subject:(Aptamer)

Wake Forest University

1. Stuart, Christopher H. USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS.

Degree: 2016, Wake Forest University

► The development of safe and efficacious chemotherapeutics has been the goal of cancer researchers for decades. Many different methods have been used to pursue this… (more)

Subjects/Keywords: Aptamer

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APA (6^th Edition):

Stuart, C. H. (2016). USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/59294

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Stuart, Christopher H. “USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS.” 2016. Thesis, Wake Forest University. Accessed April 10, 2021. http://hdl.handle.net/10339/59294.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Stuart, Christopher H. “USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS.” 2016. Web. 10 Apr 2021.

Vancouver:

Stuart CH. USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS. [Internet] [Thesis]. Wake Forest University; 2016. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10339/59294.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Stuart CH. USE OF DNA APTAMERS FOR TARGETED DELIVERY OF CHEMOTHERAPEUTIC AGENTS. [Thesis]. Wake Forest University; 2016. Available from: http://hdl.handle.net/10339/59294

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

2. Hutanu, Daniela. Synthesis and characterization of novel stationary phases for small scale liquid chromatographic separations of proteins and nanoparticles.

Degree: PhD, Chemistry, 2008, Oregon State University

URL: http://hdl.handle.net/1957/8223

► The emerging field of nanotechnology strictly requires the micro-scaling of the available separation technology and the design of novel devices for separations of molecules of… (more)

▼ The emerging field of nanotechnology strictly requires the micro-scaling of the available separation technology and the design of novel devices for separations of molecules of interest. The separation of proteins and nanoparticles is challenging due to their relatively large size, non-specific adherence to surfaces and instability in many solvents. This dissertation presents the synthesis and characterization of novel stationary phases for use in separations of proteins or nanoparticles in both capillary and microchip formats. In order to separate blood proteins with high specificity, a DNA aptamer selected for α-thrombin was employed as an affinity component of the stationary phases. Silica surfaces and organic monoliths were modified with the aptamer via an azlactone linkage and have demonstrated highly efficient separations of thrombin from a mixture in the microscale. The high efficiency of the protein separation (HETP = 276 μm, RS = 1.7) is comparable with macroscale results using antibodies as the affinity factor. Novel hybrid inorganic-organic polysilsesquioxane stationary phases were synthesized by way of surfactant templated polymerization of bridged alcoxy-silyl ethane monomers, in presence of sodium hydroxide. The novel materials were successful in size exclusion separation of polystyrene standards with molecular diameters of 0.3-2.4 nm. A hybrid inorganic-organic polysilsesquioxane sorbent also proved useful for small scale separations of triphenyl phosphine protected gold nanoparticles, based on a sorptive mechanism instead of a size exclusion mechanism. Polysilsesquioxanes were easily synthesized in-situ inside fused silica capillary columns and PMMA microchip channels in order to facilitate integration with a micro-reactor. The novel stationary phases proved efficient for separation of proteins and nanoparticles in the micro-scale format and can further be utilized for online purification and separation of these difficult compounds. Advisors/Committee Members: REMCHO, VINCENT T. (advisor), INGLE, JAMES (committee member).

Subjects/Keywords: APTAMER; Nanotechnology

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APA (6^th Edition):

Hutanu, D. (2008). Synthesis and characterization of novel stationary phases for small scale liquid chromatographic separations of proteins and nanoparticles. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/8223

Chicago Manual of Style (16^th Edition):

Hutanu, Daniela. “Synthesis and characterization of novel stationary phases for small scale liquid chromatographic separations of proteins and nanoparticles.” 2008. Doctoral Dissertation, Oregon State University. Accessed April 10, 2021. http://hdl.handle.net/1957/8223.

MLA Handbook (7^th Edition):

Hutanu, Daniela. “Synthesis and characterization of novel stationary phases for small scale liquid chromatographic separations of proteins and nanoparticles.” 2008. Web. 10 Apr 2021.

Vancouver:

Hutanu D. Synthesis and characterization of novel stationary phases for small scale liquid chromatographic separations of proteins and nanoparticles. [Internet] [Doctoral dissertation]. Oregon State University; 2008. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/1957/8223.

Council of Science Editors:

Hutanu D. Synthesis and characterization of novel stationary phases for small scale liquid chromatographic separations of proteins and nanoparticles. [Doctoral Dissertation]. Oregon State University; 2008. Available from: http://hdl.handle.net/1957/8223

University of Ottawa

3. Al-Youssef, Nadia. The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity .

Degree: 2015, University of Ottawa

URL: http://hdl.handle.net/10393/33182

► CD20 is an important oncological B-cell marker. Immunotherapy, using anti-CD20 antibodies, has revolutionized the treatment of B-cell cancers. Aptamers are highly specific DNA ligands, raised… (more)

Subjects/Keywords: aptamer; CD20

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APA (6^th Edition):

Al-Youssef, N. (2015). The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/33182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Al-Youssef, Nadia. “The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity .” 2015. Thesis, University of Ottawa. Accessed April 10, 2021. http://hdl.handle.net/10393/33182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Al-Youssef, Nadia. “The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity .” 2015. Web. 10 Apr 2021.

Vancouver:

Al-Youssef N. The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity . [Internet] [Thesis]. University of Ottawa; 2015. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10393/33182.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Al-Youssef N. The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity . [Thesis]. University of Ottawa; 2015. Available from: http://hdl.handle.net/10393/33182

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Kassahun, Getnet sewnet. Aptacapteur impédimétrique à base d’hydrogel pour la détection du Diclofenac : Hydrogel matrix grafted impedimetric aptasensor for the detection of Diclofenac.

Degree: Docteur es, Chimie Moléculaire, 2019, Paris Sciences et Lettres (ComUE)

URL: http://www.theses.fr/2019PSLEC027

►

-Une grande variété de polluants émergents se trouvent dans les sources d’eau naturelles et traitées. Les méthodestraditionnelles de détection et de quantification de ces polluants… (more)

▼

-Une grande variété de polluants émergents se trouvent dans les sources d’eau naturelles et traitées. Les méthodestraditionnelles de détection et de quantification de ces polluants font appel à la spectrométrie de masse, souventassociées à la chromatographie en phase gazeuse ou liquide. Ils sont coûteux, lents, nécessitent de gros appareillageset mobilisent des experts. Pour surmonter ces limitations, la mise au point de capteurs rapides, économiques etfaciles d’utilisation, destinés à l’analyse des polluants dans l’eau, revêt une importance capitale. Ces dernièresannées les biocapteurs électrochimiques font l’objet d’une attention considérable pour la détection et laquantification des polluants dans l’eau. Ils offrent l’avantage de détecter les contaminants à l’état de traces dansdifférentes matrices, telles que les échantillons d’eaux naturelles et traitées. Nous avons développé un nouveaubiocapteur basé sur l’utilisation d’un aptamère pour la reconnaissance moléculaire d'un polluant émergentpharmaceutique : le diclofénac (DCL). Une nouvelle classe de polymères a été utilisée comme matricebiocompatible pour l’immobilisation de l’aptamère. Il s’agit d’un film mince d'hydrogel greffé à la surface dutransducteur conducteur. L’immobilisation de l’aptamère sur l’hydrogel offre un environnement biodégradable quipermet de préserver la structure active et fonctionnelle de l’aptamère tout en permettant la détection du DCL. Legreffage de l’aptamère s’obtient par la formation de liaisons amides via l’activation des groupes acide carboxyliquede l’hydrogel Poly(Acide Acrylique) (PAA). La sensibilité du biocapteur est améliorée grâce à la densité de greffageélevée de l’apatmère et à la structure 3D de l’hydrogel. La spectroscopie d'impédance électrochimique (EIS) a étéutilisée pour détecter le DCL dans l’eau. La variation de la résistance au transfert de charge est linéaire avec uneconcentration cible comprise entre 30 pM et 1 μM. La limite de détection est de 0,02 nM.

-Fabrication of Surface-Attached hydrogel immobilization matrix thin films with tunable and well controlled chemistry -Functionalization and immobilization of the biorecognition agent -Characterization and Performances of developed polymeric biosensing surfaces

Advisors/Committee Members: Bedioui, Fethi (thesis director), Slim, Cyrine (thesis director), Griveau, Sophie (thesis director).

Subjects/Keywords: Biosensor; Aptamer; Hydrogel; Aptamer; Biosensor; Hydrogel

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APA (6^th Edition):

Kassahun, G. s. (2019). Aptacapteur impédimétrique à base d’hydrogel pour la détection du Diclofenac : Hydrogel matrix grafted impedimetric aptasensor for the detection of Diclofenac. (Doctoral Dissertation). Paris Sciences et Lettres (ComUE). Retrieved from http://www.theses.fr/2019PSLEC027

Chicago Manual of Style (16^th Edition):

Kassahun, Getnet sewnet. “Aptacapteur impédimétrique à base d’hydrogel pour la détection du Diclofenac : Hydrogel matrix grafted impedimetric aptasensor for the detection of Diclofenac.” 2019. Doctoral Dissertation, Paris Sciences et Lettres (ComUE). Accessed April 10, 2021. http://www.theses.fr/2019PSLEC027.

MLA Handbook (7^th Edition):

Kassahun, Getnet sewnet. “Aptacapteur impédimétrique à base d’hydrogel pour la détection du Diclofenac : Hydrogel matrix grafted impedimetric aptasensor for the detection of Diclofenac.” 2019. Web. 10 Apr 2021.

Vancouver:

Kassahun Gs. Aptacapteur impédimétrique à base d’hydrogel pour la détection du Diclofenac : Hydrogel matrix grafted impedimetric aptasensor for the detection of Diclofenac. [Internet] [Doctoral dissertation]. Paris Sciences et Lettres (ComUE); 2019. [cited 2021 Apr 10]. Available from: http://www.theses.fr/2019PSLEC027.

