subject:(Biomedicine)

1. Nye, Alice C. Stages of Risk: Economies of Ambivalence in Cancer Genetics.

Degree: PhD, Theatre Arts and Performance Studies, 2014, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:386332/

► This dissertation examines the constitutive relation of gender and performance to knowledge production in the genetic sciences in twentieth and twenty-first century US. In recent… (more)

▼ This dissertation examines the constitutive relation of gender and performance to knowledge production in the genetic sciences in twentieth and twenty-first century US. In recent decades, a series of contradictions have emerged in the field of breast and ovarian cancer genetics that challenge common distinctions between biological nature and cultural artifice. From laboratory practices of cloning and patenting genes to medical practices of treating genetic risk as a disease by surgically removing healthy breasts and ovaries, this project argues that scientific understandings of genetic “natures” are drawing on mimetic “second natures” as a privileged mode of inquiry, evidence, and intervention. Combining archival and ethnographic research with critical analysis of medial, textual, and visual cultures, each chapter of this dissertation takes up a key paradox that inheres in the new genetics. In the process, it illuminates the rise of an alternative approach to knowledge production in which stakeholders are highlighting the ontological ambivalence of scientific knowledge objects, rather than treating them as natural or unmediated matters of fact. Across diverse social, scientific, clinical, economic, and legal domains, this project shows how practices of staging the artifice of biological nature and rendering mimesis apparent have come to characterize modes of knowing and intervening in bodies. This epistemic shift toward staging the constructedness of biological realities signals three broader mutations in contemporary economies of gender and technoscience. First, conventionally feminized modes of knowing such as affect, imitation, and relation are increasingly vital, not inimical, to scientific practice. Second, lay actors are playing a more active and critical role in producing and contesting genetic knowledge. And lastly, by staging, rather than effacing, the construction of genetic natures, lay and expert stakeholders are rendering newly apparent the uneven social, economic, and political landscape of women’s health. Advisors/Committee Members: Schneider, Rebecca (Director), Hamdy, Sherine (Reader), Ybarra, Patricia (Reader), Ridout, Nicholas (Reader).

Subjects/Keywords: Biomedicine

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APA (6^th Edition):

Nye, A. C. (2014). Stages of Risk: Economies of Ambivalence in Cancer Genetics. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:386332/

Chicago Manual of Style (16^th Edition):

Nye, Alice C. “Stages of Risk: Economies of Ambivalence in Cancer Genetics.” 2014. Doctoral Dissertation, Brown University. Accessed December 14, 2019. https://repository.library.brown.edu/studio/item/bdr:386332/.

MLA Handbook (7^th Edition):

Nye, Alice C. “Stages of Risk: Economies of Ambivalence in Cancer Genetics.” 2014. Web. 14 Dec 2019.

Vancouver:

Nye AC. Stages of Risk: Economies of Ambivalence in Cancer Genetics. [Internet] [Doctoral dissertation]. Brown University; 2014. [cited 2019 Dec 14]. Available from: https://repository.library.brown.edu/studio/item/bdr:386332/.

Council of Science Editors:

Nye AC. Stages of Risk: Economies of Ambivalence in Cancer Genetics. [Doctoral Dissertation]. Brown University; 2014. Available from: https://repository.library.brown.edu/studio/item/bdr:386332/

University of Helsinki

2. Lavilla-Alonso, Sergio. Adenovirus vectors with modified tropism for the treatment of colorectal cancer.

Degree: Haartman Institute, Department of Virology; Molecular Cancer Biology Program, Biomedicum, 2012, University of Helsinki

URL: http://hdl.handle.net/10138/32936

► Despite the rapid advance of cancer research and improvement of conventional therapeutic regimes like chemotherapy, surgery, immunotherapy or radiotherapy, cancer remains a leading cause of… (more)

▼ Despite the rapid advance of cancer research and improvement of conventional therapeutic regimes like chemotherapy, surgery, immunotherapy or radiotherapy, cancer remains a leading cause of death worldwide. Metastatic disease normally represents the most deadly stage and leads to the poorest prognosis. The disseminated location of metastases makes it even more difficult nowadays for existing drugs to target tumors without damaging healthy tissues. The work presented in this Doctoral Thesis is aimed at developing targeted adenoviral vectors and regimes that can be applied to the treatment of cancer, especially colorectal cancer. Gene therapists have explored widely interactions of the viruses to cancer and normal cells and have proved that molecular modifications in the capsid can unleash striking differences in viral tropism. In this Doctoral Thesis, the utility of arginine-glycine-aspartic acid (RGD) targeting αvβ integrins substituted for the lysine-lysine-threonine-lysine (KKTK) domain of the fiber shaft or inserted in the HI-loop of adenovirus serotype 5 (Ad5) was evaluated for increased tumor targeting and antitumor efficacy. Both modifications increased gene transfer efficacy in colorectal cancer cell lines and improved the tumor to-normal ratio after systemic administration of the vector. Furthermore, antitumor potency was not compromised with RGD modified viruses suggesting that an increased safety profile did not involve any loss of therapeutic effect. Treatments based on adenovirus vectors should not have negative effects on tumor progression or metastases. In order to evaluate this possibility, we designed a novel murine model of human colorectal cancer (CoCa) to test our treatments. To this end, we have developed a readily imageable mouse model of colorectal cancer featuring highly reproducible formation of spontaneous liver metastases derived from intrasplenic primary tumors. We optimized several experimental variables, and found that the correct choice of cell line and genetic background of the recipient mice as well as their age, were critical for the establishment of a useful animal model. A magnetic resonance imaging (MRI) protocol was optimized for use with this mouse model, and demonstrated to be a powerful method for analyzing the antitumor effects of an experimental therapy. Poor spreading of the virus through tumor tissue is one of the major issues limiting efficacy of oncolytic adenoviruses, even after local administration by intratumoral injection. In this study, ECM-degrading proteases relaxin, hyaluronidase, elastase, and macrophage metalloelastase (MME) were used to increase oncolytic adenovirus spreading. Moreover, MME improved the overall antitumor/antitumour efficacy of oncolytic adenovirus in subcutaneous HCT116 xenografts. In a liver metastatic colorectal cancer model, intratumoral treatment of HT29 primary tumors with MME monotherapy or with oncolytic adenovirus inhibited tumor growth. Combination therapy showed no increased mortality in comparison to monotherapies. In addition,…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lavilla-Alonso, S. (2012). Adenovirus vectors with modified tropism for the treatment of colorectal cancer. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/32936

Chicago Manual of Style (16^th Edition):

Lavilla-Alonso, Sergio. “Adenovirus vectors with modified tropism for the treatment of colorectal cancer.” 2012. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/32936.

MLA Handbook (7^th Edition):

Lavilla-Alonso, Sergio. “Adenovirus vectors with modified tropism for the treatment of colorectal cancer.” 2012. Web. 14 Dec 2019.

Vancouver:

Lavilla-Alonso S. Adenovirus vectors with modified tropism for the treatment of colorectal cancer. [Internet] [Doctoral dissertation]. University of Helsinki; 2012. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/32936.

Council of Science Editors:

Lavilla-Alonso S. Adenovirus vectors with modified tropism for the treatment of colorectal cancer. [Doctoral Dissertation]. University of Helsinki; 2012. Available from: http://hdl.handle.net/10138/32936

University of Helsinki

3. Zheng, Wei. Regulation of Angiogenesis and Lymphangiogenesis by Endothelial Cell Signaling Molecules.

Degree: Institute of Biomedicine, Research Program Unit, 2013, University of Helsinki

URL: http://hdl.handle.net/10138/42171

► Blood vessels deliver nutrients and oxygen to tissues, whereas lymphatic vessels collect interstitial fluid and absorb lipids. Both types of vessels are involved in immune… (more)

▼ Blood vessels deliver nutrients and oxygen to tissues, whereas lymphatic vessels collect interstitial fluid and absorb lipids. Both types of vessels are involved in immune surveillance. Malformation or malfunction of either vasculature is closely associated with various diseases. Although the molecular mechanisms regulating the growth, development and function of these vessels have begun to emerge in recent decades, many questions remain. This study aimed to understand if and how the growth of new blood and lymphatic vessels (angiogenesis and lymphangiogenesis, respectively) is regulated by the endothelial signaling molecules BMX, VEGFR-3, notch 1 and angiopoietin-2. BMX, a non-receptor tyrosine kinase, is upregulated in some types of cancer and promotes cell survival, migration and proliferation. Under physiological conditions, it is also highly expressed in blood endothelial cells (BECs). Thus, we hypothesized that BMX might contribute to tumor growth by promoting tumor angiogenesis. Using multiple tumor implantation and spontaneous tumor models in Bmx gene-deleted mice, we found that tumor angiogenesis and growth were significantly reduced in the absence of BMX. Conversely, when BMX was overexpressed in epithelial keratinocytes, chemical carcinogen led to increased tumor angiogenesis and growth. VEGFR-3 is a growth factor receptor on the surface of endothelial cells (ECs). Here, we characterized a blocking antibody that inhibits VEGFR-3 signaling by a novel mechanism. In contrast to conventional blocking antibodies that prevent ligand-receptor binding, this new antibody inhibited dimerization of two identical VEGFR-3 molecules, thereby impairing signaling activation and sprouting of lymphatic endothelial cells (LECs). These inhibitory effects persisted even at ligand concentrations so high that conventional blocking antibodies were no longer effective. Importantly, concurrent treatment with both types of antibodies yielded combined additive benefits. Notch signaling regulates angiogenic sprouting. We found in this study that Notch inhibitors enhance VEGF-induced lymphatic sprouting in 3-dimensional EC sprouting assays in vitro. In vivo, VEGF alone induced only lymphatic dilation but not sprouting. However, concomitant treatment with a Notch inhibitor induced both vascular events. In addition, a mosaic-sprouting assay showed that Notch signaling also determined the fates of tip/stalk cells (the cell leading a vessel sprout and the following cells, respectively) during lymphatic sprouting. Angiopoietin-2 (Ang2) is an endothelial growth factor required for proper lymphatic remodeling during development, but the exact mechanisms of this process have previously remained unclear. In contrast to the zipper-like pattern of cell-cell junctions (zippers) in collecting lymphatic vessels, mature lymphatic capillaries have a distinct pattern of button-like junctions (buttons), which transform from zippers during development. We found in this study that Ang2 is indispensable for such transformation of the junctional…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zheng, W. (2013). Regulation of Angiogenesis and Lymphangiogenesis by Endothelial Cell Signaling Molecules. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/42171

Chicago Manual of Style (16^th Edition):

Zheng, Wei. “Regulation of Angiogenesis and Lymphangiogenesis by Endothelial Cell Signaling Molecules.” 2013. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/42171.

MLA Handbook (7^th Edition):

Zheng, Wei. “Regulation of Angiogenesis and Lymphangiogenesis by Endothelial Cell Signaling Molecules.” 2013. Web. 14 Dec 2019.

Vancouver:

Zheng W. Regulation of Angiogenesis and Lymphangiogenesis by Endothelial Cell Signaling Molecules. [Internet] [Doctoral dissertation]. University of Helsinki; 2013. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/42171.

