subject:(Antibodies)

McGill University

1. Pan, Bin-Tao. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.

Degree: PhD, Department of Biochemistry., 1982, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile154006.pdf

►

Sheep reticulocyte surface specific antigens have been isolated by anti-reticulocyte surface specific antibodies. Moreover, the major specific antigen has been identified as the transferrin receptor.… (more)

Subjects/Keywords: Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pan, B. (1982). Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. (Doctoral Dissertation). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile154006.pdf

Chicago Manual of Style (16^th Edition):

Pan, Bin-Tao. “Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.” 1982. Doctoral Dissertation, McGill University. Accessed February 25, 2020. http://digitool.library.mcgill.ca/thesisfile154006.pdf.

MLA Handbook (7^th Edition):

Pan, Bin-Tao. “Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.” 1982. Web. 25 Feb 2020.

Vancouver:

Pan B. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. [Internet] [Doctoral dissertation]. McGill University; 1982. [cited 2020 Feb 25]. Available from: http://digitool.library.mcgill.ca/thesisfile154006.pdf.

Council of Science Editors:

Pan B. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. [Doctoral Dissertation]. McGill University; 1982. Available from: http://digitool.library.mcgill.ca/thesisfile154006.pdf

Boston University

2. Russell, Anthony. Antibodies involved in homograft rejection.

Degree: MA, Biology, 1960, Boston University

URL: http://hdl.handle.net/2144/24569

Subjects/Keywords: Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Russell, A. (1960). Antibodies involved in homograft rejection. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/24569

Chicago Manual of Style (16^th Edition):

Russell, Anthony. “Antibodies involved in homograft rejection.” 1960. Masters Thesis, Boston University. Accessed February 25, 2020. http://hdl.handle.net/2144/24569.

MLA Handbook (7^th Edition):

Russell, Anthony. “Antibodies involved in homograft rejection.” 1960. Web. 25 Feb 2020.

Vancouver:

Russell A. Antibodies involved in homograft rejection. [Internet] [Masters thesis]. Boston University; 1960. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2144/24569.

Council of Science Editors:

Russell A. Antibodies involved in homograft rejection. [Masters Thesis]. Boston University; 1960. Available from: http://hdl.handle.net/2144/24569

University of New South Wales

3. Mi, Yang. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.

Degree: Graduate School of Biomedical Engineering, 2014, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

► Angiogenesis relies on the coordination of endothelial cells, smooth muscle cells and the vascular extracellular matrix around cells. Perlecan is an important heparan sulfate proteoglycan,… (more)

▼ Angiogenesis relies on the coordination of endothelial cells, smooth muscle cells and the vascular extracellular matrix around cells. Perlecan is an important heparan sulfate proteoglycan, which is widely expressed in vascular tissues and essentially involved in angiogenesis. Perlecan exhibits pro- and anti-angiogenic effects differently according to the cell line from which it was derived from and various structures especially its dependent glycosaminoglycans. The aim of the thesis was to generate and characterise monoclonal antibodies against specific domains of perlecan, and investigate their functions in modulating angiogenesis.lmmunopurified human endothelial cell-derived perlecan decorated with heparin sulfate and recombinant perlecan domain V decorated with chondroitin sulfate were characterised by Enzyme linked immunosorbent assay, immunocytochemistry and Western blotting, and then used as antigens for hybridoma screening. There were 205 single clones generated by limiting dilution, while 174 clones were able to recognise endothelial perlecan. 48 clones with high viability and lg production were tested with intact perlecan and Hep Ill treated perlecan, while they showed different immunoreactive changes between two antigens. However, none of the clones reacted with recombinant perlecan domain I or domain V.Of the newly generated monoclonal antibodies (mAbs), 5 mAbs showed potential to modulate the adhesion of endothelial cells and smooth muscle cells, especially 8C8 and 4F2 reduced the adhesion of both cell types. In addition, the 5 mAbs displayed strong immunoreactivity with perlecan derived from HCAECs and HVSMCs in Western blotting and were able to detect the perlecan expressed on two types of cells in immunocytochemistry. In spreading assay, all 5 mAbs were found to inhibit the HVSMC and HCAEC spreading to different degrees. Moreover, the 5 mAbs except 5G8 showed inhibitory effects in HCAEC migration, while only 8C8, 4F7 and 4F2 were able to reduce the migration of HVSMCs. These 5 mAbs could be useful to investigate the presence and structure of perlecan as primary antibodies, since they were able to recognise the perlecan protein core. They could also become potential tools to explain the functions of different domains of perlecan in angiogenesis and developed as immunotherapeutic drugs to mediate angiogenesis in clinical treatments.

Subjects/Keywords: Antibodies; Perlecan

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mi, Y. (2014). Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Mi, Yang. “Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.” 2014. Masters Thesis, University of New South Wales. Accessed February 25, 2020. http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Mi, Yang. “Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.” 2014. Web. 25 Feb 2020.

Vancouver:

Mi Y. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. [Internet] [Masters thesis]. University of New South Wales; 2014. [cited 2020 Feb 25]. Available from: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true.

Council of Science Editors:

Mi Y. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. [Masters Thesis]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

Rutgers University

4. Prabhu, Siddharth, 1995-. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.

Degree: MS, Chemical and Biochemical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

► Monoclonal antibodies are the fastest growing segment of pharmaceutical molecules. Currently, they are used as diagnostics, therapeutics for various medical uses as well as in… (more)

Subjects/Keywords: Monoclonal antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prabhu, Siddharth, 1. (2018). Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

Chicago Manual of Style (16^th Edition):

Prabhu, Siddharth, 1995-. “Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.” 2018. Masters Thesis, Rutgers University. Accessed February 25, 2020. https://rucore.libraries.rutgers.edu/rutgers-lib/59136/.

MLA Handbook (7^th Edition):

Prabhu, Siddharth, 1995-. “Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.” 2018. Web. 25 Feb 2020.

Vancouver:

Prabhu, Siddharth 1. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. [Internet] [Masters thesis]. Rutgers University; 2018. [cited 2020 Feb 25]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/.

Council of Science Editors:

Prabhu, Siddharth 1. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. [Masters Thesis]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

University of KwaZulu-Natal

5. Liao, Hua-Xin. Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual.

Degree: 2011, University of KwaZulu-Natal

URL: http://hdl.handle.net/10413/7884

► The C3-V4 region is a major target of autologous neutralizing antibodies in HIV-1 subtype C infection. We previously identified a Center for AIDS Program of… (more)

Subjects/Keywords: HIV antibodies.; HIV infections – Immunology.; Antibodies, Monoclonal.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liao, H. (2011). Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/7884

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liao, Hua-Xin. “Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual.” 2011. Thesis, University of KwaZulu-Natal. Accessed February 25, 2020. http://hdl.handle.net/10413/7884.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liao, Hua-Xin. “Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual.” 2011. Web. 25 Feb 2020.

Vancouver:

Liao H. Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual. [Internet] [Thesis]. University of KwaZulu-Natal; 2011. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/10413/7884.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liao H. Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual. [Thesis]. University of KwaZulu-Natal; 2011. Available from: http://hdl.handle.net/10413/7884

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

6. Peterson, Lisa K. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.

Degree: PhD, Pathology;, 2007, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

► Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is an autoimmune model of MS, induced by… (more)

▼ Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is an autoimmune model of MS, induced by sensitization with myelin proteins of their encephalitogenic peptides. Sensitization with amino acids 92-106 of myelin oligodendrocyte glycoprotein (MOG) induces different disease courses and neuropathology in A.SW and SJL/J mice. SJL/J mice develop a relapsing-remitting (RR-EAE) disease course characterized by mild demyelinating disease with perivascular T cell infiltration. In contrast, A.SW mice develop a progressive (P-EAE) disease course characterized by large areas of plaque-like demyelination with Ig deposition, polymorphonuclear (PMN) cell infiltration and minimal T cell infiltration and high MOG antibody titers in the serum. This thesis describes, as part of the elucidation of the role of antibody in determining disease course and neuropathology, the generation and characterization of hybridomas producing antibody reactive with MOG92-106. Two MOG92-106 hybridomas produced polyreactive IgM antibodies encoded by immunoglobulin genes in their germline configuration, indicating that the antibodies are natural autoantibodies (NAA). These MOG92-106 reactive NNA bind myelin and cause demyelination in vivo, suggesting that they could contribute to the pathogenesis of P-EAE. In addition, sensitization of A.S.W. mice with MOG92-106 or intraperitoneal injection of the MOG92-106 reactive hybridomas resulted in antibody deposition in glomeruli of kidneys and proteinuria, demonstrating that MOG92-106 reacative NAA can induce systemic autoimmunity. However, depletion of B-1 cells, the main produces of NAA, had minimal effects on the development of demyelination, antibody deposition or disease progression in mice sensitized with MOG92-106. These results indicate that while MOG92-106 reacative NAA are capable of inducing pathology in the CNS and kidneys, they play a minor role in the pathogenesis of MOG92-106-infduced EAE. In addition, this thesis describes induction of another combination of disease course and neuropathology in B10.S mice. B10.S mice have previously been considered resistant to EAE, but sensitization with MOG92-106 resulted in neuropathology in 93% of the mice and monophasic or relapsing-remitting disease course in 30% of the mice. Taken together, this work expands the spectrum of autoimmune disease that can be induced and investigated using MOG92-106.

Subjects/Keywords: Multiple Sclerosis; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peterson, L. K. (2007). Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

Chicago Manual of Style (16^th Edition):

Peterson, Lisa K. “Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.” 2007. Doctoral Dissertation, University of Utah. Accessed February 25, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453.

MLA Handbook (7^th Edition):

Peterson, Lisa K. “Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.” 2007. Web. 25 Feb 2020.

Vancouver:

Peterson LK. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. [Internet] [Doctoral dissertation]. University of Utah; 2007. [cited 2020 Feb 25]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453.

Council of Science Editors:

Peterson LK. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. [Doctoral Dissertation]. University of Utah; 2007. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

Louisiana State University

7. Datta, Shreya. Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus.

Degree: PhD, Life Sciences, 2009, Louisiana State University

URL: etd-04142009-225600 ; https://digitalcommons.lsu.edu/gradschool_dissertations/29

► Vibrio parahaemolyticus is a marine bacterium which is the leading cause of bacterial gastroenteritis due to consumption of raw or undercooked bivalve molluscan shellfish in… (more)

Subjects/Keywords: Vibrio parahaemolyticus; antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Datta, S. (2009). Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-04142009-225600 ; https://digitalcommons.lsu.edu/gradschool_dissertations/29

Chicago Manual of Style (16^th Edition):

Datta, Shreya. “Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus.” 2009. Doctoral Dissertation, Louisiana State University. Accessed February 25, 2020. etd-04142009-225600 ; https://digitalcommons.lsu.edu/gradschool_dissertations/29.

MLA Handbook (7^th Edition):

Datta, Shreya. “Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus.” 2009. Web. 25 Feb 2020.

Vancouver:

Datta S. Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus. [Internet] [Doctoral dissertation]. Louisiana State University; 2009. [cited 2020 Feb 25]. Available from: etd-04142009-225600 ; https://digitalcommons.lsu.edu/gradschool_dissertations/29.

Council of Science Editors:

Datta S. Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus. [Doctoral Dissertation]. Louisiana State University; 2009. Available from: etd-04142009-225600 ; https://digitalcommons.lsu.edu/gradschool_dissertations/29

Tulane University

8. Yenni, Rachael. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.

Degree: 2016, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:75232

►

The humoral arm of the adaptive immune system involves the production of antibodies by cells of the B cell lineage, which bind and in some… (more)

Subjects/Keywords: Lassa antibodies epitopes

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yenni, R. (2016). Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:75232

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yenni, Rachael. “Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.” 2016. Thesis, Tulane University. Accessed February 25, 2020. https://digitallibrary.tulane.edu/islandora/object/tulane:75232.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yenni, Rachael. “Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.” 2016. Web. 25 Feb 2020.

Vancouver:

Yenni R. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. [Internet] [Thesis]. Tulane University; 2016. [cited 2020 Feb 25]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:75232.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yenni R. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. [Thesis]. Tulane University; 2016. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:75232

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

9. Moore, Penny L. Viral escape from HIV-1 neutralizing antibodies drives increased plasma neutralization breadth through sequential recognition of multiple epitopes and immunotypes.

Degree: 2013, University of KwaZulu-Natal

URL: http://hdl.handle.net/10413/10169

► Identifying the targets of broadly neutralizing antibodies to HIV-1 and understanding how these antibodies develop remain important goals in the quest to rationally develop an… (more)

Subjects/Keywords: Viral antigens.; Antibodies.; HIV infections – Immunology.; AIDS vaccines.; Neutralizing antibodies.; Viral antibodies.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moore, P. L. (2013). Viral escape from HIV-1 neutralizing antibodies drives increased plasma neutralization breadth through sequential recognition of multiple epitopes and immunotypes. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/10169

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moore, Penny L. “Viral escape from HIV-1 neutralizing antibodies drives increased plasma neutralization breadth through sequential recognition of multiple epitopes and immunotypes.” 2013. Thesis, University of KwaZulu-Natal. Accessed February 25, 2020. http://hdl.handle.net/10413/10169.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moore, Penny L. “Viral escape from HIV-1 neutralizing antibodies drives increased plasma neutralization breadth through sequential recognition of multiple epitopes and immunotypes.” 2013. Web. 25 Feb 2020.

Vancouver:

Moore PL. Viral escape from HIV-1 neutralizing antibodies drives increased plasma neutralization breadth through sequential recognition of multiple epitopes and immunotypes. [Internet] [Thesis]. University of KwaZulu-Natal; 2013. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/10413/10169.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moore PL. Viral escape from HIV-1 neutralizing antibodies drives increased plasma neutralization breadth through sequential recognition of multiple epitopes and immunotypes. [Thesis]. University of KwaZulu-Natal; 2013. Available from: http://hdl.handle.net/10413/10169

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

10. Peris, Daniela. In vitro diversification of chimeric human/chicken antibodies.

Degree: PhD, 2019, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/293408

► Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and refining affinities and specificities of existing ones (Cumbers et… (more)

Subjects/Keywords: Antibodies; DT40 cells; Antibody engineering; Chimeric antibodies; Antibody evolution; human antibodies; Antibody diversification

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peris, D. (2019). In vitro diversification of chimeric human/chicken antibodies. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/293408

Chicago Manual of Style (16^th Edition):

Peris, Daniela. “In vitro diversification of chimeric human/chicken antibodies.” 2019. Doctoral Dissertation, University of Cambridge. Accessed February 25, 2020. https://www.repository.cam.ac.uk/handle/1810/293408.

MLA Handbook (7^th Edition):

Peris, Daniela. “In vitro diversification of chimeric human/chicken antibodies.” 2019. Web. 25 Feb 2020.

Vancouver:

Peris D. In vitro diversification of chimeric human/chicken antibodies. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2020 Feb 25]. Available from: https://www.repository.cam.ac.uk/handle/1810/293408.

Council of Science Editors:

Peris D. In vitro diversification of chimeric human/chicken antibodies. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/293408

University of Dundee

11. Wong, Julin. Targeting c-Met for therapy.

Degree: PhD, 2011, University of Dundee

URL: http://hdl.handle.net/10588/a670d71d-758a-48b2-aa5b-2255b2373d7e

► c-Met is a tyrosine receptor kinase which is activated by its only ligand, the hepatocyte growth factor (HGF). Activation of c-Met leads to a wide… (more)

Subjects/Keywords: 616.994; c-Met; Antibodies; HGFR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, J. (2011). Targeting c-Met for therapy. (Doctoral Dissertation). University of Dundee. Retrieved from http://hdl.handle.net/10588/a670d71d-758a-48b2-aa5b-2255b2373d7e

Chicago Manual of Style (16^th Edition):

Wong, Julin. “Targeting c-Met for therapy.” 2011. Doctoral Dissertation, University of Dundee. Accessed February 25, 2020. http://hdl.handle.net/10588/a670d71d-758a-48b2-aa5b-2255b2373d7e.

MLA Handbook (7^th Edition):

Wong, Julin. “Targeting c-Met for therapy.” 2011. Web. 25 Feb 2020.

Vancouver:

Wong J. Targeting c-Met for therapy. [Internet] [Doctoral dissertation]. University of Dundee; 2011. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/10588/a670d71d-758a-48b2-aa5b-2255b2373d7e.

Council of Science Editors:

Wong J. Targeting c-Met for therapy. [Doctoral Dissertation]. University of Dundee; 2011. Available from: http://hdl.handle.net/10588/a670d71d-758a-48b2-aa5b-2255b2373d7e

University of Namibia

12. Hikufe, EH. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia .

Degree: 2016, University of Namibia

URL: http://hdl.handle.net/11070/1696

► Rabies kills over 55,000 people worldwide annually of which about 97% die resulting from the bite(s) of rabid dogs. Despite the free annual vaccination of… (more)

Subjects/Keywords: Rabies ; Dogs ; Antibodies ; Vaccination

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hikufe, E. (2016). Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia . (Thesis). University of Namibia. Retrieved from http://hdl.handle.net/11070/1696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hikufe, EH. “Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia .” 2016. Thesis, University of Namibia. Accessed February 25, 2020. http://hdl.handle.net/11070/1696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hikufe, EH. “Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia .” 2016. Web. 25 Feb 2020.

Vancouver:

Hikufe E. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia . [Internet] [Thesis]. University of Namibia; 2016. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/11070/1696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hikufe E. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia . [Thesis]. University of Namibia; 2016. Available from: http://hdl.handle.net/11070/1696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

13. Tolley, Neal Dean. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.

Degree: MS;, Pathology;, 1997, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

► Although the etiology of multiple sclerosis (MS) is unknown, several factors are believed to play a role in this disease: genetic components, immunologic elements, and… (more)

Subjects/Keywords: Immunology; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tolley, N. D. (1997). DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

Chicago Manual of Style (16^th Edition):

Tolley, Neal Dean. “DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.” 1997. Masters Thesis, University of Utah. Accessed February 25, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347.

MLA Handbook (7^th Edition):

Tolley, Neal Dean. “DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.” 1997. Web. 25 Feb 2020.

Vancouver:

Tolley ND. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. [Internet] [Masters thesis]. University of Utah; 1997. [cited 2020 Feb 25]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347.

Council of Science Editors:

Tolley ND. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. [Masters Thesis]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

University of Utah

14. Frost, Marisa Antónia. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.

Degree: PhD, Pathology;, 2005, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920

► Eosinophils play a dual role in the hose immune response. Whereas considerable evidence exists that are effective in defending the host against parasite infections, they… (more)

Subjects/Keywords: Proteins; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frost, M. A. (2005). Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920

Chicago Manual of Style (16^th Edition):

Frost, Marisa Antónia. “Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.” 2005. Doctoral Dissertation, University of Utah. Accessed February 25, 2020. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920.

MLA Handbook (7^th Edition):

Frost, Marisa Antónia. “Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.” 2005. Web. 25 Feb 2020.

Vancouver:

Frost MA. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. [Internet] [Doctoral dissertation]. University of Utah; 2005. [cited 2020 Feb 25]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920.

Council of Science Editors:

Frost MA. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. [Doctoral Dissertation]. University of Utah; 2005. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920

15. Ζάβαλη, Μαρία-Δημητρούλα. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.

Degree: 2009, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

►

Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε… (more)

▼

Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε αυτή την αναφορά, παρουσιάζεται μια μεθοδολογία η οποία βασίζεται στη Φασματοσκοπία Ανάκλασης Λευκού Φωτός και ενδείκνυται για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αντιδράσεων που λαμβάνουν χώρα σε στερεά υποστρώματα. Η οπτική διάταξη αποτελείται από μια VIS–NIR οπτική πηγή η οποία επικοινωνεί μέσω οπτικής ίνας με φασματοφωτόμετρο συνδεδεμένο σε Η/Υ. Το εξωτερικό τμήμα της οπτικής ίνας κατευθύνει το φως κάθετα πάνω στην επιφάνεια όπου συμβαίνουν οι αλληλεπιδράσεις μεταξύ των βιιομορίων. Το ανακλώμενο φως συγκεντρώνεται από το εσωτερικό τμήμα της οπτικής ίνας και κατευθύνεται στο φασματοφωτόμετρο. Οι αντιδράσεις λαμβάνουν χώρα σε επιφάνεια διοξειδίου του πυριτίου επικαλυμμένη με πολυμερικό υμένιο. Μια αντλία χρησιμοποιείται για την παροχή των αντιδραστηρίων με ελεγχόμενο ρυθμό σε ένα μικρορρευστομηχανικό κανάλι. Το φάσμα της ανάκλασης καταγράφεται σε πραγματικό χρόνο. Οι αντιδράσεις μεταξύ των πρωτεϊνικών μορίων γίνονται αντιληπτές σαν μετατοπίσεις του μήκους κύματος εκεί που παρατηρείται το φαινόμενο της ενισχυτικής συμβολής. Οι μετατοπίσεις αυτές στο μήκος κύματος συμβαίνουν λόγω του σχηματισμού πρωτεϊνικού στρώματος είτε κατά τη διάρκεια της προσρόφησης των αντισωμάτων στο στερεό υπόστρωμα ή λόγω αλλαγής στο πάχος του πρωτεϊνικού στρώματος όταν τα συμπληρωματικά αντιγόνα δεσμεύονται από τα ήδη ακινητοποιημένα αντισώματα. Η προτεινόμενη μεθοδολογία εφαρμόστηκε για την παρακολούθηση σε πραγματικό χρόνο, χωρίς τη χρήση ιχνηθέτη της αντίδρασης μεταξύ των συμπληρωματικών μορίων βιοτίνης-στρεπταβιδίνης όπως επίσης και για την ανίχνευση της πρόσδεσης των αντιγόνων mouse IgG από ήδη ακινητοποιημένα αντισώματα anti-mouse IgG. Mouse IgG σε συγκεντρώσεις μικρότερες των 150 pM ανιχνεύτηκαν σε πολύ μικρούς χρόνους αντίδρασης (10 min). Ο οπτικός αισθητήρας είναι μια απλή, γρήγορη, χαμηλού κόστους διάταξη για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων που τον καθιστά κατάλληλο για διάφορες αναλυτικές εφαρμογές.

Label-free monitoring of biomolecular reactions in real-time is of great interest since it can provide direct results concerning binding kinetics and equilibrium constants. In this report, a method based on White Light Reflectance Spectroscopy (WLRS) is presented that is capable for real-time monitoring of biomolecular reactions taking place on a solid surface. The optical setup consists of a VIS–NIR light source connected through a bifurcated optical fiber to a PC-driven spectrophotometer. The outer part of the optical fibre guides the light vertically onto the surface where the biomolecular reactions occur. The reflected light is collected from the central part of the optical fibre and is directed to the spectrophotometer. The reactions take place on the top of a polymer covered silicon dioxide surface. A microfluidic module in combination with a micropump is used to supply the…

Advisors/Committee Members: Κουτσούρης, Δημήτριος, Zavali, Maria-Dimitroula, Κουτσούρης, Δημήτριος, Νικήτα, Κωνσταντίνα, Μισιακός, Κωνστατίνος.

Subjects/Keywords: Βιοαισθητήρες; Αντισώματα; 610.28; Biosensors; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ζάβαλη, �. (2009). Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

Chicago Manual of Style (16^th Edition):

Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Masters Thesis, University of Patras. Accessed February 25, 2020. http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.

MLA Handbook (7^th Edition):

Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Web. 25 Feb 2020.

Vancouver:

Ζάβαλη �. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Internet] [Masters thesis]. University of Patras; 2009. [cited 2020 Feb 25]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.

Council of Science Editors:

Ζάβαλη �. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Masters Thesis]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

16. Khan, Asad. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.

Degree: Biochemistry, 2008, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/17961

►

Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive… (more)

▼

Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive species have been implicated in many disease conditions newlineincluding chronic inflammation, atherosclerosis, rheumatoid arthritis, some newlineneurodegenerative diseases and systemic lupus erythematosus. Systemic lupus newlineerythematosus and rheumatoid arthritis are autoimmune diseases with complex etiology newlineand pathogenesis. The abnormal level of nitrotyrosine detected in tissues affected by newlineabove diseases have been attributed to peroxynitrite mediated hypernitration of newlinetyrosine residues in proteins. Peroxynitrite is a potent oxidant as well as nitrating agent newlineand has in vivo existence. It is formed when nitric oxide reacts with superoxide radical. newlinePeroxynitrite is a powerful pro-inflammatory substance and may increase vascular newlinepermeability in inflamed tissues. newlineIn this doctoral thesis, physico-chemical and immunological studies have been newlinecarried out on histones modified by peroxynitrite with an objective of studying the newlinepossible role of oxidatively nitrated proteins in the initiation/progression of systemic newlinelupus erythematosus and rheumatoid arthritis. newlineAnalysis of UV absorption profile of peroxynitrite modified H1, H2A, H2B newlineand H3 histones revealed peak shift to higher wavelength and hyperchromicity at 276 newlinenm compared to native histones. Furthermore, in case of modified histones an newlineadditional peak was observed at 420 nm which corresponds to nitrotyrosine because a newlinestandard solution of 3-nitrotyrosine had given a similar peak under our experimental newlineconditions. The hyperchromicity observed in peroxynitrite modified histones samples newlinemight be due to nitration of tyrosine residues which ultimately enhances the molar newlineabsorptivity compared to native histones. HPLC analysis confirmed generation of newlinenitrotyrosine in peroxynitrite modified histones.

Bibliography p.142-160

Advisors/Committee Members: Alam, Khursheed.

Subjects/Keywords: Biotechnology; peroxynitrite; Antibodies; experimentally

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, A. (2008). Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Asad. “Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.” 2008. Thesis, Aligarh Muslim University. Accessed February 25, 2020. http://shodhganga.inflibnet.ac.in/handle/10603/17961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Asad. “Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.” 2008. Web. 25 Feb 2020.

Vancouver:

Khan A. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. [Internet] [Thesis]. Aligarh Muslim University; 2008. [cited 2020 Feb 25]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan A. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. [Thesis]. Aligarh Muslim University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

17. Sixholo, Joy. Engineering recombinant chicken antibodies for improved characteristics.

Degree: Veterinary Tropical Diseases, 2009, University of Pretoria

URL: http://hdl.handle.net/2263/30520

► Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can… (more)

▼ Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can be engineered to improve their binding or other characteristics. A chicken single chain variable fragment (scFv) phage library was panned against the 16 kDa antigen of Mycobacterium tuberculosis. Three phage displayed antibodies were obtained which bound specifically to the antigen. In soluble scFv format, however, they produced low ELISA signals. For this reason they were able to be used as models for antibody engineering. Three mutant sub-libraries were created by random mutagenesis. High stringency panning of the mutant sub-libraries against the target antigen yielded stronger binders which produced ELISA signals of up to eleven times higher than the parent scFvs. An increase in affinity was confirmed by surface plasmon resonance. One mutant scFv with a single amino acid exchange also showed an increase in the yield of scFvs it produced. Upon shortening the linker sequence between the heavy and light chains, size exclusion chromatography showed that multimerisation had occurred. Dimers, trimers and tetramers were formed thus increasing the avidity of the scFvs. Tetramers derived from the unmutated scFv showed the greatest improvement in ELISA binding. To improve expression and purification, an alternate bacterial expression vector with a histidine tag was investigated. For this series of experiments the coding region for a chicken scFv directed against VP7 of bluetongue virus was transferred from the pHEN1 display vector to the pSANG 14-3F vector, which fuses the scFv gene to a bacterial alkaline phosphatase gene. A bi-functional chicken scFv-alkaline phosphatase fusion protein that exhibits both alkaline phosphatase activity and specific antigen binding was expressed in Escherichia coli and purified in a single step via metal affinity chromatography. Furthermore the scFv-AP fusion protein was directly detected in ELISA without the use of secondary detection reagents. This study confirms that the strategies used can efficiently enhance the characteristics of chicken scFvs. Advisors/Committee Members: Dr J Fehrsen (advisor).

Subjects/Keywords: Chicken antibodies; Tuberculosis; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sixholo, J. (2009). Engineering recombinant chicken antibodies for improved characteristics. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/30520

Chicago Manual of Style (16^th Edition):

Sixholo, Joy. “Engineering recombinant chicken antibodies for improved characteristics.” 2009. Masters Thesis, University of Pretoria. Accessed February 25, 2020. http://hdl.handle.net/2263/30520.

MLA Handbook (7^th Edition):

Sixholo, Joy. “Engineering recombinant chicken antibodies for improved characteristics.” 2009. Web. 25 Feb 2020.

Vancouver:

Sixholo J. Engineering recombinant chicken antibodies for improved characteristics. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2263/30520.

Council of Science Editors:

Sixholo J. Engineering recombinant chicken antibodies for improved characteristics. [Masters Thesis]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/30520

University of Pretoria

18. Van der Merwe, Hermanus Daniel. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome.

Degree: MSc, Biochemistry, 2008, University of Pretoria

URL: http://hdl.handle.net/2263/23995

► Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and… (more)

▼ Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and is fatal in five percent of cases. Antibodies specific to several nerve components are frequently associated with clinical symptoms in GBS. These antibodies were found to be specific to various gangliosides and ganglioside complexes. It was also found that antibody reactivity to gangliosides is affected by membrane components. The most prevalent (20-30%) immunoglobulin in GBS is anti-GM1 (20-30%), which also binds to the LPS of the PEN O:19 Campylobacter jejuni serotype. This is the most common infectious agent associated with GBS and emphasizes the importance of infection and anti-ganglioside antibodies in disease development. Intravenous infusion of pooled immunoglobulin from healthy donors, also called intravenous immunoglobulin (IVIg), halves the severity of disease manifestation. The action mechanism of IVIg in curing GBS is not clear, but intravenous immunoglobulin was shown to neutralize anti-ganglioside binding activity and its pathogenic effects. It was further found that anti-idiotypic antibodies in IVIg inhibit anti-ganglioside antibody activity. Treatment with IVIg is not equally effective in all GBS cases, which might be due to the inability of IVIg to neutralize anti-ganglioside antibodies in all patients adequately. Therefore, the treatment of GBS with IVIg needs to be better understood in order to improve its use as a cure for GBS. This study confirmed previous findings that the interaction of patient serum anti-GM1 antibodies and ganglioside auto-antigens is greatly impaired by components in healthy serum. Bound anti-GM1 antibodies could be displaced by (presumably) anti-idiotypic antibodies from healthy donor serum. This study found that the displacement potential between donor sera differs. Anti-GM1 antibody displacement was found to be dependent on the character of both anti-GM1 and anti-idiotypic antibody. This demonstrated the feasibility of improving the efficiency of treatment by IVIg by sourcing it from only those sera that test best for displacing auto-antibodies from their ganglioside antigens in ELISA. IVIg selection may therefore greatly benefit from the use of recombinant phage display antibodies to distinguish between the various types of GBS for treatment. To develop a method to characterize anti-ganglioside antibodies sensitively, an evanescent field biosensor was employed in which gangliosides were presented in a liposome environment. This provided a more physiological way of antibody antigen recognition. The optimized method determined the ganglioside binding specificity of purified IgG from a GBS patient, and mouse monoclonal anti-GM1 and anti-GD1a antibodies accurately. The results compared well with those from ELISA. The results obtained with purified IgG were far better than that obtained with whole serum analysis. This could be due to non-specific binding or the presence of inhibiting anti-idiotypic antibodies in… Advisors/Committee Members: Prof J A Verschoor (advisor).

Subjects/Keywords: Antibody-antigen interactions; Antibodies; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van der Merwe, H. (2008). A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/23995

Chicago Manual of Style (16^th Edition):

Van der Merwe, Hermanus. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome.” 2008. Masters Thesis, University of Pretoria. Accessed February 25, 2020. http://hdl.handle.net/2263/23995.

MLA Handbook (7^th Edition):

Van der Merwe, Hermanus. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome.” 2008. Web. 25 Feb 2020.

Vancouver:

Van der Merwe H. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2263/23995.

Council of Science Editors:

Van der Merwe H. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/23995

University of Pretoria

19. [No author]. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome .

Degree: 2008, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

► Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and… (more)

▼ Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and is fatal in five percent of cases. Antibodies specific to several nerve components are frequently associated with clinical symptoms in GBS. These antibodies were found to be specific to various gangliosides and ganglioside complexes. It was also found that antibody reactivity to gangliosides is affected by membrane components. The most prevalent (20-30%) immunoglobulin in GBS is anti-GM1 (20-30%), which also binds to the LPS of the PEN O:19 Campylobacter jejuni serotype. This is the most common infectious agent associated with GBS and emphasizes the importance of infection and anti-ganglioside antibodies in disease development. Intravenous infusion of pooled immunoglobulin from healthy donors, also called intravenous immunoglobulin (IVIg), halves the severity of disease manifestation. The action mechanism of IVIg in curing GBS is not clear, but intravenous immunoglobulin was shown to neutralize anti-ganglioside binding activity and its pathogenic effects. It was further found that anti-idiotypic antibodies in IVIg inhibit anti-ganglioside antibody activity. Treatment with IVIg is not equally effective in all GBS cases, which might be due to the inability of IVIg to neutralize anti-ganglioside antibodies in all patients adequately. Therefore, the treatment of GBS with IVIg needs to be better understood in order to improve its use as a cure for GBS. This study confirmed previous findings that the interaction of patient serum anti-GM1 antibodies and ganglioside auto-antigens is greatly impaired by components in healthy serum. Bound anti-GM1 antibodies could be displaced by (presumably) anti-idiotypic antibodies from healthy donor serum. This study found that the displacement potential between donor sera differs. Anti-GM1 antibody displacement was found to be dependent on the character of both anti-GM1 and anti-idiotypic antibody. This demonstrated the feasibility of improving the efficiency of treatment by IVIg by sourcing it from only those sera that test best for displacing auto-antibodies from their ganglioside antigens in ELISA. IVIg selection may therefore greatly benefit from the use of recombinant phage display antibodies to distinguish between the various types of GBS for treatment. To develop a method to characterize anti-ganglioside antibodies sensitively, an evanescent field biosensor was employed in which gangliosides were presented in a liposome environment. This provided a more physiological way of antibody antigen recognition. The optimized method determined the ganglioside binding specificity of purified IgG from a GBS patient, and mouse monoclonal anti-GM1 and anti-GD1a antibodies accurately. The results compared well with those from ELISA. The results obtained with purified IgG were far better than that obtained with whole serum analysis. This could be due to non-specific binding or the presence of inhibiting anti-idiotypic antibodies in… Advisors/Committee Members: Prof J A Verschoor (advisor).

Subjects/Keywords: Antibody-antigen interactions; Antibodies; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2008). A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

Chicago Manual of Style (16^th Edition):

author], [No. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome .” 2008. Masters Thesis, University of Pretoria. Accessed February 25, 2020. http://upetd.up.ac.za/thesis/available/etd-04172008-111655/.

MLA Handbook (7^th Edition):

author], [No. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome .” 2008. Web. 25 Feb 2020.

Vancouver:

author] [. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome . [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2020 Feb 25]. Available from: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/.

Council of Science Editors:

author] [. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome . [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

University of Pretoria

20. [No author]. Engineering recombinant chicken antibodies for improved characteristics .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

► Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can… (more)

▼ Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can be engineered to improve their binding or other characteristics. A chicken single chain variable fragment (scFv) phage library was panned against the 16 kDa antigen of Mycobacterium tuberculosis. Three phage displayed antibodies were obtained which bound specifically to the antigen. In soluble scFv format, however, they produced low ELISA signals. For this reason they were able to be used as models for antibody engineering. Three mutant sub-libraries were created by random mutagenesis. High stringency panning of the mutant sub-libraries against the target antigen yielded stronger binders which produced ELISA signals of up to eleven times higher than the parent scFvs. An increase in affinity was confirmed by surface plasmon resonance. One mutant scFv with a single amino acid exchange also showed an increase in the yield of scFvs it produced. Upon shortening the linker sequence between the heavy and light chains, size exclusion chromatography showed that multimerisation had occurred. Dimers, trimers and tetramers were formed thus increasing the avidity of the scFvs. Tetramers derived from the unmutated scFv showed the greatest improvement in ELISA binding. To improve expression and purification, an alternate bacterial expression vector with a histidine tag was investigated. For this series of experiments the coding region for a chicken scFv directed against VP7 of bluetongue virus was transferred from the pHEN1 display vector to the pSANG 14-3F vector, which fuses the scFv gene to a bacterial alkaline phosphatase gene. A bi-functional chicken scFv-alkaline phosphatase fusion protein that exhibits both alkaline phosphatase activity and specific antigen binding was expressed in Escherichia coli and purified in a single step via metal affinity chromatography. Furthermore the scFv-AP fusion protein was directly detected in ELISA without the use of secondary detection reagents. This study confirms that the strategies used can efficiently enhance the characteristics of chicken scFvs. Advisors/Committee Members: Dr J Fehrsen (advisor).

Subjects/Keywords: Chicken antibodies; Tuberculosis; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2009). Engineering recombinant chicken antibodies for improved characteristics . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

Chicago Manual of Style (16^th Edition):

author], [No. “Engineering recombinant chicken antibodies for improved characteristics .” 2009. Masters Thesis, University of Pretoria. Accessed February 25, 2020. http://upetd.up.ac.za/thesis/available/etd-02202009-170916/.

MLA Handbook (7^th Edition):

author], [No. “Engineering recombinant chicken antibodies for improved characteristics .” 2009. Web. 25 Feb 2020.

Vancouver:

author] [. Engineering recombinant chicken antibodies for improved characteristics . [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2020 Feb 25]. Available from: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/.

Council of Science Editors:

author] [. Engineering recombinant chicken antibodies for improved characteristics . [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

Universiteit Utrecht

21. Liang, K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.

Degree: 2010, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/188023

► Biosimilars, defined as biological medicinal products comparable in quality, safety and efficacy to reference products, follow the independent regulatory pathway in the EU for marketing… (more)

Subjects/Keywords: Biosimilar; monoclonal antibodies (mAbs)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liang, K. (2010). Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/188023

Chicago Manual of Style (16^th Edition):

Liang, K. “Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.” 2010. Masters Thesis, Universiteit Utrecht. Accessed February 25, 2020. http://dspace.library.uu.nl:8080/handle/1874/188023.

MLA Handbook (7^th Edition):

Liang, K. “Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.” 2010. Web. 25 Feb 2020.

Vancouver:

Liang K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. [Internet] [Masters thesis]. Universiteit Utrecht; 2010. [cited 2020 Feb 25]. Available from: http://dspace.library.uu.nl:8080/handle/1874/188023.

Council of Science Editors:

Liang K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. [Masters Thesis]. Universiteit Utrecht; 2010. Available from: http://dspace.library.uu.nl:8080/handle/1874/188023

University of Aberdeen

22. Cupit, Pauline M. Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris.

Degree: PhD, 1996, University of Aberdeen

URL: http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078319 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694

► A recombinant single chain antibody (scAb) fragment specific for the isoenzyme human type V acid phosphatase has been expressed and secreted from Escherichia coli at… (more)

Subjects/Keywords: 572.8; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cupit, P. M. (1996). Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078319 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694

Chicago Manual of Style (16^th Edition):

Cupit, Pauline M. “Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris.” 1996. Doctoral Dissertation, University of Aberdeen. Accessed February 25, 2020. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078319 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694.

MLA Handbook (7^th Edition):

Cupit, Pauline M. “Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris.” 1996. Web. 25 Feb 2020.

Vancouver:

Cupit PM. Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris. [Internet] [Doctoral dissertation]. University of Aberdeen; 1996. [cited 2020 Feb 25]. Available from: http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078319 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694.

Council of Science Editors:

Cupit PM. Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris. [Doctoral Dissertation]. University of Aberdeen; 1996. Available from: http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078319 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694

Louisiana State University

23. Jadeja, Ravirajsinh. Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus.

Degree: PhD, Life Sciences, 2010, Louisiana State University

URL: etd-11192010-085050 ; https://digitalcommons.lsu.edu/gradschool_dissertations/529

► In the US there are about 76 million foodborne illness cases reported every year, in spite of initiatives by federal agencies. This emphasizes the need… (more)

Subjects/Keywords: Immunoassay; Vibrio vulnificus; Monoclonal Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jadeja, R. (2010). Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-11192010-085050 ; https://digitalcommons.lsu.edu/gradschool_dissertations/529

Chicago Manual of Style (16^th Edition):

Jadeja, Ravirajsinh. “Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus.” 2010. Doctoral Dissertation, Louisiana State University. Accessed February 25, 2020. etd-11192010-085050 ; https://digitalcommons.lsu.edu/gradschool_dissertations/529.

MLA Handbook (7^th Edition):

Jadeja, Ravirajsinh. “Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus.” 2010. Web. 25 Feb 2020.

Vancouver:

Jadeja R. Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus. [Internet] [Doctoral dissertation]. Louisiana State University; 2010. [cited 2020 Feb 25]. Available from: etd-11192010-085050 ; https://digitalcommons.lsu.edu/gradschool_dissertations/529.

Council of Science Editors:

Jadeja R. Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus. [Doctoral Dissertation]. Louisiana State University; 2010. Available from: etd-11192010-085050 ; https://digitalcommons.lsu.edu/gradschool_dissertations/529

University of Sydney

24. Irving, Harry George. The presence of renal autoantibodies in multiple sclerosis .

Degree: 2016, University of Sydney

URL: http://hdl.handle.net/2123/16518

► Multiple sclerosis (MS) is a chronic neurological disorder characterised by demyelinating inflammatory lesions within the central nervous system, which are separated in both time and… (more)

▼ Multiple sclerosis (MS) is a chronic neurological disorder characterised by demyelinating inflammatory lesions within the central nervous system, which are separated in both time and space. Although the cause of MS is unknown it is generally agreed that the disease is a result of an autoimmune response against components of the central nervous system (CNS) that occurs through complex interactions between genetic and environmental factors. There is growing evidence that autoreactive antibodies may play a role in the progression of the disease analogous to the role that has been established for aquaporin-‐4 antibodies in the pathogenic process of the demyelinating disease, neuromyelitis optica (NMO). In the process of screening the sera of neurological patients for NMO antibodies, a preliminary study found that a novel renal antibody may be present in patients with MS. This renal antibody bound to elements in the proximal brush border and was referred to as ABBA (anti-‐brush border antibody). The current study aimed to: determine the prevalence of ABBA in MS, determine whether there is a correlation with ABBA and any brain antibodies and determine the target to which ABBA binds. Indirect immunofluorescence on rat renal and neural tissue was used to determine the prevalence of renal antibodies while human renal cell lines were used to determine the antigenic target to which these antibodies bind. This study is the first to investigate the presence of ABBA and other renal antibodies in MS patients and the first to identify high titre antibodies in the disease. While the presence of ABBA was not found to be significantly different between MS cases and control groups, ABBA was found to be more prevalent in inflammatory neurological conditions and a correlation was found between ABBA and axonal-‐nodal antibodies in inflammatory conditions. The current study was unable to identify the target to which ABBA binds. The presence of high titre antiglomerular antibodies (AGLA), anti-‐distal tubule (ADA) and anti-‐cortical collecting duct antibodies (ACCA) in the serum of patients with MS was an unexpected and novel finding and has not previously been documented. Neuroinflammatory cases had a higher prevalence of AGLA, which were also associated with the presence of both anti-‐axonal nodal antibodies and anti-‐ perivascular antibodies. In the absence of renal pathology or documented nephrotoxic medications as well as not identifying the antigenic target, the significance of this observation remains unclear. However, possible explanations are discussed.

Subjects/Keywords: Multiple Sclerosis; autoantibodies; renal antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Irving, H. G. (2016). The presence of renal autoantibodies in multiple sclerosis . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/16518

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Irving, Harry George. “The presence of renal autoantibodies in multiple sclerosis .” 2016. Thesis, University of Sydney. Accessed February 25, 2020. http://hdl.handle.net/2123/16518.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Irving, Harry George. “The presence of renal autoantibodies in multiple sclerosis .” 2016. Web. 25 Feb 2020.

Vancouver:

Irving HG. The presence of renal autoantibodies in multiple sclerosis . [Internet] [Thesis]. University of Sydney; 2016. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2123/16518.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Irving HG. The presence of renal autoantibodies in multiple sclerosis . [Thesis]. University of Sydney; 2016. Available from: http://hdl.handle.net/2123/16518

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

25. Bhanot, Anisha. Maternal antibodies in autism: what is known and future directions.

Degree: MS, Medical Sciences, 2018, Boston University

URL: http://hdl.handle.net/2144/30896

► Autism spectrum disorder (ASD) refers to a highly prevalent neuropsychiatric disorder, currently affecting one in every 68 children. ASD is understood as a heterogeneous disorder,… (more)

Subjects/Keywords: Neurosciences; Antibodies; Autism; Immunology; Neurodevelopment

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bhanot, A. (2018). Maternal antibodies in autism: what is known and future directions. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/30896

Chicago Manual of Style (16^th Edition):

Bhanot, Anisha. “Maternal antibodies in autism: what is known and future directions.” 2018. Masters Thesis, Boston University. Accessed February 25, 2020. http://hdl.handle.net/2144/30896.

MLA Handbook (7^th Edition):

Bhanot, Anisha. “Maternal antibodies in autism: what is known and future directions.” 2018. Web. 25 Feb 2020.

Vancouver:

Bhanot A. Maternal antibodies in autism: what is known and future directions. [Internet] [Masters thesis]. Boston University; 2018. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/2144/30896.

Council of Science Editors:

Bhanot A. Maternal antibodies in autism: what is known and future directions. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/30896

University of Dundee

26. Wong, Julin. Targeting c-Met for therapy.

Degree: PhD, 2011, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820

► c-Met is a tyrosine receptor kinase which is activated by its only ligand, the hepatocyte growth factor (HGF). Activation of c-Met leads to a wide… (more)

Subjects/Keywords: 616.994; c-Met; Antibodies; HGFR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, J. (2011). Targeting c-Met for therapy. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820

Chicago Manual of Style (16^th Edition):

Wong, Julin. “Targeting c-Met for therapy.” 2011. Doctoral Dissertation, University of Dundee. Accessed February 25, 2020. https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820.

MLA Handbook (7^th Edition):

Wong, Julin. “Targeting c-Met for therapy.” 2011. Web. 25 Feb 2020.

Vancouver:

Wong J. Targeting c-Met for therapy. [Internet] [Doctoral dissertation]. University of Dundee; 2011. [cited 2020 Feb 25]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820.

Council of Science Editors:

Wong J. Targeting c-Met for therapy. [Doctoral Dissertation]. University of Dundee; 2011. Available from: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820

University of California – Santa Cruz

27. Fedechkin, Stanislav. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.

Degree: Chemistry, 2018, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/21c5j93j

► Respiratory syncytial virus (RSV) is a top cause of severe lower respiratory tract disease and mortality in young children and the elderly. The viral envelope… (more)

Subjects/Keywords: Biochemistry; Antibodies; Attachment; G; RSV

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fedechkin, S. (2018). Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/21c5j93j

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fedechkin, Stanislav. “Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.” 2018. Thesis, University of California – Santa Cruz. Accessed February 25, 2020. http://www.escholarship.org/uc/item/21c5j93j.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fedechkin, Stanislav. “Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.” 2018. Web. 25 Feb 2020.

Vancouver:

Fedechkin S. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. [Internet] [Thesis]. University of California – Santa Cruz; 2018. [cited 2020 Feb 25]. Available from: http://www.escholarship.org/uc/item/21c5j93j.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fedechkin S. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. [Thesis]. University of California – Santa Cruz; 2018. Available from: http://www.escholarship.org/uc/item/21c5j93j

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. YAP PENG KANG. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.

Degree: 1996, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/153065

Subjects/Keywords: Monoclonal antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

KANG, Y. P. (1996). STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/153065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

KANG, YAP PENG. “STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.” 1996. Thesis, National University of Singapore. Accessed February 25, 2020. https://scholarbank.nus.edu.sg/handle/10635/153065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

KANG, YAP PENG. “STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.” 1996. Web. 25 Feb 2020.

Vancouver:

KANG YP. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. [Internet] [Thesis]. National University of Singapore; 1996. [cited 2020 Feb 25]. Available from: https://scholarbank.nus.edu.sg/handle/10635/153065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

KANG YP. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. [Thesis]. National University of Singapore; 1996. Available from: https://scholarbank.nus.edu.sg/handle/10635/153065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

29. Lau, Esther. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/70019

►

Receptor tyrosine kinases or RTKs are an important class of signaling proteins that are frequently deregulated in cancer and other diseases. Based on a series… (more)

Subjects/Keywords: HER3; PTK7; synthetic antibodies; cancer

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lau, E. (2013). Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70019

Chicago Manual of Style (16^th Edition):

Lau, Esther. “Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.” 2013. Masters Thesis, University of Toronto. Accessed February 25, 2020. http://hdl.handle.net/1807/70019.

MLA Handbook (7^th Edition):

Lau, Esther. “Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.” 2013. Web. 25 Feb 2020.

Vancouver:

Lau E. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2020 Feb 25]. Available from: http://hdl.handle.net/1807/70019.

Council of Science Editors:

Lau E. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/70019

University of New South Wales

30. Swift, Joseph. Identification of small molecule inhibitors of protein aggregation.

Degree: Biotechnology & Biomolecular Sciences, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/52779 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11452/SOURCE01?view=true

► Therapeutic antibodies utilize the adaptive immune response to treat complex disease states. Antibodies’ success in therapeutic applications has led to their domination of total biologics… (more)

▼ Therapeutic antibodies utilize the adaptive immune response to treat complex disease states. Antibodies’ success in therapeutic applications has led to their domination of total biologics revenue. With more therapeutic antibodies currently in development, as well as many new drug targets being discovered, there is little doubt that this sector will continue to grow. However, bottlenecks exist in therapeutic antibody development. One constraint in antibody development is antibody aggregation. Formulating antibodies can be problematic, as in vitro preparations require high protein concentrations that can promote aggregation. Each antibody peptide sequence is unique within complimentary determining regions (CDRs); this region provides antibody specificity to an antigen target. However the CDR region can also determine the aggregation properties of the molecule. The resulting difference in aggregation properties between antibodies prevents development of a general approach to inhibit aggregation occurring in vitro. Antibody aggregation can also cause disease. Light Chain Deposition Disease (LCDD) is a condition caused by aggregation of light chain antibodies in vivo. It often results in amyloid deposits affecting vital organ function. The primary aim of this thesis was to discover a chemical compound that could inhibit in vitro antibody aggregation. A tertiary aim was to establish that a candidate compound could also serve as a potential drug candidate in LCDD treatment. To achieve this, CDR mediated aggregation was modeled with variable light chain domain antibodies (VLs) displayed on phage and in soluble form. A method utilizing phage-display was developed to detect FDA approved compounds that could inhibit CDR mediated VL aggregation. Specifically, The Prestwick Chemical Library of 1200 compounds was screened with phage expressing aggregation prone VL DPK9 using a ‘heat-cool’ ELISA method. This screen identified Levodopa and Apomorphine as compounds that could prevent aggregation of DPK9. Further testing demonstrated that Levodopa and Apomorphine could also successfully prevent aggregation of soluble VL proteins. Structural analysis demonstrated that the catechol moiety present on both Levodopa and Apomorphine prevented aggregation of VL protein. This led to the discovery of four other polyphenol compounds also capable of preventing VL aggregation. Further experimentation also allowed for the mechanism of action of the catechol moiety to be partially understood. All candidate compounds identified were either FDA approved or naturally occurring polyphenols. Consequently such compounds may have direct implications as excipients in antibody in vitro formulation and as drug candidates for treatment of LCDD. Advisors/Committee Members: Christ, Daniel , Clinical School - St Vincent's Hospital, Faculty of Medicine, UNSW.

Subjects/Keywords: Aggregation; Domain antibodies; Polyphenols

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Swift, J. (2013). Identification of small molecule inhibitors of protein aggregation. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52779 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11452/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Swift, Joseph. “Identification of small molecule inhibitors of protein aggregation.” 2013. Masters Thesis, University of New South Wales. Accessed February 25, 2020. http://handle.unsw.edu.au/1959.4/52779 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11452/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Swift, Joseph. “Identification of small molecule inhibitors of protein aggregation.” 2013. Web. 25 Feb 2020.

Vancouver:

Swift J. Identification of small molecule inhibitors of protein aggregation. [Internet] [Masters thesis]. University of New South Wales; 2013. [cited 2020 Feb 25]. Available from: http://handle.unsw.edu.au/1959.4/52779 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11452/SOURCE01?view=true.

Council of Science Editors:

Swift J. Identification of small molecule inhibitors of protein aggregation. [Masters Thesis]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/52779 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11452/SOURCE01?view=true

Open Access Theses and Dissertations