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Universidade Estadual de Campinas

1. Domingos, Daniela Ferreira, 1984-. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.

Degree: 2014, Universidade Estadual de Campinas

Abstract: Mangrove is an environment rich in microbial diversity, but little has been reported about it in Brazil, making the knowledge and exploitation of new microorganisms in mangroves an imperative issue. The prospection of microbial diversity in underexplored environments, such as mangroves, increses possibility of success in looking for new bioactive molecules, contributing to a more sustentable economy and environment. Although the cultivation techniques have been improved, allowing an increased number of yet uncultivated microorganisms to be able recuperated in vitro, our knowledge about their ecology is still insufficient to cultivate the majority of them. Therefore, the metagenomic library have emerged as a powerful tool to more widely access the overall microbial diversity in an environment, enabling the functional analyses of genes and the discovery of new bioactive compounds, mainly uncultured-microorganisms. The aim of the current work was the prospection of biosurfactant from mangrove microbiota through a polifasica approach. For the independent-cultivation approach, a metagenomic library of high molecular weight was constructed from mangrove sediment contaminated with oil. The clones were subjected to functional and molecular screening to identify biosurfactant activity. Three clones with the potential to procuce biosurfactant were selected, and the fosmid sequencing for genetic chatacterization of the insert was carried out. Although the clones showed the ability to reduce the surface tension, genes that may involved in biosurfactant synthesis were not identified. The results showed that using the functional metagenomic to explore metabolic pathways of microbial communities can have great limitation when in the prospection of biosurctants when the operon are larger then 30-40 kb and the genome of wild bacteria contains sparse regulation elements. For the dependent-cultivation approach, two strains that produce biosurfactant were selected: Bacillus safensis CCMA-560 and Gordonia sp. CCMA-559. They were isolated and tested in previous study. The biosurfactant production was opmized through the Placktt-Burman design and the Central Composite Design. The pumilacidin produced by B. safensis CCMA-560 was chemically characterized, and the metabolic pathway that is responsible for its production was genetically characterized. This work was the first report the physiologic, genetic, and chemical analysis on the biosurfactant production by the B. safensis strain Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Melo, Itamar Soares de (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Andreote, Fernando Dini (committee member), Araújo, Welington Luiz de (committee member), Vicentini, Renato (committee member), Duarte, Marta Cristina Teixeira (committee member).

Subjects/Keywords: Biossurfactantes; Metagenômica; Bacillus safensis; Gordonia (Bactéria); Biosurfactants; Metagenomics; Bacillus safensis; Gordonia (Bacteria)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Domingos, Daniela Ferreira, 1. (2014). Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Domingos, Daniela Ferreira, 1984-. “Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.” 2014. Thesis, Universidade Estadual de Campinas. Accessed November 26, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Domingos, Daniela Ferreira, 1984-. “Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.” 2014. Web. 26 Nov 2020.

Vancouver:

Domingos, Daniela Ferreira 1. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2020 Nov 26]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Domingos, Daniela Ferreira 1. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Universidade Estadual de Campinas

2. Fonseca, Francine Souza Alves da, 1979-. Ecologia química de percevejos da família Phloeidae e oxirredutases de Bacillus safensis isolado do petróleo: Phloeidae stink bugs chemical ecology and Bacillus safensis oxidoreductases from petroleum.

Degree: 2013, Universidade Estadual de Campinas

Abstract: Chapter I. This study revealed novel aspects of the chemical ecology of Phloeophana longirostris and Phloea subquadrata from Phloeidae family. This family, has only three genders and four species with characteristic maternal care of eggs and nymphs and camouflage on the trunk of the host tree. The aim of this study was to the identification of major volatile compounds produced by the stink bugs and their relationship with the host trees. The results revealed that after physical stimulation, the insects release aldehydes and unsaturated hydrocarbons, esters and oxoaldehyde as major compounds. These volatile compounds were important in the discrimination of male, female and nymphs of each species. Chapter II. In the second part of this research, we investigated the enzymatic activity of the micro-organism Bacillus safensis isolated from Brazilian oil. This micro-organism has an enzymatic arsenal capable of degrading aromatic organic compounds. The aim of this study was to purify, isolate and identify oxidoreductases present in strain (CFA06) Bacillus safensis. Through this Gram-positive bacterium, highly resistant to ultraviolet radiation, it was possible to identify two oxidoreductases. The novel oxidoreductase, here named BsP315 has 21 kDa and iron and molybdenum bound to its catalytic site, additionally we have isolated a catalase here named BsCat Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Marsaioli, Anita Jocelyne, 1946- (advisor), Universidade Estadual de Campinas. Instituto de Química (institution), Programa de Pós-Graduação em Química (nameofprogram), Ritzel, Andrés Gonzáles (committee member), Vasconcellos, Suzan Pantaroto de (committee member), Miranda, Paulo Cesar Muniz de Lacerda (committee member), Fonseca, Sebastião Ferreira (committee member).

Subjects/Keywords: Ecologia química; Percevejos; Bacillus safensis; Oxirredutase; Chemical ecology; Stink bugs; Bacillus safensis; Oxidoreductase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Fonseca, Francine Souza Alves da, 1. (2013). Ecologia química de percevejos da família Phloeidae e oxirredutases de Bacillus safensis isolado do petróleo: Phloeidae stink bugs chemical ecology and Bacillus safensis oxidoreductases from petroleum. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/249185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Fonseca, Francine Souza Alves da, 1979-. “Ecologia química de percevejos da família Phloeidae e oxirredutases de Bacillus safensis isolado do petróleo: Phloeidae stink bugs chemical ecology and Bacillus safensis oxidoreductases from petroleum.” 2013. Thesis, Universidade Estadual de Campinas. Accessed November 26, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/249185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Fonseca, Francine Souza Alves da, 1979-. “Ecologia química de percevejos da família Phloeidae e oxirredutases de Bacillus safensis isolado do petróleo: Phloeidae stink bugs chemical ecology and Bacillus safensis oxidoreductases from petroleum.” 2013. Web. 26 Nov 2020.

Vancouver:

Fonseca, Francine Souza Alves da 1. Ecologia química de percevejos da família Phloeidae e oxirredutases de Bacillus safensis isolado do petróleo: Phloeidae stink bugs chemical ecology and Bacillus safensis oxidoreductases from petroleum. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2020 Nov 26]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/249185.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fonseca, Francine Souza Alves da 1. Ecologia química de percevejos da família Phloeidae e oxirredutases de Bacillus safensis isolado do petróleo: Phloeidae stink bugs chemical ecology and Bacillus safensis oxidoreductases from petroleum. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/249185

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.