Council of Science Editors:

Zheng W. Regulation of Angiogenesis and Lymphangiogenesis by Endothelial Cell Signaling Molecules. [Doctoral Dissertation]. University of Helsinki; 2013. Available from: http://hdl.handle.net/10138/42171

University of Helsinki

4. Zarkada, Georgia. VEGFR3 and Notch signaling in angiogenesis.

Degree: Institute of Biomedicine, Faculty of Medicine; Wihuri Research Institute; Research Programs Unit, Translational Cancer Biology, 2014, University of Helsinki

URL: http://hdl.handle.net/10138/135563

► Blood and lymphatic vessels form extensive networks throughout the body, which function in order to deliver oxygen and nutrients to the tissues, to remove extravasated… (more)

▼ Blood and lymphatic vessels form extensive networks throughout the body, which function in order to deliver oxygen and nutrients to the tissues, to remove extravasated fluid and to absorb dietary lipids. The formation of new blood and lymphatic vessels (termed angiogenesis and lymphangiogenesis) is critical during embryonic development and in the adult, and is regulated by multiple signaling pathways. Vascular Endothelial Growth Factors (VEGFs) and their receptors (VEGFRs), as well as the Notch signaling system, are key governors of blood and lymphatic endothelial cell fate, and regulate angiogenesis and lymphangiogenesis in health and disease. Despite the numerous recent advances in the field of vascular biology, many steps in the complex processes of angiogenesis and lymphangiogenesis remain unclear. In this study we investigated the role of VEGFR3 signaling in blood endothelial cells, tip cell specification, as well as the interplay of the receptor with the VEGFR2 and the Notch signaling pathways during angiogenesis. VEGFR3 is a tyrosine kinase receptor that is mainly expressed in lymphatic endothelial cells in the adult. We observed VEGFR3 expression in sprouts that guide the blood vascular endothelium in angiogenic conditions. VEGFR3 blockade with a monoclonal antibody displayed synergistic properties with simultaneous VEGFR2 targeting in reducing angiogenesis and inhibiting tumor growth. Furthermore we found that Notch signaling suppresses VEGFR3 expression in endothelial cells, identifying VEGFR3 as a novel tip cell marker, which is normally repressed by Notch activation. In the next step we employed a combination of genetic and in vitro models to show that loss of VEGFR3 results in a hypervascular phenotype, accompanied by loss of Notch signaling. VEGFR3 could be stimulated by VEGFC and activate Notch in blood endothelial cells. Our results point towards a mechanism where VEGFC produced by macrophages at the vascular front acts via VEGFR3 to activate Notch and turn tip into stalk cells; thus promoting the formation of stable vascular loops. Furthermore we identified the transcription factor FOXC2 as the downstream target of the VEGFC/VEGFR3/Notch signaling cascade. These data reinforce the idea that VEGFR3 has two distinct signaling modalities, one ligand-dependent and one ligand-independent, and that different perturbations in VEGFR3 expression and function result in diverse vascular phenotypes. Subsequently we investigated the interplay of VEGFR2 and VEGFR3 in postnatal angiogenesis and lymphangiogenesis, using a genetic approach of conditional mutagenesis. Various combinations of genetic ablation of VEGFRs and pharmacological inhibition of Notch showed that VEGFR2 is irreplaceable during sprouting angiogenesis, also in endothelial cells with low Notch signaling, and that it acts upstream of VEGFR3 expression in angiogenic settings. On the other hand VEGFR3 suppressed VEGFR2 expression in a negative feedback loop. Finally we employed for the first time lymphatic endothelial specific deletion of VEGFRs…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zarkada, G. (2014). VEGFR3 and Notch signaling in angiogenesis. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/135563

Chicago Manual of Style (16^th Edition):

Zarkada, Georgia. “VEGFR3 and Notch signaling in angiogenesis.” 2014. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/135563.

MLA Handbook (7^th Edition):

Zarkada, Georgia. “VEGFR3 and Notch signaling in angiogenesis.” 2014. Web. 14 Dec 2019.

Vancouver:

Zarkada G. VEGFR3 and Notch signaling in angiogenesis. [Internet] [Doctoral dissertation]. University of Helsinki; 2014. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/135563.

Council of Science Editors:

Zarkada G. VEGFR3 and Notch signaling in angiogenesis. [Doctoral Dissertation]. University of Helsinki; 2014. Available from: http://hdl.handle.net/10138/135563

University of Helsinki

5. Wasik, Anita Agnieszka. Molecular mechanisms regulating glucose uptake into podocytes: Role of ezrin, septin 7 and nonmuscle myosin IIA.

Degree: Haartman Institute, 2014, University of Helsinki

URL: http://hdl.handle.net/10138/135955

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Diabetic nephropathy (DN) is a major microvascular complication of diabetes and a common cause of end-stage renal disease. Mechanisms leading to the development of DN… (more)

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Diabetic nephropathy (DN) is a major microvascular complication of diabetes and a common cause of end-stage renal disease. Mechanisms leading to the development of DN are not fully understood, but podocyte injury is involved. Interestingly, in respect to glucose uptake podocytes are uniquely insulin sensitive cells. Insulin rapidly induces remodeling of the actin cytoskeleton and leads to glucose uptake via glucose transporters GLUT1 and GLUT4. Defects in the trafficking of the glucose transporters may affect the insulin sensitivity of podocytes. Thus, regulators of glucose transporter trafficking may provide suitable targets to enhance insulin sensitivity of podocytes and prevent the development and progression of DN. However, the precise mechanisms regulating glucose transporter trafficking and glucose uptake into podocytes are largely uncharacterized. To identify changes in the expression of glomerular proteins at an early stage of DN we performed quantitative proteomic profiling of glomeruli isolated from rats with streptozotocin-induced diabetes and controls. Ezrin was found to be downregulated in diabetic glomeruli. In cultured podocytes depletion of ezrin increased glucose uptake by increasing translocation of GLUT1 to the plasma membrane. Loss of ezrin also induced actin remodelling, which involved cofilin-1. Phosphorylated cofilin-1 was upregulated in diabetic glomeruli suggesting altered actin dynamics. Furthermore, reduced expression of ezrin was found in the podocytes of human patients with diabetes. We found that the filament-forming septin 7 forms a complex with CD2AP and nephrin, both of which are essential for glomerular ultrafiltration. We showed that septin 7 negatively regulates GLUT4 storage vesicle (GSV) trafficking by forming a physical barrier between the vesicles and the plasma membrane. The novel interaction partner of septin 7, nonmuscle myosin heavy chain IIA (NMHC-IIA), was found to positively regulate insulin-stimulated glucose uptake into podocytes. Loss of NMHC-IIA reduced formation of the SNARE complex involved in GSV exocytosis. Furthermore, we presented that insulin regulates the association of septin 7 and phosphorylated RLC (pp-RLC), a part of myosin hexameric complex, with a plasma membrane SNARE, SNAP23. pp-RLC is upregulated in diabetic glomeruli and cultured human podocytes exposed to macroalbuminuric sera from patients with Type 1 diabetes. Our findings indicate that ezrin, septin 7 and NMHC-IIA regulate glucose uptake into podocytes and may play a role in the development of the renal complication in diabetes by regulating glucose transport and organization of the actin cytoskeleton in podocytes.

Diabeettinen munuaissairaus on diabetekseen liittyvä lisäsairaus. Diabeettisen munuaissairauden alkuvaiheen tunnusmerkki on valkuaisen vähäinen määrä virtsassa (mikroalbuminuria). Taudin edetessä valkuaisen määrä virtsassa lisääntyy ja voi johtaa varsinaiseen valkuaisvirtsaisuuteen. Diabeettisen munuaissairauden tarkkaa syntymekanismia ei tiedetä, mutta podosyyttien eli…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wasik, A. A. (2014). Molecular mechanisms regulating glucose uptake into podocytes: Role of ezrin, septin 7 and nonmuscle myosin IIA. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/135955

Chicago Manual of Style (16^th Edition):

Wasik, Anita Agnieszka. “Molecular mechanisms regulating glucose uptake into podocytes: Role of ezrin, septin 7 and nonmuscle myosin IIA.” 2014. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/135955.

MLA Handbook (7^th Edition):

Wasik, Anita Agnieszka. “Molecular mechanisms regulating glucose uptake into podocytes: Role of ezrin, septin 7 and nonmuscle myosin IIA.” 2014. Web. 14 Dec 2019.

Vancouver:

Wasik AA. Molecular mechanisms regulating glucose uptake into podocytes: Role of ezrin, septin 7 and nonmuscle myosin IIA. [Internet] [Doctoral dissertation]. University of Helsinki; 2014. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/135955.

Council of Science Editors:

Wasik AA. Molecular mechanisms regulating glucose uptake into podocytes: Role of ezrin, septin 7 and nonmuscle myosin IIA. [Doctoral Dissertation]. University of Helsinki; 2014. Available from: http://hdl.handle.net/10138/135955

University of Helsinki

6. Scifo, Enzo. Systematic Analysis of disease pathways in Congenital, Infantile and Juvenile Neuronal Ceroid Lipofuscinoses.

Degree: Institute of Biomedicine, Meilahti Clinical Proteomics Core Facility, 2014, University of Helsinki

URL: http://hdl.handle.net/10138/136286

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Neuronal ceroid lipofuscinoses (NCL) are common inherited childhood brain disorders. Since 1995, 13 known NCL causative genes (CLN1-8, CLN10-14) have been identified. Despite progress in… (more)

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Neuronal ceroid lipofuscinoses (NCL) are common inherited childhood brain disorders. Since 1995, 13 known NCL causative genes (CLN1-8, CLN10-14) have been identified. Despite progress in the NCL field, the primary function and physiological roles of most NCL proteins remain unresolved. In this thesis, we utilized various techniques, such as: functional proteomics, bioinformatics, and mouse disease models, in an effort to clarify disease pathways associated with congenital (CLN10), infantile, late-Infantile (CLN1, CLN5) and juvenile NCL (CLN3) in the human brain. Firstly, we examined the synaptic proteome in a cathepsin D (Ctsd / Cln10) knockout mouse model of congenital NCL, where the synaptic pathology resembles that of patients. Quantitative mass spectrometry analysis of mouse brain synaptosomes, showed that 43 proteins were differentially expressed in the Ctsd knockout mice brains. We used protein-protein interaction databases to generate a network of differentially expressed proteins and checked individual proteins for involvement in processes, pathways or disease phenotypes. This work highlighted defects in migratory functions of cathepsin D deficient cells that were attributed to downregulation of cytoskeletal proteins. We also aimed to map the CLN3-CLN5 protein interactome in the brain by identifying their associated proteins. Protein complexes from CLN3 or CLN5 expressing SH-SY5Y stable cells were analysed by mass spectrometry and processed by bioinformatics, to unravel molecular mechanisms underlying CLN3 and CLN5 diseases. Novel CLN3 and CLN5 interacting partners (42 and 31, respectively) were identified in this study. The extent of crosstalk amongst CLN3 and CLN5, suggests that the mechanisms leading to the functional deficits are shared between them. CLN3 was implicated in new roles of G-protein signalling and protein folding / sorting in the ER. Finally, we analysed protein complexes from human PPT1 (CLN1) expressing SH-SY5Y stable cells as described above. The goal of this study was to identify in vivo PPT1 substrates that could provide insight on the onset and progression of CLN1 disease. Our findings suggest putative new roles of PPT1 in neuronal migration and dopamine receptor mediated signalling pathway.

Neuronaaliset seroidilipofuskinoosit (NCL) ovat yleisimpiä lapsuusiässä ilmeneviä periytyviä aivosairauksia. Ensimmäinen NCL:ia aiheuttava geenivirhe julkaistiin jo vuonna 1995 ja sittemmin NCL:ia aiheuttavia alttiusgeenejä on löydetty jo yhteensä 13 (CLN1-8, CLN10-14). Tästä huolimatta useimpien NCL-tauteihin liittyvien geenien ja niiden koodaamien proteiinien ensisijaiset tehtävät soluissa ovat jääneet epäselviksi. Tässä väitöskirjatyössä on tutkittu NCL-tautien kongenitaaliseen (CLN10) sekä lapsuusiän (CLN1), myöhäisen lapsuusiän (CLN5) ja nuoruusiän ilmenemismuotoon johtavia muutoksia solujen toiminnassa hyödyntämällä funktionaalisen proteomiikan ja bioinformatiikan sovelluksia sekä kyseisiin tauteihin kehitettyjä hiirimalleja. Ensimmäisessä työssä hyödynsimme NCL:in kongenitaaliselle…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Scifo, E. (2014). Systematic Analysis of disease pathways in Congenital, Infantile and Juvenile Neuronal Ceroid Lipofuscinoses. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/136286

Chicago Manual of Style (16^th Edition):

Scifo, Enzo. “Systematic Analysis of disease pathways in Congenital, Infantile and Juvenile Neuronal Ceroid Lipofuscinoses.” 2014. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/136286.

MLA Handbook (7^th Edition):

Scifo, Enzo. “Systematic Analysis of disease pathways in Congenital, Infantile and Juvenile Neuronal Ceroid Lipofuscinoses.” 2014. Web. 14 Dec 2019.

