subject:(Babesia microti)

1. Jalovecka, Marie. Établissement du modèle de laboratoire Babesia et ses applications expérimentales : Establishment of Babesia laboratory model and its experimental applications.

Degree: Docteur es, Biologie Santé, 2017, Nantes, Ecole nationale vétérinaire

URL: http://www.theses.fr/2017ONIR101F

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Du fait de l'incidence croissante des infections à Babesia spp., Protozoaire parasite transmis par les tiques, la babésiose est considérée comme maladie émergente en médecine… (more)

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Du fait de l'incidence croissante des infections à Babesia spp., Protozoaire parasite transmis par les tiques, la babésiose est considérée comme maladie émergente en médecine humaine et vétérinaire. Cette thèse apporte un aperçu de la biologie de deux agents principaux de la babésiose humaine, Babesia microti etBabesia divergens. Un modèle quantifié du parasite Babesia est proposé, permettant une étude détaillée de son cycle de vie et l'identification de molécules jouant un rôle dans son acquisition/transmission par le vecteur Ixodes ricinus. Des informations nouvelles et détaillées sur la dissémination de Babesia dans les tissus de la tique sont apportées par les techniques de visualisation/quantification développées. Le développement parasitaire dans les glandes salivaires est particulièrement étudié, car premier site responsable de la transmission du vecteur à l'hôte. Par inactivation ciblée de gènes, nous avons exploré les voies du système immunitaire de la tique, dont les molécules effectrices, et évalué leur rôle dans l'acquisition de Babesia. Une étude détaillée de l'engagement de Babesia dans la phase sexuée est réalisée, en suivant sa cinétique sous l'effet de divers stimuli. De plus, une voie nouvelle de lutte antiparasitaire est proposée envalidant le protéasome de Babesia en tant que cible thérapeutique. D'une façon générale, les travaux présentés élargissent les connaissances actuelles de la biologie du parasite Babesia incluant les interactions moléculaires à l'interface tique-Babesia et contribuent donc au contrôle de la babésiose.

Growing incidence of infections caused by the ticktransmittedprotozoan parasite Babesia spp. definesbabesiosis as an emerging disease from the aspect ofhuman and veterinary medicine. The thesis provides aninsight to biology of two main agents of humanbabesiosis, Babesia microti and Babesia divergens. Weintroduce here the fully optimized quantification model ofBabesia parasite enabling the detailed investigation ofthe parasite developmental cycle and identification ofmolecules playing a role in its acquisition andtransmission by the vector Ixodes ricinus. Novel anddetailed information about Babesia dissemination withinthe tick tissues are given by newly implementedvisualization and quantification techniques. Specialemphasis is paid to parasite development in the ticksalivary glands, the primary site responsible for parasitetransmission from the vector into the host. Using genespecificsilencing we screen the tick immune pathwaysincluding effector molecules and evaluate their role inBabesia acquisition. We also provide a detailed view toBabesia parasite sexual commitment by monitoring itskinetics upon various stimuli. Moreover, a new directionof anti-babesial therapy is proposed by validation of theBabesia proteasome as a drug target. Overall, theresearch presented in the thesis extends the currentknowledge of the Babesia parasite biology includingmolecular interactions at the tick-Babesia interface andthereby could significantly contribute to a potentialcontrol of…

Advisors/Committee Members: Malandrin, Laurence (thesis director).

Subjects/Keywords: Babesia divergens; Babesia microti; Transmission; Tiques; Interactions immunitaires; Voies de signalisation; Engagement sexuel; Proteasome; Babesia divergens; Babesia microti; Transmission model; Tick; Immune interactions; Signalling pathways; Sexual xommitment; Proteasome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jalovecka, M. (2017). Établissement du modèle de laboratoire Babesia et ses applications expérimentales : Establishment of Babesia laboratory model and its experimental applications. (Doctoral Dissertation). Nantes, Ecole nationale vétérinaire. Retrieved from http://www.theses.fr/2017ONIR101F

Chicago Manual of Style (16^th Edition):

Jalovecka, Marie. “Établissement du modèle de laboratoire Babesia et ses applications expérimentales : Establishment of Babesia laboratory model and its experimental applications.” 2017. Doctoral Dissertation, Nantes, Ecole nationale vétérinaire. Accessed April 13, 2021. http://www.theses.fr/2017ONIR101F.

MLA Handbook (7^th Edition):

Jalovecka, Marie. “Établissement du modèle de laboratoire Babesia et ses applications expérimentales : Establishment of Babesia laboratory model and its experimental applications.” 2017. Web. 13 Apr 2021.

Vancouver:

Jalovecka M. Établissement du modèle de laboratoire Babesia et ses applications expérimentales : Establishment of Babesia laboratory model and its experimental applications. [Internet] [Doctoral dissertation]. Nantes, Ecole nationale vétérinaire; 2017. [cited 2021 Apr 13]. Available from: http://www.theses.fr/2017ONIR101F.

Council of Science Editors:

Jalovecka M. Établissement du modèle de laboratoire Babesia et ses applications expérimentales : Establishment of Babesia laboratory model and its experimental applications. [Doctoral Dissertation]. Nantes, Ecole nationale vétérinaire; 2017. Available from: http://www.theses.fr/2017ONIR101F

RMIT University

2. Dunn, J. The mathematical epidemiology of human babesiosis in the north-eastern united states.

Degree: 2014, RMIT University

URL: http://researchbank.rmit.edu.au/view/rmit:160817

► Human babesiosis, spread by the Babesia microti pathogen, is an emerging vector-borne disease transmitted by the Ixodes scapularis tick in the United States. The number… (more)

▼ Human babesiosis, spread by the Babesia microti pathogen, is an emerging vector-borne disease transmitted by the Ixodes scapularis tick in the United States. The number of babesiosis cases has been increasing leading to the classification as an â€œemerging health risk" by the Center for Disease Control. The reasons for emergence remain largely unknown. This thesis presents multi-host, multi-pathogen mechanistic models that explain the zoonotic emergence and persistence of human babesiosis and the Babesia microti pathogen in the United States. A model for the basic reproduction number,R0, and a compartment-type model, combine the seasonal dynamics of tick phenology and within host dynamics in the vertebrate host to identify the key factors that determine emergence and persistence of the Babesia microti pathogen and hence human Babesiosis. It is found that survival of the immature life-stage of the Ixodes scapularis tick, the probability of feeding on the primary vertebrate host (the white-footed mouse, Peromyscus leucopus) and interactions among pathogens, particularly coinfection with Borrelia burgdorferi the causative agent of Lyme disease, in multiply infected hosts can strongly influence emergence. Successful transmission of Babesia microti from one generation of ticks to the next is linked to overlap between the host-seeking activities of the larval and nymphal life-stages of the tick vector, and co-aggregationÂ of these life-stages on white-footed mouse. This contrasts with other vector-borne pathogens where it is typically the abundance of the vector or host, the vector-to-host ratio or the biting rate that are the factors which determine conditions for emergence. The mechanistic models derived in this work are transparent and almost all parameters can be measured directly by laboratory or field studies. Such data are used in this thesis to define ecological conditions for the emergence of Babesia microti and to explore the variation between an island site and a mainland site in the north-eastern United States.

Subjects/Keywords: Fields of Research; Basic reproduction number; Compartmental model; Global sensitivity analysis; Babesia microti; Lyme disease; Co-aggregation; Coinfection; Emergence

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APA (6^th Edition):

Dunn, J. (2014). The mathematical epidemiology of human babesiosis in the north-eastern united states. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:160817

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dunn, J. “The mathematical epidemiology of human babesiosis in the north-eastern united states.” 2014. Thesis, RMIT University. Accessed April 13, 2021. http://researchbank.rmit.edu.au/view/rmit:160817.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dunn, J. “The mathematical epidemiology of human babesiosis in the north-eastern united states.” 2014. Web. 13 Apr 2021.

Vancouver:

Dunn J. The mathematical epidemiology of human babesiosis in the north-eastern united states. [Internet] [Thesis]. RMIT University; 2014. [cited 2021 Apr 13]. Available from: http://researchbank.rmit.edu.au/view/rmit:160817.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dunn J. The mathematical epidemiology of human babesiosis in the north-eastern united states. [Thesis]. RMIT University; 2014. Available from: http://researchbank.rmit.edu.au/view/rmit:160817

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

3. Oliveira, Mauro R. Babesia microti : host parasite interactios and tick transmission.

Degree: PhD, Graduate School, 1979, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487084652612316

Subjects/Keywords: Biology; Ticks as carriers of disease; Babesia microti

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, M. R. (1979). Babesia microti : host parasite interactios and tick transmission. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487084652612316

Chicago Manual of Style (16^th Edition):

Oliveira, Mauro R. “Babesia microti : host parasite interactios and tick transmission.” 1979. Doctoral Dissertation, The Ohio State University. Accessed April 13, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487084652612316.

MLA Handbook (7^th Edition):

Oliveira, Mauro R. “Babesia microti : host parasite interactios and tick transmission.” 1979. Web. 13 Apr 2021.

Vancouver:

Oliveira MR. Babesia microti : host parasite interactios and tick transmission. [Internet] [Doctoral dissertation]. The Ohio State University; 1979. [cited 2021 Apr 13]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487084652612316.

Council of Science Editors:

Oliveira MR. Babesia microti : host parasite interactios and tick transmission. [Doctoral Dissertation]. The Ohio State University; 1979. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487084652612316

4. A. DELLA PEPA. VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/219128

► LEISHMANIA INFANTUM INFECTION IN STRAY CATS IN A NON-ENDEMIC AREA IN NORTHERN ITALY E. Spada, DVM, PhD, Researcher 1, A. Della Pepa, DVM 1, A.… (more)

▼ LEISHMANIA INFANTUM INFECTION IN STRAY CATS IN A NON-ENDEMIC AREA IN NORTHERN ITALY E. Spada, DVM, PhD, Researcher 1, A. Della Pepa, DVM 1, A. Migliazzo, DVM, PhD 2, G. Bagnagatti De Giorgi, DVM 1, R. Perego, DVM, PhD 1, D. Proverbio, DVM, PhD, Professor 1 1Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Universita degli Studi di Milano, Milan, Italy 2Centro di Referenza Nazionale per le Leishmaniosi,Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy Tipologia: Ricerca Originale Area di interesse: Medicina interna Purpose of the work. To assess the prevalence of leishmaniosis in a large representative sample of stray cats from this non-endemic area, and to analyze the results according to clinical, laboratory and infectious data 2. MOLECULAR STUDY ON VECTOR-BORNE INFECTIONS IN URBAN STRAY COLONY CAT IN NORTHENRN ITALY Eva Spada§, DVM, PhD, Researcher Daniela Proverbio§, DVM, PhD, Professor Alessandra Della Pepa§, DVM Paola Galluzzo*, Biologist Roberta Perego§, DVM, PhD Giada Bagnagatti De Giorgi§, DVM Abstract Feline vector-borne diseases are caused by a wide range of pathogens, which are transmitted by arthropods. Many of these infections have zoonotic implications and feral cats may potentially act as sentinels of human and pet health. The present study investigated the prevalence of vector-borne infections in feral colony cats in the city of Milan in northern Italy. Blood samples from 260 feral cats were evaluated, with conventional PCR, for the presence of DNA associated with hemoplasmas (Mycoplasma haemofelis and Mycoplasma haemominutum), Rickettsia spp., Anaplasma phagocytophilum, Ehrlichia spp. and Babesia microti. Odd ratios (OR) were calculated to identify risk factors for infection with vector-borne pathogens. Positive PCR was found in 156 out of 260 subjects (60%), with a prevalence of 33.1% for hemoplasmas, 31.9% for Rickettsia spp., 17.7% for A. phagocytophilum , 6.7% for Ehrlichia spp. (out of 30 samples), and 1.2% for B. microti spp (out of 168 samples). Statistical analysis revealed a correlation between infections with Rickettsia spp. and hemoplasmas (OR=1.95, P=0.02). Additionally, Rickettsia spp. infection was associated with ocular infection (OR=2.21, P=0.02). We conclude that vector-borne infections, including zoonotic diseases, are present in feral cats of Milan. Thus, domestic cats exposed to the outdoors should be routinely monitored and treated for ectoparasites to minimize disease onset and potential transmission of zoonotic agents to humans. Moreover, as these vector-borne infections are transmitted through blood, feline blood donors from this area should be screened by PCR. Advisors/Committee Members: tutor: D. Proverbio, correlator: E. Spada, coordinator: F. Cremonesi, PROVERBIO, DANIELA, CREMONESI, FAUSTO.

Subjects/Keywords: feral cats; northern Italy; PCR; vector-borne infection; Mycoplasma haemofelis; Mycoplasma haemominutum; haemoplasmas; Rickettsia spp.; Anaplasma phagocytophilum; Ehrlichia spp.; Babesia microti; Leishmania infantum; Settore MED/09 - Medicina Interna

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

PEPA, A. D. (2013). VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/219128

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

PEPA, A. DELLA. “VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY.” 2013. Thesis, Università degli Studi di Milano. Accessed April 13, 2021. http://hdl.handle.net/2434/219128.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

PEPA, A. DELLA. “VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY.” 2013. Web. 13 Apr 2021.

Vancouver:

PEPA AD. VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2434/219128.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

PEPA AD. VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/219128

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Baradji, Issa. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.

Degree: PhD, Veterinary Microbiology, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

► Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected… (more)

▼ Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected to play a role in erythrocyte invasion. To investigate interaction between AMA-1 and the host cell, the ectodomain region of the B. microti ama-1 gene was cloned into an expression vector, expressed as a histidine-tagged fusion protein, and used to probe red blood cell membrane proteins in far Western blot assays. The B. microti ama-1 ectodomain, which excludes the signal peptide and the transmembrane region of the open reading frame, was amplified from a cloned gene sequence. The AMA-1 ectodomain is a membrane bound polypeptide that extends into the extracellular space and is most likely to interact or initiate interaction with the host red blood cell surface receptor(s). The amplicon was ligated into a protein expression vector to produce a 58.1 kDa recombinant His-tagged fusion protein, which was confirmed by Western blot analysis. The recombinant B. microti AMA-1 fusion protein was enriched on nickel affinity columns and then used to probe mouse, human and horse red blood cell membrane proteins in far Western blot assays. Babesia microti AMA-1 consistently reacted strongly with a protein migrating at 49 kDa. A similar reaction occurred between the B. microti AMA-1 and horse red blood cell membrane proteins, suggesting that similar interacting proteins of this size are shared by red blood cells from the three species. The B. microti AMA-1 may bind to red blood cell membrane sialic-acid groups, as shown for other Babesia spp. This may explain the signal at the 49 kDa position observed between B. microti AMA-1 and red blood cell membrane proteins from three different species. Further studies may determine if the binding epitopes of the red blood cell binding partner at this position vary and contribute to the specificity of each parasite AMA-1 for their respective host cells. Advisors/Committee Members: Holman, Patricia J. (advisor), Ball, Judith M. (committee member), Magill, Clint C. (committee member), Womack, James E. (committee member).

Subjects/Keywords: Babesia microti; Apical Membrane Antigen-1; AMA-1; erythrocyte; parasite ligand; red blood cell; receptor; protein-protein interaction

…viii NOMENCLATURE AMA-1 Apical Membrane Antigen –1 BmAMA-1 Babesia microti Apical… …Membrane Antigen-1 B. microti ama-1 gene Babesia microti ama-1 gene BmAMA-1 protein Babesia… …19 Results �� …... �� .. 24 IV BABESIA MICROTI AMA-1 RECOMBINANT PROTEIN ��. 27… …microti in mouse blood �� ….. 7 Figure 2. Babesia microti AMA-1 full gene sequence… …with predicted amino acid residues. �� ….… 20 Figure 3. Babesia microti…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baradji, I. (2010). The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

Chicago Manual of Style (16^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

MLA Handbook (7^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Web. 13 Apr 2021.

Vancouver:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

Council of Science Editors:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

6. Westwood, Mary Lynn. Infection Prevalence in a Novel Ixodes scapularis Population in Northern Wisconsin.

Degree: MS, Biological Sciences, 2017, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1503765696276339

► Ixodes scapularis (i.e. the blacklegged or deer tick) is an important vector of emerging human pathogens. Over the past few decades, the incidence of blacklegged… (more)

Subjects/Keywords: Biology; Ecology; Epidemiology; Public Health; Ixodes scapularis; ticks; diseases; Anaplasma phagocytophilum; Babesia microti; blacklegged tick; deer tick; vector; Ixodes; Anaplasma; Babesia; Borrelia burgdorferi; Borrelia; disease ecology; vector; vector-borne; zoonoses; zoonotic disease; Lyme

…x28;e.g.#Borrelia#burgdorferi,#Anaplasma#phagocytophilum,#and#Babesia#microti)# are#not… …2011F2013).## # BABESIA#MICROTI# # Babesiosis#is#caused#by#the#protozoan#blood#parasite… …Babesia#microti.#Though# many#individuals#infected#with#B.#microti#remain#asymptomatic,#certain… …Transmission# studies#of#Babesia#microti#in#Ixodes#ricinus#ticks#and#gerbils.#Journal#of#Clinical… …R.S.,#Keesing,#F.,#2012.#Reservoir# Competence#of#Wildlife#Host#Species#for#Babesia#microti…

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APA (6^th Edition):

Westwood, M. L. (2017). Infection Prevalence in a Novel Ixodes scapularis Population in Northern Wisconsin. (Masters Thesis). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1503765696276339

Chicago Manual of Style (16^th Edition):

Westwood, Mary Lynn. “Infection Prevalence in a Novel Ixodes scapularis Population in Northern Wisconsin.” 2017. Masters Thesis, Wright State University. Accessed April 13, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wright1503765696276339.

MLA Handbook (7^th Edition):

Westwood, Mary Lynn. “Infection Prevalence in a Novel Ixodes scapularis Population in Northern Wisconsin.” 2017. Web. 13 Apr 2021.

Vancouver:

Westwood ML. Infection Prevalence in a Novel Ixodes scapularis Population in Northern Wisconsin. [Internet] [Masters thesis]. Wright State University; 2017. [cited 2021 Apr 13]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1503765696276339.

Council of Science Editors:

Westwood ML. Infection Prevalence in a Novel Ixodes scapularis Population in Northern Wisconsin. [Masters Thesis]. Wright State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1503765696276339

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