 1 fibers, denervation at most synaptic sites, induction of “fetal” acetylcholine receptor (AChRγ) subunit, reduction of “adult” AChRε subunit, and impaired mitochondrial function. In weanlings, fiber morphology and mitochondrial markers were normal, yet AChRγ upregulation and AChRε downregulation were
observed. In mutant weanlings, most of the fetal AChRs appeared at NMJs on type1 muscle fibers. These results suggest that: (1) ERK1/
2 are critical for slow-twitch fiber growth; (
2) a defective γ/ε-AChR subunit switch, preferentially at synapses on slow fibers, precedes denervation and wasting of mutant SOL; (3) the neuromuscular synapse is a primary subcellular target for muscle ERK1/
2 function in vivo.
It is shown that mutant SOL exhibited alterations in the BMP pathway: decreased expression of Bmpr1b, unchanged expression of the target gene Id-1, the negative regulator Noggin and the intracellular kinase Smad6, and unchanged phosphorylation level of Smad1/5/8. These experiments suggest that unlike normal muscle, BMP signaling is not activated in mutant SOL after denervation. However, Bmpr1b expression and Smad1/5/8 phosphorylation were not altered in BMP-treated myotubes subjected to pharmacological inhibition of ERK1/
2 activation. These results suggest that ERK1/
2 regulate BMP signaling in SOL through an indirect mechanism.
Advisors/Committee Members: Rimer, Mendell (advisor), Miranda, Rajesh C (committee member), Thompson, Wesley (committee member), Harlow, Mark (committee member).
Subjects/Keywords: Neuromuscular Junction; Acetylcholine Receptor; Extracellular Signal-Regulated Protein Kinase 1 and 2; Bone Morphogenetic Protein
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, S. (2018). The Potential Role of Erk1/2 Map Kinases in the Regulation of Neuromuscular Junction Integrity and Bone Morphogenetic Protein Signaling. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173504
Chicago Manual of Style (16th Edition):
Wang, Shuo. “The Potential Role of Erk1/2 Map Kinases in the Regulation of Neuromuscular Junction Integrity and Bone Morphogenetic Protein Signaling.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 08, 2021.
http://hdl.handle.net/1969.1/173504.
MLA Handbook (7th Edition):
Wang, Shuo. “The Potential Role of Erk1/2 Map Kinases in the Regulation of Neuromuscular Junction Integrity and Bone Morphogenetic Protein Signaling.” 2018. Web. 08 Mar 2021.
Vancouver:
Wang S. The Potential Role of Erk1/2 Map Kinases in the Regulation of Neuromuscular Junction Integrity and Bone Morphogenetic Protein Signaling. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1969.1/173504.
Council of Science Editors:
Wang S. The Potential Role of Erk1/2 Map Kinases in the Regulation of Neuromuscular Junction Integrity and Bone Morphogenetic Protein Signaling. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173504
14.
Vanderman, Kadie S.
EFFECTS OF OSTEOARTHRITIS AND INFLAMMATION ON MENISCUS CELL RESPONSE TO OSTEOGENIC PROTEIN 1 (OP1).
Degree: 2015, Wake Forest University
URL: http://hdl.handle.net/10339/57440
► The long term objective of this project was to determine the response to the Bone Morphogenic Protein (BMP) family member, Osteogenic Protein 1 (OP1/BMP7) in…
(more)
▼ The long term objective of this project was to determine the response to the Bone Morphogenic Protein (BMP) family member, Osteogenic Protein 1 (OP1/BMP7) in meniscus cells. We have focused on the meniscus, because this tissue is highly susceptable to injuries which often fail to heal and lead to osteoarthritis (OA). OA is driven by molecular inflammation which disrupts tissue homeostasis through the upregulation of catabolism (extracellular matrix breakdown) which outpaces anabolism (matrix synthesis). OP1 has been shown to be the most potent natural inducer of articular cartilage matrix synthesis. We chose to evaluate this growth factor for its ability to induce meniscus matrix synthesis during pro-inflammatory factor treatment and OA with the translational objective of assessing OP1 as a potential repair factor after trauma and/or disease of the meniscus. The first aim of this project was to compare the OP1 response of normal and OA menisci. We tested the hypothesis that OP1 stimulation of OA meniscus cells will result in reduced canonical BMP signaling and matrix synthesis compared to normal cells. Our results in normal meniscus cells showed that OP1 stimulated matrix expression and suppressed catabolic factor expression. However, OA meniscus cells were refractory to OP1, and insensitivity to OP1 could be induced in normal cells following treatment with pro-inflammatory factors. Our second aim was to determine the mechanism of OP1 insensitivity during pro-inflammatory cytokine stimulation. As the BMP transcription factor, Smad1, is a major regulatory target of signaling cross talk, we hypothesized that pro-inflammatory cytokine stimulation of normal and osteoarthritis meniscus cells will stimulate inhibitory phosphorylation of the Smad linker region (Ser206). We identified that such cytokine stimulation resulted in significantly increased Smad linker region phosphorylation at Serine 206, part of a MAP kinase phosphorylation consensus site shown to inhibit Smad1 activity. This work is the only identified study of OP1 to assess anabolic insensitivity and target a mechanism contributing to it in meniscal cells. The findings of this work indicate a target to restore anabolic sensitivity to OP1 and aid in the restoration of tissue homeostasis following inflammation.
Subjects/Keywords: bone morphogenetic protein (BMP)
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Vanderman, K. S. (2015). EFFECTS OF OSTEOARTHRITIS AND INFLAMMATION ON MENISCUS CELL RESPONSE TO OSTEOGENIC PROTEIN 1 (OP1). (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/57440
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vanderman, Kadie S. “EFFECTS OF OSTEOARTHRITIS AND INFLAMMATION ON MENISCUS CELL RESPONSE TO OSTEOGENIC PROTEIN 1 (OP1).” 2015. Thesis, Wake Forest University. Accessed March 08, 2021.
http://hdl.handle.net/10339/57440.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vanderman, Kadie S. “EFFECTS OF OSTEOARTHRITIS AND INFLAMMATION ON MENISCUS CELL RESPONSE TO OSTEOGENIC PROTEIN 1 (OP1).” 2015. Web. 08 Mar 2021.
Vancouver:
Vanderman KS. EFFECTS OF OSTEOARTHRITIS AND INFLAMMATION ON MENISCUS CELL RESPONSE TO OSTEOGENIC PROTEIN 1 (OP1). [Internet] [Thesis]. Wake Forest University; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10339/57440.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vanderman KS. EFFECTS OF OSTEOARTHRITIS AND INFLAMMATION ON MENISCUS CELL RESPONSE TO OSTEOGENIC PROTEIN 1 (OP1). [Thesis]. Wake Forest University; 2015. Available from: http://hdl.handle.net/10339/57440
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
15.
Jian, Yongqiang.
The BMPRII Tail Domain Modulates the Magnitude of BMP7 Signalling.
Degree: 2010, University of Toronto
URL: http://hdl.handle.net/1807/25639
► BMPRII, a BMP type II receptor, plays an important role in regulating diverse biological events. BMPRII contains a long carboxyl tail domain, which is highly…
(more)
▼ BMPRII, a BMP type II receptor, plays an important role in regulating diverse biological events. BMPRII contains a long carboxyl tail domain, which is highly conserved among vertebrate species. The tail domain has been shown to regulate cytoskeleton remodeling, whereas the function in regulating canonical BMP signalling is not well studied. Here, I show that the BMPRII tail domain reduces the magnitude of BMP7-induced pSmad1 activation in the early stage, which also changes the magnitude of BMP target gene expression. Furthermore, my data also suggest that the BMPRII tail not only modulates BMP7-induced Smad1 carboxyl terminal phosphorylation, but also inhibits endogenous BMP signalling under overexpression conditions. Finally, BMP7 promotes Neuro2a neurite extension and I demonstrate that knockdown of BMPRII affects BMP7 induced neurite outgrowth. Altogether, these studies demonstrate that the BMPRII tail may play a role in regulating responsiveness to BMP7, and thereby modulates BMP7 dependent neurite extension in neuronal cells.
MAST
Advisors/Committee Members: Attisano, Liliana, Biochemistry.
Subjects/Keywords: BMPRII; Bone morphogenetic protein; 0487
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APA (6th Edition):
Jian, Y. (2010). The BMPRII Tail Domain Modulates the Magnitude of BMP7 Signalling. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25639
Chicago Manual of Style (16th Edition):
Jian, Yongqiang. “The BMPRII Tail Domain Modulates the Magnitude of BMP7 Signalling.” 2010. Masters Thesis, University of Toronto. Accessed March 08, 2021.
http://hdl.handle.net/1807/25639.
MLA Handbook (7th Edition):
Jian, Yongqiang. “The BMPRII Tail Domain Modulates the Magnitude of BMP7 Signalling.” 2010. Web. 08 Mar 2021.
Vancouver:
Jian Y. The BMPRII Tail Domain Modulates the Magnitude of BMP7 Signalling. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1807/25639.
Council of Science Editors:
Jian Y. The BMPRII Tail Domain Modulates the Magnitude of BMP7 Signalling. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25639
Loma Linda University
16.
Yalung, Jelson.
Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model.
Degree: MS, Orthodontics and Dentofacial Orthopedics, 2012, Loma Linda University
URL: https://scholarsrepository.llu.edu/etd/107
► Introduction: The utilization of recombinant human Bone Morphogenetic Protein-2 (rh- BMP2) to form new bone has been shown to be a promising alternative to autogenous…
(more)
▼ Introduction: The utilization of recombinant human
Bone Morphogenetic Protein-
2 (rh- BMP2) to form new
bone has been shown to be a promising alternative to autogenous
bone grafts. Understanding the biomechanical properties of rhBMP-
2 restored mandibular defects would provide useful knowledge in the future success of orthopedic and dental treatment in patients who have had restoration of mandibular defects with rhBMP-
2. The aim of the study was to evaluate and compare the biomechanical characteristics of rhBMP-
2 regenerated
bone in mandibular defects using
2 different concentrations of rhBMP-
2 with a given carrier in non-human primates Material and Methods: Critical-sized defects (approximately
2.5 cm) were created in the mandibles of 6 adult male non-human primates. Each side of the mandibles received one of
2 carrier types: 1) 1.35 mg/mL rhBMP-
2 combined with a collagen ceramic sponge (CCS) and
2) 0.75 mg/mL rhBMP-
2 combined with CCS. All defects were stabilized with a titanium reconstruction plate. Young's modulus of 10
bone samples was calculated using the results from a tensile test of the samples. Density of the 10 samples was determined with a pycnometer. A
2-dimensional model of the mandible was virtually created to simulate the mandible for a given BMP/carrier group. Subdomains of the model included cortical
bone, regenerated
bone, periodontal ligament, enamel, and cementum. Boundary conditions of the subdomains were assigned using the biomechanical properties determined in the literature and the regenerated
bone values were assigned based on prior testing. Finite Element Analysis was performed with the COMSOL Finite Element Modeling software to measure peak Von Mises stresses and surface displacement of the newly regenerated
bone in response to a 150 N force of the masseter muscle. Results: There was no statistical difference between the mechanical properties among treatment groups. However, both treatment groups showed a statistically significant difference in stress distribution, displacement in the x-axis, and displacement in the yaxis when compared to the control group (p < 0.05) but no statistically significant difference when compared to each other (p < 0.05). Conclusions: The differences in stress distribution and displacement when comparing the treatment group to the control group indicate that the treatment group regenerated
bone was less stiff which lead to more displacement in the mandible and higher stress in response to function. This may be attributed to the 6-month follow-up period where the regenerated
bone did not complete mineralization. The similarity in mechanical properties, stress, and displacement between treatment groups indicate that a rhBMP-
2 concentration of 0.75 mg/mL produced
bone that was biomechanically comparable to a concentration of 1.35 mg/mL.
Advisors/Committee Members: Caruso, Joseph M., Herford, Alan S., Rungcharassaeng, Kitichai.
Subjects/Keywords: Orthodontics and Orthodontology; Other Dentistry; Bone Morphogenetic Proteins; Bone Development; Growth Substances; Recombinant Proteins; Bone Morphogenetic Protein 2; Bone regeneration; Mandible - surgery; Mandibular defects; Recombinant human bone morphogenetic protein-2; bone growth
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APA (6th Edition):
Yalung, J. (2012). Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model. (Thesis). Loma Linda University. Retrieved from https://scholarsrepository.llu.edu/etd/107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yalung, Jelson. “Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model.” 2012. Thesis, Loma Linda University. Accessed March 08, 2021.
https://scholarsrepository.llu.edu/etd/107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yalung, Jelson. “Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model.” 2012. Web. 08 Mar 2021.
Vancouver:
Yalung J. Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model. [Internet] [Thesis]. Loma Linda University; 2012. [cited 2021 Mar 08].
Available from: https://scholarsrepository.llu.edu/etd/107.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yalung J. Mechanical Evaluation of Mandibular Defects Restored with rhBMP-2: A Finite Element Model. [Thesis]. Loma Linda University; 2012. Available from: https://scholarsrepository.llu.edu/etd/107
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
17.
Chung, Yueh-hua.
The role and effect of bone morphogenetic protein-2 in liver fibrosis.
Degree: Master, Biological Sciences, 2007, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827107-112806
► Bone Morphogenetic proteins (BMPs) belong to transforming growth factor beta (TGF-β) superfamily. They regulate cell proliferation, cell differentiation, and bone morphogenesis. Previous evidence suggests that…
(more)
▼ Bone Morphogenetic proteins (BMPs) belong to transforming growth factor beta (TGF-β) superfamily. They regulate cell proliferation, cell differentiation, and
bone morphogenesis. Previous evidence suggests that BMP-
2, as an antagonist of TGF-β, may play an inhibitory role in tissue fibrogenesis. The aim of this study is to examine the expression profile of BMP-
2 in fibrotic livers and to test whether BMP-
2 gene delivery could alleviate or reverse the liver fibrogenesis models in mice including bile duct ligation (BDL) or carbon tetrachloride (CCl4) model. The results showed that the AST, ALT, and bilirubin levels in sera and the expression of TGF-β, α-smooth muscle actin, type I collagen in livers were significantly up-regulated by BDL surgery or CCl4 administration. After BDL, the hepatic BMP-
2 mRNA and
protein levels in mice decreased at 7 and 14 days after surgery. Similarly, the hepatic BMP-
2 mRNA and
protein levels in mice decreased at day 14 and 28 after CCl4 administration. BMP-
2 gene delivery alleviated the inflammation and the liver injury caused by BDL or CCl4 exposure. These findings strongly suggest that BMP-
2 is involved in the pathogenesis of liver fibrosis. Moreover, BMP-
2 supplementation may facilitate a novel strategy for treatment of liver fibrosis.
Advisors/Committee Members: Cho Chung-Lung (chair), Tai Ming-Hong (committee member), Hu Tsung-hui (chair).
Subjects/Keywords: liver fibrosis; Bone morphogenetic protein-2
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APA (6th Edition):
Chung, Y. (2007). The role and effect of bone morphogenetic protein-2 in liver fibrosis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827107-112806
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chung, Yueh-hua. “The role and effect of bone morphogenetic protein-2 in liver fibrosis.” 2007. Thesis, NSYSU. Accessed March 08, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827107-112806.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chung, Yueh-hua. “The role and effect of bone morphogenetic protein-2 in liver fibrosis.” 2007. Web. 08 Mar 2021.
Vancouver:
Chung Y. The role and effect of bone morphogenetic protein-2 in liver fibrosis. [Internet] [Thesis]. NSYSU; 2007. [cited 2021 Mar 08].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827107-112806.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chung Y. The role and effect of bone morphogenetic protein-2 in liver fibrosis. [Thesis]. NSYSU; 2007. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0827107-112806
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Temple University
18.
Mundy, Christina Maria.
The Interaction Between Connective Tissue Growth Factor and Bone Morphogenetic Protein-2 During Osteoblast Differentiation and Function.
