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You searched for subject:(BSA filtration). Showing records 1 – 3 of 3 total matches.

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McMaster University

1. Kazemi, Amir Sadegh. Development of stirred well filtration as a high-throughput technique for downstream bioprocessing.

Degree: MASc, 2014, McMaster University

Micro-scale processing (MSP) techniques are miniaturized version of upstream and downstream conventional unit operations that are designed to accelerate the pace of bioprocess design and development. Previous ‘dead end’ filtration studies have demonstrated the usefulness of this concept for membrane filtration processes. However, these experiments were performed without stirring which is the most common strategy to control the effects of concentration polarization and fouling on filtration performance. In this work, the pressure-driven stirred conditions of a conventional stirred-cell module were integrated with a 96-well filter plate to develop a high throughput technique called ‘stirred-well filtration’ (SWF). The design allowed for up to eight constant flux filtration experiments to be conducted at once using a multi-rack programmable syringe pump and a magnetic lateral tumble stirrer. An array of pressure transducers was used to monitor the transmembrane pressure (TMP) in each well. The protein sieving behavior and fouling propensity of Omega™ ultrafiltration membranes were assessed via a combination of hydraulic permeability measurements and protein sieving tests in constant filtrate flux mode. The TMP profile during filtration of bovine serum albumin (BSA) solution was strongly dependent on the stirring conditions – for example the maximum TMP in the stirred wells were an average of 7.5, 3.8, and 2.6 times lower than those in the unstirred wells at filtrate fluxes of 12, 36, and 60 LMH (5, 15, and 25 μL/min) respectively. The consistency of the data across different wells for the same stirring condition was very good. To demonstrate the effectiveness of the SWF technique, the eight tests for a simple 22 factorial design-of-experiments (DOE) test with duplicates was run to evaluate the effect of solution pH and salt concentration on protein filtration. The combination of SWF with statistical methods such as DOE is shown to be an effective strategy for high-throughput optimization of membrane filtration processes.

Dissertation

Master of Applied Science (MASc)

Advisors/Committee Members: Latulippe, David, Chemical Engineering.

Subjects/Keywords: Microscale processing; High-throughput testing; Downstream bioprocessing; Stirred well filtration (SWF); BSA filtration; Micromixing; Fouling test; Omega™ membrane

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kazemi, A. S. (2014). Development of stirred well filtration as a high-throughput technique for downstream bioprocessing. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/16153

Chicago Manual of Style (16th Edition):

Kazemi, Amir Sadegh. “Development of stirred well filtration as a high-throughput technique for downstream bioprocessing.” 2014. Masters Thesis, McMaster University. Accessed March 01, 2021. http://hdl.handle.net/11375/16153.

MLA Handbook (7th Edition):

Kazemi, Amir Sadegh. “Development of stirred well filtration as a high-throughput technique for downstream bioprocessing.” 2014. Web. 01 Mar 2021.

Vancouver:

Kazemi AS. Development of stirred well filtration as a high-throughput technique for downstream bioprocessing. [Internet] [Masters thesis]. McMaster University; 2014. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/11375/16153.

Council of Science Editors:

Kazemi AS. Development of stirred well filtration as a high-throughput technique for downstream bioprocessing. [Masters Thesis]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16153


Edith Cowan University

2. Trivedi, Samarth. Vertically-aligned carbon nanotube membranes for water desalination and ultrafiltration.

Degree: 2017, Edith Cowan University

Even though the earth is 70% covered with water, there is a severe shortage of fresh water. Modern water filtration techniques includes Reverse Osmosis (RO), Forward Osmosis (FO), and Solar Distillation etc. Due to its high separation performance and comparatively low prices, membrane technology is widely applied in many fields such as medical science, environmental science and many more. Despite its benefits such as energy efficiency and high performance, they are susceptible to fouling. Such drawbacks encourage the invention of new type of membranes. There are many research groups who are working on different membrane materials, surface modification or incorporation of nanoparticles in existing membranes. Types of nanomaterials include nanoparticles (quantum dots), different polymers or novel carbon structures like Carbon Nanotube (CNTs) or Graphene. CNTs doped in polymer is the best choice for fabrication of these novel membranes. A combination of the CNTs and hydrophobic polymer is the best combination for the mixed matrix class of membrane fabrication. But dispersion of CNTs in polymer to achieve best results is the biggest challenge. The first phase of the research undertaken throughout the PhD project involved the development of a novel membrane fabrication process, where various densities of Vertically Aligned Carbon Nanotubes (VACNTs) were developed, and, through a spin coating process, spaces between adjacent VACNTs were filled with Poly(dimethylsiloxane) commonly known as PDMS. A prepared mixed matrix block was sliced into 25μm thick slices using the microtome machine. Sliced membranes were transferred onto PVDF support membranes which acted as support layer only. The prepared membranes were tested for water flux and salt rejection capabilities. The performance of VACNT membranes of densities 5×109, 1010, 5×1010 and 1011 cm-2 were 918, 1008, 1112 and 1202 LMH at 1 bar respectively. The permeance of 1202 LMH was around 30 times higher than traditional Polyethersulfone (PES) membrane. The salt rejection was initially 99% but later on it was reduced and stabilized at 96% due to concentration polarization in used setup of modified dead-end filtration cell. Experimental results confirmed that the permeability of VACNT membranes increases with the density of the VACNTs, while the salt rejection is almost independent of the VACNT density. The second phase of the PhD project focused on membrane fabrication and characterization. The prepared membranes were first tested for different polar and non-polar liquids. Later on those membranes were treated with dry Reactive Ion Etching (RIE) and their surfaces were modified. Through dry RIE, the membrane surfaces were made more hydrophilic compare to the original hydrophobic surface. This hydrophilicity was increased due to hydroxyl ion addition on the membrane surfaces. The filtration of polar and non-polar liquids was again measured. The hydrophilicity gave the advantage of selectivity of polar or non-polar liquids through specific membranes. Experimental results, which…

Subjects/Keywords: Vertically aligned Carbon Nanotubes; water filtration; PDMS; BSA; biofouling; fouling; RO membrane; Ultra-filtration; Physical Sciences and Mathematics; Physics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Trivedi, S. (2017). Vertically-aligned carbon nanotube membranes for water desalination and ultrafiltration. (Thesis). Edith Cowan University. Retrieved from https://ro.ecu.edu.au/theses/2035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Trivedi, Samarth. “Vertically-aligned carbon nanotube membranes for water desalination and ultrafiltration.” 2017. Thesis, Edith Cowan University. Accessed March 01, 2021. https://ro.ecu.edu.au/theses/2035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Trivedi, Samarth. “Vertically-aligned carbon nanotube membranes for water desalination and ultrafiltration.” 2017. Web. 01 Mar 2021.

Vancouver:

Trivedi S. Vertically-aligned carbon nanotube membranes for water desalination and ultrafiltration. [Internet] [Thesis]. Edith Cowan University; 2017. [cited 2021 Mar 01]. Available from: https://ro.ecu.edu.au/theses/2035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Trivedi S. Vertically-aligned carbon nanotube membranes for water desalination and ultrafiltration. [Thesis]. Edith Cowan University; 2017. Available from: https://ro.ecu.edu.au/theses/2035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Kazemi, Amir Sadegh. Development of High-throughput Membrane Filtration Techniques for Biological and Environmental Applications.

Degree: PhD, 2018, McMaster University

Membrane filtration processes are widely utilized across different industrial sectors for biological and environmental separations. Examples of the former are sterile filtration and protein fractionation via microfiltration (MF) and ultrafiltration (UF) while drinking water treatment, tertiary treatment of wastewater, water reuse and desalination via MF, UF, nanofiltration (NF) and reverse-osmosis (RO) are examples of the latter. A common misconception is that the performance of membrane separation is solely dependent on the membrane pore size, whereas a multitude of parameters including solution conditions, solute concentration, presence of specific ions, hydrodynamic conditions, membrane structure and surface properties can significantly influence the separation performance and the membrane’s fouling propensity. The conventional approach for studying filtration performance is to use a single lab- or pilot-scale module and perform numerous experiments in a sequential manner which is both time-consuming and requires large amounts of material. Alternatively, high-throughput (HT) techniques, defined as the miniaturized version of conventional unit operations which allow for multiple experiments to be run in parallel and require a small amount of sample, can be employed. There is a growing interest in the use of HT techniques to speed up the testing and optimization of membrane-based separations. In this work, different HT screening approaches are developed and utilized for the evaluation and optimization of filtration performance using flat-sheet and hollow-fiber (HF) membranes used in biological and environmental separations. The effects of various process factors were evaluated on the separation of different biomolecules by combining a HT filtration method using flat-sheet UF membranes and design-of-experiments methods. Additionally, a novel HT platform was introduced for multi-modal (constant transmembrane pressure vs. constant flux) testing of flat-sheet membranes used in bio-separations. Furthermore, the first-ever HT modules for parallel testing of HF membranes were developed for rapid fouling tests as well as extended filtration evaluation experiments. The usefulness of the modules was demonstrated by evaluating the filtration performance of different foulants under various operating conditions as well as running surface modification experiments. The techniques described herein can be employed for rapid determination of the optimal combination of conditions that result in the best filtration performance for different membrane separation applications and thus eliminate the need to perform numerous conventional lab-scale tests. Overall, more than 250 filtration tests and 350 hydraulic permeability measurements were performed and analyzed using the HT platforms developed in this thesis.

Thesis

Doctor of Philosophy (PhD)

Membrane filtration is widely used as a key separation process in different industries. For example, microfiltration (MF) and ultrafiltration (UF) are used for sterilization and…

Advisors/Committee Members: Latulippe, David, Chemical Engineering.

Subjects/Keywords: Membrane filtration; Ultrafiltration; Downstream bio-processing; High-throughput (HT) testing; Wastewater treatment; Hollow-fiber membranes; Humic acids; High-throughput filtration; Design-of-experiments (DOE); Process optimization; Microscale filtration; Microfluidic flow control system; Stirred well filtration; SWF; High-throughput hollow-fiber module; HT-HF; Constant TMP; Constant flux; Multi-modal filtration; Bioseparation; MMFC; Microscale parallel-structured, cross-flow filtration; MS-PS-CFF; PEG; Dextran; FITC-Dextran; BSA; DNA; IgG; α-lactalbumin; Biomolecule separation; Module hydrodynamics; Concentration polarization; Membrane fouling; Micromixing; Omega™ membrane; Microscale processing; Fouling test; PVDF membrane; Surface modification; Polydopamine; Membrane cleaning; Membrane backwashing; Sodium alginate; Polyethersulfone; PES; Hydraulic permeability; Membrane permeability; ZeeWeed® membrane; Filtration ionic strength; Filtration pH; Solution conditions; Water treatment; Environmental separations; Biological separations

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kazemi, A. S. (2018). Development of High-throughput Membrane Filtration Techniques for Biological and Environmental Applications. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/23404

Chicago Manual of Style (16th Edition):

Kazemi, Amir Sadegh. “Development of High-throughput Membrane Filtration Techniques for Biological and Environmental Applications.” 2018. Doctoral Dissertation, McMaster University. Accessed March 01, 2021. http://hdl.handle.net/11375/23404.

MLA Handbook (7th Edition):

Kazemi, Amir Sadegh. “Development of High-throughput Membrane Filtration Techniques for Biological and Environmental Applications.” 2018. Web. 01 Mar 2021.

Vancouver:

Kazemi AS. Development of High-throughput Membrane Filtration Techniques for Biological and Environmental Applications. [Internet] [Doctoral dissertation]. McMaster University; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/11375/23404.

Council of Science Editors:

Kazemi AS. Development of High-throughput Membrane Filtration Techniques for Biological and Environmental Applications. [Doctoral Dissertation]. McMaster University; 2018. Available from: http://hdl.handle.net/11375/23404

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