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Freie Universität Berlin

1. Ebert, Marco Günter. Studies of the prevalence in eye swabs and the tissue- and cells tropism, particularly in cases of equine Keratokonjunktivitis.

Degree: 2007, Freie Universität Berlin

The aim of this project was to further investigate the etiopathogenetic role of EHV-2. The main goal was to exam the clinical relevance of EHV-2 in the genesis of conjunctivitis and keratitis, tissue tropism and possible sites of latency in horses. Blood samples and swabs from 28 eye diseased test group animals and 28 healthy control group horses were taken twice within 4-5 weeks in the horse clinic of the Tierärztliche Hochschule Hanover. These samples were virologically investigated in the Institute of Virology, Faculty of Veterinary Medicine, Freie Universität Berlin. Furthermore, post-mortem tissues from 20 naturally infected horses were analysed for EHV-2. EHV-2 was detected by various serological methods, nested PCR (nPCR) and co-cultivation in both the test and control group. A statistically significant correlation between infection and tested groups was not observed. Interestingly, EHV-2 was detected in mules using the aforementioned methods, implicating that mules appear to be permissive for EHV-2. The sampling of the eye was carried out in parallel with cotton wool swaps and Cytobrushes from the conjunctival fossa. In both groups EHV-2 was detected significantly more often in the samples obtained by the Cytobrush. As a Cytobrush sample contains much more cell material than a cotton wool swab sample, it is likely that the higher frequency of EHV-2 detection in Cytobrush samples indicates an intracellular virus load of the deeper stratums of the conjunctiva. Therefore, the conjunctiva might be a site of latency of EHV-2. The fact that EHV-2 was detected more frequently in Cytobrush samples from the conjunctival fossa than in PBMC, an already known site of latency, provides further evidence that the conjunctiva may be a site of latency. In post-mortem tissues, EHV-2 was found more often in mucous membrane (56% of the conjunctiva, 2/2 noses) than in the lachrymal glands (31%), the lung (24%), nerve tissue (20% of the trigeminal ganglia, 10% of the optic nerves) and the aqueous fluid (0%) via nPCR. These results demonstrate that EHV-2 was detected most frequently in the conjunctiva of post-mortem tissues. In tissues analysed from the lachrymal gland, nose and optic nerve, EHV-2 was present only in conjunction with areas which were observed to have an EHV-2-positive conjunctiva. This suggests that the conjunctiva might act as a portal for EHV-2, and that the virus spreads from the conjunctiva to other tissues. Another indicator for virus spread via the lacrimal-nose-channel was indicated by the fact that 75% of horses with EHV-2-positive nose swabs were also positive for EHV-2 in the eye swab sample. EHV-2 was detected in a naturally-intrauterine-infected foetus, which was aborted during the eighth month of pregnancy. EHV-2 was detected in the trigeminal ganglia of this foetus. Genetically differing EHV-2 serovars were present in both swabs and in blood samples taken from the same horse simultaneously as well as in eye- and nose- and blood-samples taken from one animal at various time points. No genetic… Advisors/Committee Members: n (gender), Priv.-Doz. Dr. K. Borchers (firstReferee), Univ.-Prof. Dr. A. Sterner-Kock (furtherReferee), Univ.-Prof. Dr. H. Weiß (furtherReferee).

Subjects/Keywords: Horse diseases; eye-diseases; keratoconjunctivitis-; tropisms; equine-herpesvirus; Pferdekrankheiten; Augenkrankheiten; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ebert, M. G. (2007). Studies of the prevalence in eye swabs and the tissue- and cells tropism, particularly in cases of equine Keratokonjunktivitis. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/11930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ebert, Marco Günter. “Studies of the prevalence in eye swabs and the tissue- and cells tropism, particularly in cases of equine Keratokonjunktivitis.” 2007. Thesis, Freie Universität Berlin. Accessed August 13, 2020. https://refubium.fu-berlin.de/handle/fub188/11930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ebert, Marco Günter. “Studies of the prevalence in eye swabs and the tissue- and cells tropism, particularly in cases of equine Keratokonjunktivitis.” 2007. Web. 13 Aug 2020.

Vancouver:

Ebert MG. Studies of the prevalence in eye swabs and the tissue- and cells tropism, particularly in cases of equine Keratokonjunktivitis. [Internet] [Thesis]. Freie Universität Berlin; 2007. [cited 2020 Aug 13]. Available from: https://refubium.fu-berlin.de/handle/fub188/11930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ebert MG. Studies of the prevalence in eye swabs and the tissue- and cells tropism, particularly in cases of equine Keratokonjunktivitis. [Thesis]. Freie Universität Berlin; 2007. Available from: https://refubium.fu-berlin.de/handle/fub188/11930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


ETH Zürich

2. Bergeles, Christos. Visually servoing wireless magnetic intraocular microrobots.

Degree: 2011, ETH Zürich

Subjects/Keywords: MIKROROBOTER; MEDIZINISCHE ROBOTER (MEDIZINISCHE TECHNIK); OPERATIONSABLAUF + OPERATIONSTECHNIK (CHIRURGIE); COMPUTERVISION (KÜNSTLICHE INTELLIGENZ); AUGENHEILKUNDE + AUGENKRANKHEITEN (MEDIZIN); OPTISCHE MESSGERÄTE FÜR MEDIZIN UND AUGENOPTIK (OPTISCHE INSTRUMENTE); ROBOTERSTEUERUNG + ROBOTERREGELUNG; MICRO ROBOTS; MEDICAL ROBOTS (MEDICAL ENGINEERING); OPERATIVE PROCEDURE + OPERATIVE TECHNIQUES (SURGERY); COMPUTER VISION + SCENE UNDERSTANDING (ARTIFICIAL INTELLIGENCE); OPHTHALMOLOGY + EYE DISEASES (MEDICINE); MEASURING INSTRUMENTS FOR MEDICAL AND OPHTALMIC OPTICS (OPTICAL INSTRUMENTS); ROBOT CONTROL; info:eu-repo/classification/ddc/621.3; info:eu-repo/classification/ddc/610; Electric engineering; Medical sciences, medicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bergeles, C. (2011). Visually servoing wireless magnetic intraocular microrobots. (Doctoral Dissertation). ETH Zürich. Retrieved from http://hdl.handle.net/20.500.11850/152595

Chicago Manual of Style (16th Edition):

Bergeles, Christos. “Visually servoing wireless magnetic intraocular microrobots.” 2011. Doctoral Dissertation, ETH Zürich. Accessed August 13, 2020. http://hdl.handle.net/20.500.11850/152595.

MLA Handbook (7th Edition):

Bergeles, Christos. “Visually servoing wireless magnetic intraocular microrobots.” 2011. Web. 13 Aug 2020.

Vancouver:

Bergeles C. Visually servoing wireless magnetic intraocular microrobots. [Internet] [Doctoral dissertation]. ETH Zürich; 2011. [cited 2020 Aug 13]. Available from: http://hdl.handle.net/20.500.11850/152595.

Council of Science Editors:

Bergeles C. Visually servoing wireless magnetic intraocular microrobots. [Doctoral Dissertation]. ETH Zürich; 2011. Available from: http://hdl.handle.net/20.500.11850/152595

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