subject:(Biocatalytic membrane reactor)

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University of New South Wales

1. Ji, Chao. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.

Degree: Chemical Engineering, 2017, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true

The increasing worldwide contamination of freshwater system with micro-pollutants emerges as a critical environmental problem, which has driven the search for novel mitigation approaches. The use of enzymes such as laccase as biocatalyst has been recognised as a promising approach for micro-pollutants removal. However, rapid denaturation of the free enzyme and its difficulty in recycling and reuse restrict its wider application, and efficient enzyme immobilization and bioreactor design are required.In this study, and two membrane bioreactors were proposed for carbamazepine (CBZ) degradation: the hybrid membrane system where laccase-immobilized TiO2 nanoparticles were suspended in the feed solution, and the biocatalytic membrane reactor where laccase was immobilised on TiO2 coated membrane surface. Using p-coumaric acid as a mediator, efficient CBZ removal (up to 71%) was achieved with the hybrid membrane reactor. Functionalized TiO2 nanoparticles were further applied to immobilize crude enzyme extracts from P. ostreatus culture. The resultant biocatalytic particles had comparable performance to the immobilized purified commercial laccase and showed efficient bisphenol-A and CBZ removal in the hybrid reactor.In addition, a cross-linked carbon nanotubes (CNTs) based membrane was prepared, which exhibited high effectiveness as support for physical adsorption of laccase. The active laccase coating on CNTs membrane can be renewed after simple cleaning and re-immobilization. The biocatalytic membrane also showed substantial improvement in micro-pollutant removal compared with the membrane having no enzyme. At last, it is demonstrated that the intramolecular electron transfer within single enzyme molecule is an important alternative pathway which can be harnessed to generate electricity. By decoupling the redox reaction within laccase, efficient electricity production from unconventional fuels including recalcitrant pollutants and/or toxic organic was obtained in a sole-laccase based enzymatic fuel cell. The intramolecular electron-harnessing concept was also demonstrated with other enzymes, including the power generation during CO2 bioconversion to formate catalysed by formate dehydrogenase. The novel enzymatic power generation is shown to be potentially feasible utilizing wastewater as fuel as well as occurring in tandem with driving bioconversion of chemical feedstock from CO2. Advisors/Committee Members: Chen, Vicki, Chemical Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: Micro-pollutant; Laccase; Biocatalytic membrane reactor; Enzymatic fuel cell; Enzyme immobilization

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APA (6^th Edition):

Ji, C. (2017). Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Ji, Chao. “Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.” 2017. Doctoral Dissertation, University of New South Wales. Accessed January 16, 2021. http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Ji, Chao. “Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.” 2017. Web. 16 Jan 2021.

Vancouver:

Ji C. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2021 Jan 16]. Available from: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true.

Council of Science Editors:

Ji C. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true

University of Ottawa

2. Li, Aotian. Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors .

Degree: 2020, University of Ottawa

URL: http://hdl.handle.net/10393/40473

Biocatalytic membranes (BMs) have promising applications in a diversity of fields including food, pharmaceutical and water treatment industries. Of particular relevance, Alcalase is a commercially important protease that has been applied for the production of peptides from the hydrolysis of proteins. In this study, two different approaches were applied for the modification of electrospun polyacrylonitrile nanofibrous membranes (EPNMs) for Alcalase immobilization. The first approach is alkali modification of EPNMs followed by EDC/NHS coupling for covalent bonding with Alcalase, whereas the other is based on polydopamine coating with or without glutaraldehyde grafting as a covalent linker. Immobilized Alcalase on these prepared BMs were studied and compared with free enzymes. It was found that the stabilities of Alcalase on BMs created using both approaches were improved, which enabled their reuse of 10 cycles with significant retention of enzymatic activity. A continuous reactor housing BMs were tested for hydrolysis of both model substrate, azo-casein and soybean meal protein (SMP). It was found that decreasing flux could improve the extent of hydrolysis and that a single-layer reactor can hydrolyze about 50% of the substrate to peptides with the molecular weight of 10 kDa or less. Hydrolysis of SMPs was demonstrated in a continuous five-layer BM reactor and both BMs showed excellent hydrolysis capacity. This study provides the groundwork for the development of high-efficiency BM for continuous and cost-effective protein hydrolysis for the production of value-added peptides.

Subjects/Keywords: Biocatalytic Membrane; Enzyme Immobilization; Electrospun PAN Nanofibrous Membrane; Protein Hydrolysis; Continuous Reactor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, A. (2020). Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/40473

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Aotian. “Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors .” 2020. Thesis, University of Ottawa. Accessed January 16, 2021. http://hdl.handle.net/10393/40473.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Aotian. “Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors .” 2020. Web. 16 Jan 2021.

Vancouver:

Li A. Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors . [Internet] [Thesis]. University of Ottawa; 2020. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10393/40473.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li A. Development of Biocatalytic Nanofibrous Membranes Using Different Modification Approaches for Continuous Proteolytic Reactors . [Thesis]. University of Ottawa; 2020. Available from: http://hdl.handle.net/10393/40473

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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