Vancouver:

Scifo E. Systematic Analysis of disease pathways in Congenital, Infantile and Juvenile Neuronal Ceroid Lipofuscinoses. [Internet] [Doctoral dissertation]. University of Helsinki; 2014. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/136286.

Council of Science Editors:

Scifo E. Systematic Analysis of disease pathways in Congenital, Infantile and Juvenile Neuronal Ceroid Lipofuscinoses. [Doctoral Dissertation]. University of Helsinki; 2014. Available from: http://hdl.handle.net/10138/136286

University of Helsinki

7. Rantanen, Ville. Integration Platform for Biomedical Image Analysis.

Degree: Institute of Biomedicine, Research Programs Unit, 2015, University of Helsinki

URL: http://hdl.handle.net/10138/153857

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Images provide invaluable information to Biomedicine. Especially, microscopy as an information source has been providing knowledge for research and clinical diagnostics. We have moved away… (more)

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Images provide invaluable information to Biomedicine. Especially, microscopy as an information source has been providing knowledge for research and clinical diagnostics. We have moved away from simply looking at the images to quantifiable computerized image analysis. Over the last decades, image analysis developers have prepared algorithms and software to address various scientific enquiries using images. These software are often created for a single purpose. Naturally, not even the most generic software can include all the algorithms ever created. From an image analysis developer point of view, the choice of software creates limitations. It limits the developer to the algorithms included and to the language it was developed in. Even if the software is modular and extendable, a specific language is required and the earlier algorithm implementations would have to be ported. This thesis presents an integration platform for image analysis: Anima. It is capable of using existing software and including them in analysis workflows. Since image analysis is very case specific, custom processing commands are frequently needed. Anima comes with a large number of data and image analysis components developed directly for the platform, as well as components that send custom commands to the integrated software. All of the components can be executed in a single analysis pipeline. Anima itself is built on top of Anduril, another software, inheriting its software architecture. Anduril gives Anima the power of parallel processing and rerun prevention mechanism, speeding up the development cycle of new algorithms. The usability of Anima for method development is shown by implementing new segmentation algorithms and visualization tools. The tools and methods are all suited to large data sets. To display the modularity, the tools are published as separate programs that are then integrated in Anima. The usefulness of the platform is shown by applying it in different biomedical research settings. The settings include different organisms: human, rat and nematode; different sample material: brain tissue, lymphatic nodes and serum; and different medical interests: cerebral ischemia, cancer and allergy. Anima is a versatile open-source image analysis platform, that encourages the use of best practices of programming habits. It makes the development of analysis workflows and individual algorithms more efficient.

Kuvantaminen on tärkeä tiedon lähde lääketieteelle. Erityisesti mikroskopia on tärkeä kuvapohjaisen tiedon tuottaja biolääketieteellisessä tutkimuksessa. Tietokoneteknologian ansiosta emme ole enää riippuvaisia ihmissilmistä kuvien tulkitsijana. Viime vuosikymmeninä kuva-analyysien kehittäjät ovat luoneet algoritmeja ja kokonaisia ohjelmistoja kuvien hyödyntämiseksi tieteellisiin tarkoituksiin. Useimmat näistä ohjelmista tehdään yhtä tutkimuskysymystä varten. Edes yleisluontoiset ohjelmistopaketit eivät voi sisältää menetelmiä kaikkiin tarkoituksiin. Kuva-analyysikehittäjälle ohjelman valinta luo myös rajoituksia.…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rantanen, V. (2015). Integration Platform for Biomedical Image Analysis. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/153857

Chicago Manual of Style (16^th Edition):

Rantanen, Ville. “Integration Platform for Biomedical Image Analysis.” 2015. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/153857.

MLA Handbook (7^th Edition):

Rantanen, Ville. “Integration Platform for Biomedical Image Analysis.” 2015. Web. 14 Dec 2019.

Vancouver:

Rantanen V. Integration Platform for Biomedical Image Analysis. [Internet] [Doctoral dissertation]. University of Helsinki; 2015. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/153857.

Council of Science Editors:

Rantanen V. Integration Platform for Biomedical Image Analysis. [Doctoral Dissertation]. University of Helsinki; 2015. Available from: http://hdl.handle.net/10138/153857

University of Helsinki

8. Kuryk, Lukasz. STRATEGIES TO ENHANCE EFFICACY OF ONCOLYTIC VIROTHERAPY.

Degree: Centre for Drug Research (CDR) Division of Pharmaceutical Biosciences, 2016, University of Helsinki

URL: http://hdl.handle.net/10138/167783

► Despite major advances in conventional cancer treatments by surgery, chemotherapy, radiotherapy and their combination, the outcome remains partially ineffective against numerous cancer types, for example… (more)

▼ Despite major advances in conventional cancer treatments by surgery, chemotherapy, radiotherapy and their combination, the outcome remains partially ineffective against numerous cancer types, for example mesothelioma, lung cancer, and colon cancer. Furthermore, due to resistance factors and the subsequent loss of response, which may occur rapidly during the conventional treatments regimes, new anti-cancer agents, presenting new mechanisms of action and lacking cross-resistance to commonly used therapies, are in high demand. Oncolytic virotherapy is a promising anti-cancer strategy, and the approval of the first oncolytic virus, Imlygic (T-Vec, talimogene laherparepvec), in Western world by US Food and Drug Administration (FDA) and European Medicines Agency (EMA) has opened up new perspectives for improved treatment of cancer. Single therapy is rarely successful in treating cancer, particularly in metastatic or advanced cancer, and survival rates with monotherapies alone are generally poor. The combination of multiple therapies to treat cancer has already shown significant results in the standard care of cancer. This strategy utilizes the combination of both conventional and novel therapies that can bring the future promise of cancer treatment. In this thesis it has been hypothesized that by combining oncolytic adenoviruses (oAd) with chemotherapeutic drugs and a biological agent we could improve anti-cancer efficacy through synergistic effect against cancer. Therefore, we have tested various treatment regimes with the overall goal being the improvement of oncolytic virotherapy efficacy. Secondly, since safety issues concerning gene therapy and viral vectors are tremendously important, we have performed studies on safety issues of adenoviral vectors. In brief, we have evaluated the anti-cancer activity of combination treatment with standard of care (SoC) chemotherapy (Pemetrexed, Cisplatin, Carboplatin) and Ad5/3-d24-GM-CSF (ONCOS-102) in vitro and in a xenograft BALB/c model of human malignant mesothelioma (MM). We could show improved anti-tumor effects when ONCOS-102 was combined with SoC chemotherapy regimens over chemotherapy and virus alone. Combination therapy resulted in synergistic anti-cancer effect improving the therapeutic outcome. In a subsequent study we tested anti-cancer properties of the dipeptide L-Carnosine complexed with an oncolytic adenovirus (virus-L-Carnosine complex). The complex demonstrated improved anti-tumor efficacy both in vitro and in vivo in tested cancer models. In HCT116 colon and A549 lung cancer cells, the virus-L-Carnosine complex presented a higher transduction level and infectious titer over uncoated oncolytic adenovirus. The in vivo efficacy of the virus-L-Carnosine complex was tested in two cancer models: i) lung and ii) colon cancer xenograft mice models. It exhibited a significant reduction in tumor growth compared to other tested groups. Additionally, we investigated the molecular mechanism underlying the effects of the complex on tumor growth reduction. Safety…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuryk, L. (2016). STRATEGIES TO ENHANCE EFFICACY OF ONCOLYTIC VIROTHERAPY. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/167783

Chicago Manual of Style (16^th Edition):

Kuryk, Lukasz. “STRATEGIES TO ENHANCE EFFICACY OF ONCOLYTIC VIROTHERAPY.” 2016. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/167783.

MLA Handbook (7^th Edition):

Kuryk, Lukasz. “STRATEGIES TO ENHANCE EFFICACY OF ONCOLYTIC VIROTHERAPY.” 2016. Web. 14 Dec 2019.

Vancouver:

Kuryk L. STRATEGIES TO ENHANCE EFFICACY OF ONCOLYTIC VIROTHERAPY. [Internet] [Doctoral dissertation]. University of Helsinki; 2016. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/167783.

Council of Science Editors:

Kuryk L. STRATEGIES TO ENHANCE EFFICACY OF ONCOLYTIC VIROTHERAPY. [Doctoral Dissertation]. University of Helsinki; 2016. Available from: http://hdl.handle.net/10138/167783

9. Chen, Ping. Quantitative study of transcriptome dynamics during evolution and treatment resistance in cancer.

Degree: Institute of Biomedicine, Research Programs Unit, Genome-Scale Biology and Medicum, 2016, University of Helsinki

URL: http://hdl.handle.net/10138/159335

►

Transcriptome, defined by the collection of all RNA molecules in a cell, acts as a central bridge that transfers genetic information into molecular functions. Transcriptome… (more)

▼

Transcriptome, defined by the collection of all RNA molecules in a cell, acts as a central bridge that transfers genetic information into molecular functions. Transcriptome regulates the biological characteristics in all living organisms, thus it is one of the most important research subjects in biology. RNAs are transcribed at different levels tightly controlled by cellular conditions. This produces great diversity in cellular transcriptome dynamics, introducing a lot of complexity to the transcriptomic research. Though tremendous challenges exist, the study of transcriptome dynamics is essential to the understanding of the complex systems within the cells and cellular behavior. The dynamics of transcriptome can be investigated by high-throughput technologies, such as microarrays and RNA-sequencing. The large amounts of data introduces challenges to data management, analysis and interpretation. To generate biologically testable and conclusive results, efficient computational methods are urgently needed. This thesis includes theoretical and methodological research. The theoretical part of the research comprehensively studies the characteristics of gene expression, the splicing of ancient and novel exons during the evolution by comparative analysis on transcriptomic data of nine tissues from five species. The methodological research includes new methods developed to solve the research questions related to the study of transcriptomic dynamics in evolution and cancer. The main methods developed in this thesis are 1) exon age classifier, which is able to classify exons according to their evolutionary time, providing the basis for the theoretical study in this thesis; 2) MEAP, a new exon array preprocessing method for expression quantification at multi-levels; 3) PSFinder, a new approach to identify patient prognostic subgroups from treatment naive tumor samples based on their transcriptomic profiles and associated clinical survival times. The theoretical part gives a comprehensive view on the mechanisms of dynamic changes during the evolution of the transcriptome, which provides a solid theoretical basis to the methodological part. The application of MEAP and PSFinder to high-grade serous ovarian cancer revealed a small set of isoform markers with distinct expression profiles for patient prognosis stratification. In combination with experimental validation, the results demonstrate the applicability of these methods in the quantification and stratification of tumor transcriptome dynamics, which provides new insights to the clinical diagnosis and precision medicine for human cancers.

转录组是细胞内所有RNA分子的集合,它将细胞内遗传信息编译为具体的功能性分子。转录组调节了生物体内的各项生物学特性,是生物学中的重要研究对象之一。在细胞各分子机制的严密调节控制下,RNA被转录为不同的水平。这产生了细胞转录动态中的多样性,从而给转录组学的研究带来许多复杂性。因此转录组动态的研究对了解细胞中的复杂系统及细胞行为极为重要,但也意味着转录组研究的难度较高。转录组的动态可以通过高通量技术,例如表达芯片及RNA测序来研究。大量的数据给数据管理,分析及解释带来了挑战。有效的计算分析方法,是生成生物学上可检测和确定性结果的关键。本文包含了理论和方法的研究。该研究的理论部分对来自五种物种九种组织的转录组数据进行比较分析,综合研究了在进化过程中基因表达及新老外显子的剪切特性。该论文的方法学研究包括了为了解决进化及癌症中转录组动态研究的相关问题而开发的新算法。本文中开发的主要方法有: 1) 外显子分类器,它能够根据…

Subjects/Keywords: biomedicine; biomedicine

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, P. (2016). Quantitative study of transcriptome dynamics during evolution and treatment resistance in cancer. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/159335

Chicago Manual of Style (16^th Edition):

Chen, Ping. “Quantitative study of transcriptome dynamics during evolution and treatment resistance in cancer.” 2016. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/159335.