Degree: PhD, 2014, Temple University
URL: http://digital.library.temple.edu/u?/p245801coll10,269581
► Cell Biology
Connective tissue growth factor (CTGF/CCN2) and bone morphogenetic protein (BMP)-2 are both produced and secreted by osteoblasts. Both proteins have been shown to…
(more)
▼ Cell Biology
Connective tissue growth factor (CTGF/CCN2) and bone morphogenetic protein (BMP)-2 are both produced and secreted by osteoblasts. Both proteins have been shown to have independent effects in regulating osteoblast proliferation, maturation and mineralization. However, how these two proteins interact during osteoblast differentiation remains unknown. In Chapters 2 and 3, we utilized two cell culture model systems, osteoblasts derived from CTGF knockout (KO) mice and osteoblasts infected with an adenovirus, which over-expresses CTGF (Ad-CTGF), to investigate the effects of CTGF and BMP-2 on osteoblast development and function in vitro. To observe differences in osteoblast maturation and mineralization, we performed alkaline phosphatase (ALP) staining and activity and alizarin red staining, respectively. Contrary to a previously published report, osteoblast maturation and mineralization were similar in osteogenic cultures derived from KO and wild type (WT) calvaria in the absence of BMP-2 stimulation. Interestingly, in KO and WT osteoblast cultures stimulated with BMP-2, the KO osteoblast cultures exhibited increased alkaline phosphatase staining and activity and had larger, fused nodules stained with alizarin red than WT osteoblast cultures. This increase in osteoblast differentiation was accompanied by increased protein levels of phosphorylated Smad 1/5/8 and mRNA expression levels of bone morphogenetic protein receptor Ib. These data confirm enhanced osteoblast maturation and mineralization in BMP-2 induced KO osteoblast cultures. We also examined osteoblast differentiation in cultures that were infected with Ad-CTGF and in control cultures. Continuous over-expression of CTGF resulted in decreased ALP staining and activity, alizarin red staining, and mRNA expression of osteoblast markers in both unstimulated and BMP-2 stimulated cultures. Impaired osteoblast differentiation in cultures over-expressing CTGF was accompanied by decreased protein levels of phosphorylated Smad 1/5/8. In addition to the functional assays that we performed on WT and KO osteoblast cultures, we performed ChIP assays to investigate differences in binding occupancy of transcription factors on the Runx2 and Osteocalcin promoters in BMP-2 induced WT and KO osteoblast cultures. We demonstrate that in BMP-2 induced WT and KO osteoblast cultures, there was greater Smad 1 and JunB occupancy on the Runx2 promoter and Runx2 occupancy on the Osteocalcin promoter in BMP-2 induced KO osteoblast cultures compared to WT cultures. Collectively, the data demonstrate that CTGF acts to negatively regulate BMP-2 induced signaling and osteoblast differentiation. In Chapter 4, we synthesized an active His-tagged BMP-2 recombinant protein to track surface binding of BMP-2 in CTGF WT and KO osteoblasts. We amplified mature BMP-2 in genomic DNA, which was inserted correctly into a pET-28b(+) vector. We ran a SDS-PAGE gel and stained with Coomassie blue to show that we successfully induced BMP-2 in bacteria cells, extracted the protein using urea, and…
Advisors/Committee Members: Popoff, Steven N.;, Abood, Mary Ellen, Denny, Michael F., Goldfinger, Lawrence, Litvin, Judith, Monroy, Maria A.;.
Subjects/Keywords: Cellular biology;
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APA ·
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APA (6th Edition):
Mundy, C. M. (2014). The Interaction Between Connective Tissue Growth Factor and Bone Morphogenetic Protein-2 During Osteoblast Differentiation and Function. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,269581
Chicago Manual of Style (16th Edition):
Mundy, Christina Maria. “The Interaction Between Connective Tissue Growth Factor and Bone Morphogenetic Protein-2 During Osteoblast Differentiation and Function.” 2014. Doctoral Dissertation, Temple University. Accessed March 08, 2021.
http://digital.library.temple.edu/u?/p245801coll10,269581.
MLA Handbook (7th Edition):
Mundy, Christina Maria. “The Interaction Between Connective Tissue Growth Factor and Bone Morphogenetic Protein-2 During Osteoblast Differentiation and Function.” 2014. Web. 08 Mar 2021.
Vancouver:
Mundy CM. The Interaction Between Connective Tissue Growth Factor and Bone Morphogenetic Protein-2 During Osteoblast Differentiation and Function. [Internet] [Doctoral dissertation]. Temple University; 2014. [cited 2021 Mar 08].
Available from: http://digital.library.temple.edu/u?/p245801coll10,269581.
Council of Science Editors:
Mundy CM. The Interaction Between Connective Tissue Growth Factor and Bone Morphogenetic Protein-2 During Osteoblast Differentiation and Function. [Doctoral Dissertation]. Temple University; 2014. Available from: http://digital.library.temple.edu/u?/p245801coll10,269581
19.
中野, 嗣久.
Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells : ヒト歯根膜細胞におけるMTAの骨形成タンパク質発見と石灰化の誘導について.
Degree: 博士（歯学）, 2013, Kyushu University / 九州大学
URL: http://hdl.handle.net/2324/18926
;
http://dx.doi.org/10.15017/18926
► Introduction: Mineral trioxide aggregate (MTA) is a therapeutic, endodontic repair material that is reported to exhibit calcified tissue-conductive activity although the mechanisms remain unclear. We…
(more)
▼ Introduction: Mineral trioxide aggregate (MTA) is a therapeutic, endodontic repair material that is reported to exhibit calcified tissue-conductive activity although the mechanisms remain unclear. We hypothesize that the dissolution of calcium from MTA into the surrounding environment may play an important role in the osteoblastic/ cementoblastic differentiation of human periodontal ligament cells (HPLCs). Methods: Two populations of HPLCs were obtained from two patients, respectively, and were cultured in the presence or absence of MTA discs and/or CaCl2 in order to investigate calcium release, calcification activity, calcium-sensing receptor (CaSR) gene expression and
bone morphogenetic protein-
2 (BMP-
2), and BMP-
2 receptor
protein and gene expression. Results: MTA released a substantial accumulation of calcium (4 mmol/L) within 14 days into culture media. After 4 weeks, the two populations of HPLCs independently exhibited calcification as well as BMP-
2 distribution in the vicinity of MTA. HPLCs inherently expressed genes encoding for the CaSR and BMP-
2 receptors. Exogenous CaCl2 media supplementation induced CaSR gene expression in HPLCs and calcification and BMP-
2 synthesis throughout the entire HPLC cultures, whereas MgCl2 had no effect. Both MTA and CaCl2 stimulated BMP-
2 gene expression above that of baseline levels. Conclusion: Here we show the first report showing that HPLCs cocultured directly with MTA upregulated BMP2 expression and calcification. These results may be through CaSR interactions that were potentially activated by the release of calcium from MTA into the culture environment.
Advisors/Committee Members: 久木田, 敏夫, 前田, 英史.
Subjects/Keywords: Bone morphogenetic protein 2; calcification; calcium; mineral trioxide aggregate; periodontal ligament cells
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APA (6th Edition):
中野, . (2013). Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells : ヒト歯根膜細胞におけるMTAの骨形成タンパク質発見と石灰化の誘導について. (Thesis). Kyushu University / 九州大学. Retrieved from http://hdl.handle.net/2324/18926 ; http://dx.doi.org/10.15017/18926
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
中野, 嗣久. “Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells : ヒト歯根膜細胞におけるMTAの骨形成タンパク質発見と石灰化の誘導について.” 2013. Thesis, Kyushu University / 九州大学. Accessed March 08, 2021.
http://hdl.handle.net/2324/18926 ; http://dx.doi.org/10.15017/18926.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
中野, 嗣久. “Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells : ヒト歯根膜細胞におけるMTAの骨形成タンパク質発見と石灰化の誘導について.” 2013. Web. 08 Mar 2021.
Vancouver:
中野 . Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells : ヒト歯根膜細胞におけるMTAの骨形成タンパク質発見と石灰化の誘導について. [Internet] [Thesis]. Kyushu University / 九州大学; 2013. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2324/18926 ; http://dx.doi.org/10.15017/18926.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
中野 . Mineral Trioxide Aggregate Induces Bone Morphogenetic Protein-2 Expression and Calcification in Human Periodontal Ligament Cells : ヒト歯根膜細胞におけるMTAの骨形成タンパク質発見と石灰化の誘導について. [Thesis]. Kyushu University / 九州大学; 2013. Available from: http://hdl.handle.net/2324/18926 ; http://dx.doi.org/10.15017/18926
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Patel, Janki Jayesh.
Single and Dual Growth Factor Delivery from Poly-E-caprolactone Scaffolds for Pre-Fabricated Bone Flap Engineering.
Degree: PhD, Biomedical Engineering, 2015, University of Michigan
URL: http://hdl.handle.net/2027.42/111451
► Autografts are utilized to reconstruct large craniofacial bone defects; however, they result in donor site morbidity and defect geometry mismatch. Pre-fabricating a bone flap overcomes…
(more)
▼ Autografts are utilized to reconstruct large craniofacial
bone defects; however, they result in donor site morbidity and defect geometry mismatch. Pre-fabricating a
bone flap overcomes these drawbacks by integrating a patient specific scaffold with biologics, implanting it in the latissimus dorsi for a period of time and then transplanting it to the defect site as a partially remodeled construct. Polycaprolactone (PCL) is a biocompatible polymer that has mechanical properties suitable for
bone tissue engineering. It must be integrated with biologics, however, to stimulate
bone formation. The purpose of this work was to investigate
bone regeneration using PCL and dual
protein delivery.
Bone morphogenetic protein-
2 (BMP2) was adsorbed or conjugated onto a PCL scaffold in a clinically applicable setting (1 hour exposure at room temperature). Adsorbed BMP2 had a small burst release and was bioactive as indicated by C2C12 alkaline phosphatase expression. Interestingly, conjugated BMP2 had a sustained release but was not bioactive. When implanted subcutaneously, adsorbed BMP2 had increased
bone volume (BV), elastic modulus, and ingrowth when compared to conjugation. Next, a collagen sponge was fabricated inside of a BMP2-adsorbed PCL scaffold to deliver vascular endothelial growth factor (VEGF). In addition, a modular PCL scaffold was developed in which the inner and outer modular portions were adsorbed with BMP2 and VEGF, respectively. In both systems, the VEGF was bioactive as indicated by increased endothelial cell proliferation. Dual delivery of BMP2+VEGF significantly increased BV from 4 to 8 weeks in an ectopic location, whereas, BMP2 alone did not. Finally, erythropoietin (EPO) and BMP2 were delivered from the outer and inner portions of the modular scaffold, respectively. The adsorbed EPO was bioactive as indicated by increased endothelial cell proliferation. At 4 weeks, dual EPO+BMP2 delivery significantly increased BV and ingrowth when compared to BMP2 alone. In conclusion, adsorbing BMP2 onto PCL may be optimal for clinical use. Delivering VEGF with BMP2 increases the
bone regeneration rate from 4 to 8 weeks, and delivering EPO with BMP2 increases the BV at 4 weeks when compared to BMP2 alone, making multiple biologics delivery a promising method to increase the regenerated
bone for pre-fabricated flaps.
Advisors/Committee Members: Hollister, Scott J. (committee member), Krebsbach, Paul (committee member), Stegemann, Jan Philip (committee member), Edwards, Sean Peter (committee member).
Subjects/Keywords: Bone Tissue Engineering; Polycaprolactone; Bone Morphogenetic Protein-2; Vascular Endothelial Growth Factor; Erythropoietin; Biomedical Engineering; Engineering; Science
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APA (6th Edition):
Patel, J. J. (2015). Single and Dual Growth Factor Delivery from Poly-E-caprolactone Scaffolds for Pre-Fabricated Bone Flap Engineering. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/111451
Chicago Manual of Style (16th Edition):
Patel, Janki Jayesh. “Single and Dual Growth Factor Delivery from Poly-E-caprolactone Scaffolds for Pre-Fabricated Bone Flap Engineering.” 2015. Doctoral Dissertation, University of Michigan. Accessed March 08, 2021.
http://hdl.handle.net/2027.42/111451.
MLA Handbook (7th Edition):
Patel, Janki Jayesh. “Single and Dual Growth Factor Delivery from Poly-E-caprolactone Scaffolds for Pre-Fabricated Bone Flap Engineering.” 2015. Web. 08 Mar 2021.
Vancouver:
Patel JJ. Single and Dual Growth Factor Delivery from Poly-E-caprolactone Scaffolds for Pre-Fabricated Bone Flap Engineering. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2027.42/111451.
Council of Science Editors:
Patel JJ. Single and Dual Growth Factor Delivery from Poly-E-caprolactone Scaffolds for Pre-Fabricated Bone Flap Engineering. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/111451
Boston University
21.
Bui, Matthew.
The effect of phosphate deficiency on BMP-2 treated C3H10T1/2 mesenchymal stem cells.
Degree: MS, Medical Sciences, 2018, Boston University
URL: http://hdl.handle.net/2144/30902
► There are approximately 600,000 cases of delayed or aberrant fracture healing in people each year, with a small subset of these fractures experiencing disunion. Dietary…
(more)
▼ There are approximately 600,000 cases of delayed or aberrant fracture healing in people each year, with a small subset of these fractures experiencing disunion. Dietary phosphate deficiency has been shown to impair oxidative phosphorylation and decrease BMP-
2 mediated chondrogenic differentiation during fracture healing. Prior studies using pre-committed chondro-progenitor ATDC5 cell line grown in phosphate deficient media showed that energy consumption was linked to
protein production and collagen hydroxylation but inversely related to matrix mineralization. The goal of this study was to further define the relationship between energy consumption and BMP-
2 mediated stem cell chondrogenic differentiation and further examine how dietary phosphate, and promotion of collagen hydroxylation via ascorbate availability effected these processes.
C3H10T1/
2 murine cells, a multi-potential cell line, were expanded in pre-differentiation growth medium (DMEM with 10% FBS and 1% Pen/Strep). Once cells reached 60% confluence (day 0), they were grown in differentiating media (α-MEM with 5% FBS and 1X insulin-transferrin-selenium) containing either 100% (1mM) or 25% (0.25mM) inorganic phosphate (Pi), ± 200ng/mL BMP-
2(BMP), and ±0.
2 mM L-ascorbic acid (AA). In total, there were 8 groups with varying combinations of these three substances. Intracellular lipid, total DNA,
protein, and hydroxyproline (HP) content were examined. Chondrocyte gene expression (Col2a1, Acan, ColXa1) and adipocyte gene expression (Pparg, Plin1, Ucp1) were measured to check for cell lineage commitment and specific differentiation of the C3H10T1/
2. All measurements were acquired at day 8.
The +BMP differentiation media groups contained significantly less DNA content and more
protein content than the –BMP differentiation media groups (both p<0.0001). There was also a significant interaction between phosphate and ascorbic acid treatment (p=0.0296), with 25% Pi +AA groups producing significantly more
protein than 100% Pi +AA groups. Hydroxyproline production was not different in 100% Pi or 25% Pi conditions (p=0.2951). AA presence in culture media led to greater HP production than culture media lacking AA (p=0.0035) There was a trend of an interaction between phosphate content and AA availability (p=0.0744). 100% Pi ±AA groups produced significantly different amounts of HP while 25% Pi ±AA groups did not produce significantly different amount of HP. Col2a1, Acan, and ColXa1 expression were all increased in +BMP groups. Ascorbic acid treatment groups expressed significantly more Col2a1and Acan than –AA groups. 100% Pi media led to greater Acan expression over 25% Pi groups (p=0.0009), whereas 25% Pi media trended to lead to greater ColXa1 expression over 100% Pi groups (p=0.0734). Pparg and Plin1 expression were increased in the 25% Pi condition. There were no significant differences in expression of Ucp1.
C3H10T1/
2 cells were significantly affected by phosphate concentration, BMP-
2 treatment, and ascorbic acid supplementation. Phosphate deficiency hindered…
Advisors/Committee Members: Gerstendfeld, Louis C. (advisor), Hussein, Amira I. (advisor).
Subjects/Keywords: Medicine; Bone development; Bone morphogenetic protein 2; Cell differentiation; Cell lineage commitment; Fracture healing; Mesenchymal stem cell
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APA (6th Edition):
Bui, M. (2018). The effect of phosphate deficiency on BMP-2 treated C3H10T1/2 mesenchymal stem cells. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/30902
Chicago Manual of Style (16th Edition):
Bui, Matthew. “The effect of phosphate deficiency on BMP-2 treated C3H10T1/2 mesenchymal stem cells.” 2018. Masters Thesis, Boston University. Accessed March 08, 2021.
http://hdl.handle.net/2144/30902.
MLA Handbook (7th Edition):
Bui, Matthew. “The effect of phosphate deficiency on BMP-2 treated C3H10T1/2 mesenchymal stem cells.” 2018. Web. 08 Mar 2021.
Vancouver:
Bui M. The effect of phosphate deficiency on BMP-2 treated C3H10T1/2 mesenchymal stem cells. [Internet] [Masters thesis]. Boston University; 2018. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2144/30902.
Council of Science Editors:
Bui M. The effect of phosphate deficiency on BMP-2 treated C3H10T1/2 mesenchymal stem cells. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/30902
Freie Universität Berlin
22.
Huber, Elisabeth.
Effect of demineralized bone matrix (DBM) and enrichments with BMP-2 or
gentamicin on bone healing in a sheep model.
