subject:(Apoferritin)

Brno University of Technology

1. Krausová, Kateřina. Studium exprese transferinových receptorů a využití v nanomedicíně: Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/68141

► Bachelor thesis deals with the expression of the transferrin receptor (TfR1) and its use in nanomedicine. During the last decade, nanotechnology emerged as one of… (more)

▼ Bachelor thesis deals with the expression of the transferrin receptor (TfR1) and its use in nanomedicine. During the last decade, nanotechnology emerged as one of the central milestones in connecting all scientific and technological disciplines. Nanomedicine already demonstrated efficacy not only in animal models of cancer but also in clinical practice. The theoretical part is not only aimed at cancer of the human population, but also at the possibilities of targeted drug delivery into the tumor tissue, which greatly reduces the otherwise serious side effects of conventional treatment â€“ systemic toxicity. The practical part is focused on optimization for studying the expression of the transferrin receptor, a protein overexpressed by neoplastic cells aiming to enrich the higher metabolic needs of tumor cells. The optimal conditions were as follows: lysate of 50 000 cells applied with nonreducing nondenaturing buffer and the concentration of the primary antibody of 1.0 đťś‡g/ml. Different levels of TfR1 expression were detected, depending on the type of tumor cells. The cell lines of neuroblastoma, prostate cancer (occurence in every 7th man) and breast cancer (occurence in every 8th woman) were selected for the next experiments. Via this transferrin receptor, apoferritin, which is a protein storaging iron ions in many organisms, can be internalized into cells. Artificially, the internal cavity of apoferritin may be used for encapsulation and transport of any molecules. In the case of this bachelor thesis, the apoferritin was used for delivery of doxorubicin. Doxorubicin has been used for cancer treatment for more than 30 years; however, its administered dose is limited by its high toxicity. This can be reduced by its encapsulation in a suitable vector for targeted transport to the tumor cells only. Apoferritin could serve as such suitable vector. In this thesis, the suitable usage of apoferritin as a nanocarrier for chemotherapeutic delivery was confirmed. Its molecule size of 10-12 nm allows it to employ the effect of increased permeability and retention. At the same time, this size makes it possible to avoid renal clearance. The properties of encapsulated doxorubicin are not affected by apoferritin, thus preserving its toxicity for cells with a high level of TfR1 expression (30% growth inhibition of these cells after 24 h of treatment). Advisors/Committee Members: Heger,, Zbyněk (advisor), Fohlerová, Zdenka (referee).

Subjects/Keywords: Nádorová onemocnění; nanomedicína; apoferritin; doxorubicin; Cancer; nanomedicine; apoferritin; doxorubicin

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APA (6^th Edition):

Krausová, K. (2019). Studium exprese transferinových receptorů a využití v nanomedicíně: Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/68141

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krausová, Kateřina. “Studium exprese transferinových receptorů a využití v nanomedicíně: Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine.” 2019. Thesis, Brno University of Technology. Accessed January 27, 2021. http://hdl.handle.net/11012/68141.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krausová, Kateřina. “Studium exprese transferinových receptorů a využití v nanomedicíně: Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine.” 2019. Web. 27 Jan 2021.

Vancouver:

Krausová K. Studium exprese transferinových receptorů a využití v nanomedicíně: Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/11012/68141.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krausová K. Studium exprese transferinových receptorů a využití v nanomedicíně: Study of expression of transferrin receptors (TfR1) and their utilization in nanomedicine. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/68141

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Arizona State University

2. Clavijo Jordan, Maria Veronica. Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition.

Degree: PhD, Bioengineering, 2012, Arizona State University

URL: http://repository.asu.edu/items/15999

► Sensitivity is a fundamental challenge for in vivo molecular magnetic resonance imaging (MRI). Here, I improve the sensitivity of metal nanoparticle contrast agents by strategically… (more)

Subjects/Keywords: Biomedical engineering; Biophysics; Apoferritin; Manganese; MRI; Tungsten

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clavijo Jordan, M. V. (2012). Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition. (Doctoral Dissertation). Arizona State University. Retrieved from http://repository.asu.edu/items/15999

Chicago Manual of Style (16^th Edition):

Clavijo Jordan, Maria Veronica. “Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition.” 2012. Doctoral Dissertation, Arizona State University. Accessed January 27, 2021. http://repository.asu.edu/items/15999.

MLA Handbook (7^th Edition):

Clavijo Jordan, Maria Veronica. “Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition.” 2012. Web. 27 Jan 2021.

Vancouver:

Clavijo Jordan MV. Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition. [Internet] [Doctoral dissertation]. Arizona State University; 2012. [cited 2021 Jan 27]. Available from: http://repository.asu.edu/items/15999.

Council of Science Editors:

Clavijo Jordan MV. Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition. [Doctoral Dissertation]. Arizona State University; 2012. Available from: http://repository.asu.edu/items/15999

Brno University of Technology

3. Mokrý, Michal. Biokompatibilní proteinové klece pro enkapsulaci a internalizaci malých interferujících RNA: Biocompatible protein cages for encapsulation and internatization of small interfering RNA.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/173605

► This thesis is focused on creation of apoferritin nanocarrier with encapsulated small interfering RNA marked with fluorescent dye. Main objectives are optimization of pH and… (more)

Subjects/Keywords: Nanočastica; nanotransportér apoferitín; siRNA; RNA interferencia; multi-lieková rezistencia; nádorové ochorenie; enkapsulácia.; Nanoparticle; nanocarrier; apoferritin; siRNA; RNA interference; multi drug resistance; cancer; encapsulation.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mokrý, M. (2019). Biokompatibilní proteinové klece pro enkapsulaci a internalizaci malých interferujících RNA: Biocompatible protein cages for encapsulation and internatization of small interfering RNA. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/173605

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mokrý, Michal. “Biokompatibilní proteinové klece pro enkapsulaci a internalizaci malých interferujících RNA: Biocompatible protein cages for encapsulation and internatization of small interfering RNA.” 2019. Thesis, Brno University of Technology. Accessed January 27, 2021. http://hdl.handle.net/11012/173605.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mokrý, Michal. “Biokompatibilní proteinové klece pro enkapsulaci a internalizaci malých interferujících RNA: Biocompatible protein cages for encapsulation and internatization of small interfering RNA.” 2019. Web. 27 Jan 2021.

Vancouver:

Mokrý M. Biokompatibilní proteinové klece pro enkapsulaci a internalizaci malých interferujících RNA: Biocompatible protein cages for encapsulation and internatization of small interfering RNA. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/11012/173605.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mokrý M. Biokompatibilní proteinové klece pro enkapsulaci a internalizaci malých interferujících RNA: Biocompatible protein cages for encapsulation and internatization of small interfering RNA. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/173605

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

4. Bartling, Karsten. Apoferritin Crystallization in relation to Eye Cataract.

Degree: PhD, Bioengineering, 2006, Georgia Tech

URL: http://hdl.handle.net/1853/14111

► Protein crystallization is significant in both biotechnology and biomedical applications. In biotechnology, crystallization is essential for determining the structure of both native and synthesized therapeutically… (more)

▼ Protein crystallization is significant in both biotechnology and biomedical applications. In biotechnology, crystallization is essential for determining the structure of both native and synthesized therapeutically important proteins. It can also be used as a final purification step and as a stable form for protein storage. With regard to biomedical systems, protein crystallization appears to be involved in the development and manifestation of certain human diseases. In particular, there exists evidence that L-rich ferritin crystals are involved in Hereditary Hyperferritinemia Cataract Syndrome (HHCS). In the current research a microbatch crystallization apparatus has been introduced that enables (1) multiple batch crystallization experiments at various temperatures and solution conditions in parallel and (2) quantitative monitoring of crystal growth without disturbing the progress of an experiment for observation. The primary application of the apparatus is, but not limited to, screening of protein crystallization conditions, although the system can also be used for other macromolecular and small-molecule crystallization experiments. Multiwell microbatch experiments demonstrated the dependence of apoferritin crystal growth kinetics and final crystal size on temperature and cadmium concentration. Although the solubility of apoferritin might be independent of temperature, the results of this study show that the crystal growth kinetics are affected by temperature, profoundly under some conditions. For apoferritin under near physiological conditions the solution thermodynamics in the form of the second virial coefficient have proofed to be a valuable predictor for the crystallization outcome. Furthermore, the significance of the elevated level of some divalent cations in cataractous lenses has been studied both in dilute solutions and under crystallization conditions and cadmium seems to be sole menace in apoferritin condensation. Advisors/Committee Members: Ronald W. Rousseau (Committee Chair), Athanassios Sambanis (Committee Co-Chair), Joe LeDoux (Committee Member), Timothy M. Wick (Committee Member), Victor Breedveld (Committee Member).

Subjects/Keywords: Activation energy for crystallization; Cataract; Apoferritin; Second virial coefficient; Crystallization; Thermal gradient; Light Scattering; Microbatch

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bartling, K. (2006). Apoferritin Crystallization in relation to Eye Cataract. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/14111

Chicago Manual of Style (16^th Edition):

Bartling, Karsten. “Apoferritin Crystallization in relation to Eye Cataract.” 2006. Doctoral Dissertation, Georgia Tech. Accessed January 27, 2021. http://hdl.handle.net/1853/14111.

MLA Handbook (7^th Edition):

Bartling, Karsten. “Apoferritin Crystallization in relation to Eye Cataract.” 2006. Web. 27 Jan 2021.

Vancouver:

Bartling K. Apoferritin Crystallization in relation to Eye Cataract. [Internet] [Doctoral dissertation]. Georgia Tech; 2006. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/1853/14111.

