subject:(Aspergillus)

University of Debrecen

1. Borsos, Krisztina. Az Aspergillus nidulans és az Aspergillus rugulosus amilolitikus aktivitásának összehasonlítása .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/108089

► A keményítő az egyik legnagyobb mennyiségben keletkező (és egyben az ember által legnagyobb mennyiségben előállított) poliszacharid a Földön. Keményítőből kiindulva biokonverziós úton számos olyan anyagot… (more)

▼ A keményítő az egyik legnagyobb mennyiségben keletkező (és egyben az ember által legnagyobb mennyiségben előállított) poliszacharid a Földön. Keményítőből kiindulva biokonverziós úton számos olyan anyagot (pl. maltodextrin, kukorica szirup, maltóz szirup, fruktóz szirup, trehalóz, ciklodextrinek, etanol) állítanak elő, melyet az élelmiszeripar de akár a kozmetikai- és gyógyszeripar is nagy mennyiségben felhasznál. Ezen alapanyagok előállítása rendszerint keményítőbontó (amilolitikus) enzimek segítségével történik (Machovic és Janecek 2007, Synowiecki 2007, Plou és munkatársai 2007). A vízoldható keményítő, illetve a különféle keményítő hidrolizátumok a fermentációs iparban is igen elterjedtek, mint olcsó, gyors növekedést és gyenge karbon repressziót biztosító szénforrások (Abe és munkatársai 2008, Braaksma és munkatársai 2008). Az Aspergillus fajok keményítőbontó képességének vizsgálata így több ok miatt is érdekes lehet: Egyfelől a keményítő biokonverziójában használható új enzimek azonosítását teheti lehetővé (jelenleg is számos Aspergillus-eredetű amilolitikus enzimet állítanak elő és használnak az iparban; Machovic és Janecek 2007, Synowiecki 2007, Plou és munkatársai 2007), de segítheti a fermentációs paraméterek optimalizálását, illetve a törzsnemesítési stratégiák kidolgozását is az Aspergillus fajokat használó fermentációs folyamatokban (pl. lovasztatin, citromsav, glükóz oxidáz, hidrolitikus enzimek gyártása, Geiser és LoBuglio 2001). Aspergillus fajok gyakran izolálhatóak a talajból és elhalt növényi részeket tartalmazó mintákból, ahol a növényi sejtfalpoliszacharidok (pl. cellulóz, hemicellulóz, pektin anyagok) bontása mellett a növényi keményítő hidrolízisében és hasznosításában is részt vesznek (Geiser és LoBuglio 2001). Az Aspergillusok keményítőhasznosításának vizsgálatából ezért reálisabb képet kaphatunk e gombák természetes élőhelyén mutatott viselkedéséről, mint a „klasszikus” glükóz tartalmú táptalajokon végzett kísérletek esetében. Szakdolgozatom részeként az alább kísérleteket végeztem el: - Az Aspergillus nidulans és az A. rugulosus növekedésének vizsgálata keményítő, glükóz és különféle -glükozidokat tartalmazó táptalajok felületén. - Keményítő szénforráson növesztett tenyészetekből származó fermentlevek amilolitikus aktivitásának jellemzése, összehasonlítása. - Távolabbi céljaink között szerepel a két faj keményítő hasznosításában résztvevő enzimeinek, transzportereinek azonosítása is. E munka keretében, RT-PCR-es vizsgálatokkal keményítő jelenlétében indukálódó glükóz transzporter géneket azonosítottunk. Advisors/Committee Members: Tóth, Viktória (advisor).

Subjects/Keywords: Aspergillus nidulans; Aspergillus rugulosus; gombák

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APA (6^th Edition):

Borsos, K. (2011). Az Aspergillus nidulans és az Aspergillus rugulosus amilolitikus aktivitásának összehasonlítása . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/108089

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Borsos, Krisztina. “Az Aspergillus nidulans és az Aspergillus rugulosus amilolitikus aktivitásának összehasonlítása .” 2011. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/108089.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Borsos, Krisztina. “Az Aspergillus nidulans és az Aspergillus rugulosus amilolitikus aktivitásának összehasonlítása .” 2011. Web. 03 Mar 2021.

Vancouver:

Borsos K. Az Aspergillus nidulans és az Aspergillus rugulosus amilolitikus aktivitásának összehasonlítása . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/108089.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Borsos K. Az Aspergillus nidulans és az Aspergillus rugulosus amilolitikus aktivitásának összehasonlítása . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/108089

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Koda, Akio. Development of efficient protein expression system in filamentous fungi, Aspergillus species : Aspergillus属糸状菌における異種タンパク質の効率的生産系の開発; Aspergillus ゾクシジョウキンニオケルイシュタンパクシツノコウリツテキセイサンケイノカイハツ.

Degree: Nara Institute of Science and Technology / 奈良先端科学技術大学院大学

URL: http://hdl.handle.net/10061/4150

Subjects/Keywords: Aspergillus

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APA (6^th Edition):

Koda, A. (n.d.). Development of efficient protein expression system in filamentous fungi, Aspergillus species : Aspergillus属糸状菌における異種タンパク質の効率的生産系の開発; Aspergillus ゾクシジョウキンニオケルイシュタンパクシツノコウリツテキセイサンケイノカイハツ. (Thesis). Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10061/4150

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Koda, Akio. “Development of efficient protein expression system in filamentous fungi, Aspergillus species : Aspergillus属糸状菌における異種タンパク質の効率的生産系の開発; Aspergillus ゾクシジョウキンニオケルイシュタンパクシツノコウリツテキセイサンケイノカイハツ.” Thesis, Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Accessed March 03, 2021. http://hdl.handle.net/10061/4150.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Koda, Akio. “Development of efficient protein expression system in filamentous fungi, Aspergillus species : Aspergillus属糸状菌における異種タンパク質の効率的生産系の開発; Aspergillus ゾクシジョウキンニオケルイシュタンパクシツノコウリツテキセイサンケイノカイハツ.” Web. 03 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Koda A. Development of efficient protein expression system in filamentous fungi, Aspergillus species : Aspergillus属糸状菌における異種タンパク質の効率的生産系の開発; Aspergillus ゾクシジョウキンニオケルイシュタンパクシツノコウリツテキセイサンケイノカイハツ. [Internet] [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10061/4150.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Koda A. Development of efficient protein expression system in filamentous fungi, Aspergillus species : Aspergillus属糸状菌における異種タンパク質の効率的生産系の開発; Aspergillus ゾクシジョウキンニオケルイシュタンパクシツノコウリツテキセイサンケイノカイハツ. [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; Available from: http://hdl.handle.net/10061/4150

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Oregon State University

3. Baker, Gerald Merritt. The parasite Aspergillus flavus Link and other fungi of the biosphere of the alkali bee Nomia melanderi Ckll., in eastern Oregon and Washington.

Degree: PhD, Botany and Plant Pathology, 1969, Oregon State University

URL: http://hdl.handle.net/1957/46343

► The pathogenicity of A. flavus on alkali bee larvae was studied using sample materials from three nesting sites of these soil inhabiting bees along the… (more)

▼ The pathogenicity of A. flavus on alkali bee larvae was studied using sample materials from three nesting sites of these soil inhabiting bees along the Oregon-Washington state line. Soil saprophytes of the biosphere of the alkali bee were also studied. Field observations and direction of soil cores from bee beds provided a basis for estimates of A. flavus disease at the three sites. Fungi, including A. flavus and soil saprophytes were isolated from: soil samples, the surface of adult alkali bees, pollen balls, and larval feces. In vitro disease induction experiments were carried out comparing uninoculated larvae and larvae surface inoculated with conidia of A. flavus. The tests were conducted at five levels of relative humidity within each of three temperature treatments. Field observations and soil cores revealed A. flavus as the pre-dominante fungus pathogen in two of the three sites studied. One site was free of the pathogen. Studies of soil dilution plates and fungal isolations from adult bees, pollen balls, and larval feces disclosed propagules of A. flavus in the biosphere of the bees in two of the three beds. Saprophytic fungi of the biosphere did not vary significantly over a three year period. These fungi provided a useful index of soil contamination of adult bees, pollen balls, and larval feces. Studies of the effect of relative humidity and temperature on disease induction in larvae surface inoculated with A. flavus conidia revealed that temperatures of 25° to 30°C and relative-humidities from 90 to 100 percent favored disease development while lower temperatures and/or lower humidities inhibited disease induction. The increased incidence of A. flavus disease of larvae during the spring and summer is considered to result from increasing soil temperatures and relative humidities within the brood cells, Conversely, a reduced incidence of disease during the fall and winter results from lower temperatures. Higher incidence of A. flavus disease in one of the beds (the Garbe bed) was attributed to its age and higher bee population. Soil cores taken from the Garbe bed to establish the Harris bed account for the prevalence of disease in the latter. The lack of A. flavus disease in the Wallace-Key bed was not clear, but its younger age and lower population density offer a partial explanation. Control of A. flavus within the nesting site might logically aim at elimination of the fungus from the soil. Sub-irrigation of the beds with fungicide is one possible approach. Advisors/Committee Members: Roth, L. F. (advisor).

Subjects/Keywords: Aspergillus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baker, G. M. (1969). The parasite Aspergillus flavus Link and other fungi of the biosphere of the alkali bee Nomia melanderi Ckll., in eastern Oregon and Washington. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46343

Chicago Manual of Style (16^th Edition):

Baker, Gerald Merritt. “The parasite Aspergillus flavus Link and other fungi of the biosphere of the alkali bee Nomia melanderi Ckll., in eastern Oregon and Washington.” 1969. Doctoral Dissertation, Oregon State University. Accessed March 03, 2021. http://hdl.handle.net/1957/46343.

MLA Handbook (7^th Edition):

Baker, Gerald Merritt. “The parasite Aspergillus flavus Link and other fungi of the biosphere of the alkali bee Nomia melanderi Ckll., in eastern Oregon and Washington.” 1969. Web. 03 Mar 2021.

