subject:(Biosensors)

Wake Forest University

1. Taylor, Alexander David. PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE.

Degree: 2016, Wake Forest University

URL: http://hdl.handle.net/10339/64180

► The basis of plasmonic sensors is the resonant coupling between the oscillations of free electrons, called plasmons, and incident visible light waves. By confining these… (more)

Subjects/Keywords: Biosensors

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APA (6^th Edition):

Taylor, A. D. (2016). PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/64180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Taylor, Alexander David. “PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE.” 2016. Thesis, Wake Forest University. Accessed February 23, 2020. http://hdl.handle.net/10339/64180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Taylor, Alexander David. “PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE.” 2016. Web. 23 Feb 2020.

Vancouver:

Taylor AD. PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE. [Internet] [Thesis]. Wake Forest University; 2016. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/10339/64180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Taylor AD. PLASMONIC NANOPARTICLES AS VERSATILE NANORULERS FOR SENSING APPLICATIONS: DEVELOPING THE NANOPARTICLE-ON-MIRROR ARCHITECTURE. [Thesis]. Wake Forest University; 2016. Available from: http://hdl.handle.net/10339/64180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

2. Chalishazar, Vishvas. Nanostructured Optical Devices for Biosensing Applications.

Degree: MS, Electrical and Computer Engineering, 2016, Oregon State University

URL: http://hdl.handle.net/1957/58140

► We demonstrated a sensitive biosensor based on plasmonic metal composite nanostructures. The device can be fabricated and duplicated with ease using a novel nanoprinting technology… (more)

Subjects/Keywords: Nanostructure; Biosensors

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APA (6^th Edition):

Chalishazar, V. (2016). Nanostructured Optical Devices for Biosensing Applications. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/58140

Chicago Manual of Style (16^th Edition):

Chalishazar, Vishvas. “Nanostructured Optical Devices for Biosensing Applications.” 2016. Masters Thesis, Oregon State University. Accessed February 23, 2020. http://hdl.handle.net/1957/58140.

MLA Handbook (7^th Edition):

Chalishazar, Vishvas. “Nanostructured Optical Devices for Biosensing Applications.” 2016. Web. 23 Feb 2020.

Vancouver:

Chalishazar V. Nanostructured Optical Devices for Biosensing Applications. [Internet] [Masters thesis]. Oregon State University; 2016. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1957/58140.

Council of Science Editors:

Chalishazar V. Nanostructured Optical Devices for Biosensing Applications. [Masters Thesis]. Oregon State University; 2016. Available from: http://hdl.handle.net/1957/58140

Oregon State University

3. Roussel, Nicolas. Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents.

Degree: MS, Electrical and Computer Engineering, 2003, Oregon State University

URL: http://hdl.handle.net/1957/30351

► Image segmentation is the process by which an image is divided into number of regions. The regions are to be homogeneous with respect to some… (more)

▼ Image segmentation is the process by which an image is divided into number of regions. The regions are to be homogeneous with respect to some property. Definition of homogeneity depends mainly on the expected patterns of the objects of interest. The algorithms designed to perform these tasks can be divided into two main families: Splitting Algorithms and Merging Algorithms. The latter comprises seeded region growing algorithms which provide the basis for our work. Seeded region growing methods such as Marker initiated Watershed segmentation depend principally on the quality and relevance of the initial seeds. In situations where the image contains a variety of aggregated objects of different shapes, finding reliable initial seeds can be a very complex task. This thesis describes a versatile approach for finding initial seeds on images featuring objects distinguishable by their structural and intensity profiles. This approach involves the use of hierarchical trees containing various information on the objects in the image. These trees can be searched for specific pattern to generate the initial seeds required to perform a reliable region growing process. Segmentation results are shown in this thesis. The above image segmentation scheme has been applied to detect isolated living cells in a sequence of frames and monitor their behavior through the time. The tissues utilized for these studies are isolated from the scales of Betta Splendens fish family. Since the isolated cells or chromatophores are sensitive to various kinds of toxic agents, a creation of cell-based toxin detector was suggested. Such sensor operation depends on an efficient segmentation of cell images and extraction of pertinent visual features. Our ultimate objective is to model and classify the observed cell behavior in order to detect and recognize biological or chemical agents affecting the cells. Some possible modelling and classification approaches are presented in this thesis. Advisors/Committee Members: Kolodziej, Wojtek J. (advisor).

Subjects/Keywords: Biosensors

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APA (6^th Edition):

Roussel, N. (2003). Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/30351

Chicago Manual of Style (16^th Edition):

Roussel, Nicolas. “Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents.” 2003. Masters Thesis, Oregon State University. Accessed February 23, 2020. http://hdl.handle.net/1957/30351.

MLA Handbook (7^th Edition):

Roussel, Nicolas. “Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents.” 2003. Web. 23 Feb 2020.

Vancouver:

Roussel N. Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents. [Internet] [Masters thesis]. Oregon State University; 2003. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1957/30351.

Council of Science Editors:

Roussel N. Advanced image segmentation and data clustering concepts applied to digital image sequences featuring the response of biological materials to toxic agents. [Masters Thesis]. Oregon State University; 2003. Available from: http://hdl.handle.net/1957/30351

Oregon State University

4. Hutchison, Janine R. Bacterial pathogens and associated toxins involved in erythrophore cell aggregation.

Degree: PhD, Microbiology, 2009, Oregon State University

URL: http://hdl.handle.net/1957/11976

► Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable,… (more)

▼ Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as a method to detect toxic agents such as chemicals and toxins. The work described here, investigated the potential of erythrophore cells as a detection method for the model food-associated pathogenic bacteria Salmonella enteritidis, Clostridium perfringens, Clostridium botulinum and Bacillus cereus. Erythrophore cell response to each bacterial pathogen was unique and could be distinguished from the bacterial growth medium. These results demonstrate that erythrophore cell response can be used to detect a set of pathogenic bacteria. A second focus of this research effort was to test the hypothesis that erythrophore cells respond to pathogenic bacteria differently then nonpathogenic bacteria. To test this hypothesis, erythrophore cells were exposed to pathogenic and nonpathogenic Bacillus strains. Erythrophore cells failed to respond to Bacillus subtilis and a nonpathogenic Bacillus anthracis vaccine strain. Erythrophore cells responded to members of the B. cereus group including: B. cereus ATCC 10987, B. cereus UW85, B. cereus ATCC 14579, emetic B. cereus NCTC 11143, and Bacillus thuringiensis subsp. kurstaki BGSC 4D1. A third focus of this research effort was to identify the bacterial virulence factor(s) responsible for inducing erythrophore cell response. Random chemical mutagenesis of B. cereus ATCC 49064 resulted in a single amino acid conversion of alanine to threonine at residue 289 of L2 toxin component of HBL. This mutation resulted in a delayed erythrophore cell response which was complemented with the wildtype hbl gene implementing the HBL toxin complex as a factor capable of inducing erythrophore cell response. In conclusion, this study demonstrates that erythrophore cells can be optically monitored to detect several different bacterial pathogens. From this work, the utility of erythrophore cell response can be expanded from function based detection to include studying toxin activity as well as how pathogenic bacteria interact with eukaryotic cells. Advisors/Committee Members: Trempy, Janine E. (advisor), Bermudez, Luiz (committee member).

Subjects/Keywords: Chromatophore; Biosensors

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APA (6^th Edition):

Hutchison, J. R. (2009). Bacterial pathogens and associated toxins involved in erythrophore cell aggregation. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/11976

Chicago Manual of Style (16^th Edition):

Hutchison, Janine R. “Bacterial pathogens and associated toxins involved in erythrophore cell aggregation.” 2009. Doctoral Dissertation, Oregon State University. Accessed February 23, 2020. http://hdl.handle.net/1957/11976.

MLA Handbook (7^th Edition):

Hutchison, Janine R. “Bacterial pathogens and associated toxins involved in erythrophore cell aggregation.” 2009. Web. 23 Feb 2020.

Vancouver:

Hutchison JR. Bacterial pathogens and associated toxins involved in erythrophore cell aggregation. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1957/11976.

Council of Science Editors:

Hutchison JR. Bacterial pathogens and associated toxins involved in erythrophore cell aggregation. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/11976

Oregon State University

5. Dukovcic, Stephanie R. Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances.

Degree: PhD, Microbiology, 2009, Oregon State University

URL: http://hdl.handle.net/1957/12924

► Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application… (more)

▼ Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described as a method to detect toxic agents such as pesticides, chemicals, purified bacterial toxins and food-associated bacteria. The first objective of this study was to examine the B. splendens erythrophore response to Gram-negative food-associated bacteria by investigating the response to Salmonella typhimurium. Erythrophores aggregated in the presence of S. typhimurium in a growth-phase-dependent manner and this response was distinct from the media control. Additionally, it was found that erythrophore aggregation was dependent on the bacterial cell and erythrophores were not responsive to secreted bacterial products found in the culture supernatant. Transposon mutagenesis of S. typhimurium ATCC 700720 resulted in an interruption of the promoter region of operon prgHIJK. This insertion inactivated the expression of the SPI-1 T3SS structural proteins. The mutation resulted in a delayed erythrophore aggregation, implying that the SPI-1 T3SS is an important component contributing to erythrophore aggregation. The second objective of this study was to characterize the Oncorhynchus tshawytscha melanophore in terms of its responsiveness to aggregative and dispersive controls. It was hypothesized that the pigment response was biologically conserved between O. tshawytscha melanophores and B. splendens erythrophores. The data presented support this hypothesis and highlight potential applications. Melanophores aggregated in response to mercuric chloride and sodium arsenite but dispersed in the presence of ammonia. Additionally, melanophores aggregated in response to salmonid bacterial pathogens Aeromonas salmonicida, Yersinia ruckeri and Flavobacterium psychrophilum. Melanophores were capable of differentiating between healthy and diseased fish tissues. These results warrant a continued investigation into the full potential of the O. tshawytscha melanophore system. In conclusion, this study investigated two cell-based biosensor models; B. splendens erythrophores and O. tshawytscha melanophores. The data presented suggests that these models can be applied for the detection of foodborne bacterial pathogens and for the detection of chemical and bacterial contaminants pertinent to the aquaculture industry. Advisors/Committee Members: Trempy, Janine E. (advisor), Lowry, Malcolm B. (committee member).

Subjects/Keywords: Chromatophore; Biosensors

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APA (6^th Edition):

Dukovcic, S. R. (2009). Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/12924

Chicago Manual of Style (16^th Edition):

Dukovcic, Stephanie R. “Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances.” 2009. Doctoral Dissertation, Oregon State University. Accessed February 23, 2020. http://hdl.handle.net/1957/12924.

MLA Handbook (7^th Edition):

Dukovcic, Stephanie R. “Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances.” 2009. Web. 23 Feb 2020.

Vancouver:

Dukovcic SR. Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1957/12924.

Council of Science Editors:

Dukovcic SR. Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/12924

Massey University

6. Goh, Leong Peng. The development of amperometric biosensors for the detection of glucose, lactate and ethanol.

Degree: MS, Biochemistry, 1996, Massey University

URL: http://hdl.handle.net/10179/10544

► Amperometric biosensors, also commonly known as enzyme sensors or enzyme electrodes, are a growing and very progressive area of research. Biosensors are analytical devices that… (more)

▼ Amperometric biosensors, also commonly known as enzyme sensors or enzyme electrodes, are a growing and very progressive area of research. Biosensors are analytical devices that contain a biological sensing element connected to a physical transducing element. The physical transducer "senses" the change in the biological element as it undergoes a chemical reaction. The physical transducer then converts chemical equivalents from the enzyme reaction in a dependent relationship to electrical equivalents that can be measured. Biosensors combine the power of electrochemistry with the specificity of enzymes to produce sensors that are specific to particular enzyme substrates. Some have wide specificities and others are quite narrow. Considering the wide range of enzymes available, the choice depends on the end use of these sensors. The aim of the current study was to design biosensors for the detection of glucose, lactate and ethanol. The method for attaching enzymes to electrodes was based on the carbodiimide method. The carbodiimide method activates haeme which then is able to be covalently attached to enzymes. Enzyme-haeme conjugates were then allowed to absorb onto platinum electrodes by exploiting the knowledge that haeme can bind irreversibly to platinum by sharing pi-electrons with the d-orbitals of platinum. The enzymes involved were glucose oxidase, lactate dehydrogenase and alcohol dehydrogenase. The use of flow injection analysis for evaluating biosensors was desenbed and was found to be a fast, efficient method and the results were highly reproducible. In testing electrodes, the results of the present study showed it was possible to obtain current response that was dependent on the concentration of substrate when these enzyme electrodes were used. A particularly significant result in this study was the achievement of current responses that were dependent on substrate concentration in the absence of NAD+ for lactate and alcohol dehydrogenases using the substrates lactate and ethanol respectively. There is however much work to be done to improve the success rate of making these enzyme electrodes. Several factors were found to cause variable results whilst making and using these enzyme electrodes, such as the absorption of unbound enzyme to the sensing surface of the electrode that may produce significant current response, the formation of aggregated haeme during the enzyme-haeme conjugation process and most importantly, and the ability to make successful enzyme-haeme conjugates to be absorbed onto the sensing surface of the electrodes.

Subjects/Keywords: Biosensors

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APA (6^th Edition):

Goh, L. P. (1996). The development of amperometric biosensors for the detection of glucose, lactate and ethanol. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/10544

Chicago Manual of Style (16^th Edition):

Goh, Leong Peng. “The development of amperometric biosensors for the detection of glucose, lactate and ethanol.” 1996. Masters Thesis, Massey University. Accessed February 23, 2020. http://hdl.handle.net/10179/10544.

MLA Handbook (7^th Edition):

Goh, Leong Peng. “The development of amperometric biosensors for the detection of glucose, lactate and ethanol.” 1996. Web. 23 Feb 2020.

Vancouver:

Goh LP. The development of amperometric biosensors for the detection of glucose, lactate and ethanol. [Internet] [Masters thesis]. Massey University; 1996. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/10179/10544.

Council of Science Editors:

Goh LP. The development of amperometric biosensors for the detection of glucose, lactate and ethanol. [Masters Thesis]. Massey University; 1996. Available from: http://hdl.handle.net/10179/10544

Massey University

7. Large, Ruth. Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University .

Degree: 1993, Massey University

URL: http://hdl.handle.net/10179/14730

► The aim of the following work was to design a biosensor for the detection of ethanol. A biosensor is an analytical device in which a… (more)

Subjects/Keywords: Biosensors

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APA (6^th Edition):

Large, R. (1993). Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University . (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/14730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Large, Ruth. “Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University .” 1993. Thesis, Massey University. Accessed February 23, 2020. http://hdl.handle.net/10179/14730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Large, Ruth. “Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University .” 1993. Web. 23 Feb 2020.

Vancouver:

Large R. Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University . [Internet] [Thesis]. Massey University; 1993. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/10179/14730.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Large R. Development of an amperometric biosensor for the detection of alcohol: a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Biochemistry at Massey University . [Thesis]. Massey University; 1993. Available from: http://hdl.handle.net/10179/14730

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Karapetis, stefanos. Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό.

Degree: 2019, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ)

URL: http://hdl.handle.net/10442/hedi/45413

► The biosensors have important role in science of chemical analysis in the field of electrochemical methods. Their application in rapid detection of toxins in types,… (more)

▼ The biosensors have important role in science of chemical analysis in the field of electrochemical methods. Their application in rapid detection of toxins in types, we consume in our daily lives, is particularly useful for public health. The main characteristics of evaluation of biosensors is the high sensitivity, high selectivity, the quick response and the reusability. Analysis of the real samples need additionally a method of evaluating the rate of recovery that is the fractional difference of signal between real and simulated samples. The advantages of the use of biosensors are part of the easy construction, portability and low cost. The provision of analytical results of biosensors is similar to that of other far more expensive methods of instrumental chemical analysis.In this thesis developed a principle flow injection biosensor (velocity at 2 ml/min) for detection of cholera toxin in aqueous fractions of samples (10 μL or 20 μL).). The biosensor constructed by depositing copper-coated graphene nanoparticles with integrated lipid membranes (BLMs), modified with native GM1 receptors. In the proposed biosensor, regeneration was checked every 6 minutes with 10 successful regenerations retaining its original potency. Control of the biosensor in simulated samples of lake waters was successful and no significant changes were observed under the influence of 7 conventional inhibitors introduced into the samples. The appropriate environment to perform the measurements was set at pH = 7. The biosensor was tested in a dynamic range of the toxin (10nM-10μM) and the detection limit was determined to (LOD = 1nM). This biosensor was studied also in 10 samples of real lake water and the recovery rate was calculated at two concentrations of Toxin (Low 50 nM and High 7 μM). The recovery rates found are acceptable according to the ICH2005 criteria for the suitability of the biosensor for use in repeated water monitoring.Three standard aptasensors were then developed to detect Aflatoxin (AFM1) in milk. The first Label-free aptasensor was made by surface deposition of dendrimer (PAMAM-4G) on a gold electrode where an amino-transformed aptamer -receptor was immobilized. This aptasensor was used as an electrode in the instrumental analysis of the electrochemical impedance spectroscopy (EIS). Control was performed on simulated milk samples at a dynamic range of toxin concentration (15-120 ng/l) and the toxin detection limit was determined at (LOD = 8.47 ng/l). Also, the aptasensor tested in real samples at the same dynamic range with a similar limit of detection and signal recovery greater than 78%.The second aptasensor was made by surface deposition of Neutravidin/Fe-COOH on a gold electrode, where a modified aptamer-receptor with biotin was immobilized. This aptasensor was used as an electrode in the instrumental method of Differential Pulse Voltammetry (DPV). Control was performed on simulated milk samples at a dynamic range of toxin concentration (15-120 ng / l) and the toxin limit of detection was determined at (LOD = 8.52 ng /…

Subjects/Keywords: Βιοαισθητήρες; Biosensors

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APA (6^th Edition):

Karapetis, s. (2019). Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό. (Thesis). National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Retrieved from http://hdl.handle.net/10442/hedi/45413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Karapetis, stefanos. “Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό.” 2019. Thesis, National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Accessed February 23, 2020. http://hdl.handle.net/10442/hedi/45413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Karapetis, stefanos. “Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό.” 2019. Web. 23 Feb 2020.

Vancouver:

Karapetis s. Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό. [Internet] [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2019. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/10442/hedi/45413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Karapetis s. Ανάπτυξη πρότυπων βιοαισθητήτων για τον προσδιορισμό βιοτοξικών ουσιών στο γάλα και στο νερό. [Thesis]. National Technical University of Athens (NTUA); Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ); 2019. Available from: http://hdl.handle.net/10442/hedi/45413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

9. Prakash, Shreya, 1994-. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.

Degree: MS, Electrical and Computer Engineering, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

► Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample… (more)

▼ Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. It provides advantage of shorter processing time, low sample volume and reduced cost per test. Currently, Flow Cytometers are used for blood cells enumeration, however, they are expensive, requires fluorescent tagging of cells and trained technicians to operate the instrument. We propose a non-fluorescent microfluidic architecture with single excitation and detection scheme using the barcoded particles fabricated using Stop Flow Lithography process. The barcoded particles designed with specific number of coding regions can generate numerous distinct patterns of electrical signatures and can be used in biological assays to detect multiple blood cells. A novel design of the asymmetric rectangular barcoded particle was proposed and tested in COMSOL Multiphysics. The barcoded particle with five coding regions generated distinct bi-polar signatures in the microfluidic impedance detection system. Different configurations of the sensing system were proposed to detect the conjugated microspheres (representative of blood cells) effectively based on the site of conjugation. The bottom co-planar electrode and top-bottom electrode configurations were tested for sensitive detection of conjugated microspheres to the barcoded particle. We found that our microfluidic detection system was sensitive enough to detect the presence of a microsphere attached to the barcoded particle. Further, we also investigated the effect of microspheres conjugation orientation to the barcoded particle and their associated electrical signatures. We developed a multi-feature selection algorithm to solve the orientation problem and improved the accuracy of our sensing mechanism. Finally, we fabricated the microfluidic chips using lithography, and co-planar electrodes on glass surfaces using thin film deposition process in the clean room. Our proposed microfluidic system can enumerate multiple blood cells in a single assay using barcoded particles. The concentration of these cells provides useful information about disease onset and progression. Such sensors can be used for diagnostic and management of diseases like Sepsis, Acute Kidney Injury, HIV/ AIDS. Advisors/Committee Members: Hassan, Umer (chair), Gajic, Zoran (internal member), Wu, Chung-Tse Michael (internal member), School of Graduate Studies.

Subjects/Keywords: Microfluidic; Biosensors

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APA (6^th Edition):

Prakash, Shreya, 1. (2019). A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Chicago Manual of Style (16^th Edition):

Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Masters Thesis, Rutgers University. Accessed February 23, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.

MLA Handbook (7^th Edition):

Prakash, Shreya, 1994-. “A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles.” 2019. Web. 23 Feb 2020.

Vancouver:

Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2020 Feb 23]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/.

Council of Science Editors:

Prakash, Shreya 1. A microfluidic biosensing architecture for multiplexed analyte detection using hydrogel barcoded particles. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61911/

Michigan State University

10. Znati, Cynthia Ann. Feasibility study of a biosensor for biological processes.

Degree: MS, Department of Chemical Engineering, 1991, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:22389

Subjects/Keywords: Biosensors

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APA (6^th Edition):

Znati, C. A. (1991). Feasibility study of a biosensor for biological processes. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:22389

Chicago Manual of Style (16^th Edition):

Znati, Cynthia Ann. “Feasibility study of a biosensor for biological processes.” 1991. Masters Thesis, Michigan State University. Accessed February 23, 2020. http://etd.lib.msu.edu/islandora/object/etd:22389.

MLA Handbook (7^th Edition):

Znati, Cynthia Ann. “Feasibility study of a biosensor for biological processes.” 1991. Web. 23 Feb 2020.

Vancouver:

Znati CA. Feasibility study of a biosensor for biological processes. [Internet] [Masters thesis]. Michigan State University; 1991. [cited 2020 Feb 23]. Available from: http://etd.lib.msu.edu/islandora/object/etd:22389.

Council of Science Editors:

Znati CA. Feasibility study of a biosensor for biological processes. [Masters Thesis]. Michigan State University; 1991. Available from: http://etd.lib.msu.edu/islandora/object/etd:22389

11. Barnett, Lauren Marie. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.

Degree: College of Letters & Science, 2017, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/12752

► To see the activity of large, integrated neural circuits functioning in real-time inside of a living brain, neuroscientists will need multiple genetically-encoded fluorescent activity sensors… (more)

Subjects/Keywords: Biosensors.; Brain.; Fluorescence.

11 more images…

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APA (6^th Edition):

Barnett, L. M. (2017). If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/12752

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Barnett, Lauren Marie. “If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.” 2017. Thesis, Montana State University. Accessed February 23, 2020. https://scholarworks.montana.edu/xmlui/handle/1/12752.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Barnett, Lauren Marie. “If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain.” 2017. Web. 23 Feb 2020.

Vancouver:

Barnett LM. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. [Internet] [Thesis]. Montana State University; 2017. [cited 2020 Feb 23]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/12752.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barnett LM. If you build it, they will come: engineering the next generation of optical tools to image neural activity deep within the living brain. [Thesis]. Montana State University; 2017. Available from: https://scholarworks.montana.edu/xmlui/handle/1/12752

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

12. Fogel, Ronen. Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors.

Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2011, Rhodes University

URL: http://hdl.handle.net/10962/d1007166

►

Given their widespread effects and distribution in both natural and industrial environments, the monitoring of phenolic compounds is of considerable analytical interest. Electrochemical biosensor technologies,… (more)

Subjects/Keywords: Laccase; Phenols; Biosensors

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APA (6^th Edition):

Fogel, R. (2011). Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1007166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fogel, Ronen. “Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors.” 2011. Thesis, Rhodes University. Accessed February 23, 2020. http://hdl.handle.net/10962/d1007166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fogel, Ronen. “Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors.” 2011. Web. 23 Feb 2020.

Vancouver:

Fogel R. Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors. [Internet] [Thesis]. Rhodes University; 2011. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/10962/d1007166.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fogel R. Fundamental investigations into the factors affecting the response of laccase-based electrochemical biosensors. [Thesis]. Rhodes University; 2011. Available from: http://hdl.handle.net/10962/d1007166

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

13. Cho, Soohee. Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms .

Degree: 2017, University of Arizona

URL: http://hdl.handle.net/10150/625604

► Diagnostic biosensors are on the rise in the global market due to the increasing prevalence of diseases. Specifically, the point-of-care segment has made great strides… (more)

Subjects/Keywords: Biosensors; Microfluidics; Smartphone

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APA (6^th Edition):

Cho, S. (2017). Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/625604

Chicago Manual of Style (16^th Edition):

Cho, Soohee. “Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms .” 2017. Doctoral Dissertation, University of Arizona. Accessed February 23, 2020. http://hdl.handle.net/10150/625604.

MLA Handbook (7^th Edition):

Cho, Soohee. “Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms .” 2017. Web. 23 Feb 2020.

Vancouver:

Cho S. Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms . [Internet] [Doctoral dissertation]. University of Arizona; 2017. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/10150/625604.

Council of Science Editors:

Cho S. Smartphone-Based Optical Detection of Diagnostic Biosensors on Microfluidic Platforms . [Doctoral Dissertation]. University of Arizona; 2017. Available from: http://hdl.handle.net/10150/625604

Boston University

14. Darlow, Ari. Towards the development of a forensic DNA biosensor.

Degree: MS, Biomedical Forensic Sciences, 2015, Boston University

URL: http://hdl.handle.net/2144/13962

► In the forensic DNA field, quantitative PCR (qPCR) is commonly used to quantify the amount of deoxyribonucleic acid (DNA) in evidentiary samples. Though sensitive, this… (more)

Subjects/Keywords: Chemistry; Biosensors; Forensic

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APA (6^th Edition):

Darlow, A. (2015). Towards the development of a forensic DNA biosensor. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/13962

Chicago Manual of Style (16^th Edition):

Darlow, Ari. “Towards the development of a forensic DNA biosensor.” 2015. Masters Thesis, Boston University. Accessed February 23, 2020. http://hdl.handle.net/2144/13962.

MLA Handbook (7^th Edition):

Darlow, Ari. “Towards the development of a forensic DNA biosensor.” 2015. Web. 23 Feb 2020.

Vancouver:

Darlow A. Towards the development of a forensic DNA biosensor. [Internet] [Masters thesis]. Boston University; 2015. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/2144/13962.

Council of Science Editors:

Darlow A. Towards the development of a forensic DNA biosensor. [Masters Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/13962

Cornell University

15. Matlock-Colangelo, Lauren. Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems .

Degree: 2012, Cornell University

URL: http://hdl.handle.net/1813/29389

► Biosensors detect target analytes through specific binding with biological recognition elements such as nucleic acids, enzymes, and antibodies. Many labs are working to create inexpensive… (more)

▼ Biosensors detect target analytes through specific binding with biological recognition elements such as nucleic acids, enzymes, and antibodies. Many labs are working to create inexpensive and portable miniaturized sensors that allow for rapid sample analysis and low reagent consumption in order to increase biosensor accessibility in rural areas and third world countries. Lab-on-a-chip devices aim to incorporate sample preparation and analyte detection into one device in order to create self-contained sensors that can be used in rural areas and third world countries where laboratory equipment may not be available. Often, these devices incorporate microfluidics in order to shorten reaction times, reduce handling of hazardous samples, and take advantage of laminar flow [1]. However, while several successful lab-on-achip devices have been developed, incorporating sample preparation and analyte detection within one device is still a key challenge in the design of many biosensors. Sample preparation is extremely important for miniaturized sensors, which have a low tolerance for sample impurities and particulates [1]. In addition, significant sample concentration is often required to reduce sample volumes to the nL to mL range used in miniaturized sensors. This research aims to address the need for sample preparation within lab-on-a-chip systems through the use of functionalized electrospun nanofibers within polymer microfluidic devices. Electrospinning is a fiber formation process that uses electrical forces to form fibers with diameters on the order of 100 nm from polymer spinning dopes [2, 3]. The non-woven fiber mats formed during electrospinning have extremely high surface area to volume ratios, and can be used to increase the sensitivity and binding capacity of biosensors without increasing their size. Additionally, the fibers can be functionalized through the incorporation of nano and microscale materials within a polymer spinning dope. In this work, positively and negatively charged v nanofibers were created through the incorporation of hexadimethrine bromide (polybrene) and poly(maleic anhydride) (Poly(MA)) within a poly(vinyl alcohol) spinning dope. Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the successful incorporation of polybrene and poly(MA) into the nanofibers. Gold microelectrodes were patterned on poly(methyl methacrylate) (PMMA) to facilitate the incorporation of nanofibers within microfluidic devices. The gold microelectrodes served as grounded collector plates during electrospinning and produced well-aligned nanofiber mats. Microchannels 1 mm wide and 52 [MICRO SIGN]m deep were imprinted into PMMA through hot embossing with a copper template. PMMA pieces embossed with microchannels were bonded to PMMA pieces with gold microelectrodes and nanofibers using UV-assisted thermal bonding. Positively charged polybrene-modified nanofibers were shown to successfully filter negatively charged fluorescent liposomes out of a HEPES-sucrose-saline buffer, while… Advisors/Committee Members: Kirby, Brian (committeeMember), Frey, Margaret W (committeeMember).

Subjects/Keywords: Biosensors; Nanofibers; Microfluidics

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APA (6^th Edition):

Matlock-Colangelo, L. (2012). Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/29389

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Matlock-Colangelo, Lauren. “Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems .” 2012. Thesis, Cornell University. Accessed February 23, 2020. http://hdl.handle.net/1813/29389.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Matlock-Colangelo, Lauren. “Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems .” 2012. Web. 23 Feb 2020.

Vancouver:

Matlock-Colangelo L. Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems . [Internet] [Thesis]. Cornell University; 2012. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1813/29389.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Matlock-Colangelo L. Functionalized Electrospun Nanofibers In Microfluidic Bioanalytical Systems . [Thesis]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/29389

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oklahoma

16. Godman, Nicholas. Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes.

Degree: PhD, 2015, University of Oklahoma

URL: http://hdl.handle.net/11244/15464

► New methods of constructing enzymatic bioelectrodes based on ferrocene-modified linear poly(ethylenimine) (LPEI) were explored with the intent of lowering device fabrication times. Redox polymers were… (more)

▼ New methods of constructing enzymatic bioelectrodes based on ferrocene-modified linear poly(ethylenimine) (LPEI) were explored with the intent of lowering device fabrication times. Redox polymers were synthesized and characterized for usage as both anode and cathode materials. Photolithography was used to form patterned films based on ferrocenylpropyl-modified linear poly(ethylenimine-co-allylethylenimine) (Fc-C3-LPAEI). Fc-C3-LPAEI (50% allylated, 5% ferrocene) films were crosslinked in the presence of glucose oxidase (GOX) using both photogenerated radicals and nitrenes. Biosensor efficiency was found to be a function of both polymer connectivity and enzyme stability. Fc-C3-LPAEI/GOX bioanodes were capable of generating 44.9 ± 1.3 µAcm-2 after five hours irradiation using a photogenerated dinitrene from 1,2-bis(2-azidoethoxy)ethane. Both electrostatic and covalent layer-by-layer assembly were used for the fabrication of polymer/enzyme composite thin films. Ferrocenylhexyl- and ferrocenylpropyl- modified LPEI (Fc-C6-LPEI, Fc-C3-LPEI. 17-20% ferrocene) were used with periodate modified glucose oxidase (p-GOX) for the construction of enzymatic bioanodes capable of generating up to 381 ± 3 and 1417 ± 63 µAcm-2, respectively. Fc-C3-LPEI/p-GOX biofuel cells generated 86 ± 3 µWcm-2 at pH 7 when poised against an air-breathing Pt cathode. A chloroferrocene-modifed redox polymer (FcCl-C3-LPEI, 17-20% chloroferrocene) was assembled with laccase to construct biocathodes capable of generating up to 5.75 ± 0.14 µAcm-2 on planar gold electrodes and 32.3 ± 3.2 µAcm-2 on nitric acid oxidized carbon paper. Lastly, sulfur and paracyclophane derivatives were copolymerized via inverse vulcanization to afford materials with the possibility of being incorporated in lithium-sulfur batteries. Differential scanning calorimetry was used to monitor the exothermic polymerization between the reactants, and the reaction parameters were optimized by varying the ratios of the starting materials. Advisors/Committee Members: Glatzhofer, Daniel (advisor), Nicholas, Kenneth (committee member), Yip, Wai Tak (committee member), Thomson, Robert (committee member), Crossley, Steven (committee member).

Subjects/Keywords: Biosensors; Electrochemistry; Polymers

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APA (6^th Edition):

Godman, N. (2015). Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/15464

Chicago Manual of Style (16^th Edition):

Godman, Nicholas. “Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes.” 2015. Doctoral Dissertation, University of Oklahoma. Accessed February 23, 2020. http://hdl.handle.net/11244/15464.

MLA Handbook (7^th Edition):

Godman, Nicholas. “Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes.” 2015. Web. 23 Feb 2020.

Vancouver:

Godman N. Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes. [Internet] [Doctoral dissertation]. University of Oklahoma; 2015. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/11244/15464.

Council of Science Editors:

Godman N. Electrochemical Characterization of Photocurable and Layer-By-Layer Assembled Ferrocene-Modified Linear Poly(ethylenimine) Bioelectrodes. [Doctoral Dissertation]. University of Oklahoma; 2015. Available from: http://hdl.handle.net/11244/15464

University of the Western Cape

17. Silwana, Bongiwe. Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor .

Degree: 2012, University of the Western Cape

URL: http://hdl.handle.net/11394/4619

► Environmental pollution is always the hottest topic in public conversation and one of the most concerned aspects of human health. The thin film sputtered microelectrode… (more)

▼ Environmental pollution is always the hottest topic in public conversation and one of the most concerned aspects of human health. The thin film sputtered microelectrode devices have been developed to improve the quality of human health, by offering better monitoring capabilities. This thesis is divided into three parts and the studies were performed on chemical sensor technology currently available and under development using modified methods. In the first part of this thesis: (i) the studies are related to synthesis, characterization and polymerisation of polyaniline (PANI) and polyaniline-co-poly(2,2´-dithiodianline) (PANI-co-PDTDA). Polyaniline (PANI) and the copolymer of aniline with dithiodianiline, an aniline derivative containing S-S-links were of interest in polymer synthesis. Electrochemical synthesis was carried out in 1 M HCl and different concentrations of H2SO4 (1, 2.5, and 5 M) solutions for PANI and PANI-co-PDTDA respectively. The PANI and PANI-co-PDTDA were grown electrochemically on the surface of a glassy carbon electrode (GCE) by repetitive cyclic voltammetric scanning. Cyclic voltammetry (CV) was used to evaluate the differences between the electrochemical characteristics associated with growth of the copolymer and homopolymer, polyaniline (PANI). The surface concentration of PANI was estimated to be 2.64 × 10-1 mol.cm-2 while the film thickness was estimated to be 7.09 × 10-10cm and 1.49 × 10-9cm for scan rate and aquare root scan rate. In contrast, PANI-co-PDTDA concentrations (1, 2, 5 and 5 M H2SO4 solutions) gained a surface concentration (G) falling in the range 6.1 x 10-2 - 7.9 x 102 mol.cm-2 and a film thickness in the range 8.16 x 10 -9- 2.05x10-8cm. The second section of this thesis focused on the development of two sensors, Pt/PANI/HRP and Pt/PANI-co-PDTDA/HRP biosensors. The biosensor described in this chapter focus on the use of horseradish peroxidise (HRP) with hydrogen peroxide as substrate, was constructed with the aim of further investigation of inhibition by heavy metals (Cd2+, Pb2+ and Hg2+). To achieve this, the enzyme HRP as the catalytic bio-element, was immobilised on the surface of a platinum electrode with PANI as a mediator. Immobilisation of HRP in conducting polymer matrices of PANI and PANI-co-PDTDA were achieved by electrochemical polymerisation. The use of amperometric detection allowed for the coupling of the biosensor with a portable potentiostat system (PalmSens). Differential pulse voltammetry (DPV) as technique was used as a detection method for inhibition determination. Selection of suitable pH values for biosensor performance was evaluated and the system showed optimal performance at pH 6.8 and 7.2 for Pt/PANI/HRP and Pt/PANIco- PDTDA/HRP biosensors, respectively. The biosensors developed in this work showed detection limits (LODs) of 0.32 mM and 0.0483 mM for PANI/HRP and PANI-co- PDTDA/HRP, respectively. For the Pt/PANI/HRP biosensor, the apparent Michaelis-Menten constant (Km app) value and maximum current (Imax) were evaluated from Lineweaver-Burk plots at… Advisors/Committee Members: Somerset, V.S (advisor), Iwuoha, Emmanuel (advisor).

Subjects/Keywords: Biosensors; Polyaniline; Nanoparticles

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APA (6^th Edition):

Silwana, B. (2012). Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4619

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silwana, Bongiwe. “Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor .” 2012. Thesis, University of the Western Cape. Accessed February 23, 2020. http://hdl.handle.net/11394/4619.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silwana, Bongiwe. “Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor .” 2012. Web. 23 Feb 2020.

Vancouver:

Silwana B. Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor . [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/11394/4619.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silwana B. Heavy and precious metal toxicity evaluation using a horseradish peroxidase immobilised biosensor . [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/4619

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

18. Zhen, Huajun, 1985-. Investigation of bioaerosol characterization bias due to aerosolization and sampling.

Degree: PhD, Environmental Sciences, 2015, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/47620/

►

Bioaerosols have been investigated for their adverse effects on human health and also their roles in cloud formation, precipitation and atmospheric chemical reactions. In these… (more)

▼

Bioaerosols have been investigated for their adverse effects on human health and also their roles in cloud formation, precipitation and atmospheric chemical reactions. In these studies, it is often necessary to collect biological cells from the airborne state or to disperse the cells into the air using a variety of sampling and aerosolization devices. These devices inevitably exert stress on the cells and result in changes in their biological characteristics, e.g. loss of culturability, impairment of cell membrane, and change in cellular activities. It is highly likely that after experiencing such stress, collected microorganisms are not representative of cells prior to sampling and aerosolization, and thus could introduce biases in their characterization. In this dissertation, the potential bioaerosol characterization biases induced by several commonly used sampling and aerosolization devices were investigated. The stress experienced by E. coli during aerosolization was found to depend on a particular aerosolization device. Particularly, a newly developed pneumatic nebulizer, the Single-Pass Aerosolizer, was shown to better preserve cell culturability and membrane integrity compared to the commonly used Collison nebulizer at similar biological particles output concentrations. Several bioaerosol samplers that employ filtration, impingement, impaction and electrostatic precipitation for sample collection were tested with respect to their effects on the cell membrane integrity and cellular 16S rRNA content of Escherichia coli cells. Sampling stress resulted in severe membrane impairment to E. coli aerosols, leading to the release of genomic DNA as extracellular molecules. Extracellular DNA should be taken into account when analyzing bioaerosol samples to more accurately quantify bacterial presence. Cell membrane damage to bioaerosols depended on which sampler was used and could be reduced by modifying specific operational parameters. E. coli cells exhibited variation in 16S ribosomal RNA (rRNA) level when exposed to long-term air sampling in laboratory experiment, suggesting a change of biological activity in response to sampling stress. The importance of this effect for those taxa in airborne bacterial community from a variety of environments should be examined. The abundance of bacterial 16S rRNA in bioaerosols collected from an outdoor environment was about two orders of magnitude higher than that of 16S rRNA gene. However, the sampler-dependent bias effect on analysis of 16S rRNA sequences for bacterial community composition was minimal in investigated outdoor bioaerosols.

Advisors/Committee Members: Mainelis, Gediminas (chair), Fennell, Donna E (internal member), Reinfelder, John (internal member), SCHWANDER, STEPHAN (outside member).

Subjects/Keywords: Biosensors; Aerosols – Measurement

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APA (6^th Edition):

Zhen, Huajun, 1. (2015). Investigation of bioaerosol characterization bias due to aerosolization and sampling. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/47620/

Chicago Manual of Style (16^th Edition):

Zhen, Huajun, 1985-. “Investigation of bioaerosol characterization bias due to aerosolization and sampling.” 2015. Doctoral Dissertation, Rutgers University. Accessed February 23, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/47620/.

MLA Handbook (7^th Edition):

Zhen, Huajun, 1985-. “Investigation of bioaerosol characterization bias due to aerosolization and sampling.” 2015. Web. 23 Feb 2020.

Vancouver:

Zhen, Huajun 1. Investigation of bioaerosol characterization bias due to aerosolization and sampling. [Internet] [Doctoral dissertation]. Rutgers University; 2015. [cited 2020 Feb 23]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/47620/.

Council of Science Editors:

Zhen, Huajun 1. Investigation of bioaerosol characterization bias due to aerosolization and sampling. [Doctoral Dissertation]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/47620/

Queens University

19. Swyer, Ian. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .

Degree: Chemical Engineering, 2011, Queens University

URL: http://hdl.handle.net/1974/6528

► The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the… (more)

▼ The impedance response of a quadrupolar microelectrode array was studied over a wide frequency range to determine whether particles captured at the center of the array could be detected impedimetrically. The microelectrode array (denoted as DEP chip) uses dielectrophoretic forces to concentrate particles at its center. Initial results showed that there was a large electrode-silicon-electrode (ESE) capacitance which dominated at high frequencies. This capacitance was reduced by decreasing the electrode area and increasing the insulating layer thickness. These measures however proved fruitless as this capacitance was still significantly greater then the dielectric capacitance of the chip. This ESE capacitance can be eliminated through the use of a glass substrate so that the dielectric response of the chip dominates at higher frequencies. Since the ESE capacitance prevented experimental validation of impedance spectroscopy as a signal transduction method, computer simulations were performed. These simulations indicated that capture with the current DEP chips would not have a significant impact on the impedance of the chip. Decreasing the electrode gap distance and reducing the area of the electrodes, which is recommended for future work, can remedy this. As measureable changes in the dielectric capacitance of the chip are not possible, a reaction scheme was developed to translate the capture of viral particles into a change in medium conductivity. An ELISA type system was proposed where the viral particles would be functionalized with urease. This uease would then be used to degrade non-ionic urea into ionic products thereby increasing the medium conductivity. A model was formulated to predict the conductivity increase expected for low concentrations, and validated using higher concentrations of biotinylated-urease. Urease from commercial sources proved not to be a viable option as it does not possess a high enough activity to produce a significant conductivity change given the low concentrations of viral particles expected after collection. Urease with suitable activity is produced by the organism Ureaplasma urealyticum which has an activity of 180 000 µmol urea catalyzed min-1 mg urease-1. It is not recommended that this method be pursued further due to technical challenges that would be encountered.

Subjects/Keywords: Impedance; Biosensors; Microfluidic

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APA (6^th Edition):

Swyer, I. (2011). Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .” 2011. Thesis, Queens University. Accessed February 23, 2020. http://hdl.handle.net/1974/6528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Swyer, Ian. “Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays .” 2011. Web. 23 Feb 2020.

Vancouver:

Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . [Internet] [Thesis]. Queens University; 2011. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1974/6528.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Swyer I. Considerations on the use of Impedance Spectroscopy for the Detection of Virions Trapped in Quadrupolar Microelectrode Arrays . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6528

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

20. Zhu, Hongjie. Intra-body communication channel characterization and modeling.

Degree: 2011, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html

► To fully realize the potentials of the sensors in a personal healthcare system, body area network (BAN) is developed. Existing wireless sensor network technologies are… (more)

Subjects/Keywords: Medical electronics ; Biosensors

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APA (6^th Edition):

Zhu, H. (2011). Intra-body communication channel characterization and modeling. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhu, Hongjie. “Intra-body communication channel characterization and modeling.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed February 23, 2020. http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhu, Hongjie. “Intra-body communication channel characterization and modeling.” 2011. Web. 23 Feb 2020.

Vancouver:

Zhu H. Intra-body communication channel characterization and modeling. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2020 Feb 23]. Available from: http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhu H. Intra-body communication channel characterization and modeling. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7925 ; https://doi.org/10.14711/thesis-b1155530 ; http://repository.ust.hk/ir/bitstream/1783.1-7925/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Bergman, Jenny. Development of Electrochemical Biosensors for Neurochemical Applications.

Degree: 2018, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/54578

► The brain consists of billions of cells, including nerve cells, which have the ability of transforming an incoming electrical signal in to a chemical output… (more)

Subjects/Keywords: biosensors; electrochemistry; neurochemistry

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APA (6^th Edition):

Bergman, J. (2018). Development of Electrochemical Biosensors for Neurochemical Applications. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/54578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bergman, Jenny. “Development of Electrochemical Biosensors for Neurochemical Applications.” 2018. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed February 23, 2020. http://hdl.handle.net/2077/54578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bergman, Jenny. “Development of Electrochemical Biosensors for Neurochemical Applications.” 2018. Web. 23 Feb 2020.

Vancouver:

Bergman J. Development of Electrochemical Biosensors for Neurochemical Applications. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2018. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/2077/54578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bergman J. Development of Electrochemical Biosensors for Neurochemical Applications. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2018. Available from: http://hdl.handle.net/2077/54578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cranfield University

22. Chianella, Iva. Development of affinity sensors for microcystin-LR based on a computationally designed molecularly imprinted polymer.

Degree: PhD, 2003, Cranfield University

URL: http://dspace.lib.cranfield.ac.uk/handle/1826/10744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274017

► In this work the development of affinity sensors for the detection of microcystin-LR based on a computationally designed artificial receptor is presented. Microcystin-LR is a… (more)

▼ In this work the development of affinity sensors for the detection of microcystin-LR based on a computationally designed artificial receptor is presented. Microcystin-LR is a cyclic heptapeptide hepatotoxin produced by Cyanobacteria (aquatic organisms also known as blue-green algae), which during blooms period can release toxins in water. Clinical signs of hepatotoxicosis have been observed in domestic animals and livestock and recently also in humans. At present, analysis of these toxins is achieved largely using conventional, time consuming and expensive techniques such as chromatographic methods (HPLC, TLC) and immunoassay. Therefore, the necessity of an easy and inexpensive method of analysis such a biosensor is becoming urgent. In this work an artificial receptor for microcystin-LR was synthesised using a combined approach of molecular imprinting and computer modelling. A computer-aided rational design was applied to study microcystin-LRlmonomers interactions in order to find an optimal composition for the synthesis of the receptor. The optimised composition, suggested by computer modelling, consisted in 1 mol of2-acrylamido-2-methyl-propanesulfonic acid and 6 mol ofurocanic acid ethyl ester for 1 mol of template. This monomer composition was then used to synthesise a molecularly imprinted polymer (MIP) and an enzyme- linked competitive assay was developed to characterise the computational receptor. In the assay, computational MIP was able both to detect 0.1 ~g rl of microcystin-LR and to distinguish the analyte among analogues such as microcystin-YR, microcystin-RR and nodularin. The computationally designed receptor was then used as a sensing element for the construction of sensor devices. A MIP-based piezoelectric sensor, capable of detecting 35 ~g rl of toxin in water, was developed. In order to improve the system sensitivity, the computational polymer was also used as a material in solid-phase extraction (SPE) for samples pre-concentration. The receptor was able to pre-concentrate up to 1,000 fold tap water samples spiked with only 1 J.1g rl of toxin. By combining MIP-based SPE and piezoelectric sensor an improved system with a minimum detectable concentration of toxin of 0.35 ~g rl was achieved. Encouraging preliminary results were also obtained in developing a MIP-based electrochemical sensor.

Subjects/Keywords: 579; Biosensors

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APA (6^th Edition):

Chianella, I. (2003). Development of affinity sensors for microcystin-LR based on a computationally designed molecularly imprinted polymer. (Doctoral Dissertation). Cranfield University. Retrieved from http://dspace.lib.cranfield.ac.uk/handle/1826/10744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274017

Chicago Manual of Style (16^th Edition):

Chianella, Iva. “Development of affinity sensors for microcystin-LR based on a computationally designed molecularly imprinted polymer.” 2003. Doctoral Dissertation, Cranfield University. Accessed February 23, 2020. http://dspace.lib.cranfield.ac.uk/handle/1826/10744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274017.

MLA Handbook (7^th Edition):

Chianella, Iva. “Development of affinity sensors for microcystin-LR based on a computationally designed molecularly imprinted polymer.” 2003. Web. 23 Feb 2020.

Vancouver:

Chianella I. Development of affinity sensors for microcystin-LR based on a computationally designed molecularly imprinted polymer. [Internet] [Doctoral dissertation]. Cranfield University; 2003. [cited 2020 Feb 23]. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/10744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274017.

Council of Science Editors:

Chianella I. Development of affinity sensors for microcystin-LR based on a computationally designed molecularly imprinted polymer. [Doctoral Dissertation]. Cranfield University; 2003. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/10744 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274017

Cranfield University

23. Subrahmanyam, Sreenath. Design of molecularly imprinted polymers for sensors and solid phase extraction.

Degree: PhD, 2002, Cranfield University

URL: http://dspace.lib.cranfield.ac.uk/handle/1826/7576 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269541

► This thesis presents broadly the applications of molecularly imprinted polymers in sensors and solid phase extraction. Sensors for creatine and creatinine have been reported using… (more)

▼ This thesis presents broadly the applications of molecularly imprinted polymers in sensors and solid phase extraction. Sensors for creatine and creatinine have been reported using a novel method of rational design of molecularly imprinted polymers (MIPs), and solid phase extraction of aflatoxin-B 1 has also been described in the thesis. A method for the selective detection of creataine and creatinine is reported in this thesis, which is based on the reaction between polymerised hemithioacetal, formed by allyl mercaptan, o-phthalic aldehyde, and primary amine leading to the formation of fluorescent isoindole complex. This method was demonstrated for the detection of creatine using creatine-imprinted MIPs. Since MIPs created using traditional methods were unable to differentiate between creatine and creatinine, a new approach to the rational design of a MIP selective for creatinine was developed using computer simulation. A virtual library of functional monomers was assigned and screened against the target molecule, creatinine, using molecular modeling software. The monomers giving the highest binding score were further tested using simulated annealing in order to mimic the complexation of the functional monomers with template in the monomer mixture. The result of this simulation gave an optimised MIP composition. The computationally designed polymer demonstrated superior selectivity in comparison to the polymer prepared using traditional approach, a detection limit of 25 μM and good stability. The 'Bite-and- Switch' approach combined with molecular imprinting can be used for the design of assays and sensors, selective for amino containing substances. MEP for the selective binding properties for aflatoxin-B 1 was prepared using the computational approach. The results obtained demonstrate that the MISPE offers a simple, convenient and a rapid methodology for solid phase extraction of aflatoxin-B 1 even at very low concentrations of 2 ppb. The commercially available C-18 cartridges were able to recover only about 52% of aflatoxin-B 1 at concentrations of 2 ppb when compared with almost complete recovery by the MIP. We have proved here that, MIPs as a solid phase extraction materials offer important and practical advantages with respect to other solid phase extraction methodologies.

Subjects/Keywords: 610; Biosensors

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APA (6^th Edition):

Subrahmanyam, S. (2002). Design of molecularly imprinted polymers for sensors and solid phase extraction. (Doctoral Dissertation). Cranfield University. Retrieved from http://dspace.lib.cranfield.ac.uk/handle/1826/7576 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269541

Chicago Manual of Style (16^th Edition):

Subrahmanyam, Sreenath. “Design of molecularly imprinted polymers for sensors and solid phase extraction.” 2002. Doctoral Dissertation, Cranfield University. Accessed February 23, 2020. http://dspace.lib.cranfield.ac.uk/handle/1826/7576 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269541.

MLA Handbook (7^th Edition):

Subrahmanyam, Sreenath. “Design of molecularly imprinted polymers for sensors and solid phase extraction.” 2002. Web. 23 Feb 2020.

Vancouver:

Subrahmanyam S. Design of molecularly imprinted polymers for sensors and solid phase extraction. [Internet] [Doctoral dissertation]. Cranfield University; 2002. [cited 2020 Feb 23]. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/7576 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269541.

Council of Science Editors:

Subrahmanyam S. Design of molecularly imprinted polymers for sensors and solid phase extraction. [Doctoral Dissertation]. Cranfield University; 2002. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/7576 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269541

University of Alberta

24. Ko, Wooree. Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection.

Degree: MS, Department of Electrical and Computer Engineering, 2009, University of Alberta

URL: https://era.library.ualberta.ca/files/vx021f677

► Nanomechanical resonators offer a pathway towards highly sensitive and label-free detection of biomolecules by transducing the mass of bound analytes into resonant frequency shifts. Zeptogram… (more)

Subjects/Keywords: Nanoelectromechanical systems; Resonators; Biosensors

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APA (6^th Edition):

Ko, W. (2009). Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/vx021f677

Chicago Manual of Style (16^th Edition):

Ko, Wooree. “Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection.” 2009. Masters Thesis, University of Alberta. Accessed February 23, 2020. https://era.library.ualberta.ca/files/vx021f677.

MLA Handbook (7^th Edition):

Ko, Wooree. “Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection.” 2009. Web. 23 Feb 2020.

Vancouver:

Ko W. Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection. [Internet] [Masters thesis]. University of Alberta; 2009. [cited 2020 Feb 23]. Available from: https://era.library.ualberta.ca/files/vx021f677.

Council of Science Editors:

Ko W. Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection. [Masters Thesis]. University of Alberta; 2009. Available from: https://era.library.ualberta.ca/files/vx021f677

University of Edinburgh

25. Ochsenkühn, Michael Andreas. Modern Raman spectroscopy for investigation of host-pathogen interactions.

Degree: PhD, 2010, University of Edinburgh

URL: http://hdl.handle.net/1842/4760

► Biomedical sciences are in need of more versatile and more sensitive approaches for research and also for diagnostic purposes. In particular, intracellular detection and imaging… (more)

▼ Biomedical sciences are in need of more versatile and more sensitive approaches for research and also for diagnostic purposes. In particular, intracellular detection and imaging of disease relevant proteins is a challenge. Although the state of the art method of intracellular imaging is fluorescence, it suffers from several drawbacks. Raman is an alternative imaging modality and this work investigates the use of different Raman techniques for detection and imaging of cellular constituents. In one aspect of the work, surface-enhanced Raman spectroscopy using gold nanoshells excitable at a wavelength of 780 nm was investigated. Initially the investigation of the uptake of the 150 nm diameter nanoparticles showed that NS are taken up voluntarily by a non-standard en- docytosis mechanism into mammalian fibroblast cells. Furthermore it was shown that internalized particles have no detrimental in uence on cell growth or cell viability. That these nanoparticles are non toxic was further confirmed by testing for markers of apoptosis and necrosis. Preliminary surface-enhanced Raman spectroscopy (SERS) studies produced spectra from intracellular compartments with an enhancement factor of 1010. To yield high specificity of the intracellular Raman protein sensor, two different approaches were studied. The first is based on the application of DNA aptamers which form a stacked G-quadruplex on target protein binding. A SERS sensor based on the well characterized Thrombin binding aptamer (TBA) yielded high reproducibility, high target specificity, and a limit of detection down to 0.1 fM. Further studies on a similar stacked G-quadruplex forming aptamer confirmed that observed detection signal is produced by the aptamer assuming its secondary structure but also showed that the stabilization and formation of the G-quadruplex secondary structure is strongly buffer dependent. A second sensing approach was based on a peptide (a3(IV)NC1) influential in Goodpasture's syndrome, an autoimmune disease. With the help of this peptide we found that an intracellular redoxpotential of -200 mV is necessary to make it accessible for the protease Cathepsin D. We found that SERS sensing has the ability to study the binding of Cathepsin D, its activity and with the help of a synthesized amino-acid SERS library the direct detection of the remaining peptide products. Finally this work concludes with imaging the changes of lipid droplet structure and distribution in fibroblast cells during the infection process of the murine cytomegalovirus (MCMV) in fixed and in living cells by coherent anti-Stokes Raman based on a Synchro-lock phase coupled setup. This showed that CARS imaging is able to non-invasively investigate the changes of lipid structures during different stages of the infection process and therefore promises to be a valuable tool in biological research.

Subjects/Keywords: 543.5; biosensors; SERS; CARS; nanoparticles

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APA (6^th Edition):

Ochsenkühn, M. A. (2010). Modern Raman spectroscopy for investigation of host-pathogen interactions. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/4760

Chicago Manual of Style (16^th Edition):

Ochsenkühn, Michael Andreas. “Modern Raman spectroscopy for investigation of host-pathogen interactions.” 2010. Doctoral Dissertation, University of Edinburgh. Accessed February 23, 2020. http://hdl.handle.net/1842/4760.

MLA Handbook (7^th Edition):

Ochsenkühn, Michael Andreas. “Modern Raman spectroscopy for investigation of host-pathogen interactions.” 2010. Web. 23 Feb 2020.

Vancouver:

Ochsenkühn MA. Modern Raman spectroscopy for investigation of host-pathogen interactions. [Internet] [Doctoral dissertation]. University of Edinburgh; 2010. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/1842/4760.

Council of Science Editors:

Ochsenkühn MA. Modern Raman spectroscopy for investigation of host-pathogen interactions. [Doctoral Dissertation]. University of Edinburgh; 2010. Available from: http://hdl.handle.net/1842/4760

26. Ζάβαλη, Μαρία-Δημητρούλα. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.

Degree: 2009, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

►

Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε… (more)

▼

Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε αυτή την αναφορά, παρουσιάζεται μια μεθοδολογία η οποία βασίζεται στη Φασματοσκοπία Ανάκλασης Λευκού Φωτός και ενδείκνυται για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αντιδράσεων που λαμβάνουν χώρα σε στερεά υποστρώματα. Η οπτική διάταξη αποτελείται από μια VIS–NIR οπτική πηγή η οποία επικοινωνεί μέσω οπτικής ίνας με φασματοφωτόμετρο συνδεδεμένο σε Η/Υ. Το εξωτερικό τμήμα της οπτικής ίνας κατευθύνει το φως κάθετα πάνω στην επιφάνεια όπου συμβαίνουν οι αλληλεπιδράσεις μεταξύ των βιιομορίων. Το ανακλώμενο φως συγκεντρώνεται από το εσωτερικό τμήμα της οπτικής ίνας και κατευθύνεται στο φασματοφωτόμετρο. Οι αντιδράσεις λαμβάνουν χώρα σε επιφάνεια διοξειδίου του πυριτίου επικαλυμμένη με πολυμερικό υμένιο. Μια αντλία χρησιμοποιείται για την παροχή των αντιδραστηρίων με ελεγχόμενο ρυθμό σε ένα μικρορρευστομηχανικό κανάλι. Το φάσμα της ανάκλασης καταγράφεται σε πραγματικό χρόνο. Οι αντιδράσεις μεταξύ των πρωτεϊνικών μορίων γίνονται αντιληπτές σαν μετατοπίσεις του μήκους κύματος εκεί που παρατηρείται το φαινόμενο της ενισχυτικής συμβολής. Οι μετατοπίσεις αυτές στο μήκος κύματος συμβαίνουν λόγω του σχηματισμού πρωτεϊνικού στρώματος είτε κατά τη διάρκεια της προσρόφησης των αντισωμάτων στο στερεό υπόστρωμα ή λόγω αλλαγής στο πάχος του πρωτεϊνικού στρώματος όταν τα συμπληρωματικά αντιγόνα δεσμεύονται από τα ήδη ακινητοποιημένα αντισώματα. Η προτεινόμενη μεθοδολογία εφαρμόστηκε για την παρακολούθηση σε πραγματικό χρόνο, χωρίς τη χρήση ιχνηθέτη της αντίδρασης μεταξύ των συμπληρωματικών μορίων βιοτίνης-στρεπταβιδίνης όπως επίσης και για την ανίχνευση της πρόσδεσης των αντιγόνων mouse IgG από ήδη ακινητοποιημένα αντισώματα anti-mouse IgG. Mouse IgG σε συγκεντρώσεις μικρότερες των 150 pM ανιχνεύτηκαν σε πολύ μικρούς χρόνους αντίδρασης (10 min). Ο οπτικός αισθητήρας είναι μια απλή, γρήγορη, χαμηλού κόστους διάταξη για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων που τον καθιστά κατάλληλο για διάφορες αναλυτικές εφαρμογές.

Label-free monitoring of biomolecular reactions in real-time is of great interest since it can provide direct results concerning binding kinetics and equilibrium constants. In this report, a method based on White Light Reflectance Spectroscopy (WLRS) is presented that is capable for real-time monitoring of biomolecular reactions taking place on a solid surface. The optical setup consists of a VIS–NIR light source connected through a bifurcated optical fiber to a PC-driven spectrophotometer. The outer part of the optical fibre guides the light vertically onto the surface where the biomolecular reactions occur. The reflected light is collected from the central part of the optical fibre and is directed to the spectrophotometer. The reactions take place on the top of a polymer covered silicon dioxide surface. A microfluidic module in combination with a micropump is used to supply the…

Advisors/Committee Members: Κουτσούρης, Δημήτριος, Zavali, Maria-Dimitroula, Κουτσούρης, Δημήτριος, Νικήτα, Κωνσταντίνα, Μισιακός, Κωνστατίνος.

Subjects/Keywords: Βιοαισθητήρες; Αντισώματα; 610.28; Biosensors; Antibodies

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APA (6^th Edition):

Ζάβαλη, �. (2009). Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

Chicago Manual of Style (16^th Edition):

Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Masters Thesis, University of Patras. Accessed February 23, 2020. http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.

MLA Handbook (7^th Edition):

Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Web. 23 Feb 2020.

Vancouver:

Ζάβαλη �. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Internet] [Masters thesis]. University of Patras; 2009. [cited 2020 Feb 23]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.

Council of Science Editors:

Ζάβαλη �. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Masters Thesis]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

University of Hong Kong

27. 李丽.; Li, Li, Kirsten. Development of biodetection platform with magnetoresistive sensors andmagnetic nanopartcles.

Degree: PhD, 2013, University of Hong Kong

URL: Li, L. K. [李丽]. (2013). Development of biodetection platform with magnetoresistive sensors and magnetic nanopartcles. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5089979 ; http://dx.doi.org/10.5353/th_b5089979 ; http://hdl.handle.net/10722/192834

► Compared with traditional radioimmunoassay and fluoroimmunoassay for early diseases detection, the magnetic immunoassay utilizing magnetic nanoparticles as bio-labels and magnetic signal sensors as detectors has… (more)

▼ Compared with traditional radioimmunoassay and fluoroimmunoassay for early diseases detection, the magnetic immunoassay utilizing magnetic nanoparticles as bio-labels and magnetic signal sensors as detectors has remarkable advantages because most biological samples exhibit no magnetic background and highly sensitive measurements can be performed. This thesis presents the development of biodetection platform taking advantage of the physical-and chemical-stability, low-toxicity, and environmentally-safety of magnetic iron oxide nanoparticles (IONPs) and the high-sensitivity, low-cost, and portable capabilities of magnetoresistive (MR) sensors. The first part explained why a magnetic biodetection platform is desirable, and what advantages it possesses. Then the magnetism of IONPs utilized in this detection system was introduced, followed by the introduction of main synthesis methods to obtain the desirable IONPs. The working principle of MR sensor was explained, and the recent advances about the biodetection platforms with various magnetoresistive sensors and magnetic IONPs labeling was reviewed. A brief summary of new contributions reported in this thesis was summarized. Then the establishment of home-made measurement setups for the characterization of MR sensor was described. The MR loops of MR sensors can be obtained with the instrument using two-point probe measurement, four-point probe measurement, or Wheatstone bridge measurement. The single MTJ sensor, MTJs array sensor, and the GMR spin valve sensor in Wheatstone bridge were characterized here. The magnetic IONPs were prepared through co-precipitation method and thermal decomposition method, and then surface-functionalized using citric acid and fatty acids to acquire carboxyl groups for the binding ability with biomolecules. The physical and chemical properties, sterilizing-treatment tolerability and biocompatibility of nanoparticles were studied. Furthermore, two new synthesis methods were developed to obtain novel magnetic gold/iron oxide nanocomposites for their potential use as magnetic bio-labels. A magnetic detection platform was built, and the detection of 10-nm superparamagnetic IONPs with MR sensor was first realized here. The output signal of the giant magnetoresistive (GMR) sensor in Wheatstone bridge exhibited log-linear function of the concentration of IONPs, making our sensing system suitable for use when ultra-small bio-labels are needed. The biodetection platform with MR sensor and IONPs was successfully developed and applied for the detection of antigen biomolecules. The feasibility of magnetic biodetection system, based on magnetic tunneling junction (MTJ) sensors and carboxyl-group functionalized IONPs, to detect AFP antigens (liver cancer biomarker) and p24 antigens (HIV biomarker) was demonstrated here for the first time. By taking advantages of its high sensitivity, low power consumption, low cost, and feasibility to be miniaturized, the development of magnetoresistive biodetection platform will bring revolutionary impact on the…

Subjects/Keywords: Magnetoresistance.; Biosensors.; Nanoparticles - Magnetic properties.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

李丽.; Li, Li, K. (2013). Development of biodetection platform with magnetoresistive sensors andmagnetic nanopartcles. (Doctoral Dissertation). University of Hong Kong. Retrieved from Li, L. K. [李丽]. (2013). Development of biodetection platform with magnetoresistive sensors and magnetic nanopartcles. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5089979 ; http://dx.doi.org/10.5353/th_b5089979 ; http://hdl.handle.net/10722/192834

Chicago Manual of Style (16^th Edition):

李丽.; Li, Li, Kirsten. “Development of biodetection platform with magnetoresistive sensors andmagnetic nanopartcles.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed February 23, 2020. Li, L. K. [李丽]. (2013). Development of biodetection platform with magnetoresistive sensors and magnetic nanopartcles. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5089979 ; http://dx.doi.org/10.5353/th_b5089979 ; http://hdl.handle.net/10722/192834.

MLA Handbook (7^th Edition):

李丽.; Li, Li, Kirsten. “Development of biodetection platform with magnetoresistive sensors andmagnetic nanopartcles.” 2013. Web. 23 Feb 2020.

Vancouver:

李丽.; Li, Li K. Development of biodetection platform with magnetoresistive sensors andmagnetic nanopartcles. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2020 Feb 23]. Available from: Li, L. K. [李丽]. (2013). Development of biodetection platform with magnetoresistive sensors and magnetic nanopartcles. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5089979 ; http://dx.doi.org/10.5353/th_b5089979 ; http://hdl.handle.net/10722/192834.

Council of Science Editors:

李丽.; Li, Li K. Development of biodetection platform with magnetoresistive sensors andmagnetic nanopartcles. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Li, L. K. [李丽]. (2013). Development of biodetection platform with magnetoresistive sensors and magnetic nanopartcles. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5089979 ; http://dx.doi.org/10.5353/th_b5089979 ; http://hdl.handle.net/10722/192834

University of Aberdeen

28. Zhang, Bo. Application and evaluation of bacterial biosensors to determine heavy metal bioavailability and assess ecological hazard of soils.

Degree: 2011, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=168276 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553850

► There are significant worries about the impact of heavy metal pollutants in soils during urbanisation and industrialisation in the developing world. Routine chemical analysis of… (more)

▼ There are significant worries about the impact of heavy metal pollutants in soils during urbanisation and industrialisation in the developing world. Routine chemical analysis of soils is used to characterise the concentration of metals translocated by point source and diffuse actions. This fails to put in context the bioavailability or potency of these analytes. Biosensors offer a novel and direct method for hazard assessment of soils impacted with anthropogenic contamination. However, few significant examples of thorough applications of the biosensor in environment have been confirmed. This thesis makes use of comprehensively characterised biosensors for the applications to different environmental matrices. The objective was to apply and evaluate the performance of biosensors in determining the bioavailable fraction of heavy metals in the environment in conjunction with chemical analysis data. Both constitutively marked and metal induced biosensors were optimised and applied, but only the constitutively marked biosensor was responsive to a range of soils and elements of concern. As the matrix that the biosensors were exposed to became increasingly complex, the biosensors' performance became more difficult to interpret. For the metal induced biosensor, the response to standard aqueous samples was predictable as the relationship between chemical analysis data and biosensor predicted data was strong. By contrast, the performance of the metal induced biosensor was poor in soil samples which were historically contaminated (Chapter 9). This could become impacted by the soil characterisation, such as the presence of eo-contaminants, soil associated carbon or variations in soil pH. The constitutively-marked biosensors offered greater predictability of response and this is the reason why these are widely adopted in soil applications. Bacterial biosensors provide a useful tool for assessing the bioavailable fraction of analytes in soils and for complementing chemical analysis. If matrix matched control samples can be collected, then this technology can be applied to a wide range of contrasting soils with a suite of contaminants to aid in the development of generic soil hazard evaluation.

Subjects/Keywords: 628.55; Biosensors : Soils : Soil pollution

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APA (6^th Edition):

Zhang, B. (2011). Application and evaluation of bacterial biosensors to determine heavy metal bioavailability and assess ecological hazard of soils. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=168276 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553850

Chicago Manual of Style (16^th Edition):

Zhang, Bo. “Application and evaluation of bacterial biosensors to determine heavy metal bioavailability and assess ecological hazard of soils.” 2011. Doctoral Dissertation, University of Aberdeen. Accessed February 23, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=168276 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553850.

MLA Handbook (7^th Edition):

Zhang, Bo. “Application and evaluation of bacterial biosensors to determine heavy metal bioavailability and assess ecological hazard of soils.” 2011. Web. 23 Feb 2020.

Vancouver:

Zhang B. Application and evaluation of bacterial biosensors to determine heavy metal bioavailability and assess ecological hazard of soils. [Internet] [Doctoral dissertation]. University of Aberdeen; 2011. [cited 2020 Feb 23]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=168276 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553850.

Council of Science Editors:

Zhang B. Application and evaluation of bacterial biosensors to determine heavy metal bioavailability and assess ecological hazard of soils. [Doctoral Dissertation]. University of Aberdeen; 2011. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=168276 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.553850

University of Minnesota

29. White, Scott. Label-Free, Microfluidic Biosensors with Printed, Floating-Gate Transistors.

Degree: PhD, Chemical Engineering, 2017, University of Minnesota

URL: http://hdl.handle.net/11299/195382

► Printed electronics and microfluidics are two emerging and developing technologies with the common attractive feature of scalability. Advancements in fabrication capabilities have evolved research questions… (more)

▼ Printed electronics and microfluidics are two emerging and developing technologies with the common attractive feature of scalability. Advancements in fabrication capabilities have evolved research questions from, “What can we build?” to, “What should we build?”. This work focuses on the combination of these two technologies and their application to biosensing. The motivating theme is to understand how integrated, functional materials interact, elucidate the underlying molecular phenomena, then utilize the emergent advantages to address the outstanding limitations of conventional biosensing strategies. Printed electronics have recently been applied to biological detection with a variety of techniques1 while microfluidics, since their inception, have been used to handle biological fluids.2 The work presented here outlines a patented sensing strategy based off Floating-Gate Transistors (FGTs). The FGT design physically separates the electronic materials and biological fluids and thus bypasses various compatibility obstacles limiting other next-generation sensor technologies.3 The specific changes in interfacial properties that lead to robust signal transduction are derived empirically.4 This is followed by a mechanistic investigation into the molecular origin of sensor operation when FGTs are used in biomolecular detection. Finally, the versatility and scalability engendered by facile prototyping of FGTs is exemplified by successful iterations to DNA,3 ricin,5 and gluten proteins. The first proof-of-principle experiments incorporated printed electronics with an elementary biological system of DNA oligonucleotides. The results successfully demonstrated the potential of FGTs but failed to solidify their concrete value. Systematic investigation into the complex dynamics at the interface of chemically functionalized electrodes and electrolytes uncovered the most attractive features of the FGT technology. The chemistry was tuned with molecules that range in complexity from simple, short-chain alkyl-thiols to reversible protein-protein interactions. The observed responses with well-controlled systems were generalized to real systems like protein capture in food matrices (e.g. ricin in milk, orange juice). The resulting versatility originated from the label-free, electronic sensing mechanism and opened a range of possibilities for FGTs’ impact. The fundamental insights into interfacial dynamics, device operation, and biomolecular interactions were made possible by the advancements in the materials science and fabrication techniques underlying the presented results. Future avenues of development are hypothesized along with the most promising strategies. The continued elucidation of the physical mechanism and engineering upgrades justify the proposed strategies and inspire the continued effort to fully realize the potential of FGT biosensors.

Subjects/Keywords: Biosensors; Microfluidics; Printed Electronics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

White, S. (2017). Label-Free, Microfluidic Biosensors with Printed, Floating-Gate Transistors. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/195382

Chicago Manual of Style (16^th Edition):

White, Scott. “Label-Free, Microfluidic Biosensors with Printed, Floating-Gate Transistors.” 2017. Doctoral Dissertation, University of Minnesota. Accessed February 23, 2020. http://hdl.handle.net/11299/195382.

MLA Handbook (7^th Edition):

White, Scott. “Label-Free, Microfluidic Biosensors with Printed, Floating-Gate Transistors.” 2017. Web. 23 Feb 2020.

Vancouver:

White S. Label-Free, Microfluidic Biosensors with Printed, Floating-Gate Transistors. [Internet] [Doctoral dissertation]. University of Minnesota; 2017. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/11299/195382.

Council of Science Editors:

White S. Label-Free, Microfluidic Biosensors with Printed, Floating-Gate Transistors. [Doctoral Dissertation]. University of Minnesota; 2017. Available from: http://hdl.handle.net/11299/195382

University of Illinois – Urbana-Champaign

30. Webb, Richard Chad. Flexible, stretchable, biointegrated arrays of electronic thermal sensors and actuators for advancements in clinical medicine.

Degree: PhD, Materials Science & Engr, 2015, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/98322

► Continuous, precision measurements of thermal information related to the human body can provide significant insights into important physiological phenomena, such as blood flow changes, stress,… (more)

▼ Continuous, precision measurements of thermal information related to the human body can provide significant insights into important physiological phenomena, such as blood flow changes, stress, infection and thermoregulation. However, technologies to-date have either been hindered by a high sensitivity to motion artifacts, or have been too bulky and intrusive to be viable for ubiquitous, continuous use outside of a clinic. In addition to mechanical bulk, current skin-mounted technologies for measurements of skin properties do not provide spatial mapping, which is critical to arriving at the most important results. Here we present a class of devices that conform to skin in an intimate, non-intrusive way to provide high precision mapping of temperature and thermal transport signals on skin and other soft tissues. We demonstrate arrays of ultrathin (total thickness <5 µm), flexible, stretchable, skin-conforming devices that map temperatures to a precision (<20 mK) exceeding that of sophisticated infrared cameras for clinical research. We establish the foundational mechanical, electrical and thermal physics and associated design strategies that are necessary for high performance device function. We extend these techniques to the spatial mapping of thermal transport properties on skin, validated in clinical studies at external facilities with comparisons to commercial tools. Additional applications of the physical principles in varied designs enable a new form of minimally invasive continuous blood flow mapping, as well as designs towards the continuous measurement of core body temperature. Specialized mechanical design techniques, which enable reliable transfer printing of devices with arbitrary geometries without sacrificing stretchability, enable additional classes of stretchable electronics with features down to 1.5 µm. Extensions of the design, fabrication and thermal transport principles enable the printing of ultrathin electronic sensor and actuator arrays onto superelastic surgical guidewires down to 350 µm in diameter. Advisors/Committee Members: Rogers, John A. (advisor), Rogers, John A. (Committee Chair), Cahill, David G. (committee member), Cunningham, Brian T. (committee member), Kilian, Kristopher A. (committee member).

Subjects/Keywords: Flexible electronics; Stretchable electronics; Biosensors

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APA (6^th Edition):

Webb, R. C. (2015). Flexible, stretchable, biointegrated arrays of electronic thermal sensors and actuators for advancements in clinical medicine. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/98322

Chicago Manual of Style (16^th Edition):

Webb, Richard Chad. “Flexible, stretchable, biointegrated arrays of electronic thermal sensors and actuators for advancements in clinical medicine.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed February 23, 2020. http://hdl.handle.net/2142/98322.

MLA Handbook (7^th Edition):

Webb, Richard Chad. “Flexible, stretchable, biointegrated arrays of electronic thermal sensors and actuators for advancements in clinical medicine.” 2015. Web. 23 Feb 2020.

Vancouver:

Webb RC. Flexible, stretchable, biointegrated arrays of electronic thermal sensors and actuators for advancements in clinical medicine. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2020 Feb 23]. Available from: http://hdl.handle.net/2142/98322.

Council of Science Editors:

Webb RC. Flexible, stretchable, biointegrated arrays of electronic thermal sensors and actuators for advancements in clinical medicine. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/98322

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Open Access Theses and Dissertations