subject:(Bioremediation)

Clemson University

1. Haluska, Alex. Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride.

Degree: MS, Environmental Engineering and Earth Sciences, 2014, Clemson University

URL: https://tigerprints.clemson.edu/all_theses/3037

► Chlorinated ethenes are known or suspected carcinogens. They are also among the most frequently detected organic groundwater contaminants because of wide use and poor… (more)

▼ Chlorinated ethenes are known or suspected carcinogens. They are also among the most frequently detected organic groundwater contaminants because of wide use and poor disposal practices. Within anaerobic aquifer systems, Fe(III) reduction is a prevalent terminal electron accepting process and has often been reported as a competitive electron acceptor with respect to trichloroethene (TCE), cis-dichloroethene (cis-DCE), and vinyl chloride (VC) reduction. Often, practitioners add excessive amounts of electron donor to compensate for this competition in an effort to avoid incomplete dechlorination or "cis-DCE and VC stall." Contaminated aquifer material incubations were setup to assess dechlorination rates and extent under Fe(III)-reducing conditions, and to evaluate whether excessive electron donor additions increase either the rate or extent of complete dechlorination of TCE or VC using acetate and vegetable oil-based electron donors. Sediment batch experiments were constructed to specifically assess how Fe(III) speciation and the presence of electron shuttles influenced VC dechlorination. Four treatments were investigated: (1) VC +10 mM ferrihydrite; (2) VC + 10 mM Fe(III)-NTA; (3) VC + 10 mM ferrihydrite + 500 µM AQDS; and (4) VC + 5 mM AQDS. Sediment batch experiments were also set up to specifically evaluate how electron donor concentration affected TCE and VC reduction using acetate and vegetable oils (Newman Zone® Standard without sodium lactate, CAP 18 ME, EOS 598B42) as sole electron donors. Three electron donor strategies were investigated for each series. The first donor strategy was operationally defined as "stoichiometric," in which the electron donor concentration was balanced "electron to electron" with either the TCE or VC present and going to ethene. The second and third approaches were an "excessive" electron donor approach in which 5x and 10x the necessary stoichiometric amount of electron donor was added. The results of the Fe(III) speciation and electron shuttle batch study suggest Fe(III) is not inhibitory to complete dechlorination. In all incubations amended with Fe(III), VC was completely reduced to ethene, but the rate of VC reduction was faster in incubations that were not amended with Fe(III), which suggest that the presence of Fe(III) may slow the rate of reductive dechlorination. The results of the high versus low electron donor batch studies demonstrate that TCE and VC were completely degraded when the vegetable oils were amended at stoichiometric and excessive concentrations. Methane accumulations were similar regardless of the treatment applied. TCE and VC reduction were partially degraded in the presence of acetate, regardless of the concentration. Fe(III) reduction did not inhibit ethene production. The data suggest that the addition of substrate may not be necessary if the supply of indigenous electron donor is sufficient and the appropriate microorganisms are present. If electron donor should be added, amendments can start low and be increased. Advisors/Committee Members: Kevin T Finneran, Committee Chair, Cindy Lee, David Freedman.

Subjects/Keywords: Bioremediation

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APA (6^th Edition):

Haluska, A. (2014). Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/3037

Chicago Manual of Style (16^th Edition):

Haluska, Alex. “Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride.” 2014. Masters Thesis, Clemson University. Accessed March 01, 2021. https://tigerprints.clemson.edu/all_theses/3037.

MLA Handbook (7^th Edition):

Haluska, Alex. “Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride.” 2014. Web. 01 Mar 2021.

Vancouver:

Haluska A. Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride. [Internet] [Masters thesis]. Clemson University; 2014. [cited 2021 Mar 01]. Available from: https://tigerprints.clemson.edu/all_theses/3037.

Council of Science Editors:

Haluska A. Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride. [Masters Thesis]. Clemson University; 2014. Available from: https://tigerprints.clemson.edu/all_theses/3037

Oregon State University

2. Li, Jun. Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site.

Degree: MS, Civil Engineering, 2004, Oregon State University

URL: http://hdl.handle.net/1957/29873

Subjects/Keywords: Bioremediation

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APA (6^th Edition):

Li, J. (2004). Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/29873

Chicago Manual of Style (16^th Edition):

Li, Jun. “Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site.” 2004. Masters Thesis, Oregon State University. Accessed March 01, 2021. http://hdl.handle.net/1957/29873.

MLA Handbook (7^th Edition):

Li, Jun. “Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site.” 2004. Web. 01 Mar 2021.

Vancouver:

Li J. Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1957/29873.

Council of Science Editors:

Li J. Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/29873

University of Saskatchewan

3. Rafi, Saif Al. ASSESSMENT OF BIOREMEDIATION FOR TREATMENT OF ARSENIC IN MINE PIT WATER.

Degree: 2020, University of Saskatchewan

URL: http://hdl.handle.net/10388/12880

► Contamination of arsenic (As) in ground water and surface water is a widespread problem throughout the world. Industrial development and increase of anthropogenic activities such… (more)

▼ Contamination of arsenic (As) in ground water and surface water is a widespread problem throughout the world. Industrial development and increase of anthropogenic activities such as mining are an issue of concern due to their pollution of the environment. Because of its toxicity to human and environmental health, remediation of As-contaminated water has become a high priority and a number of As treatment technologies have been developed. Common treatment technologies for As treatment are coagulation, oxidation, filtration processes, electrochemical methods, adsorption, phytoremediation, and bioremediation. Common difficulties with conventional treatment techniques may include the potential production of toxic by-products, limited efficiencies, operational difficulties, and high capital and operation/maintenance costs. Bioremediation may be used to promote the growth of indigenous water and wastewater bacteria, such as sulphate reducing bacteria (SRB), to remove As from these matrices in an effective and environmentally friendly manner. The goal of thesis was to investigate and assess the As bioremediation potential for mine pit water at in situ temperature (8 °C) using molasses as a carbon source. Six sets of experimental batch reactors were prepared including positive controls, negative controls, and molasses amended reactors. A problem with determining As speciation is the need for advanced analytical instruments for the analysis that are not readily available. Thus, suitable sample processing and storage procedures are vital to preserve the species from the time of sampling to analysis. To assess these processing and storage procedures, three methods were used: (1) no acid; (2) ethylene diaminete tetra acetic acid (EDTA); and (3) 2% nitric acid (HNO3). An ion-exchange method was used for the separation of arsenite (As(III)) and arsenate (As(V)) prior to inductively coupled plasma mass spectrometry (ICP-MS), while other metal(loid)s and bacteria were determined using ICP-MS and MiSeq 16S rRNA V4 analyses, respectively. Results showed that iron reducing bacteria increased the As release rate from waste rock over time leading to higher aqueous concentrations in molasses treatment reactors. For the preservation, it was found that filtered samples stored at 4 °C without acidification provided the best results for maintaining appropriate As speciation. Overall, using molasses as a carbon source led to increased As solution concentrations which was not the anticipated outcome. Thus, bioremediation of As in mine pit water needs further investigation and optimization. Advisors/Committee Members: McPhedran, Kerry, Chang, Wonjae, Lindsay, Matt, Abdelrasoul, Amira.

Subjects/Keywords: Arsenic; Bioremediation

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APA (6^th Edition):

Rafi, S. A. (2020). ASSESSMENT OF BIOREMEDIATION FOR TREATMENT OF ARSENIC IN MINE PIT WATER. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12880

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rafi, Saif Al. “ASSESSMENT OF BIOREMEDIATION FOR TREATMENT OF ARSENIC IN MINE PIT WATER.” 2020. Thesis, University of Saskatchewan. Accessed March 01, 2021. http://hdl.handle.net/10388/12880.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rafi, Saif Al. “ASSESSMENT OF BIOREMEDIATION FOR TREATMENT OF ARSENIC IN MINE PIT WATER.” 2020. Web. 01 Mar 2021.

Vancouver:

Rafi SA. ASSESSMENT OF BIOREMEDIATION FOR TREATMENT OF ARSENIC IN MINE PIT WATER. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10388/12880.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rafi SA. ASSESSMENT OF BIOREMEDIATION FOR TREATMENT OF ARSENIC IN MINE PIT WATER. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12880

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

4. Chang, Soon Woong. Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria.

Degree: PhD, Civil Engineering, 1997, Oregon State University

URL: http://hdl.handle.net/1957/33803

► Cometabolic biodegradation processes are potentially useful for the bioremediation of hazardous waste sites. In this study the potential application of phenol-oxidizing and nitrifying bacteria as… (more)

Subjects/Keywords: Bioremediation

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APA (6^th Edition):

Chang, S. W. (1997). Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/33803

Chicago Manual of Style (16^th Edition):

Chang, Soon Woong. “Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria.” 1997. Doctoral Dissertation, Oregon State University. Accessed March 01, 2021. http://hdl.handle.net/1957/33803.

MLA Handbook (7^th Edition):

Chang, Soon Woong. “Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria.” 1997. Web. 01 Mar 2021.

Vancouver:

Chang SW. Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria. [Internet] [Doctoral dissertation]. Oregon State University; 1997. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1957/33803.

Council of Science Editors:

Chang SW. Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria. [Doctoral Dissertation]. Oregon State University; 1997. Available from: http://hdl.handle.net/1957/33803

Rochester Institute of Technology

5. Gallagher, Noreen Anne. Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents.

Degree: MS, Thomas H. Gosnell School of Life Sciences (COS), 2016, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/9296

► Many wastewater treatment plants (WWTPs) are not properly equipped for the removal of various compounds, including non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and hormones. These… (more)

▼ Many wastewater treatment plants (WWTPs) are not properly equipped for the removal of various compounds, including non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and hormones. These compounds are continually discharged into surface waters, which has become an emerging issue for environmental and public health. Microorganisms in the natural environment may play a crucial role in ecosystem self-purification processes such as contaminant degradation. The aim of this research was to determine if there were microorganisms from water and sediment samples located near wastewater effluent outfalls in Central and Western New York capable of degrading ibuprofen, naproxen, acetaminophen, and 17β-estradiol, and if the degradation capability of microorganisms varied by sampling site. An isolation approach was developed using serial enrichment in mineral medium containing each individual pharmaceutical as the sole carbon source available to heterotrophs. After four weeks of enrichment, bacteria were isolated and the growth of each isolate on its selected pharmaceutical source was measured. The biodegradation of pharmaceuticals was then examined with the isolates that showed the most consistent growth. Results from the various enrichment experiments have led to the isolation of several heterotrophic bacteria capable of utilizing the compounds as their sole carbon sources. An isolate cultured from Payne Beach had the ability to remove up to 40.1% ± 3.9% of acetaminophen, 23.2% ± 5.7% of ibuprofen, and 18.6% ± 5.3% of 17β-estradiol and an isolate cultured from Charlotte Beach had the ability to remove up to 23.4% ± 3.5% of ibuprofen, 32.2% ± 2.5% of naproxen, and 29.1% ± 1.9% of 17β-estradiol. The data suggests that there are endogenous heterotrophs located near wastewater outfalls that can degrade various pharmaceuticals, and that the degradation capability of microorganisms on certain compounds may be site specific. Advisors/Committee Members: Jeffrey Lodge.

Subjects/Keywords: Biodegradation; Bioremediation; Pharmaceuticals

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APA (6^th Edition):

Gallagher, N. A. (2016). Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents. (Masters Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/9296

Chicago Manual of Style (16^th Edition):

Gallagher, Noreen Anne. “Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents.” 2016. Masters Thesis, Rochester Institute of Technology. Accessed March 01, 2021. https://scholarworks.rit.edu/theses/9296.

MLA Handbook (7^th Edition):

Gallagher, Noreen Anne. “Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents.” 2016. Web. 01 Mar 2021.

Vancouver:

Gallagher NA. Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents. [Internet] [Masters thesis]. Rochester Institute of Technology; 2016. [cited 2021 Mar 01]. Available from: https://scholarworks.rit.edu/theses/9296.

Council of Science Editors:

Gallagher NA. Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents. [Masters Thesis]. Rochester Institute of Technology; 2016. Available from: https://scholarworks.rit.edu/theses/9296

NSYSU

6. Chang, Chia-Yuan. Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site.

Degree: Master, Environmental Engineering, 2018, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650

► TCE contamination was discovered in 2011 at the research site, and the remediation has been processed since then. After applying KMnO4 for ISCO between 2013… (more)

▼ TCE contamination was discovered in 2011 at the research site, and the remediation has been processed since then. After applying KMnO4 for ISCO between 2013 and 2015, TCE concentration reduced under maximum contaminant level (MCL). However, after stopping injecting substrate, concentration has found bounce back at 4.0 mg/L. To avoid TCE spreading, ground water diffusion control action had been applied between 2009 and 2011 to stopping TCE spreading out of research site. However, the effect to low contamination concentration is limited. Therefore, between 2012 and 2014, two high contamination concentration sites had been selected to apply bioremediation method. Substrate injection can stimulate Dehalococcoides to perform reductive dechlorination of chlorinated ethenes. In that case, concentration will decrease. Objectives of this research included the following (1) Using hydraulic control method to stop contamination from spreading outer research site. (2) Bioremediation: focusing on south of research site at GW1 well to assess long term remediation plan to improve the effective of remediation. (3) Contaminated area monitoring: in conjunction with groundwater contaminated improvement program. Selecting appropriate and existing monitoring wells to exam groundwater quality regularly to develop long term monitoring plan to assess the changes of contamination coverage. The study applied bioremediation method in the entire research site, adopting phased substrate injection with groundwater contamination control. Result on September 2017 showed the TCE contamination concentration were under MCL. GW1 which showed TCE concentration at 0.107 mg/L on February 2016 examine none TCE concentration on September 2017 and last 18 months. Additionally, the changes of contamination coverage had been calculated. Between 2016 and 2017, TCE contamination were lower than 0.05 mg/L. in contamination coverage, TCE contamination volume decreased from 349~702 m3 to 0 m3 indicated the contamination concentration and volume were reduced and under MCL. Consequently, effective of reducing concentration TCE contamination and control contamination coverage were successful in this study. The results indicate that after performing bioremediation process in contaminated source area, the growth of Dehalococcoides can be enhanced, the diffusion of target contaminants in groundwater can be controlled, and the development of bioremediation system to improve the efficiency of TCE reductive dechlorination can be achieved. Advisors/Committee Members: Chih-Ming Kao (committee member), Yao-Ting Tu (chair), Chih-Feng Chen (chair), Chang-Mao Hung (chair).

Subjects/Keywords: Dehalococcoides; bioremediation; TCE

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APA (6^th Edition):

Chang, C. (2018). Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Chia-Yuan. “Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site.” 2018. Thesis, NSYSU. Accessed March 01, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Chia-Yuan. “Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site.” 2018. Web. 01 Mar 2021.

Vancouver:

Chang C. Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site. [Internet] [Thesis]. NSYSU; 2018. [cited 2021 Mar 01]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang C. Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

7. Bowen De Leon, Kara Beth. Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA.

Degree: PhD, College of Letters & Science, 2013, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/3475

► Heavy-metal contamination is a common problem in the industrialized world today. Bioremediation, the use of microorganisms or microbial processes to degrade or reduce contaminants, has… (more)

Subjects/Keywords: Bioremediation.; Chromium.; Nitrates.

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APA (6^th Edition):

Bowen De Leon, K. B. (2013). Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/3475

Chicago Manual of Style (16^th Edition):

Bowen De Leon, Kara Beth. “Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA.” 2013. Doctoral Dissertation, Montana State University. Accessed March 01, 2021. https://scholarworks.montana.edu/xmlui/handle/1/3475.

MLA Handbook (7^th Edition):

Bowen De Leon, Kara Beth. “Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA.” 2013. Web. 01 Mar 2021.

Vancouver:

Bowen De Leon KB. Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA. [Internet] [Doctoral dissertation]. Montana State University; 2013. [cited 2021 Mar 01]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3475.

Council of Science Editors:

Bowen De Leon KB. Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA. [Doctoral Dissertation]. Montana State University; 2013. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3475

Virginia Tech

8. Fahrenfeld, Nicole Leah. Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments.

Degree: PhD, Civil Engineering, 2012, Virginia Tech

URL: http://hdl.handle.net/10919/37811

► The nitroaromatic explosive 2,4,6-trinitrotoluene is a widespread, toxic groundwater contaminant. The objective of this work was to describe TNT fate in contaminated aquifer sediments. A… (more)

▼ The nitroaromatic explosive 2,4,6-trinitrotoluene is a widespread, toxic groundwater contaminant. The objective of this work was to describe TNT fate in contaminated aquifer sediments. A series of bench scale experiments and model simulations were performed to evaluate the fate of TNT in historically contaminated aquifer sediments. A TNT contaminated site on the National Priorities List, Former Nansemond Ordnance Depot (FNOD), Suffolk, VA, served as the model site for this work. To describe desorption rate in contaminated sediments, two approaches for a first order single-site desorption were evaluated. In Model 1, the driving force for desorption is mathematically related to the sorbed phase concentrations, whereas in Model 2 the rate is based on aqueous phase concentrations. Two data sets were used to evaluate the models: (1) batch draw-and-fill experiments using FNOD sediment and (2) results from a previously published report from the Louisiana Army Ammunition Plant. Both models provided adequate fit, but Model 2 was better behaved and first order parameters fell within a smaller confidence interval. Draw-and-fill experiments were observed to yield first-order mass transfer coefficients well aligned with those derived from column experiments. The effect of organic amendments on anaerobic TNT degradation rate and microbial community structure in culture enriched from the FNOD site was studied in batch anaerobic microcosms. TNT readily degraded under all experimental conditions. A reductive pathway of TNT degradation was observed across all conditions, however, denaturing gradient gel electrophoresis (DGGE) analysis revealed distinct bacterial community compositions. In all microcosms, Gram-negative γ- or β-Proteobacteria and Gram-positive Negativicutes or Clostridia were observed. According to non-metric multidimensional scaling analysis of DGGE profiles, the microcosm communities were most similar to field site sediment corresponding to the highest TNT concentration, relative to moderately and uncontaminated sediments, suggesting that TNT contamination itself is a major driver of microbial community structure. Candidate degraders were identified and a Pseudomonas sp. was observed to be stimulated under all conditions, which was confirmed to rapidly degrade TNT in pure culture. Mathematical modeling of the batch microcosm results revealed that TNT degraded 1.7 times faster in lactate amended microcosms than in ethanol amended microcosms, which degraded 3.0 times faster than natural organic matter amended microcosms. Simulation of the TNT degradation pathway included determination of branching coefficients representing whether the first reduction of nitro group occurred in the ortho or para position or whether TNT was removed from the aqueous phase (i.e. bound to dissolved organic matter). Branching coefficients were greater for initial reduction of para (17-27% initial TNT concentration) over ortho (3-9% initial TNT concentration) for all test conditions. However, a greater degradate recovery and a different… Advisors/Committee Members: Schreiber, Madeline E. (committee member), Vikesland, Peter J. (committee member), Widdowson, Mark A. (committeecochair), Pruden, Amy (committeecochair).

Subjects/Keywords: sorption; bioremediation; TNT

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APA (6^th Edition):

Fahrenfeld, N. L. (2012). Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/37811

Chicago Manual of Style (16^th Edition):

Fahrenfeld, Nicole Leah. “Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments.” 2012. Doctoral Dissertation, Virginia Tech. Accessed March 01, 2021. http://hdl.handle.net/10919/37811.

MLA Handbook (7^th Edition):

Fahrenfeld, Nicole Leah. “Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments.” 2012. Web. 01 Mar 2021.

Vancouver:

Fahrenfeld NL. Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments. [Internet] [Doctoral dissertation]. Virginia Tech; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10919/37811.

Council of Science Editors:

Fahrenfeld NL. Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments. [Doctoral Dissertation]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/37811

Rutgers University

9. Heenan, Jeffrey William, 1987-. Geo-electrical monitoring of hydrocarbon degradation.

Degree: PhD, Environmental Science, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61580/

►

The current focus on identifying and remediating contamination calls for further investigation into methods that can be used to efficiently characterize subsurface conditions and monitor… (more)

▼

The current focus on identifying and remediating contamination calls for further investigation into methods that can be used to efficiently characterize subsurface conditions and monitor degradation processes. The primary focus of this research is the latter. Electrical geophysical methods have been well documented as a tool to map aquifers and contaminant plumes, and the same principles that allow for those surveys to be successful allow for these methods to be beneficial for monitoring the progress of remediation. Chemical and physical changes in the subsurface, known to result from biodegradation processes, could be monitored using these methods in situ.This research provides several examples of these methods being effective in monitoring biodegradation. Spectral induced polarization measurements collected over time in a column experiment demonstrate the sensitivity of the method to processes related to microbial induced oil break up, as a result of microbial enhanced oil recovery processes. In another study taking place over the course of approximately 18 months, a progressive decrease in the resistivity of a massive oil spill (Deepwater Horizon) impacted region was observed. Advanced analysis of resistivity variations within the imaged area showed that long-term decreases in resistivity were largely associated with the impacted sediments. Finally, self potential measurements collected at a mature oil spill site, where bioremediation is known to have occurred, showed a measureable, biogeobattery related, response. In all cases, these methods showed the sensitivity of electrical geophysical methods to biodegradation processes. This demonstrates the potential utility of these methods being used an effect monitoring tool to be used in complement with more invasive and expensive chemical/biological testing.

Advisors/Committee Members: Heenan, Jeffrey William, 1987- (author), Ntarlagiannas, Dimitrios (chair), Slater, Lee (internal member), Atekwana, Estella (outside member), Werkema, Dale (outside member).

Subjects/Keywords: Biogeophyiscs; Biodegradation; Bioremediation

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APA (6^th Edition):

Heenan, Jeffrey William, 1. (2017). Geo-electrical monitoring of hydrocarbon degradation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61580/

Chicago Manual of Style (16^th Edition):

Heenan, Jeffrey William, 1987-. “Geo-electrical monitoring of hydrocarbon degradation.” 2017. Doctoral Dissertation, Rutgers University. Accessed March 01, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/61580/.

MLA Handbook (7^th Edition):

Heenan, Jeffrey William, 1987-. “Geo-electrical monitoring of hydrocarbon degradation.” 2017. Web. 01 Mar 2021.

Vancouver:

Heenan, Jeffrey William 1. Geo-electrical monitoring of hydrocarbon degradation. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2021 Mar 01]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61580/.

Council of Science Editors:

Heenan, Jeffrey William 1. Geo-electrical monitoring of hydrocarbon degradation. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61580/

Indian Institute of Science

10. Ebinesar, J S S Allwin. Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications.

Degree: PhD, Faculty of Engineering, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3210

► The rivers, lakes and seas are the major water sources for the animal and plant kingdom in this earth. In recent times, the usage and… (more)

▼ The rivers, lakes and seas are the major water sources for the animal and plant kingdom in this earth. In recent times, the usage and wastage of water have been increasing due to the uncontrolled population growth. In addition to that, the rapid industrialization over the years has led to the gradual depletion of the natural resources like water, soil and air. Some of these industries discharge contaminants like organic products and inorganic (or) toxic heavy metals without treatment into the environment, leading to its degradation. Zinc is the 24th most abundant element present in the earth crust, amounting 75 ppm (0.0075%). The concentration of zinc present in the soil and seawater is about 64 ppm and 30 ppb respectively (Emsley, 2001). Generally, the zinc is found with the base metals such as copper and lead and it has less affinity with oxides and strong affinity with sulphides. Sphalerite, a zinc sulphide ore, is majorly containing 60-62% of zinc. The other sources of zinc from the minerals are smithsonite, hemimorphite, quartzite, and hydro zincate. The major sources of zinc contamination arise from several industrial activities such as mining, coal, waste combustion and steel and iron processing. Drinking water also contains certain amounts of Zn, which may be higher when it is stored in metal tanks. The acute toxicity arises from the ingestion of excessive amounts of zinc salts, either accidentally or as dietary supplement. Vomiting, nausea and stomach cramps usually occur after the consumption of more than 500 mg of zinc sulfate. In addition to that, the higher amounts of zinc affect gastrointestinal tract, liver, bone and prostate glands. Finally, Zn can interrupt the activity in soils, as it negatively influences the activity of microorganisms and earthworms, thus retarding the breakdown of organic matter. To combat this problem, techniques such as chemical precipitation, ion exchange, reverse osmosis, etc. are adopted, but these processes result in a huge amount of secondary sludge formation, inefficient removal of metals and are not cost effective. In recent times, an innovative, eco-friendly, cost-effective method has been introduced to treat the toxic heavy metals namely bioremediation. ―Bioremediation‖ is a process of removal of organic or inorganic contaminants by using bacteria, fungi, algae and its metabolites In this research work, the potential of four bacterial strains of the Pseudomonas sp. such as P.putida, P.alcaligenes, P.aeruginosa and P.fluorescens and the extracellular proteins secreted by these four species for the bio-sorption of zinc has been investigated through batch experiments. The mechanisms of interaction between the zinc ion and the bacterial biomass as well as with the extracellular proteins have been elucidated. Additionally, a carbon paste electrode has been modified by using Pseudomonas sp. and its metabolites to develop biosensors for zinc and the lower limit of detection of zinc in aqueous solution has been determined. The major objectives of this research work are… Advisors/Committee Members: Subramanian, S (advisor).

Subjects/Keywords: Bioremediation; Zinc Bioremediation; Zinc Bioremediation; Toxic Heavy Metals; Bacterial Cell Structure; In-Situ Bioremediation; Ex-Situ Bioremediation; Pseudomonas Species; Materials Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ebinesar, J. S. S. A. (2018). Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3210

Chicago Manual of Style (16^th Edition):

Ebinesar, J S S Allwin. “Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed March 01, 2021. http://etd.iisc.ac.in/handle/2005/3210.

MLA Handbook (7^th Edition):

Ebinesar, J S S Allwin. “Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications.” 2018. Web. 01 Mar 2021.

Vancouver:

Ebinesar JSSA. Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Mar 01]. Available from: http://etd.iisc.ac.in/handle/2005/3210.

Council of Science Editors:

Ebinesar JSSA. Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3210

NSYSU

11. Chiou, Yi-shiou. Development of site investigation and remediation strategies at DNAPLâ Case Study.

Degree: Master, Environmental Engineering, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246

► In this study, the site investigation protocol developed by American Society for Testing and Materials (ASTM) was applied to establish a conceptual model and determine… (more)

Subjects/Keywords: ASTM; tetrachloroethylene; bioremediation; risk assessment

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APA (6^th Edition):

Chiou, Y. (2016). Development of site investigation and remediation strategies at DNAPLâ Case Study. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chiou, Yi-shiou. “Development of site investigation and remediation strategies at DNAPLâ Case Study.” 2016. Thesis, NSYSU. Accessed March 01, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chiou, Yi-shiou. “Development of site investigation and remediation strategies at DNAPLâ Case Study.” 2016. Web. 01 Mar 2021.

Vancouver:

Chiou Y. Development of site investigation and remediation strategies at DNAPLâ Case Study. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Mar 01]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chiou Y. Development of site investigation and remediation strategies at DNAPLâ Case Study. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cape Peninsula University of Technology

12. Santos, Bruno Alexandre Quistorp. Continuous bioremediation of electroplating effluent .

Degree: 2013, Cape Peninsula University of Technology

URL: http://etd.cput.ac.za/handle/20.500.11838/865

► There are significant quantities of free cyanide (F-CN) and heavy metal contaminated effluent being discharged from electroplating operations globally. However, there is an overwhelming tendency… (more)

▼ There are significant quantities of free cyanide (F-CN) and heavy metal contaminated effluent being discharged from electroplating operations globally. However, there is an overwhelming tendency in the industry to use physical and/or chemical treatment methods for cyanides (CNs) and heavy metals in effluent. Although these methods may be effective for certain CNs and heavy metals, they produce toxic by-products and also involve high operational and capital investment costs when compared to bioremediation methods. In this study, the design of a two-stage membrane bioreactor (MBR) system was conceptualised for the bioremediation of CNs and heavy metals in the effluent which was collected from an electroplating facility located in the Western Cape, South Africa. The design included a primary inactive bioremediation stage, to reduce the impact of contaminate concentration fluctuations, and a secondary active bioremediation stage, to remove the residual contaminants, in the effluent under alkaline pH conditions which typify most industrial effluent containing these contaminants. An analysis of the electroplating effluent revealed that the effluent contained an average of 149.11 (± 9.31) mg/L, 5.25 (± 0.64) mg/L, 8.12 (± 4.78) mg/L, 9.05 (± 5.26) mg/L and 45.19 (± 25.89) mg/L of total cyanide (T-CN), F-CN, weak acid dissociable cyanides (WAD-CNs), nickel (Ni), zinc (Zn) and copper (Cu), respectively. An Aspergillus sp., which displayed the characteristic black conidiophores of the Aspergillus section Nigri, was isolated from the electroplating facilities’ effluent discharge using a selective pectin agar (PA) and subcultured on 2% (v/v) antibiotic (10,000 units/L penicillin and 10 mg streptomycin/mL) potato dextrose agar (PDA). The isolate was tolerant to F-CN up to 430 mg F-CN/L on F-CN PDA plates which were incubated at 37 ˚C for 5 days. However, a significant decline in microbial growth was observed after 200 mg F-CN/L, thus indicating that the isolate was suitable for the bioremediation of the electroplating effluent. The identification of the isolate as Aspergillus awamori (A. awamori) was definitively determined using a multi-gene phylogenetic analysis, utilising ITS (internal transcribed spacer), -tubulin and calmodulin gene regions. Although an anomaly in the morphology of the conidia of the isolate was observed during the morphological analysis, indicating a possible morphological mutation in the isolate. A comparative study between “sweet orange” (Citrus sinensis (C. sinensis)) pomace, “apple” (Malus domestica (M. domestica)) pomace, “sweetcorn” (Zea mays (Z. mays)) cob and “potato” (Solanum tuberosum (S. tuberosum)) peel, i.e. waste materials considered to be agricultural residues, was conducted in order to assess their potential and as a sole carbon source supplement for A. awamori biomass development for the bioremediation of CNs and heavy metals. The suitability of these agricultural residues for these activities were as follows: C. sinensis pomace ˃ M. domestica pomace ˃ Z. mays cob ˃ S. tuberosum peel. For…

Subjects/Keywords: Bioremediation; Electroplating; Dissertations, Academic; MTech

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APA (6^th Edition):

Santos, B. A. Q. (2013). Continuous bioremediation of electroplating effluent . (Thesis). Cape Peninsula University of Technology. Retrieved from http://etd.cput.ac.za/handle/20.500.11838/865

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santos, Bruno Alexandre Quistorp. “Continuous bioremediation of electroplating effluent .” 2013. Thesis, Cape Peninsula University of Technology. Accessed March 01, 2021. http://etd.cput.ac.za/handle/20.500.11838/865.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santos, Bruno Alexandre Quistorp. “Continuous bioremediation of electroplating effluent .” 2013. Web. 01 Mar 2021.

Vancouver:

Santos BAQ. Continuous bioremediation of electroplating effluent . [Internet] [Thesis]. Cape Peninsula University of Technology; 2013. [cited 2021 Mar 01]. Available from: http://etd.cput.ac.za/handle/20.500.11838/865.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santos BAQ. Continuous bioremediation of electroplating effluent . [Thesis]. Cape Peninsula University of Technology; 2013. Available from: http://etd.cput.ac.za/handle/20.500.11838/865

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rochester Institute of Technology

13. Delp, Danielle. Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds.

Degree: MS, Thomas H. Gosnell School of Life Sciences (COS), 2015, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/8894

► Anthropogenic nutrient pollution is one of the most serious threats facing aquatic systems in modern times. Pollution with compounds containing nitrogen and phosphorous encourages… (more)

Subjects/Keywords: Algae; Biofilm; Bioremediation; Chlorella; Eutrophication

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APA (6^th Edition):

Delp, D. (2015). Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds. (Masters Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/8894

Chicago Manual of Style (16^th Edition):

Delp, Danielle. “Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds.” 2015. Masters Thesis, Rochester Institute of Technology. Accessed March 01, 2021. https://scholarworks.rit.edu/theses/8894.

MLA Handbook (7^th Edition):

Delp, Danielle. “Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds.” 2015. Web. 01 Mar 2021.

Vancouver:

Delp D. Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds. [Internet] [Masters thesis]. Rochester Institute of Technology; 2015. [cited 2021 Mar 01]. Available from: https://scholarworks.rit.edu/theses/8894.

Council of Science Editors:

Delp D. Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds. [Masters Thesis]. Rochester Institute of Technology; 2015. Available from: https://scholarworks.rit.edu/theses/8894

Cornell University

14. Posman, Kevin. Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3.

Degree: M.S., Microbiology, Microbiology, 2013, Cornell University

URL: http://hdl.handle.net/1813/34294

▼ © 2013 Kevin M. Posman ! ABSTRACT Microbial processes shape ecosystem functions and the fate of environmental Contaminants. Despite their importance little is known about the active players carrying out specific metabolic processes in the environment. Investigations of environmental microbial communities are crucial for understanding the diversity of microorganisms capable of degrading these compounds and will provide insight into bioremediation strategies. The aim of this study was to use cultivation-dependent and cultivation-independent techniques to examine benzenedegrading microorganisms in hydrocarbon-contaminated groundwater. We investigated groundwater samples from a coal-tar contaminated aquifer in South Glens Falls, NY undergoing monitored natural attenuation (MNA). Previous stable isotope probing (SIP) experiments using 13 C-labelled benzene in groundwater microcosms from the site revealed a high abundance of !- and "-proteobacteria were active in the biodegradation of benzene. To further explore the microbiology of this system, we isolated benzene-degrading microorganisms from the SIP microcosm experiments. Nine organisms were isolated and identified using 16S rRNA gene sequences and several shared >97% nucleotide identity to 16S rRNA gene sequences retrieved in the SIP experiments. Isolate Variovorax MAK3 was chosen for further characterization. We designed primers to investigate a putative ring-hydroxylating dioxygenase (RHD) hypothesized to be involved in its ability to degrade benzene. We demonstrated that Variovorax MAK3 accelerated benzene degradation microcosms prepared from site waters and quantified a corresponding increase in Variovorax RHD gene expression over the same time period. Finally, we show that when the native community was exposed to benzene the ratio of RDH dioxygenase to 16S rRNA gene transcripts of native Variovorax populations increased over 6 fold during the benzene biodegradation. These data demonstrate how the convergence of cultivation-dependent ! and cultivation-independent techniques can lead to a precise understanding of active populations and their biodegradation genes in complex microbial communities. ! Advisors/Committee Members: Madsen, Eugene Lewis (chair), Hewson, Ian (committee member).

Subjects/Keywords: microbiology; qPCR; benzene; bioremediation

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APA (6^th Edition):

Posman, K. (2013). Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3. (Masters Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/34294

Chicago Manual of Style (16^th Edition):

Posman, Kevin. “Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3.” 2013. Masters Thesis, Cornell University. Accessed March 01, 2021. http://hdl.handle.net/1813/34294.

MLA Handbook (7^th Edition):

Posman, Kevin. “Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3.” 2013. Web. 01 Mar 2021.

Vancouver:

Posman K. Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3. [Internet] [Masters thesis]. Cornell University; 2013. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1813/34294.

Council of Science Editors:

Posman K. Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3. [Masters Thesis]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34294

Queens University

15. Grell, Stephanie Louise. Heated Biofilm Growth in a Planar Fracture for Reduction of Hydraulic Aperture .

Degree: Civil Engineering, 2011, Queens University

URL: http://hdl.handle.net/1974/6624

► The objective of this study was to examine the effect of heat on the growth of a biofilm in a parallel glass plate fracture table.… (more)

▼ The objective of this study was to examine the effect of heat on the growth of a biofilm in a parallel glass plate fracture table. Groundwater was collected from a limestone aquifer and amended with a nutrient mixture to stimulate the indigenous microbial population. The amended water was heated to approximately 30 °C in an upgradient reservoir attached to the fracture table and recirculated through the 2-m long, 0.6-m wide, parallel glass plate fracture having an approximate fracture aperture of 2000 μm. The fracture was maintained at approximately 10 °C to simulate natural in situ groundwater temperature and the upgradient reservoir maintained at 30 °C. Geochemical parameters and bacterial counts were measured regularly throughout the biostimulation to monitor biofilm growth in the fracture. Hydraulic tests and tracer experiments completed before and after the biostimulation were used as the primary indicators of the successful bioclogging of the fracture. Geochemical parameters measured throughout the trial revealed an increasingly reducing environment capable of supporting the development of a diverse biofilm. Direct and indirect bacterial counts revealed the dominant bacteria within the system included common groundwater bacteria pseudonomads, enteric, and slime-forming bacteria. Heterotrophic bacteria were also present in significant concentrations. Visible clusters of biofilm were observed on Day 2 of the trial with a fully-connected biofilm observed by Day 7. The biofilm impacted the groundwater flow through the fracture resulting in an approximately 2.75-hour delay in the tracer’s breakthrough during the tracer experiment completed on Day 13 of the trial compared to an experiment conducted during the initial stages of biofilm development. Based on the results of the tracer experiment, the biofilm growth reduced the velocity of the groundwater by 9.8%, the fracture aperture by 37.8%, and increased the bulk dispersivity to 50mm. Recommendations for future work include the application of heated biostimulation at the field scale in a well-characterized, isolated fracture.

Subjects/Keywords: Hydrogeology ; Bioremediation ; Fractured Bedrock

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APA (6^th Edition):

Grell, S. L. (2011). Heated Biofilm Growth in a Planar Fracture for Reduction of Hydraulic Aperture . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Grell, Stephanie Louise. “Heated Biofilm Growth in a Planar Fracture for Reduction of Hydraulic Aperture .” 2011. Thesis, Queens University. Accessed March 01, 2021. http://hdl.handle.net/1974/6624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Grell, Stephanie Louise. “Heated Biofilm Growth in a Planar Fracture for Reduction of Hydraulic Aperture .” 2011. Web. 01 Mar 2021.

Vancouver:

Grell SL. Heated Biofilm Growth in a Planar Fracture for Reduction of Hydraulic Aperture . [Internet] [Thesis]. Queens University; 2011. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1974/6624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Grell SL. Heated Biofilm Growth in a Planar Fracture for Reduction of Hydraulic Aperture . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

16. Huang, Xiaoyan. Characterization and genome-scale metabolic modeling of catechol-degrading Pseudomonas fluorescens isolated from a petroleum hydrocarbon-impacted site.

Degree: 2020, University of Saskatchewan

URL: http://hdl.handle.net/10388/12813

► Pseudomonas fluorescens is a candidate for efficient petroleum hydrocarbons (PHC) biodegradation. In this work, a P. fluorescens strain was isolated from a local PHC-impacted site.… (more)

▼ Pseudomonas fluorescens is a candidate for efficient petroleum hydrocarbons (PHC) biodegradation. In this work, a P. fluorescens strain was isolated from a local PHC-impacted site. To investigate its PHC biodegradation performance, catechol, an important metabolic intermediate during monoaromatic hydrocarbon biodegradation, was chosen as the sole carbon source. A set of experiments based on a 23 factorial design was undertaken to investigate how nitrate, sulfate, and phosphate ions affect catechol biodegradation by the isolated P. fluorescens strain. The experimental results were subjected to ANOVA. Maximum specific catechol degradation rates (the response) were estimated by a three-parameter logistic model to evaluate bioremediation performance. ANOVA results suggest introducing nitrate ions alone may lead to poorer bioremediation performance, introducing sulfate ions alone does not affect bioremediation performance, but supplementing with nitrate and sulfate ions together can enhance bioremediation performance. P. fluorescens was also shown to survive under sulfur-limited conditions. Injecting phosphate ions also led to better bioremediation performance. To gain extensive and systematic knowledge of P. fluorescens, the first genome-scale metabolic model (GSMM) for P. fluorescens was reconstructed, termed lCW1057. The model was validated by in vitro growth data. The periplasmic compartment was constructed to better represent the proton gradient profile. The reconstructed proton transport chain has a P/O ratio of 11/8. Flux balance analysis (FBA) was performed to simulate the whole-cell metabolic flow. The simulation results suggested the β-ketoadipate pathway is involved in catechol metabolism by P. fluorescens while the uptake of oxygen is mandatory for cleavage of catechol’s aromatic ring. The Entner-Doudoroff (ED) pathway was involved in glycolysis for P. fluorescens. Moreover, nitrates can be used as the terminal electron acceptor to support P. fluorescens growth under anaerobic condition. Advisors/Committee Members: Lin, Yen-Han, Wang, Hui, Xiong, WenHui, Chang, WonJae.

Subjects/Keywords: Bioremediation; Catechol; Pseudomonas fluorescens

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APA (6^th Edition):

Huang, X. (2020). Characterization and genome-scale metabolic modeling of catechol-degrading Pseudomonas fluorescens isolated from a petroleum hydrocarbon-impacted site. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12813

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Xiaoyan. “Characterization and genome-scale metabolic modeling of catechol-degrading Pseudomonas fluorescens isolated from a petroleum hydrocarbon-impacted site.” 2020. Thesis, University of Saskatchewan. Accessed March 01, 2021. http://hdl.handle.net/10388/12813.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Xiaoyan. “Characterization and genome-scale metabolic modeling of catechol-degrading Pseudomonas fluorescens isolated from a petroleum hydrocarbon-impacted site.” 2020. Web. 01 Mar 2021.

Vancouver:

Huang X. Characterization and genome-scale metabolic modeling of catechol-degrading Pseudomonas fluorescens isolated from a petroleum hydrocarbon-impacted site. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10388/12813.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang X. Characterization and genome-scale metabolic modeling of catechol-degrading Pseudomonas fluorescens isolated from a petroleum hydrocarbon-impacted site. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12813

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

17. Ilić, Dora, 1974- 23405671. Hrom-redukujući mikroorganizmi u bioremedijaciji zemljišta zagađenog teškim metalima.

Degree: Poljoprivredni fakultet, 2020, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:20761/bdef:Content/get

►

Mikrobiologija - Ekološka mikrobiologija / Microbiology - Microbial ecology

Mnogobrojne industrijske aktivnosti, osim koristi za ljudsku populaciju, imale su za posledicu povećanje sadržaja teških metala… (more)

Subjects/Keywords: microorganisms; bioremediation; heavy metals; chromium

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APA (6^th Edition):

Ilić, Dora, 1. 2. (2020). Hrom-redukujući mikroorganizmi u bioremedijaciji zemljišta zagađenog teškim metalima. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:20761/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ilić, Dora, 1974- 23405671. “Hrom-redukujući mikroorganizmi u bioremedijaciji zemljišta zagađenog teškim metalima.” 2020. Thesis, Univerzitet u Beogradu. Accessed March 01, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:20761/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ilić, Dora, 1974- 23405671. “Hrom-redukujući mikroorganizmi u bioremedijaciji zemljišta zagađenog teškim metalima.” 2020. Web. 01 Mar 2021.

Vancouver:

Ilić, Dora 12. Hrom-redukujući mikroorganizmi u bioremedijaciji zemljišta zagađenog teškim metalima. [Internet] [Thesis]. Univerzitet u Beogradu; 2020. [cited 2021 Mar 01]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:20761/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ilić, Dora 12. Hrom-redukujući mikroorganizmi u bioremedijaciji zemljišta zagađenog teškim metalima. [Thesis]. Univerzitet u Beogradu; 2020. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:20761/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

18. Ennis, Elliot G. Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater.

Degree: MS, Chemistry, 2005, Oregon State University

URL: http://hdl.handle.net/1957/22656

► Chlorinated aliphatic hydrocarbons (CAHs) such as vinyl chloride (VC) and 1,-2 dichioroethane (DCA), are wide-spread groundwater pollutants found at many contaminated field sites around the… (more)

Subjects/Keywords: Bioremediation – Evaluation

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APA (6^th Edition):

Ennis, E. G. (2005). Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/22656

Chicago Manual of Style (16^th Edition):

Ennis, Elliot G. “Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater.” 2005. Masters Thesis, Oregon State University. Accessed March 01, 2021. http://hdl.handle.net/1957/22656.

MLA Handbook (7^th Edition):

Ennis, Elliot G. “Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater.” 2005. Web. 01 Mar 2021.

Vancouver:

Ennis EG. Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater. [Internet] [Masters thesis]. Oregon State University; 2005. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1957/22656.

Council of Science Editors:

Ennis EG. Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater. [Masters Thesis]. Oregon State University; 2005. Available from: http://hdl.handle.net/1957/22656

University of Connecticut

19. Zhang, Xiaolong. Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil.

Degree: MS, Environmental Engineering, 2011, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/40

► Hexavalent chromium (Cr(VI)) has been identified as a high priority pollutant by the U.S. Environmental Protection Agency because of its high toxicity and carcinogenicity.… (more)

▼ Hexavalent chromium (Cr(VI)) has been identified as a high priority pollutant by the U.S. Environmental Protection Agency because of its high toxicity and carcinogenicity. Cr(VI) is commonly used in electroplating, pigment, leather processing and mining resulting in widespread Cr(VI) pollution, and a need for the development of cost-effective remediation technologies. Microbial reduction of Cr(VI) to non-toxic trivalent chromium (Cr(III)) is a promising technique. In this study, five bacteria were isolated from the soil of Cr(VI) contaminated plating facility in Putnam, CT. Then were identified tentatively as Leucobacter sp., Serratia sp., Achromobacter sp., Delftia sp. and Pseudomonas fluorescens using the 16S rRNA technique. The biological reduction of aqueous Cr(VI) was initially evaluated in mixed culture of the five bacteria. pH, temperature and initial Cr(VI) concentration had a significant effect on Cr(VI) reduction. The highest Cr(VI) reduction rate was obtained at pH=8, 25-30°C and an initial Cr(VI) concentration less than 50mg/L. Approximately 98% of 40 mg/L Cr(VI) was reduced within 48 hours culture at these conditions. Cr(VI) reduction was shown to correlate with bacterial growth. Bio-adsorption experiment confirmed that the decrease in Cr(VI) not due to bio-adsorption, and bio-reduction was proved by further chromium analysis. Scanning Electron Microscopy was also employed to observe the morphology of the bacteria in cultures with and without Cr(VI). Finally, Pseudomonas fluorescens was used to evaluate Cr(VI) reduction in sterilized and live soil samples. When added at a dosage of 30 mg Cr(VI) per kg dry soil, all Cr(VI) was reduced within one day of curing without the addition of an external carbon source. At an addition of 500 mg/kg Cr(VI), 60% of Cr(VI) was reduced in the sterilized soil within 5 days with 10% (w/w) peptone added, while around 22% Cr(VI) reduction was achieved in the sterilized soil without peptone added. Compared to the sterilized soil, the Cr(VI) reduction rate was slightly increased in the live soil with peptone added. Advisors/Committee Members: Baikun Li;Jose Amador, Maria Chrysochoou.

Subjects/Keywords: hexavalent chromium; aerobic bacteria; bioremediation

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APA (6^th Edition):

Zhang, X. (2011). Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/40

Chicago Manual of Style (16^th Edition):

Zhang, Xiaolong. “Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil.” 2011. Masters Thesis, University of Connecticut. Accessed March 01, 2021. https://opencommons.uconn.edu/gs_theses/40.

MLA Handbook (7^th Edition):

Zhang, Xiaolong. “Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil.” 2011. Web. 01 Mar 2021.

Vancouver:

Zhang X. Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil. [Internet] [Masters thesis]. University of Connecticut; 2011. [cited 2021 Mar 01]. Available from: https://opencommons.uconn.edu/gs_theses/40.

Council of Science Editors:

Zhang X. Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil. [Masters Thesis]. University of Connecticut; 2011. Available from: https://opencommons.uconn.edu/gs_theses/40

Colorado State University

20. Zeman, Natalie Rae. Thermally enhanced bioremediation of LNAPL.

Degree: MS(M.S.), Civil and Environmental Engineering, 2013, Colorado State University

URL: http://hdl.handle.net/10217/79208

► Inadvertent releases of petroleum liquids into the environment have led to widespread soil and groundwater contamination. Petroleum liquids, referred to as Light Non-Aqueous Phase Liquid… (more)

▼ Inadvertent releases of petroleum liquids into the environment have led to widespread soil and groundwater contamination. Petroleum liquids, referred to as Light Non-Aqueous Phase Liquid (LNAPL), pose a threat to the environment and human health. The purpose of the research described herein was to evaluate thermally enhanced bioremediation as a sustainable remediation technology for rapid cleanup of LNAPL zones. Thermally enhanced LNAPL attenuation was investigated via a thermal microcosm study that considered six different temperatures: 4¡ÆC, 9¡ÆC, 22¡ÆC, 30¡ÆC, 35¡ÆC, and 40¡ÆC. Microcosms were run for a period of 188 days using soil, water and LNAPL from a decommissioned refinery in Evansville, WY. The soil microcosms simulated anaerobic subsurface conditions where sulfate reduction and methanogenesis were the pathways for biodegradation. To determine the optimal temperature range for thermal stimulation and provide guidance for design of field-scale application, both contaminant degradation and soil microbiology were monitored. CH4 and CO2 generation occurred in microcosms at 22¡ÆC, 30¡ÆC, 35¡ÆC and 40¡ÆC but was not observed at 4¡ÆC and 9¡ÆC. The total volume of biogas generated after 188 days of incubation was 19 times higher in microcosms at 22¡ÆC and 30¡ÆC compared to the microcosms at 35¡ÆC. When compared to microcosms at 40¡ÆC, the total biogas generated was 3 times higher at both 22¡ÆC and 30¡ÆC. The onset of CH4 and CO2 production occurred first within the microcosms held at 30¡ÆC beginning after 28 days of incubation, and second within microcosms at 22¡ÆC beginning after 58 days of incubation. A delay in CH4 and CO2 production was observed within microcosms held at 35¡ÆC and 40¡ÆC (beginning after 173 and 138 days after incubation) contributing to lower cumulative biogas generation at these temperatures relative to 22¨¬C and 30¨¬C. Microcosms incubated at 4¨¬C, 22¡ÆC, 30¨¬C, 35 ¨¬C and 40 ¨¬C showed statistically significant biological removal of gasoline range organics (GRO) at the ¥á=0.05 level over the course of the 188-day incubation period. Biotic removal of GRO was significantly higher at 22¨¬C and 30¨¬C when compared to 4¨¬C and 9¨¬C. The observed removals at 22 ¨¬C, 30 ¨¬C, 35 ¨¬C and 40¨¬C were not statistically different from each other. The biological removal of DRO compounds was found to be statistically significant at 22¨¬C, 30 ¨¬C, 35 ¨¬C and 40 ¨¬C and was significantly higher at 22¨¬C when compared to 4¨¬C and 9¨¬C. The percent biological removal of DRO compounds at these temperatures was not statistically different from each other and ranged from 18-22%. Statistically significant biological degradation of all the BTEX compounds only occurred in microcosms at 22¡ÆC and 30¡ÆC. Benzene, toluene, and xylene biodegradation was observed to be statistically significant in microcosms at 35¡ÆC. Phylogenetic analysis of the microbial communities present at 22¡ÆC via a 16S rRNA gene archaeal clone library and pyrosequencing revealed the presence of both hydrogenotrophic and… Advisors/Committee Members: De Long, Susan K. (advisor), Sale, Thomas (advisor), Stromberger, Mary (committee member).

Subjects/Keywords: thermal; bioremediation; anaerobic; LNAPL

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APA (6^th Edition):

Zeman, N. R. (2013). Thermally enhanced bioremediation of LNAPL. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/79208

Chicago Manual of Style (16^th Edition):

Zeman, Natalie Rae. “Thermally enhanced bioremediation of LNAPL.” 2013. Masters Thesis, Colorado State University. Accessed March 01, 2021. http://hdl.handle.net/10217/79208.

MLA Handbook (7^th Edition):

Zeman, Natalie Rae. “Thermally enhanced bioremediation of LNAPL.” 2013. Web. 01 Mar 2021.

Vancouver:

Zeman NR. Thermally enhanced bioremediation of LNAPL. [Internet] [Masters thesis]. Colorado State University; 2013. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10217/79208.

Council of Science Editors:

Zeman NR. Thermally enhanced bioremediation of LNAPL. [Masters Thesis]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/79208

University of Arizona

21. Leo, Caitlyn. Toxicity of Arsenic, Uranium, PFOA, and PFOS to Chlorella Sorokiniana and Potential for Bioremeditation Applications .

Degree: 2020, University of Arizona

URL: http://hdl.handle.net/10150/642024

► Off-grid, mobile nanofiltration units are being explored as a method for point-source arsenic and uranium removal from drinking water on Navajo Nation. One proposed treatment… (more)

▼ Off-grid, mobile nanofiltration units are being explored as a method for point-source arsenic and uranium removal from drinking water on Navajo Nation. One proposed treatment method for the concentrated brine treatment method produced during this process is the use of microalgae to remove contaminants via biosorption. Chlorella Sorokiniana was chosen for this study because of its high tolerance for salt and heat as well as previously demonstrated biosorption abilities. This study seeks to establish that C. sorokiniana is a viable species to produce water fit for agricultural use nanofiltration brine by removing of arsenic, uranium, and two species of PFAS. Initial toxicity screenings, growth inhibition experiments, and an analysis of extracellular contaminant concentrations for arsenic, uranium, PFOA, and PFOS were performed to establish a proof of concept. Initial toxicity tests showed arsenic concentrations between 400-500 mg/L caused cell death in 72 hours for C. sorokiniana. From two-week growth inhibition experiments, the species IC50 for arsenic was found to be 184 mg As/L. While growth rate decreased by over 50% at the highest test concentration (200 mg As/L), biomass productivity only decreased by 26%. ICP-MS analysis of the growth media found at higher concentrations C. sorokiniana removed around 10% of arsenic in solution and accumulated 15 mg As/g biomass. When exposed to uranium, C. sorokiniana exhibited cell death after 72 hours at 20 mg/L and an IC50 of 7.04 mg/L. At 10 mg/L there was a 48% decrease in biomass productivity. The highest uranium removal achieved was 87% at an initial concentration of 0.5 mg/L. The adsorption capacity of C. sorokiniana was found to vary between 0.25 mg and 0.40 mg U/ g biomass. Neither arsenic or uranium caused cell death or significant decrease in cell growth at the highest concentrations found in environmental sampling: 0.055 mg As/L and 0.75 mg U/L. However, C. sorokiniana was not able to remove enough of either contaminant to achieve levels below the MCLs. PFOA caused cell death after 72 hours at 3.22 g/L but did not significantly impact C. sorokiniana growth rate or biomass productivity at concentrations up to 100 mg/L. For initial PFOA concentrations of 0.558, 5.58, and 55.8 mg/L, a 74.6%, 71.8%, and a 71.2% reduction in extracellular PFOA was observed. Estimated accumulation per gram biomass ranged from 0.2 – 22 mg PFOA/g biomass and increased with initial PFOA concentration. PFOS did not cause cell death at any tested concentrations, including its solubility limit. Furthermore, it did not impact growth rate or biomass productivity. For initial PFOS concentrations of 0.0485, 0.485, and 4.85 mg/L, a 90.6%, 97.4%, and a 96.6% reduction in extracellular PFOS was observed. Estimated accumulation per gram biomass ranged from 0.02 – 2.7 mg PFOS/g biomass and increased with initial PFOA concentration. The results indicate that there is potential for C. sorokiniana to be used in bioremediation of both PFOS and PFOA. Advisors/Committee Members: Ogden, Kimberly (advisor), Guzman, Roberto (committeemember), Hickenbottom, Kerri (committeemember).

Subjects/Keywords: Algae; Bioremediation; Water treatment

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APA (6^th Edition):

Leo, C. (2020). Toxicity of Arsenic, Uranium, PFOA, and PFOS to Chlorella Sorokiniana and Potential for Bioremeditation Applications . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/642024

Chicago Manual of Style (16^th Edition):

Leo, Caitlyn. “Toxicity of Arsenic, Uranium, PFOA, and PFOS to Chlorella Sorokiniana and Potential for Bioremeditation Applications .” 2020. Masters Thesis, University of Arizona. Accessed March 01, 2021. http://hdl.handle.net/10150/642024.

MLA Handbook (7^th Edition):

Leo, Caitlyn. “Toxicity of Arsenic, Uranium, PFOA, and PFOS to Chlorella Sorokiniana and Potential for Bioremeditation Applications .” 2020. Web. 01 Mar 2021.

Vancouver:

Leo C. Toxicity of Arsenic, Uranium, PFOA, and PFOS to Chlorella Sorokiniana and Potential for Bioremeditation Applications . [Internet] [Masters thesis]. University of Arizona; 2020. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10150/642024.

Council of Science Editors:

Leo C. Toxicity of Arsenic, Uranium, PFOA, and PFOS to Chlorella Sorokiniana and Potential for Bioremeditation Applications . [Masters Thesis]. University of Arizona; 2020. Available from: http://hdl.handle.net/10150/642024

University of Manitoba

22. Krahn, Vanessa. Ethanol enhanced bioremediation of nitrate-contaminated soil and groundwater.

Degree: Biosystems Engineering, 2017, University of Manitoba

URL: http://hdl.handle.net/1993/32553

► Enhanced biodenitrification is a technique for remediation of nutrient impacts that uses electron donor amendments to stimulate the microbial community present in natural groundwater aquifers.… (more)

Subjects/Keywords: Biodenitrification; Bioremediation; Nutrient Impacts

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APA (6^th Edition):

Krahn, V. (2017). Ethanol enhanced bioremediation of nitrate-contaminated soil and groundwater. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/32553

Chicago Manual of Style (16^th Edition):

Krahn, Vanessa. “Ethanol enhanced bioremediation of nitrate-contaminated soil and groundwater.” 2017. Masters Thesis, University of Manitoba. Accessed March 01, 2021. http://hdl.handle.net/1993/32553.

MLA Handbook (7^th Edition):

Krahn, Vanessa. “Ethanol enhanced bioremediation of nitrate-contaminated soil and groundwater.” 2017. Web. 01 Mar 2021.

Vancouver:

Krahn V. Ethanol enhanced bioremediation of nitrate-contaminated soil and groundwater. [Internet] [Masters thesis]. University of Manitoba; 2017. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1993/32553.

Council of Science Editors:

Krahn V. Ethanol enhanced bioremediation of nitrate-contaminated soil and groundwater. [Masters Thesis]. University of Manitoba; 2017. Available from: http://hdl.handle.net/1993/32553

University of Manchester

23. Kimber, Richard. The biogeochemical behaviour of plutonium and americium in contaminated soils.

Degree: PhD, 2012, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347

► The biogeochemical behaviour of plutonium and americium was investigated in contaminated soils from the UK to help determine possible remediation and management options. Stimulating anoxic… (more)

▼ The biogeochemical behaviour of plutonium and americium was investigated in contaminated soils from the UK to help determine possible remediation and management options. Stimulating anoxic sediments from Aldermaston, through the addition of a carbon substrate (glucose), induced reducing conditions resulting in a negligible change in Pu mobility. This was despite a substantial shift in the bacterial profile from a diverse community to one dominated by fermentative Beta proteobacteria and Clostridia. The latter group also includes organisms associated with metal reduction, such as close relatives to Clostridium species, reported previously to facilitate the reduction of Pu(IV) to Pu(III). A sequential extraction was performed on soils from Aldermaston and the Esk Estuary to identify which selected fractions the Pu and Am are most strongly associated with. The majority of Pu was associated with the 'residual fraction': 63.8 – 85.5 % and 91.9 – 94.5 % in the Aldermaston and Esk Estuary soils respectively. Metals associated with this fraction are highly recalcitrant and are unlikely to be released into solution over a significant time span under most geological conditions. The Am was more evenly distributed with the 'organic fraction' being the most dominant. Degradation of organic matter under oxidising conditions may result in mobilization of metals associated with this fraction. The Aldermaston soil was also subjected to bioleaching using a sulfuric acid producing microbial community, which resulted in a maximum 0.18 % of Pu released into solution. However, up to 12.5% of Am was found in solution suggesting Am is more susceptible to mobilization than Pu. The potential for Pu mobilization through abiotic oxidative leaching was investigated using permanganate. Even when carbonate was added to act as a potential complexant for the Pu, less than 1% of the Pu was leached. Greater success was observed when leaching was attempted using citric acid; an estimated 25 – 30% of Pu was released into solution offering a potential route for remediation of Pu-contaminated soils. These data would suggest that the Pu is highly recalcitrant, and may exist in a small particulate form in the Aldermaston soils, possibly in the oxide form, and is unlikely to mobilize under natural biogeochemical conditions.

Subjects/Keywords: 628.5; biogeochemistry; bioremediation; radionuclides; contaminated

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APA (6^th Edition):

Kimber, R. (2012). The biogeochemical behaviour of plutonium and americium in contaminated soils. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347

Chicago Manual of Style (16^th Edition):

Kimber, Richard. “The biogeochemical behaviour of plutonium and americium in contaminated soils.” 2012. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021. https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347.

MLA Handbook (7^th Edition):

Kimber, Richard. “The biogeochemical behaviour of plutonium and americium in contaminated soils.” 2012. Web. 01 Mar 2021.

Vancouver:

Kimber R. The biogeochemical behaviour of plutonium and americium in contaminated soils. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2021 Mar 01]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347.

Council of Science Editors:

Kimber R. The biogeochemical behaviour of plutonium and americium in contaminated soils. [Doctoral Dissertation]. University of Manchester; 2012. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347

Michigan State University

24. Sneathen, Mark Lee. Theoretical and experimental competitiveness of Pseudomonas stutzeri KC.

Degree: MS, Department of Civil and Environmental Engineering, 1996, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:26070

Subjects/Keywords: Pseudomonas; Bioremediation

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APA (6^th Edition):

Sneathen, M. L. (1996). Theoretical and experimental competitiveness of Pseudomonas stutzeri KC. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:26070

Chicago Manual of Style (16^th Edition):

Sneathen, Mark Lee. “Theoretical and experimental competitiveness of Pseudomonas stutzeri KC.” 1996. Masters Thesis, Michigan State University. Accessed March 01, 2021. http://etd.lib.msu.edu/islandora/object/etd:26070.

MLA Handbook (7^th Edition):

Sneathen, Mark Lee. “Theoretical and experimental competitiveness of Pseudomonas stutzeri KC.” 1996. Web. 01 Mar 2021.

Vancouver:

Sneathen ML. Theoretical and experimental competitiveness of Pseudomonas stutzeri KC. [Internet] [Masters thesis]. Michigan State University; 1996. [cited 2021 Mar 01]. Available from: http://etd.lib.msu.edu/islandora/object/etd:26070.

Council of Science Editors:

Sneathen ML. Theoretical and experimental competitiveness of Pseudomonas stutzeri KC. [Masters Thesis]. Michigan State University; 1996. Available from: http://etd.lib.msu.edu/islandora/object/etd:26070

Montana State University

25. Eyre, Julie Ann. Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils.

Degree: MS, College of Engineering, 1999, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/8629

Subjects/Keywords: Biosurfactants.; Bioremediation.

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APA (6^th Edition):

Eyre, J. A. (1999). Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils. (Masters Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/8629

Chicago Manual of Style (16^th Edition):

Eyre, Julie Ann. “Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils.” 1999. Masters Thesis, Montana State University. Accessed March 01, 2021. https://scholarworks.montana.edu/xmlui/handle/1/8629.

MLA Handbook (7^th Edition):

Eyre, Julie Ann. “Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils.” 1999. Web. 01 Mar 2021.

Vancouver:

Eyre JA. Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils. [Internet] [Masters thesis]. Montana State University; 1999. [cited 2021 Mar 01]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8629.

Council of Science Editors:

Eyre JA. Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils. [Masters Thesis]. Montana State University; 1999. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8629

Colorado School of Mines

26. Dangelmayr, Martin A. Biogeochemical controls of uranium remediation and transport.

Degree: PhD, Civil and Environmental Engineering, 2018, Colorado School of Mines

URL: http://hdl.handle.net/11124/172151

► In the U.S. almost 140 sites have been contaminated by uranium mining and milling operations or by the storage of radioactive materials. In-situ recovery (ISR)… (more)

▼ In the U.S. almost 140 sites have been contaminated by uranium mining and milling operations or by the storage of radioactive materials. In-situ recovery (ISR) facilities still face challenges restoring water to pre-mining conditions and leave behind elevated uranium concentrations. Bioremediation and reactive transport modeling are potential tools to mitigate the impact of uranium contamination on human and environmental health, through their ability to immobilize uranium and assess the effectiveness of natural uranium attenuation. This project investigated biogeochemical aspects of both active and natural remediation of uranium contaminated subsurface for two field sites: The Smith Ranch Highland (SRH) site in WY, and the Rifle, Integrated Field Research Challenge (IFRC) site in CO. Our project objectives were to study the transformation of organic substrate during biostimulation and assess uranium retardation due to sorption with sediments taken from an ISR site. This thesis presents two research projects that address the remediation and risk assessment of uranium contaminated sites. The first project evaluated the impact of added organic carbon on the long-term biogeochemical attenuation of uranium in the subsurface of a former mill tailings site. Fluorescence and specific ultraviolet absorption (SUVA) analyses were used together with dissolved organic carbon (DOC) measurements to track organic carbon dynamics during and post-biostimulation of the 2011 Rifle IFRC experiment. An electron mass balance was performed on well CD01 to determine if any carbon sinks were unaccounted for. DOC values increased to 1.76 mM-C during biostimulation, and 3.18 mM-C post-biostimulation over background DOC values of 0.3-0.4 mM-C. Elevated DOC levels persisted 90 days after acetate injections ceased. The electron mass balance revealed that assumed electron acceptors would not account for the total amount of acetate consumed. Fluorescence spectra showed an increase in signals associated with soluble microbial products (SMP), during biostimulation, which disappeared post-biostimulation despite an increase in DOC. SUVA analyses, indicated that DOC present post-biostimulation is less aromatic in nature, compared to background DOC. Our results suggest that microbes convert injected acetate into a carbon sink that may be available to sustain iron reduction post-stimulation The second project consisted of two sets of column experiments and attempted to evaluate the impact of alkalinity and pH on the sorption of uranium in sediments from an ISR site. The ability of thermodynamic models to predict uranium behavior under conditions relevant to ISR restoration sites was also tested. Sediments at three different depths from a monitoring well at the SRH site were used in nine column studies and six batch experiments to study the sorption capacity of SRH sediments and estimate uncertainties associated with fitted parameters. Sediments were characterized by X-Ray Diffraction (XRD) and X-Ray Fluorescence (XRF) for dominant mineralogy and… Advisors/Committee Members: Figueroa, Linda A. (advisor), Stone, James J. (advisor), Sharp, Jonathan O. (committee member), Ranville, James F. (committee member), Bellona, Christopher (committee member).

Subjects/Keywords: groundwater; uranium; sorption; bioremediation

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APA (6^th Edition):

Dangelmayr, M. A. (2018). Biogeochemical controls of uranium remediation and transport. (Doctoral Dissertation). Colorado School of Mines. Retrieved from http://hdl.handle.net/11124/172151

Chicago Manual of Style (16^th Edition):

Dangelmayr, Martin A. “Biogeochemical controls of uranium remediation and transport.” 2018. Doctoral Dissertation, Colorado School of Mines. Accessed March 01, 2021. http://hdl.handle.net/11124/172151.

MLA Handbook (7^th Edition):

Dangelmayr, Martin A. “Biogeochemical controls of uranium remediation and transport.” 2018. Web. 01 Mar 2021.

Vancouver:

Dangelmayr MA. Biogeochemical controls of uranium remediation and transport. [Internet] [Doctoral dissertation]. Colorado School of Mines; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/11124/172151.

Council of Science Editors:

Dangelmayr MA. Biogeochemical controls of uranium remediation and transport. [Doctoral Dissertation]. Colorado School of Mines; 2018. Available from: http://hdl.handle.net/11124/172151

University of Toronto

27. Devine, Cheryl E. Identification of Key Organisms, Genes and Pathways in Benzene-degrading Methanogenic Cultures.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/68915

►

Benzene is a carcinogenic hydrocarbon and a widespread groundwater contaminant. Microbial mineralization of benzene is possible, but the process is slow and not well understood… (more)

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Benzene is a carcinogenic hydrocarbon and a widespread groundwater contaminant. Microbial mineralization of benzene is possible, but the process is slow and not well understood under the anaerobic conditions that are often prevalent at contaminated sites. The goals of this study were to gain a better understanding of key organisms, genes and metabolic pathways associated with benzene degradation in methanogenic enrichment cultures. Conditions required for the successful growth of the cultures were also explored. Potential key organisms were identified via 16S rRNA gene cloning studies, performed using several cultures that had been enriched in parallel from the same hydrocarbon-contaminated site. In all cultures, closely related members of the Syntrophobacterales were found to have survived and thrived over nearly a decade of enrichment. These organisms, referred to as ORM2-like organisms, were phylogenetically most similar to those present in cultures enriched from other benzene-contaminated sites. To link organisms with their potential roles in anaerobic benzene biodegradation, we sequenced, assembled and analyzed the metagenome of one of the benzene-degrading methanogenic cultures. Several assemblies were created from Illumina sequencing data and a combination of these proved to be optimal. An unsupervised binning method was developed to achieve near-complete genomes of the three most abundant phylotypes, including a group of three closely-related ORM2-like species, a hydrogen-utilizing Methanoregula sp., and a previously unidentified organism in the culture, classified as a member of the Candidate Division OD1. The ORM2-like organisms were found to harbor a suite of genes linked to the degradation of the central metabolite benzoyl-CoA. Proteomic data confirmed that these genes were expressed under benzene-degrading conditions, providing strong evidence for the role of ORM2-like organisms as benzene fermenters. Activity of hydrogenotrophic and acetoclastic methanogens was confirmed; other organisms may play peripheral roles that increase the culture robustness. The absence of an easily identified putative anaerobic benzene carboxylase in the ORM2-like genomes suggests a novel mechanism for benzene activation by these organisms. The work presented here provides a valuable blueprint that could be used in identifying biomarkers of anaerobic benzene biodegradation, or in exploring the genomes of organisms from novel genera, orders and even phyla.

PhD

Advisors/Committee Members: Edwards, Elizabeth A., Chemical Engineering and Applied Chemistry.

Subjects/Keywords: anaerobic benzene biodegradation; bioremediation; 0775

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APA (6^th Edition):

Devine, C. E. (2013). Identification of Key Organisms, Genes and Pathways in Benzene-degrading Methanogenic Cultures. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/68915

Chicago Manual of Style (16^th Edition):

Devine, Cheryl E. “Identification of Key Organisms, Genes and Pathways in Benzene-degrading Methanogenic Cultures.” 2013. Doctoral Dissertation, University of Toronto. Accessed March 01, 2021. http://hdl.handle.net/1807/68915.

MLA Handbook (7^th Edition):

Devine, Cheryl E. “Identification of Key Organisms, Genes and Pathways in Benzene-degrading Methanogenic Cultures.” 2013. Web. 01 Mar 2021.

Vancouver:

Devine CE. Identification of Key Organisms, Genes and Pathways in Benzene-degrading Methanogenic Cultures. [Internet] [Doctoral dissertation]. University of Toronto; 2013. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1807/68915.

Council of Science Editors:

Devine CE. Identification of Key Organisms, Genes and Pathways in Benzene-degrading Methanogenic Cultures. [Doctoral Dissertation]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/68915

University of Minnesota

28. Anderson, Emily. Identification of Microorganisms for the Bioremediation of Nitrate and Manganese in Minnesota Water.

Degree: MS, Soil Science, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/208925

► Bioremediation is a way to safely and cost-effectively remove contaminants using living organisms. In this thesis, microorganisms capable of remediating two pollutants, nitrate and manganese,… (more)

▼ Bioremediation is a way to safely and cost-effectively remove contaminants using living organisms. In this thesis, microorganisms capable of remediating two pollutants, nitrate and manganese, were identified using culture-dependent and –independent approaches. Nitrate in agricultural wastewater can lead to algal blooms and eutrophication. Edge-of-field woodchip bioreactors are a promising approach to prevent nitrate in wastewater from reaching surface waters by utilizing microbial denitrification to remove nitrate from the system. However, woodchip bioreactors experience low efficiency under cold temperatures, so one strategy to enhance bioreactors in the early spring involves bioaugmentation, or inoculating the bioreactors with cold-adapted denitrifying microorganisms. In order to identify a cold-adapted denitrifier for bioaugmentation, microorganisms were isolated from field woodchip bioreactors and subjected to denitrification testing under cold temperatures, measuring nitrate, nitrite, ammonium, nitrous oxide and dinitrogen gas, as well as whole genome sequencing to identify the presence of genes involved in denitrification and other important microbial processes. Based off of these results, two strains, Microvirgula sp. BE2.4 and Cellulomonas sp. WB94 were recommended for bioaugmentation. In part two, manganese was addressed. High levels of manganese in drinking water can cause health problems, and common treatment methods require cost-intensive chemicals, conditions and maintenance. In this study, a novel algae bioreactor was established to remove manganese from water. In this bioreactor, the algae provided fixed carbon for manganese-oxidizing microorganisms that oxidized the dissolved manganese, precipitating it out of solution. Using a culture-dependent approach, manganese-oxidizing bacteria and fungi were isolated from an environmental sample, including known oxidizers Bosea, Pseudomonas, Plectosphaerella and Phoma and some not previously known to oxidize manganese such as Aeromonas, Skermanella, Ensifer and Aspergillus. A culture-independent approach was also employed to determine how abundant the isolated manganese-oxidizing bacteria are in an actively oxidizing environmental sample. Using nitrate and manganese as examples, this thesis identified useful microorganisms involved in remediation and demonstrated how microorganisms can be utilized to effectively remove pollutants from the environment.

Subjects/Keywords: bioreactor; bioremediation; denitrification; manganese

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APA (6^th Edition):

Anderson, E. (2018). Identification of Microorganisms for the Bioremediation of Nitrate and Manganese in Minnesota Water. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/208925

Chicago Manual of Style (16^th Edition):

Anderson, Emily. “Identification of Microorganisms for the Bioremediation of Nitrate and Manganese in Minnesota Water.” 2018. Masters Thesis, University of Minnesota. Accessed March 01, 2021. http://hdl.handle.net/11299/208925.

MLA Handbook (7^th Edition):

Anderson, Emily. “Identification of Microorganisms for the Bioremediation of Nitrate and Manganese in Minnesota Water.” 2018. Web. 01 Mar 2021.

Vancouver:

Anderson E. Identification of Microorganisms for the Bioremediation of Nitrate and Manganese in Minnesota Water. [Internet] [Masters thesis]. University of Minnesota; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/11299/208925.

Council of Science Editors:

Anderson E. Identification of Microorganisms for the Bioremediation of Nitrate and Manganese in Minnesota Water. [Masters Thesis]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/208925

Georgia Tech

29. Justicia-Leon, Shandra D. Microbes and monitoring tools for anaerobic chlorinated methane bioremediation.

Degree: PhD, Biology, 2012, Georgia Tech

URL: http://hdl.handle.net/1853/53151

► he chlorinated methanes carbon tetrachloride (CT), chloroform (CF), dichloromethane (DCM) and chloromethane (CM) are widespread groundwater pollutants that pose risks to human and ecosystem health.… (more)

▼ he chlorinated methanes carbon tetrachloride (CT), chloroform (CF), dichloromethane (DCM) and chloromethane (CM) are widespread groundwater pollutants that pose risks to human and ecosystem health. Although some progress has been made in elucidating the microbiology contributing to the aerobic degradation of DCM and CM, these efforts have had little impact on bioremediation practices aimed at restoring anoxic aquifers impacted by chlorinated methanes. Remaining knowledge gaps include the lack of understanding of the microbial mechanisms and pathways contributing to chlorinated methane transformations under anoxic conditions. Thus, the major goals of this research effort were to identify microbes that can contribute to the transformation of chlorinated methanes in the absence of oxygen, and to develop monitoring tools to assess anaerobic chlorinated methane bioremediation at contaminated sites. To accomplish these goals, freshwater and estuarine sediment samples from 45 geographically distinct locations, including 3 sites with reported chlorinated-methane contamination, were collected and screened for CT-, CF-, DCM- and/or CM-degrading activity. DCM degradation was observed in microcosms established with sediment materials from 15 locations, and the sediment-free, DCM-degrading enrichment culture RM was obtained from Rio Mameyes sediment. 16S rRNA-gene based community analysis characterized consortium RM, and identified a Dehalobacter sp. involved in DCM fermentation to non-toxic products. Organism- and process-specific monitoring tools were designed that target the 16S rRNA gene of the DCM-fermenting Dehalobacter sp. and the consortium’s specific 13C-DCM enrichment factor, respectively. Treatability studies using site materials that showed no chlorinated methane degradation activity demonstrated the feasibility of using CF- and DCM-degrading consortia for bioaugmentation applications. Collectively, this study expands our understanding of bacteria contributing to chlorinated methane degradation, provides new tools for monitoring anaerobic DCM degradation, and demonstrates that microbial remedies at chlorinated methane contaminated sites are feasible. Advisors/Committee Members: Löffler, Frank E. (advisor), Mack, E. Erin (committee member), Kubanek, Julia (committee member), Konstantinidis, Konstantinos T. (committee member), Taillefert, Martial (committee member), DiChristina, Thomas J. (committee member).

Subjects/Keywords: Dichloromethane; Chloroform; Bioremediation; Carbon tetrachloride

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Justicia-Leon, S. D. (2012). Microbes and monitoring tools for anaerobic chlorinated methane bioremediation. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/53151

Chicago Manual of Style (16^th Edition):

Justicia-Leon, Shandra D. “Microbes and monitoring tools for anaerobic chlorinated methane bioremediation.” 2012. Doctoral Dissertation, Georgia Tech. Accessed March 01, 2021. http://hdl.handle.net/1853/53151.

MLA Handbook (7^th Edition):

Justicia-Leon, Shandra D. “Microbes and monitoring tools for anaerobic chlorinated methane bioremediation.” 2012. Web. 01 Mar 2021.

Vancouver:

Justicia-Leon SD. Microbes and monitoring tools for anaerobic chlorinated methane bioremediation. [Internet] [Doctoral dissertation]. Georgia Tech; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1853/53151.

Council of Science Editors:

Justicia-Leon SD. Microbes and monitoring tools for anaerobic chlorinated methane bioremediation. [Doctoral Dissertation]. Georgia Tech; 2012. Available from: http://hdl.handle.net/1853/53151

Clemson University

30. Moss, Christopher. MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE.

Degree: MS, Environmental Engineering and Science, 2015, Clemson University

URL: https://tigerprints.clemson.edu/all_theses/2231

► Groundwater contaminants at an industrial site in South Carolina include tetrachloroethene (PCE), trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), vinyl chloride (VC), 1,2,4-trichlorobenzene (1,2,4-TCB), 1,2-dichlorobenzene (1,2-DCB), 1,4-dichlorobenzene… (more)

▼ Groundwater contaminants at an industrial site in South Carolina include tetrachloroethene (PCE), trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), vinyl chloride (VC), 1,2,4-trichlorobenzene (1,2,4-TCB), 1,2-dichlorobenzene (1,2-DCB), 1,4-dichlorobenzene (1,4-DCB), chlorobenzene (CB), and benzene. The source areas at the site include a tank farm area, a grease trap area, the former wastewater lagoons (WWTP) and the former oil retention pond (ORP). A microcosm evaluation was preformed to determine the potential for bioremediation to treat the organic contaminants in the four source areas (within the saturated zone) and in a downgradient portion of the groundwater plume. The objectives were to evaluate 1) anaerobic treatment at the source zones using biostimulation with lactate or emulsified vegetable oil; 2) bioaugmentation with a commercial chloroethene respiring culture; and 3) addition of zero valent iron (ZVI) with lactate. Additional microcosms were prepared using the same conditions and initially incubated anaerobically; these were then converted to aerobic conditions after the chlorinated ethenes were consumed. Controls included anaerobic conditions without amendments, aerobic conditions without amendments, water controls, and autoclaved controls. For the downgradient groundwater microcosms, the same treatments were used except for the two sets with ZVI. Triplicate bottles were prepared for each treatment, resulting in a total of 147 microcosms. Based on observations made during approximately 400 days of monitoring, the following conclusions were reached: Sequential anaerobic/aerobic bioremediation is a viable treatment approach for the WWTP, ORP, and downgradient locations. PCE and TCE can be efficiently dechlorinated to ethene under anaerobic conditions and the aromatic compounds can be oxidized under subsequent aerobic conditions. It is feasible to use only anaerobic biostimulation to remove PCE and TCE for the WWTP and ORP locations, whereas bioaugmentation will be required for the downgradient location. Consideration should also be given to using bioaugmentation for the WWTP and ORP locations, as this will accelerate removal of PCE and TCE so that a switch to aerobic conditions can occur more quickly. There was no compelling evidence in support of anaerobic biodegradation of the aromatic compounds, either via reductive dechlorination to benzene or via anaerobic oxidation. However, aerobic biodegradation of the aromatic compounds is feasible for the WWTP, ORP, and downgradient locations. Development of an aerobic enrichment culture for biodegradation of the aromatic contaminants at the WWTP, ORP and downgradient locations is advisable. Aerobic aromatic degraders are present at the WWTP. Groundwater from this location can be used to develop an indigenous bioaugmentation culture. The process of enrichment can either be done in an on-site reactor or by an off-site vendor. Bioaugmentation with an aerobic enrichment culture will significantly improve the rate of aerobic treatment of the aromatic… Advisors/Committee Members: Freedman, David L, Finneran, Kevin T, Carraway, Elizabeth R.

Subjects/Keywords: bioaugmentation; Bioremediation; chlorobenzene; chloroethene; Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moss, C. (2015). MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/2231

Chicago Manual of Style (16^th Edition):

Moss, Christopher. “MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE.” 2015. Masters Thesis, Clemson University. Accessed March 01, 2021. https://tigerprints.clemson.edu/all_theses/2231.

MLA Handbook (7^th Edition):

Moss, Christopher. “MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE.” 2015. Web. 01 Mar 2021.

Vancouver:

Moss C. MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE. [Internet] [Masters thesis]. Clemson University; 2015. [cited 2021 Mar 01]. Available from: https://tigerprints.clemson.edu/all_theses/2231.

Council of Science Editors:

Moss C. MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE. [Masters Thesis]. Clemson University; 2015. Available from: https://tigerprints.clemson.edu/all_theses/2231

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