Council of Science Editors:

Kassahun Gs. Aptacapteur impédimétrique à base d’hydrogel pour la détection du Diclofenac : Hydrogel matrix grafted impedimetric aptasensor for the detection of Diclofenac. [Doctoral Dissertation]. Paris Sciences et Lettres (ComUE); 2019. Available from: http://www.theses.fr/2019PSLEC027

Penn State University

5. Chen, Niancao. Programmable Nanomaterials for Detoxification.

Degree: 2014, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/23701

► Exogenous chemicals (e.g., drugs) and endogenous signaling molecules (e.g., growth factors) are important for the treatment of human diseases and the maintenance of a normal… (more)

▼ Exogenous chemicals (e.g., drugs) and endogenous signaling molecules (e.g., growth factors) are important for the treatment of human diseases and the maintenance of a normal metabolism in the body. However, they can cause severe or fatal toxicity problems when their concentrations in the body exceed certain ranges1–3. To mitigate their toxic effects on the body, a myriad of antidotes have been developed. Of them, nanoparticles (e.g., liposomes) have recently attracted the most attention because nanoparticles can act as a sink to sequester toxic molecules more efficiently 4,5. Despite their promise, most nanoparticles have relatively low affinity in sequestering target molecules and slow sequestration rates. Moreover, currently available nanoparticle antidotes cannot be actively regulated to control their capability of sequestering target molecules. As a result, when nanoparticles are applied to sequester endogenous signaling molecules, these molecules may be over eliminated, which can also cause severe effects or even fatality. Thus, it is important that nanoparticle antidotes provide molecularly controllable target sequestration, which has never been studied before. This dissertation research explores the concept of bidirectional molecular recognition control for the development of an open, programmable nanoscale antidote. This nanoscale antidote can not only sequester target molecules effectively and rapidly, but also release target molecules via molecular interactions on demand. To construct the antidote, DNA oligonucleotides were used as programmable building blocks and affinity ligands (i.e., nucleic acid aptamer) for the synthesis of both linear and branched affinity DNA polymers (DPs). A magnetic nanoparticle was used as a nanoscaffold to support the growth of the DPs on its surface. Each repeating unit of the DP has the capability of target sequestration. The sequestration functionality of this programmable nanoparticle antidote was evaluated by using both a small molecule drug and large molecule protein. This novel antidote was also examined by evaluating its capabilities in mitigating the biological effects of the drug and the protein. Moreover the reversing of target sequestration via molecular regulation was validated. This dissertation has four major chapters. In Chapter 1, current strategies for developing nanoparticle-based antidotes are systematically reviewed. In Chapter 2, the synthesis of affinity DNA polymer-functionalized nanoparticles is introduced. In Chapter 3, the functionalities of the programmable antidote for detoxification is demonstrated in vitro. In Chapter 4, the ability to program the function of the antidote via molecular regulation is validated. The data suggest that this antidote can sequester both small molecules and large proteins. Importantly, this antidote can be programmed via strand displacement to control the molecular sequestration. The success of this study has opened a new avenue for the development of antidotes for safe and effective detoxification of either… Advisors/Committee Members: Yong Wang, Dissertation Advisor/Co-Advisor, Yong Wang, Committee Chair/Co-Chair, Jian Yang, Committee Member, Christine Dolan Keating, Committee Member, Manish Kumar, Committee Member.

Subjects/Keywords: Aptamer; Nanoparticle; detoxification

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APA (6^th Edition):

Chen, N. (2014). Programmable Nanomaterials for Detoxification. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/23701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chen, Niancao. “Programmable Nanomaterials for Detoxification.” 2014. Thesis, Penn State University. Accessed April 10, 2021. https://submit-etda.libraries.psu.edu/catalog/23701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chen, Niancao. “Programmable Nanomaterials for Detoxification.” 2014. Web. 10 Apr 2021.

Vancouver:

Chen N. Programmable Nanomaterials for Detoxification. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Apr 10]. Available from: https://submit-etda.libraries.psu.edu/catalog/23701.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chen N. Programmable Nanomaterials for Detoxification. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/23701

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University of Wellington

6. Li, Shiwei. Selection and characterisation of single-stranded DNA aptamers for triclosan.

Degree: 2016, Victoria University of Wellington

URL: http://hdl.handle.net/10063/5017

► Triclosan (TCS) is a chlorinated organic compound which, due to its antibacterial properties in vitro, has found widespread usages in many medical and consumer products… (more)

▼ Triclosan (TCS) is a chlorinated organic compound which, due to its antibacterial properties in vitro, has found widespread usages in many medical and consumer products such as textiles, plastics and personal care products. Humans are directly and chronically exposed to TCS via dermal and mucosal contact from the use of TCS-formulated products such as soap and toothpaste. TCS is classified as an environmental contaminant by the European Union Water Framework Directive, whose mandatory goal is to develop new and simple-to-use analytical methodologies capable of measuring low concentrations of TCS and that are suitable for high-throughput detection. Synthetically-derived single-stranded oligonucleotides, also known as aptamers, are superior candidates for the development of sensitive and high-throughput biosensing strategies. Biosensors utilising aptamers as molecular recognition elements have showed great promise in a variety of diagnostic and therapeutic applications, especially for the detection of small molecular weight organic compounds such as TCS. The aim of this thesis was to develop aptamers as new capture reagents for TCS, as the first step towards the development of an alternative, user-friendly, diagnostic technique for monitoring TCS in both environmental and biological samples. The objectives of the thesis were to: [i] produce by in vitro selection procedures, TCS binding single-stranded DNA (ssDNA) aptamers; [ii] characterise the selected aptamers and determine their equilibrium dissociation constant (Kd) values and; [iii] evaluate the applicability of the selected aptamers in a biosensing platform. To achieve these objectives, ssDNA aptamers capable of binding TCS were generated in vitro using a sequential approach known as systematic evolution of ligands by exponential enrichment (SELEX). An affinity column-based SELEX strategy together with a variety of SELEX modifications such as negative and counter selections, real-time amplification and fluorescence quantification were explored for finding TCS specific aptamers. A total of 20 TCS aptamers, ten from 8 rounds of a basic-SELEX procedure, and the other ten from 10 rounds of a revised-SELEX procedure were generated. In general, these aptamers showed acceptable levels of sensitivity and specificity to TCS, and the best binding aptamer demonstrated a Kd value of 378 nM. The Kd value is comparable to published Kd values for compounds that share similar chemical structures to TCS. In addition, a novel fluorescent-based imaging method was developed in this dissertation. The method developed provides an alternative approach for monitoring SELEX progression and has the potential to simplify the way to characterise the binding properties of an aptamer to its cognate target. The utility of this method was compared with commonly used methods such as dot blot and fluorescent binding assays. The performance of the new imaging method was superior to the existing methods in terms of accuracy, simplicity and reproducibility. Furthermore, the best binding TCS… Advisors/Committee Members: McNatty, Kenneth.

Subjects/Keywords: Aptamer; Triclosan; Aptasensor

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APA (6^th Edition):

Li, S. (2016). Selection and characterisation of single-stranded DNA aptamers for triclosan. (Doctoral Dissertation). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/5017

Chicago Manual of Style (16^th Edition):

Li, Shiwei. “Selection and characterisation of single-stranded DNA aptamers for triclosan.” 2016. Doctoral Dissertation, Victoria University of Wellington. Accessed April 10, 2021. http://hdl.handle.net/10063/5017.

MLA Handbook (7^th Edition):

Li, Shiwei. “Selection and characterisation of single-stranded DNA aptamers for triclosan.” 2016. Web. 10 Apr 2021.

Vancouver:

Li S. Selection and characterisation of single-stranded DNA aptamers for triclosan. [Internet] [Doctoral dissertation]. Victoria University of Wellington; 2016. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10063/5017.

Council of Science Editors:

Li S. Selection and characterisation of single-stranded DNA aptamers for triclosan. [Doctoral Dissertation]. Victoria University of Wellington; 2016. Available from: http://hdl.handle.net/10063/5017

University of Illinois – Urbana-Champaign

7. Akki, Spurti Umesh. Selecting DNA aptamers for 17β-estradiol.

Degree: MS, 0231, 2013, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/42165

► 17β-estradiol (E2) is a potent estrogen that has been widely documented in water resources. It falls under the category of endocrine disrupting compounds (EDCs), and… (more)

Subjects/Keywords: DNA aptamer; estradiol

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APA (6^th Edition):

Akki, S. U. (2013). Selecting DNA aptamers for 17β-estradiol. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/42165

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Akki, Spurti Umesh. “Selecting DNA aptamers for 17β-estradiol.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed April 10, 2021. http://hdl.handle.net/2142/42165.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Akki, Spurti Umesh. “Selecting DNA aptamers for 17β-estradiol.” 2013. Web. 10 Apr 2021.

Vancouver:

Akki SU. Selecting DNA aptamers for 17β-estradiol. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/2142/42165.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Akki SU. Selecting DNA aptamers for 17β-estradiol. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/42165

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

8. Szeto, Kylan. Development And Characterization Of High-Throughput Methods And Technologies For In Vitro Rna Aptamer Selections.

Degree: PhD, Applied Physics, 2014, Cornell University

URL: http://hdl.handle.net/1813/37086

► Aptamers are an emerging class of molecules that are valued for their ability to bind with high affinity and specificity to a desired target. These… (more)

▼ Aptamers are an emerging class of molecules that are valued for their ability to bind with high affinity and specificity to a desired target. These DNA or RNA ligands can be synthesized easily in the lab making them much more stable, reproducible, and cost-effective than other affinity reagents such as antibodies. These molecules possess a diverse and versatile set of abilities upon binding, making them useful not only in biotechnology and diagnostics, but also in therapeutics. Aptamers are typically generated through an in vitro process called SELEX (Systematic Evolution of Ligands by EXponential Enrichment). SELEX is an iterative process whereby a library of random sequences is exposed to a target, and bound sequences are selected and amplified making a pool enriched for improved binding over the original library. This process can be repeated until the best binding sequence dominates the pool. In this way, aptamers can be generated to nearly any conceivable target or molecule, overcoming the immunological limitations in generating antibodies. By manipulating the conditions within this selection process, aptamers can also be generated to bind in non-physiological environments, giving them the ability to perform in diverse applications. To help accelerate the discovery of aptamers, we developed a modular microcolumn technology that decreases reagent consumption, while permitting multiplex SELEX, allowing for more sophisticated selection schemes. By characterizing aptamer enrichments for all the available parameters, we have significantly increased the selection efficiency, while illustrating the failures of simple binding theories to non-classical modes of selection. We have also demonstrated the power of high-throughput sequencing for early identification of aptamers before they fully converge. This was used to validate a new method which optimizes selection time over individual selection cycle efficiencies. Surprisingly, our results also show the new method significantly improves selection efficiency, bringing into question some common beliefs derived from SELEX theory. Finally, we scaled our microcolumn technology to an automatable 96-well microplate format, and demonstrated its utility by characterizing and validating specific, non-specific, and background binding behavior of several RNA aptamers. The results reveal binding behaviors that fundamentally limit the performance and sensitivity of aptamer selections. Advisors/Committee Members: Craighead, Harold G (chair), Lis, John T (committee member), Zipfel, Warren R. (committee member), Muller, David Anthony (committee member).

