subject:(Bioremediation)

Clemson University

1. Haluska, Alex. Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride.

Degree: MS, Environmental Engineering and Earth Sciences, 2014, Clemson University

URL: https://tigerprints.clemson.edu/all_theses/3037

► Chlorinated ethenes are known or suspected carcinogens. They are also among the most frequently detected organic groundwater contaminants because of wide use and poor… (more)

▼ Chlorinated ethenes are known or suspected carcinogens. They are also among the most frequently detected organic groundwater contaminants because of wide use and poor disposal practices. Within anaerobic aquifer systems, Fe(III) reduction is a prevalent terminal electron accepting process and has often been reported as a competitive electron acceptor with respect to trichloroethene (TCE), cis-dichloroethene (cis-DCE), and vinyl chloride (VC) reduction. Often, practitioners add excessive amounts of electron donor to compensate for this competition in an effort to avoid incomplete dechlorination or "cis-DCE and VC stall." Contaminated aquifer material incubations were setup to assess dechlorination rates and extent under Fe(III)-reducing conditions, and to evaluate whether excessive electron donor additions increase either the rate or extent of complete dechlorination of TCE or VC using acetate and vegetable oil-based electron donors. Sediment batch experiments were constructed to specifically assess how Fe(III) speciation and the presence of electron shuttles influenced VC dechlorination. Four treatments were investigated: (1) VC +10 mM ferrihydrite; (2) VC + 10 mM Fe(III)-NTA; (3) VC + 10 mM ferrihydrite + 500 µM AQDS; and (4) VC + 5 mM AQDS. Sediment batch experiments were also set up to specifically evaluate how electron donor concentration affected TCE and VC reduction using acetate and vegetable oils (Newman Zone® Standard without sodium lactate, CAP 18 ME, EOS 598B42) as sole electron donors. Three electron donor strategies were investigated for each series. The first donor strategy was operationally defined as "stoichiometric," in which the electron donor concentration was balanced "electron to electron" with either the TCE or VC present and going to ethene. The second and third approaches were an "excessive" electron donor approach in which 5x and 10x the necessary stoichiometric amount of electron donor was added. The results of the Fe(III) speciation and electron shuttle batch study suggest Fe(III) is not inhibitory to complete dechlorination. In all incubations amended with Fe(III), VC was completely reduced to ethene, but the rate of VC reduction was faster in incubations that were not amended with Fe(III), which suggest that the presence of Fe(III) may slow the rate of reductive dechlorination. The results of the high versus low electron donor batch studies demonstrate that TCE and VC were completely degraded when the vegetable oils were amended at stoichiometric and excessive concentrations. Methane accumulations were similar regardless of the treatment applied. TCE and VC reduction were partially degraded in the presence of acetate, regardless of the concentration. Fe(III) reduction did not inhibit ethene production. The data suggest that the addition of substrate may not be necessary if the supply of indigenous electron donor is sufficient and the appropriate microorganisms are present. If electron donor should be added, amendments can start low and be increased. Advisors/Committee Members: Kevin T Finneran, Committee Chair, Cindy Lee, David Freedman.

Subjects/Keywords: Bioremediation

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APA (6^th Edition):

Haluska, A. (2014). Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/3037

Chicago Manual of Style (16^th Edition):

Haluska, Alex. “Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride.” 2014. Masters Thesis, Clemson University. Accessed February 20, 2020. https://tigerprints.clemson.edu/all_theses/3037.

MLA Handbook (7^th Edition):

Haluska, Alex. “Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride.” 2014. Web. 20 Feb 2020.

Vancouver:

Haluska A. Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride. [Internet] [Masters thesis]. Clemson University; 2014. [cited 2020 Feb 20]. Available from: https://tigerprints.clemson.edu/all_theses/3037.

Council of Science Editors:

Haluska A. Characterizing the Effect of Vegetable Oil-Based Electron Donor Concentration on Complete Dechlorination of Trichloroethene and Vinyl Chloride. [Masters Thesis]. Clemson University; 2014. Available from: https://tigerprints.clemson.edu/all_theses/3037

Oregon State University

2. Li, Jun. Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site.

Degree: MS, Civil Engineering, 2004, Oregon State University

URL: http://hdl.handle.net/1957/29873

Subjects/Keywords: Bioremediation

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APA (6^th Edition):

Li, J. (2004). Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/29873

Chicago Manual of Style (16^th Edition):

Li, Jun. “Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site.” 2004. Masters Thesis, Oregon State University. Accessed February 20, 2020. http://hdl.handle.net/1957/29873.

MLA Handbook (7^th Edition):

Li, Jun. “Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site.” 2004. Web. 20 Feb 2020.

Vancouver:

Li J. Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site. [Internet] [Masters thesis]. Oregon State University; 2004. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/1957/29873.

Council of Science Editors:

Li J. Molecular analysis of bacterial community dynamics during bioaugmentation studies in a soil column and at a field test site. [Masters Thesis]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/29873

Oregon State University

3. Chang, Soon Woong. Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria.

Degree: PhD, Civil Engineering, 1997, Oregon State University

URL: http://hdl.handle.net/1957/33803

► Cometabolic biodegradation processes are potentially useful for the bioremediation of hazardous waste sites. In this study the potential application of phenol-oxidizing and nitrifying bacteria as… (more)

Subjects/Keywords: Bioremediation

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APA (6^th Edition):

Chang, S. W. (1997). Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/33803

Chicago Manual of Style (16^th Edition):

Chang, Soon Woong. “Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria.” 1997. Doctoral Dissertation, Oregon State University. Accessed February 20, 2020. http://hdl.handle.net/1957/33803.

MLA Handbook (7^th Edition):

Chang, Soon Woong. “Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria.” 1997. Web. 20 Feb 2020.

Vancouver:

Chang SW. Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria. [Internet] [Doctoral dissertation]. Oregon State University; 1997. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/1957/33803.

Council of Science Editors:

Chang SW. Cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic ethers by phenol- and ammonia-oxidizing bacteria. [Doctoral Dissertation]. Oregon State University; 1997. Available from: http://hdl.handle.net/1957/33803

University of Waterloo

4. Critchley, Catharine. Stimulating In Situ Denitrification in an Aerobic, Highly Conductive Municipal Drinking Water Aquifer.

Degree: 2010, University of Waterloo

URL: http://hdl.handle.net/10012/5063

► Best or beneficial management practices (BMPs) are often relied upon as a mitigation strategy for nitrate contamination throughout Canada. At a regional scale, reducing the… (more)

▼ Best or beneficial management practices (BMPs) are often relied upon as a mitigation strategy for nitrate contamination throughout Canada. At a regional scale, reducing the quantity of nutrients applied to agricultural land is one BMP approach that has been implemented internationally. While these BMP strategies have been proven to successfully reduce the environmental impact of agriculture on water systems, the time interval between BMP implementation and a noticeable improvement in groundwater quality can be quite extensive. This lag time has been observed at the agriculturally impacted Thornton Well Field in Oxford County. Despite seven years of significant reductions in fertilizer application within the capture zone of this municipal well field, declining nitrate concentrations have yet to be observed in the production water wells. In order to accelerate nitrate reductions at the Thornton Well Field, an integrated approach, combining BMPs with a stimulated in situ denitrification strategy, was implemented. This research focused on the use of a cross-injection scheme to stimulate in situ denitrification within the production aquifer units, up-gradient of the Thornton Well Field. Briefly, this strategy involves injecting a carbon source and electron donor into a high flux aquifer zone using an injection and extraction system positioned perpendicular to the regional flow field. Through altering the geochemical conditions, the injections stimulate indigenous bacteria to reduce harmful nitrate to innocuous dinitrogen gas. The main objectives of this research included: characterizing the hydrogeologic and geochemical properties of the target aquifer; pilot scale testing of the proposed in situ denitrification system; and suggesting an approach for up-scaling to a full-scale treatment scheme capable of remediating the elevated nitrate concentrations at the Thornton Well Field. Core logging, electrical resistivity studies, several methods of hydraulic characterization, tracer testing, and three-dimensional groundwater modelling were used to quantify the physical properties of the target aquifer and to develop a hydrogeologic conceptual model of the site. The aquifer unit was found to be unconfined in the experiment vicinity, consisting of a complex system of six main hydrostratigraphic layers of sand and gravel featuring variable hydraulic conductivity (K) values. Despite the hydrogeologic complexity, the geochemical properties of the aquifer were relatively uniform with depth. Anion, cation, alkalinity, pH, dissolved oxygen, and nitrous oxide data all contributed to this conjecture. Of particular interest, however, were the elevated dissolved oxygen concentrations, which rivalled atmospheric saturation throughout the entire aquifer sequence. The background physical and chemical characterization identified two main challenges that would potentially influence the performance of the in situ denitrification process: stimulating uniform denitrification in the fast flowing, complex aquifer system and overcoming the…

Subjects/Keywords: Bioremediation; Denitrification

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APA (6^th Edition):

Critchley, C. (2010). Stimulating In Situ Denitrification in an Aerobic, Highly Conductive Municipal Drinking Water Aquifer. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/5063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Critchley, Catharine. “Stimulating In Situ Denitrification in an Aerobic, Highly Conductive Municipal Drinking Water Aquifer.” 2010. Thesis, University of Waterloo. Accessed February 20, 2020. http://hdl.handle.net/10012/5063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Critchley, Catharine. “Stimulating In Situ Denitrification in an Aerobic, Highly Conductive Municipal Drinking Water Aquifer.” 2010. Web. 20 Feb 2020.

Vancouver:

Critchley C. Stimulating In Situ Denitrification in an Aerobic, Highly Conductive Municipal Drinking Water Aquifer. [Internet] [Thesis]. University of Waterloo; 2010. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/10012/5063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Critchley C. Stimulating In Situ Denitrification in an Aerobic, Highly Conductive Municipal Drinking Water Aquifer. [Thesis]. University of Waterloo; 2010. Available from: http://hdl.handle.net/10012/5063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

5. Mendoza Sanchez, Itza. Effects of pore-scale velocity and pore-scale physical processes on contaminant biodegradation during transport in groundwater: modeling and experiments.

Degree: 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2047

► Contamination of surface and ground water has emerged as one of the most important environmental issues in developed and developing countries. Bioremediation of groundwater takes… (more)

▼ Contamination of surface and ground water has emerged as one of the most important environmental issues in developed and developing countries. Bioremediation of groundwater takes advantage of bacteria present in the environment to transform toxic compounds to non-toxic metabolites. This biotechnology holds the potential for fast, inexpensive, and effective water decontamination. However, it is still poorly understood and usually not fully controlled due to the lack of information describing the natural phenomena involved. Therefore, a better understanding of the phenomena involved during bioremediation of groundwater could help in the design and implementation of more efficient technologies. The main objective of the present research is to assess how pore-scale physical factors, such as pore-scale velocity, affect the degradation potential of contaminants during transport in groundwater. The target chemicals studied were chlorinated ethenes because they are commonly found in contaminated groundwater sites. To achieve the research objective, the following were employed: a mathematical model that links pore scale processes to the macro-scale representation of contaminant transport; development of numerical tools to solve the mathematical model; and experimental elucidation of the influence of pore-scale flow velocity on the biodegradation of contaminants using column experiments. Results from the mathematical model and experiments were used to elucidate the inter-relationship between physical and biological phenomena at the micro scale. The influence of flow velocity through the porous media (a physical factor) on the biological structure (microbial community in the porous media) was assessed. The results of this investigation contribute to the bioremediation of contaminated groundwater understanding with new insights on the importance of physical transport factors on the biodegradation potential. For example, flow velocity is shown to have an important effect on the degradation potential of chlorinated ethenes. Additionally, the mathematical model and numerical tools have potential application to many other reactive transport problems, including: adsorption onto activated carbon, reaction in packed beds of catalyst, chemical transport in streambeds, and separation in chromatographic columns. Advisors/Committee Members: Autenrieth, Robin L. (advisor), Cunningham, Jeffrey A. (advisor), McGuire, Jennifer T. (committee member), Zhan, Hongbin (committee member).

Subjects/Keywords: bioremediation; column experiments

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APA (6^th Edition):

Mendoza Sanchez, I. (2009). Effects of pore-scale velocity and pore-scale physical processes on contaminant biodegradation during transport in groundwater: modeling and experiments. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2047

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mendoza Sanchez, Itza. “Effects of pore-scale velocity and pore-scale physical processes on contaminant biodegradation during transport in groundwater: modeling and experiments.” 2009. Thesis, Texas A&M University. Accessed February 20, 2020. http://hdl.handle.net/1969.1/ETD-TAMU-2047.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mendoza Sanchez, Itza. “Effects of pore-scale velocity and pore-scale physical processes on contaminant biodegradation during transport in groundwater: modeling and experiments.” 2009. Web. 20 Feb 2020.

Vancouver:

Mendoza Sanchez I. Effects of pore-scale velocity and pore-scale physical processes on contaminant biodegradation during transport in groundwater: modeling and experiments. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2047.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mendoza Sanchez I. Effects of pore-scale velocity and pore-scale physical processes on contaminant biodegradation during transport in groundwater: modeling and experiments. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2047

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rochester Institute of Technology

6. Gallagher, Noreen Anne. Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents.

Degree: MS, Thomas H. Gosnell School of Life Sciences (COS), 2016, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/9296

► Many wastewater treatment plants (WWTPs) are not properly equipped for the removal of various compounds, including non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and hormones. These… (more)

▼ Many wastewater treatment plants (WWTPs) are not properly equipped for the removal of various compounds, including non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and hormones. These compounds are continually discharged into surface waters, which has become an emerging issue for environmental and public health. Microorganisms in the natural environment may play a crucial role in ecosystem self-purification processes such as contaminant degradation. The aim of this research was to determine if there were microorganisms from water and sediment samples located near wastewater effluent outfalls in Central and Western New York capable of degrading ibuprofen, naproxen, acetaminophen, and 17β-estradiol, and if the degradation capability of microorganisms varied by sampling site. An isolation approach was developed using serial enrichment in mineral medium containing each individual pharmaceutical as the sole carbon source available to heterotrophs. After four weeks of enrichment, bacteria were isolated and the growth of each isolate on its selected pharmaceutical source was measured. The biodegradation of pharmaceuticals was then examined with the isolates that showed the most consistent growth. Results from the various enrichment experiments have led to the isolation of several heterotrophic bacteria capable of utilizing the compounds as their sole carbon sources. An isolate cultured from Payne Beach had the ability to remove up to 40.1% ± 3.9% of acetaminophen, 23.2% ± 5.7% of ibuprofen, and 18.6% ± 5.3% of 17β-estradiol and an isolate cultured from Charlotte Beach had the ability to remove up to 23.4% ± 3.5% of ibuprofen, 32.2% ± 2.5% of naproxen, and 29.1% ± 1.9% of 17β-estradiol. The data suggests that there are endogenous heterotrophs located near wastewater outfalls that can degrade various pharmaceuticals, and that the degradation capability of microorganisms on certain compounds may be site specific. Advisors/Committee Members: Jeffrey Lodge, Anna Christina Tyler, Corey Ptak.

Subjects/Keywords: Biodegradation; Bioremediation; Pharmaceuticals

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APA (6^th Edition):

Gallagher, N. A. (2016). Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents. (Masters Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/9296

Chicago Manual of Style (16^th Edition):

Gallagher, Noreen Anne. “Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents.” 2016. Masters Thesis, Rochester Institute of Technology. Accessed February 20, 2020. https://scholarworks.rit.edu/theses/9296.

MLA Handbook (7^th Edition):

Gallagher, Noreen Anne. “Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents.” 2016. Web. 20 Feb 2020.

Vancouver:

Gallagher NA. Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents. [Internet] [Masters thesis]. Rochester Institute of Technology; 2016. [cited 2020 Feb 20]. Available from: https://scholarworks.rit.edu/theses/9296.

Council of Science Editors:

Gallagher NA. Isolation of Pharmaceutical-Degrading Bacteria from Lake Sediments Associated with Wastewater Effluents. [Masters Thesis]. Rochester Institute of Technology; 2016. Available from: https://scholarworks.rit.edu/theses/9296

Montana State University

7. Bowen De Leon, Kara Beth. Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA.

Degree: College of Letters & Science, 2013, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/3475

► Heavy-metal contamination is a common problem in the industrialized world today. Bioremediation, the use of microorganisms or microbial processes to degrade or reduce contaminants, has… (more)

Subjects/Keywords: Bioremediation.; Chromium.; Nitrates.

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APA (6^th Edition):

Bowen De Leon, K. B. (2013). Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/3475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bowen De Leon, Kara Beth. “Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA.” 2013. Thesis, Montana State University. Accessed February 20, 2020. https://scholarworks.montana.edu/xmlui/handle/1/3475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bowen De Leon, Kara Beth. “Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA.” 2013. Web. 20 Feb 2020.

Vancouver:

Bowen De Leon KB. Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA. [Internet] [Thesis]. Montana State University; 2013. [cited 2020 Feb 20]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bowen De Leon KB. Community analysis of groundwater and surrogate sediment samples during electron donor and electron acceptor injections into a chromium-contaminated site in Hanford, Washington, USA. [Thesis]. Montana State University; 2013. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

8. Chang, Chia-Yuan. Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site.

Degree: Master, Environmental Engineering, 2018, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650

► TCE contamination was discovered in 2011 at the research site, and the remediation has been processed since then. After applying KMnO4 for ISCO between 2013… (more)

▼ TCE contamination was discovered in 2011 at the research site, and the remediation has been processed since then. After applying KMnO4 for ISCO between 2013 and 2015, TCE concentration reduced under maximum contaminant level (MCL). However, after stopping injecting substrate, concentration has found bounce back at 4.0 mg/L. To avoid TCE spreading, ground water diffusion control action had been applied between 2009 and 2011 to stopping TCE spreading out of research site. However, the effect to low contamination concentration is limited. Therefore, between 2012 and 2014, two high contamination concentration sites had been selected to apply bioremediation method. Substrate injection can stimulate Dehalococcoides to perform reductive dechlorination of chlorinated ethenes. In that case, concentration will decrease. Objectives of this research included the following (1) Using hydraulic control method to stop contamination from spreading outer research site. (2) Bioremediation: focusing on south of research site at GW1 well to assess long term remediation plan to improve the effective of remediation. (3) Contaminated area monitoring: in conjunction with groundwater contaminated improvement program. Selecting appropriate and existing monitoring wells to exam groundwater quality regularly to develop long term monitoring plan to assess the changes of contamination coverage. The study applied bioremediation method in the entire research site, adopting phased substrate injection with groundwater contamination control. Result on September 2017 showed the TCE contamination concentration were under MCL. GW1 which showed TCE concentration at 0.107 mg/L on February 2016 examine none TCE concentration on September 2017 and last 18 months. Additionally, the changes of contamination coverage had been calculated. Between 2016 and 2017, TCE contamination were lower than 0.05 mg/L. in contamination coverage, TCE contamination volume decreased from 349~702 m3 to 0 m3 indicated the contamination concentration and volume were reduced and under MCL. Consequently, effective of reducing concentration TCE contamination and control contamination coverage were successful in this study. The results indicate that after performing bioremediation process in contaminated source area, the growth of Dehalococcoides can be enhanced, the diffusion of target contaminants in groundwater can be controlled, and the development of bioremediation system to improve the efficiency of TCE reductive dechlorination can be achieved. Advisors/Committee Members: Chih-Ming Kao (committee member), Yao-Ting Tu (chair), Chih-Feng Chen (chair), Chang-Mao Hung (chair).

Subjects/Keywords: Dehalococcoides; bioremediation; TCE

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APA (6^th Edition):

Chang, C. (2018). Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Chia-Yuan. “Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site.” 2018. Thesis, NSYSU. Accessed February 20, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Chia-Yuan. “Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site.” 2018. Web. 20 Feb 2020.

Vancouver:

Chang C. Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site. [Internet] [Thesis]. NSYSU; 2018. [cited 2020 Feb 20]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang C. Remediation of trichloroethylene-contaminated groundwater: Performance evaluation at a case study site. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730118-144650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

9. Heenan, Jeffrey William, 1987-. Geo-electrical monitoring of hydrocarbon degradation.

Degree: PhD, Environmental Science, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61580/

►

The current focus on identifying and remediating contamination calls for further investigation into methods that can be used to efficiently characterize subsurface conditions and monitor… (more)

▼

The current focus on identifying and remediating contamination calls for further investigation into methods that can be used to efficiently characterize subsurface conditions and monitor degradation processes. The primary focus of this research is the latter. Electrical geophysical methods have been well documented as a tool to map aquifers and contaminant plumes, and the same principles that allow for those surveys to be successful allow for these methods to be beneficial for monitoring the progress of remediation. Chemical and physical changes in the subsurface, known to result from biodegradation processes, could be monitored using these methods in situ. This research provides several examples of these methods being effective in monitoring biodegradation. Spectral induced polarization measurements collected over time in a column experiment demonstrate the sensitivity of the method to processes related to microbial induced oil break up, as a result of microbial enhanced oil recovery processes. In another study taking place over the course of approximately 18 months, a progressive decrease in the resistivity of a massive oil spill (Deepwater Horizon) impacted region was observed. Advanced analysis of resistivity variations within the imaged area showed that long-term decreases in resistivity were largely associated with the impacted sediments. Finally, self potential measurements collected at a mature oil spill site, where bioremediation is known to have occurred, showed a measureable, biogeobattery related, response. In all cases, these methods showed the sensitivity of electrical geophysical methods to biodegradation processes. This demonstrates the potential utility of these methods being used an effect monitoring tool to be used in complement with more invasive and expensive chemical/biological testing.

Advisors/Committee Members: Ntarlagiannas, Dimitrios (chair), Slater, Lee (internal member), Atekwana, Estella (outside member), Werkema, Dale (outside member).