Vancouver:

Baker GM. The parasite Aspergillus flavus Link and other fungi of the biosphere of the alkali bee Nomia melanderi Ckll., in eastern Oregon and Washington. [Internet] [Doctoral dissertation]. Oregon State University; 1969. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1957/46343.

Council of Science Editors:

Baker GM. The parasite Aspergillus flavus Link and other fungi of the biosphere of the alkali bee Nomia melanderi Ckll., in eastern Oregon and Washington. [Doctoral Dissertation]. Oregon State University; 1969. Available from: http://hdl.handle.net/1957/46343

Colorado State University

4. Finefield, Jennifer M. Studies on the biosynthesis of prenylated indole secondary metabolites from Aspergillus versicolor and Aspergillus sp.; and A novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin: Novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin, A.

Degree: PhD, Chemistry, 2011, Colorado State University

URL: http://hdl.handle.net/10217/46212

► Herein are documented our efforts in two projects, beginning with studies toward elucidating the biosynthesis of prenylated indole alkaloids from two different Aspergillus species. Marine-derived… (more)

Subjects/Keywords: naphthyridine; Aspergillus

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APA (6^th Edition):

Finefield, J. M. (2011). Studies on the biosynthesis of prenylated indole secondary metabolites from Aspergillus versicolor and Aspergillus sp.; and A novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin: Novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin, A. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/46212

Chicago Manual of Style (16^th Edition):

Finefield, Jennifer M. “Studies on the biosynthesis of prenylated indole secondary metabolites from Aspergillus versicolor and Aspergillus sp.; and A novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin: Novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin, A.” 2011. Doctoral Dissertation, Colorado State University. Accessed March 03, 2021. http://hdl.handle.net/10217/46212.

MLA Handbook (7^th Edition):

Finefield, Jennifer M. “Studies on the biosynthesis of prenylated indole secondary metabolites from Aspergillus versicolor and Aspergillus sp.; and A novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin: Novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin, A.” 2011. Web. 03 Mar 2021.

Vancouver:

Finefield JM. Studies on the biosynthesis of prenylated indole secondary metabolites from Aspergillus versicolor and Aspergillus sp.; and A novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin: Novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin, A. [Internet] [Doctoral dissertation]. Colorado State University; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10217/46212.

Council of Science Editors:

Finefield JM. Studies on the biosynthesis of prenylated indole secondary metabolites from Aspergillus versicolor and Aspergillus sp.; and A novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin: Novel approach to tumor specific drug delivery: use of a naphthyridine drug linker with a DNA hairpin, A. [Doctoral Dissertation]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/46212

University of Debrecen

5. Farkas, Anna. Két PCR eljárás összehasonlítása az Aspergillus-fertőzések korai kimutatására magas kockázatú betegeknél .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/106168

► Az orvostudomány fejlődése az utóbbi évtizedekben jelentősen hozzájárult ahhoz, hogy új kezelési stratégiák és eljárások jöjjenek létre. Ezek azonban új problémákat is hoznak magukkal. Így… (more)

Subjects/Keywords: Aspergillus; PCR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Farkas, A. (2011). Két PCR eljárás összehasonlítása az Aspergillus-fertőzések korai kimutatására magas kockázatú betegeknél . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/106168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Farkas, Anna. “Két PCR eljárás összehasonlítása az Aspergillus-fertőzések korai kimutatására magas kockázatú betegeknél .” 2011. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/106168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Farkas, Anna. “Két PCR eljárás összehasonlítása az Aspergillus-fertőzések korai kimutatására magas kockázatú betegeknél .” 2011. Web. 03 Mar 2021.

Vancouver:

Farkas A. Két PCR eljárás összehasonlítása az Aspergillus-fertőzések korai kimutatására magas kockázatú betegeknél . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/106168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Farkas A. Két PCR eljárás összehasonlítása az Aspergillus-fertőzések korai kimutatására magas kockázatú betegeknél . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/106168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

6. Kónya, Gábor. Feltételezett réz és kadmium iontranszporter vizsgálata Aspergillus deléciós mutáns törzsekben .

Degree: DE – Természettudományi és Technológiai Kar – Biotechnológiai Intézet, University of Debrecen

URL: http://hdl.handle.net/2437/267348

Subjects/Keywords: Aspergillus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kónya, G. (n.d.). Feltételezett réz és kadmium iontranszporter vizsgálata Aspergillus deléciós mutáns törzsekben . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/267348

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kónya, Gábor. “Feltételezett réz és kadmium iontranszporter vizsgálata Aspergillus deléciós mutáns törzsekben .” Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/267348.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kónya, Gábor. “Feltételezett réz és kadmium iontranszporter vizsgálata Aspergillus deléciós mutáns törzsekben .” Web. 03 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Kónya G. Feltételezett réz és kadmium iontranszporter vizsgálata Aspergillus deléciós mutáns törzsekben . [Internet] [Thesis]. University of Debrecen; [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/267348.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Kónya G. Feltételezett réz és kadmium iontranszporter vizsgálata Aspergillus deléciós mutáns törzsekben . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/267348

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Debrecen

7. Bakó, Anett. Fonalas gombák közötti interakció vizsgálata acetát jelenlétében .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2013, University of Debrecen

URL: http://hdl.handle.net/2437/177650

Munkánk során két gombatelep között fellépő interakciókat vizsgáltunk, ezen belül azt, hogy hogyan hatnak egymás növekedésére. Kísérleteinkhez Aspergillus nidulans és Aspergillus niger fajokat használtuk fel különböző szénforrásokon tenyésztve. Kerestük az antifungális hatás okait. Advisors/Committee Members: Szilágyi-Bónizs, Melinda (advisor).

Subjects/Keywords: autolízis; Aspergillus

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APA (6^th Edition):

Bakó, A. (2013). Fonalas gombák közötti interakció vizsgálata acetát jelenlétében . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/177650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bakó, Anett. “Fonalas gombák közötti interakció vizsgálata acetát jelenlétében .” 2013. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/177650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bakó, Anett. “Fonalas gombák közötti interakció vizsgálata acetát jelenlétében .” 2013. Web. 03 Mar 2021.

Vancouver:

Bakó A. Fonalas gombák közötti interakció vizsgálata acetát jelenlétében . [Internet] [Thesis]. University of Debrecen; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/177650.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bakó A. Fonalas gombák közötti interakció vizsgálata acetát jelenlétében . [Thesis]. University of Debrecen; 2013. Available from: http://hdl.handle.net/2437/177650

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

8. Ondecs, Ádám. Aspergillus nidulans fonalas gomba NADPH termelésének manipulálása és élettani következményei .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2013, University of Debrecen

URL: http://hdl.handle.net/2437/177681

Aspergillus nidulans gombák NADPH túlteremléséhez szükséges törzsek előállítása, és ehhez szükséges molekuláris biológiai módszerek ismertetése. Advisors/Committee Members: Fekete, Erzsébet (advisor).

Subjects/Keywords: Aspergillus; NADPH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ondecs, �. (2013). Aspergillus nidulans fonalas gomba NADPH termelésének manipulálása és élettani következményei . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/177681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ondecs, Ádám. “Aspergillus nidulans fonalas gomba NADPH termelésének manipulálása és élettani következményei .” 2013. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/177681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ondecs, Ádám. “Aspergillus nidulans fonalas gomba NADPH termelésének manipulálása és élettani következményei .” 2013. Web. 03 Mar 2021.

Vancouver:

Ondecs �. Aspergillus nidulans fonalas gomba NADPH termelésének manipulálása és élettani következményei . [Internet] [Thesis]. University of Debrecen; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/177681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ondecs �. Aspergillus nidulans fonalas gomba NADPH termelésének manipulálása és élettani következményei . [Thesis]. University of Debrecen; 2013. Available from: http://hdl.handle.net/2437/177681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

9. Rákosi, Annamária. Aspergillus fajok nehézfém toleranciájának a tanulmányozása .

Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2014, University of Debrecen

URL: http://hdl.handle.net/2437/190226

Szakdolgozatomban az Aspergillus fajok nehézfém toleranciáját tanulmányoztam. Az Aspergillus fajok toleranciájáról egyre több adat áll rendelkezésünkre, melynek segítségével további kutatások indíthatóak el, amelyek hozzásegítenek a nehézfém tolerancia molekuláris hátterének mélyebb megértéséhez. Advisors/Committee Members: Pócsi, István (advisor).

Subjects/Keywords: aspergillus; nehézfém

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rákosi, A. (2014). Aspergillus fajok nehézfém toleranciájának a tanulmányozása . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/190226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rákosi, Annamária. “Aspergillus fajok nehézfém toleranciájának a tanulmányozása .” 2014. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/190226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rákosi, Annamária. “Aspergillus fajok nehézfém toleranciájának a tanulmányozása .” 2014. Web. 03 Mar 2021.

Vancouver:

Rákosi A. Aspergillus fajok nehézfém toleranciájának a tanulmányozása . [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/190226.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rákosi A. Aspergillus fajok nehézfém toleranciájának a tanulmányozása . [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/190226

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

10. Szaniszló, Szilvia. Automata DNS izoláló módszer érzékenységének vizsgálata és szerepe az Aspergillus fumigatus diagnosztikájában .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2012, University of Debrecen

URL: http://hdl.handle.net/2437/129774

Az Aspergillus nemzetségbe tartozó gombák környezetünkben mindenhol megtalálhatóak, de humán opportunista patogénként, általában csak legyengült immunrendszerű egyéneket képesek megfertőzni. Azonban az Aspergillus fumigatus okozta invazív tüdőaspergillosis kimutatása az aspecifikus tünetek miatt nagyon nehéz. Az Aspergillosis diagnosztikájára kifejlesztett módszerkombináció érzékenység és specificitás vizsgálata. Advisors/Committee Members: Biró, Sándor (advisor).

Subjects/Keywords: Aspergillus; Aspergillosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Szaniszló, S. (2012). Automata DNS izoláló módszer érzékenységének vizsgálata és szerepe az Aspergillus fumigatus diagnosztikájában . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/129774

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Szaniszló, Szilvia. “Automata DNS izoláló módszer érzékenységének vizsgálata és szerepe az Aspergillus fumigatus diagnosztikájában .” 2012. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/129774.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Szaniszló, Szilvia. “Automata DNS izoláló módszer érzékenységének vizsgálata és szerepe az Aspergillus fumigatus diagnosztikájában .” 2012. Web. 03 Mar 2021.

Vancouver:

Szaniszló S. Automata DNS izoláló módszer érzékenységének vizsgálata és szerepe az Aspergillus fumigatus diagnosztikájában . [Internet] [Thesis]. University of Debrecen; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/129774.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Szaniszló S. Automata DNS izoláló módszer érzékenységének vizsgálata és szerepe az Aspergillus fumigatus diagnosztikájában . [Thesis]. University of Debrecen; 2012. Available from: http://hdl.handle.net/2437/129774

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

11. Kassakürti, Zsanett. Aspergillus fumigatus ellen ható antifungális szerek hatékonyságának növelése sziderofórokkal .

Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, University of Debrecen

URL: http://hdl.handle.net/2437/227224

Aspergillus fumigatus ellen ható antifungális szerek. Advisors/Committee Members: Pócsi, István (advisor).

Subjects/Keywords: Aspergillus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kassakürti, Z. (n.d.). Aspergillus fumigatus ellen ható antifungális szerek hatékonyságának növelése sziderofórokkal . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/227224

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kassakürti, Zsanett. “Aspergillus fumigatus ellen ható antifungális szerek hatékonyságának növelése sziderofórokkal .” Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/227224.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kassakürti, Zsanett. “Aspergillus fumigatus ellen ható antifungális szerek hatékonyságának növelése sziderofórokkal .” Web. 03 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Kassakürti Z. Aspergillus fumigatus ellen ható antifungális szerek hatékonyságának növelése sziderofórokkal . [Internet] [Thesis]. University of Debrecen; [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/227224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Kassakürti Z. Aspergillus fumigatus ellen ható antifungális szerek hatékonyságának növelése sziderofórokkal . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/227224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Debrecen

12. Maczonkai, Adrienn. Szikes talajok gombaközösségének vizsgálata .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2013, University of Debrecen

URL: http://hdl.handle.net/2437/169251

► A tervezett vizsgálataink célja a hortobágyi szikes területek (azaz nagy Na+ tartalmú és lúgos pH-jú habitatok) mikroszkópikus gombavilágának előzetes tanulmányozása volt egy későbbi részletesebb vizsgálatsorozat… (more)

Subjects/Keywords: Aspergillus; szikes

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APA (6^th Edition):

Maczonkai, A. (2013). Szikes talajok gombaközösségének vizsgálata . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/169251

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maczonkai, Adrienn. “Szikes talajok gombaközösségének vizsgálata .” 2013. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/169251.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maczonkai, Adrienn. “Szikes talajok gombaközösségének vizsgálata .” 2013. Web. 03 Mar 2021.

Vancouver:

Maczonkai A. Szikes talajok gombaközösségének vizsgálata . [Internet] [Thesis]. University of Debrecen; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/169251.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maczonkai A. Szikes talajok gombaközösségének vizsgálata . [Thesis]. University of Debrecen; 2013. Available from: http://hdl.handle.net/2437/169251

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

13. Némethy, Fruzsina. Aspergillus nidulans és Aspergillus niger fonalas gombák galaktóz fenotípusainak analízise .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2010, University of Debrecen

URL: http://hdl.handle.net/2437/95626

► Munkánk során arra a kérdésre kerestük a választ, hogy betudható-e a galaktokináz rendszer defektusának az Aspergillus niger D-galaktóz negatív fenotípusa, vagyis az a tény, hogy… (more)

Subjects/Keywords: Aspergillus; galaktóz

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APA (6^th Edition):

Némethy, F. (2010). Aspergillus nidulans és Aspergillus niger fonalas gombák galaktóz fenotípusainak analízise . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/95626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Némethy, Fruzsina. “Aspergillus nidulans és Aspergillus niger fonalas gombák galaktóz fenotípusainak analízise .” 2010. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/95626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Némethy, Fruzsina. “Aspergillus nidulans és Aspergillus niger fonalas gombák galaktóz fenotípusainak analízise .” 2010. Web. 03 Mar 2021.

Vancouver:

Némethy F. Aspergillus nidulans és Aspergillus niger fonalas gombák galaktóz fenotípusainak analízise . [Internet] [Thesis]. University of Debrecen; 2010. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/95626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Némethy F. Aspergillus nidulans és Aspergillus niger fonalas gombák galaktóz fenotípusainak analízise . [Thesis]. University of Debrecen; 2010. Available from: http://hdl.handle.net/2437/95626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

14. Talbot, Jessica Jane. Common and cryptic Aspergillus species – one health pathogens .

Degree: 2018, University of Sydney

URL: http://hdl.handle.net/2123/19930

► Fungal Aspergillus species cause invasive and chronic disease in humans and other animals. This thesis investigated cryptic and common Aspergillus species in A. section Fumigati;… (more)

▼ Fungal Aspergillus species cause invasive and chronic disease in humans and other animals. This thesis investigated cryptic and common Aspergillus species in A. section Fumigati; their prevalence in Australia and virulence factors impacting the efficacy of the most commonly used antifungal drugs, triazoles. An environmental (soil and air) investigation of 104 Aspergillus isolates for cryptic A. sect. Fumigati species, focusing on the A. viridinutans species complex (AVSC), found a moderate risk of exposure to known pathogenic and cryptic species, but low risk AVSC exposure in indoor and outdoor domestic environments. A new AVSC species was discovered, A. frankstonensis (morphology; ITS region, BenA, CalM, MCM7, actin and RPB2 gene sequencing; antifungal susceptibility; and extrolite analysis are described). Sequencing (ITS, BenA) of clinical isolates from captive birds (n=30) identified A. fumigatus as the most common cause of disease and A. restrictus as pathogenic. Triazole resistance amongst A. fumigatus isolates from clinical and environmental samples is documented over two studies, identifying a low prevalence in Australia. The first investigated pathogenic A. fumigatus isolates from dogs and cats (n=50), finding triazole resistance in one Australian isolate from a dog in the early 1990’s (Sensititre™ YeastOne™YO8). The second investigated clinical (148 human, 21 veterinary) and environmental (n=185) Australian isolates (VIPcheck™ and Sensititre™ YeastOne™ YO10), confirming triazole resistance in three human origin isolates, associated with cyp51A mutations TR34/L98H and G54R. Triazole susceptibility (CLSI) of 37 AVSC isolates were investigated, detecting high triazole minimum inhibitory concentrations (84% of isolates). Compared to wild-type A. fumigatus, high rates of cyp51A mutations were detected on sequencing; however protein homology modelling did not confer resistance. This research informs our understanding of A. sect. Fumigati pathogens in Australia.

Subjects/Keywords: Aspergillus; aspergillosis; Aspergillus frankstonensis; azole resistance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Talbot, J. J. (2018). Common and cryptic Aspergillus species – one health pathogens . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/19930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Talbot, Jessica Jane. “Common and cryptic Aspergillus species – one health pathogens .” 2018. Thesis, University of Sydney. Accessed March 03, 2021. http://hdl.handle.net/2123/19930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Talbot, Jessica Jane. “Common and cryptic Aspergillus species – one health pathogens .” 2018. Web. 03 Mar 2021.

Vancouver:

Talbot JJ. Common and cryptic Aspergillus species – one health pathogens . [Internet] [Thesis]. University of Sydney; 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2123/19930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Talbot JJ. Common and cryptic Aspergillus species – one health pathogens . [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/19930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

15. Asters, Matthew Constantine. Characterization of maize genes in response to Aspergillus flavus infection and aflatoxin accumulation.

Degree: MS, Biochemistry, Molecular Biology, Entomology and Plant Pathology, 2014, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-07292014-214702/ ;

► Aspergillus flavus is a pathogenic fungus causing maize ear rot disease and producing aflatoxins that are carcinogenic to humans and animals. Characterizing maize host… (more)

Subjects/Keywords: Aspergillus flavus; Aflatoxin

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APA (6^th Edition):

Asters, M. C. (2014). Characterization of maize genes in response to Aspergillus flavus infection and aflatoxin accumulation. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-07292014-214702/ ;

Chicago Manual of Style (16^th Edition):

Asters, Matthew Constantine. “Characterization of maize genes in response to Aspergillus flavus infection and aflatoxin accumulation.” 2014. Masters Thesis, Mississippi State University. Accessed March 03, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-07292014-214702/ ;.

MLA Handbook (7^th Edition):

Asters, Matthew Constantine. “Characterization of maize genes in response to Aspergillus flavus infection and aflatoxin accumulation.” 2014. Web. 03 Mar 2021.

Vancouver:

Asters MC. Characterization of maize genes in response to Aspergillus flavus infection and aflatoxin accumulation. [Internet] [Masters thesis]. Mississippi State University; 2014. [cited 2021 Mar 03]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-07292014-214702/ ;.

Council of Science Editors:

Asters MC. Characterization of maize genes in response to Aspergillus flavus infection and aflatoxin accumulation. [Masters Thesis]. Mississippi State University; 2014. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-07292014-214702/ ;

Rhodes University

16. Pindura, Mitchell Kingsley Chido. The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger.

Degree: Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2012, Rhodes University

URL: http://hdl.handle.net/10962/d1018267

► Fructooligosaccharides (FOS) are short-chain fructans with a terminal glucose moiety and are found naturally in many plant species. Besides their wide use as an alternative… (more)

▼ Fructooligosaccharides (FOS) are short-chain fructans with a terminal glucose moiety and are found naturally in many plant species. Besides their wide use as an alternative sweetener in food and beverage industry, FOS have shown great potential as neutraceuticals against diabetes, colon cancer and bowel disease. The uses of FOS are dependent on the degree of polymerisation that they exhibit. β-fructofuranosidase (FFase) and fructosyltransferase (FTase) enzymes are capable of synthesing FOS from carbohydrate raw materials such as chicory and sugar beet. The aim of this study was to investigate the synthesis of FOS of a pre-defined chain length, from sucrose, by the enzyme FopAp; a β-fructofuranosidase from Aspergillus niger. ATCC 20611. The crude enzyme FopAp was successfully purified, with a yield of 78.20 %, by ammonium sulphate precipitation and anion exchange chromatography. Two protein fractions, named FA and FB were shown to exhibit FFase activity. SDS PAGE analysis revealed two proteins with molecular weights of 112 kDa and 78 kDa, which were identified as a FFase and a hydrolase. Temperature and pH optima of 20 ºC and 9, respectively, were observed for the transfructosylation activity in the FFase. The purified FFase exhibited a half life of 1.5 hrs under optimal conditions. Substrate kinetic studies indicated a high hydrolytic activity at low sucrose concentrations, with Vmax and Km of 1.25 μmol/ml/min and 3.28 mM, respectively. Analysis by response surface methodology identified temperature and pH to be significant factors for the production of kestose and nystose, at a 95 % level of confidence. These findings were confirmed by neural networks constructed to identify optimal conditions of FOS synthesis.FOS synthesis was found to be optimal between pH 6 and pH 9 at 25 ºC. The factor of reaction time was found to be insignificant within the selected experimental constraints, for both FOS species. The findings of this investigation are very important as the foundations of a commercially viable synthetic process for the production of FOS.

Subjects/Keywords: Aspergillus niger; Oligosaccharides

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APA (6^th Edition):

Pindura, M. K. C. (2012). The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1018267

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pindura, Mitchell Kingsley Chido. “The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger.” 2012. Thesis, Rhodes University. Accessed March 03, 2021. http://hdl.handle.net/10962/d1018267.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pindura, Mitchell Kingsley Chido. “The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger.” 2012. Web. 03 Mar 2021.

Vancouver:

Pindura MKC. The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger. [Internet] [Thesis]. Rhodes University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10962/d1018267.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pindura MKC. The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger. [Thesis]. Rhodes University; 2012. Available from: http://hdl.handle.net/10962/d1018267

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

17. Dias, Fernanda Furlan Gonçalves, 1986-. Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica: Production, biochemical characterization and application of fungal L-asparaginase.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322120

► Abstract: L-asparaginases are enzymes that catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. Microbial L-asparaginases have received attention due to its role in… (more)

▼ Abstract: L-asparaginases are enzymes that catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. Microbial L-asparaginases have received attention due to its role in the treatment of acute lymphoblastic leukemia and due to its increasing use in the food industry aimed at reducing acrylamide formation during food processing. An increasing number of studies has made the application of this enzyme in different food matrices and proven its effects on the reduction of acrylamide. In this study, the production of L-asparaginase by eight strains of Aspergillus fungi was assessed. Among the eight tested strains, A. niger LBA 02, A. oryzae CCT 3940 and A. oryzae LBA 01 showed higher production of L-asparaginase, 26,0 U mL-1; 20,6 U mL-1 e 19,1 U mL-1, respectively. The strain of A. oryzae CCT 3940 was selected for the production of L-asparaginase studies considering the biochemical characteristics of the enzyme (stability at pH 6-8 and 40-50 ° C, and no inhibition in the presence of NaCl) for potential application in food. A sequential optimization strategy was used to determine the effect of different carbon sources, nitrogen sources, inductor concentration (L-asparagine), inoculum concentration, initial pH of the medium, temperature and agitation in the production of L-asparaginase from A. oryzae CCT 3940 in submerged fermentation. In the fermentation of the microorganism at 30 °C, in modified Czapeck medium consisting of proline (2%), glucose (0.5%), L-asparagine (0.2%), yeast extract (0.5%), KH2PO4 (0.152%), KCl (0.052%), MgSO4.7H2O (0.052%), CuNO3.3H2O (0.0001%), ZnSO4.7H2O (0.0001%) and FeSO4.7H2O (0,0001%) pH adjusted to pH 8.0, inoculum concentration of 3 × 107 spores mL-1, 72 hours of fermentation at 150 rpm, L-asparaginase activity was 67.49 U mL-1. This represents an increase of 225% compared to non-optimized initial conditions. The L-asparaginase from A. oryzae CCT 3940 was purified in a four-step process, and the molecular mass was estimated as 115 kDa. The purified L-asparaginase from A. oryzae CCT 3940 exhibited optimal activity at pH 7.0-8.0 at 40-50 °C. The purified enzyme showed anti-proliferative activity against various human tumor cell lines without, however, completely inhibit the growth of non-tumor cell line. In order to verify the effectiveness of the enzyme and of the commercial preparation of L-asparaginase on reducing acrylamide in foods, potato cubes (2 cm edge) were subjected to soaking for 30 minutes at 50 °C in three treatments: (i) distilled water; (ii) distilled water with purified L-asparaginase and (iii) distilled water with commercial enzyme. The purified L-asparaginase promoted a 72% reduction in acrylamide formation whereas the commercial enzyme showed 92% reduction when compared to the control system. The results obtained in this study indicate that L-asparaginase enzyme from A. oryzae CCT 3940 has attractive characteristics and interesting biological activity for the food industry and the pharmaceutical industries Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Sato, Helia Harumi, 1952- (advisor), Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos (institution), Programa de Pós-Graduação em Ciência de Alimentos (nameofprogram), Arisseto, Adriana Pavesi (committee member), Macedo, Juliana Alves (committee member), Santos, Luciana Ferracini dos (committee member), Alencar, Severino Matias (committee member).

Subjects/Keywords: Acrilamida; Aspergillus oryzae; Aspergillus niger; L-asparaginase; L-asparaginase; Acrylamide; Aspergillus oryzae; Aspergillus niger

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APA (6^th Edition):

Dias, Fernanda Furlan Gonçalves, 1. (2016). Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica: Production, biochemical characterization and application of fungal L-asparaginase. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/322120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dias, Fernanda Furlan Gonçalves, 1986-. “Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica: Production, biochemical characterization and application of fungal L-asparaginase.” 2016. Thesis, Universidade Estadual de Campinas. Accessed March 03, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/322120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dias, Fernanda Furlan Gonçalves, 1986-. “Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica: Production, biochemical characterization and application of fungal L-asparaginase.” 2016. Web. 03 Mar 2021.

Vancouver:

Dias, Fernanda Furlan Gonçalves 1. Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica: Production, biochemical characterization and application of fungal L-asparaginase. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Mar 03]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dias, Fernanda Furlan Gonçalves 1. Produção, caracterização bioquímica e aplicação de L-asparaginase fúngica: Production, biochemical characterization and application of fungal L-asparaginase. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. MORAES, Heloiza Maria da Silva Oliveira. Quantificação de melanina e gliotoxina e diversidade genética de isolados clínicos e ambientais de Aspergillus .

Degree: 2015, Universidade Federal de Pernambuco

URL: https://repositorio.ufpe.br/handle/123456789/26598

► Espécies de Aspergillus são sapróbias presentes no solo ou matéria vegetal em decomposição e dependendo do estado imunológico do hospedeiro, podem causar infecções fúngicas oportunistas,… (more)

▼ Espécies de Aspergillus são sapróbias presentes no solo ou matéria vegetal em decomposição e dependendo do estado imunológico do hospedeiro, podem causar infecções fúngicas oportunistas, como aspergilose invasiva. Os objetivos deste trabalho foram analisar a variabilidade genética, quantificar melanina e gliotoxina em 57 isolados de A. fumigatus, A. flavus e A. niger de origens ambiental e clínica e estabelecer um modelo experimental de aspergilose pulmonar. Através do uso de marcadores moleculares ISSR, foi avaliada a variabilidade genética entre os isolados. Os isolados de A. flavus, com o uso dos marcadores (GACA)₄ e (GTG)₅, apresentaram agrupamentos em função da origem. O marcador (GTG)₅ foi o que melhor separou os isolados em função da origem clínica ou ambiental, enquanto o marcador (GACA)₄ foi o que mostrou melhor a variabilidade genética entre os isolados. Os conídios de todos os isolados produziram partículas de melanina, a partir de uma sequência de tratamentos com enzimas, agentes desnaturantes e ácido concentrado em alta temperatura, não havendo diferença estatística em função da espécie e do substrato. A análise dos espectros de infravermelho do pigmento produzido por cada isolado mostrou diferenças entre os perfis de melanina. As partículas escuras foram visualizadas em microscopia óptica e eletrônica de varredura. Oito isolados de A. fumigatus produziram gliotoxina nas condições de cultivo e análise estabelecidas, sendo quantificada por cromatografia líquida de alta eficiência, e as concentrações mínima e máxima de 0,039mg/ml e 0,150mg/ml, respectivamente, com destaque para o isolado URM3812. Houve diferença estatística na quantidade de gliotoxina entre isolados da mesma espécie e o substrato de origem. Os isolados de A. flavus e A. niger não produziram gliotoxina nas condições estabelecidas. O modelo experimental foi estabelecido com sucesso utilizando os isolados URM3812, produtor de gliotoxina de origem ambiental e URM6753, não produtor de origem clínica, no qual ambos os isolados foram capazes de reproduzir a doença, não havendo diferenças no dano tecidual. Advisors/Committee Members: OLIVEIRA, Neiva Tinti de (advisor), MAGALHÃES, Oliane Maria Correia (advisor), http://lattes.cnpq.br/2531397248047256 (advisor).

Subjects/Keywords: Aspergillus; Melaninas; Genética

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APA (6^th Edition):

MORAES, H. M. d. S. O. (2015). Quantificação de melanina e gliotoxina e diversidade genética de isolados clínicos e ambientais de Aspergillus . (Doctoral Dissertation). Universidade Federal de Pernambuco. Retrieved from https://repositorio.ufpe.br/handle/123456789/26598

Chicago Manual of Style (16^th Edition):

MORAES, Heloiza Maria da Silva Oliveira. “Quantificação de melanina e gliotoxina e diversidade genética de isolados clínicos e ambientais de Aspergillus .” 2015. Doctoral Dissertation, Universidade Federal de Pernambuco. Accessed March 03, 2021. https://repositorio.ufpe.br/handle/123456789/26598.

MLA Handbook (7^th Edition):

MORAES, Heloiza Maria da Silva Oliveira. “Quantificação de melanina e gliotoxina e diversidade genética de isolados clínicos e ambientais de Aspergillus .” 2015. Web. 03 Mar 2021.

Vancouver:

MORAES HMdSO. Quantificação de melanina e gliotoxina e diversidade genética de isolados clínicos e ambientais de Aspergillus . [Internet] [Doctoral dissertation]. Universidade Federal de Pernambuco; 2015. [cited 2021 Mar 03]. Available from: https://repositorio.ufpe.br/handle/123456789/26598.

Council of Science Editors:

MORAES HMdSO. Quantificação de melanina e gliotoxina e diversidade genética de isolados clínicos e ambientais de Aspergillus . [Doctoral Dissertation]. Universidade Federal de Pernambuco; 2015. Available from: https://repositorio.ufpe.br/handle/123456789/26598

University of Debrecen

19. Kormány, Gábor. A növekedési szignálútvonal Aspergillus fonalas gombákban .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/108348

► A fonalas gombák életében a növekedési szignál transzdukciós kaszkád azon felül hogy, a növekedést biztosítja számos jelentős életfolyamatnak a szabályozásában részt vesz. ezeket a szabályozási… (more)

Subjects/Keywords: aspergillus; gomba; sporuláció

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APA (6^th Edition):

Kormány, G. (2011). A növekedési szignálútvonal Aspergillus fonalas gombákban . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/108348

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kormány, Gábor. “A növekedési szignálútvonal Aspergillus fonalas gombákban .” 2011. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/108348.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kormány, Gábor. “A növekedési szignálútvonal Aspergillus fonalas gombákban .” 2011. Web. 03 Mar 2021.

Vancouver:

Kormány G. A növekedési szignálútvonal Aspergillus fonalas gombákban . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/108348.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kormány G. A növekedési szignálútvonal Aspergillus fonalas gombákban . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/108348

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

20. Papp, Benedek. A lovasztatin termelése és piaci helyzete .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/118722

A lovasztatin fontos koleszterincsökkentő gyógyszer. Ipari előállítása fermentációval történik. A legfontosabb termelő törzs az Aspergillus terreus. Piaci helyzete számottevő. Advisors/Committee Members: Fekete, Erzsébet (advisor).

Subjects/Keywords: lovasztatin; Aspergillus terreus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Papp, B. (2011). A lovasztatin termelése és piaci helyzete . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/118722

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Papp, Benedek. “A lovasztatin termelése és piaci helyzete .” 2011. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/118722.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Papp, Benedek. “A lovasztatin termelése és piaci helyzete .” 2011. Web. 03 Mar 2021.

Vancouver:

Papp B. A lovasztatin termelése és piaci helyzete . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/118722.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Papp B. A lovasztatin termelése és piaci helyzete . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/118722

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

21. Nagy, Alexandra. Egy ismeretlen extracelluláris proteináz azonosítása Aspergillus nidulans szénéhező tenyészeteiben .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/118903

► Szakdolgozatom témája az Aspergillus niduland proteináz termelésének vizsgálata szénéhező körülmények között.Az autolízis egy aktív, energiaigényes, jól szabályozott természetes sejtpusztulási folyamat, mely során hidrolítikus enzimek (pl.… (more)

Subjects/Keywords: autolízis; Aspergillus; proteináz

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APA (6^th Edition):

Nagy, A. (2011). Egy ismeretlen extracelluláris proteináz azonosítása Aspergillus nidulans szénéhező tenyészeteiben . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/118903

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nagy, Alexandra. “Egy ismeretlen extracelluláris proteináz azonosítása Aspergillus nidulans szénéhező tenyészeteiben .” 2011. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/118903.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nagy, Alexandra. “Egy ismeretlen extracelluláris proteináz azonosítása Aspergillus nidulans szénéhező tenyészeteiben .” 2011. Web. 03 Mar 2021.

Vancouver:

Nagy A. Egy ismeretlen extracelluláris proteináz azonosítása Aspergillus nidulans szénéhező tenyészeteiben . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/118903.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nagy A. Egy ismeretlen extracelluláris proteináz azonosítása Aspergillus nidulans szénéhező tenyészeteiben . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/118903

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

22. Jenei, Beáta. Aspergillus nidulans törzsek keresztezésének vizsgálata .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2012, University of Debrecen

URL: http://hdl.handle.net/2437/152197

Aspergillus nidulans törzsek keresztezése és a kettős mutánsok előállításának menete. A. nidulans szaporodási formáinak bemutatása, kettős mutánsok előállítása. Advisors/Committee Members: Fekete, Erzsébet (advisor).

Subjects/Keywords: Aspergillus nidulans; keresztezés

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jenei, B. (2012). Aspergillus nidulans törzsek keresztezésének vizsgálata . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/152197

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jenei, Beáta. “Aspergillus nidulans törzsek keresztezésének vizsgálata .” 2012. Thesis, University of Debrecen. Accessed March 03, 2021. http://hdl.handle.net/2437/152197.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jenei, Beáta. “Aspergillus nidulans törzsek keresztezésének vizsgálata .” 2012. Web. 03 Mar 2021.

Vancouver:

Jenei B. Aspergillus nidulans törzsek keresztezésének vizsgálata . [Internet] [Thesis]. University of Debrecen; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2437/152197.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jenei B. Aspergillus nidulans törzsek keresztezésének vizsgálata . [Thesis]. University of Debrecen; 2012. Available from: http://hdl.handle.net/2437/152197

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of St. Andrews

23. Akhtar, Naureen. Nitrate transport and assimilation in Aspergillus nidulans .

Degree: 2012, University of St. Andrews

URL: http://hdl.handle.net/10023/3093

► In this study, several aspects of nitrate assimilation and transport have been studied using the filamentous fungus Aspergillus nidulans, which has been shown to be… (more)

▼ In this study, several aspects of nitrate assimilation and transport have been studied using the filamentous fungus Aspergillus nidulans, which has been shown to be safe laboratory organism as judged by it’s pathogenicity towards insect larvae. In silico analysis of the A. nidulans genome sequence, identified two putative genes designated cnxL and cnxK that might be involved in molybdenum cofactor (a component of nitrate reductase) biosynthesis as well as two putative nitrate reductases encoding genes niaB and niaC. All four genes are hitherto unknown. Although many features of these proteins provided clues of functionality, biochemical and genetical approaches employed in this present study failed to elicit expression of any of these four genes. A NrtA protein structure model was developed based on residue homology with the E. coli GlpT a protein, the structure of which has been solved. The results of thiol cross-linking of three double cysteine mutants in four NrtA essential residues, R87, R368, N168 and N459, indicated that the molecular distance between R87 and R368 is ~ 0.4 Å, R368 and N168 ~ 6.2 Å, R87 and N459 is ~ 2.2 Å. Another important observation was the change in the confirmation of Tm 2 and Tm 8 in the presence of nitrate. This shift resulted in an increase of ~ 2 Å gap between the residues R87 and R368. Distances between amino acid residue pairs estimated using such molecular rulers contradicted the NrtA existing model. Cysteine-scanning mutagenesis studies were extended to the generation of a library of single cysteine mutants of NrtA residues spanning Tm 2 and Tm 8. The majority of single cysteine mutants possessed wild type NrtA protein expression levels but unfortunately most were found to be loss-of-function. Consequently, thiol chemistry of this crop of mutants was not perused. Attempts were also made to overexpress and crystallise the bacterial nitrate transporters but none of the transporter tested proved to be a successful candidate for crystallisation. In this regard, bacterial nitrate transporters, NarU (E. coli), Nar (Bacillus cereus), NarK1 and NarK2 (Pseudomonas aeruginosa) and NarK2 (Thermus thermophilus) fused with GFP were expressed in E. coli and used in crystallisation trials. Although this approach has proved successful for a number of membrane proteins, unfortunately was not helpful with regard to the purification of any of the above bacterial nitrate transporters to yield protein expression levels required for successful protein crystallography. Finally, the effects of potential nitrate transport inhibitors were studied on net nitrate transport by NrtA and NrtB proteins of A. nidulans. The results indicated that chlorate had more of an inhibitory effect on NrtA net nitrate transport than that by NrtB. Chlorite and sulphite equally affected net nitrate transport by either NrtA or NrtB proteins while caesium strongly inhibited the net nitrate transport by NrtB transporter. Advisors/Committee Members: Kinghorn, James R (advisor), Unkles, Shiela (advisor).

Subjects/Keywords: Nitrate transporters; Aspergillus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Akhtar, N. (2012). Nitrate transport and assimilation in Aspergillus nidulans . (Thesis). University of St. Andrews. Retrieved from http://hdl.handle.net/10023/3093

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Akhtar, Naureen. “Nitrate transport and assimilation in Aspergillus nidulans .” 2012. Thesis, University of St. Andrews. Accessed March 03, 2021. http://hdl.handle.net/10023/3093.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Akhtar, Naureen. “Nitrate transport and assimilation in Aspergillus nidulans .” 2012. Web. 03 Mar 2021.

Vancouver:

Akhtar N. Nitrate transport and assimilation in Aspergillus nidulans . [Internet] [Thesis]. University of St. Andrews; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10023/3093.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Akhtar N. Nitrate transport and assimilation in Aspergillus nidulans . [Thesis]. University of St. Andrews; 2012. Available from: http://hdl.handle.net/10023/3093

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

24. Guazzelli, Luciana Silva. Estudo etiológico, clínico, laboratorial e epidemiológico da bola fúngica pulmonar por Aspergillus spp.

Degree: 2011, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/30929

► Descrição: Bola fúngica é definida como uma macrocolônia composta por emaranhado de hifas, células inflamatórias, fibrina, muco e fragmentos de tecidos. Aspergillus fumigatus é o… (more)

▼ Descrição: Bola fúngica é definida como uma macrocolônia composta por emaranhado de hifas, células inflamatórias, fibrina, muco e fragmentos de tecidos. Aspergillus fumigatus é o agente etiológico mais frequente, responsável por cerca de 90% dos casos, seguido de A niger e A flavus, respectivamente. O antecedente mais comum para o desenvolvimento da bola fúngica é cavidade secundária à tuberculose e a manifestação clínica mais presente e causadora de óbitos nesses pacientes é a hemoptise. Objetivos: Investigar as espécies de Aspergillus causadoras de bola fúngica pulmonar, determinar as condições predisponentes e/ou associadas e a comprovação laboratorial para o diagnóstico etiológico e observar a resposta as diferentes medidas terapêuticas dos pacientes com bola fúngica pulmonar. Delineamento: Foram analisados retrospectivamente, prontuários de pacientes para a caracterização da bola fúngica pulmonar por Aspergillus. Local do estudo: Laboratório de Micologia da Santa Casa Complexo Hospitalar, no período de 1980 a 2009. Pacientes e métodos: Foram incluídos neste estudo todos os pacientes com diagnóstico de bola fúngica pulmonar aspergilar de uma população de 750 casos de aspergilose, de 1980 a 2009. Os critérios para o diagnóstico foram os seguintes: isolamento da espécie de Aspergillus proveniente do material de cavidade pulmonar associado à imagem radiográfica compatível; isolamento da espécie de Aspergillus em outros materiais do trato respiratório, excluindo material da cavidade, associado ou não ao exame direto positivo; imunodifusão radial dupla positiva para Aspergillus associada ao exame de imagem compatível. Resultados: Foram incluídos 391 pacientes com bola fúngica pulmonar aspergilar, a idade variou de 18-78 anos, sendo 67,3% do gênero masculino. O diagnóstico foi baseado nos achados clínicos, radiológicos e laboratoriais. Em todos os pacientes foram detectados achados característicos de bola fúngica tanto no radiograma quanto na tomografia de tórax e bola fúngica complexa foi detectado em 97,4% da casuística. Tuberculose curada foi a principal condição predisponente (89%). Hemoptise foi manifestação clínica mais frequente (89%). A espécie A. fumigatus foi o agente etiológico mais isolado, 89,3% dos casos, seguido de A niger 7,1% e menos frequente A flavus 3,3%. A positividade no cultivo foi de 84,7% nos espécimes clínicos e a imunodifusão radial dupla de 81,6% dos pacientes. A principal medida terapêutica foi ressecção cirúrgica apresentando desfecho favorável em 88,3%. A eliminação da bola fúngica por lise espontânea ocorreu em 2,3% dos casos. Mortalidade foi atribuída à cirurgia e a hemoptise em 32,3 e 13,8%, respectivamente. Conclusões: Tuberculose curada e hemoptise é a primeira hipótese diagnóstica de bola fúngica pulmonar. O sinal radiológico indicativo de bola fúngica é cavidade contendo produto patológico com densidade de partes moles, espessamento da parede da cavidade e da pleura circunjacente. A detecção de anticorpos séricos por imunodifusão radial dupla, e o cultivo de espécimes do trato… Advisors/Committee Members: Severo, Luiz Carlos.

Subjects/Keywords: Fungus ball; Tuberculose; Aspergilose; Tuberculosis; Aspergillosis; Aspergillus fumigatus; Hemoptise; Aspergillus fumigatus; Aspergillus; Aspergillus niger; Aspergillus flavus; Hemoptysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guazzelli, L. S. (2011). Estudo etiológico, clínico, laboratorial e epidemiológico da bola fúngica pulmonar por Aspergillus spp. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/30929

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guazzelli, Luciana Silva. “Estudo etiológico, clínico, laboratorial e epidemiológico da bola fúngica pulmonar por Aspergillus spp.” 2011. Thesis, Universidade do Rio Grande do Sul. Accessed March 03, 2021. http://hdl.handle.net/10183/30929.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guazzelli, Luciana Silva. “Estudo etiológico, clínico, laboratorial e epidemiológico da bola fúngica pulmonar por Aspergillus spp.” 2011. Web. 03 Mar 2021.

Vancouver:

Guazzelli LS. Estudo etiológico, clínico, laboratorial e epidemiológico da bola fúngica pulmonar por Aspergillus spp. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10183/30929.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guazzelli LS. Estudo etiológico, clínico, laboratorial e epidemiológico da bola fúngica pulmonar por Aspergillus spp. [Thesis]. Universidade do Rio Grande do Sul; 2011. Available from: http://hdl.handle.net/10183/30929

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

25. Suleiman, M.M. (Mohammed Musa). The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts .

Degree: 2010, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-10062010-203419/

► The main aim of this study was to find a plant extract or isolated compound that could be used to combat aspergillosis in animals. Aspergillus… (more)

▼ The main aim of this study was to find a plant extract or isolated compound that could be used to combat aspergillosis in animals. Aspergillus fumigatus is one of the most common pathogenic fungal species in humans and animals. A. fumigatus is also an economically important fungus in the poultry industry. Current treatment of the disease is hampered by drug resistance of the organism to conventional antifungals and also its widespread toxicity to the animals. Seven tree species that had good antifungal activity against Cryptococcus neoformans in the Phytomedicine Programme database were selected for further work. These tree species were: Combretum vendae A.E. van Wyk (Combretaceae), Commiphora harveyi (Engl.) Engl. (Burseraceae), Khaya anthotheca (Welm.) C.DC (Meliaceae), Kirkia wilmsii Engl. (Kirkiaceae), Loxostylis alata A. Spreng. ex Rchb. (Anacardiaceae), Ochna natalitia (Meisn.) Walp. (Ochnaceae) and Protorhus longifolia (Bernh. Ex C. Krauss) Engl. (Anacardiaceae). The antimicrobial activity of leaf extracts of the selected plant species were determined against four important nosocomial bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa) and five important animal fungi (Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Microsporum canis and Sporothrix schenckii) using a serial microplate dilution method. The minimal inhibitory concentrations (MIC), of an acetone extract of Loxostylis alata was the lowest against Aspergillus fumigatus with an MIC value of 0.05 mg/ml. The number of antifungal compounds in extracts was determined by bioautography. The acetone extract of L. alata had the most active zones (10). The antioxidant, antiplatelet and cytotoxic effects of the seven plant species were evaluated using established in vitro assays. All the extracts had comparably low toxicity except for the extract of C. harveyi that had high haemagluttination assay titre value, which indicates toxicity. The extracts of P. longifolia, K. wilmsii, O. natalitia, L. alata, C. harveyi and C. vendae contained antioxidant compounds in the qualitative assay using DPPH. In the quantification of antioxidation using ABTS, only the extracts of P. longifolia, L. alata, and C. vendae had substantial antioxidant activity with respective TEAC value of 1.39, 1.94 and 2.08. Similarly, in the quantitative DPPH assay, L. alata. (EC50, 3.58 ± 0.23 μg/ml) and K. wilmsii (EC50, 3.57 ± 0.41 μg/ml) did not differ significantly (p ≤ 0.05) from the positive control (L-ascorbic acid). K. anthotheca had a much lower antioxidant activity (EC<su>50 176.40 ± 26.56 μg/ml), and differed significantly (p ≤ 0.05) from all the other extracts and control. In addition, the extract of C. vendae and C. harveyi had significant (p ≤ 0.05) antiplatelet activity and did not differ from the control (aspirin) with EC50 of 0.06 ± 0.01 μg/ml, 0.19 ± 0.00 μg/ml, respectively. Lower EC50 values in the antioxidant and antiplatelet studies are indicative of superior activity of the plant extract against oxidation… Advisors/Committee Members: Naidoo, Vinny (advisor), Eloff, Jacobus Nicolaas (advisor).

Subjects/Keywords: Aspergillus fumigatus; Fungal species; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suleiman, M. M. (. M. (2010). The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-10062010-203419/

Chicago Manual of Style (16^th Edition):

Suleiman, M M (Mohammed Musa). “The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts .” 2010. Doctoral Dissertation, University of Pretoria. Accessed March 03, 2021. http://upetd.up.ac.za/thesis/available/etd-10062010-203419/.

MLA Handbook (7^th Edition):

Suleiman, M M (Mohammed Musa). “The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts .” 2010. Web. 03 Mar 2021.

Vancouver:

Suleiman MM(M. The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts . [Internet] [Doctoral dissertation]. University of Pretoria; 2010. [cited 2021 Mar 03]. Available from: http://upetd.up.ac.za/thesis/available/etd-10062010-203419/.

Council of Science Editors:

Suleiman MM(M. The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata A.Spreng. ex Rchb. leaf extracts . [Doctoral Dissertation]. University of Pretoria; 2010. Available from: http://upetd.up.ac.za/thesis/available/etd-10062010-203419/

Universidade do Rio Grande do Sul

26. Boff, Cristiane. Monitoramento de fungos no ar de unidades de terapia intensiva.

Degree: 2011, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/53159

►

Nos ambientes hospitalares, crescente importância têm sido dada ao estudo dos bioaerossóis, principalmente em áreas onde pacientes suscetíveis a doenças causadas pela exposição a estes… (more)

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Nos ambientes hospitalares, crescente importância têm sido dada ao estudo dos bioaerossóis, principalmente em áreas onde pacientes suscetíveis a doenças causadas pela exposição a estes elementos estejam internados, como unidades hematológicas e de terapia intensiva. Entre os diferentes métodos utilizados para amostrar o ar no ambiente hospitalar, destaca-se o uso de amostradores que permitem a impactação direta de partículas em placas de ágar, a exemplo do coletor de Andersen®. Estes equipamentos fornecem informações qualitativas e quantitativas quanto à presença de conídios fúngicos nos ambientes avaliados, sendo possível relatar o resultado em unidades formadoras de colônia por metro cúbico de ar. Apesar de diversos estudos terem sido realizados para avaliar a qualidade do ar em ambientes internos, ainda não foi estabelecida uma padronização para níveis aceitáveis de fungos em ambientes não equipados com filtros de alta eficiência (HEPA). Além disso, a concentração de fungos nos ambientes sofre influências de diversas variáveis, incluindo as características físicas das partículas, fatores ambientais e outras situações, como a presença de obras e tipos de filtro de ar condicionado. Neste estudo, avaliou-se a qualidade do ar de duas unidades de terapia intensiva em hospitais universitários de Porto Alegre, no intuito de estudar a quantidade de fungos presentes nestas unidades, correlacionando os achados com variáveis ambientais e com o isolamento de fungos a partir de amostras clínicas.

Increasing importance has been given to the study of bioaerosols in hospital environments, mainly in areas where patients that are susceptible to diseases caused by fungal exposure are admitted, such as in hematological units and in the intensive care. Among the different methods used to sample the air in a hospital environment, emphasis has been put on the use of samplers that allow direct impaction of particles in agar plates such as the Andersen sampler. This equipment provides qualitative and quantitative information regarding the presence of fungal conidia in the environment, providing results in terms of number of colony forming units per m3 of sampled air. However, guidelines on how to interpret fungal concentrations in areas that are not equipped with high efficiency particulate air filters (HEPA) are not available. It is well known the concentration of fungi in the environments is influenced by several variables, including the physical characteristics of the particles, environmental factors and others, such as the presence of construction works and air conditioning filter types. In this study, we evaluated the air quality of three intensive care units in university hospitals in Porto Alegre. Our aim was to study the quantity of fungi present in these units, correlating the findings with environmental variables and the recovery of fungi from clinical samples.

Advisors/Committee Members: Pasqualotto, Alessandro Comaru.

Subjects/Keywords: Contagem de colônia microbiana; Aspergillus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boff, C. (2011). Monitoramento de fungos no ar de unidades de terapia intensiva. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/53159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boff, Cristiane. “Monitoramento de fungos no ar de unidades de terapia intensiva.” 2011. Thesis, Universidade do Rio Grande do Sul. Accessed March 03, 2021. http://hdl.handle.net/10183/53159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boff, Cristiane. “Monitoramento de fungos no ar de unidades de terapia intensiva.” 2011. Web. 03 Mar 2021.

Vancouver:

Boff C. Monitoramento de fungos no ar de unidades de terapia intensiva. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10183/53159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boff C. Monitoramento de fungos no ar de unidades de terapia intensiva. [Thesis]. Universidade do Rio Grande do Sul; 2011. Available from: http://hdl.handle.net/10183/53159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Lira, Diana Duarte de. Caracterização de Aspergillus sp. Quanto a capacidade de degradação de óleo diesel .

Degree: 2014, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/11900

► A taxonomia clássica de fungos filamentos é baseada em observações de características fenotípicas, além de incorporar as aplicações biotecnológicas das substâncias produzidas à otimização de… (more)

▼ A taxonomia clássica de fungos filamentos é baseada em observações de características fenotípicas, além de incorporar as aplicações biotecnológicas das substâncias produzidas à otimização de processos. Os fungos por participarem da ciclagem de material orgânico são hábeis em descontaminar ambientes. Este trabalho teve por objetivo a identificação de 23 espécies de Aspergillus isolados de água do mar e estuário com a capacidade de utilizar óleo diesel como fonte de carbono. Para taxonomia clássica foram utilizados meios diferenciais. Após a identificação foi realizado o teste com o indicador 2,6 diclofenolindolfenol, e foram selecionados os que apresentaram mudança da coloração do indicador com 24horas após inicio do ensaio. As linhagens selecionadas foram submetidas aos ensaios de aclimatação, otimização das condições nutricionais do meio de cultivo e toxicidade dos subprodutos provenientes do biotratamento utilizando o índice de germinação o qual analisa a geminação e crescimento médio de sementes vegetais. Para otimização de ensaio de degradação foi utilizado o planejamento experimental Delineamento Composto Central com três variáveis independentes: nitrogênio, fósforo e magnésio. Foram identificadas as espécies Aspergillus flavus(06), Aspergillus aculeatus(08), Aspergillus terreus(01), Aspergillus awamori(02), Aspergillus tamarii(01), Aspergillus japonicus(03), Aspergillus niger(02). Oito linhagens apresentaram viragem da coloração azul para incolor em 24 horas, mas o Aspergillus flavus (AFL59) foi selecionado para todos os testes por apresentar 80% de degradação. A linhagem submetida ao ensaio de otimização apresentou o Índice de Germinação com valores próximos a 100% crescimento da raiz e eficiência de degradação (E.D%) do óleo diesel próximo a 100% para o hidrocarboneto nonano, ausência de picos entre decano e hexadecano, e a partir de heptadecano até heneicosano as porcentagens variaram entre 20,85% e 37,11%. Concluímos que o tratamento de óleo diesel utilizando as linhagens de Aspergillus mostrou-se eficiente para degradação de cadeias de hidrocarbonetos maiores apresentando baixa toxicidade para o nível trófico utilizado para o ensaio. Advisors/Committee Members: Gusmão, Norma Buarque de (advisor), Motta, Cristina M. de Souza (advisor).

Subjects/Keywords: Biorremediação; Taxonomia; Aspergillus; Óleo diesel

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lira, D. D. d. (2014). Caracterização de Aspergillus sp. Quanto a capacidade de degradação de óleo diesel . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/11900

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lira, Diana Duarte de. “Caracterização de Aspergillus sp. Quanto a capacidade de degradação de óleo diesel .” 2014. Thesis, Universidade Federal de Pernambuco. Accessed March 03, 2021. http://repositorio.ufpe.br/handle/123456789/11900.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lira, Diana Duarte de. “Caracterização de Aspergillus sp. Quanto a capacidade de degradação de óleo diesel .” 2014. Web. 03 Mar 2021.

Vancouver:

Lira DDd. Caracterização de Aspergillus sp. Quanto a capacidade de degradação de óleo diesel . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2014. [cited 2021 Mar 03]. Available from: http://repositorio.ufpe.br/handle/123456789/11900.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lira DDd. Caracterização de Aspergillus sp. Quanto a capacidade de degradação de óleo diesel . [Thesis]. Universidade Federal de Pernambuco; 2014. Available from: http://repositorio.ufpe.br/handle/123456789/11900

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Lima, Juliana Silva de. Produção de tanases por espécies de Aspergillus e Penicillium para clarificação de suco de uva (Vitis vinífera L.) .

Degree: 2014, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/11902

► Espécies de Aspergillus e Penicillium são de extrema importância na natureza, pois participam de forma ativa nos ciclos biogeoquímicos, atuando na decomposição de matéria orgânica.… (more)

▼ Espécies de Aspergillus e Penicillium são de extrema importância na natureza, pois participam de forma ativa nos ciclos biogeoquímicos, atuando na decomposição de matéria orgânica. Devido à sua elevada competência metabólica, não são muito exigentes nutricionalmente e são tolerantes a uma imensa variedade de condições físico-químicas. A tanase é uma enzima extracelular, induzível produzida por fungos filamentosos, bactérias e leveduras através da Fermentação em Estado Solido (FES) ou Fermentação Submersa (FS). Os taninos são compostos fenólicos provenientes do metabolismo secundário de vegetais podendo ser encontrados em todas as partes da planta. Desta forma, alguns resíduos vegetais ricos em taninos podem ser utilizados como substrato para produção da tanase. O presente trabalho teve como objetivos testar linhagens de Aspergillus e Penicillium mantidas na micoteca URM quanto a produção de tanases através de FES, utilizando folhas e resíduos de acerola e de mangaba como substratos, otimizar a produção pela linhagem melhor produtora, caracterizar o extrato bruto e aplicação na clarificação do suco de uva (Vitis vinifera L). A determinação das melhores condições de produção da enzima foi realizada utilizando como ferramenta o Planejamento Placket-Burman (PB) e Metodologia de Superfície de Resposta (MSR). A enzima foi caracterizada quanto ao efeito e estabilidade ao pH e à temperatura. Todas as culturas testadas produziram tanase com atividade entre 3,57 e 32,52 U/mL, sendo P. montanense URM 6486 o melhor produtor utilizando resíduo de acerola como substrato. Na MSR foram encontrados como os melhores parâmetros para a produção de tanase: meio de cultura umidecido 70%, 3,5% de ácido tânico, 34°C, com atividade máxima de 41,64 U/mL. Penicillium montanense URM 6486 apresenta pH e temperatura ótimos a 9,0 e 50°C, respectivamente e a enzima foi estável toda faixa de pH e temperatura. As tanases produzida por P. montanense URM 6486 quando aplicadas ao suco de uva, apresentou maior eficiência ao reduzir 46% do teor de taninos presentes no suco, após 120 minutos à 37ºC. O resíduo de acerola demonstrou um grande potencial como substrato para a produção da enzima em estudo, minimizando os custos de produção e agregando valor ao resíduo, uma vez que a enzima tem alto custo. Advisors/Committee Members: Motta, Cristina Maria de Souza (advisor), Moreira, Keila Aparecida (advisor).

Subjects/Keywords: Tanase; Residuos agroindustriais; Aspergillus; Penicillium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lima, J. S. d. (2014). Produção de tanases por espécies de Aspergillus e Penicillium para clarificação de suco de uva (Vitis vinífera L.) . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/11902

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lima, Juliana Silva de. “Produção de tanases por espécies de Aspergillus e Penicillium para clarificação de suco de uva (Vitis vinífera L.) .” 2014. Thesis, Universidade Federal de Pernambuco. Accessed March 03, 2021. http://repositorio.ufpe.br/handle/123456789/11902.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lima, Juliana Silva de. “Produção de tanases por espécies de Aspergillus e Penicillium para clarificação de suco de uva (Vitis vinífera L.) .” 2014. Web. 03 Mar 2021.

Vancouver:

Lima JSd. Produção de tanases por espécies de Aspergillus e Penicillium para clarificação de suco de uva (Vitis vinífera L.) . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2014. [cited 2021 Mar 03]. Available from: http://repositorio.ufpe.br/handle/123456789/11902.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lima JSd. Produção de tanases por espécies de Aspergillus e Penicillium para clarificação de suco de uva (Vitis vinífera L.) . [Thesis]. Universidade Federal de Pernambuco; 2014. Available from: http://repositorio.ufpe.br/handle/123456789/11902

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Ait Mimoune, Nouara. Etude des champignons aflatoxinogenes et ochratoxinogenes isoles des fruits secs et des graines de sesame : influence de la temperature et l'activite de l'eau sur la production de l'aflatoxine B1.

Degree: 2012, Université M'Hamed Bougara Boumerdès

URL: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/126

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84 p. , ill. , 30 cm

Dans notre travail on s'est intéressé à l'étude de la contamination fongique de 57 échantillons de fruits secs… (more)

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84 p. , ill. , 30 cm

Dans notre travail on s'est intéressé à l'étude de la contamination fongique de 57 échantillons de fruits secs et graines oléagineuses (amandes, pistaches et graines de sésame) collectés dans différentes régions d'Algérie. Le dénombrement et l'identification de la flore fongique totale a démontré la dominance du genre Aspergillus (84,69%) suivi du genre Penicillium (9,31%). A partir de ces échantillons, 233 souches d'Aspergillus et 15 souches de Penicillium ont été isolées et testées pour leur capacité à produire les aflatoxines et l'ochratoxine A (OTA). Le potentiel toxinogène de ces différentes souches fongiques a été caractérisé sur milieu CAM pour les Aspergillus section Flavi et sur milieu CYA pour les autres isolats. La détection de la production des mycotoxines a été réalisée par CCM. Les résultats obtenus montrent que sur les 33 isolats d'A. carbonarius 93,93% sont ochratoxinogènes et aucun des isolats d'Aspergillus niger (105 isolats), d'Aspergillus ochraceus (4 isolats) et de Penicilliuim sp (15 isolats) ne produit l'OTA. Pour les 91 isolats d'Aspergillus appartenant à la section Flavi, 88 ont été identifiés comme étant des A. flavus et 3 sont rattachés à A. parasiticus. Ces dernières sont hautement aflatoxinogènes et produisent l'aflatoxine B et G. Pour les A. flavus 34,09% se sont révélés aflatoxinogènes et produisent l'AFB1 uniquement. La production de CPA et de sclérotes a été réalisée sur milieu CYA. Toutes les souches aflatoxinogènes d'A. flavus produisent le CPA alors que 29,2% ont produit des sclérotes de type " L ". Une étude portant sur l'influence de la température et de l'activité de l'eau (aw) sur la croissance et la production d'AFB1 d'une souche d'A. flavus aflatoxinogène a été également réalisée. Nous avons remarqué que l'humidité affecte sensiblement la production d'AFB1 par A. flavus. Une production optimale d'AFB1 a été détectée à 30°C et à une aw de 0,99

Subjects/Keywords: Aspergillus; Aflatoxines; Ochratoxines; Fruits secs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ait Mimoune, N. (2012). Etude des champignons aflatoxinogenes et ochratoxinogenes isoles des fruits secs et des graines de sesame : influence de la temperature et l'activite de l'eau sur la production de l'aflatoxine B1. (Thesis). Université M'Hamed Bougara Boumerdès. Retrieved from http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/126

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ait Mimoune, Nouara. “Etude des champignons aflatoxinogenes et ochratoxinogenes isoles des fruits secs et des graines de sesame : influence de la temperature et l'activite de l'eau sur la production de l'aflatoxine B1.” 2012. Thesis, Université M'Hamed Bougara Boumerdès. Accessed March 03, 2021. http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/126.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ait Mimoune, Nouara. “Etude des champignons aflatoxinogenes et ochratoxinogenes isoles des fruits secs et des graines de sesame : influence de la temperature et l'activite de l'eau sur la production de l'aflatoxine B1.” 2012. Web. 03 Mar 2021.

Vancouver:

Ait Mimoune N. Etude des champignons aflatoxinogenes et ochratoxinogenes isoles des fruits secs et des graines de sesame : influence de la temperature et l'activite de l'eau sur la production de l'aflatoxine B1. [Internet] [Thesis]. Université M'Hamed Bougara Boumerdès; 2012. [cited 2021 Mar 03]. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/126.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ait Mimoune N. Etude des champignons aflatoxinogenes et ochratoxinogenes isoles des fruits secs et des graines de sesame : influence de la temperature et l'activite de l'eau sur la production de l'aflatoxine B1. [Thesis]. Université M'Hamed Bougara Boumerdès; 2012. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/126

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Bensmail, Souhila. Optimisation de la production de la protéase acide par aspergillus niger sur milieu solide : purification et caractérisation.

Degree: 2012, Université M'Hamed Bougara Boumerdès

URL: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/133

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106 p. , ill. , 30 cm

La pénurie mondiale en présure à susciter le développement de la recherche des nouveaux succédanés capables de coaguler… (more)

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106 p. , ill. , 30 cm

La pénurie mondiale en présure à susciter le développement de la recherche des nouveaux succédanés capables de coaguler le lait, tout en assurant de bons rendements fromagers. Dans cet objectif, la production de la protéase acide extracellulaire par l’espèce fongique Aspergillus niger isolée localement, sur un résidu agroalimentaire peu couteux fermenté par des cultures solides (SSF), a été optimisée de façon à obtenir la meilleure activité coagulante. Un essai de purification et une caractérisation biochimique de l’enzyme ont été également effectués. Les résultats obtenus montrent que la meilleure production de cette enzyme (830 US/g avec un rapport AC/AP de 4,25) a été obtenue sur milieu à base de son de blé (10g), humidifié à un taux de 39,21% par une solution minérale Czapek-Dox à pH 4, incubé à 30°C pendant 72 heures et inoculé par la suspension fongique de 106 spores/ml. L’application du plan factoriel fractionnaire à l’optimisation de la production de la protéase acide d’A. niger, a permis de déterminer l’influence significative du taux d’humidité du substrat et de la quantité en CaCl2 dans le milieu (deux effets contradictoires positif et négatif respectivement) et d’exclure l’importance des autres facteurs du plan d’expérience (la quantité du lait écrémé et celle de la caséine). Une activité coagulante de 582 US/g et un rapport AC/AP de 4,32 ont été obtenus pendant cette étude. La protéase extracellulaire a été purifiée 8 fois avec un rendement d’environ 10%, en utilisant la précipitation au (NH4)2SO4 à 80% de saturation et la chromatographie échangeuse d'ions sur DEAE-A50. La purification était plus efficace dans ces conditions par rapport à l'utilisation de la chromatographie d'exclusion moléculaire sur gel de Sephadex-G100 comme l’étape qui suive la précipitation. L'enzyme a été trouvée sous une forme monomérique, ayant un poids moléculaire de 47,7kDa par SDS-PAGE, résultats confirmés par la zymographie. Les conditions optimales de l’activité coagulante de l’extrait enzymatique partiellement purifié ont été déterminées ; elles correspondent aux paramètres suivants: température de 45°C, pH 5 (protéase acide) et une concentration en CaCl2 de 0,04M. Il présente une stabilité dans la gamme du pH 3,0-5,0 à 4°C pendant 24 heures dans le tampon citrate de sodium (0,1M) et une stabilité thermique jusqu’à 45°C pendant 1 heure

Subjects/Keywords: Coagulation; Aspergillus niger; Lait; Purification

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bensmail, S. (2012). Optimisation de la production de la protéase acide par aspergillus niger sur milieu solide : purification et caractérisation. (Thesis). Université M'Hamed Bougara Boumerdès. Retrieved from http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/133

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bensmail, Souhila. “Optimisation de la production de la protéase acide par aspergillus niger sur milieu solide : purification et caractérisation.” 2012. Thesis, Université M'Hamed Bougara Boumerdès. Accessed March 03, 2021. http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/133.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bensmail, Souhila. “Optimisation de la production de la protéase acide par aspergillus niger sur milieu solide : purification et caractérisation.” 2012. Web. 03 Mar 2021.

Vancouver:

Bensmail S. Optimisation de la production de la protéase acide par aspergillus niger sur milieu solide : purification et caractérisation. [Internet] [Thesis]. Université M'Hamed Bougara Boumerdès; 2012. [cited 2021 Mar 03]. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/133.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bensmail S. Optimisation de la production de la protéase acide par aspergillus niger sur milieu solide : purification et caractérisation. [Thesis]. Université M'Hamed Bougara Boumerdès; 2012. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/133

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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