Subjects/Keywords: Aptamer; high-throughput; SELEX

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APA (6^th Edition):

Szeto, K. (2014). Development And Characterization Of High-Throughput Methods And Technologies For In Vitro Rna Aptamer Selections. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/37086

Chicago Manual of Style (16^th Edition):

Szeto, Kylan. “Development And Characterization Of High-Throughput Methods And Technologies For In Vitro Rna Aptamer Selections.” 2014. Doctoral Dissertation, Cornell University. Accessed April 10, 2021. http://hdl.handle.net/1813/37086.

MLA Handbook (7^th Edition):

Szeto, Kylan. “Development And Characterization Of High-Throughput Methods And Technologies For In Vitro Rna Aptamer Selections.” 2014. Web. 10 Apr 2021.

Vancouver:

Szeto K. Development And Characterization Of High-Throughput Methods And Technologies For In Vitro Rna Aptamer Selections. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/1813/37086.

Council of Science Editors:

Szeto K. Development And Characterization Of High-Throughput Methods And Technologies For In Vitro Rna Aptamer Selections. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/37086

McMaster University

9. Shang, Jieting. MONITORING YEAST tRNA ADSORPTION BY QCM-D: AN OPPORTUNITY FOR OPTIMIZATION OF APTAMER SELECTION CONDITIONS.

Degree: MASc, 2014, McMaster University

URL: http://hdl.handle.net/11375/16365

►

RNA aptamers that bind to a wide range of targets with high affinity and specificity have been identified via the in vitro systematic evolution of… (more)

▼

RNA aptamers that bind to a wide range of targets with high affinity and specificity have been identified via the in vitro systematic evolution of ligands by exponential enrichment (SELEX). However, the process is quite unpredictable due in part to binding that occurs not only on the targets themselves but also on any of the other functional groups, moieties, or surfaces. Recent modelling work has shown that this level of “background binding” is a key parameter in the performance of aptamer selection processes. One strategy to minimize the amount of background binding is to pre-block those possible binding sites with a non-amplifiable nucleic acid molecule, such as yeast tRNA. It is also known that binding buffer conditions have strong effect on the binding affinity of nucleic acids. However, there are no detailed studies and little quantitative information available to guide the design of aptamer selection processes. In this study, the binding ability of yeast tRNA, which has comparable size with most RNA aptamer libraries, on both silicon dioxide and poly (ethylene terephthalate glycol) (PET-G) surfaces was studied using Quartz Crystal Microbalance with Dissipation (QCM-D). Silicon dioxide surface is a commonly used substrate for QCM-D tests on the adsorption behaviour of different nucleic acid. PET-G is a commonly used polymer substrate for the fabrication of microfluidic devices, which are advanced techniques for aptamer selection. The presence of specific divalent cations, for example Mg2+ over Ca2+, in binding buffers greatly enhanced the binding of yeast tRNA on silicon dioxide surfaces and PET-G surfaces. Proper NaCl concentration (100 mM) and MgCl2 concentration (5 mM) is necessary to enhance yeast tRNA binding on both surfaces. Yeast tRNA binding ability on silicon dioxide surfaces show more dependence on binding buffer pH than on PET-G surfaces.

Thesis

Master of Applied Science (MASc)

Advisors/Committee Members: Latulippe, David, Chemical Engineering.

Subjects/Keywords: QCM-D; aptamer; RNA; SELEX

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APA (6^th Edition):

Shang, J. (2014). MONITORING YEAST tRNA ADSORPTION BY QCM-D: AN OPPORTUNITY FOR OPTIMIZATION OF APTAMER SELECTION CONDITIONS. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/16365

Chicago Manual of Style (16^th Edition):

Shang, Jieting. “MONITORING YEAST tRNA ADSORPTION BY QCM-D: AN OPPORTUNITY FOR OPTIMIZATION OF APTAMER SELECTION CONDITIONS.” 2014. Masters Thesis, McMaster University. Accessed April 10, 2021. http://hdl.handle.net/11375/16365.

MLA Handbook (7^th Edition):

Shang, Jieting. “MONITORING YEAST tRNA ADSORPTION BY QCM-D: AN OPPORTUNITY FOR OPTIMIZATION OF APTAMER SELECTION CONDITIONS.” 2014. Web. 10 Apr 2021.

Vancouver:

Shang J. MONITORING YEAST tRNA ADSORPTION BY QCM-D: AN OPPORTUNITY FOR OPTIMIZATION OF APTAMER SELECTION CONDITIONS. [Internet] [Masters thesis]. McMaster University; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/11375/16365.

Council of Science Editors:

Shang J. MONITORING YEAST tRNA ADSORPTION BY QCM-D: AN OPPORTUNITY FOR OPTIMIZATION OF APTAMER SELECTION CONDITIONS. [Masters Thesis]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16365

University of Ottawa

10. Bushnik, Evan. Partially Robotic Selection of Aptamers to Red Blood Cell Protein Glycophorin A .

Degree: 2018, University of Ottawa

URL: http://hdl.handle.net/10393/38175

► Aptamers are small DNA ligands that have been manually selected to strongly and specifically bind a target of interest. These molecules may prove superior to… (more)

Subjects/Keywords: Aptamer; Glycophorin; Blood; Emulsion

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APA (6^th Edition):

Bushnik, E. (2018). Partially Robotic Selection of Aptamers to Red Blood Cell Protein Glycophorin A . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/38175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bushnik, Evan. “Partially Robotic Selection of Aptamers to Red Blood Cell Protein Glycophorin A .” 2018. Thesis, University of Ottawa. Accessed April 10, 2021. http://hdl.handle.net/10393/38175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bushnik, Evan. “Partially Robotic Selection of Aptamers to Red Blood Cell Protein Glycophorin A .” 2018. Web. 10 Apr 2021.

Vancouver:

Bushnik E. Partially Robotic Selection of Aptamers to Red Blood Cell Protein Glycophorin A . [Internet] [Thesis]. University of Ottawa; 2018. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10393/38175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bushnik E. Partially Robotic Selection of Aptamers to Red Blood Cell Protein Glycophorin A . [Thesis]. University of Ottawa; 2018. Available from: http://hdl.handle.net/10393/38175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

11. Fadock, Kaila. Aryl-Deoxyguanosine Probes: Application in G-Quadruplex Forming Aptamers.

Degree: PhD, Department of Chemistry, 2016, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9869

► Aptamers are laboratory created single-strands of DNA or RNA that have been designed to bind to a specific target with high affinity and specificity, and… (more)

▼ Aptamers are laboratory created single-strands of DNA or RNA that have been designed to bind to a specific target with high affinity and specificity, and are gaining popularity over antibody detection due to their economical and ethical advantages. With recent developments in the field of aptamer technology, the desire for fluorescent probes that can be incorporated within an oligonucleotide strand, without disrupting it's activity, has increased. Addition of an aryl group at the C8 site of deoxyguanosine (dG) affords addition products (adducts) which can have a variety of fluorescence capabilities. These fluorescent nucleosides have been known to be sensitive to their solvent environment, base stacking, and H-bonding interactions, making them ideal candidates for development as fluorescent probes within aptamers. Aside from possessing fluorescence capabilities, the formation of a C8-aryl-dG adduct alters the conformational preference of the nucleoside. The probes are known to prefer the -syn¬-conformation, where rotation about its glycosidic bond minimizes steric interactions with the bulky aromatic group. This conformational preference can be exploited to influence the stability of a folded aptamer, and probe the structural features of the aptamer-target complex. A common folded motif within aptamers is the G-quadruplex (GQ). Insertion of probes within the core structure of the GQ has been fairly limited to date because current probes available are not able to be inserted in the place of a dG base, without disruption of the folded structure. The C8-aryl-dG probes presented in this thesis stabilize the folded GQ when placed within a syn-G, and have been optimized within a duplex to GQ exchange detection platform with thrombin binding aptamer (TBA). The utility of these probes were highlighted with insertion of 8-(2"-thienyl)-dG within the ochratoxin A aptamer to determine its structural properties, as well as probing the structural polymorphism of the human telomeric repeat sequence (HTelo). In order to create a series of probes that could be excited by visible light, further probe development was applied towards the creation of a series of push-pull adducts that contained molecular-rotor light switching abilities. Advisors/Committee Members: Manderville, Richard (advisor).

Subjects/Keywords: oligonucleotides; quadruplex; adduct; deoxyguanosine; aptamer

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APA (6^th Edition):

Fadock, K. (2016). Aryl-Deoxyguanosine Probes: Application in G-Quadruplex Forming Aptamers. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9869

Chicago Manual of Style (16^th Edition):

Fadock, Kaila. “Aryl-Deoxyguanosine Probes: Application in G-Quadruplex Forming Aptamers.” 2016. Doctoral Dissertation, University of Guelph. Accessed April 10, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9869.

MLA Handbook (7^th Edition):

Fadock, Kaila. “Aryl-Deoxyguanosine Probes: Application in G-Quadruplex Forming Aptamers.” 2016. Web. 10 Apr 2021.

Vancouver:

Fadock K. Aryl-Deoxyguanosine Probes: Application in G-Quadruplex Forming Aptamers. [Internet] [Doctoral dissertation]. University of Guelph; 2016. [cited 2021 Apr 10]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9869.

Council of Science Editors:

Fadock K. Aryl-Deoxyguanosine Probes: Application in G-Quadruplex Forming Aptamers. [Doctoral Dissertation]. University of Guelph; 2016. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9869

University of Guelph

12. Van Riesen, Abigail. Fluorescent Modified Nucleosides and Their Impact on Structure and Function of a Model Aptamer.

Degree: MS, Department of Chemistry, 2016, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10018

► A number of non-natural nucleic acid monomers were synthesized and employed in a model system in an effort to demonstrate the potential for expanding the… (more)

Subjects/Keywords: aptamer; G-quadruplex; thrombin; fluorescence

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APA (6^th Edition):

Van Riesen, A. (2016). Fluorescent Modified Nucleosides and Their Impact on Structure and Function of a Model Aptamer. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10018

Chicago Manual of Style (16^th Edition):

Van Riesen, Abigail. “Fluorescent Modified Nucleosides and Their Impact on Structure and Function of a Model Aptamer.” 2016. Masters Thesis, University of Guelph. Accessed April 10, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10018.

MLA Handbook (7^th Edition):

Van Riesen, Abigail. “Fluorescent Modified Nucleosides and Their Impact on Structure and Function of a Model Aptamer.” 2016. Web. 10 Apr 2021.

Vancouver:

Van Riesen A. Fluorescent Modified Nucleosides and Their Impact on Structure and Function of a Model Aptamer. [Internet] [Masters thesis]. University of Guelph; 2016. [cited 2021 Apr 10]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10018.

Council of Science Editors:

Van Riesen A. Fluorescent Modified Nucleosides and Their Impact on Structure and Function of a Model Aptamer. [Masters Thesis]. University of Guelph; 2016. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10018

13. Ray, Judhajeet. Aptamer sensors for live-cell imaging of Pol II promoter activity.

Degree: 2014, Iowa State University

URL: https://lib.dr.iastate.edu/etd/14283

► Development of multicellular organisms depends on spatial and temporal expression of certain genes. The spatio-temporal control is achieved by several factors involved in the key… (more)

Subjects/Keywords: Biochemistry; Aptamer; Imaging; Transcription; Biochemistry

11 more images…

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APA (6^th Edition):

Ray, J. (2014). Aptamer sensors for live-cell imaging of Pol II promoter activity. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14283

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ray, Judhajeet. “Aptamer sensors for live-cell imaging of Pol II promoter activity.” 2014. Thesis, Iowa State University. Accessed April 10, 2021. https://lib.dr.iastate.edu/etd/14283.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ray, Judhajeet. “Aptamer sensors for live-cell imaging of Pol II promoter activity.” 2014. Web. 10 Apr 2021.

Vancouver:

Ray J. Aptamer sensors for live-cell imaging of Pol II promoter activity. [Internet] [Thesis]. Iowa State University; 2014. [cited 2021 Apr 10]. Available from: https://lib.dr.iastate.edu/etd/14283.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ray J. Aptamer sensors for live-cell imaging of Pol II promoter activity. [Thesis]. Iowa State University; 2014. Available from: https://lib.dr.iastate.edu/etd/14283

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Technology, Sydney

14. Wood, MA. A novel approach to latent fingermark detection using aptamer-based reagents.

Degree: 2014, University of Technology, Sydney

URL: http://hdl.handle.net/10453/24200

► Research into latent fingermark detection and visualisation has taken many paths over the years as researchers and practitioners explore numerous methods to improve existing reagents.… (more)

▼ Research into latent fingermark detection and visualisation has taken many paths over the years as researchers and practitioners explore numerous methods to improve existing reagents. The majority of past research has resulted in providing small, incremental improvements to existing techniques. Currently, some researchers have opted to seek more transformational improvements in detection sensitivity, selectivity and visualisation. One such area being investigated is utilising immunology to target proteins, amino acids and drug metabolites in the latent fingermark deposit. Research to date has indicated that antibodies have great potential in providing these transformational improvements due to their ability to bind to certain fingermark components with high sensitivity and selectivity. Following on from the antibody research, aptamers have been highlighted as the next potential immunogenic technique for several reasons, including reduced health and safety issues, lower cost, greater sensitivity and selectivity, and ease of design and versatility. Aptamers are specifically selected oligonucleotides comprised of either ribonucleic acid (RNA) or single-stranded deoxyribonucleic acid (ssDNA). Due to the selection strategies employed, aptamers can be designed to target most molecules and bind to them with detection limits in the sub-micromolar to nanomolar ranges. Although aptamers have been successfully used in a variety of highly sensitive and selective detection devices, they have not been investigated for use in the detection and visualisation of latent fingermarks prior to this project. Initially, this project focussed on aptamers targeting amino acids as a means of visualising latent fingermarks. However, it was found that strong, non-specific interactions occurred with both the aptamer and the fluorescent tag, resulting in a lack of success with this approach. In order to address these issues, aptamers selected to the protein lysozyme were used on fingermarks placed on both PVDF and plain white copier paper. Lysozyme was selected as it was found to be a component in human sweat, while aptamers selected to lysozyme, with binding affinities in the nanomolar range were available. It was found that the aptamer-based reagents possessed high levels of sensitivity with the clear detection of lysozyme at very low concentrations (1 ng). Latent fingermarks from various donors were able to be detected on both substrates, with primary and secondary level detail being clearly visible. Results, however, were very inconsistent, with marks older than a couple of days being difficult to detect. This was found to be due to the degradation of lysozyme in the latent fingermark. Unfortunately, aptamers to other, possibly more suitable, fingermark components that would circumvent this problem were not available for this project. Despite the difficulties encountered, this project has, for the first time, demonstrated the potential of detecting and visualising latent fingermarks with an aptamer-based reagent. The study has laid the…

Subjects/Keywords: Fingermark.; Forensics.; Aptamer.; Detection.; Identification.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wood, M. (2014). A novel approach to latent fingermark detection using aptamer-based reagents. (Thesis). University of Technology, Sydney. Retrieved from http://hdl.handle.net/10453/24200

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wood, MA. “A novel approach to latent fingermark detection using aptamer-based reagents.” 2014. Thesis, University of Technology, Sydney. Accessed April 10, 2021. http://hdl.handle.net/10453/24200.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wood, MA. “A novel approach to latent fingermark detection using aptamer-based reagents.” 2014. Web. 10 Apr 2021.

Vancouver:

Wood M. A novel approach to latent fingermark detection using aptamer-based reagents. [Internet] [Thesis]. University of Technology, Sydney; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10453/24200.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wood M. A novel approach to latent fingermark detection using aptamer-based reagents. [Thesis]. University of Technology, Sydney; 2014. Available from: http://hdl.handle.net/10453/24200

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

15. Dunaway, Adam Blake. Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets.

Degree: MS, Materials Science and Engineering, 2014, Georgia Tech

URL: http://hdl.handle.net/1853/54310

► Deoxyribonucleic acid (DNA) aptamers are oligonucleotides with high specificity and affinity for non-nucleotide targets ranging from molecular species to cellular proteins. Their high affinity, rapid… (more)

Subjects/Keywords: DNA; Aptamer; Colloid; VEGF; Ampicillin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dunaway, A. B. (2014). Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/54310

Chicago Manual of Style (16^th Edition):

Dunaway, Adam Blake. “Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets.” 2014. Masters Thesis, Georgia Tech. Accessed April 10, 2021. http://hdl.handle.net/1853/54310.

MLA Handbook (7^th Edition):

Dunaway, Adam Blake. “Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets.” 2014. Web. 10 Apr 2021.

Vancouver:

Dunaway AB. Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets. [Internet] [Masters thesis]. Georgia Tech; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/1853/54310.

Council of Science Editors:

Dunaway AB. Characterization of the binding activity of immobilized DNA aptamers for nucleotide and non-nucleotide targets. [Masters Thesis]. Georgia Tech; 2014. Available from: http://hdl.handle.net/1853/54310

Texas State University – San Marcos

16. Navarro, Renato S. Click-Chemistry Based Molecularly Responsive Hydrogel as Biodegradable Scaffolds for 3-D Cell Culture: Design and Preparation of Building Blocks.

Degree: MS, Chemistry, 2014, Texas State University – San Marcos

URL: https://digital.library.txstate.edu/handle/10877/6898

► According to the American Cancer Society, every year over one million Americans are diagnosed with one of the recognized forms of cancer. Cancer metastasis is… (more)

▼ According to the American Cancer Society, every year over one million Americans are diagnosed with one of the recognized forms of cancer. Cancer metastasis is associated with poor patient prognosis. In order to better understand how cancer grows and metastasizes, researchers are interested in the development of platforms that could model the environment of tumor tissue and enable the study of the effect of cancer cell-secreted molecules on cell replication, extracellular matrix remodeling, and cell migration leading to metastasis. Due to their tissue-like properties, hydrogels have the ability to serve as such model systems by providing a three-dimensional scaffold in which cancerous cells could be cultured and studied. Hydrogels are formed from physically or chemically cross-linked hydrophilic polymers that form insoluble networks. Due to their high water content, hydrogels have mechanical properties that are similar to those of tissue; therefore, hydrogels could become ideal platforms for the study of cell growth and migration. The long-term goal of this work is to develop a hydrogel system that is molecularly responsive and can be synthesized at physiological conditions that could be used as a model the study of cancer cell growth. In order to achieve this goal, in this work we focused on: (1) the development of an aptamer complex to be used as crosslinker within the hydrogel network that is responsive towards the cancer cell-secreted protein vascular endothelium growth factor (VEGF), (2) the development of methods for the synthesis of hydrogels under mild conditions via copper-free click chemistry, and (3) the integration of cell adhesive properties to the hydrogels through a cyclo-Arginine-Glycine-Aspartic Acid (cRGD) peptide that mimics fibrin and collagen, some of the most abundant protein components of the extracellular matrix. The aptamer complex was designed to be able to hybridize a physiological conditions but denature in the presence of the molecular target VEGF, yielding a system that is responsive and degradable. Through the use of copper-free click chemistry, the hydrogels could be synthesized in the absence of toxins like free radicals, metals, and ultraviolet light that are typically used in polymerization reactions but that have deleterious effects on cells. The bioorthogonal reaction between azide and dibenzylcyclooctyne utilized in the copper-free click chemistry reaction between azide and dibenzylcyclooctyne utilized in the copper-free click chemistry reaction is able to rapidly link polymeric precursors, thus providing a method for immediate the encapsulation of cancer cells within the hydrogel scaffold. The encapsulated cells are then able to interact with the mimicry peptide cRGD and signal the cells to begin growing and proliferating. In doing so, a hydrogel that is molecular responsive, nontoxic, and capable of encapsulating cells is fashioned in order to serve as three-dimensional platform for their study. Chapter 1 provides a background on the various concepts on which the proposed… Advisors/Committee Members: Betancourt, Tania (advisor), Corina, Maeder (committee member), Brittain, William (committee member).

Subjects/Keywords: Biomaterials; Cell culture; Aptamer

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APA (6^th Edition):

Navarro, R. S. (2014). Click-Chemistry Based Molecularly Responsive Hydrogel as Biodegradable Scaffolds for 3-D Cell Culture: Design and Preparation of Building Blocks. (Masters Thesis). Texas State University – San Marcos. Retrieved from https://digital.library.txstate.edu/handle/10877/6898

Chicago Manual of Style (16^th Edition):

Navarro, Renato S. “Click-Chemistry Based Molecularly Responsive Hydrogel as Biodegradable Scaffolds for 3-D Cell Culture: Design and Preparation of Building Blocks.” 2014. Masters Thesis, Texas State University – San Marcos. Accessed April 10, 2021. https://digital.library.txstate.edu/handle/10877/6898.

MLA Handbook (7^th Edition):

Navarro, Renato S. “Click-Chemistry Based Molecularly Responsive Hydrogel as Biodegradable Scaffolds for 3-D Cell Culture: Design and Preparation of Building Blocks.” 2014. Web. 10 Apr 2021.

Vancouver:

Navarro RS. Click-Chemistry Based Molecularly Responsive Hydrogel as Biodegradable Scaffolds for 3-D Cell Culture: Design and Preparation of Building Blocks. [Internet] [Masters thesis]. Texas State University – San Marcos; 2014. [cited 2021 Apr 10]. Available from: https://digital.library.txstate.edu/handle/10877/6898.

Council of Science Editors:

Navarro RS. Click-Chemistry Based Molecularly Responsive Hydrogel as Biodegradable Scaffolds for 3-D Cell Culture: Design and Preparation of Building Blocks. [Masters Thesis]. Texas State University – San Marcos; 2014. Available from: https://digital.library.txstate.edu/handle/10877/6898

Boise State University

17. Burden, Steven J. The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals.

Degree: 2019, Boise State University

URL: https://scholarworks.boisestate.edu/td/1627

► Fluorogenic aptamers are emerging as useful molecular tools to track RNA molecules in cells and as a platform for fluorescent biosensors. The recently developed RNA… (more)

Subjects/Keywords: RNA; aptamer; fluorogenic aptamer; Other Biochemistry, Biophysics, and Structural Biology

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APA (6^th Edition):

Burden, S. J. (2019). The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals. (Thesis). Boise State University. Retrieved from https://scholarworks.boisestate.edu/td/1627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burden, Steven J. “The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals.” 2019. Thesis, Boise State University. Accessed April 10, 2021. https://scholarworks.boisestate.edu/td/1627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burden, Steven J. “The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals.” 2019. Web. 10 Apr 2021.

Vancouver:

Burden SJ. The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals. [Internet] [Thesis]. Boise State University; 2019. [cited 2021 Apr 10]. Available from: https://scholarworks.boisestate.edu/td/1627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burden SJ. The Development of Nucleic Acid Biosensors with Allosteric Fluorescence Signals. [Thesis]. Boise State University; 2019. Available from: https://scholarworks.boisestate.edu/td/1627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Marina Ribeiro Batistuti. Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers.

Degree: 2017, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/59/59135/tde-26072017-141445/

►

The electrochemical biosensor has been extensively used due to its capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers.… (more)

▼

The electrochemical biosensor has been extensively used due to its capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers. DNA hybridization sensors are based on the increase of negative charge on the electrode surface after the DNA target hybridize to the immobilized probes. The development of this platform requires first an understanding of the immobilization process and optimization of surface probe density. In this thesis the electron transfer is investigated on a label-free DNA hybridization detection by its intrinsic charge. The investigation using different immobilization buffers shows a strong dependence on their composition and concentration, and also the influence of the probe and spacer co-immobilized to obtain an organized and compact self-assembled monolayer. The probe density is determined using the chronocoulometry method with hexaammineruthenium (III) chloride, where the value is calculated from the number of cationic redox molecules electrostatically associated with the anionic DNA backbone and presented a linear relationship between thiol molar fraction and probe density from 2 to 5 x 1012 molecules/cm2. The effect of hybridization was determined using electrochemical impedance spectroscopy using negatively charged ferri/ferrocyanide redox couple in solution. After probe surface density optimization, the maximum shift of charge transfer resistence (20%) upon 1 M complementary sequence was obtained with around 25% probe fraction immobilized vii viii on surface. This electrochemical platform developed was able to detected 100 pM of target sequence and distinguish mismatched sequences. The limit of detection is higher when compared to the literature, however, this system can be further improved by amplifing the signal. The same platform is reproduced in the quartz crystal microbalance system and with field-effect transistor, comparing the different detections. The same platform is tested using two different HER2 aptamer sequences. Biological aspects are explored for a better understanding of the system

O biossensor eletroquímico foi amplamente utilizado devido à sua capacidade de Detecção rápida e precisa de uma grande variedade de moléculas alvo ou biomarcadores. Os sensores de hibridização do DNA são baseados no aumento da carga negativa na Superfície do eletrodo depois que o alvo do DNA se hibridar com as sondas imobilizadas. o O desenvolvimento desta plataforma exige primeiro uma compreensão da imobilização Processo e otimização da densidade da sonda de superfície. Nesta tese, o elétron A transferência é investigada em uma detecção de hibridação de DNA sem rótulas por sua intrínseca carregar. A investigação usando diferentes buffers de imobilização mostra uma forte Dependência de sua composição e concentração, e também a influência da Sonda e espaçador co-imobilizados para obter uma auto-montagem organizada e compacta Monocamada. A densidade da sonda é determinada usando o método de cronoculometria Com cloreto de hexaamminerutenio (III), onde o…

Advisors/Committee Members: Marcelo Mulato, Paulo Roberto Bueno, Carlos César Bof' Bufon, Emanuel Carrilho, Pedro Miguel de Lemos Correia Estrela, Lauro Tatsuo Kubota.

Subjects/Keywords: Aptamer; DNA; Electrochemical; QCM; Aptamer; DNA; Eletroquímica; QCM

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APA (6^th Edition):

Batistuti, M. R. (2017). Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/59/59135/tde-26072017-141445/

Chicago Manual of Style (16^th Edition):

Batistuti, Marina Ribeiro. “Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers.” 2017. Doctoral Dissertation, University of São Paulo. Accessed April 10, 2021. http://www.teses.usp.br/teses/disponiveis/59/59135/tde-26072017-141445/.

MLA Handbook (7^th Edition):

Batistuti, Marina Ribeiro. “Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers.” 2017. Web. 10 Apr 2021.

Vancouver:

Batistuti MR. Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers. [Internet] [Doctoral dissertation]. University of São Paulo; 2017. [cited 2021 Apr 10]. Available from: http://www.teses.usp.br/teses/disponiveis/59/59135/tde-26072017-141445/.

Council of Science Editors:

Batistuti MR. Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers. [Doctoral Dissertation]. University of São Paulo; 2017. Available from: http://www.teses.usp.br/teses/disponiveis/59/59135/tde-26072017-141445/

University of California – Berkeley

19. Cho, Hansang. Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors.

Degree: Bioengineering, 2010, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/23684207

► Recent advances in micro/nano- technologies have shown high potentials in the field of quantitative biology, biomedical science, and analytical chemistry. However, micro/nano fluidics still requires… (more)

▼ Recent advances in micro/nano- technologies have shown high potentials in the field of quantitative biology, biomedical science, and analytical chemistry. However, micro/nano fluidics still requires multi-layered structures, complex plumbing/tubing, and external equipments for large-scale applications and nanotechnology-based sensors demand high cost. Interestingly, nature has much simpler and more effective solutions. The goal of this dissertation is to develop novel microfluidic platforms and nanobiosensors inspired by biological systems.In this dissertation, I report the development of a biologically inspired bidirectional fluidic diode motivated by the xylem pores, which allows designing a functional large-scale microfluidic circuit and autonomous fluidic controls without any delegate efforts on fluid regulation. The biologically inspired bidirectional fluidic diode requires only a single-layered structure and a single pressure source to regulate flow in both directions through the entire platform. The operational conditions are precisely estimated based on the fully developed analytical model, which considers the hysteresis of contact angles and effects of fabrication limitations. To demonstrate its many possible applications, I show large-scale, spontaneous droplet-patterning and colonized cell-patterning programmed with the uni- and bi-directional fluidic diodes in the microfludic platform. In addition, inspired by target recognition in nature, an aptamer-based nanoplasmonic sensor, `aptasensor' is presented by detecting a coagulation protein, human α-thrombin. Also, I present an aptasensor targeting vascular endothelial growth factor-165 (VEGF165), a predominant and effective cancer biomarker for the diagnostics of various solid cancers. The integration of an aptasensor into a microfluidic platform is demonstrated by applying the VEGF165 aptasensor to detect secreted VEGF165 from breast cancer cells cultured in a microfluidic platform.I envision that elucidation of mechanisms underlying in biological systems will inspire to create new technologies for the application to precision biology, biotechnology, and medicine. Furthermore, the developed biologically inspired engineering will provide physical insights, analytical models, and useful tools for the better understanding of biological systems.

Subjects/Keywords: Biomedical engineering; aptamer; bioinspiration; microfluidics; plasmonics; sensor

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APA (6^th Edition):

Cho, H. (2010). Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/23684207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cho, Hansang. “Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors.” 2010. Thesis, University of California – Berkeley. Accessed April 10, 2021. http://www.escholarship.org/uc/item/23684207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cho, Hansang. “Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors.” 2010. Web. 10 Apr 2021.

Vancouver:

Cho H. Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors. [Internet] [Thesis]. University of California – Berkeley; 2010. [cited 2021 Apr 10]. Available from: http://www.escholarship.org/uc/item/23684207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cho H. Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors. [Thesis]. University of California – Berkeley; 2010. Available from: http://www.escholarship.org/uc/item/23684207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

20. Meng, Hsien-Wei. Development Of Dna Aptamers By Cell-Selex Using Yeast Cell Surface Display.

Degree: PhD, Veterinary Medicine, 2014, Cornell University

URL: http://hdl.handle.net/1813/36083

► SELEX, the process of selecting aptamers, is often hampered by the difficulty of preparing target molecules in their native forms and by a lack of… (more)

Subjects/Keywords: Aptamer; Cell-SELEX; Yeast Surface Display

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APA (6^th Edition):

Meng, H. (2014). Development Of Dna Aptamers By Cell-Selex Using Yeast Cell Surface Display. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/36083

Chicago Manual of Style (16^th Edition):

Meng, Hsien-Wei. “Development Of Dna Aptamers By Cell-Selex Using Yeast Cell Surface Display.” 2014. Doctoral Dissertation, Cornell University. Accessed April 10, 2021. http://hdl.handle.net/1813/36083.

MLA Handbook (7^th Edition):

Meng, Hsien-Wei. “Development Of Dna Aptamers By Cell-Selex Using Yeast Cell Surface Display.” 2014. Web. 10 Apr 2021.

Vancouver:

Meng H. Development Of Dna Aptamers By Cell-Selex Using Yeast Cell Surface Display. [Internet] [Doctoral dissertation]. Cornell University; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/1813/36083.

Council of Science Editors:

Meng H. Development Of Dna Aptamers By Cell-Selex Using Yeast Cell Surface Display. [Doctoral Dissertation]. Cornell University; 2014. Available from: http://hdl.handle.net/1813/36083

McMaster University

21. Cabrera, Pablo. CALF INTESTINAL ALKALINE PHOSPHATASE APTAMER BASED BIOSENSORS.

Degree: MASc, 2014, McMaster University

URL: http://hdl.handle.net/11375/16431

►

In recent years, there has been an increasing demand for newer, more accurate, technologies that can detect and identify biomolecules or biological entities related to… (more)

▼

In recent years, there has been an increasing demand for newer, more accurate, technologies that can detect and identify biomolecules or biological entities related to health, agriculture or the environment. With the discovery of new properties of nucleic acids beyond the storage and transfer of genetic information, a new class of nucleic acid-based biosensors is emerging, using DNA and RNA as target recognition elements with the advantage of being simpler and more cost-effective compared to antibodies-based biosensor. Two sequences, TrG14MC and TrG10SC, with evidence to suggest that they are capable of inhibit the metalloenzyme CIP, were isolated from a selection conducted by Dr. Razvan Nutiu. Here we study the inhibitory properties of these two aptamer candidates and measure the IC50 value, determined as 94 nM for TrG14MC and 83 nM for TrG10SC. Different bivalent constructs, designed to increase the inhibitory effect of the isolated sequences, are studied showing a pronounce influence of the linker length improving the inhibitory effect over CIP. Modulating the interaction of the isolated sequences and the CIP is of key importance in order to develop a successful biosensor. Therefore, we try to recover CIP from the inhibition effect by using antisense sequences complementary to different segments of the construct. The maximum recovery, 75%, was achieved by an antisense sequence fully complemented to the inhibitory bivalent construct. We also study here the use of a linker in the bivalent construct that forms a secondary hairpin structure, and the effect of linearizing that structure with an antisense sequence complementary to the linker. This resulted in as 12% of the inhibitory effect. The purpose of this investigation was to establish the first steps toward the development of a new class of biosensors capable of disinhibiting CIP upon the recognition of a specific target, taking advantage of the suggested CIP-inhibitory properties of the isolated sequences TrG14MC and TrG10SC.

Thesis

Master of Applied Science (MASc)

Advisors/Committee Members: Filipe, Carlos, Chemical Engineering.

Subjects/Keywords: aptamer; selex; Calf intestinal phosphatase; biosensor

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APA (6^th Edition):

Cabrera, P. (2014). CALF INTESTINAL ALKALINE PHOSPHATASE APTAMER BASED BIOSENSORS. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/16431

Chicago Manual of Style (16^th Edition):

Cabrera, Pablo. “CALF INTESTINAL ALKALINE PHOSPHATASE APTAMER BASED BIOSENSORS.” 2014. Masters Thesis, McMaster University. Accessed April 10, 2021. http://hdl.handle.net/11375/16431.

MLA Handbook (7^th Edition):

Cabrera, Pablo. “CALF INTESTINAL ALKALINE PHOSPHATASE APTAMER BASED BIOSENSORS.” 2014. Web. 10 Apr 2021.

Vancouver:

Cabrera P. CALF INTESTINAL ALKALINE PHOSPHATASE APTAMER BASED BIOSENSORS. [Internet] [Masters thesis]. McMaster University; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/11375/16431.

Council of Science Editors:

Cabrera P. CALF INTESTINAL ALKALINE PHOSPHATASE APTAMER BASED BIOSENSORS. [Masters Thesis]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16431

McMaster University

22. Tram, Kha. EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT.

Degree: PhD, 2015, McMaster University

URL: http://hdl.handle.net/11375/16884

►

The development of the in vitro selection technique permits the creation of synthetic DNA molecules with ligand-binding capabilities (DNA aptamers), or abilities to catalyze chemical… (more)

Subjects/Keywords: DNAzyme; Aptamer; Biosensors; Functional Nucleic Acids

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APA (6^th Edition):

Tram, K. (2015). EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16884

Chicago Manual of Style (16^th Edition):

Tram, Kha. “EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT.” 2015. Doctoral Dissertation, McMaster University. Accessed April 10, 2021. http://hdl.handle.net/11375/16884.

MLA Handbook (7^th Edition):

Tram, Kha. “EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT.” 2015. Web. 10 Apr 2021.

Vancouver:

Tram K. EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT. [Internet] [Doctoral dissertation]. McMaster University; 2015. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/11375/16884.

Council of Science Editors:

Tram K. EXPLORING SYNTHETIC FUNCTIONAL DNA MOLECULES FOR BIOSENSOR DEVELOPMENT. [Doctoral Dissertation]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/16884

Virginia Commonwealth University

23. Zhang, Xiaojuan. INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS.

Degree: PhD, Engineering, 2011, Virginia Commonwealth University

URL: https://doi.org/10.25772/ZE28-2X20 ; https://scholarscompass.vcu.edu/etd/294

► The highly specific recognition processes between biomolecules mediate various crucial biological processes. Uncovering the molecular basis of these interactions is of great fundamental and applied… (more)

▼ The highly specific recognition processes between biomolecules mediate various crucial biological processes. Uncovering the molecular basis of these interactions is of great fundamental and applied importance. This research work focuses on understanding the interactions of several biomolecular recognition systems and processes that can provide fundamental information to aid in the rational design of sensing and molecular recognition tools. Initially, a reliable and versatile platform was developed to investigate biomolecular interactions at a molecular level. This involved several techniques, including biomolecule functionalization to enable attachment to self-assembled monolayers as well as atomic force microscopy (AFM) based force spectroscopy to uncover the binding or rupture forces between the receptor and ligand pairs. It was shown that this platform allowed determination of molecular binding between single molecules with a high specificity. The platform was further adapted to a general sensing formulation utilizing a group of flexible and adaptive nucleic acid recognition elements (RNA and DNA aptamers) to detect specific target proteins. Investigation of interactions at the molecular level allowed characterization of the dynamics, specificity and the conformational properties of these functional nucleic acids in a manner inaccessible via traditional interaction studies. These interactions were then adapted to aptamer-based detecting methods that at the ensemble or bulk scale, specifically taking advantage of mechanisms uncovered in the biophysical study of this system. A quartz crystal microbalance (QCM) was used to detect protein targets at the bulk level and the affinities and binding kinetics of these systems were analyzed. Along with AFM-based force spectroscopy, ensemble-averaging properties and molecular properties of these interactions could be correlated to contribute to bridging the gap across length scales. Finally, more broadly applicable sensing platform was developed to take advantage of the unique properties of aptamers. DNA was employed both as a carrier and as a molecular recognition agent. DNA was used as a template for nanoconstruction and fabricating unique shapes that could enhance the aptamer-based molecular recognition strategies. With aptamers tagged to distinct nanoconstructed DNA, a novel shape-based detecting method was enabled at the molecular level. The results demonstrated that this is a flexible strategy, which can be further developed as ultrasensitive single molecule sensing strategy in complex environments. Advisors/Committee Members: Vamsi K. Yadavalli.

Subjects/Keywords: AFM; Biomolecular interaction; Biosensing; Aptamer; Engineering

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APA (6^th Edition):

Zhang, X. (2011). INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/ZE28-2X20 ; https://scholarscompass.vcu.edu/etd/294

Chicago Manual of Style (16^th Edition):

Zhang, Xiaojuan. “INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS.” 2011. Doctoral Dissertation, Virginia Commonwealth University. Accessed April 10, 2021. https://doi.org/10.25772/ZE28-2X20 ; https://scholarscompass.vcu.edu/etd/294.

MLA Handbook (7^th Edition):

Zhang, Xiaojuan. “INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS.” 2011. Web. 10 Apr 2021.

Vancouver:

Zhang X. INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2011. [cited 2021 Apr 10]. Available from: https://doi.org/10.25772/ZE28-2X20 ; https://scholarscompass.vcu.edu/etd/294.

Council of Science Editors:

Zhang X. INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS. [Doctoral Dissertation]. Virginia Commonwealth University; 2011. Available from: https://doi.org/10.25772/ZE28-2X20 ; https://scholarscompass.vcu.edu/etd/294

NSYSU

24. Yin, Yao-De. The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin.

Degree: Master, Institute of Biomedical Sciences, 2018, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541

► Aptamers are single-stranded DNA or RNA fragments that bind to targeted molecules via folding into specific structure. Thus, aptamers can be employed as aptasensors for… (more)

▼ Aptamers are single-stranded DNA or RNA fragments that bind to targeted molecules via folding into specific structure. Thus, aptamers can be employed as aptasensors for detecting targeted molecules. The aim of this study was to investigate the utility of DNA aptamers against Bungarus multicinctus (Taiwan banded krait) presynaptic neurotoxin Î²-bungarotoxin (Î²-Bgt) as biosensors. Four Î²-Bgt aptamers, Bgt1, Bgt2, Bgt3, and Bgt4 were synthesized according to previously published results. Electrophoretic mobility shift assay showed that Î²-Bgt reduced the electrophoretic mobility of the aptamers. Moreover, Î²-Bgt reduced fluorescence intensity of the aptamers labeled with 5'-end carboxyfluorescein (FAM) and 3'-end 4-([4-(dimethylamino)-phenyl]azo)-benzoic acid (Dabcyl). These results showed that the folded structure of aptamers changed upon binding with Î²-Bgt. Measurement of the changes in FAM fluorescence revealed that Î²-Bgt had the highest binding affinity for Bgt1 among the four aptamers. In contrast to palmatine and berberine, coralyne drastically reduced FAM fluorescence intensity of 5'-FAM-Bgt1 and 5'-FAM-Bgt1-3'-Dabcyl, suggesting the selectivity of the sensors for detecting coralyne. The limit of detection (LOD) of coralyne determined by 5'-FAM-Bgt1 and 5'-FAM-Bgt1-3'-Dabcyl were 0.034 Î¼M and 0.021 Î¼M, respectively. Furthermore, coralyne-5'-FAM-Bgt1 and coralyne-5'-FAM-Bgt1-3'-Dabcyl complexes were employed as turn-on fluorescent sensors for detecting Î²-Bgt and heparin. Î²-Bgt and heparin notably restored FAM fluorescence intensity of coralyne-5'-FAM-Bgt1 and coralyne-5'-FAM-Bgt1-3'-Dabcyl complexes. The LOD of Î²-Bgt measured by coralyne-5'-FAM-Bgt1 and coralyne-5'-FAM-Bgt1-3'-Dabcyl complexes were 3.57 nM and 2.74 nM, respectively; the LOD of heparin were 0.012 Î¼g/ml and 0.004 Î¼g/ml, respectively; and the LOD for heparin-spiked serum were 0.027 Î¼g/ml and 0.009 Î¼g/ml, respectively. The sensor system constructed by coralyne and Bgt1 showed a high selectivity for heparin detection than for other glycosaminoglycans including chondroitin sulfate and hyaluronic acid. Although the sensor system effectively differentiated Î²-Bgt from other purified snake venom proteins, it could not specifically detect Taiwan banded krait crude venom. Taken together, our data indicate that aptamer against Î²-Bgt can be used for constructing biosensors for detecting coralyne and heparin. However, the sensor system is unable to identify the crude venoms of different snake species via Î²-Bgt detection. Advisors/Committee Members: Bin-Nan Wu (chair), Long-Sen Chang (committee member), Ying-Jung Chen (chair).

Subjects/Keywords: DNA aptamer; Sensors; Heparin; Coralyne; Î²-Bungarotoxin

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APA (6^th Edition):

Yin, Y. (2018). The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yin, Yao-De. “The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin.” 2018. Thesis, NSYSU. Accessed April 10, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yin, Yao-De. “The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin.” 2018. Web. 10 Apr 2021.

Vancouver:

Yin Y. The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin. [Internet] [Thesis]. NSYSU; 2018. [cited 2021 Apr 10]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yin Y. The Utility of DNA Aptamers against Î²-Bungarotoxin as Sensors for Detecting Î²-Bungarotoxin, Coralyne and Heparin. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0528118-084541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Rovira i Virgili

25. Nadal Polo, Pedro. Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1.

Degree: Departament d'Enginyeria Química, 2012, Universitat Rovira i Virgili

URL: http://hdl.handle.net/10803/84036

► Lupin has recently been added to the list of allergens requiring mandatory advisory labelling on foodstuffs sold in the European Union, and since December 2008… (more)

▼ Lupin has recently been added to the list of allergens requiring mandatory advisory labelling on foodstuffs sold in the European Union, and since December 2008 all products containing even trace amounts of lupin must be labelled correctly. Lupin globulins consist of two major globulins called α-conglutin (11S and “legumin-like”) and β-conglutin (7S and “vicilin-like”), and another additional two globulins, γ-conglutin and δ-conglutin, which are present in lower amounts. β-conglutin is the only conglutin currently included in the list of the International Union of Immunological Societies (IUIS), designated as Lup an 1. The overall objective of these PhD is the selection of aptamers that can detect this allergen. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. Aptamers possess unique chemical and biochemical characteristics, such as: well known chemistry and remarkable stability, moreover, aptamers can be selected against virtually any target and in non-physiological conditions. In order to achieve the overall objective, a set of subobjectives will be achieved. The first of these involves the elucidation of protocols for the selective extraction of each of the lupin α, β, γ, and δ subunits, resulting in (i) protocols that can be used for selective extraction and isolation of the lupin α, β, γ, and δ proteins from food for subsequent analysis; (ii) standards that can be used in analytical assays and tools; and (iii) target that can be used for the selection of aptamers specific to the β-conglutin subunit. The core of the work is the selection of aptamers against the allergen Lup an 1 using a SELEX procedure, as well the preparation of protocols that can be used to monitor the evolution of aptamer selection. The functionality of the aptamer is demonstratedby exploiting it in an enzyme linked oligonucleotide assay as well as apta-PCR. Finally the resulting aptamer candidates that exhibit high affinity are fully characterised, truncated, and the structure of the final truncated aptamer is elucidated Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), O'Sullivan, Ciara (director), true (authorsendemail).

Subjects/Keywords: Aptamer; Selex; Lupin; 577; 663/664

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APA (6^th Edition):

Nadal Polo, P. (2012). Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1. (Thesis). Universitat Rovira i Virgili. Retrieved from http://hdl.handle.net/10803/84036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nadal Polo, Pedro. “Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1.” 2012. Thesis, Universitat Rovira i Virgili. Accessed April 10, 2021. http://hdl.handle.net/10803/84036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nadal Polo, Pedro. “Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1.” 2012. Web. 10 Apr 2021.

Vancouver:

Nadal Polo P. Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1. [Internet] [Thesis]. Universitat Rovira i Virgili; 2012. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/10803/84036.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nadal Polo P. Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1. [Thesis]. Universitat Rovira i Virgili; 2012. Available from: http://hdl.handle.net/10803/84036

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Francisco

26. Kundert, Kale. Designing functional macromolecules.

Degree: Biophysics, 2018, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/61f0f866

► Biology is driven by functional macromolecules, most notably proteins and non-coding RNAs. Learning how to design similarly functional macromolecules is a natural goal. Success will… (more)

Subjects/Keywords: Biophysics; aptamer; design; loop; protein; sgRNA

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APA (6^th Edition):

Kundert, K. (2018). Designing functional macromolecules. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/61f0f866

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kundert, Kale. “Designing functional macromolecules.” 2018. Thesis, University of California – San Francisco. Accessed April 10, 2021. http://www.escholarship.org/uc/item/61f0f866.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kundert, Kale. “Designing functional macromolecules.” 2018. Web. 10 Apr 2021.

Vancouver:

Kundert K. Designing functional macromolecules. [Internet] [Thesis]. University of California – San Francisco; 2018. [cited 2021 Apr 10]. Available from: http://www.escholarship.org/uc/item/61f0f866.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kundert K. Designing functional macromolecules. [Thesis]. University of California – San Francisco; 2018. Available from: http://www.escholarship.org/uc/item/61f0f866

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

27. Kasturiarachchi, Jagath Chandana. Bio-oligomers as antibacterial agents and strategies for bacterial detection.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/10030

► In this thesis I examined the potential of Bio-Oligomers such as peptoids, peptides and aptamers, as therapeutic and diagnostic entities. Therapeutic Bio-Oligomers; A series of… (more)

▼ In this thesis I examined the potential of Bio-Oligomers such as peptoids, peptides and aptamers, as therapeutic and diagnostic entities. Therapeutic Bio-Oligomers; A series of peptoid analogs have been designed and synthesised using solid phase synthesis. These peptoids have been subjected to biological evaluation to determine structure-activity relationships that define their antimicrobial activity. In total 13 peptoids were synthesised. Out of 13 different peptoids, only one peptoid called Tosyl-Octyl-Peptoid (TOP) demonstrated significant broad-spectrum bactericidal activity. TOP kills bacteria under non-dividing and dividing conditions. The Minimum Inhibitory Concentrations (MIC) values of TOP for S. epidermidis, E. coli and Klebsiella were 20 μM, whereas Methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-sensitive Staphylococcus aureus (MSSA) were 40 μM. The highest MIC values were observed for Pseudomonas aeruginosa (PAO1) at 80 μM. The selectivity ratio (SR) or Therapeutic index (TI) was calculated, by dividing the 10% haemolysis activity (5 mM) by the median of the MIC (50 μM) yielding a TI for TOP as 100. This TI is well above previously reported peptidomimetics TI of around 20. TOP demonstrates selective bacterial killing in co-culture systems and intracellular bacterial killing activity. Diagnostic Bio-Oligomers; In the second part of my thesis, I investigated aptamer and peptide-based molecular probes to detect MRSA. As well as screening aptamers and peptide probes against whole MRSA, I over-expressed and purified PBP2A protein. This purified protein was used as a target for aptamer and peptide probes to detect MRSA. Two different aptamer libraries were initially screened for utility. In-vitro conditions for SELEX were optimised. Biopanning with a phage derived peptides was also performed. Target sequences for both methods were identified and chemically synthesised. Evaluation of fluorescently labelled sequences with flow cytometry and confocal imaging showed no specificity for MRSA detection with either method. The Bio-Oligomers and the in-vitro selection methodology require further refinement to improve diagnostic utility.

Subjects/Keywords: 616.9; antibacterial drugs; imaging; aptamer; peptide; peptoid

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APA (6^th Edition):

Kasturiarachchi, J. C. (2014). Bio-oligomers as antibacterial agents and strategies for bacterial detection. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/10030

Chicago Manual of Style (16^th Edition):

Kasturiarachchi, Jagath Chandana. “Bio-oligomers as antibacterial agents and strategies for bacterial detection.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed April 10, 2021. http://hdl.handle.net/1842/10030.

MLA Handbook (7^th Edition):

Kasturiarachchi, Jagath Chandana. “Bio-oligomers as antibacterial agents and strategies for bacterial detection.” 2014. Web. 10 Apr 2021.

Vancouver:

Kasturiarachchi JC. Bio-oligomers as antibacterial agents and strategies for bacterial detection. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/1842/10030.

Council of Science Editors:

Kasturiarachchi JC. Bio-oligomers as antibacterial agents and strategies for bacterial detection. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/10030

Iowa State University

28. Ilgu, Muslum. Deciphering the Details of RNA Aminoglycoside Interactions: From Atomistic Models to Biotechnological Applications.

Degree: 2012, Iowa State University

URL: https://lib.dr.iastate.edu/etd/12750

► Aminoglycosides are a class of antibiotics functioning through binding to 16S rRNA A-site and inhibiting the bacterial translation. However, the continuous emergence of drug-resistant strains… (more)

▼ Aminoglycosides are a class of antibiotics functioning through binding to 16S rRNA A-site and inhibiting the bacterial translation. However, the continuous emergence of drug-resistant strains makes the development of new and more potent antibiotics necessary. Aminoglycosides are also known to interact with various biologically crucial RNA molecules other than 16S rRNA A-site and inhibit their functions. As a result, they are considered as the single most important model to understand the principles of RNA small molecule recognition. The detailed understanding of these interactions is necessary for the development of novel antibacterial, antiviral or even anti-oncogenic agents. In our studies, we have studied both the natural aminoglycoside targets like Rev responsive element (RRE), trans-activating region (TAR) of HIV-1 and thymidylate synthase mRNA 5' untranslated (UTR) region as well as the in vitro selected neomycin, tobramycin and kanamycin RNA aptamers. By this way, we think we have covered a variety of binding pockets to figure out the critical nucleic acid residues playing essential role in aminoglycoside recognition. Along with all these RNAs, we studied more than 10 aminoglycoside ligands to pinpoint the chemical groups in close contact with RNAs. To determine thermodynamic parameters for these interactions, we utilized isothermal titration calorimetry (ITC) assay by which we found that the majority of these interactions are enthalpy driven. More specifically, RNA aminoglycoside interactions are mainly derived by electrostatic and hydrogen binding interactions. Our studies indicated that the amino groups on the first ring of the aminoglycosides are essential for high affinity binding whereas having bulky groups on ring II sterically eliminate their interactions with RNAs. RNA binding trend of aminoglycosides are as follows: neomycin-B > ribostamycin > kanamycin-B > tobramycin > paromomycin > sisomicin > gentamicin > kanamycin-A > geneticin > amikacin > netilmicin. Aminoglycoside binding to the aptamer was shown highly buffer dependent. This phenomenon was analyzed in five different buffers and found that cacodylate-based buffer changes the specificity of the aptamer. In addition to ITC, we have used molecular docking to specifically find out the chemical groups in these interactions. We have specified the nucleic acid residues interacting with aminoglycosides. In parallel, molecular dynamics (MD) simulations of neomycin RNA aptamer with neomycin-B in an all-atom platform in GROMACS were carried out. The results showed a mobile structure consistent with the ability of this aptamer to interact with a wide range of ligands. From molecular docking and MD simulations, we identified the neomycin-B aptamer residues that might contribute to its ligand selectivity and designed a series of new aptamers accordingly. Also, A16 was found to be flexible, which was confirmed by 2AP fluorescence studies. In this analysis, the buffer dependence was also confirmed against neomycin-B, ribostamycin and paromomycin. One of the…

Subjects/Keywords: Aminoglycoside; ITC; RNA Aptamer; RNA imaging; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ilgu, M. (2012). Deciphering the Details of RNA Aminoglycoside Interactions: From Atomistic Models to Biotechnological Applications. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/12750

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ilgu, Muslum. “Deciphering the Details of RNA Aminoglycoside Interactions: From Atomistic Models to Biotechnological Applications.” 2012. Thesis, Iowa State University. Accessed April 10, 2021. https://lib.dr.iastate.edu/etd/12750.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ilgu, Muslum. “Deciphering the Details of RNA Aminoglycoside Interactions: From Atomistic Models to Biotechnological Applications.” 2012. Web. 10 Apr 2021.

Vancouver:

Ilgu M. Deciphering the Details of RNA Aminoglycoside Interactions: From Atomistic Models to Biotechnological Applications. [Internet] [Thesis]. Iowa State University; 2012. [cited 2021 Apr 10]. Available from: https://lib.dr.iastate.edu/etd/12750.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ilgu M. Deciphering the Details of RNA Aminoglycoside Interactions: From Atomistic Models to Biotechnological Applications. [Thesis]. Iowa State University; 2012. Available from: https://lib.dr.iastate.edu/etd/12750

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

29. Zhu, Zhichen. Detection techniques for molecular interactions based on interferometry and micro-cantilever.

Degree: 2018, Iowa State University

URL: https://lib.dr.iastate.edu/etd/17379

► Biosensors based on Atomic force microscope (AFM) cantilevers have recently been showing promising potential because of its portability and high sensitivity, the gold coated cantilever… (more)

▼ Biosensors based on Atomic force microscope (AFM) cantilevers have recently been showing promising potential because of its portability and high sensitivity, the gold coated cantilever surface allows functionalization on the cantilever by immobilizing biomolecules like enzyme, DNA, RNA on the cantilever surface. Biomolecular reactions between the immobilized molecules and its target molecules on the cantilever can result in change in measurable mechanical signal like deflection, resonant frequency. Researchers can detect the presence and quantity of target molecules by measuring the deflections or resonant frequency change. This property of micro cantilever allows itself to have potential application in DNA/Protein detection. In addition, the gold surface of cantilever makes it possible to apply electrochemical technology in the biosensing system. We designed a mini optical system to monitor the deflection of cantilever when the DNA aptamer of Ebola virus glycoprotein (GP1,2) that is immobilized on the cantilever reacts with GP1,2 in the environment. The same experiment was conducted using Ebola secreted glycoprotein (sGP) and its DNA aptamer. The results show that the increase of GP1,2 concentration led to an increase in tensile surface stress, the tensile surface stress saturated at about 200nM of GP1,2. While the sGP results show that the increase of sGP concentration produced a compressive surface stress which saturates at 20nM. The linear range of experiment data has potential in the detection of Ebola virus at nanomolar concentration. We also set up a micro optical system of shoe box size to monitor the deflection of cantilever when the immobilized aptamers bind with target molecules in the environment. A verification with human alpha thrombin and its aptamer was conducted, which shows a promising potential of alpha thrombin detection. The portability of this new system allows a rapid detection at point-of-care. Other future work is planned in the last chapter, which includes a molecular dynamics simulation of stress induced by the binding between glycoprotein and its aptamer, and a novel electrochemical biosensor based on micro-cantilever.

Subjects/Keywords: Aptamer; Biosensor; Ebola; Interferometer; Microcantilever; Mechanical Engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhu, Z. (2018). Detection techniques for molecular interactions based on interferometry and micro-cantilever. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/17379

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhu, Zhichen. “Detection techniques for molecular interactions based on interferometry and micro-cantilever.” 2018. Thesis, Iowa State University. Accessed April 10, 2021. https://lib.dr.iastate.edu/etd/17379.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhu, Zhichen. “Detection techniques for molecular interactions based on interferometry and micro-cantilever.” 2018. Web. 10 Apr 2021.

Vancouver:

Zhu Z. Detection techniques for molecular interactions based on interferometry and micro-cantilever. [Internet] [Thesis]. Iowa State University; 2018. [cited 2021 Apr 10]. Available from: https://lib.dr.iastate.edu/etd/17379.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhu Z. Detection techniques for molecular interactions based on interferometry and micro-cantilever. [Thesis]. Iowa State University; 2018. Available from: https://lib.dr.iastate.edu/etd/17379

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Fomo, Gertrude. Ionophoric and aptameric recognition-modulated electroactive polyaniline films for the determination of tetrodotoxin .

Degree: 2014, University of the Western Cape

URL: http://hdl.handle.net/11394/4340

► Tetrodotoxin (TTX) is a nonpeptidic neurotoxin with a high rate of food poisoning mortality (60%) that has been associated with the consumption of diets from… (more)

▼ Tetrodotoxin (TTX) is a nonpeptidic neurotoxin with a high rate of food poisoning mortality (60%) that has been associated with the consumption of diets from puffer fish and mud snails harbouring TTX-producing bacteria. As this neurotoxin has no known antidote and could not be mitigated by cooking, the only way for safety appears to be the detection of TTX-contaminated fishes at the points of harvest and control. The overall aim of this study was to develop amperometric and impedimetric sensors for TTX based on ionophores and aptamer immobilised on the modified conducting electroactive polyaniline (PANI)/electrode. The undoped polyaniline and poly(4-styrenesulfonic acid) (PSSA) doped electroactive polyanilines were prepared in perchloric acid/acetonitrile and phosphoric acid respectively by electrochemical oxidative polymerisation. Two types of electropolymerisation were applied to prepare the neutral and p-doped PANI−PSSA films composites. The dynamic electroinactivity of TTX was studied which revealed that TTX is not electrochemically active on bare Au, GC, Pt, PG, Ni, Ti and BDD (Boron dopeddiamond) electrodes in acetate buffer pH 4.8. Using ion transfer voltammetry and UV-Vis analysis, the complexation of TTX with two neutral ionophores (sodium ionophore X (NaX) and dibenzo-18-crown6 (B18C6)) was investigated. The cyclic voltammograms (CVs) recorded from ion transfer voltammetry presented no redox peak and no increasing/decreasing current was observed which indicates that no TTX ions transfer from the liquid to the organic phase. In addition, the absorption spectra of the mixture of TTX/NaX and TTX/B18C6 presented the same absorption bands recorded for NaX and B18C6 respectively. Three absorptions bands at 250.4, 278.3, and 370.6 nm for NaX and two at 222.03 and 274.10 nm for B18C6 were observed before and after mixing TTX with NaX and TTX with B18C6 separately. No chemical reaction occurred between the TTX and both ionophores, therefore, sodium ionophore X and dibenzo-18-crown-6 did not form a complex with TTX. Thus, TTX ion sensor cannot be developed based on these two neutral compounds. The electrodynamics of the PANI and PANI−PSSA films electropolymerised on the bare precious metal electrodes were also investigated through various electrochemical techniques. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) studies in sodium phosphate (SPB) and acetate (OAc) buffer revealed that both neutral and p-doped films synthesized were thin (thickness L < 5 nm in acetate buffer and L < 10 nm in sodium phosphate buffer) film polymers. Advisors/Committee Members: Iwuoha, Emmanuel (advisor), Waryo, Tesfaye (advisor).

Subjects/Keywords: Aptasensor; Tetrodotoxin-aptamer; Tetrodotoxin; Ion transfer voltammetry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fomo, G. (2014). Ionophoric and aptameric recognition-modulated electroactive polyaniline films for the determination of tetrodotoxin . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fomo, Gertrude. “Ionophoric and aptameric recognition-modulated electroactive polyaniline films for the determination of tetrodotoxin .” 2014. Thesis, University of the Western Cape. Accessed April 10, 2021. http://hdl.handle.net/11394/4340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fomo, Gertrude. “Ionophoric and aptameric recognition-modulated electroactive polyaniline films for the determination of tetrodotoxin .” 2014. Web. 10 Apr 2021.

Vancouver:

Fomo G. Ionophoric and aptameric recognition-modulated electroactive polyaniline films for the determination of tetrodotoxin . [Internet] [Thesis]. University of the Western Cape; 2014. [cited 2021 Apr 10]. Available from: http://hdl.handle.net/11394/4340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fomo G. Ionophoric and aptameric recognition-modulated electroactive polyaniline films for the determination of tetrodotoxin . [Thesis]. University of the Western Cape; 2014. Available from: http://hdl.handle.net/11394/4340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations