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Wake Forest University
1.
Taylor, Alexander David.
PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE.
Degree: 2016, Wake Forest University
URL: http://hdl.handle.net/10339/64180 
► The basis of plasmonic sensors is the resonant coupling between the oscillations of free electrons, called plasmons, and incident visible light waves. By confining these…
(more)
▼ The basis of plasmonic sensors is the resonant coupling between the oscillations of free electrons, called plasmons, and incident visible light waves. By confining these oscillations within a nanostructure, the coupling efficiency is enhanced by the creation of localized surface plasmon resonant (LSPR) states. The frequency at which these oscillations occur is dependent upon a number of factors, one of which is the proximity of another plasmonic nanoparticle. The relationship between the frequency of the LSPR oscillations and the distance separating the nanoparticles is called the plasmon nanoruler (PNR). This phenomenon is highly distance dependent - a measurement of the LSPR for a plasmonic nanoparticle allows a researcher to calculate the interparticle separation for length scales well beneath the diffraction limit for visible light. However, even with the enhanced coupling between the nanoparticle and incident light, the signal from a single nanoruler is very dim, and adequate control over many nanorulers is difficult to achieve.
Subjects/Keywords: Biosensors
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APA (6th Edition):
Taylor, A. D. (2016). PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/64180
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Taylor, Alexander David. “PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE.” 2016. Thesis, Wake Forest University. Accessed February 26, 2021.
http://hdl.handle.net/10339/64180.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Taylor, Alexander David. “PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE.” 2016. Web. 26 Feb 2021.
Vancouver:
Taylor AD. PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE. [Internet] [Thesis]. Wake Forest University; 2016. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10339/64180.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Taylor AD. PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE. [Thesis]. Wake Forest University; 2016. Available from: http://hdl.handle.net/10339/64180
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Bath
2.
Ustuner, Serife.
Electrochemical detection of microRNAs for cancer diagnosis.
Degree: PhD, 2020, University of Bath
URL: https://researchportal.bath.ac.uk/en/studentthesis/electrochemical-detection-of-micrornas-for-cancer-diagnosis(7c1ec5f2-4c7f-45cf-8812-8a852840c9f2).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.814324
► Cancer continues to grow globally, exerting serious financial and emotional strain on individuals, families, associations and health systems. Early detection is crucial in order to…
(more)
▼ Cancer continues to grow globally, exerting serious financial and emotional strain on individuals, families, associations and health systems. Early detection is crucial in order to control the spread of the disease and improve chances of survival. Despite the significant intellectual and financial efforts worldwide, there are currently no blood based biomarkers that are suitable for the non-invasive early detection of cancer. MicroRNAs/miRNAs are a family of non-protein-coding small RNAs which regulate the expression of nearly one third of all human genes. Over the past decade, significant evidence has emerged showing that miRNAs and modulation of their levels in human body are involved in the pathogenesis of cancer at its early stages. The main consideration when selecting methods of detection for miRNAs is the requirement for high-sensitivity and selectivity due to their low concentrations in blood. This thesis investigates a new method of electrochemical detection which is distinctive due to its sensitivity together with its simple, rapid and reliable design. The first experimental study investigates the role of artificial oligonucleotide probes for selective recognition of miRNAs. Upon using Peptide Nucleic Acid (PNA) probes, highly-sensitive and direct electrochemical detection of a pancreatic cancer-specific miRNA has been achieved with no additional labelling steps. The second study examines the adoption of magnetic beads for separation of target miRNAs from complex solutions (e.g. blood). This strategy addresses the major problem of nonspecific interactions that arises upon direct subjection of sensor surfaces to complex solutions. The final study concentrates on the use of redox active self-assembled monolayers (SAMs) on electrode surface for direct capacitive-based detection of miRNAs. Such methodology eliminates the need for pre-doping of the measurement’s solution or labelling of target and is highly desirable characteristic for a point-of-care device to be used for the analysis of clinical samples. Together, this thesis aims to serve as a potential guidance for overcoming the shortcomings of the current miRNA sensing, add value to the development of oligonucleotide-based biosensors for detection of circulating miRNA and hopefully enable the early detection of cancer state.
Subjects/Keywords: Biosensors
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APA (6th Edition):
Ustuner, S. (2020). Electrochemical detection of microRNAs for cancer diagnosis. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/electrochemical-detection-of-micrornas-for-cancer-diagnosis(7c1ec5f2-4c7f-45cf-8812-8a852840c9f2).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.814324
Chicago Manual of Style (16th Edition):
Ustuner, Serife. “Electrochemical detection of microRNAs for cancer diagnosis.” 2020. Doctoral Dissertation, University of Bath. Accessed February 26, 2021.
https://researchportal.bath.ac.uk/en/studentthesis/electrochemical-detection-of-micrornas-for-cancer-diagnosis(7c1ec5f2-4c7f-45cf-8812-8a852840c9f2).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.814324.
MLA Handbook (7th Edition):
Ustuner, Serife. “Electrochemical detection of microRNAs for cancer diagnosis.” 2020. Web. 26 Feb 2021.
Vancouver:
Ustuner S. Electrochemical detection of microRNAs for cancer diagnosis. [Internet] [Doctoral dissertation]. University of Bath; 2020. [cited 2021 Feb 26].
Available from: https://researchportal.bath.ac.uk/en/studentthesis/electrochemical-detection-of-micrornas-for-cancer-diagnosis(7c1ec5f2-4c7f-45cf-8812-8a852840c9f2).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.814324.
Council of Science Editors:
Ustuner S. Electrochemical detection of microRNAs for cancer diagnosis. [Doctoral Dissertation]. University of Bath; 2020. Available from: https://researchportal.bath.ac.uk/en/studentthesis/electrochemical-detection-of-micrornas-for-cancer-diagnosis(7c1ec5f2-4c7f-45cf-8812-8a852840c9f2).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.814324
Oregon State University
3.
Hutchison, Janine R.
Bacterial pathogens and associated toxins involved in erythrophore cell aggregation.
Degree: PhD, Microbiology, 2009, Oregon State University
URL: http://hdl.handle.net/1957/11976
► Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable,…
(more)
▼ Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as a method to detect toxic agents such as chemicals and toxins.
The work described here, investigated the potential of erythrophore cells as a detection method for the model food-associated pathogenic bacteria Salmonella enteritidis, Clostridium perfringens, Clostridium botulinum and Bacillus cereus. Erythrophore cell response to each bacterial pathogen was unique and could be distinguished from the bacterial growth medium. These results demonstrate that erythrophore cell response can be used to detect a set of pathogenic bacteria.
A second focus of this research effort was to test the hypothesis that erythrophore cells respond to pathogenic bacteria differently then nonpathogenic bacteria. To test this hypothesis, erythrophore cells were exposed to pathogenic and nonpathogenic Bacillus strains. Erythrophore cells failed to respond to Bacillus subtilis and a nonpathogenic Bacillus anthracis vaccine strain. Erythrophore cells responded to members of the B. cereus group including: B. cereus ATCC 10987, B. cereus UW85, B. cereus ATCC 14579, emetic B. cereus NCTC 11143, and Bacillus thuringiensis subsp. kurstaki BGSC 4D1.
A third focus of this research effort was to identify the bacterial virulence factor(s) responsible for inducing erythrophore cell response. Random chemical mutagenesis of B. cereus ATCC 49064 resulted in a single amino acid conversion of alanine to threonine at residue 289 of L2 toxin component of HBL. This mutation resulted in a delayed erythrophore cell response which was complemented with the wildtype hbl gene implementing the HBL toxin complex as a factor capable of inducing erythrophore cell response.
In conclusion, this study demonstrates that erythrophore cells can be optically monitored to detect several different bacterial pathogens. From this work, the utility of erythrophore cell response can be expanded from function based detection to include studying toxin activity as well as how pathogenic bacteria interact with eukaryotic cells.
Advisors/Committee Members: Trempy, Janine E. (advisor), Bermudez, Luiz (committee member).
Subjects/Keywords: Chromatophore; Biosensors
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APA (6th Edition):
Hutchison, J. R. (2009). Bacterial pathogens and associated toxins involved in erythrophore cell aggregation. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/11976
Chicago Manual of Style (16th Edition):
Hutchison, Janine R. “Bacterial pathogens and associated toxins involved in erythrophore cell aggregation.” 2009. Doctoral Dissertation, Oregon State University. Accessed February 26, 2021.
http://hdl.handle.net/1957/11976.
MLA Handbook (7th Edition):
Hutchison, Janine R. “Bacterial pathogens and associated toxins involved in erythrophore cell aggregation.” 2009. Web. 26 Feb 2021.
Vancouver:
Hutchison JR. Bacterial pathogens and associated toxins involved in erythrophore cell aggregation. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1957/11976.
Council of Science Editors:
Hutchison JR. Bacterial pathogens and associated toxins involved in erythrophore cell aggregation. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/11976
Oregon State University
4.
Dukovcic, Stephanie R.
Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances.
Degree: PhD, Microbiology, 2009, Oregon State University
URL: http://hdl.handle.net/1957/12924
► Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application…
(more)
▼ Cell-based
biosensors are function-based detectors that use the physiological
response of a living cell to sense biologically stimulating agents. This emerging
technology extends the application of current detection methods by reporting on the
toxicity of a sample and the potential to cause disease. Previously, Betta splendens
erythrophores have been described as a method to detect toxic agents such as
pesticides, chemicals, purified bacterial toxins and food-associated bacteria.
The first objective of this study was to examine the B. splendens erythrophore
response to Gram-negative food-associated bacteria by investigating the response to
Salmonella typhimurium. Erythrophores aggregated in the presence of S. typhimurium
in a growth-phase-dependent manner and this response was distinct from the media
control. Additionally, it was found that erythrophore aggregation was dependent on
the bacterial cell and erythrophores were not responsive to secreted bacterial products
found in the culture supernatant. Transposon mutagenesis of S. typhimurium ATCC
700720 resulted in an interruption of the promoter region of operon prgHIJK. This
insertion inactivated the expression of the SPI-1 T3SS structural proteins. The
mutation resulted in a delayed erythrophore aggregation, implying that the SPI-1
T3SS is an important component contributing to erythrophore aggregation.
The second objective of this study was to characterize the Oncorhynchus
tshawytscha melanophore in terms of its responsiveness to aggregative and dispersive
controls. It was hypothesized that the pigment response was biologically conserved
between O. tshawytscha melanophores and B. splendens erythrophores. The data
presented support this hypothesis and highlight potential applications. Melanophores
aggregated in response to mercuric chloride and sodium arsenite but dispersed in the
presence of ammonia. Additionally, melanophores aggregated in response to
salmonid bacterial pathogens Aeromonas salmonicida, Yersinia ruckeri and
Flavobacterium psychrophilum. Melanophores were capable of differentiating
between healthy and diseased fish tissues. These results warrant a continued
investigation into the full potential of the O. tshawytscha melanophore system.