MLA Handbook (7^th Edition):

Chen, Ping. “Quantitative study of transcriptome dynamics during evolution and treatment resistance in cancer.” 2016. Web. 14 Dec 2019.

Vancouver:

Chen P. Quantitative study of transcriptome dynamics during evolution and treatment resistance in cancer. [Internet] [Doctoral dissertation]. University of Helsinki; 2016. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/159335.

Council of Science Editors:

Chen P. Quantitative study of transcriptome dynamics during evolution and treatment resistance in cancer. [Doctoral Dissertation]. University of Helsinki; 2016. Available from: http://hdl.handle.net/10138/159335

University of Helsinki

10. Jokinen, Riikka. Genetic Studies of Tissue-Specific Mitochondrial DNA Segregation in Mammals.

Degree: Institute of Biomedicine, Research Programs Unit, Molecular Neurology, 2016, University of Helsinki

URL: http://hdl.handle.net/10138/160103

►

Mitochondrial DNA (mtDNA) is a small extra-nuclear genome present in all nucleated cells of the body and important for mitochondrial function. The mtDNA is a… (more)

▼

Mitochondrial DNA (mtDNA) is a small extra-nuclear genome present in all nucleated cells of the body and important for mitochondrial function. The mtDNA is a present in hundreds to thousands of copies per cell and therefore arising mutations cause heteroplasmy: the co-existence of two or more distinct mtDNA variants in the same cell. Because of these features mtDNA variants segregate mitotically in the tissues of an individual, which can lead to time-dependent changes in the relative proportions of the mtDNA variants. Mutations in the mtDNA cause diseases and most pathogenic mtDNA mutations are heteroplasmic. In heteroplasmic situations a certain threshold proportion of the mutant mtDNA must be exceeded prior to onset of symptoms. Somatic mtDNA segregation of mtDNA mutations affect whether the threshold is exceeded, and can thus be a factor in determining disease onset and severity. Some pathogenic mtDNA mutations exhibit tissue-specific mtDNA segregation patterns, but the genes and mechanisms involved in this process are unknown. The aim of this thesis was to uncover genetic regulators of tissue-specific mtDNA segregation and study their properties to gain insight into the mechanisms involved in this process. We investigated tissue-specific mtDNA segregation in a mouse model that segregates two neutral mtDNA variants. These mtDNA variants display tissue-specific mtDNA segregation in three tissue types: the liver, kidney and hematopoietic tissues. In these tissues there is selection for one mtDNA variant over the other. Using this mouse model we identified and verified Gimap3 as a modifying gene for mtDNA segregation in the hematopoietic tissues. In a follow-up study we further studied Gimap3 and a functionally related gene Gimap5. We uncovered a novel subcellular localization to the endoplasmic reticulum for the Gimap3 protein. Moreover we established Gimap5, which encodes a lysosomal protein, as another genetic modifier of mtDNA segregation in hematopoietic tissues. Taken together these results demonstrated the involvement of other organelles in the segregation of mtDNA. To study tissue-specific mtDNA segregation from another aspect we investigated the role of mitochondrial fission in this process. Mitochondrial fission has been implicated to play a role in mtDNA segregation in yeast. We utilized a dominant-negative mouse model for Dnm1l, a master regulator of mitochondrial fission. We demonstrated that expression of the dominant-negative Dnm1l modulated the mtDNA segregation specifically in the hematopoietic tissues. In conclusion, we were able discover the first genetic modifiers for tissue-specific mtDNA segregation in mammals. These findings can be utilized to guide future research aiming to uncover the molecular mechanisms of this process, which can ultimately elucidate the genetics of pathogenic human mtDNA mutations.

Mitokondriot ovat tärkeitä soluelimiä, joita on kaikissa ihmisen soluissa lukuun ottamatta veren punasoluja. Yksi tärkeimmistä mitokondrion monista tehtävistä on aerobinen…

Subjects/Keywords: biomedicine; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jokinen, R. (2016). Genetic Studies of Tissue-Specific Mitochondrial DNA Segregation in Mammals. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/160103

Chicago Manual of Style (16^th Edition):

Jokinen, Riikka. “Genetic Studies of Tissue-Specific Mitochondrial DNA Segregation in Mammals.” 2016. Doctoral Dissertation, University of Helsinki. Accessed December 14, 2019. http://hdl.handle.net/10138/160103.

MLA Handbook (7^th Edition):

Jokinen, Riikka. “Genetic Studies of Tissue-Specific Mitochondrial DNA Segregation in Mammals.” 2016. Web. 14 Dec 2019.

Vancouver:

Jokinen R. Genetic Studies of Tissue-Specific Mitochondrial DNA Segregation in Mammals. [Internet] [Doctoral dissertation]. University of Helsinki; 2016. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10138/160103.

Council of Science Editors:

Jokinen R. Genetic Studies of Tissue-Specific Mitochondrial DNA Segregation in Mammals. [Doctoral Dissertation]. University of Helsinki; 2016. Available from: http://hdl.handle.net/10138/160103

University of Hawaii – Manoa

11. Hemrajani, Aashish. The Dharma of Bums: Identity, Neoliberalism, and Biomedicine in the Practice of Treating Homelessness in Honolulu.

Degree: 2017, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/51533

►

M.A. University of Hawaii at Manoa 2016.

Focusing on Honolulu, the city with the highest rate of homelessness in the United States, this thesis demonstrates… (more)

Subjects/Keywords: Homelessness; Biomedicine; Identity

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APA (6^th Edition):

Hemrajani, A. (2017). The Dharma of Bums: Identity, Neoliberalism, and Biomedicine in the Practice of Treating Homelessness in Honolulu. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/51533

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hemrajani, Aashish. “The Dharma of Bums: Identity, Neoliberalism, and Biomedicine in the Practice of Treating Homelessness in Honolulu.” 2017. Thesis, University of Hawaii – Manoa. Accessed December 14, 2019. http://hdl.handle.net/10125/51533.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hemrajani, Aashish. “The Dharma of Bums: Identity, Neoliberalism, and Biomedicine in the Practice of Treating Homelessness in Honolulu.” 2017. Web. 14 Dec 2019.

Vancouver:

Hemrajani A. The Dharma of Bums: Identity, Neoliberalism, and Biomedicine in the Practice of Treating Homelessness in Honolulu. [Internet] [Thesis]. University of Hawaii – Manoa; 2017. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10125/51533.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hemrajani A. The Dharma of Bums: Identity, Neoliberalism, and Biomedicine in the Practice of Treating Homelessness in Honolulu. [Thesis]. University of Hawaii – Manoa; 2017. Available from: http://hdl.handle.net/10125/51533

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

12. Stoltsz, Werner Heinrich. Comparison of resting state functional networks in HIV infected and uninfected children at age 9 years.

Degree: Med, Division of Biomedical Engineering, 2018, University of Cape Town

URL: http://hdl.handle.net/11427/29582

► Over 2.5 million children are infected with HIV, the majority of whom reside in Sub-Saharan Africa. Treatment coverage is steadily gaining momentum, reducing mortality and… (more)

▼ Over 2.5 million children are infected with HIV, the majority of whom reside in Sub-Saharan Africa. Treatment coverage is steadily gaining momentum, reducing mortality and morbidity. Yet little is known about brain development in HIV-infected (HIV+) children who are on highly-active antiretroviral therapy (ART), with viral load suppression from a young age. Here, we use resting state fMRI (rs-fMRI) to examine the impact of HIV and ART on the development of functional networks in 9-year-old vertically HIV-infected children compared to age-matched controls of similar socioeconomic status. We present analyses for a sample of 40 HIV+ (9.2 ± 0.20 years; 16 males) children from the Children with HIV Early Antiretroviral (CHER) clinical trial (Cotton et al. 2013; Violari et al. 2008) and 24 uninfected (12 exposed; 12 males; 9.6 ± 0.52 years) controls from an interlinking vaccine trial (Madhi et al. 2010). Scans were performed at the Cape Universities Body Imaging Centre (CUBIC) in Cape Town, South Africa. We investigated HIV-related differences in within- and between-network functional connectivity (FC) using independent component analysis(ICA) and seed-based correlation analysis (SCA). For SCA, seeds were placed in the structural core, in regions implicated in HIV-related between-group differences at age 7 years, and in regions associated with neuropsychological domains impaired in our cohort. In addition, we evaluated associations of past and present immune health measures with within-network connectivity using ICA. We found no HIV-related intra-network FC differences within any ICA-generated RSNs at age 9 years, perhaps as a result of within-network connectivity not being sufficiently robust at this age. We found a positive association of CD4%, both current and in infancy, with functional integration of left lobule 7 into the cerebellum network at age 9 years. Long-term impact of early immune health supports recently-revised policies of commencing ART immediately in HIV+ neonates. ii Compared to uninfected children, HIV+ children had increased FC to several seeds. Firstly, to seeds associated with the planning and visual perception neuropsychological domains. Secondly, to structural core seeds in the extrastriate visual cortex (of the medial visual network) and the right angular gyrus (of the temporoparietal network). Finally, to left paracentral (somatosensory network) and right precuneus (posterior DMN) seeds previously revealing between-group differences at age 7 years. The connections with greater FC in HIV+ children may variously indicate functional recruitment of additional brain capacity, immature excess of short-range connections, and/or immature excess of between-network connections. In conclusion, despite early ART and early virologic suppression, HIV+ children demonstrate instances of abnormal FC at age 9 years. Disruption to visual cortex is marked, consistent with indications from neuropsychological testing that visual perception is disrupted. The profile of HIV- and/or ART-related effects on FC differs… Advisors/Committee Members: Meintjes, Ernesta M (advisor), du Plessis, Lindie (advisor).

Subjects/Keywords: Biomedicine; Biomedical Engineering

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APA (6^th Edition):

Stoltsz, W. H. (2018). Comparison of resting state functional networks in HIV infected and uninfected children at age 9 years. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/29582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stoltsz, Werner Heinrich. “Comparison of resting state functional networks in HIV infected and uninfected children at age 9 years.” 2018. Thesis, University of Cape Town. Accessed December 14, 2019. http://hdl.handle.net/11427/29582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stoltsz, Werner Heinrich. “Comparison of resting state functional networks in HIV infected and uninfected children at age 9 years.” 2018. Web. 14 Dec 2019.

Vancouver:

Stoltsz WH. Comparison of resting state functional networks in HIV infected and uninfected children at age 9 years. [Internet] [Thesis]. University of Cape Town; 2018. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/11427/29582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stoltsz WH. Comparison of resting state functional networks in HIV infected and uninfected children at age 9 years. [Thesis]. University of Cape Town; 2018. Available from: http://hdl.handle.net/11427/29582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

13. Thompson, Seth Mkhanyisi. Optimisation of insertion point during latissimus dorsi tendon transfer.

Degree: Med, Division of Biomedical Engineering, 2018, University of Cape Town

URL: http://hdl.handle.net/11427/29524

► Problem and Motivation: Posterior rotator cuff injuries are common (Yamaguchi et al., 2006), (Neri et al., 2009) and often debilitating and irreparable (Sim et al.,… (more)

▼ Problem and Motivation: Posterior rotator cuff injuries are common (Yamaguchi et al., 2006), (Neri et al., 2009) and often debilitating and irreparable (Sim et al., 2001). Latissimus dorsi (LD) tendon transfers have been shown to be an effective treatment for these massive or irreparable tears (Habermeyer, 2006), (De Casas et al., 2014). This procedure can have unpredictable outcomes (Ling et al., 2009). This is partially caused by discrepancies in the suggested insertion site for the LD tendon during transfers. The current literature is composed of in-silico studies which ignore the practicalities of the human body (Magermans et al., 2004), in-vivo studies which use subjective pain scores, and small scale cadaver trials. For these reasons, a study is needed that uses the power of in-silico modeling in a way that is verified using in-vitro testing on cadavers. Aims and Objectives: The aim of this study is to determine the effects of varying the insertion point of the LD tendon on the humeral head to treat posterior rotator cuff tears in terms of the effects on strength, primarily in rotation and in flexion over a range of motion. The objectives are to use an in-silico model to define the effects of various insertion points and validate this model using a cadaver trial before presenting the final findings. Methods: In-silico Model The Upper Extremity Model (Holzbaur et al., 2005) was used to simulate tendon transfers. The moment arms in flexion and rotation were measured and recorded at angles of 0° and 90° of forward ix elevation. The moment arms at each point were then projected onto humeral maps to display the results. Cadaver Trial Four fresh frozen cadaver torsos (eight shoulders) were mounted into a specifically designed rig. The LD was transferred to 7 points illustrative of the humeral head. The strain generated by the humerus in rotation on the clamps was measured at 0° and 90° of forward flexion for each point. These were then compared. Results In-silico Model The in-silico moment arm maps were generated and analysed. The optimal point for external rotation at 0° of flexion was the lesser tuberosity. Moment arms to produce external rotation were found over the entire greater tuberosity. Flexion was only generated on the posterior edge of the greater tuberosity. At 90° of flexion, little to no rotation generating moment arms were found in the lesser tuberosity and the anterior ridge of the greater tuberosity. Rotation generating moment arms were not significantly different between the posterior edge and the face of the greater tuberosity. No areas generated flexion moment arms. Cadaver Trial At 0° of flexion, the lesser tuberosity (point 1) generated the most flexion, with the greater tuberosity (points 2-7) also generating external rotation, but at reduced levels. At 90° of flexion, the lesser tuberosity and the anterior ridges of the greater tuberosty (points 1-3) generated no significant rotation. The posterior ridge and face of the greater tuberosity generated similar amounts of flexion, greater… Advisors/Committee Members: Sivarasu, Sudesh (advisor), Roche, Stephen (advisor).

Subjects/Keywords: Biomedicine; Biomedical Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thompson, S. M. (2018). Optimisation of insertion point during latissimus dorsi tendon transfer. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/29524

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thompson, Seth Mkhanyisi. “Optimisation of insertion point during latissimus dorsi tendon transfer.” 2018. Thesis, University of Cape Town. Accessed December 14, 2019. http://hdl.handle.net/11427/29524.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thompson, Seth Mkhanyisi. “Optimisation of insertion point during latissimus dorsi tendon transfer.” 2018. Web. 14 Dec 2019.

Vancouver:

Thompson SM. Optimisation of insertion point during latissimus dorsi tendon transfer. [Internet] [Thesis]. University of Cape Town; 2018. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/11427/29524.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thompson SM. Optimisation of insertion point during latissimus dorsi tendon transfer. [Thesis]. University of Cape Town; 2018. Available from: http://hdl.handle.net/11427/29524

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

14. Moore, Cody. Beyond Biomedicine: Developing New Models of Medical Practice from the Pragmatist and Existentialist Traditions.

Degree: 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11099

► This thesis seeks to address two distinct sets of criticisms that have been offered at medical practice. The first criticism suggests that medicine today is… (more)

▼ This thesis seeks to address two distinct sets of criticisms that have been offered at medical practice. The first criticism suggests that medicine today is too exclusive in its application of the term 'disease.' As a consequence, important biological phenomena are marginalized by physicians and scientists. The second criticism suggests that medicine has been too inclusive in its understanding of disease. As a result, many biological phenomena that were once considered 'natural' or 'normal' aspects of human life are now given a medical dimension that they previously did not have. The goal of this thesis is to understand why two seemingly contradictory criticisms have been applied to the same practice. To answer this question, I invoke Edmund Husserl's important analysis of modern science to argue that medicine suffers from a problem of 'naive objectivism.' This problem is present under the dominant paradigm of medical diagnosis, the biomedical model. Having identified the source of these two criticisms, my goal is to then develop new models of medical practice that can address these criticisms. First, I turn to John Dewey's philosophical naturalism to develop a medical model that can address the problem of exclusion in biomedicine. Then, I turn to Martin Heidegger?s existential analytic to develop a medical model that can address the problem of inclusion in biomedicine. I supplement both of these analyses with research generated in the medical humanities fields, attempting to show how the biomedical model of medicine fails to meet the goals of medical care. The end result of such analysis is the development of two new medical models that can serve to replace the biomedical model. I offer no attempt to adjudicate between these two models, instead leaving such issues to be handled by the patient and the physician throughout the course of his or her treatment. Advisors/Committee Members: McDermott, John J. (advisor), George, Theodore D. (committee member), Sanders, Charles W. (committee member).

Subjects/Keywords: Medical Humanities; Existentialism; Pragmatism; Biomedicine

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APA (6^th Edition):

Moore, C. (2012). Beyond Biomedicine: Developing New Models of Medical Practice from the Pragmatist and Existentialist Traditions. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11099

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moore, Cody. “Beyond Biomedicine: Developing New Models of Medical Practice from the Pragmatist and Existentialist Traditions.” 2012. Thesis, Texas A&M University. Accessed December 14, 2019. http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11099.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moore, Cody. “Beyond Biomedicine: Developing New Models of Medical Practice from the Pragmatist and Existentialist Traditions.” 2012. Web. 14 Dec 2019.

Vancouver:

Moore C. Beyond Biomedicine: Developing New Models of Medical Practice from the Pragmatist and Existentialist Traditions. [Internet] [Thesis]. Texas A&M University; 2012. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11099.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moore C. Beyond Biomedicine: Developing New Models of Medical Practice from the Pragmatist and Existentialist Traditions. [Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11099

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

15. Florez Vargas, Oscar Roberto. DEVELOPMENT OF STRATEGIES FOR ASSESSING REPORTING IN BIOMEDICAL RESEARCH: MOVING TOWARD ENHANCING REPRODUCIBILITY.

Degree: 2016, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:302324

► The idea that the same experimental findings can be reproduced by a variety of independent approaches is one of the cornerstones of science’s claim to… (more)

▼ The idea that the same experimental findings can be reproduced by a variety of independent approaches is one of the cornerstones of science’s claim to objective truth. However, in recent years, it has become clear that science is plagued by findings that cannot be reproduced and, consequently, invalidating research studies and undermining public trust in the research enterprise. The observed lack of reproducibility may be a result, among other things, of the lack of transparency or completeness in reporting. In particular, omissions in reporting the technical nature of the experimental method make it difficult to verify the findings of experimental research in biomedicine. In this context, the assessment of scientific reports could help to overcome – at least in part – the ongoing reproducibility crisis.In addressing this issue, this Thesis undertakes the challenge of developing strategies for the evaluation of reporting biomedical experimental methods in scientific manuscripts. Considering the complexity of experimental design – often involving different technologies and models, we characterise the problem in methods reporting through domain-specific checklists. Then, by using checklists as a decision making tool, supported by miniRECH – a spreadsheet-based approach that can be used by authors, editors and peer-reviewers – a reasonable level of consensus on reporting assessments was achieved regardless of the domain-specific expertise of referees. In addition, by using a text-mining system as a screening tool, a framework to guide an automated assessment of the reporting of bio-experiments was created. The usefulness of these strategies was demonstrated in some domain-specific scientific areas as well as in mouse models across biomedical research.In conclusion, we suggested that the strategies developed in this work could be implemented through the publication process as barriers to prevent incomplete reporting from entering the scientific literature, as well as promoters of completeness in reporting to improve the general value of the scientific evidence. Advisors/Committee Members: STEVENS, ROBERT RD, Brass, Andrew, Stevens, Robert.

Subjects/Keywords: Reproducibility; Checklists; Text Mining; Biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Florez Vargas, O. R. (2016). DEVELOPMENT OF STRATEGIES FOR ASSESSING REPORTING IN BIOMEDICAL RESEARCH: MOVING TOWARD ENHANCING REPRODUCIBILITY. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:302324

Chicago Manual of Style (16^th Edition):

Florez Vargas, Oscar Roberto. “DEVELOPMENT OF STRATEGIES FOR ASSESSING REPORTING IN BIOMEDICAL RESEARCH: MOVING TOWARD ENHANCING REPRODUCIBILITY.” 2016. Doctoral Dissertation, University of Manchester. Accessed December 14, 2019. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:302324.

MLA Handbook (7^th Edition):

Florez Vargas, Oscar Roberto. “DEVELOPMENT OF STRATEGIES FOR ASSESSING REPORTING IN BIOMEDICAL RESEARCH: MOVING TOWARD ENHANCING REPRODUCIBILITY.” 2016. Web. 14 Dec 2019.

Vancouver:

Florez Vargas OR. DEVELOPMENT OF STRATEGIES FOR ASSESSING REPORTING IN BIOMEDICAL RESEARCH: MOVING TOWARD ENHANCING REPRODUCIBILITY. [Internet] [Doctoral dissertation]. University of Manchester; 2016. [cited 2019 Dec 14]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:302324.

Council of Science Editors:

Florez Vargas OR. DEVELOPMENT OF STRATEGIES FOR ASSESSING REPORTING IN BIOMEDICAL RESEARCH: MOVING TOWARD ENHANCING REPRODUCIBILITY. [Doctoral Dissertation]. University of Manchester; 2016. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:302324

University of Cape Town

16. Ambler, Jon Mitchell. Transcription analysis of virulent strains of Mycobacterium tuberculosis.

Degree: Image, Integrative Biomedical Sciences, 2018, University of Cape Town

URL: http://hdl.handle.net/11427/28434

► Background: Despite the development of new drugs and success of social programs, tuberculosis remains a leading cause of mortality. This burden falls disproportionately on developing… (more)

▼ Background: Despite the development of new drugs and success of social programs, tuberculosis remains a leading cause of mortality. This burden falls disproportionately on developing countries where the high burden of HIV has a potentiating e↵ect, but may soon return to areas where it was previously brought under control as resistant strains continue to emerge. In the Western Cape, two closely related strains of the Beijing family have been isolated that provide an opportunity to study virulence in a system with relatively little noise. The aim of this project was to identify the cause of the altered virulence displayed between the two strains, and describe how the di↵erences between the two genomes contributed to the phenotypic di↵erences. Results: GenGraph allows for the creation of graph genomes, and facilitated the creation of a pan-transcriptome that allowed for the mapping of gene annotations between isolates. This allowed for the mapping of reads to a more suitable Beijing family reference while interpreting the results with annotations from the H37Rv reference. We generated expression and target profiles for the known sRNA, and identified a large number of novel sRNA. Transcriptomic data from 4 di↵erent growth conditions was integrated with this sRNA data as well as variant data using the Cell pipeline. From this data we identified multiple sets of genes linked to copper sensing in MTB, including the di↵erentially expressed MoCo operon. Increasing evidence that macrophages use copper to poison bacteria trapped in their phagosomes provides the link to virulence and pathogenicity. Conclusions: Through the integration of data from multiple data types we were able to elucidate the most probable cause of the altered virulence found between the two isolates in this study. We developed reusable tools and pipelines, and noted a large number of undescribed sRNA expressed in these isolates. The identification of the copper response as a chief contributor to the phenotype increases both our understanding of the isolates, and the role of the element in infection. These results will be key in guiding further investigation of the variant linked genes to identify those linked to copper homeostasis or response. Advisors/Committee Members: Mulder, Nicola (advisor).

Subjects/Keywords: integrative biomedicine; mycobacterium tuberculosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ambler, J. M. (2018). Transcription analysis of virulent strains of Mycobacterium tuberculosis. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/28434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ambler, Jon Mitchell. “Transcription analysis of virulent strains of Mycobacterium tuberculosis.” 2018. Thesis, University of Cape Town. Accessed December 14, 2019. http://hdl.handle.net/11427/28434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ambler, Jon Mitchell. “Transcription analysis of virulent strains of Mycobacterium tuberculosis.” 2018. Web. 14 Dec 2019.

