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Delft University of Technology
1.
Groutars, Eduard (author).
Bacterial Composites.
Degree: 2020, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:0a719ec0-85de-49d8-a959-5f84cac86a1b 
► This project started with analyzing the different roles and potentials that bacteria have for the growth of new materials with ecological benefits. Here questions arise…
(more)
▼ This project started with analyzing the different roles and potentials that bacteria have for the growth of new materials with ecological benefits. Here questions arise about the role that a designer can fulfill in the development of such novel materials. What new skillset does he/she need to obtain? How will a collaboration with biologists take place? In order to get a better understanding of the potentials of materials grown by bacteria and the subsequent role of a designer, a Material Driven Design (Karana et al., 2015) project was performed in collaboration with scientists from the Aubin-Tam research group, part of the Bionanoscience department of the Delft University of Technology. The starting point of the design project was a composite material consisting of three ingredients that are grown by three separate species of bacteria. In order to gain an understanding of how this material was grown and produced, the designer performed a plethora of experiments investigating; the growth of the organisms and the amount of material they produced; how the ratio between the three ingredients influenced the resulting material; how the way in which the material was processed resulted in its final form and properties. This led to the understanding that this material is highly programmable in its form and properties such as its flexibility, strength and surface roughness. With this in mind, user studies were performed in which it was found that the versatility of the material was considered interesting and intriguing by participants. They wonder what it is and how it is made, finding it hard to believe that bacteria grew such a material. This led to a material concept in which the designer proposes to play with these varying properties of the material, resulting in contrasting material experiences and highlighting the material its ability to appear as something that is both natural and man-made at the same time. This was done by exploring various processesing potentials of the material and analysing how different parameters of these processes influence the resulting material its properties. In doing so, the designer provided a framework by which future designers can program and explore this bio-based material that shows a lot of different potentials.
Advisors/Committee Members: Karana, Elvin (mentor), Aubin-Tam, Marie-eve (mentor), Delft University of Technology (degree granting institution).
Subjects/Keywords: Biofabrication; Bacteria; Composites
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APA (6th Edition):
Groutars, E. (. (2020). Bacterial Composites. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:0a719ec0-85de-49d8-a959-5f84cac86a1b
Chicago Manual of Style (16th Edition):
Groutars, Eduard (author). “Bacterial Composites.” 2020. Masters Thesis, Delft University of Technology. Accessed January 24, 2021.
http://resolver.tudelft.nl/uuid:0a719ec0-85de-49d8-a959-5f84cac86a1b.
MLA Handbook (7th Edition):
Groutars, Eduard (author). “Bacterial Composites.” 2020. Web. 24 Jan 2021.
Vancouver:
Groutars E(. Bacterial Composites. [Internet] [Masters thesis]. Delft University of Technology; 2020. [cited 2021 Jan 24].
Available from: http://resolver.tudelft.nl/uuid:0a719ec0-85de-49d8-a959-5f84cac86a1b.
Council of Science Editors:
Groutars E(. Bacterial Composites. [Masters Thesis]. Delft University of Technology; 2020. Available from: http://resolver.tudelft.nl/uuid:0a719ec0-85de-49d8-a959-5f84cac86a1b
Universiteit Utrecht
2.
Goversen, B.
Mechanics in articular cartilage regeneration.
Degree: 2015, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/307805
► Articular cartilage is an avascular load-bearing tissue lining the surface of long bones where it serves for the absorbance of shocks as well as the…
(more)
▼ Articular cartilage is an avascular load-bearing tissue lining the surface of long bones where it serves for the absorbance of shocks as well as the lubrication of joints. Treatments to repair cartilage defects mainly consist of cell therapies, which do not yield biomechanically sound tissue. Therefore tissue engineering has been proposed as a viable alternative. In order to meet biomechanical demands tissue engineered constructs require specific architecture, which could be accomplished by 3D deposition of hydrogels. Unfortunately, hydrogels are not mechanically compatible with native articular cartilage. The use of reinforced hydrogels in
biofabrication allows tailoring of mechanical properties with a retained biocompatibility. Mechanical loading in a bioreactor can contribute to the improvement of tissue engineered constructs by mimicking in vivo conditions. Mechanical tuning in
biofabrication as well as mechanical training could contribute to a clinically applicable tissue.
Advisors/Committee Members: Malda, J.
Subjects/Keywords: Cartilage; tissue engineering; mechanics; biofabrication
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APA (6th Edition):
Goversen, B. (2015). Mechanics in articular cartilage regeneration. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/307805
Chicago Manual of Style (16th Edition):
Goversen, B. “Mechanics in articular cartilage regeneration.” 2015. Masters Thesis, Universiteit Utrecht. Accessed January 24, 2021.
http://dspace.library.uu.nl:8080/handle/1874/307805.
MLA Handbook (7th Edition):
Goversen, B. “Mechanics in articular cartilage regeneration.” 2015. Web. 24 Jan 2021.
Vancouver:
Goversen B. Mechanics in articular cartilage regeneration. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Jan 24].
Available from: http://dspace.library.uu.nl:8080/handle/1874/307805.
Council of Science Editors:
Goversen B. Mechanics in articular cartilage regeneration. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/307805
Clemson University
3.
Jordan, Holly.
Bioprinting as a Tool to Evaluate Interactions of Cancerous and Noncancerous Cells.
Degree: MS, Bioengineering, 2015, Clemson University
URL: https://tigerprints.clemson.edu/all_theses/2229
► Cancer is becoming one the leading causes of death worldwide, and in particular, breast cancer, which is the second highest cause of cancer death for…
(more)
▼ Cancer is becoming one the leading causes of death worldwide, and in particular, breast cancer, which is the second highest cause of cancer death for women. Approximately 12 percent of US women will develop invasive breast cancer, and about 40,000 of US women will die from breast cancer in 2015. With better detection and treatment options, breast cancer death has been decreasing over the past two decades. Despite declining rates in breast cancer death, cancer progression is not well understood. There are many studies focused on cancer, but in situ cancer studies are often hard to reproduce and can even be impractical. There is a demand for in vitro cancer models that imitate the in vivo environment of cancerous cells and tumor development. Numerous models have been developed to better understand normal and cancerous breast tissue. Tissue engineering involves the generation of three-dimensional (3D) tissue structures by seeding cells onto a scaffold so the cells can attach and proliferate into a 3D functional tissue. Unfortunately this approach lacks precise cellular placement and fails to create the intricate and complex environment of normal human tissue. The specific microenvironment has been shown to play a key role in metastatic cell behavior and in determining phenotype and function of mammary cells. Design of a particular 3D arrangement of cells would allow a better understanding of cellular behavior and interactions. One promising technique for tissue formation is
biofabrication, which can generate 3D tissues through the delivery of cells and biomaterials layer-by-layer.
Biofabrication can precisely arrange cells and create scaffolds with more organization and complexity. Bioprinting is the drop-by-drop deposition of cells and biomaterials. Inkjet printing technology has been used to create bioprinters and is an inexpensive way to print precise patterns of cell and biomaterials with little reduction in cellular viability, with easy pattern modification, and with minimal effect on the substrate. Inkjet bioprinting has great potential for the development of in vitro breast tissue models; the general aim of this thesis was to test the capabilities of inkjet bioprinting for creating in vitro cancer models. The objective of this work was to characterize the interactions of cancerous and noncancerous breast cells through several qualitative and quantitative methods after the cells were printed into lines of varying distances apart, using a modified inkjet printer as a bioprinter. MCF-10a and MCF-7 cells were printed into two opposing lines of varying distances apart onto a collagen coated glass slide, using a bioprinter. To assess the effect of the distance on printed lines of cancer and noncancerous breast cells, several testing methods were proposed, and samples were taken at time points of Day 1 and Day 5 after printing. The results from the collected data lead to several general, key findings. First, the cancerous cells modified the cellular behavior of the noncancerous…
Advisors/Committee Members: Burg, Dr. Karen J.L., LaBerge, Dr. Martine, Burg, Dr. Timothy C..
Subjects/Keywords: Biofabrication; Bioprinting; Breast Cancer; Engineering
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APA (6th Edition):
Jordan, H. (2015). Bioprinting as a Tool to Evaluate Interactions of Cancerous and Noncancerous Cells. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/2229
Chicago Manual of Style (16th Edition):
Jordan, Holly. “Bioprinting as a Tool to Evaluate Interactions of Cancerous and Noncancerous Cells.” 2015. Masters Thesis, Clemson University. Accessed January 24, 2021.
https://tigerprints.clemson.edu/all_theses/2229.
MLA Handbook (7th Edition):
Jordan, Holly. “Bioprinting as a Tool to Evaluate Interactions of Cancerous and Noncancerous Cells.” 2015. Web. 24 Jan 2021.
Vancouver:
Jordan H. Bioprinting as a Tool to Evaluate Interactions of Cancerous and Noncancerous Cells. [Internet] [Masters thesis]. Clemson University; 2015. [cited 2021 Jan 24].
Available from: https://tigerprints.clemson.edu/all_theses/2229.
Council of Science Editors:
Jordan H. Bioprinting as a Tool to Evaluate Interactions of Cancerous and Noncancerous Cells. [Masters Thesis]. Clemson University; 2015. Available from: https://tigerprints.clemson.edu/all_theses/2229
University of Wollongong
4.
Ferris, Cameron.
Bio-inks for drop-on-demand cell printing.
Degree: PhD, 2013, University of Wollongong
URL: 090301
Biomaterials,
090304
Medical
Devices,
100404
Regenerative
Medicine
(incl.
Stem
Cells
and
Tissue
Engineering)
;
https://ro.uow.edu.au/theses/3875
► A rapidly growing synergy between biological science and engineering technology is currently re-shaping the way we view the challenge of treating injury and disease.…
(more)
▼ A rapidly growing synergy between biological science and engineering technology is currently re-shaping the way we view the challenge of treating injury and disease. In particular, emerging biofabrication techniques that allow the precise construction of complex biological structures have reinvigorated the effort to engineer replacement tissues and organs. In addition to the potential for direct therapeutic approaches, advanced engineered tissues promise to significantly improve in vitro studies of fundamental cell biology and disease processes, and expedite drug development.
Drop-on-demand cell printing technologies are at the forefront of these advances in biofabrication. These approaches offer the ability to place living cells, biomaterials and other factors in defined arrangements in two or three dimensions in order to reproduce the complex spatial interplay that regulates tissue function. Significant progress towards this goal has been made over the last decade, but the design of bioinks remains a key challenge due to the need to simultaneously satisfy disparate engineering and biological requirements. The aim of this thesis was to develop bioinks for drop-on-demand cell printing that enable the robust deposition of living cells. Specifically, a suitable bio-ink should be non-cytotoxic, prevent cell settling and aggregation, possess optimal fluid properties (i.e. viscosity and surface tension) for drop-on-demand printing and contain minimal dry mass.
Bio-inks were developed by forming gellan gum (GG) microgel suspensions in cell culture media by applying shear during gelation. At a low polymer concentration (0.05% w/v) the bio-ink showed a yield stress (~ 43 mPa), while exhibiting a low viscosity (~ 1.7 mPa.s) at high shear rates (103 s-1). These properties were shown to prevent cell settling and aggregation without affecting printability. Surfactants were added to the formulation to achieve surface tension reduction for inkjet printing. Addition of the fluorosurfactant, Novec FC-4430, allowed a suitable surface tension (~ 30 mN/m at 0.05% v/v) to be achieved, while Poloxamer 188 (P188) was included (0.1% v/v) for its reported cell-protecting qualities. Neither surfactant significantly affected the bio-ink structure or rheology, and C2C12 (skeletal muscle) and PC12 (pheocromocytoma) cells exposed to the surfactant-containing bio-ink for 2 hr exhibited normal viability, proliferation and differentiation.
The bio-ink formulations, with and without surfactants, proved suitable for cell deposition by microvalve and inkjet printing, respectively. The bio-ink enabled reproducible cell output over 1 hr printing periods from both a microvalve printer (Deerac Equator GX1) and multiple-nozzle piezoelectric inkjet print heads (Xaar-126). Printed cells exhibited phenotypic responses that were comparable to controls. It was also demonstrated that P188 had a protective effect on cells during inkjet printing.
Inkjet cell printing using the bio-ink was applied to the fabrication of two dimensional…
Subjects/Keywords: IPRI; Biofabrication; cell printing; hydrogel; tisue engineering
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APA (6th Edition):
Ferris, C. (2013). Bio-inks for drop-on-demand cell printing. (Doctoral Dissertation). University of Wollongong. Retrieved from 090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875
Chicago Manual of Style (16th Edition):
Ferris, Cameron. “Bio-inks for drop-on-demand cell printing.” 2013. Doctoral Dissertation, University of Wollongong. Accessed January 24, 2021.
090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875.
MLA Handbook (7th Edition):
Ferris, Cameron. “Bio-inks for drop-on-demand cell printing.” 2013. Web. 24 Jan 2021.
Vancouver:
Ferris C. Bio-inks for drop-on-demand cell printing. [Internet] [Doctoral dissertation]. University of Wollongong; 2013. [cited 2021 Jan 24].
Available from: 090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875.
Council of Science Editors:
Ferris C. Bio-inks for drop-on-demand cell printing. [Doctoral Dissertation]. University of Wollongong; 2013. Available from: 090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875
University of Wollongong
5.
Stevens, Leo Robert.
Materials and processes for the biofabrication of peripheral nerve guides.
Degree: PhD, 2016, University of Wollongong
URL: 090301
Biomaterials,
100404
Regenerative
Medicine
(incl.
Stem
Cells
and
Tissue
Engineering)
;
https://ro.uow.edu.au/theses/4955
► Injuries sustained to the peripheral nervous system disrupt the body’s major signalling pathway, leading to pain or paralysis. The treatment of serious peripheral nerve…
(more)
▼ Injuries sustained to the peripheral nervous system disrupt the body’s major signalling pathway, leading to pain or paralysis. The treatment of serious peripheral nerve injuries currently relies on the use of autografts, whereby secondary nerves are sacrificed to treat the pathology. Recently, scientists and clinicians have sought alternative treatments based on engineered tissue scaffolds that may effectively replace grafted tissues. Several peripheral nerve guides (PNGs) are already available, however patient recovery outcomes using PNGs have thus far been poor compared to autograft treatments. The limited efficacy of current-generation PNGs has been attributed to their failure to replicate the complex structure and biofunctionality of nerve tissue. In this thesis, we explore materials and fabrication processes with the aim of forming multifunctionalised PNG fibers with the potential to accelerate nerve repair.
The anionic polysaccharide gellan gum (GG) was assessed for its potential to directly encapsulate neural cells, as well as contribute to the PNG fabrication process. GG was purified to its sodium salt (NaGG), which exhibited processing characteristics that were favourable for biofabrication techniques including casting, reactive printing and wet spinning. NaGG hydrogels were visualised using scanning electron microscopy, confirming high levels of internal porosity. A variety of cell types including fibroblasts, skeletal muscle cells and neurons were encapsulated within NaGG hydrogels and remained highly viable. However, many cells also exhibited increased clustering and diminished differentiation compared to controls, with a reduction in neuronal differentiation and neurite extension being of most concern for PNG applications. Mechanical testing of NaGG hydrogels revealed them to be weaker and more rigid than neural tissues, in line with comparable polysaccharide hydrogels. An alternative material, type-I collagen, was successfully extracted from rat tails at high purity and confirmed to be highly supportive for PC12 cell differentiation. Electron micrographs of our type-I collagen hydrogels revealed a porous fibrillar network in line with previous reports. Whilst collagen’s gelation behaviour was not ideal, rheological studies identified a short processing window that was later applied for the wet spinning of multi-material fibers. Finally, a short peptide sequence containing the cell-adhesion motif arginine-glycineaspartate (RGD) was coupled to NaGG using carbodiimide chemistry to 40 % yield. RGD-GG retained the favourable processing characteristics of NaGG, whilst improving metabolic activity in encapsulated cells and supporting high rates of differentiation in encapsulated skeletal muscle cells and primary cortical neurons. However PC12 cells remained poorly differentiated in RGD-GG and it was concluded that the PNG should have a multi-material design incorporating distinct materials tailored for cell support, mechanical strength and processing characteristics.
Conductive materials were…
Subjects/Keywords: tissue engineering; biomaterials; biofabrication; nerve regeneration; IPRI
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APA (6th Edition):
Stevens, L. R. (2016). Materials and processes for the biofabrication of peripheral nerve guides. (Doctoral Dissertation). University of Wollongong. Retrieved from 090301 Biomaterials, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/4955
Chicago Manual of Style (16th Edition):
Stevens, Leo Robert. “Materials and processes for the biofabrication of peripheral nerve guides.” 2016. Doctoral Dissertation, University of Wollongong. Accessed January 24, 2021.
090301 Biomaterials, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/4955.
MLA Handbook (7th Edition):
Stevens, Leo Robert. “Materials and processes for the biofabrication of peripheral nerve guides.” 2016. Web. 24 Jan 2021.
Vancouver:
Stevens LR. Materials and processes for the biofabrication of peripheral nerve guides. [Internet] [Doctoral dissertation]. University of Wollongong; 2016. [cited 2021 Jan 24].
Available from: 090301 Biomaterials, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/4955.
Council of Science Editors:
Stevens LR. Materials and processes for the biofabrication of peripheral nerve guides. [Doctoral Dissertation]. University of Wollongong; 2016. Available from: 090301 Biomaterials, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/4955
University of South Carolina
6.
Rodriguez-Rivera, Veronica.
Novel Biofabrication Technologies to Recapitulate <i>In Vivo</i> Geometries in Collagen Hydrogels.
Degree: PhD, Chemical Engineering, 2014, University of South Carolina
URL: https://scholarcommons.sc.edu/etd/2998
► Tissue engineering and regenerative medicine aims to restore form and function to tissues that have been lost or damaged due to disease, congenital defect,…
(more)
▼ Tissue engineering and regenerative medicine aims to restore form and function to tissues that have been lost or damaged due to disease, congenital defect, or trauma. Biomaterials suitable to restore these complex tissues need to provide a balance between chemical and mechanical properties, providing accurate cell-matrix interaction and induce in vivo behaviors such as proliferation, differentiation and migration. It also requires that physical and chemical cues be presented to the body in the proper temporal and spatial pattern. The extracellular matrix exerts forces that are transmitted through focal adhesion causing changes in the cell behavior. The hypothesis for this dissertation work was that by using the ideal substrate composition, the geometrical features and the proper elasticity of the substrate, we can recapitulate the microenviroments of the in vivo niche and control cell behavior. The self renewal capabilities of muscle stem cells, satellite cells, are lost once they are culture in a rigid environment where they commit to become skeletal myocytes. In our studies, we tuned the elasticity of a collagen hydrogel to their in vivo elastic modulus and we maintain the quiescence phenotype. We developed reaction electrospinning, which is a technique that combines two processes: electrospinning and fibrillogenesis. For the first time in literature, we show that as we spin collagen monomers and microfibrils using benign solvents, they undergo fibrillogenesis resulting in fibrous collagen scaffolds. Also, we developed a sacrificial material, BSA rubber, which can deliver specific geometrical templates to a collagen material, recapitulating the internal three-dimensional architectures. Our prototype consist of a 3D branched architecture using type I collagen. Overall, we developed fabrication techniques that allow us to tune the elasticity of the matrix, create fibrous scaffolds, and incorporate the geometrical features into an in vitro collagen scaffold. These techniques combine state of the art imaging, micromachining and selective enzymatic activity to create three dimensional biomaterials. The overall goal of this work is to fabricate custom made tissue scaffolds that replicate in vivo tissue composition, architecture, and cell population for broad application in tissue engineering. These new biomaterials will enable the modulation of cell potential, and thus, accelerate discovery in the field of regenerative medicine.
Advisors/Committee Members: John W. Weidner, Michael J. Yost.
Subjects/Keywords: Chemical Engineering; Engineering; Biofabrication; Collagen; Tissue Engineering
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APA (6th Edition):
Rodriguez-Rivera, V. (2014). Novel Biofabrication Technologies to Recapitulate <i>In Vivo</i> Geometries in Collagen Hydrogels. (Doctoral Dissertation). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/2998
Chicago Manual of Style (16th Edition):
Rodriguez-Rivera, Veronica. “Novel Biofabrication Technologies to Recapitulate <i>In Vivo</i> Geometries in Collagen Hydrogels.” 2014. Doctoral Dissertation, University of South Carolina. Accessed January 24, 2021.
https://scholarcommons.sc.edu/etd/2998.
MLA Handbook (7th Edition):
Rodriguez-Rivera, Veronica. “Novel Biofabrication Technologies to Recapitulate <i>In Vivo</i> Geometries in Collagen Hydrogels.” 2014. Web. 24 Jan 2021.
Vancouver:
Rodriguez-Rivera V. Novel Biofabrication Technologies to Recapitulate <i>In Vivo</i> Geometries in Collagen Hydrogels. [Internet] [Doctoral dissertation]. University of South Carolina; 2014. [cited 2021 Jan 24].
Available from: https://scholarcommons.sc.edu/etd/2998.
Council of Science Editors:
Rodriguez-Rivera V. Novel Biofabrication Technologies to Recapitulate <i>In Vivo</i> Geometries in Collagen Hydrogels. [Doctoral Dissertation]. University of South Carolina; 2014. Available from: https://scholarcommons.sc.edu/etd/2998
Tulane University
7.
Vinson, Benjamin.
CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems.
Degree: 2020, Tulane University
URL: https://digitallibrary.tulane.edu/islandora/object/tulane:120508
Subjects/Keywords: Microphysiological systems; Biofabrication; Cell Culture
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APA (6th Edition):
Vinson, B. (2020). CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:120508
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vinson, Benjamin. “CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems.” 2020. Thesis, Tulane University. Accessed January 24, 2021.
https://digitallibrary.tulane.edu/islandora/object/tulane:120508.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vinson, Benjamin. “CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems.” 2020. Web. 24 Jan 2021.
Vancouver:
Vinson B. CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems. [Internet] [Thesis]. Tulane University; 2020. [cited 2021 Jan 24].
Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:120508.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vinson B. CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems. [Thesis]. Tulane University; 2020. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:120508
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
8.
Visser, J.
Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.
Degree: 2015, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/318088
► Implants were biofabricated for the repair of chondral and osteochondral articular joint defects. The implants were based on gelatin methacrylamide (GelMA) hydrogels combined with printed…
(more)
▼ Implants were biofabricated for the repair of chondral and osteochondral articular joint defects. The implants were based on gelatin methacrylamide (GelMA) hydrogels combined with printed fibers from polycaprolactone (PCL) for mechanical reinforcement. In Part I of the thesis, biological modifications of GelMA by the addition of matrix derived from cartilage, meniscus or tendon tissue did not show a positive effect on in vitro cartilage matrix production by encapsulated chondrocytes or MSCs. Furthermore, MSCs in GelMA in a subcutaneous rat model could not be locked in their chondrogenic state, considering the evident process of endochondral bone formation in these constructs. Both the quantity and quality of bone formed by MSCs in GelMA are nonetheless encouraging for bone tissue engineers. In Part II, hydrogels were successfully reinforced with PCL microfibers. These hydrogel/microfiber composites approached the stiffness and elasticity of articular cartilage and permitted the formation of cartilage matrix by embedded chondrocytes. The repair of focal cartilage defects with reinforced GelMA gels is currently evaluated in eight Shetland ponies, with a follow-up of one year, including several arthroscopic imaging techniques and standardized gait analysis. In Part III, reinforced GelMA was applied for the
biofabrication of implants for the restoration of large joint defects. Anatomically-shaped, osteochondral implants were fabricated and further developed for the restoration of the complete shoulder joint in rabbits. A pilot study on the strength and fixation of the implants in ongoing in three rabbits. Altogether, in this thesis, biologically active implants have been biofabricated with an imposed internal organization and external architecture. The regeneration of bone and cartilage tissue in these constructs was demonstrated in vitro and in ectopic locations in rodents. Large animal models were established and are currently conducted to evaluate the potential of these implants for the repair of focal or complete articular joint defects.
Advisors/Committee Members: Dhert, W.J.A., Malda, J., Gawlitta, D..
Subjects/Keywords: biofabrication; 3D-printing; Bone; cartilage; tissue-engineering; hydrogel; implant; biomaterials; orthopedics
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Visser, J. (2015). Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/318088
Chicago Manual of Style (16th Edition):
Visser, J. “Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.” 2015. Doctoral Dissertation, Universiteit Utrecht. Accessed January 24, 2021.
http://dspace.library.uu.nl:8080/handle/1874/318088.
MLA Handbook (7th Edition):
Visser, J. “Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.” 2015. Web. 24 Jan 2021.
Vancouver:
Visser J. Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2015. [cited 2021 Jan 24].
Available from: http://dspace.library.uu.nl:8080/handle/1874/318088.
Council of Science Editors:
Visser J. Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. [Doctoral Dissertation]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/318088
University of Wollongong
9.
Zhang, Binbin.
Fabrication of drug delivery systems.
Degree: Doctor of
Philosophy, 2014, University of Wollongong
URL: ;
https://ro.uow.edu.au/theses/4326
► The aim of the studies in this thesis was to investigate drug delivery systems using a variety of fabrication techniques that can be used…
(more)
▼ The aim of the studies
in this thesis was to investigate drug delivery systems
using a variety of fabrication techniques that can be
used for biomedical devices. This thesis was conducted in
partnership with the HEARing Cooperative Research Centre
(CRC) with the aim of developing drug delivery systems
that can be incorporated into the cochlear implant
(Cochlear Pty. Ltd is a partner of the HEARing CRC).
Therefore the materials used to form the drug delivery
systems and the fabrication approaches were chosen and
used with the final application of the cochlear implant
in mind. First, a multifunctional
polypyrrole based system was developed to
electrochemically deliver dexamethasone disodium
phosphate (DexP) - a derivative of anti-inflammatory drug
dexamethasone (Dex). Additionally this system was
modified to render the surface resistance to nonspecific
protein adsorption. This study investigated different
conditions for the electropolymerisation of DexP doped
polypyrrole (PPy/DexP) films, and their impacts on the
film properties (e.g. morphology, impedance) and the
electrically-stimulated drug release profiles.
Poly(ethylene glycol) methyl ether thiol (PEG-SH) with
different molecular weights were introduced to modify the
PPy/DexP film surface and a quartz crystal microbalance
(QCM) was used to study the modification of the PPy/DexP
films, as well as protein interaction with PPy/DexP films
before and after the PEG-SH modification.
The second part of this study involved exploring
the possibility to increase the loading of the
anti-inflammatory drug using a hydrogel reservoir system.
A multipleprinthead inkjet printer was developed to print
alginate-chitosan hydrogels. The ink formulations were
investigated and optimised to achieve optimum printing
with alginate or chitosan, as well as the reactive
printing of alginate-chitosan hydrogel with both inks.
Phenol red or DexP was loaded in the alginate ink and
incorporated into the printed alginate-chitosan hydrogel
samples during the reactive printing, and their in vitro
release profiles were studied. In the
third part of this study, the application of melt
extrusion printing to fabricate three-dimensional (3D)
scaffolds for drug delivery was demonstrated.
Polycaprolactone (PCL) was chosen for its good
biocompatibility and processability. PCL-Dex material was
made by mixing Dex within PCL at varying concentrations
using a solvent casting method. The processability of
PCL-Dex was investigated through a series of
characterisation methods, including thermal gravimetric
…
Subjects/Keywords: Drug delivery; fabrication; biofabrication; 3D printing; conducting polymers; biomaterials
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APA (6th Edition):
Zhang, B. (2014). Fabrication of drug delivery systems. (Doctoral Dissertation). University of Wollongong. Retrieved from ; https://ro.uow.edu.au/theses/4326
Chicago Manual of Style (16th Edition):
Zhang, Binbin. “Fabrication of drug delivery systems.” 2014. Doctoral Dissertation, University of Wollongong. Accessed January 24, 2021.
; https://ro.uow.edu.au/theses/4326.
MLA Handbook (7th Edition):
Zhang, Binbin. “Fabrication of drug delivery systems.” 2014. Web. 24 Jan 2021.
Vancouver:
Zhang B. Fabrication of drug delivery systems. [Internet] [Doctoral dissertation]. University of Wollongong; 2014. [cited 2021 Jan 24].
Available from: ; https://ro.uow.edu.au/theses/4326.
Council of Science Editors:
Zhang B. Fabrication of drug delivery systems. [Doctoral Dissertation]. University of Wollongong; 2014. Available from: ; https://ro.uow.edu.au/theses/4326
University of Cambridge
10.
Li, Zhaoying.
Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues.
Degree: PhD, 2017, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/270349
► Recreating organ-specific microenvironments of the extracellular matrix (ECM) in vitro has been an ongoing challenge in biofabrication. In this study, I present a biofunctional ECM-mimicking…
(more)
▼ Recreating organ-specific microenvironments of the extracellular matrix (ECM) in vitro has been an ongoing challenge in biofabrication. In this study, I present a biofunctional ECM-mimicking protein scaffold with tunable biochemical, mechanical and topographical properties. This scaffold, formed by microfibres, displays three favorable characteristics as a cell culture platform: high-loading of key ECM proteins, single-layered mesh membrane with controllable mesh size, and flexibility for supporting a range of cell culture configurations. Decellularized extracellular matrix (dECM) powder was used to fabricate this protein scaffold, as a close replicate of the chemical composition of physiological ECM. The highest dECM concentration in the solidified protein scaffold was 50 wt%, with gelatin consisting the rest. In practice, a high density of dECM-laden nano- to microfibres was directly patterned on a variety of substrates to form a single layer of mesh membrane, using the low-voltage electrospinning patterning (LEP) method. The smallest fibre diameter was measured at 450 nm, the smallest mesh size of the membrane was below 1 μm, and the thickness of the membrane was estimated to be less than 2 μm. This fabrication method demonstrated a good preservation of the key ECM proteins and growth factors, including collagen IV, laminin, fibronectin, VEGF and b-FGF. The integrated fibrous mesh exhibited robust mechanical properties, with tunable fibril Young’s modulus for over two orders of magnitude in the physiological range (depending on the dECM concentration). Combining this mesh membrane with 3D printing, a cell culture device was constructed. Co-culture of human glomerulus endothelial cells and podocytes was performed on this device, to simulate the blood-to-urine interface in vitro. Good cell attachment and viability were demonstrated, and specific cell differentiation and fibronectin secretion were observed. This dECM-laden protein scaffold sees the potential to be incorporated into a glomerulus-on-chip model, to further improve the physiological relevance of in vitro pathological models.
Subjects/Keywords: Decellularized extracellular matrix; Biofabrication; Microfibre; Protein scaffold; Electrospinning; Glomerulus; Kidney; Bioengineering
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APA ·
Chicago ·
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Manager
APA (6th Edition):
Li, Z. (2017). Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/270349
Chicago Manual of Style (16th Edition):
Li, Zhaoying. “Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues.” 2017. Doctoral Dissertation, University of Cambridge. Accessed January 24, 2021.
https://www.repository.cam.ac.uk/handle/1810/270349.
MLA Handbook (7th Edition):
Li, Zhaoying. “Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues.” 2017. Web. 24 Jan 2021.
Vancouver:
Li Z. Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues. [Internet] [Doctoral dissertation]. University of Cambridge; 2017. [cited 2021 Jan 24].
Available from: https://www.repository.cam.ac.uk/handle/1810/270349.
Council of Science Editors:
Li Z. Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues. [Doctoral Dissertation]. University of Cambridge; 2017. Available from: https://www.repository.cam.ac.uk/handle/1810/270349
University of Washington
11.
Swift, Brian James Fullerton.
Engineering Solid Binding Proteins for the Biofabrication of Environmentally Friendly Multi-functional Quantum Dots.
Degree: PhD, 2016, University of Washington
URL: http://hdl.handle.net/1773/35548
► Methods that emulate Nature’s remarkable ability to synthesize chemically and structurally intricate architectures are of considerable interest for the fabrication of industrially-relevant materials and systems.…
(more)
▼ Methods that emulate Nature’s remarkable ability to synthesize chemically and structurally intricate architectures are of considerable interest for the fabrication of industrially-relevant materials and systems. In this work, we design and engineer solid-binding proteins that drive the formation and assembly of multi-functional nanomaterials. We first describe a method to biomineralize Mn-doped ZnS immuno-quantum dots with a minimized protein and study how such protein-coated nanocrystals are taken up by, and impact the physiology of the model bacterium Escherichia coli. We next design and build ZnS-binding derivatives of Green Fluorescent Protein to mineralize nanocrystals that combine inorganic and organic fluorescence properties and combine these with DNA aptamers and silica phases to create small-molecule sensors. Finally, we demonstrate that solid-binding proteins can be used to solubilize carbon nanotubes and to selectively label their ends. Collectively, our results emphasize the power of protein engineering for the environmentally friendly synthesis of functional nanomaterials.
Advisors/Committee Members: Baneyx, Francois (advisor).
Subjects/Keywords: Biofabrication; Protein engineering; Quantum dots; Chemical engineering; chemical engineering
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APA (6th Edition):
Swift, B. J. F. (2016). Engineering Solid Binding Proteins for the Biofabrication of Environmentally Friendly Multi-functional Quantum Dots. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/35548
Chicago Manual of Style (16th Edition):
Swift, Brian James Fullerton. “Engineering Solid Binding Proteins for the Biofabrication of Environmentally Friendly Multi-functional Quantum Dots.” 2016. Doctoral Dissertation, University of Washington. Accessed January 24, 2021.
http://hdl.handle.net/1773/35548.
MLA Handbook (7th Edition):
Swift, Brian James Fullerton. “Engineering Solid Binding Proteins for the Biofabrication of Environmentally Friendly Multi-functional Quantum Dots.” 2016. Web. 24 Jan 2021.
Vancouver:
Swift BJF. Engineering Solid Binding Proteins for the Biofabrication of Environmentally Friendly Multi-functional Quantum Dots. [Internet] [Doctoral dissertation]. University of Washington; 2016. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/1773/35548.
Council of Science Editors:
Swift BJF. Engineering Solid Binding Proteins for the Biofabrication of Environmentally Friendly Multi-functional Quantum Dots. [Doctoral Dissertation]. University of Washington; 2016. Available from: http://hdl.handle.net/1773/35548
University of South Carolina
12.
Laughlin, Michael Richard.
A Survey of the Kinetic Monte Carlo Algorithm as Applied to a Multicellular System.
Degree: MA, Mathematics, 2015, University of South Carolina
URL: https://scholarcommons.sc.edu/etd/3275
► We explore the origins and implementation of the Kinetic Monte Carlo method on a system of cells suspended in a liquid media. The situation…
(more)
▼ We explore the origins and implementation of the Kinetic Monte Carlo method on a system of cells suspended in a liquid media. The situation presented herein has applications in the emerging field of
biofabrication, which may have lasting impacts in medical science. The theory behind the method is explained in detail, starting with its emergence in the 1960s, and two major improvements to the scaling of the method are presented, along with a restriction to a special case. Finally, we give the results of several simulations.
Advisors/Committee Members: Yi Sun.
Subjects/Keywords: Mathematics; Physical Sciences and Mathematics; Biofabrication; Bioprinting; Kinetic Monte Carlo
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APA (6th Edition):
Laughlin, M. R. (2015). A Survey of the Kinetic Monte Carlo Algorithm as Applied to a Multicellular System. (Masters Thesis). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/3275
Chicago Manual of Style (16th Edition):
Laughlin, Michael Richard. “A Survey of the Kinetic Monte Carlo Algorithm as Applied to a Multicellular System.” 2015. Masters Thesis, University of South Carolina. Accessed January 24, 2021.
https://scholarcommons.sc.edu/etd/3275.
MLA Handbook (7th Edition):
Laughlin, Michael Richard. “A Survey of the Kinetic Monte Carlo Algorithm as Applied to a Multicellular System.” 2015. Web. 24 Jan 2021.
Vancouver:
Laughlin MR. A Survey of the Kinetic Monte Carlo Algorithm as Applied to a Multicellular System. [Internet] [Masters thesis]. University of South Carolina; 2015. [cited 2021 Jan 24].
Available from: https://scholarcommons.sc.edu/etd/3275.
Council of Science Editors:
Laughlin MR. A Survey of the Kinetic Monte Carlo Algorithm as Applied to a Multicellular System. [Masters Thesis]. University of South Carolina; 2015. Available from: https://scholarcommons.sc.edu/etd/3275
University of Maryland
13.
Gordonov, Tanya.
Bridging the biology-electronics communication gap with redox signaling.
Degree: Bioengineering, 2015, University of Maryland
URL: http://hdl.handle.net/1903/17096
► Electronic and biological systems both have the ability to sense, respond to, and communicate relevant data. This dissertation aims to facilitate communication between the two…
(more)
▼ Electronic and biological systems both have the ability to sense, respond to, and communicate relevant data. This dissertation aims to facilitate communication between the two and create bio-hybrid devices that can process the breadths of both electronic and biological information. We describe the development of novel methods that bridge this bi-directional communication gap through the use of electronically and biologically relevant redox molecules for controlled and quantitative information transfer. Additionally, we demonstrate that the incorporation of biological components onto microelectronic systems can open doors for improved capabilities in a variety of fields.
First, we describe the original use of redox molecules to electronically control the activity of an enzyme on a chip. Using
biofabrication techniques, we assembled HLPT, a fusion protein which generates the quorum sensing molecule autoinducer-2, on an electrodeposited chitosan film on top of an electrode. This allows the electrode to controllably oxidize the enzyme in situ through a redox mediator, acetosyringone. We successfully showed that activity decrease and bacterial quorum sensing response are proportional to the input charge.
To engineer bio-electronic communication with cells, we first aimed for better characterizing an electronic method for measuring cell response. We engineered Escherichia coli (E.coli) cells to respond to autoinducer-2 by producing the β-galactosidase enzyme. We then investigated an existing electrochemical method for detecting β-galactosidase activity by measuring a redox-active product of the cleavage of the added substrate molecule PAPG. In our novel findings, the product, PAP, was found to be produced at a rate that correlated with the standard spectrophotometric method for measuring β-galactosidase, the Miller assay, in both whole live and lysed cells. Conversely, to translate electronic signals to something cells can understand, we used pyocyanin, a redox drug which oxidizes the E.coli SoxR protein and allows transcription from the soxS promoter. We utilized electronic control of ferricyanide, an electron acceptor, to amplify the production of a reporter from soxS. With this novel method, we show that production of reporter depends on the frequency and amplitude of electronic signals, and investigate the method’s metabolic effects. Overall, the work in this dissertation makes strides towards the greater goal of creating multi-functional bio-hybrid devices.
Advisors/Committee Members: Bentley, William E (advisor).
Subjects/Keywords: Molecular biology; Nanotechnology; biochip; biofabrication; bionanotechnology; biosensor; electrochemistry; synthetic biology
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APA ·
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MLA ·
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CSE |
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Manager
APA (6th Edition):
Gordonov, T. (2015). Bridging the biology-electronics communication gap with redox signaling. (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/17096
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gordonov, Tanya. “Bridging the biology-electronics communication gap with redox signaling.” 2015. Thesis, University of Maryland. Accessed January 24, 2021.
http://hdl.handle.net/1903/17096.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gordonov, Tanya. “Bridging the biology-electronics communication gap with redox signaling.” 2015. Web. 24 Jan 2021.
Vancouver:
Gordonov T. Bridging the biology-electronics communication gap with redox signaling. [Internet] [Thesis]. University of Maryland; 2015. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/1903/17096.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gordonov T. Bridging the biology-electronics communication gap with redox signaling. [Thesis]. University of Maryland; 2015. Available from: http://hdl.handle.net/1903/17096
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Delft University of Technology
14.
Zhou, Jiwei (author).
Interwoven: Designing Biodigital Objects with Plant Roots: Exploring Material Structure and Experience.
Degree: 2019, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:ffdcf947-06df-4941-a587-bdf008f87783
► Interwoven is a textile grown from plant roots, showing the intelligence of plants. It is originally from the attempt of training plant roots to form…
(more)
▼ Interwoven is a textile grown from plant roots, showing the intelligence of plants. It is originally from the attempt of training plant roots to form a manmade pattern since 2015 by visual artist Diana Scherer based in Amsterdam. Due to fragility, it still remains an artistic work. However, Interwoven has a great potential for sustainable product design if further development is made through altering the material structure. Collaborating with Materials Experience Lab of Industrial Design Engineering in TU Delft, Diana Scherer wants to bring an artistic material closer to people’s daily life. The author, by following the Material Driven Design method (Karana et al., 2015)*, develop the material by altering material structure through hacking the growing process, incorporating generative design techniques and the results from the user studies, with a particular emphasis on people’s experience on interpretive level. Digital Fabrication techniques are used to design the structural pattern for achieving functionally graded material properties (e.g. spatially graded stiffness), and shape optimisation. Based on Diana Scherer’s experience and early experiments, a few techniques are synthesized and developed in the tinkering with Interwoven. Some potential structures for digital biofabrication are: [1] root growth can be manipulated to mirror digitally generated patterns, which would provide intended technical and experiential characteristics in Interwoven; [2] roots grown in agar gel change properties to stiffer and stronger by hand feeling (further mechanical tests are needed); [3] roots can sew through descrete obstacles in their growing direction and these obstacles can be designed with digital fabrication techniques. Combining the insights and experiential studies, a material concept has been created: showing roots glue-ability to porous materials and growing traces to blend nature and man-made world. The material experience vision is to exhibit the glue-ability of Interwoven through a daily object co-created by roots and digital manufactured structures and bring forward the collision and collaboration between natural growth and man-made world. The final product concept is to imitate one of the most mass-produced daily products - IKEA ALSEDA, questioning the current way of manufacturing and material use. The co-creation structure increased durability of Interwoven.
Interwoven
Design for Interaction
Advisors/Committee Members: Karana, Elvin (mentor), Wu, Jun (mentor), Delft University of Technology (degree granting institution).
Subjects/Keywords: Material Driven Design; Biomaterials; Digital Biofabrication; Growing Material
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APA (6th Edition):
Zhou, J. (. (2019). Interwoven: Designing Biodigital Objects with Plant Roots: Exploring Material Structure and Experience. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:ffdcf947-06df-4941-a587-bdf008f87783
Chicago Manual of Style (16th Edition):
Zhou, Jiwei (author). “Interwoven: Designing Biodigital Objects with Plant Roots: Exploring Material Structure and Experience.” 2019. Masters Thesis, Delft University of Technology. Accessed January 24, 2021.
http://resolver.tudelft.nl/uuid:ffdcf947-06df-4941-a587-bdf008f87783.
MLA Handbook (7th Edition):
Zhou, Jiwei (author). “Interwoven: Designing Biodigital Objects with Plant Roots: Exploring Material Structure and Experience.” 2019. Web. 24 Jan 2021.
Vancouver:
Zhou J(. Interwoven: Designing Biodigital Objects with Plant Roots: Exploring Material Structure and Experience. [Internet] [Masters thesis]. Delft University of Technology; 2019. [cited 2021 Jan 24].
Available from: http://resolver.tudelft.nl/uuid:ffdcf947-06df-4941-a587-bdf008f87783.
Council of Science Editors:
Zhou J(. Interwoven: Designing Biodigital Objects with Plant Roots: Exploring Material Structure and Experience. [Masters Thesis]. Delft University of Technology; 2019. Available from: http://resolver.tudelft.nl/uuid:ffdcf947-06df-4941-a587-bdf008f87783
15.
Faria Bellani, Caroline.
Electrospun biocomposites and 3D microfabrication for bone tissue enginneering : Biocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux.
Degree: Docteur es, Chimie des polymères - Chimie des matériaux, 2018, Strasbourg; Universidade de São Paulo (Brésil)
URL: http://www.theses.fr/2018STRAE028
► Des membranes biodégradables en polycaprolactone pour la régénération osseuse guidée, obtenues par electrospinning, incorporés avec différents rapports de nanocomposites de nanocristaux de cellulose et du…
(more)
▼ Des membranes biodégradables en polycaprolactone pour la régénération osseuse guidée, obtenues par electrospinning, incorporés avec différents rapports de nanocomposites de nanocristaux de cellulose et du Biosilicate®, ont été fabriquées, avec propriétés mécaniques et ostéogéniques améliorés. En tant que stratégie de vascularisation rapide, un greffon biomimétique suturable obtenue par fusion de membranes électrofilées a été fabriqué, avec des motifs poreux obtenus par micro- usinage au laser pour permettre la migration des cellules endothéliales vers le greffon osseux. Les motifs poreux créés sur les greffes suturables ont permis aux cellules endothéliales migrer vers la culture 3D des ostéoblastes dans des hydrogels en gélatine méthacryloyl (GelMA), et des structures 3D ont été observées. Par conséquent, cette stratégie peut être utilisée pour améliorer la taille et la survie des implants osseux biofabriqués, en accélérant la traduction clinique de l'ingénierie du tissu osseux.
Biodegradable membranes for guided bone regeneration, made of polycaprolactone, obtained by electrospinning, incorporated with different nanocomposite ratios of cellulose nanocrystals and Biosilicate®, have been manufactured, with improved mechanical and osteogenic properties. As fast vascularization strategy, a suturable biomimetic graft obtained by fusion of electrospun membranes was fabricated, with porous patterns obtained by laser micromachining to allow migration of endothelial cells to the bone graft. The porous patterns created on the suturable grafts allowed the endothelial cells to migrate to the 3D culture of the osteoblasts in gelatin methacryloyl (GelMA), and 3D structures were observed. Therefore, this strategy can be used to improve the size and survival of biofabricated bone implants, accelerating the clinical translation of bone tissue engineering.
Advisors/Committee Members: Schlatter, Guy (thesis director), Minarelli Gaspar, Ana Maria (thesis director), Branciforti, Márcia Cristina (thesis director).
Subjects/Keywords: Ingénierie tissulaire; Os; Biocomposites; Electrospinning; Biofabrication; Vascularisation; Tissue Engineering; Bone; Biocomposites; Electrospinning; Biofabrication; Vascularization; Engenharia tecidual; Osso; Biocompósitos; Electrofiaçăo; Biofabricaçăo; Vascularizaçăo; 547.8; 610.28; 617.95
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APA (6th Edition):
Faria Bellani, C. (2018). Electrospun biocomposites and 3D microfabrication for bone tissue enginneering : Biocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux. (Doctoral Dissertation). Strasbourg; Universidade de São Paulo (Brésil). Retrieved from http://www.theses.fr/2018STRAE028
Chicago Manual of Style (16th Edition):
Faria Bellani, Caroline. “Electrospun biocomposites and 3D microfabrication for bone tissue enginneering : Biocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux.” 2018. Doctoral Dissertation, Strasbourg; Universidade de São Paulo (Brésil). Accessed January 24, 2021.
http://www.theses.fr/2018STRAE028.
MLA Handbook (7th Edition):
Faria Bellani, Caroline. “Electrospun biocomposites and 3D microfabrication for bone tissue enginneering : Biocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux.” 2018. Web. 24 Jan 2021.
Vancouver:
Faria Bellani C. Electrospun biocomposites and 3D microfabrication for bone tissue enginneering : Biocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux. [Internet] [Doctoral dissertation]. Strasbourg; Universidade de São Paulo (Brésil); 2018. [cited 2021 Jan 24].
Available from: http://www.theses.fr/2018STRAE028.
Council of Science Editors:
Faria Bellani C. Electrospun biocomposites and 3D microfabrication for bone tissue enginneering : Biocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux. [Doctoral Dissertation]. Strasbourg; Universidade de São Paulo (Brésil); 2018. Available from: http://www.theses.fr/2018STRAE028
16.
Guduric, Vera.
3D Printing and Characterization of PLA Scaffolds for Layer-by-Layer BioAssembly in Tissue Engineering : Impression 3D et Caractérisation des Scaffolds en PLA pour Assemblage Couche par Couche en Ingénierie Tissulaire.
Degree: Docteur es, Biologie cellulaire et physiopathologie, 2017, Bordeaux
URL: http://www.theses.fr/2017BORD0858
► L’Ingénierie tissulaire (IT) est un domaine interdisciplinaire qui applique les principes de l'ingénierie et des sciences de la vie au développement de substituts biologiques afin…
(more)
▼ L’Ingénierie tissulaire (IT) est un domaine interdisciplinaire qui applique les principes de l'ingénierie et des sciences de la vie au développement de substituts biologiques afin de restaurer, maintenir ou améliorer la fonction tissulaire. Sa première application consiste à remplacer les tissus endommagés par des produits cellulaires artificiels. Une autre application de l’IT est basée sur la production des modèles en 2 et 3 dimensions (2D et 3D) pour des études biologiques et pharmacologiques in vitro. Ces modèles ou remplacements de tissus peuvent être fabriqués en utilisant des différentes méthodes de médecine, biologie, chimie, physique, informatique et mécanique, fournissant un micro-environnement spécifique avec différents types de cellules, facteurs de croissance et matrice. L'un des principaux défis de l'IT la pénétration cellulaire limitée dans les parties internes des biomatériaux poreux. Une faible viabilité cellulaire au centre du produit d'IT est la conséquence de la diffusion limitée d'oxygène et de nutriments du fait d’un réseau vasculaire insuffisant dans l'ensemble de la construction 3D. Le BioAssembage couche-par-couche est une nouvelle approche basée sur l'assemblage de petites constructions cellularisées permettant une distribution cellulaire homogène et une vascularisation plus efficace dans des produits d’IT.Notre hypothèse est que l'approche couche-par-couche est plus adaptée à la régénération osseuse que l'approche conventionnelle de l'IT. L'objectif principal de cette thèse était d'évaluer les avantages de l'approche couche-par-couche en utilisant des membranes de polymères imprimées en 3D et ensemencées avec des cellules primaires humaines. Nous avons évalué l'efficacité de la formation du réseau vasculaire in vivo dans toute la construction 3D en utilisant cette approche et en la comparant à l'approche conventionnelle basée sur l'ensemencement des cellules sur la surface des scaffolds massives. Il n'y avait pas de différence significative dans le nombre de vaisseaux sanguins formés en 3D au niveau des parties externes des constructions implantées en site souscutanée chez des souris. Mais dans les parties internes des implants qui n'étaient pas en contact direct avec un tissu hôte, nous avons pu observer une formation des vaisseaux sanguins statistiquement plus efficace lorsque l'approche du bio-assemblage couche-par-couche a été utilisée. Cette formation de réseau vasculaire était plus importante dans le cas de co-cultures que de mono-cultures.Il y avait plusieurs objectifs secondaires dans ce travail. Le premier était de fabriquer des constructions 3D cellularisées pour l'IT en utilisant des membranes d'acide polylactique (PLA) et des cellules primaires humaines : des cellules de stroma de moelle osseuse humaine (HBMSCs) isolées de la moelle osseuse et des cellules progénitrices endothéliales (EPCs) isolées du sang du cordon ombilical. Ensuite, nous avons comparé différentes technologies de fabrication des scaffolds: impression 3D directe à partir de poudre de PLA et impression par fil…
Advisors/Committee Members: Catros, Sylvain (thesis director), Luzanin, Ognjan (thesis director).
Subjects/Keywords: Impression 3D; Acide Poly(lactic); BioAssemblage Couche par Couche; Ingénierie Tissulaire Osseuse; Biofabrication; 3D printing; Poly(lactic) acid; Layer-by-Layer BioAssembly; Bone Tissue Engineering; Biofabrication
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APA (6th Edition):
Guduric, V. (2017). 3D Printing and Characterization of PLA Scaffolds for Layer-by-Layer BioAssembly in Tissue Engineering : Impression 3D et Caractérisation des Scaffolds en PLA pour Assemblage Couche par Couche en Ingénierie Tissulaire. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2017BORD0858
Chicago Manual of Style (16th Edition):
Guduric, Vera. “3D Printing and Characterization of PLA Scaffolds for Layer-by-Layer BioAssembly in Tissue Engineering : Impression 3D et Caractérisation des Scaffolds en PLA pour Assemblage Couche par Couche en Ingénierie Tissulaire.” 2017. Doctoral Dissertation, Bordeaux. Accessed January 24, 2021.
http://www.theses.fr/2017BORD0858.
MLA Handbook (7th Edition):
Guduric, Vera. “3D Printing and Characterization of PLA Scaffolds for Layer-by-Layer BioAssembly in Tissue Engineering : Impression 3D et Caractérisation des Scaffolds en PLA pour Assemblage Couche par Couche en Ingénierie Tissulaire.” 2017. Web. 24 Jan 2021.
Vancouver:
Guduric V. 3D Printing and Characterization of PLA Scaffolds for Layer-by-Layer BioAssembly in Tissue Engineering : Impression 3D et Caractérisation des Scaffolds en PLA pour Assemblage Couche par Couche en Ingénierie Tissulaire. [Internet] [Doctoral dissertation]. Bordeaux; 2017. [cited 2021 Jan 24].
Available from: http://www.theses.fr/2017BORD0858.
Council of Science Editors:
Guduric V. 3D Printing and Characterization of PLA Scaffolds for Layer-by-Layer BioAssembly in Tissue Engineering : Impression 3D et Caractérisation des Scaffolds en PLA pour Assemblage Couche par Couche en Ingénierie Tissulaire. [Doctoral Dissertation]. Bordeaux; 2017. Available from: http://www.theses.fr/2017BORD0858
17.
Guerreiro, Maria Pita.
MYCELIUM MILLENNIUM.
Degree: Industrial Design, 2020, University of Arts
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-7324
► MYCELIUM MILLENNIUM imagines a new era in which biological resources, specifically Fungi and Mycelium, are used to grow a collection of objects for everyday…
(more)
▼ MYCELIUM MILLENNIUM imagines a new era in which biological resources, specifically Fungi and Mycelium, are used to grow a collection of objects for everyday domestic rituals, merging biofabrication and traditional craft. The project is an effort to demystify the transformation of an organism to a biomaterial and at the same time raise questions of aesthetics and cultural acceptance. The fungal mycelium material qualities – antibacterial, fire-resistant, heat isolating and water-resistant – are incorporated in the function of each design. The objects adopt antique symbols embedding them in a longer material history, as well as a scale and form that introduces the fungal material to the context of the home. While the collection attempts to stress longevity and resilience, it is integrated into a circular vision, where the material is sourced from nature and returns to nature. MYCELIUM MILLENNIUM is an invitation to raise awareness for a material revolution, an opportunity to learn from nature and its potential, where products and objects could match the planet's needs.
Subjects/Keywords: mycelium; fungi; millennium; biofabrication; craft; fungal technology; material revolution; circular design; Design; Design
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APA (6th Edition):
Guerreiro, M. P. (2020). MYCELIUM MILLENNIUM. (Thesis). University of Arts. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-7324
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Guerreiro, Maria Pita. “MYCELIUM MILLENNIUM.” 2020. Thesis, University of Arts. Accessed January 24, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-7324.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Guerreiro, Maria Pita. “MYCELIUM MILLENNIUM.” 2020. Web. 24 Jan 2021.
Vancouver:
Guerreiro MP. MYCELIUM MILLENNIUM. [Internet] [Thesis]. University of Arts; 2020. [cited 2021 Jan 24].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-7324.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Guerreiro MP. MYCELIUM MILLENNIUM. [Thesis]. University of Arts; 2020. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-7324
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
18.
Gill, Elisabeth Lauren.
Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly.
Degree: PhD, 2020, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/303298
► Lab-grown tissues have tremendous potential to accelerate drug discovery and identify some of the underlying mechanisms behind diseases. The native extracellular matrix (ECM) of tissues…
(more)
▼ Lab-grown tissues have tremendous potential to accelerate drug discovery and identify some of the underlying mechanisms behind diseases. The native extracellular matrix (ECM) of tissues is a complex, hierarchical fibrous protein structure with delicate mechanical properties that guides tissue assembly and regeneration. Existing biomaterial fabrication techniques struggle to simultaneously attain: micro/nano-scale fibril feature resolution, low bulk stiffness and the 3D organisation crucially provided by the ECM without comprising cell motility. This work utilises 3D printing and low voltage electrospinning patterning synergistically to address these conflicting engineering challenges and act as a minimalist guide for 3D cell growth. A version of low voltage electrospinning patterning was adapted as a sequential process on a modified 3D printer. Applied voltage and 3D printed geometry can modulate the suspended behaviour of electrospun fibres that span between 3D printed support pillars, a parametric study characterised threshold conditions and established a predictive model for patterning suspended fibres. The accuracy with which suspended fibres followed the in-plane tool path was also assessed. Scanning Electron Microscopy imaging measured fibre diameters 1-5 μm and mechanical testing examines the properties for a given layer of dry fibres. The configuration demonstrated unique patterning of stacked suspended fibre layers in multiple orientations. Tissue scaffolding applications were explored in 2D and 3D. In 2D, gelatin fibres were patterned as a topographic cue to direct mesenchymal stem cells towards the osteogenic lineage. For 3D cell culture, the use of suspended fibre devices was investigated to improve the efficiency of cerebral organoid assembly. Pursuing these applications led to further refinement of the fibre fabrication technique and the development of targeted cell seeding strategies on suspended fibre structures. Glioblastoma cell aggregates were cultured on suspended fibre devices. Fibres guided the outgrowth of cancer cells from the aggregates, mimicking the topography of white matter tracts that assist migration in vivo. Cells assemble into dense (~200 μm depth) tissue structures with necrotic cores, that can remodel the fibre network yet are guided by the underlying fibre organisation. This novel method of patterning suspended microfibres from solution offers several avenues of inquiry to mimic ECM topography and complex material functionality.
Subjects/Keywords: electrospinning; biofabrication; 3d printing; 3d cell culture; suspended fibre patterning; low voltage
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APA (6th Edition):
Gill, E. L. (2020). Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/303298
Chicago Manual of Style (16th Edition):
Gill, Elisabeth Lauren. “Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly.” 2020. Doctoral Dissertation, University of Cambridge. Accessed January 24, 2021.
https://www.repository.cam.ac.uk/handle/1810/303298.
MLA Handbook (7th Edition):
Gill, Elisabeth Lauren. “Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly.” 2020. Web. 24 Jan 2021.
Vancouver:
Gill EL. Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly. [Internet] [Doctoral dissertation]. University of Cambridge; 2020. [cited 2021 Jan 24].
Available from: https://www.repository.cam.ac.uk/handle/1810/303298.
Council of Science Editors:
Gill EL. Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly. [Doctoral Dissertation]. University of Cambridge; 2020. Available from: https://www.repository.cam.ac.uk/handle/1810/303298
University of Maryland
19.
Betz, Jordan.
New Sensing Modalities for Bacterial and Environmental Phenomena.
Degree: Bioengineering, 2013, University of Maryland
URL: http://hdl.handle.net/1903/14814
► Intercellular communication is a ubiquitous phenomenon across all domains of life, ranging from archaea to bacteria to eukarya. In bacteria, this is often achieved using…
(more)
▼ Intercellular communication is a ubiquitous phenomenon across all domains of life, ranging from archaea to bacteria to eukarya. In bacteria, this is often achieved using small molecules that allow bacteria to sense and respond to environmental cues about the presence, identity, and number of neighboring bacteria. This confers survival and competitive advantages to bacteria by providing a coordinated, population-scale response to a given stimulus in the environment.
This dissertation describes the development of a microfluidic system for immobilizing and culturing of cells that also enables control over the genetic composition of the bacteria and their subsequent response to environmental stimuli via a new nonviral nucleic acid delivery mechanism. This nonviral nucleic acid delivery occurs outside the parameter space of traditional nonviral nucleic acid delivery methods such as electroporation and chemical transformation. The bacteria are immobilized in an optically clear alginate hydrogel which simulates the physical and chemical environment normally experienced by bacteria in a biofilm.
Complementing the microfluidic cell culture work, surface enhanced Raman spectroscopy (SERS), a label-free vibrational spectroscopic technique that lends itself well to use in aqueous systems, was used to detect bacterial signaling molecules. SERS was performed with three different examples of bacterial communication molecules: the universal quorum sensing molecule autoinducer-2 (AI-2), the species-specific Pseudomonas Quinolone Signal (PQS), and the stationary phase indicator molecule indole. SERS substrates were formed by galvanic displacement, a substrate fabrication method that can be adapted to many SERS applications.
Taken together, these new sensing modalities represent a step toward developing systems that allow researchers to investigate, understand, and ultimately control a cell's response to its environment.
Advisors/Committee Members: Rubloff, Gary W (advisor).
Subjects/Keywords: Biomedical engineering; Nanotechnology; autoinducer-2; biofabrication; microfluidics; quorum sensing; surface enhanced Raman spectroscopy; transformation
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APA (6th Edition):
Betz, J. (2013). New Sensing Modalities for Bacterial and Environmental Phenomena. (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/14814
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Betz, Jordan. “New Sensing Modalities for Bacterial and Environmental Phenomena.” 2013. Thesis, University of Maryland. Accessed January 24, 2021.
http://hdl.handle.net/1903/14814.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Betz, Jordan. “New Sensing Modalities for Bacterial and Environmental Phenomena.” 2013. Web. 24 Jan 2021.
Vancouver:
Betz J. New Sensing Modalities for Bacterial and Environmental Phenomena. [Internet] [Thesis]. University of Maryland; 2013. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/1903/14814.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Betz J. New Sensing Modalities for Bacterial and Environmental Phenomena. [Thesis]. University of Maryland; 2013. Available from: http://hdl.handle.net/1903/14814
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
20.
Li, Zhaoying.
Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues.
Degree: PhD, 2017, University of Cambridge
URL: https://doi.org/10.17863/CAM.17211
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.744415
► Recreating organ-specific microenvironments of the extracellular matrix (ECM) in vitro has been an ongoing challenge in biofabrication. In this study, I present a biofunctional ECM-mimicking…
(more)
▼ Recreating organ-specific microenvironments of the extracellular matrix (ECM) in vitro has been an ongoing challenge in biofabrication. In this study, I present a biofunctional ECM-mimicking protein scaffold with tunable biochemical, mechanical and topographical properties. This scaffold, formed by microfibres, displays three favorable characteristics as a cell culture platform: high-loading of key ECM proteins, single-layered mesh membrane with controllable mesh size, and flexibility for supporting a range of cell culture configurations. Decellularized extracellular matrix (dECM) powder was used to fabricate this protein scaffold, as a close replicate of the chemical composition of physiological ECM. The highest dECM concentration in the solidified protein scaffold was 50 wt%, with gelatin consisting the rest. In practice, a high density of dECM-laden nano- to microfibres was directly patterned on a variety of substrates to form a single layer of mesh membrane, using the low-voltage electrospinning patterning (LEP) method. The smallest fibre diameter was measured at 450 nm, the smallest mesh size of the membrane was below 1 μm, and the thickness of the membrane was estimated to be less than 2 μm. This fabrication method demonstrated a good preservation of the key ECM proteins and growth factors, including collagen IV, laminin, fibronectin, VEGF and b-FGF. The integrated fibrous mesh exhibited robust mechanical properties, with tunable fibril Young’s modulus for over two orders of magnitude in the physiological range (depending on the dECM concentration). Combining this mesh membrane with 3D printing, a cell culture device was constructed. Co-culture of human glomerulus endothelial cells and podocytes was performed on this device, to simulate the blood-to-urine interface in vitro. Good cell attachment and viability were demonstrated, and specific cell differentiation and fibronectin secretion were observed. This dECM-laden protein scaffold sees the potential to be incorporated into a glomerulus-on-chip model, to further improve the physiological relevance of in vitro pathological models.
Subjects/Keywords: 610.28; Decellularized extracellular matrix; Biofabrication; Microfibre; Protein scaffold; Electrospinning; Glomerulus; Kidney; Bioengineering
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APA ·
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Export
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APA (6th Edition):
Li, Z. (2017). Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.17211 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.744415
Chicago Manual of Style (16th Edition):
Li, Zhaoying. “Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues.” 2017. Doctoral Dissertation, University of Cambridge. Accessed January 24, 2021.
https://doi.org/10.17863/CAM.17211 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.744415.
MLA Handbook (7th Edition):
Li, Zhaoying. “Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues.” 2017. Web. 24 Jan 2021.
Vancouver:
Li Z. Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues. [Internet] [Doctoral dissertation]. University of Cambridge; 2017. [cited 2021 Jan 24].
Available from: https://doi.org/10.17863/CAM.17211 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.744415.
Council of Science Editors:
Li Z. Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues. [Doctoral Dissertation]. University of Cambridge; 2017. Available from: https://doi.org/10.17863/CAM.17211 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.744415
McMaster University
21.
Shahin-Shamsabadi, Alireza.
DEVELOPMENT OF BIOFABRICATION TECHNIQUES TO ENGINEER 3D IN VITRO AVATARS OF TISSUES.
Degree: PhD, 2020, McMaster University
URL: http://hdl.handle.net/11375/25796
► Two-dimensional (2D) in vitro models of tissues and organs have long been used as one of the main tools to understand human physiology and for…
(more)
▼ Two-dimensional (2D) in vitro models of tissues and organs have long been used as one of the main tools to understand human physiology and for applications such as drug discovery. But there is a huge disparity between in vivo conditions and these models which has created the need for better models. It has been shown that making three-dimensional models with dynamic environments that provide proper physical and chemical cues for cells, can bridge this gap between 2D models and in vivo conditions but the toolbox for creating such models has been imperfect and rudimentary. Introduction of tissue engineering concept and advent of biofabrication tools to meet its demands has provided new possible avenues for in vitro modeling but many of these tools are specifically designed to create tissue and organ replacements and lack features such as the ability to investigate cellular behavior with ease that are necessary for in vitro modeling purposes. The objective of this doctoral thesis was to introduce a novel toolbox of biofabrication techniques, based on bioprinting and bioassembly, that together are capable of recapitulating anatomical and physiological requirements of different tissue in in vitro setups in a more relevant way while creating the possibility of investigating cellular behavior. A bioprinting technique was developed that allowed formation of large constructs with proper mechanical stability, perfusion, and direct access to cells in different locations. The second technique was based on bioassembly of collagenous grafts in micro-molds and cells from different tissues with the ability to control cell positioning and create tissue-relevant cell densities with higher degree of similarity to human tissues compared to previous techniques. The third technique was based on bioassembled stand alone and dense cell-sheets for cells capable of fusion. These techniques were subsequently used for modeling a few chosen biological phenomenon to showcase the advantages of the techniques over previously developed ones and to further shed light on possible shortcomings of each of the techniques in their application for those specific tissues. In conclusion, our techniques may serve as valuable and easy to use tools for researchers, specifically biologists to investigate different aspects of human biology and disease mechanism in more details.
Thesis
Doctor of Philosophy (PhD)
Experimentation on humans is unethical, therefore in order to understand how human body works and test new therapeutic drugs researchers have used animals and cells isolated from animals or humans. Animals are inherently different from humans and isolated cells are culture in conditions different than human body, therefore a huge gap exists between the knowledge derived from these models and what happens in human body. Since there is no one-size-fits-all technique to model all of the human tissues, the objective of current study was set to build a toolbox of techniques that each could create better environment in the lab for cells isolated from…
Advisors/Committee Members: Selvaganapathy, Ponnambalam Ravi, Biomedical Engineering.
Subjects/Keywords: Biomedical Engineering; Tissue Engineering; 3D in vitro models; Biofabrication; Bioprinting; Bioassembly; Dynamic models
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APA ·
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APA (6th Edition):
Shahin-Shamsabadi, A. (2020). DEVELOPMENT OF BIOFABRICATION TECHNIQUES TO ENGINEER 3D IN VITRO AVATARS OF TISSUES. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/25796
Chicago Manual of Style (16th Edition):
Shahin-Shamsabadi, Alireza. “DEVELOPMENT OF BIOFABRICATION TECHNIQUES TO ENGINEER 3D IN VITRO AVATARS OF TISSUES.” 2020. Doctoral Dissertation, McMaster University. Accessed January 24, 2021.
http://hdl.handle.net/11375/25796.
MLA Handbook (7th Edition):
Shahin-Shamsabadi, Alireza. “DEVELOPMENT OF BIOFABRICATION TECHNIQUES TO ENGINEER 3D IN VITRO AVATARS OF TISSUES.” 2020. Web. 24 Jan 2021.
Vancouver:
Shahin-Shamsabadi A. DEVELOPMENT OF BIOFABRICATION TECHNIQUES TO ENGINEER 3D IN VITRO AVATARS OF TISSUES. [Internet] [Doctoral dissertation]. McMaster University; 2020. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/11375/25796.
Council of Science Editors:
Shahin-Shamsabadi A. DEVELOPMENT OF BIOFABRICATION TECHNIQUES TO ENGINEER 3D IN VITRO AVATARS OF TISSUES. [Doctoral Dissertation]. McMaster University; 2020. Available from: http://hdl.handle.net/11375/25796
22.
Schuddeboom, M.
Biofabrication of Perfusable Liver Constructs.
Degree: 2015, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/322746
Subjects/Keywords: Liver; Biofabrication; Bioprinting; Constructs; Organoids; MSC; Bioreactor; Perfusion
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APA ·
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APA (6th Edition):
Schuddeboom, M. (2015). Biofabrication of Perfusable Liver Constructs. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/322746
Chicago Manual of Style (16th Edition):
Schuddeboom, M. “Biofabrication of Perfusable Liver Constructs.” 2015. Masters Thesis, Universiteit Utrecht. Accessed January 24, 2021.
http://dspace.library.uu.nl:8080/handle/1874/322746.
MLA Handbook (7th Edition):
Schuddeboom, M. “Biofabrication of Perfusable Liver Constructs.” 2015. Web. 24 Jan 2021.
Vancouver:
Schuddeboom M. Biofabrication of Perfusable Liver Constructs. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Jan 24].
Available from: http://dspace.library.uu.nl:8080/handle/1874/322746.
Council of Science Editors:
Schuddeboom M. Biofabrication of Perfusable Liver Constructs. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/322746
23.
Tian, Xiaoyu.
Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage.
Degree: 2013, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2013-11-1388
► Tissue engineering is an emerging field aimed to combine biological, engineering and material methods to create a biomimetic three dimensional (3D) environment to control cells…
(more)
▼ Tissue engineering is an emerging field aimed to combine biological, engineering and material methods to create a biomimetic three dimensional (3D) environment to control cells proliferation and functional tissue formation. In such an artificial structural environment, a scaffold, made from biomaterial(s), plays an essential role by providing a mechanical support and biological guidance platform. Hence, fabrication of tissue scaffolds is of a fundamental importance, yet a challenging task, in tissue engineering. This task becomes more challenging if living cells need to be encapsulated in the scaffolds so as to fabricate scaffolds with structures to mimic the native ones, mainly due to the issue of process-induced cell damage. This research aims to develop novel methods to model the process of fabricating cell-encapsulated scaffolds and process-induced cell damage. Particularly, this research focuses on the scaffold fabrication process based on the dispensing-based rapid prototyping technique - one of the most promising scaffold fabrication methods nowadays, by which a 3D scaffold is fabricated by laying down multiple, precisely formed layers in succession.
In the dispensing-based scaffold fabrication process, the flow behavior of biomaterials solution can significantly affect the flow rate of material dispensed, thus the structure of scaffold fabricated. In this research, characterization of flow behavior of materials was studied; and models to represent the flow behaviour and its influence on the scaffold structure were developed. The resultant models were shown able to greatly improve the scaffold fabrication in terms of process parameter determination.
If cells are encapsulated in hydrogel for scaffold fabrication, cell density can affect the mechanical properties of hydrogel scaffolds formed. In this research, the influence of cell density on mechanical properties of hydrogel scaffolds was investigated. Furthermore, finite element analysis (FEA) of mechanical properties of scaffolds with varying cell densities was performed.The results show that the local stress and strain energy on cells varies at different cell densities. The method developed may greatly facilitate hydrogel scaffolds design to minimize cell damage in scaffold and promote tissue regeneration. .
In the cell-encapsulated scaffold fabrication process, cells inevitably suffer from mechanical forces and other process-induced hazards. In such a harsh environment, cells deform and may be injured, even damaged due to mechanical breakage of cell membrane. In this research, three primary physical variables: shear stress, exposure time, and temperature were examined and investigated with regard to their effects on cell damage. Cell damage laws through the development phenomenal models and computational fluidic dynamic (CFD) models were established; and their applications to the cell-encapsulated scaffold fabrication process were pursued. The results obtained show these models and modeling methods not only allow one to optimize process parameters to…
Subjects/Keywords: Tissue Engineering; Biofabrication; cell damage; tissue scaffold
…BIOFABRICATION PROCESS… …87
CHAPTER 7 TEMPERATURE EFFECT ON THE SHEAR-INDUCED CELL DAMAGE IN
BIOFABRICATION… …91
7.2.5
Biofabrication system… …95
7.3.3
Applications to the biofabrication process… …biofabrication techniques ..................................................... 20
Table 3. 1 Flow…
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APA (6th Edition):
Tian, X. (2013). Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2013-11-1388
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tian, Xiaoyu. “Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage.” 2013. Thesis, University of Saskatchewan. Accessed January 24, 2021.
http://hdl.handle.net/10388/ETD-2013-11-1388.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tian, Xiaoyu. “Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage.” 2013. Web. 24 Jan 2021.
Vancouver:
Tian X. Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage. [Internet] [Thesis]. University of Saskatchewan; 2013. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/10388/ETD-2013-11-1388.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tian X. Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage. [Thesis]. University of Saskatchewan; 2013. Available from: http://hdl.handle.net/10388/ETD-2013-11-1388
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Tech
24.
Sano, Michael Benjamin.
Electromagnetic Control of Biological Assembly.
Degree: MS, Engineering Science and Mechanics, 2010, Virginia Tech
URL: http://hdl.handle.net/10919/76975
► We have developed a new biofabrication process in which the precise control of bacterial motion is used to fabricate customizable networks of cellulose nanofibrils. This…
(more)
▼ We have developed a new
biofabrication process in which the precise control of bacterial motion is used to fabricate customizable networks of cellulose nanofibrils. This work describes how the motion of Acetobacter xylinum can be controlled by electric fields while the bacteria simultaneously produce nanocellulose, resulting in networks with aligned fibers. Since the electrolysis of water due to the application of electric fields produces the oxygen in the culture media far from the liquid-air boundary, aerobic cellulose production in 3D structures is readily achievable. Five separate sets of experiments were conducted to demonstrate the assembly of nanocellulose by Acetobacter xylinum in the presence of electric fields in micro and macro environments. This work demonstrates a new concept of bottom up material synthesis by control of a biological assembly process.
Advisors/Committee Members: Davalos, Rafael V. (committeechair), Gatenholm, Paul (committee member), Socha, John J. (committee member).
Subjects/Keywords: Acetobacter xylinum; Directed biofabrication; Electrokinetics; Biological assembly
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APA (6th Edition):
Sano, M. B. (2010). Electromagnetic Control of Biological Assembly. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/76975
Chicago Manual of Style (16th Edition):
Sano, Michael Benjamin. “Electromagnetic Control of Biological Assembly.” 2010. Masters Thesis, Virginia Tech. Accessed January 24, 2021.
http://hdl.handle.net/10919/76975.
MLA Handbook (7th Edition):
Sano, Michael Benjamin. “Electromagnetic Control of Biological Assembly.” 2010. Web. 24 Jan 2021.
Vancouver:
Sano MB. Electromagnetic Control of Biological Assembly. [Internet] [Masters thesis]. Virginia Tech; 2010. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/10919/76975.
Council of Science Editors:
Sano MB. Electromagnetic Control of Biological Assembly. [Masters Thesis]. Virginia Tech; 2010. Available from: http://hdl.handle.net/10919/76975
25.
Kérourédan, Olivia.
Effet de la pré-vascularisation organisée par Bioimpression Assistée par Laser sur la régénération osseuse : Effect of prevascularization designed by Laser-Assisted Bioprinting on bone regeneration.
Degree: Docteur es, Biologie Cellulaire et Physiopathologie, 2019, Bordeaux
URL: http://www.theses.fr/2019BORD0028
► Afin de résoudre la problématique des substituts osseux faiblement vascularisés, un des challenges majeurs en ingénierie tissulaire osseuse est de favoriser le développement précoce d’une…
(more)
▼ Afin de résoudre la problématique des substituts osseux faiblement vascularisés, un des challenges majeurs en ingénierie tissulaire osseuse est de favoriser le développement précoce d’une microvascularisation. La reproduction du microenvironnement local et l’organisation cellulaire in situ sont des approches innovantes pour optimiser la formation osseuse. En Biofabrication, la Bioimpression Assistée par Laser (LAB) est une technologie émergente permettant l’impression de cellules et de biomatériaux avec une résolution micrométrique. L’objectif de ce travail était d’étudier l’effet de l’organisation de la pré-vascularisation par LAB sur la régénération osseuse. La station de bioimpression Novalase a été utilisée pour imprimer des motifs de cellules endothéliales sur un « biopaper » constitué de collagène et de cellules souches issues de la papille apicale. Les paramètres d’impression, densités cellulaires et conditions de recouvrement ont été optimisés afin de favoriser la formation d’un réseau microvasculaire avec une architecture définie in vitro. Ce modèle a ensuite été transposé in vivo, grâce à la bioimpression in situ de cellules endothéliales au niveau de défauts osseux critiques chez la souris, afin d’évaluer si la prévascularisation organisée par LAB permettait de promouvoir et contrôler spatialement le processus de régénération osseuse. Les résultats ont montré que la bioimpression permettait d’augmenter la densité de vaisseaux dans les défauts osseux et de favoriser la régénération osseuse.
In order to solve the issue of poorly vascularized bone substitutes, development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. The reproduction of local microenvironment and in situ organization of cells are innovating approaches to optimize bone formation. In Biofabrication, Laser-Assisted Bioprinting (LAB) has emerged as a relevant method to print living cells and biomaterials with micrometric resolution. The aim of this work was to study the effect of prevascularization organized by LAB on bone regeneration. The laser workstation Novalase was used to print patterns of endothelial cells onto a « biopaper » of collagen hydrogel seeded with stem cells from the apical papilla. Printing parameters, cell densities and overlay conditions were optimized to enhance the formation of microvascular networks with a defined architecture in vitro. This model was then transposed in vivo, through in situ bioprinting of endothelial cells into mouse calvarial bone defects of critical size, to investigate if prevascularization organized by LAB can promote and spatially control bone regeneration. The results showed that bioprinting allowed to increase blood vessel density in bone defects and promote bone regeneration.
Advisors/Committee Members: Devillard, Raphaël (thesis director).
Subjects/Keywords: Ingénierie tissulaire osseuse; Biofabrication; Bioimpression assistée par laser; Vascularisation; Progéniteurs endothéliaux; Cellules souches issues de la papille apicale; Bone tissue engineering; Biofabrication; Laser-Assisted bioprinting; Vascularization; Endothelial progenitors; Stem cells from the apical papilla
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APA ·
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APA (6th Edition):
Kérourédan, O. (2019). Effet de la pré-vascularisation organisée par Bioimpression Assistée par Laser sur la régénération osseuse : Effect of prevascularization designed by Laser-Assisted Bioprinting on bone regeneration. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2019BORD0028
Chicago Manual of Style (16th Edition):
Kérourédan, Olivia. “Effet de la pré-vascularisation organisée par Bioimpression Assistée par Laser sur la régénération osseuse : Effect of prevascularization designed by Laser-Assisted Bioprinting on bone regeneration.” 2019. Doctoral Dissertation, Bordeaux. Accessed January 24, 2021.
http://www.theses.fr/2019BORD0028.
MLA Handbook (7th Edition):
Kérourédan, Olivia. “Effet de la pré-vascularisation organisée par Bioimpression Assistée par Laser sur la régénération osseuse : Effect of prevascularization designed by Laser-Assisted Bioprinting on bone regeneration.” 2019. Web. 24 Jan 2021.
Vancouver:
Kérourédan O. Effet de la pré-vascularisation organisée par Bioimpression Assistée par Laser sur la régénération osseuse : Effect of prevascularization designed by Laser-Assisted Bioprinting on bone regeneration. [Internet] [Doctoral dissertation]. Bordeaux; 2019. [cited 2021 Jan 24].
Available from: http://www.theses.fr/2019BORD0028.
Council of Science Editors:
Kérourédan O. Effet de la pré-vascularisation organisée par Bioimpression Assistée par Laser sur la régénération osseuse : Effect of prevascularization designed by Laser-Assisted Bioprinting on bone regeneration. [Doctoral Dissertation]. Bordeaux; 2019. Available from: http://www.theses.fr/2019BORD0028
26.
Visser, J.
Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.
Degree: 2015, University Utrecht
URL: http://dspace.library.uu.nl/handle/1874/318088
;
URN:NBN:NL:UI:10-1874-318088
;
urn:isbn:978-94-6169-706-6
;
URN:NBN:NL:UI:10-1874-318088
;
http://dspace.library.uu.nl/handle/1874/318088
► Implants were biofabricated for the repair of chondral and osteochondral articular joint defects. The implants were based on gelatin methacrylamide (GelMA) hydrogels combined with printed…
(more)
▼ Implants were biofabricated for the repair of chondral and osteochondral articular joint defects. The implants were based on gelatin methacrylamide (GelMA) hydrogels combined with printed fibers from polycaprolactone (PCL) for mechanical reinforcement. In Part I of the thesis, biological modifications of GelMA by the addition of matrix derived from cartilage, meniscus or tendon tissue did not show a positive effect on in vitro cartilage matrix production by encapsulated chondrocytes or MSCs. Furthermore, MSCs in GelMA in a subcutaneous rat model could not be locked in their chondrogenic state, considering the evident process of endochondral bone formation in these constructs. Both the quantity and quality of bone formed by MSCs in GelMA are nonetheless encouraging for bone tissue engineers. In Part II, hydrogels were successfully reinforced with PCL microfibers. These hydrogel/microfiber composites approached the stiffness and elasticity of articular cartilage and permitted the formation of cartilage matrix by embedded chondrocytes. The repair of focal cartilage defects with reinforced GelMA gels is currently evaluated in eight Shetland ponies, with a follow-up of one year, including several arthroscopic imaging techniques and standardized gait analysis. In Part III, reinforced GelMA was applied for the
biofabrication of implants for the restoration of large joint defects. Anatomically-shaped, osteochondral implants were fabricated and further developed for the restoration of the complete shoulder joint in rabbits. A pilot study on the strength and fixation of the implants in ongoing in three rabbits. Altogether, in this thesis, biologically active implants have been biofabricated with an imposed internal organization and external architecture. The regeneration of bone and cartilage tissue in these constructs was demonstrated in vitro and in ectopic locations in rodents. Large animal models were established and are currently conducted to evaluate the potential of these implants for the repair of focal or complete articular joint defects.
Advisors/Committee Members: Dhert, W.J.A., Malda, J., Gawlitta, D..
Subjects/Keywords: biofabrication; 3D-printing; Bone; cartilage; tissue-engineering; hydrogel; implant; biomaterials; orthopedics
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APA (6th Edition):
Visser, J. (2015). Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. (Doctoral Dissertation). University Utrecht. Retrieved from http://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; http://dspace.library.uu.nl/handle/1874/318088
Chicago Manual of Style (16th Edition):
Visser, J. “Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.” 2015. Doctoral Dissertation, University Utrecht. Accessed January 24, 2021.
http://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; http://dspace.library.uu.nl/handle/1874/318088.
MLA Handbook (7th Edition):
Visser, J. “Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.” 2015. Web. 24 Jan 2021.
Vancouver:
Visser J. Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. [Internet] [Doctoral dissertation]. University Utrecht; 2015. [cited 2021 Jan 24].
Available from: http://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; http://dspace.library.uu.nl/handle/1874/318088.
Council of Science Editors:
Visser J. Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. [Doctoral Dissertation]. University Utrecht; 2015. Available from: http://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; http://dspace.library.uu.nl/handle/1874/318088
27.
Pagandiaz, Gelson J.
Biofabrication of muscular and neuronal in-vitro tissue for multi-cellular engineered living systems.
Degree: PhD, Bioengineering, 2020, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/107859
► The ability of designing, biofabricating and programming engineered tissue constructs, as well as modularly assembling them to achieve the engineering of heterotypic living systems is…
(more)
▼ The ability of designing, biofabricating and programming engineered tissue constructs, as well as modularly assembling them to achieve the engineering of heterotypic living systems is a key element for the progress of “New Biology”. This perspective consists of utilizing engineering concepts for design of complex systems and combining with the principles and properties of multiple disciplines in cellular biology at various length scales to drive composition of living material that are organized in a way that allows for the emergence of novel functionalities. Advances in this field can constitute an experimental platform that can guide the discovery of factors that allow the emergence of properties that can translate into a richer toolbox with which to address real world problems.
One important example is the development of biological machines, namely consisting of motor neurons as a source of processing and activation and skeletal muscle as the actuator of the system. Towards this goal, advances in the generation of a neuro-muscular driven machine require an expansion of design parameters and arrangement of the assembled parts, guided by parameters established by natural processes. This dissertation presents advances on the expansion of this toolbox for the engineering of multicellular living systems consisting of muscle calls and motor neurons. The dissertation focuses on the modulation of the electrical activity of motor neuronal networks, the
biofabrication of neural tissue containing motor neurons, and the redesigning of previous muscle-based walking robots for higher force generation.
First of all, the ability to uncover in-vitro methods to “reprogram” neural networks responsible for guiding the actuation of muscle is critical to engineer the dynamic functionalities needed in these biological machines. In this work, motor neurons were differentiated from mouse embryonic stem cells, which have been transfected to express a GFP reporter under the Hb9 promoter as well as Channelrhodopsin, to enable optogenetic stimulation of these neurons, which was used to design the training regimens. Past efforts have not considered reprogramming in terms of evoking long-term changes in firing patterns of in-vitro networks by training regimens during stages of neural development. Thus, in this dissertation, short and long-term programming of neural networks was explored by using optical stimulation to induce training regimens implemented during neurogenesis and synaptogenesis, and ultimately demonstrating correlation between these and the resulting plastic responses.
Furthermore, to design multicellular engineered living systems subunits that could facilitate the assembly of heterotypic biological machines, a novel
biofabrication approach is proposed to form functional in-vitro neural tissue mimics (NTM) using mouse embryonic stem cells, a fibrin matrix, and 3D printed molds. This method can provide a large degree of design flexibility for development of in-vitro functional neural tissue models of varying shapes and forms…
Advisors/Committee Members: Bashir, Rashid (advisor), Bashir, Rashid (Committee Chair), Gillette, Martha (committee member), Kong, Joon (committee member), Gazzola, Mattia (committee member).
Subjects/Keywords: biofabrication; mESC; skeletal muscle; neural tissue; engineered living systems
…possible by advances in biofabrication
techniques. These machines have mostly involved the… …contractions (11). Furthermore, this
initial biofabrication approach involved forming 2D… …in
biofabrication methodology for the engineering of in-vitro biological machines… …formation.
The Chapter 3 presents the development of a protocol to enable the biofabrication of… …Raman, R. Bashir, Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence
and…
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Pagandiaz, G. J. (2020). Biofabrication of muscular and neuronal in-vitro tissue for multi-cellular engineered living systems. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/107859
Chicago Manual of Style (16th Edition):
Pagandiaz, Gelson J. “Biofabrication of muscular and neuronal in-vitro tissue for multi-cellular engineered living systems.” 2020. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 24, 2021.
http://hdl.handle.net/2142/107859.
MLA Handbook (7th Edition):
Pagandiaz, Gelson J. “Biofabrication of muscular and neuronal in-vitro tissue for multi-cellular engineered living systems.” 2020. Web. 24 Jan 2021.
Vancouver:
Pagandiaz GJ. Biofabrication of muscular and neuronal in-vitro tissue for multi-cellular engineered living systems. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2020. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/2142/107859.
Council of Science Editors:
Pagandiaz GJ. Biofabrication of muscular and neuronal in-vitro tissue for multi-cellular engineered living systems. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2020. Available from: http://hdl.handle.net/2142/107859
28.
Visser, J.
Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.
Degree: 2015, University Utrecht
URL: https://dspace.library.uu.nl/handle/1874/318088
;
URN:NBN:NL:UI:10-1874-318088
;
urn:isbn:978-94-6169-706-6
;
URN:NBN:NL:UI:10-1874-318088
;
https://dspace.library.uu.nl/handle/1874/318088
► Implants were biofabricated for the repair of chondral and osteochondral articular joint defects. The implants were based on gelatin methacrylamide (GelMA) hydrogels combined with printed…
(more)
▼ Implants were biofabricated for the repair of chondral and osteochondral articular joint defects. The implants were based on gelatin methacrylamide (GelMA) hydrogels combined with printed fibers from polycaprolactone (PCL) for mechanical reinforcement. In Part I of the thesis, biological modifications of GelMA by the addition of matrix derived from cartilage, meniscus or tendon tissue did not show a positive effect on in vitro cartilage matrix production by encapsulated chondrocytes or MSCs. Furthermore, MSCs in GelMA in a subcutaneous rat model could not be locked in their chondrogenic state, considering the evident process of endochondral bone formation in these constructs. Both the quantity and quality of bone formed by MSCs in GelMA are nonetheless encouraging for bone tissue engineers. In Part II, hydrogels were successfully reinforced with PCL microfibers. These hydrogel/microfiber composites approached the stiffness and elasticity of articular cartilage and permitted the formation of cartilage matrix by embedded chondrocytes. The repair of focal cartilage defects with reinforced GelMA gels is currently evaluated in eight Shetland ponies, with a follow-up of one year, including several arthroscopic imaging techniques and standardized gait analysis. In Part III, reinforced GelMA was applied for the
biofabrication of implants for the restoration of large joint defects. Anatomically-shaped, osteochondral implants were fabricated and further developed for the restoration of the complete shoulder joint in rabbits. A pilot study on the strength and fixation of the implants in ongoing in three rabbits. Altogether, in this thesis, biologically active implants have been biofabricated with an imposed internal organization and external architecture. The regeneration of bone and cartilage tissue in these constructs was demonstrated in vitro and in ectopic locations in rodents. Large animal models were established and are currently conducted to evaluate the potential of these implants for the repair of focal or complete articular joint defects.
Advisors/Committee Members: Dhert, W.J.A., Malda, J., Gawlitta, D..
Subjects/Keywords: biofabrication; 3D-printing; Bone; cartilage; tissue-engineering; hydrogel; implant; biomaterials; orthopedics
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Visser, J. (2015). Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; https://dspace.library.uu.nl/handle/1874/318088
Chicago Manual of Style (16th Edition):
Visser, J. “Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.” 2015. Doctoral Dissertation, University Utrecht. Accessed January 24, 2021.
https://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; https://dspace.library.uu.nl/handle/1874/318088.
MLA Handbook (7th Edition):
Visser, J. “Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels.” 2015. Web. 24 Jan 2021.
Vancouver:
Visser J. Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. [Internet] [Doctoral dissertation]. University Utrecht; 2015. [cited 2021 Jan 24].
Available from: https://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; https://dspace.library.uu.nl/handle/1874/318088.
Council of Science Editors:
Visser J. Biofabrication of implants for articular joint repair : Cartilage regeneration in reinforced gelatin-based hydrogels. [Doctoral Dissertation]. University Utrecht; 2015. Available from: https://dspace.library.uu.nl/handle/1874/318088 ; URN:NBN:NL:UI:10-1874-318088 ; urn:isbn:978-94-6169-706-6 ; URN:NBN:NL:UI:10-1874-318088 ; https://dspace.library.uu.nl/handle/1874/318088
University of Otago
29.
Mekhileri, Naveen Vijayan.
Integration and automation of a micro-tissue and microsphere based tissue engineering system and its application in cartilage regeneration and cancer models
.
Degree: University of Otago
URL: http://hdl.handle.net/10523/7645
► Bottom-up biofabrication approaches for fabricating engineered tissue constructs are emerging strategies in tissue engineering. Few technologies have been developed that are capable of assembling tissue…
(more)
▼ Bottom-up
biofabrication approaches for fabricating engineered tissue constructs are emerging strategies in tissue engineering. Few technologies have been developed that are capable of assembling tissue units into 3D Plotted scaffolds. We developed an integrated and automated 3D Bioassembly system for bioassembling engineered tissue constructs. The developed automated bioassembly system consisted of a (i) singularisation module and (ii) an injection module integrated into a commercial 3D bioprinter. The fluidic-based singularisation module delivered single Ø1 mm sized tissue unit at a time to the injection module and the injection module together with the 3D positioning system of the 3D bioprinter delivered the tissue unit into a predefined pore in the 3D Plotted scaffold. The developed automated bioassembly system was capable of either fabricating a construct via a two-step top-down bioassembly approach (fabricating a complete scaffold and insertion of tissue units) or a multistep bottom-up bioassembly approach (alternative layer-by-layer scaffold fabrication and tissue unit co-assembly). The automated bioassembly system was validated for application in cartilage and tumour engineering using tissue units (microspheres and micro-tissues). For cartilage engineering, Ø1 mm sized cartilage micro-tissues were fabricated utilising a previously demonstrated high-throughput 96-well plate format and Ø1 mm sized chondrocytes or chondroprogenitor cells-laden GelMA (gelatin-methacryloyl)-HepMA (methacrylated heparin) (9.5%-0.5%) hydrogel microspheres were fabricated utilising an adopted microfluidic system. For tumour engineering, a co-culture of cancer cells with fibroblasts using a liquid overlay technique was required to fabricate compact spherical Ø1 mm micro-tissues that could be handled by the automated bioassembly system and cancer cell-laden 10% GelMA hydrogel microspheres were fabricated utilising the adopted microfluidic system. Reliable handling of the tissue units was demonstrated by the automated bioassembly system. Bottom-up bioassembly of tissue units into 3D Plotted PEGT/PBT polymer scaffolds was demonstrated with the automated bioassembly system. No difference in viability was observed between the constructs assembled manually and with the automated bioassembly system. The flexibility of the automated tissue bioassembly system was shown by assembling constructs with coloured microspheres (denoting microspheres of different types) in various desired arrangements. The automated bioassembly of an anatomically shaped construct was also demonstrated. Neocartilage formation was observed in the chondrocyte-laden individual microspheres and assembled constructs when cultured in vitro for 35 days. Neocartilage formation was also visualised in the assembled graduated constructs fabricated with human articular chondrocytes (HAC) and mesenchymal stromal cells (MSC). In the in vitro micro-tissue tumour model, individual micro-tissues had higher chemoresistance compared to cells in 2D and the co-culture assembled construct…
Advisors/Committee Members: Woodfield, Tim (advisor).
Subjects/Keywords: Tissue engineering;
Bioassembly;
Automated;
3D printing;
Cancer model;
Biofabrication;
Cartilage;
3D Plotting;
Bioprinting;
Cartilage regeneration
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APA (6th Edition):
Mekhileri, N. V. (n.d.). Integration and automation of a micro-tissue and microsphere based tissue engineering system and its application in cartilage regeneration and cancer models
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/7645
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Mekhileri, Naveen Vijayan. “Integration and automation of a micro-tissue and microsphere based tissue engineering system and its application in cartilage regeneration and cancer models
.” Doctoral Dissertation, University of Otago. Accessed January 24, 2021.
http://hdl.handle.net/10523/7645.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Mekhileri, Naveen Vijayan. “Integration and automation of a micro-tissue and microsphere based tissue engineering system and its application in cartilage regeneration and cancer models
.” Web. 24 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Mekhileri NV. Integration and automation of a micro-tissue and microsphere based tissue engineering system and its application in cartilage regeneration and cancer models
. [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Jan 24].
Available from: http://hdl.handle.net/10523/7645.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Mekhileri NV. Integration and automation of a micro-tissue and microsphere based tissue engineering system and its application in cartilage regeneration and cancer models
. [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/7645
Note: this citation may be lacking information needed for this citation format:
No year of publication.
30.
Zhu, Ning.
Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering.
Degree: 2012, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2012-05-465
► Tissue engineering emerges nowadays to seek new solutions to damaged tissues and/or organs by replacing or repairing them with engineered constructs or scaffolds. In nerve…
(more)
▼ Tissue engineering emerges nowadays to seek new solutions to damaged tissues and/or organs by replacing or repairing them with engineered constructs or scaffolds. In nerve tissue engineering, scaffolds for the repair of peripheral nerve injuries should act to support and promote axon growth following implantation. It is believed that substantial progress can be made by creating scaffolds from biomaterials, with growth-promoting molecules and spatially-controlled microstructure. To this end, this research aims to develop three dimensional (3D) scaffolds for peripheral nerve tissue regeneration by focusing on studies on the axon guidance, development and characterization of a novel 3D scaffold, and visualization of scaffolds by means of synchrotron-based diffraction enhanced imaging (DEI).
Axon guidance is one of crucial considerations in developing of nerve scaffolds for nerve regeneration. In order to study the axon guidance mechanism, a two dimensional (2D) grid micropatterns were created by dispensing chitosan or laminin-blended chitosan substrate strands oriented in orthogonal directions; and then used in the in vitro dorsal root ganglion (DRG) neuron culture experiments. The results show the effect of the micropatterns on neurite directional growth can preferentially grow upon and follow the laminin-blended chitosan pathways.
A novel 3D scaffold was developed for potential applications to peripheral nerve tissue engineering applications. The scaffolds were fabricated from poly L-lactide (PLLA) mixed with chitosan microspheres (CMs) by using a rapid freeze prototyping (RFP) technique, allowing for controllable scaffold microstructure and bioactivities protein release. The scaffold characterization shows that (1) the mechanical properties of the scaffolds depend on the ratio of CMs to PLLA as well as the cryogenic temperature and (2) the protein release can be controlled by adjusting the crosslink degree of the CMs and prolonged after the CMs were embedded into the PLLA scaffolds. Also, the degradation properties of the scaffolds were investigated with the results showing that the addition of CMs to PLLA can decrease the degradation rate as compared to pure PLLA scaffolds. This allows for another means to control the degradation rate.
Visualization of polymer scaffolds in soft tissues is challenging, yet essential, to the success of tissue engineering applications. The x-ray diffraction enhanced imaging (DEI) method was explored for the visualization of the PLLA/CMs scaffolds embedded in soft tissues. Among various methods examined, including conventional radiography and in-line phase contrast imaging techniques, the DEI was the only technique able to visualize the scaffolds embedded in unstained muscle tissue as well as the microstructure of muscle tissue. Also, it has been shown that the DEI has the capacity to image the scaffolds in thicker tissue, and reduce the radiation doses to tissues as compared to conventional radiography.
The methods and results developed/obtained in this study represent a…
Advisors/Committee Members: Chen, Daniel, Chapman, Dean, Gupta, Madan, Cooper, David, Niu, Catherine, Zhang, Chris.
Subjects/Keywords: Nerve tissue engineering; Scaffolds, Axon guidance; Biofabrication; X-ray imaging; Diffraction Enhanced Imaging
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APA (6th Edition):
Zhu, N. (2012). Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-05-465
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zhu, Ning. “Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering.” 2012. Thesis, University of Saskatchewan. Accessed January 24, 2021.
http://hdl.handle.net/10388/ETD-2012-05-465.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zhu, Ning. “Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering.” 2012. Web. 24 Jan 2021.
Vancouver:
Zhu N. Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Jan 24].
Available from: http://hdl.handle.net/10388/ETD-2012-05-465.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zhu N. Development and in vitro characterization of three dimensional biodegradable scaffolds for peripheral nerve tissue engineering. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-05-465
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations
