subject:(Apical Membrane Antigen 1)

University of Illinois – Urbana-Champaign

1. Milan Noris, Evelia Maria. Development of a plant-based vaccine against malaria.

Degree: PhD, Nutritional Sciences, 2015, University of Illinois – Urbana-Champaign

► Malaria is the most prevalent tropical human disease reported worldwide, caused by protozoan parasites. Half of the world's population is at risk of malaria, and… (more)

▼ Malaria is the most prevalent tropical human disease reported worldwide, caused by protozoan parasites. Half of the world's population is at risk of malaria, and more than 200 million new cases are reported annually. Currently, there are no licensed vaccines available for use. Therefore, there is a vital need for developing an effective and reliable anti-malaria vaccine ideally protecting different parasitic infection stages comprising different antigens that generate appropriate cell-mediated antibody responses of the parasite presentation. Plant-based vaccines serve as novel platforms for developing safe, reliable, and affordable treatments. In this study, a Malchloroplast candidate vaccine is designed, comprised of segments of AMA-1 and MSP-1 proteins along with the GK1 peptide form Teania solium as adjuvant, and expressed in tobacco chloroplasts. Transplastomic tobacco lines have been generated using biolistic transformation, and these are confirmed to carry the synthetic gene construct. The synthetic GK1 peptide is confirmed to be expressed using RT-PCR and Western blots, and detected by RP-HPLC at levels of up to 6 µg g-1 dry weight of tobacco leaf tissue. The plant-derived Malchloroplast candidate vaccine components have been recognized by antibodies in Plasmodium falciparum Malaria patients, and has elicited specific antibodies in subcutaneously immunized BALB/c mice. Additionally, a peptide-based vaccine, Mvac, targeting the MSP1 and AMA1 antigens was evaluated in combination with different adjuvants in an oral and subcutaneous immunization scheme applied to BALB/c mice. Adjuvants tested were plant DNA, pectin, β-subunit of cholera toxin and the GK1 peptide from T. solium. Neither plant DNA nor pectin enhanced the humoral response induced against the Mvac components. While, GK1 peptide had exerted adjuvant effects in terms of the systemic IgG responses induced against the AMA1 peptide, although pectin enhanced the IgA intestinal secretion against both MSP1 and AMA1 antigens. Overall our findings suggest that a multi-component plant-based vaccine against malaria expressing AMA1 and MSP1 antigens, and the GK1 peptide has the potential to serve as a viable and promising low-cost vaccine. As well as oral administration of a vaccine with GK1 peptide has a promising immunogenic effects, proposing that a plant-based vaccine against Malaria administered orally can be effective. Advisors/Committee Members: Juvik, John A (advisor), Pan, Yuan-Xiang (Committee Chair), Korban, Schuyler S (committee member), Rosales Mendoza, Sergio (committee member).

Subjects/Keywords: Malaria; Plant-based vaccine; Plasmodium faciparum; Apical Membrane Antigen 1 (AMA1); Merozoite Surface Protein 1 (MSP1); Plastid transformation; Adjuvant; GK1.

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APA (6^th Edition):

Milan Noris, E. M. (2015). Development of a plant-based vaccine against malaria. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/89223

Chicago Manual of Style (16^th Edition):

Milan Noris, Evelia Maria. “Development of a plant-based vaccine against malaria.” 2015. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 13, 2021. http://hdl.handle.net/2142/89223.

MLA Handbook (7^th Edition):

Milan Noris, Evelia Maria. “Development of a plant-based vaccine against malaria.” 2015. Web. 13 Apr 2021.

Vancouver:

Milan Noris EM. Development of a plant-based vaccine against malaria. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2015. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2142/89223.

Council of Science Editors:

Milan Noris EM. Development of a plant-based vaccine against malaria. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2015. Available from: http://hdl.handle.net/2142/89223

2. Baradji, Issa. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.

Degree: PhD, Veterinary Microbiology, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

► Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected… (more)

▼ Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected to play a role in erythrocyte invasion. To investigate interaction between AMA-1 and the host cell, the ectodomain region of the B. microti ama-1 gene was cloned into an expression vector, expressed as a histidine-tagged fusion protein, and used to probe red blood cell membrane proteins in far Western blot assays. The B. microti ama-1 ectodomain, which excludes the signal peptide and the transmembrane region of the open reading frame, was amplified from a cloned gene sequence. The AMA-1 ectodomain is a membrane bound polypeptide that extends into the extracellular space and is most likely to interact or initiate interaction with the host red blood cell surface receptor(s). The amplicon was ligated into a protein expression vector to produce a 58.1 kDa recombinant His-tagged fusion protein, which was confirmed by Western blot analysis. The recombinant B. microti AMA-1 fusion protein was enriched on nickel affinity columns and then used to probe mouse, human and horse red blood cell membrane proteins in far Western blot assays. Babesia microti AMA-1 consistently reacted strongly with a protein migrating at 49 kDa. A similar reaction occurred between the B. microti AMA-1 and horse red blood cell membrane proteins, suggesting that similar interacting proteins of this size are shared by red blood cells from the three species. The B. microti AMA-1 may bind to red blood cell membrane sialic-acid groups, as shown for other Babesia spp. This may explain the signal at the 49 kDa position observed between B. microti AMA-1 and red blood cell membrane proteins from three different species. Further studies may determine if the binding epitopes of the red blood cell binding partner at this position vary and contribute to the specificity of each parasite AMA-1 for their respective host cells. Advisors/Committee Members: Holman, Patricia J. (advisor), Ball, Judith M. (committee member), Magill, Clint C. (committee member), Womack, James E. (committee member).

Subjects/Keywords: Babesia microti; Apical Membrane Antigen-1; AMA-1; erythrocyte; parasite ligand; red blood cell; receptor; protein-protein interaction

…viii NOMENCLATURE AMA-1 Apical Membrane Antigen –1 BmAMA-1 Babesia microti Apical… …internalization, and multiplication in the host cell. Apical membrane antigen-1 (AMA-1) is one… …Proteins 1 (RAP-1), Babesia bovis Apical membrane Antigen 1 ( BbAMA1), and… …played by parasites ligands Apical Membrane Antigen-1 (AMA-1), Rhoptries Associated… …Membrane Antigen-1 B. microti ama-1 gene Babesia microti ama-1 gene BmAMA-1 protein Babesia…

5 more images…

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APA (6^th Edition):

Baradji, I. (2010). The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

Chicago Manual of Style (16^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

MLA Handbook (7^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Web. 13 Apr 2021.

Vancouver:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

Council of Science Editors:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

University of Queensland

3. Pattinson, David. Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms.

Degree: School of Medicine, 2014, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:347062

Subjects/Keywords: Vaccine; Murine polyomavirus; Virus-like particles; Capsomeres; Bacterial minicells; Malaria; Circumsporozoite protein; Apical membrane antigen-1; Ovalbumin; Cell-mediated immunity; 1107 Immunology

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APA (6^th Edition):

Pattinson, D. (2014). Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:347062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pattinson, David. “Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms.” 2014. Thesis, University of Queensland. Accessed April 13, 2021. http://espace.library.uq.edu.au/view/UQ:347062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pattinson, David. “Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms.” 2014. Web. 13 Apr 2021.

Vancouver:

Pattinson D. Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms. [Internet] [Thesis]. University of Queensland; 2014. [cited 2021 Apr 13]. Available from: http://espace.library.uq.edu.au/view/UQ:347062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pattinson D. Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms. [Thesis]. University of Queensland; 2014. Available from: http://espace.library.uq.edu.au/view/UQ:347062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. フローレス, マリアジョリナルー. Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes : ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究.

Degree: 博士（医学）, 2017, Akita University / 秋田大学

URL: http://hdl.handle.net/10295/2509

► Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because… (more)

Subjects/Keywords: apical membrane; ceruloplasmin; GPI-Cp; hepatocyte

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APA (6^th Edition):

フローレス, �. (2017). Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes : ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究. (Thesis). Akita University / 秋田大学. Retrieved from http://hdl.handle.net/10295/2509

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

フローレス, マリアジョリナルー. “Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes : ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究.” 2017. Thesis, Akita University / 秋田大学. Accessed April 13, 2021. http://hdl.handle.net/10295/2509.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

フローレス, マリアジョリナルー. “Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes : ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究.” 2017. Web. 13 Apr 2021.

Vancouver:

フローレス �. Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes : ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究. [Internet] [Thesis]. Akita University / 秋田大学; 2017. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10295/2509.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

フローレス �. Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes : ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究. [Thesis]. Akita University / 秋田大学; 2017. Available from: http://hdl.handle.net/10295/2509

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. SONG ZHENYING. Amino acid substitutions in the epstein-barr virus associated oncogene latent membrane protein 1 impact upon the immunogenicity of Nasopharyngeal carcinoma cells.

Degree: 2009, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/28156

Subjects/Keywords: Epstein-Barr Virus; latent membrane protein 1; nasopharyngeal carcinoma; antigen presentation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

ZHENYING, S. (2009). Amino acid substitutions in the epstein-barr virus associated oncogene latent membrane protein 1 impact upon the immunogenicity of Nasopharyngeal carcinoma cells. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/28156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ZHENYING, SONG. “Amino acid substitutions in the epstein-barr virus associated oncogene latent membrane protein 1 impact upon the immunogenicity of Nasopharyngeal carcinoma cells.” 2009. Thesis, National University of Singapore. Accessed April 13, 2021. https://scholarbank.nus.edu.sg/handle/10635/28156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ZHENYING, SONG. “Amino acid substitutions in the epstein-barr virus associated oncogene latent membrane protein 1 impact upon the immunogenicity of Nasopharyngeal carcinoma cells.” 2009. Web. 13 Apr 2021.