In conclusion, this study investigated two cell-based biosensor models; B.
splendens erythrophores and O. tshawytscha melanophores. The data presented
suggests that these models can be applied for the detection of foodborne bacterial
pathogens and for the detection of chemical and bacterial contaminants pertinent to
the aquaculture industry.
Advisors/Committee Members: Trempy, Janine E. (advisor), Lowry, Malcolm B. (committee member).
Subjects/Keywords: Chromatophore; Biosensors
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APA (6th Edition):
Dukovcic, S. R. (2009). Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/12924
Chicago Manual of Style (16th Edition):
Dukovcic, Stephanie R. “Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances.” 2009. Doctoral Dissertation, Oregon State University. Accessed February 26, 2021.
http://hdl.handle.net/1957/12924.
MLA Handbook (7th Edition):
Dukovcic, Stephanie R. “Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances.” 2009. Web. 26 Feb 2021.
Vancouver:
Dukovcic SR. Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1957/12924.
Council of Science Editors:
Dukovcic SR. Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/12924
Oregon State University
5.
Chalishazar, Vishvas.
Nanostructured Optical Devices for Biosensing Applications.
Degree: MS, Electrical and Computer Engineering, 2016, Oregon State University
URL: http://hdl.handle.net/1957/58140
► We demonstrated a sensitive biosensor based on plasmonic metal composite nanostructures. The device can be fabricated and duplicated with ease using a novel nanoprinting technology…
(more)
▼ We demonstrated a sensitive biosensor based on plasmonic metal composite nanostructures. The device can be fabricated and duplicated with ease using a novel nanoprinting technology which allows precise transfer of metal nanostructures without complicated lithography processes. The design featured by its unique material composition and sub-wavelength structures maximizes the exposure of localized plasmonic hot spots to analyte and, therefore, enhances the refractive-index sensitivity with a plasmon resonance peak in visible range. A refractive-index-sensitivity of ~ 502 nm/RIU and a figure-of-merit of ~ 30.1 RIU⁻¹ has been achieved. The new sensing technology will find applications in medical diagnostics and environmental monitoring.
Advisors/Committee Members: Cheng, Li Jing (advisor), Brekken, Ted (committee member).
Subjects/Keywords: Nanostructure; Biosensors
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APA (6th Edition):
Chalishazar, V. (2016). Nanostructured Optical Devices for Biosensing Applications. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/58140
Chicago Manual of Style (16th Edition):
Chalishazar, Vishvas. “Nanostructured Optical Devices for Biosensing Applications.” 2016. Masters Thesis, Oregon State University. Accessed February 26, 2021.
http://hdl.handle.net/1957/58140.
MLA Handbook (7th Edition):
Chalishazar, Vishvas. “Nanostructured Optical Devices for Biosensing Applications.” 2016. Web. 26 Feb 2021.
Vancouver:
Chalishazar V. Nanostructured Optical Devices for Biosensing Applications. [Internet] [Masters thesis]. Oregon State University; 2016. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1957/58140.
Council of Science Editors:
Chalishazar V. Nanostructured Optical Devices for Biosensing Applications. [Masters Thesis]. Oregon State University; 2016. Available from: http://hdl.handle.net/1957/58140
Oregon State University
6.
Roussel, Nicolas.
Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents.
Degree: MS, Electrical and Computer Engineering, 2003, Oregon State University
URL: http://hdl.handle.net/1957/30351
► Image segmentation is the process by which an image is divided into number of regions. The regions are to be homogeneous with respect to some…
(more)
▼ Image segmentation is the process by which an image is divided into number of
regions. The regions are to be homogeneous with respect to some property. Definition
of homogeneity depends mainly on the expected patterns of the objects of interest. The
algorithms designed to perform these tasks can be divided into two main families: Splitting
Algorithms and Merging Algorithms. The latter comprises seeded region growing
algorithms which provide the basis for our work.
Seeded region growing methods such as Marker initiated Watershed segmentation
depend principally on the quality and relevance of the initial seeds. In situations where
the image contains a variety of aggregated objects of different shapes, finding reliable
initial seeds can be a very complex task.
This thesis describes a versatile approach for finding initial seeds on images featuring
objects distinguishable by their structural and intensity profiles. This approach
involves the use of hierarchical trees containing various information on the objects in
the image. These trees can be searched for specific pattern to generate the initial seeds
required to perform a reliable region growing process. Segmentation results are shown
in this thesis.
The above image segmentation scheme has been applied to detect isolated living
cells in a sequence of frames and monitor their behavior through the time. The tissues
utilized for these studies are isolated from the scales of Betta Splendens fish family.
Since the isolated cells or chromatophores are sensitive to various kinds of toxic agents,
a creation of cell-based toxin detector was suggested. Such sensor operation depends on
an efficient segmentation of cell images and extraction of pertinent visual features.
Our ultimate objective is to model and classify the observed cell behavior in order
to detect and recognize biological or chemical agents affecting the cells. Some possible
modelling and classification approaches are presented in this thesis.
Advisors/Committee Members: Kolodziej, Wojtek J. (advisor).
Subjects/Keywords: Biosensors
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APA (6th Edition):
Roussel, N. (2003). Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/30351
Chicago Manual of Style (16th Edition):
Roussel, Nicolas. “Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents.” 2003. Masters Thesis, Oregon State University. Accessed February 26, 2021.
http://hdl.handle.net/1957/30351.
MLA Handbook (7th Edition):
Roussel, Nicolas. “Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents.” 2003. Web. 26 Feb 2021.
Vancouver:
Roussel N. Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1957/30351.
Council of Science Editors:
Roussel N. Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/30351
7.
Karapetis, stefanos.
Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό.
Degree: 2019, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ)
URL: http://hdl.handle.net/10442/hedi/45413
► The biosensors have important role in science of chemical analysis in the field of electrochemical methods. Their application in rapid detection of toxins in types,…
(more)
▼ The biosensors have important role in science of chemical analysis in the field of electrochemical methods. Their application in rapid detection of toxins in types, we consume in our daily lives, is particularly useful for public health. The main characteristics of evaluation of biosensors is the high sensitivity, high selectivity, the quick response and the reusability. Analysis of the real samples need additionally a method of evaluating the rate of recovery that is the fractional difference of signal between real and simulated samples. The advantages of the use of biosensors are part of the easy construction, portability and low cost. The provision of analytical results of biosensors is similar to that of other far more expensive methods of instrumental chemical analysis.In this thesis developed a principle flow injection biosensor (velocity at 2 ml/min) for detection of cholera toxin in aqueous fractions of samples (10 μL or 20 μL).). The biosensor constructed by depositing copper-coated graphene nanoparticles with integrated lipid membranes (BLMs), modified with native GM1 receptors. In the proposed biosensor, regeneration was checked every 6 minutes with 10 successful regenerations retaining its original potency. Control of the biosensor in simulated samples of lake waters was successful and no significant changes were observed under the influence of 7 conventional inhibitors introduced into the samples. The appropriate environment to perform the measurements was set at pH = 7. The biosensor was tested in a dynamic range of the toxin (10nM-10μM) and the detection limit was determined to (LOD = 1nM). This biosensor was studied also in 10 samples of real lake water and the recovery rate was calculated at two concentrations of Toxin (Low 50 nM and High 7 μM). The recovery rates found are acceptable according to the ICH2005 criteria for the suitability of the biosensor for use in repeated water monitoring.Three standard aptasensors were then developed to detect Aflatoxin (AFM1) in milk. The first Label-free aptasensor was made by surface deposition of dendrimer (PAMAM-4G) on a gold electrode where an amino-transformed aptamer -receptor was immobilized. This aptasensor was used as an electrode in the instrumental analysis of the electrochemical impedance spectroscopy (EIS). Control was performed on simulated milk samples at a dynamic range of toxin concentration (15-120 ng/l) and the toxin detection limit was determined at (LOD = 8.47 ng/l). Also, the aptasensor tested in real samples at the same dynamic range with a similar limit of detection and signal recovery greater than 78%.The second aptasensor was made by surface deposition of Neutravidin/Fe-COOH on a gold electrode, where a modified aptamer-receptor with biotin was immobilized. This aptasensor was used as an electrode in the instrumental method of Differential Pulse Voltammetry (DPV). Control was performed on simulated milk samples at a dynamic range of toxin concentration (15-120 ng / l) and the toxin limit of detection was determined at (LOD = 8.52 ng /…
Subjects/Keywords: Βιοαισθητήρες; Biosensors
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APA (6th Edition):
Karapetis, s. (2019). Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό. (Thesis). National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Retrieved from http://hdl.handle.net/10442/hedi/45413
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Karapetis, stefanos. “Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό.” 2019. Thesis, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Accessed February 26, 2021.
http://hdl.handle.net/10442/hedi/45413.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Karapetis, stefanos. “Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό.” 2019. Web. 26 Feb 2021.
Vancouver:
Karapetis s. Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό. [Internet] [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2019. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10442/hedi/45413.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Karapetis s. Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό. [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2019. Available from: http://hdl.handle.net/10442/hedi/45413
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Michigan State University
8.
Znati, Cynthia Ann.
Feasibility study of a biosensor for biological processes.
Degree: MS, Department of Chemical Engineering, 1991, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:22389
Subjects/Keywords: Biosensors
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APA (6th Edition):
Znati, C. A. (1991). Feasibility study of a biosensor for biological processes. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:22389
Chicago Manual of Style (16th Edition):
Znati, Cynthia Ann. “Feasibility study of a biosensor for biological processes.” 1991. Masters Thesis, Michigan State University. Accessed February 26, 2021.
http://etd.lib.msu.edu/islandora/object/etd:22389.
MLA Handbook (7th Edition):
Znati, Cynthia Ann. “Feasibility study of a biosensor for biological processes.” 1991. Web. 26 Feb 2021.
Vancouver:
Znati CA. Feasibility study of a biosensor for biological processes. [Internet] [Masters thesis]. Michigan State University; 1991. [cited 2021 Feb 26].
Available from: http://etd.lib.msu.edu/islandora/object/etd:22389.
Council of Science Editors:
Znati CA. Feasibility study of a biosensor for biological processes. [Masters Thesis]. Michigan State University; 1991. Available from: http://etd.lib.msu.edu/islandora/object/etd:22389
Massey University
9.
Goh, Leong Peng.