Degree: 2016, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-8912
► A consequence of critical-size bone defects, mechanical instability or infection of fractures can be an impaired bone healing. To treat or prevent this consequence a…
(more)
▼ A consequence of critical-size
bone defects, mechanical instability or
infection of fractures can be an impaired
bone healing. To treat or prevent
this consequence a wide spectre of
bone grafting materials are used.
Autologous
bone graft is considered the gold standard in
bone grafting
procedures but due to its disadvantages, there is a need for an alternative.
This study evaluated the intraoperative application of demineralized
bone
matrix (DBM) and the enrichment with BMP-
2 and Gentamicin as an alternative to
autologous spongiosa in a drill hole model in sheep. Drill hole defects
(diameter 6mm x depth max. 15 mm) were placed in the proximal and distal part
of femur, humerus, metakarpus and metatarsus of merino-mix sheep and filled
with either DBM, DBM enriched with BMP-
2 or DBM enriched with Gentamicin.
Defects filled with autologous spongiosa and empty defects served as a
control. After three and nine weeks healing time the samples were analysed
radiographically, histologically and histochemically. The singular use of DBM
showed a similar
bone healing as the empty controls and no enhancement of the
bone healing compared to the group treated with autologous spongiosa. DBM
enriched with BMP-
2 enhanced
bone healing to a similar extent as the group
treated with autologous spongiosa. Enriched with Gentamicin the
bone healing
was reduced. Based on the long-term binding of BMP-
2 in DBM combined with a
continuous release, DBM is a good carrier for BMP-
2. This is therefore a
suitable combination to treat an impaired
bone healing or critical size
defects as an alternative to autologous spongiosa. The combination with
Gentamicin delayed the
bone healing but did not inhibit it. This combination
maybe holds advantages in case of an infected fracture or osteomyelitis, but
this still has to be determined by further studies. DBM alone cannot stand as
an alternative to autologous spongiosa in terms of enhancing the
bone healing,
but it holds a promising potency as a carrier and therefore for the treatment
of critical size defects or infected fractures.
Advisors/Committee Members: w (gender), Prof. Dr. Robert Klopfleisch (firstReferee), Prof. Dr. Britt Wildemann (furtherReferee), Univ.-Prof. Dr. Leo Brunnberg (furtherReferee).
Subjects/Keywords: sheep; animal models; bone fractures; fracture fixation; gentamicin; bone morphogenetic protein 2 (MeSH); histology; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
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APA (6th Edition):
Huber, E. (2016). Effect of demineralized bone matrix (DBM) and enrichments with BMP-2 or
gentamicin on bone healing in a sheep model. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8912
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Huber, Elisabeth. “Effect of demineralized bone matrix (DBM) and enrichments with BMP-2 or
gentamicin on bone healing in a sheep model.” 2016. Thesis, Freie Universität Berlin. Accessed March 08, 2021.
http://dx.doi.org/10.17169/refubium-8912.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Huber, Elisabeth. “Effect of demineralized bone matrix (DBM) and enrichments with BMP-2 or
gentamicin on bone healing in a sheep model.” 2016. Web. 08 Mar 2021.
Vancouver:
Huber E. Effect of demineralized bone matrix (DBM) and enrichments with BMP-2 or
gentamicin on bone healing in a sheep model. [Internet] [Thesis]. Freie Universität Berlin; 2016. [cited 2021 Mar 08].
Available from: http://dx.doi.org/10.17169/refubium-8912.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Huber E. Effect of demineralized bone matrix (DBM) and enrichments with BMP-2 or
gentamicin on bone healing in a sheep model. [Thesis]. Freie Universität Berlin; 2016. Available from: http://dx.doi.org/10.17169/refubium-8912
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Polo, Cristiane Ibanhes.
A utilização da proteína morgogenética óssea recombinante humana 2 com carreadores adicionais: análise histomorfométrica e por microtomografia computadorizada 3D.
Degree: PhD, Cirurgia e Traumatologia Buco-Maxilo-Faciais, 2013, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/23/23149/tde-28082014-153908/
;
► A utilização de osteoindutores como a proteína morfogenética óssea recombinante humana tipo 2 (rhBMP-2) em cirurgia oral e maxilofacial para reparação e regeneração óssea tem…
(more)
▼ A utilização de osteoindutores como a proteína morfogenética óssea recombinante humana tipo 2 (rhBMP-2) em cirurgia oral e maxilofacial para reparação e regeneração óssea tem aumentado progressivamente. Porém, suas indicações ainda estão limitadas a preenchimento de cavidades e pequenas reconstruções. Este estudo teve como objetivo analisar e comparar, por meio da microtomografia computadorizada tridimensional (Micro-TC 3D) e histomorfometria, a arquitetura óssea, a taxa de osso neoformado e a taxa de biodegradação do -Tricálcio Fostato (-TCP), Fosfato de Cálcio Bifásico (BCP) e Osso Mineral Bovino (BBM), utilizados como carreadores adicionais à rhBMP-2/esponja de colágeno (ACS) em um modelo de regeneração óssea guiada (ROG) vertical em calvária de coelhos. Quatro cilindros de titânio foram fixados à calvária de 20 coelhos da raça Nova Zelândia. No Grupo 1 (n = 10), 3 cilindros foram aleatoriamente preenchidos com um dos materiais teste utilizados como carreadores e um cilindro foi preenchido com coágulo sanguíneo (CO). No Grupo 2 (n = 10), os cilindros foram aleatoriamente designados para os mesmos materiais e coágulo sanguíneo, com a adição da rhBMP-2/ACS. Após 14 semanas de reparação, as amostras foram coletadas e enviadas para a aquisição de imagens da Micro-TC e processamento histológico. De acordo com a análise histomorfométrica, a área óssea média para o Grupo 2 (com rhBMP-2) foi maior do que no Grupo 1 (sem rhBMP-2) para os materiais BCP e -TCP (p <0,001). Não houve diferença entre os grupos para BBM e CO (p> 0,05). Em relação às taxas de reabsorção, a área média dos materiais remanescentes no Grupo 2 foi menor do que no Grupo 1 para todos os materiais (p <0,001) e BBM e -TCP obtiveram a maior taxa de reabsorção nos dois grupos (BBM = -TCP > BCP). A análise da micro-TC revelou que no Grupo 2, BCP e -TCP apresentaram maior volume ósseo médio (BV) do que no Grupo 1 (p <0,05). Não houve diferença entre os grupos para os materiais BBM e CO (p> 0,05). O volume médio de materiais restantes (MV) para o Grupo 2 foi menor do que no Grupo 1 para BBM e -TCP (p <0,05), sem diferença significante entre os grupos para BCP (p = 0,848). A análise dos parâmetros Fator Padrão Trabecular (Tb.Pf) e Índice de Modelo Estrutural (SMI) mostrou no Grupo 2 valores negativos para BCP e -TCP, indicando melhor interconectividade e presença de arquitetura trabecular mais achatada e côncava, indicadores de melhor qualidade e resistência óssea. Pelos resultados apresentados concluiu-se que a utilização da rhBMP-2/ACS associada aos materiais carreadores BCP (Fosfato de Cálcio Bifásico) e -TCP (-Fosfato Tricálcio) aumentou significativamente a formação óssea neste modelo de ROG em calvária de coelhos além de acelerar a biodegradação dos materiais BBM (Osso Mineral Bovino) e -TCP (-Fosfato Tricálcio) neste modelo de ROG.
The use of osteoinductors as the human recombinant bone morphogenetic protein 2 type 2 (rhBMP-2) in oral and maxillofacial surgery for bone regeneration and repair has progressively increased. However, indications are still…
Advisors/Committee Members: Homem, Maria da Graça Naclerio.
Subjects/Keywords: Bone morphogenetic protein 2; Bone regeneration; Bone substitutes; Engenharia de tecidos; Materiais biocompatíveis; Osteogênese; Osteogenesis; Proteína morfogenética do osso 2; Regeneração óssea; Substitutos ósseos; Tissue engineering
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Polo, C. I. (2013). A utilização da proteína morgogenética óssea recombinante humana 2 com carreadores adicionais: análise histomorfométrica e por microtomografia computadorizada 3D. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/23/23149/tde-28082014-153908/ ;
Chicago Manual of Style (16th Edition):
Polo, Cristiane Ibanhes. “A utilização da proteína morgogenética óssea recombinante humana 2 com carreadores adicionais: análise histomorfométrica e por microtomografia computadorizada 3D.” 2013. Doctoral Dissertation, University of São Paulo. Accessed March 08, 2021.
http://www.teses.usp.br/teses/disponiveis/23/23149/tde-28082014-153908/ ;.
MLA Handbook (7th Edition):
Polo, Cristiane Ibanhes. “A utilização da proteína morgogenética óssea recombinante humana 2 com carreadores adicionais: análise histomorfométrica e por microtomografia computadorizada 3D.” 2013. Web. 08 Mar 2021.
Vancouver:
Polo CI. A utilização da proteína morgogenética óssea recombinante humana 2 com carreadores adicionais: análise histomorfométrica e por microtomografia computadorizada 3D. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Mar 08].
Available from: http://www.teses.usp.br/teses/disponiveis/23/23149/tde-28082014-153908/ ;.
Council of Science Editors:
Polo CI. A utilização da proteína morgogenética óssea recombinante humana 2 com carreadores adicionais: análise histomorfométrica e por microtomografia computadorizada 3D. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/23/23149/tde-28082014-153908/ ;
24.
結城, 宗浩.
Bone morphogenetic protein受容体の組織特異的役割 : Tissue-specific function of bone morphogenetic protein receptor in mice; Bone morphogenetic protein ジュヨウタイ ノ ソシキ トクイテキ ヤクワリ.
Degree: Nara Institute of Science and Technology / 奈良先端科学技術大学院大学
URL: http://hdl.handle.net/10061/4159
Subjects/Keywords: Bone morphogenetic protein
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APA ·
Chicago ·
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Manager
APA (6th Edition):
結城, . (n.d.). Bone morphogenetic protein受容体の組織特異的役割 : Tissue-specific function of bone morphogenetic protein receptor in mice; Bone morphogenetic protein ジュヨウタイ ノ ソシキ トクイテキ ヤクワリ. (Thesis). Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10061/4159
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
結城, 宗浩. “Bone morphogenetic protein受容体の組織特異的役割 : Tissue-specific function of bone morphogenetic protein receptor in mice; Bone morphogenetic protein ジュヨウタイ ノ ソシキ トクイテキ ヤクワリ.” Thesis, Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Accessed March 08, 2021.
http://hdl.handle.net/10061/4159.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
結城, 宗浩. “Bone morphogenetic protein受容体の組織特異的役割 : Tissue-specific function of bone morphogenetic protein receptor in mice; Bone morphogenetic protein ジュヨウタイ ノ ソシキ トクイテキ ヤクワリ.” Web. 08 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
結城 . Bone morphogenetic protein受容体の組織特異的役割 : Tissue-specific function of bone morphogenetic protein receptor in mice; Bone morphogenetic protein ジュヨウタイ ノ ソシキ トクイテキ ヤクワリ. [Internet] [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10061/4159.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Council of Science Editors:
結城 . Bone morphogenetic protein受容体の組織特異的役割 : Tissue-specific function of bone morphogenetic protein receptor in mice; Bone morphogenetic protein ジュヨウタイ ノ ソシキ トクイテキ ヤクワリ. [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; Available from: http://hdl.handle.net/10061/4159
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Royal Holloway, University of London
25.
Nkola Tatsinkam, Arnold Junior.
The heparin binding properties of the bone morphogenetic protein antagonist gremlin.
Degree: PhD, 2015, Royal Holloway, University of London
URL: https://pure.royalholloway.ac.uk/portal/en/publications/the-heparin-binding-properties-of-the-bone-morphogenetic-protein-antagonist-gremlin(ccdab987-aacd-4fad-8a1e-cb952dcd03fa).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792292
► Gremlin, a 184 amino acid glycosylated protein, is one of several high-affinity endogenous antagonists of the bone morphogenetic protein (BMP) cytokines including BMPs -2, -4…
(more)
▼ Gremlin, a 184 amino acid glycosylated protein, is one of several high-affinity endogenous antagonists of the bone morphogenetic protein (BMP) cytokines including BMPs -2, -4 and -7. Both gremlin and its BMP ligands have been shown to bind tightly to heparan sulfate (HS) polysaccharides present on cell surfaces. However, the role played by HS in the interaction between gremlin and its BMP ligands remains unknown. In this study, the heparin binding site on gremlin was characterised. Since the three-dimensional structure of gremlin remains experimentally unresolved, prediction of its heparin binding site was based on docking calculations using its homology models. Within the primary sequence, the predicted heparin binding site comprises interspersed basic residue clusters of arginines and lysines. Using site-directed mutagenesis, strategic combinatorial substitutions of these basic clusters were made. Six C-Myc-tagged gremlin mutants, MGRs 1 - 6, were cloned and expressed in parallel with a C-Myctagged wildtype gremlin protein. All six gremlin-Myc mutants showed markedly reduced heparin binding compared to the wildtype, using heparin affinity chromatography and a heparin-binding ELISA. This verified the predicted mapping of the heparin/HS binding site on gremlin. Ion-exchange chromatography of these gremlinMyc proteins on sulfopropyl (SP)-Sepharose columns showed comparatively weak binding, thus indicating that the interaction between gremlin and heparin/HS is specific. Using a novel Myc-capture GREM1/BMP-4 double sandwich ELISA, the BMP-4 binding capability of both wildtype gremlin-Myc and mutant MGR5 were demonstrated. Moreover, the addition of either soluble unfractionated heparin or the low molecular weight heparin, tinzaparin, in this ELISA neither markedly promoted nor inhibited the interaction between wildtype gremlin-Myc and BMP-4. Overall, these showed that heparin binding is not essential for BMP-gremlin binding. In order to study the functional activities of three selected gremlin-Myc mutants alongside wildtype, their capabilities to inhibit BMP-SMAD1/5/8 signalling were investigated in two functional assays established in C2C12 myoblastic cell lines. Firstly, SMAD1/5/8 phosphorylation was responsive to both BMPs -4 and -7, but this assay failed to generate a graded phospho-SMAD1/5/8 dose response on a sufficiently consistent basis to allow comparison of these gremlin-Myc proteins. This was resolved in the second assay, which employed a SMAD1/5/8-specific luciferase reporter construct. In this reporter assay, the functional activities of concentrated wildtype gremlin-Myc, MGR5 and MGR6 were confirmed.
Subjects/Keywords: gremlin; bone morphogenetic protein; SMAD; CAN family
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APA ·
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APA (6th Edition):
Nkola Tatsinkam, A. J. (2015). The heparin binding properties of the bone morphogenetic protein antagonist gremlin. (Doctoral Dissertation). Royal Holloway, University of London. Retrieved from https://pure.royalholloway.ac.uk/portal/en/publications/the-heparin-binding-properties-of-the-bone-morphogenetic-protein-antagonist-gremlin(ccdab987-aacd-4fad-8a1e-cb952dcd03fa).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792292
Chicago Manual of Style (16th Edition):
Nkola Tatsinkam, Arnold Junior. “The heparin binding properties of the bone morphogenetic protein antagonist gremlin.” 2015. Doctoral Dissertation, Royal Holloway, University of London. Accessed March 08, 2021.
https://pure.royalholloway.ac.uk/portal/en/publications/the-heparin-binding-properties-of-the-bone-morphogenetic-protein-antagonist-gremlin(ccdab987-aacd-4fad-8a1e-cb952dcd03fa).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792292.
MLA Handbook (7th Edition):
Nkola Tatsinkam, Arnold Junior. “The heparin binding properties of the bone morphogenetic protein antagonist gremlin.” 2015. Web. 08 Mar 2021.
Vancouver:
Nkola Tatsinkam AJ. The heparin binding properties of the bone morphogenetic protein antagonist gremlin. [Internet] [Doctoral dissertation]. Royal Holloway, University of London; 2015. [cited 2021 Mar 08].
Available from: https://pure.royalholloway.ac.uk/portal/en/publications/the-heparin-binding-properties-of-the-bone-morphogenetic-protein-antagonist-gremlin(ccdab987-aacd-4fad-8a1e-cb952dcd03fa).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792292.
Council of Science Editors:
Nkola Tatsinkam AJ. The heparin binding properties of the bone morphogenetic protein antagonist gremlin. [Doctoral Dissertation]. Royal Holloway, University of London; 2015. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/the-heparin-binding-properties-of-the-bone-morphogenetic-protein-antagonist-gremlin(ccdab987-aacd-4fad-8a1e-cb952dcd03fa).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792292
Univerzitet u Beogradu
26.
Divnić-Resnik, Tihana. 1976-.
Očuvanje alveolarnog grebena nakon ekstrakcije zuba
primenom koštanog morfogenetskog proteina.
Degree: Stomatološki fakultet, 2018, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:17910/bdef:Content/get
► Tokom zarastanja postekstrakcione rane dolazi do značajnih promena alveolarnog grebena koje se mogu podeliti na unutrašnje i spoljašnje promene u zavisnosti koji deo alveoli zahvataju.…
(more)
▼ Tokom zarastanja postekstrakcione rane dolazi do
značajnih promena alveolarnog grebena koje se mogu podeliti na
unutrašnje i spoljašnje promene u zavisnosti koji deo alveoli
zahvataju. Unutrašnje promene doprinose zarastanju rane i dovode do
formiranja nove kosti u alveoli. Spoljašnje promene su uglavnom
resorptivnog karaktera i dovode do gubitka visine i širine
alveolarnog grebena, što može dalje otežati ugradnju implantata,
kompromitovati protetsko zbrinjavanje pacijenta i estetiku. Do sada
korišćeni terapijski pristupi u cilju prevencijere sorptivnih
promena i očuvanja alveolarnog grebena, nisu pokazali očekivane
rezultate. Preliminarna ispitivanja bioaktivnih supstanci i faktora
rasta i njihove primene bi mogla značajno da uspori procese
resorpcije idoprinese očuvanju alveolarnog
grebena...
Advisors/Committee Members: Aleksić, Zoran, 1970-.
Subjects/Keywords: extraction socket; preservation; bone morphogenetic
protein
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Divnić-Resnik, T. 1. (2018). Očuvanje alveolarnog grebena nakon ekstrakcije zuba
primenom koštanog morfogenetskog proteina. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:17910/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Divnić-Resnik, Tihana 1976-. “Očuvanje alveolarnog grebena nakon ekstrakcije zuba
primenom koštanog morfogenetskog proteina.” 2018. Thesis, Univerzitet u Beogradu. Accessed March 08, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:17910/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Divnić-Resnik, Tihana 1976-. “Očuvanje alveolarnog grebena nakon ekstrakcije zuba
primenom koštanog morfogenetskog proteina.” 2018. Web. 08 Mar 2021.
Vancouver:
Divnić-Resnik T1. Očuvanje alveolarnog grebena nakon ekstrakcije zuba
primenom koštanog morfogenetskog proteina. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Mar 08].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17910/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Divnić-Resnik T1. Očuvanje alveolarnog grebena nakon ekstrakcije zuba
primenom koštanog morfogenetskog proteina. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17910/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Purcell, James.
Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease.
Degree: 2011, RIAN
URL: http://eprints.maynoothuniversity.ie/4216/
► Lung disease and lung injury are responsible for 20% of deaths of the Irish population every year, and the country has the 2nd highest death…
(more)
▼ Lung disease and lung injury are responsible for 20% of deaths of the Irish population every year, and the country has the 2nd highest death rate in Europe for respiratory diseases. Conditions related to the respiratory system are the second largest long term illness by young adults. Lung cancer is the largest cause of cancer related death in Europe as a whole.
New and refined mechanisms of drug delivery for the prevention, cure or delayed progression of disease, represents a pathway for the delivery of novel style therapies and for the targeted delivery of different of more toxic drugs to the airways in order to increase efficiency of both the delivery mechanism and of the drug utilised. Here we looked at the use of a number of different mechanisms, which can be used as stand-alone devices/delivery agents and/or in conjunction with other devices and delivery agents to optimise targeted delivery to the lung, and to the specific areas required.
We examined the use of a direct delivery mechanism, particle bombardment, for the delivery of various molecules to human and murine cells lines, and to mouse primary cell isolates, MAECs, to examine the potential of the mechanism for adaption to a clinical mechanism for delivery. An aerosol delivery system was developed to utilise a current aerosol generator for the delivery of aerosol to mice in vivo. This was done with the aim of creating a more efficacious and ergonomic mechanism for the delivery of aerosols to mice in vivo and also to investigate the effects of aerosolistation on various drug compound molecules.
We also looked for BMP4 disregulation in a number of different animal models to help ascertain the role of the pathway in the progression of disease and damage in the lung. BMP4 has been shown to have a role in the induction of EMT in MAECS (E.Molloy) and to play a role in both lung cancer and allergic Rhinitis. Here we looked at its role in a number of different models. It was firstly examined in vitro in mouse cell lines and primary cell isolates and the effect of pathway stimulation and deregulation examined. The role of the pathway was then examined in both a murine Elastase model of emphysema, where it was determined to be inactive, and a murine OVA model of asthma where deregulation of the active pathway was evident. The pathway was also shown to be activated in a deregulated fashion in an Ozone/HDMA model of allergic asthma in Rhesus Macaques.
In vitro models of mouse, human and primate cells lines were used to examine the role of BMP4 in more detail. Mouse cell lines and primary isolates were used both in normal culture and in an air liquid interface (ALI), stimulated with BMP4 and
examined. An air liquid interface enables the culturing of cells in a system consistent with that of the in vivo environment, where the nutrition is provided through the basal surface of the cells and the dorsal surfaces of the cells are exposed to air. Murine model of OVA induced asthma in vivo was also stimulated with exogenous BMP4 and the effects monitored. Human primary cells…
Subjects/Keywords: Biology; Bone Morphogenetic; Protein Signalling; Lung Disease
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APA (6th Edition):
Purcell, J. (2011). Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease. (Thesis). RIAN. Retrieved from http://eprints.maynoothuniversity.ie/4216/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Purcell, James. “Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease.” 2011. Thesis, RIAN. Accessed March 08, 2021.
http://eprints.maynoothuniversity.ie/4216/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Purcell, James. “Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease.” 2011. Web. 08 Mar 2021.
Vancouver:
Purcell J. Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease. [Internet] [Thesis]. RIAN; 2011. [cited 2021 Mar 08].
Available from: http://eprints.maynoothuniversity.ie/4216/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Purcell J. Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease. [Thesis]. RIAN; 2011. Available from: http://eprints.maynoothuniversity.ie/4216/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
28.
Purcell, James.
Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease.
Degree: 2011, RIAN
URL: http://mural.maynoothuniversity.ie/4216/
► Lung disease and lung injury are responsible for 20% of deaths of the Irish population every year, and the country has the 2nd highest death…
(more)
▼ Lung disease and lung injury are responsible for 20% of deaths of the Irish population every year, and the country has the 2nd highest death rate in Europe for respiratory diseases. Conditions related to the respiratory system are the second largest long term illness by young adults. Lung cancer is the largest cause of cancer related death in Europe as a whole.
New and refined mechanisms of drug delivery for the prevention, cure or delayed progression of disease, represents a pathway for the delivery of novel style therapies and for the targeted delivery of different of more toxic drugs to the airways in order to increase efficiency of both the delivery mechanism and of the drug utilised. Here we looked at the use of a number of different mechanisms, which can be used as stand-alone devices/delivery agents and/or in conjunction with other devices and delivery agents to optimise targeted delivery to the lung, and to the specific areas required.
We examined the use of a direct delivery mechanism, particle bombardment, for the delivery of various molecules to human and murine cells lines, and to mouse primary cell isolates, MAECs, to examine the potential of the mechanism for adaption to a clinical mechanism for delivery. An aerosol delivery system was developed to utilise a current aerosol generator for the delivery of aerosol to mice in vivo. This was done with the aim of creating a more efficacious and ergonomic mechanism for the delivery of aerosols to mice in vivo and also to investigate the effects of aerosolistation on various drug compound molecules.
We also looked for BMP4 disregulation in a number of different animal models to help ascertain the role of the pathway in the progression of disease and damage in the lung. BMP4 has been shown to have a role in the induction of EMT in MAECS (E.Molloy) and to play a role in both lung cancer and allergic Rhinitis. Here we looked at its role in a number of different models. It was firstly examined in vitro in mouse cell lines and primary cell isolates and the effect of pathway stimulation and deregulation examined. The role of the pathway was then examined in both a murine Elastase model of emphysema, where it was determined to be inactive, and a murine OVA model of asthma where deregulation of the active pathway was evident. The pathway was also shown to be activated in a deregulated fashion in an Ozone/HDMA model of allergic asthma in Rhesus Macaques.
In vitro models of mouse, human and primate cells lines were used to examine the role of BMP4 in more detail. Mouse cell lines and primary isolates were used both in normal culture and in an air liquid interface (ALI), stimulated with BMP4 and
examined. An air liquid interface enables the culturing of cells in a system consistent with that of the in vivo environment, where the nutrition is provided through the basal surface of the cells and the dorsal surfaces of the cells are exposed to air. Murine model of OVA induced asthma in vivo was also stimulated with exogenous BMP4 and the effects monitored. Human primary cells…
Subjects/Keywords: Biology; Bone Morphogenetic; Protein Signalling; Lung Disease
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Purcell, J. (2011). Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease. (Thesis). RIAN. Retrieved from http://mural.maynoothuniversity.ie/4216/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Purcell, James. “Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease.” 2011. Thesis, RIAN. Accessed March 08, 2021.
http://mural.maynoothuniversity.ie/4216/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Purcell, James. “Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease.” 2011. Web. 08 Mar 2021.
Vancouver:
Purcell J. Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease. [Internet] [Thesis]. RIAN; 2011. [cited 2021 Mar 08].
Available from: http://mural.maynoothuniversity.ie/4216/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Purcell J. Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease. [Thesis]. RIAN; 2011. Available from: http://mural.maynoothuniversity.ie/4216/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
29.
Chung, Yueh-hua.
BMP-2 Restoration Rescues Liver Fibrosis Injuries by Attenuating TGF-β1 Signaling.
Degree: PhD, Institute of Biomedical Sciences, 2018, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0220118-011954
► Objective: Transforming growth factor-β (TGF-β) plays a central role in hepatic fibrogenesis. This study investigated the function and mechanism of bone morphogenetic protein-2 (BMP-2) in…
(more)
▼ Objective: Transforming growth factor-β (TGF-β) plays a central role in hepatic fibrogenesis. This study investigated the function and mechanism of
bone morphogenetic protein-
2 (BMP-
2) in regulation of hepatic fibrogenesis. Methods: BMP-
2 expression in fibrotic liver was measured in human tissue microarray and mouse models of liver fibrosis induced by bile duct ligation (BDL) surgery or carbon tetrachloride (CCl4) administration. Adenovirus-mediated BMP-
2 gene delivery was used to test the prophylactic effect on liver fibrosis. Primary hepatic stellate cells (HSC) and cell lines, HSC-T6 cells and Clone 9, were used to study the interplay between BMP-
2 and TGF-β1. Results: Hepatic BMP-
2 expression was significantly decreased in either human fibrotic tissues or mice fibrosis models, with a negative correlation with hepatic TGF-β1 contents. BMP-
2 gene delivery alleviated the elevations of serum hepatic enzymes, HSC activation markers, and liver fibrosis in both models. Mechanistically, exogenous TGF-β1 dose-dependently reduced BMP-
2 expression, whereas BMP-
2 significantly suppressed expression of TGF-β and its cognate type I and II receptor peptides, as well as the induced Smad3 phosphorylation levels in primary mouse HSCs. Aside from its suppressive effects on cell proliferation and migration, BMP-
2 treatment prominently attenuated the TGF-β1-stimulated α-smooth muscle actin and fibronectin expression, and reversed the TGF-β1-modulated epithelial-to-mesenchymal transition marker expression in mouse HSCs. Conclusions: The mutual regulation between BMP-
2 and TGF-β1 signaling axes may constitute the anti-fibrogenic mechanism of BMP-
2 in the pathogenesis of liver fibrosis. BMP-
2 may potentially serve as a novel therapeutic target for treatment of liver fibrosis.
Advisors/Committee Members: Ying-Hsien Kao (chair), Ming-Hong Tai (committee member), Kuang-Hung Cheng (chair), Ying-Hsien Huang (chair), Chao-Cheng Huang (chair).
Subjects/Keywords: Transforming growth factor-β1; Hepatic fibrogenesis; Hepatic stellate cells; Bone morphogenetic protein-2; Epithelial-to-mesenchymal transition
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APA ·
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APA (6th Edition):
Chung, Y. (2018). BMP-2 Restoration Rescues Liver Fibrosis Injuries by Attenuating TGF-β1 Signaling. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0220118-011954
Chicago Manual of Style (16th Edition):
Chung, Yueh-hua. “BMP-2 Restoration Rescues Liver Fibrosis Injuries by Attenuating TGF-β1 Signaling.” 2018. Doctoral Dissertation, NSYSU. Accessed March 08, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0220118-011954.
MLA Handbook (7th Edition):
Chung, Yueh-hua. “BMP-2 Restoration Rescues Liver Fibrosis Injuries by Attenuating TGF-β1 Signaling.” 2018. Web. 08 Mar 2021.
Vancouver:
Chung Y. BMP-2 Restoration Rescues Liver Fibrosis Injuries by Attenuating TGF-β1 Signaling. [Internet] [Doctoral dissertation]. NSYSU; 2018. [cited 2021 Mar 08].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0220118-011954.
Council of Science Editors:
Chung Y. BMP-2 Restoration Rescues Liver Fibrosis Injuries by Attenuating TGF-β1 Signaling. [Doctoral Dissertation]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0220118-011954
University of Minnesota
30.
Tasca, Amy.
The Role of Myosin X in BMPs Promotion of SMAD 1/5 in Osteoclast Formation and Function.
Degree: PhD, Oral Biology, 2017, University of Minnesota
URL: http://hdl.handle.net/11299/200247
► Novel therapeutic strategies that target osteoclasts are needed for many orthopedic, craniofacial and cancer applications. Incomplete understanding of the factors that regulate osteoclasts limits this…
(more)
▼ Novel therapeutic strategies that target osteoclasts are needed for many orthopedic, craniofacial and cancer applications. Incomplete understanding of the factors that regulate osteoclasts limits this development. Currently, bone morphogenetic proteins (BMPs) are used to promote healing of open fractures, bone grafts and non-union fractures. Bone healing requires synthesis of new bone by osteoblasts and the removal of old or damaged bone matrix by osteoclasts. Although BMPs have been widely studied as stimulators of osteoblastic bone formation, it has also been shown that BMPs directly increase osteoclast formation, however the mechanism behind this enhancement is not completely understood. Two potential candidates responsible for BMPs enhancement of osteoclast formation could be SMADs, components of the canonical BMP signaling pathway, and MYO10, a regulator of the osteoclast cytoskeleton. The data presented in this thesis demonstrates that loss of SMAD1/5 expression by osteoclasts in in vitro cultures of osteoclasts inhibits osteoclast differentiation and activity. However, the loss of SMAD1/5 expression in osteoclasts and macrophages in a mouse model leads to enhance osteoclast differentiation and activity. Interestingly bone formation is also increased in the mouse model with decreased SMAD1/5 expression suggesting that SMAD1/5 signaling in osteoclasts may regulate coupling between osteoclast and osteoblast activity. Lastly MYO10 besides playing a role in osteoclast activity also plays a role in regulating osteoclast differentiation. Data presented demonstrates that loss of MYO10 expression correlates with changes in the osteoclast cytoskeleton which impacts the ability of osteoclasts to fuse. By gaining knowledge of regulators of osteoclastogenesis and the mechanism by which they modulate osteoclast differentiation and activity, we will better understand the etiology of osteoclast diseases and provide novel insight into future therapies.
Subjects/Keywords: Bone Formation; Bone Morphogenetic Protein; Bone Resorption; Myosin X; Osteoclast
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Tasca, A. (2017). The Role of Myosin X in BMPs Promotion of SMAD 1/5 in Osteoclast Formation and Function. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/200247
Chicago Manual of Style (16th Edition):
Tasca, Amy. “The Role of Myosin X in BMPs Promotion of SMAD 1/5 in Osteoclast Formation and Function.” 2017. Doctoral Dissertation, University of Minnesota. Accessed March 08, 2021.
http://hdl.handle.net/11299/200247.
MLA Handbook (7th Edition):
Tasca, Amy. “The Role of Myosin X in BMPs Promotion of SMAD 1/5 in Osteoclast Formation and Function.” 2017. Web. 08 Mar 2021.
Vancouver:
Tasca A. The Role of Myosin X in BMPs Promotion of SMAD 1/5 in Osteoclast Formation and Function. [Internet] [Doctoral dissertation]. University of Minnesota; 2017. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/11299/200247.
Council of Science Editors:
Tasca A. The Role of Myosin X in BMPs Promotion of SMAD 1/5 in Osteoclast Formation and Function. [Doctoral Dissertation]. University of Minnesota; 2017. Available from: http://hdl.handle.net/11299/200247
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Open Access Theses and Dissertations