Vancouver:

ZHENYING S. Amino acid substitutions in the epstein-barr virus associated oncogene latent membrane protein 1 impact upon the immunogenicity of Nasopharyngeal carcinoma cells. [Internet] [Thesis]. National University of Singapore; 2009. [cited 2021 Apr 13]. Available from: https://scholarbank.nus.edu.sg/handle/10635/28156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ZHENYING S. Amino acid substitutions in the epstein-barr virus associated oncogene latent membrane protein 1 impact upon the immunogenicity of Nasopharyngeal carcinoma cells. [Thesis]. National University of Singapore; 2009. Available from: https://scholarbank.nus.edu.sg/handle/10635/28156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Johannes Gutenberg Universität Mainz

6. Fine, Tamir. Mapping the elastic response of epithelial apical cell membranes suspended across porous array.

Degree: 2009, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2010/2174/

► As the elastic response of cell membranes to mechanical stimuli plays a key role in various cellular processes, novel biophysical strategies to quantify the elasticity… (more)

▼ As the elastic response of cell membranes to mechanical stimuli plays a key role in various cellular processes, novel biophysical strategies to quantify the elasticity of native membranes under physiological conditions at a nanometer scale are gaining interest. In order to investigate the elastic response of apical membranes, elasticity maps of native membrane sheets, isolated from MDCK II (Madine Darby Canine kidney strain II) epithelial cells, were recorded by local indentation with an Atomic Force Microscope (AFM). To exclude the underlying substrate effect on membrane indentation, a highly ordered gold coated porous array with a pore diameter of 1.2 μm was used to support apical membranes. Overlays of fluorescence and AFM images show that intact apical membrane sheets are attached to poly-D-lysine coated porous substrate. Force indentation measurements reveal an extremely soft elastic membrane response if it is indented at the center of the pore in comparison to a hard repulsion on the adjacent rim used to define the exact contact point. A linear dependency of force versus indentation (-dF/dh) up to 100 nm penetration depth enabled us to define an apparent membrane spring constant (kapp) as the slope of a linear fit with a stiffness value of for native apical membrane in PBS. A correlation between fluorescence intensity and kapp is also reported. Time dependent hysteresis observed with native membranes is explained by a viscoelastic solid model of a spring connected to a Kelvin-Voight solid with a time constant of 0.04 s. No hysteresis was reported with chemically fixated membranes. A combined linear and non linear elastic response is suggested to relate the experimental data of force indentation curves to the elastic modulus and the membrane thickness. Membrane bending is the dominant contributor to linear elastic indentation at low loads, whereas stretching is the dominant contributor for non linear elastic response at higher loads. The membrane elastic response was controlled either by stiffening with chemical fixatives or by softening with F-actin disrupters. Overall, the presented setup is ideally suitable to study the interactions of the apical membrane with the underlying cytoskeleton by means of force indentation elasticity maps combined with fluorescence imaging.

Subjects/Keywords: AFM, Elasticity, Apical Membrane, Indentation; Natural sciences and mathematics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fine, T. (2009). Mapping the elastic response of epithelial apical cell membranes suspended across porous array. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2010/2174/

Chicago Manual of Style (16^th Edition):

Fine, Tamir. “Mapping the elastic response of epithelial apical cell membranes suspended across porous array.” 2009. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed April 13, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2010/2174/.

MLA Handbook (7^th Edition):

Fine, Tamir. “Mapping the elastic response of epithelial apical cell membranes suspended across porous array.” 2009. Web. 13 Apr 2021.

Vancouver:

Fine T. Mapping the elastic response of epithelial apical cell membranes suspended across porous array. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2009. [cited 2021 Apr 13]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2010/2174/.

Council of Science Editors:

Fine T. Mapping the elastic response of epithelial apical cell membranes suspended across porous array. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2009. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2010/2174/

University of Guelph

7. Lackeyram, Dale, Anthony. Expression of the Small Intestinal Apical Membrane Hydrolases in the Early-Weaned Piglet.

Degree: PhD, Department of Animal and Poultry Science, 2012, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3628

► The small intestinal mucosal apical hydrolases are essential to the terminal digestion of enteral nutrients such as carbohydrates, proteins, fats and phosphates, and non-immune defense.… (more)

▼ The small intestinal mucosal apical hydrolases are essential to the terminal digestion of enteral nutrients such as carbohydrates, proteins, fats and phosphates, and non-immune defense. Weaning results in the complete replacement of fetal enterocytes with mature adult-type enterocytes and is typified by mucosal atrophy, crypt hyperplasia and compromised digestive and defensive functions. Given these severe physiological changes, we hypothesize that the major apical small intestinal hydrolases will be differentially expressed, allowing for reprogramming and adaptation, in the early-weaned piglet. Therefore, the objectives of this study were to examine changes in the digestive capacity, the catalytic kinetics, and abundances of protein and mRNA of the small intestinal hydrolases, including alkaline phosphatase (IAP), lactase phlorizin hydrolase (LPH), sucrase-isomaltase (SI), maltase-glucoamylase (MGA) and aminopeptidase N (APN), in the early-weaned pigs in comparison with suckling pigs. A total of 20 Yorkshire piglets, 10 suckling (SU) and 10 early-weaned (WN) with an average initial body weight of about 3 kg at the age of 10 d, were used in this study. Weanling piglets were fed a corn and soybean meal-based diet for 12 d. Proximal jejunal samples from both groups were collected. Hydrolase kinetic experiments were conducted using the substrates of lactose (0-75 mM), sucrose (0-75 mM), maltose (0-75 mM), amylose (0-100 mM), p-nitrophenyl phosphate (0-10 mM), and L-alanine-p-nitroanilide hydrochloride (0-16 mM). Abundances of the target gene hydrolase protein and mRNA were analyzed by Western blotting and quantitative real time reverse transcription- polymerase chain reaction (RT-PCR), respectively, using ß-actin as a control. Results from this study demonstrate that early weaning down-regulated (P < 0.05) the digestive capacity and expression of LPH while simultaneously increasing (P < 0.05) the digestive capacity and expression of SI and MGA. Furthermore, weaning decreased (P < 0.05) the digestive capacity and expression of APN and IAP by 35 and 50%, respectively. Thus, the early-weaning process differentially affected the expression of the apical membrane-bound hydrolases of the small intestine. The down-regulation of IAP highlights the reduced microbial detoxifying capacity of the newly weaned piglet and provides some insight into the cascade of immune related events that occur during the post-weaning transition period. The reduced expression of LPH and the simultaneous up-regulation of SI, maltase, and MGA indicate the unique nature of the small intestinal reprogramming that occurs during weaning. These results imply that the early weaning events help the small intestine adapt to the transition to starch digestion. Meanwhile, the down-regulation of the APN expression may be partially responsible for the reduced efficiency of whole body protein utilization, and the pervasive localized immune responses observed in the small intestine of early-weaned piglets. Advisors/Committee Members: Fan, Ming, Z (advisor).

Subjects/Keywords: Early Weaning; Small Intestinal Hydrolases; Apical membrane hydrolases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lackeyram, Dale, A. (2012). Expression of the Small Intestinal Apical Membrane Hydrolases in the Early-Weaned Piglet. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3628

Chicago Manual of Style (16^th Edition):

Lackeyram, Dale, Anthony. “Expression of the Small Intestinal Apical Membrane Hydrolases in the Early-Weaned Piglet.” 2012. Doctoral Dissertation, University of Guelph. Accessed April 13, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3628.

MLA Handbook (7^th Edition):

Lackeyram, Dale, Anthony. “Expression of the Small Intestinal Apical Membrane Hydrolases in the Early-Weaned Piglet.” 2012. Web. 13 Apr 2021.

Vancouver:

Lackeyram, Dale A. Expression of the Small Intestinal Apical Membrane Hydrolases in the Early-Weaned Piglet. [Internet] [Doctoral dissertation]. University of Guelph; 2012. [cited 2021 Apr 13]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3628.

Council of Science Editors:

Lackeyram, Dale A. Expression of the Small Intestinal Apical Membrane Hydrolases in the Early-Weaned Piglet. [Doctoral Dissertation]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3628

8. Lentini, Gaëlle. Identification de nouvelles protéines régulées différentiellement au cours du cycle cellulaire de Toxoplasma gondii : Identification of new proteins differentially regulated along the cell cycle of Toxoplasma gondii.

Degree: Docteur es, Biologie Santé, 2015, Montpellier

URL: http://www.theses.fr/2015MONTT049

►

Toxoplasma gondii est un protiste apicomplexe responsable de la toxoplasmose. Ce parasite intracellulaire obligatoire possède des organites sécrétoires apicaux dont les rhoptries qui contiennent des… (more)

▼

Toxoplasma gondii est un protiste apicomplexe responsable de la toxoplasmose. Ce parasite intracellulaire obligatoire possède des organites sécrétoires apicaux dont les rhoptries qui contiennent des facteurs de virulence essentiels à l'invasion et à la modulation de la cellule hôte qu'il infecte. Au cours de la division cellulaire de T. gondii, les protéines de rhoptries sont synthétisées selon la même cinétique. Dans le but d'identifier de nouvelles protéines dont la fonction est potentiellement liée aux rhoptries, nous avons recherché à partir des bases de données du génome de T. gondii, les protéines présentant ce profil particulier d'expression. La localisation subcellulaire de 12 candidats a été réalisée puis une caractérisation phénotypique de quatre d'entre eux a été entreprise. Nous avons identifié une nouvelle protéase de rhoptries, DegP, essentielle à la virulence du parasite in vivo. Nous montrons que DegP contrôle la phase aigüe de l'infection en modulant la réponse immune de l'hôte contribuant ainsi à la dissémination du parasite in vivo. Nous identifions également deux protéines homologues, Claw1 et Claw2, présentant une localisation atypique à l'extrémité apicale du parasite. Notre incapacité à déléter ces gènes pourrait indiquer un rôle essentiel de ces protéines au niveau du complexe apical de T. gondii. Enfin, bien que n'étant pas reliée aux rhoptries, ce crible a permis d'identifier la première protéine associée aux jonctions des vésicules constituant le complexe membranaire interne de Toxoplasma. La délétion de cette protéine, SIP, affecte la forme du parasite, entrainant un défaut de motilité, d'invasion et de virulence in vivo.

Toxoplasma gondii is an apicomplexan protist and the causative agent of toxoplasmosis. This obligate intracellular parasite harbors apical secretory organelles such as rhoptries that contain essential virulence factors responsible of the invasion and the modulation of the infected host cell. Along the cell cycle of T. gondii, rhoptry proteins share the same timing of expression. In order to identify new proteins involve in rhoptry content, biogenesis or secretion, we screened the genome database of T. gondii to isolate proteins that present this particular profile. We obtained the subcellular localization of 12 candidates and we investigated the biological functions for 4 of them. We showed that DegP, a rhoptry protease is essential for the in vivo virulence of T. gondii. DegP controls the acute phase during infection and modulate the host immune response leading to better parasite dissemination in vivo. Also, we identified Claw1 and its paralog Claw2 that present an atypical localization at the apical end of the parasite. To date, we were unable to disrupt the genes encoding these proteins suggesting that they may have an essential function related to the apical complex in T. gondii. Finally, we also examined a ‘hit' of this screening that was not related to rhoptries and we identified SIP, the first protein associated with the transversal junctions of the inner…

Advisors/Committee Members: Lebrun, Maryse (thesis director).

Subjects/Keywords: Toxoplasma gondii; Apicomplexes; Rhoptries; Protease; Complexe membranaire interne; Complexe apical; Toxoplasma gondii; Apicomplexa; Rhoptry; Protease; Inner membrane complex; Apical complex

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lentini, G. (2015). Identification de nouvelles protéines régulées différentiellement au cours du cycle cellulaire de Toxoplasma gondii : Identification of new proteins differentially regulated along the cell cycle of Toxoplasma gondii. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2015MONTT049

Chicago Manual of Style (16^th Edition):

Lentini, Gaëlle. “Identification de nouvelles protéines régulées différentiellement au cours du cycle cellulaire de Toxoplasma gondii : Identification of new proteins differentially regulated along the cell cycle of Toxoplasma gondii.” 2015. Doctoral Dissertation, Montpellier. Accessed April 13, 2021. http://www.theses.fr/2015MONTT049.

MLA Handbook (7^th Edition):

Lentini, Gaëlle. “Identification de nouvelles protéines régulées différentiellement au cours du cycle cellulaire de Toxoplasma gondii : Identification of new proteins differentially regulated along the cell cycle of Toxoplasma gondii.” 2015. Web. 13 Apr 2021.

Vancouver:

Lentini G. Identification de nouvelles protéines régulées différentiellement au cours du cycle cellulaire de Toxoplasma gondii : Identification of new proteins differentially regulated along the cell cycle of Toxoplasma gondii. [Internet] [Doctoral dissertation]. Montpellier; 2015. [cited 2021 Apr 13]. Available from: http://www.theses.fr/2015MONTT049.

Council of Science Editors:

Lentini G. Identification de nouvelles protéines régulées différentiellement au cours du cycle cellulaire de Toxoplasma gondii : Identification of new proteins differentially regulated along the cell cycle of Toxoplasma gondii. [Doctoral Dissertation]. Montpellier; 2015. Available from: http://www.theses.fr/2015MONTT049

9. Tulio Lorenzo Olano Dextre. Susceptibilidade genética a periodontite apical crônica em indivíduos com fissura labiopalatina: estudo caso-controle.

Degree: 2016, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/61/61132/tde-18102016-154607/

► A periodontite apical é um processo inflamatório que ocorre na região do periápice, em decorrência de contaminação microbiana do sistema de canais que se origina… (more)

▼ A periodontite apical é um processo inflamatório que ocorre na região do periápice, em decorrência de contaminação microbiana do sistema de canais que se origina a partir da necrose pulpar ou canais radiculares tratados inadequadamente. Estudos recentes avaliando os fatores genéticos envolvidos no desenvolvimento da periodontite apical crônica (PAC) relatam forte relação com uma predisposição genética do indivíduo e determinam que variantes polimórficas em certos genes envolvidos na remodelação da matriz óssea poderiam contribuir na persistência desta. As metaloproteinases da matriz (MMPs) são enzimas que estão fortemente associadas com os níveis de inflamação e desempenham um papel importante na remodelação e reabsorção óssea, e níveis elevados de MMPs têm relação com a deficiência de reparo da lesão induzindo a PAC. Além disso, as MMPs também participam do complexo desenvolvimento raniofacial o que faz com que também sejam consideradas para a ocorrência de malformações faciais. O objetivo deste trabalho foi determinar se variantes polimórficas nos genes MMP-2 e MMP-3, envolvidos na resposta inflamatória estão associados a permanência de periodontite apical persistente após o tratamento endodôntico em indivíduos com fissura labiopalatina. Foram selecionados para este estudo 180 indivíduos divididos em 3 grupos: GI: 34 indivíduos com fissura labiopalatina, não sindrômicos, com história de PAC relacionada ao tratamento endodôntico; GII: 45 indivíduos sem fissura labiopalatina, não sindrômicos com história de PAC e GIII: grupo controle composto por 101 indivíduos sem fissura e sem relato de PAC. Como critério de inclusão para o diagnóstico de PAC foram considerados os escores 4 e 5 do índice PAI (PeriApical Index) analisado em radiografias periapicais de controle de um ano ou mais após o tratamento dos dentes envolvidos. Foram selecionadas para a genotipagem 3 variantes polimórficas no gene MMP-2 (rs243865, rs2285053 e rs2287074) e 2 no gene MMP-3 (rs679620 e rs522616). Os resultados foram analisados usando o software SDS 1.7 (Applied Biosystems) e os dados foram tabulados no programa Excel 8.0. As comparações entre as frequências dos genótipos e alelos foram realizadas através do teste do qui-quadrado (2) e Odds Ratio com intervalo de confiança de 95% (IC). Valores de p<0,05 foram considerados estatisticamente significativos. Das variantes polimórficas pesquisadas neste grupo populacional brasileiro, foi encontrada associação positiva do rs679620 no gene MMP-3 com a fissura labiopalatina e PAC somente quando comparado com o grupo com PAC e sem fissura. Sendo encontrada associação positiva também do rs522616 no gene MMP-3 com PAC e sem fissura, somente no comparativo com o grupo controle. Não foi encontrada nenhuma associação positiva das variantes no gene MMP-2 (rs243865, rs2285053 e rs2287074) nem com PAC nem com fissura labiopalatina. Dessa forma, são necessários estudos semelhantes analisando outras variantes polimórficas em outros genes envolvidos na cascata de sinalização da inflamação e na embriologia… Advisors/Committee Members: Lucimara Teixeira das Neves, Flaviana Bombarda de Andrade, Celso Kenji Nishiyama, Camila de Oliveira Rodini Pegoraro, Lidiane de Castro Pinto.

Subjects/Keywords: Fenda Labial; Fissura Palatina; Metaloproteinase da Membrana; Periodontite Apical; Apical Periodontitis; Cleft Lip; Cleft Palate; Matrix Metalloproteinases; Membrane-Associated

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dextre, T. L. O. (2016). Susceptibilidade genética a periodontite apical crônica em indivíduos com fissura labiopalatina: estudo caso-controle. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/61/61132/tde-18102016-154607/

Chicago Manual of Style (16^th Edition):

Dextre, Tulio Lorenzo Olano. “Susceptibilidade genética a periodontite apical crônica em indivíduos com fissura labiopalatina: estudo caso-controle.” 2016. Doctoral Dissertation, University of São Paulo. Accessed April 13, 2021. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-18102016-154607/.

MLA Handbook (7^th Edition):

Dextre, Tulio Lorenzo Olano. “Susceptibilidade genética a periodontite apical crônica em indivíduos com fissura labiopalatina: estudo caso-controle.” 2016. Web. 13 Apr 2021.

Vancouver:

Dextre TLO. Susceptibilidade genética a periodontite apical crônica em indivíduos com fissura labiopalatina: estudo caso-controle. [Internet] [Doctoral dissertation]. University of São Paulo; 2016. [cited 2021 Apr 13]. Available from: http://www.teses.usp.br/teses/disponiveis/61/61132/tde-18102016-154607/.

Council of Science Editors:

Dextre TLO. Susceptibilidade genética a periodontite apical crônica em indivíduos com fissura labiopalatina: estudo caso-controle. [Doctoral Dissertation]. University of São Paulo; 2016. Available from: http://www.teses.usp.br/teses/disponiveis/61/61132/tde-18102016-154607/

University of Southern California

10. Sura, Asmiti Vivek. Characterization of the retromer complex of proteins in gastric parietal cells.

Degree: MS, Biochemistry and Molecular Biology, 2013, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/307627/rec/1312

► Gastric acid secretion involves the regulated recycling of the H, K-ATPase to and from the apical membrane of parietal cells. All of the steps in… (more)

Subjects/Keywords: apical membrane recycling; gastric microsomes; H K-ATPase; hydrogen-potassium-adenosinetriphosphatase; retromer; tubulovesicles

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APA (6^th Edition):

Sura, A. V. (2013). Characterization of the retromer complex of proteins in gastric parietal cells. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/307627/rec/1312

Chicago Manual of Style (16^th Edition):

Sura, Asmiti Vivek. “Characterization of the retromer complex of proteins in gastric parietal cells.” 2013. Masters Thesis, University of Southern California. Accessed April 13, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/307627/rec/1312.

MLA Handbook (7^th Edition):

Sura, Asmiti Vivek. “Characterization of the retromer complex of proteins in gastric parietal cells.” 2013. Web. 13 Apr 2021.

Vancouver:

Sura AV. Characterization of the retromer complex of proteins in gastric parietal cells. [Internet] [Masters thesis]. University of Southern California; 2013. [cited 2021 Apr 13]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/307627/rec/1312.

Council of Science Editors:

Sura AV. Characterization of the retromer complex of proteins in gastric parietal cells. [Masters Thesis]. University of Southern California; 2013. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/307627/rec/1312

11. Brar, Harmenjit Singh. Triple Positive Microparticles as a “Liquid Biopsy” for Risk Stratification of Prostate Cancer.

Degree: 2017, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/4933

► Prostate cancer remains a substantial contributor to cancer-related mortality worldwide. Current screening methods include obtaining a PSA blood test. However, controversy surrounds its use as… (more)

Subjects/Keywords: Prostate Cancer; Extracellular Vesicles; Prostate Specific Membrane Antigen; Six-transmembrane Epithelial Antigen of the Prostate-1; GHSR1a; CD151; Surgery; Urology

…Doubling Time PSMA Prostate Specific Membrane Antigen ROC Receiver Operating Curve ROI… …transmembrane Epithelial Antigen of the Prostate-1 TCGA The Cancer Genome Atlas TMA Tissue… …using metastatic prostate cancer patient plasma. For Prostate Specific Membrane Antigen clone… …study (A). For Six Transmembrane Antigen of Prostate-1 conjugated with Alexa Fluor… …secretagogue receptor HG PIN High Grade Prostatic Intraepithelial Neoplasia IGF-1 Insulin-like…

10 more images…

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APA (6^th Edition):

Brar, H. S. (2017). Triple Positive Microparticles as a “Liquid Biopsy” for Risk Stratification of Prostate Cancer. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/4933

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Brar, Harmenjit Singh. “Triple Positive Microparticles as a “Liquid Biopsy” for Risk Stratification of Prostate Cancer.” 2017. Thesis, University of Western Ontario. Accessed April 13, 2021. https://ir.lib.uwo.ca/etd/4933.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Brar, Harmenjit Singh. “Triple Positive Microparticles as a “Liquid Biopsy” for Risk Stratification of Prostate Cancer.” 2017. Web. 13 Apr 2021.

Vancouver:

Brar HS. Triple Positive Microparticles as a “Liquid Biopsy” for Risk Stratification of Prostate Cancer. [Internet] [Thesis]. University of Western Ontario; 2017. [cited 2021 Apr 13]. Available from: https://ir.lib.uwo.ca/etd/4933.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Brar HS. Triple Positive Microparticles as a “Liquid Biopsy” for Risk Stratification of Prostate Cancer. [Thesis]. University of Western Ontario; 2017. Available from: https://ir.lib.uwo.ca/etd/4933

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

12. Brown, Joel. INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS.

Degree: PhD, Genetics and Development, 2018, Cornell University

URL: http://hdl.handle.net/1813/59291

► Forward genetics allows the identification of novel genes involved in a particular biological process. We performed an ENU forward mutagenesis screen in mice aimed at… (more)

▼ Forward genetics allows the identification of novel genes involved in a particular biological process. We performed an ENU forward mutagenesis screen in mice aimed at identifying genes which regulate early organ morphogenesis during development. Fifteen mouse mutants were identified in the screen which possess cardiovascular, craniofacial, extraembryonic, or general body morphology defects. After performing preliminary characterization of all 15 lines, we identified a likely causative mutation in 14 of the 15 mutants. 1D and 13B mutants both exhibit craniofacial defects and were selected for in-depth characterization to identify the molecular mechanisms whereby they regulate embryogenesis. 1D mutants are characterized by an open neural tube in the hindbrain region. This phenotype is similar to human embryos with exencephaly, a congenital birth malformation of high incidence in the human population. Positional cloning of 1D identified a mutation in SPCA1, a Golgi-localized pump that controls calcium homeostasis. Results from the molecular characterization of mouse 1D mutants, as well as from time lapse microscopy of chicken embryos, revealed that calcium is tightly regulated during neural tube closure and that calcium homeostasis is required to promote apical constriction of neuroepithelial cells. These results show that SPCA1 activity is required to regulate the actomyosin dynamics that propel apical constriction, and that the actin severing protein, Cofilin 1, is a key mediator of SPCA1 function. Together, my findings provide the first genetic evidence that calcium homeostasis is needed for neural tube closure, opening a new window into understanding the etiology of human neural tube defects. 13B mutants have neural tube defects accompanied by a suite of other malformations including randomized L-R patterning and maxillary overgrowth. Molecular characterization revealed that all 13B phenotypes result from the absence of cilia, an organelle important for neural tube patterning and for the establishment of L-R asymmetry. Exome sequencing of 13B embryos identified a nonsense mutation in Pibf1. I show that PIBF1 is required for ciliogenesis during early embryonic development and identify a novel role for PIBF1 in regulating centrosome duplication. These findings highlight the importance of PIBF1 in regulating multiple aspects of centrosome biology, and provide a model for understanding how defects in these processes contribute to human ciliopathies. Advisors/Committee Members: Garcia-Garcia, Maria J. (chair), Schimenti, John C. (committee member), Kurpios, Natasza (committee member).

Subjects/Keywords: calcium; Actomyosin; Apical Constriction; Cofilin 1; Neural Tube; SPCA1; Developmental biology; Genetics; Molecular biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brown, J. (2018). INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/59291

Chicago Manual of Style (16^th Edition):

Brown, Joel. “INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS.” 2018. Doctoral Dissertation, Cornell University. Accessed April 13, 2021. http://hdl.handle.net/1813/59291.

MLA Handbook (7^th Edition):

Brown, Joel. “INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS.” 2018. Web. 13 Apr 2021.

Vancouver:

Brown J. INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS. [Internet] [Doctoral dissertation]. Cornell University; 2018. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1813/59291.

Council of Science Editors:

Brown J. INVESTIGATING MAMMALIAN DEVELOPMENT USING MOUSE FORWARD GENETICS. [Doctoral Dissertation]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59291

NSYSU

13. Kao, Pei-Hsiu. Molecular mechanism of membrane components on modulating membrane-damaging activity of Naja naja atra cardiotoxins.

Degree: PhD, Institute of Biomedical Sciences, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706112-155756

► Naja naja atra Cardiotoxins (CTXs), basic polypeptides of 60 amino acid residues adopt a three-fingered loop-folding topology and show cytotoxicity for human tissues in targeting… (more)

▼ Naja naja atra Cardiotoxins (CTXs), basic polypeptides of 60 amino acid residues adopt a three-fingered loop-folding topology and show cytotoxicity for human tissues in targeting cell membrane. Despite having highly similar sequence, the six CTX isoforms also display different cytotoxic potencies and hemolytic activities. The goal of these studies is to explore the mechanical processes that involved in membrane-damaging activities of CTXs on vesicles composed of different cell membrane components, and to delineate the events that lead to different biological activities of CTXs. The studies were performed by estimating the color transformation of phospholipid/polydiacetylene vesicles and the fluorescence enhancement of fluorescein-labeled phospholipid/protein or fluorescein released from vesicles. It was found that vesicles consisted of unsaturated phospholipids improve membrane-damaging activity of CTXs and adopt a vital membrane-bound conformation of CTXs. In contract, the characteristic of vesicles consisted of saturated phospholipids was against CTXs adopting an essential membrane-damaging structure. It was also found that not only electrostatic force but also hydrophobic force were involved in the interaction between CTXs and membrane. Comparing with phosphatidylcholine-only vesicles, CTXs displayed higher membrane-damaging activity for the sphingomyelin-containing vesicles, and the loop2 region of CTXs play a crucial role for the membrane-damaging activity of sphingomyelin-containing vesicles. Besides, the CTX3 and CTX5 would interact with the H-antigen of blood group O red blood cells, but only the binding of CTX3 with H-antigen reduce its membrane-damaging activity for red blood cells membrane. Moreover, the fusogenicity of CTXs is responsible for the membrane-damaging activity of CTXs toward bacterial membrane-mimicking vesicles. The cardiolipin have the potency to improve the fusogenicity of CTX3, which induced the bactericidal activity toward the cardiolipin-containing bacterium. Advisors/Committee Members: Chien-Cheng Chen (chair), Kuang-hung Cheng (chair), Shinne-Ren Lin (chair), Chun-Chang Chang (chair), Long-Sen Chang (committee member).

Subjects/Keywords: phospholipid vesicle; H-antigen; cardiolipin; cardiotoxin; membrane-damaging activity; sphingomyelin

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APA (6^th Edition):

Kao, P. (2012). Molecular mechanism of membrane components on modulating membrane-damaging activity of Naja naja atra cardiotoxins. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706112-155756

Chicago Manual of Style (16^th Edition):

Kao, Pei-Hsiu. “Molecular mechanism of membrane components on modulating membrane-damaging activity of Naja naja atra cardiotoxins.” 2012. Doctoral Dissertation, NSYSU. Accessed April 13, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706112-155756.

MLA Handbook (7^th Edition):

Kao, Pei-Hsiu. “Molecular mechanism of membrane components on modulating membrane-damaging activity of Naja naja atra cardiotoxins.” 2012. Web. 13 Apr 2021.

Vancouver:

Kao P. Molecular mechanism of membrane components on modulating membrane-damaging activity of Naja naja atra cardiotoxins. [Internet] [Doctoral dissertation]. NSYSU; 2012. [cited 2021 Apr 13]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706112-155756.

Council of Science Editors:

Kao P. Molecular mechanism of membrane components on modulating membrane-damaging activity of Naja naja atra cardiotoxins. [Doctoral Dissertation]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706112-155756

University of Maryland

14. Miller, Heather. The regulation of B cell activation by membrane damage and antigen density.

Degree: Cell Biology & Molecular Genetics, 2015, University of Maryland

URL: http://hdl.handle.net/1903/16960

► Antibodies generated by B cells neutralize pathogens and pathogen-secreted toxins and flag them for immune clearance. Antibody responses are initiated via binding of cognate antigen… (more)

▼ Antibodies generated by B cells neutralize pathogens and pathogen-secreted toxins and flag them for immune clearance. Antibody responses are initiated via binding of cognate antigen to B-cell receptors (BCR). This induces BCR aggregation in lipid rafts, promoting BCR signaling and internalization of antigen for processing and presentation to T helper cells, which is essential for generating high affinity and long-lasting antibody responses. The ability of an immunogen to activate BCR signaling and internalization is necessary for efficient vaccines. To capture these immunogens, B cells circulate and migrate through blood and lymphoid tissues. During circulation, the plasma membrane of B cells may be damaged by mechanical forces and membrane-perforating toxins. The impact of plasma membrane damage on B-cell activation is unknown. The first part of this thesis investigated the mechanism of plasma membrane repair in B cells and the effects of repair on BCR activation. My research reveals that B cells rapidly repair membrane wounds provoked by streptolysin O, a pore forming bacterial toxin. Similar to the mechanism reported for fibroblasts and muscle cells, B cells repair by Ca2+ triggered lysosome exocytosis, which releases acid sphingomyelinase (ASM) to the plasma membrane to induce endocytosis of damaged membrane. Different from previous reports, ASM induces direct endocytosis of lipid rafts in the absence of the membrane invaginating lipid raft protein, caveolin. Importantly, it was discovered that BCR activation interferes with plasma membrane repair, while wounding inhibits BCR signaling and internalization by segregating BCRs from lipid rafts. These data suggest that plasma membrane repair and B cell activation interfere with one another due to competition for lipid rafts. The second part of my thesis established and characterized a membrane-bound antigen system where all antigenic molecules are optimally oriented for BCR binding. Using the new system, we investigated the role of the density and valency of membrane-bound antigen on BCR activation. The results show that increases in the density but not valency of antigen on membranes significantly enhance the magnitudes of the early events of BCR activation, including BCR self-clustering, cell spreading on antigen-presenting surface, and protein tyrosine phosphorylation. The enhanced signaling is correlated with greater actin dynamics required for BCR aggregation, B-cell spreading and signaling. These results indicate that this model antigen will benefit quantitative studies of the molecular mechanisms underlying BCR activation, and also suggest that manipulations of molecular configuration and density can be applied to enhance the immunogenicity of vaccines. Advisors/Committee Members: Song, Wenxia (advisor).

Subjects/Keywords: Immunology; Cellular biology; antigen density; B cell; membrane repair

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miller, H. (2015). The regulation of B cell activation by membrane damage and antigen density. (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/16960

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Miller, Heather. “The regulation of B cell activation by membrane damage and antigen density.” 2015. Thesis, University of Maryland. Accessed April 13, 2021. http://hdl.handle.net/1903/16960.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Miller, Heather. “The regulation of B cell activation by membrane damage and antigen density.” 2015. Web. 13 Apr 2021.

Vancouver:

Miller H. The regulation of B cell activation by membrane damage and antigen density. [Internet] [Thesis]. University of Maryland; 2015. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1903/16960.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Miller H. The regulation of B cell activation by membrane damage and antigen density. [Thesis]. University of Maryland; 2015. Available from: http://hdl.handle.net/1903/16960

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

15. Jin, Dongbin. Stimulation of CD8 T cells with membrane vesicles prepared from antigen presenting cells.

Degree: Clinical School - St Vincent's Hospital, 2015, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/54605 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35345/SOURCE02?view=true

► Immunotherapy has emerged as a promising tool to treat diseases via enhancing or suppressing immune responses of T cells. For infectious diseases and cancer, inducing… (more)

Subjects/Keywords: Membrane vesicle; Immunotherapy; CD8 T cell; Antigen presenting cell; Dendritic cell

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APA (6^th Edition):

Jin, D. (2015). Stimulation of CD8 T cells with membrane vesicles prepared from antigen presenting cells. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54605 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35345/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Jin, Dongbin. “Stimulation of CD8 T cells with membrane vesicles prepared from antigen presenting cells.” 2015. Doctoral Dissertation, University of New South Wales. Accessed April 13, 2021. http://handle.unsw.edu.au/1959.4/54605 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35345/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Jin, Dongbin. “Stimulation of CD8 T cells with membrane vesicles prepared from antigen presenting cells.” 2015. Web. 13 Apr 2021.

Vancouver:

Jin D. Stimulation of CD8 T cells with membrane vesicles prepared from antigen presenting cells. [Internet] [Doctoral dissertation]. University of New South Wales; 2015. [cited 2021 Apr 13]. Available from: http://handle.unsw.edu.au/1959.4/54605 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35345/SOURCE02?view=true.

Council of Science Editors:

Jin D. Stimulation of CD8 T cells with membrane vesicles prepared from antigen presenting cells. [Doctoral Dissertation]. University of New South Wales; 2015. Available from: http://handle.unsw.edu.au/1959.4/54605 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35345/SOURCE02?view=true

University of Pennsylvania

16. Santoro, Stephen. T Cells Bearing a Chimeric Antigen Receptor Against the Tumor Vasculature Destroy the Tumor Endothelium and Result in Tumor Regression.

Degree: 2014, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1432

► Aberrant blood vessels enable tumor growth, provide a barrier to immune infiltration, and serve as a source of pro-tumorigenic signals. Targeting tumor blood vessels for… (more)

Subjects/Keywords: Adoptive therapy; Chimeric antigen receptor; Endothelial cells; Prostate-specific membrane antigen; Vascular disruption; Biology; Cell Biology; Oncology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santoro, S. (2014). T Cells Bearing a Chimeric Antigen Receptor Against the Tumor Vasculature Destroy the Tumor Endothelium and Result in Tumor Regression. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1432

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santoro, Stephen. “T Cells Bearing a Chimeric Antigen Receptor Against the Tumor Vasculature Destroy the Tumor Endothelium and Result in Tumor Regression.” 2014. Thesis, University of Pennsylvania. Accessed April 13, 2021. https://repository.upenn.edu/edissertations/1432.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santoro, Stephen. “T Cells Bearing a Chimeric Antigen Receptor Against the Tumor Vasculature Destroy the Tumor Endothelium and Result in Tumor Regression.” 2014. Web. 13 Apr 2021.

Vancouver:

Santoro S. T Cells Bearing a Chimeric Antigen Receptor Against the Tumor Vasculature Destroy the Tumor Endothelium and Result in Tumor Regression. [Internet] [Thesis]. University of Pennsylvania; 2014. [cited 2021 Apr 13]. Available from: https://repository.upenn.edu/edissertations/1432.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santoro S. T Cells Bearing a Chimeric Antigen Receptor Against the Tumor Vasculature Destroy the Tumor Endothelium and Result in Tumor Regression. [Thesis]. University of Pennsylvania; 2014. Available from: https://repository.upenn.edu/edissertations/1432

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

17. Suresh, Shruthy. The Integrated Stress Response Pathway Regulates PD-L1 Translation in Human Lung Cancer.

Degree: 2019, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/6624

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The student had requested that her name appear as "Shruthy Suresh" on the ETD record even though her name appears as "Shruthy Suresh Aggarwal" in… (more)

▼

The student had requested that her name appear as "Shruthy Suresh" on the ETD record even though her name appears as "Shruthy Suresh Aggarwal" in official graduation records and on her diploma.

Large-scale sequencing studies have comprehensively identified genomic alterations in human cancers, but they lack the ability to distinguish between cancer driver and passenger mutations. Unbiased genetic screens are a complementary approach for identifying novel cancer driving genes and establishing functional significance of clinically observed mutations. My thesis projects demonstrate how powerful this approach can be in identifying novel therapeutic targets for human cancer treatment. Recent studies have demonstrated that human lung cancer cells express high levels of Programmed Death Ligand 1 (PD-L1), a ligand of the Programmed Death 1 (PD-1) receptor on T-cells, which allows them to directly suppress T-cell proliferation and function. Monoclonal antibodies disrupting this pathway have yielded remarkable clinical results. However, the mechanisms of PD-L1 regulation in tumor cells remain incompletely understood. I used CRISPR-based screening to identify novel regulators of PD-L1 in human lung cancer cells, revealing potent induction of PD-L1 upon disruption of the heme biosynthesis pathway. Impairment of heme production activates the Integrated Stress Response (ISR), allowing bypass of inhibitory upstream open reading frames in the PD-L1 5'UTR, resulting in enhanced PD-L1 translation and immune suppression. I further demonstrated that ISR-dependent translation of PD-L1 requires the translation initiation factor EIF5B. EIF5B overexpression, which is frequent in human lung cancers and is associated with poor prognosis, is sufficient to induce PD-L1. These findings uncover a new mechanism of immune checkpoint activation and suggest novel targets for therapeutic intervention. Additionally, I have also worked on characterizing Steroid Receptor Coactivator-2, previously identified from a forward genetics screen, as a tumor suppressor in MYC-mediated liver tumorigenesis.

Advisors/Committee Members: Zhu, Hao, O'Donnell, Kathryn A., Brekken, Rolf A., Kliewer, Steven A..

Subjects/Keywords: B7-H1 Antigen; Immunotherapy; Lung Neoplasms; Programmed Cell Death 1 Receptor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suresh, S. (2019). The Integrated Stress Response Pathway Regulates PD-L1 Translation in Human Lung Cancer. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/6624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suresh, Shruthy. “The Integrated Stress Response Pathway Regulates PD-L1 Translation in Human Lung Cancer.” 2019. Thesis, University of Texas Southwestern Medical Center. Accessed April 13, 2021. http://hdl.handle.net/2152.5/6624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suresh, Shruthy. “The Integrated Stress Response Pathway Regulates PD-L1 Translation in Human Lung Cancer.” 2019. Web. 13 Apr 2021.

Vancouver:

Suresh S. The Integrated Stress Response Pathway Regulates PD-L1 Translation in Human Lung Cancer. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2019. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2152.5/6624.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suresh S. The Integrated Stress Response Pathway Regulates PD-L1 Translation in Human Lung Cancer. [Thesis]. University of Texas Southwestern Medical Center; 2019. Available from: http://hdl.handle.net/2152.5/6624

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

18. Selck, Claudia. New approaches to induce tolerance in autoantigen-specific memory T cells in type 1 diabetes.

Degree: 2018, University of Melbourne

URL: http://hdl.handle.net/11343/220001

► Autoimmune disorders like type 1 diabetes (T1D) result from the failure of immune tolerance mechanisms. Antigen-specific therapy constitutes an attractive approach to re-establish a tolerant… (more)

▼ Autoimmune disorders like type 1 diabetes (T1D) result from the failure of immune tolerance mechanisms. Antigen-specific therapy constitutes an attractive approach to re-establish a tolerant state but has so far not been successful in clinical settings. Importantly, treatment after the onset of autoimmunity is likely to be less effective due to established autoimmune responses and active inflammation in the islets. Moreover, a major hurdle might be the persistence of antigen-experienced islet-reactive T cells and the induction of tolerance in these memory cells is challenging. Hence, therapies intended to induce antigen-specific tolerance may need additional immunomodulatory treatments to be effective in individuals with established autoimmunity. Here, we aimed to identify a combination of interventions that induces tolerance in antigen-specific memory T cells. For this study, we generated non-obese diabetic (NOD) mice with tetracycline-regulated expression of islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) in antigen presenting cells (TII mice) and tracked IGRP-specific CD8+ T cells using tetramer enrichment. Notably, while naïve IGRP-specific T cells are eliminated upon antigen expression, memory T cells are refractory to cell depletion. Interestingly, these memory T cells up-regulated several exhaustion markers in response to antigen exposure but showed no functional impairment. We hypothesized that combining short-term treatment with non-Fc-binding anti-CD3 F(ab’)2 and the co-stimulation blocker abatacept together with antigen expression might induce effective tolerance in TII mice. However, after initial depletion of antigen-experienced IGRP-specific cells by anti-CD3 F(ab’)2, these cells re-expanded and were still functional. Thus, our combination approach has only limited efficacy in established autoimmunity. Combination therapies of antigen-specific therapies with agents that support the induction of complete T cell exhaustion might be more successful and should be tested in future studies.

Subjects/Keywords: Type 1 diabetes; tolerance; antigen; memory T cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Selck, C. (2018). New approaches to induce tolerance in autoantigen-specific memory T cells in type 1 diabetes. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/220001

Chicago Manual of Style (16^th Edition):

Selck, Claudia. “New approaches to induce tolerance in autoantigen-specific memory T cells in type 1 diabetes.” 2018. Doctoral Dissertation, University of Melbourne. Accessed April 13, 2021. http://hdl.handle.net/11343/220001.

MLA Handbook (7^th Edition):

Selck, Claudia. “New approaches to induce tolerance in autoantigen-specific memory T cells in type 1 diabetes.” 2018. Web. 13 Apr 2021.

Vancouver:

Selck C. New approaches to induce tolerance in autoantigen-specific memory T cells in type 1 diabetes. [Internet] [Doctoral dissertation]. University of Melbourne; 2018. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/11343/220001.

Council of Science Editors:

Selck C. New approaches to induce tolerance in autoantigen-specific memory T cells in type 1 diabetes. [Doctoral Dissertation]. University of Melbourne; 2018. Available from: http://hdl.handle.net/11343/220001

Georgia Tech

19. Li, Kaitao. 2D kinetic study of of PD-1 interaction and its inhibition of T-cell antigen recognition.

Degree: PhD, Biomedical Engineering (Joint GT/Emory Department), 2016, Georgia Tech

URL: http://hdl.handle.net/1853/58608

► Programmed death-1 (PD-1) is an immune-checkpoint receptor with its primary function to maintain peripheral tolerance of the adaptive immune responses. The importance of PD-1 is… (more)

▼ Programmed death-1 (PD-1) is an immune-checkpoint receptor with its primary function to maintain peripheral tolerance of the adaptive immune responses. The importance of PD-1 is evidenced by its deficiency leading to autoimmune disorders, its central role in the identification and restoration of the exhausted phenotypes of antigen-specific T cells, and the great success in targeting this pathway for cancer immunotherapy. To better understand the fundamental question as how PD-1 achieves the potent but well-controlled inhibition, we applied kinetic approaches focusing on its in situ ligand binding characteristics, and the early impact on antigen recognition by the T cell receptor (TCR) and coreceptor CD8. Different from the weak three-dimensional (3D) affinities measured in solution using purified PD-1 and ligands, the two-dimensional (2D) affinities of ligand binding to mouse and human PD-1 expressed on cell membrane span a range from middle to strong, whereas PD-L1–B7-1 binding is much weaker. Comparison of 2D and 3D affinities of PD-1 with B7-1–CD28 and B7-1–cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) as well as others reveals distinct kinetic mechanisms underlying the inhibition of PD-1 and CTLA-4, and differential enhancement of in situ ligand binding for various receptors by the cellular environment. By integrating the 2D kinetic analysis of PD-1 with TCR and CD8, we probed an apparent “negative cooperativity” between these two axis, manifested as reduced molecular bond number and bond lifetime when respective ligands were co-presented. Examination with force spectroscopy suggested the “negative cooperativity” to be the net outcome of suppressed “positive cooperativity” between TCR and CD8. Moreover, the dependence of this suppression on Src homology region 2 domain-containing phosphatase-2 (SHP-2) and lymphocyte-specific protein tyrosine kinase (Lck) further identified it as a “binding-signaling-binding” feedback mechanism representing fine-tuning of antigen recognition by costimulatory/coinhibitory receptors via targeting the TCR–CD8 machinery. In situ kinetic analysis also indicated the existence of a novel binding partner for human PD-L1, which was identified and validated to be CD222. The hPD-L1–CD222 interaction consists of both protein-protein and lectin-carbohydrate binding components, and is stronger than hPD-L1–PD-1 according to its higher 3D and 2D affinity/avidity. Most importantly, CD222 is upregulated on the plasma membrane of activated T cells and competes with PD-1 for hPD-L1, suggesting potentially significant functions on T cells at least in part by perturbing the hPD-L1–PD-1 interaction. Overall, our results provide an in depth understanding of the in situ interaction and function of PD-1, and uncover a novel interaction of hPD-L1–CD222, highlighting the complexity and significance of costimulatory/coinhibitory molecules in modulating T cell responses. Advisors/Committee Members: Zhu, Cheng (advisor), Davis, Simon (committee member), Grakoui, Arash (committee member), Kemp, Melissa (committee member), Thomas, Susan (committee member).

Subjects/Keywords: Programmed death-1; 2D kinetics; T-cell antigen recognition; CD8, CD222

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, K. (2016). 2D kinetic study of of PD-1 interaction and its inhibition of T-cell antigen recognition. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/58608

Chicago Manual of Style (16^th Edition):

Li, Kaitao. “2D kinetic study of of PD-1 interaction and its inhibition of T-cell antigen recognition.” 2016. Doctoral Dissertation, Georgia Tech. Accessed April 13, 2021. http://hdl.handle.net/1853/58608.

MLA Handbook (7^th Edition):

Li, Kaitao. “2D kinetic study of of PD-1 interaction and its inhibition of T-cell antigen recognition.” 2016. Web. 13 Apr 2021.

Vancouver:

Li K. 2D kinetic study of of PD-1 interaction and its inhibition of T-cell antigen recognition. [Internet] [Doctoral dissertation]. Georgia Tech; 2016. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1853/58608.

Council of Science Editors:

Li K. 2D kinetic study of of PD-1 interaction and its inhibition of T-cell antigen recognition. [Doctoral Dissertation]. Georgia Tech; 2016. Available from: http://hdl.handle.net/1853/58608

20. Huschke, Allison M. Hemostatic responses to exercise in a polycythemia vera patient.

Degree: 2016, James Madison University

URL: https://commons.lib.jmu.edu/honors201019/247

► PURPOSE: To assess the hemostatic responses to exercise in a patient with Polycythemia Vera (PV). METHODS: Six female runners (≥15 miles/week) completed a maximal treadmill… (more)

Subjects/Keywords: tPA-antigen; PAI-1; Factor VIII; hematocrit; Sports Sciences

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APA (6^th Edition):

Huschke, A. M. (2016). Hemostatic responses to exercise in a polycythemia vera patient. (Masters Thesis). James Madison University. Retrieved from https://commons.lib.jmu.edu/honors201019/247

Chicago Manual of Style (16^th Edition):

Huschke, Allison M. “Hemostatic responses to exercise in a polycythemia vera patient.” 2016. Masters Thesis, James Madison University. Accessed April 13, 2021. https://commons.lib.jmu.edu/honors201019/247.

MLA Handbook (7^th Edition):

Huschke, Allison M. “Hemostatic responses to exercise in a polycythemia vera patient.” 2016. Web. 13 Apr 2021.

Vancouver:

Huschke AM. Hemostatic responses to exercise in a polycythemia vera patient. [Internet] [Masters thesis]. James Madison University; 2016. [cited 2021 Apr 13]. Available from: https://commons.lib.jmu.edu/honors201019/247.

Council of Science Editors:

Huschke AM. Hemostatic responses to exercise in a polycythemia vera patient. [Masters Thesis]. James Madison University; 2016. Available from: https://commons.lib.jmu.edu/honors201019/247

Lehigh University

21. Plucinsky, Sarah. The Biophysical Characterization of Caveolin-1.

Degree: PhD, Chemistry, 2018, Lehigh University

URL: https://preserve.lehigh.edu/etd/4314

► The main topic of this doctoral dissertation is the biophysical characterization of caveolin-1. Caveolin-1 is an integral membrane protein that has been shown to be… (more)

Subjects/Keywords: Caveolae; Caveolin-1; Membrane protein Structure; Biochemistry

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APA (6^th Edition):

Plucinsky, S. (2018). The Biophysical Characterization of Caveolin-1. (Doctoral Dissertation). Lehigh University. Retrieved from https://preserve.lehigh.edu/etd/4314

Chicago Manual of Style (16^th Edition):

Plucinsky, Sarah. “The Biophysical Characterization of Caveolin-1.” 2018. Doctoral Dissertation, Lehigh University. Accessed April 13, 2021. https://preserve.lehigh.edu/etd/4314.

MLA Handbook (7^th Edition):

Plucinsky, Sarah. “The Biophysical Characterization of Caveolin-1.” 2018. Web. 13 Apr 2021.

Vancouver:

Plucinsky S. The Biophysical Characterization of Caveolin-1. [Internet] [Doctoral dissertation]. Lehigh University; 2018. [cited 2021 Apr 13]. Available from: https://preserve.lehigh.edu/etd/4314.

Council of Science Editors:

Plucinsky S. The Biophysical Characterization of Caveolin-1. [Doctoral Dissertation]. Lehigh University; 2018. Available from: https://preserve.lehigh.edu/etd/4314

22. Brodovitch, Alexandre. Détection et première analyse d'antigènes par les lymphocytes T : Antigen detection and initial analysis by T lymphocytes.

Degree: Docteur es, Immunologie, 2014, Aix Marseille Université

URL: http://www.theses.fr/2014AIXM4050

►

Les lymphocytes T (LT) doivent analyser efficacement un nombre important de cellules présentatrices d'antigène (CPA) afin de détecter un antigène spécifique et d'initier une réponse… (more)

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Les lymphocytes T (LT) doivent analyser efficacement un nombre important de cellules présentatrices d'antigène (CPA) afin de détecter un antigène spécifique et d'initier une réponse immunitaire adaptée. La reconnaissance par le récepteur des cellules T (TCR) d'un peptide spécifique associé au complexe majeur d'histocompatibilité (pMHC) doit donc être extrêmement sensible et rapide. Alors que les voies de signalisation en aval du TCR sont largement étudiées, la cinétique de la discrimination des ligands par le TCR et de l'activation initiale du LT reste mal connue. Des surfaces solides recouvertes de ligands du TCR nous ont permis d'étudier les événements cellulaires initiés par la détection d'un antigène. L'utilisation de la microscopie a fluorescence par réflexion totale interne (TIRFM) et la microscopie interférentielle (IRM) nous a permis de montrer que l'interaction initiale entre surface et LT est attribuable à des microvillosités mobiles. La stimulation du TCR active en quelques secondes un mouvement de rétraction de ces microvillosités. Ces mouvements actifs sont régulés par l'activation de la PLC- γ1 et sont dépendants des myosines (IIA, Ic et Ig), de la cofiline et de l'ezrine. Après cette phase d'analyse de l'environnement extracellulaire, la stimulation du TCR entraîne l'étalement rapide et actif de la cellule. L'amplitude et la vitesse d'étalement reflètent l'efficacité activatrice du ligand reconnu par le TCR. En moins de 5 minutes différents pMHC, ayant des propriétés biophysiques proches, sont donc discriminés par les LT et capables d'induire une première réponse cellulaire spécifique.

T lymphocytes need to efficiently probe a large number of antigen-presenting cell (APC) in order to detect an agonist antigen and to initiate an effective immune response. Therefore, engagement of the T-cell receptor (TCR) by peptide-bound major histocompatibility complex (pMHC) is a highly sensitive and rapid process. While signaling pathways downstream the TCR are extensively studied, little is known about antigen discrimination kinetic and initial T-cell response.Model planar surfaces coated with TCR ligand allowed us to study cellular events initiated by antigen detection. Using TIRFM (total internal reflexion microscopy) and IRM (interference reflexion microscopy) we showed that T-cell initial contacts with the surface were mediated by mobile microvilli. TCR engagement triggers a pulling motion of T cell surface microvilli within seconds. Active microvilli movements are dependent on PLC-γ1 activation and on the presence of myosins (IIA, Ic and Ig) as well as cofilin and ezrin. After this extracellular environment probing period, TCR stimulation triggered a rapid and active cell spreading. Cell spreading amplitude and speed reflect the ligand activating efficacity. In less than 5 minutes pMHC with different biophysical properties were discriminated by T-cells and triggered a first specific cellular response.

Advisors/Committee Members: Bongrand, Pierre (thesis director).

Subjects/Keywords: Lymphocyte T; Détection des Antigènes; Étalement; Mouvements de membrane; Tirfm; Irm; T-Lymphocyte; Antigen detection; Spreading; Membrane movement; Tirfm; Irm; 571

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brodovitch, A. (2014). Détection et première analyse d'antigènes par les lymphocytes T : Antigen detection and initial analysis by T lymphocytes. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2014AIXM4050

Chicago Manual of Style (16^th Edition):

Brodovitch, Alexandre. “Détection et première analyse d'antigènes par les lymphocytes T : Antigen detection and initial analysis by T lymphocytes.” 2014. Doctoral Dissertation, Aix Marseille Université. Accessed April 13, 2021. http://www.theses.fr/2014AIXM4050.

MLA Handbook (7^th Edition):

Brodovitch, Alexandre. “Détection et première analyse d'antigènes par les lymphocytes T : Antigen detection and initial analysis by T lymphocytes.” 2014. Web. 13 Apr 2021.

Vancouver:

Brodovitch A. Détection et première analyse d'antigènes par les lymphocytes T : Antigen detection and initial analysis by T lymphocytes. [Internet] [Doctoral dissertation]. Aix Marseille Université 2014. [cited 2021 Apr 13]. Available from: http://www.theses.fr/2014AIXM4050.

Council of Science Editors:

Brodovitch A. Détection et première analyse d'antigènes par les lymphocytes T : Antigen detection and initial analysis by T lymphocytes. [Doctoral Dissertation]. Aix Marseille Université 2014. Available from: http://www.theses.fr/2014AIXM4050

23. Brocqueville, Guillaume. Dualité fonctionnelle de LMP1 : implication dans l’apoptose et la transformation cellulaire : Functionnal duality of LMP1 : involvement in apoptosis and cellular transformation.

Degree: Docteur es, Biochimie et biologie moléculaire, 2011, Université Lille II – Droit et Santé

URL: http://www.theses.fr/2011LIL2S030

►

Le virus d’Epstein-Barr (EBV) est un herpèsvirus humain qui infecte plus de 90% de la population généralement de façon bénigne et asymptomatique. Cependant, de nombreuses… (more)

▼

Le virus d’Epstein-Barr (EBV) est un herpèsvirus humain qui infecte plus de 90% de la population généralement de façon bénigne et asymptomatique. Cependant, de nombreuses données démontrent que ce virus peut également contribuer à certains processus de cancérisation. En effet, l’EBV est associé à de nombreuses pathologies malignes telles que le lymphome de Burkitt, le lymphome hodgkinien et le carcinome du nasopharynx. Dans la grande majorité de ces cancers associées à ce virus, l’EBV exprime un programme de latence de type II durant lequel la protéine LMP1 est exprimée. Elle est décrite comme l’oncogène majeur de l’EBV car son expression est nécessaire à la survie et à la prolifération des lignées transformées in vitro. Cette protéine membranaire est fonctionnellement apparentée aux membres de la famille des récepteurs du TNF. LMP1 est constitutivement active et son expression conduit à l’activation de voies de signalisation telles que les voies NF-κB, PI3K et des MAPK. L’activation de ces voies de signalisation cellulaire confère à LMP1 des propriétés oncogéniques, cependant, des effets toxiques liés à son expression ont également été décrits. Effectivement, LMP1 est capable d’induire l’apoptose dans différents types cellulaires. Dans ce contexte, nous avons d’abord développé et caractérisé, des variants dérivés de LMP1 constitués de sa partie C-terminale signalisatrice, complète ou partielle, fusionnée à la protéine GFP. Nous montrons que ces variants sont capables de séquestrer les protéines adaptatrices se fixant à LMP1 ou au récepteur TNFR1, et d’inhiber le signal et les phénotypes induits par ces derniers. Ces protéines à effet dominant négatif peuvent ainsi contrecarrer les effets transformants de LMP1 dans des modèles de latence II et III. Ces dominants négatifs peuvent aussi inhiber l’activation du TNFR1 et les phénotypes qui en découlent. Puis, nous avons étudié les propriétés de LMP1 en dehors d’un contexte infectieux et son rôle dans la transformation épithéliale. Nous démontrons que LMP1 induit la mort des cellules épithéliales MDCK mais certaines cellules outrepassent ses effets cytotoxiques générant des lignées qui expriment stablement LMP1 et dans lesquelles cet oncogène viral favorise la survie et exacerbe les phénotypes induits par le facteur de croissance HGF. Le caractère ambivalent de LMP1 pourrait limiter le pouvoir oncogène de l’EBV mais en contrepartie favoriser l’émergence de cellules résistantes à l’apoptose et capables de répondre de façon accrue à des facteurs de croissance. Nos travaux ont permis de mieux comprendre la dualité fonctionnelle de LMP1, d’une part ses effets oncogènes favorisant la survie cellulaire et d’autre part ses propriétés pro-apoptotiques, induites directement ou révélées suite à son inhibition, limitant la tumorigenèse. La caractérisation des mécanismes moléculaires impliquant LMP1 pourrait ainsi participer à la définition de potentielles stratégies thérapeutiques pour le traitement de cancers associés à l’EBV et où LMP1 est exprimée.

Epstein-Barr virus…

Advisors/Committee Members: Adriaenssens, Éric (thesis director).

Subjects/Keywords: Survie cellulaire; LMP-1 (Latent membrane Protein-1); Cell survival

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APA (6^th Edition):

Brocqueville, G. (2011). Dualité fonctionnelle de LMP1 : implication dans l’apoptose et la transformation cellulaire : Functionnal duality of LMP1 : involvement in apoptosis and cellular transformation. (Doctoral Dissertation). Université Lille II – Droit et Santé. Retrieved from http://www.theses.fr/2011LIL2S030

Chicago Manual of Style (16^th Edition):

Brocqueville, Guillaume. “Dualité fonctionnelle de LMP1 : implication dans l’apoptose et la transformation cellulaire : Functionnal duality of LMP1 : involvement in apoptosis and cellular transformation.” 2011. Doctoral Dissertation, Université Lille II – Droit et Santé. Accessed April 13, 2021. http://www.theses.fr/2011LIL2S030.

MLA Handbook (7^th Edition):

Brocqueville, Guillaume. “Dualité fonctionnelle de LMP1 : implication dans l’apoptose et la transformation cellulaire : Functionnal duality of LMP1 : involvement in apoptosis and cellular transformation.” 2011. Web. 13 Apr 2021.

Vancouver:

Brocqueville G. Dualité fonctionnelle de LMP1 : implication dans l’apoptose et la transformation cellulaire : Functionnal duality of LMP1 : involvement in apoptosis and cellular transformation. [Internet] [Doctoral dissertation]. Université Lille II – Droit et Santé 2011. [cited 2021 Apr 13]. Available from: http://www.theses.fr/2011LIL2S030.

Council of Science Editors:

Brocqueville G. Dualité fonctionnelle de LMP1 : implication dans l’apoptose et la transformation cellulaire : Functionnal duality of LMP1 : involvement in apoptosis and cellular transformation. [Doctoral Dissertation]. Université Lille II – Droit et Santé 2011. Available from: http://www.theses.fr/2011LIL2S030

NSYSU

24. Lin, Tung-cheng. Antiserum titer determination and adherence comparison of three major outer membrane proteins TSA56, TSA47 and TSA22 in Orientia tsutsugamushi.

Degree: Master, Biological Sciences, 2011, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0907111-024719

► Orientia tsutsugamushi, the causative agent of scrub typhus, is an obligate intracellular pathogen. Recent studies show that the complete genome sequence of Orientia tsutsugamushi have… (more)

Subjects/Keywords: antiserum immunoblots; outer membrane protein; type-specific antigen; Orientia tsutsugamushi; scrub typhus; adhesion assay

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APA (6^th Edition):

Lin, T. (2011). Antiserum titer determination and adherence comparison of three major outer membrane proteins TSA56, TSA47 and TSA22 in Orientia tsutsugamushi. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0907111-024719

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Tung-cheng. “Antiserum titer determination and adherence comparison of three major outer membrane proteins TSA56, TSA47 and TSA22 in Orientia tsutsugamushi.” 2011. Thesis, NSYSU. Accessed April 13, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0907111-024719.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Tung-cheng. “Antiserum titer determination and adherence comparison of three major outer membrane proteins TSA56, TSA47 and TSA22 in Orientia tsutsugamushi.” 2011. Web. 13 Apr 2021.

Vancouver:

Lin T. Antiserum titer determination and adherence comparison of three major outer membrane proteins TSA56, TSA47 and TSA22 in Orientia tsutsugamushi. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 Apr 13]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0907111-024719.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin T. Antiserum titer determination and adherence comparison of three major outer membrane proteins TSA56, TSA47 and TSA22 in Orientia tsutsugamushi. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0907111-024719

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Washington State University

25. [No author]. PROSTATE CANCER CELL CAPTURE USING IMMOBILIZED INHIBITORS OF PROSTATE-SPECIFIC MEMBRANE ANTIGEN .

Degree: 2011, Washington State University

URL: http://hdl.handle.net/2376/3543

► Prostate cancer, especially its metastasis to the bone, is the leading cause of death for men in the US. The development of bone metastasis is… (more)

▼ Prostate cancer, especially its metastasis to the bone, is the leading cause of death for men in the US. The development of bone metastasis is a complex process that is potentially established by prostate circulating tumor cancers (PCTCs) through the lymphatic system and the blood circulation system. These PCTCs in the circulation represent an intrinsic property of the tumor and can provide unique information related to the nature of the prostate tumors. Unfortunately, current information that is gathered for detecting, isolating, and enriching PCTCs, which based on the epithelial cell adhesion molecule (EpCAM) as a biomarker, is limited and controversial. The main reason is that the invasive PCTCs lose the EpCAM antigens during the epithelial mesenchymal transition (EMT) in the metastatic process. On the other hand, prostate-specific membrane antigen (PSMA), has been recognized as the most well-established biomarker for prostate cancer. Its utilization as a novel target in both diagnostic and therapeutic interventions is intriguing. However, there are limited amounts of research on its usages as a validated biomarker in PCTC isolation, enrichment and detection. Herein, the thesis will focus on two studies, which are the detection of prostate tumor cells using chemoafinity labels and cell capture using immobilize inhibitor of PSMA. We have successfully demonstrated the specific binding to PSMA on prostate cancer cells by a fluorescent phosphoramidate PSMA inhibitor and the quantification and detection of prostate cancer cells in blood by flow cytometry. In addition, we have illustrated that the irreversible PSMA inhibitor biotin-PEG12-CTT-54 can effectively serve as a pre-targeting bait to capture of PSMA+ cells using stretptavidin coated magnetic beads. High selectivity, recovery, and viability was achieved for the capture of PSMA+ cells in both model experiments with mixtures of LNCaP cells and WBCs as well as blood samples spiked with LNCaP cells. More importantly, captured cells could be subsequently propagated in vitro. This methodology for the detection, isolation, and culture of PCTCs from peripheral blood can serve as an effective tool for the detection of metastatic prostate cancer, treatment monitoring, and the development of personalized therapy based on the responsiveness of PCTCs to chemotherapeutic strategies. Advisors/Committee Members: Berkman, Clifford E (advisor).

Subjects/Keywords: Chemistry; circulating tumor cells; flow cytometry; magnetic beads; Prostate cancer; prostate-specific membrane antigen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2011). PROSTATE CANCER CELL CAPTURE USING IMMOBILIZED INHIBITORS OF PROSTATE-SPECIFIC MEMBRANE ANTIGEN . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/3543

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “PROSTATE CANCER CELL CAPTURE USING IMMOBILIZED INHIBITORS OF PROSTATE-SPECIFIC MEMBRANE ANTIGEN .” 2011. Thesis, Washington State University. Accessed April 13, 2021. http://hdl.handle.net/2376/3543.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “PROSTATE CANCER CELL CAPTURE USING IMMOBILIZED INHIBITORS OF PROSTATE-SPECIFIC MEMBRANE ANTIGEN .” 2011. Web. 13 Apr 2021.

Vancouver:

author] [. PROSTATE CANCER CELL CAPTURE USING IMMOBILIZED INHIBITORS OF PROSTATE-SPECIFIC MEMBRANE ANTIGEN . [Internet] [Thesis]. Washington State University; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2376/3543.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. PROSTATE CANCER CELL CAPTURE USING IMMOBILIZED INHIBITORS OF PROSTATE-SPECIFIC MEMBRANE ANTIGEN . [Thesis]. Washington State University; 2011. Available from: http://hdl.handle.net/2376/3543

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. [No author]. The Evolution of Phosphoramidates from Small-Molecule Inhibitors to Tunable Cleavable Linkers .

Degree: 2016, Washington State University

URL: http://hdl.handle.net/2376/12074

► During the development of a series of phosphoramidate-based inhibitors to prostate-specific membrane antigen, we observed a trend in increasing acid stability as the distance between… (more)

Subjects/Keywords: Organic chemistry; Cleavable linker; Drug conjugate; Drug delivery; Phosphoramidate; pH-tunable; Prostate-specific membrane antigen

14 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2016). The Evolution of Phosphoramidates from Small-Molecule Inhibitors to Tunable Cleavable Linkers . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/12074

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “The Evolution of Phosphoramidates from Small-Molecule Inhibitors to Tunable Cleavable Linkers .” 2016. Thesis, Washington State University. Accessed April 13, 2021. http://hdl.handle.net/2376/12074.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “The Evolution of Phosphoramidates from Small-Molecule Inhibitors to Tunable Cleavable Linkers .” 2016. Web. 13 Apr 2021.

Vancouver:

author] [. The Evolution of Phosphoramidates from Small-Molecule Inhibitors to Tunable Cleavable Linkers . [Internet] [Thesis]. Washington State University; 2016. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2376/12074.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. The Evolution of Phosphoramidates from Small-Molecule Inhibitors to Tunable Cleavable Linkers . [Thesis]. Washington State University; 2016. Available from: http://hdl.handle.net/2376/12074

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. DAI XILEI. Differential Signal Induction, Membrane Trafficking and Immune Effector Mediated by FcyRl Versus FcyRlla.

Degree: 2009, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/160955

Subjects/Keywords: Fcgamma receptors; Membrane trafficking; Antigen presentation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

XILEI, D. (2009). Differential Signal Induction, Membrane Trafficking and Immune Effector Mediated by FcyRl Versus FcyRlla. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/160955

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

XILEI, DAI. “Differential Signal Induction, Membrane Trafficking and Immune Effector Mediated by FcyRl Versus FcyRlla.” 2009. Thesis, National University of Singapore. Accessed April 13, 2021. https://scholarbank.nus.edu.sg/handle/10635/160955.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

XILEI, DAI. “Differential Signal Induction, Membrane Trafficking and Immune Effector Mediated by FcyRl Versus FcyRlla.” 2009. Web. 13 Apr 2021.

Vancouver:

XILEI D. Differential Signal Induction, Membrane Trafficking and Immune Effector Mediated by FcyRl Versus FcyRlla. [Internet] [Thesis]. National University of Singapore; 2009. [cited 2021 Apr 13]. Available from: https://scholarbank.nus.edu.sg/handle/10635/160955.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

XILEI D. Differential Signal Induction, Membrane Trafficking and Immune Effector Mediated by FcyRl Versus FcyRlla. [Thesis]. National University of Singapore; 2009. Available from: https://scholarbank.nus.edu.sg/handle/10635/160955

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

28. Neek, Medea Babaie. Protein-Based Nanoparticles for Cancer Immunotherapy.

Degree: Chemical and Biochemical Engineering, 2019, University of California – Irvine

URL: http://www.escholarship.org/uc/item/0nq864zk

► Although progress has been made in conventional cancer therapy, cancer is still the second leading cause of death in the United States. Recently, a new… (more)

▼ Although progress has been made in conventional cancer therapy, cancer is still the second leading cause of death in the United States. Recently, a new approach for cancer treatment known as immunotherapy has shown remarkable success. Within immunotherapy, cancer vaccines train the body to recognize tumor-associated antigens for targeted destruction of cancer cells. While promising, clinical success of cancer vaccines to date has been limited. Our work focuses on utilizing a viral-mimetic design to develop a new platform to improve cancer vaccine efficacy. We have been exploring the non-viral E2 protein nanoparticle as a cancer vaccine platform. We verified that simultaneous delivery of cancer antigen epitopes (e.g., gp100, NY-ESO-1, MAGE-A3) and adjuvant (CpG) within E2 nanoparticles resulted in improved anti-tumor responses. Prophylactic immunization with CpG-gp-E2 (E2 conjugated with gp100 and CpG) increased animal survival time by ̴ 40% in an aggressive tumor model. Furthermore, we demonstrated that simultaneous delivery of human-restricted cancer-testis epitopes and CpG within E2 (CpG-NYESO-E2 and CpG-MAGE-E2) resulted in an increase in IFN-γ secretion and enhanced lytic activity towards human cancer cells expressing the antigen. These results demonstrate the broad efficacy of the E2 nanoparticle platform against various target cancer antigens.One of the promising new FDA-approved approaches in cancer immunotherapy is the obstruction of inhibitory effects of immune checkpoint molecules (e.g., PD-1). However, treatments with checkpoint inhibitors are still not effective in a significant portion of patients. To address this, we examined the therapeutic effects of combination delivery of anti-PD-1 with CpG-gp-E2 nanoparticles. In the B16-F10 melanoma tumor model, ̴ 50% of the mice treated with combination therapy remained tumor-free, compared with 0% and ̴ 5% survival for vaccine and anti-PD-1 treatments alone, respectively. Cell uptake of the E2 nanoparticle in vitro was also investigated, and we demonstrated that surface display of CpG on E2 increased the nanoparticle uptake by APCs, which can potentially further increase the vaccine efficacy. Altogether, our results demonstrate the potential of the E2 protein nanoparticle as an effective cancer vaccine platform for inducing anti-tumor responses. These findings could lead to more effective cancer treatments.

Subjects/Keywords: Immunology; Chemical engineering; Animal Survival; anti-PD-1; Cancer Antigen; Cancer Vaccine; Nanoparticles; Vaccine platform

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Neek, M. B. (2019). Protein-Based Nanoparticles for Cancer Immunotherapy. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/0nq864zk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Neek, Medea Babaie. “Protein-Based Nanoparticles for Cancer Immunotherapy.” 2019. Thesis, University of California – Irvine. Accessed April 13, 2021. http://www.escholarship.org/uc/item/0nq864zk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Neek, Medea Babaie. “Protein-Based Nanoparticles for Cancer Immunotherapy.” 2019. Web. 13 Apr 2021.

Vancouver:

Neek MB. Protein-Based Nanoparticles for Cancer Immunotherapy. [Internet] [Thesis]. University of California – Irvine; 2019. [cited 2021 Apr 13]. Available from: http://www.escholarship.org/uc/item/0nq864zk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Neek MB. Protein-Based Nanoparticles for Cancer Immunotherapy. [Thesis]. University of California – Irvine; 2019. Available from: http://www.escholarship.org/uc/item/0nq864zk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

29. Liu, Feiyang (Victoria). Antigen-binding Fragments: Production for and Use in Crystallographic Studies.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/43090

►

An immunoglobulin (IgG) consists of two antigen-binding fragments (Fab) connected to a crystallisable fragment through hinge regions. This thesis mainly investigates the production methods of… (more)

Subjects/Keywords: antigen-binding fragment; X-ray crystallography; HIV-1; broadly neutralizing monoclonal antibody; 0307

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, F. (. (2013). Antigen-binding Fragments: Production for and Use in Crystallographic Studies. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/43090

Chicago Manual of Style (16^th Edition):

Liu, Feiyang (Victoria). “Antigen-binding Fragments: Production for and Use in Crystallographic Studies.” 2013. Masters Thesis, University of Toronto. Accessed April 13, 2021. http://hdl.handle.net/1807/43090.

MLA Handbook (7^th Edition):

Liu, Feiyang (Victoria). “Antigen-binding Fragments: Production for and Use in Crystallographic Studies.” 2013. Web. 13 Apr 2021.

Vancouver:

Liu F(. Antigen-binding Fragments: Production for and Use in Crystallographic Studies. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1807/43090.

Council of Science Editors:

Liu F(. Antigen-binding Fragments: Production for and Use in Crystallographic Studies. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/43090

Boston University

30. Randhawa, Anantbir. A reduction in the RNA binding protein TIA1 protects against neurodegeneration, rescues behavioral deficits and prolongs survival.

Degree: MS, Medical Sciences, 2018, Boston University

URL: http://hdl.handle.net/2144/31265

► RNA binding proteins (RBPs) have been found to be frequently involved in neurodegenerative diseases (Ash 2014). Mutations in RBPs cause amyotrophic lateral sclerosis (ALS), spinocerebellar… (more)

Subjects/Keywords: Medicine; Neurodegeneration; Neuroinflammation; Stress granules; Tau; Tauopathies; T-cell intracellular antigen 1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Randhawa, A. (2018). A reduction in the RNA binding protein TIA1 protects against neurodegeneration, rescues behavioral deficits and prolongs survival. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/31265

Chicago Manual of Style (16^th Edition):

Randhawa, Anantbir. “A reduction in the RNA binding protein TIA1 protects against neurodegeneration, rescues behavioral deficits and prolongs survival.” 2018. Masters Thesis, Boston University. Accessed April 13, 2021. http://hdl.handle.net/2144/31265.

MLA Handbook (7^th Edition):

Randhawa, Anantbir. “A reduction in the RNA binding protein TIA1 protects against neurodegeneration, rescues behavioral deficits and prolongs survival.” 2018. Web. 13 Apr 2021.

Vancouver:

Randhawa A. A reduction in the RNA binding protein TIA1 protects against neurodegeneration, rescues behavioral deficits and prolongs survival. [Internet] [Masters thesis]. Boston University; 2018. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/2144/31265.

Council of Science Editors:

Randhawa A. A reduction in the RNA binding protein TIA1 protects against neurodegeneration, rescues behavioral deficits and prolongs survival. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/31265

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Open Access Theses and Dissertations