The development of amperometric biosensors for the detection of glucose, lactate and ethanol.
Degree: MS, Biochemistry, 1996, Massey University
URL: http://hdl.handle.net/10179/10544
► Amperometric biosensors, also commonly known as enzyme sensors or enzyme electrodes, are a growing and very progressive area of research. Biosensors are analytical devices that…
(more)
▼ Amperometric biosensors, also commonly known as enzyme sensors or enzyme electrodes, are a growing and very progressive area of research. Biosensors are analytical devices that contain a biological sensing element connected to a physical transducing element. The physical transducer "senses" the change in the biological element as it undergoes a chemical reaction. The physical transducer then converts chemical equivalents from the enzyme reaction in a dependent relationship to electrical equivalents that can be measured. Biosensors combine the power of electrochemistry with the specificity of enzymes to produce sensors that are specific to particular enzyme substrates. Some have wide specificities and others are quite narrow. Considering the wide range of enzymes available, the choice depends on the end use of these sensors. The aim of the current study was to design biosensors for the detection of glucose, lactate and ethanol. The method for attaching enzymes to electrodes was based on the carbodiimide method. The carbodiimide method activates haeme which then is able to be covalently attached to enzymes. Enzyme-haeme conjugates were then allowed to absorb onto platinum electrodes by exploiting the knowledge that haeme can bind irreversibly to platinum by sharing pi-electrons with the d-orbitals of platinum. The enzymes involved were glucose oxidase, lactate dehydrogenase and alcohol dehydrogenase. The use of flow injection analysis for evaluating biosensors was desenbed and was found to be a fast, efficient method and the results were highly reproducible. In testing electrodes, the results of the present study showed it was possible to obtain current response that was dependent on the concentration of substrate when these enzyme electrodes were used. A particularly significant result in this study was the achievement of current responses that were dependent on substrate concentration in the absence of NAD+ for lactate and alcohol dehydrogenases using the substrates lactate and ethanol respectively. There is however much work to be done to improve the success rate of making these enzyme electrodes. Several factors were found to cause variable results whilst making and using these enzyme electrodes, such as the absorption of unbound enzyme to the sensing surface of the electrode that may produce significant current response, the formation of aggregated haeme during the enzyme-haeme conjugation process and most importantly, and the ability to make successful enzyme-haeme conjugates to be absorbed onto the sensing surface of the electrodes.
Subjects/Keywords: Biosensors
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APA (6th Edition):
Goh, L. P. (1996). The development of amperometric biosensors for the detection of glucose, lactate and ethanol. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/10544
Chicago Manual of Style (16th Edition):
Goh, Leong Peng. “The development of amperometric biosensors for the detection of glucose, lactate and ethanol.” 1996. Masters Thesis, Massey University. Accessed February 26, 2021.
http://hdl.handle.net/10179/10544.
MLA Handbook (7th Edition):
Goh, Leong Peng. “The development of amperometric biosensors for the detection of glucose, lactate and ethanol.” 1996. Web. 26 Feb 2021.
Vancouver:
Goh LP. The development of amperometric biosensors for the detection of glucose, lactate and ethanol. [Internet] [Masters thesis]. Massey University; 1996. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10179/10544.
Council of Science Editors:
Goh LP. The development of amperometric biosensors for the detection of glucose, lactate and ethanol. [Masters Thesis]. Massey University; 1996. Available from: http://hdl.handle.net/10179/10544
Massey University
10.
Large, Ruth.
Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University
.
Degree: 1993, Massey University
URL: http://hdl.handle.net/10179/14730
► The aim of the following work was to design a biosensor for the detection of ethanol. A biosensor is an analytical device in which a…
(more)
▼ The aim of the following work was to design a biosensor for the detection of ethanol. A biosensor is an analytical device in which a biological sensing element is connected to or integrated with a physical transducing element. Amperometric enzyme biosensors utilise one or more enzymes to convert a substance which cannot be measured electrochemically to one which can be. In the case of an alcohol biosensor one of two enzymes (alcohol dehydrogenase and alcohol oxidase) can be used to convert electrochemically stable alcohol to either hydrogen peroxide or NADH which can be oxidised.
In the design of an alcohol biosensor there are three major variables to consider, these are; enzyme type, electrode material, and immobilisation technique. The goal was to select optimum conditions for the formulation of the desired sensor. In the present work the electrode materials used were platinum, carbon (foil and paste) and the conducting organic salt N-methyl phenazinium.Tetracyanoquinodimethane (NMP.TCNQ). The immobilisation techniques used were; adsorption, cross-linking to a protein matrix and covalent binding.
Of the biosensors produced from a selected combination or these variables each was tested by one or more of the following; cyclic voltammetry, enzyme assay, and amperometry. The most promising approach appears to be that of conjugating enzyme to haemin and allowing the conjugate to bind irreversibly to platinum via the haemin group. An electrode made with the organic salt NMP.TCNQ looked promising also but because the salt is readily oxidised it is unstable and therefore not an ideal electrode material.
Subjects/Keywords: Biosensors
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APA (6th Edition):
Large, R. (1993). Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University
. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/14730
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Large, Ruth. “Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University
.” 1993. Thesis, Massey University. Accessed February 26, 2021.
http://hdl.handle.net/10179/14730.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Large, Ruth. “Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University
.” 1993. Web. 26 Feb 2021.
Vancouver:
Large R. Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University
. [Internet] [Thesis]. Massey University; 1993. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10179/14730.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Large R. Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University
. [Thesis]. Massey University; 1993. Available from: http://hdl.handle.net/10179/14730
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rutgers University
11.
Prakash, Shreya, 1994-.
A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.
Degree: MS, Electrical and Computer Engineering, 2019, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/
► Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample…
(more)
▼ Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample volume and reduced cost per test. Currently, Flow Cytometers are used for blood cells enumeration, however, they are expensive, requires fluorescent tagging of cells and trained technicians to operate the instrument. We propose a non-fluorescent microfluidic architecture with single excitation and detection scheme using the barcoded particles fabricated using Stop Flow Lithography process. The barcoded particles designed with specific number of coding regions can generate numerous distinct patterns of electrical signatures and can be used in biological assays to detect multiple blood cells.
A novel design of the asymmetric rectangular barcoded particle was proposed and tested in COMSOL Multiphysics. The barcoded particle with five coding regions generated distinct bi-polar signatures in the microfluidic impedance detection system. Different configurations of the sensing system were proposed to detect the conjugated microspheres (representative of blood cells) effectively based on the site of conjugation. The bottom co-planar electrode and top-bottom electrode configurations were tested for sensitive detection of conjugated microspheres to the barcoded particle. We found that our microfluidic detection system was sensitive enough to detect the presence of a microsphere attached to the barcoded particle. Further, we also investigated the effect of microspheres conjugation orientation to the barcoded particle and their associated electrical signatures. We developed a multi-feature selection algorithm to solve the orientation problem and improved the accuracy of our sensing mechanism. Finally, we fabricated the microfluidic chips using lithography, and co-planar electrodes on glass surfaces using thin film deposition process in the clean room. Our proposed microfluidic system can enumerate multiple blood cells in a single assay using barcoded particles. The concentration of these cells provides useful information about disease onset and progression. Such sensors can be used for diagnostic and management of diseases like Sepsis, Acute Kidney Injury, HIV/ AIDS.
Advisors/Committee Members: Hassan, Umer (chair), Gajic, Zoran (internal member), Wu, Chung-Tse Michael (internal member), School of Graduate Studies.
Subjects/Keywords: Microfluidic; Biosensors
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APA (6th Edition):
Prakash, Shreya, 1. (2019). A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61911/
Chicago Manual of Style (16th Edition):
Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Masters Thesis, Rutgers University. Accessed February 26, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.
MLA Handbook (7th Edition):
Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Web. 26 Feb 2021.
Vancouver:
Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Feb 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.
Council of Science Editors:
Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/
Cornell University
12.
Matlock-Colangelo, Lauren.
Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems.
Degree: M.S., Agricultural and Biological Engineering, Agricultural and Biological Engineering, 2012, Cornell University
URL: http://hdl.handle.net/1813/29389
► Biosensors detect target analytes through specific binding with biological recognition elements such as nucleic acids, enzymes, and antibodies. Many labs are working to create inexpensive…
(more)
▼ Biosensors detect target analytes through specific binding with biological recognition elements such as nucleic acids, enzymes, and antibodies. Many labs are working to create inexpensive and portable miniaturized sensors that allow for rapid sample analysis and low reagent consumption in order to increase biosensor accessibility in rural areas and third world countries. Lab-on-a-chip devices aim to incorporate sample preparation and analyte detection into one device in order to create self-contained sensors that can be used in rural areas and third world countries where laboratory equipment may not be available. Often, these devices incorporate microfluidics in order to shorten reaction times, reduce handling of hazardous samples, and take advantage of laminar flow [1]. However, while several successful lab-on-achip devices have been developed, incorporating sample preparation and analyte detection within one device is still a key challenge in the design of many
biosensors. Sample preparation is extremely important for miniaturized sensors, which have a low tolerance for sample impurities and particulates [1]. In addition, significant sample concentration is often required to reduce sample volumes to the nL to mL range used in miniaturized sensors. This research aims to address the need for sample preparation within lab-on-a-chip systems through the use of functionalized electrospun nanofibers within polymer microfluidic devices. Electrospinning is a fiber formation process that uses electrical forces to form fibers with diameters on the order of 100 nm from polymer spinning dopes [2, 3]. The non-woven fiber mats formed during electrospinning have extremely high surface area to volume ratios, and can be used to increase the sensitivity and binding capacity of
biosensors without increasing their size. Additionally, the fibers can be functionalized through the incorporation of nano and microscale materials within a polymer spinning dope. In this work, positively and negatively charged v nanofibers were created through the incorporation of hexadimethrine bromide (polybrene) and poly(maleic anhydride) (Poly(MA)) within a poly(vinyl alcohol) spinning dope. Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the successful incorporation of polybrene and poly(MA) into the nanofibers. Gold microelectrodes were patterned on poly(methyl methacrylate) (PMMA) to facilitate the incorporation of nanofibers within microfluidic devices. The gold microelectrodes served as grounded collector plates during electrospinning and produced well-aligned nanofiber mats. Microchannels 1 mm wide and 52 [MICRO SIGN]m deep were imprinted into PMMA through hot embossing with a copper template. PMMA pieces embossed with microchannels were bonded to PMMA pieces with gold microelectrodes and nanofibers using UV-assisted thermal bonding. Positively charged polybrene-modified nanofibers were shown to successfully filter negatively charged fluorescent liposomes out of a HEPES-sucrose-saline buffer, while…
Advisors/Committee Members: Baeumner, Antje J (chair), Kirby, Brian (committee member), Frey, Margaret W (committee member).
Subjects/Keywords: Biosensors; Nanofibers; Microfluidics
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APA (6th Edition):
Matlock-Colangelo, L. (2012). Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems. (Masters Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/29389
Chicago Manual of Style (16th Edition):
Matlock-Colangelo, Lauren. “Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems.” 2012. Masters Thesis, Cornell University. Accessed February 26, 2021.
http://hdl.handle.net/1813/29389.
MLA Handbook (7th Edition):
Matlock-Colangelo, Lauren. “Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems.” 2012. Web. 26 Feb 2021.
Vancouver:
Matlock-Colangelo L. Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems. [Internet] [Masters thesis]. Cornell University; 2012. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1813/29389.
Council of Science Editors:
Matlock-Colangelo L. Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems. [Masters Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/29389
Rhodes University
13.
Fogel, Ronen.
Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors.
Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2011, Rhodes University
URL: http://hdl.handle.net/10962/d1007166
► Given their widespread effects and distribution in both natural and industrial environments, the monitoring of phenolic compounds is of considerable analytical interest. Electrochemical biosensor technologies,…
(more)
▼ Given their widespread effects and distribution in both natural and industrial environments, the monitoring of phenolic compounds is of considerable analytical interest. Electrochemical biosensor technologies, in particular those comprising laccase enzymes, afford many potential benefits to address this analytical need. However, several key factors affecting sensor response currently limit their applicability. This Thesis reports on the fabrication and optimisation of an electrochemical laccase-based biosensor towards the application of the monitoring of phenolic compounds. Selected factors considered to affect sensor response were investigated using the optimised biosensor. These included: electrochemical, biochemical and substrate-dependent factors, which were found to intersect in modulating biosensor response signals. Through the application of transducer-dependent and substrate-dependent parameters, the selective and simultaneous detection of a mixture of different phenolic analytes is successfully demonstrated. This Thesis also investigates the use of Quartz-Crystal Microbalance with Dissipation (QCM-D) technology, an analytical technique that measures physical parameters of thin-film structures, towards the successful monitoring of enzyme immobilisation strategies. These strategies are fundamental to the successful fabrication of biosensors, and the real-time monitoring of immobilised film formations is of considerable research interest. In the studies reported on in this Thesis, QCM-D technology was demonstrated to be an effective complementary technology in the prediction of film immobilisation techniques on the resultant biochemical kinetics of immobilised enzymes.
Subjects/Keywords: Laccase; Phenols; Biosensors
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APA (6th Edition):
Fogel, R. (2011). Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1007166
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fogel, Ronen. “Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors.” 2011. Thesis, Rhodes University. Accessed February 26, 2021.
http://hdl.handle.net/10962/d1007166.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fogel, Ronen. “Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors.” 2011. Web. 26 Feb 2021.
Vancouver:
Fogel R. Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors. [Internet] [Thesis]. Rhodes University; 2011. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10962/d1007166.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fogel R. Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors. [Thesis]. Rhodes University; 2011. Available from: http://hdl.handle.net/10962/d1007166
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Boston University
14.
Darlow, Ari.
Towards the development of a forensic DNA biosensor.
Degree: MS, Biomedical Forensic Sciences, 2015, Boston University
URL: http://hdl.handle.net/2144/13962
► In the forensic DNA field, quantitative PCR (qPCR) is commonly used to quantify the amount of deoxyribonucleic acid (DNA) in evidentiary samples. Though sensitive, this…
(more)
▼ In the forensic DNA field, quantitative PCR (qPCR) is commonly used to quantify the amount of deoxyribonucleic acid (DNA) in evidentiary samples. Though sensitive, this method is prone to error. Electrochemistry-based biosensors have been described as a possible alternative to qPCR. To this end, this work aims to develop a biosensor for forensic quantification by chemisorbing oligonucleotides functionalized to methylene blue onto the surface of gold screen-printed electrodes. Prior to this, the surface characteristics of the screen-printed gold electrode are examined through the use of a well-known redox probe Ru(NH3)62+/3+. Cyclic voltammetry (CV) and Square Wave voltammetry (SWV) were used to measure the current signal. The Randles-Sevčik equation was used to relate the area of the electrode with the current signal.
Surface examinations of the gold screen-printed electrodes suggested these electrodes are suitable for use as a forensic DNA biosensor. Attempts to bind the oligonucleotide to the gold electrode were conducted. Though binding was successful, the resultant SWV signal suggested methods to chemisorb DNA onto gold surfaces require optimization.
Subjects/Keywords: Chemistry; Biosensors; Forensic
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APA (6th Edition):
Darlow, A. (2015). Towards the development of a forensic DNA biosensor. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/13962
Chicago Manual of Style (16th Edition):
Darlow, Ari. “Towards the development of a forensic DNA biosensor.” 2015. Masters Thesis, Boston University. Accessed February 26, 2021.
http://hdl.handle.net/2144/13962.
MLA Handbook (7th Edition):
Darlow, Ari. “Towards the development of a forensic DNA biosensor.” 2015. Web. 26 Feb 2021.
Vancouver:
Darlow A. Towards the development of a forensic DNA biosensor. [Internet] [Masters thesis]. Boston University; 2015. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/2144/13962.
Council of Science Editors:
Darlow A. Towards the development of a forensic DNA biosensor. [Masters Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/13962
Hong Kong University of Science and Technology
15.
Zhu, Hongjie.
Intra-body communication channel characterization and modeling.
Degree: 2011, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-7925
;
https://doi.org/10.14711/thesis-b1155530
;
http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html
► To fully realize the potentials of the sensors in a personal healthcare system, body area network (BAN) is developed. Existing wireless sensor network technologies are…
(more)
▼ To fully realize the potentials of the sensors in a personal healthcare system, body area network (BAN) is developed. Existing wireless sensor network technologies are not suitable for BAN because they are not designed to minimize the power of the sensors and they do not scale well for a heterogeneous network. Intra-body communication (IBC), which uses the human body as the communication media, is a promising physical layer implementation for BAN in personal healthcare systems. It provides low-power, high-speed and reliable communication links. According to the signal transmission mechanism, IBC can be categorized into EM IBC and EF IBC. EM IBC has low power efficiency and limited bandwidth. The EF IBC, composited by a forward body path and a parasitic return path, has high power efficiency and large bandwidth. In this thesis, EF IBC channels are characterized and modeled. A critical issue in EF IBC is the separation of TX and RX grounds, because in most applications the TX and RX are battery-powered. Three characterizations setups are developed: 1) VNA with baluns; 2) TX board and SA; and 3) TX and RX board. S21 measured by the VNA with baluns setup shows a band-pass profile while the channel gain measured by the latter two setups shows a high-pass profile. Body channel radiation and body captured interference are also measured with the TX and RX board setup. Discussions of the results are provided FEM models of the first two characterization setups are established. Simulations reveal that the parasitic return path of the IBC channel is complicated. Simulation results using the FEM model of the TX and SA setup fit the measurement results well. Conclusions and future work are presented at the end of the thesis.
Subjects/Keywords: Medical electronics
; Biosensors
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APA (6th Edition):
Zhu, H. (2011). Intra-body communication channel characterization and modeling. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zhu, Hongjie. “Intra-body communication channel characterization and modeling.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed February 26, 2021.
http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zhu, Hongjie. “Intra-body communication channel characterization and modeling.” 2011. Web. 26 Feb 2021.
Vancouver:
Zhu H. Intra-body communication channel characterization and modeling. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Feb 26].
Available from: http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zhu H. Intra-body communication channel characterization and modeling. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
16.
Bergman, Jenny.
Development of Electrochemical Biosensors for Neurochemical Applications.
Degree: 2018, University of Gothenburg / Göteborgs Universitet
URL: http://hdl.handle.net/2077/54578
► The brain consists of billions of cells, including nerve cells, which have the ability of transforming an incoming electrical signal in to a chemical output…
(more)
▼ The brain consists of billions of cells, including nerve cells, which have the ability of transforming an incoming electrical signal in to a chemical output by the release of neurotransmitters through a process called exocytosis. Malfunction in neuronal communication has been linked to several conditions including Parkinson’s disease, schizophrenia, ADHD and autism why a better understanding of neuronal communication is of great importance contributing to increased knowledge about these conditions. For studying neuronal activity with single exocytosis events that occur on sub-millisecond to milliseconds time scale, analytical methods with high temporal resolution is the key. In my research, I have focused on developing miniaturized enzyme-based electrochemical biosensors for the detection of glucose and the neurotransmitters acetylcholine and glutamate. A biosensor is a sensor combining a biological component, here an enzyme, with a transducer part, here an electrode. In this thesis, biosensors based on a carbon fiber microelectrode modified with gold nanoparticles and enzyme have been developed with the aim to improve the temporal resolution of these probes compared to existing technology. By limiting the biosensor surface enzyme coverage close to a monolayer, millisecond time resolution was obtained. With this approach of biosensor design, we were able to detect vesicular release of acetylcholine from an artificial cell mimicking exocytosis as described in paper I, and glutamate release from mouse brain slice which is shown in paper IV. Also, a glucose biosensor able of co-detecting glucose and dopamine with millisecond time resolution has been fabricated as described in paper III. In paper II an analytical method for characterizing the interaction of the enzyme-gold nanoparticle interface was developed.
Subjects/Keywords: biosensors; electrochemistry; neurochemistry
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APA (6th Edition):
Bergman, J. (2018). Development of Electrochemical Biosensors for Neurochemical Applications. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/54578
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bergman, Jenny. “Development of Electrochemical Biosensors for Neurochemical Applications.” 2018. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed February 26, 2021.
http://hdl.handle.net/2077/54578.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bergman, Jenny. “Development of Electrochemical Biosensors for Neurochemical Applications.” 2018. Web. 26 Feb 2021.
Vancouver:
Bergman J. Development of Electrochemical Biosensors for Neurochemical Applications. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2018. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/2077/54578.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bergman J. Development of Electrochemical Biosensors for Neurochemical Applications. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2018. Available from: http://hdl.handle.net/2077/54578
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of the Western Cape
17.
Silwana, Bongiwe.
Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor
.
Degree: 2012, University of the Western Cape
URL: http://hdl.handle.net/11394/4619
► Environmental pollution is always the hottest topic in public conversation and one of the most concerned aspects of human health. The thin film sputtered microelectrode…
(more)
▼ Environmental pollution is always the hottest topic in public conversation and one of the most concerned aspects of human health. The thin film sputtered microelectrode devices have been developed to improve the quality of human health, by offering better monitoring capabilities. This thesis is divided into three parts and the studies were performed on chemical sensor technology currently available and under development using modified methods. In the first part of this thesis: (i) the studies are related to synthesis, characterization and polymerisation of polyaniline (PANI) and polyaniline-co-poly(2,2´-dithiodianline) (PANI-co-PDTDA). Polyaniline (PANI) and the copolymer of aniline with dithiodianiline, an aniline derivative containing S-S-links were of interest in polymer synthesis. Electrochemical synthesis was carried out in 1 M HCl and different concentrations of H2SO4 (1, 2.5, and 5 M) solutions for PANI and PANI-co-PDTDA respectively. The PANI and PANI-co-PDTDA were grown electrochemically on the surface of a glassy carbon electrode (GCE) by repetitive cyclic voltammetric scanning. Cyclic voltammetry (CV) was used to evaluate the differences between the electrochemical characteristics associated with growth of the copolymer and homopolymer, polyaniline (PANI). The surface concentration of PANI was estimated to be 2.64 × 10-1 mol.cm-2 while the film thickness was estimated to be 7.09 × 10-10cm and 1.49 × 10-9cm for scan rate and aquare root scan rate. In contrast, PANI-co-PDTDA concentrations (1, 2, 5 and 5 M H2SO4 solutions) gained a surface concentration (G) falling in the range 6.1 x 10-2 - 7.9 x 102 mol.cm-2 and a film thickness in the range 8.16 x 10 -9- 2.05x10-8cm. The second section of this thesis focused on the development of two sensors, Pt/PANI/HRP and Pt/PANI-co-PDTDA/HRP
biosensors. The biosensor described in this chapter focus on the use of horseradish peroxidise (HRP) with hydrogen peroxide as substrate, was constructed with the aim of further investigation of inhibition by heavy metals (Cd2+, Pb2+ and Hg2+). To achieve this, the enzyme HRP as the catalytic bio-element, was immobilised on the surface of a platinum electrode with PANI as a mediator. Immobilisation of HRP in conducting polymer matrices of PANI and PANI-co-PDTDA were achieved by electrochemical polymerisation. The use of amperometric detection allowed for the coupling of the biosensor with a portable potentiostat system (PalmSens). Differential pulse voltammetry (DPV) as technique was used as a detection method for inhibition determination. Selection of suitable pH values for biosensor performance was evaluated and the system showed optimal performance at pH 6.8 and 7.2 for Pt/PANI/HRP and Pt/PANIco- PDTDA/HRP
biosensors, respectively. The
biosensors developed in this work showed detection limits (LODs) of 0.32 mM and 0.0483 mM for PANI/HRP and PANI-co- PDTDA/HRP, respectively. For the Pt/PANI/HRP biosensor, the apparent Michaelis-Menten constant (Km app) value and maximum current (Imax) were evaluated from Lineweaver-Burk plots at…
Advisors/Committee Members: Somerset, V.S (advisor), Iwuoha, Emmanuel (advisor).
Subjects/Keywords: Biosensors;
Polyaniline;
Nanoparticles
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MLA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Silwana, B. (2012). Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor
. (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4619
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Silwana, Bongiwe. “Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor
.” 2012. Thesis, University of the Western Cape. Accessed February 26, 2021.
http://hdl.handle.net/11394/4619.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Silwana, Bongiwe. “Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor
.” 2012. Web. 26 Feb 2021.
Vancouver:
Silwana B. Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor
. [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/11394/4619.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Silwana B. Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor
. [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/4619
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
Barnett, Lauren Marie.
If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.
Degree: PhD, College of Letters & Science, 2017, Montana State University
URL: https://scholarworks.montana.edu/xmlui/handle/1/12752
► To see the activity of large, integrated neural circuits functioning in real-time inside of a living brain, neuroscientists will need multiple genetically-encoded fluorescent activity sensors…
(more)
▼ To see the activity of large, integrated neural circuits functioning in real-time inside of a living brain, neuroscientists will need multiple genetically-encoded fluorescent activity sensors that can be individually targeted to specific cell types, are fast enough to resolve multiple action potentials, can be distinguished from one another and imaged deep within the brain. The goal of this work is to better understand and improve upon the most recent generations of genetically-encoded Ca 2+ and voltage sensors, and to expand biosensor utility in two-photon excitation, which will be necessary to image neural activity deep within the brain. Genetically-encoded Ca 2+ sensors measure the intracellular Ca 2+ release that occurs downstream of an action potential. The GCaMP6 series are the best Ca 2+ sensors available, however little is known about how they work. Measurements of four different states in GCaMP6m reveal that its large Ca 2+-dependent change in 470 nm excited fluorescence is due to a redistribution of the chromophore protonation state, from a neutral form excited at ~400 nm to an anionic form excited at ~470 nm, via a change in pK a. Making pK a-altering mutations in GCaMP6m changes the Ca 2+-dependent fluorescence response. This highlights the importance of Delta pK a and identifies key amino acid positions that will be important for improving GCaMP6m and GCaMP-like
biosensors. A direct readout of an action potential would be ideal for capturing complex signal transduction in the brain. This will require a bright, fast voltage sensor. ElectricPk is the first genetically-encoded voltage sensor with a fluorescence response fast enough to resolve multiple action potentials in mammalian neurons. This design indicates it is possible to couple a fluorescence change with a very fast (~1 ms) voltage-dependent movement in the Ciona intestinalis voltage-sensitive phosphatase protein. Whether imaging a downstream Ca 2+ signal or a direct change in membrane potential, to image neuronal activity in deep brain tissue
biosensors will need to be brightly fluorescent in two-photon excitation. The two-photon directed evolution of green fluorescent proteins presented here is a proof-of-principle design that shows a high-throughput screen focused on improving the two-photon properties of a fluorescent protein is possible.
Advisors/Committee Members: Chairperson, Graduate Committee: Thomas Hughes (advisor), Thomas E. Hughes and Mikhail Drobizhev were co-authors of the article, 'Deciphering the molecular mechanism responsible for GCAMP6M's Ca 2+ dependent change in fluorescence' in the journal 'PLoSONE' which is contained within this thesis. (other), Mikhail Drobizhev and Thomas E. Hughes were co-authors of the article, 'Making pKa-altering mutations in GCAMP6M changes the Ca 2+-dependent fluorescence response' submitted to the journal 'PLoSONE' which is contained within this thesis. (other), Jelena Platisa, Marko Popovic, Vincent A. Pieribone and Thomas Hughes were co-authors of the article, 'A fluorescent, genetically-encoded voltage probe capable of resolving action potentials' in the journal 'PLoSONE' which is contained within this thesis. (other), Lauren M. Barnett, Mikhail Drobizhev, Geoffrey Wicks, Alexander Mikhaylov, Thomas E. Hughes and Aleksander Rebane were co-authors of the article, 'Two-photon directed evolution of green fluorescent proteins' in the journal 'Nature Scientific Reports' which is contained within this thesis. (other).
Subjects/Keywords: Biosensors.; Brain.; Fluorescence.
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to Zotero / EndNote / Reference
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APA (6th Edition):
Barnett, L. M. (2017). If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/12752
Chicago Manual of Style (16th Edition):
Barnett, Lauren Marie. “If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.” 2017. Doctoral Dissertation, Montana State University. Accessed February 26, 2021.
https://scholarworks.montana.edu/xmlui/handle/1/12752.
MLA Handbook (7th Edition):
Barnett, Lauren Marie. “If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.” 2017. Web. 26 Feb 2021.
Vancouver:
Barnett LM. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. [Internet] [Doctoral dissertation]. Montana State University; 2017. [cited 2021 Feb 26].
Available from: https://scholarworks.montana.edu/xmlui/handle/1/12752.
Council of Science Editors:
Barnett LM. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. [Doctoral Dissertation]. Montana State University; 2017. Available from: https://scholarworks.montana.edu/xmlui/handle/1/12752
University of Arizona
19.
Cho, Soohee.
Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms
.
Degree: 2017, University of Arizona
URL: http://hdl.handle.net/10150/625604
► Diagnostic biosensors are on the rise in the global market due to the increasing prevalence of diseases. Specifically, the point-of-care segment has made great strides…
(more)
▼ Diagnostic
biosensors are on the rise in the global market due to the increasing prevalence of diseases. Specifically, the point-of-care segment has made great strides due to the improvement of
biosensors' user-friendliness, simplicity, and clinical capabilities in the comfort of one's home. Although there are conventional diagnostic techniques, they are mutually time-consuming, costly, and labor-intensive. Not to mention, they are primarily dependent on bench-top or large immovable equipment. The widespread availability of smartphones has potentiated optical
biosensors towards delivery of rapid and point-of-care diagnostic
biosensors. Due to the affordability and user-friendliness of smartphones, smartphone-based
biosensors may become ubiquitously available. Additionally, microfluidic platforms possess small footprints and portability towards development of true point-of-care and real-time diagnostic
biosensors. In this dissertation, development of multiple diagnostic
biosensors on microfluidic platforms is discussed. Diagnostic
biosensors equipped with a smartphone-based optical detection show great promise of bringing clinical and bench-top laboratory capabilities for the convenience of the user, with reduced time, costs, and labor requirements. The widespread availability of point-of-care and real-time diagnostic
biosensors may show promise in securing global health.
Advisors/Committee Members: Yoon, Jeong-Yeol (advisor), Yoon, Jeong-Yeol (committeemember), Slack, Donald (committeemember), Uhlmann, Donald (committeemember), An, Lingling (committeemember).
Subjects/Keywords: Biosensors;
Microfluidics;
Smartphone
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cho, S. (2017). Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/625604
Chicago Manual of Style (16th Edition):
Cho, Soohee. “Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms
.” 2017. Doctoral Dissertation, University of Arizona. Accessed February 26, 2021.
http://hdl.handle.net/10150/625604.
MLA Handbook (7th Edition):
Cho, Soohee. “Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms
.” 2017. Web. 26 Feb 2021.
Vancouver:
Cho S. Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms
. [Internet] [Doctoral dissertation]. University of Arizona; 2017. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10150/625604.
Council of Science Editors:
Cho S. Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms
. [Doctoral Dissertation]. University of Arizona; 2017. Available from: http://hdl.handle.net/10150/625604
Queens University
20.
Swyer, Ian.
Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
.
Degree: Chemical Engineering, 2011, Queens University
URL: http://hdl.handle.net/1974/6528
► The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the…
(more)
▼ The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the array could be detected impedimetrically. The microelectrode array (denoted as DEP chip) uses dielectrophoretic forces to concentrate particles at its center. Initial results showed that there was a large electrode-silicon-electrode (ESE) capacitance which dominated at high frequencies. This capacitance was reduced by decreasing the electrode area and increasing the insulating layer thickness. These measures however proved fruitless as this capacitance was still significantly greater then the dielectric capacitance of the chip. This ESE capacitance can be eliminated through the use of a glass substrate so that the dielectric response of the chip dominates at higher frequencies. Since the ESE capacitance prevented experimental validation of impedance spectroscopy as a signal transduction method, computer simulations were performed. These simulations indicated that capture with the current DEP chips would not have a significant impact on the impedance of the chip. Decreasing the electrode gap distance and reducing the area of the electrodes, which is recommended for future work, can remedy this.
As measureable changes in the dielectric capacitance of the chip are not possible, a reaction scheme was developed to translate the capture of viral particles into a change in medium conductivity. An ELISA type system was proposed where the viral particles would be functionalized with urease. This uease would then be used to degrade non-ionic urea into ionic products thereby increasing the medium conductivity. A model was formulated to predict the conductivity increase expected for low concentrations, and validated using higher concentrations of biotinylated-urease. Urease from commercial sources proved not to be a viable option as it does not possess a high enough activity to produce a significant conductivity change given the low concentrations of viral particles expected after collection. Urease with suitable activity is produced by the organism Ureaplasma urealyticum which has an activity of 180 000 µmol urea catalyzed min-1 mg urease-1. It is not recommended that this method be pursued further due to technical challenges that would be encountered.
Subjects/Keywords: Impedance
;
Biosensors
;
Microfluidic
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Manager
APA (6th Edition):
Swyer, I. (2011). Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
. (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
.” 2011. Thesis, Queens University. Accessed February 26, 2021.
http://hdl.handle.net/1974/6528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
.” 2011. Web. 26 Feb 2021.
Vancouver:
Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
. [Internet] [Thesis]. Queens University; 2011. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/1974/6528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays
. [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Oklahoma
21.
Godman, Nicholas.
Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes.
Degree: PhD, 2015, University of Oklahoma
URL: http://hdl.handle.net/11244/15464
► New methods of constructing enzymatic bioelectrodes based on ferrocene-modified linear poly(ethylenimine) (LPEI) were explored with the intent of lowering device fabrication times. Redox polymers were…
(more)
▼ New methods of constructing enzymatic bioelectrodes based on ferrocene-modified linear poly(ethylenimine) (LPEI) were explored with the intent of lowering device fabrication times. Redox polymers were synthesized and characterized for usage as both anode and cathode materials.
Photolithography was used to form patterned films based on ferrocenylpropyl-modified linear poly(ethylenimine-co-allylethylenimine) (Fc-C3-LPAEI). Fc-C3-LPAEI (50% allylated, 5% ferrocene) films were crosslinked in the presence of glucose oxidase (GOX) using both photogenerated radicals and nitrenes. Biosensor efficiency was found to be a function of both polymer connectivity and enzyme stability. Fc-C3-LPAEI/GOX bioanodes were capable of generating 44.9 ± 1.3 µAcm-2 after five hours irradiation using a photogenerated dinitrene from 1,2-bis(2-azidoethoxy)ethane.
Both electrostatic and covalent layer-by-layer assembly were used for the fabrication of polymer/enzyme composite thin films. Ferrocenylhexyl- and ferrocenylpropyl- modified LPEI (Fc-C6-LPEI, Fc-C3-LPEI. 17-20% ferrocene) were used with periodate modified glucose oxidase (p-GOX) for the construction of enzymatic bioanodes capable of generating up to 381 ± 3 and 1417 ± 63 µAcm-2, respectively. Fc-C3-LPEI/p-GOX biofuel cells generated 86 ± 3 µWcm-2 at pH 7 when poised against an air-breathing Pt cathode. A chloroferrocene-modifed redox polymer (FcCl-C3-LPEI, 17-20% chloroferrocene) was assembled with laccase to construct biocathodes capable of generating up to 5.75 ± 0.14 µAcm-2 on planar gold electrodes and 32.3 ± 3.2 µAcm-2 on nitric acid oxidized carbon paper.
Lastly, sulfur and paracyclophane derivatives were copolymerized via inverse vulcanization to afford materials with the possibility of being incorporated in lithium-sulfur batteries. Differential scanning calorimetry was used to monitor the exothermic polymerization between the reactants, and the reaction parameters were optimized by varying the ratios of the starting materials.
Advisors/Committee Members: Glatzhofer, Daniel (advisor), Nicholas, Kenneth (committee member), Yip, Wai Tak (committee member), Thomson, Robert (committee member), Crossley, Steven (committee member).
Subjects/Keywords: Biosensors; Electrochemistry; Polymers
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Manager
APA (6th Edition):
Godman, N. (2015). Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/15464
Chicago Manual of Style (16th Edition):
Godman, Nicholas. “Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes.” 2015. Doctoral Dissertation, University of Oklahoma. Accessed February 26, 2021.
http://hdl.handle.net/11244/15464.
MLA Handbook (7th Edition):
Godman, Nicholas. “Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes.” 2015. Web. 26 Feb 2021.
Vancouver:
Godman N. Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes. [Internet] [Doctoral dissertation]. University of Oklahoma; 2015. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/11244/15464.
Council of Science Editors:
Godman N. Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes. [Doctoral Dissertation]. University of Oklahoma; 2015. Available from: http://hdl.handle.net/11244/15464
Rutgers University
22.
Zhen, Huajun, 1985-.
Investigation of bioaerosol characterization bias due to aerosolization and sampling.
Degree: PhD, Environmental Sciences, 2015, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/47620/
► Bioaerosols have been investigated for their adverse effects on human health and also their roles in cloud formation, precipitation and atmospheric chemical reactions. In these…
(more)
▼ Bioaerosols have been investigated for their adverse effects on human health and also their roles in cloud formation, precipitation and atmospheric chemical reactions. In these studies, it is often necessary to collect biological cells from the airborne state or to disperse the cells into the air using a variety of sampling and aerosolization devices. These devices inevitably exert stress on the cells and result in changes in their biological characteristics, e.g. loss of culturability, impairment of cell membrane, and change in cellular activities. It is highly likely that after experiencing such stress, collected microorganisms are not representative of cells prior to sampling and aerosolization, and thus could introduce biases in their characterization. In this dissertation, the potential bioaerosol characterization biases induced by several commonly used sampling and aerosolization devices were investigated. The stress experienced by E. coli during aerosolization was found to depend on a particular aerosolization device. Particularly, a newly developed pneumatic nebulizer, the Single-Pass Aerosolizer, was shown to better preserve cell culturability and membrane integrity compared to the commonly used Collison nebulizer at similar biological particles output concentrations. Several bioaerosol samplers that employ filtration, impingement, impaction and electrostatic precipitation for sample collection were tested with respect to their effects on the cell membrane integrity and cellular 16S rRNA content of Escherichia coli cells. Sampling stress resulted in severe membrane impairment to E. coli aerosols, leading to the release of genomic DNA as extracellular molecules. Extracellular DNA should be taken into account when analyzing bioaerosol samples to more accurately quantify bacterial presence. Cell membrane damage to bioaerosols depended on which sampler was used and could be reduced by modifying specific operational parameters. E. coli cells exhibited variation in 16S ribosomal RNA (rRNA) level when exposed to long-term air sampling in laboratory experiment, suggesting a change of biological activity in response to sampling stress. The importance of this effect for those taxa in airborne bacterial community from a variety of environments should be examined. The abundance of bacterial 16S rRNA in bioaerosols collected from an outdoor environment was about two orders of magnitude higher than that of 16S rRNA gene. However, the sampler-dependent bias effect on analysis of 16S rRNA sequences for bacterial community composition was minimal in investigated outdoor bioaerosols.
Advisors/Committee Members: Mainelis, Gediminas (chair), Fennell, Donna E (internal member), Reinfelder, John (internal member), SCHWANDER, STEPHAN (outside member).
Subjects/Keywords: Biosensors; Aerosols – Measurement
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APA (6th Edition):
Zhen, Huajun, 1. (2015). Investigation of bioaerosol characterization bias due to aerosolization and sampling. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/47620/
Chicago Manual of Style (16th Edition):
Zhen, Huajun, 1985-. “Investigation of bioaerosol characterization bias due to aerosolization and sampling.” 2015. Doctoral Dissertation, Rutgers University. Accessed February 26, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/47620/.
MLA Handbook (7th Edition):
Zhen, Huajun, 1985-. “Investigation of bioaerosol characterization bias due to aerosolization and sampling.” 2015. Web. 26 Feb 2021.
Vancouver:
Zhen, Huajun 1. Investigation of bioaerosol characterization bias due to aerosolization and sampling. [Internet] [Doctoral dissertation]. Rutgers University; 2015. [cited 2021 Feb 26].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/47620/.
Council of Science Editors:
Zhen, Huajun 1. Investigation of bioaerosol characterization bias due to aerosolization and sampling. [Doctoral Dissertation]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/47620/
University of Alberta
23.
Ko, Wooree.
Integration of Nanomechanical Resonators in Microfluidic
Systems for Specific Protein Detection.
Degree: MS, Department of Electrical and Computer
Engineering, 2009, University of Alberta
URL: https://era.library.ualberta.ca/files/vx021f677
► Nanomechanical resonators offer a pathway towards highly sensitive and label-free detection of biomolecules by transducing the mass of bound analytes into resonant frequency shifts. Zeptogram…
(more)
▼ Nanomechanical resonators offer a pathway towards
highly sensitive and label-free detection of biomolecules by
transducing the mass of bound analytes into resonant frequency
shifts. Zeptogram level and even single cell detections have been
successfully achieved by others. However, these experiments have
been performed through traditional âdip-and-dryâ method,
requiring the use of a large quantity of analyte. Such a system is
not readily amenable to automation, is subject to contamination and
is prone to inter-run variation. Integration of nanomechanical
resonators into a microfluidic system would facilitate sample
delivery and provide a stable Lab-On-a-Chip (LOC) system that could
be effectively used for diagnostic tests. There have been a few
demonstrations of integrating nanomechanical resonators with
microfuidic systems. However, these works involved external syringe
pumps and valves for sample delivery which make their system bulky,
complex and not suitable for point-of-care testing applications.
Here we have developed a nanomechanical resonator-based
microfluidic system that delivers sample using on-chip automated
pump and valve systems and that demonstrates the biosensing
capabilities with the limit of detection in femtogram range using
minute amount of sample. To our best of knowledge, this is the
first integration of nanoresonator-based sensing platform with the
automated on-chip sample delivery system. Such integration can
provide the system with simplicity, portability and potential
realization as easily accessible point-of-care diagnostic
tools.
