You searched for subject:(Antibodies)
.
Showing records 1 – 30 of
1917 total matches.
◁ [1] [2] [3] [4] [5] … [64] ▶

University of Texas – Austin
1.
Carroll, Sean Matthew.
Strategies for generating therapeutic antibodies.
Degree: PhD, Chemical Engineering, 2012, University of Texas – Austin
URL: http://hdl.handle.net/2152/ETD-UT-2012-08-5990
► Monoclonal antibodies have become essential therapeutic tools and currently dominate the therapeutic protein market. Consequently, there is continued demand for new therapeutic antibodies and their…
(more)
▼ Monoclonal
antibodies have become essential therapeutic tools and currently dominate the therapeutic protein market. Consequently, there is continued demand for new therapeutic
antibodies and their discovery techniques.
In one part of this work, we report the discovery of a new therapeutic antibody candidate with a novel mechanism for inhibition of a therapeutically relevant biochemical pathway: the classical complement pathway. In order to inhibit classical complement, an antibody was developed that modulates the signaling subcomponent of the pathway initiating C1 complex, C1s. This work includes novel protocols and strategies used for discovery and characterization of antibody D, which binds and inhibits C1s protease activity. By regulating C1s activity, antibody D is shown to regulate classical complement. It is further shown that affinity maturation of antibody D results in higher levels of complement inhibition at various antibody concentrations. This work marks the first example of an antibody that specifically regulates the classical complement pathway by targeting the C1s protease on the pathway initiating C1-complex.
Next, we characterize the human immune cells produced by humanized NSG mice, most notably the B and T lymphocytes, engraftment with human CD34+ HSC cells. We detected development of naïve human B and T cells and their various subtypes, as well as other human immune cells from engrafted mice. However, attempts to generate a robust antibody response to antigens were unsuccessful. Therefore, we conclude that NSG humanized mice developed in this study are suitable for studying the antibody repertoire of naïve B cells, however they are not suitable for the analysis of activated B-cells.
Last, we introduce a novel strategy for the generation of polarized antibody repertoires for use in therapeutic monoclonal antibody discovery. This technique combines targeted antigen delivery to a specific lymph node and a frequency based antibody selection approach in order to directly select antigen specific
antibodies in silico. By directly selecting antigen-specific
antibodies, this approach circumvents laborious and time consuming screening techniques. We expect that this work will be the foundation of an overall improved protocol for monoclonal antibody discovery that accelerates the speed and enhances the simplicity of discovery techniques.
Advisors/Committee Members: Iverson, Brent L. (advisor), Georgiou, George (advisor), Alper, Hal (committee member), Maynard, Jennifer (committee member), Tucker, Philip (committee member).
Subjects/Keywords: Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Carroll, S. M. (2012). Strategies for generating therapeutic antibodies. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2012-08-5990
Chicago Manual of Style (16th Edition):
Carroll, Sean Matthew. “Strategies for generating therapeutic antibodies.” 2012. Doctoral Dissertation, University of Texas – Austin. Accessed March 02, 2021.
http://hdl.handle.net/2152/ETD-UT-2012-08-5990.
MLA Handbook (7th Edition):
Carroll, Sean Matthew. “Strategies for generating therapeutic antibodies.” 2012. Web. 02 Mar 2021.
Vancouver:
Carroll SM. Strategies for generating therapeutic antibodies. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2012. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2152/ETD-UT-2012-08-5990.
Council of Science Editors:
Carroll SM. Strategies for generating therapeutic antibodies. [Doctoral Dissertation]. University of Texas – Austin; 2012. Available from: http://hdl.handle.net/2152/ETD-UT-2012-08-5990

Vanderbilt University
2.
Briney, Bryan Scott.
The development and genetic origin of broadly neutralizing HIV antibodies.
Degree: PhD, Microbiology and Immunology, 2012, Vanderbilt University
URL: http://hdl.handle.net/1803/13651
► Several of the most broadly neutralizing HIV antibodies (bnAbs) contain unique genetic or structural elements, including long heavy chain complementarity determining region 3 (HCDR3) loops…
(more)
▼ Several of the most broadly neutralizing HIV
antibodies (bnAbs) contain unique genetic or structural elements, including long heavy chain complementarity determining region 3 (HCDR3) loops and extensive somatic hypermutation. Two exceptionally broad, potently neutralizing HIV-specific
antibodies, PG9 and PG16, encode HCDR3 loops that are among the longest of any antigen-specific antibody described to date. Passive immunization with two other bnAbs that encode long HCDR3s, 4E10 and 2F5, is able to protect against HIV infection. Induction of such long HCDR3
antibodies may be critical to the design of an effective vaccine strategy for HIV, however it is unclear at present how to induce such
antibodies.
There has been speculation that
antibodies with long HCDR3s are generated primarily through the accumulation of somatic hypermutation-associated insertions. These short insertion events are rare, and design of an immunogen that efficiently induces many such insertions in a single antibody sequence is likely to be extremely difficult. Through the use of high-throughput antibody sequencing, I have identified genetic evidence that these long HCDR3
antibodies are typically formed at the original recombination event, not through accumulation of somatic hypermutation-induced insertions.
Antibodies with long HCDR3s were found in all tested individuals, and long HCDR3
antibodies typically use a restricted subset of D and J gene segments, resulting in the incorporation of highly conserved genetic elements in the majority of such antibody sequences. This work provides an important first step toward the realization of a vaccine that efficiently induces broadly neutralizing HIV
antibodies with long HCDR3s by identifying a conserved genetic target through which B cells encoding
antibodies with long HCDR3s may be induced selectively through vaccination.
Advisors/Committee Members: Billy G. Hudson (committee member), Christopher R. Aiken (committee member), Spyros A. Kalams (committee member), James E. Crowe, Jr. (committee member), James W. Thomas (Committee Chair).
Subjects/Keywords: antibodies; HIV
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Briney, B. S. (2012). The development and genetic origin of broadly neutralizing HIV antibodies. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13651
Chicago Manual of Style (16th Edition):
Briney, Bryan Scott. “The development and genetic origin of broadly neutralizing HIV antibodies.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 02, 2021.
http://hdl.handle.net/1803/13651.
MLA Handbook (7th Edition):
Briney, Bryan Scott. “The development and genetic origin of broadly neutralizing HIV antibodies.” 2012. Web. 02 Mar 2021.
Vancouver:
Briney BS. The development and genetic origin of broadly neutralizing HIV antibodies. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1803/13651.
Council of Science Editors:
Briney BS. The development and genetic origin of broadly neutralizing HIV antibodies. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/13651

Boston University
3.
Russell, Anthony.
Antibodies involved in homograft rejection.
Degree: MA, Biology, 1960, Boston University
URL: http://hdl.handle.net/2144/24569
Subjects/Keywords: Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Russell, A. (1960). Antibodies involved in homograft rejection. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/24569
Chicago Manual of Style (16th Edition):
Russell, Anthony. “Antibodies involved in homograft rejection.” 1960. Masters Thesis, Boston University. Accessed March 02, 2021.
http://hdl.handle.net/2144/24569.
MLA Handbook (7th Edition):
Russell, Anthony. “Antibodies involved in homograft rejection.” 1960. Web. 02 Mar 2021.
Vancouver:
Russell A. Antibodies involved in homograft rejection. [Internet] [Masters thesis]. Boston University; 1960. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2144/24569.
Council of Science Editors:
Russell A. Antibodies involved in homograft rejection. [Masters Thesis]. Boston University; 1960. Available from: http://hdl.handle.net/2144/24569

McGill University
4.
Pan, Bin-Tao.
Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.
Degree: PhD, Department of Biochemistry, 1982, McGill University
URL: https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf
;
https://escholarship.mcgill.ca/concern/theses/8c97ks866
► Des antigènes spécifiques de la surface membranaire de réticulocytes de mouton ont été isoles en utilisant des anticorps spécifiquement développés contre la surface cellulaire. Le…
(more)
▼ Des antigènes spécifiques de la surface membranaire de réticulocytes de mouton ont été isoles en utilisant des anticorps spécifiquement développés contre la surface cellulaire. Le récepteur de la transferrine a été identifié comme l'antigène spécifique majeur des membranes. La caractérisation du récepteur a permis de déterminer un poids moléculaire d'environ 93,000 pour sa forme monomérique et qu'il existe probablement in situ sous une forme dimérique. Il a été aussi établi que les sites antigéniques diffèrent de ceux impliques pour la liaison avec la transferrine. De plus, mous avons montré que la quantité de récepteurs de la transferrine décroit pour éventuellement devenir nulle lorsque les réticulocytes sont cultivés in vitro. […]
Sheep reticulocyte surface specific antigens have been isolated by anti-reticulocyte surface specific antibodies. Moreover, the major specific antigen has been identified as the transferrin receptor. Characterization of the receptor has shown it has a monomeric molecular weight of approximately 93,000 and that it probably exists as a dimer in situ. It has been shown that the antigenic sites differ from the transferrin binding sites and that the receptor spans the membrane. Moreover, it has been shown the transferrin receptors decrease and disappear when reticulocytes are cultured in vitro. […]
Advisors/Committee Members: Johnstone, Rose M..
Subjects/Keywords: Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pan, B. (1982). Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866
Chicago Manual of Style (16th Edition):
Pan, Bin-Tao. “Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.” 1982. Doctoral Dissertation, McGill University. Accessed March 02, 2021.
https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866.
MLA Handbook (7th Edition):
Pan, Bin-Tao. “Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes.” 1982. Web. 02 Mar 2021.
Vancouver:
Pan B. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. [Internet] [Doctoral dissertation]. McGill University; 1982. [cited 2021 Mar 02].
Available from: https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866.
Council of Science Editors:
Pan B. Identification of Reticulocyte Surface Specific Antigens: Their Induced Redistribution and Externalization by the Specific Antibodies in Sheep Reticulocytes. [Doctoral Dissertation]. McGill University; 1982. Available from: https://escholarship.mcgill.ca/downloads/qb98mh92c.pdf ; https://escholarship.mcgill.ca/concern/theses/8c97ks866

University of New South Wales
5.
Mi, Yang.
Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.
Degree: Graduate School of Biomedical Engineering, 2014, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/54227
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true
► Angiogenesis relies on the coordination of endothelial cells, smooth muscle cells and the vascular extracellular matrix around cells. Perlecan is an important heparan sulfate proteoglycan,…
(more)
▼ Angiogenesis relies on the coordination of endothelial cells, smooth muscle cells and the vascular extracellular matrix around cells. Perlecan is an important heparan sulfate proteoglycan, which is widely expressed in vascular tissues and essentially involved in angiogenesis. Perlecan exhibits pro- and anti-angiogenic effects differently according to the cell line from which it was derived from and various structures especially its dependent glycosaminoglycans. The aim of the thesis was to generate and characterise monoclonal antibodies against specific domains of perlecan, and investigate their functions in modulating angiogenesis.lmmunopurified human endothelial cell-derived perlecan decorated with heparin sulfate and recombinant perlecan domain V decorated with chondroitin sulfate were characterised by Enzyme linked immunosorbent assay, immunocytochemistry and Western blotting, and then used as antigens for hybridoma screening. There were 205 single clones generated by limiting dilution, while 174 clones were able to recognise endothelial perlecan. 48 clones with high viability and lg production were tested with intact perlecan and Hep Ill treated perlecan, while they showed different immunoreactive changes between two antigens. However, none of the clones reacted with recombinant perlecan domain I or domain V.Of the newly generated monoclonal antibodies (mAbs), 5 mAbs showed potential to modulate the adhesion of endothelial cells and smooth muscle cells, especially 8C8 and 4F2 reduced the adhesion of both cell types. In addition, the 5 mAbs displayed strong immunoreactivity with perlecan derived from HCAECs and HVSMCs in Western blotting and were able to detect the perlecan expressed on two types of cells in immunocytochemistry. In spreading assay, all 5 mAbs were found to inhibit the HVSMC and HCAEC spreading to different degrees. Moreover, the 5 mAbs except 5G8 showed inhibitory effects in HCAEC migration, while only 8C8, 4F7 and 4F2 were able to reduce the migration of HVSMCs. These 5 mAbs could be useful to investigate the presence and structure of perlecan as primary antibodies, since they were able to recognise the perlecan protein core. They could also become potential tools to explain the functions of different domains of perlecan in angiogenesis and developed as immunotherapeutic drugs to mediate angiogenesis in clinical treatments.
Subjects/Keywords: Antibodies; Perlecan
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mi, Y. (2014). Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Mi, Yang. “Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.” 2014. Masters Thesis, University of New South Wales. Accessed March 02, 2021.
http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true.
MLA Handbook (7th Edition):
Mi, Yang. “Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration.” 2014. Web. 02 Mar 2021.
Vancouver:
Mi Y. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. [Internet] [Masters thesis]. University of New South Wales; 2014. [cited 2021 Mar 02].
Available from: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true.
Council of Science Editors:
Mi Y. Production and characterisation of perlecan antibodies that influence vascular cell adhesion and migration. [Masters Thesis]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/54227 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:13496/SOURCE02?view=true

Rutgers University
6.
Prabhu, Siddharth, 1995-.
Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.
Degree: MS, Chemical and Biochemical Engineering, 2018, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/
► Monoclonal antibodies are the fastest growing segment of pharmaceutical molecules. Currently, they are used as diagnostics, therapeutics for various medical uses as well as in…
(more)
▼ Monoclonal
antibodies are the fastest growing segment of pharmaceutical molecules. Currently, they are used as diagnostics, therapeutics for various medical uses as well as in protein purification. They are among the costliest drugs available in the market. In recent years, due to the competitive pharmaceutical market and incentives for antibody development, biotech industries are investing in novel and advanced technologies to increase the productivity as well as the efficiency of the process.
This project discusses the use of commercially available simulation and scheduling tools to increase the efficiency of the manufacturing process based on monoclonal antibody (mAb). SuperPro Designer and SchedulePro (Intelligen, Inc) is used as a recipe based scheduling tool while VirtECS Scheduler (APC, Inc) is used as a mathematical optimization tool. The manufacturing facility of Eli Lilly and Company located in Kinsale, Ireland is modeled for this thesis. A comparison of these two tools to determine an optimal schedule is obtained. The results show detailed equipment tracking, increased scheduling flexibility, faster facility fit, real-time scheduling and automatic conflict resolution. Increasing the efficiency of the process as well as playing a significant role in day-to-day activities and therefore saving valuable employee time.
Advisors/Committee Members: Ramachandran, Rohit (chair), Ierapetritou, Marianthi (internal member), Singh, Ravendra (internal member), School of Graduate Studies.
Subjects/Keywords: Monoclonal antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Prabhu, Siddharth, 1. (2018). Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/59136/
Chicago Manual of Style (16th Edition):
Prabhu, Siddharth, 1995-. “Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.” 2018. Masters Thesis, Rutgers University. Accessed March 02, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/59136/.
MLA Handbook (7th Edition):
Prabhu, Siddharth, 1995-. “Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody.” 2018. Web. 02 Mar 2021.
Vancouver:
Prabhu, Siddharth 1. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. [Internet] [Masters thesis]. Rutgers University; 2018. [cited 2021 Mar 02].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/.
Council of Science Editors:
Prabhu, Siddharth 1. Practical application of simulation and campaign scheduling of the manufacturing process based on monoclonal antibody. [Masters Thesis]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59136/

University of Utah
7.
Peterson, Lisa K.
Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.
Degree: PhD, Pathology;, 2007, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453
► Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is an autoimmune model of MS, induced by…
(more)
▼ Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Experimental autoimmune encephalomyelitis (EAE) is an autoimmune model of MS, induced by sensitization with myelin proteins of their encephalitogenic peptides. Sensitization with amino acids 92-106 of myelin oligodendrocyte glycoprotein (MOG) induces different disease courses and neuropathology in A.SW and SJL/J mice. SJL/J mice develop a relapsing-remitting (RR-EAE) disease course characterized by mild demyelinating disease with perivascular T cell infiltration. In contrast, A.SW mice develop a progressive (P-EAE) disease course characterized by large areas of plaque-like demyelination with Ig deposition, polymorphonuclear (PMN) cell infiltration and minimal T cell infiltration and high MOG antibody titers in the serum. This thesis describes, as part of the elucidation of the role of antibody in determining disease course and neuropathology, the generation and characterization of hybridomas producing antibody reactive with MOG92-106. Two MOG92-106 hybridomas produced polyreactive IgM antibodies encoded by immunoglobulin genes in their germline configuration, indicating that the antibodies are natural autoantibodies (NAA). These MOG92-106 reactive NNA bind myelin and cause demyelination in vivo, suggesting that they could contribute to the pathogenesis of P-EAE. In addition, sensitization of A.S.W. mice with MOG92-106 or intraperitoneal injection of the MOG92-106 reactive hybridomas resulted in antibody deposition in glomeruli of kidneys and proteinuria, demonstrating that MOG92-106 reacative NAA can induce systemic autoimmunity. However, depletion of B-1 cells, the main produces of NAA, had minimal effects on the development of demyelination, antibody deposition or disease progression in mice sensitized with MOG92-106. These results indicate that while MOG92-106 reacative NAA are capable of inducing pathology in the CNS and kidneys, they play a minor role in the pathogenesis of MOG92-106-infduced EAE. In addition, this thesis describes induction of another combination of disease course and neuropathology in B10.S mice. B10.S mice have previously been considered resistant to EAE, but sensitization with MOG92-106 resulted in neuropathology in 93% of the mice and monophasic or relapsing-remitting disease course in 30% of the mice. Taken together, this work expands the spectrum of autoimmune disease that can be induced and investigated using MOG92-106.
Subjects/Keywords: Multiple Sclerosis; Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Peterson, L. K. (2007). Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453
Chicago Manual of Style (16th Edition):
Peterson, Lisa K. “Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.” 2007. Doctoral Dissertation, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453.
MLA Handbook (7th Edition):
Peterson, Lisa K. “Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein.” 2007. Web. 02 Mar 2021.
Vancouver:
Peterson LK. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. [Internet] [Doctoral dissertation]. University of Utah; 2007. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453.
Council of Science Editors:
Peterson LK. Expanding the spectrum of autoimmunity induced with myelin oligodendrocyte glycoprotein. [Doctoral Dissertation]. University of Utah; 2007. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1130/rec/453

Tulane University
8.
Yenni, Rachael.
Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.
Degree: 2016, Tulane University
URL: https://digitallibrary.tulane.edu/islandora/object/tulane:75232
► The humoral arm of the adaptive immune system involves the production of antibodies by cells of the B cell lineage, which bind and in some…
(more)
▼ The humoral arm of the adaptive immune system involves the production of antibodies by cells of the B cell lineage, which bind and in some cases neutralize or enhance infectivity of pathogens, including viruses. Humoral immune responses to each of the Lassa virus (LASV) structural proteins have been detected[1, 2]. However, there have been few efforts to perform fine structure epitope mapping of the antigenic sites recognized by LASV-specific antibodies. Murine monoclonal antibodies (MAbs) have been produced against several arenaviruses [1-4]. Some MAbs to Lassa virus proteins react broadly with arenaviruses demonstrating there may be an epitope or epitopes conserved among arenaviruses. Neutralizing antibody epitopes of LASV recognized by humans remains essentially unexplored. Therefore, my project will focus on identifying, characterizing and better understanding the antigen-antibody binding relationship of LASV structural proteins. We hypothesize that humans exposed to LASV differ quantitatively and qualitatively in their ability to produce antibodies that recognize potential binding epitopes on LASV proteins, and these differences can be explored via the identification and characterization of these epitopes using LASV recombinant proteins and synthetic peptides. We expect that a panel of unique human MAbs that bind specifically to LASV and LASV recombinant proteins will be isolated and prove to be valuable tools in characterizing the humoral response to LASV. The human MAbs must be characterized to determine how binding occurs. A fundamental understanding of mechanisms of antibody binding of LASV may have significant implications for the generation of antibody-based therapeutics
1
Rachael Yenni
Advisors/Committee Members: (author), Garry, Robert (Thesis advisor), (Thesis advisor), School of Medicine Biomedical Sciences Graduate Program (Degree granting institution), NULL (Degree granting institution).
Subjects/Keywords: Lassa antibodies epitopes
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yenni, R. (2016). Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:75232
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yenni, Rachael. “Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.” 2016. Thesis, Tulane University. Accessed March 02, 2021.
https://digitallibrary.tulane.edu/islandora/object/tulane:75232.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yenni, Rachael. “Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins.” 2016. Web. 02 Mar 2021.
Vancouver:
Yenni R. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. [Internet] [Thesis]. Tulane University; 2016. [cited 2021 Mar 02].
Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:75232.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yenni R. Identification and Characterization of Lassa virus specific antibodies that recognize epitopes on Lassa virus recombinant proteins. [Thesis]. Tulane University; 2016. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:75232
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida Atlantic University
9.
Cavallo, Michelle Fay.
ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS.
Degree: 2019, Florida Atlantic University
URL: http://fau.digital.flvc.org/islandora/object/fau:42161
► Two novel methodologies were developed for purification and functional (DNA hydrolytic) assessment of anti-DNA antibodies of IgG isotype from patients with Systemic Lupus Erythematosus (SLE).…
(more)
▼ Two novel methodologies were developed for purification and functional (DNA hydrolytic) assessment of anti-DNA antibodies of IgG isotype from patients with Systemic Lupus Erythematosus (SLE). Earlier protocols for purification and analysis of antibody hydrolytic abilities were lengthy, laborious, and potentially disruptive to antibody function. Purification protocols failed to capture all four IgG subclasses and produced multiple bands outside the range of IgG on electrophoretic separation. Hydrolysis assays were discontinuous increasing the likelihood of introducing error and making them better suited to analysis of endpoint kinetics rather than reaction kinetics.
A two-step, affinity-based purification protocol was developed which utilized magnetic Dynabeads to capture serum components with binding affinity for a thymine 20mer followed by capture of the antibody components of this initial anti-T 20mer serum fraction using Protein G. A fluorescence-based method for real-time, continuous analysis of anti-DNA antibody hydrolytic activity utilizing hydrolysis probes was developed and used to characterize abzyme reaction kinetic parameters. Anti-DNA antibodies demonstrated significantly different Vmax and Km values in the hydrolysis assay (p <0.001) when compared with a DNAse I control.
2019
Degree granted: Dissertation (Ph.D.) – Florida Atlantic University, 2019.
Collection: FAU
Advisors/Committee Members: Hartmann, James X. (Thesis advisor), Florida Atlantic University (Degree grantor), Department of Biological Sciences, Charles E. Schmidt College of Science.
Subjects/Keywords: Systemic lupus erythematosus; Anti-DNA antibodies; DNA antibodies; Antibodies, Catalytic; Autoantibodies – Analysis; Antibodies – isolation & purification
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cavallo, M. F. (2019). ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS. (Thesis). Florida Atlantic University. Retrieved from http://fau.digital.flvc.org/islandora/object/fau:42161
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cavallo, Michelle Fay. “ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS.” 2019. Thesis, Florida Atlantic University. Accessed March 02, 2021.
http://fau.digital.flvc.org/islandora/object/fau:42161.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cavallo, Michelle Fay. “ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS.” 2019. Web. 02 Mar 2021.
Vancouver:
Cavallo MF. ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS. [Internet] [Thesis]. Florida Atlantic University; 2019. [cited 2021 Mar 02].
Available from: http://fau.digital.flvc.org/islandora/object/fau:42161.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cavallo MF. ANTI-DNA ANTIBODIES IN SYSTEMIC LUPUS. [Thesis]. Florida Atlantic University; 2019. Available from: http://fau.digital.flvc.org/islandora/object/fau:42161
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge
10.
Peris, Daniela.
In vitro diversification of chimeric human/chicken antibodies.
Degree: PhD, 2019, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/293408
► Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and refining affinities and specificities of existing ones (Cumbers et…
(more)
▼ Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and refining affinities and specificities of existing ones (Cumbers et al., 2002). The chicken derived DT40 B cell line has been adapted for the expression and diversification of humanized antibodies. Human antibodies in the form of scFvs expressed in the surface of these cells have been successfully diversified to produce a scFv library, from which antibodies of desired specificity and improved affinity can be evolved using a combination of flow cytometry (FACS) and magnetic beads (MACS) (Lim et al., 2016).
In this thesis I further developed the system by finding an alternative approach to antibody humanization and evolution that overcomes the obstacles encountered during the evolution of human scFvs in DT40. I tested different approaches and, although presenting new challenges, I found that chimeric human/chicken antibodies could be engineered and expressed in this cell line and overcame many of the problems experienced with scFvs evolution. This approach was used to generate a combinatorial library for the screening of novel antibodies and to introduce V genes of antibodies of known specificity that could be evolved for improved affinity. In addition to the engineering of the DT40 cell line, and with the aim to obtain a sustainable antibody diversification rate over time, I generated a cell cycle-regulated, nuclear restricted version of Activation Induced Deaminase AID that yields high rates of mutations in chimeric human/chicken antibodies.
To conclude, a new and promising approach for antibody evolution in DT40 was developed. The new system posed new challenges that were identified and either solved or used to outlined future directions for the development and improvement of the technique.
Subjects/Keywords: Antibodies; DT40 cells; Antibody engineering; Chimeric antibodies; Antibody evolution; human antibodies; Antibody diversification
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Peris, D. (2019). In vitro diversification of chimeric human/chicken antibodies. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/293408
Chicago Manual of Style (16th Edition):
Peris, Daniela. “In vitro diversification of chimeric human/chicken antibodies.” 2019. Doctoral Dissertation, University of Cambridge. Accessed March 02, 2021.
https://www.repository.cam.ac.uk/handle/1810/293408.
MLA Handbook (7th Edition):
Peris, Daniela. “In vitro diversification of chimeric human/chicken antibodies.” 2019. Web. 02 Mar 2021.
Vancouver:
Peris D. In vitro diversification of chimeric human/chicken antibodies. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Mar 02].
Available from: https://www.repository.cam.ac.uk/handle/1810/293408.
Council of Science Editors:
Peris D. In vitro diversification of chimeric human/chicken antibodies. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/293408

University of Pretoria
11.
[No author].
A resonant mirror biosensor approach to understand
antibody-antigen interactions in Guillain Barré
Syndrome
.
Degree: 2008, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/
► Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and…
(more)
▼ Guillain Barré Syndrome in humans is characterised
by ascending paralysis. It is often associated with preceding
infections two to four weeks prior to nadir and is fatal in five
percent of cases.
Antibodies specific to several nerve components
are frequently associated with clinical symptoms in GBS. These
antibodies were found to be specific to various gangliosides and
ganglioside complexes. It was also found that antibody reactivity
to gangliosides is affected by membrane components. The most
prevalent (20-30%) immunoglobulin in GBS is anti-GM1 (20-30%),
which also binds to the LPS of the PEN O:19 Campylobacter jejuni
serotype. This is the most common infectious agent associated with
GBS and emphasizes the importance of infection and anti-ganglioside
antibodies in disease development. Intravenous infusion of pooled
immunoglobulin from healthy donors, also called intravenous
immunoglobulin (IVIg), halves the severity of disease
manifestation. The action mechanism of IVIg in curing GBS is not
clear, but intravenous immunoglobulin was shown to neutralize
anti-ganglioside binding activity and its pathogenic effects. It
was further found that anti-idiotypic
antibodies in IVIg inhibit
anti-ganglioside antibody activity. Treatment with IVIg is not
equally effective in all GBS cases, which might be due to the
inability of IVIg to neutralize anti-ganglioside
antibodies in all
patients adequately. Therefore, the treatment of GBS with IVIg
needs to be better understood in order to improve its use as a cure
for GBS. This study confirmed previous findings that the
interaction of patient serum anti-GM1
antibodies and ganglioside
auto-antigens is greatly impaired by components in healthy serum.
Bound anti-GM1
antibodies could be displaced by (presumably)
anti-idiotypic
antibodies from healthy donor serum. This study
found that the displacement potential between donor sera differs.
Anti-GM1 antibody displacement was found to be dependent on the
character of both anti-GM1 and anti-idiotypic antibody. This
demonstrated the feasibility of improving the efficiency of
treatment by IVIg by sourcing it from only those sera that test
best for displacing auto-
antibodies from their ganglioside antigens
in ELISA. IVIg selection may therefore greatly benefit from the use
of recombinant phage display
antibodies to distinguish between the
various types of GBS for treatment. To develop a method to
characterize anti-ganglioside
antibodies sensitively, an evanescent
field biosensor was employed in which gangliosides were presented
in a liposome environment. This provided a more physiological way
of antibody antigen recognition. The optimized method determined
the ganglioside binding specificity of purified IgG from a GBS
patient, and mouse monoclonal anti-GM1 and anti-GD1a
antibodies
accurately. The results compared well with those from ELISA. The
results obtained with purified IgG were far better than that
obtained with whole serum analysis. This could be due to
non-specific binding or the presence of inhibiting anti-idiotypic
antibodies in…
Advisors/Committee Members: Prof J A Verschoor (advisor).
Subjects/Keywords: Antibody-antigen interactions;
Antibodies;
UCTD
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2008). A resonant mirror biosensor approach to understand
antibody-antigen interactions in Guillain Barré
Syndrome
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-04172008-111655/
Chicago Manual of Style (16th Edition):
author], [No. “A resonant mirror biosensor approach to understand
antibody-antigen interactions in Guillain Barré
Syndrome
.” 2008. Masters Thesis, University of Pretoria. Accessed March 02, 2021.
http://upetd.up.ac.za/thesis/available/etd-04172008-111655/.
MLA Handbook (7th Edition):
author], [No. “A resonant mirror biosensor approach to understand
antibody-antigen interactions in Guillain Barré
Syndrome
.” 2008. Web. 02 Mar 2021.
Vancouver:
author] [. A resonant mirror biosensor approach to understand
antibody-antigen interactions in Guillain Barré
Syndrome
. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 02].
Available from: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/.
Council of Science Editors:
author] [. A resonant mirror biosensor approach to understand
antibody-antigen interactions in Guillain Barré
Syndrome
. [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-04172008-111655/

University of Pretoria
12.
[No author].
Engineering recombinant chicken antibodies for improved
characteristics
.
Degree: 2009, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/
► Phage libraries are a versatile source of recombinant antibody fragments directed against a wide variety of antigens. Recombinant antibodies have the advantage that they can…
(more)
▼ Phage libraries are a versatile source of
recombinant antibody fragments directed against a wide variety of
antigens. Recombinant
antibodies have the advantage that they can
be engineered to improve their binding or other characteristics. A
chicken single chain variable fragment (scFv) phage library was
panned against the 16 kDa antigen of Mycobacterium tuberculosis.
Three phage displayed
antibodies were obtained which bound
specifically to the antigen. In soluble scFv format, however, they
produced low ELISA signals. For this reason they were able to be
used as models for antibody engineering. Three mutant sub-libraries
were created by random mutagenesis. High stringency panning of the
mutant sub-libraries against the target antigen yielded stronger
binders which produced ELISA signals of up to eleven times higher
than the parent scFvs. An increase in affinity was confirmed by
surface plasmon resonance. One mutant scFv with a single amino acid
exchange also showed an increase in the yield of scFvs it produced.
Upon shortening the linker sequence between the heavy and light
chains, size exclusion chromatography showed that multimerisation
had occurred. Dimers, trimers and tetramers were formed thus
increasing the avidity of the scFvs. Tetramers derived from the
unmutated scFv showed the greatest improvement in ELISA binding. To
improve expression and purification, an alternate bacterial
expression vector with a histidine tag was investigated. For this
series of experiments the coding region for a chicken scFv directed
against VP7 of bluetongue virus was transferred from the pHEN1
display vector to the pSANG 14-3F vector, which fuses the scFv gene
to a bacterial alkaline phosphatase gene. A bi-functional chicken
scFv-alkaline phosphatase fusion protein that exhibits both
alkaline phosphatase activity and specific antigen binding was
expressed in Escherichia coli and purified in a single step via
metal affinity chromatography. Furthermore the scFv-AP fusion
protein was directly detected in ELISA without the use of secondary
detection reagents. This study confirms that the strategies used
can efficiently enhance the characteristics of chicken
scFvs.
Advisors/Committee Members: Dr J Fehrsen (advisor).
Subjects/Keywords: Chicken antibodies;
Tuberculosis;
UCTD
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2009). Engineering recombinant chicken antibodies for improved
characteristics
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-02202009-170916/
Chicago Manual of Style (16th Edition):
author], [No. “Engineering recombinant chicken antibodies for improved
characteristics
.” 2009. Masters Thesis, University of Pretoria. Accessed March 02, 2021.
http://upetd.up.ac.za/thesis/available/etd-02202009-170916/.
MLA Handbook (7th Edition):
author], [No. “Engineering recombinant chicken antibodies for improved
characteristics
.” 2009. Web. 02 Mar 2021.
Vancouver:
author] [. Engineering recombinant chicken antibodies for improved
characteristics
. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Mar 02].
Available from: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/.
Council of Science Editors:
author] [. Engineering recombinant chicken antibodies for improved
characteristics
. [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-02202009-170916/

Universiteit Utrecht
13.
Liang, K.
Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.
Degree: 2010, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/188023
► Biosimilars, defined as biological medicinal products comparable in quality, safety and efficacy to reference products, follow the independent regulatory pathway in the EU for marketing…
(more)
▼ Biosimilars, defined as biological medicinal products comparable in quality, safety and efficacy to reference products, follow the independent regulatory pathway in the EU for marketing authorizations after the patent expirations of the reference products. With the recent advent of biosimilar monoclonal
antibodies (mAbs), the evolving EU guidelines on biosimilars are about to have a new regulatory landscape. The development of guideline on biosimilar mAbs is considered as a regulatory challenge due the tremendous complexity of mAbs. MAbs are highly complex molecules with secondary and tertiary structures
subject to post-translational modifications, which are often heterogeneous and vulnerable to slight change in manufacturing process. Thus, to what extent of the similarity a biosimilar mAb should demonstrate, compared with its reference product, is currently the most controversial regulatory issue. This review discusses this issue by raising the questions in tiers of quality, non-clinical, clinical issues. In principle, the similarity issue on mAbs will be extensively discussed and justified on case-by-case basis. Most importantly, evaluation of biosimilar mAbs should be conducted with a holistic approach, i.e. rigorous interpretation between structure-function relationships to reduce unnecessary clinical trials while providing comprehensive post-marketing risk management plans. In the long run, biobetters might be gradually taking over biosimilars on the established regulatory track and leading to better access to biological medications.
Advisors/Committee Members: G.M. Leufkens, Hubert.
Subjects/Keywords: Biosimilar; monoclonal antibodies (mAbs)
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Liang, K. (2010). Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/188023
Chicago Manual of Style (16th Edition):
Liang, K. “Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.” 2010. Masters Thesis, Universiteit Utrecht. Accessed March 02, 2021.
http://dspace.library.uu.nl:8080/handle/1874/188023.
MLA Handbook (7th Edition):
Liang, K. “Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?.” 2010. Web. 02 Mar 2021.
Vancouver:
Liang K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. [Internet] [Masters thesis]. Universiteit Utrecht; 2010. [cited 2021 Mar 02].
Available from: http://dspace.library.uu.nl:8080/handle/1874/188023.
Council of Science Editors:
Liang K. Biosimilars in the Next Era: Hope or Hype for Biosimilar Monoclonal Antibodies in the Near Future?. [Masters Thesis]. Universiteit Utrecht; 2010. Available from: http://dspace.library.uu.nl:8080/handle/1874/188023

University of Namibia
14.
Hikufe, EH.
Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia
.
Degree: 2016, University of Namibia
URL: http://hdl.handle.net/11070/1696
► Rabies kills over 55,000 people worldwide annually of which about 97% die resulting from the bite(s) of rabid dogs. Despite the free annual vaccination of…
(more)
▼ Rabies kills over 55,000 people worldwide annually of which about 97% die
resulting from the bite(s) of rabid dogs. Despite the free annual vaccination of dogs
in Namibia and Ohangwena region in particular, rabies is still on the increase in both animals and humans. We conducted this study to establish the level of protection against rabies in the vaccinated domestic dogs through antibody testing.
Furthermore, the study assessed the level of people’s knowledge on rabies.
A descriptive cross-sectional study design was used. A random sample of 170 sera
was collected from the dogs after one year from the previous vaccination. We tested
sera at the Central Veterinary Laboratory using the BioPro Rabies ELISA test kit
and defined rabies protective antibody titre as titres ≥0.5IU/mL. Data were collected
using a structured questionnaire and analysed using Epi info 7 and Microsoft excel.
Among the 170 dogs, 136 (80%) acquired protective antibody titres (95% CI: 73.2%-85.7%). The majority of samples came from dogs younger than 3 years 90(53%). However, dogs older than 3 years maintained rabies protective antibodies better than the younger dogs (87% versus 74%), Chi2= 4.2, df=1, P=0.04. About 88% of dogs that received repeated vaccinations (boosters) over the years maintained protective antibodies compared to only 74% of dogs that received a single vaccination a year ago without a booster (P= 0.03). Eighty (80%) of the vaccinated dogs maintained protective rabies antibodies. High level of protective antibodies was observed more in older dogs and dogs that received booster vaccinations over the last three years. We recommend rabies vaccination to be conducted twice per year and forceful vaccination be instituted for stray dogs and dogs that are difficult to handle during the campaigns. Cooperation among relevant stakeholders should be instituted to ensure effective rabies control.
Subjects/Keywords: Rabies
;
Dogs
;
Antibodies
;
Vaccination
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hikufe, E. (2016). Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia
. (Thesis). University of Namibia. Retrieved from http://hdl.handle.net/11070/1696
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hikufe, EH. “Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia
.” 2016. Thesis, University of Namibia. Accessed March 02, 2021.
http://hdl.handle.net/11070/1696.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hikufe, EH. “Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia
.” 2016. Web. 02 Mar 2021.
Vancouver:
Hikufe E. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia
. [Internet] [Thesis]. University of Namibia; 2016. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/11070/1696.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hikufe E. Rabies sero-survey in vaccinated domestic dogs and knowledge assessment of rabies among dog owners, Ohangwena region, Namibia
. [Thesis]. University of Namibia; 2016. Available from: http://hdl.handle.net/11070/1696
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah
15.
Tolley, Neal Dean.
DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.
Degree: MS;, Pathology;, 1997, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347
► Although the etiology of multiple sclerosis (MS) is unknown, several factors are believed to play a role in this disease: genetic components, immunologic elements, and…
(more)
▼ Although the etiology of multiple sclerosis (MS) is unknown, several factors are believed to play a role in this disease: genetic components, immunologic elements, and environmental factors. The best explanation of the latter is viral infection. Chronic Theiler’s murine encephalomyelitis virus (TMEV) infection in susceptible mice is an optimal model, mimicking several aspects of MS. DNA vaccination has been used experimentally to successfully protect animals against chronic viral infection. I created eukaryotic expression vectors with cDNA encoding the TMEV capsid proteins VP1, VP2, VP3, and VP4. I then vaccinated groups of SJL/J mice one, two, or three times with different plasmid construct. This was followed by live TMEV challenge to determine the protective effect, if any, that would be induced by DNA vaccination. It was demonstrated that vaccination of mice three times with DNA encoding VP2 lead to partial protection of mice from disease, demonstrated by a decrease in clinical symptoms and histopathology. Two vaccinations with cDNA encoding VP4 also lead to a decrease in clinical expression of disease. Additionally, mice vaccination one time with cDNA encoding VP1 experiences more serve disease and/or earlier onset clinically and histologically than control. Although there was no correlation between antibody titers and disease course, we could detect anti-capsid protein antibody in mice inoculated with only plasmid. These results indicate that DNA immunization may prevent chronic virus induced demyelinating disease, and in the future may be applied to illnesses such as MS.
Subjects/Keywords: Immunology; Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tolley, N. D. (1997). DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347
Chicago Manual of Style (16th Edition):
Tolley, Neal Dean. “DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.” 1997. Masters Thesis, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347.
MLA Handbook (7th Edition):
Tolley, Neal Dean. “DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;.” 1997. Web. 02 Mar 2021.
Vancouver:
Tolley ND. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. [Internet] [Masters thesis]. University of Utah; 1997. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347.
Council of Science Editors:
Tolley ND. DNA vaccination of SJL/J mice against Theiler's murine encephalomyelitis virus;. [Masters Thesis]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1599/rec/347

University of Utah
16.
Frost, Marisa Antónia.
Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.
Degree: PhD, Pathology;, 2005, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920
► Eosinophils play a dual role in the hose immune response. Whereas considerable evidence exists that are effective in defending the host against parasite infections, they…
(more)
▼ Eosinophils play a dual role in the hose immune response. Whereas considerable evidence exists that are effective in defending the host against parasite infections, they also play an important role in the inflammatory pathology resulting from parasitic infections and allergic reactions. De-granulation of the eosinophil and release of its cytotoxic proteins are important both in the host defense and inflammation. For example, the granule proteins are toxic to parasites as well as normal host tissue. Eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are ribonucleases of the eosinophil granule. We report on studies of a putative novel protein in the eosinophil granules isolated from lysates of human eosinophil granules from a patient with hypereosinophilic syndrome. This putative protein is named Eosinophil Cationic Related Protein (ECRP). We attempted to isolate ECRP from the granule lysates by get filtration and ion exchange chromatography. Chemiluminescent immunodection using polyclonal antibodies revealed a band on western blots which reacted to ECRP antibodies. The sequence homology, conservation of known functional sites, and chromosomal mapping to the same region in chromosome 14 as EDN and ECP strongly suggest that ECRP is also a ribonuclease with functions similar to both ECN and ECP. RCEP has a predicted weight of 14.7KDs and a predicted PI of 9.72. ECRP has 90% identity to EDN in sequence homology. The putative ECRP appears to have an open reading frame that is transcriptional active in response to IL-5. Furthermore, ICRP antibodies reacting to granule lysates suggests that the ECRP protein is likely to be present in eosinophil granules.
Subjects/Keywords: Proteins; Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Frost, M. A. (2005). Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920
Chicago Manual of Style (16th Edition):
Frost, Marisa Antónia. “Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.” 2005. Doctoral Dissertation, University of Utah. Accessed March 02, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920.
MLA Handbook (7th Edition):
Frost, Marisa Antónia. “Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein.” 2005. Web. 02 Mar 2021.
Vancouver:
Frost MA. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. [Internet] [Doctoral dissertation]. University of Utah; 2005. [cited 2021 Mar 02].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920.
Council of Science Editors:
Frost MA. Preliminary investigations of Eosinophil Cationic Related Protein, a putative eosinophil granule protein. [Doctoral Dissertation]. University of Utah; 2005. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/508/rec/920
17.
Ζάβαλη, Μαρία-Δημητρούλα.
Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.
Degree: 2009, University of Patras
URL: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124
► Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε…
(more)
▼ Η παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων παρουσιάζει ιδιαίτερο ενδιαφέρον διότι παρέχει άμεσα πληροφορία σχετικά με την κινητική πρόσδεσης και τις σταθερές ισορροπίας. Σε αυτή την αναφορά, παρουσιάζεται μια μεθοδολογία η οποία βασίζεται στη Φασματοσκοπία Ανάκλασης Λευκού Φωτός και ενδείκνυται για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αντιδράσεων που λαμβάνουν χώρα σε στερεά υποστρώματα. Η οπτική διάταξη αποτελείται από μια VIS–NIR οπτική πηγή η οποία επικοινωνεί μέσω οπτικής ίνας με φασματοφωτόμετρο συνδεδεμένο σε Η/Υ. Το εξωτερικό τμήμα της οπτικής ίνας κατευθύνει το φως κάθετα πάνω στην επιφάνεια όπου συμβαίνουν οι αλληλεπιδράσεις μεταξύ των βιιομορίων. Το ανακλώμενο φως συγκεντρώνεται από το εσωτερικό τμήμα της οπτικής ίνας και κατευθύνεται στο φασματοφωτόμετρο. Οι αντιδράσεις λαμβάνουν χώρα σε επιφάνεια διοξειδίου του πυριτίου επικαλυμμένη με πολυμερικό υμένιο. Μια αντλία χρησιμοποιείται για την παροχή των αντιδραστηρίων με ελεγχόμενο ρυθμό σε ένα μικρορρευστομηχανικό κανάλι. Το φάσμα της ανάκλασης καταγράφεται σε πραγματικό χρόνο. Οι αντιδράσεις μεταξύ των πρωτεϊνικών μορίων γίνονται αντιληπτές σαν μετατοπίσεις του μήκους κύματος εκεί που παρατηρείται το φαινόμενο της ενισχυτικής συμβολής. Οι μετατοπίσεις αυτές στο μήκος κύματος συμβαίνουν λόγω του σχηματισμού πρωτεϊνικού στρώματος είτε κατά τη διάρκεια της προσρόφησης των αντισωμάτων στο στερεό υπόστρωμα ή λόγω αλλαγής στο πάχος του πρωτεϊνικού στρώματος όταν τα συμπληρωματικά αντιγόνα δεσμεύονται από τα ήδη ακινητοποιημένα αντισώματα. Η προτεινόμενη μεθοδολογία εφαρμόστηκε για την παρακολούθηση σε πραγματικό χρόνο, χωρίς τη χρήση ιχνηθέτη της αντίδρασης μεταξύ των συμπληρωματικών μορίων βιοτίνης-στρεπταβιδίνης όπως επίσης και για την ανίχνευση της πρόσδεσης των αντιγόνων mouse IgG από ήδη ακινητοποιημένα αντισώματα anti-mouse IgG. Mouse IgG σε συγκεντρώσεις μικρότερες των 150 pM ανιχνεύτηκαν σε πολύ μικρούς χρόνους αντίδρασης (10 min). Ο οπτικός αισθητήρας είναι μια απλή, γρήγορη, χαμηλού κόστους διάταξη για την παρακολούθηση σε πραγματικό χρόνο των βιομοριακών αλληλεπιδράσεων που τον καθιστά κατάλληλο για διάφορες αναλυτικές εφαρμογές.
Label-free monitoring of biomolecular reactions in real-time is of great interest since it can provide direct results concerning binding kinetics and equilibrium constants. In this report, a method based on White Light Reflectance Spectroscopy (WLRS) is presented that is capable for real-time monitoring of biomolecular reactions taking place on a solid surface. The optical setup consists of a VIS–NIR light source connected through a bifurcated optical fiber to a PC-driven spectrophotometer. The outer part of the optical fibre guides the light vertically onto the surface where the biomolecular reactions occur. The reflected light is collected from the central part of the optical fibre and is directed to the spectrophotometer. The reactions take place on the top of a polymer covered silicon dioxide surface. A microfluidic module in combination with a micropump is used to supply the…
Advisors/Committee Members: Κουτσούρης, Δημήτριος, Zavali, Maria-Dimitroula, Κουτσούρης, Δημήτριος, Νικήτα, Κωνσταντίνα, Μισιακός, Κωνστατίνος.
Subjects/Keywords: Βιοαισθητήρες; Αντισώματα; 610.28; Biosensors; Antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ζάβαλη, . (2009). Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. (Masters Thesis). University of Patras. Retrieved from http://nemertes.lis.upatras.gr/jspui/handle/10889/3124
Chicago Manual of Style (16th Edition):
Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Masters Thesis, University of Patras. Accessed March 02, 2021.
http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.
MLA Handbook (7th Edition):
Ζάβαλη, Μαρία-Δημητρούλα. “Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις.” 2009. Web. 02 Mar 2021.
Vancouver:
Ζάβαλη . Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Internet] [Masters thesis]. University of Patras; 2009. [cited 2021 Mar 02].
Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124.
Council of Science Editors:
Ζάβαλη . Βιοαναλυτικά μικροσυστήματα μεγάλης ακρίβειας βασισμένα σε συμβολομετρικές και ρευστομηχανικές διατάξεις. [Masters Thesis]. University of Patras; 2009. Available from: http://nemertes.lis.upatras.gr/jspui/handle/10889/3124
18.
Khan, Asad.
Studies on experimentally produced Antibodies against
peroxynitrite Modified histones; -.
Degree: Biochemistry, 2008, Aligarh Muslim University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/17961
► Reactive nitrogen and oxygen species are produced under physiological newlineconditions. However, excess of these radicals may damage cellular lipids, proteins and newlinenucleic acids. These reactive…
(more)
▼ Reactive nitrogen and oxygen species are produced
under physiological newlineconditions. However, excess of these
radicals may damage cellular lipids, proteins and newlinenucleic
acids. These reactive species have been implicated in many disease
conditions newlineincluding chronic inflammation, atherosclerosis,
rheumatoid arthritis, some newlineneurodegenerative diseases and
systemic lupus erythematosus. Systemic lupus newlineerythematosus
and rheumatoid arthritis are autoimmune diseases with complex
etiology newlineand pathogenesis. The abnormal level of
nitrotyrosine detected in tissues affected by newlineabove diseases
have been attributed to peroxynitrite mediated hypernitration of
newlinetyrosine residues in proteins. Peroxynitrite is a potent
oxidant as well as nitrating agent newlineand has in vivo
existence. It is formed when nitric oxide reacts with superoxide
radical. newlinePeroxynitrite is a powerful pro-inflammatory
substance and may increase vascular newlinepermeability in inflamed
tissues. newlineIn this doctoral thesis, physico-chemical and
immunological studies have been newlinecarried out on histones
modified by peroxynitrite with an objective of studying the
newlinepossible role of oxidatively nitrated proteins in the
initiation/progression of systemic newlinelupus erythematosus and
rheumatoid arthritis. newlineAnalysis of UV absorption profile of
peroxynitrite modified H1, H2A, H2B newlineand H3 histones revealed
peak shift to higher wavelength and hyperchromicity at 276
newlinenm compared to native histones. Furthermore, in case of
modified histones an newlineadditional peak was observed at 420 nm
which corresponds to nitrotyrosine because a newlinestandard
solution of 3-nitrotyrosine had given a similar peak under our
experimental newlineconditions. The hyperchromicity observed in
peroxynitrite modified histones samples newlinemight be due to
nitration of tyrosine residues which ultimately enhances the molar
newlineabsorptivity compared to native histones. HPLC analysis
confirmed generation of newlinenitrotyrosine in peroxynitrite
modified histones.
Bibliography p.142-160
Advisors/Committee Members: Alam, Khursheed.
Subjects/Keywords: Biotechnology; peroxynitrite; Antibodies; experimentally
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Khan, A. (2008). Studies on experimentally produced Antibodies against
peroxynitrite Modified histones; -. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17961
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Khan, Asad. “Studies on experimentally produced Antibodies against
peroxynitrite Modified histones; -.” 2008. Thesis, Aligarh Muslim University. Accessed March 02, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/17961.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Khan, Asad. “Studies on experimentally produced Antibodies against
peroxynitrite Modified histones; -.” 2008. Web. 02 Mar 2021.
Vancouver:
Khan A. Studies on experimentally produced Antibodies against
peroxynitrite Modified histones; -. [Internet] [Thesis]. Aligarh Muslim University; 2008. [cited 2021 Mar 02].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Khan A. Studies on experimentally produced Antibodies against
peroxynitrite Modified histones; -. [Thesis]. Aligarh Muslim University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17961
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria
19.
Van der Merwe, Hermanus
Daniel.
A resonant
mirror biosensor approach to understand antibody-antigen
interactions in Guillain Barré Syndrome.
Degree: MSc, Biochemistry, 2008, University of Pretoria
URL: http://hdl.handle.net/2263/23995
► Guillain Barré Syndrome in humans is characterised by ascending paralysis. It is often associated with preceding infections two to four weeks prior to nadir and…
(more)
▼ Guillain Barré Syndrome in humans is characterised by
ascending paralysis. It is often associated with preceding
infections two to four weeks prior to nadir and is fatal in five
percent of cases.
Antibodies specific to several nerve components
are frequently associated with clinical symptoms in GBS. These
antibodies were found to be specific to various gangliosides and
ganglioside complexes. It was also found that antibody reactivity
to gangliosides is affected by membrane components. The most
prevalent (20-30%) immunoglobulin in GBS is anti-GM1 (20-30%),
which also binds to the LPS of the PEN O:19 Campylobacter jejuni
serotype. This is the most common infectious agent associated with
GBS and emphasizes the importance of infection and anti-ganglioside
antibodies in disease development. Intravenous infusion of pooled
immunoglobulin from healthy donors, also called intravenous
immunoglobulin (IVIg), halves the severity of disease
manifestation. The action mechanism of IVIg in curing GBS is not
clear, but intravenous immunoglobulin was shown to neutralize
anti-ganglioside binding activity and its pathogenic effects. It
was further found that anti-idiotypic
antibodies in IVIg inhibit
anti-ganglioside antibody activity. Treatment with IVIg is not
equally effective in all GBS cases, which might be due to the
inability of IVIg to neutralize anti-ganglioside
antibodies in all
patients adequately. Therefore, the treatment of GBS with IVIg
needs to be better understood in order to improve its use as a cure
for GBS. This study confirmed previous findings that the
interaction of patient serum anti-GM1
antibodies and ganglioside
auto-antigens is greatly impaired by components in healthy serum.
Bound anti-GM1
antibodies could be displaced by (presumably)
anti-idiotypic
antibodies from healthy donor serum. This study
found that the displacement potential between donor sera differs.
Anti-GM1 antibody displacement was found to be dependent on the
character of both anti-GM1 and anti-idiotypic antibody. This
demonstrated the feasibility of improving the efficiency of
treatment by IVIg by sourcing it from only those sera that test
best for displacing auto-
antibodies from their ganglioside antigens
in ELISA. IVIg selection may therefore greatly benefit from the use
of recombinant phage display
antibodies to distinguish between the
various types of GBS for treatment. To develop a method to
characterize anti-ganglioside
antibodies sensitively, an evanescent
field biosensor was employed in which gangliosides were presented
in a liposome environment. This provided a more physiological way
of antibody antigen recognition. The optimized method determined
the ganglioside binding specificity of purified IgG from a GBS
patient, and mouse monoclonal anti-GM1 and anti-GD1a
antibodies
accurately. The results compared well with those from ELISA. The
results obtained with purified IgG were far better than that
obtained with whole serum analysis. This could be due to
non-specific binding or the presence of inhibiting anti-idiotypic
antibodies in…
Advisors/Committee Members: Prof J A Verschoor (advisor).
Subjects/Keywords: Antibody-antigen interactions;
Antibodies;
UCTD
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Van der Merwe, H. (2008). A resonant
mirror biosensor approach to understand antibody-antigen
interactions in Guillain Barré Syndrome. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/23995
Chicago Manual of Style (16th Edition):
Van der Merwe, Hermanus. “A resonant
mirror biosensor approach to understand antibody-antigen
interactions in Guillain Barré Syndrome.” 2008. Masters Thesis, University of Pretoria. Accessed March 02, 2021.
http://hdl.handle.net/2263/23995.
MLA Handbook (7th Edition):
Van der Merwe, Hermanus. “A resonant
mirror biosensor approach to understand antibody-antigen
interactions in Guillain Barré Syndrome.” 2008. Web. 02 Mar 2021.
Vancouver:
Van der Merwe H. A resonant
mirror biosensor approach to understand antibody-antigen
interactions in Guillain Barré Syndrome. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2263/23995.
Council of Science Editors:
Van der Merwe H. A resonant
mirror biosensor approach to understand antibody-antigen
interactions in Guillain Barré Syndrome. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/23995

University of Toronto
20.
Lau, Esther.
Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.
Degree: 2013, University of Toronto
URL: http://hdl.handle.net/1807/70019
► Receptor tyrosine kinases or RTKs are an important class of signaling proteins that are frequently deregulated in cancer and other diseases. Based on a series…
(more)
▼ Receptor tyrosine kinases or RTKs are an important class of signaling proteins that are frequently deregulated in cancer and other diseases. Based on a series of genetic screens to identify fitness genes across a compendium of cancer cell lines, the Moffat lab identified the receptor kinases HER3/ERBB3 and PTK7/CCK4 as important for the fitness of breast, pancreatic, ovarian, and colon cancer cell lines. Interestingly, both of the RTKs are predicted to have no kinase activity, suggesting that they are not likely amenable to direct enzymatic inhibition by small molecule chemical inhibitors. Using emergent synthetic antibody phage-display technology, I describe my efforts to generate and characterize synthetic antibodies targeting the extracellular regions of the receptor tyrosine kinases HER3 and PTK7.
MAST
Advisors/Committee Members: Moffat, Jason, Molecular and Medical Genetics.
Subjects/Keywords: HER3; PTK7; synthetic antibodies; cancer
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lau, E. (2013). Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70019
Chicago Manual of Style (16th Edition):
Lau, Esther. “Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.” 2013. Masters Thesis, University of Toronto. Accessed March 02, 2021.
http://hdl.handle.net/1807/70019.
MLA Handbook (7th Edition):
Lau, Esther. “Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7.” 2013. Web. 02 Mar 2021.
Vancouver:
Lau E. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/1807/70019.
Council of Science Editors:
Lau E. Generation of Human Synthetic Antibodies to Investigate the Biological Functions of the Receptor Tyrosine Kinases HER3 and PTK7. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/70019

University of Wisconsin – La Cross
21.
Jaedike, Alicia.
Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection.
Degree: 2019, University of Wisconsin – La Cross
URL: http://digital.library.wisc.edu/1793/80314
► Influenza is a highly contagious viral pathogen that causes respiratory illness in humans and animals. It is a major public health threat, resulting in three…
(more)
▼ Influenza is a highly contagious viral pathogen that causes respiratory illness in humans and animals. It is a major public health threat, resulting in three to five million yearly illnesses and up to 300,000 deaths worldwide. While the basic characteristics of this disease have been identified, there are still a variety of factors that remain unclear. The use of animal models to study influenza allows an accurate representation of influenza illness in humans. With animal models, there are limitations including size, maintenance costs, and clinical manifestations compared to a human infection. One of the best animal options is the ferret. Ferrets are small, have similar respiratory anatomy to humans, and can transmit influenza viruses between animals. The ability to use the ferret as a model to study influenza is hindered due to the limited availability of reagents. In order to study the immune response to influenza, reagents like monoclonal
antibodies are needed. Monoclonal
antibodies are useful for studying the immune response, including influenza pathogenesis, immune cell responders, and vaccine efficacy. The object of this study is to generate
antibodies specific for ferret immune cell proteins to study influenza and other diseases.
Advisors/Committee Members: Wilker, Peter.
Subjects/Keywords: Monoclonal antibodies; Influenza; Ferrets
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Jaedike, A. (2019). Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection. (Thesis). University of Wisconsin – La Cross. Retrieved from http://digital.library.wisc.edu/1793/80314
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jaedike, Alicia. “Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection.” 2019. Thesis, University of Wisconsin – La Cross. Accessed March 02, 2021.
http://digital.library.wisc.edu/1793/80314.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jaedike, Alicia. “Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection.” 2019. Web. 02 Mar 2021.
Vancouver:
Jaedike A. Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection. [Internet] [Thesis]. University of Wisconsin – La Cross; 2019. [cited 2021 Mar 02].
Available from: http://digital.library.wisc.edu/1793/80314.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jaedike A. Generation of monoclonal antibodies to ferret immune cell proteins in support of the ferret model to study human influenza infection. [Thesis]. University of Wisconsin – La Cross; 2019. Available from: http://digital.library.wisc.edu/1793/80314
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center
22.
Desmond, Angela.
New Approaches to the Development of Peptoid Vaccines.
Degree: 2015, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1573
► The ideal prophylactic vaccine against a toxin or pathogen should elicit the production of broadly protective antibodies against conserved epitopes. However, the epitopes that elicit…
(more)
▼ The ideal prophylactic vaccine against a toxin or pathogen should elicit the production of broadly protective
antibodies against conserved epitopes. However, the epitopes that elicit these
antibodies are often not immunodominant and even when they are, characterizing and synthesizing them can be difficult, particularly if they are conformational. The long-term goal of this work was to develop prophylactic vaccines that elicit such
antibodies without epitope characterization. To develop such a vaccine platform, it was hypothesized that screening large one-bead-one-compound libraries of synthetic compounds with monoclonal
antibodies that have already been shown to be broadly protective against a toxin or pathogen would allow the identification of mimetic B cell epitopes. For this platform, peptoids were chosen to construct one-bead-one-compound libraries. Peptoids are N-oligosubstituted glycines that resemble peptides but bear their side groups on backbone nitrogens instead of carbons. This renders them protease resistant and enormously diverse, since they are not restricted to the twenty standard amino acids. Furthermore, previous work had demonstrated that a monoclonal antibody could be used to screen libraries of peptoids. Moreover, while peptoids themselves were not immunogenic, the attachment of peptoids to carrier proteins using a linker elicited
antibodies against the peptoid/linker. Such T-cell dependent antigens elicited high-affinity, class-switched
antibodies. The goal of this dissertation research was to continue optimizing the magnetic and color-based assays by which peptoid vaccine candidates could be identified and to screen libraries with neutralizing monoclonal
antibodies against West Nile virus and murine norovirus type 1. In addition, the immunogenicity of peptoids was further examined by designing a peptoid-carrier, using it to immunize rabbits, and demonstrating that anti-peptoid
antibodies could be affinity-purified from the resulting antisera. This antibody was then used in further optimization of the magnetic screening assay to ensure that future screens will efficiently and specifically identify the best vaccine candidates.
Advisors/Committee Members: Pfeiffer, Julie K., Vitetta, Ellen S., Levine, Beth, Niederkorn, Jerry Y., Ward, E. Sally.
Subjects/Keywords: Antibodies, Monoclonal; Peptoids; Vaccines
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Desmond, A. (2015). New Approaches to the Development of Peptoid Vaccines. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1573
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Desmond, Angela. “New Approaches to the Development of Peptoid Vaccines.” 2015. Thesis, University of Texas Southwestern Medical Center. Accessed March 02, 2021.
http://hdl.handle.net/2152.5/1573.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Desmond, Angela. “New Approaches to the Development of Peptoid Vaccines.” 2015. Web. 02 Mar 2021.
Vancouver:
Desmond A. New Approaches to the Development of Peptoid Vaccines. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2015. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2152.5/1573.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Desmond A. New Approaches to the Development of Peptoid Vaccines. [Thesis]. University of Texas Southwestern Medical Center; 2015. Available from: http://hdl.handle.net/2152.5/1573
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center
23.
Velmurugan, Ramraj.
Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.
Degree: 2017, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/7195
► The file named "VELMURUGAN-DISSERTATION-2017.pdf" is the primary dissertation file. Eight (8) supplemental files are also available and may be viewed individually.
The use of monoclonal…
(more)
▼ The file named "VELMURUGAN-DISSERTATION-2017.pdf" is the primary dissertation file. Eight (8) supplemental files are also available and may be viewed individually.
The use of monoclonal antibodies represents a rapidly expanding area for cancer therapy. One of the main mechanisms of action of these antibodies is Fcγ receptor-mediated engagement of macrophages and other immune cells. When macrophages engage tumor cells opsonized with antibody molecules, they can perform trogocytosis, the process of internalizing fragments of the target cell, or phagocytosis, the internalization of entire cancer cells. This study first establishes whether the process of trogocytosis can lead to cancer cell death. A variety of microscopy and flow cytometric assays were used to quantify the levels of trogocytosis and cell death, in co-cultures of macrophages and cancer cells. Using HER2-overexpressing breast cancer cell lines and anti-HER2 antibodies, we show that persistent trogocytosis can lead to the killing of cancer cells. The mechanism of trogocytosis was also explored using multifocal plane microscopy (MUM). Imaging the process of trogocytosis using MUM revealed that it proceeds through the macrophage-mediated extrusion of tubular structures of the target cell membrane. This membrane-tubulation results in the preferential uptake of the membrane components from the target cell. The study also investigated the maturation pathway followed by phagosomes containing entire cancer cells. A vacuole-like structure associates with these phagosomes, which whilst also lysosomal in nature, displays characteristics distinct from the phagosome itself. The interface between the vacuole and the phagosome is impermeable to certain solutes as observed through microscopy. Further, the size of the phagosome-associated vacuole is affected by inhibition of the mTOR pathway. Use of advanced microscopy techniques such as MUM in these and other biological problems provides mechanistic insight at the spatiotemporal level. To further develop the algorithms involved in MUM data processing, I have therefore also explored various non-parametric methods of estimating the axial location of point sources from MUM data. A new non-parametric method is proposed, which uses multiple intensities calculated from each image of a point source in MUM data. The performance of this approach is compared with other non-parametric methods through simulations and Fisher information calculations. The effectiveness of this method on experimental data is also evaluated.
Advisors/Committee Members: Li, Wen-Hong, Ward, E. Sally, Ober, Raimund J., Alexandrakis, Georgios, Pasare, Chandrashekhar.
Subjects/Keywords: Antibodies; Breast Neoplasms; Macrophages; Phagocytosis
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Velmurugan, R. (2017). Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/7195
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Velmurugan, Ramraj. “Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.” 2017. Thesis, University of Texas Southwestern Medical Center. Accessed March 02, 2021.
http://hdl.handle.net/2152.5/7195.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Velmurugan, Ramraj. “Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies.” 2017. Web. 02 Mar 2021.
Vancouver:
Velmurugan R. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2017. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2152.5/7195.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Velmurugan R. Using Advanced Microscopy Techniques for the Study of Macrophage-Cancer Cell Interactions in the Presence of Therapeutic Antibodies. [Thesis]. University of Texas Southwestern Medical Center; 2017. Available from: http://hdl.handle.net/2152.5/7195
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center
24.
Sullivan, Laura Anne.
Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.
Degree: 2012, University of Texas Southwestern Medical Center
URL: http://hdl.handle.net/2152.5/1040
► Angiogenesis is the development of blood vessels from a pre-existing vascular network. This process is essential during growth, development and wound healing and plays a…
(more)
▼ Angiogenesis is the development of blood vessels from a pre-existing vascular network. This process is essential during growth, development and wound healing and plays a critical role in the growth and progression of cancer. Initial tumor size is restricted by the diffusion capacity of oxygen and nutrients from surrounding blood vessels. Therefore, to progress beyond a volume of several millimeters, a tumor must stimulate angiogenesis to generate a vascular network that will supply the tumor with the necessary blood, oxygen and nutrients that will allow for continued growth, invasion and metastasis.
Over forty years ago, Judah Folkman hypothesized that targeting tumor angiogenesis would be beneficial for cancer patients. One of the first targets for this new class of drugs was vascular endothelial growth factor (VEGF) a predominant mediator of physiological and pathological angiogenesis. Bevacizumab (Avastin®, Genentech/Roche), a humanized monoclonal antibody that recognizes human VEGF and blocks VEGF from binding to VEGF receptor (VEGFR) 1 and 2, was the first anti-angiogenic drug approved by the United States Food and Drug Administration for the treatment of cancer and remains the gold standard for this class of therapeutics. The Brekken laboratory, in collaborations with Peregrine Pharmaceuticals and Affitech A/S has generated a fully human monoclonal antibody, r84 that recognizes mouse and human VEGF and blocks VEGF binding only to VEGFR2. The data presented in the first half of this dissertation demonstrate the specificity of r84 for VEGF in vitro and in vivo, the efficacy of r84 to control tumor growth and the superior safety profile of r84 as compared to bevacizumab.
Although anti-angiogenic therapy was highly anticipated to have great success in patients, overall results have been somewhat disappointing with modest improvements in patient progression free survival and few improvements to overall survival. In addition, with the expanding use of anti-angiogenic drugs such as bevacizumab and a host of receptor tyrosine kinase inhibitors in the clinic, it is becoming increasingly apparent that not all tumors respond or maintain sensitivity to treatment. Therefore, it is increasingly important to identify mechanisms of resistance to anti-angiogenic therapy so that new drug targets can be identified and/or patients can be appropriately screened for markers that can predict for resistance or sensitivity to anti-angiogenic therapy de novo. Non-small cell lung cancer (NSCLC), the most common form of lung cancer, claims the most new diagnoses and cancer-related deaths than any other cancer worldwide and the therapeutic options currently available for this disease, including bevacizumab have done little to change this statistic. The latter half of this thesis focuses on the in vivo screening of human NSCLC cell lines to identify mechanisms of resistance to the anti-angiogenic monoclonal
antibodies bevacizumab and r84 in non-small cell lung cancer.
Advisors/Committee Members: Brekken, Rolf A..
Subjects/Keywords: Antineoplastic Agents; Neoplasms; Antibodies, Monoclonal
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sullivan, L. A. (2012). Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1040
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sullivan, Laura Anne. “Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.” 2012. Thesis, University of Texas Southwestern Medical Center. Accessed March 02, 2021.
http://hdl.handle.net/2152.5/1040.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sullivan, Laura Anne. “Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance.” 2012. Web. 02 Mar 2021.
Vancouver:
Sullivan LA. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2012. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2152.5/1040.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sullivan LA. Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance. [Thesis]. University of Texas Southwestern Medical Center; 2012. Available from: http://hdl.handle.net/2152.5/1040
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University
25.
Marcus, Claire E.
The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility.
Degree: MS, Pathology, 2020, Boston University
URL: http://hdl.handle.net/2144/41291
► Antisperm antibodies (ASA) are thought to be a predominate cause of immune infertility by interfering with various aspects of sperm function in both the male…
(more)
▼ Antisperm
antibodies (ASA) are thought to be a predominate cause of immune infertility by interfering with various aspects of sperm function in both the male and the female reproductive tracts. The precise mechanism by which these
antibodies contribute to infertility, as well as their etiology, remains to be established. ASA are present in a variety of biological substrates, such as genital tract secretions, and the blood sera of both males and females. Although not all ASA underly infertility, a substantial body of research suggests that certain ASA, referred to as sperm immobilizing
antibodies (SI-Abs) and sperm agglutinating
antibodies, significantly impair sperm transportation in the female reproductive tract. High titers of sperm agglutinating or sperm immobilizing
antibodies have been associated with reproductive failure. CD52g is a GPI anchored glycoprotein found on mature sperm and in seminal plasma (SP).
Antibodies against a male reproductive tract-specific epitope of CD52g are known to readily agglutinate sperm. The current study sought to develop an ELISA to quantify the prevalence of CD52g
antibodies in the sera of male and female patients with infertility, and to determine if there was a correlation between the prevalence of CD52g
antibodies and the prevalence of sperm agglutinating
antibodies in the sera of these patients. Ultimately, CD52g
antibodies were only detected in the sera of patients (21%) with sperm agglutinating
antibodies. While detecting CD52g
antibodies in sera via an ELISA proved challenging, the results of this study corroborate research demonstrating that CD52g
antibodies have a remarkable capacity to agglutinate sperm. Elucidation of the mechanisms underlying this immune response would advance our understanding of immune modulation in human reproductive tracts, further the diagnosis of immune infertility, and are currently providing the basis for the development of a potent dual purpose immunocontraceptive, that both prevents unintended pregnancy, and prevents the transmission of sexually transmitted infections (STIs).
Advisors/Committee Members: Anderson, Deborah (advisor), Duffy, Elizabeth (advisor).
Subjects/Keywords: Immunology; Antisperm antibodies; Infertility
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Marcus, C. E. (2020). The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/41291
Chicago Manual of Style (16th Edition):
Marcus, Claire E. “The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility.” 2020. Masters Thesis, Boston University. Accessed March 02, 2021.
http://hdl.handle.net/2144/41291.
MLA Handbook (7th Edition):
Marcus, Claire E. “The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility.” 2020. Web. 02 Mar 2021.
Vancouver:
Marcus CE. The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility. [Internet] [Masters thesis]. Boston University; 2020. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2144/41291.
Council of Science Editors:
Marcus CE. The development of an ELISA for the quantification of antibodies against CD52G, a sperm coating glycoprotein, in the sera of patients with infertility. [Masters Thesis]. Boston University; 2020. Available from: http://hdl.handle.net/2144/41291

University of California – Santa Cruz
26.
Fedechkin, Stanislav.
Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.
Degree: Chemistry, 2018, University of California – Santa Cruz
URL: http://www.escholarship.org/uc/item/21c5j93j
► Respiratory syncytial virus (RSV) is a top cause of severe lower respiratory tract disease and mortality in young children and the elderly. The viral envelope…
(more)
▼ Respiratory syncytial virus (RSV) is a top cause of severe lower respiratory tract disease and mortality in young children and the elderly. The viral envelope G glycoprotein contributes to pathogenesis through its roles in host cell attachment and modulation of host immunity. Although the G glycoprotein is a target of protective RSV-neutralizing antibodies, its development as a vaccine antigen has been hindered by its heterogeneous glycosylation and sequence variability outside a conserved central domain (CCD). We describe the cocrystal structures of three high-affinity broadly neutralizing human monoclonal antibodies bound to the RSV G CCD. All three antibodies bind to conformational epitopes that span a highly conserved surface, illuminating an important region of vulnerability. We further show that isolated RSV G CCD activates the chemokine receptor CX3CR1 and that antibodies block this activity. These studies provide a template for rational vaccine design targeting this key contributor to RSV disease.
Subjects/Keywords: Biochemistry; Antibodies; Attachment; G; RSV
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fedechkin, S. (2018). Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. (Thesis). University of California – Santa Cruz. Retrieved from http://www.escholarship.org/uc/item/21c5j93j
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Fedechkin, Stanislav. “Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.” 2018. Thesis, University of California – Santa Cruz. Accessed March 02, 2021.
http://www.escholarship.org/uc/item/21c5j93j.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Fedechkin, Stanislav. “Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design.” 2018. Web. 02 Mar 2021.
Vancouver:
Fedechkin S. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. [Internet] [Thesis]. University of California – Santa Cruz; 2018. [cited 2021 Mar 02].
Available from: http://www.escholarship.org/uc/item/21c5j93j.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Fedechkin S. Structures Of Respiratory Syncytial Virus G Antigen Bound To Broadly Neutralizing Antibodies For Vaccine And Therapeutic Design. [Thesis]. University of California – Santa Cruz; 2018. Available from: http://www.escholarship.org/uc/item/21c5j93j
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University
27.
Bhanot, Anisha.
Maternal antibodies in autism: what is known and future directions.
Degree: MS, Medical Sciences, 2018, Boston University
URL: http://hdl.handle.net/2144/30896
► Autism spectrum disorder (ASD) refers to a highly prevalent neuropsychiatric disorder, currently affecting one in every 68 children. ASD is understood as a heterogeneous disorder,…
(more)
▼ Autism spectrum disorder (ASD) refers to a highly prevalent neuropsychiatric disorder, currently affecting one in every 68 children. ASD is understood as a heterogeneous disorder, and individuals with this condition vary considerably in terms of symptom presentation. This heterogeneity contributes to the difficulty faced by researchers and clinicians in trying to determine the precise underlying mechanisms and treatment for this condition. Furthermore, it remains unknown whether the variations in symptom manifestation are attributed to differences in underlying etiologies of the disorder or other factors as yet to be identified.
Currently, it is believed that ASD is likely due to the interaction between different genetic and environmental factors. The maternal immune system is one example of where the environment may act upon genetic predispositions and lead to altered fetal brain development. Considering the importance of the immune environment during fetal development, maternal
antibodies (Abs) directed against fetal proteins have been considered as potentially playing a critical function in the pathology of ASD.
This thesis examines the literature focused on the role of maternal Abs in fetal development and their impact on the neuropathology of ASD. Studies have collected samples from mothers of children diagnosed with ASD and examined the reactivity patterns of the maternal Abs against fetal proteins. Through review and inspection of methodologies and results, this thesis highlights the important insights obtained as well as proposes possible reasons for the disparity in findings. Lastly, this thesis proposes future directions and therapeutic implications of identifying the maternal Abs that could be involved in at least a subset of ASD cases.
Advisors/Committee Members: Bauman, Margaret (advisor), McDougle, Christopher (advisor).
Subjects/Keywords: Neurosciences; Antibodies; Autism; Immunology; Neurodevelopment
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bhanot, A. (2018). Maternal antibodies in autism: what is known and future directions. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/30896
Chicago Manual of Style (16th Edition):
Bhanot, Anisha. “Maternal antibodies in autism: what is known and future directions.” 2018. Masters Thesis, Boston University. Accessed March 02, 2021.
http://hdl.handle.net/2144/30896.
MLA Handbook (7th Edition):
Bhanot, Anisha. “Maternal antibodies in autism: what is known and future directions.” 2018. Web. 02 Mar 2021.
Vancouver:
Bhanot A. Maternal antibodies in autism: what is known and future directions. [Internet] [Masters thesis]. Boston University; 2018. [cited 2021 Mar 02].
Available from: http://hdl.handle.net/2144/30896.
Council of Science Editors:
Bhanot A. Maternal antibodies in autism: what is known and future directions. [Masters Thesis]. Boston University; 2018. Available from: http://hdl.handle.net/2144/30896

University of Dundee
28.
Wong, Julin.
Targeting c-Met for therapy.
Degree: PhD, 2011, University of Dundee
URL: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820
► c-Met is a tyrosine receptor kinase which is activated by its only ligand, the hepatocyte growth factor (HGF). Activation of c-Met leads to a wide…
(more)
▼ c-Met is a tyrosine receptor kinase which is activated by its only ligand, the hepatocyte growth factor (HGF). Activation of c-Met leads to a wide spectrum of biological activities such as motility, angiogenesis, morphogenesis, cell survival and cell regeneration. c-Met and HGF knock-out mice are embryonic lethal. During embryogenesis, c-Met is required for liver, kidney and skeletal muscles development. In adult tissues, c-Met is involved in wound healing and hepatocyte regeneration. c-Met is abnormally activated in many tumours types. Aberrant c-Met activation was found to induce tumour development, tumour cell migration and invasion, and the worst and final step in cancer progression, metastasis. In addition, c-Met activation in cells was also shown to confer resistance to apoptosis induced by UV damage or chemotherapeutic drugs. c-Met is thus an attractive target for drug development. This study describes the development of monoclonal antibodies against c-Met as therapeutic molecules in cancer treatment/diagnostics. Antibodies were raised against the a-chain of c-Met. 21 hybridoma clones were single-cell cloned and subjected to preliminary monoclonal antibody characterisation. 11 monoclonal antibodies were finally selected for ascites production and antibody purification. These purified antibodies were characterised by Western blotting, immunofluorescence staining, functional assays (ERK phosphorylation and cell scatter) and for their ability to recognise native c-Met by flow cytometry. Some of the anti-a-chain c-Met antibodies perform better in Western blotting and immunofluorescence staining than the presently-available commercial antibodies. The Mab 2.1 and 13.1 bind strongly to native c-Met in flow cytometry and may be potential candidates for antibody therapy and cancer diagnostics.
Subjects/Keywords: 616.994; c-Met; Antibodies; HGFR
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wong, J. (2011). Targeting c-Met for therapy. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820
Chicago Manual of Style (16th Edition):
Wong, Julin. “Targeting c-Met for therapy.” 2011. Doctoral Dissertation, University of Dundee. Accessed March 02, 2021.
https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820.
MLA Handbook (7th Edition):
Wong, Julin. “Targeting c-Met for therapy.” 2011. Web. 02 Mar 2021.
Vancouver:
Wong J. Targeting c-Met for therapy. [Internet] [Doctoral dissertation]. University of Dundee; 2011. [cited 2021 Mar 02].
Available from: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820.
Council of Science Editors:
Wong J. Targeting c-Met for therapy. [Doctoral Dissertation]. University of Dundee; 2011. Available from: https://discovery.dundee.ac.uk/en/studentTheses/a670d71d-758a-48b2-aa5b-2255b2373d7e ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578820
29.
YAP PENG KANG.
STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.
Degree: 1996, National University of Singapore
URL: https://scholarbank.nus.edu.sg/handle/10635/153065
Subjects/Keywords: Monoclonal antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
KANG, Y. P. (1996). STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/153065
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
KANG, YAP PENG. “STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.” 1996. Thesis, National University of Singapore. Accessed March 02, 2021.
https://scholarbank.nus.edu.sg/handle/10635/153065.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
KANG, YAP PENG. “STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION.” 1996. Web. 02 Mar 2021.
Vancouver:
KANG YP. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. [Internet] [Thesis]. National University of Singapore; 1996. [cited 2021 Mar 02].
Available from: https://scholarbank.nus.edu.sg/handle/10635/153065.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
KANG YP. STRATEGIES FOR IMPROVEMENT IN MONOCLONAL ANTIBODY PRODUCTION. [Thesis]. National University of Singapore; 1996. Available from: https://scholarbank.nus.edu.sg/handle/10635/153065
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University
30.
Setyabudi, Mariane.
Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies.
Degree: MS, Clinical Laboratory Sciences, 2008, Michigan State University
URL: http://etd.lib.msu.edu/islandora/object/etd:16644
Subjects/Keywords: Receptor antibodies
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Setyabudi, M. (2008). Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:16644
Chicago Manual of Style (16th Edition):
Setyabudi, Mariane. “Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies.” 2008. Masters Thesis, Michigan State University. Accessed March 02, 2021.
http://etd.lib.msu.edu/islandora/object/etd:16644.
MLA Handbook (7th Edition):
Setyabudi, Mariane. “Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies.” 2008. Web. 02 Mar 2021.
Vancouver:
Setyabudi M. Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies. [Internet] [Masters thesis]. Michigan State University; 2008. [cited 2021 Mar 02].
Available from: http://etd.lib.msu.edu/islandora/object/etd:16644.
Council of Science Editors:
Setyabudi M. Particle-based flow cytometry assay to detect anti-angiotensin II type I and type II receptor antibodies. [Masters Thesis]. Michigan State University; 2008. Available from: http://etd.lib.msu.edu/islandora/object/etd:16644
◁ [1] [2] [3] [4] [5] … [64] ▶
.