Council of Science Editors:

Bartling K. Apoferritin Crystallization in relation to Eye Cataract. [Doctoral Dissertation]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/14111

5. Nwanosike, Quinta M. Effect of divalent cations and solubilizers in apoferritin and gamma D-crystallin solutions: nucleation, crystallization and light scattering studies.

Degree: PhD, Chemical Engineering, 2009, Georgia Tech

URL: http://hdl.handle.net/1853/31736

► Crystallization of proteins in the human body can lead to the development of diseases such as sickle cell anemia and cataract. Understanding protein crystallization can… (more)

▼ Crystallization of proteins in the human body can lead to the development of diseases such as sickle cell anemia and cataract. Understanding protein crystallization can give insight into such diseases. Furthermore, protein crystallization is necessary for protein structure resolution. This is important since resolution of protein structure is the first step towards establishing structure/function relations, and possibly towards performing specific structural modifications that may change the function in desirable directions. Another important application of protein crystallization is in downstream processing in the pharmaceutical industry where it is used for separation and as a final purification step. The present study increases knowledge of interactions between protein molecules during crystallization and hence the crystallization process. Crystallization of proteins in the human body can lead to the development of diseases such as sickle cell anemia and cataract. Understanding the processes involved in protein crystallization can help us gain a better understanding of such diseases. Crystallization of human gamma D-crystallin (HGD) and apoferritin, two proteins found in the lens, was studied in relation to cataract formation. Crystallization of both proteins was studied in the presence of divalent cations which are found at elevated concentrations in cataractous lenses. Results indicate that the divalent cations studied enhance crystallization of these proteins. A thermodynamic property, the osmotic second virial coefficient, was measured in protein solutions and its value was correlated with the occurrence of crystallization. It was found that the second virial coefficient successfully predicted crystallization of both proteins. A new method was developed for indirect measurement of the second virial coefficient using dynamic light scattering. This new method is more robust and efficient than the traditional static light scattering method. Finally the ability of solubilizers to prevent crystallization in HGD solutions was studied. A commercial solubilizer, NDSB-201, was found to increase the energy barrier to nucleation. Although this did not prevent crystallization, it resulted in fewer and smaller crystals being obtained. The naturally occurring alpha A-crystallin was a superior solubilizer to NDSB-201, as it suppressed aggregation and prevented crystallization of HGD under conditions for which NDSB-201 did not. The findings in the present study provide insight into the processes by which protein crystallization occurs and hence into diseases associated with protein crystallization. The findings in the present study provide insight into the processes by which protein crystallization occurs. Using the second virial coefficient to assess whether a protein will crystallize out of solution, approaches for retardation and prevention of protein crystallization, and implications for future research, are discussed. Advisors/Committee Members: Ronald Rousseau (Committee Chair), Athanassios Sambanis (Committee Co-Chair), Amyn Teja (Committee Member), Athanasios Nenes (Committee Member), Ingeborg Schmidt-Krey (Committee Member).

Subjects/Keywords: Light scattering; Cataract; Second virial coefficient; Apoferritin; Protein crystallization; Gamma D-crystallin; Light Scattering; Proteins; Cations; Crystal growth

…D: STATIC LIGHT SCATTERING DATA OBTAINED FOR APOFERRITIN IN CdCl2 SOLUTIONS… …phase diagram of apoferritin in the presence of CdCl2… …140 Table 6-2: Summary of nucleation factors in apoferritin and HGD solutions. The fractal… …apoferritin concentration. The molecular weight of apoferritin was obtained from the intercept… …diffusivity D/Do vs. apoferritin concentration at different concentrations of CdCl2…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nwanosike, Q. M. (2009). Effect of divalent cations and solubilizers in apoferritin and gamma D-crystallin solutions: nucleation, crystallization and light scattering studies. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/31736

Chicago Manual of Style (16^th Edition):

Nwanosike, Quinta M. “Effect of divalent cations and solubilizers in apoferritin and gamma D-crystallin solutions: nucleation, crystallization and light scattering studies.” 2009. Doctoral Dissertation, Georgia Tech. Accessed January 27, 2021. http://hdl.handle.net/1853/31736.

MLA Handbook (7^th Edition):

Nwanosike, Quinta M. “Effect of divalent cations and solubilizers in apoferritin and gamma D-crystallin solutions: nucleation, crystallization and light scattering studies.” 2009. Web. 27 Jan 2021.

Vancouver:

Nwanosike QM. Effect of divalent cations and solubilizers in apoferritin and gamma D-crystallin solutions: nucleation, crystallization and light scattering studies. [Internet] [Doctoral dissertation]. Georgia Tech; 2009. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/1853/31736.

Council of Science Editors:

Nwanosike QM. Effect of divalent cations and solubilizers in apoferritin and gamma D-crystallin solutions: nucleation, crystallization and light scattering studies. [Doctoral Dissertation]. Georgia Tech; 2009. Available from: http://hdl.handle.net/1853/31736

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