Subjects/Keywords: Nanoelectromechanical systems; Resonators; Biosensors
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Export
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Manager
APA (6th Edition):
Ko, W. (2009). Integration of Nanomechanical Resonators in Microfluidic
Systems for Specific Protein Detection. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/vx021f677
Chicago Manual of Style (16th Edition):
Ko, Wooree. “Integration of Nanomechanical Resonators in Microfluidic
Systems for Specific Protein Detection.” 2009. Masters Thesis, University of Alberta. Accessed February 26, 2021.
https://era.library.ualberta.ca/files/vx021f677.
MLA Handbook (7th Edition):
Ko, Wooree. “Integration of Nanomechanical Resonators in Microfluidic
Systems for Specific Protein Detection.” 2009. Web. 26 Feb 2021.
Vancouver:
Ko W. Integration of Nanomechanical Resonators in Microfluidic
Systems for Specific Protein Detection. [Internet] [Masters thesis]. University of Alberta; 2009. [cited 2021 Feb 26].
Available from: https://era.library.ualberta.ca/files/vx021f677.
Council of Science Editors:
Ko W. Integration of Nanomechanical Resonators in Microfluidic
Systems for Specific Protein Detection. [Masters Thesis]. University of Alberta; 2009. Available from: https://era.library.ualberta.ca/files/vx021f677
24.
Ζάβαλη, Μαρία-Δημητρούλα.
Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.
Degree: 2009, University of Patras
URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124
► Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε…
(more)
▼ Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε αυτή την αναφορά, παρουσιάζεται μια μεθοδολογία η οποία βασίζεται στη Φασματοσκοπία Ανάκλασης Λευκού Φωτός και ενδείκνυται για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αντιδράσεων που λαμβάνουν χώρα σε στερεά υποστρώματα. Η οπτική διάταξη αποτελείται από μια VIS–NIR οπτική πηγή η οποία επικοινωνεί μέσω οπτικής ίνας με φασματοφωτόμετρο συνδεδεμένο σε Η/Υ. Το εξωτερικό τμήμα της οπτικής ίνας κατευθύνει το φως κάθετα πάνω στην επιφάνεια όπου συμβαίνουν οι αλληλεπιδράσεις μεταξύ των βιιομορίων. Το ανακλώμενο φως συγκεντρώνεται από το εσωτερικό τμήμα της οπτικής ίνας και κατευθύνεται στο φασματοφωτόμετρο. Οι αντιδράσεις λαμβάνουν χώρα σε επιφάνεια διοξειδίου του πυριτίου επικαλυμμένη με πολυμερικό υμένιο. Μια αντλία χρησιμοποιείται για την παροχή των αντιδραστηρίων με ελεγχόμενο ρυθμό σε ένα μικρορρευστομηχανικό κανάλι. Το φάσμα της ανάκλασης καταγράφεται σε πραγματικό χρόνο. Οι αντιδράσεις μεταξύ των πρωτεϊνικών μορίων γίνονται αντιληπτές σαν μετατοπίσεις του μήκους κύματος εκεί που παρατηρείται το φαινόμενο της ενισχυτικής συμβολής. Οι μετατοπίσεις αυτές στο μήκος κύματος συμβαίνουν λόγω του σχηματισμού πρωτεϊνικού στρώματος είτε κατά τη διάρκεια της προσρόφησης των αντισωμάτων στο στερεό υπόστρωμα ή λόγω αλλαγής στο πάχος του πρωτεϊνικού στρώματος όταν τα συμπληρωματικά αντιγόνα δεσμεύονται από τα ήδη ακινητοποιημένα αντισώματα. Η προτεινόμενη μεθοδολογία εφαρμόστηκε για την παρακολούθηση σε πραγματικό χρόνο, χωρίς τη χρήση ιχνηθέτη της αντίδρασης μεταξύ των συμπληρωματικών μορίων βιοτίνης-στρεπταβιδίνης όπως επίσης και για την ανίχνευση της πρόσδεσης των αντιγόνων mouse IgG από ήδη ακινητοποιημένα αντισώματα anti-mouse IgG. Mouse IgG σε συγκεντρώσεις μικρότερες των 150 pM ανιχνεύτηκαν σε πολύ μικρούς χρόνους αντίδρασης (10 min). Ο οπτικός αισθητήρας είναι μια απλή, γρήγορη, χαμηλού κόστους διάταξη για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων που τον καθιστά κατάλληλο για διάφορες αναλυτικές εφαρμογές.
Label-free monitoring of biomolecular reactions in real-time is of great interest since it can provide direct results concerning binding kinetics and equilibrium constants. In this report, a method based on White Light Reflectance Spectroscopy (WLRS) is presented that is capable for real-time monitoring of biomolecular reactions taking place on a solid surface. The optical setup consists of a VIS–NIR light source connected through a bifurcated optical fiber to a PC-driven spectrophotometer. The outer part of the optical fibre guides the light vertically onto the surface where the biomolecular reactions occur. The reflected light is collected from the central part of the optical fibre and is directed to the spectrophotometer. The reactions take place on the top of a polymer covered silicon dioxide surface. A microfluidic module in combination with a micropump is used to supply the…
Advisors/Committee Members: Κουτσούρης, Δημήτριος, Zavali, Maria-Dimitroula, Κουτσούρης, Δημήτριος, Νικήτα, Κωνσταντίνα, Μισιακός, Κωνστατίνος.
Subjects/Keywords: Βιοαισθητήρες; Αντισώματα; 610.28; Biosensors; Antibodies
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APA (6th Edition):
Ζάβαλη, . (2009). Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3124
Chicago Manual of Style (16th Edition):
Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Masters Thesis, University of Patras. Accessed February 26, 2021.
http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.
MLA Handbook (7th Edition):
Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Web. 26 Feb 2021.
Vancouver:
Ζάβαλη . Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Internet] [Masters thesis]. University of Patras; 2009. [cited 2021 Feb 26].
Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.
Council of Science Editors:
Ζάβαλη . Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Masters Thesis]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124
Stellenbosch University
25.
Lloyd, Alexander M.
Quantification of CD4+ cell count via a nanofibre-based biosensor.
Degree: MEng, Electrical and Electronic Engineering, 2019, Stellenbosch University
URL: http://hdl.handle.net/10019.1/106110
► ENGLISH ABSTRACT: Antigen-substrate binding has been found to cause a change in the resistance of conductive nanofibre substrates. This thesis endeavoured to exploit this phenomenon…
(more)
▼ ENGLISH ABSTRACT: Antigen-substrate binding has been found to cause a change in the resistance of conductive
nanofibre substrates. This thesis endeavoured to exploit this phenomenon to produce
sensors capable of quantifying CD4+ cell count. Producing nanofibre substrates with appropriate
robustness and reproducibility cheaply remains challenging. Previous methods
coated non-conductive nanofibres with a non-uniform conductive coating or used carbon
nanofibre mats that were very susceptible to mechanical stress. To address these factors
that negatively affect reproducibility, intrinsically conductive nanofibres were electrospun
from an organic semiconducting polymer through an adaptation of an existing method
that decreased the overall material cost. The production process was tuned so as to
produce mats with appropriate electrical characteristics and the fibres were aligned in a
uniform direction so as to provide directed current paths. These mats were then spun
directly onto interdigitated electrodes that had been printed onto semi-hydrophobic paper
with silver ink via a modified inkjet printer. By employing appropriate cross-linking
chemistry, antibodies were bound to the produced nanofibres. These antibodies served as
the biorecognition element for the sensor. The data were analysed and it was evident that
the binding event did indeed cause a change in resistance. While this resistance could not
be repeatably quantified owing to base variance between electrodes, this thesis lays the
groundwork for further research.
AFRIKAANSE OPSOMMING: Daar is al bevind dat antigeen-substraat binding ’n verandering veroorsaak in die weerstand
van geleidende nanovesel-substrate. Hierdie tesis het gepoog om hierdie verskynsel
in te span met die oog op die vervaardiging van sensors wat in staat is om CD4+ seltelling
te kwantifiseer. Om nanovesel-substrate met ’n gepaste vlak van robuustheid en
reproduseerbaarheid op ’n goedkoop wyse te produseer, bly ’n uitdaging. Vorige metodes
het behels dat nie-geleibare nanovesels bedek word met ’n nie-uniforme geleidende laag,
of het gebruik gemaak van koolstof nanovesel matte wat baie vatbaar was vir meganiese
stres. Dit was noodsaaklik om hierdie faktore aan te spreek weens hul negatiewe impak
op reproduseerbaarheid. Gevolglik is inherent geleidende nanovesels ge-elektrospin uit ’n
organiese semi-geleidende polimeer; dit is vermag deur ’n bestaande metode aan te pas op
so ’n wyse dat die kostes verbonde daaraan verlaag is. Die proses is verfyn met die doel
om matte met die gepaste elektriese eienskappe te produseer en vesels wat in ’n uniforme
rigting le, te vervaardig; sodoende is direkte stroompaaie geskep. Hierdie nanoveselmatte
is gespin op elektrodes; die elektrodes is met silwer ink gedruk op semi-hidrofobiese papier
deur middel van ’n aangepaste injet drukker. Deur die gebruik van kruisbindingchemie
is teenliggaampies gebind tot die geproduseerde nanovesels. Hierdie teenliggaampies het
gedien as die ”biorecognitionëlement. Uit die analise van die data…
Advisors/Committee Members: Perold, W. J., Stellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering..
Subjects/Keywords: UCTD; Nanofibers; Biosensors; CD4 antigen
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lloyd, A. M. (2019). Quantification of CD4+ cell count via a nanofibre-based biosensor. (Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/106110
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lloyd, Alexander M. “Quantification of CD4+ cell count via a nanofibre-based biosensor.” 2019. Thesis, Stellenbosch University. Accessed February 26, 2021.
http://hdl.handle.net/10019.1/106110.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lloyd, Alexander M. “Quantification of CD4+ cell count via a nanofibre-based biosensor.” 2019. Web. 26 Feb 2021.
Vancouver:
Lloyd AM. Quantification of CD4+ cell count via a nanofibre-based biosensor. [Internet] [Thesis]. Stellenbosch University; 2019. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/10019.1/106110.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lloyd AM. Quantification of CD4+ cell count via a nanofibre-based biosensor. [Thesis]. Stellenbosch University; 2019. Available from: http://hdl.handle.net/10019.1/106110
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
McMaster University
26.
Ephraim, Lydia E.
Simultaneously Isolating Multiple Biosensors for Multiple Targets from a Single SELEX Procedure.
Degree: MSc, 2015, McMaster University
URL: http://hdl.handle.net/11375/17214
► SELEX is a selective amplification technique based on the assumption that functional nucleic acids (FNAs) can be found in a pool of chemically synthesized random…
(more)
▼ SELEX is a selective amplification technique based on the assumption that functional nucleic acids (FNAs) can be found in a pool of chemically synthesized random nucleic acid sequences. These FNAs can either bind to a target of interest (aptamers) or catalyze a chemical reaction (DNAzymes and ribozymes) or both (aptazyme). The aptazymes discussed herein, are RNA-cleaving DNAzymes that become catalytically active upon target recognition at the aptamer domain. In chapter 2, two SELEX experiments were performed in parallel to determine whether it was possible to obtain multiple aptazymes from a single SELEX procedure. Two different crude intercellular mixtures (CIM) each containing 35 unique overexpressed ASKA clone gene products were used. After 10 rounds of SELEX, the sequence pool was analyzed using Illumina Genome Analyzer. The sequences tested appeared to bind to a protein other than the 35 gene products of interest. To redirect these SELEX experiments towards the gene products of interest, 12 gene products from each parallel selection was purified and incubated with sequences from round 6 of the CIM SELEX. A total of 13 rounds of selection were performed using these purified proteins. The sequences were analyzed and found to either not cleave at all or self-cleave. In chapter 3 a similar SELEX approach was explored using 36 purified proteins, from the CIM SELEX to isolate protein-binding DNA aptamers. This SELEX approach served as another means to explore isolating multiple aptamers against multiple targets. However both the RNA-cleaving aptazyme and protein-binding DNA aptamer SELEX experiments both experienced challenges in non-specific binding. Although negative selection steps were taken in order to avoid non-specific binding species, such sequences were still isolated. In order for these approaches to be successful, negative selection steps are required to remove any self-cleaving and non-specific FNAs. Although these studies did not conclusively give rise to the desired FNAs, it has produced some insight to the potential setbacks associated with developing a screen for multiple targets.
Thesis
Master of Science (MSc)
Advisors/Committee Members: Li, Yingfu, Chemistry and Chemical Biology.
Subjects/Keywords: aptamers; aptazymes; biosensors; SELEX
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ephraim, L. E. (2015). Simultaneously Isolating Multiple Biosensors for Multiple Targets from a Single SELEX Procedure. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/17214
Chicago Manual of Style (16th Edition):
Ephraim, Lydia E. “Simultaneously Isolating Multiple Biosensors for Multiple Targets from a Single SELEX Procedure.” 2015. Masters Thesis, McMaster University. Accessed February 26, 2021.
http://hdl.handle.net/11375/17214.
MLA Handbook (7th Edition):
Ephraim, Lydia E. “Simultaneously Isolating Multiple Biosensors for Multiple Targets from a Single SELEX Procedure.” 2015. Web. 26 Feb 2021.
Vancouver:
Ephraim LE. Simultaneously Isolating Multiple Biosensors for Multiple Targets from a Single SELEX Procedure. [Internet] [Masters thesis]. McMaster University; 2015. [cited 2021 Feb 26].
Available from: http://hdl.handle.net/11375/17214.
Council of Science Editors:
Ephraim LE. Simultaneously Isolating Multiple Biosensors for Multiple Targets from a Single SELEX Procedure. [Masters Thesis]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/17214
Penn State University
27.
Narayanan, Rajaram.
Carbon nanotube flow sensors: A comprehensive study
.
Degree: 2011, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/11914
► It has been established that flow of fluids over Carbon Nanotubes (CNTs) generate voltages. The “Sood effect” as it is colloquially referred to, can spawn…
(more)
Subjects/Keywords: flow sensors; Carbon Nanotubes; biosensors
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Narayanan, R. (2011). Carbon nanotube flow sensors: A comprehensive study
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/11914
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Narayanan, Rajaram. “Carbon nanotube flow sensors: A comprehensive study
.” 2011. Thesis, Penn State University. Accessed February 26, 2021.
https://submit-etda.libraries.psu.edu/catalog/11914.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Narayanan, Rajaram. “Carbon nanotube flow sensors: A comprehensive study
.” 2011. Web. 26 Feb 2021.
Vancouver:
Narayanan R. Carbon nanotube flow sensors: A comprehensive study
. [Internet] [Thesis]. Penn State University; 2011. [cited 2021 Feb 26].
Available from: https://submit-etda.libraries.psu.edu/catalog/11914.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Narayanan R. Carbon nanotube flow sensors: A comprehensive study
. [Thesis]. Penn State University; 2011. Available from: https://submit-etda.libraries.psu.edu/catalog/11914
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
28.
Apon Numnuam.
Potentiometric and capacitive affinity biosensors
.
Degree: คณะวิทยาศาสตร์ ภาควิชาเคมี, 2008, Prince of Songkla University
URL: http://kb.psu.ac.th/psukb/handle/2010/7425
Subjects/Keywords: Electrochemistry;
Biosensors
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APA ·
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MLA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Numnuam, A. (2008). Potentiometric and capacitive affinity biosensors
. (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2010/7425
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Numnuam, Apon. “Potentiometric and capacitive affinity biosensors
.” 2008. Thesis, Prince of Songkla University. Accessed February 26, 2021.
http://kb.psu.ac.th/psukb/handle/2010/7425.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Numnuam, Apon. “Potentiometric and capacitive affinity biosensors
.” 2008. Web. 26 Feb 2021.
Vancouver:
Numnuam A. Potentiometric and capacitive affinity biosensors
. [Internet] [Thesis]. Prince of Songkla University; 2008. [cited 2021 Feb 26].
Available from: http://kb.psu.ac.th/psukb/handle/2010/7425.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Numnuam A. Potentiometric and capacitive affinity biosensors
. [Thesis]. Prince of Songkla University; 2008. Available from: http://kb.psu.ac.th/psukb/handle/2010/7425
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of California – Riverside
29.
Tran, Thien-Toan Huu.
Carbon Nanomaterial-Based Chemiresistive Biosensors for Detection of Secretory Protein Biomarkers of Citrus Greening Disease.
Degree: Bioengineering, 2018, University of California – Riverside
URL: http://www.escholarship.org/uc/item/7bj3x2qh
► Citrus greening disease, also known as Huanglongbing (HLB), is posing a worldwide threat to the multi-billion dollars citrus industry. Currently, there are no cures for…
(more)
▼ Citrus greening disease, also known as Huanglongbing (HLB), is posing a worldwide threat to the multi-billion dollars citrus industry. Currently, there are no cures for infected plants while containment of the spread of disease is heavily dependent on early detection of infected hosts for quarantine. The pathogen responsible for causing the disease is the bacteria Candidatus Liberibacter asiaticus (CLas). Thus, it is imperative that disease management strategies address current demands for accurate, timely, and robust disease detection and diagnosis minimize the spread of disease. By adopting a novel detection strategy targeting a recently discovered secreted protein biomarker, SDE1, which is unique to CLas, we hope to overcome the challenges faced by current detection methods, such as nucleic acid-based and symptom-based which have been found prone to false negatives and mis-diagnoses, respectively. To do this, we have worked to procure and characterize the antibodies specific for the novel HLB biomarker. In the process of characterizing the anti-SDE1 antibodies, we have also developed a specific and sensitive enzyme-linked immunosorbent assay (ELISA) for high-throughput and point-of-laboratory analysis of citrus plant samples. With the final goal of using the anti-SDE1 antibodies to develop sensitive, facile, and specific HLB detection method, we have integrated these antibodies into nanoscale electrical biosensor platforms. Using the novel semiconducting carbon nanomaterials, reduced graphene oxide (RGO) and single-walled carbon nanotubes (SWNTs), as the electrical transducer element for our biosensors, we have developed chemiresistive biosensors that demonstrate specificity and sensitivity to the SDE1 biomarker in simple phosphate buffer and in plant extracts.
Subjects/Keywords: Bioengineering; Biosensors; Carbon Nanomaterials; Immunoassays
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APA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Tran, T. H. (2018). Carbon Nanomaterial-Based Chemiresistive Biosensors for Detection of Secretory Protein Biomarkers of Citrus Greening Disease. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/7bj3x2qh
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tran, Thien-Toan Huu. “Carbon Nanomaterial-Based Chemiresistive Biosensors for Detection of Secretory Protein Biomarkers of Citrus Greening Disease.” 2018. Thesis, University of California – Riverside. Accessed February 26, 2021.
http://www.escholarship.org/uc/item/7bj3x2qh.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tran, Thien-Toan Huu. “Carbon Nanomaterial-Based Chemiresistive Biosensors for Detection of Secretory Protein Biomarkers of Citrus Greening Disease.” 2018. Web. 26 Feb 2021.
Vancouver:
Tran TH. Carbon Nanomaterial-Based Chemiresistive Biosensors for Detection of Secretory Protein Biomarkers of Citrus Greening Disease. [Internet] [Thesis]. University of California – Riverside; 2018. [cited 2021 Feb 26].
Available from: http://www.escholarship.org/uc/item/7bj3x2qh.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tran TH. Carbon Nanomaterial-Based Chemiresistive Biosensors for Detection of Secretory Protein Biomarkers of Citrus Greening Disease. [Thesis]. University of California – Riverside; 2018. Available from: http://www.escholarship.org/uc/item/7bj3x2qh
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Kosin Teeparuksapun.
Development of electrode for biosensor
.
Degree: คณะวิทยาศาสตร์ ภาควิชาเคมี, 2008, Prince of Songkla University
URL: http://kb.psu.ac.th/psukb/handle/2016/13002
Subjects/Keywords: Electrodes;
Biosensors
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Teeparuksapun, K. (2008). Development of electrode for biosensor
. (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2016/13002
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Teeparuksapun, Kosin. “Development of electrode for biosensor
.” 2008. Thesis, Prince of Songkla University. Accessed February 26, 2021.
http://kb.psu.ac.th/psukb/handle/2016/13002.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Teeparuksapun, Kosin. “Development of electrode for biosensor
.” 2008. Web. 26 Feb 2021.
Vancouver:
Teeparuksapun K. Development of electrode for biosensor
. [Internet] [Thesis]. Prince of Songkla University; 2008. [cited 2021 Feb 26].
Available from: http://kb.psu.ac.th/psukb/handle/2016/13002.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Teeparuksapun K. Development of electrode for biosensor
. [Thesis]. Prince of Songkla University; 2008. Available from: http://kb.psu.ac.th/psukb/handle/2016/13002
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
◁ [1] [2] [3] [4] [5] … [35] ▶
. 
Open Access Theses and Dissertations
