subject:(Antibodies)

University of Texas – Austin

1. Carroll, Sean Matthew. Strategies for generating therapeutic antibodies.

Degree: PhD, Chemical Engineering, 2012, University of Texas – Austin

URL: http://hdl.handle.net/2152/ETD-UT-2012-08-5990

► Monoclonal antibodies have become essential therapeutic tools and currently dominate the therapeutic protein market. Consequently, there is continued demand for new therapeutic antibodies and their… (more)

▼ Monoclonal antibodies have become essential therapeutic tools and currently dominate the therapeutic protein market. Consequently, there is continued demand for new therapeutic antibodies and their discovery techniques. In one part of this work, we report the discovery of a new therapeutic antibody candidate with a novel mechanism for inhibition of a therapeutically relevant biochemical pathway: the classical complement pathway. In order to inhibit classical complement, an antibody was developed that modulates the signaling subcomponent of the pathway initiating C1 complex, C1s. This work includes novel protocols and strategies used for discovery and characterization of antibody D, which binds and inhibits C1s protease activity. By regulating C1s activity, antibody D is shown to regulate classical complement. It is further shown that affinity maturation of antibody D results in higher levels of complement inhibition at various antibody concentrations. This work marks the first example of an antibody that specifically regulates the classical complement pathway by targeting the C1s protease on the pathway initiating C1-complex. Next, we characterize the human immune cells produced by humanized NSG mice, most notably the B and T lymphocytes, engraftment with human CD34+ HSC cells. We detected development of naïve human B and T cells and their various subtypes, as well as other human immune cells from engrafted mice. However, attempts to generate a robust antibody response to antigens were unsuccessful. Therefore, we conclude that NSG humanized mice developed in this study are suitable for studying the antibody repertoire of naïve B cells, however they are not suitable for the analysis of activated B-cells. Last, we introduce a novel strategy for the generation of polarized antibody repertoires for use in therapeutic monoclonal antibody discovery. This technique combines targeted antigen delivery to a specific lymph node and a frequency based antibody selection approach in order to directly select antigen specific antibodies in silico. By directly selecting antigen-specific antibodies, this approach circumvents laborious and time consuming screening techniques. We expect that this work will be the foundation of an overall improved protocol for monoclonal antibody discovery that accelerates the speed and enhances the simplicity of discovery techniques. Advisors/Committee Members: Iverson, Brent L. (advisor), Georgiou, George (advisor), Alper, Hal (committee member), Maynard, Jennifer (committee member), Tucker, Philip (committee member).

Subjects/Keywords: Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carroll, S. M. (2012). Strategies for generating therapeutic antibodies. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2012-08-5990

Chicago Manual of Style (16^th Edition):

Carroll, Sean Matthew. “Strategies for generating therapeutic antibodies.” 2012. Doctoral Dissertation, University of Texas – Austin. Accessed March 02, 2021. http://hdl.handle.net/2152/ETD-UT-2012-08-5990.

MLA Handbook (7^th Edition):

Carroll, Sean Matthew. “Strategies for generating therapeutic antibodies.” 2012. Web. 02 Mar 2021.

Vancouver:

Carroll SM. Strategies for generating therapeutic antibodies. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2152/ETD-UT-2012-08-5990.

Council of Science Editors:

Carroll SM. Strategies for generating therapeutic antibodies. [Doctoral Dissertation]. University of Texas – Austin; 2012. Available from: http://hdl.handle.net/2152/ETD-UT-2012-08-5990

Vanderbilt University

2. Briney, Bryan Scott. The development and genetic origin of broadly neutralizing HIV antibodies.

Degree: PhD, Microbiology and Immunology, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/13651

► Several of the most broadly neutralizing HIV antibodies (bnAbs) contain unique genetic or structural elements, including long heavy chain complementarity determining region 3 (HCDR3) loops… (more)

▼ Several of the most broadly neutralizing HIV antibodies (bnAbs) contain unique genetic or structural elements, including long heavy chain complementarity determining region 3 (HCDR3) loops and extensive somatic hypermutation. Two exceptionally broad, potently neutralizing HIV-specific antibodies, PG9 and PG16, encode HCDR3 loops that are among the longest of any antigen-specific antibody described to date. Passive immunization with two other bnAbs that encode long HCDR3s, 4E10 and 2F5, is able to protect against HIV infection. Induction of such long HCDR3 antibodies may be critical to the design of an effective vaccine strategy for HIV, however it is unclear at present how to induce such antibodies. There has been speculation that antibodies with long HCDR3s are generated primarily through the accumulation of somatic hypermutation-associated insertions. These short insertion events are rare, and design of an immunogen that efficiently induces many such insertions in a single antibody sequence is likely to be extremely difficult. Through the use of high-throughput antibody sequencing, I have identified genetic evidence that these long HCDR3 antibodies are typically formed at the original recombination event, not through accumulation of somatic hypermutation-induced insertions. Antibodies with long HCDR3s were found in all tested individuals, and long HCDR3 antibodies typically use a restricted subset of D and J gene segments, resulting in the incorporation of highly conserved genetic elements in the majority of such antibody sequences. This work provides an important first step toward the realization of a vaccine that efficiently induces broadly neutralizing HIV antibodies with long HCDR3s by identifying a conserved genetic target through which B cells encoding antibodies with long HCDR3s may be induced selectively through vaccination. Advisors/Committee Members: Billy G. Hudson (committee member), Christopher R. Aiken (committee member), Spyros A. Kalams (committee member), James E. Crowe, Jr. (committee member), James W. Thomas (Committee Chair).

Subjects/Keywords: antibodies; HIV

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Briney, B. S. (2012). The development and genetic origin of broadly neutralizing HIV antibodies. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13651

Chicago Manual of Style (16^th Edition):

Briney, Bryan Scott. “The development and genetic origin of broadly neutralizing HIV antibodies.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 02, 2021. http://hdl.handle.net/1803/13651.

MLA Handbook (7^th Edition):

Briney, Bryan Scott. “The development and genetic origin of broadly neutralizing HIV antibodies.” 2012. Web. 02 Mar 2021.

Vancouver:

Briney BS. The development and genetic origin of broadly neutralizing HIV antibodies. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1803/13651.

Council of Science Editors:

Briney BS. The development and genetic origin of broadly neutralizing HIV antibodies. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/13651

Boston University

3. Russell, Anthony. Antibodies involved in homograft rejection.

Degree: MA, Biology, 1960, Boston University

URL: http://hdl.handle.net/2144/24569

Subjects/Keywords: Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Russell, A. (1960). Antibodies involved in homograft rejection. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/24569

Chicago Manual of Style (16^th Edition):

Russell, Anthony. “Antibodies involved in homograft rejection.” 1960. Masters Thesis, Boston University. Accessed March 02, 2021. http://hdl.handle.net/2144/24569.

MLA Handbook (7^th Edition):

Russell, Anthony. “Antibodies involved in homograft rejection.” 1960. Web. 02 Mar 2021.

Vancouver:

Russell A. Antibodies involved in homograft rejection. [Internet] [Masters thesis]. Boston University; 1960. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2144/24569.

Council of Science Editors:

Russell A. Antibodies involved in homograft rejection. [Masters Thesis]. Boston University; 1960. Available from: http://hdl.handle.net/2144/24569

McGill University

4. Pan, Bin-Tao. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.

Degree: PhD, Department of Biochemistry, 1982, McGill University

URL: https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866

►

Des antigènes spécifiques de la surface membranaire de réticulocytes de mouton ont été isoles en utilisant des anticorps spécifiquement développés contre la surface cellulaire. Le… (more)

Subjects/Keywords: Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pan, B. (1982). Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866

Chicago Manual of Style (16^th Edition):

Pan, Bin-Tao. “Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.” 1982. Doctoral Dissertation, McGill University. Accessed March 02, 2021. https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866.

MLA Handbook (7^th Edition):

Pan, Bin-Tao. “Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.” 1982. Web. 02 Mar 2021.

Vancouver:

Pan B. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. [Internet] [Doctoral dissertation]. McGill University; 1982. [cited 2021 Mar 02]. Available from: https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866.

Council of Science Editors:

Pan B. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. [Doctoral Dissertation]. McGill University; 1982. Available from: https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866

University of New South Wales

5. Mi, Yang. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.

Degree: Graduate School of Biomedical Engineering, 2014, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

► Angiogenesis relies on the coordination of endothelial cells, smooth muscle cells and the vascular extracellular matrix around cells. Perlecan is an important heparan sulfate proteoglycan,… (more)

▼ Angiogenesis relies on the coordination of endothelial cells, smooth muscle cells and the vascular extracellular matrix around cells. Perlecan is an important heparan sulfate proteoglycan, which is widely expressed in vascular tissues and essentially involved in angiogenesis. Perlecan exhibits pro- and anti-angiogenic effects differently according to the cell line from which it was derived from and various structures especially its dependent glycosaminoglycans. The aim of the thesis was to generate and characterise monoclonal antibodies against specific domains of perlecan, and investigate their functions in modulating angiogenesis.lmmunopurified human endothelial cell-derived perlecan decorated with heparin sulfate and recombinant perlecan domain V decorated with chondroitin sulfate were characterised by Enzyme linked immunosorbent assay, immunocytochemistry and Western blotting, and then used as antigens for hybridoma screening. There were 205 single clones generated by limiting dilution, while 174 clones were able to recognise endothelial perlecan. 48 clones with high viability and lg production were tested with intact perlecan and Hep Ill treated perlecan, while they showed different immunoreactive changes between two antigens. However, none of the clones reacted with recombinant perlecan domain I or domain V.Of the newly generated monoclonal antibodies (mAbs), 5 mAbs showed potential to modulate the adhesion of endothelial cells and smooth muscle cells, especially 8C8 and 4F2 reduced the adhesion of both cell types. In addition, the 5 mAbs displayed strong immunoreactivity with perlecan derived from HCAECs and HVSMCs in Western blotting and were able to detect the perlecan expressed on two types of cells in immunocytochemistry. In spreading assay, all 5 mAbs were found to inhibit the HVSMC and HCAEC spreading to different degrees. Moreover, the 5 mAbs except 5G8 showed inhibitory effects in HCAEC migration, while only 8C8, 4F7 and 4F2 were able to reduce the migration of HVSMCs. These 5 mAbs could be useful to investigate the presence and structure of perlecan as primary antibodies, since they were able to recognise the perlecan protein core. They could also become potential tools to explain the functions of different domains of perlecan in angiogenesis and developed as immunotherapeutic drugs to mediate angiogenesis in clinical treatments.

Subjects/Keywords: Antibodies; Perlecan

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mi, Y. (2014). Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Mi, Yang. “Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.” 2014. Masters Thesis, University of New South Wales. Accessed March 02, 2021. http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Mi, Yang. “Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.” 2014. Web. 02 Mar 2021.

Vancouver:

Mi Y. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. [Internet] [Masters thesis]. University of New South Wales; 2014. [cited 2021 Mar 02]. Available from: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true.

Council of Science Editors:

Mi Y. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. [Masters Thesis]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

Rutgers University

6. Prabhu, Siddharth, 1995-. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.

Degree: MS, Chemical and Biochemical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

► Monoclonal antibodies are the fastest growing segment of pharmaceutical molecules. Currently, they are used as diagnostics, therapeutics for various medical uses as well as in… (more)

Subjects/Keywords: Monoclonal antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prabhu, Siddharth, 1. (2018). Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

Chicago Manual of Style (16^th Edition):

Prabhu, Siddharth, 1995-. “Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.” 2018. Masters Thesis, Rutgers University. Accessed March 02, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/59136/.

MLA Handbook (7^th Edition):

Prabhu, Siddharth, 1995-. “Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.” 2018. Web. 02 Mar 2021.

Vancouver:

Prabhu, Siddharth 1. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. [Internet] [Masters thesis]. Rutgers University; 2018. [cited 2021 Mar 02]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/.

Council of Science Editors:

Prabhu, Siddharth 1. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. [Masters Thesis]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

University of Utah

7. Peterson, Lisa K. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.

Degree: PhD, Pathology;, 2007, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

► Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is an autoimmune model of MS, induced by… (more)

▼ Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is an autoimmune model of MS, induced by sensitization with myelin proteins of their encephalitogenic peptides. Sensitization with amino acids 92-106 of myelin oligodendrocyte glycoprotein (MOG) induces different disease courses and neuropathology in A.SW and SJL/J mice. SJL/J mice develop a relapsing-remitting (RR-EAE) disease course characterized by mild demyelinating disease with perivascular T cell infiltration. In contrast, A.SW mice develop a progressive (P-EAE) disease course characterized by large areas of plaque-like demyelination with Ig deposition, polymorphonuclear (PMN) cell infiltration and minimal T cell infiltration and high MOG antibody titers in the serum. This thesis describes, as part of the elucidation of the role of antibody in determining disease course and neuropathology, the generation and characterization of hybridomas producing antibody reactive with MOG92-106. Two MOG92-106 hybridomas produced polyreactive IgM antibodies encoded by immunoglobulin genes in their germline configuration, indicating that the antibodies are natural autoantibodies (NAA). These MOG92-106 reactive NNA bind myelin and cause demyelination in vivo, suggesting that they could contribute to the pathogenesis of P-EAE. In addition, sensitization of A.S.W. mice with MOG92-106 or intraperitoneal injection of the MOG92-106 reactive hybridomas resulted in antibody deposition in glomeruli of kidneys and proteinuria, demonstrating that MOG92-106 reacative NAA can induce systemic autoimmunity. However, depletion of B-1 cells, the main produces of NAA, had minimal effects on the development of demyelination, antibody deposition or disease progression in mice sensitized with MOG92-106. These results indicate that while MOG92-106 reacative NAA are capable of inducing pathology in the CNS and kidneys, they play a minor role in the pathogenesis of MOG92-106-infduced EAE. In addition, this thesis describes induction of another combination of disease course and neuropathology in B10.S mice. B10.S mice have previously been considered resistant to EAE, but sensitization with MOG92-106 resulted in neuropathology in 93% of the mice and monophasic or relapsing-remitting disease course in 30% of the mice. Taken together, this work expands the spectrum of autoimmune disease that can be induced and investigated using MOG92-106.

Subjects/Keywords: Multiple Sclerosis; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peterson, L. K. (2007). Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

Chicago Manual of Style (16^th Edition):

Peterson, Lisa K. “Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.” 2007. Doctoral Dissertation, University of Utah. Accessed March 02, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453.

MLA Handbook (7^th Edition):

Peterson, Lisa K. “Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.” 2007. Web. 02 Mar 2021.

Vancouver:

Peterson LK. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. [Internet] [Doctoral dissertation]. University of Utah; 2007. [cited 2021 Mar 02]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453.

Council of Science Editors:

Peterson LK. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. [Doctoral Dissertation]. University of Utah; 2007. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

Tulane University

8. Yenni, Rachael. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.

Degree: 2016, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:75232

►

The humoral arm of the adaptive immune system involves the production of antibodies by cells of the B cell lineage, which bind and in some… (more)

Subjects/Keywords: Lassa antibodies epitopes

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yenni, R. (2016). Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:75232

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yenni, Rachael. “Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.” 2016. Thesis, Tulane University. Accessed March 02, 2021. https://digitallibrary.tulane.edu/islandora/object/tulane:75232.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yenni, Rachael. “Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.” 2016. Web. 02 Mar 2021.

Vancouver:

Yenni R. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. [Internet] [Thesis]. Tulane University; 2016. [cited 2021 Mar 02]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:75232.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yenni R. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. [Thesis]. Tulane University; 2016. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:75232

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida Atlantic University

9. Cavallo, Michelle Fay. ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS.

Degree: 2019, Florida Atlantic University

URL: http://fau.digital.flvc.org/islandora/object/fau:42161

►

Two novel methodologies were developed for purification and functional (DNA hydrolytic) assessment of anti-DNA antibodies of IgG isotype from patients with Systemic Lupus Erythematosus (SLE).… (more)

Subjects/Keywords: Systemic lupus erythematosus; Anti-DNA antibodies; DNA antibodies; Antibodies, Catalytic; Autoantibodies – Analysis; Antibodies – isolation & purification

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cavallo, M. F. (2019). ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS. (Thesis). Florida Atlantic University. Retrieved from http://fau.digital.flvc.org/islandora/object/fau:42161

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cavallo, Michelle Fay. “ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS.” 2019. Thesis, Florida Atlantic University. Accessed March 02, 2021. http://fau.digital.flvc.org/islandora/object/fau:42161.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cavallo, Michelle Fay. “ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS.” 2019. Web. 02 Mar 2021.

Vancouver:

Cavallo MF. ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS. [Internet] [Thesis]. Florida Atlantic University; 2019. [cited 2021 Mar 02]. Available from: http://fau.digital.flvc.org/islandora/object/fau:42161.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cavallo MF. ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS. [Thesis]. Florida Atlantic University; 2019. Available from: http://fau.digital.flvc.org/islandora/object/fau:42161

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

10. Peris, Daniela. In vitro diversification of chimeric human/chicken antibodies.

Degree: PhD, 2019, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/293408

► Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and refining affinities and specificities of existing ones (Cumbers et… (more)

Subjects/Keywords: Antibodies; DT40 cells; Antibody engineering; Chimeric antibodies; Antibody evolution; human antibodies; Antibody diversification

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peris, D. (2019). In vitro diversification of chimeric human/chicken antibodies. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/293408

Chicago Manual of Style (16^th Edition):

Peris, Daniela. “In vitro diversification of chimeric human/chicken antibodies.” 2019. Doctoral Dissertation, University of Cambridge. Accessed March 02, 2021. https://www.repository.cam.ac.uk/handle/1810/293408.

MLA Handbook (7^th Edition):

Peris, Daniela. “In vitro diversification of chimeric human/chicken antibodies.” 2019. Web. 02 Mar 2021.

Vancouver:

Peris D. In vitro diversification of chimeric human/chicken antibodies. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Mar 02]. Available from: https://www.repository.cam.ac.uk/handle/1810/293408.

Council of Science Editors:

Peris D. In vitro diversification of chimeric human/chicken antibodies. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/293408

University of Pretoria

11. [No author]. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome .

Degree: 2008, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

► Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and… (more)

▼ Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and is fatal in five percent of cases. Antibodies specific to several nerve components are frequently associated with clinical symptoms in GBS. These antibodies were found to be specific to various gangliosides and ganglioside complexes. It was also found that antibody reactivity to gangliosides is affected by membrane components. The most prevalent (20-30%) immunoglobulin in GBS is anti-GM1 (20-30%), which also binds to the LPS of the PEN O:19 Campylobacter jejuni serotype. This is the most common infectious agent associated with GBS and emphasizes the importance of infection and anti-ganglioside antibodies in disease development. Intravenous infusion of pooled immunoglobulin from healthy donors, also called intravenous immunoglobulin (IVIg), halves the severity of disease manifestation. The action mechanism of IVIg in curing GBS is not clear, but intravenous immunoglobulin was shown to neutralize anti-ganglioside binding activity and its pathogenic effects. It was further found that anti-idiotypic antibodies in IVIg inhibit anti-ganglioside antibody activity. Treatment with IVIg is not equally effective in all GBS cases, which might be due to the inability of IVIg to neutralize anti-ganglioside antibodies in all patients adequately. Therefore, the treatment of GBS with IVIg needs to be better understood in order to improve its use as a cure for GBS. This study confirmed previous findings that the interaction of patient serum anti-GM1 antibodies and ganglioside auto-antigens is greatly impaired by components in healthy serum. Bound anti-GM1 antibodies could be displaced by (presumably) anti-idiotypic antibodies from healthy donor serum. This study found that the displacement potential between donor sera differs. Anti-GM1 antibody displacement was found to be dependent on the character of both anti-GM1 and anti-idiotypic antibody. This demonstrated the feasibility of improving the efficiency of treatment by IVIg by sourcing it from only those sera that test best for displacing auto-antibodies from their ganglioside antigens in ELISA. IVIg selection may therefore greatly benefit from the use of recombinant phage display antibodies to distinguish between the various types of GBS for treatment. To develop a method to characterize anti-ganglioside antibodies sensitively, an evanescent field biosensor was employed in which gangliosides were presented in a liposome environment. This provided a more physiological way of antibody antigen recognition. The optimized method determined the ganglioside binding specificity of purified IgG from a GBS patient, and mouse monoclonal anti-GM1 and anti-GD1a antibodies accurately. The results compared well with those from ELISA. The results obtained with purified IgG were far better than that obtained with whole serum analysis. This could be due to non-specific binding or the presence of inhibiting anti-idiotypic antibodies in… Advisors/Committee Members: Prof J A Verschoor (advisor).

Subjects/Keywords: Antibody-antigen interactions; Antibodies; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2008). A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

Chicago Manual of Style (16^th Edition):

author], [No. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome .” 2008. Masters Thesis, University of Pretoria. Accessed March 02, 2021. http://upetd.up.ac.za/thesis/available/etd-04172008-111655/.

MLA Handbook (7^th Edition):

author], [No. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome .” 2008. Web. 02 Mar 2021.

Vancouver:

author] [. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome . [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 02]. Available from: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/.

Council of Science Editors:

author] [. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome . [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

University of Pretoria

12. [No author]. Engineering recombinant chicken antibodies for improved characteristics .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

► Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can… (more)

▼ Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can be engineered to improve their binding or other characteristics. A chicken single chain variable fragment (scFv) phage library was panned against the 16 kDa antigen of Mycobacterium tuberculosis. Three phage displayed antibodies were obtained which bound specifically to the antigen. In soluble scFv format, however, they produced low ELISA signals. For this reason they were able to be used as models for antibody engineering. Three mutant sub-libraries were created by random mutagenesis. High stringency panning of the mutant sub-libraries against the target antigen yielded stronger binders which produced ELISA signals of up to eleven times higher than the parent scFvs. An increase in affinity was confirmed by surface plasmon resonance. One mutant scFv with a single amino acid exchange also showed an increase in the yield of scFvs it produced. Upon shortening the linker sequence between the heavy and light chains, size exclusion chromatography showed that multimerisation had occurred. Dimers, trimers and tetramers were formed thus increasing the avidity of the scFvs. Tetramers derived from the unmutated scFv showed the greatest improvement in ELISA binding. To improve expression and purification, an alternate bacterial expression vector with a histidine tag was investigated. For this series of experiments the coding region for a chicken scFv directed against VP7 of bluetongue virus was transferred from the pHEN1 display vector to the pSANG 14-3F vector, which fuses the scFv gene to a bacterial alkaline phosphatase gene. A bi-functional chicken scFv-alkaline phosphatase fusion protein that exhibits both alkaline phosphatase activity and specific antigen binding was expressed in Escherichia coli and purified in a single step via metal affinity chromatography. Furthermore the scFv-AP fusion protein was directly detected in ELISA without the use of secondary detection reagents. This study confirms that the strategies used can efficiently enhance the characteristics of chicken scFvs. Advisors/Committee Members: Dr J Fehrsen (advisor).

Subjects/Keywords: Chicken antibodies; Tuberculosis; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2009). Engineering recombinant chicken antibodies for improved characteristics . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

Chicago Manual of Style (16^th Edition):

author], [No. “Engineering recombinant chicken antibodies for improved characteristics .” 2009. Masters Thesis, University of Pretoria. Accessed March 02, 2021. http://upetd.up.ac.za/thesis/available/etd-02202009-170916/.

MLA Handbook (7^th Edition):

author], [No. “Engineering recombinant chicken antibodies for improved characteristics .” 2009. Web. 02 Mar 2021.

Vancouver:

author] [. Engineering recombinant chicken antibodies for improved characteristics . [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Mar 02]. Available from: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/.

Council of Science Editors:

author] [. Engineering recombinant chicken antibodies for improved characteristics . [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

Universiteit Utrecht

13. Liang, K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.

Degree: 2010, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/188023

► Biosimilars, defined as biological medicinal products comparable in quality, safety and efficacy to reference products, follow the independent regulatory pathway in the EU for marketing… (more)

Subjects/Keywords: Biosimilar; monoclonal antibodies (mAbs)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liang, K. (2010). Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/188023

Chicago Manual of Style (16^th Edition):

Liang, K. “Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.” 2010. Masters Thesis, Universiteit Utrecht. Accessed March 02, 2021. http://dspace.library.uu.nl:8080/handle/1874/188023.

MLA Handbook (7^th Edition):

Liang, K. “Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.” 2010. Web. 02 Mar 2021.

Vancouver:

Liang K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. [Internet] [Masters thesis]. Universiteit Utrecht; 2010. [cited 2021 Mar 02]. Available from: http://dspace.library.uu.nl:8080/handle/1874/188023.

Council of Science Editors:

Liang K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. [Masters Thesis]. Universiteit Utrecht; 2010. Available from: http://dspace.library.uu.nl:8080/handle/1874/188023

University of Namibia

14. Hikufe, EH. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia .

Degree: 2016, University of Namibia

URL: http://hdl.handle.net/11070/1696

► Rabies kills over 55,000 people worldwide annually of which about 97% die resulting from the bite(s) of rabid dogs. Despite the free annual vaccination of… (more)

Subjects/Keywords: Rabies ; Dogs ; Antibodies ; Vaccination

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hikufe, E. (2016). Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia . (Thesis). University of Namibia. Retrieved from http://hdl.handle.net/11070/1696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hikufe, EH. “Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia .” 2016. Thesis, University of Namibia. Accessed March 02, 2021. http://hdl.handle.net/11070/1696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hikufe, EH. “Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia .” 2016. Web. 02 Mar 2021.

Vancouver:

Hikufe E. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia . [Internet] [Thesis]. University of Namibia; 2016. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/11070/1696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hikufe E. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia . [Thesis]. University of Namibia; 2016. Available from: http://hdl.handle.net/11070/1696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

15. Tolley, Neal Dean. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.

Degree: MS;, Pathology;, 1997, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

► Although the etiology of multiple sclerosis (MS) is unknown, several factors are believed to play a role in this disease: genetic components, immunologic elements, and… (more)

Subjects/Keywords: Immunology; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tolley, N. D. (1997). DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

Chicago Manual of Style (16^th Edition):

Tolley, Neal Dean. “DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.” 1997. Masters Thesis, University of Utah. Accessed March 02, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347.

MLA Handbook (7^th Edition):

Tolley, Neal Dean. “DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.” 1997. Web. 02 Mar 2021.

Vancouver:

Tolley ND. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. [Internet] [Masters thesis]. University of Utah; 1997. [cited 2021 Mar 02]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347.

Council of Science Editors:

Tolley ND. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. [Masters Thesis]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

University of Utah

16. Frost, Marisa Antónia. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.

Degree: PhD, Pathology;, 2005, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920

► Eosinophils play a dual role in the hose immune response. Whereas considerable evidence exists that are effective in defending the host against parasite infections, they… (more)

Subjects/Keywords: Proteins; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frost, M. A. (2005). Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920

Chicago Manual of Style (16^th Edition):

Frost, Marisa Antónia. “Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.” 2005. Doctoral Dissertation, University of Utah. Accessed March 02, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920.

MLA Handbook (7^th Edition):

Frost, Marisa Antónia. “Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.” 2005. Web. 02 Mar 2021.

Vancouver:

Frost MA. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. [Internet] [Doctoral dissertation]. University of Utah; 2005. [cited 2021 Mar 02]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920.

Council of Science Editors:

Frost MA. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. [Doctoral Dissertation]. University of Utah; 2005. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920

17. Ζάβαλη, Μαρία-Δημητρούλα. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.

Degree: 2009, University of Patras

URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

►

Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε… (more)

▼

Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε αυτή την αναφορά, παρουσιάζεται μια μεθοδολογία η οποία βασίζεται στη Φασματοσκοπία Ανάκλασης Λευκού Φωτός και ενδείκνυται για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αντιδράσεων που λαμβάνουν χώρα σε στερεά υποστρώματα. Η οπτική διάταξη αποτελείται από μια VIS–NIR οπτική πηγή η οποία επικοινωνεί μέσω οπτικής ίνας με φασματοφωτόμετρο συνδεδεμένο σε Η/Υ. Το εξωτερικό τμήμα της οπτικής ίνας κατευθύνει το φως κάθετα πάνω στην επιφάνεια όπου συμβαίνουν οι αλληλεπιδράσεις μεταξύ των βιιομορίων. Το ανακλώμενο φως συγκεντρώνεται από το εσωτερικό τμήμα της οπτικής ίνας και κατευθύνεται στο φασματοφωτόμετρο. Οι αντιδράσεις λαμβάνουν χώρα σε επιφάνεια διοξειδίου του πυριτίου επικαλυμμένη με πολυμερικό υμένιο. Μια αντλία χρησιμοποιείται για την παροχή των αντιδραστηρίων με ελεγχόμενο ρυθμό σε ένα μικρορρευστομηχανικό κανάλι. Το φάσμα της ανάκλασης καταγράφεται σε πραγματικό χρόνο. Οι αντιδράσεις μεταξύ των πρωτεϊνικών μορίων γίνονται αντιληπτές σαν μετατοπίσεις του μήκους κύματος εκεί που παρατηρείται το φαινόμενο της ενισχυτικής συμβολής. Οι μετατοπίσεις αυτές στο μήκος κύματος συμβαίνουν λόγω του σχηματισμού πρωτεϊνικού στρώματος είτε κατά τη διάρκεια της προσρόφησης των αντισωμάτων στο στερεό υπόστρωμα ή λόγω αλλαγής στο πάχος του πρωτεϊνικού στρώματος όταν τα συμπληρωματικά αντιγόνα δεσμεύονται από τα ήδη ακινητοποιημένα αντισώματα. Η προτεινόμενη μεθοδολογία εφαρμόστηκε για την παρακολούθηση σε πραγματικό χρόνο, χωρίς τη χρήση ιχνηθέτη της αντίδρασης μεταξύ των συμπληρωματικών μορίων βιοτίνης-στρεπταβιδίνης όπως επίσης και για την ανίχνευση της πρόσδεσης των αντιγόνων mouse IgG από ήδη ακινητοποιημένα αντισώματα anti-mouse IgG. Mouse IgG σε συγκεντρώσεις μικρότερες των 150 pM ανιχνεύτηκαν σε πολύ μικρούς χρόνους αντίδρασης (10 min). Ο οπτικός αισθητήρας είναι μια απλή, γρήγορη, χαμηλού κόστους διάταξη για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων που τον καθιστά κατάλληλο για διάφορες αναλυτικές εφαρμογές.

Label-free monitoring of biomolecular reactions in real-time is of great interest since it can provide direct results concerning binding kinetics and equilibrium constants. In this report, a method based on White Light Reflectance Spectroscopy (WLRS) is presented that is capable for real-time monitoring of biomolecular reactions taking place on a solid surface. The optical setup consists of a VIS–NIR light source connected through a bifurcated optical fiber to a PC-driven spectrophotometer. The outer part of the optical fibre guides the light vertically onto the surface where the biomolecular reactions occur. The reflected light is collected from the central part of the optical fibre and is directed to the spectrophotometer. The reactions take place on the top of a polymer covered silicon dioxide surface. A microfluidic module in combination with a micropump is used to supply the…

Advisors/Committee Members: Κουτσούρης, Δημήτριος, Zavali, Maria-Dimitroula, Κουτσούρης, Δημήτριος, Νικήτα, Κωνσταντίνα, Μισιακός, Κωνστατίνος.

Subjects/Keywords: Βιοαισθητήρες; Αντισώματα; 610.28; Biosensors; Antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ζάβαλη, �. (2009). Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

Chicago Manual of Style (16^th Edition):

Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Masters Thesis, University of Patras. Accessed March 02, 2021. http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.

MLA Handbook (7^th Edition):

Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Web. 02 Mar 2021.

Vancouver:

Ζάβαλη �. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Internet] [Masters thesis]. University of Patras; 2009. [cited 2021 Mar 02]. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.

Council of Science Editors:

Ζάβαλη �. Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Masters Thesis]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124

18. Khan, Asad. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.

Degree: Biochemistry, 2008, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/17961

►

Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive… (more)

▼

Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive species have been implicated in many disease conditions newlineincluding chronic inflammation, atherosclerosis, rheumatoid arthritis, some newlineneurodegenerative diseases and systemic lupus erythematosus. Systemic lupus newlineerythematosus and rheumatoid arthritis are autoimmune diseases with complex etiology newlineand pathogenesis. The abnormal level of nitrotyrosine detected in tissues affected by newlineabove diseases have been attributed to peroxynitrite mediated hypernitration of newlinetyrosine residues in proteins. Peroxynitrite is a potent oxidant as well as nitrating agent newlineand has in vivo existence. It is formed when nitric oxide reacts with superoxide radical. newlinePeroxynitrite is a powerful pro-inflammatory substance and may increase vascular newlinepermeability in inflamed tissues. newlineIn this doctoral thesis, physico-chemical and immunological studies have been newlinecarried out on histones modified by peroxynitrite with an objective of studying the newlinepossible role of oxidatively nitrated proteins in the initiation/progression of systemic newlinelupus erythematosus and rheumatoid arthritis. newlineAnalysis of UV absorption profile of peroxynitrite modified H1, H2A, H2B newlineand H3 histones revealed peak shift to higher wavelength and hyperchromicity at 276 newlinenm compared to native histones. Furthermore, in case of modified histones an newlineadditional peak was observed at 420 nm which corresponds to nitrotyrosine because a newlinestandard solution of 3-nitrotyrosine had given a similar peak under our experimental newlineconditions. The hyperchromicity observed in peroxynitrite modified histones samples newlinemight be due to nitration of tyrosine residues which ultimately enhances the molar newlineabsorptivity compared to native histones. HPLC analysis confirmed generation of newlinenitrotyrosine in peroxynitrite modified histones.

Bibliography p.142-160

Advisors/Committee Members: Alam, Khursheed.

Subjects/Keywords: Biotechnology; peroxynitrite; Antibodies; experimentally

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, A. (2008). Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Asad. “Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.” 2008. Thesis, Aligarh Muslim University. Accessed March 02, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/17961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Asad. “Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -.” 2008. Web. 02 Mar 2021.

Vancouver:

Khan A. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. [Internet] [Thesis]. Aligarh Muslim University; 2008. [cited 2021 Mar 02]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan A. Studies on experimentally produced Antibodies against peroxynitrite Modified histones; -. [Thesis]. Aligarh Muslim University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

19. Van der Merwe, Hermanus Daniel. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome.

Degree: MSc, Biochemistry, 2008, University of Pretoria

URL: http://hdl.handle.net/2263/23995

► Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and… (more)

▼ Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and is fatal in five percent of cases. Antibodies specific to several nerve components are frequently associated with clinical symptoms in GBS. These antibodies were found to be specific to various gangliosides and ganglioside complexes. It was also found that antibody reactivity to gangliosides is affected by membrane components. The most prevalent (20-30%) immunoglobulin in GBS is anti-GM1 (20-30%), which also binds to the LPS of the PEN O:19 Campylobacter jejuni serotype. This is the most common infectious agent associated with GBS and emphasizes the importance of infection and anti-ganglioside antibodies in disease development. Intravenous infusion of pooled immunoglobulin from healthy donors, also called intravenous immunoglobulin (IVIg), halves the severity of disease manifestation. The action mechanism of IVIg in curing GBS is not clear, but intravenous immunoglobulin was shown to neutralize anti-ganglioside binding activity and its pathogenic effects. It was further found that anti-idiotypic antibodies in IVIg inhibit anti-ganglioside antibody activity. Treatment with IVIg is not equally effective in all GBS cases, which might be due to the inability of IVIg to neutralize anti-ganglioside antibodies in all patients adequately. Therefore, the treatment of GBS with IVIg needs to be better understood in order to improve its use as a cure for GBS. This study confirmed previous findings that the interaction of patient serum anti-GM1 antibodies and ganglioside auto-antigens is greatly impaired by components in healthy serum. Bound anti-GM1 antibodies could be displaced by (presumably) anti-idiotypic antibodies from healthy donor serum. This study found that the displacement potential between donor sera differs. Anti-GM1 antibody displacement was found to be dependent on the character of both anti-GM1 and anti-idiotypic antibody. This demonstrated the feasibility of improving the efficiency of treatment by IVIg by sourcing it from only those sera that test best for displacing auto-antibodies from their ganglioside antigens in ELISA. IVIg selection may therefore greatly benefit from the use of recombinant phage display antibodies to distinguish between the various types of GBS for treatment. To develop a method to characterize anti-ganglioside antibodies sensitively, an evanescent field biosensor was employed in which gangliosides were presented in a liposome environment. This provided a more physiological way of antibody antigen recognition. The optimized method determined the ganglioside binding specificity of purified IgG from a GBS patient, and mouse monoclonal anti-GM1 and anti-GD1a antibodies accurately. The results compared well with those from ELISA. The results obtained with purified IgG were far better than that obtained with whole serum analysis. This could be due to non-specific binding or the presence of inhibiting anti-idiotypic antibodies in… Advisors/Committee Members: Prof J A Verschoor (advisor).

Subjects/Keywords: Antibody-antigen interactions; Antibodies; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van der Merwe, H. (2008). A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/23995

Chicago Manual of Style (16^th Edition):

Van der Merwe, Hermanus. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome.” 2008. Masters Thesis, University of Pretoria. Accessed March 02, 2021. http://hdl.handle.net/2263/23995.

MLA Handbook (7^th Edition):

Van der Merwe, Hermanus. “A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome.” 2008. Web. 02 Mar 2021.

Vancouver:

Van der Merwe H. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2263/23995.

Council of Science Editors:

Van der Merwe H. A resonant mirror biosensor approach to understand antibody-antigen interactions in Guillain Barré Syndrome. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/23995

University of Toronto

20. Lau, Esther. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/70019

►

Receptor tyrosine kinases or RTKs are an important class of signaling proteins that are frequently deregulated in cancer and other diseases. Based on a series… (more)

Subjects/Keywords: HER3; PTK7; synthetic antibodies; cancer

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lau, E. (2013). Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70019

Chicago Manual of Style (16^th Edition):

Lau, Esther. “Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.” 2013. Masters Thesis, University of Toronto. Accessed March 02, 2021. http://hdl.handle.net/1807/70019.

MLA Handbook (7^th Edition):

Lau, Esther. “Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.” 2013. Web. 02 Mar 2021.

Vancouver:

Lau E. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/1807/70019.

Council of Science Editors:

Lau E. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/70019

University of Wisconsin – La Cross

21. Jaedike, Alicia. Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection.

Degree: 2019, University of Wisconsin – La Cross

URL: http://digital.library.wisc.edu/1793/80314

► Influenza is a highly contagious viral pathogen that causes respiratory illness in humans and animals. It is a major public health threat, resulting in three… (more)

Subjects/Keywords: Monoclonal antibodies; Influenza; Ferrets

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jaedike, A. (2019). Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection. (Thesis). University of Wisconsin – La Cross. Retrieved from http://digital.library.wisc.edu/1793/80314

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jaedike, Alicia. “Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection.” 2019. Thesis, University of Wisconsin – La Cross. Accessed March 02, 2021. http://digital.library.wisc.edu/1793/80314.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jaedike, Alicia. “Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection.” 2019. Web. 02 Mar 2021.

Vancouver:

Jaedike A. Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection. [Internet] [Thesis]. University of Wisconsin – La Cross; 2019. [cited 2021 Mar 02]. Available from: http://digital.library.wisc.edu/1793/80314.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jaedike A. Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection. [Thesis]. University of Wisconsin – La Cross; 2019. Available from: http://digital.library.wisc.edu/1793/80314

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

22. Desmond, Angela. New Approaches to the Development of Peptoid Vaccines.

Degree: 2015, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1573

► The ideal prophylactic vaccine against a toxin or pathogen should elicit the production of broadly protective antibodies against conserved epitopes. However, the epitopes that elicit… (more)

▼ The ideal prophylactic vaccine against a toxin or pathogen should elicit the production of broadly protective antibodies against conserved epitopes. However, the epitopes that elicit these antibodies are often not immunodominant and even when they are, characterizing and synthesizing them can be difficult, particularly if they are conformational. The long-term goal of this work was to develop prophylactic vaccines that elicit such antibodies without epitope characterization. To develop such a vaccine platform, it was hypothesized that screening large one-bead-one-compound libraries of synthetic compounds with monoclonal antibodies that have already been shown to be broadly protective against a toxin or pathogen would allow the identification of mimetic B cell epitopes. For this platform, peptoids were chosen to construct one-bead-one-compound libraries. Peptoids are N-oligosubstituted glycines that resemble peptides but bear their side groups on backbone nitrogens instead of carbons. This renders them protease resistant and enormously diverse, since they are not restricted to the twenty standard amino acids. Furthermore, previous work had demonstrated that a monoclonal antibody could be used to screen libraries of peptoids. Moreover, while peptoids themselves were not immunogenic, the attachment of peptoids to carrier proteins using a linker elicited antibodies against the peptoid/linker. Such T-cell dependent antigens elicited high-affinity, class-switched antibodies. The goal of this dissertation research was to continue optimizing the magnetic and color-based assays by which peptoid vaccine candidates could be identified and to screen libraries with neutralizing monoclonal antibodies against West Nile virus and murine norovirus type 1. In addition, the immunogenicity of peptoids was further examined by designing a peptoid-carrier, using it to immunize rabbits, and demonstrating that anti-peptoid antibodies could be affinity-purified from the resulting antisera. This antibody was then used in further optimization of the magnetic screening assay to ensure that future screens will efficiently and specifically identify the best vaccine candidates. Advisors/Committee Members: Pfeiffer, Julie K., Vitetta, Ellen S., Levine, Beth, Niederkorn, Jerry Y., Ward, E. Sally.

Subjects/Keywords: Antibodies, Monoclonal; Peptoids; Vaccines

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Desmond, A. (2015). New Approaches to the Development of Peptoid Vaccines. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Desmond, Angela. “New Approaches to the Development of Peptoid Vaccines.” 2015. Thesis, University of Texas Southwestern Medical Center. Accessed March 02, 2021. http://hdl.handle.net/2152.5/1573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Desmond, Angela. “New Approaches to the Development of Peptoid Vaccines.” 2015. Web. 02 Mar 2021.

Vancouver:

Desmond A. New Approaches to the Development of Peptoid Vaccines. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2015. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2152.5/1573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Desmond A. New Approaches to the Development of Peptoid Vaccines. [Thesis]. University of Texas Southwestern Medical Center; 2015. Available from: http://hdl.handle.net/2152.5/1573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

23. Velmurugan, Ramraj. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.

Degree: 2017, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/7195

►

The file named "VELMURUGAN-DISSERTATION-2017.pdf" is the primary dissertation file. Eight (8) supplemental files are also available and may be viewed individually.

The use of monoclonal… (more)

▼

The file named "VELMURUGAN-DISSERTATION-2017.pdf" is the primary dissertation file. Eight (8) supplemental files are also available and may be viewed individually.

The use of monoclonal antibodies represents a rapidly expanding area for cancer therapy. One of the main mechanisms of action of these antibodies is Fcγ receptor-mediated engagement of macrophages and other immune cells. When macrophages engage tumor cells opsonized with antibody molecules, they can perform trogocytosis, the process of internalizing fragments of the target cell, or phagocytosis, the internalization of entire cancer cells. This study first establishes whether the process of trogocytosis can lead to cancer cell death. A variety of microscopy and flow cytometric assays were used to quantify the levels of trogocytosis and cell death, in co-cultures of macrophages and cancer cells. Using HER2-overexpressing breast cancer cell lines and anti-HER2 antibodies, we show that persistent trogocytosis can lead to the killing of cancer cells. The mechanism of trogocytosis was also explored using multifocal plane microscopy (MUM). Imaging the process of trogocytosis using MUM revealed that it proceeds through the macrophage-mediated extrusion of tubular structures of the target cell membrane. This membrane-tubulation results in the preferential uptake of the membrane components from the target cell. The study also investigated the maturation pathway followed by phagosomes containing entire cancer cells. A vacuole-like structure associates with these phagosomes, which whilst also lysosomal in nature, displays characteristics distinct from the phagosome itself. The interface between the vacuole and the phagosome is impermeable to certain solutes as observed through microscopy. Further, the size of the phagosome-associated vacuole is affected by inhibition of the mTOR pathway. Use of advanced microscopy techniques such as MUM in these and other biological problems provides mechanistic insight at the spatiotemporal level. To further develop the algorithms involved in MUM data processing, I have therefore also explored various non-parametric methods of estimating the axial location of point sources from MUM data. A new non-parametric method is proposed, which uses multiple intensities calculated from each image of a point source in MUM data. The performance of this approach is compared with other non-parametric methods through simulations and Fisher information calculations. The effectiveness of this method on experimental data is also evaluated.

Advisors/Committee Members: Li, Wen-Hong, Ward, E. Sally, Ober, Raimund J., Alexandrakis, Georgios, Pasare, Chandrashekhar.

Subjects/Keywords: Antibodies; Breast Neoplasms; Macrophages; Phagocytosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Velmurugan, R. (2017). Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/7195

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Velmurugan, Ramraj. “Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.” 2017. Thesis, University of Texas Southwestern Medical Center. Accessed March 02, 2021. http://hdl.handle.net/2152.5/7195.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Velmurugan, Ramraj. “Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.” 2017. Web. 02 Mar 2021.

Vancouver:

Velmurugan R. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2017. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2152.5/7195.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Velmurugan R. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. [Thesis]. University of Texas Southwestern Medical Center; 2017. Available from: http://hdl.handle.net/2152.5/7195

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

24. Sullivan, Laura Anne. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.

Degree: 2012, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1040

► Angiogenesis is the development of blood vessels from a pre-existing vascular network. This process is essential during growth, development and wound healing and plays a… (more)

▼ Angiogenesis is the development of blood vessels from a pre-existing vascular network. This process is essential during growth, development and wound healing and plays a critical role in the growth and progression of cancer. Initial tumor size is restricted by the diffusion capacity of oxygen and nutrients from surrounding blood vessels. Therefore, to progress beyond a volume of several millimeters, a tumor must stimulate angiogenesis to generate a vascular network that will supply the tumor with the necessary blood, oxygen and nutrients that will allow for continued growth, invasion and metastasis. Over forty years ago, Judah Folkman hypothesized that targeting tumor angiogenesis would be beneficial for cancer patients. One of the first targets for this new class of drugs was vascular endothelial growth factor (VEGF) a predominant mediator of physiological and pathological angiogenesis. Bevacizumab (Avastin®, Genentech/Roche), a humanized monoclonal antibody that recognizes human VEGF and blocks VEGF from binding to VEGF receptor (VEGFR) 1 and 2, was the first anti-angiogenic drug approved by the United States Food and Drug Administration for the treatment of cancer and remains the gold standard for this class of therapeutics. The Brekken laboratory, in collaborations with Peregrine Pharmaceuticals and Affitech A/S has generated a fully human monoclonal antibody, r84 that recognizes mouse and human VEGF and blocks VEGF binding only to VEGFR2. The data presented in the first half of this dissertation demonstrate the specificity of r84 for VEGF in vitro and in vivo, the efficacy of r84 to control tumor growth and the superior safety profile of r84 as compared to bevacizumab. Although anti-angiogenic therapy was highly anticipated to have great success in patients, overall results have been somewhat disappointing with modest improvements in patient progression free survival and few improvements to overall survival. In addition, with the expanding use of anti-angiogenic drugs such as bevacizumab and a host of receptor tyrosine kinase inhibitors in the clinic, it is becoming increasingly apparent that not all tumors respond or maintain sensitivity to treatment. Therefore, it is increasingly important to identify mechanisms of resistance to anti-angiogenic therapy so that new drug targets can be identified and/or patients can be appropriately screened for markers that can predict for resistance or sensitivity to anti-angiogenic therapy de novo. Non-small cell lung cancer (NSCLC), the most common form of lung cancer, claims the most new diagnoses and cancer-related deaths than any other cancer worldwide and the therapeutic options currently available for this disease, including bevacizumab have done little to change this statistic. The latter half of this thesis focuses on the in vivo screening of human NSCLC cell lines to identify mechanisms of resistance to the anti-angiogenic monoclonal antibodies bevacizumab and r84 in non-small cell lung cancer. Advisors/Committee Members: Brekken, Rolf A..

Subjects/Keywords: Antineoplastic Agents; Neoplasms; Antibodies, Monoclonal

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sullivan, L. A. (2012). Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1040

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sullivan, Laura Anne. “Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 02, 2021. http://hdl.handle.net/2152.5/1040.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sullivan, Laura Anne. “Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.” 2012. Web. 02 Mar 2021.

Vancouver:

Sullivan LA. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2152.5/1040.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sullivan LA. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1040

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

25. Marcus, Claire E. The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility.

Degree: MS, Pathology, 2020, Boston University

URL: http://hdl.handle.net/2144/41291

► Antisperm antibodies (ASA) are thought to be a predominate cause of immune infertility by interfering with various aspects of sperm function in both the male… (more)

▼ Antisperm antibodies (ASA) are thought to be a predominate cause of immune infertility by interfering with various aspects of sperm function in both the male and the female reproductive tracts. The precise mechanism by which these antibodies contribute to infertility, as well as their etiology, remains to be established. ASA are present in a variety of biological substrates, such as genital tract secretions, and the blood sera of both males and females. Although not all ASA underly infertility, a substantial body of research suggests that certain ASA, referred to as sperm immobilizing antibodies (SI-Abs) and sperm agglutinating antibodies, significantly impair sperm transportation in the female reproductive tract. High titers of sperm agglutinating or sperm immobilizing antibodies have been associated with reproductive failure. CD52g is a GPI anchored glycoprotein found on mature sperm and in seminal plasma (SP). Antibodies against a male reproductive tract-specific epitope of CD52g are known to readily agglutinate sperm. The current study sought to develop an ELISA to quantify the prevalence of CD52g antibodies in the sera of male and female patients with infertility, and to determine if there was a correlation between the prevalence of CD52g antibodies and the prevalence of sperm agglutinating antibodies in the sera of these patients. Ultimately, CD52g antibodies were only detected in the sera of patients (21%) with sperm agglutinating antibodies. While detecting CD52g antibodies in sera via an ELISA proved challenging, the results of this study corroborate research demonstrating that CD52g antibodies have a remarkable capacity to agglutinate sperm. Elucidation of the mechanisms underlying this immune response would advance our understanding of immune modulation in human reproductive tracts, further the diagnosis of immune infertility, and are currently providing the basis for the development of a potent dual purpose immunocontraceptive, that both prevents unintended pregnancy, and prevents the transmission of sexually transmitted infections (STIs). Advisors/Committee Members: Anderson, Deborah (advisor), Duffy, Elizabeth (advisor).

Subjects/Keywords: Immunology; Antisperm antibodies; Infertility

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marcus, C. E. (2020). The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/41291

Chicago Manual of Style (16^th Edition):

Marcus, Claire E. “The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility.” 2020. Masters Thesis, Boston University. Accessed March 02, 2021. http://hdl.handle.net/2144/41291.

MLA Handbook (7^th Edition):

Marcus, Claire E. “The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility.” 2020. Web. 02 Mar 2021.

Vancouver:

Marcus CE. The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility. [Internet] [Masters thesis]. Boston University; 2020. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2144/41291.

Council of Science Editors:

Marcus CE. The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility. [Masters Thesis]. Boston University; 2020. Available from: http://hdl.handle.net/2144/41291

University of California – Santa Cruz

26. Fedechkin, Stanislav. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.

Degree: Chemistry, 2018, University of California – Santa Cruz

URL: http://www.escholarship.org/uc/item/21c5j93j

► Respiratory syncytial virus (RSV) is a top cause of severe lower respiratory tract disease and mortality in young children and the elderly. The viral envelope… (more)

Subjects/Keywords: Biochemistry; Antibodies; Attachment; G; RSV

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fedechkin, S. (2018). Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/21c5j93j

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fedechkin, Stanislav. “Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.” 2018. Thesis, University of California – Santa Cruz. Accessed March 02, 2021. http://www.escholarship.org/uc/item/21c5j93j.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fedechkin, Stanislav. “Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.” 2018. Web. 02 Mar 2021.

Vancouver:

Fedechkin S. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. [Internet] [Thesis]. University of California – Santa Cruz; 2018. [cited 2021 Mar 02]. Available from: http://www.escholarship.org/uc/item/21c5j93j.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fedechkin S. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. [Thesis]. University of California – Santa Cruz; 2018. Available from: http://www.escholarship.org/uc/item/21c5j93j

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

27. Bhanot, Anisha. Maternal antibodies in autism: what is known and future directions.

Degree: MS, Medical Sciences, 2018, Boston University

URL: http://hdl.handle.net/2144/30896

► Autism spectrum disorder (ASD) refers to a highly prevalent neuropsychiatric disorder, currently affecting one in every 68 children. ASD is understood as a heterogeneous disorder,… (more)

Subjects/Keywords: Neurosciences; Antibodies; Autism; Immunology; Neurodevelopment

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bhanot, A. (2018). Maternal antibodies in autism: what is known and future directions. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/30896

Chicago Manual of Style (16^th Edition):

Bhanot, Anisha. “Maternal antibodies in autism: what is known and future directions.” 2018. Masters Thesis, Boston University. Accessed March 02, 2021. http://hdl.handle.net/2144/30896.

MLA Handbook (7^th Edition):

Bhanot, Anisha. “Maternal antibodies in autism: what is known and future directions.” 2018. Web. 02 Mar 2021.

Vancouver:

Bhanot A. Maternal antibodies in autism: what is known and future directions. [Internet] [Masters thesis]. Boston University; 2018. [cited 2021 Mar 02]. Available from: http://hdl.handle.net/2144/30896.

Council of Science Editors:

Bhanot A. Maternal antibodies in autism: what is known and future directions. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/30896

University of Dundee

28. Wong, Julin. Targeting c-Met for therapy.

Degree: PhD, 2011, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820

► c-Met is a tyrosine receptor kinase which is activated by its only ligand, the hepatocyte growth factor (HGF). Activation of c-Met leads to a wide… (more)

Subjects/Keywords: 616.994; c-Met; Antibodies; HGFR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, J. (2011). Targeting c-Met for therapy. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820

Chicago Manual of Style (16^th Edition):

Wong, Julin. “Targeting c-Met for therapy.” 2011. Doctoral Dissertation, University of Dundee. Accessed March 02, 2021. https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820.

MLA Handbook (7^th Edition):

Wong, Julin. “Targeting c-Met for therapy.” 2011. Web. 02 Mar 2021.

Vancouver:

Wong J. Targeting c-Met for therapy. [Internet] [Doctoral dissertation]. University of Dundee; 2011. [cited 2021 Mar 02]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820.

Council of Science Editors:

Wong J. Targeting c-Met for therapy. [Doctoral Dissertation]. University of Dundee; 2011. Available from: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820

29. YAP PENG KANG. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.

Degree: 1996, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/153065

Subjects/Keywords: Monoclonal antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

KANG, Y. P. (1996). STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/153065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

KANG, YAP PENG. “STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.” 1996. Thesis, National University of Singapore. Accessed March 02, 2021. https://scholarbank.nus.edu.sg/handle/10635/153065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

KANG, YAP PENG. “STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.” 1996. Web. 02 Mar 2021.

Vancouver:

KANG YP. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. [Internet] [Thesis]. National University of Singapore; 1996. [cited 2021 Mar 02]. Available from: https://scholarbank.nus.edu.sg/handle/10635/153065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

KANG YP. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. [Thesis]. National University of Singapore; 1996. Available from: https://scholarbank.nus.edu.sg/handle/10635/153065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

30. Setyabudi, Mariane. Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies.

Degree: MS, Clinical Laboratory Sciences, 2008, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:16644

Subjects/Keywords: Receptor antibodies

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Setyabudi, M. (2008). Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:16644

Chicago Manual of Style (16^th Edition):

Setyabudi, Mariane. “Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies.” 2008. Masters Thesis, Michigan State University. Accessed March 02, 2021. http://etd.lib.msu.edu/islandora/object/etd:16644.

MLA Handbook (7^th Edition):

Setyabudi, Mariane. “Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies.” 2008. Web. 02 Mar 2021.

Vancouver:

Setyabudi M. Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies. [Internet] [Masters thesis]. Michigan State University; 2008. [cited 2021 Mar 02]. Available from: http://etd.lib.msu.edu/islandora/object/etd:16644.

Council of Science Editors:

Setyabudi M. Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies. [Masters Thesis]. Michigan State University; 2008. Available from: http://etd.lib.msu.edu/islandora/object/etd:16644

Open Access Theses and Dissertations