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You searched for subject:(Babesia odocoilei). Showing records 1 – 2 of 2 total matches.

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Texas A&M University

1. Schoelkopf, Lorien. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.

Degree: MS, Veterinary Parasitology, 2005, Texas A&M University

Babesia odocoilei is an intraerythrocytic apicomplexan parasite which infects cervidae, sometimes causing babesiosis. It is vectored by the tick Ixodes scapularis and is distributed throughout the southeastern United States. The geographic and host range continue to extend as new incidence of infection is detected. A genomic DNA region spanning the internal transcribed spacer 1 (ITS1), 5.8S rRNA gene, and ITS2 of ribosomal RNA (rRNA) from 18 B. odocoilei isolates (speciation confirmed by small subunit rRNA analysis) was amplified using the polymerase chain reaction, cloned and sequenced. The isolates originated from 6 different cervidae or bovidae hosts in various U.S. geographic areas. Included in the analysis was a previously described reindeer B. odocoilei-like isolate, RD61, which showed only 99.0% identity in SSU rRNA analysis to B. odocoilei. Percent identity pairwise comparisons among the samples were calculated for both the full ITS1-5.8S-ITS2 and individual genomic regions. Identity values for all comparisons ranged from 90% to 100%, with the exception of RD61, which showed no higher than 88% identity for all gene regions. An analysis of fixed differences identified in the ITS1 and ITS2 gene regions of all clones revealed 21 fixed differences in ITS1, and only 11 in ITS2. Most isolates were found to have 2 overall patterns of fixed differences, although some had 1 or 3. Phylogenetic analysis of all sequences for the entire ITS1-5.8S-ITS2 gene region placed most isolates into 2 distinct groups corresponding to those observed in the analysis of fixed differences. This suggested the presence of at least 2 rRNA transcription units in B. odocoilei. ITS analysis failed to demonstrate host or geographic differences that might serve to pinpoint the source of outbreaks of B. odocoilei in farmed and managed host animals. This failure might result from genetic recombination of ITS genomic regions during the tick vector stage. Lack of conspecificity between the RD61 isolate and B. odocoilei was supported by this study; however, more data are needed to clarify the taxonomic status of this B. odocoilei-like isolate. Advisors/Committee Members: Holman, Patricia J. (advisor), Teel, Pete D. (committee member), Craig, Thomas M. (committee member).

Subjects/Keywords: Babesia odocoilei; Internal transcribed spacers (ITS); Ribosomal RNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Schoelkopf, L. (2005). Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2660

Chicago Manual of Style (16th Edition):

Schoelkopf, Lorien. “Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.” 2005. Masters Thesis, Texas A&M University. Accessed April 11, 2021. http://hdl.handle.net/1969.1/2660.

MLA Handbook (7th Edition):

Schoelkopf, Lorien. “Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.” 2005. Web. 11 Apr 2021.

Vancouver:

Schoelkopf L. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. [Internet] [Masters thesis]. Texas A&M University; 2005. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1969.1/2660.

Council of Science Editors:

Schoelkopf L. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. [Masters Thesis]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2660

2. Milnes, Ellie L. Eco-Epidemiology and Treatment of Babesiosis in Cervids.

Degree: Doctor of Veterinary Science, Department of Pathobiology, 2018, University of Guelph

Babesia odocoilei, a protozoan hemoparasite of white-tailed deer (Odocoileus virginianus) transmitted by Ixodes scapularis ticks, is an increasingly recognized cause of disease in cervids in North America. Following an outbreak of babesiosis in reindeer (Rangifer tarandus tarandus) and wapiti (Cervus canadensis) at the Toronto Zoo in Ontario, Canada, we utilized a prospective postmortem survey to investigate the prevalence of B. odocoilei in wild, farmed, and zoo cervids in Ontario (n=270; 2016-2018) by polymerase chain reaction (PCR) and sequencing of spleen sample extracts. Babesia odocoilei was identified in 1.4% (2/142) of farmed red deer (Cervus elaphus), 4.4% (3/68) of wild white-tailed deer, and 3.4% (1/29) of captive wapiti. Wild white-tailed deer are the candidate wildlife reservoir for B. odocoilei in Ontario. Additionally, we designed a study to investigate the hypothesis that birds can disperse B. odocoilei-infected ticks along migratory flyways. Birds (n = 1,102) were captured during spring migration; the prevalence of I. scapularis infestation was 3.2% in 2016 and 6.7% in 2017, and 0.2% of birds carried one or more I. scapularis ticks that tested PCR-positive for B. odocoilei. Blanket dragging for questing ticks in southern Ontario revealed a minimum infection prevalence for B. odocoilei of up to 4.1% in ticks found in environments used by wild cervids. Babesia odocoilei can cause acute hemolytic crisis in susceptible cervids, thus evidence-based drug treatment protocols are needed to manage the disease. A single intramuscular injection of the anti-protozoal drug imidocarb dipropionate at 3.0 mg/kg may be useful for treatment of cervid babesiosis. To investigate this claim, a pharmacokinetic study of imidocarb was performed in 10 white-tailed deer. Plasma concentrations of imidocarb were determined using high-performance liquid chromatography. The disposition of plasma imidocarb was best characterised by a two-compartment open model, with rapid distribution and slow elimination. The mean  SD maximal imidocarb concentration was 824.92  1.55 ng/mL at 36.47  1.38 minutes post injection. Plasma imidocarb concentrations were comparable to those effective for the treatment of babesiosis in domestic cattle. Clinical efficacy studies are needed to confirm the appropriate dosage regimen in cervids. Advisors/Committee Members: Bienzle, Dorothee (advisor), Nemeth, Nicole (advisor).

Subjects/Keywords: zoological medicine; cervids; deer; babesia; babesia odocoilei; ticks; tick-borne disease; Ixodes scapularis; babesiosis; imidocarb; pharmacokinetics; eco-epidemiology; parasitology; reindeer; wapiti; white-tailed deer

…nuclear 18S rDNA loci used in the identification of Babesia odocoilei from cervid tissue samples… …Ontario, Canada, and tested for Babesia odocoilei infection by PCR using piroplasmspecific… …3 Figure 1.2 Geolocations of documented Babesia odocoilei infections in cervids in the… …16 Figure 1.3 Babesia odocoilei infected erythrocytes in a peripheral blood smear from a… …21 Figure 2.1 Locations of all cervids that underwent postmortem Babesia odocoilei testing… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Milnes, E. L. (2018). Eco-Epidemiology and Treatment of Babesiosis in Cervids. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14265

Chicago Manual of Style (16th Edition):

Milnes, Ellie L. “Eco-Epidemiology and Treatment of Babesiosis in Cervids.” 2018. Doctoral Dissertation, University of Guelph. Accessed April 11, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14265.

MLA Handbook (7th Edition):

Milnes, Ellie L. “Eco-Epidemiology and Treatment of Babesiosis in Cervids.” 2018. Web. 11 Apr 2021.

Vancouver:

Milnes EL. Eco-Epidemiology and Treatment of Babesiosis in Cervids. [Internet] [Doctoral dissertation]. University of Guelph; 2018. [cited 2021 Apr 11]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14265.

Council of Science Editors:

Milnes EL. Eco-Epidemiology and Treatment of Babesiosis in Cervids. [Doctoral Dissertation]. University of Guelph; 2018. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/14265

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