Subjects/Keywords: Biogeophyiscs; Biodegradation; Bioremediation

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APA (6^th Edition):

Heenan, Jeffrey William, 1. (2017). Geo-electrical monitoring of hydrocarbon degradation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61580/

Chicago Manual of Style (16^th Edition):

Heenan, Jeffrey William, 1987-. “Geo-electrical monitoring of hydrocarbon degradation.” 2017. Doctoral Dissertation, Rutgers University. Accessed February 20, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/61580/.

MLA Handbook (7^th Edition):

Heenan, Jeffrey William, 1987-. “Geo-electrical monitoring of hydrocarbon degradation.” 2017. Web. 20 Feb 2020.

Vancouver:

Heenan, Jeffrey William 1. Geo-electrical monitoring of hydrocarbon degradation. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2020 Feb 20]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61580/.

Council of Science Editors:

Heenan, Jeffrey William 1. Geo-electrical monitoring of hydrocarbon degradation. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61580/

Virginia Tech

10. Fahrenfeld, Nicole Leah. Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments.

Degree: PhD, Civil Engineering, 2012, Virginia Tech

URL: http://hdl.handle.net/10919/37811

► The nitroaromatic explosive 2,4,6-trinitrotoluene is a widespread, toxic groundwater contaminant. The objective of this work was to describe TNT fate in contaminated aquifer sediments. A… (more)

▼ The nitroaromatic explosive 2,4,6-trinitrotoluene is a widespread, toxic groundwater contaminant. The objective of this work was to describe TNT fate in contaminated aquifer sediments. A series of bench scale experiments and model simulations were performed to evaluate the fate of TNT in historically contaminated aquifer sediments. A TNT contaminated site on the National Priorities List, Former Nansemond Ordnance Depot (FNOD), Suffolk, VA, served as the model site for this work. To describe desorption rate in contaminated sediments, two approaches for a first order single-site desorption were evaluated. In Model 1, the driving force for desorption is mathematically related to the sorbed phase concentrations, whereas in Model 2 the rate is based on aqueous phase concentrations. Two data sets were used to evaluate the models: (1) batch draw-and-fill experiments using FNOD sediment and (2) results from a previously published report from the Louisiana Army Ammunition Plant. Both models provided adequate fit, but Model 2 was better behaved and first order parameters fell within a smaller confidence interval. Draw-and-fill experiments were observed to yield first-order mass transfer coefficients well aligned with those derived from column experiments. The effect of organic amendments on anaerobic TNT degradation rate and microbial community structure in culture enriched from the FNOD site was studied in batch anaerobic microcosms. TNT readily degraded under all experimental conditions. A reductive pathway of TNT degradation was observed across all conditions, however, denaturing gradient gel electrophoresis (DGGE) analysis revealed distinct bacterial community compositions. In all microcosms, Gram-negative γ- or β-Proteobacteria and Gram-positive Negativicutes or Clostridia were observed. According to non-metric multidimensional scaling analysis of DGGE profiles, the microcosm communities were most similar to field site sediment corresponding to the highest TNT concentration, relative to moderately and uncontaminated sediments, suggesting that TNT contamination itself is a major driver of microbial community structure. Candidate degraders were identified and a Pseudomonas sp. was observed to be stimulated under all conditions, which was confirmed to rapidly degrade TNT in pure culture. Mathematical modeling of the batch microcosm results revealed that TNT degraded 1.7 times faster in lactate amended microcosms than in ethanol amended microcosms, which degraded 3.0 times faster than natural organic matter amended microcosms. Simulation of the TNT degradation pathway included determination of branching coefficients representing whether the first reduction of nitro group occurred in the ortho or para position or whether TNT was removed from the aqueous phase (i.e. bound to dissolved organic matter). Branching coefficients were greater for initial reduction of para (17-27% initial TNT concentration) over ortho (3-9% initial TNT concentration) for all test conditions. However, a greater degradate recovery and a different… Advisors/Committee Members: Schreiber, Madeline E. (committee member), Vikesland, Peter J. (committee member), Widdowson, Mark A. (committeecochair), Pruden-Bagchi, Amy Jill (committeecochair).

Subjects/Keywords: sorption; bioremediation; TNT

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APA (6^th Edition):

Fahrenfeld, N. L. (2012). Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/37811

Chicago Manual of Style (16^th Edition):

Fahrenfeld, Nicole Leah. “Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments.” 2012. Doctoral Dissertation, Virginia Tech. Accessed February 20, 2020. http://hdl.handle.net/10919/37811.

MLA Handbook (7^th Edition):

Fahrenfeld, Nicole Leah. “Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments.” 2012. Web. 20 Feb 2020.

Vancouver:

Fahrenfeld NL. Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments. [Internet] [Doctoral dissertation]. Virginia Tech; 2012. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/10919/37811.

Council of Science Editors:

Fahrenfeld NL. Fate of 2,4,6-trinitrotoluene (TNT) in historically contaminated aquifer sediments. [Doctoral Dissertation]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/37811

Indian Institute of Science

11. Ebinesar, J S S Allwin. Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications.

Degree: 2016, Indian Institute of Science

URL: http://hdl.handle.net/2005/3210

► The rivers, lakes and seas are the major water sources for the animal and plant kingdom in this earth. In recent times, the usage and… (more)

▼ The rivers, lakes and seas are the major water sources for the animal and plant kingdom in this earth. In recent times, the usage and wastage of water have been increasing due to the uncontrolled population growth. In addition to that, the rapid industrialization over the years has led to the gradual depletion of the natural resources like water, soil and air. Some of these industries discharge contaminants like organic products and inorganic (or) toxic heavy metals without treatment into the environment, leading to its degradation. Zinc is the 24th most abundant element present in the earth crust, amounting 75 ppm (0.0075%). The concentration of zinc present in the soil and seawater is about 64 ppm and 30 ppb respectively (Emsley, 2001). Generally, the zinc is found with the base metals such as copper and lead and it has less affinity with oxides and strong affinity with sulphides. Sphalerite, a zinc sulphide ore, is majorly containing 60-62% of zinc. The other sources of zinc from the minerals are smithsonite, hemimorphite, quartzite, and hydro zincate. The major sources of zinc contamination arise from several industrial activities such as mining, coal, waste combustion and steel and iron processing. Drinking water also contains certain amounts of Zn, which may be higher when it is stored in metal tanks. The acute toxicity arises from the ingestion of excessive amounts of zinc salts, either accidentally or as dietary supplement. Vomiting, nausea and stomach cramps usually occur after the consumption of more than 500 mg of zinc sulfate. In addition to that, the higher amounts of zinc affect gastrointestinal tract, liver, bone and prostate glands. Finally, Zn can interrupt the activity in soils, as it negatively influences the activity of microorganisms and earthworms, thus retarding the breakdown of organic matter. To combat this problem, techniques such as chemical precipitation, ion exchange, reverse osmosis, etc. are adopted, but these processes result in a huge amount of secondary sludge formation, inefficient removal of metals and are not cost effective. In recent times, an innovative, eco-friendly, cost-effective method has been introduced to treat the toxic heavy metals namely bioremediation. ―Bioremediation‖ is a process of removal of organic or inorganic contaminants by using bacteria, fungi, algae and its metabolites In this research work, the potential of four bacterial strains of the Pseudomonas sp. such as P.putida, P.alcaligenes, P.aeruginosa and P.fluorescens and the extracellular proteins secreted by these four species for the bio-sorption of zinc has been investigated through batch experiments. The mechanisms of interaction between the zinc ion and the bacterial biomass as well as with the extracellular proteins have been elucidated. Additionally, a carbon paste electrode has been modified by using Pseudomonas sp. and its metabolites to develop biosensors for zinc and the lower limit of detection of zinc in aqueous solution has been determined. The major objectives of this research work are… Advisors/Committee Members: Subramanian, S.

Subjects/Keywords: Bioremediation; Zinc Bioremediation; Zinc Bioremediation; Toxic Heavy Metals; Bacterial Cell Structure; In-Situ Bioremediation; Ex-Situ Bioremediation; Pseudomonas Species; Materials Engineering

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APA (6^th Edition):

Ebinesar, J. S. S. A. (2016). Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/3210

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ebinesar, J S S Allwin. “Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications.” 2016. Thesis, Indian Institute of Science. Accessed February 20, 2020. http://hdl.handle.net/2005/3210.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ebinesar, J S S Allwin. “Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications.” 2016. Web. 20 Feb 2020.

Vancouver:

Ebinesar JSSA. Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications. [Internet] [Thesis]. Indian Institute of Science; 2016. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/2005/3210.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ebinesar JSSA. Bioremediation of Zinc using Pseudomonas Species - Mechanistic Studies and Biosensor Applications. [Thesis]. Indian Institute of Science; 2016. Available from: http://hdl.handle.net/2005/3210

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

12. Al-Awadi, Mohammed A. R. Hazard and risk assessment of heavy hydrocarbons undergoing remediation.

Degree: 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166619 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540503

► The focus of this thesis is the bioremediation of oil impacted sites. Chapter 3 considered “Oil Lakes” in Kuwait, a consequence of the First Gulf… (more)

Subjects/Keywords: 577.27; Risk assessment : Hydrocarbons : Bioremediation

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APA (6^th Edition):

Al-Awadi, M. A. R. (2010). Hazard and risk assessment of heavy hydrocarbons undergoing remediation. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166619 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540503

Chicago Manual of Style (16^th Edition):

Al-Awadi, Mohammed A R. “Hazard and risk assessment of heavy hydrocarbons undergoing remediation.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed February 20, 2020. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166619 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540503.

MLA Handbook (7^th Edition):

Al-Awadi, Mohammed A R. “Hazard and risk assessment of heavy hydrocarbons undergoing remediation.” 2010. Web. 20 Feb 2020.

Vancouver:

Al-Awadi MAR. Hazard and risk assessment of heavy hydrocarbons undergoing remediation. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2020 Feb 20]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166619 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540503.

Council of Science Editors:

Al-Awadi MAR. Hazard and risk assessment of heavy hydrocarbons undergoing remediation. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166619 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540503

Rochester Institute of Technology

13. Delp, Danielle. Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds.

Degree: MS, Thomas H. Gosnell School of Life Sciences (COS), 2015, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/8894

► Anthropogenic nutrient pollution is one of the most serious threats facing aquatic systems in modern times. Pollution with compounds containing nitrogen and phosphorous encourages… (more)

▼ Anthropogenic nutrient pollution is one of the most serious threats facing aquatic systems in modern times. Pollution with compounds containing nitrogen and phosphorous encourages rapid development of autotrophic biomass, or blooms, that severely degrade the quality of an aquatic system both as a habitat for organisms and as a water resource for human use. In freshwater ponds, the widespread need for treatment of nutrient pollution and resulting blooms has resulted in the growth of a large industry dedicated to pond management and maintenance. These companies utilize a variety of chemical and non-chemical treatments to prevent the growth of blooms or limit their impact on humans and organisms that use the ponds. This study performed research and development on a new technology that utilizes bioremediation as a means of treating nutrient pollution and autotrophic blooms in freshwater ponds. The agent of bioremediation chosen for use was Chlorella sp. grown as a biofilm in a prototype pond water filtration system. Field trials lasting one year were performed on six freshwater ponds located throughout Central and Western New York to determine if the prototype was capable of reducing the percentage of each pond’s surface that was covered by bloom biomass. The results of the field trials suggest that Chlorella sp. biofilm filtration treatment is a commercially viable option for the treatment of Lemnoideae organisms such as Lemna sp. and Wolffia sp. The concentration of dissolved nitrate, phosphate, and ammonia in each pond was also monitored at three sites; statistically significant changes in the concentration of the target nutrients was observed at two sites via multiple linear regression utilizing percent surface cover as a response. The results of this analysis may allow Chlorella sp. biofilms to be utilized commercially as a bioremediation treatment for nutrient pollution in freshwater ponds. Advisors/Committee Members: Jeffrey Lodge, Karl Korfmacher, Christy Tyler.

Subjects/Keywords: Algae; Biofilm; Bioremediation; Chlorella; Eutrophication

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Delp, D. (2015). Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds. (Masters Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/8894

Chicago Manual of Style (16^th Edition):

Delp, Danielle. “Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds.” 2015. Masters Thesis, Rochester Institute of Technology. Accessed February 20, 2020. https://scholarworks.rit.edu/theses/8894.

MLA Handbook (7^th Edition):

Delp, Danielle. “Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds.” 2015. Web. 20 Feb 2020.

Vancouver:

Delp D. Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds. [Internet] [Masters thesis]. Rochester Institute of Technology; 2015. [cited 2020 Feb 20]. Available from: https://scholarworks.rit.edu/theses/8894.

Council of Science Editors:

Delp D. Developing an algal biofilm filtration system for the remediation of nutrient pollution in freshwater ponds. [Masters Thesis]. Rochester Institute of Technology; 2015. Available from: https://scholarworks.rit.edu/theses/8894

NSYSU

14. Chiou, Yi-shiou. Development of site investigation and remediation strategies at DNAPLâ Case Study.

Degree: Master, Environmental Engineering, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246

► In this study, the site investigation protocol developed by American Society for Testing and Materials (ASTM) was applied to establish a conceptual model and determine… (more)

Subjects/Keywords: ASTM; tetrachloroethylene; bioremediation; risk assessment

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APA (6^th Edition):

Chiou, Y. (2016). Development of site investigation and remediation strategies at DNAPLâ Case Study. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chiou, Yi-shiou. “Development of site investigation and remediation strategies at DNAPLâ Case Study.” 2016. Thesis, NSYSU. Accessed February 20, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chiou, Yi-shiou. “Development of site investigation and remediation strategies at DNAPLâ Case Study.” 2016. Web. 20 Feb 2020.

Vancouver:

Chiou Y. Development of site investigation and remediation strategies at DNAPLâ Case Study. [Internet] [Thesis]. NSYSU; 2016. [cited 2020 Feb 20]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chiou Y. Development of site investigation and remediation strategies at DNAPLâ Case Study. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723116-232246

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Montréal

15. Fournier St-Laurent, Samuel. Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés .

Degree: 2009, Université de Montréal

URL: http://hdl.handle.net/1866/3145

► Le tétrachloroéthène (PCE) et les éthènes chlorés qui lui sont apparentés ont été abondamment utilisés pour plusieurs applications en industrie dès le début du 20e… (more)

▼ Le tétrachloroéthène (PCE) et les éthènes chlorés qui lui sont apparentés ont été abondamment utilisés pour plusieurs applications en industrie dès le début du 20e siècle. Ils sont cependant comptés parmi les polluants les plus communs des sols et de l’eau et beaucoup d’efforts sont déployés afin de les éliminer. Nous croyons que la conversion des éthènes chlorés en éthènes par des microorganismes est une solution prometteuse. Le premier aspect du projet visait donc à établir les conditions pour lesquelles un consortium enrichi en Dehalococcoides ethenogenes permettrait la conversion complète de PCE en éthène. Les expériences réalisées nous ont permis de souligner le rôle de l’acide lactique ajouté aux cultures comme source de carbone et source indirecte d’électrons pour la déhalorespiration. Nous avons également pu établir l’effet de la concentration initiale de biomasse dans les cultures sur le profil de déhalogénation du PCE. Le deuxième aspect du projet visait à développer un protocole d’encapsulation du consortium dans une matrice polymérique afin de profiter des nombreux avantages potentiels de l’encapsulation. Nous avons testé trois montages d’encapsulation différents : atomisation avec jet d’air, atomisation avec vibrations ultrasoniques et « drop-wise ». Le dernier montage prévoyait l’encapsulation des cultures dans des billes d’alginate enrobées de chitosane gélifié par du lignosulfonate. C’est le seul montage qui nous a permis d’encapsuler le consortium de façon efficace sans effet significatifs négatifs sur son activité de déchlorination. Aussi, la comparaison des profils de déhalogénation du PCE de cellules encapsulées et cellules libres a montré une plus faible accumulation de TCE, 1,2-DCE et VC dans les échantillons de cellules encapsulée et, par conséquent, une conversion plus rapide et plus complète du PCE en éthène. Finalement, nous avons observé une tendance favorable à l’idée que les microorganismes encapsulés bénéficient d’un effet de protection contre de faibles concentrations d’oxygène. Advisors/Committee Members: Guiot, Serge (advisor).

Subjects/Keywords: Encapsulation; Biorémédiation; Bioremediation; Alginate; PCE

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APA (6^th Edition):

Fournier St-Laurent, S. (2009). Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés . (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/3145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fournier St-Laurent, Samuel. “Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés .” 2009. Thesis, Université de Montréal. Accessed February 20, 2020. http://hdl.handle.net/1866/3145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fournier St-Laurent, Samuel. “Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés .” 2009. Web. 20 Feb 2020.

Vancouver:

Fournier St-Laurent S. Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés . [Internet] [Thesis]. Université de Montréal; 2009. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/1866/3145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fournier St-Laurent S. Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés . [Thesis]. Université de Montréal; 2009. Available from: http://hdl.handle.net/1866/3145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cape Peninsula University of Technology

16. Santos, Bruno Alexandre Quistorp. Continuous bioremediation of electroplating effluent .

Degree: 2013, Cape Peninsula University of Technology

URL: http://etd.cput.ac.za/handle/20.500.11838/865

► There are significant quantities of free cyanide (F-CN) and heavy metal contaminated effluent being discharged from electroplating operations globally. However, there is an overwhelming tendency… (more)

▼ There are significant quantities of free cyanide (F-CN) and heavy metal contaminated effluent being discharged from electroplating operations globally. However, there is an overwhelming tendency in the industry to use physical and/or chemical treatment methods for cyanides (CNs) and heavy metals in effluent. Although these methods may be effective for certain CNs and heavy metals, they produce toxic by-products and also involve high operational and capital investment costs when compared to bioremediation methods. In this study, the design of a two-stage membrane bioreactor (MBR) system was conceptualised for the bioremediation of CNs and heavy metals in the effluent which was collected from an electroplating facility located in the Western Cape, South Africa. The design included a primary inactive bioremediation stage, to reduce the impact of contaminate concentration fluctuations, and a secondary active bioremediation stage, to remove the residual contaminants, in the effluent under alkaline pH conditions which typify most industrial effluent containing these contaminants. An analysis of the electroplating effluent revealed that the effluent contained an average of 149.11 (± 9.31) mg/L, 5.25 (± 0.64) mg/L, 8.12 (± 4.78) mg/L, 9.05 (± 5.26) mg/L and 45.19 (± 25.89) mg/L of total cyanide (T-CN), F-CN, weak acid dissociable cyanides (WAD-CNs), nickel (Ni), zinc (Zn) and copper (Cu), respectively. An Aspergillus sp., which displayed the characteristic black conidiophores of the Aspergillus section Nigri, was isolated from the electroplating facilities’ effluent discharge using a selective pectin agar (PA) and subcultured on 2% (v/v) antibiotic (10,000 units/L penicillin and 10 mg streptomycin/mL) potato dextrose agar (PDA). The isolate was tolerant to F-CN up to 430 mg F-CN/L on F-CN PDA plates which were incubated at 37 ˚C for 5 days. However, a significant decline in microbial growth was observed after 200 mg F-CN/L, thus indicating that the isolate was suitable for the bioremediation of the electroplating effluent. The identification of the isolate as Aspergillus awamori (A. awamori) was definitively determined using a multi-gene phylogenetic analysis, utilising ITS (internal transcribed spacer), -tubulin and calmodulin gene regions. Although an anomaly in the morphology of the conidia of the isolate was observed during the morphological analysis, indicating a possible morphological mutation in the isolate. A comparative study between “sweet orange” (Citrus sinensis (C. sinensis)) pomace, “apple” (Malus domestica (M. domestica)) pomace, “sweetcorn” (Zea mays (Z. mays)) cob and “potato” (Solanum tuberosum (S. tuberosum)) peel, i.e. waste materials considered to be agricultural residues, was conducted in order to assess their potential and as a sole carbon source supplement for A. awamori biomass development for the bioremediation of CNs and heavy metals. The suitability of these agricultural residues for these activities were as follows: C. sinensis pomace ˃ M. domestica pomace ˃ Z. mays cob ˃ S. tuberosum peel. For…

Subjects/Keywords: Bioremediation; Electroplating; Dissertations, Academic; MTech

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APA (6^th Edition):

Santos, B. A. Q. (2013). Continuous bioremediation of electroplating effluent . (Thesis). Cape Peninsula University of Technology. Retrieved from http://etd.cput.ac.za/handle/20.500.11838/865

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santos, Bruno Alexandre Quistorp. “Continuous bioremediation of electroplating effluent .” 2013. Thesis, Cape Peninsula University of Technology. Accessed February 20, 2020. http://etd.cput.ac.za/handle/20.500.11838/865.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santos, Bruno Alexandre Quistorp. “Continuous bioremediation of electroplating effluent .” 2013. Web. 20 Feb 2020.

Vancouver:

Santos BAQ. Continuous bioremediation of electroplating effluent . [Internet] [Thesis]. Cape Peninsula University of Technology; 2013. [cited 2020 Feb 20]. Available from: http://etd.cput.ac.za/handle/20.500.11838/865.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santos BAQ. Continuous bioremediation of electroplating effluent . [Thesis]. Cape Peninsula University of Technology; 2013. Available from: http://etd.cput.ac.za/handle/20.500.11838/865

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Miami University

17. Hwang, Chiachi. Assessment of bacterial communities and an iron-reducing bacterium in relation to an engineered bioremediation system designed for the treatment of uranium-nitric acid contaminated groundwater.

Degree: PhD, Microbiology, 2009, Miami University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=miami1241117969

► The elucidation of how populations of interest interact in a given community and how the community responds to stress and perturbations can provide insight into… (more)

▼ The elucidation of how populations of interest interact in a given community and how the community responds to stress and perturbations can provide insight into the interplay between stress pathways and gene networks that help optimize bacterial biochemistry. The goal of the present study was to characterize the responses of bacterial communities at multiple levels of resolution to understand microbial biochemical capacity at DOE waste sites. The field work at the Field Research Center of the U.S. Department of Energy, Oak Ridge, TN, used a field scale denitrifying fluidized bed reactor (FBR) for nitrate treatment of the groundwater and a series of wells to stimulate microbial growth via ethanol for in situ U(VI) immobilization. Bacterial community dynamics were investigated during the initial start-up of the FBR while those from the groundwater of the wells were studied over a 1.5-yr period. In addition, the physiology and the genome of the isolate, Anaeromyxobacter Fw109-5, from the site were studied to examine its potential role in U(VI) remediation. The subsurface environment was altered via engineering controls during successive phases to better understand strategies that would improve the remediation process. Within this framework, the interrelationship of bacterial communities and geochemistry was studied at different spatial and temporal scales to characterize the ecosystem ecology of an engineered system. Bacterial communities from both FBR and groundwater samples were analyzed via clone libraries of partial SSU rRNA genes. Multivariate analyses were applied to correlate the changes in the bacterial communities to the measured physicochemical parameters. Our results from the field experiments indicated that there was an important interaction between the engineering controls that altered the subsurface geochemistry over time that influenced bacterial population responses. Growth study experiments and genomic analysis also revealed insights to the physiological potential of an iron-reducing isolate, Anaeromyxobacter Fw109-5. The strong associations between particular environmental variables and certain population distributions will provide insights into establishing practical and successful remediation strategies in radionuclide-contaminated environments with respect to engineering controls. Advisors/Committee Members: Janssen, Gary (Committee Chair), Fields, Matthew (Advisor).

Subjects/Keywords: Microbiology; bacterial ecology; uranium; bioremediation

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APA (6^th Edition):

Hwang, C. (2009). Assessment of bacterial communities and an iron-reducing bacterium in relation to an engineered bioremediation system designed for the treatment of uranium-nitric acid contaminated groundwater. (Doctoral Dissertation). Miami University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=miami1241117969

Chicago Manual of Style (16^th Edition):

Hwang, Chiachi. “Assessment of bacterial communities and an iron-reducing bacterium in relation to an engineered bioremediation system designed for the treatment of uranium-nitric acid contaminated groundwater.” 2009. Doctoral Dissertation, Miami University. Accessed February 20, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=miami1241117969.

MLA Handbook (7^th Edition):

Hwang, Chiachi. “Assessment of bacterial communities and an iron-reducing bacterium in relation to an engineered bioremediation system designed for the treatment of uranium-nitric acid contaminated groundwater.” 2009. Web. 20 Feb 2020.

Vancouver:

Hwang C. Assessment of bacterial communities and an iron-reducing bacterium in relation to an engineered bioremediation system designed for the treatment of uranium-nitric acid contaminated groundwater. [Internet] [Doctoral dissertation]. Miami University; 2009. [cited 2020 Feb 20]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1241117969.

Council of Science Editors:

Hwang C. Assessment of bacterial communities and an iron-reducing bacterium in relation to an engineered bioremediation system designed for the treatment of uranium-nitric acid contaminated groundwater. [Doctoral Dissertation]. Miami University; 2009. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami1241117969

University of Manchester

18. Kimber, Richard. The biogeochemical behaviour of plutonium and americium in contaminated soils.

Degree: PhD, 2012, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347

► The biogeochemical behaviour of plutonium and americium was investigated in contaminated soils from the UK to help determine possible remediation and management options. Stimulating anoxic… (more)

▼ The biogeochemical behaviour of plutonium and americium was investigated in contaminated soils from the UK to help determine possible remediation and management options. Stimulating anoxic sediments from Aldermaston, through the addition of a carbon substrate (glucose), induced reducing conditions resulting in a negligible change in Pu mobility. This was despite a substantial shift in the bacterial profile from a diverse community to one dominated by fermentative Beta proteobacteria and Clostridia. The latter group also includes organisms associated with metal reduction, such as close relatives to Clostridium species, reported previously to facilitate the reduction of Pu(IV) to Pu(III). A sequential extraction was performed on soils from Aldermaston and the Esk Estuary to identify which selected fractions the Pu and Am are most strongly associated with. The majority of Pu was associated with the 'residual fraction': 63.8 – 85.5 % and 91.9 – 94.5 % in the Aldermaston and Esk Estuary soils respectively. Metals associated with this fraction are highly recalcitrant and are unlikely to be released into solution over a significant time span under most geological conditions. The Am was more evenly distributed with the 'organic fraction' being the most dominant. Degradation of organic matter under oxidising conditions may result in mobilization of metals associated with this fraction. The Aldermaston soil was also subjected to bioleaching using a sulfuric acid producing microbial community, which resulted in a maximum 0.18 % of Pu released into solution. However, up to 12.5% of Am was found in solution suggesting Am is more susceptible to mobilization than Pu. The potential for Pu mobilization through abiotic oxidative leaching was investigated using permanganate. Even when carbonate was added to act as a potential complexant for the Pu, less than 1% of the Pu was leached. Greater success was observed when leaching was attempted using citric acid; an estimated 25 – 30% of Pu was released into solution offering a potential route for remediation of Pu-contaminated soils. These data would suggest that the Pu is highly recalcitrant, and may exist in a small particulate form in the Aldermaston soils, possibly in the oxide form, and is unlikely to mobilize under natural biogeochemical conditions.

Subjects/Keywords: 628.5; biogeochemistry; bioremediation; radionuclides; contaminated

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APA (6^th Edition):

Kimber, R. (2012). The biogeochemical behaviour of plutonium and americium in contaminated soils. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347

Chicago Manual of Style (16^th Edition):

Kimber, Richard. “The biogeochemical behaviour of plutonium and americium in contaminated soils.” 2012. Doctoral Dissertation, University of Manchester. Accessed February 20, 2020. https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347.

MLA Handbook (7^th Edition):

Kimber, Richard. “The biogeochemical behaviour of plutonium and americium in contaminated soils.” 2012. Web. 20 Feb 2020.

Vancouver:

Kimber R. The biogeochemical behaviour of plutonium and americium in contaminated soils. [Internet] [Doctoral dissertation]. University of Manchester; 2012. [cited 2020 Feb 20]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347.

Council of Science Editors:

Kimber R. The biogeochemical behaviour of plutonium and americium in contaminated soils. [Doctoral Dissertation]. University of Manchester; 2012. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-biogeochemical-behaviour-of-plutonium-and-americium-in-contaminated-soils(d563c675-11aa-4dff-bca5-558c0990958f).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564347

Louisiana State University

19. Fitch, LeeAnn Renee. Biodegradation of Buried MC252 Oil in Coastal Beach Sands by PAH Degraders in Response to Oxygen Biostimulation.

Degree: MS, Civil and Environmental Engineering, 2016, Louisiana State University

URL: etd-07052016-105439 ; https://digitalcommons.lsu.edu/gradschool_theses/114

► Six years following the Deepwater Horizon oil spill, buried MC252 oil persists on a beach segment in Fourchon, LA due to the natural anaerobic conditions… (more)

Subjects/Keywords: oil; bioremediation; PAH degraders

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APA (6^th Edition):

Fitch, L. R. (2016). Biodegradation of Buried MC252 Oil in Coastal Beach Sands by PAH Degraders in Response to Oxygen Biostimulation. (Masters Thesis). Louisiana State University. Retrieved from etd-07052016-105439 ; https://digitalcommons.lsu.edu/gradschool_theses/114

Chicago Manual of Style (16^th Edition):

Fitch, LeeAnn Renee. “Biodegradation of Buried MC252 Oil in Coastal Beach Sands by PAH Degraders in Response to Oxygen Biostimulation.” 2016. Masters Thesis, Louisiana State University. Accessed February 20, 2020. etd-07052016-105439 ; https://digitalcommons.lsu.edu/gradschool_theses/114.

MLA Handbook (7^th Edition):

Fitch, LeeAnn Renee. “Biodegradation of Buried MC252 Oil in Coastal Beach Sands by PAH Degraders in Response to Oxygen Biostimulation.” 2016. Web. 20 Feb 2020.

Vancouver:

Fitch LR. Biodegradation of Buried MC252 Oil in Coastal Beach Sands by PAH Degraders in Response to Oxygen Biostimulation. [Internet] [Masters thesis]. Louisiana State University; 2016. [cited 2020 Feb 20]. Available from: etd-07052016-105439 ; https://digitalcommons.lsu.edu/gradschool_theses/114.

Council of Science Editors:

Fitch LR. Biodegradation of Buried MC252 Oil in Coastal Beach Sands by PAH Degraders in Response to Oxygen Biostimulation. [Masters Thesis]. Louisiana State University; 2016. Available from: etd-07052016-105439 ; https://digitalcommons.lsu.edu/gradschool_theses/114

Montana State University

20. Schultz, Logan Nicholas. Reactive transport in biofouled and biomineralized porous media.

Degree: College of Engineering, 2010, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/2216

► The geologic subsurface environment contains regions of high porosity where fluid flows both naturally and during engineered technologies such as carbon sequestration, enhanced oil recovery,… (more)

▼ The geologic subsurface environment contains regions of high porosity where fluid flows both naturally and during engineered technologies such as carbon sequestration, enhanced oil recovery, and bioremediation of contaminants. In these porous media regions, microbes can have both desirable and undesirable effects on the hydrodynamics and fluid chemistry by inducing the formation of microbial aggregates, which can include extracellular polymeric substance and abiotic particles such as mineral precipitates. While not the focus of this research, these analyses are likewise applicable to biomatter control scenarios in filtration systems and other industrial reactors with a high surface area to volume ratio. Microbially-induced ureolytic calcium carbonate precipitation has been suggested as a means to mitigate leakage from geologic CO 2 sequestration sites and as a means to immobilize divalent contaminants such as strontium-90 in remediation scenarios. In this process, microbes hydrolyze urea, increasing the solution pH, generating carbonate ions, and ultimately shifting the saturation state of the fluid and leading to solid calcium carbonate (CaCO 3) formation in a calcium-rich environment. Experiments were conducted to assess the distribution and effects of biofouling and biomineralization in two-dimensional flat plate reactors with 1mm pore spaces simulating a tortuous porous media environment. In biomineralization experiments, calcium carbonate was formed under flow conditions, and strontium was effectively immobilized within the crystal lattice, suggesting the applicability of subsurface biotechnical applications utilizing this technology. Image, residence time distribution, and piezometer analyses of biofouling experiments quantified porosity and hydraulic conductivity reductions. Biofilms were grown under constant flow and head conditions and were shown to be more channeled and evenly distributed along the flow path in constant flow conditions. Biofilms were challenged with chlorinated bleach, which temporarily increased the hydraulic conductivity, yet failed to remove significant biofouling unless coupled with significant fluid shear. In situ methods utilizing stereo and confocal microscopy were developed to visualize and quantify the distribution of biomatter formation and analyze the biological environment at the surface of bio-induced minerals. Advisors/Committee Members: Chairperson, Graduate Committee: Robin Gerlach. (advisor).

Subjects/Keywords: Biomineralization.; Porous materials.; Biofilms.; Bioremediation.

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APA (6^th Edition):

Schultz, L. N. (2010). Reactive transport in biofouled and biomineralized porous media. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/2216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schultz, Logan Nicholas. “Reactive transport in biofouled and biomineralized porous media.” 2010. Thesis, Montana State University. Accessed February 20, 2020. https://scholarworks.montana.edu/xmlui/handle/1/2216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schultz, Logan Nicholas. “Reactive transport in biofouled and biomineralized porous media.” 2010. Web. 20 Feb 2020.

Vancouver:

Schultz LN. Reactive transport in biofouled and biomineralized porous media. [Internet] [Thesis]. Montana State University; 2010. [cited 2020 Feb 20]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schultz LN. Reactive transport in biofouled and biomineralized porous media. [Thesis]. Montana State University; 2010. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

21. VanEngelen, Catherine Elizabeth. Alkaline hydrolysis of explosives.

Degree: College of Engineering, 2010, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/2465

► In the United States, ammunitions testing and manufacturing facilities must transform unused explosives into non-hazardous materials for disposal. 2,4,6- trinitrotoluene (TNT) is an explosive that… (more)

▼ In the United States, ammunitions testing and manufacturing facilities must transform unused explosives into non-hazardous materials for disposal. 2,4,6- trinitrotoluene (TNT) is an explosive that has been found as a soil and groundwater contaminant at numerous ammunitions testing sites. Unused quantities of nitrocellulose (NC), another explosive, have also been accumulating at ammunitions manufacturing facilities. Transformation of both TNT and NC to non-explosive compounds has been studied using either chemical or biological approaches, each with limited success. With respect to TNT, the use of alkaline hydrolysis (degradation at high pH) as a chemical treatment had been tested at room temperature (20°C) under conditions where the hydroxide concentration exceeded that of TNT (pH > 10). These high hydroxide conditions were not directly amenable to biological treatment of the hydrolysis products. This study found that alkaline hydrolysis was effective for complete degradation of TNT at elevated temperatures (60°C and 80°C) when the concentration of TNT was less than the hydroxide concentration (pH 9 and 10). The resulting solution, or hydrolysate, contained no TNT. This hydrolysate was used as the carbon and nitrogen source for an aerobic bacterial enrichment from the Bozeman wastewater treatment plant. With respect to NC, the back-log of accumulated NC necessitates a degradation method that will process high NC concentrations (200g/L). Alkaline hydrolysis at 60°C was used with very high hydroxide concentrations to rapidly degrade high concentrations of NC, producing high nitrate and nitrite concentrations. The NC hydrolysate was neutralized and spiked into a denitrifying culture which was able to reduce both nitrate and nitrite. The goal of this work was to develop a dual component chemical-biological system for complete degradation of the explosives TNT and NC, which was achieved using alkaline hydrolysis as the chemical component and bacterial wastewater treatment enrichments as the biological component. Advisors/Committee Members: Chairperson, Graduate Committee: Brent M. Peyton. (advisor).

Subjects/Keywords: Hydrolysis.; Bioremediation.; TNT (Chemical).; Nitrocellulose.

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APA (6^th Edition):

VanEngelen, C. E. (2010). Alkaline hydrolysis of explosives. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/2465

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

VanEngelen, Catherine Elizabeth. “Alkaline hydrolysis of explosives.” 2010. Thesis, Montana State University. Accessed February 20, 2020. https://scholarworks.montana.edu/xmlui/handle/1/2465.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

VanEngelen, Catherine Elizabeth. “Alkaline hydrolysis of explosives.” 2010. Web. 20 Feb 2020.

Vancouver:

VanEngelen CE. Alkaline hydrolysis of explosives. [Internet] [Thesis]. Montana State University; 2010. [cited 2020 Feb 20]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2465.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

VanEngelen CE. Alkaline hydrolysis of explosives. [Thesis]. Montana State University; 2010. Available from: https://scholarworks.montana.edu/xmlui/handle/1/2465

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

22. Eyre, Julie Ann. Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils.

Degree: College of Engineering, 1999, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/8629

Subjects/Keywords: Biosurfactants.; Bioremediation.

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APA (6^th Edition):

Eyre, J. A. (1999). Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/8629

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Eyre, Julie Ann. “Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils.” 1999. Thesis, Montana State University. Accessed February 20, 2020. https://scholarworks.montana.edu/xmlui/handle/1/8629.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Eyre, Julie Ann. “Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils.” 1999. Web. 20 Feb 2020.

Vancouver:

Eyre JA. Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils. [Internet] [Thesis]. Montana State University; 1999. [cited 2020 Feb 20]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8629.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Eyre JA. Influence of biosurfactant and non-biosurfactant producing bacteria on phenanthrene removal from model soils. [Thesis]. Montana State University; 1999. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8629

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Clemson University

23. Moss, Christopher. MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE.

Degree: MS, Environmental Engineering and Science, 2015, Clemson University

URL: https://tigerprints.clemson.edu/all_theses/2231

► Groundwater contaminants at an industrial site in South Carolina include tetrachloroethene (PCE), trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), vinyl chloride (VC), 1,2,4-trichlorobenzene (1,2,4-TCB), 1,2-dichlorobenzene (1,2-DCB), 1,4-dichlorobenzene… (more)

▼ Groundwater contaminants at an industrial site in South Carolina include tetrachloroethene (PCE), trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), vinyl chloride (VC), 1,2,4-trichlorobenzene (1,2,4-TCB), 1,2-dichlorobenzene (1,2-DCB), 1,4-dichlorobenzene (1,4-DCB), chlorobenzene (CB), and benzene. The source areas at the site include a tank farm area, a grease trap area, the former wastewater lagoons (WWTP) and the former oil retention pond (ORP). A microcosm evaluation was preformed to determine the potential for bioremediation to treat the organic contaminants in the four source areas (within the saturated zone) and in a downgradient portion of the groundwater plume. The objectives were to evaluate 1) anaerobic treatment at the source zones using biostimulation with lactate or emulsified vegetable oil; 2) bioaugmentation with a commercial chloroethene respiring culture; and 3) addition of zero valent iron (ZVI) with lactate. Additional microcosms were prepared using the same conditions and initially incubated anaerobically; these were then converted to aerobic conditions after the chlorinated ethenes were consumed. Controls included anaerobic conditions without amendments, aerobic conditions without amendments, water controls, and autoclaved controls. For the downgradient groundwater microcosms, the same treatments were used except for the two sets with ZVI. Triplicate bottles were prepared for each treatment, resulting in a total of 147 microcosms. Based on observations made during approximately 400 days of monitoring, the following conclusions were reached: Sequential anaerobic/aerobic bioremediation is a viable treatment approach for the WWTP, ORP, and downgradient locations. PCE and TCE can be efficiently dechlorinated to ethene under anaerobic conditions and the aromatic compounds can be oxidized under subsequent aerobic conditions. It is feasible to use only anaerobic biostimulation to remove PCE and TCE for the WWTP and ORP locations, whereas bioaugmentation will be required for the downgradient location. Consideration should also be given to using bioaugmentation for the WWTP and ORP locations, as this will accelerate removal of PCE and TCE so that a switch to aerobic conditions can occur more quickly. There was no compelling evidence in support of anaerobic biodegradation of the aromatic compounds, either via reductive dechlorination to benzene or via anaerobic oxidation. However, aerobic biodegradation of the aromatic compounds is feasible for the WWTP, ORP, and downgradient locations. Development of an aerobic enrichment culture for biodegradation of the aromatic contaminants at the WWTP, ORP and downgradient locations is advisable. Aerobic aromatic degraders are present at the WWTP. Groundwater from this location can be used to develop an indigenous bioaugmentation culture. The process of enrichment can either be done in an on-site reactor or by an off-site vendor. Bioaugmentation with an aerobic enrichment culture will significantly improve the rate of aerobic treatment of the aromatic… Advisors/Committee Members: Freedman, David L, Finneran, Kevin T, Carraway, Elizabeth R.

Subjects/Keywords: bioaugmentation; Bioremediation; chlorobenzene; chloroethene; Engineering

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APA (6^th Edition):

Moss, C. (2015). MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/2231

Chicago Manual of Style (16^th Edition):

Moss, Christopher. “MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE.” 2015. Masters Thesis, Clemson University. Accessed February 20, 2020. https://tigerprints.clemson.edu/all_theses/2231.

MLA Handbook (7^th Edition):

Moss, Christopher. “MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE.” 2015. Web. 20 Feb 2020.

Vancouver:

Moss C. MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE. [Internet] [Masters thesis]. Clemson University; 2015. [cited 2020 Feb 20]. Available from: https://tigerprints.clemson.edu/all_theses/2231.

Council of Science Editors:

Moss C. MICROCOSM EVALUATION OF ENHANCED BIOREMEDIATION FOR AN INDUSTRIAL SITE CONTAMINATED WITH CHLORINATED ETHENES, CHLORINATED BENZENES, AND BENZENE. [Masters Thesis]. Clemson University; 2015. Available from: https://tigerprints.clemson.edu/all_theses/2231

Oregon State University

24. Ennis, Elliot G. Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater.

Degree: MS, Chemistry, 2005, Oregon State University

URL: http://hdl.handle.net/1957/22656

► Chlorinated aliphatic hydrocarbons (CAHs) such as vinyl chloride (VC) and 1,-2 dichioroethane (DCA), are wide-spread groundwater pollutants found at many contaminated field sites around the… (more)

Subjects/Keywords: Bioremediation – Evaluation

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APA (6^th Edition):

Ennis, E. G. (2005). Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/22656

Chicago Manual of Style (16^th Edition):

Ennis, Elliot G. “Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater.” 2005. Masters Thesis, Oregon State University. Accessed February 20, 2020. http://hdl.handle.net/1957/22656.

MLA Handbook (7^th Edition):

Ennis, Elliot G. “Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater.” 2005. Web. 20 Feb 2020.

Vancouver:

Ennis EG. Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater. [Internet] [Masters thesis]. Oregon State University; 2005. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/1957/22656.

Council of Science Editors:

Ennis EG. Fluorinated and deuterated surrogates for quantifying microbial transformation of chlorinated ethenes and ethanes in anaerobic groundwater. [Masters Thesis]. Oregon State University; 2005. Available from: http://hdl.handle.net/1957/22656

University of Connecticut

25. Zhang, Xiaolong. Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil.

Degree: MS, Environmental Engineering, 2011, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/40

► Hexavalent chromium (Cr(VI)) has been identified as a high priority pollutant by the U.S. Environmental Protection Agency because of its high toxicity and carcinogenicity.… (more)

▼ Hexavalent chromium (Cr(VI)) has been identified as a high priority pollutant by the U.S. Environmental Protection Agency because of its high toxicity and carcinogenicity. Cr(VI) is commonly used in electroplating, pigment, leather processing and mining resulting in widespread Cr(VI) pollution, and a need for the development of cost-effective remediation technologies. Microbial reduction of Cr(VI) to non-toxic trivalent chromium (Cr(III)) is a promising technique. In this study, five bacteria were isolated from the soil of Cr(VI) contaminated plating facility in Putnam, CT. Then were identified tentatively as Leucobacter sp., Serratia sp., Achromobacter sp., Delftia sp. and Pseudomonas fluorescens using the 16S rRNA technique. The biological reduction of aqueous Cr(VI) was initially evaluated in mixed culture of the five bacteria. pH, temperature and initial Cr(VI) concentration had a significant effect on Cr(VI) reduction. The highest Cr(VI) reduction rate was obtained at pH=8, 25-30°C and an initial Cr(VI) concentration less than 50mg/L. Approximately 98% of 40 mg/L Cr(VI) was reduced within 48 hours culture at these conditions. Cr(VI) reduction was shown to correlate with bacterial growth. Bio-adsorption experiment confirmed that the decrease in Cr(VI) not due to bio-adsorption, and bio-reduction was proved by further chromium analysis. Scanning Electron Microscopy was also employed to observe the morphology of the bacteria in cultures with and without Cr(VI). Finally, Pseudomonas fluorescens was used to evaluate Cr(VI) reduction in sterilized and live soil samples. When added at a dosage of 30 mg Cr(VI) per kg dry soil, all Cr(VI) was reduced within one day of curing without the addition of an external carbon source. At an addition of 500 mg/kg Cr(VI), 60% of Cr(VI) was reduced in the sterilized soil within 5 days with 10% (w/w) peptone added, while around 22% Cr(VI) reduction was achieved in the sterilized soil without peptone added. Compared to the sterilized soil, the Cr(VI) reduction rate was slightly increased in the live soil with peptone added. Advisors/Committee Members: Baikun Li;Jose Amador, Maria Chrysochoou.

Subjects/Keywords: hexavalent chromium; aerobic bacteria; bioremediation

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APA (6^th Edition):

Zhang, X. (2011). Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/40

Chicago Manual of Style (16^th Edition):

Zhang, Xiaolong. “Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil.” 2011. Masters Thesis, University of Connecticut. Accessed February 20, 2020. https://opencommons.uconn.edu/gs_theses/40.

MLA Handbook (7^th Edition):

Zhang, Xiaolong. “Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil.” 2011. Web. 20 Feb 2020.

Vancouver:

Zhang X. Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil. [Internet] [Masters thesis]. University of Connecticut; 2011. [cited 2020 Feb 20]. Available from: https://opencommons.uconn.edu/gs_theses/40.

Council of Science Editors:

Zhang X. Investigation of Hexavalent Chromium Reduction by Aerobic Bacteria in Water and Soil. [Masters Thesis]. University of Connecticut; 2011. Available from: https://opencommons.uconn.edu/gs_theses/40

Cornell University

26. Posman, Kevin. Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3 .

Degree: 2013, Cornell University

URL: http://hdl.handle.net/1813/34294

▼ © 2013 Kevin M. Posman ! ABSTRACT Microbial processes shape ecosystem functions and the fate of environmental Contaminants. Despite their importance little is known about the active players carrying out specific metabolic processes in the environment. Investigations of environmental microbial communities are crucial for understanding the diversity of microorganisms capable of degrading these compounds and will provide insight into bioremediation strategies. The aim of this study was to use cultivation-dependent and cultivation-independent techniques to examine benzenedegrading microorganisms in hydrocarbon-contaminated groundwater. We investigated groundwater samples from a coal-tar contaminated aquifer in South Glens Falls, NY undergoing monitored natural attenuation (MNA). Previous stable isotope probing (SIP) experiments using 13 C-labelled benzene in groundwater microcosms from the site revealed a high abundance of !- and "-proteobacteria were active in the biodegradation of benzene. To further explore the microbiology of this system, we isolated benzene-degrading microorganisms from the SIP microcosm experiments. Nine organisms were isolated and identified using 16S rRNA gene sequences and several shared >97% nucleotide identity to 16S rRNA gene sequences retrieved in the SIP experiments. Isolate Variovorax MAK3 was chosen for further characterization. We designed primers to investigate a putative ring-hydroxylating dioxygenase (RHD) hypothesized to be involved in its ability to degrade benzene. We demonstrated that Variovorax MAK3 accelerated benzene degradation microcosms prepared from site waters and quantified a corresponding increase in Variovorax RHD gene expression over the same time period. Finally, we show that when the native community was exposed to benzene the ratio of RDH dioxygenase to 16S rRNA gene transcripts of native Variovorax populations increased over 6 fold during the benzene biodegradation. These data demonstrate how the convergence of cultivation-dependent ! and cultivation-independent techniques can lead to a precise understanding of active populations and their biodegradation genes in complex microbial communities. ! Advisors/Committee Members: Hewson, Ian (committeeMember).

Subjects/Keywords: microbiology; qPCR; benzene; bioremediation

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APA (6^th Edition):

Posman, K. (2013). Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3 . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/34294

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Posman, Kevin. “Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3 .” 2013. Thesis, Cornell University. Accessed February 20, 2020. http://hdl.handle.net/1813/34294.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Posman, Kevin. “Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3 .” 2013. Web. 20 Feb 2020.

Vancouver:

Posman K. Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3 . [Internet] [Thesis]. Cornell University; 2013. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/1813/34294.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Posman K. Benzene Biodegradation In Coal-Tar Contaminated Groundwater: Documenting The Role Of Variovorax Mak3 . [Thesis]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34294

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

27. Adams, Joshua Pope. POPLAR INTERACTIONS WITH ZINC FOR USE IN BIOREMEDIATION AND MONITORING.

Degree: PhD, Forestry, 2010, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10152010-084044/ ;

► Plant mechanisms regulating environmental heavy metal interactions are vital for plant survival. Plants must maintain adequate metal levels while preventing excesses. Several mechanisms involved… (more)

▼ Plant mechanisms regulating environmental heavy metal interactions are vital for plant survival. Plants must maintain adequate metal levels while preventing excesses. Several mechanisms involved in heavy metal uptake and sequestration have been identified and studied in hyperaccumulating plants such as Thlaspi caerulescens. These plants accumulate large quantities of metals, but their use in remediation is limited by their small size. On the other hand, mechanisms in high-biomass, non-hyperaccumulating perennial species such as poplar (Populus spp.) are unknown. The central goal of this project is to delineate specific mechanisms in poplar regulating the heavy metal zinc (Zn) for potential use in bioremediation and real-time monitoring. Specifically, project aims are: 1) Determine the role of HMA4 and PCS1 genes in poplar; 2) Delineate the ZIP gene family including ZIP1 and ZNT1 activity; and 3) Harness fluorescent energy transfer to engineer a poplar tree that monitors Zn-soil contamination. These are addressed using current technologies including phylogenetic analysis, gene transformation, expression assays, promoter-GUS assays, fluorescent-gene imaging, and metal assays. Through these experiments, mechanisms controlling heavy metal interaction are identified and characterized in poplar. Poplar contains a large number of genes in both the ZIP and HMA4 families, but only two members in the PCS family. Poplar also contains several genes that share close sequence and structural homology to those in hyperaccumulators. However, there is an overall divergence from hyperaccumulators in regards to expression across an environmental Zn gradient. Poplar tightly regulates Zn intake by suppressing absorption avenues under Zn excess. Over-expression of HMA4 and PCS1 resulted in more tolerance and more accumulation, respectively, in poplar lines. These findings support a regulatory system used in poplar to limit Zn under excess and promote Zn under deficiency. Using ZNT1 and its natural expression gradient, a chimeric protein was created that served as a biosensor in both poplar and Arabidopsis thaliana host plants and was able to discriminate between 1μM and 10mM Zn concentrations. These findings add to current knowledge of heavy metal regulation and help fill the gap of knowledge currently existing on the regulatory mechanisms that perennial trees use to control heavy metals. Advisors/Committee Members: M. Cetin Yuceer (chair), Emily B. Schultz (chair), Richard L. Harkess (committee member), Ardeshir Adeli (committee member), Grier P. Page (committee member), Claude W. dePamphilis (committee member).

Subjects/Keywords: poplar; zinc; bioremediation; biosensor

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APA (6^th Edition):

Adams, J. P. (2010). POPLAR INTERACTIONS WITH ZINC FOR USE IN BIOREMEDIATION AND MONITORING. (Doctoral Dissertation). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-10152010-084044/ ;

Chicago Manual of Style (16^th Edition):

Adams, Joshua Pope. “POPLAR INTERACTIONS WITH ZINC FOR USE IN BIOREMEDIATION AND MONITORING.” 2010. Doctoral Dissertation, Mississippi State University. Accessed February 20, 2020. http://sun.library.msstate.edu/ETD-db/theses/available/etd-10152010-084044/ ;.

MLA Handbook (7^th Edition):

Adams, Joshua Pope. “POPLAR INTERACTIONS WITH ZINC FOR USE IN BIOREMEDIATION AND MONITORING.” 2010. Web. 20 Feb 2020.

Vancouver:

Adams JP. POPLAR INTERACTIONS WITH ZINC FOR USE IN BIOREMEDIATION AND MONITORING. [Internet] [Doctoral dissertation]. Mississippi State University; 2010. [cited 2020 Feb 20]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10152010-084044/ ;.

Council of Science Editors:

Adams JP. POPLAR INTERACTIONS WITH ZINC FOR USE IN BIOREMEDIATION AND MONITORING. [Doctoral Dissertation]. Mississippi State University; 2010. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-10152010-084044/ ;

Colorado School of Mines

28. Dangelmayr, Martin A. Biogeochemical controls of uranium remediation and transport.

Degree: PhD, Civil and Environmental Engineering, 2018, Colorado School of Mines

URL: http://hdl.handle.net/11124/172151

► In the U.S. almost 140 sites have been contaminated by uranium mining and milling operations or by the storage of radioactive materials. In-situ recovery (ISR)… (more)

▼ In the U.S. almost 140 sites have been contaminated by uranium mining and milling operations or by the storage of radioactive materials. In-situ recovery (ISR) facilities still face challenges restoring water to pre-mining conditions and leave behind elevated uranium concentrations. Bioremediation and reactive transport modeling are potential tools to mitigate the impact of uranium contamination on human and environmental health, through their ability to immobilize uranium and assess the effectiveness of natural uranium attenuation. This project investigated biogeochemical aspects of both active and natural remediation of uranium contaminated subsurface for two field sites: The Smith Ranch Highland (SRH) site in WY, and the Rifle, Integrated Field Research Challenge (IFRC) site in CO. Our project objectives were to study the transformation of organic substrate during biostimulation and assess uranium retardation due to sorption with sediments taken from an ISR site. This thesis presents two research projects that address the remediation and risk assessment of uranium contaminated sites. The first project evaluated the impact of added organic carbon on the long-term biogeochemical attenuation of uranium in the subsurface of a former mill tailings site. Fluorescence and specific ultraviolet absorption (SUVA) analyses were used together with dissolved organic carbon (DOC) measurements to track organic carbon dynamics during and post-biostimulation of the 2011 Rifle IFRC experiment. An electron mass balance was performed on well CD01 to determine if any carbon sinks were unaccounted for. DOC values increased to 1.76 mM-C during biostimulation, and 3.18 mM-C post-biostimulation over background DOC values of 0.3-0.4 mM-C. Elevated DOC levels persisted 90 days after acetate injections ceased. The electron mass balance revealed that assumed electron acceptors would not account for the total amount of acetate consumed. Fluorescence spectra showed an increase in signals associated with soluble microbial products (SMP), during biostimulation, which disappeared post-biostimulation despite an increase in DOC. SUVA analyses, indicated that DOC present post-biostimulation is less aromatic in nature, compared to background DOC. Our results suggest that microbes convert injected acetate into a carbon sink that may be available to sustain iron reduction post-stimulation The second project consisted of two sets of column experiments and attempted to evaluate the impact of alkalinity and pH on the sorption of uranium in sediments from an ISR site. The ability of thermodynamic models to predict uranium behavior under conditions relevant to ISR restoration sites was also tested. Sediments at three different depths from a monitoring well at the SRH site were used in nine column studies and six batch experiments to study the sorption capacity of SRH sediments and estimate uncertainties associated with fitted parameters. Sediments were characterized by X-Ray Diffraction (XRD) and X-Ray Fluorescence (XRF) for dominant mineralogy and… Advisors/Committee Members: Figueroa, Linda A. (advisor), Stone, James J. (advisor), Sharp, Jonathan O. (committee member), Ranville, James F. (committee member), Bellona, Christopher (committee member).

Subjects/Keywords: Groundwater; Uranium; Sorption; Bioremediation

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APA (6^th Edition):

Dangelmayr, M. A. (2018). Biogeochemical controls of uranium remediation and transport. (Doctoral Dissertation). Colorado School of Mines. Retrieved from http://hdl.handle.net/11124/172151

Chicago Manual of Style (16^th Edition):

Dangelmayr, Martin A. “Biogeochemical controls of uranium remediation and transport.” 2018. Doctoral Dissertation, Colorado School of Mines. Accessed February 20, 2020. http://hdl.handle.net/11124/172151.

MLA Handbook (7^th Edition):

Dangelmayr, Martin A. “Biogeochemical controls of uranium remediation and transport.” 2018. Web. 20 Feb 2020.

Vancouver:

Dangelmayr MA. Biogeochemical controls of uranium remediation and transport. [Internet] [Doctoral dissertation]. Colorado School of Mines; 2018. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/11124/172151.

Council of Science Editors:

Dangelmayr MA. Biogeochemical controls of uranium remediation and transport. [Doctoral Dissertation]. Colorado School of Mines; 2018. Available from: http://hdl.handle.net/11124/172151

University of Waterloo

29. Greer, Karen D. Evaluation of an Oxygen Injection Technology for In-Situ Hydrocarbon Bioremediation in a Fractured Bedrock Environment.

Degree: 2009, University of Waterloo

URL: http://hdl.handle.net/10012/4357

► Oxygen has been shown to be an effective addition of enhancing the bioremediation of petroleum hydrocarbon contamination in porous media; however, the ability to effectively… (more)

▼ Oxygen has been shown to be an effective addition of enhancing the bioremediation of petroleum hydrocarbon contamination in porous media; however, the ability to effectively deliver oxygen to petroleum hydrocarbon contaminated groundwater has proven difficult. A field and numerical modelling study was completed at a former gas station in southern Ontario, to assess the delivery of oxygen into groundwater in a fractured limestone aquifer that had been contaminated with petroleum hydrocarbons. A field investigation was completed to characterize the bedrock aquifer and the groundwater flow system. Several hydraulically active fracture zones were identified and characterized. To evaluate how dissolved oxygen would behave in this type of groundwater environment, an injection test was completed using iTi’s gPro® oxygen injection technology. About 1000 L of water containing dissolved oxygen at ~ 30 mg/L and a bromide tracer was injected over ~ 90 minutes and monitored for ~ 10 days in the injection well and in a multilevel monitoring well located 3 metres down-gradient. The oxygen concentration rose rapidly within the injection well and at two of the down-gradient monitor intervals which were aligned with the injection well via major fractures. Concentration tailing persisted in the injection well for several days following injection. The effects of biodegradation were not assessed as part of this investigation. A three-dimensional numerical model for groundwater flow and advective-dispersive transport within a discretely-fractured porous medium was calibrated to the field conditions. The simulated injection test demonstrated that oxygen rapidly filled the porous matrix surrounding the injection well and filled the local intersecting fractures. Following injection, the oxygenated groundwater in the local fractures was rapidly flushed by the natural groundwater flow, with oxygen arrivals appearing as sharp pulses in the fracture-associated breakthrough curves in the monitor well. Back diffusion of oxygen from the porous matrix into the injection well was accurately reproduced by the model. Media properties (fracture apertures, hydraulic gradient and hydraulic conductivity) were varied to assess the sensitivity of the model and to evaluate the effectiveness of the remediation technology under different conditions. The sensitivity runs demonstrated that the distribution of oxygen within the system could be significantly different with varying degrees of advective transport within the fractures and diffusion into the rock matrix which depends on the physical properties and hydrogeological conditions. Predictive simulations were then run with two different injection scenarios: a continuous injection for 1 week and a cyclic injection scenario (injection every 2 days). The same mass of oxygen was delivered in each simulation (~3 kg). The results demonstrated that the delivery of oxygen into the system (continuous or cyclic) could affect the advective transport of oxygen through the fractures and the…

Subjects/Keywords: Bioremediation; Hydrocarbons

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APA (6^th Edition):

Greer, K. D. (2009). Evaluation of an Oxygen Injection Technology for In-Situ Hydrocarbon Bioremediation in a Fractured Bedrock Environment. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/4357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Greer, Karen D. “Evaluation of an Oxygen Injection Technology for In-Situ Hydrocarbon Bioremediation in a Fractured Bedrock Environment.” 2009. Thesis, University of Waterloo. Accessed February 20, 2020. http://hdl.handle.net/10012/4357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Greer, Karen D. “Evaluation of an Oxygen Injection Technology for In-Situ Hydrocarbon Bioremediation in a Fractured Bedrock Environment.” 2009. Web. 20 Feb 2020.

Vancouver:

Greer KD. Evaluation of an Oxygen Injection Technology for In-Situ Hydrocarbon Bioremediation in a Fractured Bedrock Environment. [Internet] [Thesis]. University of Waterloo; 2009. [cited 2020 Feb 20]. Available from: http://hdl.handle.net/10012/4357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Greer KD. Evaluation of an Oxygen Injection Technology for In-Situ Hydrocarbon Bioremediation in a Fractured Bedrock Environment. [Thesis]. University of Waterloo; 2009. Available from: http://hdl.handle.net/10012/4357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

30. Low, Adrian. Generation of a low pH 1,2-dichloroethane enrichment culture for enhanced bioremediation.

Degree: Biotechnology & Biomolecular Sciences, 2012, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52043 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10713/SOURCE01?view=true

► The successes of bioremediation field studies with organochlorine respiring bacteria have proved the efficacy of the method to degrade contaminants such as 1,2-dichloroethane (DCA) in… (more)

▼ The successes of bioremediation field studies with organochlorine respiring bacteria have proved the efficacy of the method to degrade contaminants such as 1,2-dichloroethane (DCA) in situ. The objective of this study was to demonstrate that a DCA degrading consortium, referred to here as AusDCA could be used to bioaugment a DCA contaminated acidic aquifer in situ. Functional characterisation experiments of AusDCA in batch cultures showed that the culture could dechlorinate high concentrations of DCA (6 mM) to ethene anaerobically at pH 5.5 and pH 6.5 and was not inhibited by approximately 15 µM of chloroform (CF). Phylogenetic analysis of an enrichment culture developed after several transfers into fresh medium and two dilution to extinction series of the parent AusDCA culture, identified two closely related Desulfitobacterium clades with sequences 93 and 95 % similarity to Desulfitobacterium metallireducens, a bacterium not known to dechlorinate DCA. Quantitative PCR (qPCR) of both clades demonstrated growth linked with DCA dihaloelimination to ethene at pH 5.5 and ethanol as the sole electron source. Characterisation of the putative DCA reductive dehalogenase, rdhA gene and the putative membrane anchoring protein, rdhB gene showed that the genes and the translated protein sequences were most similar to that of Dehalobacter strain WL, a DCA respiring bacteria from the Firmicutes phylum. This was surprising because not only is Dehalobacter a different genus to Desulfitobacterium, Dehalobacter was not characterised in community analysis of the parent AusDCA culture. A study to characterise the activity and mobility of filtered (planktonic) and unfiltered (planktonic and aggregated) AusDCA culture in aquifer matrix filled columns showed 1) the majority of the inocula had become entrained in the sandy aquifer and 2) aggregated cells are more active than planktonic cells. Application of the parent AusDCA culture in a bioaugmentation field study of the organochlorine contaminated acidic Botany Sands Aquifer, showed evidence of enhanced DCA degradation and longevity of up to 8 months in the bioaugmented well. This is based on in situ field and in vitro microcosm studies established using groundwater extracted 1 and 8 months after bioaugmentation and the detection of Desulfitobacterium specific to the AusDCA culture. This study has implications on bioaugmentation design relating to inoculation, feeding regime and pH buffering in sandy aquifers.

Subjects/Keywords: Organochlorine respiration; Bioremediation; 1,2-Dichloroethane

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Low, A. (2012). Generation of a low pH 1,2-dichloroethane enrichment culture for enhanced bioremediation. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52043 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10713/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Low, Adrian. “Generation of a low pH 1,2-dichloroethane enrichment culture for enhanced bioremediation.” 2012. Doctoral Dissertation, University of New South Wales. Accessed February 20, 2020. http://handle.unsw.edu.au/1959.4/52043 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10713/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Low, Adrian. “Generation of a low pH 1,2-dichloroethane enrichment culture for enhanced bioremediation.” 2012. Web. 20 Feb 2020.

Vancouver:

Low A. Generation of a low pH 1,2-dichloroethane enrichment culture for enhanced bioremediation. [Internet] [Doctoral dissertation]. University of New South Wales; 2012. [cited 2020 Feb 20]. Available from: http://handle.unsw.edu.au/1959.4/52043 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10713/SOURCE01?view=true.

Council of Science Editors:

Low A. Generation of a low pH 1,2-dichloroethane enrichment culture for enhanced bioremediation. [Doctoral Dissertation]. University of New South Wales; 2012. Available from: http://handle.unsw.edu.au/1959.4/52043 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:10713/SOURCE01?view=true

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