subject:(Biology AND Life Sciences)

Johannes Gutenberg Universität Mainz

1. Hussein Allami, Risala. Influence of the chemokine CXCL12 on the progression and the signaling in colorectal cancer.

Degree: 2013, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2013/3489/

► Das Chemokin CXCL12 (auch bekannt als SDF-1) ist ein kleines Protein (8-14) KDa, das in sechs Isoformen exprimiert wird (SDF-1α, SDF-1β, SDF-1γ, SDF- 1δ, SDF-1ε… (more)

▼ Das Chemokin CXCL12 (auch bekannt als SDF-1) ist ein kleines Protein (8-14) KDa, das in sechs Isoformen exprimiert wird (SDF-1α, SDF-1β, SDF-1γ, SDF- 1δ, SDF-1ε und SDF-1θ) von einem einzigen Gen, dass die Leukozyten-Wanderung regelt und variabel in einer Reihe von normalen und Krebsgeweben exprimiert wird.rnCXCL12 spielt verschiedene Rollen in der Tumorpathogenese. Es wurde nachgewiesen, dass CXCL12 das Tumorwachstum und die Malignität fördert, die Tumorangiogenese stärkt, sich an der Metastasierung beteiligt und zu immunsuppressiven Netzwerken innerhalb des Tumormikromilieus beiträgt. Daher liegt es nahe, dass der CXCL12/CXCR4-Signalweg ein wichtiges Ziel ist für die Entwicklung von neuartigen Krebstherapien.rnUm Licht auf die Rolle der Chemokin CXCL12 Splicevarianten in der Entwicklung von Krebs zu werfen und die mögliche physiologische Relevanz und ihre möglichen funktionellen Unterschiede bei Darmkrebs zu verstehen, haben wir alle CXCL12 Splicevarianten (alpha, beta, gamma, delta, epsilon und theta) in die kolorektalen Zelllinie SW480 und die Melanomzellinie D05 transfiziert und exprimiert.rnrnDiese Arbeit wurde erstellt, um die folgenden Ziele zu erreichen. Untersuchung der Rolle von CXCL12 Splicevarianten bei der Vermittlung von Tumorprogression, Adhäsion, Migration, Invasion und Metastasierung von Darmkrebs. Untersuchung, ob die CXCL12 Variantenwege ein wichtiges Ziel für die Entwicklung von Krebstherapien darstellen.rn• Um eine in vivo Mausmodell zu entwickeln, um die Rolle der CXCL12 Varianten im Rahmen des Tumorwachstums zu verstehen.rnrnUnsere Ergebnisse zeigen, dass:Der CXCL12 G801A Polymorphismus ist ein Low-Penetranz Risikofaktor für die Entwicklung von Darmkrebs. Der CXCL12-Gen-Polymorphismus rs1801157 ist mit dem T-Status (Tumor-node-Metastasen) assoziiert. Es gab keine Beziehung zwischen CXCL12-Gen-Polymorphismus rs1801157 und Fernmetastisen oder LN metastasen. Alle sechs CXCL12 Splicevarianten werden im Darmkrebs und in gesunder Kolon mucosa exprimiert. Die höchste Expression wird bei SDF-1alpha, dann SDF-1 beta gefunden. Alle sechs CXCL12 Varianten zeigen erhöhte Tumorzellproliferation in vitro. SDF-1beta, gefolgt von SDF-1alpha zeigte die größte Aktivität im Proliferationsassay.rn• Alle sechs CXCL12 Varianten induzieren die Tumorzelladhäsion.SDF-1beta dann SDF-1alpha zeigte die größte Aktivität im Rahmen des Adhäsionsassay. Alle sechs CXCL12 Varianten erhöhten die Zellmigration und Invasion von Tumorzellen in vitro. SDF-1theta und SDF-1epsilon 1theta zeigten die größte Aktivität, während die schwächste Aktivität mit SDF-1alpha und SDF-1beta beobachtet wurde. Alle sechs CXCL12 Varianten aktivieren Akt und (MAPK) Mitogen- acktivatedierte Protein kinase Wege und damit die Regulierung viele essentieller Prozesse in Tumorzellen, wie Proliferation, Migration, Invasion und Adhäsion. Es ist interessant festzustellen, dass AMD3100 die CXCL12 Splicevarianten inhibriert, die AKT-MEK-1/2-Phosphorylierung induzieren.rnDer Inhibitor AMD3100 unterdrückt stark die CXCL12 Varianten -delta, -epsilon und…

Subjects/Keywords: Biologie; Life sciences

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APA (6^th Edition):

Hussein Allami, R. (2013). Influence of the chemokine CXCL12 on the progression and the signaling in colorectal cancer. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2013/3489/

Chicago Manual of Style (16^th Edition):

Hussein Allami, Risala. “Influence of the chemokine CXCL12 on the progression and the signaling in colorectal cancer.” 2013. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 25, 2020. http://ubm.opus.hbz-nrw.de/volltexte/2013/3489/.

MLA Handbook (7^th Edition):

Hussein Allami, Risala. “Influence of the chemokine CXCL12 on the progression and the signaling in colorectal cancer.” 2013. Web. 25 Jan 2020.

Vancouver:

Hussein Allami R. Influence of the chemokine CXCL12 on the progression and the signaling in colorectal cancer. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2013. [cited 2020 Jan 25]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2013/3489/.

Council of Science Editors:

Hussein Allami R. Influence of the chemokine CXCL12 on the progression and the signaling in colorectal cancer. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2013. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2013/3489/

Universiteit Utrecht

2. Hofman, E.G. Plasma membrane organization during EGFR signaling: a FRET-based analysis.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/30336

► Since two decades it has been suggested that the plasma membrane is organized into lipid-separated domains called lipid rafts. A number of functions have been… (more)

▼ Since two decades it has been suggested that the plasma membrane is organized into lipid-separated domains called lipid rafts. A number of functions have been attributed to these domains, including spatially separating or combining functionally linked proteins. Proteins such as the Epidermal Growth Factor Receptor (EGFR) are found to be localized in membrane domains, as well as effector molecules downstream in the signal transduction cascade. The investigation of these structures has mainly been performed by highly invasive techniques such as biochemical analysis, but lacks data on the situation in intact cells. Chapter 2 of this thesis describes the use of Förster Resonance Energy Transfer (FRET) to study the presence and dynamics of membrane domains containing EGFR. To study this receptor we developed fluorescent nanobodies, monovalent domains from cameloid heavy-chain only antibodies. FRET-FLIM analysis revealed that EGFR resides in a subclass of nanodomains including the ganglioside GM1, and is absent in domains composed of GM1 and GPI-linked GFP. Activation of EGFR results in the coalescence of the two domains, suggesting the formation of signaling platforms. Lipid domains are often suggested to promote the local clustering of their constituents. Therefore, a part of this work describes the development of a novel approach to study the oligomerization state of the domain components in intact cells. This technique, confocal time-resolved fluorescence anisotropy imaging microscopy (CTRFAIM), is based on FRET between identical fluorophores (homo-FRET). The anisotropy, defined as the degree of polarization of the fluorescence, is directly related to the degree of oligomerization. In this thesis, a simplification of this approach is described by applying time-gated data acquisition (chapter 3 and 4). An inducible FKBP dimerization and oligomerization system was developed to correlate the anisotropy value to the oligomerization state. Chapter 4 describes an evaluation of different modes of data acquisition. When compared to steady-state anisotropy, CTR-FAIM shows an improved dynamic range of anisotropy values. A further improvement can be obtained with two-photon excitation, although this improvement is diminished by significant higher photobleaching (chapter 4). When subjected to CTRFAIM analysis, the domain components in our study were indeed found to be clustered. By controlled photobleaching, the lipid raft probe GPI-GFP was found to form small nanoclusters of 1-5 molecules. Also EGFR was found to organize into oligomers, depending on the activation state of the receptor. The CTRFAIM data reveal pre-existing dimers of EGFR in the plasma membrane of resting cells. After stimulation with EGF, the receptor oligomerizes in a kinase- and phosphotyrosine-dependent manner, forming clusters of 3 or more receptors (chapter 5). Furthermore, induced receptor clustering enhances receptor internalization speed, which suggests a stimulatory role for EGFR oligomerization in the internalization process. In conclusion, these data… Advisors/Committee Members: Verkleij, A, van Bergen en Henegouwen, P.

Subjects/Keywords: Molecular biology; Life sciences; Cell biology; Biologie/Milieukunde (BIOL); International (English)

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APA (6^th Edition):

Hofman, E. G. (2008). Plasma membrane organization during EGFR signaling: a FRET-based analysis. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/30336

Chicago Manual of Style (16^th Edition):

Hofman, E G. “Plasma membrane organization during EGFR signaling: a FRET-based analysis.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/30336.

MLA Handbook (7^th Edition):

Hofman, E G. “Plasma membrane organization during EGFR signaling: a FRET-based analysis.” 2008. Web. 25 Jan 2020.

Vancouver:

Hofman EG. Plasma membrane organization during EGFR signaling: a FRET-based analysis. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/30336.

Council of Science Editors:

Hofman EG. Plasma membrane organization during EGFR signaling: a FRET-based analysis. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/30336

Florida International University

3. Rehm, Evan. Factors Affecting Current and Future Treeline Locations and Dynamics in the Peruvian Andes.

Degree: PhD, Biology, 2015, Florida International University

URL: https://digitalcommons.fiu.edu/etd/1836 ; 10.25148/etd.FI15032158 ; FI15032158

► The elevational distributions of tropical treelines are thought to be determined by temperature, and are predicted to shift upslope in response to global warming.… (more)

▼ The elevational distributions of tropical treelines are thought to be determined by temperature, and are predicted to shift upslope in response to global warming. In contrast to this hypothesis, global-scale studies have shown that only half of all studied treelines are shifting upslope. Understanding how treelines will respond to climate change has important implications for global biodiversity, especially in the tropics, because tropical treelines generally represent the upper-elevation distribution limit of the hyper-diverse cloudforest ecosystem. In Chapter 1, I introduce the idea that grasslands found above tropical treelines may represent a potential grass ceiling which forest species cannot cross or invade. I use an extensive literature review to outline potential mechanisms which may be acting to stabilize treeline and prevent forest expansion into high-elevation grasslands. In Chapters 2-4, I begin to explore these potential mechanisms through the use of observational and experimental methods. In Chapter 2, I show that there are significant numbers of seedlings occurring just outside of the treeline in the open grasslands and that seed rain is unlikely to limit seedling recruitment above treeline. I also show that microclimates outside of the closed-canopy cloudforest are highly variable and that mean temperatures are likely a poor explanation of tropical treeline elevations. In Chapter 3, I show that juvenile trees maintain freezing resistances similar to adults, but nighttime radiative cooling near the ground in the open grassland results in lower cold temperatures relative to the free atmosphere, exposing seedlings of some species growing above treeline to lethal frost events. In Chapter 4, I use a large-scale seedling transplant experiment to test the effects of mean temperature, absolute low temperature and shade on transplanted seedling survival. I find that increasing mean temperature negatively affects seedling survival of two treeline species while benefiting another. In addition, low temperature extremes and the presence of shade also appear to be important factors affecting seedling survival above tropical treelines. This work demonstrates that mean temperature is a poor predictor of tropical treelines and that temperature extremes, especially low temperatures, and non-climatic variables should be included in predictions of current and future tropical treeline dynamics. Advisors/Committee Members: Ken Feeley, J. Albert Uy, Steven F. Oberbauer, Hong Liu, John Withey.

Subjects/Keywords: Life sciences; biology; Biology; Life Sciences

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APA (6^th Edition):

Rehm, E. (2015). Factors Affecting Current and Future Treeline Locations and Dynamics in the Peruvian Andes. (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/1836 ; 10.25148/etd.FI15032158 ; FI15032158

Chicago Manual of Style (16^th Edition):

Rehm, Evan. “Factors Affecting Current and Future Treeline Locations and Dynamics in the Peruvian Andes.” 2015. Doctoral Dissertation, Florida International University. Accessed January 25, 2020. https://digitalcommons.fiu.edu/etd/1836 ; 10.25148/etd.FI15032158 ; FI15032158.

MLA Handbook (7^th Edition):

Rehm, Evan. “Factors Affecting Current and Future Treeline Locations and Dynamics in the Peruvian Andes.” 2015. Web. 25 Jan 2020.

Vancouver:

Rehm E. Factors Affecting Current and Future Treeline Locations and Dynamics in the Peruvian Andes. [Internet] [Doctoral dissertation]. Florida International University; 2015. [cited 2020 Jan 25]. Available from: https://digitalcommons.fiu.edu/etd/1836 ; 10.25148/etd.FI15032158 ; FI15032158.

Council of Science Editors:

Rehm E. Factors Affecting Current and Future Treeline Locations and Dynamics in the Peruvian Andes. [Doctoral Dissertation]. Florida International University; 2015. Available from: https://digitalcommons.fiu.edu/etd/1836 ; 10.25148/etd.FI15032158 ; FI15032158

Universiteit Utrecht

4. Westra, R.H.V. Learning and teaching ecosystem behaviour in secondary education : Systems thinking and modelling in authentic practices.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/26253

► This thesis describes developmental research, aiming at a useful approach for modern secondary ecology education. The research question is: What are the characteristics of a… (more)

▼ This thesis describes developmental research, aiming at a useful approach for modern secondary ecology education. The research question is: What are the characteristics of a valid, feasible and effective learning and teaching strategy about ecosystem behaviour using modelling and systems thinking in authentic practices? Knowledge from and insight in complexity and dynamics of an ecosystem has societal relevance. Because of lack of such knowledge and insight, secondary students do not have the opportunity to develop ‘ecological literacy’. By making use of systems thinking and modelling, complexity and dynamics can become transparent for students who perform these activities themselves. In the Netherlands there has been a shift from transmission of knowledge towards construction of knowledge, where the teacher gets the role of a coach, more than as the transmitter of knowledge. The cultural historical approach is interested in the teacher’s role as coach as well as ‘mediator’ between students and culture. Here, learning requires a practice that invites students to participate in activities. The authenticity of the practice could provide students with motives for learning. A concept may have different meanings in different practices (or contexts). Students have to adapt this concept when they use it in another practice: re-contextualisation. For education to be effective, students should be willing and enabled to take an active role in the learning activities. The use of authentic practices will not be sufficient. The problem posing approach aims to actively involve students in the learning process. Therefore the concept-context-approach is linked with this approach. After raising modern scientific ideas of the concept ‘ecosystem’, the present situation in secondary education is investigated. Ecology education uses static models. In examination syllabus, schoolbooks and lessons there are hardly any traces of modern ecological views. Ecologists and teachers differ substantially in their views on the concept ‘ecosystem’. After determining learning aims derived from the modern concept ‘ecosystem’, design criteria are formulated for a learning and teaching strategy. Next, a selection is made of three authentic practices. The developed strategy has been tested in classroom to provide an answer to the research question. This strategy appears to be valid, in terms of introducing adequate ecosystem-related practices where dynamics and complexity are important sub-concepts, enabling students to understand ecosystem behaviour. It is feasible, in terms of the characteristics of a realistic, clear and relevant use of practices, with a transparent role for systems thinking; where there is a problem that elicits student’s ideas; and where the strategy is clear to the students. However, in terms of the characteristics of the systems character of an ecosystem, the modelling activities and the plausibility of the sequence of the practices, the strategy is not (completely) feasible. In some aspects the strategy is effective. Most… Advisors/Committee Members: Boersma, Kerst, Waarlo, Arend Jan, Savelsbergh, Elwin.

Subjects/Keywords: Life sciences; Biologie/Milieukunde (BIOL); International (English)

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APA (6^th Edition):

Westra, R. H. V. (2008). Learning and teaching ecosystem behaviour in secondary education : Systems thinking and modelling in authentic practices. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/26253

Chicago Manual of Style (16^th Edition):

Westra, R H V. “Learning and teaching ecosystem behaviour in secondary education : Systems thinking and modelling in authentic practices.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/26253.

MLA Handbook (7^th Edition):

Westra, R H V. “Learning and teaching ecosystem behaviour in secondary education : Systems thinking and modelling in authentic practices.” 2008. Web. 25 Jan 2020.

Vancouver:

Westra RHV. Learning and teaching ecosystem behaviour in secondary education : Systems thinking and modelling in authentic practices. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/26253.

Council of Science Editors:

Westra RHV. Learning and teaching ecosystem behaviour in secondary education : Systems thinking and modelling in authentic practices. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/26253

Universiteit Utrecht

5. Kornet, N.G. Role of chromatin factors in Arabidopsis root stem cell maintenance.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/27635

► Stem cells replenish the cells present in an organism throughout its lifetime and sustain growth. They have unique characteristics: the capability to self-renew and the… (more)

▼ Stem cells replenish the cells present in an organism throughout its lifetime and sustain growth. They have unique characteristics: the capability to self-renew and the potential to differentiate into several cell types. Recently, it has become clear that chromatin factors support these unique features in mammalian stem cells (Chapter 1). The role of chromatin factors in plant stem cell control is just starting to be revealed. In this thesis, we describe investigations on the roles of chromatin factors in Arabidopsis root stem cell maintenance. We performed a genome-wide survey of chromatin factor to identify which classes of chromatin factors could be genetically linked to stem cell action. In addition, we investigated the relationship of these chromatin factors with existing stem cell regulatory pathways. We find that several classes of chromatin factors (histone acetyltransferase GCN5, the CAF-1 complex, histone deacetylases (HDACs), PRC2 Polycomb proteins) regulate root stem cell niche maintenance. We show that GCN5 affects root stem cell niche maintenance mainly through expression regulation of stem cell transcription factor PLETHORA (PLT) (Chapter 2). The GCN5 associated factor ADA2b is not involved in stem cell niche maintenance, but mediates proliferation of the transit amplifying cells directly. Furthermore, we show that the chromatin assembly factor 1 (CAF-1) complex and RETINOBLASTOMA RELATED (RBR) repress synergistically root stem cell proliferation through regulation of stem cell niche transcription factors and promote correct differentiation (Chapter 3). The CAF-1 complex acts in the SHORTROOT (SHR)/ SCARECROW (SCR) pathway. We provide evidence that the CAF-1 complex and RBR may ensure propagation of repressive epigenetic marks at overlapping target genes together with HDACs and in this way play an important role in epigenetic stem cell control. In addition, we show that PRC2 Polycomb proteins SWINGER (SWN) and CURLY LEAF (CLF) are redundantly required to maintain the stem cells and expression of the root stem cell transcription factor SCR (Chapter 4). Our data reveal a novel function for RBR: it is redundantly required with SWN and CLF to stimulate proliferation or prevent differentiation of the transit amplifying cells. Finally, the results described in this thesis are discussed in light of recent insights into the specialized chromatin state in stem cells (Chapter 5). In conclusion, the research performed in this thesis reveals that several classes of chromatin factors are required for root stem cell maintenance and play a role in both parallel pathways known to define the root stem cells. In mammals, pluripotent stem cell transcription factors probably regulate chromatin factors to obtain a special chromatin state in ES cells. Similarly, in Arabidopsis, stem cell transcription factors are sufficient to induce root or shoot stem cells and it is an interesting possibility that they regulate chromatin factors, whereas it is already evident that chromatin factors regulate stem cell transcription… Advisors/Committee Members: Scheres, Bernardus.

Subjects/Keywords: Life sciences; Biologie/Milieukunde (BIOL); International (English)

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APA (6^th Edition):

Kornet, N. G. (2008). Role of chromatin factors in Arabidopsis root stem cell maintenance. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/27635

Chicago Manual of Style (16^th Edition):

Kornet, N G. “Role of chromatin factors in Arabidopsis root stem cell maintenance.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/27635.

MLA Handbook (7^th Edition):

Kornet, N G. “Role of chromatin factors in Arabidopsis root stem cell maintenance.” 2008. Web. 25 Jan 2020.

Vancouver:

Kornet NG. Role of chromatin factors in Arabidopsis root stem cell maintenance. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/27635.

Council of Science Editors:

Kornet NG. Role of chromatin factors in Arabidopsis root stem cell maintenance. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/27635

Universiteit Utrecht

6. Koornneef, A. Cross-talk in plant defense signaling: Antagonism between salicylate and jasmonate pathways in Arabidopsis.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/27163

► Plants are equipped with an array of defense mechanisms to protect themselves against herbivorous insects and microbial pathogens. Some are pre-existing while others are only… (more)

▼ Plants are equipped with an array of defense mechanisms to protect themselves against herbivorous insects and microbial pathogens. Some are pre-existing while others are only activated upon insect attack or pathogen invasion. However, induced defense responses entail fitness costs. Therefore, plants possess elaborate regulatory mechanisms that efficiently coordinate activation of attacker-specific defenses. A major focus in plant defense signaling research is to uncover key mechanisms by which plants tailor their responses to different attackers, and to investigate how plants cope with simultaneous interactions with multiple aggressors. The phytohormones salicylic acid (SA) and jasmonic acid (JA) play a major role in the regulation of induced defense mechanisms against biotrophic pathogens and insect herbivores, respectively. Cross-talk between SA and JA defense signaling pathways is thought to allow fine-tuning of the defense response to the attacker encountered. In Arabidopsis, pharmacological experiments revealed that SA exerts a strong antagonistic effect on JA-responsive genes, such as PLANT DEFENSIN1.2 (PDF1.2), indicating that the SA pathway can be prioritized over the JA pathway. This antagonistic effect of SA on JA signaling is a robust phenomenon: It is conserved among Arabidopsis accessions, and JA-responsive gene expression is readily suppressed by SA for several days, even when triggered by very low doses of SA. Time interval studies revealed that SA has a window of opportunity to suppress MeJA-responsive gene expression, and that this time interval correlates with the SA-induced redox change in the plant tissue. Thus, redox modulation is likely to play a central role in the regulatory mechanism underlying SA/JA cross-talk. Analysis of the Arabidopsis mutant npr1, impaired in SA signal transduction, revealed that the antagonistic effect of SA on JA signaling requires the regulatory protein NPR1. Nuclear localization of NPR1, which is essential for SA-mediated defense gene expression, is not required for suppression of JA-responsive genes, indicating that cross-talk between SA and JA is modulated through a function of NPR1 in the cytosol. TGA transcription factors are similarly required for both SA-dependent gene expression and SA-mediated suppression of JA signaling. However, TGA factors are unlikely to exert their effect through direct interaction with the promoter of JA-responsive genes such as PDF1.2, as removal of the TGA binding site in this promoter did not affect cross-talk. Promoter analysis of SA-suppressed, MeJA-inducible cross-talk genes revealed enrichment of the I box and the GCC box motif. Site-directed mutagenesis of the I box motif in the PDF1.2 promoter did not affect suppression of this gene by SA, indicating that the I box is not essential in mediating cross-talk. The role of the GCC box in cross-talk regulation should be further elucidated. This motif is required for MeJA-responsiveness in the PDF1.2 promoter, and thus provides an attractive target for SA-mediated suppression.… Advisors/Committee Members: Pieterse, Corné, van Loon, L.C..

Subjects/Keywords: Plant biology (Botany); Life sciences; Biologie/Milieukunde (BIOL)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Koornneef, A. (2008). Cross-talk in plant defense signaling: Antagonism between salicylate and jasmonate pathways in Arabidopsis. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/27163

Chicago Manual of Style (16^th Edition):

Koornneef, A. “Cross-talk in plant defense signaling: Antagonism between salicylate and jasmonate pathways in Arabidopsis.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/27163.

MLA Handbook (7^th Edition):

Koornneef, A. “Cross-talk in plant defense signaling: Antagonism between salicylate and jasmonate pathways in Arabidopsis.” 2008. Web. 25 Jan 2020.

Vancouver:

Koornneef A. Cross-talk in plant defense signaling: Antagonism between salicylate and jasmonate pathways in Arabidopsis. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/27163.

Council of Science Editors:

Koornneef A. Cross-talk in plant defense signaling: Antagonism between salicylate and jasmonate pathways in Arabidopsis. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/27163

Universiteit Utrecht

7. Vermeulen, P.J. Plastic responses in the competition for light among genotypes of a stoloniferous species.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/26978

► The ability to adjust the phenotype (e.g. plasticity) is thought to be beneficial for the performance of a plant, because it prevents overinvestment in support… (more)

▼ The ability to adjust the phenotype (e.g. plasticity) is thought to be beneficial for the performance of a plant, because it prevents overinvestment in support structures when plant density is low, and allows a plant to position its leaves high in the canopy when density is high. However, as the plastic responses differ within species, exclusion of less responsive genotypes from dense vegetation is likely to occur. A 5 year old competition experiment between 10 genotypes of the stoloniferous plant Potentilla reptans was used to study the consequences of the genotypic differences in plasticity, and the interplay between these genotypes, in order to gain more insights in the mechanisms behind the competition for light Within the competition experiment, leaves that were positioned higher in the canopy had higher light interception and higher photosynthetic rates per unit invested biomass than lower places leaves. Placing laminas at the top of the canopy is thus of great importance to the performance of a genotype. Using artificially created light gradients, it was also shown that the speed of height increase determines which genotypes can place their leaves at the top. Height increase of the canopy differed between mono-genotypic stands, and thus depends on the genotypes that form the canopy. A canopy model of the competition experiment showed that the dominant genotype had rather shade tolerant photosynthetic characteristics, which allowed it to have positive carbon gain at the bottom of the vegetation. Therefore, low leaf turnover may have contributed to the abundance of this genotype. Coexistence could than occur because other genotypes have a higher carbon gain in the top layers of the canopy. A game theoretical analysis of the canopy model showed that for all genotypes the total lamina area that would maximize the photosynthetic rate of the genotype itself was higher than total the lamina area that would maximize the photosynthetic rate of the vegetation as whole. Selection would thus favor the former strategy, leading to lower biomass production of the whole canopy. Finally, the analyses indicate that a decrease of the total lamina area of the dominant genotypes would increase its own photosynthetic rate, but that it would increase the performance of other genotypes more. Yet although a mutation in the lamina area of the dominant genotype would not increase its performance, the vegetation is not evolutionarily stable, as a mutant in the lamina area of the other genotypes would lead to better performance of these mutants. Overall the results suggest that the performance of an individual is not only determined by its own characteristics, but also by that of its competitors. In addition to the ability to reach the top of canopy, relatively shade tolerant characteristics may be selected for if plant density is high. The findings also demonstrate that is important to know at what level of organization selection operates. The understanding of competitive exclusion would greatly benefit if plastic response were studied… Advisors/Committee Members: Werger, M, During, Heinjo.

Subjects/Keywords: Plant biology (Botany); Life sciences; Biologie/Milieukunde (BIOL)

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APA (6^th Edition):

Vermeulen, P. J. (2008). Plastic responses in the competition for light among genotypes of a stoloniferous species. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/26978

Chicago Manual of Style (16^th Edition):

Vermeulen, P J. “Plastic responses in the competition for light among genotypes of a stoloniferous species.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/26978.

MLA Handbook (7^th Edition):

Vermeulen, P J. “Plastic responses in the competition for light among genotypes of a stoloniferous species.” 2008. Web. 25 Jan 2020.

Vancouver:

Vermeulen PJ. Plastic responses in the competition for light among genotypes of a stoloniferous species. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/26978.

Council of Science Editors:

Vermeulen PJ. Plastic responses in the competition for light among genotypes of a stoloniferous species. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/26978

Universiteit Utrecht

8. Heydarian, Z. Transcriptome and kinome analysis during ethylene-induced growth in Rumex palustris.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/27674

► Complete submergence is one of the most severe abiotic stresses worldwide, both in natural and man-made ecosystems. A limited number of species have evolved mechanisms… (more)

▼ Complete submergence is one of the most severe abiotic stresses worldwide, both in natural and man-made ecosystems. A limited number of species have evolved mechanisms to deal with these conditions. Rumex palustris, a model for studying plant responses to flooding, manages to outgrow the water, and thus restore contact with the atmosphere, through upward leaf growth (hyponasty) followed by strongly enhanced petiole elongation. These responses are initiated by the gaseous plant hormone ethylene, which accumulates inside plants due to physical entrapment and mimics largely this morphological submergence response. The complexity of ethylene-driven growth in R. palustris upon submergence suggests the involvement of many genes under tight regulation. A comprehensive understanding of the changes in the expression of genes responsive to ethylene is crucial to elucidate the signal transduction networks that lead to hyponastic growth and under-water elongation in this plant. By applying cDNA subtractive hybridisation and cDNA-AFLP (RNA fingerprinting) we identified 119 differentially expressed genes during ethylene-induced hyponastic growth and petiole elongation. From those, 21 have very good homology with Arabidopsis genes. The expression kinetics of these genes were monitored in response to ethylene and submergence using Real Time RTPCR and the putative involvement of candidate genes in (differential) growth during ethylene and submergence treatment was confirmed. Furthermore, we applied whole genome profiling by hybridising mRNA from R. palustris to a CATMA spotted array containing Arabidopsis gene specific tags. This resulted in more than 5000 genes that were differentially regulated in one or both traits. Additionally, the presence of transcription factor binding motifs in the promoters of the differentially expressed genes were examined and demonstrated an over-representation of Abscisic acid responsive elements. Aanalysing the function of putative R. palustris orthologous genes in Arabidopsis during ethylene-induced hyponastic growth revealed that a subset of these genes is likely to control the initial angle of the petiole since, mutations in these genes resulted in constitutive elevated initial petiole angles in Arabidopsis mutant plants compared to the wild type Columbia-0. The others have an effect on either the angle of the petiole or leaf blade angle or both. Mutant analysis revealed a role for PSI-H subunit of photosystem I, glyceraldehyde-3-phosphate dehydrogenase and glutamate synthase in hyponastic growth of Arabidopsis. Furthermore, the effect of auxin response factor ARF8 on hyponastic growth was demonstrated. Reversible protein phosphorylation is a fundamental strategy used by eukaryotes to regulate basic cellular function. Upon a variety of stimuli, kinases phosphorylate downstream kinases usually until the target transcription factor is phosphorylated. Moreover, for many metabolic processes reversible phosphorylation of proteins is a key regulatory mechanism. We examined changes in the kinome profile of… Advisors/Committee Members: Voesenek, Laurentius, Peeters, Ton.

Subjects/Keywords: Plant biology (Botany); Life sciences; Biologie/Milieukunde (BIOL)

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APA (6^th Edition):

Heydarian, Z. (2008). Transcriptome and kinome analysis during ethylene-induced growth in Rumex palustris. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/27674

Chicago Manual of Style (16^th Edition):

Heydarian, Z. “Transcriptome and kinome analysis during ethylene-induced growth in Rumex palustris.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/27674.

MLA Handbook (7^th Edition):

Heydarian, Z. “Transcriptome and kinome analysis during ethylene-induced growth in Rumex palustris.” 2008. Web. 25 Jan 2020.

Vancouver:

Heydarian Z. Transcriptome and kinome analysis during ethylene-induced growth in Rumex palustris. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/27674.

Council of Science Editors:

Heydarian Z. Transcriptome and kinome analysis during ethylene-induced growth in Rumex palustris. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/27674

Universiteit Utrecht

9. van Kuijk, M. Forest regeneration and restoration in Vietnam.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/27799

► It is generally thought that asymmetric competition for light and asymmetric growth, determines the course of succession in a regenerating secondary tropical forest, but it… (more)

▼ It is generally thought that asymmetric competition for light and asymmetric growth, determines the course of succession in a regenerating secondary tropical forest, but it is hardly ever quantified. In this study a shrub and three woody tree species were monitored in time during the first five years of succession. Light competition and light-use were related to the success of species. It appeared that in a slow growing vegetation the importance of asymmetric competition for light and growth can be much less than often assumed. The species composition that was present from the very early beginning of succession remained for at least the first five years. Nitrogen-use efficiency may partly determine a plant’s success during forest regeneration. Whole canopy nitrogen-use efficiency and its underlying traits were compared among pioneer species over the first five years of succession. Nitrogen-use efficiency was largely determined by leaf life span and resorption and differed twofold among species. It was however not related to growth rates and only partly to species height. Nitrogen-use efficiency was slightly different within a species between successional stands that differed in height, leaf area index and resource availability, but an increase in competitive pressure did not result in major changes in the use of nitrogen. Forest regeneration is often slow or stagnates due to the excessive growth of non-woody plants and shrubs. One often used method to accelerate succession, called liberation, is opening up the vegetation canopy around young target trees which increases their growth. A 3D-model is used which enables us to examine how stature, crown structure and physiological traits of target trees and characteristics of the surrounding vegetation together determine the growth of trees. The model was applied to a liberation experiment that was conducted on pioneer species in a young secondary forest. Species responded differently to the treatment depending on their height, crown structure and their light demands. The responses were also dependent on the height and density of the vegetation and the gap radius from which it was removed. Another method to enhance regeneration is by planting tree species in the existing vegetation. Lines are cut and overstory thinning is applied to increase light levels. The line width and the degree of overstory thinning affect growth of target species differently depending on the characteristics of the surrounding vegetation and the light demands of the target trees themselves. The same model approach was used to test for the effects of line width, the degree of overstory thinning, planting trees in different successional stands of the same forest and planting trees in a forest stand with different light levels than the stand in which they were originally grown. It was shown that the optimal stand for each species, the optimal line width in a stand and the optimal degree of overstory thinning, can be predicted. With the model approach presented here it is possible to simulate different… Advisors/Committee Members: Werger, M, Anten, Niels.

Subjects/Keywords: Plant biology (Botany); Life sciences; Biologie/Milieukunde (BIOL)

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APA (6^th Edition):

van Kuijk, M. (2008). Forest regeneration and restoration in Vietnam. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/27799

Chicago Manual of Style (16^th Edition):

van Kuijk, M. “Forest regeneration and restoration in Vietnam.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/27799.

MLA Handbook (7^th Edition):

van Kuijk, M. “Forest regeneration and restoration in Vietnam.” 2008. Web. 25 Jan 2020.

Vancouver:

van Kuijk M. Forest regeneration and restoration in Vietnam. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/27799.

Council of Science Editors:

van Kuijk M. Forest regeneration and restoration in Vietnam. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/27799

Universiteit Utrecht

10. Huibers, R.P. Genetic analysis of susceptibility of Arabidopsis to downy mildew infection.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/29706

► The mechanisms by which host plants contribute to their colonization by hemibiotrophic and biotrophic fungal and oomycete pathogens is a poorly understand aspect of molecular… (more)

▼ The mechanisms by which host plants contribute to their colonization by hemibiotrophic and biotrophic fungal and oomycete pathogens is a poorly understand aspect of molecular plant pathology. The aim of this study was to identify Arabidopsis genes controlling cellular processes that influence susceptibility to the biotrophic oomycete Hyaloperonospora parasitica. Chapter 2 describes a forward genetic approach to identify Arabidopsis mutants that show loss of compatibility to H. parasitica. Six of these so called downy mildew resistant (dmr) mutants were characterized in more detail. dmr3 and dmr4 show resistance to a broad range of pathogens whereas dmr1, dmr2, dmr5 and dmr6 show a type of resistance more specific to H. parasitica. Chapter 3 shows that constitutive high expression of defense related genes in the dmr5 mutant contributes to resistance to H. parasitica. Surprisingly, it was found that dmr5 mutants were mutated in RPM1, a resistance gene of the NBS-LRR class that confers resistance to Pseudomonas syringae bacteria carrying the avrB or avrRPM1 genes. RPM1 function in dmr5 mutants is largely, but not completely, lost. Genetic data indicate that dmr5 encodes a mild autoactive form of RPM1. Further studies on the mutant RPM1 protein could provide more insight to R protein function. Chapter 4 describes the map based cloning of DMR6 that encodes a putative 2OG-Fe(II) oxygenase. DMR6 is strongly induced in infected host cells during both compatible and incompatible interactions with H. parasitica. DMR6 is also highly induced after application of BTH and is constitutively high expressed in broad range disease resistance mutants. Absence of a functional DMR6 gene results in resistance to H. parasitica, that is accompanied with high expression of several defense-related genes including DMR6 itself. Together, the obtained data indicate that DMR6 is a negative regulator of defense. Chapter 5 describes the identification of a group of Arabidopsis genes that is induced during compatible interactions but not during incompatible interactions. Most of these so called compatible-specific genes (CS-genes) are also induced during various abiotic stress conditions such as cold, dehydration, high salt and high light, but also in response to ABA. The activation of CS-genes can either reflect a physiological response on pathogen infection or be triggered by pathogen effector proteins. As the CS-genes are not induced as part of a defense response they provide a first glimpse into non-defense related processes during compatible interactions. Advisors/Committee Members: Weisbeek, P.J., van den Ackerveken, Guido.

Subjects/Keywords: Plant biology (Botany); Life sciences; Biologie/Milieukunde (BIOL); International (English)

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APA (6^th Edition):

Huibers, R. P. (2008). Genetic analysis of susceptibility of Arabidopsis to downy mildew infection. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/29706

Chicago Manual of Style (16^th Edition):

Huibers, R P. “Genetic analysis of susceptibility of Arabidopsis to downy mildew infection.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/29706.

MLA Handbook (7^th Edition):

Huibers, R P. “Genetic analysis of susceptibility of Arabidopsis to downy mildew infection.” 2008. Web. 25 Jan 2020.

Vancouver:

Huibers RP. Genetic analysis of susceptibility of Arabidopsis to downy mildew infection. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/29706.

Council of Science Editors:

Huibers RP. Genetic analysis of susceptibility of Arabidopsis to downy mildew infection. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/29706

Universiteit Utrecht

11. Tata, M.H.L. Mycorrhizae on dipterocarps in rubber agroforests (RAF) in Sumatra.

Degree: 2008, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/31556

► In tropical lowland forests, which dominated by trees of Dipterocarpaceae family, trees and fungi are strongly linked, through ectomycorrhiza (EcM) formation. However, as part of… (more)

▼ In tropical lowland forests, which dominated by trees of Dipterocarpaceae family, trees and fungi are strongly linked, through ectomycorrhiza (EcM) formation. However, as part of rapid deforestation and forest transformation, mixed dipterocarp forests have been replaced by other vegetation and land use types. Considerable areas of the lowland tropical forest in Sumatra have been turned into Rubber Agroforests (RAF). RAF is a land use type in which rubber trees are planted, while allowing spontaneous establishment of forest tree species. The method presented in this thesis includes four main elements. Firstly, vegetation analysis in seven types of land use. Secondly, study in a nursery using dipterocarps species as bait plant to assess the effect of land use change on EcM inoculum and to analyze the effect of soil heating and drying on EcM propagules. Thirdly, an in-situ experiment in RAF with different history was done to assess whether fungal inoculant increased the survival, growth, nutrient uptake and EcM formation on Shorea selanica and Shorea lamellata. Finally, molecular techniques of PCR and sequencing have been applied to identify EcM fungal symbionts on dipterocarps. The study has been conducted in Jambi province, Sumatra, Indonesia. The ex-situ experiment has been conducted in the nursery of the Forest Research and Development Agency, Bogor, Indonesia. Identification of EcM fungi was done in the Fungal Biodiversity Centre (CBS), Utrecht, the Netherlands. The result of vegetation analysis showed that human activity in RAF management has strong effects on species richness. Very few trees dependent on EcM fungi were encountered in RAF. Our study in the nursery shows small differences in EcM colonization between soils derived from a wide range of land use types. It may indicate that survival of spores and colonization potential are not sensitive to the history of the site. Rapid recolonization of the heated soil in the experiment may imply sufficient availability of EcM fungi for dipterocarps under field conditions, where fire as a tool for land clearing is commonly used in Sumatra. The in-situ experiment in the RAF showed that lack of trees that are dependent on EcM fungi in RAF and in non-forested land does not necessarily imply the absence of EcM inoculum belowground in the ecosystem. EcM inoculum persists in the soil after forest was changed to RAF. Molecular identification of EcM fungi showed that Tomentella was the prevalent genus of EcM fungi colonizing dipterocarp seedlings in nursery stage and in the in-situ test 1 year after the seedlings have been planted in the field. None of the fungi was identified as Scleroderma columnare, which was inoculated to S. lamellata and S. selanica seedlings in the nursery stage. This indicated that indigenous EcM fungi dominated on the dipterocarp seedlings planted in the field, regardless of nursery inoculation. The implementation of this study is potentially of great value for reforestation efforts with dipterocarps in RAF in collaboration with rubber farmers. This… Advisors/Committee Members: Werger, M, de Hoog, G.S., van Noordwijk, M., Summerbell, R.C..

Subjects/Keywords: Plant biology (Botany); Life sciences; Biologie/Milieukunde (BIOL)

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APA (6^th Edition):

Tata, M. H. L. (2008). Mycorrhizae on dipterocarps in rubber agroforests (RAF) in Sumatra. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/31556

Chicago Manual of Style (16^th Edition):

Tata, M H L. “Mycorrhizae on dipterocarps in rubber agroforests (RAF) in Sumatra.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/31556.

MLA Handbook (7^th Edition):

Tata, M H L. “Mycorrhizae on dipterocarps in rubber agroforests (RAF) in Sumatra.” 2008. Web. 25 Jan 2020.

Vancouver:

Tata MHL. Mycorrhizae on dipterocarps in rubber agroforests (RAF) in Sumatra. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2020 Jan 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/31556.

Council of Science Editors:

Tata MHL. Mycorrhizae on dipterocarps in rubber agroforests (RAF) in Sumatra. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/31556

Michigan Technological University

12. Paladino, Louis Paul-Anthony. Screening, optimization and extraction of polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium.

Degree: MS, Department of Biological Sciences, 2009, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/497

► Bioplastics are polymers (such as polyesters) produced from bacterial fermentations that are biodegradable and nonhazardous. They are produced by a wide variety of bacteria… (more)

▼ Bioplastics are polymers (such as polyesters) produced from bacterial fermentations that are biodegradable and nonhazardous. They are produced by a wide variety of bacteria and are made only when stress conditions allow, such as when nutrient levels are low, more specifically levels of nitrogen and oxygen. These stress conditions cause certain bacteria to build up excess carbon deposits as energy reserves in the form of polyhydroxyalkanoates (PHAs). PHAs can be extracted and formed into actual plastic with the same strength of conventional, synthetic-based plastics without the need to rely on foreign petroleum. The overall goal of this project was to select for a bacteria that could grow on sugars found in the lignocellulosic biomass, and get the bacteria to produce PHAs and peptidoglycan. Once this was accomplished the goal was to extract PHAs and peptidoglycan in order to make a stronger more rigid plastic, by combing them into a co-polymer. The individual goals of this project were to: (1) Select and screen bacteria that are capable of producing PHAs by utilizing the carbon/energy sources found in lignocellulosic biomass; (2) Maximize the utilization of those sugars present in woody biomass in order to produce optimal levels of PHAs. (3) Use room temperature ionic liquids (RTILs) in order to separate the cell membrane and peptidoglycan, allowing for better extraction of PHAs and more intact peptidoglycan. B. megaterium a Gram-positive PHA-producing bacterium was selected for study in this project. It was grown on a variety of different substrates in order to maximize both its growth and production of PHAs. The optimal conditions were found to be 30°C, pH 6.0 and sugar concentration of either 30g/L glucose or xylose. After optimal growth was obtained, both RTILs and enzymatic treatments were used to break the cell wall, in order to extract the PHAs, and peptidoglycan. PHAs and peptidoglycan were successfully extracted from the cell, and will be used in the future to create a new stronger co-polymer. Peptidoglycan recovery yield was 16% of the cells’ dry weight. Advisors/Committee Members: Susan T. Bagley.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Paladino, L. P. (2009). Screening, optimization and extraction of polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/497

Chicago Manual of Style (16^th Edition):

Paladino, Louis Paul-Anthony. “Screening, optimization and extraction of polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium.” 2009. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/497.

MLA Handbook (7^th Edition):

Paladino, Louis Paul-Anthony. “Screening, optimization and extraction of polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium.” 2009. Web. 25 Jan 2020.

Vancouver:

Paladino LP. Screening, optimization and extraction of polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium. [Internet] [Masters thesis]. Michigan Technological University; 2009. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/497.

Council of Science Editors:

Paladino LP. Screening, optimization and extraction of polyhydroxyalkanoates and peptidoglycan from Bacillus megaterium. [Masters Thesis]. Michigan Technological University; 2009. Available from: http://digitalcommons.mtu.edu/etds/497

Michigan Technological University

13. Ebel, Jonathan D. Biofilm responses to salmon carcass analog addition in central Idaho streams.

Degree: MS, Department of Biological Sciences, 2012, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/186

► Pacific salmon populations have declined due to human activity in the Pacific Northwest, resulting in decreased delivery of marine-derived nutrients to streams. Managers use… (more)

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Ebel, J. D. (2012). Biofilm responses to salmon carcass analog addition in central Idaho streams. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/186

Chicago Manual of Style (16^th Edition):

Ebel, Jonathan D. “Biofilm responses to salmon carcass analog addition in central Idaho streams.” 2012. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/186.

MLA Handbook (7^th Edition):

Ebel, Jonathan D. “Biofilm responses to salmon carcass analog addition in central Idaho streams.” 2012. Web. 25 Jan 2020.

Vancouver:

Ebel JD. Biofilm responses to salmon carcass analog addition in central Idaho streams. [Internet] [Masters thesis]. Michigan Technological University; 2012. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/186.

Council of Science Editors:

Ebel JD. Biofilm responses to salmon carcass analog addition in central Idaho streams. [Masters Thesis]. Michigan Technological University; 2012. Available from: http://digitalcommons.mtu.edu/etds/186

Michigan Technological University

14. Lucas, Angela K. Effects of pediatric adiposity on heart rate variability.

Degree: MS, Department of Biological Sciences, 2009, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/189

► The relationship between obesity and heart rate variability (HRV) has been studied in adults and adolescents, but is not determined in young pediatrics. The… (more)

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Lucas, A. K. (2009). Effects of pediatric adiposity on heart rate variability. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/189

Chicago Manual of Style (16^th Edition):

Lucas, Angela K. “Effects of pediatric adiposity on heart rate variability.” 2009. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/189.

MLA Handbook (7^th Edition):

Lucas, Angela K. “Effects of pediatric adiposity on heart rate variability.” 2009. Web. 25 Jan 2020.

Vancouver:

Lucas AK. Effects of pediatric adiposity on heart rate variability. [Internet] [Masters thesis]. Michigan Technological University; 2009. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/189.

Council of Science Editors:

Lucas AK. Effects of pediatric adiposity on heart rate variability. [Masters Thesis]. Michigan Technological University; 2009. Available from: http://digitalcommons.mtu.edu/etds/189

Michigan Technological University

15. Dhadi, Surendar R. Functional analysis of rice bidirectional promoters.

Degree: PhD, Department of Biological Sciences, 2012, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/191

► Bidirectional promoters regulate adjacent genes organized in a divergent fashion (head to head orientation). Several Reports pertaining to bidirectional promoters on a genomic scale… (more)

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Dhadi, S. R. (2012). Functional analysis of rice bidirectional promoters. (Doctoral Dissertation). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/191

Chicago Manual of Style (16^th Edition):

Dhadi, Surendar R. “Functional analysis of rice bidirectional promoters.” 2012. Doctoral Dissertation, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/191.

MLA Handbook (7^th Edition):

Dhadi, Surendar R. “Functional analysis of rice bidirectional promoters.” 2012. Web. 25 Jan 2020.

Vancouver:

Dhadi SR. Functional analysis of rice bidirectional promoters. [Internet] [Doctoral dissertation]. Michigan Technological University; 2012. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/191.

Council of Science Editors:

Dhadi SR. Functional analysis of rice bidirectional promoters. [Doctoral Dissertation]. Michigan Technological University; 2012. Available from: http://digitalcommons.mtu.edu/etds/191

Michigan Technological University

16. Groves, Stephanie Lee. Optimization of ethanol production by yeasts from lignocellulosic feedstocks.

Degree: MS, Department of Biological Sciences, 2009, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/195

► Ethanol from lignocellulosic feedstocks is not currently competitive with corn-based ethanol in terms of yields and commercial feasibility. Through optimization of the pretreatment and… (more)

▼ Ethanol from lignocellulosic feedstocks is not currently competitive with corn-based ethanol in terms of yields and commercial feasibility. Through optimization of the pretreatment and fermentation steps this could change. The overall goal of this study was to evaluate, characterize, and optimize ethanol production from lignocellulosic feedstocks by the yeasts Saccharomyces cerevisiae (strain Ethanol Red, ER) and Pichia stipitis CBS 6054. Through a series of fermentations and growth studies, P. stipitis CBS 6054 and S. cerevisiae (ER) were evaluated on their ability to produce ethanol from both single substrate (xylose and glucose) and mixed substrate (five sugars present in hemicellulose) fermentations. The yeasts were also evaluated on their ability to produce ethanol from dilute acid pretreated hydrolysate and enzymatic hydrolysate. Hardwood (aspen), softwood (balsam), and herbaceous (switchgrass) hydrolysates were also tested to determine the effect of the source of the feedstock. P. stipitis produced ethanol from 66-98% of the theoretical yield throughout the fermentation studies completed over the course of this work. S. cerevisiae (ER) was determined to not be ideal for dilute acid pretreated lignocellulose because it was not able to utilize all the sugars found in hemicellulose. S. cerevisiae (ER) was instead used to optimize enzymatic pretreated lignocellulose that contained only glucose monomers. It was able to produce ethanol from enzymatically pretreated hydrolysate but the sugar level was so low (>3 g/L) that it would not be commercially feasible. Two lignocellulosic degradation products, furfural and acetic acid, were evaluated for whether or not they had an inhibitory effect on biomass production, substrate utilization, and ethanol production by P. stipitis and S. cerevisiae (ER). It was determined that inhibition is directly related to the concentration of the inhibitor and the organism. The final phase for this thesis focused on adapting P. stipitis CBS 6054 to toxic compounds present in dilute acid pretreated hydrolysate through directed evolution. Cultures were transferred to increasing concentrations of dilute acid pretreated hydrolysate in the fermentation media. The adapted strains’ fermentation capabilities were tested against the unadapted parent strain at each hydrolysate concentration. The fermentation capabilities of the adapted strain were significantly improved over the unadapted parentstrain. On media containing 60% hydrolysate the adapted strain yielded 0.30 g_ethanol/g_sugar ± 0.033 (g/g) and the unadapted parent strain yielded 0.11 g/g ±0.028. The culture has been successfully adapted to growth on media containing 65%, 70%, 75%, and 80% hydrolysate but with below optimal ethanol yields (0.14-0.19 g/g). Cell recycle could be a viable option for improving ethanol yields in these cases. A study was conducted to determine the optimal media for production of ethanol from xylose and mixed substrate… Advisors/Committee Members: Susan T Bagley.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Groves, S. L. (2009). Optimization of ethanol production by yeasts from lignocellulosic feedstocks. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/195

Chicago Manual of Style (16^th Edition):

Groves, Stephanie Lee. “Optimization of ethanol production by yeasts from lignocellulosic feedstocks.” 2009. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/195.

MLA Handbook (7^th Edition):

Groves, Stephanie Lee. “Optimization of ethanol production by yeasts from lignocellulosic feedstocks.” 2009. Web. 25 Jan 2020.

Vancouver:

Groves SL. Optimization of ethanol production by yeasts from lignocellulosic feedstocks. [Internet] [Masters thesis]. Michigan Technological University; 2009. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/195.

Council of Science Editors:

Groves SL. Optimization of ethanol production by yeasts from lignocellulosic feedstocks. [Masters Thesis]. Michigan Technological University; 2009. Available from: http://digitalcommons.mtu.edu/etds/195

Michigan Technological University

17. Haak, Danielle M. Impact of copper mine tailings (stamp sand) on survival and development of aquatic organisms near Gay, Michigan.

Degree: MS, Department of Biological Sciences, 2011, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/192

► Heavy metal-rich copper mine tailings, called stamp sands, were dumped by mining companies directly into streams and along the Lake Superior shoreline, degrading Keweenaw… (more)

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Haak, D. M. (2011). Impact of copper mine tailings (stamp sand) on survival and development of aquatic organisms near Gay, Michigan. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/192

Chicago Manual of Style (16^th Edition):

Haak, Danielle M. “Impact of copper mine tailings (stamp sand) on survival and development of aquatic organisms near Gay, Michigan.” 2011. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/192.

MLA Handbook (7^th Edition):

Haak, Danielle M. “Impact of copper mine tailings (stamp sand) on survival and development of aquatic organisms near Gay, Michigan.” 2011. Web. 25 Jan 2020.

Vancouver:

Haak DM. Impact of copper mine tailings (stamp sand) on survival and development of aquatic organisms near Gay, Michigan. [Internet] [Masters thesis]. Michigan Technological University; 2011. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/192.

Council of Science Editors:

Haak DM. Impact of copper mine tailings (stamp sand) on survival and development of aquatic organisms near Gay, Michigan. [Masters Thesis]. Michigan Technological University; 2011. Available from: http://digitalcommons.mtu.edu/etds/192

Michigan Technological University

18. Doskey, Claire M. Uptake and fate of hexahydro-1,3,5-trinitro-1,3,5-triazine by Chrysopogon zizanioides.

Degree: MS, Department of Biological Sciences, 2012, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/198

► Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a nitramine compound that has been used heavily by the military as an explosive. Manufacturing, use, and disposal of RDX have… (more)

▼ Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a nitramine compound that has been used heavily by the military as an explosive. Manufacturing, use, and disposal of RDX have led to several contamination sites across the United States. RDX is both persistent in the environment and a threat to human health, making its remediation vital. The use of plants to extract RDX from the soil and metabolize it once it is in the plant tissue, is being considered as a possible solution. In the present study, the tropical grass Chrysopogon zizanioides was grown hydroponically in the presence RDX at 3 different concentration levels: 0.3, 1.1, and 2.26 ppm. The uptake of RDX was quantified by high performance liquid chromatography (HPLC) analysis of media samples taken every 6 hr during the first 24 hr and then daily over a 30-day experimental period. A rapid decrease in RDX concentration in the media of both controls and plant treatments was seen within the first 18 hours of the experiment with the greatest loss in RDX over time occurring within the first 6 hours of exposure. The loss was similar in both controls and plant exposures and possibly attributed to rapid uptake by the containers. A plant from one treatment at each of the three concentrations was harvested at Day 10, 20 and 30 throughout the experiment and extracted to determine the localization of RDX within the tissue and potentially identify any metabolites on the basis of differing retention times. Of the treatments containing 0.3, 1.1, and 2.26 ppm RDX, 13.1%, 18.3%, and 24.2% respectively, was quantified in vetiver extracts, with the majority of the RDX being localized to the roots. All plants not yet harvested were harvested on Day 30 of the experiment. A total of three plants exposed to each concentration level as well as the control, were extracted and analyzed with HPLC to determine amount of RDX taken up, localization of RDX within the plant tissue, and potentially identify any metabolites. Phytotoxicity of RDX to vetiver was also monitored. While a loss in biomass was observed in plants exposed to all the different concentrations of RDX, control plants grown in media not exposed to RDX showed the greatest biomass loss of all the treatments. There was also little variation in chlorophyll content between the different concentration treatments with RDX. This preliminary greenhouse study of RDX uptake 10 by Chrysopogon zizanioides will help indicate the potential ability of vetiver to serve as a plant system in the phytoremediation of RDX. Advisors/Committee Members: Rupali Datta, Ramakrishna Wusirika.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Doskey, C. M. (2012). Uptake and fate of hexahydro-1,3,5-trinitro-1,3,5-triazine by Chrysopogon zizanioides. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/198

Chicago Manual of Style (16^th Edition):

Doskey, Claire M. “Uptake and fate of hexahydro-1,3,5-trinitro-1,3,5-triazine by Chrysopogon zizanioides.” 2012. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/198.