Vancouver:

Ambler JM. Transcription analysis of virulent strains of Mycobacterium tuberculosis. [Internet] [Thesis]. University of Cape Town; 2018. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/11427/28434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ambler JM. Transcription analysis of virulent strains of Mycobacterium tuberculosis. [Thesis]. University of Cape Town; 2018. Available from: http://hdl.handle.net/11427/28434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brunel University

17. Penny, Sarah. Sangoma Boy.

Degree: PhD, 2017, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/16048 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764888

► 'Sangoma Boy' is the first book in an intended four-book series. The series is about three children who are brought together because of their parents'… (more)

▼ 'Sangoma Boy' is the first book in an intended four-book series. The series is about three children who are brought together because of their parents' romantic involvement and who, by the end of the first book, come to identify themselves as 'The Bundu Bashers'. The idea for the Bundu Bashers series came to me when I was visiting at my parents' house in Cape Town, South Africa, in January, 2008. I had a newborn daughter who had to be breastfed every three hours as well as a ferociously jealous toddler son in tow, so my movements were pretty limited. It was a little difficult to go out to see childhood friends but lots of people dropped by to say hello and share a 'braai' (or barbecue) which is what you do just about every evening in the South African summer. One evening, one of these informal braai parties included Julia Raynham, my mother's goddaughter. I offered her some meat from the braai and she said yes, but not mutton because she was a sangoma now and there was a restriction on eating mutton. To say I was astonished is an understatement. A sangoma is a traditional African healer and diviner, versed in the arcana of plant medicine and spirit intercession. But Julia? My mother's goddaughter, no less? She was a whitey, just like me. We used to dive spoons together out of her parents' vast and twinkling swimming pool under the oak trees, in our walled-off privileged white childhood during apartheid. How could she possibly be a sangoma? Admittedly I'd been a bit busy in England with starting a career and squeezing out the babies, but the last time I saw Julia she was a keyboardist in a popular lounge band, The Honeymoon Suites, we used to go and bop to on Friday nights. I knew that she had been ill and I knew her long-term relationship had ended. But a sangoma? After a lengthy talk that night I understood a little better what Julia had been through since I saw her last. One doesn't chose to become a sangoma - one gets chosen. The first signs of being chosen are not pleasant, and usually include illness or affliction. With Julia, it was an onset of numbness in her legs, followed by a host of other complaints. Whilst in the grip of the illness, the sufferer sometimes has a vision, where they will be told to go to a certain house or place, or sometimes they just unexpectedly come into contact with a sangoma. Julia dreamt she needed to go to an art gallery in Johannesburg. At this stage she knew nothing about sangomas but the dream was so compelling that she did visit the gallery. At the gallery, a black cleaner put down her bucket and mop, walked over to Julia and asked her if she had been unwell recently, and experiencing peculiar dreams. This is the beginning of 'ukuthwasa' - the long period of training before the 'thwasa' (the initiate) emerges as a diviner. I mulled the whole thing over in the long night that followed while I was up feeding my daughter. So white people could be 'thwasana' now? That got me thinking. What if you were a person who was mixed-race, both white and black, with roots in Africa but living…

Subjects/Keywords: Western; Biomedicine; Ukuthwasa; Children's

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Penny, S. (2017). Sangoma Boy. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/16048 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764888

Chicago Manual of Style (16^th Edition):

Penny, Sarah. “Sangoma Boy.” 2017. Doctoral Dissertation, Brunel University. Accessed December 14, 2019. http://bura.brunel.ac.uk/handle/2438/16048 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764888.

MLA Handbook (7^th Edition):

Penny, Sarah. “Sangoma Boy.” 2017. Web. 14 Dec 2019.

Vancouver:

Penny S. Sangoma Boy. [Internet] [Doctoral dissertation]. Brunel University; 2017. [cited 2019 Dec 14]. Available from: http://bura.brunel.ac.uk/handle/2438/16048 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764888.

Council of Science Editors:

Penny S. Sangoma Boy. [Doctoral Dissertation]. Brunel University; 2017. Available from: http://bura.brunel.ac.uk/handle/2438/16048 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764888

18. Silva, Mônica Freitas da. Engenharia de superfície de nanopartículas magnéticas para biomedicina: recobrimentos com macromoléculas visando estabilização e compatibilidade em meio fisiológico.

Degree: Mestrado, Físico-Química, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/75/75134/tde-23042013-105323/ ;

►

Nanoparticulas magnéticas de óxido de ferro tem sido amplamente utilizadas em diversas áreas da biotecnologia e biomedicina, tais como no tratamento de câncer, marcação de… (more)

▼

Nanoparticulas magnéticas de óxido de ferro tem sido amplamente utilizadas em diversas áreas da biotecnologia e biomedicina, tais como no tratamento de câncer, marcação de célula e como agentes de contraste em imagem por ressonância magnética. O intuito deste trabalho foi sintetizar as nanopartículas magnéticas com magnetização de saturação intensificadas via processo do poliol modificado, e usando agentes de superfície para melhorar as propriedades de superfície. Carboximetildextrana, metilpolietilenoglicol (MPEG), quitosana, sílica e 3-aminopropiltrimetoxisilano (APTMS) foram utilizados para a modificação da superfície. Através da microscopia eletrônica de transmissão (TEM), foi obtido que as nanopartículas magnéticas de magnetita obtiveram um diâmetro médio de 5nm, em uma estreita distribuição de tamanho. A difração de raios-X (DRX) indicou a formação de magnetita em todos os sistemas em que o método do poliol modificado foi utilizado. As medidas de espectroscopia no infravermelho (FTIR) evidenciaram a presença de modos de vibração relacionados às macromoléculas e compostos inorgânicos utilizados na modificação de superfície das nanopartículas magnéticas. A TEM das diferentes modificações de superfície mostram a formação de aglomerados dependendo do modificador utilizado. As nanopartículas recobertas com APTMS foram funcionalizadas com ácido fólico, mostrando resultados satisfatórios, porém serão necessárias outras técnicas de caracterização. Para a funcionalização foi determinada a quantidade de amina livre na superfície da nanopartícula recoberta com APTMS e a técnica de UV-Vis determinou um bom resultado. A magnetometria de amostra vibrante (VSM) mostrou comportamentos semelhantes para todas as amostras recobertas em comparação a amostra sem recobrimento. Estes resultados evidenciam que a modificação de superfície foi realizada satisfatoriamente. Os métodos utilizados para realizar a mudança para hidrofóbica a superfície inicialmente hidrofílica se mostraram efetivos, porém a quantidade de agentes modificadores deve ser melhor estudada. Portanto, as nanopartículas magnéticas funcionalizadas com diferentes superfícies foram obtidas e possuem um alto potencial para serem utilizadas em aplicações em biomedicina.

Superparamagnetic iron oxides nanoparticles (SPION) have been highlighted in several areas of biotechnology and biomedicine, for example in cancer treatment, in labeling of cells and as contrast agent in magnetic resonance imaging (MRI). The purpose of this study was synthesizing SPION with intensified saturation magnetization by modified polyol process, and using surface agents to enhance the surface properties. Carboxymethildextran, metylpolietileneglycol, chitosan, silica and 3-aminopropyltrimethoxysilane (APTMS) were utilized as surface modifiers. By transmission electron microscopy (TEM), SPION showed narrow particle size distribution, with an average diameter around 5 nm. The X-ray diffraction studies indicated the formation of magnetite in all synthesized systems in which the modified polyol…

Advisors/Committee Members: Varanda, Laudemir Carlos.

Subjects/Keywords: biomedicina; biomedicine; macromoléculas; macromolecules; nanoparticles; nanopartículas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silva, M. F. d. (2013). Engenharia de superfície de nanopartículas magnéticas para biomedicina: recobrimentos com macromoléculas visando estabilização e compatibilidade em meio fisiológico. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75134/tde-23042013-105323/ ;

Chicago Manual of Style (16^th Edition):

Silva, Mônica Freitas da. “Engenharia de superfície de nanopartículas magnéticas para biomedicina: recobrimentos com macromoléculas visando estabilização e compatibilidade em meio fisiológico.” 2013. Masters Thesis, University of São Paulo. Accessed December 14, 2019. http://www.teses.usp.br/teses/disponiveis/75/75134/tde-23042013-105323/ ;.

MLA Handbook (7^th Edition):

Silva, Mônica Freitas da. “Engenharia de superfície de nanopartículas magnéticas para biomedicina: recobrimentos com macromoléculas visando estabilização e compatibilidade em meio fisiológico.” 2013. Web. 14 Dec 2019.

Vancouver:

Silva MFd. Engenharia de superfície de nanopartículas magnéticas para biomedicina: recobrimentos com macromoléculas visando estabilização e compatibilidade em meio fisiológico. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2019 Dec 14]. Available from: http://www.teses.usp.br/teses/disponiveis/75/75134/tde-23042013-105323/ ;.

Council of Science Editors:

Silva MFd. Engenharia de superfície de nanopartículas magnéticas para biomedicina: recobrimentos com macromoléculas visando estabilização e compatibilidade em meio fisiológico. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/75/75134/tde-23042013-105323/ ;

University of Illinois – Chicago

19. Habans, Robert A. The Urban Biomedical District: Health Care and Economic Development in American Cities.

Degree: 2017, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/21783

► To date, little scholarship has directly examined health care as both a distinctive, complex institution and a motive force in the economies of cities. This… (more)

Subjects/Keywords: urban planning; biomedicine; economic development; health care

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Habans, R. A. (2017). The Urban Biomedical District: Health Care and Economic Development in American Cities. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21783

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Habans, Robert A. “The Urban Biomedical District: Health Care and Economic Development in American Cities.” 2017. Thesis, University of Illinois – Chicago. Accessed December 14, 2019. http://hdl.handle.net/10027/21783.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Habans, Robert A. “The Urban Biomedical District: Health Care and Economic Development in American Cities.” 2017. Web. 14 Dec 2019.

Vancouver:

Habans RA. The Urban Biomedical District: Health Care and Economic Development in American Cities. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10027/21783.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Habans RA. The Urban Biomedical District: Health Care and Economic Development in American Cities. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21783

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hawaii – Manoa

20. Vaughan, Ashley Marie. The gift and the road : exploring the meanings of health and illness in Tautu, Vanuatu.

Degree: 2016, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100762

►

Ph.D. University of Hawaii at Manoa 2013.

Based on 13 months of ethnographic research, this dissertation documents how the people of Tautu, Vanuatu incorporate the… (more)

▼

Ph.D. University of Hawaii at Manoa 2013.

Based on 13 months of ethnographic research, this dissertation documents how the people of Tautu, Vanuatu incorporate the global flows of biomedicine and Christianity into their local knowledge structures and social practices and the resulting integration of biomedical, Christian, and traditional medical ideologies and practices. This integration is articulated in Tautuans' theories of illness causation and categories of care; knowledge practice; diagnosis and treatment processes; definitions of health and illness; and healing narratives. At the center of this creative, integrative process are traditional social relations based on kinship and exchange and the related principles of pragmatism, "dividuality," and reciprocity. However, Christianity – specifically "gift" narratives in which Tautuans explain that their healing knowledge and powers come from God – is the main discursive frame through which Tautuans create a middle ground between "traditional" and "modern/Western." These gift narratives serve multiple purposes. First, they allow patients and practitioners to organize their past experiences and to make sense of suffering. Second, in these "gift" narratives Tautuans authorize certain aspects of traditional medicine by reconstructing them as Christian knowledge. Third, through these narratives Tautuans are carving out a space where biomedical, traditional, Christian forms of healing are complimentary practices, as the idea that all types of medicine are "gifts from God" causes the categories of biomedical, traditional, and Christian to fall away and to be subsumed by the larger heading of "spiritual healing." Fourth, these gift narratives are also religious narratives about salvation intended to convert people not only to kastom medicine but also to the Christian faith. Finally, these narratives are an attempt to appropriate and indigenize biomedical ideologies and forms of knowledge production and to gain international recognition of the efficacy of traditional medicinal plants; these narratives, then, illustrate Tautuans' desires to globalize their local practices and to engage with the modern world on their own terms.

Subjects/Keywords: ethnographic research; Tautu; Vanuatu; biomedicine; Christianity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaughan, A. M. (2016). The gift and the road : exploring the meanings of health and illness in Tautu, Vanuatu. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100762

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vaughan, Ashley Marie. “The gift and the road : exploring the meanings of health and illness in Tautu, Vanuatu.” 2016. Thesis, University of Hawaii – Manoa. Accessed December 14, 2019. http://hdl.handle.net/10125/100762.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vaughan, Ashley Marie. “The gift and the road : exploring the meanings of health and illness in Tautu, Vanuatu.” 2016. Web. 14 Dec 2019.

Vancouver:

Vaughan AM. The gift and the road : exploring the meanings of health and illness in Tautu, Vanuatu. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10125/100762.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vaughan AM. The gift and the road : exploring the meanings of health and illness in Tautu, Vanuatu. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/100762

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

21. Tabakovic, Amra. Investigation of Laundering and Dispersion Approaches for Silica and Calcium Phosphosilicate Nanoparticles Synthesized in Reverse Micelles.

Degree: PhD, Materials Science and Engineering, 2015, Penn State University

URL: https://etda.libraries.psu.edu/catalog/26559

► Nanotechnology, the science and engineering of materials at the nanoscale, is a booming research area with numerous applications in electronic, cosmetic, automotive and sporting goods… (more)

▼ Nanotechnology, the science and engineering of materials at the nanoscale, is a booming research area with numerous applications in electronic, cosmetic, automotive and sporting goods industries, as well as in biomedicine. Composite nanoparticles (NPs) are of special interest since the use of two or more materials in NP design imparts multifunctionality on the final NP constructs. This is especially relevant for applications in areas of human healthcare, where the use of dye or drug doped composite NPs is expected to improve the diagnosis and treatment of cancer and other serious illnesses. Since the physicochemical properties of NP suspensions dictate the success of these systems in biomedical applications, especially drug delivery of chemotherapeutics, synthetic routes which offer precise control of NP properties, especially particle diameter and colloidal stability, are utilized to form a variety of composite NPs. Formation of NPs in reverse, or water-in-oil, micelles is one such synthetic approach. However, while the use of reverse micelles to form composite NPs offers precise control over NP size and shape, the post-synthesis laundering and dispersion of synthesized NP suspensions can still be a challenge. Reverse micelle synthetic approaches require the use of surfactants and low dielectric constant solvents, like hexane and cyclohexane, as the oil phase, which can compromise the biocompatibility and colloidal stability of the final composite NP suspensions. Therefore, appropriate dispersants and solvents must be used during laundering and dispersion to remove surfactant and ensure stability of synthesized NPs. In the work presented in this dissertation, two laundering and dispersion approaches, including packed column high performance liquid chromatography (HPLC) and centrifugation (sedimentation and redispersion), are investigated for silver core silica (Ag-SiO2) and calcium phosphosilicate (Caw(HxPO4)y(Si(OH)zOa)b.cH2O, CPS) composite NP suspensions synthesized in a cyclohexane/ polyoxyethylene (5) nonylphenylether (Igepal® CO-520) /water reverse micelle system. In Chapter 3, an HPLC laundering and dispersion approach for Ag-SiO2 composite NPs is studied. The deposition and detachment behavior of 5 w/w 3- aminopropyltriethoxy silane (APTES) dispersed Ag-SiO2 suspensions on 1 w/o, 5 w/o and 15 w/o APTES treated SiO2 column stationary phase microspheres is investigated with respect to final % mass yields for collected NP suspensions. Field emission scanning electron microscopy (FESEM) studies confirm multilayer deposition of Ag-SiO2 NPs during loading and retention on the column during laundering stages of the process independent of w/o APTES treatment on stationary phase microspheres, with individual deposited Ag-SiO2 NPs observed to be in the 20-30 nm diameter range. Patchy deposition patterns and irreversible agglomeration are also confirmed via FESEM, as fractions of loaded Ag-SiO2 NPs remain attached on the 1 w/o, 5 w/o and 15 w/o APTES stationary phases after completion of the collecting stage of the…

Subjects/Keywords: Nanoparticles; nanoparticle laundering; nanotechnology; biomedicine; drug delivery

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tabakovic, A. (2015). Investigation of Laundering and Dispersion Approaches for Silica and Calcium Phosphosilicate Nanoparticles Synthesized in Reverse Micelles. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/26559

Chicago Manual of Style (16^th Edition):

Tabakovic, Amra. “Investigation of Laundering and Dispersion Approaches for Silica and Calcium Phosphosilicate Nanoparticles Synthesized in Reverse Micelles.” 2015. Doctoral Dissertation, Penn State University. Accessed December 14, 2019. https://etda.libraries.psu.edu/catalog/26559.

MLA Handbook (7^th Edition):

Tabakovic, Amra. “Investigation of Laundering and Dispersion Approaches for Silica and Calcium Phosphosilicate Nanoparticles Synthesized in Reverse Micelles.” 2015. Web. 14 Dec 2019.

Vancouver:

Tabakovic A. Investigation of Laundering and Dispersion Approaches for Silica and Calcium Phosphosilicate Nanoparticles Synthesized in Reverse Micelles. [Internet] [Doctoral dissertation]. Penn State University; 2015. [cited 2019 Dec 14]. Available from: https://etda.libraries.psu.edu/catalog/26559.

Council of Science Editors:

Tabakovic A. Investigation of Laundering and Dispersion Approaches for Silica and Calcium Phosphosilicate Nanoparticles Synthesized in Reverse Micelles. [Doctoral Dissertation]. Penn State University; 2015. Available from: https://etda.libraries.psu.edu/catalog/26559

Clemson University

22. Fellows, Benjamin David. Synthesis, Biofunctionalization, and Application of Magnetic Nanomaterials.

Degree: PhD, School of Materials Science and Engineering, 2018, Clemson University

URL: https://tigerprints.clemson.edu/all_dissertations/2221

► Since their inception in the late 1970's magnetic nanomaterials have sparked heavy research into their use in the biomedical field. Their unique magnetic properties allow… (more)

▼ Since their inception in the late 1970's magnetic nanomaterials have sparked heavy research into their use in the biomedical field. Their unique magnetic properties allow the magnetic particles to be the base for a large array of expiremental medical techniques, from treatments of disease, diagnostic tests, imaging aids, and more. In this manuscript, each stage starting from novel particle synthesis, functionalization with bioactive molecules, and innovative application is explored, specifically using the techniques magnetically mediated energy delivery, magnetophoresis, magnetic resonance imaging. The reproducible synthesis of nanomaterials is necessary if any further engineering application is going to be done. Using a novel extended LaMer approach where a precursor solution is consistently added to a reaction vessel allows for the linear volume growth of nanoparticles. This technique was originally used for the synthesis of magnetite (a simple ferrite) to control the volume of the particle indefinitely. Transferring it to a nonstoichiometric cobalt ferrite, it is shown that a linear volume growth is achieved up to 20nm. Secondary functionality of the magnetic particles has really opened up the application to sensing, selective treatment, and functional imaging. Surface modification with a bacterial strain discriminatory glycan allows for strain selective treatment of bacterial infection. Heparin functional particles show high pharmacokinetic activity for the treatment of neointimal hyperplasia due to high surface area to volume ratios. Gadolinium coated particles have distance dependent effects on the MRI relaxation rate of water, which may prove useful in functional imaging. Each of these complexes shows promise as a new way to treat or image malady. Although a small part in the large picture in developing a new generation of medicine, this research lays the foundation for each of these possible treatments. Be it the eradication of bacterial infection or the non-toxic prevention of restenosis, novel multifunctional nanomaterials such as the ones discussed in this manuscript, will be heavily relied on in the future of medicine. Advisors/Committee Members: Dr. Olin Thompson Mefford, Committee Chair, Dr. Igor Luzinov, Dr. Marek Urban, Dr. Delphine Dean.

Subjects/Keywords: Biomagnetics; Biomedicine; Imaging; Magnetite; Nanoparticles; Synthesis

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APA (6^th Edition):

Fellows, B. D. (2018). Synthesis, Biofunctionalization, and Application of Magnetic Nanomaterials. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/2221

Chicago Manual of Style (16^th Edition):

Fellows, Benjamin David. “Synthesis, Biofunctionalization, and Application of Magnetic Nanomaterials.” 2018. Doctoral Dissertation, Clemson University. Accessed December 14, 2019. https://tigerprints.clemson.edu/all_dissertations/2221.

MLA Handbook (7^th Edition):

Fellows, Benjamin David. “Synthesis, Biofunctionalization, and Application of Magnetic Nanomaterials.” 2018. Web. 14 Dec 2019.

Vancouver:

Fellows BD. Synthesis, Biofunctionalization, and Application of Magnetic Nanomaterials. [Internet] [Doctoral dissertation]. Clemson University; 2018. [cited 2019 Dec 14]. Available from: https://tigerprints.clemson.edu/all_dissertations/2221.

Council of Science Editors:

Fellows BD. Synthesis, Biofunctionalization, and Application of Magnetic Nanomaterials. [Doctoral Dissertation]. Clemson University; 2018. Available from: https://tigerprints.clemson.edu/all_dissertations/2221

Clemson University

23. Wang, Bo. Understanding the Structure-Function Relationships of Dendrimers in Environmental and Biomedical Applications.

Degree: PhD, Physics and Astronomy, 2017, Clemson University

URL: https://tigerprints.clemson.edu/all_dissertations/1967

► We are living an era wherein nanoparticles (NPs) have been widely applied in our lives. Dendrimers are special polymeric NPs with unique physiochemical properties, which… (more)

▼ We are living an era wherein nanoparticles (NPs) have been widely applied in our lives. Dendrimers are special polymeric NPs with unique physiochemical properties, which have been intensely explored for a variety of applications. Current studies on dendrimers are bottlenecked by insufficient understandings of their structure and dynamic behaviors from a molecular level. With primarily computational approaches supplemented by many other experimental technics, this dissertation aims to establish structure-function relationships of dendrimers in environmental and biomedical applications. More specifically, it thoroughly investigates the interactions between dendrimers and different biomolecules including carbon-based NPs, metal-based NPs, and proteins/peptides. Those results not only provide profound knowledge for evaluating the impacts of dendrimers on environmental and biological systems but also facilitate designing next-generation functional polymeric nanomaterials. The dissertation is organized as following. Chapter 1 provides an overview of current progresses on dendrimer studies, where methodology of Discrete Molecular Dynamics (DMD), my major research tool, is also introduced. Two directions of utilizing dendrimers will be discussed in following chapters. Chapter 2 will focus on environmental applications of dendrimers, where two back-to-back studies are presented. I will start from describing some interesting observations from experiments i.e. dendrimers dispersed model oil molecules. Then, I will reveal why surface chemistries of dendrimers lead to different remediation efficiencies by computational modelings. Finally, I will demonstrate different scenarios of dendrimer-small molecules association. Chapter 3 is centered on dendrimers in the biomedical applications including two subtopics. In the first topic, we will discuss dendrimers as surfactants that modulating the interactions between proteins and NPs. Some fundamental concepts regarding to NPs-Protein interactions such as NP-protein corona are also explained. In the following topic, I will look into amyloid protein aggregation mediated by dendrimers, which is of high expectations for combating amyloidogenic-related diseases. Chapter 4 concludes the whole dissertation. It also briefly introduces my ongoing projects and future research directions about dendrimers. This dissertation has presented a systematic study of dendrimers in environmental and biomedical applications which might provide valuable information for future dendrimer design thus benefit the nanobiotechnology. Advisors/Committee Members: Dr. Feng Ding, Committee Chair, Dr. Emil Alexov, Dr. David A. Lander, Dr. Jian He.

Subjects/Keywords: biomedicine; biophysics; molecular simulation; nanoparticle; protein aggregation

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APA (6^th Edition):

Wang, B. (2017). Understanding the Structure-Function Relationships of Dendrimers in Environmental and Biomedical Applications. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/1967

Chicago Manual of Style (16^th Edition):

Wang, Bo. “Understanding the Structure-Function Relationships of Dendrimers in Environmental and Biomedical Applications.” 2017. Doctoral Dissertation, Clemson University. Accessed December 14, 2019. https://tigerprints.clemson.edu/all_dissertations/1967.

MLA Handbook (7^th Edition):

Wang, Bo. “Understanding the Structure-Function Relationships of Dendrimers in Environmental and Biomedical Applications.” 2017. Web. 14 Dec 2019.

Vancouver:

Wang B. Understanding the Structure-Function Relationships of Dendrimers in Environmental and Biomedical Applications. [Internet] [Doctoral dissertation]. Clemson University; 2017. [cited 2019 Dec 14]. Available from: https://tigerprints.clemson.edu/all_dissertations/1967.

Council of Science Editors:

Wang B. Understanding the Structure-Function Relationships of Dendrimers in Environmental and Biomedical Applications. [Doctoral Dissertation]. Clemson University; 2017. Available from: https://tigerprints.clemson.edu/all_dissertations/1967

University of Melbourne

24. Björnmalm, Axel Mattias Håkansson. Particles under flow: engineering and evaluating nanostructured particles in fluidic devices.

Degree: 2016, University of Melbourne

URL: http://hdl.handle.net/11343/114596

► Nanoengineered particles can be used to improve human health, in areas ranging from diagnostics and imaging to therapeutics and regenerative medicine, but only a few… (more)

Subjects/Keywords: materials science; microfluidics; nanomaterials; engineering; biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Björnmalm, A. M. H. (2016). Particles under flow: engineering and evaluating nanostructured particles in fluidic devices. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/114596

Chicago Manual of Style (16^th Edition):

Björnmalm, Axel Mattias Håkansson. “Particles under flow: engineering and evaluating nanostructured particles in fluidic devices.” 2016. Doctoral Dissertation, University of Melbourne. Accessed December 14, 2019. http://hdl.handle.net/11343/114596.

MLA Handbook (7^th Edition):

Björnmalm, Axel Mattias Håkansson. “Particles under flow: engineering and evaluating nanostructured particles in fluidic devices.” 2016. Web. 14 Dec 2019.

Vancouver:

Björnmalm AMH. Particles under flow: engineering and evaluating nanostructured particles in fluidic devices. [Internet] [Doctoral dissertation]. University of Melbourne; 2016. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/11343/114596.

Council of Science Editors:

Björnmalm AMH. Particles under flow: engineering and evaluating nanostructured particles in fluidic devices. [Doctoral Dissertation]. University of Melbourne; 2016. Available from: http://hdl.handle.net/11343/114596

Deakin University

25. Li, Rui. Designing self-assembled multicomponent scaffolds for regenerative medicine.

Degree: School of Life and Environmental Sciences, 2016, Deakin University

URL: http://hdl.handle.net/10536/DRO/DU:30089380

► This project demonstrated novel approaches to incorporate multiple bioactive peptide sequences polysaccharides and proteoglycans. Demonstrated applications for both in vitro 3D cell culture and in… (more)

Subjects/Keywords: Biomedicine; Biomaterials; Tissue engineering; Therapeutic medicine; Peptides

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APA (6^th Edition):

Li, R. (2016). Designing self-assembled multicomponent scaffolds for regenerative medicine. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30089380

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Rui. “Designing self-assembled multicomponent scaffolds for regenerative medicine.” 2016. Thesis, Deakin University. Accessed December 14, 2019. http://hdl.handle.net/10536/DRO/DU:30089380.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Rui. “Designing self-assembled multicomponent scaffolds for regenerative medicine.” 2016. Web. 14 Dec 2019.

Vancouver:

Li R. Designing self-assembled multicomponent scaffolds for regenerative medicine. [Internet] [Thesis]. Deakin University; 2016. [cited 2019 Dec 14]. Available from: http://hdl.handle.net/10536/DRO/DU:30089380.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li R. Designing self-assembled multicomponent scaffolds for regenerative medicine. [Thesis]. Deakin University; 2016. Available from: http://hdl.handle.net/10536/DRO/DU:30089380

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

26. Porter, Sandra. An exploration of the support needs of ambulance paramedics.

Degree: phd, College of Health and Biomedicine, 2013, Victoria University

URL: http://vuir.vu.edu.au/22296/

► The work of ambulance paramedics is usually physically and emotionally draining and can place significant amounts of pressure on the emergency service worker. The work… (more)

Subjects/Keywords: 1701 Psychology; College of Health and Biomedicine

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APA (6^th Edition):

Porter, S. (2013). An exploration of the support needs of ambulance paramedics. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/22296/

Chicago Manual of Style (16^th Edition):

Porter, Sandra. “An exploration of the support needs of ambulance paramedics.” 2013. Doctoral Dissertation, Victoria University. Accessed December 14, 2019. http://vuir.vu.edu.au/22296/.

MLA Handbook (7^th Edition):

Porter, Sandra. “An exploration of the support needs of ambulance paramedics.” 2013. Web. 14 Dec 2019.

Vancouver:

Porter S. An exploration of the support needs of ambulance paramedics. [Internet] [Doctoral dissertation]. Victoria University; 2013. [cited 2019 Dec 14]. Available from: http://vuir.vu.edu.au/22296/.

Council of Science Editors:

Porter S. An exploration of the support needs of ambulance paramedics. [Doctoral Dissertation]. Victoria University; 2013. Available from: http://vuir.vu.edu.au/22296/

Victoria University

27. Kruger, Gina. Promoting normal birthing : aspiring to develop a midwife-led scope of practice.

Degree: phd, College of Health and Biomedicine, 2014, Victoria University

URL: http://vuir.vu.edu.au/24842/

► The purpose of this study was to develop a substantive theory of the processes used by midwives in exercising their scope of practice when caring… (more)

Subjects/Keywords: 1110 Nursing; College of Health and Biomedicine

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APA (6^th Edition):

Kruger, G. (2014). Promoting normal birthing : aspiring to develop a midwife-led scope of practice. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/24842/

Chicago Manual of Style (16^th Edition):

Kruger, Gina. “Promoting normal birthing : aspiring to develop a midwife-led scope of practice.” 2014. Doctoral Dissertation, Victoria University. Accessed December 14, 2019. http://vuir.vu.edu.au/24842/.

MLA Handbook (7^th Edition):

Kruger, Gina. “Promoting normal birthing : aspiring to develop a midwife-led scope of practice.” 2014. Web. 14 Dec 2019.

Vancouver:

Kruger G. Promoting normal birthing : aspiring to develop a midwife-led scope of practice. [Internet] [Doctoral dissertation]. Victoria University; 2014. [cited 2019 Dec 14]. Available from: http://vuir.vu.edu.au/24842/.

Council of Science Editors:

Kruger G. Promoting normal birthing : aspiring to develop a midwife-led scope of practice. [Doctoral Dissertation]. Victoria University; 2014. Available from: http://vuir.vu.edu.au/24842/

Victoria University

28. Liu, Sarah. The impact of borderline personality traits on commitment in romantic relationships: An application of the investment model.

Degree: other, College of Health and Biomedicine, 2017, Victoria University

URL: http://vuir.vu.edu.au/34438/

► Borderline personality disorder is characterised by marked impairment in affective, cognitive, behavioural and interpersonal functioning. Recognising the growing need to understand personality pathology on a… (more)

Subjects/Keywords: 1701 Psychology; College of Health and Biomedicine

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APA (6^th Edition):

Liu, S. (2017). The impact of borderline personality traits on commitment in romantic relationships: An application of the investment model. (Thesis). Victoria University. Retrieved from http://vuir.vu.edu.au/34438/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Sarah. “The impact of borderline personality traits on commitment in romantic relationships: An application of the investment model.” 2017. Thesis, Victoria University. Accessed December 14, 2019. http://vuir.vu.edu.au/34438/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Sarah. “The impact of borderline personality traits on commitment in romantic relationships: An application of the investment model.” 2017. Web. 14 Dec 2019.

Vancouver:

Liu S. The impact of borderline personality traits on commitment in romantic relationships: An application of the investment model. [Internet] [Thesis]. Victoria University; 2017. [cited 2019 Dec 14]. Available from: http://vuir.vu.edu.au/34438/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu S. The impact of borderline personality traits on commitment in romantic relationships: An application of the investment model. [Thesis]. Victoria University; 2017. Available from: http://vuir.vu.edu.au/34438/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

29. Trpcevska, Lidija. Predictors of psychological well-being, academic self-efficacy and resilience in university students, and their impact on academic motivation.

Degree: other, College of Health and Biomedicine, 2017, Victoria University

URL: http://vuir.vu.edu.au/34676/

► Compared to the general population, higher education students report elevated levels of psychological distress, with symptoms of and criteria for serious mental illness also being… (more)

Subjects/Keywords: 1701 Psychology; College of Health and Biomedicine

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APA (6^th Edition):

Trpcevska, L. (2017). Predictors of psychological well-being, academic self-efficacy and resilience in university students, and their impact on academic motivation. (Thesis). Victoria University. Retrieved from http://vuir.vu.edu.au/34676/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Trpcevska, Lidija. “Predictors of psychological well-being, academic self-efficacy and resilience in university students, and their impact on academic motivation.” 2017. Thesis, Victoria University. Accessed December 14, 2019. http://vuir.vu.edu.au/34676/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Trpcevska, Lidija. “Predictors of psychological well-being, academic self-efficacy and resilience in university students, and their impact on academic motivation.” 2017. Web. 14 Dec 2019.

Vancouver:

Trpcevska L. Predictors of psychological well-being, academic self-efficacy and resilience in university students, and their impact on academic motivation. [Internet] [Thesis]. Victoria University; 2017. [cited 2019 Dec 14]. Available from: http://vuir.vu.edu.au/34676/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trpcevska L. Predictors of psychological well-being, academic self-efficacy and resilience in university students, and their impact on academic motivation. [Thesis]. Victoria University; 2017. Available from: http://vuir.vu.edu.au/34676/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

30. Quinn, Melanie. Psychosocial Outcomes and Predictors of Distress Among Military Spouses.

Degree: other, College of Health and Biomedicine, 2017, Victoria University

URL: http://vuir.vu.edu.au/35043/

► The current research aimed to further investigate the nature and extent of psychological distress among Australian Defence Force spouses, predictors of such distress, and the… (more)

▼ The current research aimed to further investigate the nature and extent of psychological distress among Australian Defence Force spouses, predictors of such distress, and the protective factors that moderate these negative effects, using internationally validated methods. This study used Lazarus and Folkman’s (1984) theory of stress and coping as an overarching theoretical framework. A sample of spouses (n = 184) completed an online self-report instrument that assessed psychological distress, depression, anxiety and stress, quality of sleep, barriers to care, and levels of perceived social support. Consistent with hypotheses, multiple regression analyses showed that military spouses reported significantly higher rates of distress and significantly poorer sleep quality than rates reported in the general Australian population. Higher rates of depression were predicted by greater discrepancy between emotional support received than was desired, greater discrepancy between practical support received than was desired, and current deployment status of the service partner. High rates of anxiety were predicted by the total number of barriers to care endorsed by spouses. Poorer sleep quality was predicted by more discrepancy between practical support received than was desired. Contrary to hypotheses, military risk factors of the duration of service for the ADF partner and amount of times deployed did not predict psychological distress. Similarly, barriers to care and self-stigma of help-seeking did not moderate the association between the discrepancy between emotional support received than was desired and psychological distress outcomes. Findings indicated that military spouses are a vulnerable population group with high rates of distress and sleep difficulties. Spouses are impacted by stressors such as the current deployment of the service partner and help-seeking efforts are impeded by deficient access to supports, and barriers to care. Clinical implications are discussed, including the need to design appropriate interventions to specifically address the psychological implications of the demands placed on military spouses. – – - Running title: Psychosocial outcomes of miltary spouses

Subjects/Keywords: 1701 Psychology; College of Health and Biomedicine

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APA (6^th Edition):

Quinn, M. (2017). Psychosocial Outcomes and Predictors of Distress Among Military Spouses. (Thesis). Victoria University. Retrieved from http://vuir.vu.edu.au/35043/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Quinn, Melanie. “Psychosocial Outcomes and Predictors of Distress Among Military Spouses.” 2017. Thesis, Victoria University. Accessed December 14, 2019. http://vuir.vu.edu.au/35043/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Quinn, Melanie. “Psychosocial Outcomes and Predictors of Distress Among Military Spouses.” 2017. Web. 14 Dec 2019.

Vancouver:

Quinn M. Psychosocial Outcomes and Predictors of Distress Among Military Spouses. [Internet] [Thesis]. Victoria University; 2017. [cited 2019 Dec 14]. Available from: http://vuir.vu.edu.au/35043/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Quinn M. Psychosocial Outcomes and Predictors of Distress Among Military Spouses. [Thesis]. Victoria University; 2017. Available from: http://vuir.vu.edu.au/35043/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations