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You searched for subject:(Azidouridine). Showing records 1 – 2 of 2 total matches.

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Northeastern University

1. Suresh, Swapna. Synthesis of protected 5'-aminouridine for modification of solid-support in synthesis of modified siRNA.

Degree: MS, Department of Chemistry and Chemical Biology, 2008, Northeastern University

My research was focused on the multi-step synthesis of solid support bearing 5'-aminouridine for automated synthesis of siRNAs. The project started with the preparation of 5'-azidouridine using either a Mitsonobu reaction with hydrazoic acid or a reaction of uridine with lithium azide, triphenylphosphine and carbon tetrabromide. Azide was hydrogenated to form 5'-aminouridine and further treated with para-methoxytrityl chloride to protect the amino group. The generated 5'-amino-5'-N-methoxytrityluridine was then treated with a solution of ortho-chlorobenzoyl chloride to protect the 2' hydroxyl group, followed by succinic anhydride in the presence of 4-dimethylaminopyridine (DMAP) to form a mixture of protected 2' and 3' succinates. All new compounds were analyzed and characterized using NMR techniques. The protected amino uridine succinate was then loaded onto solid support - long chain aminoalkyl controlled pore glass (LCAA-CPG) to be used in solid phase synthesis of amide modified siRNA. The succinate moiety was coupled to the free amino groups on the CPG using N,N'-dicyclohexylcarbodiimide (DCC ) N-hydroxybenzotriazole (HOBT ) to generate a stable amide bond between the ribonucleoside and the CPG. The loading of the protected 5'-aminouridine on CPG was quantified by following acid catalyzed detritylation at 478 nm using a UV-VIS spectrometer. The solid phase support will be used for preparation of amide modified siRNAs. We propose that these non-ionic mimics of the phosphate backbone will confer high nuclease resistance to siRNAs. Further, the backbone of the modified siRNAs will have an overall decreased negative charge, which may help crossing the cellular membrane. We anticipate that these properties may improve the biodistribution and pharmacokinetics of modified siRNAs.

Subjects/Keywords: Organic chemistry; 5'-azidouridine; siRNAs; Bearings (Machinery); Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Suresh, S. (2008). Synthesis of protected 5'-aminouridine for modification of solid-support in synthesis of modified siRNA. (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/d10016119

Chicago Manual of Style (16th Edition):

Suresh, Swapna. “Synthesis of protected 5'-aminouridine for modification of solid-support in synthesis of modified siRNA.” 2008. Masters Thesis, Northeastern University. Accessed April 10, 2021. http://hdl.handle.net/2047/d10016119.

MLA Handbook (7th Edition):

Suresh, Swapna. “Synthesis of protected 5'-aminouridine for modification of solid-support in synthesis of modified siRNA.” 2008. Web. 10 Apr 2021.

Vancouver:

Suresh S. Synthesis of protected 5'-aminouridine for modification of solid-support in synthesis of modified siRNA. [Internet] [Masters thesis]. Northeastern University; 2008. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/2047/d10016119.

Council of Science Editors:

Suresh S. Synthesis of protected 5'-aminouridine for modification of solid-support in synthesis of modified siRNA. [Masters Thesis]. Northeastern University; 2008. Available from: http://hdl.handle.net/2047/d10016119


University of Georgia

2. Clark, Teresa Nicole. Quantitative analysis and pharmacokinetics of reverse transcriptase inhibitors in the pregnant rat.

Degree: 2014, University of Georgia

For over two decades there has been a ceaseless search for more effective treatments of HIV/AIDS. Today there are a number of different therapies that fall into one of three categories, based on their mechanism of action. All currently marketed anti-HIV drugs are classified as either 1) nucleoside reverse transcriptase inhibitors, 2) non-nucleoside reverse transcriptase inhibitors, or 3) protease inhibitors. Each of these compounds has gone through FDA-regulated clinical trials to prove safety and efficacy. Due to a number of reasons, pregnant women are generally not used during clinical trials, so very little is known about the behavior of drugs during pregnancy. A pregnant rat model has been developed to investigate the pharmacokinetics and placental transport of drugs during pregnancy. Presented here are validated analytical methods for the extraction and quantitation of the nucleoside reverse transcriptase inhibitors azidouridine, didanosine and abacavir in the various matrices needed for maternal-fetal pharmacokinetic studies. Also presented here are the pharmacokinetics of two of these compounds, azidouridine (as compared to zidovudine) and abacavir, using a pregnant rat model.

Subjects/Keywords: Azidouridine; AZDU; Zidovudine; AZT; Abacavir; ABC; Didanosine; DDI; High Performance Liquid Chromatography; HPLC-UV; LC/MS/MS; Pharmacokinetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Clark, T. N. (2014). Quantitative analysis and pharmacokinetics of reverse transcriptase inhibitors in the pregnant rat. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/21009

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Clark, Teresa Nicole. “Quantitative analysis and pharmacokinetics of reverse transcriptase inhibitors in the pregnant rat.” 2014. Thesis, University of Georgia. Accessed April 10, 2021. http://hdl.handle.net/10724/21009.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Clark, Teresa Nicole. “Quantitative analysis and pharmacokinetics of reverse transcriptase inhibitors in the pregnant rat.” 2014. Web. 10 Apr 2021.

Vancouver:

Clark TN. Quantitative analysis and pharmacokinetics of reverse transcriptase inhibitors in the pregnant rat. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10724/21009.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Clark TN. Quantitative analysis and pharmacokinetics of reverse transcriptase inhibitors in the pregnant rat. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/21009

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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