MLA Handbook (7^th Edition):

Doskey, Claire M. “Uptake and fate of hexahydro-1,3,5-trinitro-1,3,5-triazine by Chrysopogon zizanioides.” 2012. Web. 25 Jan 2020.

Vancouver:

Doskey CM. Uptake and fate of hexahydro-1,3,5-trinitro-1,3,5-triazine by Chrysopogon zizanioides. [Internet] [Masters thesis]. Michigan Technological University; 2012. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/198.

Council of Science Editors:

Doskey CM. Uptake and fate of hexahydro-1,3,5-trinitro-1,3,5-triazine by Chrysopogon zizanioides. [Masters Thesis]. Michigan Technological University; 2012. Available from: http://digitalcommons.mtu.edu/etds/198

Michigan Technological University

19. Schwartz, Christopher Elmer. Omega-3 fatty acids and the neurovascular responses to mental stress in humans.

Degree: PhD, Department of Biological Sciences, 2011, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/194

► Hypertension is the most prevalent form of cardiovascular disease (CVD) in the world, and is known to increase the risk for developing other diseases.… (more)

▼ Hypertension is the most prevalent form of cardiovascular disease (CVD) in the world, and is known to increase the risk for developing other diseases. Recently, the American Heart Association introduced a new classification of blood pressure, prehypertension (PHT). The criteria for PHT include a systolic of 120-139 mmHg and/or a diastolic blood pressure of 80-89 mmHg. It has been observed that individuals with PHT have a higher risk of developing hypertension later in life. Therefore, it is important to understand the mechanisms contributing to PHT in order to possibly prevent hypertension. Omega-3 fatty acids found in fish oils have been suggested as a means of lowering blood pressure. However, little is known on the effects of fish oil in PHT humans. Therefore we conducted two studies. In Study 1 we investigated PHT and normotensive (NT) individuals during a mental stress task. Mental stress is known to contribute to the development of hypertension. In Study 2 PHT and NT subjects were placed in an eight week double-blind placebo controlled study in which subjects consumed 9g/day of either fish oil or placebo (olive oil) in addition to their regular diets. Subjects were tested during a resting baseline (seated and supine), 5 minutes of a mental stress task, and 5 minutes of recovery both pre and post supplementation. We measured arterial pressure (AP), heart rate (HR), muscle sympathetic nerve activity (MSNA), and forearm and calf vascular responses. In Study 1 PHT demonstrated augmented AP and blunted vasodilation during mental stress, but MSNA did not change. In Study 2, fish oil did not directly influence blood pressure, MSNA or vascular responses to mental stress. However, it became clear that fish oil had an effect on some but not all subjects (both PHT and NT). Specifically, subjects who experienced a reduced blood pressure response to fish oil also demonstrated a decrease in MSNA and HR during mental stress. Collectively, the investigations in this dissertation had several novel findings. First, PHT individuals demonstrate an augmented pressor and blunted vascular response to mental stress, a response that may be contributing to the development of hypertension. Second, fish oil does not consistently lower resting blood pressure, but the interindividual responses may be related to MSNA. Third, fish oil attenuated the heart rate and MSNA responses and to mental stress in both PHT and NT. In conclusion, we found that there are both similarities and differences in the way PHT and NT individuals respond to mental stress and fish oil. Advisors/Committee Members: Jason Robert Carter.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Schwartz, C. E. (2011). Omega-3 fatty acids and the neurovascular responses to mental stress in humans. (Doctoral Dissertation). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/194

Chicago Manual of Style (16^th Edition):

Schwartz, Christopher Elmer. “Omega-3 fatty acids and the neurovascular responses to mental stress in humans.” 2011. Doctoral Dissertation, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/194.

MLA Handbook (7^th Edition):

Schwartz, Christopher Elmer. “Omega-3 fatty acids and the neurovascular responses to mental stress in humans.” 2011. Web. 25 Jan 2020.

Vancouver:

Schwartz CE. Omega-3 fatty acids and the neurovascular responses to mental stress in humans. [Internet] [Doctoral dissertation]. Michigan Technological University; 2011. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/194.

Council of Science Editors:

Schwartz CE. Omega-3 fatty acids and the neurovascular responses to mental stress in humans. [Doctoral Dissertation]. Michigan Technological University; 2011. Available from: http://digitalcommons.mtu.edu/etds/194

Michigan Technological University

20. Stream, Sarah F. Acute alcohol ingestion and sympathetic neural responses during orthostatic stress in humans.

Degree: MS, Department of Biological Sciences, 2012, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/185

► Acute alcohol consumption has been reported to decrease mean arterial pressure (MAP) during orthostatic challenge, a response that may contribute to alcohol-mediated hypotension and… (more)

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Stream, S. F. (2012). Acute alcohol ingestion and sympathetic neural responses during orthostatic stress in humans. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/185

Chicago Manual of Style (16^th Edition):

Stream, Sarah F. “Acute alcohol ingestion and sympathetic neural responses during orthostatic stress in humans.” 2012. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/185.

MLA Handbook (7^th Edition):

Stream, Sarah F. “Acute alcohol ingestion and sympathetic neural responses during orthostatic stress in humans.” 2012. Web. 25 Jan 2020.

Vancouver:

Stream SF. Acute alcohol ingestion and sympathetic neural responses during orthostatic stress in humans. [Internet] [Masters thesis]. Michigan Technological University; 2012. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/185.

Council of Science Editors:

Stream SF. Acute alcohol ingestion and sympathetic neural responses during orthostatic stress in humans. [Masters Thesis]. Michigan Technological University; 2012. Available from: http://digitalcommons.mtu.edu/etds/185

Michigan Technological University

21. Vogel, Kara R. Inhibition of L-type amino acid transport with non-physiological amino acids in the Pahenu2 mouse model of phenylketonuria.

Degree: MS, Department of Biological Sciences, 2012, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/193

► Phenylketonuria, an autosomal recessive Mendelian disorder, is one of the most common inborn errors of metabolism. Although currently treated by diet, many suboptimal outcomes… (more)

▼ Phenylketonuria, an autosomal recessive Mendelian disorder, is one of the most common inborn errors of metabolism. Although currently treated by diet, many suboptimal outcomes occur for patients. Neuropathological outcomes include cognitive loss, white matter abnormalities, and hypo- or demyelination, resulting from high concentrations and/or fluctuating levels of phenylalanine. High phenylalanine can also result in competitive exclusion of other large neutral amino acids from the brain, including tyrosine and tryptophan (essential precursors of dopamine and serotonin). This competition occurs at the blood brain barrier, where the L-type amino acid transporter, LAT1, selectively facilitates entry of large neutral amino acids. The hypothesis of these studies is that certain non-physiological amino acids (NPAA; DL-norleucine (NL), 2-aminonorbornane (NB; 2-aminobicyclo-(2,1,1)-heptane-2-carboxylic acid), α-aminoisobutyrate (AIB), and α-methyl-aminoisobutyrate (MAIB)) would competitively inhibit LAT1 transport of phenylalanine (Phe) at the blood-brain barrier interface. To test this hypothesis, Pah-/- mice (n=5, mixed gender; Pah+/-(n=5) as controls) were fed either 5% NL, 0.5% NB, 5% AIB or 3% MAIB (w/w 18% protein mouse chow) for 3 weeks. Outcome measurements included food intake, body weight, brain LNAAs, and brain monoamines measured via LCMS/MS or HPLC. Brain Phe values at sacrifice were significantly reduced for NL, NB, and MAIB, verifying the hypothesis that these NPAAs could inhibit Phe trafficking into the brain. However, concomitant reductions in tyrosine and methionine occurred at the concentrations employed. Blood Phe levels were not altered indicating no effect of NPAA competitors in the gut. Brain NL and NB levels, measured with HPLC, verified both uptake and transport of NPAAs. Although believed predominantly unmetabolized, NL feeding significantly increased blood urea nitrogen. Pah-/-disturbances of monoamine metabolism were exacerbated by NPAA intervention, primarily with NB (the prototypical LAT inhibitor). To achieve the overarching goal of using NPAAs to stabilize Phe transport levels into the brain, a specific Phe-reducing combination and concentration of NPAAs must be found. Our studies represent the first in vivo use of NL, NB and MAIB in Pah-/- mice, and provide proof-of-principle for further characterization of these LAT inhibitors. Our data is the first to document an effect of MAIB, a specific system A transport inhibitor, on large neutral amino acid transport. Advisors/Committee Members: Kenneth Michael Gibson.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Vogel, K. R. (2012). Inhibition of L-type amino acid transport with non-physiological amino acids in the Pahenu2 mouse model of phenylketonuria. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/193

Chicago Manual of Style (16^th Edition):

Vogel, Kara R. “Inhibition of L-type amino acid transport with non-physiological amino acids in the Pahenu2 mouse model of phenylketonuria.” 2012. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/193.

MLA Handbook (7^th Edition):

Vogel, Kara R. “Inhibition of L-type amino acid transport with non-physiological amino acids in the Pahenu2 mouse model of phenylketonuria.” 2012. Web. 25 Jan 2020.

Vancouver:

Vogel KR. Inhibition of L-type amino acid transport with non-physiological amino acids in the Pahenu2 mouse model of phenylketonuria. [Internet] [Masters thesis]. Michigan Technological University; 2012. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/193.

Council of Science Editors:

Vogel KR. Inhibition of L-type amino acid transport with non-physiological amino acids in the Pahenu2 mouse model of phenylketonuria. [Masters Thesis]. Michigan Technological University; 2012. Available from: http://digitalcommons.mtu.edu/etds/193

Michigan Technological University

22. Kiemle, Sarah Nelson. Extracellular matrix of the charophycean green algae.

Degree: PhD, Department of Biological Sciences, 2010, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/190

► A comprehensive knowledge of cell wallstructure and function throughout the plant kingdom is essential to understanding cell wall evolution. The fundamental understanding of the… (more)

▼ A comprehensive knowledge of cell wallstructure and function throughout the plant kingdom is essential to understanding cell wall evolution. The fundamental understanding of the charophycean green algal cell wall is broadening. The similarities and differences that exist between land plant and algal cell walls provide opportunities to understand plant evolution. A variety of polymers previously associated with higher plants were discovered in the charophycean green algae (CGA), including homogalacturonans, cross-linking glycans, arabinogalactan protein, β-glucans, and cellulose. The cellulose content of CGA cell walls ranged from 6% to 43%, with the higher valuescomparable to that found in the primary cell wall of land plants (20-30%). (1,3)β-glucans were found in the unicellular Chlorokybus atmophyticus, Penium margaritaceum, and Cosmarium turpini, the unbranched filamentous Klebsormidium flaccidum, and the multicellular Chara corallina. The discovery of homogalacturonan in Penium margaritaceum representsthe first confirmation of land plant-type pectinsin desmids and the second rigorous characterization of a pectin polymer from the charophycean algae. Homogalacturonan was also indicated from the basal species Chlorokybus atmophyticus and Klebsormidium flaccidum. There is evidence of branched pectins in Cosmarium turpini and linkage analysis suggests the presence of type I rhamnogalacturonan (RGI). Cross-linking β-glucans are associated with cellulose microfibrils during land plant cell growth, and were found in the cell wall of CGA. The evidence of mixed-linkage glucan (MLG) in the 11 charophytesis both suprising and significant given that MLG was once thought to be specific to some grasses. The organization and structure of Cosmarium turpini and Chara corallina MLG was found to be similar to that of Equisetumspp., whereas the basal species of the CGA, Chlorokybus atmophyticus and Klebsormidium flaccidum, have unique organization of alternating of 3- and 4-linkages. The significance of this result on the evolution of the MLG synthetic pathway has yet to be determined. The extracellular matrix (ECM) of Chlorokybus atmophyticus, Klebsormidium flaccidum, and Spirogyra spp. exhibits significant biochemical diversity, ranging from distinct “land plant” polymers to polysaccharides unique to these algae. The neutral sugar composition of Chlorokybus atmophyticus hot water extract and Spirogyra extracellular polymeric substance (EPS), combined with antibody labeling results, revealed the distinct possibility of an arabinogalactan protein in these organisms. Polysaccharide analysis of Zygnematales (desmid) EPS, indicated a probable range of different EPS backbones and substitution patterns upon the core portions of the molecules. Desmid EPS is predominately composed of a complex matrix of branched, uronic acid containing polysaccharides with ester… Advisors/Committee Members: Michael R Gretz.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Kiemle, S. N. (2010). Extracellular matrix of the charophycean green algae. (Doctoral Dissertation). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/190

Chicago Manual of Style (16^th Edition):

Kiemle, Sarah Nelson. “Extracellular matrix of the charophycean green algae.” 2010. Doctoral Dissertation, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/190.

MLA Handbook (7^th Edition):

Kiemle, Sarah Nelson. “Extracellular matrix of the charophycean green algae.” 2010. Web. 25 Jan 2020.

Vancouver:

Kiemle SN. Extracellular matrix of the charophycean green algae. [Internet] [Doctoral dissertation]. Michigan Technological University; 2010. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/190.

Council of Science Editors:

Kiemle SN. Extracellular matrix of the charophycean green algae. [Doctoral Dissertation]. Michigan Technological University; 2010. Available from: http://digitalcommons.mtu.edu/etds/190

Michigan Technological University

23. Forgette, Kyle D. Relationship between instream habitat characteristics, emergent insects, and riparian bird communities.

Degree: MS, Department of Biological Sciences, 2011, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/196

► Streams and riparian areas can be intricately connected via physical and biotic interactions that influence habitat conditions and supply resource subsidies between these ecosystems.… (more)

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Forgette, K. D. (2011). Relationship between instream habitat characteristics, emergent insects, and riparian bird communities. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/196

Chicago Manual of Style (16^th Edition):

Forgette, Kyle D. “Relationship between instream habitat characteristics, emergent insects, and riparian bird communities.” 2011. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/196.

MLA Handbook (7^th Edition):

Forgette, Kyle D. “Relationship between instream habitat characteristics, emergent insects, and riparian bird communities.” 2011. Web. 25 Jan 2020.

Vancouver:

Forgette KD. Relationship between instream habitat characteristics, emergent insects, and riparian bird communities. [Internet] [Masters thesis]. Michigan Technological University; 2011. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/196.

Council of Science Editors:

Forgette KD. Relationship between instream habitat characteristics, emergent insects, and riparian bird communities. [Masters Thesis]. Michigan Technological University; 2011. Available from: http://digitalcommons.mtu.edu/etds/196

Michigan Technological University

24. Witting, Jennifer L. Effects of fixed night shift work on 24 hour blood pressure regulation, state anxiety levels and total sleep time.

Degree: MS, Department of Biological Sciences, 2011, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/188

► Nearly 22 million Americans operate as shift workers, and shift work has been linked to the development of cardiovascular disease (CVD). This study is… (more)

▼ Nearly 22 million Americans operate as shift workers, and shift work has been linked to the development of cardiovascular disease (CVD). This study is aimed at identifying pivotal risk factors of CVD by assessing 24 hour ambulatory blood pressure, state anxiety levels and sleep patterns in 12 hour fixed shift workers. We hypothesized that night shift work would negatively affect blood pressure regulation, anxiety levels and sleep patterns. A total of 28 subjects (ages 22-60) were divided into two groups: 12 hour fixed night shift workers (n=15) and 12 hour fixed day shift workers (n=13). 24 hour ambulatory blood pressure measurements (Space Labs 90207) were taken twice: once during a regular work day and once on a non-work day. State anxiety levels were assessed on both test days using the Speilberger’s State Trait Anxiety Inventory. Total sleep time (TST) was determined using self recorded sleep diary. Night shift workers demonstrated increases in 24 hour systolic (122 ± 2 to 126 ± 2 mmHg, P=0.012); diastolic (75 ± 1 to 79 ± 2 mmHg, P=0.001); and mean arterial pressures (90 ± 2 to 94 ± 2mmHg, P<0.001) during work days compared to off days. In contrast, 24 hour blood pressures were similar during work and off days in day shift workers. Night shift workers reported less TST on work days versus off days (345 ± 16 vs. 552 ± 30 min; P<0.001), whereas day shift workers reported similar TST during work and off days (475 ± 16 minutes to 437 ± 20 minutes; P=0.231). State anxiety scores did not differ between the groups or testing days (time*group interaction P=0.248), suggesting increased 24 hour blood pressure during night shift work is related to decreased TST, not short term anxiety. Our findings suggest that fixed night shift work causes disruption of the normal sleep-wake cycle negatively affecting acute blood pressure regulation, which may increase the long-term risk for CVD. Advisors/Committee Members: Jason Robert Carter, John James Durocher.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Witting, J. L. (2011). Effects of fixed night shift work on 24 hour blood pressure regulation, state anxiety levels and total sleep time. (Masters Thesis). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/188

Chicago Manual of Style (16^th Edition):

Witting, Jennifer L. “Effects of fixed night shift work on 24 hour blood pressure regulation, state anxiety levels and total sleep time.” 2011. Masters Thesis, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/188.

MLA Handbook (7^th Edition):

Witting, Jennifer L. “Effects of fixed night shift work on 24 hour blood pressure regulation, state anxiety levels and total sleep time.” 2011. Web. 25 Jan 2020.

Vancouver:

Witting JL. Effects of fixed night shift work on 24 hour blood pressure regulation, state anxiety levels and total sleep time. [Internet] [Masters thesis]. Michigan Technological University; 2011. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/188.

Council of Science Editors:

Witting JL. Effects of fixed night shift work on 24 hour blood pressure regulation, state anxiety levels and total sleep time. [Masters Thesis]. Michigan Technological University; 2011. Available from: http://digitalcommons.mtu.edu/etds/188

Michigan Technological University

25. Winder, Eric Michael. Bacteriorhodopsin protein hybrids for chemical and biological sensing.

Degree: PhD, Department of Biological Sciences, 2010, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etd-restricted/2

► Bacteriorhodopsin (bR), an optoelectric protein found in Halobacterium salinarum, has the potential for use in protein hybrid sensing systems. Bacteriorhodopsin has no intrinsic sensing… (more)

▼ Bacteriorhodopsin (bR), an optoelectric protein found in Halobacterium salinarum, has the potential for use in protein hybrid sensing systems. Bacteriorhodopsin has no intrinsic sensing properties, however molecular and chemical tools permit production of bR protein hybrids with transducing and sensing properties. As a proof of concept, a maltose binding protein-bacteriorhodopsin ([MBP]-bR) hybrid was developed. It was proposed that the energy associated with target molecule binding, maltose, to the hybrid sensor protein would provide a means to directly modulate the electrical output from the MBP-bR bio-nanosensor platform. The bR protein hybrid is produced by linkage between bR (principal component of purified purple membrane [PM]) and MBP, which was produced by use of a plasmid expression vector system in Escherichia coli and purified utilizing an amylose affinity column. These proteins were chemically linked using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), which facilitates formation of an amide bond between a primary carboxylic acid and a primary amine. The presence of novel protein hybrids after chemical linkage was analyzed by SDSPAGE. Soluble proteins (MBP-only derivatives and unlinked MBP) were separated from insoluble proteins (PM derivatives and unlinked PM) using size exclusion chromatography. The putatively identified MBP-bR protein hybrid, in addition to unlinked bR, was collected. This sample was normalized for bR concentration to native PM and both were deposited onto indium tin oxide (ITO) coated glass slides by electrophoretic sedimentation. The photoresponse of both samples, activated using 100 Watt tungsten lamp at 10 cm distance, were equal at 175 mV. Testing of deposited PM with 1 mM sucrose or 1 mM maltose showed no change in the photoresponse of the xiv material, however addition of 1 mM maltose to the deposited MBP-bR linked hybrid material elicited a 57% decrease in photoresponse indicating a positive response for targeting of maltose. This chemically linked MBP-bR hybrid protein, with bacteriorhodopsin, as a photoresponsive transducing substrate, shows promise for creation of a universal sensing array by attachment of other pertinent sensing materials, in lieu of the maltose binding protein utilized. This strategy would allow significant reduction in sensor size, while increasing responsiveness and sensitivity at nano and picomolar levels. Advisors/Committee Members: John H Adler, Craig R Friedrich.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Winder, E. M. (2010). Bacteriorhodopsin protein hybrids for chemical and biological sensing. (Doctoral Dissertation). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etd-restricted/2

Chicago Manual of Style (16^th Edition):

Winder, Eric Michael. “Bacteriorhodopsin protein hybrids for chemical and biological sensing.” 2010. Doctoral Dissertation, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etd-restricted/2.

MLA Handbook (7^th Edition):

Winder, Eric Michael. “Bacteriorhodopsin protein hybrids for chemical and biological sensing.” 2010. Web. 25 Jan 2020.

Vancouver:

Winder EM. Bacteriorhodopsin protein hybrids for chemical and biological sensing. [Internet] [Doctoral dissertation]. Michigan Technological University; 2010. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etd-restricted/2.

Council of Science Editors:

Winder EM. Bacteriorhodopsin protein hybrids for chemical and biological sensing. [Doctoral Dissertation]. Michigan Technological University; 2010. Available from: http://digitalcommons.mtu.edu/etd-restricted/2

Michigan Technological University

26. Krom, Nicholas D. Arrangement of gene pairs, retrotransposon insertions, and regulation of gene expression in plants.

Degree: PhD, Department of Biological Sciences, 2009, Michigan Technological University

URL: http://digitalcommons.mtu.edu/etds/707

► Plant genomes are extremely complex. Myriad factors contribute to their evolution and organization, as well as to the expression and regulation of individual genes.… (more)

▼ Plant genomes are extremely complex. Myriad factors contribute to their evolution and organization, as well as to the expression and regulation of individual genes. Here we present investigations into several such factors and their influence on genome structure and gene expression: the arrangement of pairs of physically adjacent genes, retrotransposons closely associated with genes, and the effect of retrotransposons on gene pair evolution. All sequenced plant genomes contain a significant fraction of retrotransposons, including that of rice. We investigated the effects of retrotransposons within rice genes and within a 1 kb putative promoter region upstream of each gene. We found that approximately one-sixth of all rice genes are closely associated with retrotransposons. Insertions within a gene’s promoter region tend to block gene expression, while retrotransposons within genes promote the existence of alternative splicing forms. We also identified several other trends in retrotransposon insertion and its effects on gene expression. Several studies have previously noted a connection among genes between physical proximity and correlated expression profiles. To determine the degree to which this correlation depends on an exact physical arrangement, we studied the expression and interspecies conservation of convergent and divergent gene pairs in rice, Arabidopsis, and Populus trichocarpa. Correlated expression among gene pairs was quite common in all three species, yet conserved arrangement was rare. However, conservation of gene pair arrangement was significantly more common among pairs with strongly correlated expression levels. In order to uncover additional properties of gene pair conservation and rearrangement, we performed a comparative analysis of convergent, divergent, and tandem gene pairs in rice, sorghum, maize, and Brachypodium. We noted considerable differences between gene pair types and species. We also constructed a putative evolutionary history for each pair, which led to several interesting discoveries. To further elucidate the causes of gene pair conservation and rearrangement, we identified retrotransposon insertions in and near rice gene pairs. Retrotransposon-associated pairs are less likely to be conserved, although there are significant differences in the possible effect of different types and locations of retrotransposon insertions. The three types of gene pair also varied in their susceptibility to retrotransposon-associated evolutionary changes. Advisors/Committee Members: Ramakrishna Wusirika.

Subjects/Keywords: Biology; Life Sciences

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APA (6^th Edition):

Krom, N. D. (2009). Arrangement of gene pairs, retrotransposon insertions, and regulation of gene expression in plants. (Doctoral Dissertation). Michigan Technological University. Retrieved from http://digitalcommons.mtu.edu/etds/707

Chicago Manual of Style (16^th Edition):

Krom, Nicholas D. “Arrangement of gene pairs, retrotransposon insertions, and regulation of gene expression in plants.” 2009. Doctoral Dissertation, Michigan Technological University. Accessed January 25, 2020. http://digitalcommons.mtu.edu/etds/707.

MLA Handbook (7^th Edition):

Krom, Nicholas D. “Arrangement of gene pairs, retrotransposon insertions, and regulation of gene expression in plants.” 2009. Web. 25 Jan 2020.

Vancouver:

Krom ND. Arrangement of gene pairs, retrotransposon insertions, and regulation of gene expression in plants. [Internet] [Doctoral dissertation]. Michigan Technological University; 2009. [cited 2020 Jan 25]. Available from: http://digitalcommons.mtu.edu/etds/707.

Council of Science Editors:

Krom ND. Arrangement of gene pairs, retrotransposon insertions, and regulation of gene expression in plants. [Doctoral Dissertation]. Michigan Technological University; 2009. Available from: http://digitalcommons.mtu.edu/etds/707

University of South Carolina

27. Sreeram Chellappa, Geetha Saarunya. CBK Bacteriophage Genome Annotation and Organization.

Degree: MS, Biological Sciences, 2010, University of South Carolina

URL: https://scholarcommons.sc.edu/etd/411

► Caulobacter bacteriophages have been isolated from local ponds, streams and the aquaria of fish stores. About three-fourths of these phages belonged to the B3… (more)

Subjects/Keywords: Biology; Life Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sreeram Chellappa, G. S. (2010). CBK Bacteriophage Genome Annotation and Organization. (Masters Thesis). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/411

Chicago Manual of Style (16^th Edition):

Sreeram Chellappa, Geetha Saarunya. “CBK Bacteriophage Genome Annotation and Organization.” 2010. Masters Thesis, University of South Carolina. Accessed January 25, 2020. https://scholarcommons.sc.edu/etd/411.

MLA Handbook (7^th Edition):

Sreeram Chellappa, Geetha Saarunya. “CBK Bacteriophage Genome Annotation and Organization.” 2010. Web. 25 Jan 2020.

Vancouver:

Sreeram Chellappa GS. CBK Bacteriophage Genome Annotation and Organization. [Internet] [Masters thesis]. University of South Carolina; 2010. [cited 2020 Jan 25]. Available from: https://scholarcommons.sc.edu/etd/411.

Council of Science Editors:

Sreeram Chellappa GS. CBK Bacteriophage Genome Annotation and Organization. [Masters Thesis]. University of South Carolina; 2010. Available from: https://scholarcommons.sc.edu/etd/411

28. Ingels, Ben. THE EFFECT OF ARGININE VASOTOCIN ON CRYOPROTECTANT PRODUCTION DURING FREEZING IN THE PACIFIC CHORUS FROG, Pseudacris regilla.

Degree: MS, Biology, 2017, Central Washington University

URL: https://digitalcommons.cwu.edu/etd/599

► Freeze Tolerance is an adaptation seen amongst several species of amphibians as a way of surviving during wintering months. Use of cryoprotectants is seen… (more)

Subjects/Keywords: Biology; Life Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ingels, B. (2017). THE EFFECT OF ARGININE VASOTOCIN ON CRYOPROTECTANT PRODUCTION DURING FREEZING IN THE PACIFIC CHORUS FROG, Pseudacris regilla. (Masters Thesis). Central Washington University. Retrieved from https://digitalcommons.cwu.edu/etd/599

Chicago Manual of Style (16^th Edition):

Ingels, Ben. “THE EFFECT OF ARGININE VASOTOCIN ON CRYOPROTECTANT PRODUCTION DURING FREEZING IN THE PACIFIC CHORUS FROG, Pseudacris regilla.” 2017. Masters Thesis, Central Washington University. Accessed January 25, 2020. https://digitalcommons.cwu.edu/etd/599.

MLA Handbook (7^th Edition):

Ingels, Ben. “THE EFFECT OF ARGININE VASOTOCIN ON CRYOPROTECTANT PRODUCTION DURING FREEZING IN THE PACIFIC CHORUS FROG, Pseudacris regilla.” 2017. Web. 25 Jan 2020.

Vancouver:

Ingels B. THE EFFECT OF ARGININE VASOTOCIN ON CRYOPROTECTANT PRODUCTION DURING FREEZING IN THE PACIFIC CHORUS FROG, Pseudacris regilla. [Internet] [Masters thesis]. Central Washington University; 2017. [cited 2020 Jan 25]. Available from: https://digitalcommons.cwu.edu/etd/599.

Council of Science Editors:

Ingels B. THE EFFECT OF ARGININE VASOTOCIN ON CRYOPROTECTANT PRODUCTION DURING FREEZING IN THE PACIFIC CHORUS FROG, Pseudacris regilla. [Masters Thesis]. Central Washington University; 2017. Available from: https://digitalcommons.cwu.edu/etd/599

Seton Hall University

29. Beaury, Michael W. Confocal Characterization of the Apical Testes and Ultrastructural Analysis of the Seminal Visicles of Drosophila pseudoobscura.

Degree: MS, Biological Science, 2012, Seton Hall University

URL: http://scholarship.shu.edu/theses/257

► The Drosophila genus contains thousands of different species. Drosophila melanogaster is the most popular Drosophila species used in biological research. Drosophila pseudoobscura is the… (more)

Subjects/Keywords: Biology; Life Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Beaury, M. W. (2012). Confocal Characterization of the Apical Testes and Ultrastructural Analysis of the Seminal Visicles of Drosophila pseudoobscura. (Masters Thesis). Seton Hall University. Retrieved from http://scholarship.shu.edu/theses/257

Chicago Manual of Style (16^th Edition):

Beaury, Michael W. “Confocal Characterization of the Apical Testes and Ultrastructural Analysis of the Seminal Visicles of Drosophila pseudoobscura.” 2012. Masters Thesis, Seton Hall University. Accessed January 25, 2020. http://scholarship.shu.edu/theses/257.

MLA Handbook (7^th Edition):

Beaury, Michael W. “Confocal Characterization of the Apical Testes and Ultrastructural Analysis of the Seminal Visicles of Drosophila pseudoobscura.” 2012. Web. 25 Jan 2020.

Vancouver:

Beaury MW. Confocal Characterization of the Apical Testes and Ultrastructural Analysis of the Seminal Visicles of Drosophila pseudoobscura. [Internet] [Masters thesis]. Seton Hall University; 2012. [cited 2020 Jan 25]. Available from: http://scholarship.shu.edu/theses/257.

Council of Science Editors:

Beaury MW. Confocal Characterization of the Apical Testes and Ultrastructural Analysis of the Seminal Visicles of Drosophila pseudoobscura. [Masters Thesis]. Seton Hall University; 2012. Available from: http://scholarship.shu.edu/theses/257

30. Buckland, Brooke A. Role of CaMKII in Pancreatic Alpha cells.

Degree: MS, Biological Sciences, 2013, U of Denver

URL: https://digitalcommons.du.edu/etd/96

► Calcium/calmodulin-dependent protein kinase II (CaMKII) has been identified as an important modulator in controlling electrical activity in neurons and the heart; however, a role… (more)

Subjects/Keywords: Biology; Life Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Buckland, B. A. (2013). Role of CaMKII in Pancreatic Alpha cells. (Thesis). U of Denver. Retrieved from https://digitalcommons.du.edu/etd/96

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Buckland, Brooke A. “Role of CaMKII in Pancreatic Alpha cells.” 2013. Thesis, U of Denver. Accessed January 25, 2020. https://digitalcommons.du.edu/etd/96.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Buckland, Brooke A. “Role of CaMKII in Pancreatic Alpha cells.” 2013. Web. 25 Jan 2020.

Vancouver:

Buckland BA. Role of CaMKII in Pancreatic Alpha cells. [Internet] [Thesis]. U of Denver; 2013. [cited 2020 Jan 25]. Available from: https://digitalcommons.du.edu/etd/96.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Buckland BA. Role of CaMKII in Pancreatic Alpha cells. [Thesis]. U of Denver; 2013. Available from: https://digitalcommons.du.edu/etd/96

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations